LOCUS       DBERMR010000001       115268 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.001, whole genome shotgun sequence.
ACCESSION   DBERMR010000001 DBERMR010000000
VERSION     DBERMR010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 115268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 115268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..115268
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.001"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            280..486
                     /locus_tag="ACL8A1_000001"
     CDS             280..486
                     /locus_tag="ACL8A1_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1200865.1"
                     /translation="MGEEEMRNKMIIAVSLVVAGVMTYLMFSGLDEDFYHFPWKVFAG
                     FGIMSWLVREGLKLVRDVKKEFEE"
     gene            483..677
                     /locus_tag="ACL8A1_000002"
     CDS             483..677
                     /locus_tag="ACL8A1_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1200866.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTDWFDQALLRQFIRGALGFGFM
                     IFVVFLMGMEWLKGESHDGG"
     gene            664..1548
                     /locus_tag="ACL8A1_000003"
     CDS             664..1548
                     /locus_tag="ACL8A1_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1200867.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRAYLPQENVIVQKLKVKELIAFFQRIY
                     PNPLSNQEIDQLLQFVKQQKEQLAEKLSGGQKRLFSFVLTLIGRPKIVFLDEPTASMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEQSNLVENWTLKQDSLQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            1550..2287
                     /locus_tag="ACL8A1_000004"
     CDS             1550..2287
                     /locus_tag="ACL8A1_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1200868.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GELANFMRDYLISMTAFSMMSTTILSFPAVLHTDKMNNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFTVGHFVRGVDMPLVKWVGAAILLIVGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAVGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            2287..3384
                     /locus_tag="ACL8A1_000005"
     CDS             2287..3384
                     /locus_tag="ACL8A1_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGS1200869.1"
                     /translation="MLERLKSIHYMFWASLIFMLFPILPVVIGELPAWHLLVDILFVV
                     TYLGVLITKNQRLSWLFWGLMLVYVAGNTAFVAGNYIWFFFFLSNLLIYHFGVRSLKS
                     LHVWTFLLAQVLVVGRLLIFQRIEVEFLVYMLVILTFVDLMTLGSVRIRLVEDLKEAQ
                     VKQNTQINLLLAENERNRIGQDLHDSLGHTFAMLSVKIDLALQLFQMQAYPQVEKELR
                     EIQQISKESMREVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     ESMASMILLELVTNIIKHAKASKAYLKLERTEKELILTVSDDGCGFAFLKGDELHTIR
                     DRVFPFSGEVSVISQKHPTEVQVRLPYKERN"
     gene            3386..3985
                     /locus_tag="ACL8A1_000006"
     CDS             3386..3985
                     /locus_tag="ACL8A1_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGS1200870.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSERLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSVADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            4410..5420
                     /gene="ccpA"
                     /locus_tag="ACL8A1_000007"
     CDS             4410..5420
                     /gene="ccpA"
                     /locus_tag="ACL8A1_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HGS1200871.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGIAYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            5484..5687
                     /locus_tag="ACL8A1_000008"
     CDS             5484..5687
                     /locus_tag="ACL8A1_000008"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1200872.1"
                     /translation="MNTIERTRRLVKGCATHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     SKHVFEVVDRTDEVSNHTYGKAT"
     gene            complement(5716..6678)
                     /locus_tag="ACL8A1_000009"
     CDS             complement(5716..6678)
                     /locus_tag="ACL8A1_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HGS1200873.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVNTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            6744..8132
                     /locus_tag="ACL8A1_000010"
     CDS             6744..8132
                     /locus_tag="ACL8A1_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGS1200874.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAADKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            complement(8167..8619)
                     /locus_tag="ACL8A1_000011"
     CDS             complement(8167..8619)
                     /locus_tag="ACL8A1_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HGS1200875.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            8781..9074
                     /locus_tag="ACL8A1_000012"
     CDS             8781..9074
                     /locus_tag="ACL8A1_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1200876.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVEVIMICQLESLVPLLYPSFIG
                     FLVFHVLYHLILFLVAKRSGRWDYLMIWGLFLMFNLLYDSFLGLLFLGLSFGM"
     gene            9230..10444
                     /locus_tag="ACL8A1_000013"
     CDS             9230..10444
                     /locus_tag="ACL8A1_000013"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HGS1200877.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            10589..10723
                     /gene="rpmH"
                     /locus_tag="ACL8A1_000014"
     CDS             10589..10723
                     /gene="rpmH"
                     /locus_tag="ACL8A1_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HGS1200878.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            10899..11651
                     /locus_tag="ACL8A1_000015"
     CDS             10899..11651
                     /locus_tag="ACL8A1_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HGS1200879.1"
                     /translation="MCPVIILFYYKVEVAGDGTATITFPDGSVVTILGKDTVQQSAKG
                     ESVTQEATPEYKLENTPGGDKGGNTGSSDANANEGGGSQAGGSAHTGSQNSAQSQASK
                     QLATEKESAKNAIEKAAKNKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTM
                     EDVKEAETIGVQAIAMVTVPKRPVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPN
                     TGSASSAALASLGLVVATSGFALLGRKTRRRK"
     gene            11957..12730
                     /locus_tag="ACL8A1_000016"
     CDS             11957..12730
                     /locus_tag="ACL8A1_000016"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HGS1200880.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIE
                     HALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHSKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            12730..13290
                     /gene="rnmV"
                     /locus_tag="ACL8A1_000017"
     CDS             12730..13290
                     /gene="rnmV"
                     /locus_tag="ACL8A1_000017"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HGS1200881.1"
                     /translation="MKERISQVIVVEGRDDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            13362..14261
                     /locus_tag="ACL8A1_000018"
     CDS             13362..14261
                     /locus_tag="ACL8A1_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS1200882.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLAYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMPQIIEKEAYSINDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTQIENYFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALRVERDFSLAESFYQSSKTFAK
                     LIGDGFLVKKLTEEWQEDVKKYL"
     gene            14581..14877
                     /locus_tag="ACL8A1_000019"
     CDS             14581..14877
                     /locus_tag="ACL8A1_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HGS1200883.1"
                     /translation="MKQTVKKLALVASIAATLGGSVAVASAAVQYPEGGVWTYGSGNG
                     GAYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     gene            14954..16984
                     /locus_tag="ACL8A1_000020"
     CDS             14954..16984
                     /locus_tag="ACL8A1_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HGS1200884.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETRFTYATYGEGKLPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSVYYQGLFTDYL
                     QNFSSESVEVTSQKHYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GARPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRDLMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            16986..17627
                     /locus_tag="ACL8A1_000021"
     CDS             16986..17627
                     /locus_tag="ACL8A1_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1200885.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            17620..17928
                     /locus_tag="ACL8A1_000022"
     CDS             17620..17928
                     /locus_tag="ACL8A1_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1200886.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFERMFRENC
                     "
     gene            18059..18931
                     /gene="rsmA"
                     /locus_tag="ACL8A1_000023"
     CDS             18059..18931
                     /gene="rsmA"
                     /locus_tag="ACL8A1_000023"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HGS1200887.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            18933..19811
                     /gene="rsgA"
                     /locus_tag="ACL8A1_000024"
     CDS             18933..19811
                     /gene="rsgA"
                     /locus_tag="ACL8A1_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HGS1200888.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            19822..20478
                     /gene="rpe"
                     /locus_tag="ACL8A1_000025"
     CDS             19822..20478
                     /gene="rpe"
                     /locus_tag="ACL8A1_000025"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HGS1200889.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTPVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGEVNERVQTLRKQLD
                     "
     gene            20441..21103
                     /locus_tag="ACL8A1_000026"
     CDS             20441..21103
                     /locus_tag="ACL8A1_000026"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HGS1200890.1"
                     /translation="MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLE
                     EDLPLALAVGDFDSVTEEERQVIQKRAQYFVQARPEKDDTDLELALLTIFEQNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMRQIEIEDGQNLIAYCSEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            21105..22361
                     /gene="rmuC"
                     /locus_tag="ACL8A1_000027"
     CDS             21105..22361
                     /gene="rmuC"
                     /locus_tag="ACL8A1_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HGS1200891.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVAVSDRLQEVRLELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYEVGDKDEIERCRKSLLASVKRFARDIRNKYI
                     APPRTTNFGILFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            22351..23292
                     /locus_tag="ACL8A1_000028"
     CDS             22351..23292
                     /locus_tag="ACL8A1_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HGS1200892.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMSNKIFAMDNRSFYKPDLD"
     gene            23559..24386
                     /gene="purR"
                     /locus_tag="ACL8A1_000029"
     CDS             23559..24386
                     /gene="purR"
                     /locus_tag="ACL8A1_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HGS1200893.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            24453..25703
                     /locus_tag="ACL8A1_000030"
     CDS             24453..25703
                     /locus_tag="ACL8A1_000030"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HGS1200894.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     ISLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            25829..26623
                     /gene="pflA"
                     /locus_tag="ACL8A1_000031"
     CDS             25829..26623
                     /gene="pflA"
                     /locus_tag="ACL8A1_000031"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HGS1200895.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(26698..27624)
                     /gene="yidC"
                     /locus_tag="ACL8A1_000032"
     CDS             complement(26698..27624)
                     /gene="yidC"
                     /locus_tag="ACL8A1_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HGS1200896.1"
                     /translation="MKSIKRFAFSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(27702..27980)
                     /locus_tag="ACL8A1_000033"
     CDS             complement(27702..27980)
                     /locus_tag="ACL8A1_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HGS1200897.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            28014..28754
                     /locus_tag="ACL8A1_000034"
     CDS             28014..28754
                     /locus_tag="ACL8A1_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HGS1200898.1"
                     /translation="MTIITSKANSVVKNAKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRNQLAAFPQTVWISEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVATGILIFHLS"
     gene            28810..29493
                     /locus_tag="ACL8A1_000035"
     CDS             28810..29493
                     /locus_tag="ACL8A1_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HGS1200899.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYLLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            29604..30596
                     /gene="asnA"
                     /locus_tag="ACL8A1_000036"
     CDS             29604..30596
                     /gene="asnA"
                     /locus_tag="ACL8A1_000036"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HGS1200900.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            30661..31200
                     /gene="rsmD"
                     /locus_tag="ACL8A1_000037"
     CDS             30661..31200
                     /gene="rsmD"
                     /locus_tag="ACL8A1_000037"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HGS1200901.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAKRELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            31190..31678
                     /gene="coaD"
                     /locus_tag="ACL8A1_000038"
     CDS             31190..31678
                     /gene="coaD"
                     /locus_tag="ACL8A1_000038"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HGS1200902.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATFLVRGLRNAADLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACHVPESILEEIRNEK
                     KD"
     gene            31662..32699
                     /locus_tag="ACL8A1_000039"
     CDS             31662..32699
                     /locus_tag="ACL8A1_000039"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HGS1200903.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKEILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            32757..32987
                     /locus_tag="ACL8A1_000040"
     CDS             32757..32987
                     /locus_tag="ACL8A1_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HGS1200904.1"
                     /translation="MVQLLFTLSSHMFFIYVSFYLLKDLVRWEKVLKVTDDNTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            33011..34294
                     /gene="murA"
                     /locus_tag="ACL8A1_000041"
     CDS             33011..34294
                     /gene="murA"
                     /locus_tag="ACL8A1_000041"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HGS1200905.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            34287..34475
                     /locus_tag="ACL8A1_000042"
     CDS             34287..34475
                     /locus_tag="ACL8A1_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HGS1200906.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            34514..35338
                     /gene="endA"
                     /locus_tag="ACL8A1_000043"
     CDS             34514..35338
                     /gene="endA"
                     /locus_tag="ACL8A1_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HGS1200907.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMPSAPNSPKTNLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            35567..36898
                     /locus_tag="ACL8A1_000044"
     CDS             35567..36898
                     /locus_tag="ACL8A1_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HGS1200908.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTNTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(37096..37166)
                     /locus_tag="ACL8A1_000045"
     tRNA            complement(37096..37166)
                     /locus_tag="ACL8A1_000045"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37132..37134),aa:Cys,seq:gca)
     gene            37582..41193
                     /gene="rpoB"
                     /locus_tag="ACL8A1_000046"
     CDS             37582..41193
                     /gene="rpoB"
                     /locus_tag="ACL8A1_000046"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HGS1200909.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TSEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVIAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            41226..44903
                     /gene="rpoC"
                     /locus_tag="ACL8A1_000047"
     CDS             41226..44903
                     /gene="rpoC"
                     /locus_tag="ACL8A1_000047"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HGS1200910.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKTEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            45014..45427
                     /gene="ndk"
                     /locus_tag="ACL8A1_000048"
     CDS             45014..45427
                     /gene="ndk"
                     /locus_tag="ACL8A1_000048"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HGS1200911.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYLPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEVIQNVVHGSDSEESAKREIALWF"
     gene            complement(45936..46214)
                     /locus_tag="ACL8A1_000049"
     CDS             complement(45936..46214)
                     /locus_tag="ACL8A1_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComA"
                     /protein_id="HGS1200912.1"
                     /translation="MKKFDNYIIEKPCDSNSDKLQKILIIENLVDDILQFPLRINNSV
                     GEIFLLQPFQKKTIFIPCYFEEDIVKVKDDDKVEWNLLEFQKFRAFLA"
     gene            complement(46355..46987)
                     /locus_tag="ACL8A1_000050"
     CDS             complement(46355..46987)
                     /locus_tag="ACL8A1_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1200913.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLESYDGMIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLAYLDLDKRIFELSGGESQRVALAKVILKNPPLILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            complement(46989..47756)
                     /locus_tag="ACL8A1_000051"
     CDS             complement(46989..47756)
                     /locus_tag="ACL8A1_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HGS1200914.1"
                     /translation="MSQKLNHLDVGEFVLLLPEHLRSEEEHYKSVFEDDLTSRMSSQD
                     ERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQLTGPQSILFWIDAVQ
                     NYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVMLAGAVLGIAT
                     SILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLGFVASVFLQVE
                     ILVAFLVSLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
     gene            complement(47807..49546)
                     /locus_tag="ACL8A1_000052"
     CDS             complement(47807..49546)
                     /locus_tag="ACL8A1_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HGS1200915.1"
                     /translation="MKIMKKKYWTLAILFFCLFNNSVTAQEIPKNLDGNITHTQTSES
                     FSESDEKQVDYSNKNQEEVDQNKFRIQIDKTELFVTTDKHLEKNCCKLELEPQINNDI
                     VNSESNNLLGEDNLDNKIKENVSHLDNRGGNIEHDKDNLESSIVRKYEWDIDKVTGGG
                     ESYKLYSKSNSKVSIAILDSGVDLQNTGLLKNLSNHSKNYVPNKGYLGKEEGEEGIIS
                     DIQDRLGHGTAVVAQIVGDDNINGVNPHVNINVYRIFGKSSASPDWIVKAIFDAVDDG
                     NDIINLSTGQYLMIDGEYEDGTNDFETFLKYKKAIDYANQKGVIIVAALGNDSLNVSN
                     QSDLLKLISSRKKVRKPGLVVDVPSYFSSTISVGGIDRLGNLSDFSNKGDSDAIYAPA
                     GSTLSLSELGLNNFINAEKYKEDWIFSATLGGYTYLYGNSFAAPKVSGAIAMIIDKYK
                     LKDQPYNYMFVKKILEETLPVKNGIKVLNIPNVLRYDLNMLQLEYKNEQSWDSFIDNV
                     NLIELEERIQTTIGIKQINTHNIITIAREGYSQNYLPNTSENTYNSLQVSLVGVLLLF
                     ISMVNILWAKKSK"
     gene            complement(49570..51678)
                     /locus_tag="ACL8A1_000053"
     CDS             complement(49570..51678)
                     /locus_tag="ACL8A1_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HGS1200916.1"
                     /translation="MKIPMIYQMENSECGLACCAMILNYFKYEISLNELREIYPSSRS
                     GYSLLSISKVLGDFNISSHAFKASVRDLKPLSFPLICFWESSHFIILEKISKNKFYIL
                     DPAKGRQRMSISEFERHYSNIILTFKKLDSFMSRKDNKKSPVLKYFFKYRNKLGILFF
                     VTALLYVIQSLVPIANRYIIDTNFKDDSYSSRMLFTILFIFTVSFSLMYLLRQIYVAS
                     LKYIMDKEISYDFMKHLIYLPYSFYEKRTLGDILFRANSIVYIREILSNNFIAAILDL
                     LMIVVYAVVLFSFSKYMVIFLISLSLALSIVMYPIIKISKNLIDKNIKEKVNVQNITS
                     EVISKNSDIKLTGEEEFWINKWDNFNTKQLIIGRKLDIHLSIVSSITNVLQIILPVLT
                     LIVGVNIKTFEQLTLGQIVAISTVSPYFISPIISLSDNYIQLMLLKGYFLRIEDVFNT
                     KSELIPERVSQDIKFDKKIELKDIWYKYGLFDDYVLKGINVTIKKGETVAIVGESGSG
                     KSTLAKILLGLLEPNIGSIEVDGVEKEEIGQTLYRKIFGAVLQNSTLSYGTLRENLTF
                     GHFVSDEELMTNLNSIGLSNVVKSLPLGLETIIAEEGNNFSGGQQQMILLARCLLSKP
                     SVVVLDEATSSLDNLSQQITTSYLSEIGTTKILIAHRLDTIKSADKILVMHNGEIVEI
                     GTHRELLELGGIYKQLYSNN"
     gene            complement(51675..52604)
                     /locus_tag="ACL8A1_000054"
     CDS             complement(51675..52604)
                     /locus_tag="ACL8A1_000054"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HGS1200917.1"
                     /translation="MLKILNNSVIYLCLIPLLFLLLIIFPNDSLIYYFRLILISLISI
                     TLHELGHFLVGRCLSYKLEMLATPFFFYFRKKIYFKFPVLLAFGYCQMSNRNITNEKN
                     SDRNLVFYFFGGGGANLIVAILALLGFVPYASEFFILNIILFLVTVCLPIDGTDGNAI
                     REIVLYSKDSKTYQRFFANSLYNNPYITIDDFAKLTSKEKSFFSKFEKCLINLYFEVK
                     GEGSAFTIANHEVFDSNIQENIIHEYYKLLHKSSDWIIPQNLSLEEVVFTLSNYIYSK
                     NNKYLEKIKYLKKLVDFRQEEIIDFILNKEEVL"
     gene            complement(52585..53289)
                     /locus_tag="ACL8A1_000055"
     CDS             complement(52585..53289)
                     /locus_tag="ACL8A1_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HGS1200918.1"
                     /translation="MNKIFIYAGVRNHNSKTLEYTKRLSSIISSRNNVDISFRTPFNS
                     ELEISNSDSEELFKKGIDRQSNADDGGVIKKELLESDIIIISSPVYLQNVSVDTKNFI
                     ERIGGWSHLFRLAGKFVVTLDVAESNGSDNVSEYLRDIFSYMGGQILHQVSITNSLKD
                     IAEAQLMEATYKIEDVLEGKIKYKTTDYQERAYQTLKLILENYDSEHFEKMYWEKKRL
                     FEANSLEEWYYVENIK"
     gene            complement(53639..56629)
                     /gene="lanM"
                     /locus_tag="ACL8A1_000056"
     CDS             complement(53639..56629)
                     /gene="lanM"
                     /locus_tag="ACL8A1_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 lanthipeptide synthetase LanM"
                     /protein_id="HGS1200919.1"
                     /translation="MKYKIKNVHKTLFIKERYQILSERNDNSLNNFSLNNWRSTMSII
                     SEKTFEEMLKLNNYNKKIFSNTVNYDYTEEQIRLYSEYAKNSEWYKFAEGSFEIFRKH
                     PQRINFKKDFSRILEPFIINLLKLINNYLENKEYEWIDKNGNIFSSLVFYLEDLIYPW
                     IVKPLVLEINSLREKGLLEGESEQQRYKYFITLFDKEENILNFYNKYPVLLRQISESC
                     LRFYTYFIEILSNLENDFSVLEEELGLRGKLNDIKFGKGDTHSQGKTVLILFFDDAKI
                     VYKPKNLIINNSLNTIAEYIRKVDEKIRIRIPRTIAYSDHSYEEFIDYLPLEQKKKLP
                     EYYYNFGVLLAFIYLFNGSDIHFENLISYGDMPVIIDFETMLQQPLFDDKTGQSLLDT
                     LFHRVTRTLLLPTEGVKREDGLDVEMSALTGNFKKDAFNGQVLINLNTDKVKFDIGKI
                     DFEGGKNLPVRDGDIEFDKYIKDFKKGFRDFYLIFEELNKTEEFKMLLKANLYGLKTR
                     VLFRDTNSYASVLSFLYHPDFYEEMLDREKALENLWSNKFSNQGIVASECEQMRLLDI
                     PIFYTDTNINEIYDDFGNHFISLHQFSGYDYFMKYLPNNNLENLEKQLDIIEIYFPSF
                     DQRKKGYKLNLSNRKKLPNESLVTILHNEVENIADSLVINNIYDEYPIWSIPVNFKGS
                     KWCLEQTGNDMYRGRAGILLFMYYVKLLKDKSSYTQLYNKMLSAIPPSVSLSKTTFGL
                     TGDIGYFIVLSIIEDYDTNTLACLNYIKSVLTELEKTDFASVSNKSDYINGLLPLINT
                     LVRYYRRGIEKERVLRLVLSLGDTLYNDVSERDNSNFGYSFGHGLSGVIFTLKNIYKV
                     TRLKKYKELILQLVPREKIKIHSLKWCSGEFGTLVNHPQSVPEGIVASLDNDTFCHGS
                     MAIINFYIDYMKEMDTSMELESLLCSVVMNKSKEGEYRIVQTSNFPDFSLYTGVTGIA
                     YTFLRYIALNQNDKKIVIPSLLEI"
     gene            complement(56746..56934)
                     /gene="pneA2"
                     /locus_tag="ACL8A1_000057"
     CDS             complement(56746..56934)
                     /gene="pneA2"
                     /locus_tag="ACL8A1_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA2"
                     /protein_id="HGS1200920.1"
                     /translation="MKNDFVIGKSLKELSLEEMQLVYGGTDGADPRSTIICSATLSFI
                     ASYLGSAQTRCGKDNKKK"
     gene            complement(56941..57165)
                     /gene="pneA1"
                     /locus_tag="ACL8A1_000058"
     CDS             complement(56941..57165)
                     /gene="pneA1"
                     /locus_tag="ACL8A1_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit PneA1"
                     /protein_id="HGS1200921.1"
                     /translation="MTNFNSNEKFCGKSLKSLSADEMSLIYGASDGAEPRWTPTPIIL
                     KSAAASSKVCISAAVSGIGGLVSYNNDCLG"
     gene            complement(57268..57438)
                     /locus_tag="ACL8A1_000059"
     CDS             complement(57268..57438)
                     /locus_tag="ACL8A1_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhrA family quorum-sensing system peptide"
                     /protein_id="HGS1200922.1"
                     /translation="MRKKRGIKKLVTFALLGVFMFSNTIPYQQFIQKNKQLEIRVQSQ
                     KKSNGLDVGKAD"
     gene            57630..58493
                     /locus_tag="ACL8A1_000060"
     CDS             57630..58493
                     /locus_tag="ACL8A1_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS1200923.1"
                     /translation="MNTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEVKSNLSNFKHLSARLLDDRNYDDLEYLYKIEVERS
                     TFLTLEDRTYLEWIKAIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELDTV"
     gene            58876..59496
                     /locus_tag="ACL8A1_000061"
     CDS             58876..59496
                     /locus_tag="ACL8A1_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1200924.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTVFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            59582..60025
                     /gene="tsaE"
                     /locus_tag="ACL8A1_000062"
     CDS             59582..60025
                     /gene="tsaE"
                     /locus_tag="ACL8A1_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HGS1200925.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDTYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            60015..60533
                     /locus_tag="ACL8A1_000063"
     CDS             60015..60533
                     /locus_tag="ACL8A1_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HGS1200926.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQAGGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            60541..61557
                     /locus_tag="ACL8A1_000064"
     CDS             60541..61557
                     /locus_tag="ACL8A1_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HGS1200927.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            61642..62898
                     /locus_tag="ACL8A1_000065"
     CDS             61642..62898
                     /locus_tag="ACL8A1_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HGS1200928.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGASSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQEQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            62953..64119
                     /gene="recA"
                     /locus_tag="ACL8A1_000066"
     CDS             62953..64119
                     /gene="recA"
                     /locus_tag="ACL8A1_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HGS1200929.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVTLDLGDELEIEIEE"
     gene            64426..65796
                     /locus_tag="ACL8A1_000067"
     CDS             64426..65796
                     /locus_tag="ACL8A1_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HGS1200930.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQSSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKQPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSTFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            66172..67128
                     /gene="lytA"
                     /locus_tag="ACL8A1_000068"
     CDS             66172..67128
                     /gene="lytA"
                     /locus_tag="ACL8A1_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HGS1200931.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            67493..67654
                     /locus_tag="ACL8A1_000069"
     CDS             67493..67654
                     /locus_tag="ACL8A1_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS1200932.1"
                     /translation="MNMINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            67641..67847
                     /locus_tag="ACL8A1_000070"
     CDS             67641..67847
                     /locus_tag="ACL8A1_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1200933.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMPLGR"
     gene            67860..68249
                     /locus_tag="ACL8A1_000071"
                     /pseudo
     CDS             67860..68249
                     /locus_tag="ACL8A1_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
     gene            68380..68805
                     /locus_tag="ACL8A1_000072"
     CDS             68380..68805
                     /locus_tag="ACL8A1_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1200934.1"
                     /translation="MTSRVFIDADCISAFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVTKGSAEIVSIDIGTEEYALYRDLTRNYDSNKIIGKGEATSIFLA
                     KKHNGILGSNNLRDVKPYVEEFSLEHMTTGDILIEAFKA"
     gene            69213..69998
                     /locus_tag="ACL8A1_000073"
     CDS             69213..69998
                     /locus_tag="ACL8A1_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1200935.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEIPKELTPSEQLTWIAFLLPDGDIKQIKSVSNLLYGGAYALS
                     HSTFLAETDENTSYVIWQRKFNQLSLPLSQHVLKGKMLEIWKYRPFVSEFWNDFKNNH
                     DKQFVDPISLYLTLKDDDDPRIEEESEALENMILQYLGEDDAS"
     gene            69988..70539
                     /locus_tag="ACL8A1_000074"
     CDS             69988..70539
                     /locus_tag="ACL8A1_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1200936.1"
                     /translation="MPVNTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIYEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFPSMIELFSIL
                     PEYPLKKDGREIPLHFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCGLYSSKI
                     SSNHVSFHLQPQNSVLSSLQLAS"
     gene            70598..70855
                     /locus_tag="ACL8A1_000075"
     CDS             70598..70855
                     /locus_tag="ACL8A1_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1200937.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDITRLTASLLGDEKLSAITSSSV
                     KADIHRFVIELEPVKSTILQNDDISLDQNEIFEILKNFLDG"
     gene            complement(71596..71992)
                     /locus_tag="ACL8A1_000076"
                     /pseudo
     CDS             complement(71596..71992)
                     /locus_tag="ACL8A1_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            72117..72575
                     /locus_tag="ACL8A1_000077"
     CDS             72117..72575
                     /locus_tag="ACL8A1_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HGS1200938.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            72588..73196
                     /locus_tag="ACL8A1_000078"
     CDS             72588..73196
                     /locus_tag="ACL8A1_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1200939.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            73189..73599
                     /locus_tag="ACL8A1_000079"
     CDS             73189..73599
                     /locus_tag="ACL8A1_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1200940.1"
                     /translation="MIKRGDVVVLYLPFPTISSDLAVKNHMYICIDNSMTKNKELVKN
                     QTFKPALLTRRLVKNFMIEEPDLARNPFTRPTLIDLDKVFMLDNTVIPTSYLARRRRN
                     VSEELYEEILDHLVQPRLISLNKSEFMQLNPGTY"
     gene            73610..75025
                     /gene="ply"
                     /locus_tag="ACL8A1_000080"
     CDS             73610..75025
                     /gene="ply"
                     /locus_tag="ACL8A1_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HGS1200941.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGAFLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            75906..76622
                     /locus_tag="ACL8A1_000081"
     CDS             75906..76622
                     /locus_tag="ACL8A1_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGS1200942.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            76746..77217
                     /locus_tag="ACL8A1_000082"
                     /pseudo
     CDS             76746..77217
                     /locus_tag="ACL8A1_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360543.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
     gene            77234..78055
                     /locus_tag="ACL8A1_000083"
                     /pseudo
     CDS             77234..78055
                     /locus_tag="ACL8A1_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601671.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            78110..79093
                     /locus_tag="ACL8A1_000084"
     CDS             78110..79093
                     /locus_tag="ACL8A1_000084"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1200943.1"
                     /translation="MVSAKRINQVLDLHSSIQNPVQVQLTDENFKGQVEFKDVTFRYA
                     ANSEAVIEHVSFKAETGQTVAFIGSTGSGKSTLVNLIPRFYDVSAGEILVDGVNVQDY
                     DFSATAHAGQKVAIVGSTGTGKTTIVNLLMKFYEIDKGSIHIDGVDTKAMTRSEVHDA
                     FSMVLQDTWLFEGTIRDNLIYNQIGISDERMMEASKAVGIHHFIMTLPDGYDTILDDT
                     VTLSVGQKQLLTIARALLKDAPLLILDEATSSVDTRTEELIQKAMDRLMEGRTSFVIA
                     HRLSTIRNADLILVMKDGNIIEQGNYEELMAQGGFYADLYNSQFTEDEAEE"
     gene            79159..79521
                     /locus_tag="ACL8A1_000085"
     CDS             79159..79521
                     /locus_tag="ACL8A1_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 family protein"
                     /protein_id="HGS1200944.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICSVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            79518..80021
                     /locus_tag="ACL8A1_000086"
     CDS             79518..80021
                     /locus_tag="ACL8A1_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HGS1200945.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            80135..80581
                     /locus_tag="ACL8A1_000087"
     CDS             80135..80581
                     /locus_tag="ACL8A1_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HGS1200946.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            80587..81261
                     /locus_tag="ACL8A1_000088"
     CDS             80587..81261
                     /locus_tag="ACL8A1_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1200947.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLTIRGDVAFGKL
                     EIVYVK"
     gene            81422..81613
                     /locus_tag="ACL8A1_000089"
     CDS             81422..81613
                     /locus_tag="ACL8A1_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HGS1200948.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            81620..81700
                     /locus_tag="ACL8A1_000090"
     CDS             81620..81700
                     /locus_tag="ACL8A1_000090"
                     /inference="COORDINATES: protein motif:HMM:NF039231.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1200949.1"
                     /translation="MASSGKMLRNTYTPDSYYVGNLGAWQ"
     gene            81898..82197
                     /locus_tag="ACL8A1_000091"
     CDS             81898..82197
                     /locus_tag="ACL8A1_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HGS1200950.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDLFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFIYENEDLVYEEEVL"
     gene            82194..82511
                     /locus_tag="ACL8A1_000092"
     CDS             82194..82511
                     /locus_tag="ACL8A1_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HGS1200951.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKQQINDFLA
                     GLK"
     gene            82527..83153
                     /gene="ytpR"
                     /locus_tag="ACL8A1_000093"
     CDS             82527..83153
                     /gene="ytpR"
                     /locus_tag="ACL8A1_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HGS1200952.1"
                     /translation="MIFTYNKEYVGDVLMVIVKNSGDAKLDVERKGKVARVFLKDNGE
                     TVAWNIFEVSSLFEIAERGQVFLTDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKTVQIVAGAPNARVGLKTIVALPGAMMPKGNLIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            83186..83947
                     /locus_tag="ACL8A1_000094"
     CDS             83186..83947
                     /locus_tag="ACL8A1_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HGS1200953.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHVINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            complement(84083..85510)
                     /locus_tag="ACL8A1_000095"
     CDS             complement(84083..85510)
                     /locus_tag="ACL8A1_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HGS1200954.1"
                     /translation="MKTTNKVAIYVRVSTTSQVEEGYSIDEQKDKLEAYCKIKDWKIY
                     DVYVDGGFSGANTQRPELERLISDVKRKKVDIVLVYKLDRLSRSQKDTLFLIEDVFAK
                     NDVAFISLQENFDTSTPFGKASIGMLSVFAQLEREQIKERMMLGKEGRAKNGKSMSWT
                     TIPFGYDYSKETGVLSVNPTQALIVNRIFTEYLNGKPVVKIIRDLNAEGHVGRKRPWG
                     ETITKYLLKNETYLGKVKYKDKVYEGQHEPIITQELFDLVQLEVERRQISAYEKYNNP
                     RPFRAKYMLSGLMKCGYCGASLGLRYTRKDKNGISHHKYQCRNRHSKDLEKRCESGWY
                     SKEELERVVIKELERIKFDPKYKNETLAKKEETIKVEEIKKQLERINNQVSKLTELYL
                     DEIITRKELDEKNDKIKTERQFLEEQLENQKSNVLSIRKRKLTRLLKDFDVEKLSYED
                     ASKIVKNIIKEIIVTKDGMSITLDF"
     gene            complement(85678..85878)
                     /locus_tag="ACL8A1_000096"
     CDS             complement(85678..85878)
                     /locus_tag="ACL8A1_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1200955.1"
                     /translation="MSNESRPMEVIKHNLDCKCHRRREWIRVNDKWHAIEFSVDDPNE
                     PPMTEEEKANVALILQQHLSKE"
     gene            complement(85880..86671)
                     /locus_tag="ACL8A1_000097"
     CDS             complement(85880..86671)
                     /locus_tag="ACL8A1_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HGS1200956.1"
                     /translation="MARGRGKLTPQDKEDMKVFSANLNSILSDRNCKQAELSRATGIP
                     PSTLTGYVKGTSLPIPGNVQKIADFFGVPKSVLDPRFITNNFMVNDSSSNTPQIQTIY
                     DELEPPRQGKVLNYAKRQLKEQKNEEETKINEVSEAIRLYSYDYYDHPASAGTGQYLN
                     DVRVERIELPVDVDADFVIPIKGDSMEPDYHDGDLVFIQTSVDLNDGVIGVFNYNGDA
                     YIKQLVIDEDQAYLHSLNPEYKDMPITPDTDFRIIGEVVDLYREK"
     gene            86844..87119
                     /locus_tag="ACL8A1_000098"
     CDS             86844..87119
                     /locus_tag="ACL8A1_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1200957.1"
                     /translation="MNTLNEKAINIFKAVAKETLIQGTYEENFLYSQLEAFCTNCRQF
                     AFGWTELAEEIERQERYLLDSGFTQEEIDDIRFDAAFAGMLDKLNVA"
     gene            complement(87546..87752)
                     /locus_tag="ACL8A1_000099"
     CDS             complement(87546..87752)
                     /locus_tag="ACL8A1_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1200958.1"
                     /translation="MTDYQLEASLIVLGKEFDRTKKNGKESFSVHVSFFDGLDANQHL
                     QEFARQYPVKIDRSNSDQITFLIK"
     gene            87825..88052
                     /locus_tag="ACL8A1_000100"
     CDS             87825..88052
                     /locus_tag="ACL8A1_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS1200959.1"
                     /translation="MPKMTLRAIRTNYNLSAKEVADKLNIHQQTLLKYEHDSSKIPMD
                     LLDKLARLYNVEKDFIFLGKKYELNHNLGEV"
     gene            88137..88457
                     /locus_tag="ACL8A1_000101"
     CDS             88137..88457
                     /locus_tag="ACL8A1_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HGS1200960.1"
                     /translation="MNNAYAQDKRLKATTIGILTVILMNKSDWVVYPDEIARRLGISR
                     RTVDEHFKLLEKAGYLRVYRLGLGRGKGVTVYRFFSDMPISDDYFEYLKTNLEKELST
                     DNGV"
     gene            complement(88885..89109)
                     /locus_tag="ACL8A1_000102"
     CDS             complement(88885..89109)
                     /locus_tag="ACL8A1_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HGS1200961.1"
                     /translation="MGKNQHVVPAKNGGWNVKGAGNSRATVHTTTKNEAKNIARQISR
                     NQGSELIIHGKDGKIQSRDSHGKDPFPPKG"
     gene            89226..89369
                     /locus_tag="ACL8A1_000103"
     CDS             89226..89369
                     /locus_tag="ACL8A1_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1200962.1"
                     /translation="MEDKIIELADYFISESTTYREAKIACEKLLKQVSHEIELRAMES
                     KTV"
     gene            89436..90233
                     /locus_tag="ACL8A1_000104"
     CDS             89436..90233
                     /locus_tag="ACL8A1_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001056933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HGS1200963.1"
                     /translation="MNMNLNTNEILTTTEYDMFRKISNRKITENPKLEEELLSEGQRQ
                     PILVNEKMEVIDGQHRLYYLRKHRKPVRYIIDPTANFKTVISMNTSAVNWALQDYVYS
                     FALEGDPEFVKLAKFLDENELLSDKMVIVAGSGRRDGTAAHVVKKLKKGDYVFSNEKQ
                     LREFCKFYERVLNETKLPNKPFLQSVLWTLYTTSVFDENRMLTQLKKSDLTSEDIEGF
                     AKKKLLLTFLELYNGRWSDDHPSLIQYFINRKGSLTIPSLPKQDEDN"
     gene            90251..90388
                     /locus_tag="ACL8A1_000105"
     CDS             90251..90388
                     /locus_tag="ACL8A1_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1200964.1"
                     /translation="MKVTVYVYGRKLEPDEPIIIPENHRFYDIWNGIVNEMLDNEEDV
                     A"
     gene            90388..90591
                     /locus_tag="ACL8A1_000106"
     CDS             90388..90591
                     /locus_tag="ACL8A1_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1200965.1"
                     /translation="MKLLTKLKLRLEGVLKSVNLDWREVAVELMNDLFEERKRRFAFE
                     QENYDLKQQLAIYKEKEQGEQYV"
     gene            90584..90838
                     /locus_tag="ACL8A1_000107"
     CDS             90584..90838
                     /locus_tag="ACL8A1_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1200966.1"
                     /translation="MFKALKTIKKIKQLQKEMHDASVAFLLMQDLGLVPDSEKGRTIA
                     KSFYDVSHMLKDVLGGKSVDEAMKRLNSEVKIEDVEQEDD"
     gene            91143..91328
                     /locus_tag="ACL8A1_000108"
     CDS             91143..91328
                     /locus_tag="ACL8A1_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1200967.1"
                     /translation="MKKRLYYKKWKQELREAMREEIDGDYLTEKMVRKMSISDMSHYL
                     NQLALEEAGYCGTMFNY"
     gene            91346..92098
                     /gene="bet"
                     /locus_tag="ACL8A1_000109"
     CDS             91346..92098
                     /gene="bet"
                     /locus_tag="ACL8A1_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HGS1200968.1"
                     /translation="MTNNQLATQTKRNITTDPSLLTGADIKKYFDPQNLLTEKQVGQA
                     LALCKGRNLNPFANEVYIVAYTNRNGGKEFSLIVSKEAFLKRAAQCKDYEGFEAGVVV
                     VDSEGVMHERKGAIMLPEDILIGGWARVHRKNFKVPVEIFVSREEYDKKQSTWNTMPA
                     TMIRKVALVNALREAFPEDLGNMYTEDDGGETFDRIKDVTPQESREDVVARKMAEIEQ
                     FNKEQEANHADPEPAQTEETIQGELLDGELEY"
     gene            92108..93172
                     /locus_tag="ACL8A1_000110"
     CDS             92108..93172
                     /locus_tag="ACL8A1_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001157031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1351 domain-containing protein"
                     /protein_id="HGS1200969.1"
                     /translation="MQELQVIDDKKINKIYEMITTDELTRESFEKDLIEATEKYKDYI
                     PTAGTLKDDKAKRAEFNKLIESKNRIRIDTKNLLSETANTWDSYAKSIIDPFATVVSE
                     FDKGIKEIEEHQRQLKIDTVKSYLANKSAEYMLDPRLFDEKALEYVKASDFMADGVTL
                     KKATMKSLDDMVTFEFQKQQEFEKAKSAISGLCAEYGMTDSPYIRQLKDLTIAEVFGQ
                     IKADYEFEKQKEEIRQAQERAERESQELLAAQQTKQQEQAPKSTETPNFDPETGKILD
                     GGQILQNEPNALRGAENDLKRYAQKMTLEVYFVDTAEKDCFKAGLSQLGFDFKKNYQV
                     RGYQRIEPLTQAELNEQCGW"
     gene            93175..93336
                     /locus_tag="ACL8A1_000111"
     CDS             93175..93336
                     /locus_tag="ACL8A1_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1200970.1"
                     /translation="MTEIEKISEELAEYGVPDELIGKIEDLLATLYGEKKEIGDRKIL
                     DISPESMGR"
     gene            93339..93662
                     /locus_tag="ACL8A1_000112"
     CDS             93339..93662
                     /locus_tag="ACL8A1_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1200971.1"
                     /translation="MDIRKISDSVAIYSDGKKLQVIHNLGDEFILDFNVGEDSVWNLN
                     GQVVEIIDMIEPVFKVFSFCSKSGEGMQRLKHAIVHFEIFEQYIRDNQEDLMIWWHNP
                     GGEYD"
     gene            93655..93990
                     /locus_tag="ACL8A1_000113"
     CDS             93655..93990
                     /locus_tag="ACL8A1_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein Cse60"
                     /protein_id="HGS1200972.1"
                     /translation="MIKTVFFSCDYPHHEAIDDQINSWLAENPGIKLIDIKFQSNVSA
                     VADSGVSAEYWHTSALIIYKVPSENNISSINSNGLGFIISCEKCGSLSIIKGKDVGQN
                     VCYECKGEK"
     gene            94085..94501
                     /gene="ssb"
                     /locus_tag="ACL8A1_000114"
     CDS             94085..94501
                     /gene="ssb"
                     /locus_tag="ACL8A1_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGS1200973.1"
                     /translation="MINNVTLVGRLVAPPDLRKTPNNVSSLQGTLAVNRNFKNENGER
                     EADFINFQAWRGTADIIAQYCSKGSLIGLTGRLQVRSYEKDGQRRYVTEVIAESVALL
                     ESRNSQHGQGNSFQNGNSSPFTDPNPFDLPNDGLPF"
     gene            94511..95017
                     /locus_tag="ACL8A1_000115"
     CDS             94511..95017
                     /locus_tag="ACL8A1_000115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGS1200974.1"
                     /translation="MSKIKILDACCGSRMFWFDKNESHTIFMDIRQETFEIHDKKVNV
                     DPDIIGDFRDMSFEDNTFNLVVFDPPHLKWAGPNSIMKAQYGQLDKVTWSEDLAKGFE
                     ECLRVLKVGGTLVFKWSDRQINVKKLLEVIPFKPLFGQQRGTTHWLTFVKFEEDKNGV
                     DGLGGLET"
     gene            94983..95192
                     /locus_tag="ACL8A1_000116"
     CDS             94983..95192
                     /locus_tag="ACL8A1_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1200975.1"
                     /translation="MEWTDWVDWKPETKTDIKTKIENDGYTFPHYDKKNNGVKYVIST
                     MDIKQDCLRLGVPFEDMYPLQTTLF"
     gene            95206..95535
                     /locus_tag="ACL8A1_000117"
     CDS             95206..95535
                     /locus_tag="ACL8A1_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1200976.1"
                     /translation="MASKINVTERIAIIIEKQKIEVVTTLNYDMSISFDNKDTAPTLD
                     DNGDLFEPVYKCKVKAIPKNDVFFTSLIRVKSNIKTLQEVKKFFEFVNENRENLFEMA
                     GFKGALE"
     gene            95532..95963
                     /locus_tag="ACL8A1_000118"
     CDS             95532..95963
                     /locus_tag="ACL8A1_000118"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0000400 - four-way junction DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HGS1200977.1"
                     /translation="MKLTLNIEPKPQSRPRFARRGSFTTTYEDKDMKTWRNHCQLLIA
                     NQYMGQPILEGALRARLRFYIKPPQYISKVKKNQQTLLDEIIPVGKKPDIDNYEKALY
                     DSMSGIVFQDDGQIALHDVGKFYSLNPRIEVEIEVMKSLSI"
     gene            95979..96161
                     /locus_tag="ACL8A1_000119"
     CDS             95979..96161
                     /locus_tag="ACL8A1_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1200978.1"
                     /translation="MADYALYQGDVFVTLGTLAQISSETGITERMLKYYTYTSHQRRH
                     PNGRAVIKIEEEDNEN"
     gene            96186..96503
                     /locus_tag="ACL8A1_000120"
     CDS             96186..96503
                     /locus_tag="ACL8A1_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1200979.1"
                     /translation="MMEELKQKVNEVYNWTVEDGKPQPPKQDLPQAVKDRADYFWEMA
                     EDGMTFMGAMECIFADEKPTDYDLGATKDWLPKSKEFDDWVGYSPGMSQVVIAVYLIY
                     RGN"
     gene            96505..97200
                     /locus_tag="ACL8A1_000121"
     CDS             96505..97200
                     /locus_tag="ACL8A1_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HGS1200980.1"
                     /translation="MNKQELIKKLEERRTITGNFQGYVVWWKDVKEIFEQLDESEIGH
                     ADEAPRYVKNILARLRELPLHDREVWLKAIMSEFEQDFSRAKWREGYEQGKIEGMVER
                     EKVIVPQCVAEYIEFKKKNNFHVYGAMRVIEDHYDKKVPDWFYENNIEKFCLAWLDGY
                     EVEKEKRYFVKIKGNIKENMLVYGELLKRYFFTKSFSLDDVIYSHTRKELEDAKFGWV
                     FDCPGIEIEEVIE"
     gene            97197..97457
                     /locus_tag="ACL8A1_000122"
     CDS             97197..97457
                     /locus_tag="ACL8A1_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015971722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1372 family protein"
                     /protein_id="HGS1200981.1"
                     /translation="MKRFIAIWIVLSATLNIWQCVHIKNLEKKRPIVIYKADNKGAEI
                     KGRVVHKEKIGELYTITIQNYGTFVVSQDNYEFLKIGDEVRL"
     gene            97454..97867
                     /locus_tag="ACL8A1_000123"
     CDS             97454..97867
                     /locus_tag="ACL8A1_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HGS1200982.1"
                     /translation="MIPKYRAYDSGSLSRMYQPDEVMVGDGNIWIIDEDSVAGDWIVN
                     NDIHLMQSTGLFDRNGKEIFEGDIVKRYRSPFFKAEWEYRIEIVIKEKASLLLGRRFD
                     KALCARSLDSPFTKSDLLEVIGNIYENPEVLEVTE"
     gene            97864..98106
                     /locus_tag="ACL8A1_000124"
     CDS             97864..98106
                     /locus_tag="ACL8A1_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015971724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HGS1200983.1"
                     /translation="MNPEKIDNVNKPSHYQGRFGMESIDALRNFMTDEQLKGFYLGNA
                     LKYLLRHQKKNGLEDLKKARKNLDWLIEEMENKNET"
     gene            98096..98356
                     /locus_tag="ACL8A1_000125"
     CDS             98096..98356
                     /locus_tag="ACL8A1_000125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1200984.1"
                     /translation="MRPKKYPYTGSKINKVTTTGIGAQELVVFPNVAFRKDLLKHIFS
                     VVKQHDNATIIYFRIPKVFGYEEERAKVYLSYEKTMMILNSY"
     gene            98432..98854
                     /locus_tag="ACL8A1_000126"
     CDS             98432..98854
                     /locus_tag="ACL8A1_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HGS1200985.1"
                     /translation="MNKAKELLKELQNLDMDIQSRIDEINELEAGLLSSPKWIDVKVQ
                     GGQARKVDDVYTQLVVMKEAIEQDTKEVINRKLELGRLINKLKNPKSRSILRVTYITK
                     MYVDDICDKMEISRTTFYTWRNMAISELNEVLERMELN"
     gene            99309..99809
                     /locus_tag="ACL8A1_000127"
     CDS             99309..99809
                     /locus_tag="ACL8A1_000127"
                     /inference="COORDINATES: protein motif:HMM:NF015550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0051276 - chromosome organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HGS1200986.1"
                     /translation="MVKKNRNKRSREQVDEFGLNSRERNFADEYIANGNNATQAYLKI
                     YPTSGEANAANKGSAWVRKGEILNYIKIKTKERLDASGLKANDIIDRLIDIAFGRPIV
                     GYSKQTDKITGEVIKYIEYEGTAPIDEQIKALELLGKYLKLFTDKVEADVSGTVVFAN
                     ESDIPD"
     gene            99787..101085
                     /locus_tag="ACL8A1_000128"
     CDS             99787..101085
                     /locus_tag="ACL8A1_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HGS1200987.1"
                     /translation="MSQTYQIKQSDIVIDLPKTVGAGYGQFWRSRSLYRVVKGSRGSK
                     KSKTTALNYVIRLLKYPWANLLVIRRYSNTNKQSTYTDFKWACNVLGVTHLFKFNESL
                     PEITVKATGQKILFRGLDDELKITSITVDIGSLCWAWFEEAYQIETEDKFSTVVESIR
                     GSLDVPDFFKQITVTFNPWNERHWLKRVFFDEETRRADTFATTTTYKCNEWLDGVDIK
                     RYEDLYHTNPRRARIVCDGEWGVAEGLIYENVTVKDFDKDELLRDSANKLCIGLDFGF
                     THDPTALCCSLINDTTKEIYVFDEAYKVGLITKEVAKMIKDKGYHRSQIIADSAESRL
                     IEELRSEHGISRIKESRKGKDSIMAGVSKLQGYAIYVHPDCKNIMDEFYSYCYQRDKE
                     GNWLNKPEDKNNHLMDALRYSLQCIEGGKATVRRRSDYGL"
     gene            101097..102566
                     /locus_tag="ACL8A1_000129"
     CDS             101097..102566
                     /locus_tag="ACL8A1_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046798 - viral portal complex [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0051027 - DNA transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGS1200988.1"
                     /translation="MYQYLTYPRDGYDEGSLKKDLIYKLITIHSTEGSHLKKLKSYYL
                     GEHAILEHTRRNVNAPNYKTVANHAKDIADTATGYFMGNPIKYNNTADGDIDELLTAF
                     DGAEIDQVDAQNALNMAIYGRAYEYIYAKEGMAELDSTSIDPENTFMVYDDSIERKPL
                     FAVYYYEVKDDTKDTTKHQAEVFTENLHYHMVLRSTDSGTTQSEEATPHNLGQIPIIE
                     YRNNHFAIGDYEQQISLIDAYNSLMGNRVNDKEQAVESILVLYGTQLADTPEDAKVAM
                     KILSEEGLLELPGDSARAEFLKNTLDESATEILRTALKEDIYTFSHVPNLTDENFAGN
                     TSGVAMEFKLMGLEMITKTKEANYKRGLRQRIAIFAHYLGMKQIALESHSIVPQFSRG
                     LPKNLLEISQIVNNLEGKVTNRQLISLLPFVEDPDAELEALEEEKKKNMEDMPMFNKD
                     NTKPEDEVEDEESGVLGEEESQSDLPADGQGRKAGRPVR"
     gene            102475..104112
                     /locus_tag="ACL8A1_000130"
     CDS             102475..104112
                     /locus_tag="ACL8A1_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HGS1200989.1"
                     /translation="MKNQEYWAKRKANLIYQQMDKAEKQADQFDKVYQEAKTYLDKEV
                     NKIFDKFQRDYGLSQVEARQVLKNMKDKKNLNELRKVLEARPNDPNIQRLLADLDSPA
                     YSFRMKRLERLSDDLDRMRESIYHSEKTGSDAFYSDLMKDSYYKATFDLQQQTGLAYG
                     FSGLPESEIKHLQSFSWVGDGSTYSTDIWKNTGKLTSSIKDELLMSLMTGRDTRETAQ
                     AIAERFNVGQNDARRLVRTESAFFHNQMELLSYEEADIEKYIFAAVLDKRTSRICQEH
                     DNQVYDRDKAVPGVNCPPMHPWCRSTTVGYDEDADYSKLKRRARNPETGKTELVPADM
                     TYKEWYSKYVAKDGEKVYNQDTREAKAKFYSEQLLSKISGVEPKITSDMQRIAGENKL
                     AGLEFRKKTVESLSRKIIADSLVENISLSKAVSKINDALRYTTIFDSDTFTEEYLKMK
                     QKLIAEGYKIVKVKNTWLVDGPYKGVNTVVEKDGINFEMQYHTQESFDLKNGSLHELY
                     EKYRDTNTSDLERMKLFKEMLDLSNGLEIPKNIERVK"
     gene            104114..104323
                     /locus_tag="ACL8A1_000131"
     CDS             104114..104323
                     /locus_tag="ACL8A1_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1200990.1"
                     /translation="MKDIKYYRTTTNNAQVLRLIDGVMQVFDIEKKWVNSMDWFNKIF
                     FNDFTDFEEISENDAFTYIDRMVAA"
     gene            104320..104733
                     /locus_tag="ACL8A1_000132"
     CDS             104320..104733
                     /locus_tag="ACL8A1_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HGS1200991.1"
                     /translation="MIDIALAIAKKAHAGQVDKAGVDYIQHPLYVASQVNTEQEKAVA
                     LLHDVIEDSDITAADLFASGLSNEVVTAVQILTKKKGQSYQEYLGKVKSNNLARVVKL
                     ADLKHNSDLSRLKSVTNTDYERVKKYKNAIYYLST"
     gene            104776..105039
                     /locus_tag="ACL8A1_000133"
     CDS             104776..105039
                     /locus_tag="ACL8A1_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1200992.1"
                     /translation="MKYRKKPVVVEAVQFLDTEESIDELCDFGLDPVRIDYADLKNPL
                     LKIETLEGLMVATEGDYIIKGVQGEYYPCKPDIFAETYEKTEE"
     gene            105041..105292
                     /locus_tag="ACL8A1_000134"
     CDS             105041..105292
                     /locus_tag="ACL8A1_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6275 family protein"
                     /protein_id="HGS1200993.1"
                     /translation="MLEKAKQLASQEFLRLSGREIKAEDCFVVWFSKTLQNWKALVST
                     NAITSSEPCGDYAEITHNGDKNETYVDVYAKVSNRAIKD"
     gene            105519..106103
                     /locus_tag="ACL8A1_000135"
     CDS             105519..106103
                     /locus_tag="ACL8A1_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HGS1200994.1"
                     /translation="MSEEINATVSTESTETVDTQGNVDTVQEEKHERTFTRAEIGKML
                     SAERSKWEAEQEAKENEAKKLAKMNADEKQKYQLDQREQELADREKAIARKELTAEAK
                     AMLSERDLPVELVNVVDLTSAETVSQSVAVLQKSWEQAVQKGVQEKLKGGAPMKQAPV
                     DSDGITKEEFARMGYQSRNELYQKNPELYKKLKG"
     gene            106109..106951
                     /locus_tag="ACL8A1_000136"
     CDS             106109..106951
                     /locus_tag="ACL8A1_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HGS1200995.1"
                     /translation="MTAGQTKLATMVNPEVMADMVSAKLPKLIKFTPLAYVETALQGQ
                     PGNTLTVPAWEYAGDATEVGEGQAISPDQLTTKKTTMTIKKAAKGYEITDEALLSGLG
                     DPLGQATYQLGLAIANKIDDDLVAVAKTATQHITETPTTLAAIDKALEIFEDEEDAQY
                     VAIINPKDAIKLKTDVAKEWTKGSELGADMVVSGTFGEVAGVQIVRSKKVDEGKGFIV
                     KVSPSQTQTDDANKYGAFVIMLKRDVAIETDRDILKKTTVITGDEHYGVYLYDPTRVV
                     KFGE"
     gene            106963..107145
                     /locus_tag="ACL8A1_000137"
     CDS             106963..107145
                     /locus_tag="ACL8A1_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HGS1200996.1"
                     /translation="MSLLLRRHYIQEEQVSQYSDLENKTLEELKNLAKEAGIAGAYKL
                     SKAEIVEVLEDLKSEI"
     gene            107135..107323
                     /locus_tag="ACL8A1_000138"
     CDS             107135..107323
                     /locus_tag="ACL8A1_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1200997.1"
                     /translation="MKFKIKQDFYDWESNVKRLAGGELEITEERYVELADNIASNGVA
                     ISDVLEKILPEPEFLEED"
     gene            107325..107636
                     /locus_tag="ACL8A1_000139"
     CDS             107325..107636
                     /locus_tag="ACL8A1_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HGS1200998.1"
                     /translation="MSIELLKKLTGEEDTQLLMLLQTRATNLILSETNRTSLTPALSL
                     LIPEVAIELHNRSGAEGERSRTEGGIEVVYGENGLSTGLLQRIRMHRLARVAGHVFEA
                     E"
     gene            107617..107955
                     /locus_tag="ACL8A1_000140"
     CDS             107617..107955
                     /locus_tag="ACL8A1_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1200999.1"
                     /translation="MFLKQSRLKPYPMRRFEKTVTEEGVAKEGYVKEAETIRLELWPA
                     SSKLQSELYGERVNDILNANANKSATIKVKDGVCIDSQTEVTHRVISKKVYTHHQVLE
                     LERVRATRGR"
     gene            107930..108445
                     /locus_tag="ACL8A1_000141"
     CDS             107930..108445
                     /locus_tag="ACL8A1_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HGS1201000.1"
                     /translation="MSGLLGADRLIAKCRRLASKKTGEDIVLRAVHNATIKVVQADAR
                     RLAPARDGELITSIKTRAKMDGDKAIGEVYTNLKYAPYVEFGTGPIGQASHSGISPEV
                     SVTYKSSPWYVHEDQINVGPYHFQKIGEFYKMYGQPAQPYLYPALRDNQERVSKNISN
                     YVRRKIREQIK"
     gene            108442..108810
                     /locus_tag="ACL8A1_000142"
     CDS             108442..108810
                     /locus_tag="ACL8A1_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201001.1"
                     /translation="MINIKPVIYKELQKVADNVTDTYPSDWENFPVVIFLEEQNKPGD
                     WFDDQEQKSSIRYKVDIFDDTSTSELAVKINQIFESLGLRRTDCQDVPDPSHLRHKVM
                     RFEGVVDLDSELVFQFRMEN"
     gene            108813..109226
                     /locus_tag="ACL8A1_000143"
     CDS             108813..109226
                     /locus_tag="ACL8A1_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HGS1201002.1"
                     /translation="MLANGITLSYSKTKGSYTKLVGLKEVPEFGIEPEKVENTTLEDK
                     VKKYEFGIGDAGELEYKFSYKNDSATAPYRVLRKAADDKEKLYFEQAYPDGTKVTFEG
                     QVSVKLGGGGVNAVIDFTLKIALQSELEFTDGVGG"
     gene            109231..109611
                     /locus_tag="ACL8A1_000144"
     CDS             109231..109611
                     /locus_tag="ACL8A1_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6096 family protein"
                     /protein_id="HGS1201003.1"
                     /translation="MALKYTTWKVTDEKELKLRLTSHQAATVEEKIGMNLLKIFMPEA
                     GEEFTLPPLKVMLLLVHGALQQYEHGYSFEDVYDLYDEYVDNGGDQTTFMTEVLMPLF
                     EVSGFTPRGSKNKKTSKKKMTVVE"
     gene            109650..110000
                     /locus_tag="ACL8A1_000145"
     CDS             109650..110000
                     /locus_tag="ACL8A1_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201004.1"
                     /translation="MFLDIGGKPLDFWDLTVLEIREMIESYNRVKTQERKEKIIDSYR
                     LSQMISNHVSLLLSNDAKIVEFWEYAPELFVEEQQAVELERQKQALLLHKERMREFAE
                     RHNRKRKEEVNGNS"
     gene            109987..113640
                     /locus_tag="ACL8A1_000146"
     CDS             109987..113640
                     /locus_tag="ACL8A1_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201005.1"
                     /translation="MATLDELKVMIDAEIAPFRKKMKEVENQVKGTSDQVKNATAKVR
                     EQSNSIGSAFGKLAKFAGFAILGKKLLDVGMYSTQTALEVSASMNQIKRQMGESSQSF
                     LKWVNDNANAMNMGVGEATNYGAVYSNLFSGFIKDTNKLSAYTAKMLQTSAVVAEGSG
                     RTITDVMERIRSGLLGNTEAIEDLGINVNVAMIESTEAFKKFANGQSWQQLDYQTQQQ
                     IRLMAILEQATAKYGNTLSNSVNGRISLFKSLMKDAALNLGNSMLPIINAIMPVLNSF
                     AMVLKNVTAKLAEFIALMFNKKATVKDGVGGAVGDMGNAMKDVAGGAGDLADAVDDAG
                     DSAGGLADNLGDSAKNAKKAAKELLGLLGFDEINILQKPKDDDAGGSGGGGKGGKGKG
                     GGGGPFKDILPEVELTDMDNKFKSIFDGLGDKLKGLFDLFKKGFDAAFRPEGIKRIKT
                     ALDQIAKTMGEIATDPRVVNAFNRMAEKIAYALGQVTGSITTIGLGIGVFLAESIANG
                     LGRQKERITRALVALFDNVGNLSEAVGNIAQDFSSAFYDVITSTGAVRIGSAIVSTLL
                     SLTSTIVEVGSKLAGSLFKGFEKFVVTSAPKISSVFQSLLDTVAPVFESIERSVNKFG
                     DGLSRVYDEHVVPTINSIANAFNGLIDIIQILWENSWQPFAEFLSGVFGVSIEGISDL
                     LGGGLLATLGLLADAIKLVADGFTVFSDWCKENKEPIVALITTWQTINFLSWAEQAGG
                     LAGAFSLLGSKVSSIVGGIKNLGLAIKALTFDKLVSFGETIYLNTLYAKDFVVNSGKT
                     IAQLGKTALELGKSALAWTAHAAKMGLATAAKFAHSVATGVATAATWAFNAALAVLTS
                     PITWIIAAIAALIAIGVLLYQNWDTVVEFAKTAWQGLCDFISGICQAIGEFFSGLWTK
                     LQEIFEPIGQWFGEKFQQAWDAIVNIFSGIGEWFSGVFQGAWDAIVNIFTPIGSWFGQ
                     RWADVTSALANIGAWFTDMFQKAWTGLTNIFSKLGSWFGERWADVTNALSSVSNWFGE
                     MFTNAYNAVKDAFSSIGDFFKGVWDTVKSIFVNAGQMVGEAVGGAFKSAVNAVLGTIE
                     NVVNGFIGMINGVLGVVRNLPGLGWVGSVSTVSLPRLARGGIVDSPTIAMIGEAGKEA
                     VVPLENTGFIQTLGRVVSSAVVNAMAGVSPQGGFSGDGDIVIQIAGHEFGRVAIQEIN
                     KEHERAGQTLLKI"
     gene            113649..113999
                     /locus_tag="ACL8A1_000147"
     CDS             113649..113999
                     /locus_tag="ACL8A1_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6711 family protein"
                     /protein_id="HGS1201006.1"
                     /translation="MAQLTINGVAVKPPKYFQVGIQDIDGETGRNANGDMMRDRITTK
                     RKLDCEWGMMTQGELSQLLHAVSSEFFEVSYPDPMDGQVTKTFYVGDRTAPSYTFTEK
                     FKPWSGAKFNLVER"
     gene            114004..>115268
                     /locus_tag="ACL8A1_000148"
     CDS             114004..>115268
                     /locus_tag="ACL8A1_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HGS1201007.1"
                     /translation="MDALTRRQFDRAMFAKERTLAIRVGDYASRDIKEASFEYGYIKG
                     DTYKPGGTCAGSGKITFTSIITTFNKLDTLHPEIGLLVGDTYQWVKMGEYFINDIEID
                     RNRNTTTLELMDGMFKLNREYVTDLHFPAEVREVIQEICLKTGIELANDYFGISAMRY
                     HIEQVPEGKKLSFRDMLSAMTQMIGMSCFFNREGKMEIRDLTESNITINADSYFLHGL
                     TKSEIEYQIAGITCKTDKKSLTVGMKTGRSLELDNVFMTQSALNDLYYKLKNLTYYPY
                     NLNYQGHLLLEVGQWVTIQTNKKETFKVPVLSQSFTFKGGLRGRISADSKAGNDTQYS
                     YEGTITKQIKQQDGVEAKIQAQIEAADKDFDQKVDKIKKDFNDQVELAKARAEEVKRE
                     LSDTINQRFNSFDNGPLKETKRKAEEALR"
BASE COUNT        35558 a        20732 c        25136 g        33842 t
ORIGIN      
        1 atttaaggcc tactgttata gattcaatac actatatatg tgtttgtctg ataaaaattt
       61 ttactctttt tgattttaaa taagtattag tttacattat ggtgtgaatt gggtttgata
      121 tctcttttga ggaagttgcc ttagattttt ctgattgtgt tttattgtat agtgtatctt
      181 gcttgttttg aacagaattt tttatgacat ttgtcatatt ttctagtgac agaagcttct
      241 gtctcctctg attttaaaag actataattg tagtatgaaa tgggggaaga agagatgaga
      301 aataaaatga ttatcgcagt aagtttagta gtagcaggag ttatgaccta tctcatgttt
      361 tcgggattgg atgaggattt ctaccatttt ccttggaagg tctttgctgg ctttggaatc
      421 atgtcttggc ttgttagaga aggtttgaaa ttagtcagag atgtgaaaaa ggagtttgag
      481 gaatgaagag agggatcatc tatttcttta tcggcctgtc actcttggta tggttagtgg
      541 aaatgtttac tgattggttt gatcaagcct tgcttcgcca attcattcgt ggtgctttgg
      601 ggtttggatt tatgattttc gtcgttttcc ttatgggaat ggagtggttg aaaggagagt
      661 ctcatgacgg tggttaaagt tgagaaattg agtaagaaaa taaaagacaa ggaaattttg
      721 cggaatatct cctttgaaat caacgatggt gaatgtgttg ccttaattgg tccaaatggt
      781 gccggaaaga caacactctt agattgtttg cttggagata aactggtcac aagcggtcaa
      841 gtatctattc aaggtttacc agtgacgagt tctaagttag actatactag ggcttacctc
      901 cctcaagaaa atgtaatcgt tcagaaatta aaagttaaag agttgattgc tttctttcaa
      961 cgtatctatc caaatccttt gagcaatcag gaaattgatc aactattgca gtttgtcaag
     1021 caacaaaaag aacagttggc agaaaaatta tcaggcggtc aaaagcgtct cttttctttc
     1081 gtcttgacct taattgggcg accaaagatt gtttttttag atgagcctac tgcgtccatg
     1141 gatacctcaa ctcgtcaacg tttttgggaa attgtccagg agttaaaagc gcaaggagtc
     1201 accattctct attcgtccca ttatattgag gaagtggaac acactgcaga ccgcattttg
     1261 ctcttaaata agggagagtt gattcgagat acgacgcctc tagctatgcg tagcgaggaa
     1321 atagaaaagc actttatcct tcctatagct tacaaggaag tcgtagagca gtcaaatttg
     1381 gttgaaaact ggaccctaaa gcaagattct ttacaagtag tcactcgaga agcagatgct
     1441 ttctgggaac tattagccca agcaggatgt aggatgcaag aaatcgaagt taataatcgt
     1501 agtttgttga atacaatctt tgaagagacg cagaagggag ataactaaga tgaaacgatg
     1561 gatagcactt agtaagatag aatttttatt gaccaaacga caattaatct attatctatt
     1621 gtcggtaggg atgccgacgg ctttctattt atttttttct ggtatctatc aggacacacc
     1681 aggtgaactc gcgaatttta tgcgtgatta cctcatttcc atgactgcct tttccatgat
     1741 gtcgacaact atcctctcat ttccagctgt tttacatacc gataagatga acaactggca
     1801 gaaaacattg cgtcatagcc ctgtaaatat ggtagaatat tatctatcaa agataacaag
     1861 tatgctggtt gattatttgg tctcaattct ggttgttttt acagttggac attttgtaag
     1921 aggtgtagac atgcctctag taaaatgggt tggagctgcg attttgctga ttgtggggag
     1981 tattgctttt gtagcacttg gcttgacctt gactctctta ccaactagtc agctgatgtc
     2041 tgttgtgggc aatcttcttt atctaggatt ggctgtttta ggcggactct ggatgcccat
     2101 ttctttattt ccagactgga tgcaagcagt tggtaagtgt ctcccgacct atcaattgat
     2161 ggagttgctc aagaccttct taaacgaggg tggcatcaat ctatcagcca cagtttattt
     2221 acttgttttt tcagcagttt tatttggttt gactatttac cttcaaggtc ataaggagaa
     2281 tgcttaatgc ttgaaagact gaaaagcata cactatatgt tttgggccag tttgattttt
     2341 atgctttttc ccatcctacc tgtagtgatt ggggagcttc ctgcttggca tttattggtt
     2401 gatattctat ttgtagtgac gtatttgggt gttttaataa ctaagaacca gcgcctatct
     2461 tggcttttct gggggctcat gctggtctat gtagctggga ataccgcctt tgttgctggg
     2521 aattatatct ggtttttctt tttcctttcc aatctcttaa tttatcattt tggcgtacgt
     2581 agtttaaagt ctttacatgt atggacattt ctccttgctc aagtccttgt tgtggggcga
     2641 cttttgattt ttcagagaat cgaagttgag tttctagtct atatgcttgt aattcttact
     2701 tttgtcgatt tgatgacttt gggctcggtt cgaattcgcc ttgtcgagga tttgaaagaa
     2761 gctcaggtca agcaaaatac ccagataaat ctattgctag cagaaaatga gcgcaatcgt
     2821 atcggtcagg atttgcatga tagtctggga catacctttg ctatgcttag tgtcaagata
     2881 gatctagcct tgcagctatt tcaaatgcag gcttatccac aagtggaaaa ggaattaaga
     2941 gaaatacagc aaattagcaa agaatcaatg cgtgaagttc gaaccattgt ggaaaatctt
     3001 aagtctagaa ctttgacatc cgaactagag actgtgaaaa agatgttaga aattgctgga
     3061 attgaggtgg aaacagataa ccaactagat actgctagcc ttactcaaga attggagtca
     3121 atggcttcta tgattttgct tgagttggtg accaatatca tcaaacatgc caaagcgtct
     3181 aaagcttact taaaattaga acggacagag aaggaactca ttttaacagt aagtgatgat
     3241 ggctgcggct ttgcttttct aaaaggagat gagctccata caatccgaga tcgtgttttt
     3301 ccattttcag gagaagtaag tgtaatcagt cagaaacatc caacggaagt gcaagttcga
     3361 ctaccttata aggagagaaa ctaagatgaa agtattagtc gcagaagatc aaagtatgtt
     3421 gcgagatgcc atgtgtcaat tgctcacgct tcaaccagat gtggagtctg tccttcaagc
     3481 caagaatggg caagaagcaa tccaactatt agaaaaggag tctgtagata tcgccatcct
     3541 tgacgtagaa atgcctgtta agacaggtct tgaagtcttg gagtggatac gatcagaaag
     3601 gcttgaaaca aaggtggttg tggtgacgac cttcaagcgt gctgggtatt ttgaacgtgc
     3661 ggtcaaggct ggagtggatg cttatgtatt aaaggaacga agcgttgcag acctcatgca
     3721 aaccttgcac accgtcctcg aaggacgcaa ggagtattcg cctgaattga tggaagtggt
     3781 gatgacccgt cccaatccgt tgacagaaca agagattgct gtcttaaagg ggatcgcccg
     3841 gggcttatcc aaccaagaaa tcgcagatca gctttacctc tcaaacggaa ctattcgaaa
     3901 ctatgtcacc aatattcttt caaaattgga tgctggtaat cgaacagagg cagctaatat
     3961 cgcaaaagaa tctggttggt tatgatgata taatattttc atgaagacta tgtgctaaaa
     4021 ttgacggtag tgaatggaac aatacctata gtagattgaa actagaatag tacacctcta
     4081 cttctaaaac attgttagaa atcgatttga ctgtcctgat cgatttatcc tgttcttatt
     4141 tcattttact ataactttag gagggcttag tttcctccgt tttgtttagt ctaaggtggt
     4201 agcaaggagc taaacctagg ttttctatga aattttttca atcaatcttc aaaaaacact
     4261 tgaaagtgtt tacaattagt gatagaatgg aataagtaga aatatgaaaa cgaaattttc
     4321 acatcttcac ttttgtaagt agatatagag cttcaatcag tacctctact tgtaaaagaa
     4381 tgtgtgagat agaaaggaca ggaaaaggaa tgaatgcaga tgatacagta accatttatg
     4441 atgtcgctcg tgaagcaggt gtttccatgg cgacggtcag ccgtgtggtc aatggcaata
     4501 aaaatgtaaa agagaatacc cgtaaaaaag tgctagaggt aattgatcgc ttggattatc
     4561 gtccaaatgc agttgcgcgt ggtcttgcaa gtaaaaagac aaccactgtc ggtgtcgtga
     4621 ttccaaatat taccaatggt tatttttcga gtttggctaa ggggattgat gatattgcag
     4681 aaatgtacaa gtacaatatt gtcctagcta atagcgatga agataacgag aaagaagttt
     4741 ctgttgtcaa taccctcttt tcaaagcagg tagatggcat tatctatatg ggttatcact
     4801 tgacagataa aattcgctca gaattttcgc gttcacgtac tccgattgtt ctcgcaggaa
     4861 ctgtcgatgt tgagcaccag ttgccaagtg tcaatattga ctataagcaa gcaacaattg
     4921 atgcagtgag ttaccttgct aaagaaaatg agcgtattgc tttcgttagc ggtccgttag
     4981 tggatgacat caatggtaag gttcgtttag ttggctacaa ggaaaccttg aaaaaagcag
     5041 gaatcgctta tagtgagggt ttggtatttg aatctaaata tagctatgat gatggttacg
     5101 ccttagcaga gcgtttgatt tcatcaaatg caactgcagc agttgtgaca ggtgatgagt
     5161 tggcagcagg agtcttgaac ggtttggctg ataagggtgt ttctgtgcca gaagattttg
     5221 aaattattac tagtgatgat tcacaaatct cacgctttac ccgtccaaac ttgacaacga
     5281 ttgcccaacc tctttatgac cttggtgcca ttagtatgcg tatgttgacc aagattatgc
     5341 ataaggaaga gttggaagaa cgtgaagttc tcttacctca tggtttgaca gaacgtagct
     5401 caacacgaaa acgtaaatag aaaaaatcag ggaatcgaga agatttcctg attttgtgct
     5461 cttaaacaag agatgttgac ttaatgaata ccatagagcg gactaggcgg ctggttaaag
     5521 gctgcgcaac acactgtttt gaggttgtag atagaactga cgaagtcagc tcaaagcact
     5581 gctttgaggt tgcagataga actgacgaag tcagctcaaa acatgttttt gaggttgtag
     5641 atagaactga cgaagtcagt aaccatacat acggtaaggc gacgtgacgt ggtttgcaga
     5701 gattttcgaa gagtattagc cttccatata gtctttcaaa gcctgtcctg ttagtccggc
     5761 attgagggcg atgaggagtt tcaagcgggc tttttgggcg ttgagttctt taacaaagaa
     5821 aacgcctgct ttttgcaact gtacgccccc accctggtag gcataaacag gctcggcaat
     5881 accgttaaag catcgtgata ccagagcgac tgggattcct ttttgcagaa ggctttctaa
     5941 tttttgagcc gtttctttgg gaatattacc agctccgaag gcttggataa tcaaaccgtc
     6001 caagtgttct aaatccagca tatcaatcag ctcatctgtc ataccagcat aagccgagat
     6061 gatagggact aaaccttgta tgtgatcaag gtcaaagcga acacgaggtt cagctgtttt
     6121 gaagtagagg atttcctgtt tcatgatgag accgaggggg ccatgtgttg gagtctggaa
     6181 ggtgttgaca ttagtcgtat gtgttttggt gacatacttg gcagcgtgga tttcatcgtt
     6241 cataacgacc aaaactcctt tgtcagcagc cctgtcatca ctggccactc gtaaagcact
     6301 taggtaatta taaacaccat cactaccgag ctcattggag ctacgcatgg ctcctgttag
     6361 aacgataggc atgtggggaa cttccatggt atcaaggaaa taggctgttt cctctaaagt
     6421 atcggttccg tgtgtgatca ccactccatc gtagttatct gcttcctctt taattttctg
     6481 gtagaggacc agcatatgtt tgggtttgat atggggactt ggaaggttaa aaaagtccaa
     6541 ggcgtggact tggattcctt caagtgggtt ggacacatgg ttcatgggat tatctgaact
     6601 cgtcacaaca gcgccagaag catcggcctg catggaaata gttccacccg tatgtaaaac
     6661 aaggattttc ttaggcatga tttactaact ctttctgaaa tgtggtataa tcattgtatc
     6721 acaaaagggg agattgggat aggatggaag tcaaagctgt tttttttgat atcgatggaa
     6781 ccttggtcaa caatcgcaag agtgttttga aatccactaa ggatgcgatt aagattgtca
     6841 aagaacaagg ggtactagtt ggcgtagcga cagggcgagg accttttttt gttaaggaat
     6901 tgatggacga tttggatctg gattttgcgg taacctataa tggccagtat atctttagta
     6961 aagacagagt cttgttcacg agccctattt ccaagttaca tttgcgccat ctaattagct
     7021 atgctaaaaa agagggcacg gagattgccc tagggaccaa ggatgccatg ttgggttcta
     7081 aaatcatgtc ctttggtctg ggttcttttt cccaacgaat tagtcgcttc gttccctctg
     7141 ttttaactcg gacggtgagt cagtctttta atcgcatggt cagcaaggtt gttccccaaa
     7201 aggaagagga cctgcttcac ctgatgaatc agcctatcta ccaagttttg atgctgatga
     7261 caccagaaga atctgagaag gcggcagctg attttgaaga cttgaaattg acacgtagca
     7321 atccttttgc atcggatgtc atcaatcagg gaaattctaa attagaaggc attcgccgag
     7381 ttgggaaaga atatggcttt gacctcaatc aagtcatggc ttttggtgac tcagataatg
     7441 accttgaaat gctggctggc gtcggtatgt cggtcgctat gggaaatggt agtagcagtg
     7501 tcaaggaagc tgccaagcac attaccacta gcaatcaaca agatgggatc cacaaggcct
     7561 tggaacattt tggtgttttg tcttcagaaa aagtctttgt cagccgtgac tatcatttca
     7621 ataaggtcaa gaccttccac cacatgatgg atgaacgaac ccaagaagaa cctcgagctt
     7681 gggatttaga gggtgcaacc catagggctg gttttaaaat agaagaattg gtggagtttg
     7741 ttcgagcagc cagtccttct gaagaagatt ttggtcaagc tgtatcgcaa cttcatcagg
     7801 cccttgataa ggcagcagat aaagtagcca agaagacacc tgctcagcaa gatttgatag
     7861 ggcaagtgga tgccttgatt gacacgcttt acttcaccta cggcagtttt gtcttgatgg
     7921 gggtggaccc agaacgtatt tttgatattg tccatcaggc caatatgggg aaaatttttc
     7981 cagatggcaa ggctcatttt gatccagtga cccataaaat cttaaaacca gataattggg
     8041 aagaaaaata tgctccagaa cctgctatta aaaaggaact gcagcgccag ctcaaggctt
     8101 acgaacgcca taaagagaga aataaatcgt aaacaaaaag gagctagggg ctccttttct
     8161 ttgtgattat aaggtttttt cttgttctct cacaaccagc aaatcgacct tagcatggcg
     8221 gagtatgtat tcagatgaag agccgaccaa gaggcgttca aaggcgttga gaccagttgc
     8281 gccaacgagg atgaggtcca cttcctcggc atctggaata gtacgtgcta gcagggtctt
     8341 tggatttccc atttcaatga cgatatgaac atctgctacc ccagcatctt tagcacgttt
     8401 ttcgtactct ttcatcagac tttcagcgtc gacttggagt tcttcgtaaa cttcagcatc
     8461 aaaggtggat acgctttgga gagcgcgtgt gtcaatgaca tgtgcgatgg tgagtttagc
     8521 gtcgtttcgt agagcagaat gaactccctt gacaaaagcc aagtccgctt ccttagaacc
     8581 atcgattgcg accatgatat tttggtaacg ttgtgccata atgaaacctc ctgaaatttt
     8641 cttactataa ttgtaaacct tttcactata gaagtaaagc aaaaaaagta tttttcaaaa
     8701 gaatattagg gcttaaattc tatataaata tatgataaaa taaaagaaag gatagcagga
     8761 aagaagtgaa ggaagggaga atgatgaaaa actcttttca aaaatcaaat ttcctttatt
     8821 atggaattat cctagtttca gtcttggtag aagtgatcat gatctgtcaa ttagagagtc
     8881 tggtaccgct tctttatcca agttttattg gatttttagt ttttcatgta ctctaccatc
     8941 taatcttatt tctagttgca aaacgaagtg ggcgttggga ttacttgatg atatgggggc
     9001 ttttcctcat gtttaatctt ctttatgact cctttctagg cttgcttttt ctaggtttat
     9061 cttttggtat gtgatgtgtc tttgcaaaat acctgctatc tctacgttct taacctcgcc
     9121 tttagcaagg tttttcttct atacgaaact agtaacacat ttcagtaaac ttgtcgcatt
     9181 ctctttctag tggtataatg ttactatcct gatgaattga ggaaacaaga tgaaagaata
     9241 taacaagtct agtaagttag agcatgttgc ttatgatatc cgtggtcctg tattggaaga
     9301 agccatgcgg atgcgagcga atggagaaaa gattttacgt ctgaatacag gaaatccagc
     9361 agaatttggc tttacagcgc cagacgaggt cattcatgac ttgattatga atgcgcgtga
     9421 tagtgaaggc tattctgact ccaaaggaat tttctcagcc cgtaaggcca tcatgcagta
     9481 ttgccagctg aagaaattcc caaacgtaga tattgatgat atttaccttg gaaatggtgt
     9541 cagtgagttg attgtcatgt ccatgcaggg gcttttggac aatggggatg aggtcttagt
     9601 gccaatgcca gactatcctc tatggacggc ggctgtcagc ctagctggag gaaatgccgt
     9661 tcactatatc tgtgatgaag ctgtggaatg gtacccagat attgacgata tcaagtcaaa
     9721 aatcacttcc aataccaagg caatcgtcct tatcaatcca aataacccaa ctggagccct
     9781 ttatcctaag gaactcttgc tggagattat tgagattgcc cgtcaaaacg atttgattat
     9841 ctttgcggat gaaatctatg accgcatggt aatggacgga catgtgcata cgcctgtggc
     9901 gagcttggca ccagatgtct tctgtgtcag catgaatggt ctgtcaaaat cccaccgcat
     9961 agcaggtttc cgtgtgggtt ggatggtctt gtctggccct aagactcatg ttaagggcta
    10021 tatcgaaggg ctcaatatgc tgtccaatat gcgcctttgc tctaacgttt tggcccaaca
    10081 agtcgtacaa acttccttgg ggggtcacca atcagtcgat gaattgcttc ttcctggtgg
    10141 acgaatctac gagcaaagaa atttcatcta taatgccatt caagatattc caggtttgtc
    10201 tgccgttaaa cccaaggcgg ggctctatat cttcccaaaa atcgaccgca atatgtaccg
    10261 tatcgatgat gatgagcagt ttgtccttga tttcttgaag caggaaaagg ttctcttggt
    10321 tcatggtcga ggctttaact ggcaggaacc agaccacttc cgtatcgttt accttcctcg
    10381 tgttgatgag ttagcccaaa tccaagaaaa gatgactcgt ttcttgaaac agtatcgtag
    10441 atagggcttg cattcgaaaa agctggaaac atttgcctag ggctattgac aaaagtgaaa
    10501 gaatcagata gaatagtaaa gtatgtttag taactgatca ctttatagcc ggctaaacga
    10561 atacaaaatc tatgaggagg tattcatcgt gaaacgtact tatcaaccaa gtaaacttcg
    10621 tcgtgcgcgc aaacacggat tccgtaaccg tatgtcaact aaaaatggtc gtcgcgtatt
    10681 ggcagctcgt cgtcgtaaag gacgcaaagt tttggctgca taatccaaac gaattctatc
    10741 aaaaatcagt aggaactcga gtctactgat ttttattttt gtaaaaaagt tcagtagatg
    10801 caaatggatt cggaagcgat gttatagtag attgaaacta gaatagtaca cctctgtttc
    10861 taaaacattg ttagaaatcg atttgactgt cctgatcgat ttgtcctgtt attattttat
    10921 tttactataa agttgaagta gctggagatg gcacagcgac aatcacattc ccagatggtt
    10981 cagtagtgac gattctagga aaagatacag ttcaacaatc tgcgaaaggt gaatctgtaa
    11041 ctcaagaagc tacaccagag tataagctag aaaatacacc aggtggagat aagggaggca
    11101 atactggaag ctcagatgct aatgcgaatg aaggcggtgg tagccaggcg ggtggatcag
    11161 ctcacacagg ttcacaaaac tcagctcaat cacaagcttc taagcaatta gctactgaaa
    11221 aagaatcagc taaaaatgcc attgaaaaag cagccaagaa caagcaggat gaaatcaaag
    11281 gcgcaccgct ttctgataaa gaaaaagcag aacttttagc aagagtggaa gcagaaaaac
    11341 aagcagctct caaagagatt gaaaatgcga aaactatgga agatgtgaag gaagcagaaa
    11401 cgattggagt gcaagccatt gccatggtta cagttcctaa gagaccagtg gctcctaatg
    11461 ctgctcctaa gacaacaagt gcaccgcaag caactgcagg aacaatgcaa gatgttacct
    11521 accagtcacc tgctggcaaa caattaccta acacaggttc agcatcaagt gcagcacttg
    11581 ctagtcttgg tctagtggtg gcaacaagtg gttttgcttt gctaggaaga aagactagac
    11641 gtagaaaata gaacagctag aaaattctat tctctactta aagttagatt ataaggggga
    11701 ttttgagaag tcatcaatcc tagtgatggg tgagaaaagt gagaacccaa gataatcaca
    11761 tactttagct gaataggaat attctatcaa tgtagccaat ctcttctgtc tctaactgtg
    11821 gaataggaga tgggcaatat cggatagaaa agatagcaga atagctctct attgaagaga
    11881 ggaggggaaa ccgaaaaatt aggtgcccct cctctttttt ggtataatag aaggatagaa
    11941 aacgaggtta gaagagatga tttttgatac acatacacac ttgaatgtag aagaatttgc
    12001 aggtcgtgag gcagaagaaa ttgccttggc tgctgagatg ggtgtgacac agatgaatat
    12061 tgttggtttt gataaaccga cgattgagca tgccttggag ttggtagatg agtatgagca
    12121 gctctatgcg actattggtt ggcatcctac agaagctggt acttatacag aggaagttga
    12181 ggcttacttg ttggataagt taaaacattc caaggttgtg gctttaggtg aaattggctt
    12241 agactaccat tggatgacag cgcccaaaga ggtgcaggag caggtttttc gccgtcagat
    12301 tcagctatct aaggacttgg atttgccttt tgttgtccat acccgtgatg cgctggaaga
    12361 tacctatgag attatcaaga gtgagggcgt tggtcctcgt ggtggtatca tgcattcatt
    12421 ttcagggacg cttgagtggg cagagaagtt tgtggatctt ggtatgacca tttccttctc
    12481 aggagtggtg accttcaaga aggcaactga cctccaagaa gcagctaaag agttaccttt
    12541 ggacaagatg ttggtagaaa cagatgcgcc ttacttagca cctgtaccca agcgtggtcg
    12601 tgaaaataaa acagcctata ctcgctatgt ggtcgacttt atcgctgact tgcgtggtat
    12661 gacgacagaa gagctggcgg tagcaacgac tgcaaatgca gaacgcattt ttggattgga
    12721 cagcaagtaa tgaaagagag aatttcccaa gttatcgtgg ttgaaggtcg tgatgatacg
    12781 gtcaatctca agcgttattt cgatgtggag acctatgaga ctcgaggttc tgccatcaat
    12841 gaccaggaca tagagcggat tcagcgtcta caccaacgtc atggagtcat tgtctttaca
    12901 gacccagatt ttaatgggga gcggattcgg cgcatgatta tgacggccat tccaacagtt
    12961 cagcatgcct ttctcaagcg agatgaagct gttcccaagt ccaagaccaa ggggcgttct
    13021 ctgggaattg agcatgccag ctatgaagac ctgaaaacgg ctctagctca ggtgacagaa
    13081 caatttgaac atgagagtca gtttgacatt agtcgtagcg atttgattcg ccttggtttt
    13141 ctagcagggg cagacagccg taagcgtaga gaatatctcg gagagactct ccgaatcggc
    13201 tattctaacg gcaagcaact cctcaaacgc ctagagttgt ttggggttac tttggcagaa
    13261 gtggaagaag ctatgaaatc ttatgagtag gaaagatgtg accgttacaa ttttttaagt
    13321 ttcacagtat ttttcgaagc aggtagaaga ggaggcgtct gatgttaatt ggtcaaaaaa
    13381 ttaaagagat tcggatagaa aaaggaatta gtcgtccaga tttttgtgga gatgagcaag
    13441 aactgacagt tcgtcaactg tcgcgaattg aaagtggagc ttcgcaaccg agtttgccca
    13501 agttagccta tattgctcgc cggctaggag ttccagttta tagccttatg ccggattttt
    13561 cagctcttcc ttctgcttat ttagaattga aataccagat tttacgtgaa ccaatctatg
    13621 gtaaagaaga ggagtacgat aagaaggaag cgtgtttgga agagatttat aaaacatact
    13681 ttgataatct tcctaaagaa gaacaattag catgtgaagt attgcaggcg tgtttggata
    13741 cttctagaac tagaaggcct gaatatgcag agttaatact tgaggaacat atgcctcaga
    13801 ttatagaaaa agaagcttat tcaataaatg atatgttgtt gattcgtttg tttttttatc
    13861 aaatgctcat tagaaaagat cttgccaaat ttataaatca aatcgaaaag ctaatgctct
    13921 ttcttttgga acagaagaag gtaactcaaa tagagaatta ctttataatt agagatactc
    13981 ttatttcagg aatgtgttgt cttgaaaagg taggagtaac tgattgtttt aatgattatc
    14041 tatcgtgttt acaagaaatt atggataaaa ctcaagatta tcaaaagaaa cctcttgtat
    14101 ttatgttttt gtggaagcaa gcattaagag tagaaagaga ttttagttta gctgaatcat
    14161 tttatcagtc ttctaaaaca tttgcgaagc taattggaga tggatttcta gtaaagaaat
    14221 tgacagagga atggcaagag gatgtcaaaa aatatttata aacatagtga atcagtgaca
    14281 aaaatgtcct tgtcctcgta tcaaaacagt tctaaagttc gtctttagaa ctgttttttt
    14341 agatataagc taaaaataat atgaaatagt tagattttaa ggacattgat gtccttgata
    14401 gtgtgataaa gatggagtat aatgactata aaagttcatt tcagggtgct attttgacaa
    14461 tttagagaaa aacaaataat tttagaatat catttggtaa tataatagta tcaacgttga
    14521 taatggaaag aggagcaagt aactcaacaa tcttgtaatc aaaaatagag gagtatcaat
    14581 atgaaacaaa cagttaaaaa attagctctt gtagcgagca ttgcagcaac attaggtgga
    14641 agtgtagcag ttgcctctgc agccgttcaa tacccagaag gtggtgtttg gacttatggt
    14701 tcaggtaacg gtggtgctta ctcaaactac tatcaccctt caaaatacca tagttcaaca
    14761 gttgttagca gaaaaactgg ttcatctgac aagggatatg ctggtgctgg agggacttct
    14821 cgtgcatgga ttcgtacttc ttggggagag aaagttgcat tctactataa tgtttagaat
    14881 ggtatagccc tattgtttag ctttacttgg tcaccttctc actaagtgac caagtaactt
    14941 tttggaggaa atgatgaaac gtttatttat tttgatttca atggtattgg tatcgcttta
    15001 tatggtgata acttccgttg accatcgaga agagatttta tttggtaact atccttctgt
    15061 tgatgtgaca ggaatgatga taaatcaacc agtagctagt cgggaagagg tgacagaggc
    15121 tttgagtcac ttggcggtag agcacaatag tctcattgct cgtcgaatcg ttgagccaaa
    15181 tgaagctgga gaaacacgct ttacctatgc cacttatggt gagggaaagc ttccagaagg
    15241 tctgaccatt tcctccaagg agagtgcaga aacgagtgat ttattagggt cttacttgat
    15301 tgtgtcagga agtttggatg gagtgagctt acagaccacc ttgaaagagc ttggttatca
    15361 aggctttgtt tcgaatggag aagatccatt ttcgatagtc ttactattga cggccacccc
    15421 tatggtgcta ctgagtttag ctatttttct gctgaccttt atgagtctga ccctgattta
    15481 tcggatcaaa tcccttcgtc aggcagggat tcgcttaata gctggtgaga gcttgtttgg
    15541 agttgctctc agaccagtgt tagaagatgt gagacagctt atctgctcag tgctggtatc
    15601 cagtcttttg ggattgggga ttctctggta tcaaggtgcc ttgtttatgg caacggtgca
    15661 actggtcatc attgctcttc tactttatgg attgaccttg gcagggattt ctaccttact
    15721 aagtgtcgtc tatctacttg gtttacagga aaatagtctg gtggatctat tgaaagggaa
    15781 actccctctc aaacgtatga tgacattgat gatggtgggg caactcttag ctgtattggt
    15841 ggtcggatcg agtgcgacag ctctcctacc ccactaccgt gaaatgcagg aaatggagag
    15901 agctagcaat aaatggagcc agtcctcaga ccgttaccgt ctatcctttg gttggtctag
    15961 tgcatttgcc gatgaagaag gaacgcgtaa ggataatcgt gagtggcaga catttactga
    16021 agaacggtta gccaatacag actcttttta tattatgagc aatgttgaca atttctcaga
    16081 tggagcagaa gtggacctag atggcaatcg tctcagtgac tacacaccgt cagggaatgt
    16141 tatctatgtc tcaccacgct atctgataga agaaaagatt accgtttctt cagagtttat
    16201 ggacaagatg caaaacttgt ctgagggaga gtttgggctg atcttgcctg agagcttgcg
    16261 agagcagtct gtctactacc aaggattgtt tacagattac ctgcaaaact tttcatctga
    16321 aagtgtagaa gtgacgagtc agaaacacta cctcccacag gtaaggctag cttttacaga
    16381 aacaggacag gaacgtttcc tctataatga tgggtacaag acaacacgcc agtacctaaa
    16441 agatccgatt attgtagttc taacgccgca agcgactgga gcaagacctg ttgcagggat
    16501 gttgtgggga actacggcta atagtgcctt gaaactagat cgatatggag acagcatcac
    16561 agctctaaaa gagcaaggtc tgtatcacaa ggtttcttac ttggtaaaaa gccagctatt
    16621 ttttgccaag gtactaaatg acaaacgggt ggagttttac tctctcctta ttgggacgat
    16681 tttgaccctg tctacggcta tcttgttatt tgattccatg aatcttctct attttgagca
    16741 gttcagacgg gatcttatga ttaaacgtct tgctggtatg acaatctatg agcttcatgg
    16801 caagtattta ctggcgcaag gaggagttct cttgcttggc ctagtcctat ctagtatttt
    16861 gacaagagat ggtttgatta gcgctctagt tgtagctttg tttacgctta acgccctctt
    16921 gattttagta aggcaggaca aaaaagaaga agctggtagc atggcagtat tgaaaggaaa
    16981 ataagatgat tgatattcaa ggattggaaa agaaatttaa tgaccgcgcg attttctctg
    17041 gtttgaatct caagctggag aagggcaagg tttatgcctt aatcggaaag agtggaagtg
    17101 gaaagacgac gctgctgaat atcttgggaa agctagaaaa gatagatggt ggaagggttc
    17161 tctatcaggg gaaagattta aaaaccattc ccactcgtga gtattttcga gaccagatgg
    17221 gctatctctt tcaaaatttc ggcctcttag aaaaccaatc aatcaaagaa aatttggatt
    17281 tgggttttgt tggtcagaaa atctcaaaag tagaacgttt ggaaaggcaa gtgggggctt
    17341 tagaaaaagt taatctaggg tatttggatt tagaacaaaa aatctatact ttatctgggg
    17401 gagaggccca acgagttgcc cttgctaaga ctattttgaa aaatccaccc ttgattttgg
    17461 cagatgaacc aacagcagct cttgatcctg aaaattcaga ggaggttatg aatctcttgg
    17521 tggatttgaa agatgaaaat cgaattatca tcattgcgac ccataatccc ctagtctgga
    17581 ataaggctga tgaaatcatt gatatgagga aacttgctca tgtgtgaaaa aatccgtatt
    17641 cgcagggtat ctgattatcc tagtgccaga ggtggtttag aagatatcct catcatggaa
    17701 aatatgacca atcatctcct tttggttcaa atccgagtgc atggctattt gcttgatttt
    17761 gctagtattg aagggcaaag gcaaaagcat tatcgtttga aaaatttacc tcagacggtt
    17821 gaactgacag tggatgatgt ggaggaggat gtggatttga ccctacccga aaatcgaagt
    17881 tatcaagaag ctgatttttt tgaacgcatg tttcgagaga actgctaagg ccacttttaa
    17941 agatttccaa gactatcttt cttcatgagg aaagatagtt ttttggtatg attttcattc
    18001 ccaaaataca aggggaatgt gttacaatag tagtaacaga taatagaaaa gagaatagat
    18061 gagaattgca gattatagcg tgaccaaggc agtgctggag cgtcacggtt ttacctttaa
    18121 aaagtccttc gggcaaaatt tcctgacgga taccaatatc cttcaaaaaa ttgtggatac
    18181 ggctgaaatt gatgatcagg tcaatgtcat cgaaatcggg ccaggtattg gtgccttgac
    18241 agaatttttg gctgagcgtg cagcccaagt catggctttt gagattgacc accgtttggt
    18301 gccaattttg gcagataccc tgcgtgattt tgataatgtg accgtagtta acgaagatat
    18361 tctcaaggtt gatttggcgc aacatatcca aaattttaaa aatcctaacc tgccaatcaa
    18421 ggtagtggct aatttgcctt actacatcac gacgcctatt ctcatgcact tgattgagag
    18481 tggcattcct tttagtgagt ttgtggtcat gatgcagaaa gaagtagcgg accgcatttc
    18541 agcccagcct aacaccaagg cttacggtag cttgtctatc gccgtgcagt attacatgac
    18601 agccaaggtt gcctttatcg tgcctcgtac ggtctttgtg ccagcgccaa atgtggattc
    18661 agccatcttg aaaatggtgc gtcgtccaga gccagccgta gcagtagaag atgagaactt
    18721 tttctttaag gtttccaagg ctagttttac ccatcgccgc aagaccttgt ggaataactt
    18781 gacaggttac tttggtaaga ctgaagaggt caaggacaag ctgaccaagg ctttggacca
    18841 ggcaggcttg tcaccaagtg tgcgtgggga agctctcagc ttggcagaat ttgccggtct
    18901 agcagacgca cttaaagggc aaggactcta agatgcaggg acaaatcatt aaagccttgg
    18961 caggtttcta ctatgtggag agtgatggcc aggtttatca aacacgcgcg cgtgggaatt
    19021 tccgtaaaaa aggccatacc ccttatgttg gggactgggt agatttctct gccgaggaaa
    19081 attcagaagg ctatatcctc aaaattcacg aacggaaaaa cagtctggtt cgtccgccta
    19141 ttgtcaatat cgatcaagct gtagtaatca tgtccgtcaa ggaacctgat tttaacagca
    19201 atttgctgga tcgtttcttg gttcttttgg agcacaaggg catccatccc attgtctata
    19261 tttccaaaat ggatttgttg gaagataggg gagaactgga tttttaccag cagacctatg
    19321 gtgacatcgg ctatgacttt gtgaccagta aagaggaact cctgtctttg ttaacaggca
    19381 aggttacggt ctttatgggg cagacaggtg ttgggaagtc aactcttctc aataaaatcg
    19441 caccagacct caatcttgaa acgggagaaa tttcagacag tctaggtcgc ggtcgccata
    19501 ccactcgagc tgttagtttt tacaatctca acgggggtaa aatcgcagat acaccaggat
    19561 tttcatcctt ggactatgaa gtatcaaggg ctgaagacct caatcaggct ttcccagaga
    19621 ttgctactgt tagccgagat tgtaagttcc gtacttgtac ccatacccat gagccgtctt
    19681 gtgccgtcaa accagctgtt gaagagggtg ttattgcaac cttccgtttt gacaattacc
    19741 tgcaattcct tagtgaaatt gaaaatcgta gagaaaccta taaaaaagtc agcaaaaaaa
    19801 ttccaaaata aggagaaacc tatgtctcaa tacaagattg ctccgtcaat tctggcagca
    19861 gattatgcca actttgaacg tgaaatcaaa cgtctagaag caactggggc agaatatgcc
    19921 catatcgata tcatggacag tcattttgta ccgcaaatca gttttggtgc aggtgtggtc
    19981 gagagccttc gtcctcatag taagatggtt ttcgattgcc acttgatggt gtcaaaccct
    20041 gagcatcatc tggaagattt tgcgcgtgca ggtgcagaca tcatcagtat ccatgtagaa
    20101 gcaacacctc atattcatgg cgccctccaa aaaattcgtt cactcggagt taagccttca
    20161 gtcgttatca atcctggcac accagttgaa gccatcaagc acgtccttca tctagttgac
    20221 caagttttag tcatgacggt taatccaggt tttggtgggc aagcctttct gccagaaacc
    20281 atggataagg ttcgtgagtt ggttgctctt cgtgaggaaa aaggtttgaa ctttgaaatc
    20341 gaagtggatg gtgggattga tgaccaaact attgctcaag ccaaagaagc tggtgcgact
    20401 gtttttgtag caggttccta tgtctttaag ggagaagtca atgagcgagt acaaactctc
    20461 agaaaacaac tggactaggg ttgcagtttt tgcaggcgga aaccgcggtc attatcggac
    20521 agattttgat gcttttgttg gggtggatcg aggctcgctc tgggtcttgg aagaagactt
    20581 acctcttgct ctagcagtcg gagattttga ttctgtgacg gaagaagagc gacaggtgat
    20641 tcaaaaacgt gcccagtatt ttgtccaagc acgaccagaa aaggatgata cagatctgga
    20701 attggctctc ttaaccatct ttgaacaaaa tcctcaggct caggtcacta ttttcggtgc
    20761 cttgggtggc cgtattgacc atatgttggc caatgtcttt ctacctagca atcctaagtt
    20821 ggcaccctat atgcgtcaaa tagaaattga ggatgggcaa aatttgattg cctattgttc
    20881 agaagggacc agtcagctag aacctcgttc agactacgac tatctagcct ttatgccagt
    20941 tcgggatagc cagctgacta ttcttggagc taagtatgag ttgacagagg agaatttttt
    21001 ctttaaaaaa gtgtacgctt ctaacgaata tatagatagg gaagtgtcgg taacttgccc
    21061 agatggttat gtggtcgtac tgcatagcaa ggacaggagg taggatggaa agtttactta
    21121 ttctattatt aattgccaac ctagctggtc tctttctgat ttggcaaagg caggataggc
    21181 aggagaaaca cttaagtaag agcttggagg atcaggcaga tcatttgtca gaccagttgg
    21241 attaccgctt tgaccaagcc agacaagcca gccagttaga ccaaaaagat ttggaagtgg
    21301 ctgtcagcga ccgtttgcaa gaagtgcggc ttgaattgca ccaaggcctg acccaagtcc
    21361 gtcaagaaat gacagataat ctcctccaaa ctagagacaa gacagaccaa cgtctccaag
    21421 ccttgcagga atcaaatgag caacgtttgg aacaaatgcg ccagacggtc gaggaaaaac
    21481 tagaaaagac cttgcagaca cgcttacagg cttcctttga gacagtttct aaacaactgg
    21541 agtctgtcaa tcgtggtctt ggagaaatgc agacagttgc ccgtgatgtc ggagctctta
    21601 acaaggttct ctctggaacc aagacgcgag ggattctggg agaattgcaa ctggggcaaa
    21661 ttattgaaga catcatgaca cctgcccagt acgaacgaga atacgcaacg gttgaaaact
    21721 ctagtgaacg agtggagtat gccatcaagt tacccggaca aggcgaccaa gaatacgtct
    21781 acctaccaat tgactctaag tttccactgg cagattatta ccgcttagaa gaagcctatg
    21841 aagtaggtga caaggatgaa atcgaacgct gtcgtaagtc gctcctagca agtgtcaagc
    21901 gttttgctag ggatattagg aacaagtaca tagcaccacc tcggacaacc aattttggaa
    21961 tcttgtttgt tccgacagaa ggtctttatt cagagattgt gcgcaatccg gtcttctttg
    22021 atgatttgag acgggaagaa cagattatcg ttgcaggacc aagtacccta tcagcccttc
    22081 ttaactccct atcagttggt ttcaagaccc ttaatatcca aaagagtgcc gaccatatca
    22141 gcaagactct tgccagtgtc aagaccgagt ttggcaagtt tggtggtatt ctggtcaagg
    22201 cacaaaaaca tctccaacat gcctctggca atattgatga attattaaac cgtcgtacca
    22261 tagctatcga gcggacgctc cgtcacattg agttgtcaga aggtgagcct gcgcttgatc
    22321 tactccattt tcaagaaaat gaggaagaat atgaagatta gtcacatgaa aaaagatgag
    22381 ctatttgaag gcttttacct aatcaaatca gctgacctga ggcaaactcg agctgggaaa
    22441 aactacctag cctttacctt ccaagatgat agtggcgaga ttgatgggaa gctctgggat
    22501 gcccaacctc ataacattga ggcctttacc gcaggtaagg ttgtccacat gaaaggacgc
    22561 cgagaagttt ataacaatac ccctcaagtc aatcaaatta ctctccgcct gcctcaagct
    22621 ggtgaaccca atgacccagc cgatttcaag gtcaagtcac cagttgatgt caaggaaatt
    22681 cgtgactaca tgtcgcaaat gattttcaaa attgaaaatc ctgtctggca acggattgtc
    22741 cgaaatctct acactaagta tgataaggaa ttctactcct atccagctgc caagaccaac
    22801 caccatgcct ttgaaacggg gttggcttat catacggcga ccatggtacg tttggcagac
    22861 gctattagcg aagtttatcc tcagctcaat aagagtctgc tctatgcggg gattatgttg
    22921 cacgacttgg ctaaggtcat cgagttgacg ggaccagacc agaccgagta cacggtcaga
    22981 ggcaatcttc ttggacatat cgctctgatt gatagcgaaa ttaccaagac agttatggaa
    23041 ctcggcatcg atgataccaa ggaagaagtc gttttgcttc gtcatgtcat cctcagtcac
    23101 cacggcttgc ttgaatatgg aagtccagtc cgtccacgta ttatggaggc agagattatc
    23161 catatgattg acaatctgga tgcaagcatg atgatgatgt caacagctct ggctttggtg
    23221 gataaaggag agatgtccaa taaaatcttc gctatggata atcgttcctt ctataaacca
    23281 gatttagatt aataatttaa gaaaaatgag cattttttag gataagaatg ttcgtttttt
    23341 tatgtgaata tggtataata agtaaaagac aaaaatgaat actcttcgaa aatctcttca
    23401 aactagggta gtatcgcctt gtcgtatgta tatatgcagg tatattacag ggtttgtcag
    23461 ttctattgac aatctcaaaa cagtgttttg aaccaccagc gaccagcttt ctagtttgct
    23521 ttttgatttt ttgaataaaa atggaatagg aaatagaaat gaaattaaga agaagtgatc
    23581 ggatggttgt catttccaac tatttgatta ataatcctta taaactaact agtctcaata
    23641 cttttgctga aaagtatgag tctgctaaat catccatctc agaagatatc gtcattatca
    23701 aacgcgcctt tgaggaaatt gaaatcggtc atatccagac agtgactggg gctggcggag
    23761 gtgtcatctt cacaccgtct atttcgagtc aggatgctaa ggaaatggtt gaagacttgc
    23821 gtaccaagtt gtcagaaagt gaccgtatct tgccaggtgg ttatatctat ctgtctgatt
    23881 tgcttagcac accagccatc ttgaaaaata ttggtcgtat tattgccaaa agctttatgg
    23941 accaaaaaat tgacgcggtt atgaccgtag caactaaggg tgtgccactt gcaaatgcag
    24001 ttgccaatgt cctcaatgtc tcttttgtca ttgtgcgccg tgacctgaaa attaccgaag
    24061 gttcaactgt tagcgtcaac tatgtttcag gttcaagtgg tgaccgtatc gagaaaatgt
    24121 tcctttcaaa acgtagtctt aaggcaggca gccgtgtctt gattgtggat gacttcttga
    24181 aaggtggcgg aacagtcaat ggtatgatta gtctcttgcg cgagttcgac tcagaactgg
    24241 caggtgtagc ggtctttgcg gacaatgccc aagaagaacg tgaaaagcag tttgactaca
    24301 agtcactctt gaaggtaacc aatattgatg tcaagaacca agccatcgat gttgaggttg
    24361 gcaatatctt tgacgaagat aaataagaga tagaactaaa ggttggaacg attgtcccag
    24421 cctttctttg caaacagaat agaaggaagc ttatgaaaac accatttatc aatagagaag
    24481 agttagaagc gattgttgcc gagttcccga ctccctttca cttgtatgat gagaagggga
    24541 ttcgtgagaa ggcaagagcc gttaaccaag ctttttcgtg gaacaagggc tttaaggaat
    24601 attttgcagt taaggctact ccaactccag ctattttgaa aattctccaa gaagaaggtt
    24661 gtggtgtgga ctgctctagt tatgtagagc ttttgatgag ccataaactg gactttctgg
    24721 gttctgagat tatgttctct tccaacaaca cgccagacaa ggaatacgcc tatgcacgtg
    24781 aattgggtgc gaccattaac ttggatgcct ttgaagatat tgaacatctg gagagagtag
    24841 caggcattcc agaaatcatc tcttgtcgtt ataatcctgg aggcgttttt gaactgggga
    24901 cagacattat ggacaatcct ggggaggcta agtttggcat gaccaaggac cagctctttg
    24961 aagcctttgc tatcttgaag gaaaaaggag ccaagacttt tgggattcac tccttcctag
    25021 cgtccaatac cgtgacccat ctctattatc cagagttggc tcgtcagctc tttgaactgg
    25081 ctgttgaaat caaggaaaag ttgggcattt cgctagactt tatcaatctt tctggcggta
    25141 ttggtgttaa ttatcgtcca gaccaggagc cgaacgatat cgccttgatt ggtgagggag
    25201 ttcgtaaggt gtatgaagaa gttcttacgt cagcaggtct tggtcaggtc aagattttca
    25261 ccgaattggg tcgttttatg ctggcacctc acggtgctct agtcacaaga gtgactcata
    25321 agaaggaaac ctaccgtacc tatctaggtg tggatgcctc agcagtcaac ctcatgcgtc
    25381 cagctatgta cggagcttac catcatatta gcaacgtgac ccatccagat ggaccagctg
    25441 aagcggtaga tgtggttggt tcactctgtg aaaacaatga taaatttgca gtcaatcgcg
    25501 aactgcctca tacagaaatc ggtgatttgc tggtaattca tgatacaggt gcccacggat
    25561 tttcaatggg ctaccagtat aatgccaaat tacgttctgc ggaaatcctc tataccgaag
    25621 aaggtaaagc ccgtcaaatc cgccgtgcag agcgccctga ggactatttt gcaaccttat
    25681 atggcttcga ttttgaagaa taatctgata atagattgaa aatgaaattg aaaaacagat
    25741 tgctttctaa aaaataggca aaaatcttgt ttttccttca agtcgtgata taataaaact
    25801 ataaaacgtt ttcaaggaag gtaacgatat gtctgaagaa acaattgatt atggacaagt
    25861 gacaggaatg gtgcattcga cagaaagctt tgggtcagta gatgggcctg gtattcgctt
    25921 tattgtcttt ttgcagggct gtcacatgcg ttgccagtat tgccacaacc cagacacttg
    25981 ggctatggag tccaataagt cacgtgaacg gacggtagat gatgtcttga cagaggcctt
    26041 gcgctaccgt ggtttctggg gaaataaggg tgggattaca gtcagtggag gagaagctct
    26101 cttgcagatt gatttcctga ttgctctctt caccaaggct aaggaacaag gaatccactg
    26161 taccttggat acctgtgctc ttcctttccg taataaacca cgttaccttg agaagtttga
    26221 caaactcatg gctgtcactg acttggttct tttggatatc aaggaaatca acgaagaaca
    26281 gcacaagatt gtcactagcc aaactaataa aaatatcttg gcttgtgccc agtatctatc
    26341 agatattgga aaacctgtct ggattcgcca cgtgctagtt ccaggattga cagacagaga
    26401 tgatgacttg attgaacttg gtaagttcgt caagaccctc aaaaatgttg ataagtttga
    26461 aattctacct tatcacacca tgggtgagtt caagtggcgt gaacttggaa ttccatattc
    26521 cctcgaagga gtcaaaccac caacagcaga tcgcgtcaag aacgctaaaa aactcatgga
    26581 taccgaaagt tatcaagatt atatgaaacg tgtacatgga tagaaaagaa acctgatgga
    26641 aacatcgggc ttttgatttg caaaaagact tagcaaatca gctaagcctt tttcttctta
    26701 tctcgaacgt tgttttccag cgttgcgatt tttgtgtttt ttcttgcttg tgatagcagt
    26761 tggttgttca ggggtaacgt cttttcgtcc acttggttta gagaaagcac ttgcttttgg
    26821 tgggttcttg gctagttctt cacggacttt tttgcgaagt tttggacgaa cgatatagtt
    26881 gacgataaac tgttggagaa tcatcatgaa accaccgaca acccagtaaa gtgtgacact
    26941 agctggtgag aagagggaga agacgacgat catgagtggg ctcatgtaaa tcattttctt
    27001 gatttgttct ctttgcattt catcttctac tccgtgaagt gaaaggagcg attgaagata
    27061 gtaaaggaca ccagcacagg caaccaaaat catacttgga gaacctagag gaatgcctag
    27121 gtagcttgct tgagcaaccc cttcagtatg ttgggcagca aagtagatag cagagaagaa
    27181 aggcatttga aggaggatag ggaaacatcc tacaccacca aacatgctga tgccgtgctc
    27241 tttttgagcg gcaaagaggg cttgttgggc ttcgagtttt tcttcttgag tagttgcttc
    27301 tttgagacgt gtttggtgtg gctcaaggac atgcttgaga gcgttcatct tttcagagtg
    27361 aagcgttgcc ttccatgatt ggtagatacc aagtggtaag ataatcaagc gtacgataat
    27421 ggttacgata atgatagcga caccaaagcc tagaccttta tcagtagcga agtacttgat
    27481 ggcttcagcc ataggcgctc cgatcgtatt ccaaataaat cctgttggct gacctgtggt
    27541 tttatcgaca ttgacacagc cagtcaagac aagcaacata gccactccca tagccgagaa
    27601 tgcaaaacgt ttaatagatt tcactgtttt tattccttct ttaaaaatta tacctttcta
    27661 ttctactgtt tttttacaaa atatacaata gttctggaga cctaatttgc gattttaaag
    27721 tcagagtagg gaggaaagtt gcttagtttg acatctaagt agcttacttt tgaaaaaggt
    27781 gtcggtcctt ttcggatttc ttggataaat tttgccatga tagcagatga gtctgcttgg
    27841 gctaagattt ccactgtgcc atcgtcgtta ttccataccc gacctgtgat gccaccgatt
    27901 tcaagggcca ggctataaac accccaacga aagccgactc cctgcaccct accttgggca
    27961 atcattctaa ccttttgcat accaaacccc tttgattgtg ttataatatt tctatgacta
    28021 ttataacctc aaaagccaat tctgtggtaa aaaatgccaa gaaattacac caaaaaaaat
    28081 accgcaagtc tgcctatttg attgaaggct ggcacttgtt tgaagaagct gtccaagctg
    28141 gagtgacgat tgagaaaatc tttgccctag aaaattatcg aaatcagtta gctgcttttc
    28201 cgcaaactgt ctggatttca gaggatattt tgctagattt ggcggattct cagactccac
    28261 agggaattgt tgccgtggtt caaaaagaag aagtaggaca agctgatttg agtcagggca
    28321 agttcttgtt tttggaagat gtgcaagatc ctggtaatgt aggaactatc attcgaactg
    28381 cggatgcagc aggttttaca ggagtgattg tttcagataa gtcggcagac atctacagtc
    28441 tcaaaaccct acgttccatg caaggtagtc atttccatct gcccatttac cggatgacta
    28501 gtcaagcgct tcttgacgaa actaaaaagg tagctatccc agtgctagca acaaccctat
    28561 ctaaagattc tgttgattac agagaactgc cttctataga aaattttgta ctagttatgg
    28621 gaaatgaggg tcaaggaatt agtcccctta tggctgaaag tgcagaccag ttggtccata
    28681 ttagcatgaa ggggcaggcc gagagtttga atgttgcagt tgcaaccggt attttaatct
    28741 tccatttaag ctaattttaa ctttttttgt tataatcaag gaaagatgtt cacagaaaag
    28801 gagaaaagga tgaatcacac tattatacat gaccgtgcag gtctcaatca attttacgct
    28861 aaggtttatg cctttgttgg tctgggaatc ggactatctg ctttggtatc aggccttatg
    28921 ttgacggtct ttcagtctca gttggtttac ttgttgatgc aggggcgtct ctggttgacc
    28981 attgctactt ttgcggagtt ggctttggtt ttcgttgcca gtagcatggc ttcaagaaat
    29041 agtccagctg ctcttccagt atttttactt tactccgttt taaacggctt tacccttagt
    29101 tttgtggtgg ctttctatac tccgggtaca gttttatctg cctttgtatc gagcgccctt
    29161 ctcttctttg tcatggcggc agttggtatt tttaccaaga aagacttgag tgggattggt
    29221 cgagcaatga tggcagctct cattggttta ctgattgcta tggtagtcaa tattttcttg
    29281 gctagtggct tctttgatta tatgattagc gtagccatgg ttttggtttt ttcagggttg
    29341 attgcttggg ataaccaaaa gattcgcctc gcctatgaac aatcacaagg tcgtgtagcg
    29401 acaggctggg tggtgtcaat ggctctcagc atctatcttg attttatcaa tcttttccta
    29461 agtattctac gtatctttgg tcgcaatgac taaaaggtct atgaaatcag tgttttgagt
    29521 gacactgatt tttttaaatt ttctcttttc ttttcttgga aataggtgta taatacgttt
    29581 attaaatttt tgaggagttg tctatgaaga aaagttttat ccatcaacaa gaagaaattt
    29641 cctttgtcaa aaatactttt acccagtatt tgaaagataa gctagaagtt gtcgaagttc
    29701 aaggtcctat cttgagtaag gtcggtgacg gaatgcagga caacctgtct ggtgtggaaa
    29761 atccagtatc ggtcaaggtt ctccaaatcc ctgatgctac ttatgaagtg gtgcactcac
    29821 ttgctaaatg gaaacgccac accttggctc gttttggctt tggtgaagga gagggtctct
    29881 ttgtccacat gaaagccctt cgtccagatg aggattcctt ggatgcaacc cactctgttt
    29941 atgttgacca gtgggactgg gagaaggtta tcccaaatgg taaacgtaac atcgtttatc
    30001 taaaagaaac agttgagaag atttataagg ctattcgcct gactgagcta gctgttgaag
    30061 cccgctatga catcgagtct atcttgccaa aacaaatcac ctttatccat acagaagaat
    30121 tggtagaacg ctacccagac ttgacaccta aagaacgtga aaatgcgatt tgtaaagaat
    30181 ttggagccgt ctttttgatt ggtatcggtg gcgagttgcc agatggtaaa ccgcacgatg
    30241 gacgtgcacc agactatgat gactggacaa gcgagtctga gaatggctac aagggtctaa
    30301 atggtgatat tcttgtctgg aatgagtctt taggtggagc ctttgagttg tcttctatgg
    30361 gaattcgtgt agatgaagaa actcttagac gtcaggtgga aattacaggt gatgaagacc
    30421 gcttggaatt ggaatggcac aagtctttgt tgaatggcct attcccattg acaatcggtg
    30481 gaggaattgg acaatctcgt atggccatgt tcctacttcg caagagacac atcggagaag
    30541 tgcaaacaag tgtttggcct caagaagtcc gcgatactta cgaaaatatt ttgtagagaa
    30601 tcgaaccgca aggttcggtt ttctttctct ttttgtctat aatttggtat aataaacagt
    30661 atgaaaatcg tatcaggaat ctatggggga cgtcccctca agacactaga aggcaagaca
    30721 acaagaccta cttcggataa ggttagggga gccattttta acatgattgg tccctacttt
    30781 gaagggggac gagtcttgga cctttatgca ggtagtggtg gtttatctat cgaagcagta
    30841 tcgcgtggca tgtccagtgc tgttttggtg gagcgagacc gtaaggctca gaccatcgtg
    30901 gctgaaaata tccagatgac caaggaagtt ggaaaatttc aactcctcaa gatggatgca
    30961 gagagggcat tggaacaggt atctggggaa tttgacctcg ttttcttaga ccctccctat
    31021 gccaaggaac aaatcgtagc agatattgaa aaaatggcta agagagagct tttttctgaa
    31081 gatgttatgg ttgtgtgcga gacggataag gccgttgaac ttccagaaga gattgcctgt
    31141 ctgggtatct ggaaggaaaa gatttatgga attagtaagg tgacagtcta tgtcagataa
    31201 gattggctta ttcacaggct catttgatcc gatgacaaat gggcatctgg atatcattga
    31261 acgggcgagc agactttttg ataagcttta tgtgggtatt ttttttaatc cccacaaaca
    31321 aggatttctc cctcttgaaa atcgtaaacg ggggctagaa aaggctttgg gacatttgga
    31381 aaatgttgaa gtcgtggctt ctcatgatga attggtggtc gatgttgcaa aaagactggg
    31441 ggctactttc ctagtacggg ggttgaggaa tgcggcggat ttgcaatatg aagctagttt
    31501 tgattactac aatcatcagc tgtcttctga tatagagact atttatttac atagccggcc
    31561 tgaacatctc tatatcagtt catcaggtgt tcgagagctt ttgaagtttg gtcaggatat
    31621 tgcctgccat gttcccgaga gtattttgga ggaaataaga aatgaaaaaa aagattagat
    31681 ggcccttata cgtcattgcg gccttgattg tgactttctt ggcatttgta gtgcccttgc
    31741 cttattatat agaggttcca ggtggttcgg aagatattcg ccaagtcctt aaagtaaatg
    31801 acacagaaga taaggaagct ggtgcctatc aattcgttac ggttggtgtt caacatgcca
    31861 ctttagctca tatgatttat gcttggttga caccttttac agatattcgt agtgctcagg
    31921 agactacagg tggttcttcc gatgttgaat ttatgcgaat caatcaattc tacatgcaaa
    31981 catcgcaaaa tatggccaag tatcaaggac taaaaacagc tggtaaggat atcgaactca
    32041 agtactttgg agtttatgtt ttgaatgtga cggataattc aacctttaaa gagattctca
    32101 atatctctga tacagtcaca gcagtcaatg atcagacctt tgatagttcc aaagacttga
    32161 ttgattacgt cagttctcaa aaattagggg attccgtcaa ggtcacctat gaagaggatg
    32221 ggcaaaccaa gtctgcagaa ggaaaaatca tcaccttgga aaatggcaaa aatggaattg
    32281 gaatcggctt gattgaccgt acagaggtaa tcagcaatgt cccaattagc ttttcaacag
    32341 ctggtattgg cggtccaagt gctggtctca tgtttagtct agctatctat actcaaatag
    32401 ctcacccaga tcttcgtaat ggtcgtattg ttgccggtac aggtaccatt gaccgcgatg
    32461 gtaatgtggg agacattgga ggtattgata agaaggttgt agcttcggct agggcaggtg
    32521 ctgctatttt ctttgctcct gataaccctg ttagcgaaga agaacaaaag gcgcatccgg
    32581 acgcgaaaaa caactaccaa acagccctag aagcggctaa aacaatcaag acggatatga
    32641 aaatcgtgcc cgttaaaacc ctacaagatg cgattgatta cttgaaaaac aatccctaat
    32701 ttttgagtga agtttccaaa ctagcgcaca aacagagaag atggtataat agtcaaatgg
    32761 ttcaattatt attcactcta agtagtcata tgttctttat ttatgtgagt ttttaccttt
    32821 taaaggatct tgttagatgg gagaaggttt taaaagtgac agatgataat acaagaaaag
    32881 ttcgtttatt agtagccttt tttagcattg tcataggcta catcctgagt tctttcttta
    32941 ttagcctgta tcatttgtgg caagaagcgc ttagaggatt attatgaaat caagagtaaa
    33001 ggaaacgagt atggataaaa ttgtggttca aggtggcgat aatcgtctgg taggaagcgt
    33061 gacgatcgag ggagcaaaaa atgcagtctt acccttgttg gcagcgacta ttctagcaag
    33121 tgaaggaaag accgtcttgc agaatgttcc gattttgtcg gatgtcttta ttatgaatca
    33181 ggtagttggt ggtttgaatg ccaaggttga ctttgatgag gaagctcatc ttgtcaaggt
    33241 ggatgctact ggcgacatca ctgaggaagc cccttacaag tatgtcagca agatgcgcgc
    33301 ctccatcgtt gtattagggc caatccttgc ccgtgtgggt catgccaagg tatccatgcc
    33361 aggtggttgt acgattggta gccgtcctat tgatcttcat ttgaaaggtc tggaagctat
    33421 gggggttaag attagtcaga cagctggtta catcgaagcc aaggcagaac gcttgcatgg
    33481 cgctcatatc tatatggact ttccaagtgt tggtgcaacg cagaacttga tgatggcagc
    33541 gactctggct gatggggtga cagtgattga gaatgctgcg cgtgagcctg agattgttga
    33601 cttagccatt ctccttaatg aaatgggagc caaggtcaaa ggtgctggta cagagactat
    33661 aaccattact ggtgttgaga aacttcatgg tacgactcac aatgtagtcc aagaccgtat
    33721 cgaagcagga acctttatgg tagctgctgc catgactggt ggtgatgtct tgattcgaga
    33781 cgctgtctgg gagcacaacc gtcccttgat tgccaagtta cttgaaatgg gtgttgaagt
    33841 aattgaagaa gacgaaggaa ttcgtgttcg ttctcaacta gaaaatctaa aagctgttca
    33901 tgtgaaaacc ttgccccacc caggatttcc aacagatatg caggctcaat ttacagcctt
    33961 gatgacagtt gcaaaaggcg aatcaaccat ggtggagaca gttttcgaaa atcgtttcca
    34021 acacctagaa gagatgcgcc gcatgggctt gcattctgag attatccgtg atacagctcg
    34081 tattgttggt ggacagcctt tgcagggagc agaagttctt tcaactgacc ttcgtgccag
    34141 tgcagccttg attttgacag gtttggtagc acagggagaa actgtggtcg gtaaattggt
    34201 tcacttggat agaggttact acggtttcca tgagaagttg gcgcagctag gtgctaagat
    34261 tcagcggatt gaggcaagtg atgaagatga ataagaaatc aagctacgta gtcaagcgtt
    34321 tacttttagt catcatagta ctgattttag gtactctggc tctaggaatc ggtttaatgg
    34381 taggttatgg aatcttgggc aagggtcaag atccatgggc tatcctgtct ccagcaaaat
    34441 ggcaggaatt gattcataaa tttacaggaa attaggctgg agaaccagcc tttttctaaa
    34501 gataaggaga aatatgaaca aaaaaacaag acagacacta atcggactgc tagtgttatt
    34561 gcttttgtct acagggagct attatatcaa gcagatgccg tcggcaccta atagtcccaa
    34621 aaccaatctt agtcagaaaa aacaagcgtc tgaagctcct agtcaagcat tggcagagag
    34681 tgtcttaaca gacgcagtca agagtcaaat aaaggggagt ctggagtgga atggctcagg
    34741 tgcttttatc gtcaatggta ataaaacaaa tctagatgcc aaggtttcaa gtaagcccta
    34801 cgctgacaat aaaacaaaga cagtgggcaa ggaaactgtt ccaaccgtag ctaatgccct
    34861 cttgtctaag gccactcgtc agtacaagaa tcgtaaagaa actgggaatg gttcaacttc
    34921 ttggactcct ccaggttggc atcaggtcaa gaatctaaag ggctcttata cccatgcagt
    34981 cgatagaggt catttgttag gctatgcctt aatcggtggt ttggatggtt ttgatgcctc
    35041 aacaagcaat cctaaaaaca ttgctgttca gacagcctgg gcaaatcagg cacaagccga
    35101 gtattcgact ggtcaaaact actatgaaag caaggtgcgt aaagccttgg accaaaacaa
    35161 gcgtgtccgt taccgtgtaa ccctttacta cgcttcaaac gaggatttag ttccctcagc
    35221 ttcacagatt gaagccaagt cttcggatgg agaattggaa ttcaatgttc tagttcccaa
    35281 tgttcaaaag ggacttcaac tggattaccg aactggagaa gtaactgtaa ctcagtaaaa
    35341 gataagccta aactcctatg tcacttatgg atgtaggagt tctttttact agtttaagca
    35401 gggctagaac aggtactaag aaaaaatagc aacttctaaa actaacttcc agttttggga
    35461 gagagatgga agttactttg agaagttacg gtaagaaaaa ataatatgta ttggattaaa
    35521 ttttgtgata taataaagga taagatacta ttttaggaga agaactatgg aagacccaag
    35581 tagtcagaat ttgttgctac aatttgtttt attgtttatc ttgacggtat taaatgcctt
    35641 tttctcagcc acagaaatgg ccatggtttc tcttaatcgt gcccgagttg aacaaaaggc
    35701 ggaagaagga gacagacgct acatccgcct gctgaaggta ctagaaaatc ctaaccactt
    35761 tttatcaacc attcaggtag gaatcaccct gattacaatc ttatcagggg cgagtttaac
    35821 taatactcta ggacgtgtga ttgcctcttg gcttgggaat ggcgaaacag cacaagccgt
    35881 ggcaactttt ctatccttgg catttttgac ctatatttcc atcgtttttg gggaattata
    35941 tcctaagaga atcgctctta atctaaagga tgccttggca attcgtacag cgccggttat
    36001 cattgggatt ggaaaactag tcagtccttt tgtttggctt ttagctgctt cgaccaattt
    36061 cttgagccat ttgactccta tgtcattcga tgatgcggat gaaaagatga cccgtgatga
    36121 aattgcctat atgctgacaa atagtgaaga aacattggat gctgatgaga ttgagatgct
    36181 acaaggtgtc ttttcgctcg atgaactgat ggcacgagag gttatggttc ctcgaacgga
    36241 tgcctttatg gtggatattc aggatgatag tcaagccatt atccaaagta ttttaaaaca
    36301 aaattattct cgtatcccgg tttatgatgg ggataaggac aatgtaattg gaatcattca
    36361 caccaagagt ctccttaagg caggctttgt ggacggtttt gacaatattg tttggaagag
    36421 aattttgcaa gatccacttt ttgtacctga aactattttt gtggatgact tgctaaaaga
    36481 actgcgaaat acccaaagac aaatggcaat cttgctggat gaatacggtg gtatggctgg
    36541 tttggtgact ttggaagacc tcttagagga aattgttggt gaaattgatg atgaaacgga
    36601 caaagcagca atagatgttc atcagattgg cgaggatacc tacattgttc aaggaactat
    36661 gaccctcaat gatttcaata attactttga tgttgaactg gaaagtgatg acgttgatac
    36721 catcgctggt tattatttga caggagtggg aactattcca acgactgaga aactcagcta
    36781 tgaattggtt agccaaaaca aacagtttat cttaaccaat gataaagtga agaatggacg
    36841 tgttaccaag gtaaaagtcc aaatcacaga agtcgaaata gaagaagaaa cagaataaac
    36901 tagaggcttt tgtcaccagg cgtgacgaag cttttttcaa ttgatttcaa aagggaatta
    36961 tgccatggaa agaactctgc cacaaactcg ctccaaaagt ttttaaagtt atacgtattt
    37021 attcgaggga aaataaaaaa agcccttcaa attgggcttt ctgttcattt gaaacctgat
    37081 aaatcaagtt atagaaggcg gtagacggat ttgaaccgac gatcaagctt ttgcagagcc
    37141 gtgccttacc acttggctat accgccttaa cgtttattat tataccttga aaatgatttt
    37201 cagtcaatag ttttttcagt tttttgtacc atagcgtgat tcgaatgtga tgccaagttt
    37261 tctatgggaa aaaatttacc aaaaacctac tgaaacccct tgaaaaatct agctaaatag
    37321 ggtataatat gggtaatcat ttgtcgtagg ttttgtctga aatattgtcc agacaaggct
    37381 cacagcagtt aaatcttctg aaaaagtcag atttagctgc tctttttgtg ctttttttca
    37441 agattttgag catttgtaac agaggcttaa agattctgaa aattcatcaa gaggacacgg
    37501 tgataagggg tttacaacca tatgacgatt agaaaagcct gattgacaag gcttggaact
    37561 tatttacaaa ggagaatcat cttggcagga catgacgttc aatacgggaa acatcgtacc
    37621 cgtcgtagtt tttcaagaat caaagaagtt cttgacttac caaatttgat tgaaattcaa
    37681 actgactcat tcaaagcttt cctagaccac ggtcttaagg aagtgtttga agatgtattg
    37741 ccaatttcaa acttcacaga cacaatggag ttggaatttg ttggatatga aatcaaggaa
    37801 ccaaaatata cgctagaaga agctcgtatc cacgatgcta gctactcagc accaattttt
    37861 gtaaccttcc gtttgatcaa taaagaaaca agcgaaatca agacccaaga agttttcttt
    37921 ggtgatttcc caatcatgac agaaatgggt actttcatca tcaatggtgg tgaacgtatt
    37981 atcgtttctc agttggtccg ctcaccaggt gtttacttta acgacaaagt agacaaaaat
    38041 ggtaaggtgg gctatggttc aactgttatc cctaaccgtg gagcttggtt ggaacttgaa
    38101 agcgactcaa aagatatcac ctacactcgt atcgaccgta ctcgtaagat tccatttaca
    38161 accttggttc gtgctcttgg tttctcaggt gatgatgaaa tctttgatat ctttggtgac
    38221 agcgaattgg ttcgcaacac tgttgaaaaa gatatccaca agaatccaat ggactctcgt
    38281 acagacgaag ccttgaaaga aatttacgaa cgccttcgtc caggtgagcc taagacggct
    38341 gaaagctcac gtagcttgct tgtagctcgc ttctttgacc cacgtcgtta tgacttggca
    38401 gcagttggtc gttacaaaat caataaaaaa ctcaatgtta aaacacgttt gctcaaccaa
    38461 accattgcag agccattggt agaccctgaa actggagaaa tcttggtaga agctggtacg
    38521 attatgactc gtagcgtgat tgaaagcatt gaaagccatt tggatggcga cttgaacaag
    38581 attgtctaca tcccaaacga tgcagccgtt gtgactgagc ctgttgttct tcaaaaattc
    38641 aaggttattg ctccaactga tccagatcgc gtcgtaacga tcattggtaa tgctaaccca
    38701 gatgacaagg ttcgtacggt gactcctgca gatatccttg ctgagatgag ctacttcctc
    38761 aacttggctg aaggacttgg ccgtgtagat gatatcgacc accttggaaa tcgtcgtatc
    38821 cgtgcggttg gtgaattgct tgccaaccaa gtacgtttgg gactttctcg tatggaacgt
    38881 aatgtccgtg aacgtatgtc tgttcaggac aatgaagtct tgacaccaca acaaattatc
    38941 aatatccgtc ctgtaacagc tgcagttaaa gaattctttg gttcatcaca gttgtcacag
    39001 ttcatggacc aacacaaccc gctttctgag ttgtctcaca aacgccgttt gtcagcctta
    39061 ggacctggtg gtttgactcg tgaccgtgct ggatatgaag tacgtgacgt gcactacact
    39121 cactatggtc gtatgtgtcc aatcgagaca cctgaaggac ctaacatcgg tttgatcaat
    39181 aacttgtcat cttatggaca cttgaacaaa tatggttttg ttcaaacacc ataccgtaag
    39241 gttgaccgtg aaacaggtgt tgtcacgaac gaaattgttt ggttgacagc tgatgaagaa
    39301 gatgaatata ctgtagctca ggctaactct cgtctgaatg aagatggaac ctttgctgag
    39361 aagattgtca tgggacgtca ccaaggggtc aaccaagagt atccagctaa tattgttgac
    39421 tacatggacg tttcaccaaa acaggtagtt gccgttgcga cagcatgtat tcctttcttg
    39481 gaaaacgatg actccaaccg tgccctcatg ggagccaata tgcaacgtca ggctgtgcca
    39541 ttgattaatc ctcaggcacc ttacgttggt actggtatgg aataccaagc agcccacgat
    39601 tctggtgcgg ctgtgattgc tcagtatgat ggtaaagtta cttacgcaga tgctgacaag
    39661 gtagaagttc gtcgtgaaga tggttcattg gatgtttacc acatccaaaa attccgtcgt
    39721 tcaaactcag gtactgctta caaccaacgc actctcgtaa aagttggtga tgtcgttgaa
    39781 aaaggcgatt tcatcgctga cggaccttct atggaaaatg gagaaatggc gcttggacaa
    39841 aacccaatcg ttgcctacat gacttgggaa ggttacaact tcgaggatgc cgttatcatg
    39901 agcgaacgct tggtgaagga cgatgtctac acatctgttc accttgaaga atacgaatca
    39961 gaaacgcgcg atacaaagct tgggcctgaa gaaatcactc gcgaaattcc aaacgttggt
    40021 gaagatgccc tcaaagacct tgacgaaatg gggattatcc gtattggtgc tgaggttaaa
    40081 gaaggtgata ttcttgtagg taaagtaaca cctaagggtg agaaagatct ttcagctgaa
    40141 gaacgtctct tgcacgctat ctttggagac aagtctcgtg aagtgcgtga tacttctctt
    40201 cgtgtaccac acggtgccga tggtgtcgtt cgtgatgtta agatctttac acgtgtaaat
    40261 ggagatgagt tgcaatcagg tgttaacatg ttggttcgtg tttacatcgc tcaaaaacgt
    40321 aagattaagg tcggagataa aatggccgga cgtcacggaa acaaaggggt tgtctctcgt
    40381 atcgttcctg tagaagacat gccttacctt ccagacggaa ctccagtcga catcatgttg
    40441 aacccacttg gggtaccatc acgtatgaat atcggtcagg ttatggagct tcaccttggt
    40501 atggcagctc gtactcttgg tattcacatc gctacaccag tctttgatgg agcaagttct
    40561 gaagatcttt ggtcaactgt taaagaagca ggtatggata gcgatgccaa aacgatcctt
    40621 tacgatggac gtactggtga accgtttgat aaccgtgttt ctgtcggagt catgtacatg
    40681 atcaaactcc accacatggt tgatgataaa ttgcacgcgc gttcagtcgg accatactca
    40741 actgttaccc aacaaccact cggaggtaag gctcagtttg gtggacaacg tttcggtgag
    40801 atggaggttt gggctcttga agcctacggt gcgtcaaatg tccttcaaga aatcttgact
    40861 tacaagtcgg acgatatcaa cggacgtttg aaagcctatg aagctattac aaaaggcaaa
    40921 ccaattccaa aaccaggtgt tccagaatcc ttccgagttc ttgtcaaaga attgcaatct
    40981 cttggtcttg acatgcgtgt cctagacgaa gatgaccaag aagtggaact tcgcgacttg
    41041 gatgaaggaa tggacgaaga tgtcatccac gtagatgacc ttgaaaaagc ccgcgaaaaa
    41101 gcagcccaag aggctaaagc agcctttgaa gctgaagaag ctgagaaagc aacaaaagcg
    41161 gaagcaacag aagaagctgc tgaacaagaa taagcagttc acttagaata gaaagggaag
    41221 aaatagtggt tgatgtaaat cgttttaaaa gtatgcaaat caccctagct tctccaagta
    41281 aagtccgttc atggtcttat ggagaagtca aaaaacctga aacaatcaat tatcgtacct
    41341 tgaaaccaga acgtgaagga ctctttgatg aagtgatctt tggtcctaca aaagactggg
    41401 aatgtgcttg tggtaagtac aaacgcattc gttacagagg aattgtttgt gaccgctgtg
    41461 gggttgaagt aacgcgtacg aaagttcgtc gtgagcgtat gggacatatc gaattgaaag
    41521 ctcctgtatc tcacatctgg tacttcaagg ggattccaag ccgtatgggc ttgacccttg
    41581 atatgagccc tcgtgccctc gaggaagtta tctactttgc ggcttatgtg gtgattgatc
    41641 ctaaggatac accacttgag cacaagtcta tcatgacaga gcgcgaatac cgagagcgct
    41701 tgcgtgaata tggttatggt tcatttgttg ccaagatggg tgcggaagcc atccaagacc
    41761 ttttgaagca agtagatctt gaaaaagaaa ttgctgaact caaagaagaa ttgaaaacag
    41821 ctactggaca aaagcgtgtc aaagccatcc gtcgtttgga tgttttggat gccttttaca
    41881 agtctggaaa caaacctgaa tggatgattc ttaacatcct tccggttatc ccaccagatc
    41941 ttcgtccaat gttgcagttg gatggtggcc gttttgcctc atctgacttg aatgaccttt
    42001 accgccgtgt tatcaaccgt aacaaccgtt tggctcgttt gcttgagtta aatgcaccag
    42061 gtatcatcgt tcaaaatgag aagcgtatgc ttcaagaagc agttgacgct ttgattgaca
    42121 atggtcgtcg tggtcgtcca atcacaggac caggtagccg tccattgaaa tcattgagcc
    42181 acatgcttaa aggtaaacaa ggacgcttcc gtcaaaactt gctcggtaaa cgtgttgact
    42241 tctcaggacg ttccgttatc gccgttggtc caactcttaa gatgtaccaa tgtggtgtgc
    42301 cacgtgaaat ggcgattgaa ctctttaaac catttgtcat gcgtgaaatc gttgcccgtg
    42361 atatcgtgca aaacgtcaaa gcagctaaac gcttggtgga acgcggagat gagcgtatct
    42421 gggatatcct tgaagaagtg attaaagaac acccagtgct tttgaaccgc gcaccgaccc
    42481 ttcaccgttt gggtatccaa gccttcgagc cagtcttaat tgatggtaag gctcttcgct
    42541 tgcacccact tgtctgtgaa gcctacaatg ctgactttga cggggaccaa atggccatcc
    42601 acgtaccact ttcagaagaa gcacaagcag aagctcgtat cctcatgcta gctgctgagc
    42661 acatcttgaa cccgaaagat gggaaaccgg tagttactcc atctcaggac atggttttgg
    42721 gtaactacta cttgaccatg gaagaagctg gtcgcgaagg tgaaggaatg gtcttcaaag
    42781 accgtgacga agcggttatg gcttaccgca atggttatgt tcacctccac tcacgtgttg
    42841 gtatcgcaac agacagcctc aacaagcctt ggacagaaga gcaaagacat aaggtcttgc
    42901 ttacaacagt tggtaaaatt ctcttcaacg atatcatgcc agaggggcta ccatacttgc
    42961 aagaaccaaa caatgccaac ttgacagaag gtgttccagc taaatacttc ttgccacttg
    43021 gtggagatat caaggaagct atcagcaatc ttgagctcaa ccctccattc aagaagaaaa
    43081 accttggaaa tatcatcgct gaaatcttca aacgtttccg tacgacagaa acttctgccc
    43141 tacttgaccg catgaagaac ctcggttacc accactcaac tcttgcagga ttgacagtgg
    43201 gtattgccga tatcccagtc gttgatgaca agactgaaat cattgaagaa tcacacaaac
    43261 gtgtcgaaca aatcaccaaa caattccgtc gtggtatgat cacagacgac gagcgttaca
    43321 atgctgttac agctgaatgg cgtgctgccc gtgaaaaact tgagaaacgc ttgattgcca
    43381 accaagatcc taagaaccca atcgttatga tgatggactc tggagcccgt ggtaacatct
    43441 caaacttctc acagcttgcc ggtatgcgtg gtctgatggc tgctccgaat ggacgtatca
    43501 tggaattgcc aatcctttca aacttccgcg aaggtttgtc ggtattggaa atgttcttct
    43561 caactcacgg tgctcgtaaa ggtatgaccg atacggccct taagacagcc gactcaggtt
    43621 acttgactcg tcgtttggtc gacgttgccc aagacgttat catccgtgag gacgactgtg
    43681 gaactgaccg tggtctcttg atccgttcta tcgcagaagg aaaagagatg atcgagtctc
    43741 tcgaagagcg tctcaacggt cgttacacta agaaaactgt taaacatcca gaaactggtg
    43801 cagtgattat tggtccaaat gaattgatta cagaagacaa ggcgcgtgaa attgtcaatg
    43861 ctggtgtgga agaagtgact atccgttctg tatttacatg taacactcgt cacggtgtct
    43921 gccgtcactg ttacggtatc aacttggcga ctggtgatgc ggttgaagtt ggtgaagcag
    43981 ttggtacaat tgctgcccaa tctatcgggg aacctggtac acagcttaca atgcgtacct
    44041 tccacacagg tggggttgcc tcaaataccg atatcactca gggtcttcct cgtgtccaag
    44101 aaatctttga agcccgtaat cctaaagggg aagcggttat cacagaggtt aaaggacaag
    44161 ttactgctat cgaagaagat gcatcaactc gtaccaagaa agtctttgtt aagggtgaaa
    44221 ctggcgaagg tgaatatgtc gttccattta cagctcgtat gcgtgtcgaa gttgggggcc
    44281 aagtagcgcg tggtgctgct ctgacagaag gttctatcca accaaaacgt ctccttgcag
    44341 ttcgtgatgt cttgtcagtt gaaacgtacc ttctcggtga agtacaaaaa gtttaccgta
    44401 gccaaggggt agaaatcggt gacaaacaca tcgaggtaat ggttcgtcaa atgatccgta
    44461 aagtccgtgt catggatcca ggtgatacag atcttctcat gggtaccctc atggatatca
    44521 atgactttac agatgccaac aaagatgtcc ttatcgcagg tggagttcca gcgacaggtc
    44581 gcccagtcct tatgggaatt accaaagcct cacttgaaac aaacagtttc ttgtcagcag
    44641 cttccttcca agaaacaact cgtgtcctta ctgacgcagc tatccgtggt aagaaagacc
    44701 atctccttgg acttaaagaa aatgttatca tcggtaagat catcccagct ggtactggta
    44761 tggcccgtta ccgtaacctt gaaccacatg ctgtcaacga agaagaatac cttaatcctc
    44821 cagtagagga agaaggaaat gaagaaacaa cagaagtagt tgtggatact gccgttgaaa
    44881 ctgtggaaga aacagtagaa taagagagaa tgagagaacc cgagggttct ctcattctct
    44941 cttctgaaaa ctttctccat ttttccacga aatgtggtaa aataaaagaa agaagctgac
    45001 aaaggagacg ggtatggaac aaacattctt tatcatcaaa ccagatggtg taaaaagagg
    45061 gctagtgggt gaagtgttaa agcgcatcga acaacgtgga tttacaatcg aaaaattgga
    45121 gtttcgttca caggtttcag aagagttgat tgaccagcac tatcaggact tggttggtca
    45181 gagtttttac ctaccgattc gtgaattcat gacttcaggt ccagttcttg tgggtgtcat
    45241 ttctggtccc aaagtaatcg aaacttggcg gaccatgatg ggtgcaactc gtccagaaga
    45301 agctttacca ggcactattc gaggtgactt tgcaaaagct gctggagaaa atgaggttat
    45361 ccaaaatgtt gtacatggtt cagattcaga agagtcagct aagcgagaaa ttgctctttg
    45421 gttttaagag tggattggct caatcaattg gttaaaagct catttgaata gaaagtatag
    45481 tcaattagtt taagacatga cgcatgatat caaacttttt agtttttgat atggtgcgtt
    45541 ttttgattaa gtgactatta gttcgtctcg ctccgttagg tgcgagacaa aaaaacaccc
    45601 gctatgcggg tgcgcgtcga aggttatacc aaaaaaactc caaacgcgat acaataaagg
    45661 tgttcaagcc aattgtaaag cgaaaggaga aaaatatggc acaaaaggtt cttttgtcct
    45721 gttcttgttt caattgacta tatttctata ctttttcttc ctttctctgt gaaaatcgca
    45781 agtttttccc accttttctc aatactgaaa caacatcttt ccagaacttc aataatttct
    45841 ttttgtgtta aatcattatt taactaaatc tttggaggca atacgttttc aaagcgactt
    45901 ttttaccata ggttttgagc cttcaacaca gattactaag ccaaaaatgc tctaaatttt
    45961 tgaaattcta acaaattcca ctcaacttta tcatcatctt tgactttcac aatatcttcc
    46021 tcaaaataac atggaataaa gatagttttc ttttgaaacg gttgtaggag gaaaatctct
    46081 cctacactat tattgattct gagaggaaat tgcaaaatat catctaccaa attttcaatt
    46141 attaagattt tttgcagttt atctgaatta gaatcgcaag gcttctcaat aatataattg
    46201 tcgaattttt tcattctaac ttcctctcca ctatttcctc ctttatcaag aaaaagttac
    46261 ctcaatatta ataaagtaca attctaaatt aactccaatt attgattcat gataatattt
    46321 cagtaactca tcgtgcaatt aaaaacaagg atttttattt cagatgatcc atcgtgaaca
    46381 cttcatcagc catctcccaa attgccggat tatgtgttgc gataatgatt agcctattat
    46441 catctcgaag agatagcaaa atctccataa tcaactgaga ggttgctggg tctattgaag
    46501 ctgttggctc atctgccaga ataaggggtg gattctttaa gataactttt gccaaggcaa
    46561 cccgttgcga ttctccgccc gataactcaa agatgcgctt atctaggtca agataagcca
    46621 ggccgacctg ttctaaagcc tgcttctgcc tcaaccgctg ttccgaccga ctcaactttt
    46681 gaccaatgag acctagctta aggttttctt caatactttg gttttcaatt aagccaaagt
    46741 tctggaagag gtagcccaat tcgtgacgga aaaaatcact cgatttataa ttggccaagt
    46801 ctttacctcg gtaaaaaatc atcccatcat aagattctaa tttcccaatc atgttcatca
    46861 aggttgtttt tccgctacca cttgaaccaa ttaaggcata gacttttcca gcctcaaatg
    46921 tcattgaaag attcgaaaat aactctcgtt ctccaaaaga tttactcacc tgtttaagtt
    46981 caatcatatt aacctccctt caaaacaagc atggacatct tgttttcttt ctgcatttgg
    47041 acatgtaact gtaaaagaga tagaccagta aagagtaacg agactaagaa agcaactagt
    47101 atctctacct gaagaaatac actcgcaaca aatcccagta aaaacacacc cagttgagca
    47161 aagagataag tgcgatggat ttctaagaac ctgagacctg caatgcgttt gataaaaatg
    47221 gcacgtctaa attcttcaaa gtagagccta ttcatagtgt taaacaacaa gattgaagtt
    47281 gcaatcccaa gcacagctcc tgctagcatt acccaacgtt ccctctggat attatccaat
    47341 aatgtgatgt agttgtggta acctgtttgc atttctgaga cccaattttc aatgccttgt
    47401 ctctggataa gctcctgggc atcagacaat tgattaaaga gaacgtagtt ctgtactgcg
    47461 tctatccaaa acaaaatgga ctgtggacca gttaattggg gtgttataac aatgatgatt
    47521 ggatctttca aaaactgctg gtaagaaata ggggtcgtat tatacacaaa acgatcctga
    47581 cctgattcta aataacctac cgtagcagtc atttgctgtc gttcatcttg actagacatg
    47641 cgactggtta agtcgtcttc aaaaacagat ttataatgtt cttcctctga acggaggtgt
    47701 tcaggcagca ataagacaaa ctcccctaca tcaaggtgat tcaacttttg actcatttta
    47761 gttgaactgt aggacttact ttttcagagg gctccaaatt ttattttcat ttactttttt
    47821 tagcccataa aatatttacc atacttataa aaagtagtaa tactccaact aaactgactt
    47881 gtaatgaatt atatgtattt tctgaagtgt taggtaaata attttgagag tacccttctc
    47941 gggcaatagt aataatattg tgtgtgttta tttgtttaat tccaatagta gtttgaattc
    48001 tctcttccaa ctcaattaaa ttaacattat ctatgaaact atcccaactt tgttcatttt
    48061 tatattctaa ttgtaacata ttcaaatcat atctcaatac gtttggtata tttaacactt
    48121 ttataccatt ttttactggt aatgtttctt ccagaatttt ttttacaaac atataattat
    48181 agggctgatc ttttaatttg tatttatcaa taatcattgc aatcgcacca gaaactttag
    48241 gagcagcaaa tgagtttcca taaagatacg tatatcctcc tagtgttgcc gaaaaaatcc
    48301 aatcttcttt atatttttct gcattaataa agttattaag tcctaattct gaaagagata
    48361 atgttgagcc tgcaggcgca tatattgcat cagaatcccc tttattgcta aaatctgata
    48421 aattacctaa gcgatctatg cctccgaccg aaattgtaga tgagaaataa cttggaacat
    48481 caactactaa tcctggtttt cttacttttt tgcgtgaact aataagtttc aataaatctg
    48541 actgatttga tacatttagg gagtcattcc ctaatgcagc tactataatt actccttttt
    48601 gattcgcgta atcaatagcc tttttatact tcaaaaatgt ttcaaaatca tttgttccgt
    48661 cctcatattc tccatcaatc attaaatatt gtccagtact aagattgata atatcattgc
    48721 catcatctac agcatcaaaa attgctttta caatccaatc tggactagct gacgacttac
    48781 caaatattct atagacgtta atattaacgt gaggatttac tccattaata ttgtcatccc
    48841 ctacaatttg agctacaaca gccgtaccat gacctaatct atcttgaata tctgatatta
    48901 ttccttcctc tccctcctct tttcctaaat atcctttatt ggggacatag ttttttgagt
    48961 gatttgaaag atttttcagt aatccagtat tttgtaaatc gactcctgaa tctaaaatag
    49021 caattgaaac tttagaatta cttttagaat ataatttata actttcgcct ccaccagtaa
    49081 ctttatctat atcccattca tattttctta caatcgacga ttctaagtta tctttgtcat
    49141 gctctatatt tcctcctcta ttatctagat gagaaacatt ttccttaatt ttattatcta
    49201 aattatcttc gcctagtaaa ttattacttt cagagttaac aatatcgtta tttatttgtg
    49261 gttcaagttc caatttacaa cagttttttt ctaaatgttt atctgttgtt acaaataatt
    49321 ctgtcttatc gatttgaata cgaaatttat tttggtctac ttcttcttga tttttattag
    49381 aatagtcaac ctgtttttca tcagattcag aaaaactttc gctagtctga gtgtgagtta
    49441 tattgccatc aagattttta ggtatttctt gagcagtaac agaattattg aacaaacaaa
    49501 agaataatat cgctaaagtc caatattttt ttttcataat cttcataaat ttaccctttt
    49561 aatcaaaaac taattatttg aatacaattg cttataaatg cctcctagtt caagaagttc
    49621 tctatgggtc ccaatctcta caatttcacc attatgcatt actaagatct tatctgcaga
    49681 cttgatagta tctagtcgat gggcaattaa aatcttagtg gtaccgattt cacttaagta
    49741 agaagttgta atttgttgag ataaattatc taaactactt gttgcttcgt ccaaaacaac
    49801 taccgaaggt ttcgacaaaa gacaacgagc taaaagtatc atttgctgct gccctccaga
    49861 aaagttatta ccttcttcag cgatgattgt ctctaatcca agaggtaaag atttaactac
    49921 attgctaaga ccaattgaat ttagatttgt cattaattct tcatctgaaa caaagtgtcc
    49981 aaatgtcaaa ttctctctta aggtaccata acttagggtt gaattttgta acactgctcc
    50041 aaaaatcttt ctatacaatg tttgaccaat ttcttctttt tctactccat caacttctat
    50101 tgaaccaata ttaggttcta ataaacctaa taaaatttta gctaatgtac tcttacctga
    50161 acctgattct ccaacaatag caacagtttc tcctttttta atagtaacat ttattccttt
    50221 caaaacataa tcatcaaata atccatattt ataccaaata tcttttaatt ctattttttt
    50281 atcaaatttt atatcttgac tgactctttc tggaattaat tcggatttag tattaaacac
    50341 atcctctatt cttaaaaaat atccctttaa taacattaat tgtatatagt tatcacttaa
    50401 agaaattata ggagaaataa agtatggtga gactgtactt attgctacaa tttgtcctaa
    50461 cgtcaattgt tcgaatgttt ttatatttac acctacaata agggtcaaaa cagggagaat
    50521 aatttgtaaa acattcgtta tactactaac aattgataaa tgtatatcaa gttttcgacc
    50581 tatgatgagc tgttttgtat taaaattatc ccatttgtta atccaaaatt cctcttctcc
    50641 agttagctta atatcactat ttttagaaat tacttcggaa gtaatatttt gaacattaac
    50701 cttttctttt atatttttat caattaaatt ttttgagatt tttatgattg gatacattac
    50761 aatagataga gctagactta gtgatattaa aaagattacc atgtacttag aaaagctaaa
    50821 taaaaccaca gcataaacca caatcattaa caaatcaagt atagctgcta taaaattatt
    50881 tgatagtatt tctcttatat aaacaataga gttagctcta aaaagtatat cccctaaagt
    50941 acgtttttca taaaaactgt aaggtaaata tatcaaatgt ttcataaaat catagctaat
    51001 ctctttatcc attatatatt ttaaggatgc aacatatatc tgtcttaata aatacattag
    51061 tgagaatgaa acagtaaata taaataatat agtaaataac attctagacg aatacgaatc
    51121 gtccttgaaa ttcgtgtcaa ttatgtatct attagctata ggtactaatg attgtattac
    51181 atacaataat gctgttacaa aaaataaaat ccctagctta ttcctatact taaaaaaata
    51241 ctttaaaaca ggcgacttct tattatcttt acgagacata aagctatcta actttttaaa
    51301 tgttaaaatg atatttgaat aatgcctttc aaattcactt attgacattc tctgcctgcc
    51361 ttttgcagga tctaaaatat aaaacttgtt tttactaatt ttttcaagaa taataaaatg
    51421 agaactctcc cagaagcaaa tgagtgggaa actgagcggt tttaaatctc ttaccgaagc
    51481 tttaaaagca tgagaactta tattaaaatc tcctaaaact ttacttatag ataggagaga
    51541 atatccagat ctggatgatg ggtagatttc acgtagttca tttaaagaaa tctcatattt
    51601 aaaatagttc aatatcatgg cacagcatgc taatccacat tctgaatttt ccatttgata
    51661 tatcattgga attttcacaa tacttcctcc ttgttcaaaa taaagtcgat aatttcttct
    51721 tgtctaaaat ctactaattt ctttagatac ttaatctttt ctaaatattt attatttttc
    51781 gagtaaatat agtttgacaa ggtaaaaaca acttcttcta aagataagtt ctgtggaata
    51841 atccaatcag aagatttatg tagtaattta tagtactcat ggataatatt ttcctgaata
    51901 tttgaatcaa aaacttcatg attagcaatt gtaaatgctg acccttcacc tttcacttca
    51961 aagtaaaggt ttatcaaaca tttttcaaat tttgaaaaaa aagatttttc tttagaggtt
    52021 aattttgcaa aatcgtcgat tgttatataa ggattattat agagactgtt cgcaaagaat
    52081 ctctgatatg tttttgaatc ttttgaatac aaaactatct ctctaatagc attgccatct
    52141 gttccatcaa ttggtaaaca taccgtaact agaaaaagaa ttatattcaa aataaaaaat
    52201 tctgatgcgt acggcacaaa tcccaaaagt gctaatattg cgacaattag gttagctcca
    52261 cctcccccaa agaagtagaa caccaaattc ctatcactat ttttttcatt agtaatgttt
    52321 ctattactca tttgacaata accgaatgct aataacactg gaaatttgaa atatattttt
    52381 tttctgaaat agaagaaaaa gggagtagca agcatctcta gtttataaga taaacatctt
    52441 cccactaaaa aatgacctag ttcatgtaat gtaattgata ttaacgaaat taaaatcaat
    52501 cgaaaataat agattaatga atcatttgga aaaattatca ataataggaa caataacgga
    52561 atcaaacata aatatatgac agagttattt aatattttca acataatacc attcctctaa
    52621 actattagct tcaaaaaggc gttttttctc ccaatacatc ttctcaaaat gttcggaatc
    52681 ataattttct aaaattaatt ttaatgtctg gtaagctctt tcttgataat ccgttgtttt
    52741 gtacttaatt ttcccttcaa gtacatcttc aattttataa gttgcctcca tcaactgagc
    52801 ctctgcaata tctttgagtg aattggtaat tgaaacttgg tgtaatatct gtcctcccat
    52861 atatgaaaat atatctctaa gatattctga cacattatca gagccgttac tctcagcaac
    52921 atctaatgtt acaacaaact ttccagctaa tcgaaaaaga tggctccacc ccccaatcct
    52981 ttcaataaag ttttttgtgt ccacagatac gttttgtaaa tatacaggag aagagataat
    53041 tataatatca gactctaata actctttttt tataacacct ccatcatcag cattactttg
    53101 cctatcaatt cctttcttaa acaactcttc tgaatcagaa ttagatattt ctagctctga
    53161 attgaaaggt gtcctgaaag atatatcaac attatttcta ctagaaatga tacttgaaag
    53221 tctcttagta tactctaaag tcttagagtt atgatttcgc actcctgcat atataaatat
    53281 tttattcatt ttaattcatc ctctcaattt gaatttagta gatttttcaa gatagtatgg
    53341 tacaaaaaca gacttttgtt gactcacatt attacatatg ttttgtatta aaccaaaatc
    53401 aatactattt ttggagtaat tttgatttta gtttaaaatc atttctataa cagtagcata
    53461 tacctcaagc cgtttagcaa ttagaataga acctttcttt attatattat tatctcaacg
    53521 aaaagctaca ctattaaaaa tattttatag aattacatat taaactagtc aatcttggta
    53581 tttttatatt gcttaatgag tggacacctc tattttagaa acaaaactat aaattaagct
    53641 agatttcaag taatgagggg ataactatct ttttgtcatt ctgattcagt gcgatatacc
    53701 ttaaaaaagt ataagcaata ccagtcacac ctgtatacaa agaaaaatct gggaaattgc
    53761 ttgtttggac gatacgatac tctccttctt ttgatttatt cattacaaca ctacacaata
    53821 aagactccaa ttccatacta gtatccattt ctttcatgta gtcgatgtaa aaatttatta
    53881 tggccatact tccatggcaa aatgtatcat tatctaaact agctacaatt ccctctggaa
    53941 cactttgggg atgattaact aatgtcccaa attctccact acaccacttc aaagaatgaa
    54001 ttttgatttt ctccctagga actagttgta aaattaattc tttatatttt ttaagtcttg
    54061 tcactttata aatatttttt aatgtaaaaa ttacacctga tagtccatgg ccaaaactat
    54121 atccaaaatt actattatct ctctcgctta catcattata tagcgtatca cctaaactta
    54181 atactagcct tagaacacgt tccttctcta ttcctctcct ataatatctt actagtgtat
    54241 taattaaagg tagaagacca ttaatatagt cagacttgtt tgaaacactt gcaaaatcag
    54301 tcttttcaag ctcagttaaa acactcttta tataatttaa gcatgcgaga gtatttgtat
    54361 cgtaatcctc tataatggat agaacaatga aatatcctat atccccagtt aaaccaaatg
    54421 tggtcttaga taaagaaaca gatggcggaa ttgcagataa cattttattg tacagttgag
    54481 tatatgatga tttatctttc aataatttta catagtacat aaacagtaat attccagctc
    54541 tacccctata catatcatta cccgtttgtt caagacacca tttagaacct ttaaaattaa
    54601 caggtatact ccaaattgga tattcgtcat aaatattatt aataaccaaa gagtctgcaa
    54661 tattttctac ttcattatgc agaatagtaa ctaaactttc atttgggagt ttttttctat
    54721 tagataagtt taatttatat cctttttttc gctgatcaaa gcttggaaaa taaatttcaa
    54781 tgatatcaag ttgcttttct aaattttcca aattattatt aggtaaatat ttcataaaat
    54841 agtcatatcc agaaaattga tgtagggaaa taaaatgatt tccaaaatca tcgtagattt
    54901 cattgatatt tgtatctgta taaaaaatcg gaatatctaa taacctcatt tgttcacatt
    54961 cgcttgctac aataccttga ttagaaaact tattgctcca gagattttcc aatgcttttt
    55021 ctctatctaa catttcttca taaaaatcag gatgatataa aaaagatagt actgaagcat
    55081 agctatttgt gtctctaaaa agtacccttg tctttaaacc atacaagttt gcttttaata
    55141 gcattttaaa ttcttctgtt ttatttaact cttcaaatat cagataaaaa tccctaaaac
    55201 cttttttgaa atcttttata tacttatcaa attctatatc accatcccga acaggcaggt
    55261 ttttcccacc ttcaaaatca attttcccaa tatcaaactt taccttatca gtatttaaat
    55321 taattaaaac ttgaccatta aaagcatcct tcttaaaatt tcctgtaaga gcactcatct
    55381 caacatcaag accatcctcc ctttttactc cctctgttgg aagaagcaaa gttcgagtca
    55441 ccctgtgaaa cagggtatcc agcaaactct gaccagtttt atcatcaaat agaggttgct
    55501 gtaacattgt ttcaaagtct attattacag gcatatctcc ataggaaatt aaattttcaa
    55561 aatgtatatc actcccatta aataaatata taaatgctaa aagcacacca aaattataat
    55621 aatattcagg taattttttc ttttgctcta gaggtagata atcaataaat tcttcatagc
    55681 tgtgatccga ataagcaata gttcgaggta ttcttatcct aattttttca tcaacctttc
    55741 ggatatactc agcaatagta tttagtgagt tattgattat taaattttta ggcttgtaaa
    55801 caattttcgc gtcatcaaag aagagtatca aaacagtttt tccttggctg tgtgtatcac
    55861 cctttccaaa ttttatatca tttaatttcc cccttagccc taattcttct tctagcacac
    55921 taaaatcatt ttctaaattt gataaaattt ctataaaata agtatagaac cgaagacaag
    55981 actccgatat ttgcctcagt aaaacgggat atttgttata aaaatttaat atattctctt
    56041 ccttgtcaaa caatgttata aaatatttgt accgttgctg ctccgattcc ccttcaagta
    56101 aacctttttc acgcaatgaa tttatctcta aaaccaaagg tttaacaatc caaggataga
    56161 ttaaatcttc taaataaaat actagagagg aaaaaatatt tccattcttg tcaatccact
    56221 cgtattcttt attctctaga taattattga ttaatttaag caaattaata ataaagggtt
    56281 ccaaaattct agaaaaatct ttcttaaaat taattctttg gggatgtttt ctaaatattt
    56341 caaatgaacc ctccgcaaat ttataccatt ccgaattttt ggcatattct gaatatagtc
    56401 ttatctgttc ctcggtataa tcatagttca cagtgttaga aaatattttt ttattgtaat
    56461 tatttaattt caacatttct tcaaaagttt tttcagaaat aattgacatt gtagaacgcc
    56521 aattatttaa tgaaaagtta tttaaggaat tatcatttcg ttcagataat atttgatatc
    56581 tttccttaat aaacaatgtt ttatgcacat ttttaatttt atatttcatt tacttacctc
    56641 aaacctatac gacttactat tgttttaaaa aaaaaacctc tctcaaaatt attagttacg
    56701 agagaggaaa ataaagagca acttttattt ttaaaattga atattttatt ttttcttgtt
    56761 gtctttgcca caacgagtct gagctgaccc caaatatgat gcaataaaac ttaatgtagc
    56821 agaacatata attgttgatc tagggtcagc accatctgtg ccaccataaa ccaattgcat
    56881 ttcttcaaga gataattctt tcaaactctt tccgattaca aaatcatttt tcattttaat
    56941 ttaccccaaa caatcattat tgtagctaac tagcccccca ataccagaaa cagctgcact
    57001 aatgcatact ttcgagctag ctgcagctga tttcaaaata ataggagtag gcgtccaacg
    57061 tggctctgca ccatcagatg caccataaat taaactcatt tcatcagcag acaatgattt
    57121 taaacttttt ccacaaaatt tttcgtttga attaaagttg gtcataagta ataactcctc
    57181 cttataaaaa gttaaataag ttgctctata tttttcgttt tttcagataa attataattc
    57241 tctcattctc gtgcaaaata tcgggtttta atccgccttc ccaacatcaa gaccattgga
    57301 cttcttttgc gattgcactc gaatctccaa ttgtttattc ttctgaataa actgttggta
    57361 aggaattgta ttactaaaca taaaaacacc tagcaatgca aatgtaacta attttttaat
    57421 tccacgtttt tttctcatgc aatgaaaccc ctttcttttt ccttgttttt attctaacag
    57481 atatatttta tttagtcaag tatttttatg taatttttaa cttttttttt tgtttattac
    57541 cataaagaat tatttattcc tttaacttgc tattttgaac taaaaacttt ataatgaaat
    57601 taagcaaata ggaaggttta ttatctttaa tgaatacact cgcagagaaa ttcagattga
    57661 aaagaaaaga gttgagactc tcccaacaaa ctcttgcaga aggaatttgt gaacaaagcc
    57721 agattagtaa aattgaaaga ggtcatttta ttccctccgc agacttgtta ttcaaactct
    57781 ctcaacgact tgaagtccct ttggattact ttttcaatga acaaatcgaa gttaagtcta
    57841 acctctctaa tttcaaacat ctatctgctc gcctattaga tgacagaaat tatgacgatt
    57901 tagaatatct ttataaaata gaggtagaac gaagcacttt tctaacatta gaagaccgaa
    57961 cttaccttga atggattaaa gctattattg acttctatca atatgacagt aagtgtgagg
    58021 caatttcttc attggaaaat atattattaa aagtctcttc aaatactctg atttatttaa
    58081 aggcgttgaa tactctatct aatttctatt ccttagtggg tcgtgaacaa gaatatgagg
    58141 caaactactc tcatttaata gagttgtatc agacaaaaaa tttggaccat caagagtttt
    58201 tatttggcta catcagagtt cgttacaact acgctcacta cctagtatca aaggaaaaat
    58261 ataatgaagc catccaagaa gctcttgaaa cgattgaact ctgtaaacaa agacagacaa
    58321 gctaccaatt ggctcctcta cttattcttg taggaaatgc tggagccaaa tttctagaca
    58381 gagaacaagt caaaaattat tatatagaag caagagagtt atgtaagatt tataacaatc
    58441 ctttaatgtt gatgaagata gaaaattatt tgaaggaatt agatactgtt tagttaatct
    58501 tgacattata gtttttctga aacgttaaat aacctgtaag gctgatagtt agattaatac
    58561 tatcagcctt ataattatgt gactctgagt ccgtctcgct ccgtgaggtg cgagacaaaa
    58621 caaccacccg tttgacggat tttcatgatt tcaaatttat attcttcgaa aatctcttca
    58681 aatcacgtca gctctatctg caatctcaaa acagtgtttt gagcaacctg tggctagcta
    58741 cctagtttga tctttgattt tcattgagta ttagtctcta gtgtttgttt agcgtcttaa
    58801 agagacattt gagaagatta gaaacagttc tctcttttct cctagtataa tgattggtag
    58861 gaagggagag agaagatgaa atcaatgaga atcttatttt tgttagcttt aattcaaatc
    58921 agtttgagta gctgtttcct atggaaggaa tgcatcttgt cctttaaaca aagtacagtt
    58981 tttttcatcg gaagcatggt tttcgtttca ggaatctgtg ctggagtaaa ttatctttat
    59041 acccgtaagc aagaagtcca tagtgtccta gccagtaaga agtctgtgaa gcttttttac
    59101 agtatgttac tcttaattaa tttgttagga gctgttcttg ttttgtcaga taacttgttc
    59161 atcaaaaata cgctgcagca agaattagtt gactttttat tgccatcctt ctttttccta
    59221 tttgggctag atttgctgat ttttttaccc ttgaaaaaat acgtgcgcga ttttcttgct
    59281 atgctagaca gaaaaaagac agtgttggtg actattttag caacacttct tttcttaaga
    59341 aatccaatga ccattgtctc acttctgatt tatattggac tgggcttgtt ttttgcagcc
    59401 tatcttgtcc caaattcggt taagaaggaa gtttcctttt atggtcatat tttccgagat
    59461 cttgtattgg tcattgttac gctcattttc ttttagagaa agcctgtttt ttatggatgt
    59521 tatgatgata agtttagtag gaaaatagac acaaaagaaa agtttttggt ataataagaa
    59581 tatgtacaca aaaaatgaag aagagttgca agccttaggg gagcgtttgg gccatctatt
    59641 agcaaagaat gatgttttaa tcttaactgg agaactgggt gcaggtaaaa cgacctttac
    59701 taaaggactt gcaaaaggat tacagatttc tcaaatgatt aaaagtccca cctatactat
    59761 cgtgagagag tatgaaggtc gacttccact ttatcaccta gatgtttatc gtattgaagg
    59821 agatgctgat tctatcgact tggatgagtt tatctttggt ggcggtgtga ctgttattga
    59881 gtggggaaat ctcttaggag atgccttgcc agatacttat ttagaattgg aaattctaaa
    59941 agaagcagat ggacgccgtt taaattttca ggcaaagggt ttgcgtgctg agaaattgtt
    60001 agaggagctt caatatggag tatgaattgc tcattaggga agcagagccc aaagatgcag
    60061 ctgaattagt ggccttttta aatcgtgtga gtttggagac agactttacc agcctagacg
    60121 gagatggtat tctcttgact agtgaggaga tggaaatatt cctcaacaag caagctagtt
    60181 cggacaatca gataacctta cttgcattct taaatggcaa aattgctggt attgtaaata
    60241 ttacagctga ccagcgtaag agagtccgtc atattgggga tctcttcatt gtgattggaa
    60301 aaagatattg gaataatggc ttgggaagtt tgttgctaga agaagcgata gagtgggcac
    60361 aagcaggtgg cattctgcgt cgtctccaac tgactgtcca aactcgtaat caagcagcag
    60421 tccatcttta tcaaaagcat ggctttgtca ttgaaggtag ccaagagcgt ggggcatata
    60481 tagaagaagg gaaatttatc gatgtttact tgatgggtaa actgataggt tagtagaaat
    60541 atggttaaaa aaattattgg aatggtgcta gctttacttt ctgtaactgt agtaggagta
    60601 ggtgtttttg cttatactat ttatcaacaa gggacagaaa ccttagctaa aacctataaa
    60661 aaaatcggcg aagaaaccaa ggttattgaa gcgactgaac ctctaaccat tctgttaatg
    60721 ggagtggaca ccggaaatgt tgaacgaact gaaacttggg tcggtagaag tgatagcatg
    60781 atcttgatga cagtgaatcc taaaacgaaa aaaacaacaa tgatgagttt agagcgggat
    60841 attctgacgc gcattgaatc agggaatggt caggctcatg aagcgaaact gaactcagca
    60901 tatgcagatg gtggagcaga gcttgctata gaaaccattc aaaaaatgat gaatatccat
    60961 attgatcgct atgtgatggt caatatgaga gggttgcaaa aattagtgga tgcagtagga
    61021 ggtattacag tcaataatat cctaggtttc ccaatttcta tcagtgacca agaagaattt
    61081 aataccattt ctatcggtgt tggggagcaa catattgggg gagaagaagc cctagtctat
    61141 gcacgaatgc gttaccaaga tcctgagggg gattatggtc gtcaaaaacg tcaacgtgaa
    61201 gttattcaaa aagtcatgga aaaagctctc agtttaaata gcgttggtca ttatcaagag
    61261 attctaaaag ctttgagtga caatatgcag accaatattg atttgtctgc aaaaagtatc
    61321 cctaacttgc taggctataa agattcattt aaaaccattg aaactcagca gttgcagggt
    61381 gaaggagaga tacttcaagg tgtttcttac cagattgttt cgagagcaca tatgttggaa
    61441 atgcaaaata tactccgacg ttctttggga caagaagaag ttactcagct tgaaaccaat
    61501 gcggttttat ttgaagattt atttggcaga gcacctgttg gtgatgaaga taattaactt
    61561 ttgatacaaa taaaaaaatc aatcgtagga aaattcctgc gattttttca aaaaaatacg
    61621 aatagatagg taggaggaaa catgaaagca gaaatcattg ctgttggaac agagattttg
    61681 acaggacaga ttgtcaacac caatgcccag tttttgtcgg aaaaactagc tgagattggg
    61741 gtagatgtat attttcagac ggctgtagga gacaatgaag ttcgtctctt gtctttgctt
    61801 gagattgcca gtcaacgtag cagtctggtg attttgacag gcggtttggg gccaactgag
    61861 gacgacctaa ccaaacaaac cctagctaaa tttttaggga aagcattagt ctttgatcct
    61921 caggctcagg agaagttgga tatctttttt gccctgcgac cagactatgc ccgaacaccg
    61981 aataacgaaa gacaagctca aattgtagaa ggagcgattc cactgccaaa cgaaacagga
    62041 ctggctgttg gaggaaaatt agaagtagac ggagtgacct atgtcgtcct tccaggtccg
    62101 ccaagtgaat tgaaacccat ggtcttaaac caacttctac ccaagttgat gacagggagc
    62161 aagctgtatt cccgagttct tcgtttcttt gggattggcg agagccagct ggttacgatt
    62221 ttggctgatt taattgataa tcagatcgat cctaccttgg ccccttatgc caagacagga
    62281 gaagtcactc tacgtctgtc aacaaaggct agcagtcaag aagaggcgaa tcaagcgctg
    62341 gatatcttgg aaaatcaaat cttggactgc cagactttcg aaggaatttc tttacgagac
    62401 ttttgctatg gttatgggga agaaactagt ttagccagca ttgtggtaga agaactgaaa
    62461 aggcaaggga aaaccatcgc ggctgcagag agtttaacgg caggtctttt ccaagctacc
    62521 gtggcgaatt tttctggagc ttcaagtata tttaagggtg gttttgtgac ctatagcttg
    62581 gaggaaaaat caaggatgtt ggatattcct gccaagaatt tggaagaaca tggtgtggtg
    62641 tctgaattta cagctcagaa gatggctgag caggcacgaa gcaagaccca gtctgatttt
    62701 ggaattagtt tgaccggagt ggcaggacca gatagcctag aaggacaccc agttgggaca
    62761 gtcttcatag gcttggcgca agagcaagga actgaggtta tcaaggttaa tattggaggc
    62821 agaagccgag cagatgtacg tcatattgcg gttatgcatg cctttaacct agttcgcaag
    62881 gctttattaa gtgactaact tttgatataa tagtagatag gtctgaggat cattagaatg
    62941 taggagaata gaatggcgaa aaaaccaaaa aaattagaag aaatttcaaa aaaatttggg
    63001 gcagaacgtg aaaaggcctt gaatgatgct cttaaattga ttgagaaaga ctttggtaaa
    63061 ggatcaatca tgcgtttggg tgaacgtgcg gagcaaaagg tgcaagtgat gagctcaggt
    63121 tctttagctc ttgacattgc ccttggctca ggtggttatc ctaagggacg tatcatcgaa
    63181 atctatggcc cagagtcatc tggtaagaca acggttgccc ttcatgcagt tgcacaagcg
    63241 caaaaagaag gtgggattgc tgcctttatc gatgcggaac atgcccttga tccagcttat
    63301 gctgcggccc ttggtgtcaa tattgacgaa ttgctcttgt ctcaaccaga ctcaggagag
    63361 caaggtcttg agattgcggg aaaattgatt gactcaggtg cagttgatct tgtcgtagtc
    63421 gactcagttg ctgcccttgt tcctcgtgcg gaaattgatg gagatatcgg agatagccat
    63481 gttggtttgc aggctcgtat gatgagccag gccatgcgta aacttggcgc ctctatcaat
    63541 aaaaccaaaa caattgccat ttttatcaac caattgcgtg aaaaagttgg agtgatgttt
    63601 ggaaatccag aaacaacacc gggcggacgt gctttgaaat tctatgcttc agtccgcttg
    63661 gatgttcgtg gtaatacaca aattaaggga actggtgacc aaaaagaaac caatgtcggt
    63721 aaagaaacta agattaaggt tgtaaaaaat aaggtagctc caccgtttaa ggaagccgta
    63781 gttgaaatta tgtacggaga aggaatttct aagactggtg agcttttgaa gattgcaagc
    63841 gatttggata ttatcaaaaa agcaggggct tggtattctt acaaagatga aaaaattggg
    63901 caaggttctg agaatgctaa gaaatacttg gcagagcacc cagaaatctt tgatgaaatt
    63961 gataagcaag tccgttctaa atttggcttg attgatggag aagaagtttc agaacaagat
    64021 actgaaaaca aaaaagatga gccaaagaaa gaagaagcag tgaatgaaga agttacgctt
    64081 gacttaggcg atgaacttga aatcgaaatt gaagaataag ctgttaaagc agtggagaaa
    64141 tccgctactt tttcgatttt tgattcaagt ttttagatta tatatagtag cttgaaataa
    64201 gatatgaaca actctattag gaaagtcaaa ttaatttcta gaaatgtttt agcagctaca
    64261 gcgtactatt ccaaactcaa ccaactataa tagatcgaaa ctagaatagt acatatctac
    64321 ttctaaaaca ttgttaaaaa tcgatttgac tttccttatt tcattccgct atatataagt
    64381 ttgctgtttc ttgtcgctcc tctggaaagc tgatataata gctttatgaa taaaaaacga
    64441 acagtggacc tgatacatgg tccgattctt ccctcgctct taagcttcac ctttccaatt
    64501 ttgctatcaa atatttttca acagctctat aacactgctg atgtcttgat tgttggacga
    64561 tttcttggtc aagaatcctt ggctgcagta ggagcgacga cagcgatttt tgacctgatt
    64621 gtaggtttta cacttggtgt tggcaatggc atggggattg tcattgctcg ttattatggg
    64681 gctcggaatt tcactaaaat caaggaagca gtagcagcta cttggatttt aggtgctctt
    64741 ttgagcattc tagttatgtt gctgggcttt cttggcttgt atcctctctt gcaatactta
    64801 gatactcctg cagaaattct tcctcaatct tatcaatata tttctatgat tgtgacctgt
    64861 gtaggtgtca gctttgctta taatcttttt gcaggcttgt tgcggtctat tggtgacagt
    64921 ctagcagccc tgggatttct gattttctct gccttggtta atgtggttct ggatctctat
    64981 tttattacgc aattgcatct gggagttcaa tccgcaggac ttgctaccat tatttcgcaa
    65041 ggtttatcag cggttctctg cttttattat attcgtaaaa gtgtgccaga actcttgcca
    65101 cagtttaaac atttcaaatg ggacaaaagc ttgtacgcag atctcttgga gcaaggtttg
    65161 gctatgggct tgatgagttc aattgtatct atcggcagtg tgattttaca gtcttctgtt
    65221 aatacatttg gtgcagtgat tattagtgcc cagacggcag ctcgacgcat tatgaccttt
    65281 gcccttcttc ctatgaccgc tatttctgca tcaatgacga cctttgcttc tcagaatcta
    65341 ggagctaagc aacctgaccg cattgttcaa ggtcttcgaa tcggcagtcg tttaagtata
    65401 tcctgggcag tttttgtttg tattttcctc ttttttgcca gtccagcttt ggtttccttc
    65461 ttggctagtt cgacagatgg ttacttgata gaaaatggaa gtctctatct gcaaatcagt
    65521 tcaacctttt atcccatttt gagcctcttg ttgatttatc gcaattgctt gcagggcttg
    65581 gggcaaaaga tccttcctct agtttctagc tttattgaac taatcggaaa aatcgttttt
    65641 gtggttttga ttattccttg ggcaggatat aagggtgtta tcctttgtga acctcttatc
    65701 tgggttgcca tgacagttca actgtacttc tcattattcc gtcatccctt gataaaagaa
    65761 ggcaaggcaa tcttggcaac caaagtgcaa tcctagttgg atttactgaa taaaatccat
    65821 ttcctctagt gaaaatcgaa aaaacttgtg ttctcgtctt tagtttggtg ttgaaaatag
    65881 tttaacagac ttttgacttc ttttatatga tataataaag tatagtattt atgaaaagga
    65941 catatagaga ctgtaaaaat atacttttga aaagcttttt agtctggggt gttattgtag
    66001 atagaatgca gaccttgtca gtcctattta cagtgtcaaa atagtgcgtt ttgaagttct
    66061 atctacaagc ctaatcgtga ctaagattgt cttctttgta aggtagaaat aaaggagttt
    66121 ctggttctgg attgtaaaaa atgagttgtt ttaattgata aggagtagaa tatggaaatt
    66181 aatgtgagta aattaagaac agatttgcct caagtcggcg tgcaaccata taggcaagta
    66241 cacgcacact caactgggaa tccgcattca accgtacaga atgaagcgga ttatcactgg
    66301 cggaaagacc cagaattagg ttttttctcg cacattgttg ggaacggttg catcatgcag
    66361 gtaggacctg ttgataatgg tgcctgggac gttgggggcg gttggaatgc tgagacctat
    66421 gcagcggttg aacttattga aagccattca accaaagaag agttcatgac ggactaccgc
    66481 ctttatatcg aactcttacg caatctagca gatgaagcag gtttgccgaa aacgcttgat
    66541 acagggagtt tagctggaat taaaacgcac gagtattgca cgaataacca accaaacaac
    66601 cactcagacc acgttgaccc ttatccatat cttgctaaat ggggcattag ccgtgagcag
    66661 tttaagcatg atattgagaa cggcttgacg attgaaacag gctggcagaa gaatgacact
    66721 ggctactggt acgtacattc agacggctct tatccaaaag acaagtttga gaaaatcaat
    66781 ggcacttggt actactttga cagttcaggc tatatgcttg cagaccgctg gaggaagcac
    66841 acagacggca actggtactg gttcgacaac tcaggcgaaa tggctacagg ctggaagaaa
    66901 atcgctgata agtggtacta tttcaacgaa gaaggtgcca tgaagacagg ctgggtcaag
    66961 tacaaggaca cttggtacta cttagacgct aaagaaggcg ccatggtatc aaatgccttt
    67021 atccagtcag cggacggaac aggctggtac tacctcaaac cagacggaac actggcagac
    67081 aagccagaat tcacagtaga gccagatggc ttgattacag taaaataata atggaatgtc
    67141 tttcaaatca gaacagcgca tattattagg tcttgaaaaa gcttaatagt atgcgttttc
    67201 ttgtggagat atttccttca atttttgtta ctatattaaa caaaaatcaa aaagcaaact
    67261 agaaagttat gctcaaataa aatctaaatt tgacaatgta aaccgagtcg gatagcttta
    67321 agtactgttt tgaggttgaa gatacgattt ttgataggaa ctcatcaaat aggtttaata
    67381 tcatcaattt tagattttta agtagcatca ataaattgct tccttgtttt gtcataattt
    67441 ttttatttaa aaaattatga caagaatgtg ctattctttt tatgagaggt gtatgaatat
    67501 gataaatgta tgtgatgttg gaaaaagaat aaaagaactt agaatatctt caaatcttac
    67561 tcaagataag attgctgagt atttgtcttt gaatcaaagc atgattgcca aaatggaaaa
    67621 aggtgaaagg aatatcacga atggatttaa gtaataaagc ttcaaatctt agaaaaaagt
    67681 tgggagctga tggtgaatcg ccgatagata tttttaaatt ggtacaaaag atagaaaatt
    67741 tgacgctggt attttatgga ctcggaaaga atattagcgg agtctgttat aaaggaactc
    67801 agttcagtct cattgcagtc aattcagaca tgccattagg aaggtaaaga ttttctttag
    67861 cacatggact gtatcatctt tattatgatg aggtgaagaa gagttcagtc agtcttatct
    67921 tgattggtga aggagatgaa actgaaagaa aagcggatca gtttgcttct tattttttaa
    67981 ttttcccatc ttcactgtat aggatggttg aggaaatcag agaaaatgcc aatagaactc
    68041 atcttgaagt agaagatatt ataaaattgg gtcagtttta tggtatcagt cataaagcta
    68101 tgttatatag attgagaaat gatggatacc ttgatgcaga agaaattaaa aatatggata
    68161 ttagtgttat agagacagct tcaagattag gctatgatac aagtttatat cgtcctttgt
    68221 cagaaagtaa aaaggaaatg gtattaggac actatattaa atcgactgaa caacttttag
    68281 aaaataacag aatttcgcaa gggaagtatg aggaactgtt actagatgct ttcagatatg
    68341 atattgtata tgggctagat gaagaggggg agttgtcgtt tgactagtcg tgtatttatt
    68401 gatgcagatt gtatttcagc atttttatgg gttggcactg aacatctttt agaaaagctc
    68461 tattcgggta aaattgttat tccacaagag gtgtatgatg aaatcaatat acctacaatt
    68521 ccccatttaa aatctaggat agaccagttg gtaactaagg gttcagctga gattgtgagc
    68581 atagacattg gaactgaaga atacgcatta tatagagatt taacaagaaa ttatgatagt
    68641 aacaagatta ttggtaaggg agaagcgact tctattttct tagcgaaaaa gcataatggg
    68701 atattaggaa gtaataacct aagagatgtt aaaccatatg tagaagaatt ttctttagaa
    68761 catatgacaa caggagatat actgattgaa gcgtttaaag cgtaatttat tactgaacaa
    68821 gagggcaatc atatctggaa taatatgctt aaaaagagaa ggaaaattgg tgcaaattca
    68881 ttttcagact atcttcgtgg aagtattcat caaaatagac aaaaataaat ttggataaat
    68941 cgaactcact attcaggagg catatgagca attcgaaaaa gaaaagtgtc aaattgagcc
    69001 tataggagta gaagtgaaat agtaagtcct gcatagtgga tgagagaaaa gttctccttg
    69061 aagttttcct gaactatcag tcgcatgtca aacgatatgt agggtaatgt gagaggggat
    69121 agcgagtagt ttttggttat tttatcaaaa aacttatatt ttattatacc gaatgataaa
    69181 atataataaa aatgatagaa taaggaaaaa acatgaatgt caaaaagata atgtcaattt
    69241 ttcaatcctt ttatgttgat gtcagtattg aggaactgac tttgacttta ccaatcagtt
    69301 ttgtaaaaag gtttgagtat actcaaatga cttttcataa ggaatcattt ttattgatta
    69361 aagaaaagag aagggggagt ttgagttcat ttgttactca ggctcgcact atgggtgaaa
    69421 aagccaatat ggatgttgtt ttggtgtttt cgaagttatc agacagtgaa aaaaagcaat
    69481 tacttcaagc tagagttccg tttgtagact ttaagggaaa cctcttcttc cctccattgg
    69541 gactagtact caatgcgaat gatactgaaa tccctaagga attaacacct agcgaacaat
    69601 taacgtggat tgccttttta ttgccagatg gcgatataaa gcagataaaa tctgtttcta
    69661 accttctata tggtggtgct tatgctttgt cgcattcaac ttttttagct gaaacggatg
    69721 aaaatactag ctatgtcata tggcagagaa aattcaatca gttatccttg ccactttctc
    69781 agcatgtttt aaaaggaaag atgctagaga tatggaaata tcgtcctttt gtatctgagt
    69841 tttggaatga ttttaaaaat aatcatgata aacaatttgt agatccgatt tctctttatt
    69901 tgaccttaaa agatgatgat gacccacgta tagaggaaga gagtgaagca ctagaaaata
    69961 tgatattaca gtatctggga gaagatgatg ccagttaata cgaaagttat ttttcaagaa
    70021 atgtttgcgg attttcagaa ctattatgtt ctgattgggg gaactgctac ctctatcgta
    70081 ttggattcgc aaggatttaa aagtcgcaca acaaaagatt atgatatggt catcatttat
    70141 gaagtaaaaa ataaggaatt ttatactacc ttgaatcatt ttttagaatt gggagagtat
    70201 caaggaagtc agaaagatga gaaagcgcag ctttttcgat ttacaacaac taatcctgag
    70261 tttccttcta tgattgaact atttagtatc ttaccagaat atccattaaa gaaggacggt
    70321 cgagaaattc ccttacattt tgaccaagat gctagtttat cagccttatt attggatgaa
    70381 gattattata atatattggt gcatgaaaaa gaaaccattc aggggtattc ggtattgagt
    70441 aattgtggtt tatactcttc gaaaatctct tcaaaccacg tcagcttcca tctacaacct
    70501 caaaacagtg ttttgagcag cctgcagcta gcttcctagt ttgctctttg attttcattg
    70561 agtattaatt atttttaagg ctaaagcttg gctggatatg agggaacgct ctgctacagg
    70621 tgctcaaggt ttaagtaagt ccattaaaaa gcatttgaat gacattaccc gtttgacagc
    70681 ttccttgcta ggagatgaaa agttatcggc tataacatca agtagtgtaa aagcagacat
    70741 acaccgcttt gtgatagaat tagagcctgt gaagtcaact attcttcaaa atgatgacat
    70801 ttcattggat caaaatgaaa tttttgaaat tctgaaaaat tttctcgatg gttaaaataa
    70861 ttgtagcgag atggctatat tgaattcgtc tatatctgga aactagaaaa aacttcaatt
    70921 tcaggagaaa atgaagtcaa tcttcccaca atcaaacgta tagtatcaag gtttttcaag
    70981 acctgatatt atgcgttttt tgcttttcaa aactttttgc ctagtctttg tttttatcct
    71041 ctagtcactt gatttgtttc aggtggtttt ttagtatagt gaaatgaaac gagaacagga
    71101 caaattgatc aggacagtca aatcgatttc taacaatgtt ttagaagcag agatgtacta
    71161 ttctagtttc aatctactat agttaaatct gcggtcaagt ctactggtga atctatgatt
    71221 gtaatactct tccaaaatct catcaaccac gtcagtctcg ccttgcagtc tgtatcttac
    71281 tgaccaagct agtgatggac ttagaatagg tgatttggag cgtcctattg gctaggaaat
    71341 gctgatcata gtcctttgct gaggctaggg tgtttcaaca ttcaacactc aactggttga
    71401 tctagttgat aggaagggaa ttactataaa atactcaggc ttccatcata ttttttgaaa
    71461 cgattgtgta atcaaaatgt accaatattg tagtattagt acagaagatg ttgtgaatgg
    71521 ataaatatat cataactgct gtctcaaaaa gatttcatat gtctgtgcat atataataga
    71581 cttcctgcga aaccaaaatc ctagttcatg attgataata ccagcaatca aattcattcg
    71641 taatccgaag cgtttacgat gatttcgata ggttgttgaa aacattttaa acatttctac
    71701 tttggcaaag atgttctcaa ccttgcttct ctccttagat agcgcatggt tataggcttt
    71761 atcttcaact gttagcggct tgagtttgct ggatttacgt ggagtttgtg cttgaggata
    71821 tatcttcatg agcccttgat aaccactgtc agccaagatt ttaccagctt gtccgacttt
    71881 tttgataagc tgtttttaat acagctaaca tctcttcaaa agtggtacgc tgaacactaa
    71941 caagacgctt aaatcgtgca tcagttagtt gtttacttgc ttcataattc atagaactac
    72001 tataccatat tttgtttcgc aggaagtcta ataagttata gtccagtttt tactatactt
    72061 tgatattcaa atatgttata ctatggttat caaagtaaat gaaaggagtt acgaatatga
    72121 cgccagaaga aatgtacctg acagagcgat tagacgtaca gatagctcat tttttaaaga
    72181 aaagcgttca acatcgtaga cgctataagg tattaaaaat aacagaaatc gtggcaggtt
    72241 tcctcatagc tgtcttttgt gctattccta tgccaggtga tcgctaccgt ttgatttcgg
    72301 ttgccttatc cagtctcggc ttgctgtgtg aggggattat caatttgtat aatgcaaagg
    72361 aaaattggat ttcttaccaa aaaactgcgc aactcctgga gaaagaaaaa ttcctctatc
    72421 aatgccaaac ggagaaatat gcaggaaaga ccaaggcttt tgccctattt gtcaagacat
    72481 gcgaaggtct tatctcagag gagattaacc agtgggaaag tatccagtca aaagaagtgg
    72541 cagctagtgc agatgctcca gttaaaaaag agtaggaggt agaggaaatg tctcaatcca
    72601 gctacctgtc gcccttgctc tggttaaaaa aagaagccga taaggaaaag atgagcgcga
    72661 cccagtgcca gatatttttc ttttactatc aaatgtttga gctcttattt gctagagaaa
    72721 gcgacatgaa agacttatgt ctgggaacga aaggttttta tttctcgcag ttagagaaaa
    72781 atttgctttc tggagtttcc cgatttctaa aaaacttgga ggggaaagta actctcaagg
    72841 ctaaccaaga agtatcagct cgcaaagccc tttttctagc cttgacaact agccaatcag
    72901 attggcagga gttagctcct gtttttgatt tttatcagac tatcgggagg cttgaaaatc
    72961 cttctctctt gagttctcag gacagacaac atctgatgtg gatttaccag tcagctttgg
    73021 agaaggatta tattgtcaag gttattggcg acaagcattt tgtattgaag agacaagatg
    73081 ctactaaatt gacagcgcgc caaactcaaa ctttggaaat tctgagtcaa tcagaagact
    73141 tggtcaatcc tgtctatgtt acattaggag aaaagggggt gctcttgctt gattaagaga
    73201 ggagatgttg tagttcttta tttgcctttt ccgactatta gtagcgattt ggctgtgaag
    73261 aatcatatgt atatctgtat tgacaacagc atgactaaaa acaaagagtt ggttaaaaat
    73321 cagaccttca aaccagctct cttgaccaga cgtttggtca agaactttat gatagaagag
    73381 ccggatctag ctcgtaatcc ttttacaaga ccaaccttga ttgacttaga taaggtattt
    73441 atgttggata atacggttat tccgacttct tatctagcca gacggcgacg caatgtctca
    73501 gaagaattgt acgaggaaat tttggatcac ttagtccaac cacggctgat ttcgctgaac
    73561 aagtctgagt ttatgcaact caatccagga acttattagg aggtagaaga tggcaaataa
    73621 agcagtaaat gactttatac tagctatgaa ttacgataaa aagaaactct tgacccatca
    73681 gggagaaagt attgaaaatc gtttcatcaa agagggtaat cagctacccg atgagtttgt
    73741 tgttatcgaa agaaagaagc ggagcttgtc gacaaataca agtgatattt ctgtaacagc
    73801 taccaacgac agtcgcctct atcctggagc atttctcgta gtggatgaga ccttgttaga
    73861 gaataatccc actcttcttg cggtcgatcg tgctccgatg acttatagta ttgatttgcc
    73921 tggtttggca agtagcgata gctttctcca agtggaagac cccagcaatt caagtgttcg
    73981 cggagcggta aacgatttgt tggctaagtg gcatcaagat tatggtcagg tcaataatgt
    74041 cccagctaga atgcagtatg aaaaaatcac ggctcacagc atggaacaac tcaaggtcaa
    74101 gtttggttct gactttgaaa agacagggaa ttctcttgat attgatttta actctgtcca
    74161 ttcaggcgaa aagcagattc agattgttaa ttttaagcag atttattata cagtcagcgt
    74221 agacgctgtt aaaaatccag gagatgtgtt tcaagatact gtaacggtag aggatttaaa
    74281 acagagagga atttctgcag agcgtccttt ggtctatatt tcgagtgttg cttatgggcg
    74341 ccaagtctat ctcaagttgg aaaccacgag taagagtgat gaagtagagg ctgcttttga
    74401 agctttgata aaaggagtca aggtagctcc tcagacagag tggaagcaga ttttggacaa
    74461 tacagaagtg aaggcggtta ttttaggggg cgacccaagt tcgggtgccc gagttgtaac
    74521 aggcaaggtg gatatggtag aggacttgat tcaagaaggc agtcgcttta cagcagatca
    74581 tccaggcttg ccgatttcct atacaacttc ttttttacgt gacaatgtag ttgcgacctt
    74641 tcaaaatagt acagactatg ttgagactaa ggttacagct tacagaaacg gagatttact
    74701 gctggatcat agtggtgcct atgttgccca atattatatt acttggaatg aattatccta
    74761 tgatcatcaa ggtaaggaag tcttgactcc taaggcttgg gacagaaatg ggcaggattt
    74821 aacggctcac tttaccacta gtattccttt aaaagggaat gttcgtaatc tctctgtcaa
    74881 aattagagag tgtaccgggc ttgcctggga atggtggcgt acggtttatg aaaaaaccga
    74941 tttgccacta gtgcgtaagc ggacgatttc tatttgggga acaactctct atcctcaggt
    75001 agaagataag gtagaaaatg actaggagag gagaatgctt gcgacaaaaa gaggcgatga
    75061 tctctctgcg gatatctgaa agtttatctc ttgcctagcg atttccattg gagtatgctc
    75121 gtgatgttaa aatgaatcta tcaaacagaa tgtgtaaaaa agtaaaaagg gtagccaatt
    75181 ccttgatata caggggattg attatccttt acttgtttta gatgattgga aattcaagaa
    75241 cgacacgtat cggaaatatt ttgctgttta tatctaatac ccatttatgg aaaaaatgaa
    75301 attaaatatg aacaaatcaa ttgctagcaa tggtttagaa gtgccagtgt actagtctaa
    75361 cttctccatc ttgggaacac gtaaattata ctcttcgaaa atctcttcaa accatgtcag
    75421 ccccatccgc aacctcaaaa cagtgttttg agcaacctgc ggctagcttt ctggtttgct
    75481 ctttgatttt tattgagtat tagaggtttg cttagaaact cttctattga tttcttagaa
    75541 tacaatagcg cctataagaa ctgttgaatt tcaaagtatt tacttgaact atgatatgtc
    75601 tggagtagac tgtcaacaaa aggtgcagta tgattgtttt tgtcgcttat atctacaaac
    75661 ttcaaacagt aatttgagct gactttgtca aacttggctg aaactttcta tttgcagata
    75721 atggtctagt ttgttttttg aatgtggatg agtgtctgtt gataaagata gaaaggtaag
    75781 accatcttag caggtaaagt gctatcctag cttgatttgc taataaaatc catttcctct
    75841 agtgaaaatc aaaaaaactt gtgttataat aataaagatt aaaatgtgaa aaaaggagat
    75901 tcctaatggg acgtaaatgg gccaatatcg tagccaagaa aacggctaaa gatggagcta
    75961 actctaaggt atatgcaaaa tttggtgtag aaatctatgt agcagctaaa aaaggtgatc
    76021 cagatccaga atcaaactca gctttgaaat tcgttatcga ccgtgctaaa caagcccaag
    76081 tgccaaaaca cattatcgat aaagcgattg ataaagccaa aggaaacaca gacgaaacct
    76141 ttacagaagg acgttacgaa ggttttgggc caaatggctc tatgctaatt gtggatactt
    76201 tgacttctaa cgtcaaccgt acagcagcca atgtccgtgc agcctttggt aaaaacggcg
    76261 gaaacatggg cgcttcaggt tctgtttctt acctctttga caacaaaggt gttattgtat
    76321 ttggaggtga agatgcggac gcagtctttg agcaattgct cgaagcggat gtggatgtgg
    76381 atgacgtaga agcacaagaa ggtacaatca cagtttacac agcgccaact gaccttcaca
    76441 aggctatcgt tgccctccgt gagtctggta ttgaagaatt ccaagtgact gaattggaaa
    76501 tgattcctca gtcagaagtg gaattgtcag gcgaagacct tgaaaccttt gaaaaacttt
    76561 acagcgttct tgaagacgac gaagacgtcc aaaagattta tacgaacgta gatgggtttt
    76621 aatttaacct atagaacatg atcctaagtg agaaaatcac ttgggatttt tttaatcaag
    76681 aaaatagttc tcatagaaat ttaattattc tccagagaac tatttttatt tagaaggggg
    76741 agattatgga taaaccgatg ttagtcttta agcgttttgg tcatcaaatt cacctgatgg
    76801 tgcaaaagga agccaaacgt tgcggcattg aatttatggg tgggccgcaa ggtcaggttg
    76861 tgcatttttt ggataatcgc gagaaaaacc aagacttggt cttgattaaa gatatcgagc
    76921 aggaactcaa tattaccaag tctgttgcta gtaatctggt taagcgtata gtgcaaaatg
    76981 gtttggtgga attggaggcg agtcctgttg ataagcgggc taagtttgtt cgtctgacgg
    77041 acaaagcacg ttctcagatg caacaggtta aggctttttt tgaacgcata gacaagcagt
    77101 tgatggaaga cattgatgaa gatgaattac tgatttttga gaaggttatc ggtcaactac
    77161 aggcaaaata tcaagggaat aggaggagag aataaagaaa ttagccaaaa gaattagtag
    77221 aaaagaatgg gggatgattt tactagccat tctctttact tgctttttgg tctatctaga
    77281 gttgaaagtg ccgacctata tctcgaaaat tacggatttg ctaggtagtc aagaaactaa
    77341 tttagatgag ttgtggcagc cggcaagcat gatgatggga atgtcctttc ttgccttctt
    77401 gtccgtagtt gcagttggat tttttgcatc ccgagtggcg gcttcttata ctagtaggct
    77461 gagaagtgat atttttaacc gagttttgga ttactcgcag acagagatta agaaattttc
    77521 aattcctagc ctcttgacgc gtactaccaa tgacattact caagttcaaa tgttgattac
    77581 tatgggcttg caagtggtaa cgtgtggtcc aattatggct atctgggcta ttgggaagat
    77641 tttaggtcat tcagaatact ggctctgggc cgtacttgtg gcagtgatta tcaacgtcct
    77701 gatgacgacc gttttgatga cgttagcctt tccaaaacag tccttgattc aggggctgac
    77761 agataaactg aacagtatca ctcgtgagag tttaacaggt attcgtgtcg ttcgtgccta
    77821 caatgcagag gattatcaaa atgaaaaatt tgcagcagta aatgatgaat tgacccgtta
    77881 aaatttgttt gtcaaccgtc ttatggctat tttgaatcct atcatgatgg ggatttcaag
    77941 tggtttgagt gtggcgattt actggattgg ggcctatgtg attaacgacg ctgctccgat
    78001 agcgcgtctg cctctcttta gtgacatgat tgttttcatg tcttatgcta tgtaggttgt
    78061 catgggcttc cttctcatgg gagcactctt catcgttctt ccccgaacta tggtctctgc
    78121 taagcggatt aatcaagttt tagatttgca ttcttctatc caaaaccctg ttcaagtaca
    78181 gctgactgat gaaaacttca aaggtcaggt cgagtttaag gatgtgacct tccgctatgc
    78241 ggcaaattcg gaggcagtta ttgaacatgt tagctttaaa gcagaaactg gtcaaacagt
    78301 ggcctttatt gggtcaacag gttctggtaa atcaactctg gtcaatctga ttccacgttt
    78361 ctacgacgtg tcagcaggag aaattctggt ggacggtgtc aatgttcaag actatgactt
    78421 ctctgcgaca gctcatgctg gtcaaaaggt tgccattgtt gggtcgactg ggactggtaa
    78481 gacaaccatt gtcaatcttt tgatgaaatt ctatgagatt gataagggaa gtattcacat
    78541 tgatggtgtg gataccaagg ctatgacgcg ttcagaagtg cacgatgcct tttcaatggt
    78601 cttgcaggat acctggctct ttgaaggaac tattcgagac aatctcatct ataatcaaat
    78661 agggattagt gatgaacgaa tgatggaagc tagtaaggct gtgggaattc accactttat
    78721 tatgaccttg ccagatggct atgataccat cttggatgac accgtgacct tgtctgtagg
    78781 acaaaaacaa ctattgacta ttgctcgtgc ccttcttaag gatgcaccgc ttttgatttt
    78841 ggatgaggcg acttcttctg ttgacacacg gacagaggaa ttgatccaaa aagccatgga
    78901 ccgtttgatg gaaggacgca catcctttgt cattgcccac cgcttgtcaa ccatccgaaa
    78961 tgcagacttg atcttggtca tgaaagatgg aaatatcatc gagcaaggca actatgagga
    79021 actgatggcg caaggtggct tctacgctga cttgtacaat agtcaattta cagaagatga
    79081 agcagaagaa taaatcaaaa gaaggcttga cggccttctt tttcttattt atactagtac
    79141 acatagaaag agagaagtat gagtcagaaa ttatacaata tgaaatttgc tgctgtctac
    79201 ttagccttga ttgcaaaagt tgagcgaaag ggtggaaagg cagagtcagt tcaccaagtg
    79261 accagttggt tgacagggta tgaagtgagt gatgttcttg cttgtctgga tagggatgtt
    79321 acttacggag atttttttag acaagcacca tattatgtcc ctgaacgaat tgcaattaca
    79381 gggaagattt gcagtgtccg tattgaggaa attgatgatc ctctgatgca ggaaatacgt
    79441 cgcttggaca agctagtgga ttggcttgcc aaggggaaaa catctcaaca agttctagaa
    79501 aagtatgaga agcataaatg aaaaattatc aagaatggta tcgaaatatc agctccagac
    79561 taactagtcg tccaattctt ttattcctat tacgcagttt caatcgtttg atgacagtcg
    79621 ccatgcccct ggtctatctg atcttgctag tcaccactta cctgcaacta ggacttggtc
    79681 agcaagttgg ggtttatgtg cttattcctg catcaggttt tgtggccttg tccctgtttc
    79741 gtaagaaaat caatcacccg agaccttatg aaacttggga tatctgtccc cttcttgata
    79801 aggatagttc gggacggtcg atgcccagtc gccatgtctt ttcggcaact atcatctcca
    79861 tggcctgtct gcatgctagt ctacctattg gcttagtatg cttgatcttt tcagctttgc
    79921 tggggttggt gagggtttta gggggtgttc attatcccaa ggatgtcttg gttggctatg
    79981 cttgtggttt ggtgtgggga ttccttttct tcctattctg acatgtaagt taagctagtc
    80041 ctacaaccac ttactgcttc aaattgacca cttgcttttt tgcccttgta ttcctaactt
    80101 agctttgata tagtagagtc agaaaggaga tgtgatgaag ttacgaattg agattgacgg
    80161 caatttagag gagactgaaa ttgtcatcaa gacccccact ttgacagatg aaattgcaga
    80221 cttgcaacgg ctcttgcaag agtcaaaggc tccgaggttg actttttaca aggggacagg
    80281 tgaatattat ctagacctgt cagaaattct cttctttgaa acagaaggga gcaagatcta
    80341 cgctcataac cagaaggaag cttatgaggt tcgcctcaag ctctatgagt tggagtctat
    80401 cttgcctcgc tattttaatc gagtttccaa gtcaacgatc gcaaacatcc gtcagattta
    80461 ctcagtggac aagtcctttt caggaacggg caccatttcc ttttatcaga cgcacaagga
    80521 ggttcatgtc tcacggcatt accaatccct cctaaaagaa aatctaagaa acatgaggta
    80581 aaaaacatga aaaagaaagc atttggtatt gttttgttgg ttttagcagc ttggatcttg
    80641 ctgcaaggga attttggaat tccttctttg gatggtaaaa tatggccttt actaggtatt
    80701 gttttttttg cttataagtc cattgagtcc atccttagac gtcatctcac ttcggcagtt
    80761 tttacaggtt tactggcgct catcattgca aattacgctt atgacttgtt accagttacc
    80821 aatcattctc ttatttgggc tagcatcttg gtggtacttg gtgttggtta tctgacgcat
    80881 tcaagtaagt tctggaatga aaaaaaatgg tggtacaatg ggaaaaaaac agtcgtcacg
    80941 gataaggaag tcgcttttgg tagcgggacc ttctataagc aagatcaaga tctcgtagat
    81001 gaccaagtgg aagtcgcttt tggggatgct aaaatctact atgataatgc agagatgcta
    81061 ggtgattttg caactttaaa tattgaagtg gccttcggaa atgcaaccgt ctatgttcca
    81121 caacactggc gtgtagattt gaaagtagaa acctcctttg gtgcagctaa ggctgacgct
    81181 cctgtagccc caaccagcaa aaccttgact atccgtggag atgtggcttt tgggaagttg
    81241 gaaattgtct acgttaaata aaaaaatctt cacttcaacc atcaaaatag acgtactaag
    81301 agtaggaaat tgatgccttg ctctgatttc agttctatgg ttgttagact ttaaaaaatg
    81361 aaatgctgcc tttaaaagtt gtatattttt cgatattttg gcttttacgt ttgatgtatc
    81421 tatgtactac agcgtagatg atgtagtgtc aaatgctttt aaaaaacgaa tgatattgga
    81481 cagttttttt gcctttaatt gctcaggaac catgaaagtc agtacttggg tttatgacaa
    81541 gggagaatgg tattatgtaa gttcttctgg ttccatgatt gcgaatgatt gggtcaaaga
    81601 caatggcaag tagtattatc tggcttcatc aggtaagatg cttcgcaata cctacacacc
    81661 tgatagttac tacgttggca acttaggtgc ctggcaataa gaataatact ctttgaaaat
    81721 ctcttcaaac cacgtcaacg tcgccttatc tacaacctca aaaccgtgtt ttgagcagcc
    81781 tgcggctagc ttcctagttt gctcttagta taagaagtct ttttccttaa aggaagaggc
    81841 tttttacttg cttttctgct gcttcctcat ttgtcctgaa gccttttttg tgttaaaatg
    81901 aatggtatga aagctaaaaa aatgtggatg gcaggcttgg ctctgctagg tatcggaagc
    81961 cttgctcttg ctacgaaaaa agttgcagat gaccgtaagc tcatgaagac tcaggaagag
    82021 ttgacagaga ttgtgcgaga ccttttttcc gacatggggg aaattgcgac cctctatgtt
    82081 caagtttacg aaagcagtct ggagagcttg gttggtggcg tcatttttga ggatggccgt
    82141 cattatacct ttatctatga aaatgaagac ctagtctatg aggaggaagt cttatgatac
    82201 agccagcaag tttagaagaa ttagcatctt tagtggaaaa agcgggcaag aaggtcttcc
    82261 tttttgtggc agactggtgt ggcgattgtc gttatattta tcctgcctta ccagagattg
    82321 aggagaccaa tccagagttc acctttattc gaatggaccg agatcagtat atggatttgg
    82381 ccaaactctg ggatgtttac ggaattccta gccttgttgt tctagaaaag gacaaggaaa
    82441 tcggtcgttt tgtcaatcgc gaccgtaaaa gcaagcaaca aatcaatgat tttttagcag
    82501 gattgaaata ggagaaaaag gaaacaatga tttttacgta taacaaagaa tatgtcggtg
    82561 atgtccttat ggtcatcgtg aaaaatagcg gagatgccaa actggatgtg gagcgcaaag
    82621 gcaaggtagc ccgtgttttt ctcaaagata atggggaaac agtggcttgg aatattttcg
    82681 aagtttcaag tttatttgaa attgcagagc gcggtcaagt ctttttgaca gatgagcaag
    82741 tcgctcgttt gaaccaagag ttgcaggcgg aaggttttac agaagaaatt gttaatgata
    82801 aggaacctaa gtttgttgtc ggtgaaattg ttgagatggt agcccatcca gatagtgacc
    82861 acctcaacat ctgccaagtt gcagtcgcaa gtgataagac tgtacaaatc gttgcaggag
    82921 ctcccaatgc gcgtgtgggg ttgaaaacca ttgtcgcgct tcctggagct atgatgccaa
    82981 aagggaatct cattttccca ggtgaacttc gtggtgaaaa gagttttggt atgatgtgca
    83041 gtcctcgtga attgcatctg ccaaatgctc cgcaaaaacg tggggtgctt gaattatcag
    83101 aagaccaagt tgtcggaaca ccgttcgacc cagcgaaaca ctggactgcc taggaacttg
    83161 tcagcatctg atagaaggaa ataagatggc aaaaaatgta gtgattacgg gagcaacctc
    83221 aggaatcggt gaagcgattg cgcgtgctta tctggagcag ggtgaggatg tcgttctaac
    83281 aggacgacgg atagacagat tagaaatcct caagtcggag tttgcagtaa gctttccaaa
    83341 tcaaactgtc tggacttttc cactagatgt gacggatatg gtcatggtga agactgtttg
    83401 ctctgatatt ctagaaacga tagggaggat tgatatcttg gtcaacaacg ccggactggc
    83461 tcttgacttg gctccctatc aagactatga ggagttggat atgttgacca tgttggatac
    83521 caatgttaaa ggtctgatgg cggttactcg ctgtttcttg ccagcaatga taaaagtcaa
    83581 tcaaggtcac gttatcaata tggggtcaac cgcaggaatc tacgcctatg ctggtgccgc
    83641 tgtttactca gctaccaagg cagcggttaa gaccttttcg gatggactgc gaattgatac
    83701 catcgcaacg gatatcaagg tgacaaccat tcagcctggg attgtcgaaa cagatttctc
    83761 aactgttcgt tttcatggtg ataaagagcg ggctgcgtcc gtttaccaag gaatagaagc
    83821 cttgcaagct caggatattg cagacacagt agtctatgtg accagtcagc ctcgccgtgt
    83881 tcagattaca gatatgacca ttatggccaa tcaacaggcg acaggtttca tgattcataa
    83941 aaaataagaa atttcctcga aaagttacaa atttctgtaa ctttttttga tttcctacga
    84001 atagataagt aggaggaaga aaatatgtat aataaagtta tcttaattac tttgactaaa
    84061 atacagaaat acgctagacc ctttaaaaat ctagcgttat cgacatgccg tcttttgtaa
    84121 caataatttc ttttatgata tttttgacaa tttttgaagc atcttcgtag cttaattttt
    84181 caacatcgaa gtcctttagt agccgtgtta gctttctctt tcggatgctc agaacattcg
    84241 atttttgatt ttcgagctgt tcttccagga attgcctttc tgttttgatt ttatcatttt
    84301 tctcgtccaa ctctttccga gtgatgattt cgtccaagta caattctgtt aattttgaga
    84361 cttgattatt tatccgttcg agttgtttct ttatctcctc gaccttgata gtctcttctt
    84421 tcttagcgag tgtctcattt ttatattttg gatcaaattt aattctttca agttccttga
    84481 ttacaaccct ttcaagttcc tctttagagt accaccctga ttcgcatctt ttttctaaat
    84541 ctttgctgtg tcggttcctg cattggtatt tatgatgaga gattccattc ttgtcttttc
    84601 ttgtatatct cagaccaagt gaagcaccac aatatccgca tttcattaat ccagaaagca
    84661 tatactttgc cctgaacggc cttggattgt tatatttttc ataggctgaa atctgccttc
    84721 tttcgacttc aagctggact aagtcaaaca attcttgagt gataattggt tcatgctgcc
    84781 cctcgtatac cttatctttg tactttactt ttcccaaata ggtttcattt ttcagcaagt
    84841 atttcgtgat tgtttcgccc caaggtcttt ttcttccaac gtgaccttca gcatttaagt
    84901 ctctgatgat cttgactact ggcttaccgt tcaagtattc cgtgaaaata cggttgacaa
    84961 tgagcgcttg ggtcggattc actgataaga cgccagtttc ttttgagtaa tcatagccga
    85021 atggtattgt cgtccatgac atggattttc cattctttgc ccgtccttct tttcctaaca
    85081 tcatacgctc tttaatctgc tcacgctcca gctgagcgaa tactgatagc ataccgattg
    85141 aagccttgcc gaaaggcgta gaagtgtcaa agttttcttg caagctgata aatgccacat
    85201 cgtttttagc aaacacatct tcaatcagaa acagagtatc tttctggcta cgactgaggc
    85261 ggtctagctt gtagactagc actatatcga ctttcttgcg cttcacgtct gagattaaac
    85321 gctccaattc aggtctttga gtgttagcac ctgagaaacc accatcaacg tagacatcgt
    85381 agattttcca gtctttgatt ttacagtagg cttcaagctt gtctttctgc tcatcgatag
    85441 agtaaccctc ttccacctgc gaagtagtgg acaccctgac gtagattgcg actttattcg
    85501 ttgttttcat tgaatttgta cccctttttt gataaaatag gtacaagaaa acgtctcaaa
    85561 aggtaatatc ttttgaaaag tttttcttgc cactagcctc acgctcggag tcgccaaact
    85621 ttgagagcgt gaggcttttt ttgtttgcaa ttatttccat tttggaaaca gttggtttta
    85681 ttctttcgat aagtgttgtt gaagaattaa ggccacgttg gctttctctt cctctgtcat
    85741 aggaggttcg tttggatcgt ctaccgaaaa ctcgatagca tgccatttat catttactct
    85801 aatccactct cttcgtctgt ggcatttgca atctaggttg tgtttaatca cttccatcgg
    85861 tctactttcg ttactcatgt tatttctccc tatacaaatc cacgacttcg ccgataattc
    85921 ggaagtcggt gtctggtgta attggcatat ctttatattc agggtttagg ctatgtaggt
    85981 aagcctggtc ttcatcaata acaagctgct tgatataagc atcgccgttg tagttgaata
    86041 ctccgataac tccgtcattc aaatctacac ttgtctgaat gaataccagg tcaccatcgt
    86101 ggtagtcagg ctccatggag tcacctttga tggggatgac aaagtcagca tcaacatcta
    86161 ctggtaactc aatccgttcc actcgtacat cattcaaata ctggcctgta cctgcggaag
    86221 ctgggtggtc gtagtagtcg taactgtaga gccgaatagc ttctgatact tcgtttatct
    86281 tcgtttcttc ttcgtttttt tgctctttca gttgcctctt tgcataattc aggactttgc
    86341 cctgtctagg tggttctagt tcgtcgtaga tggtttgaat ttggggagta ttagaagaag
    86401 agtcattgac catgaaatta ttagttataa atctaggatc taatacagat ttaggaactc
    86461 caaaaaaatc tgcaattttt tgaacattac cagggattgg caaagaagtt ccttttacat
    86521 accctgtcaa tgtactaggc ggtattcctg ttgctcgaga cagctcagct tgtttacaat
    86581 ttctatcaga taaaattgag ttaagatttg cagaaaagac tttcatatcc tctttatctt
    86641 gaggagttaa tttccctcgt cctcttgcca tatttttacc tcctatcttc tttactatat
    86701 aataccgttt attttcgatt ttgtaaataa aaaattcgaa aaaattacga aaaaattcga
    86761 aaaatcattg acatacgatt taaatcgtag tataatataa tcaagcttaa ggaaataaca
    86821 aaaacaaacc ggagggaaac accatgaaca cattaaacga gaaagcaatc aacatcttta
    86881 aagcagtagc taaggaaact ttaatccaag gcacttacga ggaaaacttc ctttacagcc
    86941 agcttgaagc attctgtact aactgccgtc aattcgcttt tggatggaca gagttagcag
    87001 aggagattga acgccaagag cgttaccttc tcgattctgg tttcactcaa gaggaaatcg
    87061 atgacattcg ttttgatgca gcatttgcag gaatgcttga taaattaaat gtagcctgat
    87121 tggtatcacc aaggttcgaa tccttggcag gttgttgctc atagagcgaa aaaagagaga
    87181 aaggagtaga acgatgaatg aatcttttct tacgatacta ggcatatcaa tgattgctag
    87241 ttttatcacg aagccgacca gttgaaactg gacgactcag atgtagaaat catttctaaa
    87301 agactaggac ggtctatcta aagaaagggt gaaacaaatg gcaactaaca gaactatatc
    87361 agtaaataca tcagagcatg atgtattgtt gacggcaaga aaaaaccacc ctgctgtatt
    87421 cgtcgatgga atgtttctcg acggagttga gcgagtggaa tttaccaatc attatctgga
    87481 gaagtgtgaa gttgttttaa cgtttaacga tagagttgaa accaatccct tccctctaaa
    87541 cgatattact ttattagaaa agttatttgg tcagagttcg aacggtcaat ctttacggga
    87601 tattgtcgtg caaactcttg aagatgctga ttagcatcta aaccatcaaa aaaagaaaca
    87661 tgaacactaa aactttcttt tccgtttttc ttggttctat caaattcttt gccaaggaca
    87721 attagagaag cttctaattg ataatcagtc ataacatcac ctcctttcta gctttattat
    87781 agcagaattg cgaggaacaa atagaaaaat aaggaggtag gaacgtgccg aaaatgacat
    87841 tgagagcaat aagaacaaat tataacttat ctgcaaaaga agttgccgat aaacttaaca
    87901 ttcatcaaca aacactgttg aagtatgagc atgatagttc aaaaattcca atggatcttt
    87961 tagataaact tgctcgacta tacaatgttg aaaaggattt tattttttta ggcaaaaaat
    88021 acgaattaaa tcataatcta ggagaggtgt gaatgaacga gcgagagtta tagaaagggg
    88081 attaaatatg aggtatgcag tatataatca ggaatactca tgggaattac acatctttga
    88141 ataatgctta tgctcaagac aaacgtttaa aggcaacaac gataggtatc cttacagtaa
    88201 tcttgatgaa taagtctgat tgggttgtgt atcctgacga gattgcacga cgtctaggaa
    88261 taagcaggcg caccgtagat gagcacttta agcttttaga gaaagcaggc tatctcagag
    88321 tatatcgctt agggttaggc agaggtaaag gcgtaacggt atatagattt ttttcagata
    88381 tgcctatttc agatgattac tttgagtatc taaaaactaa tcttgagaaa gagttatcca
    88441 cagataacgg agtttaaaaa tacagttgga aaatattgcc atgtgtaaaa ttgccatgtg
    88501 taaaattgcc atgtgtaaaa ttgccatgtg taaaattgcc ctctaataag tactaactat
    88561 acaacaagta ctaactatac aataatctaa gcctaacggc actaacttag taataactac
    88621 taacttacaa caaactaata cttctctaaa aaataaaaga gagaaatttc aattttagga
    88681 ctttgaaaaa taggaaagga gaaaaaatga gaccaagaaa atatccgtat agtttcaaac
    88741 caaatctgat gaacatttta gatagtcgtt tctatacacg gctaattgtt gaaacagagg
    88801 atggagcgaa aaaatagcag aagtcacact agatgatgta acttctgcta cagggtatgt
    88861 tgtaaggcta agaccgaatt atgactagcc tttaggaggg aatgggtctt taccgtggct
    88921 gtcacggctt tgaattttcc catctttgcc atgaatgatt agttcggaac cttgatttcg
    88981 tgaaatctgt ctagcaatat ttttagcttc attctttgta gtagtatgaa cagttgctct
    89041 tgaattgcca gcacctttca cgttccaacc accatttttg gcagggacaa catgctgatt
    89101 tttgcccatg attaaatctc ctttctattg gaattttgac taaaacggtg agaggtccta
    89161 gtcaagagta ttatagcaat ttaggaggat attacatcag tcttgaggct gatataggag
    89221 gttgaatgga agataaaatt atcgaacttg ctgactactt catcagcgaa tctacaacgt
    89281 acagagaagc taaaatagcg tgtgagaagc tattgaaaca agtcagccat gagatagaac
    89341 tcagggcaat ggaaagtaag acagtctaga agacaacaaa aagcacctga cggcaatcag
    89401 gcgcatacta aaaaacttta caagaggatt ataacatgaa tatgaactta aatacaaatg
    89461 aaattttaac aactactgaa tatgatatgt ttcgcaaaat tagtaataga aaaataactg
    89521 aaaatcctaa attggaagag gagctacttt ctgagggaca acgtcagccg attttggtaa
    89581 atgaaaaaat ggaagttatc gacgggcaac atcgtcttta ttacttgaga aaacatagaa
    89641 aaccagtgcg ctacataatt gatccgaccg ctaatttcaa gacagtaatt tcgatgaata
    89701 catcagctgt caactgggca ttacaagatt atgtgtattc gttcgcttta gagggagatc
    89761 ctgaatttgt taaattagct aaatttttag acgagaacga attgcttagt gacaagatgg
    89821 taatcgtagc tggttcagga agacgtgatg gtacggcagc acacgtagtc aaaaaattaa
    89881 aaaaaggcga ttatgtattt tcaaacgaaa aacagttaag agaattttgt aagttctacg
    89941 aacgcgtttt aaacgaaaca aaactgccta acaaaccatt tttacaatct gttttatgga
    90001 ctttgtatac aacatcagtt tttgatgaaa acagaatgtt gacacaattg aagaaatcag
    90061 atttgacatc agaagatatc gaaggatttg caaagaaaaa attgctatta acttttttag
    90121 agctatacaa cggaagatgg agtgacgatc atccttcttt aattcaatac tttatcaaca
    90181 gaaaagggtc gttaacaatt cctagtttgc ctaaacaaga tgaagataat taaaaaagta
    90241 ggaagataaa atgaaagtca cagtatatgt ttacggccga aaattagaac cagatgaacc
    90301 aattatcatc ccagaaaatc atcgttttta tgacatttgg aacggaattg taaatgaaat
    90361 gctcgacaac gaggaggatg tagcttaatg aaactactta ctaaattaaa actcagactc
    90421 gaaggagttc ttaaatcagt caaccttgac tggagagagg tagcagtcga acttatgaac
    90481 gacctatttg aggagcgcaa gcgtcgcttt gctttcgagc aagaaaacta tgacttgaag
    90541 caacaattgg caatctacaa agaaaaagaa caaggagaac aatatgttta aagcactaaa
    90601 aacaatcaaa aaaatcaaac agcttcagaa agaaatgcac gacgcaagcg tagcattcct
    90661 acttatgcaa gacctcggtt tggttcctga tagcgaaaaa ggaagaacca tagctaaatc
    90721 gttttatgat gtaagccaca tgctcaagga cgtcctgggc ggcaagtcgg tagatgaagc
    90781 catgaaacgt ctaaatagcg aagtgaaaat tgaagatgtg gagcaggaag atgactagaa
    90841 ttgaacttga aaaccgtgta tggcttttgg ctaatcatga agaaaaaaac gaattgctgg
    90901 atcttgggct aacatccaag gctagatatg tgaagcgagt tctggaacta ggaaaggtgt
    90961 atgctcatgt ttgattacga cagagacata atgcaaccgc ctgaagaacg agaagaacgt
    91021 gacccagctg attggatttt cagcgctggt caatggatct atgtaggaga ttgttagcct
    91081 atgaatagag agcatttaga gcgtgaaaat tggaatttga agaagacgaa aggtagaaag
    91141 aaatgaaaaa acgattatat tacaaaaaat ggaaacaaga acttagagag gcaatgagag
    91201 aagaaattga tggcgattat ctaaccgaga aaatggttag aaaaatgagt attagcgata
    91261 tgtcacacta tttgaaccaa ttagcattag aagaagctgg atactgtggg acaatgttta
    91321 attactaaaa gaaaaaggag agaaaatgac taataatcaa ttagcaacac agacaaaacg
    91381 taacatcact actgacccaa gtttattgac cggggcagac atcaaaaagt attttgaccc
    91441 acaaaaccta ctgactgaaa aacaagtagg tcaggcgcta gccttgtgta aaggtcgcaa
    91501 tcttaaccca tttgctaacg aggtctacat tgtagcttac acaaaccgta atgggggcaa
    91561 agagtttagc ttgattgtct ctaaagaggc tttcttgaaa cgtgccgccc aatgtaaaga
    91621 ctatgagggc tttgaggctg gagtagtggt agtagacagt gagggtgtta tgcacgaacg
    91681 caaaggggca atcatgctcc cagaagatat actgataggc ggatgggcta gagtgcaccg
    91741 caaaaatttc aaggtacctg tggaaatttt tgtcagtcgt gaggaatatg acaagaaaca
    91801 aagtacatgg aacaccatgc cagctaccat gatcaggaaa gtggctctag taaatgctct
    91861 tagagaggct ttccctgagg acttggggaa catgtacaca gaggacgacg gtggagagac
    91921 atttgaccgt atcaaagacg tcacacctca agagagccgt gaggatgtcg tagcacgcaa
    91981 gatggctgaa attgagcaat tcaacaaaga gcaagaggca aatcatgcag atcccgaacc
    92041 tgctcaaact gaggaaacaa tccagggcga attgctagac ggtgaacttg aatattagga
    92101 ggacaacatg caagaattac aggttattga tgataagaaa atcaataaaa tctatgagat
    92161 gatcacaacg gatgaactta ctagagagtc ttttgaaaaa gacctcatag aggctactga
    92221 gaagtacaag gactatattc ctacagctgg aactctcaaa gacgacaagg caaagcgggc
    92281 tgaatttaac aagttaattg agtctaaaaa tcgtatccgt attgacacta aaaacttact
    92341 atcagagaca gctaacacat gggatagtta tgctaagtca attattgacc catttgcaac
    92401 cgtagttagt gaatttgaca aaggtatcaa ggaaatcgaa gaacatcaaa gacaactaaa
    92461 aatagatacg gttaagagtt acctagccaa caaatcagct gagtacatgc tggatcctcg
    92521 tctctttgat gaaaaggccc ttgagtatgt caaagctagc gattttatgg cagatggcgt
    92581 gacgcttaaa aaagccacta tgaagtcact tgatgacatg gtcacatttg aatttcagaa
    92641 acaacaagaa tttgaaaaag ccaagtcagc tatttcaggg ttatgtgctg agtatggcat
    92701 gactgactca ccttacatta gacagctgaa agacttgact attgctgaag tctttggaca
    92761 aatcaaagct gattatgagt ttgaaaagca aaaggaagaa atcagacagg ctcaagaacg
    92821 agcagagcga gaaagtcagg aacttttagc agcccaacaa accaaacagc aagaacaggc
    92881 tccaaaatca acggaaaccc caaattttga cccagaaacg ggcaaaatct tggacggtgg
    92941 acaaatcctc caaaatgagc ctaacgctct tagaggggct gaaaatgacc taaaacgata
    93001 tgcccaaaaa atgactttag aggtgtattt tgtagacaca gccgaaaaag actgtttcaa
    93061 ggctggtcta agtcaactcg gatttgattt taaaaagaac tatcaagtca ggggttatca
    93121 acgtatcgag ccattaactc aggctgaact aaatgaacaa tgtgggtggt aagtatgaca
    93181 gaaattgaaa aaatttcaga agaattggct gaatatgggg tgcctgatga gttaatagga
    93241 aaaatagagg acctattagc gactctgtat ggcgaaaaaa aggaaattgg agatagaaaa
    93301 atcttggata tttctccaga gtcaatgggg aggtaaccat ggacatcaga aaaatatctg
    93361 acagcgtagc catctactcg gacggcaaga aattgcaggt tatccacaac ctaggggatg
    93421 agtttatcct tgatttcaat gtgggagagg atagcgtctg gaacctcaat ggccaagtcg
    93481 tagaaattat tgacatgatt gagcctgtct ttaaagtttt cagcttttgc tcaaaatctg
    93541 gagagggtat gcaacgctta aaacatgcta tcgtccactt tgaaatattt gagcagtaca
    93601 tcagagataa tcaggaagac ctgatgatct ggtggcacaa tccaggaggg gaatatgatt
    93661 aaaaccgtat ttttttcatg tgattatcca catcatgagg cgattgacga ccaaataaat
    93721 agctggcttg ccgaaaatcc aggcattaag ttgattgaca tcaaatttca atcaaatgtg
    93781 tctgctgtcg ctgacagtgg agtcagtgct gaatattggc atacatccgc attgattatt
    93841 tacaaagttc cctcagagaa caatataagc agtattaatt caaatggttt aggtttcata
    93901 atcagctgtg agaaatgtgg tagcttatca ataatcaagg gaaaagatgt aggtcaaaat
    93961 gtatgttatg aatgcaaagg agagaaataa tgaatgattt tatcaaagag attgggatgg
    94021 ctatcctatg gatgttttta gggtatctct tgggagagcg tagcactaga gggggacaaa
    94081 tcagatgatc aataacgtca cactggttgg gaggcttgta gcgcctcctg atctacgaaa
    94141 aacgcctaat aatgtatcta gtttacaggg cacgcttgca gtcaatcgca atttcaaaaa
    94201 cgaaaatgga gagcgtgagg ctgattttat caattttcaa gcttggagag gtacagctga
    94261 catcattgct cagtattgca gcaagggctc acttattggc ctcacagggc gcttacaagt
    94321 taggtcttac gagaaagacg gtcagcgtcg atatgtgact gaagtaatcg ctgagagtgt
    94381 agctctgcta gagagtcgca acagtcagca cggacaaggc aacagtttcc aaaatgggaa
    94441 tagctcacct tttaccgatc ctaacccctt tgacctccca aatgacggtt tgccgtttta
    94501 ggaggtatat atgtcaaaaa ttaaaattct tgacgcttgc tgtggcagtc gtatgttttg
    94561 gtttgataaa aacgaaagtc acacaatttt tatggacatt aggcaagaaa catttgagat
    94621 acatgacaaa aaggtcaatg tagaccctga tattatcggt gattttcgtg acatgtcttt
    94681 tgaagacaac acatttaatc tagttgtgtt tgatccacca catctaaaat gggctggacc
    94741 taattcgata atgaaagctc agtatggaca actggacaaa gttacctggt cggaagattt
    94801 ggccaagggt tttgaagaat gtctgagagt tctaaaagtt ggcggcacac tagtctttaa
    94861 atggtctgac cgtcagataa atgtaaagaa attactagag gtgataccat tcaagccctt
    94921 atttggtcag caaagaggca ccacacactg gctaacgttt gtaaagtttg aggaggacaa
    94981 gaatggagtg gacggattgg gtggattgga aacctgaaac caaaacggac attaaaacca
    95041 aaattgaaaa tgacgggtac acttttccac attacgacaa gaaaaacaat ggcgtcaagt
    95101 atgtgatttc tacaatggac atcaaacaag actgtctaag acttggagta ccgtttgaag
    95161 atatgtaccc tttgcaaacg acactttttt aacaggagaa agaacatggc aagtaaaatc
    95221 aatgtgacag aacgtattgc tatcatcatt gagaaacaaa aaatagaggt cgttacgact
    95281 ctaaactatg atatgagcat tagctttgat aacaaagaca cggctcctac actagatgac
    95341 aatggtgacc tttttgaacc ggtctacaag tgcaaagtta aggcaattcc caaaaatgat
    95401 gtatttttca cctcattaat acgggtcaag agcaatatca agacgttaca agaggttaaa
    95461 aaattctttg agttcgtaaa cgaaaacaga gaaaatctct ttgagatggc aggatttaag
    95521 ggggctcttg aatgaaattg accctgaaca ttgagcctaa acctcaatca cggccaaggt
    95581 ttgcaagacg tgggagtttt accacaactt acgaagacaa ggatatgaaa acatggcgca
    95641 atcattgcca gctgctcatt gctaatcagt acatgggtca gcctatcctt gagggagctt
    95701 tgagggcacg gcttagattt tatatcaaac ctcctcagta catttctaaa gtcaagaaga
    95761 accaacagac cctcctggat gaaattatac cagtaggcaa aaagcctgac atagataact
    95821 acgaaaaagc gctatatgat agcatgtcag ggatcgtctt ccaggacgac ggtcagatag
    95881 cgctacatga tgtaggcaag ttctacagtc taaatccacg gatagaggtt gagattgagg
    95941 tcatgaaatc cctgagtatt tgaagaaatg aggagcagat ggctgactac gcattatatc
    96001 aaggtgatgt gtttgttacg cttggaacat tagcgcagat cagtagcgag acaggaatta
    96061 ctgaaaggat gttaaagtat tacacttaca catcacacca aagacgacac ccaaacggta
    96121 gggccgttat taaaattgag gaggaagata atgagaatta agacggaaag cggaggagtt
    96181 ggaagatgat ggaagagtta aagcaaaaag ttaatgaagt atacaactgg acggtagaag
    96241 acgggaagcc gcaacctccc aagcaagatt taccacaagc ggtgaaagac cgggcggact
    96301 atttttggga aatggcagaa gatggtatga cgtttatggg agcgatggaa tgcatcttcg
    96361 ctgatgaaaa gcctacagac tatgatttgg gagctactaa ggattggttg ccaaaatcta
    96421 aggagtttga tgattgggtt ggctattcgc caggaatgtc tcaggtagtt attgcagttt
    96481 atttgattta tagaggaaac taagatgaat aagcaggaat tgattaaaaa attagaggaa
    96541 cgaagaacaa taactgggaa ttttcaaggt tatgtagttt ggtggaagga tgtaaaagaa
    96601 atctttgaac aactagacga atccgaaata ggtcacgcag atgaagctcc acgctacgta
    96661 aagaacatac tagcacgatt gcgagaattg ccattgcatg atagagaggt ttggttaaag
    96721 gctatcatga gcgaatttga acaggatttt agccgtgcaa aatggcgaga gggctacgag
    96781 caaggtaaaa ttgagggtat ggttgaacgt gaaaaagtca tagttccgca gtgtgtggcg
    96841 gaatatatag aatttaaaaa gaaaaacaat tttcatgttt acggtgcaat gagagtaatt
    96901 gaagatcatt atgataagaa agttcctgat tggttttacg aaaataacat cgaaaaattc
    96961 tgtcttgctt ggcttgacgg ctacgaggtt gaaaaagaga agcggtattt tgttaagatt
    97021 aaagggaata ttaaagaaaa tatgttggtt tatggagaac ttttgaaaag gtatttcttt
    97081 acaaaaagct ttagtttaga cgatgttata tattcccaca cccgtaaaga actagaagac
    97141 gcaaagttcg gctgggtgtt tgattgtcca gggattgaga ttgaggaggt tatagaatga
    97201 aacgcttcat agcaatctgg attgtcttgt cagctacttt gaatatctgg caatgtgtcc
    97261 atattaaaaa tcttgaaaaa aagcgcccga ttgtcatcta taaagctgat aacaaaggcg
    97321 cagaaatcaa aggcagagtc gtccacaaag aaaagattgg cgaactctac acgatcacaa
    97381 tacaaaatta cggcacattc gtggtgtcac aagataacta cgaatttttg aaaattggag
    97441 atgaggtgag attatgatac cgaagtacag agcgtatgat agtggctcat taagtcgtat
    97501 gtatcaacca gacgaagtga tggtcggtga tggcaatatc tggattattg atgaggattc
    97561 agttgctggt gactggatag taaataatga cattcacctc atgcaatcaa caggcctctt
    97621 tgacagaaat ggcaaggaaa tctttgaggg ggatatagta aaacgatata gaagtccttt
    97681 ttttaaagca gaatgggaat atcggattga aattgtaatc aaagaaaaag ccagtctttt
    97741 gttaggtcga agatttgaca aggccctttg tgcaagatca ctcgactcgc cgtttactaa
    97801 aagtgatctg ttggaagtca tcggcaatat ctatgaaaat ccggaagttt tggaggttac
    97861 tgaatgaacc cagaaaaaat tgacaatgta aacaaaccaa gccactatca aggtcggttt
    97921 ggaatggagt ccattgatgc tttaagaaac ttcatgacag acgaacagtt gaaaggcttt
    97981 tatcttggaa atgccttaaa gtatttacta cgtcatcaaa agaaaaacgg tcttgaagac
    98041 ctgaagaaag cacgcaagaa ccttgattgg ttgattgagg aaatggagaa caagaatgag
    98101 acctaaaaaa tatccgtaca cagggagcaa aataaataaa gtgactacaa caggaatagg
    98161 agctcaagag cttgtagttt tccctaacgt agcctttaga aaagacttgc tcaagcacat
    98221 tttctcagtt gtcaaacaac atgacaacgc tacaatcatt tacttcagaa ttccaaaagt
    98281 attcggatac gaggaggaaa gagcaaaagt atatctaagc tacgaaaaga cgatgatgat
    98341 actcaatagc tactaaaata aaaaaagcca agacactctc tgtctcagca ataatctcaa
    98401 taatattatt atatcacaaa ggagacagag agtgaacaag gctaaagaac tattgaaaga
    98461 gttgcagaat ctggacatgg acattcaaag ccgtatagat gaaattaacg agcttgaggc
    98521 aggtttgctc tcaagcccca agtggattga cgtcaaagtc caaggtggtc aagctagaaa
    98581 agttgatgat gtctatactc agcttgtcgt gatgaaagag gccatagaac aggatactaa
    98641 agaggttatc aacagaaagc ttgagcttgg taggttgatt aacaagctga aaaatccaaa
    98701 gagcaggtct attctcaggg tgacttacat tactaagatg tatgttgatg atatttgtga
    98761 caaaatggaa atcagcagaa caactttcta cacttggcgg aatatggcta tctctgaact
    98821 gaatgaggtt ttggagagaa tggaactaaa ttgaacttta caaaaccgta cgggaaaaaa
    98881 tgatacttgt tagcacagtt ttgtaattct gataaaatgg tagtatcaag aattgaaaag
    98941 agaggtctca gaattggtag atggttacct gtaatgtcag ggggctgtaa tggccttgga
    99001 ggttcaaatt ctcccctctc ctttgagtgt ttgtgtccta gaatggggta ggcagtaggc
    99061 ttagcattca tatatcactc attaacttaa aaatggttgc ggaagcgact ggacctcgca
    99121 tgattgcgta gctaattata ttccggataa gttataagct agagggtttg attccctcag
    99181 aggttttaaa gactacaaaa aataaaaaag aagtcaaaat ttaatacgta cgcaaggttg
    99241 tagtcgcctt gcaagaaggt cgcacatcgt gtggcttttt ttgattattc gaaaggtggt
    99301 gatggaaaat tgtaaagaag aacaggaata aaagaagcag agaacaagtt gatgagtttg
    99361 gtttaaatag ccgtgaacgc aattttgcag atgagtacat cgctaatggc aataatgcaa
    99421 cacaggctta tttgaaaata tatccaacat ctggcgaagc taatgccgct aacaaaggta
    99481 gtgcatgggt tagaaaaggt gagattttaa actacatcaa aatcaaaact aaagaacgac
    99541 tagatgcatc ggggttgaaa gctaatgaca ttatagatcg tcttatcgat attgcctttg
    99601 gtcgtccaat tgttggatat agtaaacaga cagataaaat cacgggagaa gttataaagt
    99661 acattgaata cgaaggtacg gcgcctattg acgagcagat aaaggctctt gaattgttag
    99721 gtaagtatct caagttattc accgacaaag tagaagctga cgtttctgga acggtggtgt
    99781 ttgcaaatga gtcagacata ccagattaaa caaagtgata ttgtaatcga cctacctaag
    99841 acagtaggag ctgggtacgg acagttctgg cgctcaagaa gtctttatcg tgttgtaaaa
    99901 ggttcccgcg gttcgaagaa gtccaagaca accgctttga attatgttat ccgtcttttg
    99961 aagtatccct gggccaactt gcttgtcatt cgtagatatt cgaataccaa caaacaatcg
   100021 acatatacag attttaaatg ggcgtgtaat gtgttgggtg tgactcattt gtttaaattt
   100081 aacgagtctt tgcctgaaat aactgtaaaa gcgactggtc aaaaaatact gttccgtggt
   100141 ttggatgatg aactcaaaat tacatctatt acagtcgata tcggtagtct gtgttgggca
   100201 tggttcgagg aagcatatca aattgagact gaagacaagt tcagcacggt tgttgagtct
   100261 atccgtggta gcttagatgt acctgatttc tttaaacaaa tcacagtcac atttaacccg
   100321 tggaatgaga ggcattggct caagcgtgta ttcttcgatg aagagacgag acgggctgac
   100381 acattcgcta ctacaaccac ttataaatgc aatgagtggc ttgatggagt cgatatcaaa
   100441 cgctatgagg atttgtatca tacgaacccc agacgtgcta gaatcgtttg tgatggtgaa
   100501 tggggagttg ctgaaggttt aatctatgag aacgtgaccg tcaaggattt cgataaggat
   100561 gaattgctac gagattcagc taataagtta tgtatcggtc ttgactttgg ttttactcac
   100621 gatccaaccg ctttgtgttg ttcgttgata aatgatacga cgaaagagat ttatgtcttt
   100681 gatgaggcgt ataaagtcgg attgataacc aaagaagttg cgaagatgat aaaggacaaa
   100741 ggttatcatc gctcacaaat cattgctgat agcgcagagt cacggctgat tgaagagctc
   100801 aggtcagaac atggcatatc tagaataaaa gagagtcgga aaggtaagga tagtattatg
   100861 gcaggcgtat ccaaattgca aggatacgct atttatgtgc atccagattg taaaaacatc
   100921 atggatgaat tttatagtta ctgctaccag cgagataaag aaggcaactg gttgaataaa
   100981 ccagaggata aaaacaacca cttgatggac gctttgcgtt acagccttca atgtatcgaa
   101041 ggtggaaaag caaccgtccg cagacgttct gattatggtc tatagagagg aaagacatgt
   101101 accaatattt aacctatcca cgggatggat atgatgaggg ttctttgaag aaagacctga
   101161 tttacaaatt gataacgata catagcactg aaggctcgca tttgaagaag cttaaaagct
   101221 actatttggg tgagcatgct atcttagaac acacgagacg caacgtgaac gcacctaatt
   101281 acaagacggt agccaatcat gccaaggata tcgcagacac ggctacgggc tattttatgg
   101341 gcaatcctat caagtataac aatactgctg acggtgatat cgatgaacta cttacagcct
   101401 ttgatggtgc tgagattgac caagtagatg ctcagaatgc tttgaacatg gctatctatg
   101461 gtcgtgctta cgagtacatc tatgctaaag agggtatggc tgagttggat tcaactagta
   101521 ttgatccgga gaatactttc atggtctacg atgatagtat tgagcggaag cctttgtttg
   101581 cggtctatta ctatgaagta aaagacgata cgaaagacac taccaagcac caggctgagg
   101641 tctttaccga aaatctgcac tatcacatgg tgctgagaag tacagattca ggaacaactc
   101701 agagcgagga ggcaacacct cacaaccttg gtcaaatccc aattatcgag tatcgcaaca
   101761 atcactttgc aattggtgac tatgagcaac aaattagctt gatagacgct tataattcct
   101821 tgatggggaa tcgtgtcaat gataaggaac aggctgtaga gtctatactt gtcttgtatg
   101881 gcacgcagtt agcagacact ccagaagacg ctaaggtagc aatgaagatt ctttctgaag
   101941 aaggtctttt ggaattgccg ggcgatagtg caagggctga gttcttgaag aatacgctgg
   102001 acgaaagtgc tactgaaatc ttgcgtacag ctcttaaaga ggacatctac acatttagcc
   102061 atgtgcctaa tttgactgat gagaatttcg cagggaatac atcaggcgta gccatggaat
   102121 ttaagctgat gggccttgag atgattacta agaccaagga agcgaactat aagcgaggat
   102181 tgcgtcagcg tattgcgatt tttgctcatt acttaggcat gaagcagatt gctttagagt
   102241 ctcattcaat cgttccacaa ttcagtcgtg gtttgcctaa gaacttgtta gaaatctctc
   102301 agattgtgaa caatttggaa ggcaaagtga ccaatagaca gcttatttct ctcttgccgt
   102361 ttgtggaaga ccctgacgct gagctggaag ccttggaaga agagaaaaag aagaacatgg
   102421 aagacatgcc gatgttcaac aaagacaaca cgaaacccga agacgaggta gaggatgaag
   102481 aatcaggagt attgggcgaa gaggaaagcc aatctgattt accagcagat ggacaaggcc
   102541 gaaaagcagg cagaccagtt cgataaggtc tatcaggaag ccaagactta cttggataag
   102601 gaagtcaata agatttttga taagttccaa cgtgattatg gtctaagtca ggtagaagct
   102661 agacaagtct tgaagaacat gaaagacaag aaaaatctga atgaacttcg taaagtactt
   102721 gaagcgagac cgaatgaccc gaacatccaa agattactag ctgacttaga cagcccagct
   102781 tattctttcc gtatgaagcg cctagagcgt ttgagcgacg atttagaccg tatgcgtgaa
   102841 tctatctatc attcagagaa gacaggctca gacgcctttt atagcgactt gatgaaggat
   102901 agttactaca aggctacctt tgacctgcag cagcagacag gactagcata cggcttttct
   102961 gggcttcctg agagcgagat taaacatcta cagtctttca gttgggtagg tgacggaagt
   103021 acctactcta cagacatctg gaagaatacg gggaagctta cttctagcat aaaagatgaa
   103081 ctacttatga gcctcatgac aggccgagat acacgagaaa ctgcacaagc aattgctgag
   103141 aggttcaatg taggtcagaa cgatgcaaga cgtttggttc ggacagaatc agcctttttt
   103201 cataaccaaa tggaactact cagctatgaa gaagcagaca tagaaaagta tatctttgcg
   103261 gccgtcttag acaagcgtac atcacggatt tgtcaggagc atgacaatca ggtctatgat
   103321 agggacaagg ctgtccctgg cgtcaattgt ccgcctatgc acccttggtg taggtctact
   103381 actgtcggat acgatgagga cgcagattac agcaagttga agcgcagagc aaggaatcca
   103441 gagacaggga agaccgagct agtacctgct gatatgactt ataaagagtg gtatagcaag
   103501 tatgttgcga aagacgggga aaaggtgtat aatcaagata caagagaagc caaggcgaaa
   103561 ttttatagcg aacaactatt gtccaaaatt tcaggagttg agccaaaaat tacaagtgat
   103621 atgcaacgta tcgcaggaga aaacaaattg gcaggtcttg aatttaggaa gaaaacagtt
   103681 gagtcattat cacgtaaaat tattgcagat agcctagttg aaaatataag tttgtcaaaa
   103741 gccgtgagta agattaatga cgccttaagg tacacaacta ttttcgattc cgatactttt
   103801 acagaagagt atttgaagat gaaacagaag cttatcgcag aaggttataa aattgtaaaa
   103861 gtaaaaaaca cttggctagt agatggacca tacaaaggtg tgaatacagt cgttgaaaaa
   103921 gatggtatca actttgaaat gcagtatcat actcaggaaa gtttcgactt aaaaaatggt
   103981 tcattacatg aactctatga gaagtatcgt gatacgaata catctgatct agaacgcatg
   104041 aaattattta aggaaatgct tgatttaagc aatgggcttg agattcctaa aaatatagag
   104101 agggtgaagt gatatgaaag atattaaata ctaccgcaca acgacgaaca atgctcaagt
   104161 acttcgtttg attgatggtg tcatgcaagt ttttgacatt gaaaaaaagt gggttaatag
   104221 catggattgg tttaataaaa tcttttttaa tgactttacg gattttgaag aaatttcaga
   104281 aaatgatgca tttacttata ttgacaggat ggtagcggca tgattgatat tgccttggct
   104341 atcgctaaaa aagcacatgc agggcaggta gataaagcgg gtgttgatta catacagcat
   104401 cctctctatg tggccagtca agtcaacact gaacaagaaa aagctgtcgc tcttttacat
   104461 gatgtgattg aggatagcga tataactgct gccgatttat tcgcgtctgg cttgtcaaat
   104521 gaagttgtta cagcggtaca aattttgaca aagaaaaaag gtcaaagtta tcaagaatat
   104581 cttgggaaag taaaatcaaa taatttagca agagttgtaa aacttgcaga tttgaaacat
   104641 aactcagatt tatcacgttt gaaatctgtt accaatacag actacgagcg tgttaaaaaa
   104701 tataaaaatg caatttatta cttaagcacc tagagaaatc taagtgcttt tcttatgttt
   104761 agaaaggagc gagaaatgaa atatcgtaaa aagccagtag tagttgaagc cgtgcagttt
   104821 ttagatacag aagaatctat agatgagcta tgcgattttg gattagatcc agtacggatt
   104881 gattacgcag acttaaaaaa tcctctttta aaaatcgaaa cgcttgaagg attgatggtt
   104941 gcaacagaag gtgactacat tatcaaaggc gtgcaaggag aatattatcc atgcaagccg
   105001 gatatttttg cagaaacata cgaaaaaacg gaggaataaa atgttagaaa aagcaaaaca
   105061 attggcatcg caagaatttt tacgcttatc aggtcgtgaa atcaaagcag aagactgctt
   105121 tgtagtttgg tttagcaaga ccctgcaaaa ctggaaagct cttgttagta cgaacgcaat
   105181 tacatcaagc gaaccttgtg gagattatgc agaaattacg cataacggag ataagaatga
   105241 gacttatgtg gatgtttacg ccaaggtttc aaatcgtgcc attaaagatt aggaggtgat
   105301 ccaacatctt gacttgcagg aatagactgc tataaattac tgtaaattgc tataaaccgt
   105361 gtcatatttg atgcggtttt cctatgccct aaccgtatgg aatcccgtac ggtttttata
   105421 ttgtccaaac tgtgccgatg acattaaaag ctgtactgtt ccgtcgccgg acgtaaagcg
   105481 agattatcga gtggcgacgt aatcgctgga ggacaattat gtcagaagaa atcaatgcaa
   105541 ctgtatctac tgaatcaact gagaccgtcg acactcaagg aaatgttgat acagtgcaag
   105601 aagaaaagca cgaacgaact ttcactcgtg ctgaaatcgg taagatgcta tctgccgagc
   105661 gctctaaatg ggaagctgag caagaagcca aggaaaatga agctaagaaa cttgccaaga
   105721 tgaacgctga cgagaaacag aaatatcagt tggatcagcg tgagcaagaa ctagctgacc
   105781 gtgaaaaggc tattgctcgt aaagaattga ccgcagaagc taaagcaatg ctaagtgaac
   105841 gtgacttacc tgttgagtta gtgaacgtgg ttgatttgac aagcgcagag acggtatcgc
   105901 agtctgtcgc tgtattgcag aaatcatggg agcaagccgt gcaaaaaggc gtacaagaaa
   105961 agctaaaagg cggagcccca atgaaacaag cgccagtcga tagtgacggt atcacaaaag
   106021 aagaatttgc tcgtatgggt tatcagagtc gaaatgaact ctatcaaaag aacccagaac
   106081 tctataagaa attgaaaggt taaaataaat gacagcagga caaactaaac tagccactat
   106141 ggttaaccca gaggtgatgg cggacatggt ttccgctaaa ctacctaaat tgattaaatt
   106201 tactccactt gcttatgtgg aaacagcact ccaaggacaa ccagggaata ctctaacagt
   106261 tccagcatgg gagtatgcag gagatgcgac tgaggttgga gaaggtcaag ctatttctcc
   106321 agaccaattg actactaaaa agaccactat gaccatcaaa aaggctgcta aaggttatga
   106381 aattaccgat gaagctcttt tgtcaggtct tggcgaccca ctaggtcaag ctacttatca
   106441 gcttggtttg gctattgcta acaagattga tgatgatttg gtcgcagtag ctaaaactgc
   106501 aacacaacat attacagaaa ctcctacaac tcttgcagca attgataaag ctcttgagat
   106561 ttttgaggac gaagaagatg cgcaatatgt tgctatcatc aaccctaaag atgctatcaa
   106621 gctaaaaact gacgtagcaa aagaatggac taaaggttca gagcttggtg cagatatggt
   106681 tgtatccgga acgttcggtg aagttgccgg tgtgcaaatc gtccgttcta aaaaagttga
   106741 tgaaggtaaa ggctttatcg tcaaagtctc tcctagccaa actcagacag acgatgccaa
   106801 caaatatggt gcgtttgtta tcatgctaaa acgtgatgtg gctatcgaaa cagaccgtga
   106861 catccttaaa aagacaacgg ttatcactgg cgatgaacac tatggtgttt acctatacga
   106921 ccctacacga gttgtaaaat tcggtgagta agaggtgacg atatgagctt attgctacga
   106981 cgtcattata tccaagagga gcaggttagc cagtattctg atttagagaa taagactcta
   107041 gaagagttga agaatctagc caaagaagct ggcatagctg gcgcctataa gttatcaaaa
   107101 gccgaaattg tagaggtgct ggaggattta aaaagtgaaa tttaaaatca aacaagattt
   107161 ctatgattgg gaatcaaatg tgaaacgact ggcaggaggg gaacttgaga ttactgagga
   107221 gcgctatgtt gagctggctg acaatattgc cagcaacggt gtcgctatct cagatgttct
   107281 tgagaaaatc ctccctgaac ctgagttctt agaagaggat tgatatgtct atagagttgc
   107341 tgaagaaatt aacaggcgaa gaagatactc agcttctcat gttgctccaa acaagggcta
   107401 caaatcttat cttgtcagag actaatcgca catctttgac acctgcttta agtcttttaa
   107461 tacctgaggt tgctatcgag ctccacaacc gctcaggagc ggaaggagag cgttctagaa
   107521 ccgaaggtgg tatagaagta gtctacggag aaaacggcct gtctacgggc cttttacagc
   107581 gtatacgtat gcatagacta gcaagggtgg caggccatgt ttttgaagca gagtagactg
   107641 aaaccttatc caatgcgacg gtttgaaaag actgtcactg aggaaggtgt cgcaaaagaa
   107701 ggatatgtca aggaagctga gacaatccgt cttgagttgt ggccagctag tagtaaacta
   107761 cagtctgaat tgtatggcga gcgtgtcaat gatattttga acgcaaatgc caacaagtca
   107821 gctactatca aagtgaaaga tggtgtgtgt atcgatagcc agacagaagt gactcacagg
   107881 gttatttcta aaaaggtcta cacacatcat caagttttgg agttagagcg tgtcagggct
   107941 actaggggca gataggctta tagctaaatg tagacgattg gctagtaaaa aaactggcga
   108001 ggatatcgtc ttacgtgcgg tacacaatgc tactataaag gttgtccaag cagatgcaag
   108061 aagactcgca ccagcgagag atggagagct tataactagt atcaaaacta gggcaaaaat
   108121 ggacggagat aaggctatag gcgaggttta caccaatcta aaatatgctc cttacgttga
   108181 gtttgggaca gggccaatag gacaagctag ccattcgggt atctctccag aggtcagcgt
   108241 cacttataag tctagtccgt ggtatgtgca tgaagaccaa atcaatgtag gaccttacca
   108301 ttttcaaaaa attggggagt tctacaagat gtatggtcaa cctgcccagc cttatcttta
   108361 tccagctttg agagacaatc aagagcgtgt gtctaagaat atttcgaatt atgtccgtag
   108421 aaagataaga gaacaaataa aatgattaat atcaagcctg ttatttataa agaattgcaa
   108481 aaggttgcag ataatgtgac cgatacttat cctagcgatt gggagaattt cccagtcgtt
   108541 atttttttag aagaacagaa caagccgggt gattggtttg atgaccagga acaaaaatca
   108601 tctatccgct ataaggtgga tatctttgat gataccagca ctagtgagtt agctgttaaa
   108661 atcaatcaga tttttgagtc tttaggtttg cgaagaaccg actgccaaga cgtgccagac
   108721 ccgtctcatt tgagacataa ggtcatgcgt tttgaaggtg tcgttgactt agactcagag
   108781 cttgtttttc aatttagaat ggagaattaa acatgttagc aaatggaatt acgctttctt
   108841 atagcaaaac aaaaggtagc tatactaagc ttgttgggtt gaaagaagta ccagagtttg
   108901 gtattgagcc tgaaaaagta gagaacacta ctcttgaaga taaagttaag aagtatgagt
   108961 tcggtattgg agacgcaggg gaattggaat ataaattctc ttacaaaaac gatagcgcaa
   109021 ctgctcctta ccgtgtattg cgtaaggcag cagacgacaa ggaaaaactc tacttcgaac
   109081 aagcttatcc agacggtact aaggtcacat ttgaaggtca agtgtccgtt aaattgggcg
   109141 gtggcggtgt caatgccgtt atcgatttca cacttaagat tgccttgcag tcagagttgg
   109201 aatttacaga cggtgttgga ggttaattaa atggcgttaa aatacacaac ttggaaagtt
   109261 actgacgaaa aagagttgaa gctacgtttg acatctcatc aagctgcaac tgtggaagaa
   109321 aaaatcggca tgaacttgtt aaagattttc atgcctgaag ctggcgaaga gttcacttta
   109381 ccgcctttga aagttatgct gttgttagtt catggagcct tgcagcagta tgaacatggg
   109441 tattcctttg aagatgtcta cgacctatac gatgaatacg tggacaatgg gggagaccaa
   109501 acaactttca tgacagaggt tttaatgcca ctatttgaag tatcgggttt tactccacga
   109561 ggaagcaaga acaagaaaac ttccaagaag aaaatgacag tagtcgagta atcttaacag
   109621 taacgcagat tattgagagg ctttatccta tgtttttgga cattggaggc aagcctcttg
   109681 atttttggga tttgacggtg cttgaaatca gagaaatgat tgaaagctac aaccgtgtca
   109741 aaacccaaga gcgtaaagag aagattattg actcatacag actttcgcag atgatttcca
   109801 atcacgtttc tttattactg tccaatgacg ctaagattgt tgagttctgg gagtatgcgc
   109861 ctgagttatt tgtagaagaa caacaagcgg tagaactgga acgacagaaa caagcacttt
   109921 tgttgcataa ggaacggatg cgtgaatttg cagagagaca taatcgaaaa aggaaggagg
   109981 aagtaaatgg caactcttga tgaattgaaa gtcatgattg acgctgagat agcgcctttc
   110041 aggaagaaga tgaaagaagt cgagaatcag gtcaaaggaa catctgacca agtgaagaat
   110101 gccactgcca aagttcgtga acagtcgaac tctatcggta gtgcgtttgg taagctggct
   110161 aagttcgctg gttttgcaat ccttggtaag aaattacttg atgttgggat gtattcaacg
   110221 cagacggctc ttgaagtatc agcgtctatg aaccaaatca agcgacagat gggcgagagt
   110281 tcgcaatctt tcttaaaatg ggttaacgat aacgccaacg ctatgaatat gggtgtgggt
   110341 gaggctacta actacggtgc agtctactca aacctatttt ctggatttat taaggacacc
   110401 aacaaattga gcgcctatac tgctaaaatg ttgcagacgt cggcagttgt cgctgaaggc
   110461 tcagggcgca cgattacaga cgttatggag cggattcgct caggtttgct agggaacacc
   110521 gaagcgattg aggatctagg aatcaacgtc aatgtggcta tgattgagtc cactgaagcc
   110581 tttaagaagt tcgcaaacgg gcaaagttgg caacagttgg attaccaaac ccagcaacaa
   110641 atccgcctta tggctattct ggaacaggct acagccaagt atgggaatac cttgtctaat
   110701 tctgtaaatg gtcgtatcag cctgtttaag tcgctgatga aggacgcagc attgaacctt
   110761 ggtaactcta tgttaccgat tatcaatgcc attatgcctg tcttgaactc ttttgctatg
   110821 gttttgaaga acgttactgc taaactcgct gagtttatcg ctttgatgtt caacaagaaa
   110881 gcaacagtga aagatggtgt tggtggagca gttggagaca tgggtaacgc catgaaggat
   110941 gttgcaggcg gagcaggaga ccttgctgac gcagtagacg acgctggaga ttcagcagga
   111001 ggacttgctg acaatcttgg agactccgcc aaaaacgcta agaaggccgc taaagagttg
   111061 ctaggtcttt tgggatttga tgagattaac atcttgcaaa aaccaaaaga tgacgacgca
   111121 ggcggttctg gaggcggtgg caaaggtggt aaaggaaagg gaggcggtgg cggacctttc
   111181 aaagacatct tgccagaagt cgagttgacc gacatggaca acaaattcaa gagcattttt
   111241 gatggtcttg gagataagct caaagggttg tttgacctct tcaagaaagg ttttgatgca
   111301 gcatttagac cagaaggtat aaaacgcatt aagactgcct tagaccaaat agctaagaca
   111361 atgggagaaa tcgccactga cccaagggtt gtgaatgcct ttaaccgaat ggctgagaaa
   111421 attgcttatg ctttagggca agtgacaggc tcaataacca ctatcgggct aggtatcggt
   111481 gttttccttg ccgaaagtat tgcaaatggc cttggaaggc aaaaagaacg cattaccagg
   111541 gcgctagtcg ctttgtttga taatgttggt aacctttccg aggcagtagg aaacatagct
   111601 caggactttt ctagtgcttt ctacgacgtc attacctcaa ctggtgcggt tcgtatcggt
   111661 agcgctattg tgtcaactct gttgagtttg acatctacca ttgttgaagt tggtagtaaa
   111721 ttagcaggaa gtttgtttaa aggttttgaa aaattcgttg tgacaagcgc tcctaaaatt
   111781 tcatcagtct tccaaagttt attagatact gttgcgcctg tatttgagag cattgaaagg
   111841 tctgttaaca aatttggcga tggcttaagt cgtgtttatg atgaacatgt agtccctact
   111901 attaactcta ttgctaatgc ttttaatggg ctaattgaca ttattcagat tctctgggag
   111961 aattcctggc aaccttttgc tgagttttta tcaggagtat tcggtgttag tattgaagga
   112021 atttcagatt tattaggagg tggcctttta gccactttgg gactattggc ggatgctatt
   112081 aagttagtgg cagatggttt caccgttttt tctgactggt gtaaagaaaa caaagaacct
   112141 atcgtagctt tgataacaac ttggcaaacg attaatttct tatcatgggc agaacaagct
   112201 ggaggacttg caggagcatt cagcttgtta ggtagtaagg tctcttcgat tgttggagga
   112261 attaagaatc taggtcttgc tattaaagca ttgacatttg ataagttggt cagttttggt
   112321 gaaacaatct atttgaacac cttatatgca aaagattttg tggtcaattc aggtaaaaca
   112381 attgcacagc taggaaaaac tgctttagaa cttggtaaat cagctctagc atggactgct
   112441 catgcagcga aaatgggatt agcaaccgcg gcgaaatttg cacattctgt tgcaacagga
   112501 gtcgctacag ctgcaacatg ggcttttaat gcagcgttag cagttttgac aagtccaata
   112561 acatggatta ttgcagcaat cgcagcctta attgctatcg gtgttttgct ctatcaaaac
   112621 tgggacactg ttgttgagtt tgctaaaact gcatggcaag gactatgtga ttttatcagt
   112681 ggtatttgtc aagcgattgg cgaatttttc agcggtctat ggacgaaact acaagaaatc
   112741 tttgagccga taggtcaatg gtttggcgag aaattccagc aagcatggga cgccattgta
   112801 aacatattct ctggcatcgg agagtggttc tctggtgtat tccaaggtgc atgggacgct
   112861 atcgttaata tcttcacacc aatcggctca tggttcggac aacgttgggc agatgtgact
   112921 agtgcgttgg ctaatatcgg ggcatggttt actgacatgt tccaaaaagc atggactggc
   112981 ttaacaaaca tctttagcaa actaggttca tggtttggcg agagatgggc agatgtgact
   113041 aatgcgttat ccagtgtttc aaactggttt ggtgagatgt tcactaatgc ttacaacgca
   113101 gtaaaagatg cttttagttc tattggcgac ttctttaaag gcgtttggga tactgttaaa
   113161 agtatcttcg taaatgctgg tcagatggtc ggagaggcag taggtggagc gtttaagagt
   113221 gcggtcaatg cggttcttgg aacgattgaa aatgtagtca atggcttcat cggaatgatt
   113281 aatggagttt taggcgttgt cagaaactta cctggtctag gatgggttgg tagtgtaagt
   113341 acagttagcc tccctcgtct tgcccgtggt ggtatcgtcg atagtccaac aatcgccatg
   113401 attggtgaag ctggtaaaga ggcggtcgta ccacttgaaa atacaggatt tatccaaaca
   113461 cttggacgag tagtcagcag tgcggtagta aatgccatgg ctggtgttag tccacaaggt
   113521 ggattctctg gcgacggcga catcgttatc caaatcgcag gccatgagtt cggacgggta
   113581 gccatccaag aaataaacaa ggaacatgaa cgagcaggtc aaaccttgct caagatttag
   113641 gaggttaaat ggcacaattg acaatcaatg gagtggctgt gaagcctccc aaatattttc
   113701 aagtcggtat tcaagatatc gatggagaga cagggcgtaa tgccaatggc gacatgatgc
   113761 gtgaccgtat cacgaccaaa cgcaaactag actgtgaatg gggtatgatg actcagggag
   113821 aattaagtca gcttttacat gctgtatcat ctgaattttt tgaggtatct tatccagacc
   113881 ccatggatgg ccaagtcaca aagactttct atgtcggtga taggacagct cctagctata
   113941 cctttactga gaagtttaaa ccttggtctg gcgctaaatt taatctggta gagaggtaag
   114001 aaaatggacg ctttaactag acgacaattt gacagagcca tgtttgccaa ggaaaggacg
   114061 ctggctattc gtgttggtga ttatgcttca cgggatatca aagaggctag ttttgagtat
   114121 ggctacatta agggcgatac ttataagcct ggtggaacct gcgctggtag cggtaaaatt
   114181 acctttacca gtatcattac cacgttcaat aagctggata ccctgcaccc tgagattggt
   114241 ctactggttg gggataccta ccagtgggtc aagatggggg aatacttcat caacgatatt
   114301 gagattgacc gaaaccgaaa cactaccaca cttgaactta tggatggtat gtttaagctc
   114361 aatcgtgagt atgtgacaga tttgcatttc ccagctgaag tacgagaggt tattcaggaa
   114421 atctgcctga aaacaggcat tgagttagcg aatgactatt tcggaatcag cgcgatgcgt
   114481 tatcatattg agcaagttcc tgagggcaag aaactttcct ttagggatat gctgagcgct
   114541 atgactcaga tgattgggat gtcttgtttc ttcaacagag aaggcaagat ggaaatccgt
   114601 gatttgactg agtccaatat cacgatcaac gctgacagtt acttcttgca tggcttgacc
   114661 aagagtgaga ttgagtatca gatagctggt atcacttgta agacggacaa gaagtctctg
   114721 acggtcggta tgaagacagg ccggtctttg gaactggaca atgtcttcat gacccagagt
   114781 gctttaaatg acctgtatta caaactgaaa aacctaactt actatccgta taatctcaac
   114841 taccaagggc atttactgct tgaggttggg cagtgggtaa ccattcagac caataagaaa
   114901 gagactttta aagttcctgt cttaagtcag agctttactt tcaaaggtgg tctgagagga
   114961 cgtatcagcg cagatagtaa agctggaaat gatactcagt attcttacga gggaacgatt
   115021 accaagcaga taaagcaaca agatggcgtt gaagcaaaaa tccaagcgca gattgaagca
   115081 gcagataaag attttgacca aaaggtcgac aaaatcaaaa aagactttaa cgatcaagta
   115141 gaacttgcca aagccagagc tgaagaagtc aagagagaac tgtctgacac tatcaatcag
   115201 cgctttaata gctttgacaa cgggccattg aaagaaacta agcgcaaggc tgaggaagct
   115261 ttgcgaaa
//
LOCUS       DBERMR010000002        74793 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.002, whole genome shotgun sequence.
ACCESSION   DBERMR010000002 DBERMR010000000
VERSION     DBERMR010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 74793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74793
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.002"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            184..6882
                     /gene="bgaA"
                     /locus_tag="ACL8A1_000149"
     CDS             184..6882
                     /gene="bgaA"
                     /locus_tag="ACL8A1_000149"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000515226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored adhesin/beta-galactosidase BgaA"
                     /protein_id="HGS1201008.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEVNQMIEDRKVDFNQNWYF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYHPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKSAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVARVRTLIGKEPKMPKTVGFVYSDGSREKLPVTWSQ
                     VDVSQAGVVTVKGTANGREVEAHVEVLAIAKELPTVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEITEVDKAKLSVAGSRIQMTGQLAGETIHATLVVEEGNAAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFVQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLKAEYEGAKGQVDLTIQANTEKKIAQSIRP
                     VNVVTDLHQKPTLPTTVTVEYDKGFPKALKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRSNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAIRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQVRLLQVGQALELPTKVPVYFTGKDGYETKDLTVEWEEVPAEN
                     LTKAGQFTVRGRVLGSNLVAEVTVRVTDKLGETLSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQGKVQFFA
                     DSGTDAPSKLVLERYVGPEFEMPTYYSNYQAYDADHPFNNPENWEAVHYRADKDIEAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IHLTKEKPVSEKTVAAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGK
                     ERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSAASQAAVAGLALLGLSAGLVVTKGKKED"
     gene            complement(7131..7361)
                     /locus_tag="ACL8A1_000150"
     CDS             complement(7131..7361)
                     /locus_tag="ACL8A1_000150"
                     /inference="COORDINATES: protein motif:HMM:NF025126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGS1201009.1"
                     /translation="MNERYQNLKAKECQALLSPQGRQIFAQRKIDVEPVFGQLKACLG
                     YKRCNLRGKRQVRIDMGLVLMANNLLKHSEMK"
     gene            complement(7330..7743)
                     /locus_tag="ACL8A1_000151"
     CDS             complement(7330..7743)
                     /locus_tag="ACL8A1_000151"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201010.1"
                     /translation="MTLDCLFIDGTKIEANANKYSFVWKKTTEKFSAKLQEQIQVYFQ
                     EEITPLLIKYAMFDKEQKRGYKQSAKNLANWHYNDKEDSYTHPDGWYYRFHHTKHQKT
                     QTDFQQEIKVYYADEPESAPQKGAIYERTLSKLES"
     gene            complement(7743..7988)
                     /locus_tag="ACL8A1_000152"
     CDS             complement(7743..7988)
                     /locus_tag="ACL8A1_000152"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGS1201011.1"
                     /translation="MHIHYNTNQTTLPLEISSFLPQDHLVFTIEKVVNTLEEHHFYAF
                     YHAFDRPSYHPKMLVSTLLFVYSQGIFSGRKIEKWKS"
     gene            complement(8140..8499)
                     /locus_tag="ACL8A1_000153"
     CDS             complement(8140..8499)
                     /locus_tag="ACL8A1_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HGS1201012.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKAFTHNFLPRLGLALSILAIILVFFFLLKKRSFYYPKFIKFFWRTGFL
                     LTLIMYIEMVVELFLMK"
     gene            8591..9548
                     /locus_tag="ACL8A1_000154"
                     /pseudo
     CDS             8591..9548
                     /locus_tag="ACL8A1_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; frameshifted;
                     internal stop; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            9532..10089
                     /locus_tag="ACL8A1_000155"
     CDS             9532..10089
                     /locus_tag="ACL8A1_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGS1201013.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            10082..10336
                     /locus_tag="ACL8A1_000156"
     CDS             10082..10336
                     /locus_tag="ACL8A1_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201014.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTHHERSVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            10348..12402
                     /locus_tag="ACL8A1_000157"
     CDS             10348..12402
                     /locus_tag="ACL8A1_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HGS1201015.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNRYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGSFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            12581..13441
                     /locus_tag="ACL8A1_000158"
     CDS             12581..13441
                     /locus_tag="ACL8A1_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HGS1201016.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLGFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            13652..14362
                     /gene="ccdA2"
                     /locus_tag="ACL8A1_000159"
     CDS             13652..14362
                     /gene="ccdA2"
                     /locus_tag="ACL8A1_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HGS1201017.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYILKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            14382..14948
                     /locus_tag="ACL8A1_000160"
     CDS             14382..14948
                     /locus_tag="ACL8A1_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HGS1201018.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVTDFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            14959..16071
                     /gene="msrB"
                     /locus_tag="ACL8A1_000161"
     CDS             14959..16071
                     /gene="msrB"
                     /locus_tag="ACL8A1_000161"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HGS1201019.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            16294..17031
                     /locus_tag="ACL8A1_000162"
     CDS             16294..17031
                     /locus_tag="ACL8A1_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGS1201020.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQTENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            17028..18719
                     /locus_tag="ACL8A1_000163"
     CDS             17028..18719
                     /locus_tag="ACL8A1_000163"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGS1201021.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            18835..19398
                     /locus_tag="ACL8A1_000164"
     CDS             18835..19398
                     /locus_tag="ACL8A1_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HGS1201022.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILSSRGIHLAVQGCEHVNRALVVERQVAEQFGLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKS"
     gene            19420..24873
                     /locus_tag="ACL8A1_000165"
     CDS             19420..24873
                     /locus_tag="ACL8A1_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZmpA/ZmpB/ZmpC family metallo-endopeptidase"
                     /protein_id="HGS1201023.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAGAVDPVATLALASREGVVEMDGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKTEESPKEEPKSEIK
                     PTDDTLPKVEEGKEASAEPAPVEEVGGEVESKPEEKVAVKPESQLSDKPAEESKVEPP
                     VEQAKVPEQPVQPTQAEQPSTPKESSQEDNSKEDRGAEETPKQEDEQPAEAPEIKVEE
                     PVESKEETVNQPVEQPKVETPAVEKQTEPTEEPKVEVTTVETREEVIPFETKEQEDDT
                     LKRGTRQVVQKGVEGKKQITETYKTIRGEKTNEAPIVKETVIEQPQDEIIKKGTKGLE
                     KPTLQWANTEKDVLKKSATASYTLTKPAGVEIKSIKLALKDKDGQLVKEVTVAENNLN
                     ATLDKLKYYQGYTLSTTMVYDRGEGEETEKLEDKQIQLDLKKVEIKNIKETSLMNVDA
                     EGNETDKSLLSEKPTDVSQLYLRVTTHDNKVTRLAVSSVEEVVVDGKTLYKVVAKAPD
                     LVQRRADDTLSEEYVHYFEKQLPKVNNVYYNFNELVKDMQANPTGEFKLGADLNAANV
                     KPNGKSYVTKPFKGKLLSNDGGRFTIHNIERPLFANIEGGKIHDINLANVNINMPWAD
                     KVAPIANVIKNNATIENVKVTGNVLGKDWVSGFIDKIDGSGKLINVAFIGNVTSVGTG
                     GNFLTGIVGENWKGYVERAYVDAHIKGKRAKAAGIAYWSQNQGNNFTIGSEGAIKKSV
                     VKGTIDVEKPIEVGGAVGSFTYHGSIEDTVSMMKVKNGEIFYGSKDIDDDPYYTGNHV
                     NRNYVVIGVSEGTSTYRYSNQHNRIKPITQSEADVKIAETAITADKFTITDPIVNKLN
                     ALTTRDNEYRTTQDYEATREQAYRNIEKLQPLYNKEWIVNQGNKLVEGSNLLTKEVLS
                     VTGIKSGQFVTDLSDIDKIMIHYSDGAKEELNVTRQESNVQQVREYSITDLDILYTPN
                     MVEKDRAQLMTDVKSKLSSVELESDGVRQLLVKRDTKKDANANSVGRQNGYIRDLFLE
                     ESFSEVKANLDKLVKQILENEDHQLNDNELAERALLKKVEDNKAKIMMGLAYLNQYYA
                     FKYDELSIKDIMMFKPDFYGKTASVIDRLINIGSAENNLKGDRTQDAYRGIISGATGK
                     GSLHDFLTYNMKLFTNETDINVWFKKAIEKNAYVVEQPSTNSAFANKKYRLYEGINNG
                     QHGRMILPLLNLKNAHLFMISTYNTISFSSFEKYGKDTDEKREKFKSEINKRAKEQVN
                     YLDFWSRLATDNVRDKLLKSQNVVPTPVWDNHNSPNGWASRHGHIDGKPDYAPIREFF
                     GRINKYHGYKYGYGAYAYIFAAPQPMDAVYFVMTDLISDFGTSAFTHETTHVNDRMAY
                     YGGHWHREGTDLEAFAQGMLQTPSVSNPNGEYGALGLNMAYERQNDGNQWYNTNPNDL
                     TTRAEIDNYMKGFNDTLMLLDYLEGEAVLNKANQDLNNAWFKKVDKQLRGASTKNQYD
                     KVRDLTADEKNITLNSVDDLVDNNFMTKHGPGNNVYDPTGFGTAYVTVPITAGIYGGN
                     TSEGAPGSMSFKHNTFRMWGYYGYEKGFLNYASNMLKNESRQAGHNTLGDDFIIKKVS
                     DNKFSTLEDWKKAYFKEVVDKAKAGFNPVTIDSTTYSSYDDLKNAFAAAVEKDKATLK
                     NGSVKSDNTVALKEKIYKKLLQQTDSFKTSIFR"
     gene            24934..26655
                     /gene="pabB"
                     /locus_tag="ACL8A1_000166"
     CDS             24934..26655
                     /gene="pabB"
                     /locus_tag="ACL8A1_000166"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HGS1201024.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVEEVADLLAQVENYQ
                     TQGYYVVGYVSYEAAPAFEEKLAVHKVPLLGEYLLYFTVHDRVETSPIPLTYEDIDLP
                     SNWQEVTSAEDYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLSANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANRDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRSHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQHLLETGQVEEKVLTLADLTQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            26656..27273
                     /locus_tag="ACL8A1_000167"
     CDS             26656..27273
                     /locus_tag="ACL8A1_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGS1201025.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALTLELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKTLMVGVSEELKTLDLTDIAGTCSYPTLLICGSKDKPNL
                     SSMKALHRLLTDSQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            27323..28321
                     /locus_tag="ACL8A1_000168"
     CDS             27323..28321
                     /locus_tag="ACL8A1_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HGS1201026.1"
                     /translation="MNKRLFLKMSLVTLPILALFSQPVLAEENIHFSSCKEAWANGYS
                     DIHEGEPGYSAKLDRDHDGVACELKNAPKGAFKAKQSTAIQINTSSATTSGWVKQDGA
                     WYYFDGNGNLVKNAWQGSYYLKADGKMAQSEWIYDSSYQAWYYLKSDGSYAKNAWQGA
                     YYLKSNGKMAQGEWVYDSSYQAWYYLKSDGSYARNAWQGNYYLKSDGKMAKGEWVYDA
                     TYQAWYYLTSDGSYAYSTWQGNYYLKSDGKMAVNEWVDGGRYYVGADGVWKEGQASTV
                     SSSNDSNSEYSAALGKAKSYNSLFHMSKKRMYRQLTSDFDKFSNDAAQYAIDHLDD"
     gene            28448..29407
                     /locus_tag="ACL8A1_000169"
     CDS             28448..29407
                     /locus_tag="ACL8A1_000169"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HGS1201027.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDREKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            29504..30343
                     /locus_tag="ACL8A1_000170"
     CDS             29504..30343
                     /locus_tag="ACL8A1_000170"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HGS1201028.1"
                     /translation="MTKADTIFKENIECILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(30391..30561)
                     /locus_tag="ACL8A1_000171"
     CDS             complement(30391..30561)
                     /locus_tag="ACL8A1_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HGS1201029.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            30682..31566
                     /gene="miaA"
                     /locus_tag="ACL8A1_000172"
     CDS             30682..31566
                     /gene="miaA"
                     /locus_tag="ACL8A1_000172"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HGS1201030.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEDIQSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQETLYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTFEEASESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            31559..32797
                     /gene="hflX"
                     /locus_tag="ACL8A1_000173"
     CDS             31559..32797
                     /gene="hflX"
                     /locus_tag="ACL8A1_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HGS1201031.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            32790..33413
                     /locus_tag="ACL8A1_000174"
     CDS             32790..33413
                     /locus_tag="ACL8A1_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HGS1201032.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGFGRIFSENEEKISDDLLFEIAQIFSHQLVFEESGKIYMKPVKDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            33428..34357
                     /gene="rnz"
                     /locus_tag="ACL8A1_000175"
     CDS             33428..34357
                     /gene="rnz"
                     /locus_tag="ACL8A1_000175"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HGS1201033.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            34378..35133
                     /locus_tag="ACL8A1_000176"
     CDS             34378..35133
                     /locus_tag="ACL8A1_000176"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HGS1201034.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(35187..36095)
                     /locus_tag="ACL8A1_000177"
     CDS             complement(35187..36095)
                     /locus_tag="ACL8A1_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGS1201035.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            36159..36428
                     /locus_tag="ACL8A1_000178"
     CDS             36159..36428
                     /locus_tag="ACL8A1_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HGS1201036.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            36428..36808
                     /locus_tag="ACL8A1_000179"
     CDS             36428..36808
                     /locus_tag="ACL8A1_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HGS1201037.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILRARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            36897..37124
                     /locus_tag="ACL8A1_000180"
     CDS             36897..37124
                     /locus_tag="ACL8A1_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201038.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLDYKIEKILNILGIVISVVNWIVAIALIFR"
     gene            complement(37164..37889)
                     /locus_tag="ACL8A1_000181"
     CDS             complement(37164..37889)
                     /locus_tag="ACL8A1_000181"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HGS1201039.1"
                     /translation="MRLDNLLAQEKISRKAMKQALFKGDILVDSCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSISQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     EGNYRPLNQKELQIIKNYLEMSR"
     gene            38051..39892
                     /gene="typA"
                     /locus_tag="ACL8A1_000182"
     CDS             38051..39892
                     /gene="typA"
                     /locus_tag="ACL8A1_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HGS1201040.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPIDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            39912..40166
                     /locus_tag="ACL8A1_000183"
     CDS             39912..40166
                     /locus_tag="ACL8A1_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HGS1201041.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            40530..40829
                     /locus_tag="ACL8A1_000184"
                     /pseudo
     CDS             40530..40829
                     /locus_tag="ACL8A1_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
     gene            40900..42921
                     /locus_tag="ACL8A1_000185"
     CDS             40900..42921
                     /locus_tag="ACL8A1_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HGS1201042.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLNYKALVPVIKGR
                     LPLKRLMILTLLCQLVAVFTVGYAVKTGLTSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNSGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKVGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASLFILSSRDLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            42923..43564
                     /locus_tag="ACL8A1_000186"
     CDS             42923..43564
                     /locus_tag="ACL8A1_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1201043.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSIFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            43668..45020
                     /gene="murD"
                     /locus_tag="ACL8A1_000187"
     CDS             43668..45020
                     /gene="murD"
                     /locus_tag="ACL8A1_000187"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HGS1201044.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQCLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYSNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIATD
                     KNTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGLFEEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLASKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            45022..46080
                     /locus_tag="ACL8A1_000188"
     CDS             45022..46080
                     /locus_tag="ACL8A1_000188"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HGS1201045.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VTKVSDQKNPEPDELVDIQSHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            46090..47310
                     /gene="divIB"
                     /locus_tag="ACL8A1_000189"
     CDS             46090..47310
                     /gene="divIB"
                     /locus_tag="ACL8A1_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HGS1201046.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEESES
                     KEKEEQDKKLAKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQVSGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVVDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKRLEEEQKKQEE
                     ESNRNQTTQRSSRR"
     gene            47543..48244
                     /gene="pyrF"
                     /locus_tag="ACL8A1_000190"
     CDS             47543..48244
                     /gene="pyrF"
                     /locus_tag="ACL8A1_000190"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HGS1201047.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            48278..48910
                     /gene="pyrE"
                     /locus_tag="ACL8A1_000191"
     CDS             48278..48910
                     /gene="pyrE"
                     /locus_tag="ACL8A1_000191"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HGS1201048.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMALPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            49371..49493
                     /locus_tag="ACL8A1_000192"
     CDS             49371..49493
                     /locus_tag="ACL8A1_000192"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201049.1"
                     /translation="MYYNGGGDNYIGSGTRLAMAPQFGNHVRIHTSGSWSPDSY"
     gene            49589..50353
                     /locus_tag="ACL8A1_000193"
     CDS             49589..50353
                     /locus_tag="ACL8A1_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201050.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKKYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQLLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLAFGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            50353..51105
                     /locus_tag="ACL8A1_000194"
     CDS             50353..51105
                     /locus_tag="ACL8A1_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201051.1"
                     /translation="MKQFVQFYKKDFLSVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLDNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            51115..51813
                     /locus_tag="ACL8A1_000195"
     CDS             51115..51813
                     /locus_tag="ACL8A1_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201052.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQKRRFIE"
     gene            51810..52457
                     /locus_tag="ACL8A1_000196"
     CDS             51810..52457
                     /locus_tag="ACL8A1_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS1201053.1"
                     /translation="MNEIITLKNIQLELKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(52544..53410)
                     /locus_tag="ACL8A1_000197"
     CDS             complement(52544..53410)
                     /locus_tag="ACL8A1_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS1201054.1"
                     /translation="MKLFKPLLTVLALAFALIFITACSSGGNAGPSSGKTTAKARTID
                     ENKKSGELRIAVFGDKKPFGYVDNDGSYQGYDIELGNQLAQDLGVKVKYISVDAANRA
                     EYLISNKVDITLANFTVTDERKKQVDFALPYMKVSLGVVSPKTGLITDVKQLEGKTLI
                     VTKGTTAETYFEKNHPEIKLQKYDQYSDSYQALLDGRGDAFSTDNTEVLAWALENKGF
                     EVGITSLGDPDTIAAAVQKGNQELLDFINKDIEKLGKENFFHKAYEKTLHPTYGDAAK
                     ADDLVVEGGKVD"
     gene            complement(53422..54179)
                     /locus_tag="ACL8A1_000198"
                     /pseudo
     CDS             complement(53422..54179)
                     /locus_tag="ACL8A1_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            complement(54180..54857)
                     /locus_tag="ACL8A1_000199"
     CDS             complement(54180..54857)
                     /locus_tag="ACL8A1_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGS1201055.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIEISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLDFTRIMPQLVLLFIVYFGLARNFNINISGETSAIIVFTLW
                     GTAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTH
                     LEKHWRN"
     gene            complement(54817..55437)
                     /locus_tag="ACL8A1_000200"
     CDS             complement(54817..55437)
                     /locus_tag="ACL8A1_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGS1201056.1"
                     /translation="MYIAVWGILGSFLLGLIVSIIRHYRIPVLAQVATAYIELSRNTP
                     LLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMAESFRSGLEAISQTQQEIGLA
                     IGLTPLQVFRYVVLPQATAVALPSFSANVIFLIKETSVFSAVALADLMYVAKDLIGLY
                     YETDIALAMLVVAYLIMLLPISLVFSWIERRLRHAGFGNPSTLSGK"
     gene            55470..55604
                     /locus_tag="ACL8A1_000201"
                     /pseudo
     CDS             55470..55604
                     /locus_tag="ACL8A1_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            55744..57234
                     /gene="lysS"
                     /locus_tag="ACL8A1_000202"
     CDS             55744..57234
                     /gene="lysS"
                     /locus_tag="ACL8A1_000202"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HGS1201057.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            57858..58994
                     /gene="lctO"
                     /locus_tag="ACL8A1_000203"
     CDS             57858..58994
                     /gene="lctO"
                     /locus_tag="ACL8A1_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HGS1201058.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     regulatory      59248..59336
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            59551..60219
                     /locus_tag="ACL8A1_000204"
     CDS             59551..60219
                     /locus_tag="ACL8A1_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HGS1201059.1"
                     /translation="METQDYAFQPGLTVEELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     gene            60462..61244
                     /gene="thiM"
                     /locus_tag="ACL8A1_000205"
     CDS             60462..61244
                     /gene="thiM"
                     /locus_tag="ACL8A1_000205"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HGS1201060.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALSVSPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKKFKRVK"
     gene            61246..61875
                     /gene="thiE"
                     /locus_tag="ACL8A1_000206"
     CDS             61246..61875
                     /gene="thiE"
                     /locus_tag="ACL8A1_000206"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HGS1201061.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFDTQSKYDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
     regulatory      62084..62172
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            62433..62993
                     /locus_tag="ACL8A1_000207"
     CDS             62433..62993
                     /locus_tag="ACL8A1_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGS1201062.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNIFSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     gene            62994..64379
                     /locus_tag="ACL8A1_000208"
     CDS             62994..64379
                     /locus_tag="ACL8A1_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS1201063.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQVI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILFQNPNHQFTMENLFEELMFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAAMAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMDRTTCEFGNRSLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGTVLKKKKSLYRELTSIVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            64381..65031
                     /locus_tag="ACL8A1_000209"
     CDS             64381..65031
                     /locus_tag="ACL8A1_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HGS1201064.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRIFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            65042..65734
                     /gene="tenA"
                     /locus_tag="ACL8A1_000210"
     CDS             65042..65734
                     /gene="tenA"
                     /locus_tag="ACL8A1_000210"
                     /EC_number="3.5.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HGS1201065.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKVFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEINDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            65739..66263
                     /gene="thiW"
                     /locus_tag="ACL8A1_000211"
     CDS             65739..66263
                     /gene="thiW"
                     /locus_tag="ACL8A1_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HGS1201066.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFAERID"
     gene            66263..67066
                     /locus_tag="ACL8A1_000212"
     CDS             66263..67066
                     /locus_tag="ACL8A1_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HGS1201067.1"
                     /translation="MQALTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRMAALYANQSSLPMVVDAVGVTTSSIRKS
                     LVKDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLIVSENQVAVLENGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            67059..67691
                     /gene="thiE"
                     /locus_tag="ACL8A1_000213"
     CDS             67059..67691
                     /gene="thiE"
                     /locus_tag="ACL8A1_000213"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HGS1201068.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPV
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLKKLHNILS"
     gene            complement(67901..68692)
                     /gene="thiD"
                     /locus_tag="ACL8A1_000214"
     CDS             complement(67901..68692)
                     /gene="thiD"
                     /locus_tag="ACL8A1_000214"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HGS1201069.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQVRDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESIFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     regulatory      complement(68776..68874)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            69058..69453
                     /locus_tag="ACL8A1_000215"
     CDS             69058..69453
                     /locus_tag="ACL8A1_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HGS1201070.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     SRLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     gene            69464..69835
                     /locus_tag="ACL8A1_000216"
     CDS             69464..69835
                     /locus_tag="ACL8A1_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HGS1201071.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHTNLPMGEEYVVEITPE
                     QAGEFGFACGMNMMHGKMIVE"
     gene            69845..72088
                     /locus_tag="ACL8A1_000217"
     CDS             69845..72088
                     /locus_tag="ACL8A1_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HGS1201072.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTTIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDVIGDEVEVFRLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQTLHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            72295..74070
                     /gene="spxB"
                     /locus_tag="ACL8A1_000218"
     CDS             72295..74070
                     /gene="spxB"
                     /locus_tag="ACL8A1_000218"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HGS1201073.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAISKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            74181..74528
                     /locus_tag="ACL8A1_000219"
     CDS             74181..74528
                     /locus_tag="ACL8A1_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGS1201074.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVGQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(74645..>74793)
                     /locus_tag="ACL8A1_000220"
     CDS             complement(74645..>74793)
                     /locus_tag="ACL8A1_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HGS1201075.1"
                     /translation="RKRGFKNKAFRTLEDVMNQLQDVIQGLEKEVIKSIVNRRWTRML
                     FESR"
BASE COUNT        22700 a        13671 c        16518 g        21904 t
ORIGIN      
        1 agttttgagg ttgtagatga aactgacgaa gtcagtaatc atatctacgg caaggcgaca
       61 ctgacgaagt ttgaattgga ttttcgcaga gtgtaaattg ttgaaactag gttgtcatac
      121 catgtatacc acttgggctt tggtagtagc taactgcact aaatataata taaggagggt
      181 tttatgggga aaggccattg gaatcggaaa agagtttata gcattcgtaa gtttgctgtg
      241 ggagcttgct cagtaatgat tgggacttgt gcagttctat taggaggaaa tatagctgga
      301 gaatctgtag tttatgcgga tgaaacactt attactcata ctgctgagaa acctaaagag
      361 gaaaaaatga tagtagaaga aaaggctgat aaagctttgg aaactaaaaa tgtagttgaa
      421 aggacagaac aaagtgaacc tagttcaact gaggctattg catctgagaa gaaagaagat
      481 gaagccgtaa ctccaaaaga ggaaaaagtg tctgctaaac cggaagaaaa agctccaagg
      541 atagaatcac aagcttcaag tcaagaaaaa ccgctcaagg aagatgctaa agctgtaaca
      601 aatgaagaag tgaatcaaat gattgaagac aggaaagtgg attttaatca aaattggtac
      661 tttaaactca atgcaaattc taaggaagcc attaaacctg atgcagacgt atctacgtgg
      721 aaaaaattag atttaccgta tgactggagt atctttaacg atttcgatca tgaatctcct
      781 gcacaaaatg aaggtggaca gctcaacggt ggggaagctt ggtatcgcaa gactttcaaa
      841 ctagatgaaa aagacctcaa gaaaaatgtt cgccttactt ttgatggcgt ctacatggat
      901 tctcaagttt atgtcaatgg tcagttagtg gggcattatc caaatggtta taaccagttc
      961 tcatacgata tcaccaaata ccttcacaaa gatggtcgtg agaatgtgat tgctgtccat
     1021 gcagtcaaca aacagccaag tagccgttgg tattcaggaa gtggtatcta tcgtgatgtg
     1081 actttacaag tgacagataa ggtgcatgtt gagaaaaatg ggacaactat tttaacacca
     1141 aaacttgaag aacaacaaca tggcaaggtt gaaactcatg tgaccagcaa aatcgtcaat
     1201 acggacgaca aagaccatga acttgtagcc gaatatcaaa tcgttgaacg aggtggtcat
     1261 gctgtaacag gcttagttcg tacagcgagt cgtaccttaa aagcacatga atcaacaagc
     1321 ctagatgcga ttttagaagt tgaaagacca aaactctgga ccgttttaaa tgacaaacct
     1381 gccttgtacg aattgattac gcgtgtttac cgtgacggtc aattggttga tgctaagaag
     1441 gatttgtttg gttaccgtta ctatcactgg actccaaatg aaggtttctc tttgaatggt
     1501 gaacgtatta aattccatgg agtatccttg caccacgacc atggggcgct tggagcagaa
     1561 gaaaactata aagcagaata tcgccgtctc aaacaaatga aggagatggg agttaactcc
     1621 atccgtacaa cccacaaccc tgctagtgag caaaccttgc aaatcgcagc agaactaggt
     1681 ttactcgttc aggaagaggc ttttgatact tggtatggtg gcaagaaacc ttatgactat
     1741 ggacgttttt ttgaaaaaga tgccactcac ccagaagctc gaaaaggtga aaaatggtct
     1801 gattttgacc tacgtaccat ggtcgaaaga ggcaaaaaca accctgctat cttcatgtgg
     1861 tcaattggta atgaaatagg tgaagctaat ggtgatgccc actctttagc aactgttaaa
     1921 cgtttggtca aggttatcaa ggatgttgat aagactcgct atgttaccat gggagcagat
     1981 aaattccgtt tcggtaatgg tagcggaggg catgagaaaa ttgctgatga actcgatgct
     2041 gttggattta actattctga agataattac aaagccctta gagctaagca tccaaaatgg
     2101 ttgatttacg gttcagaaac atcatcagca acccgtacac gaggaagtta ctatcaccct
     2161 gaacgtgaat tgaaacatag caatggacct gagcgtaatt atgaacagtc agattatgga
     2221 aatgatcgtg tgggttgggg gaaaacagca accgcttcat ggacttttga ccgtgacaac
     2281 gctggctatg ctggacagtt tatctggaca ggtacggact atattggtga acctacacca
     2341 tggcacaacc aaaatcaaac tcctgttaag agctcttact ttggtatcgt agatacagcc
     2401 ggcattccaa aacatgactt ctatctctac caaagccaat gggtttctgt taagaagaaa
     2461 ccgatggtac accttcttcc tcactggaac tgggaaaaca aagaattagc atccaaagta
     2521 gctgactcag aaggtaagat tccagttcgt gcttattcga atgcttctag tgtagaatta
     2581 ttcttgaatg gaaaatctct tggtcttaag actttcaata aaaaacaaac cagcgatggg
     2641 cggacttacc aagaaggtgc aaatgctaat gaactttatc ttgaatggaa agttgcctat
     2701 caaccgggta ccttggaagc aattgctcgt gatgaatctg gcaaggaaat tgctcgagat
     2761 aagattacga ctgctggtaa gtcagcggca gttcgtctta ttaaggaaga ccatgcgatt
     2821 gcagcagatg gaaaagactt gacttacatc tactatgaaa ttgttgacag ccaggggaat
     2881 gtggttccaa ctgctaataa tctggttcgc ttccaattgc atggccaagg tcaactggtc
     2941 ggtgtagata acggagaaca agccagccgt gaacgctata aggcgcaagc agatggttct
     3001 tggattcgta aagcatttaa tggtaaaggt gttgccattg tcaaatcaac tgaacaagca
     3061 gggaaattca ccctgactgc ccactctgat ctcttgaaat cgaaccaagt cactgtcttt
     3121 actggtaaaa aagaaggaca agagaagact gttttgggga cagaagtagc aagagttcgt
     3181 acattaattg gtaaagaacc aaaaatgccg aaaacagtag gttttgtata cagcgatggt
     3241 agtcgtgaaa aacttccagt aacttggtct caagtcgatg ttagtcaagc aggagttgtg
     3301 actgttaaag gtacagcaaa tggacgtgag gtagaggctc atgtcgaggt tctagcaatt
     3361 gcgaaagaat taccaacagt taaacgtatt gctccaaata ctgacttgaa ttctgtagac
     3421 aaatctgttt cttatgtttt gactgatgga agtgtacaag agtacgaagt agatagctgg
     3481 gagattacgg aagtagataa agccaaactt tcagtagctg gatcacgtat tcaaatgact
     3541 ggtcagttag ctggagaaac tattcatgca acccttgtgg tagaagaagg aaatgctgca
     3601 gcacctgtag tgccaactgt tactgttgga ggtgaagcag taacaggtct tactagtcga
     3661 caaccaatgc aatatcgtac tctatcttat ggtgcccaat tgccagaagt cacagcaagt
     3721 gctgaaaatg ctgatgtgac agttcttcaa gcaagcgcag caaacggcat gcgtgcaagc
     3781 atatttgttc aacctaaaga tggtggccct cttcaaacct atgcaattca attccttgaa
     3841 gaagcaccaa aaattgctca cttgagcttg caagtggaaa aagctgacag tctcaaagaa
     3901 gaccaaactg tcaaattgtc ggttcgagct cactatcaag atggaacgca agctgtatta
     3961 ccagctgata aagtaacctt ctctacaagt ggtgaagggg aagtcgcaat tcgtaaagga
     4021 atgcttgagt tacataagcc aggagcagtc actctcaaag cggaatatga gggagctaaa
     4081 ggccaagttg atctcactat ccaggccaat actgagaaga agattgcgca atctatccgt
     4141 ccagtaaatg tagtgacaga tttgcatcaa aaacctactc ttccaacaac agtaacggtt
     4201 gagtatgaca aaggtttccc taaagctctc aaagtcactt ggcaagctat tccgaaagaa
     4261 aaactagact cctatcaaac atttgaagta ctaggtaaag ttgaaggaat tgaccttgaa
     4321 gcgcgtgcaa aagtctctgt agaaggtatc gtttcagttg aagaagtcag tgtgacaact
     4381 ccaatcgcag aagcaccaca attaccagaa agcgttcgga catatgattc aaatggtcac
     4441 gtttcatcag ctaaggttgc atgggatgcg attcgtccag agcaatacgc taaggaaggt
     4501 gtctttacag ttaatggtcg cttagaaggt acgcaattaa caactaaact tcatgttcgc
     4561 gtatctgctc aaactgagca aggtgcaaac atttctgacc aatggaccgg ttcagaattg
     4621 ccacttgcct ttgcttcaga ttcaaatcca agcgacccag tttcaaatgt taatgacaag
     4681 ctcatttcct acaataacca accagccaat cgttggacaa actggaatcg tagtaatcca
     4741 gaagcttcag tcggtgttct gtttggagat tcaggtatct tgagtaaacg ctccgttgat
     4801 aatctaagtg tcggattcca cgaagaccat ggagttggtg caccgaagtc ttatgtgatt
     4861 gagtattatg ttggtaagac tgtcccaaca gctcctaaaa accctagttt tgttggtaat
     4921 gaggaccatg tctttaatga ttctgccaac tggaaaccag ttactaatct aaaagcccct
     4981 gctcaactca aggctggaga aatgaaccac tttagctttg ataaagttga aacctatgct
     5041 attcgtattc gcatggttaa agcagataac aagcgtggaa cgtctatcac agaggtacaa
     5101 atctttgcga aacaagttgc ggcagccaaa caaggacaaa caagaatcca agttgacggc
     5161 aaagacttag caaacttcaa ccctgatttg acagactact accttgagtc tgtagatgga
     5221 aaagttccgg cagtcacagc aagtgttagc aacaatggtc tcgctaccgt cgttccaagc
     5281 gttcgtgaag gtgagccagt tcgtgtcatc gcgaaagctg aaaatggcga catcttagga
     5341 gaataccgtc tgcacttcac taaggataag aacttacttt ctcataaacc agttgctgcg
     5401 gttaaacaag ttcgcttgct acaagtaggt caagcacttg aattgccgac taaggttcca
     5461 gtttacttca caggtaaaga cggctacgaa acaaaagacc tgacagttga atgggaagaa
     5521 gttccagcgg aaaatctgac aaaagcaggt caatttactg ttcgaggccg tgtccttggt
     5581 agtaaccttg ttgctgaggt cactgtacga gtgacagaca aacttggtga gactctttca
     5641 gataacccta actatgatga aaacagtaac caggcctttg cttcagcaac caatgatatt
     5701 gacaaaaact ctcatgaccg cgttgactat ctcaatgacg gagatcattc agaaaatcgt
     5761 cgttggacaa actggtcacc aacaccatct tctaatccag aagtatcagc gggtgtgatc
     5821 ttccgtgaaa atggtaagat tgtagaacgg actgttgcac aaggaaaagt tcagttcttt
     5881 gcagatagtg gtacggatgc accatctaaa ctcgttttag aacgctatgt cggtccagag
     5941 tttgaaatgc caacctacta ttcaaactac caagcctacg acgcagacca tccattcaac
     6001 aatccagaaa attgggaagc tgtgcattat cgtgcggata aagacattga agccggtgat
     6061 gaaattaacg ttacctttaa agctgtcaaa gccaaagcca tgagatggcg tatggagcgt
     6121 aaagctgaca agagcggtgt tgcgatgatt gagatgacct tcctggcacc gagtgaattg
     6181 cctcaagaaa gtacgcaatc gaagattctt gtagatggaa aagaacttgc tgatttcgct
     6241 gaaaatcgtc aagactatca aattacctat aaaggtcaac ggccaaaagt ctcagttgaa
     6301 gaaaacaatc aagtagcttc aactgtggta gatagtggag aagatagcct tccagtactt
     6361 gttcgcctcg tttcagaaag tggaaaacaa gtcaaggaat accgtatcca cttgactaag
     6421 gaaaaaccag tttctgagaa gacagttgct gctgtacaag aagatcttcc aaaactcgaa
     6481 tttgttgaaa aagatttggc atacaagaca gttgagaaaa aagattcaac actgtatcta
     6541 ggtgaaactc gtgtagaaca agaaggaaaa actggtaaag aacgtatctt tacagcgatt
     6601 aatcctgatg gaagtaagga agaaaaactc cgtgaagtgg tagaagctcc gacagaccgc
     6661 atcgtcttgg ttggaaccaa accagtagct caagaagcta aaaaaccaca agtgtcagaa
     6721 aaagcagata caaaaccaat tgattcaagt gaagctagtc aaactaataa agcccagtta
     6781 ccaaatacag gtagtgcggc aagccaagca gcagtagcag gtttagctct tctaggtttg
     6841 agtgcaggat tagtagttac taaaggtaaa aaagaagact agaaattttg ataccttctt
     6901 tatcattaaa gaccaaagaa aaactcttgt cctaaataga agataaagag tctgggacaa
     6961 aatagttctc aaccacaaaa aaagctagat atttccaatt gcggaactct agctttttaa
     7021 ttttgagtcg ttctcttatt gtatagtaga ttgaaactag agtagtacac ttctacttct
     7081 aaaacattgt tagaaatcga tttgactgtc cttatcgatt tgtcctgttc ttatttcatt
     7141 tcactatgtt ttaggaggtt attggccata agtaccaatc ccatgtcaat tctcacttga
     7201 cgcttgcctc tcagattaca tctcttgtaa cccaaacaag cctttaactg cccaaagaca
     7261 ggttccacat caatcttgcg ttgagcgaaa atctgtctac cttggggaga taaaagtgcc
     7321 tgacattctt tagctttcaa gttttgatag cgttcgttca tatatagccc ccttttgagg
     7381 ggctgattca ggttcgtcgg catagtaaac cttgatttcc tgttgaaagt ctgtctgtgt
     7441 tttctgatgt ttggtatggt gaaaacgata ataccagcca tcaggatgtg tgtagctatc
     7501 ctccttgtca ttatagtgcc aattcgctaa gtttttagct gactgtttat accctctctt
     7561 ttgttcctta tcaaacatgg catatttaat cagaagggga gtgatttctt cttgaaaata
     7621 gacttgtatc tgttcttgaa gtttggcgga gaatttctct gtcgttttct tccacacaaa
     7681 actatacttg ttggcattgg cttcaatctt agttccgtca ataaacaaac aatctaaggt
     7741 cactaactct tccatttttc aatttttcga ccagagaaaa tcccttgtga atagacaaat
     7801 agaagagtag atacaagcat tttagggtga taagacgggc gatcaaaggc atgatagaag
     7861 gcgtagaagt gatgttcctc caaggtattc accacttttt caatagtaaa gacgagatgg
     7921 tcttgtggta agaaggaact gatttctagt ggtaaagttg tttgatttgt gttatagtga
     7981 atatgcatgg gatagccttt ctaaatggtt tattgtgcaa ttctatttta ccaagactat
     8041 tgtcccagac tcttttcttt gtataaaatt gtatagtgtt attgtctata ctattttgta
     8101 gagaaagcaa aaatccctag aaaatgctta gggattcgac tatttcatta agaacaattc
     8161 aacaaccatc tctatataca tgataagggt taataaaaat cctgtacgcc agaagaattt
     8221 gatgaattta ggatagtaaa agctgcgttt tttcaaaagg aagaaaaaga cgagaataat
     8281 ggctaggatc gagagggcta gccccagtct agggaggaaa ttatgggtaa aggctttagc
     8341 tgtaatcagg taatattcaa ataccaagac cggaaaagcc aaatccgcaa agtttcgtcc
     8401 tagttttttt aatctaaaaa gtttggttag gataatgcag acgactaagg tcagtatcaa
     8461 taataaaata gatgctaatt tcattaaaat catacccata ttgtatcata aaaaaaggct
     8521 aatggaaacg agaaacagag gaatttatag gaaagtcagg cttttgagat tgtgggtgat
     8581 ttttgttata atgaaagtta taaaatctta taataccttg aatgattatt atcgaaaact
     8641 ctttggagaa aagactttta aagtccctat tgatgcggga tttgactgtc ccaatcgtga
     8701 tggaactgtg gctcatggag gctgtacttt ttgtacggtt tcaggttctg gagatactat
     8761 tgtagcccca gatgcgccta tccgtgagca attttataag gaaattgact ttatgcaccg
     8821 taagtggccg gatgttcaga agtatctggt ttattttcaa aattttacca acacccatga
     8881 aaaggtggaa gttattcgag agcgttatga acaggctatc aatgagccag gtgtggtagg
     8941 aatcaatatt ggaacgcgcc cagactgttt accagacgaa accatcgaat atttggctga
     9001 gttatcggag tgcatgcatg tgacggttga attaggcttg cagaccactt atgaagcaac
     9061 ctctgacctg attaaccgtg tgcattctta tgaattgtag gtggaaacgg tcaagcgttt
     9121 gagaaagtat cccaagattg agattgtttc cccatttgat caatggtttg cctggtgaaa
     9181 cccatgagat gatggttgaa aatgtccgcc gttgtgtcac ggataacgat attcaaggga
     9241 ttaaactgca tttgctccat cttatgacaa atactcgtat gcaacgagat taccatgagg
     9301 gacgtttaca attgatgagt caggacgaat atgtcagggt catctgtgac caaatggaaa
     9361 ttattcccaa acatatcgtc atccatcgaa ttacaggaga tgcgcctagg gatatgctga
     9421 ttggtcctat gtggagcctc aaaaaatggg aagtcctcaa cagcattgag atggagatgc
     9481 gacgtcgtgg aagtgttcaa ggatgcaagg ctgtaaaaca ggagtttgaa aatgaaaaga
     9541 ccacttgaga tggcacatga ttttttggct gaggtcgtga caaaagagga tgtcgtagtg
     9601 gatgcgacta tgggaaatgg tcatgacacg ctttttttag ccaagctagc caagcaagtc
     9661 tatgcctttg atattcagaa gcaagccttg gaaaagaccc aagagcgttt gcatcaggct
     9721 ggcttgacaa atgcccagtt aatcttgcaa ggccatgaga cactggacca gtttgtgaca
     9781 aaagctaagg cagggatttt taatctgggc tatttgccgt cagctgataa gtctgtcatc
     9841 acccgaccgc agacaacgat tgaggcatta gaaaagctat gtggcttact tgtcaaaggt
     9901 ggacgaattg ctattatgat ttactatggt catgaaggag gcgacctcga gagagatgct
     9961 gtcttggatt ttgtgatcca gttgaaccaa caagagtaca cagctgccat ttaccgaact
    10021 ttaaaccaag tcaacaaccc gccgttttta gtgatgattg aaaaattaga gagatacaga
    10081 catggataaa caatacctac acgaaaagct ggatgccatg cgccagaatt ttgttgaatc
    10141 aacccaccac gaacgatcgg tgggcgtgct agaccaagca catatgagca aaaaaatgct
    10201 taaaatcaag aaaaaattag ttgctcttga aatggaacgg tgccagagaa aaattgagca
    10261 caaagactgt tccaagattg accaaaaaat caaagagcag aaggagatat ttgaatcctg
    10321 ttgtaaaaaa gattaaggag gacgtgcgtg gaattactga tttacctcat cctattttta
    10381 ctggttttga ttgtctcaag tacaaccaat aagctcctgc cctttttgcc tctccctttg
    10441 gtgcagattc ttttgggaat tgtgattggt ctctttttac ccaatactga ctttcacctt
    10501 aatacggagt tgtttttggc actggttatc ggacccttgc ttttccgaga ggctgaagaa
    10561 gcagatgtta cggctatttt aaaacactgg cgaatcattg tttatctcat atttccagtg
    10621 atttttatct cgaccctgag tttgggtggc ttggcccatc ttctttggtt cagccttccc
    10681 ttggcagctt gcttggctgt tggtgcagcc cttggtccta cggacttggt ggcctttgcc
    10741 tctctttcgg agcgttttag ctttcctaag cgcgtgtcca atattcttaa gggcgaagga
    10801 ctcttgaatg atgcttctgg tttggtggct tttcaggtag ctttgacagc ttggacaact
    10861 ggagcttttt ctctggggca agctagcagt tcgctcatct tttcaatcct aggcggtttt
    10921 ttaattggat ttttaacagc catgaccaac cgcttcctcc ataccttctt gctaagtgtg
    10981 cgcgcaacgg atattgccag tgaactttta ttagaattga gtttgcctct agtgaccttc
    11041 tttctggcag aagaagtcca tgtttcaggg attattgccg tcgtagttgc tggaatttta
    11101 aaggcaagtc gcttcaagaa aatcacgctc ctcgaagccc aagtggatac ggtgaccgag
    11161 acggtctggc atacagtgac ctttatgctc aacggttctg tctttgtgat tttagggatg
    11221 gagttggaaa tgatagcaga acctatcttg accaatccaa tctataatcc tctactttta
    11281 ttgctatctc tcatcgccct tacctttgtc ctctttgtca ttcgttttat tatgatctat
    11341 ggctattatg cctatagaac ccgacgcctc aagaaaaagc taaatagata tatgaaggac
    11401 atgtttctct tgaccttttc aggtgttaag ggaacggtgt cgattgctac gattctcttg
    11461 ataccaagta atttagaaca ggagtatcct ctcttgcttt tccttgttgc aggtgtgacg
    11521 cttgtcagct ttttaacagg tctcttggtc ttgcctcatc tttctgatga agaggaagaa
    11581 agcaaggatt atctcatgca tatcgccatt ttgaatgaag taacgctaga gttggaaaaa
    11641 gagttggaag acaccagaaa taaacttccc ctctatgcgg ctattgacaa ttatcatgga
    11701 cgtattgaaa atctcatttt aagccaagaa aaccaggatg atcaagaaga ttgggctgct
    11761 ttgaaactct tgattcttag tattgaaagt gatggtttgg agcaggccta tgaagagggg
    11821 aacattagca atcgtgctta ccgagtttac caacgttatc tgaaaaatat agaacaagga
    11881 atcaatcgta aacttgcctc aagactgacc tattatttcc ttgtttcttt gaggatttta
    11941 cgttttctcc ttcatgaagt ttttactctt ggaaagacct tccgtagctg gaaggacaag
    12001 gagcaaagcc gtcttcgtgc tcttgattat gaccaaattg cagagctcta tcttgccaat
    12061 acagagatga ttattgaaag tttggaaaac ctgaagggag tctacagacg ctctttgatt
    12121 agttttatgc aggagtctcg tcttcgagaa acagctatta tcagcagtgg ttcctttgtc
    12181 gaacgggtta tcaatcgtgt caaacccaac aatatcgatg aaatgctgag aggctattat
    12241 ctggagcgca agttgatttt cgaatacgaa gaaaaacgat taattacaac taagtatgcc
    12301 aagaaattac gacaaaatgt aaataactta gagaactatt ccttgaagga agctgccaat
    12361 accctgccgt atgatatggt ggaattggta agaagaaatt agttaatact cttcgaaaat
    12421 ctcttcaaac cacgtcagcg tcgccttgga ttatatatgt gactgacttc gtcagtctta
    12481 tctacaacct caaagcaggg ctttgagcaa cctgcggcta gcttcctagt ttgctctttg
    12541 attttcattg agtataagat tataagtgaa ggagtgtgac atgaaaaagt ggtggaaaga
    12601 gctgatagac aagcctttat taaaagcttt tttgcattat tatcaagcat cagatagtga
    12661 gttgaccagt gtcgcagtag cctactattg gttgatttcg attttccccc tgcttttggt
    12721 ggtggtcaat atcctcccct attttcagat tcctgtggga gaatttctgg gctttatgaa
    12781 agacgtcctg cccccaagcc tctatgaagg tgtggaaaaa atagccagag aagtcttgac
    12841 gcaaccttca acaggtttat tgagtttttc tgttttatcg gcgttatgga gtttttcaaa
    12901 atctatgaat ttcttgcaaa aagcttttaa caaggcttat ggcgtcgaaa agagtcgtgg
    12961 acttatttcc catcagatgc taagtctcct agtcagtttt ggcttgcagc tcctctttgc
    13021 gtttgccttg tttttgatct tatttggtca gatgattttg gctttacttg ctcattattg
    13081 gacaaaagat ggcattatct atcaggcttt acaagggttg gcaggtcctc tgatttacgc
    13141 cctcctcttt gctatcttgg tcatgcttta ttattttctt cccaatctat ctaacaggaa
    13201 aattagctat accctaccag gcagtgcctt tgttctctta gtgattttag gtttactaac
    13261 tctgttctct agttatctca attactatgt ccatcatttg gtggatgtcc gaattttagg
    13321 ttccgttctt cttgttgtta tgatgttttg gtttatcttg attgctaaaa ttgttatcct
    13381 aggcgcggtt atcaatgcca gtatgcagag tttgaaagat ccggtcttta aaaaagacta
    13441 actatttatc cattataaaa gcgcatagta tcaagacttt atagaacctt gatactatgc
    13501 gcttttgtgc tttgacagtt ttttgtatag tttttgtcca agatttctaa atcatctgat
    13561 taaattccta ttttccccta tcattgtccc tgttttttcg aaaatggaag gagtataata
    13621 aatctatcaa taaaatgata ggaggaactt tatgggtcat attttctttt ttctaagtgt
    13681 ctttttggca gggattctat ccttcttttc tccttgtatc ttacctttgt taccggtcta
    13741 tacaggagtg ttactagatg ataaggatgg tgctcaggct tctagcggca aattttcaat
    13801 ctcagttact agtttattac gaactctggc ctttatagca ggaatttcct ttatatttat
    13861 tttgttgggc tatggagctg gttttttagg cgatttgctt tatgcttctt ggttccaata
    13921 tcttactggg gcaattatta tccttcttgg tttgcaccaa atggagattc tacactttaa
    13981 ggggctttat aaggaaaaga gactacaact gcaaggacag gggcaaaatg gtaagggcta
    14041 tagtcaggca tttttattgg gcttgacctt tagttttgct tggacgcctt gcgtggggcc
    14101 ggttctgggg tctgttttgg ccttggcggc ttcaggtggt tcaggagctt ggcagggagc
    14161 tggtctcatg ttggtgtata cgctgggctt ggcgctacca ttcttgcttc tagctctgac
    14221 ctctagttat attttgaaac atttccgaaa acttcatccc tatctcggaa tcctcaaaaa
    14281 agtgggtggt tttctcatta ttgtgatggg actcttggtt ctgtttggaa atgcttcaat
    14341 tttaagtcaa ttatttgaat aaaatggaaa ggaatatcaa tatgaaaaaa tggcaaacat
    14401 gtgttcttgg agcaggttcg ctcctttgtt tgacggcttg ttcaggcaag tccgtgacta
    14461 gtgaacacca aacgaaagat gaaatgaaga cggagcagac agctagtaaa acaagcgcag
    14521 ctaaagggaa agaggtgact gattttgaat tgatgggagt agatggcaag acctaccgtt
    14581 tatctgatta caagggcaag aaagtctatc tcaaattctg ggcttcttgg tgttccatct
    14641 gtctggctag tcttccagat acggatgaga ttgctaaaga agctggtgat gactatgtgg
    14701 tcttgacagt agtgtcacca ggacataagg gagagcaatc tgaagcggac tttaagaatt
    14761 ggtataaggg attggattat aaaaatctcc cagtcctagt tgacccatca ggcaaacttt
    14821 tggaaactta tggtgtccgt tcttacccaa cccaagcctt tatagacaaa gaaggcaagc
    14881 tggtcaaaac acatccagga ttcatggaaa aagatgcaat tttgcaaact ttgaaggaat
    14941 tagcctagga ggcgtcttat gaatgataag ttaaaaatct tcttgttgct aggagtattt
    15001 tttctagcca taaccggttt ctatgttcta ttgatacgaa atgcagggca gacagatgcc
    15061 tcgcaaattg aaaaggcggc agttagccaa ggaggaaaag cagtgaaaaa aacagaaatt
    15121 agtaaagacg cagacttgca cgaaatttat ctagctggag gttgtttctg gggagtggag
    15181 gaatatttct cacgtgttcc cggggtgacg gatgccgttt caggctatgc aaatggtaga
    15241 ggagaaacaa ccaagtacga attgattaac caaacaggtc atgcagaaac cgtccatgtc
    15301 acctatgatg ccaagcaaat ttctctcaag gaaatcctgc ttcactattt ccgcattatc
    15361 aatccaacca gcaaaaataa acaaggaaat gatgtgggga cccagtaccg tactggtgtt
    15421 tattacacag atgacaagga tttggaagtg attaaccaag tctttgatga ggtggctaag
    15481 aaatacgatc aacctctagc agttgaaaag gaaaacttga agaattttgt ggtggctgag
    15541 gattaccatc aagactatct caagaaaaat ccaaatggct actgccatat caatgttaat
    15601 caggcggcct atcctgtcat tgatgccagc aaatatccaa aaccaagtga tgaggaattg
    15661 aaaaagaccc tgtcacctga ggagtatgca gttacccagg aaaatcaaac agaacgagct
    15721 ttctcaaacc gttactggga taaatttgaa tccggtatct atgtggatat agcaactggg
    15781 gaacctctct tttcatcaaa agacaaattt gagtctggtt gtggctggcc tagttttacc
    15841 caacccatca gtccagatgt tgtcacctac aaggaagata agtcctacaa tatgacgcgt
    15901 atggaagtgc ggagccgagt aggagattct caccttgggc atgtctttac ggatggtcca
    15961 caggacaagg gcggcttacg ttactgtatc aatagcctct ctatccgctt tattcccaaa
    16021 gaccaaatgg aagaaaaagg ctacgcttat ttactagatt atgttgatta atactcttcg
    16081 aaaatctctt caaaccacgt cagcgtcgcc ttaccgtatg tagggttact gacttcgtca
    16141 gttttattta caacctcaaa gcggtacttt gagaaacctg tggctatctt cctagtttac
    16201 tttttgattt ttattgagta taagaggtct ttcctaagca gttagaggag aattttgcta
    16261 tactgatact agtaagtggt aaaggagcag atcatgacct acacaatctt aatcgtagaa
    16321 gatgaatatc tggtaagaca aggtttgact aaactggtca atgtagcagc ctacgatatg
    16381 gaaatcatcg gtcagactga aaatggaagg caggcttggg aattgatcca aaagcaggtg
    16441 ccagatatca ttttaaccga tatcaacatg cctcatctaa atggcatcca gttggccagt
    16501 ctggtacgag aaacctatcc tcaggttcat ttggtctttt taacaggtta cgatgatttt
    16561 gattatgcct tgtctgctgt caaactaggt gtggacgact acctgctcaa acccttttct
    16621 cgtcaggata ttgaggaaat gttggggaaa atcaaacaaa aactagacaa ggaagagaaa
    16681 gaagagcagt tacaagattt attgactaat aggtttgaag gaaacatggc ccagaaaatc
    16741 cagtctcatc tggctgatag ccaatttagt ttaaagtctt tagccagtga cttaggtttt
    16801 agtccgacct atctgagttc cttgattaag aaagagttgg gcttgccttt tcaggattat
    16861 ctggtgagag aacgtgttaa acaagccaag ctcttgcttt taactacaga tctgaagatt
    16921 tatgagatcg cagagaaggt tggttttgaa gatatgaact attttaccca acgttttaag
    16981 cagattgcag gtgtgacacc tcgtcagttt aagaagggag aagaccgatg aagcgttctt
    17041 ctcttttagt tagaatggtt atttccatct ttctggtctt tctcattctc ctagctctgg
    17101 ttggaacttt ctactatcaa tcaagttctt cagccattga ggccaccatt gagggcaaca
    17161 gccaaacgac catcagccag actagccact ttattcagtc ttatatcaaa aaactagaaa
    17221 ccacctcgac tggtttgacc cagcagacgg atgttctggc ctatgctgag aatcccagtc
    17281 aagacaaggt cgagggaatc cgagatttgt ttttgaccat cttgaagtca gataaggact
    17341 tgaaaactgt tgtgctggtg accaaatctg gtcaggtcat ttctacagat gacagtgtgc
    17401 agatgaaaac ttcctctgat atgatggctg aggattggta ccaaaaggct attcatcagg
    17461 gagctatgcc tgttttgact ccagctcgta aatcagatag tcagtgggtc atttctgtca
    17521 ctcaagaact tgttgatgca aagggagcca atcttggtgt gcttcgtttg gatatttctt
    17581 atgaaactct ggaagcctat ctcaatcaac tccagttggg gcagcagggc tttgccttca
    17641 ttatcaatga aaaccatgaa tttgtctacc atcctcaaca cacagtttat agttcgtcta
    17701 gcaaaatgga ggctatgaaa ccctacatcg agacaggtca gggttatact cctggtcaca
    17761 aatcctacgt cagtcaagag aagattgcag gaactgattg gacggtactt ggcgtgtcat
    17821 cattggaaaa gttagaccag gttcggagtc agctcttgtg gaccttgctt ggggccagtg
    17881 tcacatctct tcttgtctgt ctctgcttag tgtggttcag tcttaaacgc tggattgctc
    17941 ctttgaagga tttgagagaa accatgttgg aaattgcttc tggtgctcaa aatcttcgtg
    18001 ccaaggaagt tggtgcctat gaactgagag aagtaactcg ccaatttaat gccatgttgg
    18061 atcagattga tcagttgatg gtagctattc gtagccagga agaaacgacc cgtcagtacc
    18121 aacttcaagc cctttcgagc cagattaatc cacatttcct ctataacact ttggacacca
    18181 tcatctggat ggctgaattt catgatagtc agcgagtggt gcaggtgacc aagtccttgg
    18241 caacctattt ccgcttggcg ctcaatcaag gcaaggactt gatttgtctc tctgacgaaa
    18301 tcaatcatgt ccgccagtat ctctttatcc agaaacaacg ctatggagat aagctggaat
    18361 acgaaattaa tgaaaatgtt gcctttgata atttagtctt acccaagctg gtcctacaac
    18421 cccttgtaga aaatgctctt taccatggca ttaaggaaaa ggaaggtcag ggccatatta
    18481 aactttctgt ccagaaacag gattcgggat tggtcatccg tattgaggat gatggcgttg
    18541 gcttccaaga tgctggtgat agtagtcaaa gtcaactcaa acgtggggga gttggtcttc
    18601 aaaatgtcga tcaacggctc aaacttcatt ttggagccaa ttaccagatg aagattgatt
    18661 ctagacccca aaaagggacg aaagttgaaa tatatataaa tagaatagaa actagctaac
    18721 tcccagtcaa ttctgggagt tttatgcgta attgggcaaa ctttctaggt tgtctatctt
    18781 gctaaaaaca agaaaaaacg gtatgataga agagtgacaa cagaggtaac agagatgaac
    18841 gagacacaga ttcaaagaga aacacgtcag gtagtggaag atgttttaga aaagaccaat
    18901 ttgaagcagg gggccctttt tgttttaggt ctatcttcta gtgaggtgct aggtggtcag
    18961 attgggaaag aatccagtca agagattggt gagctgatag tggagactat cttgggtatt
    19021 ctaagtagta gaggtatcca tttagcagtt caaggttgtg agcatgtcaa tcgggctctc
    19081 gttgttgaac gtcaggtggc agagcagttt ggtctggaaa ttgtcagtgt ccatcctact
    19141 cttcatgcag gaggttcggg gcagttggca gcctttaagt ttatgcagga tccagttgag
    19201 gttgaattta tcaaggctca tgctggattg gatatcggag acactgcaat tggcatgcat
    19261 gtcaagcatg ttcaggttcc gattcgccct attttgagag agattggtca tgcccatgta
    19321 acggcactgg ctagtcgtcc aaaattaatc ggaggtgcgc gtgcgcacta tccgcaagac
    19381 gctattagaa agtcttgaga atgaataaag gagataaaag tgtttaaaaa agaccgtttt
    19441 tcaattcgta agattaaggg agttgtaggc tctgtatttc ttggaagcct tttgatggct
    19501 ccttctgtag tggacgcagc cacctatcac tatgtaaata aagagattat ttcacaagaa
    19561 gctaaagatt taattcagac aggaaagcct gacaggaatg aagttgtata tggtttggtg
    19621 tatcaaaaag atcagttgcc tcaaacaggg acagaagcat ctgttttgac agcttttggt
    19681 ttgctgactg ttgggagctt gcttttaatc tacaagagaa agaaaattgc tagcgtcttt
    19741 ctagttggag ctatgggatt ggtagttctt cctagtgcag gggctgtaga cccagttgcg
    19801 accctagcgc tggctagtcg agagggtgtt gttgaaatgg atggctatcg ctatgttggt
    19861 tatctatcag gtgacatcct caaaacgctt ggcttggaca ctgttttaga agaaacctca
    19921 gcaaaacctg gagaggtgac tgtagtcgaa gttgagactc ctcaatcaac aacaaatcag
    19981 gagcaagcta ggacagaaaa ccaagtagta gagacagagg aagcacctaa aacagaagaa
    20041 agtccaaagg aagaaccaaa atcggagata aaacctactg acgacaccct tcctaaagta
    20101 gaagagggaa aagaagcttc agcagaacca gctccagttg aagaagtagg tggagaagtt
    20161 gagtcaaaac cagaggaaaa agtagcagtt aagccagaaa gtcaactatc agacaaacca
    20221 gctgaggaat caaaagttga accaccagta gaacaagcaa aagtcccaga acaacccgtg
    20281 caacctacac aagctgagca accaagtaca ccaaaagaat catcacaaga agacaattct
    20341 aaagaagata ggggagcgga agagacaccg aaacaagaag atgaacagcc agcagaagcc
    20401 ccagaaatca aggttgaaga accagtagaa tcaaaagagg agactgttaa tcaacctgtt
    20461 gaacaaccaa aagtggaaac gcctgctgta gaaaaacaaa cggaaccaac agaggaacca
    20521 aaagttgaag taacaacagt tgaaacaaga gaagaagtca ttccttttga aacaaaagaa
    20581 caagaagatg acactttaaa acgcggaaca agacaagtag ttcaaaaagg tgtagaaggt
    20641 aagaaacaaa ttactgaaac ttataaaaca attcgtggag aaaaaacaaa tgaagctcca
    20701 atagttaagg aaacagtaat tgaacaacct caagatgaga ttatcaaaaa aggtacaaaa
    20761 gggttagaaa aaccaacatt acaatgggct aatactgaaa aagatgtctt gaagaagagt
    20821 gctacagcta gttatacatt gactaaacca gctggtgtag aaattaaatc aattaaatta
    20881 gcgctaaaag ataaagatgg acaacttgtt aaagaagtaa cggtagctga aaataattta
    20941 aatgctactt tagataaatt gaaatattat caaggttaca ccctatctac tacaatggtt
    21001 tatgatcgtg gagaaggtga agaaactgag aaattagaag ataaacaaat tcaattagac
    21061 cttaaaaaag ttgaaatcaa aaacattaaa gaaacaagct taatgaatgt tgatgctgag
    21121 ggtaacgaaa ctgataagag cttattatct gaaaaaccga cagatgtttc ccaattgtat
    21181 ttacgtgtga caactcatga caacaaagtg acaagacttg cggtaagttc agttgaagaa
    21241 gttgttgttg atggaaaaac attgtacaaa gttgtagcca aagctccaga cttagttcaa
    21301 cgtagagctg atgatacact gagtgaagaa tatgttcatt attttgaaaa acaattacca
    21361 aaagtaaata atgtatacta caacttcaat gaacttgtaa aagatatgca agctaatcca
    21421 acgggtgagt ttaaacttgg tgcagacttg aatgcagcaa atgtaaaacc taacgggaaa
    21481 tcttatgtaa cgaaaccatt taaagggaaa ttactaagta atgacggcgg tagatttaca
    21541 atccataata tagaacgtcc attattcgct aatattgaag gtggtaaaat ccacgatatt
    21601 aatcttgcta atgtaaatat taatatgcct tgggctgata aagttgctcc tattgcaaat
    21661 gtaatcaaaa ataatgcaac cattgaaaat gtcaaagtta ctggtaatgt cctgggtaaa
    21721 gactgggtat ctggatttat agacaaaatt gacggcagtg gtaaattaat taatgttgca
    21781 tttatcggta atgttacatc tgttggtact ggaggcaact tcttaacagg tattgtggga
    21841 gaaaactgga aaggatacgt tgaaagagca tatgttgatg ctcatattaa aggtaagcga
    21901 gctaaagctg cgggtatagc ctactggtca caaaaccaag ggaataactt tactattgga
    21961 tctgaggggg ctattaaaaa atctgttgtt aaaggtacaa tagacgttga aaaaccaatt
    22021 gaagttggtg gcgctgttgg aagttttact tatcatggtt ctattgaaga tactgtttct
    22081 atgatgaaag ttaagaacgg tgaaattttc tatggttcta aagatattga tgatgatcca
    22141 tattacacag gtaaccatgt taatcgaaac tacgttgtta taggtgttag cgaaggtact
    22201 tctacatata gatattctaa tcagcataat cgtattaaac ctataacaca atcagaagct
    22261 gatgttaaaa ttgcagagac agctatcaca gcagataaat ttacaattac cgatccaatt
    22321 gtaaataaac ttaacgctct cacaacacgt gataatgagt atagaacaac acaagattac
    22381 gaggcgactc gcgaacaagc ttatcgcaat attgaaaaac ttcaaccgct ctataacaaa
    22441 gaatggattg tcaaccaagg taacaaactt gtagaaggtt caaacttgtt gacaaaagaa
    22501 gtattatctg ttacaggtat aaagagcggt caattcgtaa ctgatttgtc agatattgat
    22561 aaaattatga ttcactattc tgatggagca aaggaagaat tgaatgttac tcgtcaagaa
    22621 tcaaacgttc aacaagtgcg tgagtacagt attacagatt tagatatact ctatacacca
    22681 aatatggttg agaaagatcg cgctcagttg atgactgatg taaagtctaa gctatctagt
    22741 gtagaattag agtctgatgg agtgcgtcaa cttctcgtta agagagatac taagaaagat
    22801 gcgaatgcaa atagtgtagg tcgtcaaaat ggatacatta gagatttatt ccttgaagaa
    22861 agtttctctg aagttaaagc aaatcttgac aaattggtaa aacagatact tgaaaatgaa
    22921 gatcatcaac tcaacgataa cgaattagct gaacgtgctt tactgaagaa agttgaagat
    22981 aataaagcta aaatcatgat gggactggca tacttaaatc aatattatgc ttttaaatac
    23041 gatgagttat caattaaaga tattatgatg tttaaacctg atttttatgg taagacagca
    23101 agtgttattg atagattgat taatattggt tctgcagaaa ataatctaaa aggagataga
    23161 acgcaagatg cttatcgtgg tataatttca ggggcaacag gtaaaggtag tttacatgac
    23221 ttcttgacct acaatatgaa attattcaca aatgaaacag acataaatgt ttggtttaag
    23281 aaagctattg aaaagaatgc ttatgtagtt gaacaaccat caacgaactc tgcatttgcc
    23341 aacaaaaaat atcgtctata cgaaggaatt aataatgggc aacatgggcg catgatttta
    23401 cctcttctta acttgaaaaa tgcacatcta tttatgattt caacatacaa cactatctcg
    23461 tttagttcgt ttgaaaaata tggtaaagac acagatgaaa aacgtgaaaa gttcaagagt
    23521 gagatcaata aacgtgctaa agagcaggtt aattacttag atttttggtc acgtcttgct
    23581 actgataatg tccgtgataa acttcttaag agtcaaaatg tagttccaac tcctgtatgg
    23641 gataatcata atagcccgaa cggatgggct agtagacatg gtcatataga tgggaaacca
    23701 gattatgctc caattcgtga gttctttggg cgaatcaata agtatcatgg ttacaaatat
    23761 ggatatggcg catatgcata tatttttgca gcacctcaac cgatggatgc agtttatttc
    23821 gttatgacag atttaattag tgactttgga acatctgcat ttacacatga aactactcac
    23881 gttaatgatc gtatggctta ctatggtggt cattggcatc gtgaaggtac ggatcttgaa
    23941 gcatttgctc aggggatgtt acaaacgcca tcggtatcaa atccaaacgg agaatatggc
    24001 gcattaggtc taaatatggc gtatgaacgt caaaatgatg gaaatcaatg gtataacaca
    24061 aatccaaacg accttacaac tcgtgcagaa attgataact acatgaaagg tttcaacgac
    24121 acattaatgc ttcttgatta ccttgaagga gaagctgtcc tcaataaagc aaatcaagat
    24181 ttaaacaacg cttggttcaa aaaagtggat aagcaacttc gtggagctag tacgaagaac
    24241 cagtatgata aagttcgtga tttaacggca gatgagaaaa atatcactct aaattcagta
    24301 gatgatttag ttgataataa ttttatgacg aaacacggac ctggaaacaa tgtatatgat
    24361 ccaactgggt tcggaacggc ctacgtgact gtacctatta ctgcaggtat ttatggaggt
    24421 aacacaagtg aaggtgctcc tggttctatg tcattcaaac ataacacatt cagaatgtgg
    24481 gggtactacg gatatgaaaa aggattctta aactatgctt ctaacatgct gaaaaatgag
    24541 tctagacagg ctggtcacaa tactctaggt gatgatttca tcattaagaa agtttctgat
    24601 aataaattca gtacattgga agattggaag aaagcatact tcaaagaagt tgtagataaa
    24661 gctaaagcag gatttaaccc tgttacaatc gacagtacta catatagtag ttacgatgac
    24721 ttgaagaacg catttgctgc agcagttgaa aaagataaag ctactcttaa aaatggatca
    24781 gttaaatctg acaatactgt tgcacttaaa gagaaaatat acaaaaaact tcttcaacaa
    24841 acagatagct ttaaaacttc aatctttaga taatcgaaat ctctcatctg ctttgcggat
    24901 gagggttttc ttattttatg ctatacttaa agtatgcata gaaaaacagt gattgatttt
    24961 aggactttgg gggagagata tacctttacc cagcctatta aagagttgaa aacgagaaat
    25021 gtagaagaag tggcagattt gctggcacaa gtggaaaact atcaaacgca gggctactat
    25081 gtggtggggt atgtcagcta cgaggctgca cctgcttttg aggagaaatt agcagttcac
    25141 aaggttcctc tactgggcga gtacttgctt tactttactg ttcacgatag ggtggagacc
    25201 tcccctattc ctctgactta tgaggatatt gatttgccct caaattggca ggaagtaacg
    25261 tctgcagagg actatgaaaa ggccattgcc cagatacacc atcacttgag gcagggagac
    25321 acctatcagg tcaactacac cgtccaactc aagcaaaagt taagtgccaa tccttttgcc
    25381 atctacaatc gtatggtggt agagcaggag gcgggctaca atgcctatgt ggaacatgac
    25441 gagatggcag tgatttccat gagcccagag ctcttttttg agcaaaatga tcgcgagttg
    25501 acaacacgac caatgaaggg gacgactcag cgtggggtaa ctgaccaaga agatcttgaa
    25561 caggccagtt ggttggaaca ggatcccaaa aatcgctctg aaaatatgat gattgtggac
    25621 ctcttgcgca atgatatgaa ccgtatttct gaagttggga gcgagcacgt ggagcgtctg
    25681 tgtcaggtag agcagtattc aactgtttgg cagatgactt cgaccatcaa gagtcagttg
    25741 cgagaggatg tggaccttgt tgaaatcttc cgctcactct ttccttgtgg ttccataacg
    25801 ggtgcaccga aaattgcgac aatggagatt atcaaggact tggagcctca accgcgtgga
    25861 gtctactgtg gaacgattgg tctcttgctt ccaaatggac gacggatttt taatgtggcc
    25921 attcgtacca ttcaacttca caaaggtcaa gccatttatg gagttggcgg agggattact
    25981 tgggatagca catgggaatc tgaataccga gaggttcatc aaaaggcagc agttctttat
    26041 cgtaaacaag ctcgcttcca actgattacg acaggggaaa tcagccagaa gaacctgctg
    26101 tttgaagatc aacatctgga aagactgaga aaagctagtc gttattttgc ttttcctttt
    26161 gatgcagaag acttgggaca caagattgag gaagagtgtc aggattgtga agctaatcga
    26221 gattaccgct tgcgaatcag ccttagcaaa tctggagaga tagaagtcaa tcgtcaagta
    26281 ttaacccctc tcagtacaag cttttgtcag gcccaagtct gtcttcagga agctgctttg
    26341 aatcaatcct ttacctactt taaaaccact caccgatcgc atttgagcct aggagaacaa
    26401 gagaagattt accacaataa gtcaggagaa ctgcttgaaa cctctatagg aaatttggtt
    26461 ctgaaaatcg ctggaaaact ctacacaccg cctatccgac ttggaatctt gccaggaatt
    26521 taccgtcagc atttgctaga aacaggacag gtagaagaga aagtcttgac cttggcagat
    26581 ttaacccaag cagaagctat ttacggctgt aatgcagtga gaggcttgta tgaactaagc
    26641 cttgaggaga actagatgaa attgatattt ttgcatgggt tagggcagtc agcagagtct
    26701 tggaaagaag ttcggaatct actgacagat tatccttctg aggctatcga gttatttcct
    26761 tctggagtta gtaactatca acaagctaaa gagcgagttt atcagcacct agctcaagag
    26821 acagaacctt ttgttttgat tggattgtcc ttaggtgctg ccctgacact agaactttcc
    26881 agttacgatt taccaaatct tcgagctttg attctgtcag gatgcccgct taaattagca
    26941 gggaatatcc tattttatct tcagttgctg atttttaaac tacttcccaa aagagtattt
    27001 gaaaaacagg gagcagacaa gactcttatg gtcggagttt ctgaggaatt gaagacactt
    27061 gatttaacgg atatagcagg gacttgctcc tatccaacct tactaatttg tggcagcaaa
    27121 gataaaccga atcttagttc tatgaaagct ctccatagac tgttaacaga ttcgcagttt
    27181 cagatcattc ctgatggccc tcatgtcttg aataaggcaa aaccaaagga gtttgtagaa
    27241 aagaccagaa gtttccttga attgctgaaa taagtttgat aaaataatat tgaaatcgat
    27301 atcataatta tgggagaaag aaatgaataa acgtctattt ttaaaaatga gtctggtgac
    27361 gttgccaatt ttagccttgt tttcacaacc agttttggcg gaagaaaaca tccatttttc
    27421 gagctgtaag gaagcttggg cgaatggcta ttcggatatt cacgagggag aacctggtta
    27481 ttctgccaag ttagaccgtg atcatgatgg tgtggcttgc gaattgaaaa atgctcctaa
    27541 gggtgctttt aaagcaaaac agtcaacggc tattcaaatc aacacaagtt cagcaacaac
    27601 aagtggttgg gttaagcagg acggcgcttg gtactacttt gatggaaatg gaaatctagt
    27661 gaaaaatgca tggcagggaa gctattacct gaaagctgat ggtaaaatgg cacagagtga
    27721 atggatttat gactcttctt atcaagcttg gtattatttg aaatcagatg gttcttatgc
    27781 aaaaaatgca tggcaaggag cttattacct taaatcaaac ggtaaaatgg cacaaggtga
    27841 gtgggtttat gattcttctt accaagcatg gtattacttg aaatcagatg gttcatatgc
    27901 tcgcaatgca tggcaaggaa actactattt gaaatcagat ggtaaaatgg ctaaaggtga
    27961 atgggtttat gatgccacct atcaagcttg gtattatttg acatcagatg gttcttatgc
    28021 ttacagtaca tggcaaggaa attactatct aaaatcggat ggtaaaatgg ctgtcaatga
    28081 atgggttgat ggtggacgtt attatgttgg cgctgacgga gtttggaagg aaggtcaagc
    28141 aagtacagtt tcttctagta atgatagcaa tagtgaatat tctgctgctt taggaaaggc
    28201 aaaaagttat aattcgttat tccacatgtc aaaaaaacgt atgtatagac aattaacttc
    28261 tgattttgat aaattttcaa atgatgcagc tcaatatgcc attgatcatt tagatgatta
    28321 acagatactg gtaggcgaaa aaattcctta taattcattt acgcaaacct ttgcataagt
    28381 gaatggattt tgatatacta gtactgtaag cattttcaat tactacacaa tgaaaaagga
    28441 gaatatgatg agtcaaaaga ttattgggat tgaccttggt ggaacttcta tcaaatttgc
    28501 aatcttaaca acagcaggag aaatccaagg aaaatggtca atcaagacca acattttgga
    28561 tgagggaagt catatcgttg atgatatgat tgagtctatt caacatcgtt tggacttgct
    28621 tggattggca gcagcggact tccaaggcat tggaatggga tcaccaggtg ttgttgaccg
    28681 tgaaaaaggg actgttatcg gtgcctacaa cttgaactgg aaaacccttc aaccaatcaa
    28741 acaaaagatt gaaaaagctt tgggcattcc atttttcatc gataatgatg ccaacgtagc
    28801 agctcttggt gagcgctgga tgggtgctgg agataaccaa ccagacgttg tctttatgac
    28861 actcggtact ggtgttggtg gcggtatcgt cgcagaaggc aaattgcttc acggtgttgc
    28921 tggtgcagca ggtgagcttg gtcacatcac tgttgacttt gaccagccaa tctcatgtac
    28981 ttgtggtaaa aaaggctgcc ttgagacagt tgcttcagca acagggattg tcaacttgac
    29041 tcgtcgctat gccgatgaat acgaaggcga tgcagccttg aaacgcttga ttgataacgg
    29101 agaagaagta actgctaaga ctgtctttga tctcgcaaaa gaaggagacg accttgcttt
    29161 gattgtttac cgtaacttct cacgttactt gggaatcgct tgtgctaaca ttggttcaat
    29221 cctaaaccca tcaacaatcg tcatcggtgg tggtgtgtca gctgcgggag aattccttct
    29281 acaaggtgtt caaaaagttt acgatgaaaa tagtttccca caagtacgca catctactaa
    29341 attagctctt gcaactctag gaaatgacgc tggagttatc ggagcagcat cacttgtatt
    29401 gcaataagat aatgagagag gaaaatgcta acctagagtt agtaatgttc ctcttttatt
    29461 ttttatgcta tactagttag gacaatgaat gaggtgaaac gaaatgacaa aagcagatac
    29521 gatttttaaa gagaatattg aatgcattct aaaggaaggt gttttctcag agcaggctcg
    29581 tcctaagtac aaggatggga ctgttgctaa ttccaagtat gtaactggtg ccttttccga
    29641 gtacgactta tctaaaggag aatttcctat cacaaccttg cgtcccattg caatcaaatc
    29701 cgccatcaaa gaagttctct ggatttacca agaccagtca aatagcttag aagtgcttaa
    29761 tgacaagtac aatgttcact actggaatga ctgggaagtt ggagacacgg gaaccattgg
    29821 tgagcgctat ggtgccgttg ttaagaaaca cgacattatc aataagcttc tcaaacagtt
    29881 ggaaaccaat ccttggaacc gccgcaatat tatttcgctc tgggattacc aagctttcga
    29941 agaaacagat gggctgctcc cgtgcgcctt tcagaccatg tttgatgtcc ggcgtgttga
    30001 tggggaaatc tatctggatg cgaccttgac ccagcgctcc aatgatatgc tggtggccca
    30061 ccacatcaac gctatgcagt atgtggcttt acagatgatg attgccaaac attttggttg
    30121 gaaggttggg aagttcttct atttcatcaa caacctccat atctatgata atcaatttga
    30181 acaagctcag gaattgctcc gtcgggagcc gtcaaactgc caaccacgct tggttttaaa
    30241 tgttcctgat gggactaatt tctttgatat caaagcagaa gattttgagt tggtggatta
    30301 tgaccctgtt aagccacagt tgaagtttga cctagctatt taaaagaata gaaaaaagaa
    30361 gttgagaata atcccaactt cttttgtttc ttaacgtgat acgcggcgac gagctgcttt
    30421 tttacgattt tcttcgatga aagctgcttt ttgctcttct ggttcgatta ctttcttttt
    30481 aaatgcgtat actgcacctg caacggcagc gacagttcct gcgacacctg ttacaagacc
    30541 tttagcgaat cctttagcca tgagtcttcc tcctttatat tctcaatcag ccagcctcct
    30601 caagaggtca catttttctg actgaccttt ttgtgttata ataatagtaa cgaaaaaatg
    30661 ggaatttttc aaggaaaaaa gatgaaaaca aaaataattg tgattgttgg accgactgct
    30721 gttggaaaga cggctctagc tattgaagtt gcaaagcgct ttaatggcga agtggttagt
    30781 ggagatagcc agcaagtcta tcgaggactt gatattggga cggccaaggc tagtccagaa
    30841 gagcaggcag ctgttcctca tcatttaatc gatgttagag agataaccga gtcttactcg
    30901 gcttttgatt ttgtttcaga agctaagatg actattgagg atattcagag ccgtggcaag
    30961 ctagccatta tcgccggtgg gactggactt tatatccaga gcttgctaga aggttaccac
    31021 ctaggtgggg agactcctca tgaggagatt ttagcttatc gagctagttt ggagccatat
    31081 tcagatgagg aattagccca tttggtggag caagcaggcc ttgagattcc ccagtttaat
    31141 cgtcgtcgtg ctatgcgtgc cttagaaatt gcccattttg gtcaggattt ggaaaatcaa
    31201 gagaccttgt atgaaccgct gattatctgc ttggatgatg aacgtagtca actttatgag
    31261 cgtatcaacc accgagtgga tttgatgttt gaggctgggc ttttggatga ggccaagtgg
    31321 ctgtttgacc attcccctaa tgtacaggct gctaaaggaa ttggctataa ggaactcttt
    31381 ccttatttcc gtggagagca gacctttgag gaagctagtg agagtcttaa acaggcgacc
    31441 cgtcgttttg ccaagcgtca gctgacctgg ttccgtaatc gcatgcaggt caccttttat
    31501 cagattggag aatctggtgt acaagaccgc attttaagcc agatagagga gtttttagat
    31561 gattgaaacg gagaaaaaag aggagcgagt cctgctgatt ggtgtggaat tgcagggtat
    31621 ggacagtttt gacctctcca tggaagaatt ggctagttta gcgaaaacgg caggggcagt
    31681 cgttgtagat agctacagac aaaaacgtga aaaatatgat tccaagacct tcgtcggctc
    31741 tggtaagttg gaagagattg cgcttatggt ggatgcagaa gaaatcacta ctgtcatcgt
    31801 caacaatcgt ctgaccccaa ggcagaatgt caatctagag gaagttctcg gtgttaaggt
    31861 cattgaccgt atgcagttga ttttggatat ctttgccatg cgggctcgaa gccatgaagg
    31921 gaagctccaa gtccacctag cccaactcaa ataccttttg cctcgcttgg ttggtcaggg
    31981 gattatgctc agccgtcagg cagggggaat tggttcccgt ggtcctggtg aaagccagct
    32041 ggagctgaac cgtcgtagcg ttcgcaatca aatcacggat atcgagcgcc agctcaaggt
    32101 ggttgagaaa aatcgggcga ctgtcagaga aaaacgtttg gagtctagca cctttaagat
    32161 tggtttgatt ggttatacca atgctgggaa atcaactatc atgaacatct tgaccagtaa
    32221 gacccagtat gaagcagatg agctctttgc gactctggat gcgacaacca agagtattca
    32281 tctgggaggc aatctccaag taactttgac agataccgtt ggctttattc aagatttgcc
    32341 gacagagttg gtgtccagtt tcaagtcaac cttggaagaa agcaagcatg tggaccttct
    32401 ggttcatgtt atcgatgcta gcaatcctta ccacgaggag catgaaaaaa cggttctctc
    32461 catcatgaaa gacctggaca tggaagatat tcctcacttg acgctttata ataaagcgga
    32521 tttggtggag gatttcacgc ctacccaaac gccatatacc ctcatttctg ccaagtctga
    32581 ggacagtcgt gaaaacttgc aagcattatt gctagataag attaaggaaa tttttgaagc
    32641 atttaccctg cgagtgcctt tttcaaagtc ctacaagatt catgatttag agagtgttgc
    32701 aattctggaa gaacgtgatt atcaggaaga cggcgaagtg attacaggct acatttccga
    32761 gaaaaataaa tggaggttag aagaatttta tgactgatat taaaactttg gccctaaagt
    32821 atggaggtta tacaagtctg gacaaggtct atctggatca gcttctagct ggtaaaacag
    32881 agcaggagca gttaacactt attacacctc cgccaagtgt tgttaatgcc tactttgcag
    32941 aactctatca gaaaaaaagt cctcaggctg cgacggatta ttttgcagaa ctcagtcagg
    33001 aactgaatct ttacaatact gagccaagtt tcaccctaga aaataagcct tttattcggc
    33061 ttaatctgtc tggtaaatcc tttggttttt gttatgagag tgacggcttt ggtcggattt
    33121 tctctgaaaa tgaggagaaa atctcggatg acttgctctt tgaaattgcg caaattttct
    33181 cccatcaatt agtctttgaa gagtctggta agatttacat gaagcctgtc aaggacgagg
    33241 aagttgtcag cctagaaagt ctcacagctt tgactgattt ggaaagattg gctgatggtc
    33301 gaaaacgtct caaaggctac agccaagagg atttattgca agaagctgct gctttttctg
    33361 gcaaacgcta tttccgatcg gaaaaccgca cagccatgtt atatattgat taattagaaa
    33421 gtatcgaatg gatattcaat ttttaggaac gggggctggt cagccctcta aagcccgcaa
    33481 cgtttcaagt ctcgccctga aacttttgga cgagattaac gaagtttggc tctttgactg
    33541 tggagaaggt acgcaaaatc gcattctgga aaccacaatt cgaccacgta aggtcagcaa
    33601 aatctttatt acccatctgc atggagacca catttttggt ttgccaggtt tcctttctag
    33661 ccgtgccttt caggccaatg aagagcagac agatttggaa atctacggac ctcaaggaat
    33721 caagtcattt gtcttaacca gccttcgtgt gtcaggttct cgtctgccct accgcattca
    33781 tttccatgag tttgaccaag attctctggg taaaattctt gaaaccgata aattcactgt
    33841 gtatgcagag gagctggacc acactatttt ctgtgttggc tatcgtgtca tgcaaaagga
    33901 tctagaaggg acgctggatg ctgaaaaact caaggctgct ggtgttccgt tcggcccgct
    33961 ttttggtaaa atcaaaaacg gccaggatct tgttttggaa gacggaactg aaatcaaggc
    34021 agcagactat atctcagcgc cacgtccagg taagattatc actattttag gagacactcg
    34081 aaaaacggat gccagtgtgc gtctggctgt caatgcagat gtcctagttc atgagtccac
    34141 ttatggcaag ggtgatgaaa aaattgctcg taaccatggt cactcaacta atatgcaagc
    34201 tgcacaagta gcggtagaag caggtgccaa acgcctccta ctcaaccata tcagtgcccg
    34261 tttcctctca aaagatataa gcaaactcaa gaaggacgct gccacaattt ttgaaaatgt
    34321 ccatgtggtc aaagacttgg aagaagtgga aatctagcag tcacagaaag gataagtatg
    34381 cctactattc tcattaccgg agctagcggt ggtctagctc aagaaatggt caaactcctg
    34441 cccaatgacc aactcatctt gcttggtaga aataaggaaa aattagccca actctacgga
    34501 aattattccc atgcagaatt gattgaaatt gatattaccg acgattcagc cctagaagct
    34561 ctggtaactg atctttatct ccgctatggc aagattgatg tcttgattaa caacgctggt
    34621 tacgggattt ttgagggatt tgaccagatt gctgataaag atattcacca gatgtttgag
    34681 gtcaatacct ttgccctgat gaatctgtct cgtcaccttg cggctcgtat gaaggaaagc
    34741 agcaaagggc atatcatcaa catcgtcagc atggcaggtc taatagctac tggcaagtct
    34801 agtctttact cagcgaccaa gtttgcggct attggttttt caaatgctct gcgactcgaa
    34861 cttatgccct atggagtcta tgtgacaaca gtcaatccag gtccaatccg aacaggattt
    34921 tttgaccaag ctgacccaga tggaacttat cttaaatcgg ttgaccgctt cctcttagag
    34981 gcagatgcag tggctaaaaa gattgtcaag attataggca aaaataaacg agaactcaat
    35041 ctcccgattt tgttgaacct agcccataag ttttatactc tctttcccaa gctagctgat
    35101 aagttggcag gggaaacttt taattataag taaaaagaac caatgtgcag gttgttgcta
    35161 gcctacatat tggttcttat catttctcaa ctatcaaact gaacttcttc tagtagatag
    35221 tcgataaagc gttcgcccat cttagatagg ctggttttct catgctggat atagaccagc
    35281 tcaatcgggt catcaatatc cagtgggata gaaacgatat tgtctccgtt taggttgctg
    35341 ttcaaaatcc ctgtcgcaat ggtataacca tccaaaccaa tcaagagatt aaagagggtg
    35401 gcacggtcac tgaccacaat ggatttcttg tggtgttctt gagaaagaat ctcttctgaa
    35461 aagtagaagg agttgtgcgt cccttggtca tagctgaggt aagggaaatt ctccaaatca
    35521 gacagtttca ccttgtcttt ctttgccaga gggttggtct tgctgacaaa gatatgcggt
    35581 tgcgctgtga agagatggtg ggctagcagg tgattgtcat ccagcatctt ggttaaaaca
    35641 tcacggttgt aactgtttaa gaagaggacc ccgacctcac tgcggaagtt cttgacgtcg
    35701 tcgataatct cccaagtccg agtttcacga aggaagagtt cgtatttctc catatcgctt
    35761 ttcttgagca aagagacaaa ggcattgacc acaaaggcat agtgttgaga cgaaacgcta
    35821 aagagttcgc ggtgggcgac aggattttta tagcgttcct ccagaagctg ggtctgctcg
    35881 acaacctgac gggcataaga gagaaactcc atcccatcac gggtcaaggt gattcccttg
    35941 ggattgcgga taaagatctc aatgcccatt tcattttcca aatctctcac agcattggaa
    36001 agacttggct gagtgataaa gagttgcttg gctgcctcat tcatggagcc agtttcgacg
    36061 attttgataa tataatgtaa ttgttgaatt ctcatgtttt tattgtacca cacttgcctt
    36121 agaatcgcta aagaagatag gaaaataagg agcattgtgt gagtctcttc ttgaaaaaaa
    36181 cacaaaattt tgctagaatg agtcttatga aaacattcta tgatgtgcaa caattcctca
    36241 aacgatttgg tattattgtt tacatgggaa aacgcttata tgatattgaa ctgatgaagt
    36301 tggaactatc tcggatttac gatgcagggt tgatggataa attagactat ctagaggcag
    36361 aagcagttct tcgcagagag cacaaggtag aattggatta tattgagaaa aatggagaaa
    36421 agaactaatg gttacttgga ttttgtgggc acttatacta gcaatgttgg cgtggatggg
    36481 ctttaactat cttcgtattc gccgtgcggc taaaattgtg gacaatgagg agtttgaagc
    36541 cttgattcgt acgggtcaat tgattgattt gcgcgaccca gcagaattcc acagaaaaca
    36601 tatccttcgt gcacgcaata ttccttcaag tcagttgaaa actagtcttg cagcccttcg
    36661 taaagataaa cctgtccttc tctacgagaa ccaacgtgcg caacgagtta caaatgcagc
    36721 tctttacttg aaaaaacaag gtttttctga gatttatatc ctttcttatg gcttggattc
    36781 ttggaaaggg aaagtgaaga ctagctaacc ttatttccaa attagtcgaa tgataggatg
    36841 aagctattgt tttatagtat aattaatttt tataaaattt aaagagttct tagaacatga
    36901 aagaaaagaa gaaaaatcca ctttttgtgg gtatcttgtc gattattctt ggtttgttat
    36961 ttccaattgt aggcctgatt ttgggtatca taggattggt cttggctatt tcataccaaa
    37021 aagagtcaca attagactat aaaatagaaa agattcttaa tatcttagga attgtgattt
    37081 ctgtagttaa ctggattgta gcaatcgcct taatttttcg ataaataaaa gaaaaaagct
    37141 ttagttttaa agcttttttt gttttatcga ctcatctcta aatagttttt aatgatttgt
    37201 aactcttttt ggttcaaagg gcggtagtta ccttctgcta aatctgggtt caatgtgaag
    37261 tccccaaatt ggattctctt gaggctagtc accttaacac caaccgagag gaacattttc
    37321 ttgatttgat gaaattttcc ttctgaaatg gtgatagagg cttgactgat ggagggactt
    37381 gcagatagaa tctctagttt tgcgggttta cagacagtac catctaaaaa gacaattccc
    37441 ttttgaaagg cttggatatg gtcaggtgtt agaagtccat taacctcaac ttgataagtc
    37501 ttatcgacat gatattgggg atggaggagt tggaagccca agggaccgtt atcggtcaag
    37561 agaaggagtc ctgttgtatc tcggtccagt cggccgacgg catagagctt gtcagactgg
    37621 atgtcagatg gaagcaggtc catgacggtc ggaagttcct tgtctttgtt ggctgtaacg
    37681 acaccagcag gtttatgaag cataagatag gtgtgttcat agccttgaat gattcgatcc
    37741 tgaaaaagga gttcttgtag tcctgtatcg atattttggg ctagggagcg ggctgggcaa
    37801 ctatcgacga gaatgtcccc tttgaataag gcttgtttca tggcctttcg gctgattttt
    37861 tcttgagcta ataaattatc taaacgcata ctgtgcctat cttatcataa gtcgcttgct
    37921 ttgaaaaggc ttgacgtgaa aagaaaagcg gagtgaaaac atttacactt gcttgagtta
    37981 tgttatttat ttgaaattat ggtataatcg ttcagttaga aaataaattt tgaatattat
    38041 agaggaaatc atgacaaaat taagagaaga tatccgtaac attgcgatta tcgcccacgt
    38101 tgaccacggt aaaacaaccc tcgttgacga attattgaaa caatcagaaa cgcttgatgc
    38161 acgtactgaa ttggcagagc gtgctatgga ctcaaacgat atcgaaaaag agcgtggaat
    38221 caccatcctt gctaaaaata ctgccgttgc ttacaacgga actcgtatca acattatgga
    38281 cacaccagga cacgcggact tcggtggaga agttgagcgt atcatgaaaa tggttgacgg
    38341 tgttgtcttg gtcgtagatg cctatgaagg aaccatgcca caaactcgtt tcgtattgaa
    38401 aaaagccttg gaacaagacc ttgtcccaat cgtggttgtt aacaaaatcg ataagccatc
    38461 agctcgtcca gcagaagtag tggatgaagt cttggaactt ttcatcgagc ttggtgcaga
    38521 tgacgaccag cttgatttcc cagtggttta tgcttcagcg atcaacggaa cttcttcatt
    38581 gtcagatgat ccagctgacc aagaagcgac tatggcacca atctttgaca cgattatcga
    38641 ccatatccca gctccagtag ataactcaga tgagcctttg cagttccaag tgtcactttt
    38701 ggactacaat gacttcgttg gacgtatcgg tatcggtcgt gtcttccgtg gtacagttaa
    38761 ggttggggac caagttaccc tttctaaact tgacggtaca actaaaaact tccgtgttac
    38821 aaaactcttc ggtttctttg gtttggaacg tcgtgaaatc caagaagcca aagcgggtga
    38881 cttgattgcc gtttcaggta tggaagacat ctttgtcggt gaaaccatca ctccgataga
    38941 tgcagtagaa gctcttccaa tcctacacat cgatgagcca actcttcaaa tgactttctt
    39001 ggtcaacaac tcaccatttg ctggtaaaga aggtaaatgg gtaacttctc gtaaggtgga
    39061 agaacgcttg caggcagaat tgcaaacaga cgtttccctt cgtgttgacc caactgattc
    39121 accagataaa tggactgttt caggacgtgg agaattgcac ttgtcaatcc ttatcgaaac
    39181 aatgcgtcgt gagggctatg aacttcaagt atctcgtcca gaagttatcg taaaagaaat
    39241 cgacggtgtt aaatgtgaac catttgaacg tgtacaaatc gacactccag aagaatacca
    39301 agggtctgtt atccaaagcc tttctgaacg taaaggtgaa atgttggata tgatttcaac
    39361 tggtaatggt caaactcgtt tggtcttcct tgttccagcg cgtggtttga ttggatactc
    39421 aactgagttc ttgtcaatga ctcgtggtta cggtatcatg aaccatacct tcgaccaata
    39481 cttgccattg attccagggg aaattggtgg acgtcaccgt ggtgcccttg tttctatcga
    39541 tgctggtaag gctacaactt actcaatcat gtctatcgaa gaacgtggta cgatctttgt
    39601 caacccaggt actgaggttt atgaaggaat gatcatcggt gaaaactctc gtgaaaatga
    39661 cttgacagtt aacatcacga aggcaaaaca aatgaccaac gttcgttcag ctactaagga
    39721 ccaaacagct gttatcaaga cgcctcgtat cttgacactt gaagagtctc ttgagttctt
    39781 gaacgacgat gagtacatgg aagtaacgcc tgagtctatc cgtttgcgta aacaaatcct
    39841 taacaaagca gagcgtgaga aagctaacaa gaagaaaaaa tcagctgaat aagagctaga
    39901 aagagagaaa gatggtctat ttagtcctag gaattttact gctcctactc tatgtatttg
    39961 cgacaccaga aagcattaaa gggactgtca atatcgtcgc tatggtatgt attttagtgg
    40021 cactcttgat tttattggtt ctatcttttc tgaaaatttt tcaattacca acagaaatat
    40081 tcctagcaat agccatgttg atcctagctt actttagtgt tagagacatc acactcatgc
    40141 cagtcaaaaa aagtaaaaga agataaaaaa gagagtttcg gctctctttt tgctaggaat
    40201 tttacaatag tttgaattgc tttttagtta acaccatctc ttttacttgt atagtatgaa
    40261 tgggaaaaac ttgaataaaa ttcgttaatg gcatctattt tatatttgct tagtactggt
    40321 tgcatattat ttcgaaataa aactactata taatattatt cacagaggta atatagtaaa
    40381 atatctaaaa aattcgattt tttccttgac aacaaataat aaaaaagtta ttcttatcac
    40441 ataagaaagc gatttcaata aaagatagag gaggatttat tttgttgcat tgttgctcaa
    40501 ggtgtgcttt ttttgaaaga aaaaataaaa tgaaaacgaa aaaacataga ttacttgctc
    40561 tagctcttat ttcaaatttt acattattgg gagctgcatc agctgctgta caatatccag
    40621 atggaggagt atggacatat ggagaaggtt caggaggtgg ttaggctttt tcaaattact
    40681 atcatggtaa aaaatatcat tattcatcca tcgtgagcag atgggatggt cattctgata
    40741 aaggagaagc tcctgctgga aaaacctcct atgcatggat ttggactaaa tggggagaac
    40801 aagtagcatt ttactgtgat tatgactaat cgttcattga tttaaatcgt tccccttgta
    40861 agaaattaca gggggaattt tgtttacaag gagtctacta tgaagaaact attcatatta
    40921 ttatcaactt tttttctcag cttcttcctt gcttggatta ttgtcttacg tgcgccacaa
    40981 tatttatatg caagctatga ttccgtttct ttacttcgtg tcaaaaaaga tactcaggaa
    41041 ccgacgcgtg aggtatttga acaggaattg gagaattttg caaactcaga acagagttta
    41101 atagctagaa gaattgtaga gccgagtaag gatgggacga ctcactttac ttatgcaact
    41161 tatggtcagg gaactttacc aaaagaattc caagaagcta gccaagaaag tcgtgaacgt
    41221 agtgatccgc taaatagtta tctccttttg tcaggctcct tgacgaaaga aaagcttgcc
    41281 gataaattag gagatttggg ttataaagca agtgctgacc gaaagatacc gccctatttt
    41341 cttgcttttc gaatattact aaatcccctt attttaatta gtttagcaat atttggttta
    41401 tctttctttg ctttagtgat tatcactcgg attaaggaaa tgagagcagc aggtataaaa
    41461 ctcttttctg gtcagactct cttatccatc atggggcatt ctttatctac tgatatcaaa
    41521 tggctccttc tatcagccct cctttccttc ctaggtgggg gagtcgttct ttttagtcaa
    41581 ggtttgtttt atcctatctt gttagccacc tatggttttg ggattagttt ctatctgttg
    41641 tttttattgg cgatttcaat tttactaatg cttctttatc taatgagttt gaattacaaa
    41701 gcattagttc ccgttattaa ggggagatta ccccttaaac gcctgatgat tttaacccta
    41761 ttgtgtcagt tggttgctgt ctttacagta ggatacgctg ttaagacagg tttgacgtct
    41821 taccaacgat tgaaagaact tgaaatttca aaacaagcat ggcaggatag agcagactat
    41881 tatcaaattt cttttggctt aggtgataga ggaaaagata cagaaaatca gagcaagtgg
    41941 tatgcctttg ccaaggaagc aatcgaagaa gaacaagctc tttatgtaaa ggataatctg
    42001 ctccattttg ccaatccaca aggaaaaaat gaacagggag agacactgga tacctatagt
    42061 ccagatgcta atacgctcta tgttagtccc agttatttgg acaaggaaaa ggtcgtggta
    42121 gatgctgaga ccaaacagaa gttagcccat ctccaaaaag gtgagtttat cctcttgctc
    42181 ccagaacatt tgcgctctcg agaagcagaa cttaagaaag tttttgaaga aagattgagt
    42241 tattatggaa aatctggtga ggaggcaagt gctcctttgg attatgagat gaaagcgcac
    42301 gttagttatc tttcaatggg agaaaagcgg tttgtttata atagcggtga gaatcccgta
    42361 tctactcagt atttgactga tccgatttta gtggtattca cgccgacttc tacaggtgat
    42421 agttttatat ccttatctag ttggtctatc aatgctggaa aacaactctt tatcaaagga
    42481 tatgagagtg ggctagaact cttgaagaaa gttggaattt atgagcaagt atcctatctt
    42541 aaagaaggaa gaagtgttta tttaactcgt tataatgaag ttcaaactga aacagcaact
    42601 ttaatcttag gagctattgt ggggatagct agttccttgt tactatttta ttctgtcaat
    42661 cttctatatt tcgagcaatt ccgccgagat atcttgatta aacgaatttc aggtttacga
    42721 ttttttgaaa cacatgctca gtatatggtt agtcaatttg ccagttttgt atttggtgct
    42781 agtctcttta ttttaagcag tcgagacttg gtgattggct tgctcacttt attagtcttt
    42841 ctagctagtg cagttctgac gctttaccgt caagcgcaga aagaatctcg tgtttctatg
    42901 acaattatga aaggaaaata ggatgattga actaaagaat atatctaaaa aatttggaag
    42961 ccgtcagcta ttttcagata caaatcttca ttttgaaggt gggaaaattt atgccttaat
    43021 cggtacaagt ggctgtggta agacaacact cttgaatatg attggacgat tagagccata
    43081 tgacaaaggg caaatcatct atgatggcac ttctcttaag gacatcaagc cttctatttt
    43141 ctttagagat tacttgggat acttatttca agattttggc ttaattgaaa gccaaaccgt
    43201 caaagagaat ctcaatctgg gtttagttgg taaaaagttg aaggaaaaag agaaaatctc
    43261 tttgatgaaa caagctctaa accgtgtaaa cctctcttat ttggatttga agcaacctat
    43321 atttgagtta tcaggaggag aagcacaacg tgttgcatta gcgaagataa ttttaaagga
    43381 tccacctttg atcctcgcag atgaaccaac cgcttcctta gaccccaaaa actctgagga
    43441 attactttcc atcctagaat ctttaaaaaa tccgaatcgg accattatta ttgcgaccca
    43501 caatcctctg atttgggagc aagtggatca ggtcattcga gttaccgatt tatcacatag
    43561 atgatatggt aagattcagc tagaagaaag agtcacaaac acactttgtg gcttttttat
    43621 ttccataaaa atggtaaaat agtaggagta gaaatggagt tcgagacatg aaagtaatag
    43681 atcaatttaa aaataagaaa gttcttgttt taggtttggc caagtctggt gaatctgcgg
    43741 ctcgtttgtt ggacaagtta ggtgccattg tgacagtaaa tgatgggaaa ccattcgagg
    43801 acaatccagc tgcgcaatgt ttgctggaag aagggatcaa ggtcattaca ggtggccatc
    43861 ctttggaact cttggatgaa gagttcgccc tcatggtaaa aaatccaggt atcccctaca
    43921 gcaatcccat gattgaaaag gctttggcca agggaattcc agtcttgact gaggtggaat
    43981 tggcttattt gatttcagaa gcaccgatta ttggtatcac aggatcgaac ggtaagacaa
    44041 ccacaacgac tatgattggg gaagttttga ctgctgctgg ccaacatggt cttttatcag
    44101 ggaatatcgg ctatcctgcc agtcaggttg ctcaaatagc gacagacaag aacacgcttg
    44161 ttatggaact ttcttctttc caactcatgg gtgttcaaga attccatcct gagattgcgg
    44221 ttattaccaa ccttatgcca actcatatcg attatcatgg attatttgag gaatatgtag
    44281 cagccaagtg gaatatccag aacaagatga cagcagctga tttccttgtc ttgaacttta
    44341 atcaagactt ggcaaaagac ttggcttcca agacagaagc cactgttgta ccattttcaa
    44401 cacttgaaaa ggttgatgga gcttatctgg aagatggtca actctacttc cgtggtgaag
    44461 tagtcatggc agcgaatgaa atcggtgttc caggtagcca caatgtggaa aatgcccttg
    44521 cgactattgc tgtagccaag cttcgtgatg tggacaatca aaccatcaag gaaactcttt
    44581 cagccttcgg tggtgtcaaa caccgtctcc agtttgtgga tgacatcaag ggtgttaaat
    44641 tctataacga cagtaaatca actaatatct tggctactca aaaagcctta tcaggatttg
    44701 acaacagcaa ggtcgtcttg attgcaggtg gtttggaccg tggcaatgag tttgacgaat
    44761 tggtgccaga cattactgga ctcaagaaga tggtcatcct gggtcaatct gcagaacgtg
    44821 tcaaacgggc agcagacaag gctggtgtcg cttatgtgga ggcgacagat attgcagatg
    44881 cgacccgcaa ggcctatgag cttgcgactc aaggagatgt ggttcttctt agtcctgcca
    44941 atgccagctg ggatatgtat gctaactttg aagtacgtgg cgacctcttt atcgacacag
    45001 tagcggagtt aaaagaataa tatgaaaaaa attgtcttta caggtggggg gacggttgga
    45061 cacgttaccc tcaatctttt gttaatgccc aagttcatcg aagatggttg ggaagtccac
    45121 tatatcgggg acaagcgtgg tatcgaacac caagaaatcc ttaagtcagg tttggatgtc
    45181 accttccatt ctattgcgac tggaaaattg cgtcgctatt tctcttggca aaatatgctg
    45241 gacgtcttca aagttggctg gggaatcgtc caatcgctct ttatcatgtt gcgacttcgt
    45301 ccacagaccc ttttttcaaa ggggggcttt gtctcagtac cgcctgttat cgctgcgcgt
    45361 gtgtcaggag tgcctgtctt tattcacgaa tctgacctgt ctatgggctt ggccaataaa
    45421 atcgcctata agtttgcgac taagatgtat tcaacctttg agcaagcttc gagtttgtct
    45481 aaggttgagc atgtgggagc agtgaccaag gtttcagatc aaaaaaatcc agaaccagat
    45541 gaattggtgg atattcaaag ccactttaat cataaattgc cgactgtatt gtttgttggt
    45601 ggttctgcag gtgctcgtgt ctttaaccaa ttggtgacag accataagaa agaactaaca
    45661 gagcgctaca atattatcaa tctaactgga gattctagcc tgaatgagtt gagccaaaat
    45721 ctttttcgtg ttgactatgt gaccgatctc tatcaaccct tgatggaatt ggctgatatt
    45781 gttgtgacac gtggtggtgc caatacgatt tttgagctct tggcgatagc aaaattgcat
    45841 gtcattgtgc cacttggtcg tgaagctagt cgtggtgacc agattgaaaa tgcagcttac
    45901 tttgttaaga aaggctatgc agaagacctt caagaaagcg atttgacctt ggatagtttg
    45961 gaagagaagc tttctcactt actaagtcac aaggaagatt accaagctaa gatgaaagct
    46021 tctaaggaat tgaaatctct agcagatttt tatcaattgt tgaaaaaaga tttatcataa
    46081 ggaaagtaaa tgtcaaaaga taagaaaaat gaggacaaag aaatcctcga agaattgaaa
    46141 gagttatcag aatggcagaa acgaaaccaa gaatatctaa aaaagaaggc tgaagaagaa
    46201 gcggctctag ctgaggagaa ggaaaaggaa agacaagctc ggatgggaga agaatctgag
    46261 aagtcagagg acaaacagga ccaggagagt gaaacagacc aggaagattc agaatcagct
    46321 aaggaagagt ctgaagaaaa agtagcatcc tcagaggctg acaaagagaa agaagagaaa
    46381 gaagaatcag agtctaaaga gaaggaggaa caggataaaa agcttgctaa aaaggctaca
    46441 aaggaaaaac cagccaaagc aaagattcct ggtatccata tcttgcgagc cttcacgatt
    46501 ttatttccaa gtctgctttt attgattgtc tctgcctact tgctcagtcc ttatgcgacc
    46561 atgaaagata ttcgtgttga gggaacggtg caaactacag ctgatgatat tcgacaggtt
    46621 tcaggcattc aggattcgga ttatacgatt aaccttctgc tagacaaggc aaaatatgaa
    46681 aagcagatta agtctaacta ttgggttgaa tcagctcaac ttgtctatca atttccaact
    46741 aagttcacta ttaaggtcaa ggaatatgat attgtggcct actatatttc tggtgaaaat
    46801 cattatccta ttctttccag tggtcagctt gagactagtt ctgtgagtct gaacagttta
    46861 ccagaaactt atttatcagt tctctttaat gatagtgaac aaatcaaggt ttttgtctca
    46921 gaacttgctc aaattagccc agaactcaag gcggctatcc aaaaggtgga attagcccca
    46981 agcaaggtga catccgattt aattcgattg accatgaatg attcggacga agtcttggtt
    47041 cctctatctg aaatgagtaa gaaactgcca tattacagta agattaagcc tcaattgtca
    47101 gaaccgagtg tggtcgacat ggaagctgga atctacagct acactgtggc ggataaatta
    47161 attatggagg ctgaggaaaa agccaaacaa gaggccaagg aagctgagaa aaaacaggaa
    47221 gaagaacaga aacgtttaga agaagaacag aaaaaacaag aggaagagag caatcgaaac
    47281 caaacgaccc agcgctcatc gcgtcgctag gtttaccttt tctcttatag ttctttagtg
    47341 accatgtttt tacttgacaa gtgctagtag aaataataga ataatagaaa acctttaaag
    47401 cagtccagag aggcagctaa ggttagacgg tgaaagggtg gagactaccc atttttcgtg
    47461 gaaccttgct gttggcaggt tccttttttc gtggcttctg ttggccagac tctctcacta
    47521 gtaaaggtaa aaggagaaac ctatgcgaga acatcgtcca atcattgctc ttgattttcc
    47581 tagttttgag gcggtcaagg aatttttagc tcttttccca gcagaagaaa gcctttatct
    47641 caaggtaggg atggagcttt attacgcagc ggggcctgag attgtgtcct acttaaaagg
    47701 tttgggtcat agtgtctttt tggatctcaa acttcatgac attcctaata cagtcaagtc
    47761 agccatgaag gtcttgtctc agcttggtgt cgatatgact aatgtccatg cggctggtgg
    47821 tgtagagatg atgaaggcgg cgcgtgaagg tcttgggagt caagccaaat tgatcgctgt
    47881 aacgcagctg acatcaacgt cagaagccca gatgcaggag tttcaaaata tccaaaccag
    47941 tctgcaagag tctgtgattc actatgccaa gaagacagct gaagctggct tggatggtgt
    48001 tgtttgctcg gctcaggaag tacaagtcat caagcaggct accaatccag attttatctg
    48061 tctgacacca gggattcgtc cagctggtgt tgcagttgga gatcaaaaac gagtcatgac
    48121 acctgctgat gcctatcaaa tcggcagtga ctatatcgta gtgggacgtc ccattaccca
    48181 agctgaggat cctgttgcag cttatcatgc catcaaggat gaatggacac aggactggaa
    48241 ttaaagaact agattagaaa aataaaagga gaataccatg acacttgcta aagatatcgc
    48301 tagccacctc ttgaaaatcc aagccgttta cctcaaacca gaggaaccct tcacttgggc
    48361 atctggtatc aagtcaccga tttacactga taatcgtgtg acactagcct atccagaaac
    48421 tcgtacccta attgaaaatg gttttgtgga agctatcaaa gaagcctttc ctgaagtaga
    48481 agtgattgca ggaactgcaa cagcagggat tccacacgga gccattattg ctgataagat
    48541 ggccttgcct tttgcctaca tccgtagcaa accaaaagac cacggagctg gtaatcaaat
    48601 cgaaggtcgc gtagctcaag gtcaaaaaat ggtagtggtt gaagacctta tttcaacggg
    48661 tggttcagtt cttgaagctg tagcagcagc caagcgagaa ggagcagatg tacttggagt
    48721 tgtagcgatt ttcagctacc aattgccaaa agcagataag aactttgcag atgctggtgt
    48781 taaacttgtg acgctttcaa actatagcga gcttatccat ctagcccaag aagaaggtta
    48841 catcacgcca gagggccttg atcttctaaa acgctttaaa gaagaccaag aaaattggca
    48901 agaaggttag gtcagtaaga taaagagaga cgaggctacc gagtctcttt taccatttta
    48961 tttaaaatat gacaatattt taaataaaat aattgacaac gctttctttt attgatataa
    49021 tgaaaatgta atatgtatca aattgatcaa tcaactatct tgtttatcac ttcttaagag
    49081 gacgatgtca tgaaaagaag aaaaatatat tccttttttg cgtagttaca attgcatcta
    49141 gtttgtttct ccctacagtt ttagctagac agattggaaa ttatgattta acgttgccgc
    49201 gttggggttc ggatacaact agtgagcttg agaaagaaaa cgcctctgct ggaattaata
    49261 atagtgacag cactggtggc ggtaaaaggt taaatacctc tattcgtagc gcctatagtg
    49321 ggtcagatat taccccggta tattcatttg ggtctggctc taggattgtc atgtactata
    49381 atggaggtgg tgacaattat attggttctg gtactagatt agctatggcg ccacaatttg
    49441 gaaatcatgt aagaattcat acttcaggtt cttggagtcc agattcttat taacttactt
    49501 gtcagagtaa gccttaaaga tggttgattg tgggtgtagc atgaaaaaag aatgctacac
    49561 cctattttta ttataaggag gagtaaggat ggaatttttc atttgtaatc ttgtacgagt
    49621 cgttcaatca cctcgatttt atatgtcttt atttttgacc cttctttgca tgagtttagg
    49681 aaatttcctt gctttcaatg gtatttataa aattgaaggt ttatcgattt tttttgccgc
    49741 ttcttctatt cgaggatttt caccgattag cctagtagct gcacttatct gtacactgcc
    49801 ctattctagt cagataatag aggatgctga gagtcatttt ctaacagcac aattgtgtcg
    49861 aatttctaaa aagaagtatc tggctattgt gggtagtact gcaattattt cttcttttct
    49921 agtctttttt ctcccctatt tattattatt aggaattaat cttttagtga ctccttatca
    49981 ggaaatttat attggagatt atagtggtgc cttaaaagaa ttatttgatt ccaatcagtt
    50041 gctctatagt cttgtgacga ctctctggta tggagtttgg ggcgctgtgt tctctatttt
    50101 tggactagct agtgctttgc tagtgaagaa aaaaatagga gctattttca tcccagttgc
    50161 ctatatgatg gttggtggta ttttttgggc tattttaggg ctatcttact tagaacctgt
    50221 gacaacatta gcttttggat atcagaaaga tatcagtctt tccttagtta gtgggcatct
    50281 tgcttttatt ttatttgtta gttgtttggt tgtttatggt acattttttc tacattcaga
    50341 ggactacgta taatgaaaca atttgttcaa ttttacaaaa aagatttttt atcagtattg
    50401 gtttatttta tattactgct atcctgtgtt ttatctagta cagtatattt attgcgctgt
    50461 cgccaatatt caatccatcc aaatgtatta gaatggatct tagttttact tcaagatatg
    50521 acgactggag tatattgctt tccgttcaca tatatattgt tcttttttta tttgatgaat
    50581 aactatttta ataggttgga gtgtcgcatt cgtctgaaat caattaagca ctttaccagt
    50641 tttagtttca aattagcagc tcttagtacg gggatttgga cggcgacttt atttttattg
    50701 atttttctga ttgcatttag taatggtttt agcttctctt tggagataaa ggaggttgat
    50761 tttttaagag aattttatgg tataagtatt gcaaacaatg ctagtttctt tataggattt
    50821 tttttctctt atatagcata ctatttcttt ttatccttac ttactattag cagtttttct
    50881 tggtttaaaa aatcaaacat gagcttagta tttctgttta cttttttatt tgtagaatcc
    50941 ttattctgga tttatcagtt ggacaatggg ataattggat tattgccaat ttttcagtat
    51001 atggtaaatt ccaatccgta tgcattgatt tattggctta cattactatc tatcataatt
    51061 ccattgactg tattttctgt tcatagaaac tggaggagag tgtaaaagtt ggaaatggga
    51121 aagttaagta gtcacatgtg gaggttgaat cagataatct ataccaagta cttttggggt
    51181 tatgttcttt tttggatatt gatttgttta ggattatggt attggttaga aggaaatgat
    51241 agacttgtta tagaaatttt aaaagggcct aatctgagtc aaaactcttt tttagtctta
    51301 tccatatggt tgcttcattg gtttattatt catacatttt ttctagcagt tgtatatcgt
    51361 agaagagcat ccgatttctt tatggaagtg attcgatttt cttctattaa gctctggatt
    51421 aggtatcaga tttggacctg ttttctttat ggactcattt taatcatggt aaaagttcta
    51481 gtgattcaat ttatgttaca gttaccaaac tgggatatag gagttttgtt tatagttgat
    51541 tctttgaatg cttgtgtgtt agtcttgttt tgctttatgt tatacgcact aggagcgaat
    51601 gtacaaatga actttgcttg cgttagtttc tttttactca tgattgtgtt tggtggtcta
    51661 tttgtaggaa atcgaactaa ttatctattt tatattttga atcgagggaa tggagatata
    51721 ggacgagatt tatttcttca gttgctattt ttagtctttc tttttaaaag cattttctat
    51781 ttcactcgtc aaaagaggag gtttatagaa tgaatgaaat aattacatta aaaaatattc
    51841 agttggaatt aaaaaaaaca tgtgtttttc aaaaccttaa ttttagttgt aaacaggggg
    51901 aaattatagg aattactggt gcgaatggct cagggaaaag tgtattgttt aaattaatag
    51961 ctggtttata tagtccgtct tatggagaag tgttaatcaa tggggaaaat attgttcctg
    52021 agagaaaaat tccagctaat ttgggagctt tgattgaaga acctggtttt ataaattatt
    52081 atagtggctt taagaattta caatatttgg caagcatacg aggagtagtt ggtaatcagg
    52141 aaatcaatga tacactgaaa atagttggtc tatatgagca aaaagaccag aaagttaaaa
    52201 cttattcgct aggtatgagg aaaaagctag ggattgctca agcaattatg gagaatccct
    52261 ctattctttt actagatgaa cctatgaatg ccttggataa atcaagtgta gaaaatatga
    52321 gaacattgtt tagaaagctc tctagtgaaa aagggacaac aattttgatt gctagtcata
    52381 gtgaagagga tattcgtatc ttatgtgata aagtatatgc aatagaagat aaagtatgta
    52441 cactgtgttc agattgaaca tcttagatag actcaggatg ttataggtga aatcttaaaa
    52501 agggattgga tcttaaaatc cagttccttt taagagttaa tgactaatca acttttccac
    52561 cttcaacaac caggtcatct gctttagcag cgtcaccgta ggttgggtga agtgtttttt
    52621 cataggcctt gtggaagaag ttttccttgc ctaatttttc aatatcttta ttgatgaagt
    52681 ctagcaattc ttggttgcct ttttgaactg ctgccgcaat ggtatcggga tcaccgaggg
    52741 aagtaattcc tacttcaaat cctttatttt caagcgccca agctagaact tccgtattgt
    52801 cagttgaaaa ggcatctcca cgtccgtcaa gaagagcttg gtaagagtca ctgtattggt
    52861 cgtatttttg gagtttgatt tctggatgat tcttttcaaa ataagtctca gcagtcgttc
    52921 cttttgtgac aattaaggtt ttaccttcaa gttgtttgac gtctgtaatg agaccagtct
    52981 taggtgatac gacacccaga gaaactttca tatatggaag ggcaaaatca acttgtttct
    53041 tacgttcgtc agttactgta aagttagcaa gagtaatatc taccttgttt gaaatcaagt
    53101 attccgcacg gttggcagca tcgactgaaa tgtatttaac cttgacacca aggtcttgag
    53161 ctagttggtt ccctagttca atatcgtagc cttggtaaga accatcattg tcaacgtagc
    53221 caaacggttt tttatctcca aacacggcga ttcgcagttc accgcttttt ttgttttcat
    53281 cgatagtgcg agctttggca gttgtttttc cagaggatgg accagcgttt ccacctgagc
    53341 tacaagcagt gataaagata agggcaaagg caagtgctaa aacagttaag agtggtttga
    53401 atagtttcat aagaatctcc tttatagata tgagccgaat tggctaaagt caaagacgtt
    53461 taaaaattcc tgggctcgtt tggtttgcgg attggtaaag aaggcttgag ctgttccttc
    53521 ttcagcgatt ttcccttggt cgaggaagat aatccggtca gcaatggctt gggcaaactg
    53581 catttcgtgg gttactaaaa tcatggtacg gccttcttgg accaaatcat tgataagttc
    53641 cagcacctca cgcaccattt ctggatccag cgaagcagtc acctcgtcaa aaaggatgat
    53701 ttctggatgc attaggaggg cacggacaat tgcaacacgt tgcttctgtc caccagataa
    53761 ttgacgggca aagctatgtt gtttatccag caaaccgaca cgctctagta attgcaaagc
    53821 ttcttccgtt acttctttct tgtcccttcc ttgagctttg atagggccta ggatgaggtt
    53881 ttgtaagaca tccagatggg gaaagagttc ataactttga aagaccatgc caatcttttg
    53941 gcgaactagg tgaaaatctt ttttattttc aacgatagac tgaccatcca gaagaatatc
    54001 tccaccttga atactttcta agccgttgag gcaacgaagg agggtacttt tcccacaacc
    54061 agatggccct aggatgacaa caacttccct tttttgattt ctagagaaag tccttggagg
    54121 atgggattgt ctccgaagga tttttttagt tccttgattt ctaagatagt ttcagacatt
    54181 tagtttctcc aatgtttttc taagtgagtg gatagtttgg aaatagggta gcaaactgcg
    54241 aaatataaga ctagaatggt tccataaatc caaaatgaag cagttgggat ggtcaggcga
    54301 ttgctatcga tgatttgttg tccaactttg gtcacttcca caaccccaat caaaacaact
    54361 aatgaagtgg ttttaatcat ccgagtgaca agattgatag cctgcggtag cagtcttctt
    54421 aagacttgtg ggatgatgat gtggtagtaa agttgaacat tagtcaagcc gagtgcctgt
    54481 ccactttcaa actgatgttt agggagagaa gtgatagctc cacgtaccaa gtctcccatt
    54541 tcagctgttc cccagagggt aaaaacgata atagctgaag tctcacctga gatattgata
    54601 ttaaagtttc gagctaagcc aaagtaaacg atgaagagta gcaccagctg gggcatgata
    54661 cgggtaaagt ccagatacaa tcgtgttaaa aatcgtatga ttctagaatg ggaggtcatg
    54721 atgattccca tgactgttcc gaacatcatg gataagagga cagacaggat ggatatctca
    54781 atcgtaacgc ccaatccctg taagattctc aggagattat ttccctgaaa gagtacttgg
    54841 attcccgaat cctgcatggc ggagcctcct ttctatccag ctaaagacca gtgagatggg
    54901 tagcagcatg atcagataag caactaccaa catagctagc gcaatgtctg tctcatagta
    54961 gagaccaatc aaatccttgg cgacatacat gaggtcggcc aaagccactg ctgagaaaac
    55021 agaggtttcc ttgataagga aaatgacatt ggcactaaag gaggggagtg ccaccgctgt
    55081 tgcttgcgga agaaccacat agcgaaagac ctgtagaggt gtcagaccaa tagcgaggcc
    55141 aatctcctgc tgggtttgac tgatggcttc cagcccactt cggaaagatt ctgccatata
    55201 ggatcctcct aaaaagacaa gcccaagcgt tgcacagact tctgaagata ggacaatccc
    55261 gattcgggga agaccgaagt agagaaagaa gagttgaatc aaaaggggcg tattacgtga
    55321 caattcaatg taggctgttg ctacttgcgc caaaacaggg attcgataat gtcggatgat
    55381 actaacgatt aaaccgagca gaaaggatcc caaaattccc caaactgcaa tatacaaagt
    55441 aaacgcttac ttatcaagga gggcatttta tgtcatacaa aacaagcaat gcagaaggtc
    55501 atgtagattt catcaatacc tatgatttgg agccaatggc gcaacaagtt attcctaaag
    55561 cagcatttgg ctatatcgct agtggggcgg aagatacttt cacttctttc cagtgatttt
    55621 agcgtcaggt tctttttagt ttttaaagat tatccgtgaa tttcttgctt atttatgata
    55681 aaatgggggt atcgcaaaaa atgactcatc gtattcaatt ttgagtaaaa ctaggaggat
    55741 cccatgtcta cagaacatat ggaagaacta aatgaccagc agatcgttcg ccgtgaaaaa
    55801 atggctgcgc tccgcgaaca aggaatcgat cctttcggaa aacgttttga acgtactgca
    55861 aattcacaag aattaaaaga taaatatgcc aacctcgata aagaacaatt acacgataaa
    55921 aacgaaacag ctactatcgc aggacgcttg gtaaccaaac gtggtaaagg aaaagttggt
    55981 tttgcccacc ttcaagaccg cgaaggccag attcagatct acgttcgtaa ggatgctgtc
    56041 ggtgaagaaa actacgaaat cttcaaaaaa gcagaccttg gtgacttcct tggtgtcgaa
    56101 ggtgaagtga tgcgtacgga tatgggagaa ctctctatca aggcaaccca catcacacac
    56161 ttgtctaagg ctcttcgtcc tcttcctgag aaattccatg gtttgacaga cgttgaaaca
    56221 atttaccgta aacgttacct tgacttgatt tctaatcgtg aaagctttga acgctttgtc
    56281 actcgttcaa aaatcatctc tgaaatccgt cgttaccttg accaaaaagg attccttgaa
    56341 gtggaaacac ctgttcttca taatgaagcc ggtggtgctg ctgcccgtcc atttatcacc
    56401 caccacaatg cccaaaacat tgacatggtg cttcgtatcg cgactgagct tcacttaaaa
    56461 cgccttatcg tgggtggtat ggaacgtgtc tatgaaattg gccgtatctt ccgtaacgaa
    56521 ggaatggacg ctactcataa ccctgagttc acttctatcg aagtttacca agcttatgca
    56581 gacttccaag acatcatgga cttgactgaa ggcattatcc aacacgctgc taaatcagtc
    56641 aaaggtgatg gcccagtcaa ctaccaaggt actgaaatca agattaacga accatttaag
    56701 cgtgttcata tggtggatgc tatcagagaa attactggtg ttgatttctg gcaagacatg
    56761 actttggaag aagctaaagc tatcgctgct gagaagaaag ttccagttga gaaacactac
    56821 actgaggttg gtcacatcat caatgccttc tttgaagagt ttgttgaaga aactttaatc
    56881 caaccaacct ttgtctatgg acatccagta gctgtatctc cactcgctaa gaaaaatcct
    56941 gaagaccaac gctttactga ccgtttcgag ctctttatca tgactaagga gtacggtaat
    57001 gcctttactg agttgaacga cccaatcgac caacttagcc gttttgaagc ccaagctaaa
    57061 gctaaagaac ttggtgatga tgaagcgaca ggcatcgact atgactacat cgaggctctt
    57121 gaatacggta tgccaccaac aggtggtttg ggaatcggta tcgaccgtct ctgcatgctc
    57181 ctcactgata caacaactat ccgtgatgta ttgctcttcc caacaatgaa ataaattctt
    57241 accctctggc tcttaccagg ggattttttg attcaaaaag agactgaatt taaggagaaa
    57301 atgaagtgta gtatattgaa gttgaaatag tacactttga tttctaagac actgttagaa
    57361 attggtttaa attccctaat caatttgtgc atgttttatt tcattttacg atagtacgct
    57421 gaaacttttc aaaaagtact agaaattgac ttggattccc caattgattt gttcagattc
    57481 actataaata aaaaattaat aagtgggata ggaagttagc gtcaactagg atagtatctt
    57541 gcttaaacag tatatatggg actgatataa gtccataggt cctattagag gatgttctgg
    57601 tgtcttattc acttgttttt tatagtatta gtagatagaa tcagcaaata aaaaagtagt
    57661 tcaaatgcct ggcagcaaaa aatctcaacg attcacttaa tatagactaa ggtttagagt
    57721 ttaaggaaag tagggctagt atattgtatg aaaaaataag atggttttta agcaaaactc
    57781 ttgaaaatga ttgaaaaaag agttattata gttgtatagt aaaaagcaaa cgcttactta
    57841 atcgaggagg acagtttatg tcatacaaaa caagcaatgc agaaggtcat gtagacttca
    57901 tcaataccta tgatttggag ccaatggcgc aacaagttat tcctaaagca gcatttggct
    57961 atatcgctag tggggcggaa gatactttca ctttaagaga gaatatccgc gcctttaacc
    58021 acaaactcat cgttcctcat acactttgcg atgttgaaaa tccaagtaca gagattgaat
    58081 ttgcaggtga aaaactatct tcgccaatca ttatggcacc tgttgcggct cataaattgg
    58141 caaatgaaca gggggaagtg gcgactgcgc gtggtgtgca tgagtttggt tctctttata
    58201 caaccagttc ttactctact gttgaccttc cagagatttc tgaagccctt caagggacac
    58261 ctcattggtt tcaattttac tttagtaagg atgacggtat caaccgccac atcatggacc
    58321 gtgtgaaggc tgaaggttat aaagcgattg tcttgacggc agatgctact gtagggggga
    58381 atcgtgaagt ggataagcgt aatggttttg tcttcccagt tggcatgccg attgttgaag
    58441 aatacctgcc agaaggtgct ggtaaatcaa tggactttgt ttacaaatca gctaaacaac
    58501 gcttgtctcc acgcgatgta gaatttatcg ctgaatactc tggtcttcct gtgtatgtca
    58561 agggaccaca atgccgtgag gacgttgaac gttcgcttgc tgcaggagct tctggtatct
    58621 gggtaaccaa ccacggtggt cgtcaaatcg acggtggacc agctgccttt gactcgcttc
    58681 aagaagtggc agaagcagtt gatagacgtg tgccgattgt ctttgactct ggtgttcgtc
    58741 gtggtcaaca cgtctttaaa gccttggctt caggagcaga cttggtagct attggtcgcc
    58801 cggttattta tggcttggct ctcggtggta gtgtcggtgt gcgtcaagtc tttgaacact
    58861 tgaatgcgga attgaagaca gttatgcaat tgtctggagc tcagaccatt gaagatgtca
    58921 aacactttaa gctccgtcac aatccataca acccaacctt cccagttgac cctcgtgact
    58981 taaaattgta ttgataaaac agattgcctc cactgaatgt ggaggttttt cttcgtgtat
    59041 tggaagaata aatcgctatc gaaaaccata gtaaaatgga ataaaaacag gacaaatcaa
    59101 tcaggatatt taaatcgatt tctaacaatg ttttagaagt agaggggtac tattctagtt
    59161 tcaatctact atatataaag atatttttcg gttaaatatc ttgctaaggc tttcattttt
    59221 tgctatactg gttcagtaat taaataatag agacatttgg ggtgctttaa gctgagatga
    59281 tacccattga acctgataca gttaagactg gcgaagggaa atgtgaaacg ttttttcgta
    59341 tggcgaaatg aacgatctaa tcttgtaagt caactctaat tcttgattgt aattggagtt
    59401 ttttgtttat ataaactgga taggcttgtt tagttagctc tctgcaaatg ctctcgatgt
    59461 gctttaaaag gatcaagtgg aattacgatt ttgcttgata tttcttggtg ttttattaat
    59521 tactaacaag taaagagagg agaagtaaat atggaaacac aagactatgc atttcagcca
    59581 ggtttgactg ttgaagaatt attaaaaagc agtcagaagg attggcaggc tgcaatcaat
    59641 catcgttttg ttaaggaact ttttgcgggg acaattgaga ataaggtctt aaaggactat
    59701 ctgattcaag attatcactt ctttgatgcc ttcttatcca tgctgggtgc ctgcgtagct
    59761 cacgcagata aacttgaatc caagcttcgt tttgccaagc aactaggctt tcttgaagca
    59821 gatgaagatg gttatttcca aaaggctttt aaagagttaa aagtagcaga gaatgactac
    59881 ctagaagtga ctttgcaccc tgtaacaaaa gcctttcagg atctaatgta ttcggcggtg
    59941 gcttcatcag actatgccca tcttttggtt atgctggtca ttgcagaagg tctctattta
    60001 gactggggtt ctaaagattt ggctctacct gaagtctata ttcattcgga atggatcaat
    60061 ctccacagag gtcctttctt tgcagagtgg gttcaatttc tggttgacga actcaatcgt
    60121 gtcggtaaaa atcgagaaga tttgacagaa cttcagcaac gctggaatca agcagtcgct
    60181 ttagaattag ctttttttga tattggttac gatgtctaga gaaagctcat gatatttaca
    60241 aatttgatga ggtaagactc ttcgaaaatc tcttcaaacc acgtcagttc tatccacaac
    60301 ctcaaaacag tgttttgagc aacctgcggt tagcctccta gtttgctctt tgattttcat
    60361 tgagtataaa tcattcaatg taaatgaaaa aaattcttaa cgaaagatag aaataaatcg
    60421 aaatcagtag atcgtataaa gtgaaaagga aggatttcaa tatgacaagt ttaaaattat
    60481 taaaagaaaa agcaccattg gtcatttgta taaccaatga tgtagtaaaa aatttcacag
    60541 caaatggatt agtagcactg agcgtctcac cagccatgag tgagtttcca gcagatttag
    60601 aggatttgtt aaagtatgct ggtggtttat taataaacat aggaacattg acagatgaaa
    60661 attggaaatt ataccaagct gctctgaaaa ttgcggagaa atataatgtc ccagcagttt
    60721 tagatcctgt agcctgtgga gcaggagaat atagaaaaaa agtagcagat gatctaatca
    60781 acaattataa actagcagcg attagaggaa atgctggcga gattgcctct ttagtaggaa
    60841 tagatgtggc atctaaagga gtagatagtg cgggcgtaga taatattgac gaaattgctc
    60901 tagcagcaaa tgagaagttc aatattccaa tagtagtaac aggtgaagtg gatgccattg
    60961 ctgttaatgg agaagtggta acgattcata atggtagtgc tatgatgccg aaagtcattg
    61021 ggacaggatg cttattagga gctgtagtag caagttttat cggactggaa aaaggtcaag
    61081 aattaaaatc attagaaaca gcaatgttag tttacaatat cgctggagaa atggcagaaa
    61141 aacgtccaaa tggacatctt cctgggacat ttaaagttga atttataaat gccttatatg
    61201 aaattacaga tgaagatgtc aagaaattca aaagagtgaa gtaagatgtt tcataaagaa
    61261 ttactaaaac tatattttat ttgtggaacg actacttgcc aaggaaaaaa tctatataca
    61321 gtcgttgagg aagccttaaa aggtggtata accttatttc aatttcgaga aaaaggtgag
    61381 ggtgctttag aaggcttaga aaaacttgag ctagctatcc aaattaaaga actttgtaaa
    61441 aaatacaatg ttccatttat cgttaatgac gatatagatt tagcaatgga aattgacgcc
    61501 gatggcgtac atgtaggtca agatgatatt ggtgttgatg aaattagaaa attgatgcca
    61561 gataaaataa ttggtctttc tataagaaac gaagaagaat ttcaacaatc aaaagttgaa
    61621 tatgtagatt atgtaggtgt tgggcctgta tttgataccc agtcaaaata tgatgctggt
    61681 ggtgctatag gttatgaagg tcttgaattg atgagaaaac tattgccaca aatgccttta
    61741 gttgcaattg gtgggataca gacgaaacat attaaagaca ttataaagac caatatggat
    61801 ggcgtttcaa tcatttcggc aatatcgtat gcaaaaaata tagaaaaaac tgttcgggaa
    61861 atgagtgaac aatagatatc tatccttgga ttctaagtaa gtaggatttt ccttttttaa
    61921 aaagctatgt ccagattcag cttgaatggc taattcatca aacaggtcat ttgttttttg
    61981 caaaagaaag atctgctgtc tttgtgagtg caaggctgta tttccagttc aatttcttga
    62041 actagtataa aatgtgtgtt ataataatca cataattaaa taataaagac atttggggtg
    62101 ctttaggctg agatgatacc cattgaacct gatacagtta agactggcga agggaaatgt
    62161 gaaatgtttt tttcgtatgt cgaaaagaac tgtctaatct tgtaagctga actctaattc
    62221 gtatctgtaa ttagagtttt ttcttttacg aaactggatg aagatgatat agtagattga
    62281 aataagatgc gaacaaatca attagaaaat tcaaattaat ttctagaaat attttagcat
    62341 tcttgtgtac tgttctaaat tcagtctgct atacaaagta tttaggtcgg tcttcttgag
    62401 tgtcccactt ttctttaaaa aaggagtttt ttatgttgaa aaaatggcag ttaaaagatg
    62461 ttatcttgct tgctttcttg tctatctttt ttggtggggt tttcgttggt tcaggatatg
    62521 tgtataatat tttcagtcta ctcttaacac ctcttggttt gcaggccttt gccaatgaaa
    62581 tcctcttcgg tctctggtgt atggctgcgc ccattgctgc catctttgtt ccgagagtcg
    62641 gaagtgcaac gattggagaa gtgctagctg cgcttgctga agtcctttat ggtagccaat
    62701 ttggtctagg agctcttttg tctggcttgg ttcaaggttt gggaagtgaa tttggtttta
    62761 tcgtaactaa gaatcgctat gaaagttggc tctctctaac tgctaatagt attgggatta
    62821 cgcttgttag ctttgtctat gaatacatta agttaggtta ctacgccttt tcccttccgt
    62881 ttgtcctttc cttacttgtg gtacgtttta tttctgttta tttcttctgt accatcttgg
    62941 ttcgtgccat tgtcaaactc tatcatcagt ttgcaactgg aggaaaagca tagatgggtc
    63001 tggaactacg agcgattcag tccccaatct tctctgagcc gtttgatttt acttttcatg
    63061 cgcaagcctt taccttgtta gttgggagca gtggttcagg aaaatccagc ctttttcaag
    63121 tcattgccca agtcagttct cttccctata gtggtcaagt cctgatagat gggagcgagg
    63181 tcagtcagct ttctatcatc gcacgtgtcc agaaggttgg cattctcttt caaaatccca
    63241 atcatcaatt taccatggag aacttgtttg aggagctgat gttcaccttg gagaatatcg
    63301 gctatcacct tcaggaaatt gattctaaaa tagcagaggt tgtccagcaa tgtcgttgcg
    63361 aggcaatctt acaccgtccc attcatcacc tatcaggtgg ggaaaagcaa aaagcagcga
    63421 tggctgtcct ctttgccatg aatcctaggg tatatctctt ggatgagccc ttcgcttcca
    63481 ttgaccgcaa gagcagaatc gagatattgg agattctaaa agagttggct cttgatggga
    63541 agacagttat tttgtgcgac catgatttat ctgactataa agcctatatc gaccatatgg
    63601 ttgaactaag agacggaaaa ctaagggaag tgtttcaaat cccttcctat gagatgacac
    63661 aggttgcttc aaaggaagtt gcttctagcc cggaactatt ccatatggat agaacgactt
    63721 gtgagtttgg aaatcgctcc ctcttttcga ttgctgattt tacattttac caaggaattt
    63781 cctgtatcct gggtgacaat ggtgttggga aatcaaccct ctttcggtct attcttcaat
    63841 ttcaaaagta taaggggcgc attgcatgga aggggacagt cctgaaaaag aaaaagagtt
    63901 tgtaccgtga actgacgagt attgttcagg aagctgagaa gcagtttatc cgagtcagtc
    63961 tgcgagagga gcttcaatta gatggacctg attctgaaag aaatcagcgg atttttcaag
    64021 ctttacgata ttttgatttg gagcaggcag tcgataagag tccctatcaa ttaagtggtg
    64081 gtcagcaaaa aattcttcag ctcttgacca tcttgaccag taaggcttcc gtgatcttgc
    64141 tagatgaacc ttttgcaggt ttggatgata gagcctgcca ttatttttgc aagtggattg
    64201 tggaggagag gaatcaagga agaagttttc tgctcattag tcatcgttta gaccctttga
    64261 tttctgtggt tgattattgg attgagatga ctagtcaggg tcttcggcat gtgaaagaag
    64321 tgaccattac caaaccactt acatctcaga gtagcaatac ccaaggggag gtgagatagt
    64381 atggtcaaag tagcaaccca gacaccgatt atcagtctct tcttgctgat tttatccttg
    64441 gaaacatctt tcattccttc gattgctctg aatctttcgg tagtcgcatt ttgtattctc
    64501 tttatgctct attaccgtcg atttaaaatg ttagcttgga tgatcatact tgccatttta
    64561 ccatcttttg ccaactactg ggcagttcag ttacacggag atgcttcaca ggcagttatg
    64621 cttggaacga gggcctttgt gacagtttgt atcggccttg tctttgtttc ctctatttca
    64681 ctaaaagagc ttctcttgta cttggctcaa aaggggctat cacgctcttg gtcctatgcc
    64741 ttgattgtgg tattcaattc ctttcctctc attcagcaag aaatcaagtc cctcaaagaa
    64801 gcttgcctat tacgtggtca agaactacat ttttggtcgc ccttgattta cagtaaggtt
    64861 ctgatgacag tctttaggtg gcgccatctt tacctgagag ctctatctgc tcacggatat
    64921 gacgaacatg cacagttgaa gaatagctat cggatttttt atattcctaa aaaaacaaaa
    64981 ttaatctacc tgcttttctt tttattgctt caaaccagtc tatttttata aaggagttat
    65041 tatggaattt acagatattg cgatggaatt atccaagaaa gcttggcagg cttcctttca
    65101 tcatcccttt attttacaat tacaagaggg gaatttagaa cctgccattt tccgctatta
    65161 cctgattcag gatgcctact atctgaaggt tttctcagaa atctatcatc ttttggctga
    65221 taagacttca aaccaagaga tgaaaagact cttgaaacaa aatgctcagg gtttagtgga
    65281 gggtgagtta tttattcgcc aacaattttt caaggaattg gaaatcaacg accaggaaat
    65341 ggagcaacat ccaatcgctc caacctgtta tcattacatt tctcatattt ataggcaatt
    65401 tgcagaaccg aacttagcca tagcttttgc aagcttgctt ccttgtcctt ggttatacca
    65461 tgatataggc aaatcactta atcttaaacc atcaccaaat cctctctatc aacaatggat
    65521 tgaaacttat attacggatg agttagagca acagatcaga gaggagggag cattggtcaa
    65581 tcagctctat cgagaaagtg atgagacaga taagcaaaaa atgctagatg ccttccacat
    65641 cagtgttcat atggaagcca agttttggga gatggcttac caacaccaga catggaagag
    65701 cgatttacag tctttagaaa aaggagaaga atagaaacat gagaaagcac caactacaag
    65761 ttcacaaatt gaccatttta tctatgatga ttgcccttga tgtagtcctt acacctatct
    65821 ttcggattga gggaatggca ccgatgtcca gtgtagtcaa tattctagcg ggaatcatga
    65881 tgggacctgt ttatgccttg gctatggcta cagtgacagc ctttatccgt atgacgactc
    65941 aagggattcc gcctttagct ctcacaggag cgacttttgg agcccttcta gcaggtctct
    66001 tttataagta cggtcgaaaa ttttactttt ctgccttggg agaaattgtg ggaacaggta
    66061 ttattggttc tattgtttcc tatcctgtta tggtgctctt tacaggatcg gctgctaagc
    66121 ttagttggtt tatctacact cctcgatttt tcggagcaac cttgattggt acagcgattt
    66181 cctttattgc atttcgattt ttaatcaagc aggaattctt taaaaaagtg caaggatatt
    66241 tctttgctga aaggatagac tgatgcaggc attgacaaat ccttttccta taggctctag
    66301 ttccctcatt cactgtatga ccaatgagat ttcttgtgag atgctggcaa atgggatttt
    66361 ggctctggga tgcaaacctg tcatggcaga tgatccccgt gaagttcttg attttactaa
    66421 gcaaagccag gctctcttca tcaatttggg gcatttgtca gctgagaagg aaaaagcaat
    66481 ccgcatggca gctttgtatg caaaccaatc ttctctcccg atggtagtag atgcggttgg
    66541 cgtaacgact tcatccattc gtaagagctt agttaaagac cttttagact atagacctac
    66601 ggtccttaaa ggaaacatgt cagaaattcg aagtcttgtt ggattaaagc atcacggcgt
    66661 tggggtcgat gcgagtgcta aagatcaaga aacggaggat ttgcttcaag tcttgaaaga
    66721 ctggtgtcag acctatcctg gtatgtcatt cttagtcaca ggtcccaagg accttattgt
    66781 ttcggagaat caagttgctg tacttgagaa tggctgtact gaattagact ggataacagg
    66841 gacaggagac ttggttggag ccttaacagc tgtttttctc agccaaggaa agactggttt
    66901 tgaagcttct tgcttagcag tctcttatct caatatcgct gctgagaaaa tagttgttca
    66961 aggaatggga ttggaagaat ttcgttacca agtactcaat cagctttcgc tcctaagaag
    67021 agatgaaaat tggctagata ccatcaaagg agaggtttat gaatagagaa gcgcttagac
    67081 tatatctggt aaccaatcgc tatcaagatt ccgtggaaag ctttcttgca aaagttgaga
    67141 cggcctgccg ttcaggggtt accatagtcc aattgcgaga aaaaaatctc acaaccaatc
    67201 aatattatca actggcaaaa caagtcaagg aaataacaga tgcctatcag gtccccttga
    67261 taatcgatga tcggttggat gtttgtcttg cggttgatgc tgctggtctg catattggag
    67321 atgacgaact cccagtttcg gttgcccgac aagtcttggg tcctgaaaaa atcctcggtg
    67381 tcaccgctaa aacggttaaa agagctctgg aagcggagaa atcaggtgca gattacttgg
    67441 gtacaggagc cattttccca accaccacca aggaaaatgc gcccattact ctgatatcga
    67501 ccttgaaaac aatttgccaa acggttgcca ttccagtagt tgctattgga ggcttgactt
    67561 cggagaatat tgatcagctt atgggcactg gcatagctgg tgtagctgtt gtacgtgatt
    67621 tgatgcaagc agaagatatt gaggcaaaaa cgcaagcctt tttaaaaaag ttgcataaca
    67681 ttctttccta attaatactc aatgaaaatc aaagagtaaa ctagaaagct agtcgcaggt
    67741 tgctcaaagt actgccttga ggttttagat aagactgacg aagtcagctc aaaacagtgt
    67801 tttgaggctg cagatggaag ctgacgtagt ttgaagagat tttcgaagag tataaaaact
    67861 ctctaattcc cttaaagtgg gaactagaga gttttttttc ttaatcttta aaagttgtat
    67921 ggttgactgg accagaacca tgaccgagtt gaggggcatc ttgaatagct tttgtgataa
    67981 aggccttggc cttatcaact gcctggtaaa gactcttgcc cttggctagt tcagcagtaa
    68041 tcactgcagc aaaggtacat ccagtaccat gggtgtgaca ggtttgaatt cgtgggcttt
    68101 cccagacaaa ttgttcattc ttggtaaaga ggaaatcttt agcaccacct ttgagatgtc
    68161 cgcctttgat aaccacagac tgaggaccaa attcttttaa aatcaggcga ccagcacgct
    68221 gcatgtcttc ggggtcatgg attgaaaaac caacaatctc ttctgcttca ggaagatttg
    68281 gcgtaataat agttgctaga ggtagtaagt ttgttttgag atagtctcta gcacttgagt
    68341 caatcaaggc atctccactt gtagcaacca taacaggatc aaggacatag ggacaatcca
    68401 gttttttaag atagggttgg atgatttcca tgatttcagt agtagccaac attccagttt
    68461 ttacagcctg aggtggaata tcagaaaaga tactctccaa ttgggctttc aacatttgag
    68521 gagaaacgtg ctcgattagc tgaacacctc tggtattttg agcgacaaga ctggttacaa
    68581 cagccattcc atagacatct ctcacttgga atgactttaa atctgccata atgccagcac
    68641 caccactagg gtcagtccct gcaatggtca aagcaacggg taaataagtc atctaaaacc
    68701 tcccaaccaa ctgaagtttg tattcttaag aacaagctag tctgttttag aaaggcaata
    68761 gaagactgct agtccccatt atcatttcca cttccatcag ctagcattac ctagattgag
    68821 tcagagggat ctaacagtcg ttaatctcag ctttacgcac ccctagtggt tgttttcttt
    68881 tttctttagt atagcatatt tttattgttt atatagtaat atttgactgc aaatcgtttt
    68941 tcaaatgatt aaaattctat ttttcgaaga aaatattatt tctataattg acaaatgtag
    69001 attttaagag tatactgatg agtgtaattg acaaatgtag attttggagg tatggtaatg
    69061 cagatttcag atgcagaatg gcaggtcatg aagattattt ggatgcaggg ggagcagacc
    69121 agtacagatt tgattagggt tttggcagag cggttcgact ggtccaagtc aacggttcag
    69181 acacttttgt ctcggctggt tgagaaagag tgtttgactc ggaaaaaaga aggcaagtcc
    69241 tttgtttatt cagccctttt aactctagac caaagtcggg atttacttgt ccaagatatc
    69301 aaagacaagg tttgttcccg taggattagg aatttgttgg ctgacttgat tgtagaatgt
    69361 gaatttactc agactgactt ggaagacttg gaagctgtga tttcagagaa gaaatcaagt
    69421 gctgtaacag aagtaagatg taattgtatg taaaggagac gtcatgttaa atagtattgt
    69481 aaccattatt tgtattgccc ttatcgcgtt tatcttgttt tggtttttca aaaagcctga
    69541 aaaatctgga caaaaagccc agcaaaaaaa tggataccaa gagattcgag tggaagtcat
    69601 gggaggctat actcctgagt tgattgtcct caagaaatca gtgccagcac gcattgtatt
    69661 tgaccgcaag gatccttcac catgtctgga tcaaattgtt tttccagatt ttggtgtaca
    69721 tacgaacctg ccaatggggg aagagtatgt agtggaaatc acgcctgagc aggctggaga
    69781 gtttggcttt gcttgtggta tgaacatgat gcacggcaag atgattgtag agtaggtgga
    69841 gactatgaca gaaattgtga aagcaagctt agaaaatggc attcaaaaaa tccgtatccg
    69901 agctgaaaaa ggctatcatc cagcccatat ccagcttcaa aagggaattc cagctgagat
    69961 tacctttcat cgtgctactc cttcaaactg ttataaggaa attctgtttg aagaagaagg
    70021 tatcttggaa ccaatcggcg tagatgagga gaaagtcatt cgttttacac ctcaagaatt
    70081 agggcgacat gaattttctt gtggcatgaa gatgcaaaag ggaagctata cagtcgttga
    70141 gaagactcga aaatctctat ctctcctgca acgtttttgg attactagta tctttactgt
    70201 gcctcttgtg attctcatga ttgggatgtt gacaggtagc attagtcatc aagtcatgca
    70261 ttggggaact tttctagcaa caacgactat tatgttagtt gcaggtaagc catatatcca
    70321 gagtgcttgg gccagtttta aaaagcacaa tgccaacatg gataccttgg ttgcgctggg
    70381 aactctagtg gcttatttct atagcctagt tgctctcttt gctggtctcc ctgtttactt
    70441 cgaaagtgct ggatttatcc tctttttcgt tcttttggga gcagtttttg aggaaaaaat
    70501 gagaaaaaat acgtcccaag ctgtggagaa attactggac ttgcaagcta aaaccgcaga
    70561 agtcttgagt gatgatagtt atgtccaagt tcctttggaa caagtcaagg taggcgacct
    70621 gattcgagtg cgtcccggtg aaaagattgc tgttgatggt gtcgtagtag aaggtgtctc
    70681 tagtattgac gaatccatgg tgacaggtga gagtctgcct gtggacaaga cagttggaga
    70741 tactgtcatt ggctcaacca tcaatcatag tggaacgctt gtctttagag cagaaaaagt
    70801 tggctcagag actgttttgg ctcagattgt ggattttgtg aagaaagctc agacaagtcg
    70861 tgcgccgatt caagacttga cggataagat ttcagggatt tttgtcccag tagttgtcat
    70921 tttaggaatc atgacctttt gggtttggtt cgtcttgctc agggatagtg tggttgtgct
    70981 tggagctagc tttgtgtcct ctcttctcta cggagtggcg gttttgatta tcgcctgtcc
    71041 ttgtgccttg ggacttgcaa caccgacagc ccttatggtg gggacaggac gtagtgccaa
    71101 gatgggggtt ctcctcaaaa atggaactgt cttacaggaa atccagaaag ttcaaaccct
    71161 tgtctttgat aagaccggga ctttgacgga agggaaacct gtggtaacag atgtcatcgg
    71221 cgacgaagta gaagtgttta ggttggcagc ctccttggaa gatgcttctc aacacccact
    71281 ggctgaggct atcgttaagc gagcgagtga agctggactt gagtttcaaa ctgttgaaaa
    71341 ttttcagacc ttgcacggga aaggtgtttc agggcgaatc aatggaaaac aagttttact
    71401 tggaaatgct aaaatgctgg atggcatgga tatttctaat acttatcaag ataaactaga
    71461 agaactagaa aaagaagcta agacagttgt gttcttagct gttgacaatg aaatcaaagg
    71521 cttgcttgct ttgcaagata ttcctaagga aaatgctaag ctagccatca gtcagctaaa
    71581 aaaacgtggt ctccgaacag tcatgctgac aggagacaat gctggtgtgg cgcgtgctat
    71641 tgcagatcaa atcggaattg aagaggtcat tgcaggcgtc ttgccagaag aaaaagccca
    71701 tgaaatccat aaactgcaac agtcagggaa agtagccttt gttggggacg gtatcaatga
    71761 cgctcctgcc cttagtgtag cagatgtggg gattgctatg ggagctggaa cagatatcgc
    71821 catcgagtca gcagatttgg tgttgacaac caataatctt ttaggagtgg ttcgtgcctt
    71881 tgatatgagt aagaaaacct ttcatcgaat tctactcaat cttttctggg cttttatcta
    71941 caatgttgtc ggaattccga ttgcagcagg agtcttttca ggtgttggac tggctctcaa
    72001 cccagaattg gcaggtctag ccatggcctt tagttctgta tctgttctga ccagttccct
    72061 tctgttaaat tttagtaaaa tagactaaaa gtaatgacta gatttctttg ttataaaaca
    72121 gaaatgacag aactttcaaa actgtattag aatagagaat agagagtttt gagcagattt
    72181 ttagaaaagt cagcataaat atgatacagt ggaatagtaa aaatttggag aacgtttcca
    72241 attctatgta atcgtattct ccaaatctaa aaaaattgaa ggagagttat cattatgact
    72301 caagggaaaa ttactgcatc tgcagcaatg cttaacgtat tgaaaacatg gggcgtagat
    72361 acaatctacg gtatcccatc aggaacactc agctcattga tggacgcttt ggctgaagac
    72421 aaagatatcc gcttcttaca agttcgccac gaagagacag gtgctcttgc agcggttatg
    72481 caagctaaat tcggcggctc aatcggggtt gcagttggtt caggtggtcc aggtgcgact
    72541 cacttgatta acggtgttta cgatgcagct atggataaca ctccattcct agcgatcctt
    72601 ggatcacgtc cagttaacga attgaacatg gatgctttcc aagagcttaa ccaaaaccca
    72661 atgtacaacg gtatcgctgt ttacaacaaa cgtgtagctt acgctgagca attgccaaaa
    72721 gtaattgacg aagcctgccg tgctgcaatt tctaaaaaag gtccagctgt tgttgaaatt
    72781 ccagtaaact tcggtttcca agaaatcgac gaaaactcat actacggttc aggttcatac
    72841 gaacgctcat tcatcgctcc tgctttgaac gaagttgaaa tcgacaaagc tgttgaaatc
    72901 ttgaacaatg ctgaacgccc agttatctat gctggatttg gtggtgttaa agctggtgaa
    72961 gtgattactg aattgtcacg taaaatcaaa gcaccaatca tcacaactgg taaaaacttt
    73021 gaagctttcg aatggaacta tgaaggtttg acaggttctg cttatcgtgt tggttggaaa
    73081 ccagccaacg aagtggtctt tgaagcagac acagttcttt tccttggttc aaacttccca
    73141 tttgctgaag tttacgaagc attcaagaac actgaaaaat tcatccaagt tgatatcgac
    73201 ccttacaaac ttggtaaacg tcatgccctt gacgcttcaa tccttggtga tgctggtcaa
    73261 gcagctaaag ctatccttga caaagtaaac ccagttgaat caactccatg gtggcgtgca
    73321 aacgttaaga acaaccaaaa ctggcgtgat tacatgaaca aactcgaagg taaaactgag
    73381 ggtgaattgc aattgtatca agtttacaat gcaatcaaca aacatgctga tcaagacgct
    73441 atctactcaa tcgacgtagg taacactact caaacatcta ctcgtcatct tcacatgaca
    73501 cctaagaaca tgtggcgtac atctccactc tttgcgacaa tgggtattgc ccttcctggt
    73561 ggtatcgctg ctaagaaaga caatccagat cgccaagtat ggaacatcat gggtgacgga
    73621 gcattcaaca tgtgctaccc agacgttatc acaaacgttc aatacgacct tccagttatc
    73681 aaccttgtct tctcaaatgc tgagtacggc ttcatcaaga acaaatacga agatacaaac
    73741 aaacacttgt ttggtgtaga cttcacaaac gctgactacg ctaaaattgc ggaagctcaa
    73801 ggagctgttg gattcacagt tgaccgtatc gaagacatcg atgcagttgt tgcagaagct
    73861 gttaaattga acaaagaagg taaaactgtt gtcatcgatg ctcgcatcac tcaacaccgt
    73921 ccacttccag tagaagtact tgaattggat ccaaaacttc actcagaaga agctatcaaa
    73981 gccttcaagg aaaaatacga agcagaagaa ctcgtaccat tccgtctctt cttggaagaa
    74041 gaaggattgc aatcacgcgc aattaaataa ttcctctcgc cgaaaatcaa atatgaaact
    74101 tgtagacttt attctttgat tttcatagag cagaacttta aaagatcgga gcaatccggt
    74161 ctttttatgg aggagagtaa atgaatttaa atcaattaga tattatcgtt tcaaatgttc
    74221 cccaagtctg tgctgacttg gagcatatct tggataaaaa ggcagattat gctgatgatg
    74281 gttttgctca gtttacgatt ggtagtcact gccttatgtt gtcacaaaat catttggttc
    74341 ctttggaaaa ctttcagtca ggaatcatta ttcatatcga ggttgaggat gtaggccaga
    74401 actacaaacg gttgaacgag cttggtataa aggttttaca cggaccaact gtaaccgatt
    74461 ggggaacaga gtccttatta gttcaaggac ctgctggtct agtgcttgat ttttatcgta
    74521 tgaaataggt cgcgtagtca tttgacgtta accttagcta tttttaaaat agaaattgag
    74581 aatatgaact attgaaagat cggagcaatc cggtcttttt atataaagaa attgcatata
    74641 atactcatct gctttcaaaa agcattctag tccatctccg attaacgatg gactttatca
    74701 cctccttctc cagtccttgt atgacatctt gaagttgatt catgacatct tccaaagttc
    74761 gaaaggcttt attcttaaat ccacgtttac gaa
//
LOCUS       DBERMR010000003        71814 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.003, whole genome shotgun sequence.
ACCESSION   DBERMR010000003 DBERMR010000000
VERSION     DBERMR010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 71814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71814
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.003"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(166..324)
                     /locus_tag="ACL8A1_000221"
                     /pseudo
     CDS             complement(166..324)
                     /locus_tag="ACL8A1_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(702..1391)
                     /gene="rplA"
                     /locus_tag="ACL8A1_000222"
     CDS             complement(702..1391)
                     /gene="rplA"
                     /locus_tag="ACL8A1_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HGS1201076.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(1599..2024)
                     /gene="rplK"
                     /locus_tag="ACL8A1_000223"
     CDS             complement(1599..2024)
                     /gene="rplK"
                     /locus_tag="ACL8A1_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HGS1201077.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            2156..2293
                     /locus_tag="ACL8A1_000224"
     CDS             2156..2293
                     /locus_tag="ACL8A1_000224"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201078.1"
                     /translation="MKIKEQTRKLAAGCSKYSFEVADKTDEVSSKHCFEVVNRTDAVS
                     R"
     gene            complement(2333..3049)
                     /gene="ldcB"
                     /locus_tag="ACL8A1_000225"
     CDS             complement(2333..3049)
                     /gene="ldcB"
                     /locus_tag="ACL8A1_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase LdcB/DacB"
                     /protein_id="HGS1201079.1"
                     /translation="MKKRYLVLTALLALSLAACSQEKAKNEDGTAKTEQTAKADGTVG
                     SKSQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKAAADRYSARPGYSEHQTGLAFD
                     VIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            complement(3052..3522)
                     /locus_tag="ACL8A1_000226"
     CDS             complement(3052..3522)
                     /locus_tag="ACL8A1_000226"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HGS1201080.1"
                     /translation="MCLICQRIDLIKKEENPYFVKELETGYLVIGDHQYFAGYSLFLA
                     KEHVTELHHLKKETRLRFLEEMSLVQEAVAKAFAAEKMNIELLGNGDAHLHWHLFPRR
                     TGDMNGHGLKGRGPVWWVPFEEMTAETCQVKPEEIKRLVECLSSEVDKLLEIKE"
     gene            complement(3524..4561)
                     /locus_tag="ACL8A1_000227"
     CDS             complement(3524..4561)
                     /locus_tag="ACL8A1_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HGS1201081.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGEKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDPRTVVTDTGFIKSRHLDDKVSAAILLNLLRIYMEEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVTLA
                     KNQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYLKSGLVD"
     gene            complement(4659..5984)
                     /gene="brnQ"
                     /locus_tag="ACL8A1_000228"
     CDS             complement(4659..5984)
                     /gene="brnQ"
                     /locus_tag="ACL8A1_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HGS1201082.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKVSLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(6155..6442)
                     /locus_tag="ACL8A1_000229"
                     /pseudo
     CDS             complement(6155..6442)
                     /locus_tag="ACL8A1_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            complement(6602..7180)
                     /locus_tag="ACL8A1_000230"
     CDS             complement(6602..7180)
                     /locus_tag="ACL8A1_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HGS1201083.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            complement(7234..8634)
                     /gene="pepV"
                     /locus_tag="ACL8A1_000231"
     CDS             complement(7234..8634)
                     /gene="pepV"
                     /locus_tag="ACL8A1_000231"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HGS1201084.1"
                     /translation="MTAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVVSVSLSEHGHTPHYVPMEDPLV
                     QTLLNIYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(8647..9252)
                     /locus_tag="ACL8A1_000232"
     CDS             complement(8647..9252)
                     /locus_tag="ACL8A1_000232"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HGS1201085.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDDKLEPSYRLPVDEIIEKR"
     gene            9423..10223
                     /locus_tag="ACL8A1_000233"
     CDS             9423..10223
                     /locus_tag="ACL8A1_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS1201086.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKESSSQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTYLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATIDFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSTDSPSN
                     YIVFSSDQKEFKEKFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            complement(10405..11238)
                     /locus_tag="ACL8A1_000234"
     CDS             complement(10405..11238)
                     /locus_tag="ACL8A1_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HGS1201087.1"
                     /translation="MNHKIAILSDVHGNATALEAVIADAKNQGASEYWLLGDIFLPGP
                     GANDLVALLKDLPITASVRGNWDDRVLEALDGQYGLEDPQEVQLLRMTQYLMERMDPA
                     TIVWLRSLPLLEKKEIDGLRFSISHNLPDKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSQGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELLNIQFRKV
                     AYDYEAELELAKSKGLPFIEMYEELRRDDNYQGHNLELLASLIEKHGYVEDVKNFFDF
                     L"
     gene            complement(11228..13072)
                     /gene="uvrC"
                     /locus_tag="ACL8A1_000235"
     CDS             complement(11228..13072)
                     /gene="uvrC"
                     /locus_tag="ACL8A1_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HGS1201088.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEAYFKSMAQEVSDFLKGQDDKIIDDLKSKMAVAAQSMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDSKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     SVDEIVEVGVPRVVAEAVQRKLNPQGEALPQVAEERVDYQTEGNHNEP"
     gene            complement(13112..13813)
                     /locus_tag="ACL8A1_000236"
     CDS             complement(13112..13813)
                     /locus_tag="ACL8A1_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M57 family metalloprotease"
                     /protein_id="HGS1201089.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            complement(13892..15124)
                     /gene="murN"
                     /locus_tag="ACL8A1_000237"
     CDS             complement(13892..15124)
                     /gene="murN"
                     /locus_tag="ACL8A1_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HGS1201090.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNSIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSDYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(15129..16349)
                     /gene="murM"
                     /locus_tag="ACL8A1_000238"
     CDS             complement(15129..16349)
                     /gene="murM"
                     /locus_tag="ACL8A1_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HGS1201091.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFVIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKELEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRISKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(16461..17240)
                     /locus_tag="ACL8A1_000239"
     CDS             complement(16461..17240)
                     /locus_tag="ACL8A1_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGS1201092.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALATNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIKDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(17309..18970)
                     /locus_tag="ACL8A1_000240"
     CDS             complement(17309..18970)
                     /locus_tag="ACL8A1_000240"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HGS1201093.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPEHIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(19145..21367)
                     /gene="recJ"
                     /locus_tag="ACL8A1_000241"
     CDS             complement(19145..21367)
                     /gene="recJ"
                     /locus_tag="ACL8A1_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HGS1201094.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEERENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GGASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAIVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            21644..22402
                     /locus_tag="ACL8A1_000242"
     CDS             21644..22402
                     /locus_tag="ACL8A1_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGS1201095.1"
                     /translation="MALVEFKNVEKYYGDYHALRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLEAVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKTVLENVT
                     LAPIKVLGIDKKEAEKTAKKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLARDGMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDTTDVDNFFDNPSEPRAQQFLSKIINHESDKVK"
     gene            22413..23207
                     /locus_tag="ACL8A1_000243"
     CDS             22413..23207
                     /locus_tag="ACL8A1_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HGS1201096.1"
                     /translation="MKKKFFLSALLISLFGLAAAKPVQADTSIADIQKRGELVVGVKQ
                     DVPNFGYKDPKTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQGDMDIA
                     TFTITDERKKLYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITE
                     LGKKKGLKFKFVELGSYPELITSLHAHRIDAFSVDRSILSGYTSKRTALLDDSFKPSD
                     YGIVTKKSNTEFNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            23220..23897
                     /locus_tag="ACL8A1_000244"
     CDS             23220..23897
                     /locus_tag="ACL8A1_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGS1201097.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHTIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQTFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            23907..24566
                     /locus_tag="ACL8A1_000245"
     CDS             23907..24566
                     /locus_tag="ACL8A1_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGS1201098.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNSVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            24862..26052
                     /locus_tag="ACL8A1_000246"
     CDS             24862..26052
                     /locus_tag="ACL8A1_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HGS1201099.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTNNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(26373..27254)
                     /locus_tag="ACL8A1_000247"
     CDS             complement(26373..27254)
                     /locus_tag="ACL8A1_000247"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HGS1201100.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(27395..28723)
                     /gene="vncS"
                     /locus_tag="ACL8A1_000248"
     CDS             complement(27395..28723)
                     /gene="vncS"
                     /locus_tag="ACL8A1_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HGS1201101.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIDFPKVVQD"
     gene            complement(28720..29376)
                     /gene="vncR"
                     /locus_tag="ACL8A1_000249"
     CDS             complement(28720..29376)
                     /gene="vncR"
                     /locus_tag="ACL8A1_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HGS1201102.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASIAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(29425..29508)
                     /gene="pep27"
                     /locus_tag="ACL8A1_000250"
     CDS             complement(29425..29508)
                     /gene="pep27"
                     /locus_tag="ACL8A1_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HGS1201103.1"
                     /translation="MRKEFHNVLSSGQLLTDKRPARDYNRK"
     gene            complement(29688..31067)
                     /gene="vex3"
                     /locus_tag="ACL8A1_000251"
     CDS             complement(29688..31067)
                     /gene="vex3"
                     /locus_tag="ACL8A1_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HGS1201104.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(31119..31766)
                     /gene="vex2"
                     /locus_tag="ACL8A1_000252"
     CDS             complement(31119..31766)
                     /gene="vex2"
                     /locus_tag="ACL8A1_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HGS1201105.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQINYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(31779..33056)
                     /locus_tag="ACL8A1_000253"
     CDS             complement(31779..33056)
                     /locus_tag="ACL8A1_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1201106.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGNSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
     gene            complement(33193..33477)
                     /locus_tag="ACL8A1_000254"
                     /pseudo
     CDS             complement(33193..33477)
                     /locus_tag="ACL8A1_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(33620..33985)
                     /locus_tag="ACL8A1_000255"
     CDS             complement(33620..33985)
                     /locus_tag="ACL8A1_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201107.1"
                     /translation="MNNVDNQPATEVPSSGITVKLNTKDNAGNWTSASNKKEITVKIV
                     SAKPTYPDKILVKNPDNIKDTEKNAIIEKLKEANKNHPAGAPTFAKGEGEHANDIVAT
                     YSDGTTYYVPLNDVTKYAR"
     gene            complement(34461..35345)
                     /locus_tag="ACL8A1_000256"
     CDS             complement(34461..35345)
                     /locus_tag="ACL8A1_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS1201108.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVVLLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDMIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            complement(35349..35735)
                     /locus_tag="ACL8A1_000257"
     CDS             complement(35349..35735)
                     /locus_tag="ACL8A1_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HGS1201109.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            complement(35728..37071)
                     /gene="cysS"
                     /locus_tag="ACL8A1_000258"
     CDS             complement(35728..37071)
                     /gene="cysS"
                     /locus_tag="ACL8A1_000258"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HGS1201110.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLDLGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            complement(37153..38037)
                     /locus_tag="ACL8A1_000259"
     CDS             complement(37153..38037)
                     /locus_tag="ACL8A1_000259"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1201111.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSTNT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            complement(38049..38666)
                     /gene="cysE"
                     /locus_tag="ACL8A1_000260"
     CDS             complement(38049..38666)
                     /gene="cysE"
                     /locus_tag="ACL8A1_000260"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HGS1201112.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KHGFKLLARMYSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            complement(38682..40895)
                     /gene="pnp"
                     /locus_tag="ACL8A1_000261"
     CDS             complement(38682..40895)
                     /gene="pnp"
                     /locus_tag="ACL8A1_000261"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HGS1201113.1"
                     /translation="MAKQVFQTTVAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQVVKDQVT
                     AVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDLEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHQKDHKPKKEFTETPKDSE"
     gene            complement(41582..42448)
                     /gene="metF"
                     /locus_tag="ACL8A1_000262"
     CDS             complement(41582..42448)
                     /gene="metF"
                     /locus_tag="ACL8A1_000262"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HGS1201114.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNDNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQTPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            complement(42512..44761)
                     /gene="metE"
                     /locus_tag="ACL8A1_000263"
     CDS             complement(42512..44761)
                     /gene="metE"
                     /locus_tag="ACL8A1_000263"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HGS1201115.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVAAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFCLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            complement(45022..45288)
                     /locus_tag="ACL8A1_000264"
     CDS             complement(45022..45288)
                     /locus_tag="ACL8A1_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS1201116.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGKTSPQLDTVLKVLASLGKTLAIVPLEQGKS"
     gene            complement(45414..45817)
                     /locus_tag="ACL8A1_000265"
                     /pseudo
     CDS             complement(45414..45817)
                     /locus_tag="ACL8A1_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            45973..46974
                     /locus_tag="ACL8A1_000266"
     CDS             45973..46974
                     /locus_tag="ACL8A1_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGS1201117.1"
                     /translation="MIKTFLSALSVILFSIPIITYSFFPSSNLNIWLSTQPILAQIYA
                     FPLATATMAAILSFLFFFLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NALPPFERGTWNSQSPKLFNATIDHILLPKNHYYVRDLDIVSFQNSDHRCIFTEITF"
     gene            complement(47042..49447)
                     /gene="pheT"
                     /locus_tag="ACL8A1_000267"
     CDS             complement(47042..49447)
                     /gene="pheT"
                     /locus_tag="ACL8A1_000267"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HGS1201118.1"
                     /translation="MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEEERQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPENAVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVSIGTDK
                     APYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDT
                     FEGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKS
                     SRVVLEAAVFNGKSIRKTSGHLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VSVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTVTQKLRRQVRTIAE
                     GAGLTEIITYALTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFTRLGLQVTYTATSEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSAIEGALQPAVPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNA
                     EVR"
     gene            complement(49524..50033)
                     /locus_tag="ACL8A1_000268"
     CDS             complement(49524..50033)
                     /locus_tag="ACL8A1_000268"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1201119.1"
                     /translation="MIRKVEMADVEVLAKIAKQTFRETFAYDNTEEQLQEYFEEAYSL
                     KTVSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            complement(50033..51079)
                     /gene="pheS"
                     /locus_tag="ACL8A1_000269"
     CDS             complement(50033..51079)
                     /gene="pheS"
                     /locus_tag="ACL8A1_000269"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HGS1201120.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFNQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            complement(51671..53085)
                     /locus_tag="ACL8A1_000270"
                     /pseudo
     CDS             complement(51671..53085)
                     /locus_tag="ACL8A1_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            complement(53098..54936)
                     /locus_tag="ACL8A1_000271"
     CDS             complement(53098..54936)
                     /locus_tag="ACL8A1_000271"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HGS1201121.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGAPVTPASYGSSVVPILIMV
                     WLMKYIEKMIAKLTPAVTKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLILPNNQTIEHSKELLVIL"
     gene            complement(54954..55793)
                     /gene="licT"
                     /locus_tag="ACL8A1_000272"
     CDS             complement(54954..55793)
                     /gene="licT"
                     /locus_tag="ACL8A1_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HGS1201122.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKIGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGVIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTK
                     NLD"
     gene            complement(56094..58669)
                     /locus_tag="ACL8A1_000273"
                     /pseudo
     CDS             complement(56094..58669)
                     /locus_tag="ACL8A1_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442363.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            complement(59166..59969)
                     /locus_tag="ACL8A1_000274"
     CDS             complement(59166..59969)
                     /locus_tag="ACL8A1_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HGS1201123.1"
                     /translation="MQPTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATVYHHTVDASTEEADLVSLRIVELLSRRGFRFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNIPFQQHSKY
                     FRDALVLDNAKILQRRPEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
     gene            complement(60187..60328)
                     /locus_tag="ACL8A1_000275"
                     /pseudo
     CDS             complement(60187..60328)
                     /locus_tag="ACL8A1_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155197.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(60349..61953)
                     /locus_tag="ACL8A1_000276"
     CDS             complement(60349..61953)
                     /locus_tag="ACL8A1_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGS1201124.1"
                     /translation="MSEYWFSTNVDQIDEVDGKQCLIYSYYNIKASRNVEVLKGRSGT
                     KKGLDYWEPYAPQKQYEMERLPKNKYIGSSSTDRWDGIEKNVVFCDCKEYVSAFDLFF
                     YHYNFKKISTQRSKQDFIRLRSKPVADILKNNTSSYTRYKKEMVIDNVKVDDKVCEII
                     SEIMDESYTDIQILTHKLYSKGDDIKASKTIWMKKSGKEYSEAFAGTGEARIILLVND
                     IVNAQSNSLILIDEPEISLHPSAIYKFKEFLLQECLNKKHQIIITTHSTQLIKDFPRE
                     AVKLLVKNGEKVDVIENIDYQDAFFELGDVYHSRKMIYVEDRLAKYILEFVITHSGSE
                     NLKQNLVVRYIPGGANQIICNNILNSSYLDSDNHYFWLDGDQNTNVSESNNLMNYLEN
                     GVVISDKIPESDNKNLDDIIKLITGCPIKFNVSGNKGQKNNIELIAKQRSFIDYWAKY
                     VSYLPFPTPEFFLANLCNSVDREGYDFSKDGNGKEYFRKKTQVALGIENITSEDIFQE
                     QRRAVSKIQPESSMFQCIKEKLEALF"
     gene            complement(62162..63253)
                     /locus_tag="ACL8A1_000277"
     CDS             complement(62162..63253)
                     /locus_tag="ACL8A1_000277"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HGS1201125.1"
                     /translation="MGKIVAIDLFSGAGGTTSGLKKSGIDVQVAVEIDSVAVKTYKLN
                     NPEVSVIDKDINVVSGDEVKEYLKIDTDDKLMLVACPPCQGFSTIGTNNENDERNQLV
                     FQFLRLINELNPDFLLMENVVGMTRTRNEKIFKKFLTSIDNNYSVIYDILNTADYGVP
                     QSRKRLVLHGIRKEILDNTNLTLNLPSKTHSNIPELDLLSWINADIIMDLPPIEGGEE
                     YIGEGIYNHVANKLSNLNIERITHIRNNGGSRTSLPKSLELNCHKNRLGHSDVYGILD
                     INKPSITITGGCMSYSKGRFGHPTQNRALSAREAARLQSFDDDYIFVGNRGQLAKQIG
                     NAVPVKLAQASGNYFMSVYREIANGQSDN"
     gene            complement(63334..65985)
                     /locus_tag="ACL8A1_000278"
     CDS             complement(63334..65985)
                     /locus_tag="ACL8A1_000278"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HGS1201126.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ITRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAMANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNEAGEIYVGEEAPEGDDWTQDSDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLPLEQATANVEKVATGEAGNVTDRWILHNLNETIGKA
                     TANFDKFEFGVAGHILYNFIWDEFADWYVELTKEVLYSDNEEEKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAAYPTVNLAFEDLAAHTGVESLKNLIRAVRNA
                     RAEVNVAQSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIYLPLADLLNVEEELARLEKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     ERDKQADYQAKYDATVARIDEMKKLVK"
     gene            complement(65978..66556)
                     /locus_tag="ACL8A1_000279"
     CDS             complement(65978..66556)
                     /locus_tag="ACL8A1_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGS1201127.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLMDSTLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYHLIYFKQIQSLLDDYHQEI
                     LFVELETSLEECIRRNRTENRLKYKPLKRHIEVSEREIIETAETLQLNSQHQPNELNH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            complement(66556..67116)
                     /locus_tag="ACL8A1_000280"
     CDS             complement(66556..67116)
                     /locus_tag="ACL8A1_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HGS1201128.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHILPERNQKENLPAGYGIVVKVTDKIVGSVDFNHRHEDDVLEIGY
                     TLHPDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSKRVAEKLGFTLEARI
                     RDRKDAQGNRCDDLRYALLESEWEVI"
     gene            complement(67103..67552)
                     /locus_tag="ACL8A1_000281"
     CDS             complement(67103..67552)
                     /locus_tag="ACL8A1_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HGS1201129.1"
                     /translation="MIGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEK
                     LERLGVHYEISERTQIPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPELLDE
                     KGHFKNQLFAPTYFMGDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            complement(67965..68360)
                     /locus_tag="ACL8A1_000282"
     CDS             complement(67965..68360)
                     /locus_tag="ACL8A1_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HGS1201130.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLDLKGIDPATIKKLKENGIKFK"
     gene            complement(68456..68710)
                     /locus_tag="ACL8A1_000283"
     CDS             complement(68456..68710)
                     /locus_tag="ACL8A1_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HGS1201131.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKVGKGFHDLPEGLFGERNY"
     gene            complement(68720..70054)
                     /locus_tag="ACL8A1_000284"
     CDS             complement(68720..70054)
                     /locus_tag="ACL8A1_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HGS1201132.1"
                     /translation="MTDERIHILRDILLELHNGASPESVQDRFDATFTGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYESMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQIFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKAIMKGLREGTKDKY
                     EMWFKSESRGKFVHITYAAVHDENGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            complement(70054..70287)
                     /locus_tag="ACL8A1_000285"
     CDS             complement(70054..70287)
                     /locus_tag="ACL8A1_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HGS1201133.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            70318..70470
                     /locus_tag="ACL8A1_000286"
     CDS             70318..70470
                     /locus_tag="ACL8A1_000286"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201134.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEVVDRTDEVSSKHRFEVVDRTDEVS
                     NIYTAR"
     gene            complement(70601..70753)
                     /locus_tag="ACL8A1_000287"
     CDS             complement(70601..70753)
                     /locus_tag="ACL8A1_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201135.1"
                     /translation="MKFFWGLLAILFIKPIIGIVKFFWMIISFAVQLLFYKIVFKILD
                     WLFKLI"
     gene            complement(70750..70932)
                     /locus_tag="ACL8A1_000288"
     CDS             complement(70750..70932)
                     /locus_tag="ACL8A1_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201136.1"
                     /translation="MGLIKTLAKIYGNYFLTVQGVKVMKTIKKDDNAVVGLGKLFIAD
                     KLMDTARWLIKPEERE"
     gene            complement(70934..71713)
                     /locus_tag="ACL8A1_000289"
     CDS             complement(70934..71713)
                     /locus_tag="ACL8A1_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201137.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELGPQDIPTL
                     LEQGPTSLLSQEILDRVANACIRDNVLLASGTSVLAGLPGGLAMAITIPADVAQFYGF
                     SLKLAQELGYIYGYEDLWASRDELSEDAQNTLLLYLGVMLGVNGTAALLRAGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLRIFGVNLTKGGLAKGMGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIEGE"
BASE COUNT        20822 a        16055 c        12978 g        21959 t
ORIGIN      
        1 atacgcacgg caaggcgacg ttgacgtggt ttgaagagat tttcgaagag tattagaaag
       61 ctcgatggac tattccagat tcattccact agatttcgaa atcgagtagg ggataatctc
      121 tagcccctct cacaccaccg tacgtgccgt tcggcatacg gcagttcaac taacttttaa
      181 cgcatgtcgt tcaaggtaat aatccaaaca cgaaaccagt tcacgttttt caaggactgg
      241 ttttgatata gcacgtttaa gtaccgactt ctgagctact aattgatagt ggtcgcccca
      301 gccagatacc ttatccgcta tataggcctt attatcacgt tcctgttcgt agagccacga
      361 tttcgctatc ccttcctctg acgattctta tttccagcgt cagacttaaa acggtctatc
      421 cccagaccgt tttaatccgc tacctcacga tagtcagact tgggaatcgc tattgggttc
      481 accggtagca ggtgcccacc gaggacttac acctcaaata tataataaaa tgaaataaga
      541 acaggacgaa tcgatcagga cagtcaaatc aatttctaac aatgttttag aagtagatgt
      601 gtactattct agtttcaatc tactatacaa catgcccgtc gtacatcaaa aaaagttgag
      661 acatagcgtc ttcaactttt ttacattact ttaactactg attaaagtga gtttacgtca
      721 actttgatac caacaccttg agtagttgtg atagtcaagt ttgttacgta agttccttta
      781 gctgtagctg gttttgcttt ttggattgtt tcgttgaaag ctttaaagtt ttcaaccaat
      841 ttttcagctt caaatgatac tttaccgatg attgcttgaa cgttacctgc acggtcagca
      901 cggtaagtga ttttaccacc tttagactct tcaactgctt tagcaacatc cattgttaca
      961 gtaccagttt tagggtttgg catcaagtta cgtggtccaa ggacacgtcc aagacgtcca
     1021 acaagagcca tcatatcagg tgtagcgata actacgtcga agtccaacca accgtcgttg
     1081 attttagcaa caaggtcatc ttcaccaaca aagtctgcac cagcagcttt tgcttcttca
     1141 gcttttgcac cacgtgcgaa aacaagaaca cgtgaagttt taccagtacc gtttggcaat
     1201 accattgctc cacggatttg ttggtcagct tttttaacgt cgatgttcaa gttgtaagca
     1261 acttctacag ttgcgtcaaa ttttgcaaag ttagtttctt ttgcaagtgc tacagcttct
     1321 tctacactgt atgcttttgt gctgtcgatt ttctcaagag cagcacgaag ttgtttgctt
     1381 tttttagcca ttttctattc tccttgtaag tggttcaatc gattttcatc tcccacgtca
     1441 cttctcgaaa tgatgaagtc ttgcgggttt tcagtctact atttttctct tcctacggcc
     1501 ttaagctccc ttgctgtacc caagtacaag gctcagtcac ttgcctagta tgaaaagcaa
     1561 ctaatagact gctgtgagat tttctactgt gttattgatt agtcaacaac agtgaatccc
     1621 atagaacgag cagtaccttc gatcatacgc attgcagact ctacgtttgc tgcgttcaaa
     1681 tctggcatct tagtttctgc aatttcttgt acttgtgcac gagtaactgt agcaacttta
     1741 gttttattag gtgtacctga tcctttttca acacctgcag cttttttcaa aagaacagca
     1801 gctggtggtg ttttcgtaac gaaagtaaat gatttatctt cgtaaactga gataacaact
     1861 ggaatgatca taccagcttg gtcagctgta cgagcgttga actcttttgt gaatcccatg
     1921 atgttgatac cagcttgacc aagagcaggt ccaaccggtg gagctggtgt agctttacca
     1981 gcagggattt gcaattttac aagtttttcg acttttttag ccatttttaa atcctccttt
     2041 gtggttttgg cggtaattag agatttttac ctcccacaag tatgtttttc acatacccat
     2101 ccattataca ctatttatct ggaattgcaa gagaaaagtt tattttttgt actcaatgaa
     2161 aatcaaagag caaactagga agctagccgc aggctgctca aagtacagct ttgaggttgc
     2221 agataaaact gacgaagtca gctcaaaaca ctgttttgag gttgtgaata gaaccgacgc
     2281 agtcagtagg taaggcgacg ctgacgtggt ttgaagagat tttcgaagag tattaatcga
     2341 cgtagtctcc gccttcaaag ccatagtatt cttccaaact gagaccactt gcagcaattt
     2401 ctttagcttc ttttcctaca taacgcaggt gccattcttc agccatatag cctgtttcct
     2461 tttccttgcc tttgagataa cggacaacaa agccataatc agctgcatga tccaagagcc
     2521 attgggctgc tttttcttct gtcaccaaat caccatcagt cccaatcaca tcaaaggcca
     2581 agcctgtctg gtgttcgcta tagccaggac gggcagagta acggtcagct gctgcctttc
     2641 catcttggtt gacataatct tgatagagct tggtctgagt ttcataactt ctaaaaccac
     2701 tgtaatgatc actaataggg aaacctgcct cttgcatcgc tttgatgagt ttgaccaact
     2761 cagccttggc tgttggattt tccccgggat tatagtcttt cgacaaagga taatgtttat
     2821 tggctacgat gatttcgtcg tattttcctt ggatgctgta gtaatcacct ttattgacca
     2881 cttctgcttt tttctgggca gccccttgag acttactgcc gactgtccca tcagctttgg
     2941 ctgtctgttc tgtcttagct gtgccatctt cattttttgc tttttcttgt gaacaagctg
     3001 ctagactcaa ggctagcaag gctgtcaaga caaggtatct ttttttcatt tctactcctt
     3061 tatttctaat agtttatcta cttctgacga taaacattcg actaatcttt taatctcttc
     3121 cggttttact tggcaagttt ctgctgtcat ttcttcaaag ggaacccacc aaactggccc
     3181 acgaccttta agaccgtggc cattcatatc acctgtccgt cgtggaaaca gatgccaatg
     3241 aagatgggca tcgccatttc ctagcagttc gatattcatt ttctcagcag caaatgcctt
     3301 ggcaactgcc tcttggacta gactcatttc ttcgagaaaa cggagtcttg tttccttttt
     3361 caaatggtgc aattcggtaa cgtgttcctt agctagaaaa agactatatc ctgcaaaata
     3421 ctggtggtct ccaatcacaa gatagcctgt ttccaactct ttgacaaagt aaggattttc
     3481 ttccttcttg atgaggtcaa ttctctggca aatcaggcac atattagtct accaacccac
     3541 tcttaagata agcatcaacc atacgctctg ttgcgaccac cgagtcaata tgagtacgct
     3601 cataagaatg actagactcg atacccgcac caagaagagc gtgtttaact tctgcacctg
     3661 cagacatagc cgctgaagcg tccgaaccat aaaatggata gatatccagc ttgaatggaa
     3721 tatcttggtt tttagccaag gtcaccaaat gttgacggaa gtcatagtga taaggacctg
     3781 aagcatcctt gacacagatg gacactgtgt actcatctgt ttgctgatca tcccccatag
     3841 ctcccatatc cacagccaga tattcgacta cttgagcagg aatattagaa ttagcaccat
     3901 gccccacttc ttcaaagact gaaaaggcaa aatgggttgt tactggcaac tcaatcttct
     3961 cttccatata aatacgaagc aggttaagca aaatcgctgc gctgaccttg tcatccaaat
     4021 gacgagactt gataaaacct gtatcagtca caacagttcg tggatcgaag ctgataaaat
     4081 caccgacctc aatcccaaga gcacgagttt ctttttcatt gcttactttt acatccaaac
     4141 gcacttccat attgtcctgc gtgcgttctg cagttcctgc atccttgtag acatggcaag
     4201 aagtttggtg gatgaggatg gtgcctgata ctttttcacc tgtgctagcc acatgaacgg
     4261 tacagttttc accttcaatc atgttccaag gaaaaccacc gatacggtcc aatttaagac
     4321 gaccatctgg tttgacagca cgaacaatag cacccagcgt atccacatga gcagtcacat
     4381 agcgatgttg ctcatcattt tgacctttga tggtcacatt gacaccaccc ttagctgtgc
     4441 gaactggctg gtaaccaaaa ccttctagag tcttgactaa ataattcgaa atttcacgag
     4501 taaagcccgt tggcgatgcg atggctgtca gttctttgat atattctact gtttgattca
     4561 tttcttcacc tctttcgcta cctattataa cacatttatc atcggataaa aaaagaaaat
     4621 cactcctgta gacttggcta caaaagtgat ttaagtgatt attccatttc aaaaacatcg
     4681 ctttcttgct tgtttggtag aaccaatgag agcaagattc caacaatggc tggcaccaac
     4741 catgggagag ataccttggc aaaaggaaga acgctaacaa gatttgcaag aaactcaacc
     4801 ttaaacgagc ttcctagtac gcttgcaatg gcaataactg taaccacagc aattgtcaac
     4861 tgcatacctg gttttgaaag ggcaacaaat ttgttgacaa tgacaatcat aacgatagca
     4921 atcgtgattg ggtacaagat aaccaatact ggaattgagt acttgataat cgcatcaaga
     4981 cccaaattgg caatagcaaa tccaatcaag gtaaaggctg tcgcataaac cttgtagctg
     5041 atttgtggga agcgttcatt aaagaactca gctgttgaca caatcaaacc aacagtcgtt
     5101 gtgaagcagg ttacggtaac catagctgca aggaagagtt gagctgttga gccaaagatt
     5161 tcttgagtgg cttgtgacaa gatgtaaaca cctggtgttc cacccttcat cgcttcagct
     5221 ggtactggga aatgatttcc aagaaaacct aaaccgatgt aaagagcgct gaaggcaagg
     5281 gcaacaacga taccaacaac ccaaatagtt gaaatgtatt ctttcttact tgaaaatcca
     5341 agttgtttca aggtttgaac tgcgattacg ctaaaggcca ctgaggcaag ggcgtccaag
     5401 gtattataac cttctaggaa acctgtacca aaggcagaag cttgataagc agctgaagca
     5461 gcttgaggac ttgttccacc atatttgata gctcccagaa cgaccaagat aacaatcaaa
     5521 attgcaaaga ctggcgttaa aatacgtcca atgcggtcta agatttttga tggattaagc
     5581 gaaatcaaat aggctgccgc aaaatacaga accgtaaata caatcaagcc aagtccttta
     5641 tttgcatccg acaaaagggg gctaatccct acttcgtaag ctgttgtagc agtacgtggg
     5701 gtagcaaaga atggaccgat tgacaagtaa agaactgaga ggtaaagagt cgcaaaccaa
     5761 ggcgctatct tcgttgaaat ctcgtagata tatcctttag gatttagcgt tccaataata
     5821 agggtcaaga cggcgatacc aacgcctgaa aagacaaaac ctgcgatggc aggaagaaaa
     5881 tgttctccag atagagcacc tagagaaggc ggaaaaatca agttccccgc accaaaaaat
     5941 attccaaaca ggagcaaacc tgttagggta ccttttttag ccataaaaat ctccttcata
     6001 tttataaaat actgacctag tataccacga ttaggagtct gaaaaaagca tcacgaggca
     6061 aatattaaat gttttcaatt ttcttaaaaa taagaattat ctaaaaataa aactctccta
     6121 cccagtccac accaagtagg agaaatgcaa aattttaaag ttcttcaaag gctaacatgc
     6181 caccctggac attgataaca ttataacctt gttctaacag gaattggcaa gcacgcgccg
     6241 atctcattcc agatttgcaa ataatataat gcaagcgatc tttgtccaat taatcataac
     6301 tatcagccaa ttgactaagc ggtaggttgt gggcacattc taaatgaaga gctgcaaact
     6361 catccacttc tctcacatcc actagagaaa gctggtcgtt ttggtaaagc tggtaaaatg
     6421 cgtcaaaggc tatttctttc attctttttc ttctaatact cttcgaaaat ctcttcaaac
     6481 cacgtcagcg tcgccttacc gtgcgtatgt taccgacttc gtcagtttta tctgcaacct
     6541 caaagctgta ctttgagcag cctacgacta gcttcctagt ttgctctttg attttcattg
     6601 actataaaat ggttttaatt ctttttttca aatctggcac tacttctgcc tcaaaccaag
     6661 gatttttggc catccagatt tgatttcgtg gtgatgggtg aactagcgga aaataggctg
     6721 gcagatagtc tttatagtgt ttcaccctct ccgttacctt cccactgatt ttctcctgta
     6781 aatagtaggc ttgggcatat tgcccaatca agagggttaa ctgaatatca ggcaattcct
     6841 gtaagagctg cggatgccat ttttctgcaa aacctgtacg aggcggaaga tcacccgact
     6901 tgccatgtcc tggaaagtag aaatccatag gcaaaacagc aaaataacct gaattgtaaa
     6961 aggtatcttc atccacacct agccagtccc gcaagcggtc accactttta tctttccagt
     7021 aaaggcctgc ttcttgagtt ttaagtcccg gagcctgacc gatgatattg atgcgagcag
     7081 tttttggcgc tgcaaagaga ggctcaatgc cacgctctgt atagctggca ttctgtgaat
     7141 ccgccatgat agcctgtttg attctttcga tttgagacat ctactttccc tccaaaaaga
     7201 agtccaagca taagatctca gacttctatc gttttatttg atcaattcgt aaatagcttc
     7261 ggcataaatt gctgctgctc ggaaaagatc atccaaggcg ataaattcat tggcttggtg
     7321 catggtatca atcgagtctg ggaacatagc accgtaggca actccgcgtt ctagcaagcg
     7381 accaaaggtt ccaccaccga tgacttgttc atgaccttta aggccagttt gtttttcata
     7441 gatattcaac aaggtttgca caagtggatc ttccattggc acatagtgag gcgtgtgacc
     7501 gtgttcagac agactaacag aaacaactgg caagttttca aggattgact tgatttgttc
     7561 tggacttgtt ccttttggat agcggatgtt gagggcaatg gtattatcag cacttgtttc
     7621 atcgaagcgg aagacgccgg cattcataga aagagcaccc atcttttcat ccacatgagc
     7681 aatcttaaga ttttcaccct catgatcgtt caagagaatt ttacctgcga tgtcaaggta
     7741 gtctttggct ggaccagcaa agccaaactg gctgaggaag agggcaaggt aagttgcgcc
     7801 attgacacct gaagcaggca tagcaccgtg ggctgattta ccaatgatcg tcaccttgta
     7861 tttgccagct tcttcttgga gttctcctct aagtttgtgt tctgcaacaa aggcatctag
     7921 tttagcttgc aagtcagcca aatcacctga aacgactgct gttgctgatt ctggtaccat
     7981 attttcacgt aaaccacctg taaagctgtg aagacgggca gcacctgtat tttctcctgc
     8041 aaagtggagg tattccgtga tatttccttt ttcaccattg atgattggaa attcagcatc
     8101 tggagagaaa ccgaaatctg gtttggcaag tcctacgtgc tcaaagtagt agtccatgtc
     8161 tgcccagcct gattcttcgt ctgttccaac gatgaagcga actttcttag aagttggaag
     8221 acccaattct ttgatgattt tcaaaccata gtaacaagct gttgtaggac ccttatcgtc
     8281 cgaagccccg cgcgcataaa ggcgaccatc tttgatagtt ggtgtgtaag gatctgtgtc
     8341 ccaaccgcta ccagcaggca ccacatccat atgggcaaag attccgagaa cttcttctcc
     8401 atcaccaaac tcaaaatgtc ctgcgtagtt atcgacattc ttagttgggt agccatcgcg
     8461 gtctgcgatt tcaaggaatt tctccaaggc ttttactgga ccaggtccaa atggatgctg
     8521 ggcatcagcc ttgctatcat cacgttctga gttgatttcc aaaaggctaa acaagtcagc
     8581 caagaggtct tctttgcgtt tttctacttc tgctgtaaaa tcaattgctg tcattttttc
     8641 ttctttctat cttttctcga tgatttcatc tactggcaag cggtagcttg gttccaattt
     8701 gtcgtctgtg taacccactg tgatcaagag ttctgggcgg aaacggtctt cgatttccaa
     8761 aacttcatta acttttgatt tgtcaaaacc aaggataata ttggaaccaa ttccttggtc
     8821 tgtaagagcg agaaccaagt tcatagcaac caaacctgca ttgagtgcta ggtagtcgct
     8881 gacttgttgt tcattgtagc gtgcaaattc agctggcaaa tttttcatga agtattgaag
     8941 ttgttcttct gagaaattgt tagcaccacc aactcgggca atcttacgag cgcgtttggc
     9001 aaggtctgta tctgtaaata gggcaatggt tacaggtgct gatgatacct gctcaaagtt
     9061 tgaaccataa gccaactttg ccagttcagc atttttctca cgaacaacta caaatttcca
     9121 aggctggctg ttgtgggcgc ttggcgccaa ggttgcaatt tcgatagccg tacgcacatc
     9181 ttttggatca acaggcttat cagtgaaatg cttggtcgca tgacgttttt tatttaattc
     9241 aagaaatttc ataatcgatt tccttttcta attctactgc ttccattcta ccataatttg
     9301 aaagagcttg cagggatttt tcatgattaa gtttttctat cacagacata aaagatatat
     9361 attggtttat caggaaagtt tctgataaac tagcaagtac tttacatttg aatggagaca
     9421 gtatgaaaaa atttagccta ttactagcta tcctaccatt tttggttgcc tgtgggaatc
     9481 aagctacacc caaagagtcc agctctcaaa agacaatcgt ccttgctaca gctggcgatg
     9541 tgccaccatt tgactacgaa gacaagggca atctgacagg cttcgatatc gaagtcctaa
     9601 aagcagtgga tgaaaaactc agcgattacg agattcaatt ccaaagaact gcttgggaga
     9661 gcatcttccc aggacttgat tctggtcact atcaagcagc agctaataat ttgagttaca
     9721 caaaagagcg cgctgaaaaa tacctctact cgcttccaat ttccaacaat cccctcgtcc
     9781 ttgtcagcaa caagaaaaat cctttgactt atcttgacca gatcgctggt aaaacaacac
     9841 aagaggatac cggaacttct aacgctcaat tcatcaataa ctggaatcag aaacacactg
     9901 ataatcccgc tacaattgat ttttctggtg aggatattgg taaacgaatc ctagaccttg
     9961 ctaacggaga gtttgatttc ctagtttttg acaaggtatc cgttcaaaag attatcaagg
    10021 accgtggttt agacctctca gtcgttgatt taccttctac agatagcccc agcaactata
    10081 tcgttttctc aagtgaccaa aaagagttta aagagaaatt tgataaagcg ctcaaagaac
    10141 tctatcaaga cggaaccctt gaaaaactca gcaataccta tctaggtggt tcttacctcc
    10201 cagatcaatc tcagttacaa taacactctt cgaaaatctc ttcaaaccac gtcagcttta
    10261 tctgcaacct caaagctgtg ctttgagcaa cctacggcta gcttcctagt ttgctctttg
    10321 attttcattg agtataagat atactaaaag cgattggacc agccaatcgc ttttttagtt
    10381 tgcattggct attttaggaa actcttacaa aaaatcaaaa aaattcttca catcctctac
    10441 atacccatgc ttttctatta agctggctaa taattccaga ttgtgcccct gatagttatc
    10501 gtcacgacgc agttcttcat acatttcgat aaagggaagc cccttggact tggccaattc
    10561 taactcagct tcgtaatcat aagcaacttt acgaaattgg atattgagta attccccatc
    10621 ttcaacttct atcacggcat actgggcacg gtgatttttt aacgcctccc aattaaaata
    10681 gggcatgcca atcgaccctg gattgatgat ttgttgccct tgacttccat aacgaagcaa
    10741 ctgcttgtga acatgaccat aaactgccac gtccgtttcc gcatctagca gttggtcaaa
    10801 tttctctgta tcattctcaa ctagcaagtc accaccatag tttttgtcag gtaaattatg
    10861 agagatagaa aagcgcaatc cgtcaatttc tttcttttcc agcaaaggca agcttcgtag
    10921 ccagacaatc gttgcaggat ccattcgctc catcaaatac tgtgtcatac gcaagagctg
    10981 aacttcctgt gggtcttcta agccatattg cccatctaaa gcctcaagga cacgatcatc
    11041 ccaattgcct cgaacacttg ctgtgatagg aaggtccttt agcagggcga ctaagtcatt
    11101 tgcgcctgga ccaggaagaa aaatatctcc cagaagccaa tattcactgg ccccttgatt
    11161 tttagcatct gcaatcactg cttctagcgc cgtcgcattg ccatgaacat ctgataaaat
    11221 tgcgatttta tggttcattg tggtttcctt ccgtttggta atctactctt tcttctgcta
    11281 cttgaggcaa ggcttctccc tgcgggttca actttctttg cacagcctct gcaacgactc
    11341 taggtacccc aacttcgaca atctcatcca cactggcttc cttgattttg gtcaaagact
    11401 tgaaatgcct catgagattt tgcttgcgtt taggtcccag accatcaatc ccatccaatt
    11461 gagatgagaa agaatttttg gagcgcagtt ggcggtggaa agtgatagca aagcggtgga
    11521 cctcatcttg gatgcgttgg aggaggaaaa attcctgaga attgcgagac aagtccacca
    11581 cctcaagcgg atctccaaag agcaattcat gggtttggtg cttatcattc ttttgcagcc
    11641 cagcaattgg aatatccaag cccagttcct cttggataac ctgcttagcg atattgactt
    11701 gaccttgccc cccatcaatc acaatcaaat ctggaggagt caaagcctca cgctgtactc
    11761 gaccatagcg tctgcgaatg acctctctca tgctggcata gtcgtctggt ccaacaaccg
    11821 tttttatctt gtacttacgg taatccttct tactcggttt accgttgaca aagaccacca
    11881 tagccgaaac agggctagtt cccatgatat tagagttatc gaaggactcg atacgtactg
    11941 gggtcgggat ttggagcaaa cgccctagat tttcaatagc tccttgggtc ttttcgacag
    12001 atttttctag cagattgaac ttctgctcta gactaacacg agcatttttt atggctagat
    12061 tgaccagttg ttttttctct ccacgttgag gcttaagaat cttggaatcc accaaggcct
    12121 tgacagcctc ttcgtcaata tcctgcggaa tcagtacctc attgggaact agatgagatt
    12181 tttcttgata gaattgtcct acataggtca aaaaatcctc atctggatca ttgaagtagg
    12241 ggaagagatt gacatcgcgc tcgataagct taccctgacg gacaaagaaa acctgcacac
    12301 acatccagcc cttatccaca tagtagccaa agacatcgcg attttgcaaa tctttcgcca
    12361 tgacccgttg cttggttcga agcgttccaa tagcctgaat caggtcacgg tattccgccg
    12421 cacgttcaaa ctccatactt tgtgctgcta ctgccatttt actcttgaga tcatcgatga
    12481 ttttgtcatc ctgacctttc agaaaatcag acacctcctg agccatagac ttgaaataag
    12541 cctcatcctt cttacagatg gtgtgggcca tacactggcc gatatggtaa taaaaacaga
    12601 ccttagaggg cgggttggta cattttcgaa aagggaaaat cctatctaga agtcgcttga
    12661 tttcattggc tgcccccaca tctggataag ggccaaaata aagaccaccg tcctttttga
    12721 cctgacgagt aatgataagc cgtggatagc gctcattggt gattttgatg aaaggatagg
    12781 acttgtcatc cttgagcatg atattgtact tgggcttgtt ttccttgatc aggttgattt
    12841 ctaggagaag tgcctcaata ttagactccg taacaataaa ttcaaaatcc acaatttcag
    12901 acaccagagc ctctgtcttg gtatcatgac ttccacgaaa ataggaccgt actcgattac
    12961 gcagattttt agcctttcct acatagataa tggtgccatt tttatcctta tgaatgtagc
    13021 aaccagggct ggtcggcaag agctctagtt ttgatttaat caagttgttc atagttccat
    13081 tatagcaaaa aaagtaggga aagatagtac cctactcact agtctcatat atttttcgga
    13141 ggtttgcaac atcctcttcc tggataccat aaaaggaacc tgctggttgc atgacagact
    13201 tctcatctgt atggtccaag ccaatcgcat gacctaactc atgttctgcc gtatggacaa
    13261 ggcgttcata ggagtagcca tagtatggat tggacaaata ataatgattc aaccgcaccg
    13321 ttacggacaa gaattgccct gttaagagat tagtttgact ttccgcctct cctgccacag
    13381 gagtgcctcc gtcgttcatc tccgtagccg taatatccgc cttactagac tcagtcacga
    13441 gttcaaagtt aaaagcacca gtttgattcc agttctggat tgcttctaaa taagcctctt
    13501 gaaagcgtga atccatctgc ggatccaggt aaatacgagc agaggcctgt gcccaccttc
    13561 cttcagaatg ctggtgacta tctgtctggc ttaacttagg cagttgccct gttttctccc
    13621 attggtcaat actttgttga ccaattttta ctgaccgttc tgcttgcttt agcgctcctt
    13681 gaaaatcccc gttcagatac cagagaagtc cgaaagcaag cacacataag agcacaacta
    13741 tccaaaccag acgccaaaac aaacgccaca caaaagaaaa gaaagcccct atcaaacgaa
    13801 aaagccagcg catgtctctc cacctttcta gcaaataaga attattttac taaaagaagc
    13861 taaaagaaat ctaaatactg actttttcat cttaacgtcc tactattttt ttgagtaact
    13921 ggatagcttt gtattttaaa ggcgatggat ggtaacggaa agtacccgct ttgcgtacaa
    13981 tatagccatt aaaattctgt ttaaaacgca aaacaccatc acttccatca aaaatccctt
    14041 gaatgcctag gaagttgtat ttaggtattc cacgttttat gctttccaac ataacatatt
    14101 tttgaagcag ggcaggggca tagaacttat taaactcagt gtaggaacca ctaaagagat
    14161 aagtcgtttc ctgaggcata taaacaaata aactcccagc taaaacaata tcttcttctc
    14221 catatttttc aatcaagtct cgcgcttctg cttttcgaac ttcaaacgtt tcaaattgac
    14281 tagaatattc tctcagttga ttttgttttt tctcagaatg aggattttta ctcaaatcaa
    14341 gtcgcaactt gtccaagttt tcttctagtt tactttgttc accttgcaat ttgctcatat
    14401 agtccgaaaa atttaagctt gctatgagaa actccgcttg ttctccaaaa gtatcataaa
    14461 aatgctcata atattctaaa cttttatcac tatattctct acgttcagag gtttcttttg
    14521 ttatattctt aaaaatcgat agttcttcac gttttaactt tttcaaccga atgccaaagg
    14581 tttcagcctt tttcaccaag ggtttaccct ttttgctaaa acttttaagc aaactctttt
    14641 cagttaattc agttaaatct ttatagtata accaatctgg ttctccacct gggtaacctg
    14701 ttgttaagcc atcaaattga taacctaaat cagtcaaacc ttgaataata ctttttttct
    14761 cagcatctat tgaattacct tggctatcaa aagtttgata agtttcataa ggttttacaa
    14821 gcaactctaa tacaccattt tgcttggcat attcttttaa ctctgcataa aaaactggaa
    14881 gagcatcttg ttgggtataa atcggccccg aattgagttc catatgcaga ccacccagca
    14941 tgggcaagct ataaaccaga gctgcaactt gaatttctcc ttcttgtttc aaagcaagat
    15001 aaacaattcg agcccctctt ttttctagca aatcccccat ctggacagat tgcataaagg
    15061 aacgagaaga aacctgatca gaataagtct gaaactcttc tttcgtgagt gttgttagtg
    15121 ccatatactt actttctatg tttttttctt aatgttttac ggaaatcaag agcaagtctt
    15181 aacagaggat agagaggatg agtgggcatt gtaaattcac ccaagtattc ttcaatcgtt
    15241 ggattaaatt tttccttaaa atgataaagt ccaccattga gagagttttc aacaccacct
    15301 aaattttgcc aaaccatacc tcgctcaaag gcatagcgag ccgtttcata ccatgttaaa
    15361 attggtgcat tgtaacgttt aaaatcatca tccataccag catatatatt gacagaggta
    15421 gtaccaaatt ccaaactcaa agtagccgct aaaggaactc tcgcttgacc tacatctata
    15481 tattcctgca agaaggtcaa ttcctctaac aaacgttctt tttccttctt ctgcgcttct
    15541 acttttgaaa ttcgagtcga ctcagtaaac gtctcttcca aggctctatt tttcgctaac
    15601 tgttcttcta attcttgcga acgtttagaa acatccaagg tggccaaggt gatataggcc
    15661 ttgtccttaa aattatctaa caatttttta taataggctt cattcctcaa atgaatctct
    15721 tttcgcttct cagttttttt catcaactcc gaaaatgaat ctaatagttc cagtccacca
    15781 tattgaatct caagctcttt gtttcgtgct gttcgaatag cctgttttgt tgacttggaa
    15841 agtttatctt cttcaaaatt ttccttgtat attttcgcct gaatacgagg ttgaatggtg
    15901 tctcccattt cctccgtttt tcctgaccac cttactccca tttgttgcaa actatcaata
    15961 atagccagat tttcaggaaa ttctgtcttt tcctgattga ttaaactttg agataggcaa
    16021 atacttgggt caaaagtcac aaaaaccgct ctcttactgc gagcatagga cttaatagac
    16081 tgaatgacaa aattcaagag ttctttatcc ccataatcca atataggtcc tcttgggatg
    16141 taaaacattt tatagcctag cggaagagtt ctaatcaaaa tactagctgt cgccagtaat
    16201 ttttcttccc tgtaaacacc aaacttctca tgttgccaat tagacttaac ttcctcccaa
    16261 gcactacttt gtaatacatt ggctaattca tgttctttga caaactgatc atattctaat
    16321 gtgggaatgc caatttgata acggtacatt tcctactctc tttcctccag tattttatta
    16381 ctattctact actttcgttt gaaaagtcta gtgaaaacat attttatttt agttctactc
    16441 cccctatgct gaagttcaaa ctaagtcagt ctctcttcta atttgaaatc aattggtagg
    16501 gttgttaaaa aaacttccaa ttgtttttcc cagtagtacc actcgtgagt tccagcgcta
    16561 tggctatagg tcacatcaaa acctagtttt ttgagatttt tcactgcgag attattggct
    16621 tcgtacaaga aatcctgttc gccacaccac gcccaaagtt tggtcttttt atccgatttt
    16681 ttagccagac tttcaagaga atagggacta gttgtccagt ctttaatctc tccaaaaaca
    16741 cctctccagt aggctggact tcccagattt tgactttcag gagaaaagtt ttgaaagctg
    16801 agggcacctg aaaagctagc tgcatgagaa aaacgatttg tcgcaagagc cagtttgaag
    16861 cagccgtagc ctcccataga aagaccagcg ataaaggtct tttcacgctt gctagtcata
    16921 ttagggaaga agcgtttcag aacctgtggc aattcctctg ctagagccgt gtagtagtca
    16981 aaaccatact gggtatcggt gtaccaacca ttgctggtat tgggcataac aacgatgaga
    17041 ttagttcctc gaagcaagcg ttctacattg gtccgcttaa gccaactatt atgatttcca
    17101 gacatcccgt gcaaaaggta caagacggga atatcttcac actctggttc ttccactcga
    17161 ttggcatcag ggtagaggac attcaccccc cactccatat ccaatacttg tgagtaatac
    17221 tcgattttca ttactgccat gattttctcc ttctattttt ctataagaaa aagccgaaac
    17281 tcgactttct ctctgtttat ttcaacgatt attttgcttc catgactact ggtaaaatag
    17341 ctggacgacg cttggtttgg tcaaagagat acttggtcaa attatctcga accttccctt
    17401 taagatctgc ccagtcaaag tcatctcctt gaagatactc ttctaccgtt tggttaatca
    17461 attctgaact ttcacggaga atatcgcgac tcttcttgag ataaacaaat ccgcgcgtat
    17521 gaacacgagc cctagccaca attttcttct cacgacggtt gactgtaata gccacgatga
    17581 aaattccatc ctctgacaag accttacggt cacgaagaac aacatttcca acatcaccaa
    17641 tggcattccc atcaatcaag atatctcctg ctgaaaccga tccagctgga acaaagtctc
    17701 cattctcgta agccatggtc gtcccctttt taggaatgaa gatgtgttct ggcaacatcc
    17761 caactgccat ggcagcctta gcgtgagcat ccaactcacg atactcccct tggacaggga
    17821 agaggtactt aggttgcaaa agattgatca tcagctgcaa atcacgcaca tttccgtgcc
    17881 ctgatacatg taaactttgg gtaatcaatt tgacaacccc acctgcctga taaatcatat
    17941 tttccacacg cgcaacaaag gcttctttag caatagacgg agccgtagca atatagacca
    18001 ggtccccatc cttgatttct acataacgat ggcgaccaat cgacatctta cgaagtccat
    18061 tgataggttc acccatacga cctgtctcaa gaataatcaa ctcatggtct tcaaagcgag
    18121 acatatcttt aggcttaatc aagagaattt cgttggctaa agacaacttc ttaagacgaa
    18181 tcgctgtgcg gacgatattt tcaatatcaa atcctgtcaa gacgatacgt cgacctgttt
    18241 tatccgcagc gtcaaaaatc tgctgaatac gagaaagatt actggaaaca gctgcaacga
    18301 tgatacgacc ttcccagtca gcaatagttt gggtaatttc atccctaact tcactttcac
    18361 tagccacctg aatattgctg tctgcattgg ccgaatcact gaggagagcc aggacgccgt
    18421 cacgaccaat ctctgccaaa cgagcaaaat cagttgcata agattcacta gccgtttggt
    18481 caaatttgaa gtcacctgta taaacgatgc ttccttccga tgtcttcaag acaattccca
    18541 gactctctgg aacggagtaa gtcgtaggga agaaggaaac cactgtccca ccaaaatcaa
    18601 tctccgtatt ctcatcaatg acatggaaat cattaaattt cttaacggca tcatttcctt
    18661 tgacaaagag ctttgccaac tcaatggtca actcagaccc aaatacagga actttagcct
    18721 ctgccaagag ataaggtaga gcaccaatgg catccgcatg cccgtgggtc aagaaaaccc
    18781 cagcaatacg gtcgctattt tcaaaaaggt aatccatgtt tggaatcacc acatcgaccc
    18841 ctaattgttc attttcagga tattttaacc ctacattcaa aacaaaaatg gactctccaa
    18901 tttcagcaat gtacatattt tttccattct cacgcacacc accaagtgtt gttaaactga
    18961 tattactcat ttttcctcca aaatcttagt ttatcttgat tatagggcaa ctctaccctc
    19021 ttattctaaa agttctaata ctttcagccg aatcttttcc taccattata ccattttttt
    19081 gatgattttc aaaatgaagg tctgttttca aaaaagagtt gatttcccaa ctctttccta
    19141 acttctactc tttttccatc aaaaaatcat aaatttcttg caccgtaccc agcgccatca
    19201 tttcttggtc tttaacggtt tgtttgagat tttggtaaat ttgactttct cctatctccc
    19261 gctttggcgc ctctttattg actgtcatca caccatcttt tatcgtcaca aagcctagtt
    19321 cttcaaatac ttgaatcatc ttgaccagca agatttgttg aatattaaga tatgcagcca
    19381 aatctttcag cttgtagcga atatcaaact ctgggaactg gtaaatagtc ttgtacaatt
    19441 tggcaaactg atctctagtc ccataacctg tcagataata ggccttgtca atatcatttt
    19501 tgaaatagac agcagagaaa tgctgttcct gaaaaatggt cttcagctga gtaatatcct
    19561 ctggaatgtt ttttacgaca atagcttcac tagccgcaag atttggcagt tctccaggaa
    19621 aatccaagac tggaacacct tctggcaaga ctgcattttt tccacgaatg ttaaaaagtt
    19681 gaacaccttc cactcgcgca tccaccatca tcaactggag ggcagtttgg ccattccatt
    19741 ggttgacaga caatttaacc gctaactcta gattcttggt ttgagaaaac tctgtcgccc
    19801 atctgccttg accaaaggct accacttcaa aactcgcccc acccttggaa attttcagct
    19861 ttagatgggc attacctgcc cccatagtac gagcactttc gacctgaaaa ttcttgatat
    19921 aaaaaatagg tttctgatta tccattccaa aaggagctaa acgttcaaaa cttttgaccg
    19981 tttccaagct aagtgcctcc aaatccaact cttcatctag gtttaactta ttcttgccac
    20041 cagcatctgc acctttttca cgaacataat cttccaaaac ctgagataaa tctgagagtt
    20101 gctcaacttc cagcgtcata cccgctgcac ctgcatgacc tccaaaggcg atgaagaggt
    20161 ctcgatgggg atccagagct tcaaaaatat cgaccgcttc cacactacga gcactgccct
    20221 tggcacgacc gtcttctata ttaagaacaa tgactgtctg tcccaattct tccaataaac
    20281 gaccagccac gattcctaga accccaggat tccagccttc cttggccaag acctgaacct
    20341 tcttctcagg atccaccatg gtcttggctt cttcatagat agactgaacg atttccttgc
    20401 gctcttcgtt tttctggtga atcataaggg caatctcatg cgcttcctca tcatcaaatc
    20461 cagtcaacaa atcaatggca ggattgggat catccaagcg acccaaggca ttcaaacgag
    20521 gagcaatctg gaaaccaacc gtttcttctg ttacttcgtt ggcagcaatc ccagccatgt
    20581 ccagcatttc ttgcagacca atgcgctggg tatgacccaa catttccaga ccatattgaa
    20641 ctaagatacg attttcatcc gtcagactca ccatatctgc aatagttcca atagcgacca
    20701 aatcaagcaa ttccacttgc acttcttcta acagggcaca agccaacttg aaagcaactc
    20761 cacaaccagc caaatattta aaaggataat ccgcatctgg atgttcagga tggacaatag
    20821 cataagcatc tggcagggtt tcaggcatgg aatgatggtc tgtcacaatg acatctactc
    20881 ccatagactg agccaatgca atagcctcat gaccagcaac cccattgtcc accgtcacaa
    20941 tcaaggaaat tccttcttgc tcgataaagt atttataaac actagcatta gggccatagc
    21001 catcggtaaa acgatttggc aggtaaactc ggcactccgc accaagttgt tccaaacttt
    21061 ccttcacaat agaagccgaa gtcatgccat ccgcatcgta gtctccataa acgagaatat
    21121 tttccctttc ttcaatagcc tgacgaatcc gctccactgc cttgtccata tcatggagca
    21181 gataagcatc atgtaagtcc tccaaggaag gttctaaaaa cttcttcaga ctttcttggt
    21241 cctgaatccc tctctcaaac aataaccgag ccacctcagg acccagtcca gccttcttgg
    21301 ctatctttgt aaaatccgca tcttctacct gcagggcaaa ctgccattca taagtaggtg
    21361 ttatcaaaaa gacatccact ccttctaaga attctattgc ttcattataa catgatttag
    21421 acgttttctc tatccagatt gcttttttat aacatttcat agtatattga aactagaata
    21481 gtacacctct acttctaaaa tattgttaga aatcgatttg actgtcccga tcgatttttc
    21541 ctgttcttat ttcactatag tgaatttttt cagatatggt ttgacaagaa cattctttta
    21601 ttgcagaatt gtgttataaa atctaacata aaggagttct ctcatggctt tagtagaatt
    21661 taaaaacgtc gaaaaatatt acggagacta ccacgcactc cgcaacatca atctccgttt
    21721 tgaaaaagga caagttgttg tcctgcttgg accttctggc tctgggaagt ccactcttat
    21781 ccgtacgatc aatggtttag aggctgttga caaaggaagt ctcctagtca atgggcacca
    21841 agttgctggt gccagccaga aagatttggt acctcttcgc aaggaagtcg gcatggtttt
    21901 tcaacatttt aacctttatc cacacaaaac agtgttagaa aacgtgacac ttgcacccat
    21961 taaagttcta ggaattgata aaaaagaagc tgaaaaaacc gccaaaaaat atctggaatt
    22021 tgtaaatatg tgggacaaga aagattccta tcccgccatg ctatctggtg gacaaaaaca
    22081 gcggatcgcc atcgctcgtg gtcttgctat gcatccggaa ctcctcctct ttgatgaacc
    22141 aacatctgct cttgatcctg agactatcgg agatgttcta gcagttatgc agaaactggc
    22201 gcgtgatggg atgaacatga tcatcgttac ccacgaaatg ggctttgctc gagaggttgc
    22261 ggaccgcatt atctttatgg ccgacggaga agttttagta gatacgacag atgtcgataa
    22321 cttttttgac aatccaagcg aacctcgtgc ccaacaattc ctcagcaaaa ttatcaacca
    22381 cgaaagtgac aaagtcaaat aaggaggcgc ctatgaaaaa aaaattcttt ttatcagcat
    22441 tattgattag ccttttcggc cttgctgctg ccaaaccagt ccaggctgat acaagtatcg
    22501 cagacattca aaaaagaggc gaactggttg tcggtgtcaa acaagacgtt cccaattttg
    22561 gttacaaaga tcccaagacc ggtacttatt ctggtatcga aaccgacttg gccaagatgg
    22621 tagctgatga actcaaggtc aagattcgct atgtgccggt tacagcacaa acccgcggcc
    22681 ccctcctaga caatgaacag ggcgatatgg atatcgcgac ctttaccatc acggacgaac
    22741 gcaaaaaact ctacaacttt accagtccct actacacaga cgcttctgga tttttggtca
    22801 ataaatctgc caaaatcaaa aagattgagg acctaaacgg caaaaccatc ggagtcgccc
    22861 aaggttctat cacccaacgc ctgattactg aactgggtaa aaagaaaggt ctgaagttta
    22921 aattcgtcga acttggttcc tacccagaat tgattacttc ccttcacgct catcgtatcg
    22981 atgccttttc cgttgaccgc tctattctat ctggctacac tagtaaacgg acagcactac
    23041 tagatgatag tttcaagcca tctgactacg gtattgttac caagaaatca aatacagagt
    23101 tcaacgacta tcttgataac ttggttacta aatggagcaa ggatggtagt ttgcagaaac
    23161 tttatgatcg ttacaagctc aaaccatcta gccatactgc agattaagga ggacacccca
    23221 tgacagattt atcatcttgg acagcctatt ttcaggattt tggacaattt ttcaatggtt
    23281 tcctcttcac ccttgcccta gcgattggat cctttatcct cgccatggtc ttaggaatct
    23341 tctttggtgc catgtcaacc agtaaacgtc caattttacg cattttagct cgcatctttg
    23401 tggaatttta ccaaaacact cccctcttgg tgcagtttgt tattgttttt tatggcctac
    23461 ctcttatcag tgaccatact atcatgattc cgatttattg gacagctgtt ctctgcgtgg
    23521 gactctatca cggcgcttat atcgctgagg ttattcgttc agggattcag tctattccta
    23581 gtggtcagat ggaggccgcc ttatcgcaag gttttaccta tatcagtgcc atgcgcttga
    23641 ttatcttgcc tcagaccttc cgcatcattc tccctccatt gaccaaccaa attgttaacc
    23701 tcatcaagaa cacctctact gtagctatca tctctggagt agacttgatg tttgtgacta
    23761 agtcttggtc ggctctcaac ggaaactata tcccagcctt tttaggtgct gctcttctct
    23821 actttgccct atgcttccct gttgcccagt ttggtcgcaa gatggagcaa gccaacaaaa
    23881 aagcctattc actttaggag gttactatgg aatctatttt agaagtttta accccagata
    23941 acctagtctt tatctttaaa ggatttggct tgaccctcta tatttctctg attgccatca
    24001 tcctctctac tatcatcggt acggtgctag ctgtcatgag aaatggcaaa aattctgtct
    24061 tgcgaatcat ctccagtatt tatatcgagt ttgtgcgcaa cgttcccaac cttctctgga
    24121 tttttactat ctttttggtg ttcaaaatga aatccacacc agcaggtatt acagccttta
    24181 ctctctttac atcagcagcc ttggctgaga ttattcgagg cggtctcaat gccgtagaca
    24241 agggacaata cgaagcagga atgtcacaag gcttcacctc agcccaaatc ctctactaca
    24301 tcattctccc acaagccatc cgcaaaatgc taccagccat catttctcag tttgttaccg
    24361 tgattaagga taccagtctc ctctactctg ttatcgccct acaagaactc tttggagcca
    24421 gccaaattct catgggccgt tatttcgaac cagagcaggt cttcagtctt tacatcctga
    24481 ttgccctcat ctacttcagc tttaacctag caatttctag cctgtctcat atgctagcca
    24541 aacgttggca acaagccgca gaataatact cttcgaaaat ctcttcaaac cacgtcagct
    24601 tcaccttgcc gtatatatgt tactgacttc gtcagttcta tccacaacct caaaacggtg
    24661 ttttgagcag cctgcgggct agcttcctag tttgctcttt gattttcatt gagtataaac
    24721 agccactctc caattcaatt ggagagtttt ttgatgagag tagatattta tagacctcaa
    24781 tttgacacca tacattttct atgataaaat gtaacaaatt catttacaac aaatcgaaat
    24841 ctaaatgaaa tggaattttc tatgaaatca gtcaaaggac tactctttat catagctagt
    24901 tttatcttga ctcttttgac ttggatgaac acttctcccc aattcatgat tccaggacta
    24961 gctttaacaa gcctatctct gacttttatc ctagccactc gtctcccact actagaaagc
    25021 tggtttcacg gtttggagaa ggtctacacc gtccacaaat tcacagcctt tctctccatc
    25081 atcctactaa tctttcataa ctttagtatg ggcggtttgt ggggctctcg cttagctgct
    25141 cagtttggca atcttgccat ctatatcttt gccagcatca tccttgtcgc ctatttaggc
    25201 aaatacatcc aatacgaagc ttggcgatgg attcaccgct tggtttacct agcctatatt
    25261 ttaggactct ttcacatcta catgataatg ggcaatcgtc tccttacatt taatcttcta
    25321 agttttcttg ttggtagcta tgccctttta ggcttactag ctggttttta tatcattttt
    25381 ctatatcaaa agatttcctt cccctatcta gggaaaatta cccatctcaa acgcttaaat
    25441 cacgatacta gagaaattca aatccatctt agcagacctt tcaactatca atcaggacaa
    25501 tttgcctttc taaagatttt ccaagaaggc tttgaaagtg ctccgcatcc cttttctatc
    25561 tcaggaggtc atggtcaaac tctttacttt actgttaaaa attcaggcga ccataccaat
    25621 aatatctatg ataatcttca agccggcagc aaagtaaccc tagacagagc ttacggacac
    25681 atgatcatag aagaaggacg agaaaatcag gtttggattg ctggaggtat tgggatcacc
    25741 cccttcatct cttacatccg tgaacatcct attttagata aacaggttca cttctactat
    25801 agcttccgtg gagatgaaaa tgcagtctac ctagatttac tccgtaacta tgctcagaaa
    25861 aatcctaatt ttgaactcca tctaatcgac agtacgaaag acggctatct taattttgaa
    25921 caaaaagaag tgcccgaaca tgcaaccgtc tatatgtgtg gtcctatttc tatgatgaag
    25981 gcacttgcca aacagattaa gaaacaaaat ccaaaaacag agcttattta cgaaggattc
    26041 aagttcaaat aagtaatcat actctcctgg gatttcccag gagagttttt tatagccaaa
    26101 tgaaataaga ccggaacgat ggaattttgg cattcaaatc aattgataag aatattttat
    26161 agtggagtga aataggatac gaacaaattg attaggaaaa tcaaatgaat ttatagaaat
    26221 cttttagcag ttatgttgtc ctattctagt ttcaaaacgc tatagaagca gcattgtact
    26281 agtctagatt cagtttacta tactaaaacg agtagcttga aatcaaaaaa cccaccctca
    26341 caggcaggtt ttatctgtat tattcagcta gattatgctt taccttctga accgaatacg
    26401 tcgatacgtt cttcaaccga tgcttggata gcttttacac cgtcagccaa gaatttacgt
    26461 gggtcgaaga gttttttctt gtcgtattct gcttcgtttg cttcgtagtc acgagcaaat
    26521 ttacgagttg cgttagcgaa tgcgatttgg cattctgtgt taacgttaac tttggcaaca
    26581 ccaagtttga tagctgcttg gatttgctca tcaggaatac ctgatccacc gtgcaatacg
    26641 attgggaatc ctggaagagc ttctgtcaat ttttgcaagt ggtcaaggtc aagaccttcc
    26701 cagtttactg ggtaagggcc gtggatgtta ccgataccag ctgccaagaa gtcgatacca
    26761 gtttcaacca ttgctttagc gtcttcgatt ggagccaatt cacctttacc gatgattccg
    26821 tcttcttcac caccgatagt accaacttca gcttctactg agataccttt agcgtgtgct
    26881 ttttcaacaa cttctttagc caatttaagg ttttcttcaa ctggaaggtg tgaaccgtca
    26941 aacatgattg aagtataacc aacttcgata cactcaagtg catcttcgta gtgaccgtgg
    27001 tcaaggtgga tagctactgg tacagtgata cccattgatt caacaaggtt agcgatcaag
    27061 ttgcgagcaa ctttgtaacc acccatgtat ttagcagcac ccattgaagt ttggatcaaa
    27121 actggagctt ttttagcttc tgctgcgcgc aagatagctt gagtccactc aaggttgttt
    27181 gtgttaaatc caccaactgc ataaccgttg tcacgggctg cttggacaaa tttttctgct
    27241 gaaacgattg ccattttatc aggcctcctg tatattttta tgggtcatcc catttacatt
    27301 gttcatttta tcactttttg ccaaaaaaat ctagtttttc ccgcagtttc gattgatttt
    27361 cttctaactc catctatgta aaccctttct ctccctagtc ttggacgact tttggaaaat
    27421 ctataaagaa ggttaaacga ttctcctcca tctcgaaacg ataagctaat ttttcatgtt
    27481 ctaatagact cttaaccaca aagagcccca taccagaccc cttgaccttg cgactagcat
    27541 tgtcagaaaa agactgggct agtttttctt gttcctctga gctacagcta ttttcgataa
    27601 aaagttctcc ttctctttct ccaattcgaa ctaagccacc tggaacagag tgcttaatgg
    27661 cattgctgat gagattagaa agaatcaact tcataactga tgggtttaga taagcctgct
    27721 gatgggtcaa actattgtct atctggagct ctctttcctt ggctagcaag gcataatctt
    27781 tgaccagatt ttgcgtcatc tggaggaggt caattgtttc cctatcatct cgcaattcct
    27841 gcacagaaga gagggaaagt atctgcagaa catggtgatt gagttcatcc acaatcccca
    27901 aggcaactcc cagatactgg tctctatcct tataacgacc gatattctct ctcatatttt
    27961 cgattaggat tttcaaacta gccagcggtg ttttcaattc atgagaagct cctcgtagga
    28021 attcgacctt catcttctcc agctggagaa tggcttcatt cttttcatgc aagtccgcaa
    28081 taacagtcaa gagatgctgg tagaggctat tgatttgttc cttgagatta cctatctcat
    28141 ccttagaatc cacgcgcaat cgcacttggg aatccaggtc catcatccga cgggtcaccc
    28201 gcttgatttc caaaatcggt gcaacaatag tccgagcgta gatgtaggcc accaagaggg
    28261 aaatcagaaa ggaggccagc aaggtatagg gaagaaactg gagactgatt tgctccgctt
    28321 ccttttgtaa atccatggaa gctagaaact ggagaatcat agtaccaccg tcttgcgttt
    28381 tcacctcgcg ctcctcaata aagagagagg ttgtctggcg gtctgtgtcc agaggaagac
    28441 tgtccttgac ttctaacttg tcctcggtca tctcaccttt gacggtcccc ttgatatcac
    28501 tagtctggga atacaagtct aacacttgct cgatactctg cctatctttc ccttctaggg
    28561 actgggcaat ggctgttgcc ttttgaccaa tggtttcctg acgatgactc agataagtcg
    28621 aaggaaaaag aaaataaata gctaaatgaa ggcagataac cagaacacta aatatcgaga
    28681 aggtatagat aaatatcttt gcaaataaac ctgttcgttt cattttcgct ccaatttata
    28741 accaacattg cgcacagtga ggatacaatc caagtctagc tttttccgca attccttgat
    28801 ataaacatca ataacacggt caaagggaac ctcatctgtc gctttccaga cggcatcgat
    28861 aatctgagat cgagtcaagg cccggccttc atttttcact agatagtcca gaatttccaa
    28921 ctctttggca ttgataggca cttcttgacc tgcgatgctt gcactgtagc tttcaaagtc
    28981 caccttggta tccttgtaag aaaagattcg tcctgtatcg tagtagcgct tgaaaatcgc
    29041 gtccaccctc acttttaaaa gggagaggga gaaaggtttt tccagatagc catctgccaa
    29101 agaggcaaag gcactcatct tgtattcctc atcctgaaaa gctgtcaaca tcaagacagg
    29161 aacctgactg gttttacgaa tctcagctag gacttctaag ccgttgagct tgggcatctg
    29221 gatatccagt aaaaccaggg ccacctcata gctagaaaat tgctccagag cttcctgacc
    29281 gtccgctgcc tcaatagttt catagccaca atccgtcaaa taatcactga ccccctcacg
    29341 gatcatctct tcatcttcta caattaaaat tttcatactt taactgctct ctatttttta
    29401 tttttcttag aataaatacc tactctattt tctattatag tctcttgctg gccttttgtc
    29461 tgtaagtaac tgaccactag ataaaacgtt gtgaaattcc tttctcataa attccataac
    29521 tttagtatat tatatttaag cactaaagta caaagaaagc aactgaaagc aatgattttc
    29581 accactgctt tcagatttat tttgaattgt taaatagcca ttcctatcca ctattcttga
    29641 atagaaacac aagatgcaat ctttattcca gactcatttt ttcaaattta ttcaccatcc
    29701 agcaagagct cttttggttg ttttctaagg agattgcttg aagcaagcgc cataacgaga
    29761 accactagaa ccaaggcaag gacaaaaatg atgataaagt ctgatgtctg aatagaaatg
    29821 tctaggctcg acaaggtctt gctaaagcca tctacttctg caccaccacc aaggttagag
    29881 gcttgagccg ccttactagc ctgtttggca acacctgaag tcacattggc aaggacagtg
    29941 tttccaattg cacgggcagt gtaattagct aggaagtaag cagaaactag agcagggata
    30001 gcaatcaaga tagattcggt gatgaattga cccaagatac ttgcctgctt gaggccgata
    30061 gagaggagaa ttcccacttc cttgcgacgg gcgttgatcc aaaggctgag caagagggca
    30121 aggaggagaa ctgagaagct caagctaccc cagaagagga ggttggccat cttgtacata
    30181 ccagagatag attgctcaag agctgggtag ttagaggagc tcttgacgag tgtgtagctc
    30241 ttccagttga taccactgat gccattcaac tctttcataa catcatccaa gttcttgtct
    30301 gctgttacaa agaaggttgc gtccccataa atggctgtgt cttctgtgta tccataaagt
    30361 tttgcagcag tgtgaatgtc tgtaatagct gtgttttcgt aaagttcttg tgagtaggtt
    30421 actgctgact tattatgacc atcaaagagt cccttgattg tcacttcaac tgtttccttg
    30481 gctccttttt cattatctgc atcgtagata ttagagtcca gtttaacctt gtcccctact
    30541 ttccagccgt gtttggctgc caagtccttg tgcaagagga ttttatcctt gtcgtcgttg
    30601 gttaagtgct ctccttcgac tagtttataa gaaccagaga caaacttgtc ttctttagag
    30661 gagtcattga cacctgtaat catcaagcta cttccaaaac gtttggcacg atcagcagtg
    30721 agattcttct tggtttctgg cgtttcaatc aggtcatatc cagtcaaatc tccgatagcg
    30781 ttgatacgtt tgacataaga ctcaatggcc ttgttttcgg tgattttttt gatgtcttca
    30841 cccttgatat tcccagcacc gcgaggcgtt ccttggttga cgcgacgatt gatttgcatg
    30901 gagaagctat tggtgatatt tttaaaggtc tcctgagaag ccttggcagt agctcccttg
    30961 attgacaagc cgaccaaact caagctcgcc atgaggagaa taatcaggaa gatgacaatc
    31021 gatttgaaaa acttccttgt aacataggca aatgcgttgt gtaacataga ttccctttct
    31081 agattttgtt ttaatcattc tattaaagta agctcaaatt atttactagt attgcgcgtt
    31141 tcagtcagtt tcttatcctt taattcaagt gtaatatctg acgcttgtgc cacttcttta
    31201 ctgtgagtta cgacaatcac acatttacct gttttctggg caagtgattt gagtagttcg
    31261 acaatatctc cagcagtttt aggatccaga tttcctgttg gctcatcagc tagaataact
    31321 ggagcttctg agaccaaact gcgagcaatg gcaacacgtt gctgttgacc acctgataac
    31381 tggagaacat tccgcttgat ctggctttca tccaaaccaa gctcaagaag tgtattcttg
    31441 cttgcctttt tgttgaccaa tcggatattt tccagcggag aaagataatc tatcaagtta
    31501 taattttgaa agaccaggga aatatggtgc atgcgatggt aagaatagcc cttcttacga
    31561 atatcctctc cttgaaaaag gatagaacct tcaacaggac tatctagacc agcaagtagg
    31621 gacaagagtg tggattttcc tgctcctgac tccccaataa tactgtaaaa ttttccgggt
    31681 tcaaaattat aattgatctg atataggact gcttcagcag tattcttata acggtaggta
    31741 acatcttgta attgtaataa agtcatgatt tctccttctt aactaataga tgataaaatt
    31801 tctttcggtg attttctaaa taagaatagg aaacaaaggg ctacagataa gcaactaagc
    31861 agaactagaa aaacatagga ttctgcaaaa gataagatgc tagttgataa actgcttgct
    31921 ttggctagtg tatcttgtaa gcttgcctga tctccacttg ctagtagagt ttggagtagg
    31981 taagttgtga ttccgtttcc tgcaacaaat gctggaagca aagctccaag agataccaaa
    32041 actacctcta aacagaattg taggaaaatc gagctcttgc cttttccaag tgcaagtaaa
    32101 atccccactt catagacccg ttctctcaac cagagagaca aaaccagaat taaggctcca
    32161 gctcctgcta tcaacatccc ataaaggaag atggtcagga aggtttggaa agttgcaact
    32221 gagtctttga tttgttcaaa agccttgttt tccttttcga cttggtagcc ttgattttcc
    32281 aaggccaagt tttctacctg cttcatgagt ccgtccattt ccttaggatt ttctacatag
    32341 aagcgtgctg cactgacttg agcttcacta ttgcccaaaa gggtttggct actttcatag
    32401 tctgtaaaga cttgattttc actgaagtca gaagacaagc ctgtgaattt ctcttgtttt
    32461 ttaccagaaa agatgccgat aatctcaaac tctactgttt gtccttttcc agattcagac
    32521 tgaccagcat ccaagccaat cttgtcatga agtgaaagac cgttcttctt agccaattct
    32581 tcgtggataa ggattttctt ggaattccct ttttgaaggt gtcgcccttc ttttagattg
    32641 aaagccgaac tggtaaaggt tacatccttg gatgaatcct caagagccgt taagctaacc
    32701 aagttattgt ctgcagctga taaatcatca cgctccacgc tctgctcgcc agtcactgct
    32761 tccttgtctt ttagttttgc gaccgtctca agttcaggag agacattttc cagcccctta
    32821 atcttgctta cagatgctag gtctgacaac ttgaatgtct gaccattctc tatcttctta
    32881 atagaaaaag atgtattgag tgatttataa agattgcttt ctactgtttt gttggacttc
    32941 atcagagtca aacaggctga aattccggcc aataagacca ataaaatcag aaataaaata
    33001 aaacttctca gtcgctttct gctgacataa gcccaagatc tttggattgg attcatttgt
    33061 cacctccata tttgtaagac tattataaaa cccaaatatg aaatgtttat gaaatacgaa
    33121 aaaaaatatc gagtagggga taatctctag cccctctcac accaccgtac gtgccgttcg
    33181 gcatacggcg gttcaactaa cttttaacgc atgtcgttca aggtaataat ccaaacacga
    33241 aaccagtcca cgtttttcaa gggctggttt tgatatagca cgtttaagta ccgacttctg
    33301 agctactaat tgatagtggt cgccccagcc agatacctta tctgctatcc atttaggaac
    33361 ccctaactta agcaatcccc ataatcgtct cgatttcttc ttccattgct tccagataat
    33421 cactcgtagg cgagtacgca agcgctcatc tatgctagtg actatacttt tcatatttat
    33481 aattcattcc tttcgtttca ctcaaggcac aacacagaat gaaaaagtgt tgtgatcttt
    33541 attttgtttt ataataatag tgagaaagcc tatcactact acaaatcacg gggaggtgaa
    33601 taagtgagtg gtacagccac tacctcgcat attttgtcac atcatttaac ggtacataat
    33661 aagttgtacc atctgaataa gttgctacaa tatcatttgc atgctctcct tcacctttag
    33721 caaaggttgg agctcctgct ggatgatttt tatttgcctc tttcaatttt tcaataatgg
    33781 catttttttc tgtatctttt atattatcag gatttttcac taagattttg tctggatatg
    33841 tcggtttagc agaaacaatt tttactgtta tttctttttt attcgaagca cttgtccagt
    33901 ttccagcatt atctttagta tttaatttta cagtaattcc tgaactagga acttcagtag
    33961 caggttgatt atcaacatta ttcaacttta atttcaaaag agctgttgca tcagacgttt
    34021 tatcaatcgt tatatataat gataaattgt tattataacc agtttcttca tatttagctg
    34081 tttgtgagct acttgaaaca gaactgaaat tatacccact acctccctga ttatcttcaa
    34141 tgcttacatc taaatgaact tccccactat tatttggatt agcaacaact gttatagtaa
    34201 aataacataa aattcacata aatagattag ggaaatcaaa gcaacttcta ggaatgtttt
    34261 atactcttcg aaaatctctt caaaccacgt cagcttcacc ttgacgtact caagtacagc
    34321 ctgcggctag cttcctagtt tgtactttga ttttcattga gtattagcag tcacagtgta
    34381 ctttcccagc atcaagccac tataactctg cacataaaaa tggagaagat ggcaatcctc
    34441 ttctccaaat attaacttct ttacaaacca actatagttg acaaagaacc taaaatcaat
    34501 tgataacaca aggtcaggtc ggtcaactct ttcaactgaa gccctgtcaa ctcttcccat
    34561 ttatcaatct tgtattggag agaattgcgg tgcagataga gttgctgggc tgttttagtg
    34621 agaacagcac tattttccca aagagagaga atgatttcct gaatctgatc ttgatccaaa
    34681 atcatctggt gtagacattc tttaattggc ttcaagtcca cgagtctttc tcccatactc
    34741 caaagataga gctgagaaaa agtatgaaca ccttggtgac cctgacgcca ccatgtcttg
    34801 aacaaatccc gctcagcttt gattaagtct gatagggctt gatgtcctgt ctgagaccaa
    34861 acctgaccca acatgataga aagacgaagt ccaaagtcat actcaactgc ttcaatcata
    34921 tcacttaaaa tatctcttac agaagtgtat ttgtcttgtt gaagcacgaa aacataatcc
    34981 tgagctccga cctgtaacac tgtctgacaa ttcggaaaaa gagtccgcat catatctagc
    35041 caagaagcca gattttcctg ctgaaaataa gaaagatggc aataaaccaa ctgaatcttt
    35101 ttaaaaactt gcggtgcctg tcccttgccc tcaaccagat aggaatacca agggtttagc
    35161 gaacgagcct gctcctgctg ggtcaaaagg acaaccaact gcttttcacg ctcgctgagc
    35221 ccagcttcct ccagcaaaat ccactgctga gaagctaaag ggagcgtgag atagccctct
    35281 ttctctactg gttggtctga aatccgagcc tcaggaaacc agtcttgtag ttcttttgcc
    35341 ctcatgttct agccctccac tttttggatg caccatgaaa ccaaactctc aagacgttcc
    35401 agattctcag tcatatggag atagcccata accgcttcaa atcccgtgga catacgataa
    35461 gtcacgacat ctgcattttt agcctttgtg tggctattgg tattgcggcc acgtttgtag
    35521 atttcttctt ctttttccgt taggacctgc tcctccaaca tgagagcaat caggcgagcc
    35581 tgagccttgg ctgacacata cttggttgct tcttgatgga gtttattggg tttggtcata
    35641 cctttgagga tgaggtgacg gcgaatatac atagaataca ccgcatcccc ctcaaaggct
    35701 agcgcaatcc cgttaatgag attgacatca atcacgtgtc cacctcactc catccttggt
    35761 atcaaggagc ttaattcctt gagtaaccaa ttggtcacgg atttggtctg ctgtcgcaaa
    35821 gtcacgattg gcacgcgcct cttggcgttt ttgaatcaag tcttcaatct ctgcatccaa
    35881 aacttcctca acaaagacaa ttccaaaaat ttctaacata tctgcaagag cttgcttgac
    35941 acttgcatca tagttccctg aattgatcca tttggccatt tcaaagacaa ctgtgatacc
    36001 gttggcagca ttaaaatctt catccatagc tgctacaaac ttatctttaa agttttgtaa
    36061 ctcttgggca tccacgtttc ctgtaaatgg ttgttcgtaa gtattcttca gatacttgag
    36121 attggtctcg gcatcgcgaa ctgccttttc cgtgaagttg ataggcttac ggtagtgctg
    36181 ggtcgcaaag aagaaacgaa gtacttgccc atcaagagtt ttaagggcat cgtgtaccgt
    36241 aatgaagtta cccaaggact tagacatttt gacattgtcg atattgacaa agccattgtg
    36301 catccagtag ttagcaaaag ccttgcctgt tttagcttca gattgggcaa tttcattggt
    36361 gtggtgtgga aactctaggt cagctccacc accgtggata tcaatggtat cacctaaaat
    36421 ctctgtcgac atgactgaac actcaatatg ccaacccgga cgtccaggtc cccaaggact
    36481 atcccaagaa atctcacctg gtttggaaga tttccataga gcaaaatcta caggattttc
    36541 cttacgagcc gtttcttcat cggtacgacc tgaagcacct agatccaaat cttccaaggt
    36601 tttattagcc aatttagcat agttgtggga tttttctaca cggaaataga catccccttg
    36661 actctcatag gcaaagcctt tctcgatcaa gtcttccaca aaacggatga tgtctgccat
    36721 aaactccact acacgcggat ggcgagtcgc aggtttcacg cctaaggccg tcacatcctc
    36781 acgaaaggca gcgatgtact tatccgcaac ctcctgaggc gtgatacctt cttccctggc
    36841 acggttgata atcttatcat ccacatctgt aaaattggaa atataggcaa ccttataccc
    36901 acggtactca aaatagcgac gaatcgtatc aaaagctacc gtcgaacggg cgtttcccac
    36961 gtggatatag ttgtacaccg ttggcccaca aacatacatc ttgatcttgc cgtcctcaat
    37021 cgggacaaat tctcgcaaat cacgagacat ggtgtcatag attttaatca taaatcataa
    37081 tcaagaaagc tgaaatccaa gaacaattag tttcatcact aaaagttcaa gtaaatttca
    37141 gtccgaatat ctctacactt cggaatccct tgctcctttc tcattcagat aaaccacctg
    37201 agtctgtttg acaaagccaa ttttttcata caaacgtttg gcacctacat tgctatcttc
    37261 cactgcaatc tgaaattcct tgtcattttg ctcaattagt tggttgacga gggattttgc
    37321 taagtagctt ccatagcctt tcccacgttc aggttccgat attgctaaac cgtagaagta
    37381 attcgtatta gtcgataaat ctaccgtgca agtcccaata acctgaccgt cttttaacaa
    37441 aatatataag cgactttctg gatccttcag agcttcagcg acatatctat ccacaacttc
    37501 tctcgattca tgttcctctg aaaatgcctg aaattttaat tgactaattt gatcctgata
    37561 cgaactatct gctaacaaaa cttcaagatg ggaaacattt gctaacggat aaggtcttct
    37621 atccttacct aaccaagttt ctgtctcttc atcctcgatt agtccccagt tactggcaaa
    37681 gtcaggatga ttctctaaaa aaatacgttc tgtctgaaaa gtgactgacc gaatggggaa
    37741 agaagctgtt tctctctcaa aactagtaaa caatgcacgc gcaatcccct gacggcgatg
    37801 acctggatga accagtatcg ttacttctac atcttggtca tctgcataaa cagttaataa
    37861 accaacaagt tcgccttttt cataataaag gaaaaaggcg ggcatgtttg ggtcaaaatt
    37921 aagcatgtta gagagatagg gatcgcgata ggtaccgtca tagttttggc aacagttaat
    37981 tacttttttc gcctcagata gctcctcttg gcttaacttg tttcttgctt gaatcatata
    38041 ggtatcctct acaaaccaga cgatctgtga ctggcatctt tagcctgctc gagtttattg
    38101 acataatact ctcgtttttc ttcgacttcg tgaatgacag gctcatcttt cttaccatga
    38161 agacggacaa tcttggccgg aataccgaca accgtcacgt cactaggtac atctgctacg
    38221 acaactgctg cagcaccgac cttggcattt tcaccaattt ccacaggccc gataacttgg
    38281 gcatgggctg atatgagggc tccctttcgt acagtcggat ggcgtttgcc acagtctttc
    38341 cctgttcccc cgagagtcac tccgtgatag agaagaacgc ctttttcaac aatcgctgtc
    38401 tctccaatca ccagaccaga accatggtca ataaaaacac ctgaatcaat ctgggctcct
    38461 ggatgaatct caatctgagt ccaaaagcgc caaaactgac tgtacatacg agctaatagt
    38521 ttgaagccgt gcttccagag aaaatgcgag agacggtggg ccgccaaggc cttgacacct
    38581 ggataagtca gcaaaacctc caaagtggtg cgggccgctg gatcattttc ttttacaata
    38641 tcaatggttt cgcgccacca ccccatacat ttctcctttt cttattctga atcttttggt
    38701 gtttctgtaa attctttctt aggtttgtga tccttttgat gacgtgggcg gtgagggcgc
    38761 tcagactttt cacctttttc atcatgctca ggttttggcg gacgaggtag aagagccttc
    38821 atagaggcat cgatacggcc tttttcatca attttgataa ccttaacatc aacttcatcc
    38881 ccgatttcta ccaaatcctc tacacgattg gtacgagtcc aagccatctc agagatatga
    38941 acaagggcat ctgtcttatc aaagaggtta acaaaggcac caaatttctc gatacgaacg
    39001 actttagcac ggtaaacttc atccactttg gcttcacgaa ccaaaccagc aataatttct
    39061 ttggcacggt taatagcatc ttggtcacta gagtagatag acacatttcc ttcttcgtct
    39121 atatcaatct taacacctgt ttcagcgata atcttgtcga tggtttctcc acccttaccg
    39181 atgacaatct taatcttgtc cacatcaatc ttgatcgtat caattttcgg agcagttgga
    39241 gccaattctg gacgaacttc tggaatggtt gcttcaatga catcaaggat ttcaaaacgc
    39301 gctttcttgg cttgagcaag agcctccgtc aagatttctg cagtaatccc ttgaatcttg
    39361 atatccattt gaagggctgt aatcccatca cgagtacctg caaccttgaa gtccatatct
    39421 ccaaagtgat cttccaaacc ttggatatct gtcaatactg tgtagttatt tccatctgag
    39481 ataagtccca tagcaatacc agctactggc gccttgattg gcacaccacc agccataagg
    39541 gcaagagttc ccgcacagat agaagcttga gatgaagaac cgtttgattc caaaacttct
    39601 gctactagac ggatagcgta tgggaattct tccaagcttg gcaagacttg agcaagagca
    39661 cgctcaccaa gggcaccgtg accgatttca cgacgacctg gcgcaccgta acgacctgtt
    39721 tcccctacag aatattgagg gaagttatag tggtgcataa agcgtttctt gtactccaga
    39781 tccaaaccat caatgatttg agtttctccc atcggagcca aggtcaagac agaaagggct
    39841 tgcgtttgtc cacgagtgaa gagacctgaa ccgtgcacac gaggaaggaa gtcgacaacc
    39901 gcatccaaag gacggatttc atcgacctta cgaccatcag gacgcacctt gtcttctgta
    39961 attaaacgtc gcacttctgc gtgttccatt tgttccaaga tttcagccac atcacgcata
    40021 atacggtcaa attcttcgtg gtccgcatat ttttcttcgt aaacggcagt cacttggtct
    40081 ttcactactt gagtcgcagc ttcacgggcc aatttctctt ctacttgaac tgccttttgg
    40141 aggtcactgt tgtaggctgc aatgatttca gcttgcaatt cagcatccac gtgaagcaat
    40201 tccacttctg ctttttcttt accgacagca gcaacgattt cttcttggaa ggcaatcaat
    40261 tctttgacag cttcgtgccc tttaaggagc gcttccaaca tgatttcttc tgacaattct
    40321 ttagcaccag actctaccat gttgatagcg tgcttggttc cagctactgt caattcaaga
    40381 agagattgct ctgcttgttc ttgacttggg ttgatgatga tttggccatc tacatatccc
    40441 acttgtaccc cagcaattgg tccgtcaaat ggaatatctg aaatagacag tgccaaagat
    40501 gaaccaaaca tagcagccat tggtgcagac gcattttcat cataagaaag cactgtattg
    40561 atgacttgga cttcattacg gaaaccttcc gcaaacatag gacgaatcgg acggtcaatc
    40621 aaacgcgctg tcaaggtcgc atctgttgaa ggacgtcctt cacgtttcat aaagccacca
    40681 ggaaacttcc cagccgcata cattttttct tcgtagttga cttggagtgg gaagaaatcc
    40741 ccagttgcca ttttcttaga cataacggca gcagtcaaga cagttgactc accgtaacgt
    40801 acgacaacag atccatttgc ttgcttagca acctgaccag tctctacaat taactcacga
    40861 cccgcaacag tcgtttgaaa cacttgtttt gccattttaa tcccctttgg attgatgaaa
    40921 ttatacgcct tgcctacaaa gatcaagata ccaaggacgt caaaaacaaa gtaaaaatag
    40981 gaaactgacg aagtcttcga tgaagacaag acagtttatc ttttttacac agcttttcgg
    41041 ccgtgttcaa ttacacaaga tattttggac ggttcggctt gccgaacatt tctgtagaaa
    41101 aataggaagg tgacgtcgca ctcgacgagt gctaggaagc ttatcttttt tcctaagaaa
    41161 tgagaccaaa attcaattac tcaagatact aagccgtcaa gcaactcaaa ggaaaatagg
    41221 aaatcgaacg acggagcgac tgctcctagg aagatttatc tttttccaca gagttgtagg
    41281 cgagttcagt tttcaagata taaatcatta gaaaggttta atactaaagt atctaaagct
    41341 ttcacgctaa tcgctatcgg gcgattagct aaatgcttta ctaactctct cgtcaaataa
    41401 catcgatttg actcactcgt gtcgttaaat cttacagttt aaatgcattg tattatttaa
    41461 taccttcatc tttgtatcaa gtacgtacag aatctatttt atcatatttt tcttaaaaag
    41521 tgaggtcttt accattaaaa aggaaccatt cccctcacct gagaagaatg gtttgctttt
    41581 attatcctag agactggtga ttaaacaagg catgggttgc ttgatggatg tattttgctg
    41641 tatcagcatt attcatcgta tagagatgca caccggcaac atcctgagtt accaagtcca
    41701 cgatttggtc cactgcatag gcaagccctg ctgctctgag cgactcaggg tcatgctcat
    41761 acttgtctaa gatggcttta aatttgcgtg gaagatggat attctcacaa gtcttcaaga
    41821 gtcggagagc ctgatttcga ttcagaattg gcataattcc tgcatgaatg ggaacatcaa
    41881 tcccagccaa gatgcacttg tcctgaaaat catagaagcg ctcattgtca aagaagagct
    41941 gagttacgag gctcgaacag cctgcatcca ctttcttctt aagattttga atatctgaaa
    42001 tctgatttgg cgaatctgga tgcccttctg gatagcaagc tccaataata tcaaagtgag
    42061 gggtttgttc cttgataaac tcaatcaagt cggttgcata gcggaaatcc ttttgtggtt
    42121 ccacgtctgg aataatatcc ccacgaagag ccaagatttt ctgcacccca actttgtcca
    42181 agtcagcaat agtttcagca accttgtcct tagttagata aatagctggc aagtgggcaa
    42241 tggtcggaat cgccaaatca ttttggataa agtcagccaa acgaaccgtc gtttccttga
    42301 tattaaattt attattgctg gcagttacac tgataaaatg gggagccaac tcctgcatat
    42361 cctgcaaggc tgaaataatg ttatcattac ccacggctgg gtttggaggg aacacttcaa
    42421 atgagagtga cggtgtttgg cgtgacatat gtaataacct tttctagttg atttcttttt
    42481 gaacaaccac tgtatggaga gaaatccaat cttacaattt ctcacgcgca gctttagctg
    42541 cttcaacaag gcggatcaag ctttcttttg tttctgggat accacgtgtt ttcaaaccac
    42601 agtcagggtt gatccaaact ttcttgcttg gcactttagc aaggatggct tcgattgtgt
    42661 tgtcgatttc gccttcattt ggtacacgag gtgagtggat atcgtaaacc ccaggtccca
    42721 cttctgtttg gaagtttttc gctttgagtt cgtccaagat ttcaaggttt gaacggctag
    42781 cttcaaagga aataacgtct gcatccatgt tatcgatagc tgggatgata tctgtaaatt
    42841 ctgagtaaca catgtgagtg tggatttgtg tgtctggcgc tactgttgag tgtaccaagc
    42901 agaaggcagg aatagcccag tcaaggtagt cttcgtacca gtcgctacgg cggagtggca
    42961 atttttcacg aagagcagcc tcgtcgattt ggatgatttt cacaccagca gcttcaaggt
    43021 caagtacttc atccttgata gcaagggcga tttggagagt tgaatccttg atagagatgt
    43081 cttcacgtgg gaatgaccag ttaaggatgg taacaggtcc agtcaacata cctttaacag
    43141 gtttgtttgt acgactttgt gcatagctag accatttaac agtgataggg ttaagacgag
    43201 tgacatcacc ccagatgatt ggtggtttta ccccacgcat accgtatgat tgtacccatc
    43261 catttttaga gaagaggtat cctgacaagt tttgaccgaa gtactcaacc atgtcattac
    43321 gctcaaattc accgtgaaca aggacatcaa agtcaatatc ttcttgccac ttgatccatt
    43381 cgtcaatcgt ttcagcaagg aaagcatcgt actctttttg agacaattca cctttacggt
    43441 aagccaaacg tttggcacga acttcttttg tttgagggaa tgaaccaatc gttgttgttg
    43501 gaagagctgg aagtttgaaa gcttcttctt ggatagcttc acgttctgca aaggctggca
    43561 aacgagtgta gtctgcgtct gtcaagccag cgatacgcgc acgaagttca gcattttcac
    43621 caacacgctc agtcgcaaaa agttctttgt tggctgcaag agcttctgaa ccttgaccat
    43681 tgcggatagc atccaagtca cggatttcat ccaatttttc aactgcaaag gcaaagtggt
    43741 tcaagagtgc tggttcaaat tcttcattag cagttgtaaa tggcacatga agaagtgagc
    43801 aagagcttgt caaaacgatg ttttcagctg gaatttgctc aagaacagcc aagctctttt
    43861 cgtagttgtt gcgccagatg tttttaccat tgacaatacc tacatagaga gtcttgtcag
    43921 ctgggaagcc acctttaacg agttcaagag ttttcttacc ttcaacaaag tcaagaccga
    43981 tagcatctac tggtaagttt acaaggtcag cgtatacgtc acgaacatca ccgaaataag
    44041 tttgaagcaa gacttcaaga ccttttttgt cagccaagag tttgttgtaa aggttcaaga
    44101 agagagcttt ttcttcagct gtcaagtctt ttacaagagc cgcttcatcc aattggatgc
    44161 gagtcgcacc aagttcagcc aatttagcaa aaacttcttg gtaagcagcc actaagctat
    44221 ctacgaagtc gtctgctttc acgccttctt caaagtctga caattgaagg aaagtgaagg
    44281 gacctacaag aacaggacga gtgttcaatc caagttcttt ggcttcttgg aactcatcga
    44341 aaatcttgtg accagccaat tttacttgag tgtctttttc aaatttagga acgatgtagt
    44401 ggtagttagt gttgaaccat ttcttcattg gaagggcgcg aacgtcccct ttttctccct
    44461 ggtaaccacg tcccaaagcg aagtagcgct caaggtcaga caagtccaag ttttgaacgg
    44521 atgcaggcac cacgttgaaa aggaaagccg catctaggaa gttatcatag tgagaaaagt
    44581 catttgatgg aatttcagtg atgccttttt ctttgacaat gttccagtgt ttagcacgca
    44641 agtcttttgc tgctgctaaa agttcttctt ctgagatttc ttttctaaag tatttttcag
    44701 ttgtaaattt taattcacgg aattcgccca aacgagggaa accgatgatt gtagttgaca
    44761 tgatgtgtcc tccaaaattt gttgttgaaa ctatcttaac agaaaagaaa gcgtctgtat
    44821 aattgtaaaa aattagggtt tgatatagtt tgaaactata tatctgtttc ggacaaaaga
    44881 aaaagacttg aagcaaacgt ctcaaatcct ttgtaattct tactttacag ctatattcca
    44941 attagaatac taaaacatgt tattagtaat tcttataagt gactatgacc tgttattaga
    45001 aaagactata actgattcta gtcaactttt tccctgttca agtgggacga ttgctagtgt
    45061 ctttcctaaa ctggctagga cttttaagac tgtatccaac tgaggactag tctttcctgt
    45121 ctccatccta gctatgacag gctggcttat tccactgact tcttccagct ttttctgact
    45181 gattccttgt tcatacctag cctcaatcaa ctcgctcatg atagccactc gcatatcact
    45241 ttcaaggatt tcctccttgc taaagagctc agatggacat ccttccaatt actcccaata
    45301 gcactattct tcatcactta accctctttt ttttacgtct atgtattttt aaaaaaatga
    45361 gcgaattatg attcgataga ttgaccagtg ggtttaaagt tggtgctagc ctatttctta
    45421 agcgattttc cttttctagg ataaagtaga tcctgcttgc ttaaccccaa ttttccacga
    45481 tgaatccaat agtaaatggt tgaaattccc acgttaaccc ctttagccat caccatcatt
    45541 tcaggcgaaa atttttggtt atgtatagtg gagaatcttt tcctttagtt tcttaagact
    45601 gttgagcgta gtcggcagaa taaatctctt tgaagcgccc ttttccaaga cattgtcgga
    45661 ctgtcccacg cttgatttca gtgtggatag tttgaggagc ttttccaagt agagaggcaa
    45721 tttctctatt tgattttcct tcttttttcc atcgttcgat taagcgacgg ctatcgattg
    45781 tcaaatgttt gccttttgta gtataattgt cttgcatttc tgtgcctttt aatcatttca
    45841 atcttaaatt ggactttttt tacttgggtt gtacttaatc tatgaggaag acaagaaaaa
    45901 gaatatcaat caagtaaagt cacaaagtta cattagctcc gagcaaccat tgcaaattga
    45961 ggtactcaca caatgattaa aacatttctc tctgcccttt cggtcattct cttttctatc
    46021 cctatcataa cttattcttt tttcccatct tctaatctta acatttggct atctacccaa
    46081 cctatcttgg cacagattta tgccttcccc ttagctactg caactatggc tgctatttta
    46141 agtttcttat tttttttcct atctttttac aagaaaaata aacaaatacg gttttactct
    46201 ggcattttgc tcttactatc gctcatatta ctattattcg gaacagataa aaccctttct
    46261 tctgcatcaa ataagactaa aaccttaaaa ttagtaactt ggaacgtcgc taatcaaata
    46321 gaagcacaac atattgagcg aatttttagc cattttgacg ccgatatggc tatattccct
    46381 gaactagcta ccaatatcag aggtgagcaa gaaaaccaga gaatcaaact attgtttcat
    46441 caagttggac tttctatggc caactatgat attttcactt ctccacctac caatagtgga
    46501 atagctcctg tgactgtgat tgtcaagaaa agttatggtt tctatacaga agctaaaact
    46561 tttcatacaa cacggttcgg gacaattgta ttacattcga gaaaacaaaa tataccagat
    46621 atcattgcct tgcatactgc tcctcctctg ccaggtttaa tggaaatctg gaagcaagac
    46681 ttaaacatca ttcataatca attggcttca aaatatccaa aggctattat tgcaggtgat
    46741 tttaatgcaa ctatgcgtca tggagcactt gcaaaaataa gctctcatag ggacgcattg
    46801 aatgcacttc caccttttga aagaggaact tggaatagcc aaagtccaaa actttttaat
    46861 gcaacgatag atcatatctt attgcctaaa aaccactact atgttagaga tttagacatt
    46921 gtaagttttc aaaactctga tcatagatgt atttttacag aaatcacatt ttaattattt
    46981 tatataaaat tccccctcta gtgttcataa actagagggg gaatttgtat cctactatcg
    47041 tttaacgcac ttctgcattc actttttctt cgagcgatgc ttggattttt tccatatagc
    47101 gtgcgacttc ttcgtccgtt aagctatctt ctggattttg gaaggtcaag ctataagcca
    47161 tggacttcat accaagtccc aatttctcac ctgagaagac gtcaaagagt ttgatatctg
    47221 tcaaacgttt cacgccggca gcttggatag catctacaac ttcttgatga gtcacttctg
    47281 ccttgaggag aagggcaacg tcacggctga ctgctgggaa tttcgtgatt tccacaaatg
    47341 gaacagcagg ttggagcgcc ccttcgatgg ctgaaaggtt aagctcagct acatacgttt
    47401 ctggaatatc gtaagccttg gcagtgactg gatgcacttg gccaaggaaa ccaagaactt
    47461 ggtcaccgag tgaaatcacg gctgtacgac ctggatgaag gctagcgatt tcagatgttg
    47521 ctgtataggt tacttggagt cccaaacgag taaatagggc ttcaaggatt cccttagcat
    47581 agaagaaatc aactggaact gctgctgttt ggaaatcttt ttcagcaacc aagcctgtca
    47641 aggcaaaggc aaagctgttg atctcatttg gaagttcttc ttttggatta cctgtttgtt
    47701 caaagacttt tccaatctca taaagggcca agtttttatt cttacgagcc acgttgtagg
    47761 caacggtatc aaggatccct gaaatcatat tttgacggag gactgaacga tccacagtca
    47821 ttggccacat gagttcagta aggttacttg gttgagctgt gaactcaact gctttttcag
    47881 gagttgtcag agcataggtg ataatttctg tcaaacctgc tccttcagca atggtacgaa
    47941 cttgacggcg gagtttttgt gtcacagtca attcaccagc tgtaccatcg tcttttggaa
    48001 ggctggttgg caagcggtca tatccataga tacgagcgat ttcttcaaag agatcagctt
    48061 cgattgtgat atcccaacga cgacgtggta cgctgactgt aaagctgtct gcatttccag
    48121 aaagaccaaa gccaagacga cggaagacgt cttcgacatc agcataagac agctcagttc
    48181 cgaggacacg gttaacatca gcaagagttg aagaaacttc tacatctgaa gtatcaagct
    48241 cacccgctga aacgataccc ttacgcaccg tcgcacctgc aagttccgca atcaggctag
    48301 ctgccgcatc aagggcttca ttaactgttg ccacattaat tcctttttca aagcgagaag
    48361 atgactcaga acgaaggttc aggtgaccac ttgtcttacg gatagatttg ccattgaaaa
    48421 cagcagcttc aaggacaaca cgactagatt tttcagagat ttctgttgct tgaccaccca
    48481 tgacacctgc aagggctact ggcttgtctg caacagtgat gactaggtca ttcacgtcca
    48541 agtcacgttc ttcaccgtcc aaggtcacca atttttcacc agcacgcgct tcacgcacac
    48601 ggatgtcagt cccttcaaat gtgtccaagt caaaagcatg cataggttga ccaaagtaga
    48661 gcaggatgta gtttgtcacg tctacaacgt tattgatggg acggatgcct tcgttcatga
    48721 gaaggttttg caaccattgt ggacttggtg cgatagtcac attgtccaag atacgagctg
    48781 catagtaagg cgccttgtct gtcccaatgc tgacagaaag ggcatctgcc gcagcttcat
    48841 tagtttctgt tagagtaaat tctttaaagt tgactgcctt gtcatagatg gctgccactt
    48901 cgtgagccac tccacacata gaaagggcat ctgcacggtt tggtgtgatg gaaagttcga
    48961 tgatttcatc atccaagtct aggtaagaaa agacttcctc acctggcacg gcattttctg
    49021 gcaagatttg gatgccatct gcgaattcct taggcacaac tgagtcagaa attcccaatt
    49081 caccaagtga acagatcatt ccaagtgact ccaaaccacg gatttttcct tttttgattt
    49141 tgtagttatc agcgatacga gctcctggaa gagccaccat gaccttgatc ccagcacgca
    49201 catttggggc accacaaacg atctgacgct cttcttcttc gccaacgtta acctgacaaa
    49261 catggaggtg agtctctggc acatcttcgc aagacaagac ctcaccgacg acaatttttg
    49321 agagaccagc agctggtgat tcgacaccct ctacctcgat tcctgtagtt gacatttttt
    49381 cagccaactc ttgtgatggc acatcaatgt ccaccaattc ttttaaccat ttataagata
    49441 caagcataat ttagttctcc agaatgacca ttgccacact ggttcttttc ctttcctatc
    49501 atttcaatag aagaatcctc ttcttacctt aatttctttc tcagtaacca atccgtatct
    49561 actttttgac caaccataaa atgatgttgg ctaaattttt caaaaccata tcggttataa
    49621 aaggcttgag cttttgtatt atgctcccaa acacctagcc aagcccaaga aaaactattt
    49681 tttgtagcaa gttcaagtgc gaattcaaac agttgcttac ctagtccaaa tccttggaat
    49741 ttttgtagca catagaggcg ttgaatttca aaagcgtcct ctaattctct ctcagtttga
    49801 gcacttcccc agttgacttt gagaaaacca gctatctcct cctcatgcat aatgaaatag
    49861 gtttcagagt caggatttcc caactcagtt gacacagttt tcagactata agcctcttca
    49921 aagtattcct gtaactgctc ttccgtatta tcatacgcaa aggtttcacg aaaggtttgt
    49981 ttggcaattt tagccaacac ctcaacatct gccatttcta cttttctaat cattatttaa
    50041 actgttctga gaagcggaca tctccttggt agaatccacg gatatcgttg attccataac
    50101 ggagcatagc tacacgctct tgtccaagac caaaggcaaa gccagagtat acagtcgcat
    50161 cgataccact catttcaagg acacgtgggt gaaccatacc ggcccccata atttcgatcc
    50221 aacctgtttt cttacataca ttacagcctt ctccaccaca cttgaagcaa gaaacatcca
    50281 cctcaacaga tggctctgtg aatgggaagt aagatggacg caaacgaatt tgacgctctt
    50341 caccaaacat tttttggaca atcaactgaa gcgttccttg aagatcagcc atagagatat
    50401 ttttcccaac taccaagcct tcgatttggt tgaattggtg actgtgggtc gcatcgtccg
    50461 tatcgcgacg gaagacacgc cctggcgaga tcatcttcaa aggaccttta gaaaaatcat
    50521 gggcatccat agcacgcgcc tgaactggag acgtgtgggt acggagcaag atttcttcag
    50581 tgatatagaa agtatcctgc atatcacgag ctgggtggtc ttttggaagg ttcatacgtt
    50641 caaagttata gtagtcttgc tccacttcaa aaccatccac gacttgataa cccataccga
    50701 tgaagatatc ttcgatttct tcactggttt gtgtcaaaac gtgacggtga ccagtcgcaa
    50761 ctggacgacc tggaagcgtc acatcgatac tctcgctagc cagttgagcc gcgactttct
    50821 tttcttccaa gagcttagct gtttcttcaa aagcagcagt caagacatca cgagcttcat
    50881 tgacgtgttt cccgatgatt ggacgcatct cagcagaaac atctttcatc cctttgagga
    50941 tttcagtgag cgaacccttt ttaccaagga cagagacacg caaatcttgc atctcttttt
    51001 catttccagc agtaatctgc ttcaagctag ccagcgtttc ttcgcgaagc gcttttaatt
    51061 gttcttcaat agttgacata tttcctccat cagtctctcg tagataaaaa gaaaaccaca
    51121 tgccaaaaac tccactcgga gcgttgacac gcggtaccat ccgttttcat ctgacaagtc
    51181 agaccttcat ttctaaatcc atgcgcaagt gaattcaccc agctttcata tagagagctt
    51241 gcagtcacgg ctctcctccc tgatatactt cccttgggct actagtcttt cagattccta
    51301 ttcaattact acttagttta tcagattttt accattcttg caagacctat cttacttctg
    51361 cttgttagct tattcttatc taactttata gcggattgaa ataagatgaa aacaaatcga
    51421 ttgggaaagt aaaattaatt tctataaatg ttttagcaat tgtttcgtac tattttagat
    51481 tcagtctgct atatataacc aatctatcct aagttaacta tttataatcg gctctctgta
    51541 acaaaattaa attaattaat ccccctataa aataaagagg tttagaattt gatgtcttcc
    51601 aaacctcttt atttcatatt taatgagatg ctgccttaac cgtgataata tctaatcatc
    51661 aataaacaaa ctatttgaat aaggattctc catttgattc aatcactcct ttataccaag
    51721 taaaggacat tttcttatat cgattgaatg tgccatttcc atcatcattt cgatcaacat
    51781 aaatgagacc gtacctctta gaaagctgtg cagtggacat agaaacgcag tcaatacatc
    51841 cccaagacgt atagcccata atttccacac catcttgtag agcttcagct acttgcaata
    51901 aatgttcttt catatactga attctataat catcttggac tgttaagtta ttaaattcat
    51961 cttttattag ttgatcttta gcacctaatc cgttttccac tataaataat ggaatttgat
    52021 aacggtcata atatctattt aaaattatac gtagtccaat tggatcaatt tgccatcccc
    52081 actcagaaga ctctaaataa ggatttacta aaccaccaat aatattccct tctcctgaat
    52141 tatactgtgt tggaagagca gattgagtta cactcatgta atagctaaag gataaaaatc
    52201 tacagtataa ttttttaata actctgcatc ttcagctgca aactctatgt taatgtcatg
    52261 ttccttaaaa tatctttttg cataattcgg ataataacct ctaacatgca catctgaaaa
    52321 tagataattt agattctcat actcatgagt cgcccataca tcttttggat ttggagtcat
    52381 tggataagct ggcatagcta ataccataca ccccacctta aactctgaat taatctcacg
    52441 agcaattttt gtaaccaaac tcgaggcgac taattcatga tgtatagctt gatataattc
    52501 ttgtttcgaa agattctcct taggtatatc tattcctcca ctagtaaacg gtaattccaa
    52561 aacagagttt acttcgttaa atgtaagcca atatttaact ttatctttat acctttctaa
    52621 aactgttcga gcaaattttt cataaaaatg aatcattctc ctatcaatcc atccatgata
    52681 ttttcttgct aaatataatg gagtctcata gtgtgaaaga gttacaagtg gttctatccc
    52741 gtgagcatgt agttcatcaa acaattcatc ataatatttc aacccagctt cgttaggttc
    52801 ttcctcatct ccttttggaa aaattctact ccatgcaata gaagtacgaa aaacattaaa
    52861 gcccatttca gaaaacaagg atatatcttc cttatattta tgataaaaat caatacctat
    52921 caattttaag ttatcttctg taggattttc tgttgcttct cctaatccac ctttgggtaa
    52981 cacatcctga actgataagc ccttaccatc ttcattatat gctccctcta cttgattagc
    53041 tgcaacagct ccaccccaaa gaaaatcatc tggaaaaatg gtcataactt tcctccatta
    53101 taatattacc agtaattcct tagaatgctc gattgtctga ttattaggta atattaatac
    53161 atctagaaaa tcattggtat tcgttacaat tactggtgta actgtttcgt agcctttagt
    53221 cttgattaaa ttcaagtcca tttcaaaaat caactgattt ttgaaaactc tgtctccttc
    53281 ttctacatga ctaataaaac cttgaccttt tagctcaaca gtatctaatc caatatgaat
    53341 tagtaactca acaccctcat cactcttcaa tccaattgcg tgcttagtcg gaaaaatatt
    53401 tgtaattttc ccatcaaatg gtgcataaac cttaccttca cttgggataa tcgctactcc
    53461 gtctccaatt agtttatctg aaaatgtttt atcctggaca tcgcttaacg gaatgatttc
    53521 tcctgatata ggagaaaata tcattttttt atttgaaact ccagctttaa cttctaaatt
    53581 gctagaactc tcttcttcat cgattccaaa tatataagct aatacaaagg taataacaac
    53641 cgaaataacc gccacaatta aagcatttac aatatttgat ggtacatcag aataaataaa
    53701 ttgaggcaac gctatcaaag atgggacagc aaatagatac gctttaacac tagtaagacc
    53761 tgcaaataat cccgctaatc caccaccaat catagctgca taaagcggtt ttttatattt
    53821 taaagtcaca ccatataatg caggttcggt aatccctgca agtaaggctg agaaacctgc
    53881 tgcaaaagca atttgttttg tattattatt tttactcttt aatgcaacag ccatcgaagc
    53941 agccccttga gctaagtttg accctaacat tgctggaaga attaatacgt ctggagtagc
    54001 aatagatgcc gccaaaaaaa taggtgcaaa agcccaatgc attccagtca taacaataaa
    54061 tggcataata gcaccaagaa tagctaatgt aagccatcca gctacaccat acatttgccc
    54121 aactagattt gataatcctt caccaacaat tactccaata ggtccgacta caactaaggc
    54181 aatacagctt gatactaata atactagcgt aggttgcaaa aaactcttag taacagctgg
    54241 tgttaattta gcaatcattt tttcaatata tttcatcaac caaaccataa taagaattgg
    54301 aacgactgac gaaccataac tagctggtgt cacaggtgca ccaaataaac taagaggatt
    54361 ccctgattgc accatttgaa caaaatttgg atggagaagt acacctgcta cagacatagc
    54421 taatgtagat gttactttta atttttgtga tgcagaataa gctaataaca gcggtaagaa
    54481 ataatatgga gcatccccaa aaaatgtcaa aaaagcaata gtctgagaat ctgattgcaa
    54541 tataccaagc attggtaaaa taattaccaa gactttcagc atacctcccc ctaacattgc
    54601 tggaatgatt ggagtcatgg aaccagcgat atattcaatg attctttcta aaatattccc
    54661 tttgtgccct tgaacaactg aatcggattc aaaattgcca agtttaacga attctttata
    54721 ataattagct acatcattac caagtataat ttgatattgt ccattctttt tcataatacc
    54781 tattacacct ggtatcttct tcacatcatc atcattgact aaattttcat cttttaattc
    54841 taatcttaaa cgtgttacac aatgggtaac tctattgaca tttttttcac ctccaattac
    54901 atcgaggatt ttttgtaccg tatctttata actcatggta ttctcctatt ctattaatct
    54961 aaattttttg ttaagcgacg aatatgagcc atcaaataaa ctaattcact agaagtcaac
    55021 aaataattgt actcagtttg tataaacatt gccacctgtt caccacattc atattctcta
    55081 ggatatttat ttttcattaa tgctaacaag tcttcatcat catcgtcaca ataagttgta
    55141 ttttcaacca agcgatgggc aaataatttt atatgagtta tgaatctata ataatcaata
    55201 gaatcttggt taaactcagt acaatagaaa tcttgaatga ttttctcaat tcccataatt
    55261 atttcagtga ttttatacga ctcttgaaaa ttattttcta aattagcatt aacaaaatgt
    55321 agcgctatga atgcagcttc gtcaattgga agttcaatac ctaactcttt ttttattatt
    55381 tgtaaagccc ttttcccaag ttcaaattct tctttataat atttcgagat ttcccagcgt
    55441 aggggattcg aaataataac tccttcctta taacgttcta tgctcgaaga aatatgatcc
    55501 gttaaattaa tatagataat ttcatcaaaa ttattaccca atgttatttt acctaggtta
    55561 attatttttt cggtgatgta caccaactcc aaaggaacgt taataaacat ctctgtaaaa
    55621 cgggtcatat tatctctatt tttgagaaaa aaacttttct caatatcaat tggatttact
    55681 ttatctccaa tttttcttcc aaaagctatt ccccttccaa aaagaagaat atcgatatct
    55741 tttttacttt gcgcaattac ggcattgtgg tttagtattc gttttatgat cataaaattt
    55801 cccttttaaa ataaaaggaa cctacttaaa aaacatcaag aaacttcttc tgatgtgatt
    55861 aaataggttc ctgctcactt gtattagtga taacatcctt ttatttcaat ttttaatggt
    55921 gacatgaagc ttaaataatc aataactctg taaccgtttt tgttttatga tatttcaatc
    55981 tatttgtcaa gtacttataa aaattttttt attttgtgtg attcgaatgt gataatggca
    56041 acagtctcac taaagaaatg aaaatcatac atagaaattt ttaaaaattt tctctatcgt
    56101 aaacctgcca ctagttcaga aactatacta gctgattcca aacgtaatat ggtcatctca
    56161 tctaccttcc ctaataatgg cattgcacca taccaaacaa tagtcttatt caccaaaaga
    56221 ataggcgtta ctatttttga ttgagtaaaa gacactttaa ttttctggct cttcaactgg
    56281 tctagccatg ctttatttgc aaccgtctct ggtatacata tctcaatttg actaatccta
    56341 aattctttta gaaaattcat cagttttgtc tggtgcacat aaggtaaaat aagcagacat
    56401 tcctgtcttt cccctgctaa gtcctctctc aacaccttct catatctact atcaacataa
    56461 acgaattgtt tctcctcacc ctcaatggca cgataatcca tctttcgata agctacttgt
    56521 cgtttctgaa acatcttttc taaataagga acatgaatat ccacataatc gaaaatccgc
    56581 accaaagact tatccttgta gtttctatga atccgacctg agtactgaat caaattattt
    56641 ttccaagaaa agggtgctgc caagataagc gtgtccaact gaggtaagtc aaaaccttcg
    56701 ccaatgtatt ttccagtaga caacaaaaca aaccctttat ctaactgttc taacgtctcc
    56761 agtaaactcg ttctctttct cggactttgg tttttccgct aataatgtaa cagtcatcaa
    56821 cctctttctc tttcaataac ttttcaaaga catctatctg ttgaattcga ttaactaaaa
    56881 ccaagatatt ccgtccttct gccacttggg ctagaatatc cttgagaatc aactgattcc
    56941 tcgctgagtc tgtagcaatc caatcactga gctgtataaa attacttgct ttggtctttt
    57001 caatttctct gaaaaacaat aggttcatga ccattcttac gctcaggcgt agccgtcaaa
    57061 ccgtaaagat acttccctct aaactgagca acaacttttt caaacatcaa ggcagagata
    57121 tgatgacact catccacaat catcatctca tactcatcca aaagactttg actattttcc
    57181 aacttaaata gagattgaat cataacaaca tcaaccagtt tactcagtca tttcttagcc
    57241 ccaccgtact gcccgacata gcctattacc ctttcacgac ctgatggtgt ataacgggta
    57301 gcttctgcct cttcgaaagt caaaaaacac tttaaacgat ccagtcattg gtctaagagt
    57361 tgtctattat gaactaggat aatggttttt gtcttacgtt cagagataag agcagcacct
    57421 aaaacgatct ttccaaaacc tgtcccgcat gaagtaaacc attttcttta gaattcatat
    57481 ctgacagggc taattcttgc tccaaagtaa gctctccctt aaatgctact ctaatagacc
    57541 tttgtacttt tctcctatcc tccacaacta cctgcttaaa tttatcttgc aatggatata
    57601 acaagcctct tggcagccat aaataataat cggattctcc aaataaatac attcgctcag
    57661 gaatttgata ggttggctgt cgcatagcct gctttaaata taataaaatg aaataataac
    57721 aggacaaatc gatcaggaaa gtcaaatcga tttctaacaa tgttttagaa gcagaggtat
    57781 actattctag tttcaatcta ctataaaatt cgggattaga aaaggaagcc atatttttca
    57841 agaaaaaaag tgccttggat gtcacagacg atttttcaag ttggatcata ttggataaaa
    57901 cgaccttcaa ctccttatcc aattcttgct tgcctaactc ctcttggatt aacagtgcca
    57961 ctttagcagt tgaaatcctc tgaatttctt gtagatacct ccattgatct tcataaggct
    58021 gaaaatgttc atccacaaag accgttcgcc cttgatgata agcttctcct tgaaaaggaa
    58081 gggaaattaa attatcaaat ccacctttag gaagaacatc ctgatttgga aacatgccat
    58141 caaaagaatc aaaggacagt tgcatacttt cctgcatagc caattctaat agtttctttc
    58201 caaacaggcg agcctttcga ctcagaatcg cttcctcaaa gaagaaccaa atatggagtc
    58261 cgtaacccga acgagaaatc tctaaatggg cttccatctg gcgttccctt gctattcttc
    58321 gaatggttaa accagcttct ttccaatctc cttcatccaa atccagtacc aaaaaataac
    58381 agctatcatc taagtgcata ggaaagatac cgatagctgc ctccccacga aaatgagatt
    58441 tcaaaacgga gttcgtcaat gtctggaaac tccgtttttc aggtggcaac tgcttccaac
    58501 cataatcata ggaaggaaaa tactgaattt tcccttgttc attgataaaa ttcttagcat
    58561 aaacatcata acgtccagca aacacggagg taaacaattg taatttttct tgcgtcgtca
    58621 agtgactact ttgaagttgg aacatctcct cgaaccgaga gcgttccatc acaaactgac
    58681 tcttgtcttt aagacgttta cagtggaatg aaataagata tgaacaaatt gattaggaaa
    58741 atcaaattaa tttatagaaa tcttttagca gtcatggcgt cctattccag cttcaaaatg
    58801 ctatacacaa gaaaaactca ctatccccat taaaagaaaa aaagacatca agtatttcta
    58861 ccacaattcc atccaaagac gcaaaaacag ccataagagt cacctccttg attcctatag
    58921 gctgattata acaagaatag ctgaaattgt acacgaaaat aaaatccgaa tagcactcat
    58981 tttgtatgcg actaatagtc catctcgctc cggaatgtac gaagtaaata gagtttaaac
    59041 gagcagtcta taaaattcaa aaaacgcata ttatcaagcg acctaaaact ttgatgatat
    59101 acgttttctt gtgagaatat ttacttcatt tttgcctaaa attcaatgtt tactcagtat
    59161 ttggattatg aaaaatcgag gtctaaatct agatacattt tttctgaaga caaatcattt
    59221 tgaccaccga gcaagagatt ttcaaaaaaa gctgttaaaa actcaggacg tcgctgtaaa
    59281 atctttgcat tatctaatac caaggcatca cgaaaatact tggaatgttg ctgaaatggt
    59341 atattatcaa tatcaaaacc aaactcacga agatactgaa tcaaaaagac cgttactgtc
    59401 cgagtgtttc cttcgcgaaa tggatgaatc tgccagattc ctgaaataaa atgctggatt
    59461 tgtttaacca catccgcctg agttagtgtc gcatatgcaa cttgtttttc ctgattaaaa
    59521 tcataatcca aggtcatttg aatcatggag taatcagagt acacaacact ttcaccattc
    59581 aaaacaggtt cattctttgt gatattggtc tgacgaaatt gccccaccgg aatagagggt
    59641 tcaaatatat cttgaaacaa ctccttatga atagcaagta aggtcgcagg actaaatcta
    59701 aaacctcttc gagacaagag ttctacgata cgcaaagaaa ccaagtctgc ctcctccgta
    59761 ctcgcatcaa cggtatgatg ataaaccgtt gcatcctcat aaacctgctc ataagtcagt
    59821 tcaccccggg actgtttctc agccaaagat tccatatacg ctgatggcac tagattgtca
    59881 actttctgca gaccaaaacc tatccgccat aaatcacgct tcgcttcata agacaagttt
    59941 ggattgtcaa tgttgtaagt tggttgcata gaaatatcct ttcaattgtt tttctgtgtc
    60001 atcggtaaat tgtgaaatag gtatggaatt ggcgaaaatt ataaaaatcc ttcacaatag
    60061 gttctatgaa atatatatac ttcatcttaa tagtgagcaa gctaaactta gcatttcatg
    60121 ccctcatatg ggatgttctt tgactaaata atatgattat cgagatatat ctggataaat
    60181 gaactaataa gtctgacgcg tagacttatc aaagtcattg gcatacacca ctatgaactc
    60241 gttggtctgt tcaaatccca acacattacc tgagaagaaa gttgcaatgt tgtttttggt
    60301 gcgggtttga atttaaaaaa tttgttatgt agtacctaat ctaaggaatt agaacaatgc
    60361 ctctaatttt tctttaatac actgaaacat tgatgattct ggctgtattt ttgaaacagc
    60421 tcttctttgc tcctggaaaa tatcttcaga agttatattc tctattccta acgctacttg
    60481 agtttttttt ctaaaatatt cttttccgtt gccatcttta gaaaaatcat aaccttccct
    60541 atctacgctg ttacacaaat tagctaaaaa aaactctggg gttgggaaag gaagataaga
    60601 aacgtattta gcccaataat ctataaagct tctttgtttc gcaattaatt caatattatt
    60661 tttttgccct ttattacctg aaacattaaa tttaattgga catcccgtta tcaattttat
    60721 aatatcatca agatttttat tatctgattc aggaatttta tctgatataa caacaccatt
    60781 ttcaagatag ttcattaaat tatttgattc actaacatta gtgttttgat ctccatcaag
    60841 ccaaaaataa tggttatcgg aatctaaata cgatgagttt aaaatattat tacaaattat
    60901 ttgatttgct ccaccaggaa tatatctcac tactaaattc tgtttaagat tctcactacc
    60961 tgaatgagtg ataacaaact ctagaatata tttagctagt ctatcttcaa cataaatcat
    61021 cttcctagaa tgatacacat cacctaattc aaaaaatgca tcctgataat caatattttc
    61081 aataacatct accttttctc cgtttttcac taaaagtttc acggcttctc taggaaaatc
    61141 ttttataagt tgtgtagaat gtgtagtgat aataatttga tgttttttat ttaaacactc
    61201 ttgaagtaaa aactctttaa atttatagat tgcactcgga tgaagtgaga tttcaggttc
    61261 atctattaat attaatgaat ttgattgcgc atttactata tcatttacta acaaaataat
    61321 tctagcctca cctgttcctg caaaagcctc ggaatattct tttccagatt ttttcatcca
    61381 aatagttttg gaagctttta tatcatcacc ttttgaatac aacttatgtg ttaaaatttg
    61441 aatgtctgta taagattcat ccattatttc actaataatt tcacaaactt tatcatcaac
    61501 tttaacatta tctataacca tttccttttt ataacgcgta tagctacttg tattattctt
    61561 taaaatatca gcaactggtt tagatcgtaa tcttataaaa tcttgtttac tacgttgagt
    61621 agaaattttt ttaaaattat agtgatagaa aaataaatca aaagcagaaa catattcttt
    61681 acaatcacaa aagacaacat ttttttcaat gccatcccat ctgtctgtcg aagaacttcc
    61741 aatatattta tttttgggta atctttccat ctcatattgt ttttgaggag catatggttc
    61801 ccaataatct aatccttttt ttgttccaga acggccttta agaacttcta catttctaga
    61861 agctttaatg ttataatatg aatagattaa acattgtttc ccatccactt catctatttg
    61921 atcaacattt gtactaaacc aatattcaga cacactttta ttggctggag aaccatataa
    61981 agcttgtaaa attgaagttt tatttactcc atttctacca actaataaag taatagggaa
    62041 attaaaattt attcttgaat ttaatgataa gctcttgtaa tgaggaaatc gtatataatc
    62101 aatataagga taaaattgat ttttattttt aaaaaaattt ttagttattc cttctaaact
    62161 ctcaattatc tgattgtcca tttgctatct ccctataaac tgacataaaa taatttccac
    62221 tagcttgagc taattttact ggaactgcat taccaatttg cttagctaat tgacctctat
    62281 ttccaacaaa aatatagtca tcatcaaaag actgtaatct tgcagcttct cttgcactta
    62341 aagctctatt ttgagttggg tgcccaaatc taccttttga atatgacata caccctcctg
    62401 taatagtaat ggaaggtttg ttgatatcta atattccata tacgtcacta tgacctaaac
    62461 gatttttatg acaatttaat tctaaagatt ttggcaaaga ggttctactt cccccattat
    62521 ttctaatatg agtaattctc tcaatattta aattagatag tttgtttgca acatgattgt
    62581 agataccttc tccaatatat tcttcaccac cttcaatagg aggcaagtcc attattatat
    62641 ccgcattaat ccatgaaaga aggtccaatt ctggtatatt tgaatgagtt ttggaaggca
    62701 aattcaaagt taaattcgtg ttatcaagaa tttcttttct aattccgtgt aacaccaatc
    62761 tttttctaga ttgaggaact ccataatcag ctgtatttaa aatgtcataa ataacactat
    62821 aattattatc gattgatgtc aaaaattttt taaagatttt ttcatttctt gttctagtca
    62881 tacccacaac attttccatt aatagaaaat caggattcaa ttcattaatc aaacgaagaa
    62941 attgaaaaac aagttgattc ctctcgtcat tttcattatt agtaccaata gttgaaaaac
    63001 cttgacatgg tgggcatgcc acgagcatta gtttatcgtc tgtatcaatt ttaagatact
    63061 cttttacttc atctcctgat actacattta tatctttatc tattacagac acctcaggat
    63121 tatttaactt ataagtttta acagctacgg aatcaatttc aacagcaact tgaacatcta
    63181 tgcctgattt tttaaggcca cttgtagtgc cacctgcacc gctaaataaa tcaatagcaa
    63241 caattttccc catagtattc tcctaaagtt tctccttttt attataacat tatcaaatgt
    63301 aaaacccaac ccgatagggt taggttttta acatcatttc accaacttct tcatctcatc
    63361 aatacgtgct acggtcgcat catacttggc ttggtagtca gcttgtttgt cgcgttcttt
    63421 ttggacgact tctggtttgg cgttggctac gaagcgttcg ttagagagtt tcttaccaac
    63481 catgtccagt tctttctgcc atttggcaag ttctttttcc agacgtgcca attcttcttc
    63541 gacattgagg agatcggcca gtggcaggta gatttctgct cctgtgatga cacttgacat
    63601 agccagttca ggtgcaggga tggttgatgc gatttccaag tgttctggat ttgtaaagcg
    63661 tttgatatag ttgacattgc tgttaaagaa ggcttccaag tcgctatcgc ttgtcttaac
    63721 aaggatggtg ataggtttgc tttgtgctac gtttacttcc gcacgcgcat tccgaacagc
    63781 acgaatcaag tttttgagac tttccacacc agtgtgagcc gcaaggtctt caaaggctag
    63841 attaacagtt gggtatgcag ctgtcacgat agaaccttct gagatttgtc caaagatttc
    63901 ctctgtcacg aatggcatga ttgggtgaag gagacgaagg atcttgtcca gagtgtaaag
    63961 gagaacagaa cgtgtgatga ctttctcttc ttcattatcg ctataaagga cttccttggt
    64021 caactcaaca taccagtccg caaattcatc ccagatgaag ttgtagagga tgtggccagc
    64081 aacaccaaac tcaaacttat caaagttagc agttgctttt ccaatcgttt cattgaggtt
    64141 gtggagaatc cagcggtccg tcacattacc agcctcacct gttgcaactt tttcgacatt
    64201 ggcagttgct tgctcaaggg gcaaaccttc attgttcata aggatgtagc gagagatgtt
    64261 ccagatcttg ttaatgaagt tccatgaagc atccattttc tcataagaga aacgcacgtc
    64321 ttgtcctggc gcagaaccgt ttgaaaggaa ccatcgaagg gcatcggcac cgtatttctc
    64381 gataacatcc attgggtcaa tcccgttacc gagagactta gacatcttgc gtccttgctc
    64441 gtcacgaata agaccgtgga taaggacgtt ttggaatggt tgacggcctg tgaattccaa
    64501 tgactggaag atcatacgag acacccagaa gaagatgatg tcgtaacctg ttaccaaggt
    64561 tgaagttggg aaataacgtt taaagtcttc tgagtcgact tcaggccagc ccatggttga
    64621 aaatggccaa agagcagaac taaaccacgt atcaagcaca tctgaatcct gagtccagtc
    64681 gtctccttct ggtgcctctt ctcccacata aatctcacct gcctcgttgt accaagcagg
    64741 gatttggtga ccccaccaga gctgacgaga gataacccag tcgtggacat tttccatcca
    64801 ttgaaggaag gtatcgttga aacgaggtgg gtagaattcg accttgtcct ctgtgtcttg
    64861 gttggccatg gcgttcttag ccaattggtc catcttgacg aaccattgag tagacaagcg
    64921 tggctcaact acgacacctg tacgttctga gtgaccaaca ctgtggacac gtttttcgat
    64981 tttgacaagg gcaccgattt cttccaactt agcaacgact gccttacgag cttcaaaacg
    65041 atccatgcct gaaaattcaa aggcaagctc attcatagtt ccgtcgtcgt tcatgacgtt
    65101 gacttgtggc aagttatgac gttggccaac caagaagtca tttggatcgt gggcaggtgt
    65161 gattttcacg acaccagtac caaactcagg atctgcgtgc tcatctccaa cgattgggat
    65221 gagtttatta gcgattggaa ggatgacgtt tttaccaatc aagtccttgt aacgcgggtc
    65281 ttctggatta accgcaaccg caacgtcccc aaacatagtc tcaggacgag ttgtagcaac
    65341 ttcaagggcg cgtgaaccat cttccagcat gtagttcatg tggtagaagg caccttcgac
    65401 atccttgtga atcacctcaa tatcagaaag ggctgtgcga gctgctgggt cccagttgat
    65461 gataaactca ccacgataga tccagccttt cttgtaaagg tccacaaaga ccttacgaac
    65521 agcttttgac aaaccttcgt caagagtgaa acgctcacga gaatagtcta cagaaagccc
    65581 catcttgccc cattgttcct tgatggtagt ggcatattcg tctttccatt cccagacctt
    65641 cgtcaagaaa gactcacgac ctaggtcata acgcgtaata ccctcaccac gtaagcgctc
    65701 ctcaacctta gcctgagtcg caataccagc gtggtccata cctggaagcc aaagcgtatc
    65761 aaagccctgc atgcgttttt gacggatgat gatatcctgc aaagtcgtat cccaagcgtg
    65821 accaaggtga agtttcccag ttacgtttgg tggtggaatc acgattgaat aaggcttagc
    65881 cttttgatcg cctgaaggct tgaaaacatc cgcatcaagc catttttggt aacgaccagc
    65941 ctcaacctcg gctggattgt atttagatga aagttcttta gacatgtgtg tgtcctttct
    66001 ctattttgtt tattttattt tgaatctgct tagcagcttc ttctgcagac aaattcgtat
    66061 tatttatttt aaagtagtgg ttcaactcat tcggttgatg ttgggaattt aattgaagtg
    66121 tttcagcggt ctctataatt tctctttcag atacctcaat atgtcgtttt aagggtttgt
    66181 actttaatcg attctccgtt cgatttcgac gtatgcactc ttcaagactt gtttccaact
    66241 caacaaaaag aatctcttga tgataatcat ccaacaaact ctgaatttgc ttgaaatata
    66301 tcagatgata ctgatttgaa aaatcaatta catctgttaa aattactgat cgctgatttc
    66361 ttgcactcgc tccaaggaat gaaaaagtga ttccacgaac aaattcccac atttcctctg
    66421 tatagtcctg atagatttct agtgcaaaat cgatggcttg atggttataa aagagggtag
    66481 aatccatcag tcgagacagt tcttgaccaa tggtcatttt tcctgatgct ggagcaccaa
    66541 tgatgaaaag atgcatcaaa tcacctccca ctcactctcc agcaaggcat atctcaaatc
    66601 atcacagcgg ttgccttgag catctttgcg gtctcttatg cgagcttcga gggtaaagcc
    66661 aagcttttcc gcgactcgtt tactttgaag gttatatcca aagcaagtta gttcaatctt
    66721 gtgaagaccc aaatctttaa aggctaagtc aatcaaggca cgcgcagctt ctggcacata
    66781 acctcgcccc caatagtctg ggtgtaaggt atagccgatt tccagcacat catcttcatg
    66841 gcgatggttg aaatcgacag agccaacgat cttatccgtt actttgacga caatcccgta
    66901 gcctgctggg agattttcct tttgattacg ctccggaaga atgtgctcca gataataaat
    66961 ctcatcttcc aaggtcttga ctggaggaaa acctgctgga taggcgacct ctggcaaact
    67021 agcatagtca aagatatcct cagcatccgc cacagtacgg actcgtaaaa cgagacgttc
    67081 tgtttcgatt ttatctggca gctcagttct tgccatcctt cttcctcgct tttttgatga
    67141 aactgccctt catatctaca cgcttatcca gatagcgata aacgcgctga tatccatctc
    67201 ccatgaaata ggttggggca aacagttgat ttttaaaatg tcccttttca tccaggagtt
    67261 ctggggcaac aagtcgctca agaatcttgg caaagatgtg gcaaataccg tcttcctcaa
    67321 caatcctatc tacccgacaa tctaaaacaa gtggacaggc gtctaaaata ggaatctgag
    67381 ttcgttcaga aatttcataa tgcactccca aacgttccaa tttctcctga tgactgataa
    67441 aaccagcctg ctccatcgca agcatagaag tttcatcaga aatattcaca gtaaattttt
    67501 gatactgttt gatctgctct gcggcattct ctctcgcaac gactccaatc acaacccaat
    67561 ctcctagact ataagaggaa ctacaggtcg tgatgttata gccaaaattc taatcttgat
    67621 atcctaaaat aaaaacagga aaaccataat atagtttact tgtgttaaaa gattgcttca
    67681 taacaacccc ctttgactaa gacgtaaaag aaaagccctg ccatctacat gacagggacg
    67741 aatgtgttta tccgcggtac cacccaattt cgggcagttg cccgcaactc tcgtctttaa
    67801 agcaaaaaga cacttttatt tcaatttttc atccattagc aaccagtttt gacacatttg
    67861 tggacttctc agcgccgcca ctttctgtaa aatgtgggat tcaaaacacc tctaatagct
    67921 ctattgtagc aagatttttt cggaaatgca aggctcaaga aaaattactt gaacttgatg
    67981 ccattttctt tcaatttctt gatggtagct gggtcgattc ctttcaagtc aaggagttct
    68041 ttttcagtcc agtttttgaa atctgaagca gacttgattc cttcatcata gaaagtcttg
    68101 gcacgatcaa gtggaagtcc acccaagttt gctgcaaaat cttctacaga attagctact
    68161 ttttgagctt gctctttggt agcttctact gcttgttcaa cttttttaga aacagcctta
    68221 ccagctgcgc ttgctgactg ttctgcacgc ttcacgactt tttttgtctc atctacaacc
    68281 ttagtcacag tacttgaaaa ggcacctgcg cgacggaggc catttcgtaa ttgtttttta
    68341 cgattgagtt tctttgacat gataaaatcc tttcaataaa aaatccctgc tctgacaagg
    68401 atttaacata aacattctat caagatttta gtgaaaaatc aagaatcttt ctcgtttaat
    68461 aatttcgctc accaaacaag ccttctggca aatcatggaa tcccttgcct actttgaagt
    68521 tttcaaactt accaagcaag aactgataag catccaaacg actctcgtag cgaatcatgg
    68581 catctttggc acgctcgtcc tggtcttctt cgtaaacttt ttgactttcc ttgtgcgcca
    68641 tgtcgttaat catgatttgg gtattgaccc aagcttcaaa ttccttcata aattcttgtt
    68701 cgtaactcat tttttctcct tattctaatc cacggaaata gtccgtatca atctcacggt
    68761 agggctggat atcctgaacg tattccagta caccttggaa ttctccgttt tcatcgtgta
    68821 ctgcagcata ggtgatgtgg acaaactttc ctcgcgactc agacttgaac cacatttcat
    68881 acttgtcctt ggtcccctca cgaagtccct tcatgatagc cttgaccttg tccaaatact
    68941 taggcggatg acagagttcg acattgcgcc cgacttggga cggcgtccgt ttgaaaatca
    69001 tctcatcagc tggcgtattg tcattgtaat actggaaaat atcttcttta ttgacaaagg
    69061 taatctccat agggagatga ttgaggatga gattggcctg ctcgactgaa agatagccat
    69121 tcccaaaagc ctgttgacta tggcggtcca gcacagcttc cttttcctta ggggtaaagg
    69181 taatggtaaa atggccttct ggcgtatcta tgacttgttg aacttgacct tctgccgtat
    69241 ctagttgtac aggctcctct gcaatctttt cctcaataaa gatctgtcgt tctggcaccc
    69301 atttctctga cggacggatg atggcatagc cataggcatc actctcctcc gcaatctgaa
    69361 gccagtcatc ctgagtaaaa gactcaagga gaatcatgag caggatggac tcttccttga
    69421 aaatcatact ttcaaactct gtcgcaaaag cttcaaaagc ttcctttaca ctgctaattg
    69481 acacttctgg tagtgacttg gctgtcgtta gagctgtttg aaagagttcc ctaatctgat
    69541 catccactcc ccacataact ttgggaggtg aatcgtgtcc atagcgctcc atgataggaa
    69601 agaagagttc ttccttacgt tggtaatgga tgtcaaattg acccacaagt cccatctgac
    69661 gcaccaaacc cttacgcatc tccgccagca tttcctcgtc ttccatagac tcataggtat
    69721 ctaacaatct acgaatgcga atcaaggccg cacggagagc cagattttct tctttgaaga
    69781 cacgaactgg gtgacctgga tgctcagtat ctgaaacttc gacacctttg atagcatttt
    69841 taaaaagatt ggcatggaca tcacagagtt ccataacatc ttcaaaagtg acgcccgaat
    69901 ccgagttcat cagctcgtgc tccataaggg aaatctcgat ggctgacacg cccgtaaagg
    69961 tcgcatcaaa gcgatcttga accgactcag gagaggcgcc attgtgcaat tctaacaaaa
    70021 tatcccgtag gatatgaatc cgttcatctg tcattagtct aatccaatca cttcgtagcc
    70081 attcgcttcc agtgtgcgta caatcttgtc cataggagtt cctgctagct tagaaccctg
    70141 tttaagtgat actttacgac caactgtatt gcgcattaag ggattggcaa ggggtttaaa
    70201 acccaactcc actagaattt ccaagacttc tggatgcttg tccaccactt ctgcaacagg
    70261 aattgacaca tcgatgatat tgtccatgac gacctccatt gtatgttcta atactcaatg
    70321 aaaatcaaag agcaaactag gaagctagcc gcaggttgct caaaacagtg ttttgaggtt
    70381 gtagatagaa ctgacgaagt cagctcaaaa caccgttttg aggttgtgga tagaactgac
    70441 gaagtcagta acatatatac tgcaaggtga agctgacgtg gtttgaagag attttcgaag
    70501 agtataaaat gattttactg cttatattat accatatgga aagagaataa aaaaactagc
    70561 agtggagttc ctgctagttc tctgcaactt ggattaccat ctagataagt ttaaagagcc
    70621 aatccaatat cttaaacact atcttgtaaa acagcaattg gactgcaaaa gagatgatca
    70681 tccaaaagaa tttcacaatc ccaataatcg gtttgataaa aagaatagca agaagacccc
    70741 agaaaaattt cattctctct cctctggctt aatgagccac cgagccgtat ccattaactt
    70801 gtcggcaata aaaagtttcc ccagaccgac aacggcatta tcatctttct ttatcgtttt
    70861 cattactttt acaccttgca cggtcaaaaa gtaattacca taaattttag ctagagtttt
    70921 tatgagtccc atattactct ccttcgatga tttcagcctc ttttcggatt gtttcaacat
    70981 cttcttgata ttgaacttca ctatagttaa ctagcttgga caattctttc tgcaagcttt
    71041 cacccatcgg tttcatagtt gcaaaggtta aaccacctga aatgatacca cccaagatag
    71101 gaataaattt ccccattcct ttggccagcc ctcccttggt aagattcaca ccaaatattc
    71161 ttaagacttt tttcaaaata ggataccaaa gcgtctttgt taaagcttta ttaggtactg
    71221 ttttcattac ctgtttggca attgttatac caccagcacg tagcaaagca gcagttccat
    71281 tcacccccaa catgacgcct agataaagca aaagggtatt ttgagcatct tcactcaact
    71341 catcgcgtga agcccaaaga tcctcataac cataaatata acctaattct tgggccaatt
    71401 tcagagagaa accataaaat tgagccacat cagctggaat ggtaattgcc atagcaagcc
    71461 ctccaggtaa tcctgccaaa acagaagtcc cactcgctaa taaaacatta tccctaatac
    71521 aggcattagc cactcgatct aagatttctt gagataagag agacgttgga ccttgttcca
    71581 ataaggtagg aatgtcctgt ggacccaatt ctttggaaaa cttatccact aaaaatttcg
    71641 aacgatcaac cttaacaact ggtagtttta ccacttgttg taatacttgc atagctaagt
    71701 cttgttcagc catatacaaa ctccttattt cttttttaca ccttatcata ttatatttcc
    71761 tacaaaaaaa gcattttcta attagataac attaaaaaat acagaacccc tttt
//
LOCUS       DBERMR010000004        70150 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.004, whole genome shotgun sequence.
ACCESSION   DBERMR010000004 DBERMR010000000
VERSION     DBERMR010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 70150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70150
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.004"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(4..300)
                     /locus_tag="ACL8A1_000290"
     CDS             complement(4..300)
                     /locus_tag="ACL8A1_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201138.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYKLTEYSKMK"
     gene            complement(284..2443)
                     /gene="pfbA"
                     /locus_tag="ACL8A1_000291"
     CDS             complement(284..2443)
                     /gene="pfbA"
                     /locus_tag="ACL8A1_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HGS1201139.1"
                     /translation="MLKIVKKLEVLMKYFVPNEVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEVVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKILNNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVIN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYI
                     SFIKSNANGKEPVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQYNSNNVI
                     DSNQKNGEFNSSKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVK
                     QKLPKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            complement(2723..2953)
                     /locus_tag="ACL8A1_000292"
     CDS             complement(2723..2953)
                     /locus_tag="ACL8A1_000292"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201140.1"
                     /translation="MSDIKVNIPGECLYDKVFVLSFIIYNISIYLNIVNGIFYIQAKK
                     DSITFEWKAKEQTRKLAIDSSKPCFEVVDIVK"
     gene            complement(3573..4799)
                     /locus_tag="ACL8A1_000293"
     CDS             complement(3573..4799)
                     /locus_tag="ACL8A1_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HGS1201141.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVEFAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(4842..5534)
                     /locus_tag="ACL8A1_000294"
     CDS             complement(4842..5534)
                     /locus_tag="ACL8A1_000294"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HGS1201142.1"
                     /translation="MLKWEDLPMEMKSSEVESYYQLVSKRKGSLIFKRCLDWFLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            complement(5707..7458)
                     /locus_tag="ACL8A1_000295"
     CDS             complement(5707..7458)
                     /locus_tag="ACL8A1_000295"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1201143.1"
                     /translation="MQNKQEQWTVLKRLMSYLKPYGLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLAYANRSVALDALFLRPAMSLLKLLGYAVLMTYFGYRGLSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALIDERTYEPLQENGQA
                     KVKEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLALGG
                     TYHKMYSLQAGAMTYTL"
     gene            complement(7448..9193)
                     /locus_tag="ACL8A1_000296"
     CDS             complement(7448..9193)
                     /locus_tag="ACL8A1_000296"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1201144.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALILVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLVVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADLILVLQNGQIIERGRHEDLLALDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(9399..9776)
                     /locus_tag="ACL8A1_000297"
     CDS             complement(9399..9776)
                     /locus_tag="ACL8A1_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HGS1201145.1"
                     /translation="MKRLIDGKPTLLIKNGNIDPEACRSVGLSASDVSLKLRSQGIFQ
                     MKQVKRAVQEQNGQLIVVQMGDENPKYPVVTDGVIQVDVLESIGRSEEWLLDNLSKQG
                     HDNVANIFIAEYDKGAVTVVTYE"
     gene            complement(9848..10304)
                     /locus_tag="ACL8A1_000298"
                     /pseudo
     CDS             complement(9848..10304)
                     /locus_tag="ACL8A1_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            10438..11265
                     /locus_tag="ACL8A1_000299"
     CDS             10438..11265
                     /locus_tag="ACL8A1_000299"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HGS1201146.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDAKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            11277..11372
                     /locus_tag="ACL8A1_000300"
                     /pseudo
     CDS             11277..11372
                     /locus_tag="ACL8A1_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
     gene            11420..11659
                     /locus_tag="ACL8A1_000301"
     CDS             11420..11659
                     /locus_tag="ACL8A1_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HGS1201147.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVPKLYQKVMEANYPI
                     YRLLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     regulatory      12042..12137
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            12537..13118
                     /locus_tag="ACL8A1_000302"
     CDS             12537..13118
                     /locus_tag="ACL8A1_000302"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HGS1201148.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMQEIGKVFAE
                     KFAATGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            13118..14380
                     /locus_tag="ACL8A1_000303"
     CDS             13118..14380
                     /locus_tag="ACL8A1_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HGS1201149.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LSAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLFAIVLNAVLNHKKK"
     gene            complement(14467..15333)
                     /locus_tag="ACL8A1_000304"
     CDS             complement(14467..15333)
                     /locus_tag="ACL8A1_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HGS1201150.1"
                     /translation="MKQTRNFDEWLSTMTDTVADWTYYTDFPKVYKNVSSIKVALNIM
                     NSLIGSKNIQEDFLDLYQNYPEILKVVPLLIAKRLRDTIIVKDPIKDFYFDFSKRNYS
                     IEEYTMFLEKSGIFDLLQNHLVSNLVDYVTGVEVGMDTNGRKNRTGDAMENIVQSYLE
                     AEGYILGENLFKEIEQNEIEEIFSVDLSAITNDGNTVKRFDFVIKNEQVLYLIEVNFY
                     SGSGSKLNETARSYKMIAEEIKAIPNVEFMWITDGQGWYKAKNNLRETFDILPFLYNI
                     NDLEHNILKNLK"
     gene            complement(15320..16090)
                     /locus_tag="ACL8A1_000305"
     CDS             complement(15320..16090)
                     /locus_tag="ACL8A1_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HGS1201151.1"
                     /translation="MTKPYYNKNKMILVHSDTFKFLSKMKPESMDMIFADPPYFLSNG
                     GISNSGGQVVSVDKGDWDKISSFEEKHEFNRKWIRLAKEVLKPNGTVWISGSLHNIYS
                     VGMALEQEGFKILNNITWQKTNPAPNLSCRYFTHSTETILWARKNDKKARHYYNYDLM
                     KELNDGKQMKDVWTGSLTKKVEKWAGKHPTQKPEYLLERIILASTKEGDYILDPFVGS
                     GTTGVVAKRLGRRFIGIDAEKEYLKIARKRLEAENETN"
     gene            complement(16116..16970)
                     /locus_tag="ACL8A1_000306"
     CDS             complement(16116..16970)
                     /locus_tag="ACL8A1_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HGS1201152.1"
                     /translation="MKIKEIKKVTLQPFTKWTGGKRQLLPVIRELMPKTYNRYFEPFV
                     GGGALFFDLAPKDAVINDFNAELINCYQQIKDNPQELIEILKVHQEYNSKEYYLDLRS
                     ADRDERIDMMSEVQRAARILYMLRVNFNGLYRVNSKNQFNVPYGRYKNPKIVDEELIS
                     AISVYINNNQLEIKVGDFEKAIVDVRTGDFVYFDPPYIPLSETSAFTSYTHEGFSFAD
                     QVRLRDAFKRLSDTGAYVMLSNSSSALVEELYKDFNIHYVEATRTNGAKSSSRGKISE
                     IIVTNYEK"
     gene            complement(17127..17531)
                     /locus_tag="ACL8A1_000307"
     CDS             complement(17127..17531)
                     /locus_tag="ACL8A1_000307"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HGS1201153.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERQVRI"
     gene            complement(17601..19082)
                     /locus_tag="ACL8A1_000308"
     CDS             complement(17601..19082)
                     /locus_tag="ACL8A1_000308"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HGS1201154.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYAYSPE
                     QAIEDFYQLSRKNDYIKLKAIAKNIAYRIPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFVRVLEDAGVYKQTEQGQTAFMRFVEHVGISLD"
     gene            complement(19101..20279)
                     /locus_tag="ACL8A1_000309"
     CDS             complement(19101..20279)
                     /locus_tag="ACL8A1_000309"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HGS1201155.1"
                     /translation="MTQHLTAETLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            20443..21450
                     /gene="galR"
                     /locus_tag="ACL8A1_000310"
     CDS             20443..21450
                     /gene="galR"
                     /locus_tag="ACL8A1_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HGS1201156.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFKNYSQARGIYHDELVFQGR
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALRALQEAGINLPDRVSLISYND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(21497..22534)
                     /locus_tag="ACL8A1_000311"
     CDS             complement(21497..22534)
                     /locus_tag="ACL8A1_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGS1201157.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEVAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNIFVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(22535..22888)
                     /gene="nmlR"
                     /locus_tag="ACL8A1_000312"
     CDS             complement(22535..22888)
                     /gene="nmlR"
                     /locus_tag="ACL8A1_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stress response transcriptional regulator NmlR"
                     /protein_id="HGS1201158.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSACVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(23132..24022)
                     /gene="czcD"
                     /locus_tag="ACL8A1_000313"
     CDS             complement(23132..24022)
                     /gene="czcD"
                     /locus_tag="ACL8A1_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter
                     CzcD"
                     /protein_id="HGS1201159.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGMSAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEYMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            24155..24691
                     /locus_tag="ACL8A1_000314"
     CDS             24155..24691
                     /locus_tag="ACL8A1_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGS1201160.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFQKNQDHIASLLFS
                     KNDYFLRQLQRELEHHVYPMLAADLKQTHPSLPTSYLQHFVVTNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            complement(24858..25664)
                     /gene="pnuC"
                     /locus_tag="ACL8A1_000315"
     CDS             complement(24858..25664)
                     /gene="pnuC"
                     /locus_tag="ACL8A1_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HGS1201161.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(26009..27529)
                     /locus_tag="ACL8A1_000316"
     CDS             complement(26009..27529)
                     /locus_tag="ACL8A1_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HGS1201162.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLVQKEKENLIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            complement(27522..28250)
                     /locus_tag="ACL8A1_000317"
     CDS             complement(27522..28250)
                     /locus_tag="ACL8A1_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS1201163.1"
                     /translation="MIEYKNVALCYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKVPATDFVADLFGGSVHD"
     gene            complement(28264..29028)
                     /locus_tag="ACL8A1_000318"
     CDS             complement(28264..29028)
                     /locus_tag="ACL8A1_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HGS1201164.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHDIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLKNGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(29032..29475)
                     /locus_tag="ACL8A1_000319"
     CDS             complement(29032..29475)
                     /locus_tag="ACL8A1_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HGS1201165.1"
                     /translation="MKDGHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKSQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            complement(30157..30453)
                     /locus_tag="ACL8A1_000320"
     CDS             complement(30157..30453)
                     /locus_tag="ACL8A1_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HGS1201166.1"
                     /translation="MVEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLASFIKREEAKYRGTATSALMYEELFKML"
     gene            complement(30475..31539)
                     /gene="pepA"
                     /locus_tag="ACL8A1_000321"
     CDS             complement(30475..31539)
                     /gene="pepA"
                     /locus_tag="ACL8A1_000321"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HGS1201167.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKLDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            31940..32641
                     /gene="galE"
                     /locus_tag="ACL8A1_000322"
     CDS             31940..32641
                     /gene="galE"
                     /locus_tag="ACL8A1_000322"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HGS1201168.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYQVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNASGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            32657..32767
                     /locus_tag="ACL8A1_000323"
     CDS             32657..32767
                     /locus_tag="ACL8A1_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="HGS1201169.1"
                     /translation="MNYLFQEDSSSQIFNLGTKKGYTIKEIFKTAEELLN"
     gene            33064..34023
                     /locus_tag="ACL8A1_000324"
     CDS             33064..34023
                     /locus_tag="ACL8A1_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1201170.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            34013..34969
                     /locus_tag="ACL8A1_000325"
     CDS             34013..34969
                     /locus_tag="ACL8A1_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HGS1201171.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSFFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRARQ"
     gene            34966..35718
                     /locus_tag="ACL8A1_000326"
     CDS             34966..35718
                     /locus_tag="ACL8A1_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1201172.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            35814..36779
                     /locus_tag="ACL8A1_000327"
     CDS             35814..36779
                     /locus_tag="ACL8A1_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS1201173.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEITI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(37004..37246)
                     /gene="yidD"
                     /locus_tag="ACL8A1_000328"
     CDS             complement(37004..37246)
                     /gene="yidD"
                     /locus_tag="ACL8A1_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HGS1201174.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(37246..37968)
                     /locus_tag="ACL8A1_000329"
     CDS             complement(37246..37968)
                     /locus_tag="ACL8A1_000329"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HGS1201175.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            complement(37955..38524)
                     /gene="scpB"
                     /locus_tag="ACL8A1_000330"
     CDS             complement(37955..38524)
                     /gene="scpB"
                     /locus_tag="ACL8A1_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HGS1201176.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            complement(38543..39271)
                     /locus_tag="ACL8A1_000331"
     CDS             complement(38543..39271)
                     /locus_tag="ACL8A1_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HGS1201177.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLVGRAQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            complement(39271..40005)
                     /gene="xerD"
                     /locus_tag="ACL8A1_000332"
     CDS             complement(39271..40005)
                     /gene="xerD"
                     /locus_tag="ACL8A1_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HGS1201178.1"
                     /translation="MRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETSLK
                     IYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEILYL
                     DSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVTIP
                     TALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFILRQ
                     IENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            complement(40002..40463)
                     /gene="cbpB"
                     /locus_tag="ACL8A1_000333"
     CDS             complement(40002..40463)
                     /gene="cbpB"
                     /locus_tag="ACL8A1_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HGS1201179.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            complement(40460..40981)
                     /locus_tag="ACL8A1_000334"
     CDS             complement(40460..40981)
                     /locus_tag="ACL8A1_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HGS1201180.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPY
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWMEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            complement(40957..41928)
                     /locus_tag="ACL8A1_000335"
     CDS             complement(40957..41928)
                     /locus_tag="ACL8A1_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HGS1201181.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKLSL"
     gene            complement(41925..42719)
                     /gene="racE"
                     /locus_tag="ACL8A1_000336"
     CDS             complement(41925..42719)
                     /gene="racE"
                     /locus_tag="ACL8A1_000336"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HGS1201182.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            complement(42926..43174)
                     /locus_tag="ACL8A1_000337"
     CDS             complement(42926..43174)
                     /locus_tag="ACL8A1_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HGS1201183.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            complement(43430..45055)
                     /gene="treC"
                     /locus_tag="ACL8A1_000338"
     CDS             complement(43430..45055)
                     /gene="treC"
                     /locus_tag="ACL8A1_000338"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HGS1201184.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNPNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKDINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            complement(45233..47200)
                     /gene="treP"
                     /locus_tag="ACL8A1_000339"
     CDS             complement(45233..47200)
                     /gene="treP"
                     /locus_tag="ACL8A1_000339"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HGS1201185.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLRDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEATKSAAKSNQNVV
                     QGVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKIEGDTNLQAEFVAQEEAEFVSHEPVELTSVEII
                     SPLTGQVKELSQATDPVFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVIVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATITGTYPTTIQAGASLMVATRI"
     gene            47385..48095
                     /gene="treR"
                     /locus_tag="ACL8A1_000340"
     CDS             47385..48095
                     /gene="treR"
                     /locus_tag="ACL8A1_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HGS1201186.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            complement(48128..48931)
                     /locus_tag="ACL8A1_000341"
     CDS             complement(48128..48931)
                     /locus_tag="ACL8A1_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1201187.1"
                     /translation="MASLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFISVICGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKHLNVRKRANDIARVFQDPKMGTASRLTI
                     EENMAIALRCEQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMAALVKPKLLLLDEHTAALDLKTSQMVMNLTQKIVEHHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(48944..49821)
                     /locus_tag="ACL8A1_000342"
                     /pseudo
     CDS             complement(48944..49821)
                     /locus_tag="ACL8A1_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(49833..50770)
                     /locus_tag="ACL8A1_000343"
                     /pseudo
     CDS             complement(49833..50770)
                     /locus_tag="ACL8A1_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512061.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(51261..52187)
                     /locus_tag="ACL8A1_000344"
     CDS             complement(51261..52187)
                     /locus_tag="ACL8A1_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS1201188.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            complement(52198..53265)
                     /locus_tag="ACL8A1_000345"
     CDS             complement(52198..53265)
                     /locus_tag="ACL8A1_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1201189.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            complement(53274..54200)
                     /gene="oppC"
                     /locus_tag="ACL8A1_000346"
     CDS             complement(53274..54200)
                     /gene="oppC"
                     /locus_tag="ACL8A1_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HGS1201190.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            complement(54200..55696)
                     /locus_tag="ACL8A1_000347"
     CDS             complement(54200..55696)
                     /locus_tag="ACL8A1_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1201191.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGTLTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPVTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            complement(55763..57742)
                     /locus_tag="ACL8A1_000348"
     CDS             complement(55763..57742)
                     /locus_tag="ACL8A1_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS1201192.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            complement(58428..59621)
                     /locus_tag="ACL8A1_000349"
     CDS             complement(58428..59621)
                     /locus_tag="ACL8A1_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HGS1201193.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            complement(59759..61201)
                     /gene="gtfA"
                     /locus_tag="ACL8A1_000350"
     CDS             complement(59759..61201)
                     /gene="gtfA"
                     /locus_tag="ACL8A1_000350"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HGS1201194.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGERYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFTLPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVVEIDLQNQTYRVIENGVEVSF"
     gene            complement(61512..62345)
                     /locus_tag="ACL8A1_000351"
     CDS             complement(61512..62345)
                     /locus_tag="ACL8A1_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS1201195.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     LFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQCHIISGMSNGAV
                     K"
     gene            complement(62359..63225)
                     /locus_tag="ACL8A1_000352"
     CDS             complement(62359..63225)
                     /locus_tag="ACL8A1_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS1201196.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSMVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            complement(63239..64498)
                     /locus_tag="ACL8A1_000353"
     CDS             complement(63239..64498)
                     /locus_tag="ACL8A1_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGS1201197.1"
                     /translation="MEWYKKIGLLATTGLALVGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            complement(64533..66695)
                     /locus_tag="ACL8A1_000354"
     CDS             complement(64533..66695)
                     /locus_tag="ACL8A1_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HGS1201198.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSC
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQIEKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRRHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHDIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMMRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGLVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTVSNEAAVQFNYENQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEIYSGAELMYAGLTVILSQGDFLSRQYIFRRL"
     gene            66803..67663
                     /locus_tag="ACL8A1_000355"
     CDS             66803..67663
                     /locus_tag="ACL8A1_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGS1201199.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFHYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELSQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYIRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            67660..68595
                     /gene="birA"
                     /locus_tag="ACL8A1_000356"
     CDS             67660..68595
                     /gene="birA"
                     /locus_tag="ACL8A1_000356"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HGS1201200.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLIQCDNGKEIWLNSGEISLNSWK"
     gene            complement(68721..68808)
                     /locus_tag="ACL8A1_000357"
     tRNA            complement(68721..68808)
                     /locus_tag="ACL8A1_000357"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(68772..68774),aa:Ser,seq:gct)
     gene            complement(68815..68888)
                     /locus_tag="ACL8A1_000358"
     tRNA            complement(68815..68888)
                     /locus_tag="ACL8A1_000358"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(68852..68854),aa:Ile,seq:gat)
     gene            complement(68924..68994)
                     /locus_tag="ACL8A1_000359"
     tRNA            complement(68924..68994)
                     /locus_tag="ACL8A1_000359"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(68960..68962),aa:Gly,seq:tcc)
     gene            complement(69014..69086)
                     /locus_tag="ACL8A1_000360"
     tRNA            complement(69014..69086)
                     /locus_tag="ACL8A1_000360"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(69051..69053),aa:Phe,seq:gaa)
     gene            complement(69090..69163)
                     /locus_tag="ACL8A1_000361"
     tRNA            complement(69090..69163)
                     /locus_tag="ACL8A1_000361"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(69127..69129),aa:Met,seq:cat)
     gene            complement(69177..69266)
                     /locus_tag="ACL8A1_000362"
     tRNA            complement(69177..69266)
                     /locus_tag="ACL8A1_000362"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(69228..69230),aa:Ser,seq:tga)
     gene            complement(69277..69350)
                     /locus_tag="ACL8A1_000363"
     tRNA            complement(69277..69350)
                     /locus_tag="ACL8A1_000363"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(69314..69316),aa:Ile,seq:cat)
     gene            complement(69364..69437)
                     /locus_tag="ACL8A1_000364"
     tRNA            complement(69364..69437)
                     /locus_tag="ACL8A1_000364"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(69401..69403),aa:Met,seq:cat)
     gene            complement(69443..69516)
                     /locus_tag="ACL8A1_000365"
     tRNA            complement(69443..69516)
                     /locus_tag="ACL8A1_000365"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(69480..69482),aa:Pro,seq:tgg)
     gene            complement(69532..69605)
                     /locus_tag="ACL8A1_000366"
     tRNA            complement(69532..69605)
                     /locus_tag="ACL8A1_000366"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(69569..69571),aa:Arg,seq:acg)
     gene            complement(69615..69700)
                     /locus_tag="ACL8A1_000367"
     tRNA            complement(69615..69700)
                     /locus_tag="ACL8A1_000367"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(69664..69666),aa:Leu,seq:taa)
     gene            complement(69708..69779)
                     /locus_tag="ACL8A1_000368"
     tRNA            complement(69708..69779)
                     /locus_tag="ACL8A1_000368"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(69745..69747),aa:Gly,seq:gcc)
     gene            complement(69802..69874)
                     /locus_tag="ACL8A1_000369"
     tRNA            complement(69802..69874)
                     /locus_tag="ACL8A1_000369"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(69839..69841),aa:Thr,seq:tgt)
     gene            complement(69893..69974)
                     /locus_tag="ACL8A1_000370"
     tRNA            complement(69893..69974)
                     /locus_tag="ACL8A1_000370"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(69938..69940),aa:Leu,seq:tag)
     gene            complement(69980..70052)
                     /locus_tag="ACL8A1_000371"
     tRNA            complement(69980..70052)
                     /locus_tag="ACL8A1_000371"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(70017..70019),aa:Lys,seq:ttt)
     gene            complement(70077..70149)
                     /locus_tag="ACL8A1_000372"
     tRNA            complement(70077..70149)
                     /locus_tag="ACL8A1_000372"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(70114..70116),aa:Asp,seq:gtc)
BASE COUNT        21039 a        15727 c        12827 g        20557 t
ORIGIN      
        1 ttattatttc attttactat actctgttaa tttatatgag tttaaaccga tttcatcttt
       61 aacctcgagt aaagcagttt caaatatttg tttaagagtt tttgattctt tacaattaac
      121 cgacaaactt tctgataaaa tatgtacaac ttctgagact gaataaccta tctcctcttt
      181 agaattatat aaatctgtag ctccaccaat aatccaaaaa tactgatttt gtgaacctac
      241 aatatcctca ttttctacgg aacttccttg tatcgaacaa attttattta tctttaccat
      301 aatacttcaa cccttttagt gtcaaaagta aaccaattcc tgtcactgtt aagaatagtt
      361 ccataatctt gttcgaacca gtctttggta atttttgttt tacatctact atttctttag
      421 atttattaat atgattttca gtttctctgc catctccaac tattttatag tttacttctt
      481 ctgtcttatt atcttgttta ttgtcgatct tgtcattcat ttgtctatta tctttacttg
      541 agttaaactc tccgttcttc tggttactat caattacatt atttgaatta tattgttttt
      601 cctctttgtt tttttctttt tcgtttttat cacttaaatt atttgttaca attttgtaaa
      661 gcccattctc cgttacaata ttgaaattac catcgctatc acgtataaca ggttctttcc
      721 catttgcatt agatttgatg aatgatatat acttaccgga taaattataa aattggttat
      781 ttaaaacggt tattttaccc tttgaatcct caataacaat tccttcttta cccccgaata
      841 tactattctc tgagactact aaattatcac taactcgtaa taattcaata tttggttgtt
      901 ctgtttcctt agaattatta ttaattacat tttttgttac agtaatatct gatacttttc
      961 ctaaatattc tgcactatct tttgcaactc gtatcgcttt tgttttagga tcggcaatat
     1021 taaatatatt ttcggcgata accacctgtt tatttaaatc taataggtct ttagtgtttt
     1081 tatagctata agcatttact aaagctgctc cgctttctcg ataatgtaca ctctctcctt
     1141 taactttctt atcaaagcga tttcctttga ttaatacatc agtgaatcct gtaaaacgta
     1201 cacctgcata catcatgtta tcaaaatgat tatttaaaat tttaatatta gaggggttct
     1261 gtgtcgacaa tgtttgatag tgtgtgccaa ttgctgttac taattcccca gatttatcac
     1321 ttttgccaaa ataggaattt tgaatagtca cattttcaga ttttttccca tcatcattca
     1381 aggcataagg aaaacctttt ctagttaatg gttcaatctg aatgctctcc ttacttatga
     1441 tttgcccatc cttcatcgtt ttgggtaagg cttgcccaag aaaacgagaa ttatcaacta
     1501 atacattttt cgaacctgca atttgaatag catgcccttg ataactatcc ttgaatgtta
     1561 cattttttat agttacgtta tttgaattcc caatagcaaa tgcacctgaa gaatttataa
     1621 gtggtagatt ttttgcttta gttccttctt cattcaaagc accgttcata tcaatcgtac
     1681 caccagaata actaatatct tctgttgggc cccattctac ttgcgcacca tcatccgtaa
     1741 ataaacctgt cataaaaaca atggaagggt gattcttaat atttatacca tttagaattg
     1801 tagctttctc attcaattct aagtgtgtat gactttttaa aaaaacaatt tcttttacta
     1861 aataagttcc ttcaggaaaa tatacatttc ctccacctag cccttgagct gcagcatcta
     1921 ttgcatcttg aattgcttgt cgatcatcat gaatcccatc acctaccgca ccataatctt
     1981 ttacacttaa agcagtatct tcaaattctt ttttgataat tctgtcactt ataacatctt
     2041 ccttataact cttattttca ttagtagatg ttactgtaga ttcaactgtt ctgttgtcta
     2101 attgtgaatt ggaacgaata gtaccattag agctgatact attaacactt aacgaattat
     2161 ctaaaccatt agtggttgaa ttagaggcaa tcatttcagc agaacgattc tgattaacag
     2221 ttggtgaatt atctaaatca ttagtaattg cattagaaga ctcttttgta gccatcggtg
     2281 aagaactagt aacaacttca tccgataaaa taccttggct tcccaaaatt gaaattgcca
     2341 atagtaccga gcaagtcccc acctttaatt tacgaatact gaatacctca ttaggaacaa
     2401 aatatttcat cagaacctcc aattttttta ctatttttaa caattgatta ttacttttct
     2461 taaaatagat agaatgacac cgctgtcatt atctttatca tatattttcc aaattgaatt
     2521 gtcaaggaat aacacaagaa aatattaaaa tttatactct tcggaaatct cttcaaacta
     2581 ggtcagtgcc gccttgacct aagtctaggt tactgacttg gtcagttcta tagtagactg
     2641 aaactaaaat agtacacctc tacttctaaa acattgttag aaatcgattt gactgtcccg
     2701 atcaatttgt cctattctta tttcatttta ctatatctac aacctcaaag cagggttttg
     2761 agctatctat ggccaacttc ctagtttgct ctttagcctt ccattcaaaa gtgatgctat
     2821 ctttcttagc ttgaatataa aatatcccat ttactatatt taaatatatt gatatgttgt
     2881 aaattataaa tgaaagaaca aataccttgt catacaagca ttcgccaggg atatttacct
     2941 taatatcgct catatggcgg attgaaacta aaacactaca atatatttct taaatagacc
     3001 ttctataaaa gctagattta acctcgataa aaatagatgc agtgcttcta aaatactgga
     3061 atagggcata taggtaatcg tagacaagtg ttttatagat tgtctcgcag gagatctaat
     3121 gatgtatagc aagcatcact aaacgtatag aatatttgtg ttattataat atcaatatgc
     3181 tataaattct atcatgcaaa agaatgacta gaacatgaaa gacaggcaca aaactttgaa
     3241 caatattctg aaaacaaaaa aagacgcgag tttccacaat ctcactcatg gaaactcacg
     3301 tctttcaaaa aatagggtga attgaaaact agaacaagac actagatttt ctaaatgatg
     3361 tctagtaagg attctaaatg ctacaccaaa ttgctcacat cttatttcaa accacgggac
     3421 tcccacaaga aaggaaaatc ctataaaaat tctctctctg aggagaaaga ggcctcaact
     3481 acaggtgtaa actattatca attgaaaaca acagttttta cactcaatta gactttttag
     3541 ggacaggatc actttcgctt gactgtagtt tgtcattttt ttgataaagt tagcactttt
     3601 tcagaaactg ttttgaaagt ctcaatgata tagtctactt cttcatcgct taatttagta
     3661 tgaagaggga gggtaatttc attctcaaag aaggcatagg ccttaggata gttcgtcata
     3721 tcaaatccaa gattcttata ggctgtcaag agaggaagcg gtttgtagtg aacattactt
     3781 gcaattcctg ctttagccaa ttcttggatg atgaggttgc gttcttctag gcttgctcct
     3841 tctacacggg tgatgtagag gtggcgtgaa gattcgacag tttcagtctt gtgtgccaaa
     3901 ggatggatgc gagaacctgc aaaaccacta tcatagcggt ccacaatgtc cttacggcgt
     3961 tgcaacaaac ttggatagcg gtccaattgt accaaaccaa gtgaagccat gatatcggtc
     4021 atgttgcact tataggctgg tgtaacgata tcgtattccc atgaccccag ttgcatcttg
     4081 gcaagagcat ccttagtttg cccgtgaagg gaaaggattt ggaattcctt gtacatctct
     4141 tcgtcatcaa tcactggatt ggctttccaa gtcgcacttc caccttctgc cgttgtaaag
     4201 ttcttaacgg catggaatga gaaggaagta aaatcagcga tagaaccaga aggttgtcct
     4261 ttataagtag atcccaaagc gtgggcacta tcagagacaa tgacaatacg gttaaaggcc
     4321 ttttgccact tgcttgaagc ggtaaagaag tcacgttttt tctccacgac ttggaacaaa
     4381 cggtcataat cgcaaacaat ccctgcgaac tctactggga taatcacctt agttttctca
     4441 gtgatagctt gctcaagcag gtcatagtcc atctcaaacg tatctgcttg gatatccacc
     4501 atgacagggg ttgctcccac gtgcgtaatg acactacatg aagccgtata ggtcatggct
     4561 ggaacgatga cttcatcacc aggtcccact tccaaaacgc gtaaaatcaa ctccagagcg
     4621 gctgtcgcag agttgagaca aacagtctta ggtgtctgtg tgtaaagaga caagcggcgc
     4681 tccagttctt ttgttttagg acctgttgtg atccaaccag aacgcagggt atccgctact
     4741 tcagcaattt ctgcttctgt gatatcaggc ggtgaaaatg gaatattgta atttggcatt
     4801 gatttgctcc ttttatctgt actcattttc ttatgactac tttattttag tacctcaaac
     4861 acggtttgaa acatgatttt gatgtcccca aagaaactaa actctcggag ataggcgagg
     4921 ttatagcgca tcttttcagg aagaacatgc tccacatagg cctgatcaac tgacagacct
     4981 ttctccgtca tttgactgat gatggtgtcc tcatccttgt agttgatgct ggctggagag
     5041 gtaattcctg cttgcaagag caaggttgcc atcatttcag ggctatactg ctctgtataa
     5101 cgtggcactt caggtcgtgt accgacaaag gacatctcac ctttaaggac attgaccaac
     5161 tgaggcagtt cgtccaaacg gacacgtcgg atgaaatttc caaccttggt aatacggcta
     5221 tcgttagcag aagtcaccag acttcctttt ttatccgcat ccgtcaccat ggtacggaac
     5281 ttccaaatct tgaaccgacg gttgtactgg gtcacacgct cttgcttgta aatcactggc
     5341 cccttactat ccaacttgat ccaaatgctc aagatgagaa agatgggaga ggttagaacc
     5401 agtaagacca aggccaaaaa ccagtccaag caacgcttga aaatcagcga acccttcctt
     5461 ttagagacaa gctggtagta agactcaacc tcgcttgatt tcatttccat aggcaagtct
     5521 tcccatttca gcatgctgtt ctcctttgtt tttattatac tatttgtcaa catttttcat
     5581 tataccacaa aatggaaaag gcggtgaaag aaatctgtaa tttgaaggat ttccttctag
     5641 tactcaatga aaatcaaagt acagctttga gattgcagat aaagctgaca tggtttaaag
     5701 agattttcaa agagtatagg tcatggcccc tgcctgcaaa ctatacatct tgtgataggt
     5761 tcctcccaga gccaagagtt cctcatgggt tccactctcg ataatgcgtc ccttatccaa
     5821 gacatagatg cagttggcgt cttgaatagt agaaaggcgg tgagcgatag caatagttgt
     5881 tcggccctgt ctcatcttcg ccagagaagc ttgaaccaag ctttctgttt cagagtcaat
     5941 attggctgtc gcttcatcca aaatcaggat tttaggctgg ctggcgactg ttctagcaaa
     6001 ggcaagaagc tggcgctgcc cagtagagaa gctcgaacca cgctcggaaa caggggagtc
     6061 gtacccctga ggaagttctt gaataaagga atctgcatcc acaaaggctg ccgcagcctg
     6121 aacctgctca tcactgattt cttggtacat ggcgatattg gacttaatag ttccatgata
     6181 gaggaagggt tcctgtaaga ccaaaccgat gttttttctc agctcttctt gactgaaatc
     6241 cctgatatcc acatcatcca agagaactct ccctgactgg aattcataaa agcgcatgag
     6301 gacattgata atagacgatt tccctgaacc tgtatgacct acaaaggcaa tggtttcacc
     6361 cttattaaca gagaaagaaa tgtcatccag aatcggatgt ttaccgtcat acgagaaaca
     6421 cacatgttca aaacggatat tgccttcttt gactttggct tgcccatttt cttgaagggg
     6481 ctcataggtc ctctcatcga ttaaggcaaa gacacggcct gcagaaacca tagacgtttg
     6541 cagtgttgaa aagttttgcg tcacctcaat caaagggtca aagaggcggt tgatgtactg
     6601 gataaaagca tacatggtcc cggccgttat cccgatagaa agaccacgat agccaaaata
     6661 ggtcatcaag acagcataac ctagaagttt caacaaactc atggcaggtc tcaaaaagag
     6721 ggcatccaag gctacagaac ggttggcata ggccaagtgc tcttggttga tttcatcaaa
     6781 ttctgcctgc aggcgcttct cttgattaaa agcctgaata atcctgattc cctcgatatt
     6841 ctctgccagc ttactattga tatctgacaa gagacttctg gttttctcga tgattttcac
     6901 tgactttttt cgatagagat tgaccaaaag gaaaatcaag gggagaaaga gcaagactaa
     6961 tgctgtcaaa cgaaaatcca gcaccaacat ggtataaagg gttgtcagaa agataaaaac
     7021 tgctgagata aagctggata aaatcccaga aaacatatca ctaatcgtct cggtatcgtt
     7081 ggtcaaacga gaaacgatag aacctgctgg cgtcttgtca aagtaagaca tgcccagttt
     7141 ctccatattg gcaaaggcat cccgacgaat atccctaaca atactgtaag acacgcgcgc
     7201 aaagagaaga ttgccgacat actgaactac agtttgtagg atgtagagac catagtagac
     7261 cagcaaaacg gtcacggcta gttggttaag attgctgaga tactggtcga taaagtggga
     7321 agccacaagg ggaatgacac ttttaatgac cgtcgtcgct aggagaaaac tgagtgccaa
     7381 aaaggtaagg agtccataag gcttgagata ggacatcaag cgcttcaata cagtccattg
     7441 ttcttgttta ttctgcatct tcttctccct tcatttccag ctgctgagac tggtaggttt
     7501 gggcatacca gccatccaaa gctagcaagt cttcgtgcct gcctcgttcg ataatttgac
     7561 cattttgcag gactaaaatc aaatctgcat ggacaactgc actcaggcga tgggcagtga
     7621 taatggttgt cttgtccttt cgcgtctcct tgaggttgtc gataatcgca tactctgtct
     7681 tggcatctac ggcggataag gaatcatcca aaatcaagat atcagggtct aaaatcatag
     7741 cccgactcat agccaaccgt tgcttttgac caccagaaag actgactcct ttttcaccaa
     7801 tcagcgtatc aaatccttga ggcatgtcta caatatcttg gtaaactcgg gctagcttag
     7861 tagcttcctc gaccgctgaa aggggcaagt taggattgcc aaagcggata ttgtctagga
     7921 ttgaagtcgc aaaaagaaac tggtcctgag gaacatagcc catgagactg cgaaggtctg
     7981 tcagacgata gtcccgaata tcgtgaccgt ttagataaat ggcgccctta tccacatcgt
     8041 attcacgcaa gaggagcttg attaaggacg ttttcccaga gcctgtctgc ccaaccaagc
     8101 ccagtgtttg cccttttgcc aaactaaagt gaatatccgt cagtgtttcc tcattttcaa
     8161 aagcaaagct gtcaatggca tactccaaac gcccattttc aataccatcc agaggaaact
     8221 ccgggtcttg tacaggagat tcctgagaca aaagattttc aatccgctgg taagaaacct
     8281 tccctcgctg agtagtatta aagaggaagc cgatggccat aagaggccag accagcatat
     8341 ccaaatagct gataaaggtg actagattcc caactgtaat ctgcccttcc tgaaccacca
     8401 aggagccaac caaaagcgtt aaaacatagg acgaaccaac aaacaagaga accatagggt
     8461 caaagagact atcatatttc atggtttgca ggttcttttg gaaggttaat tcattgactg
     8521 cctgaaaaga cttcaactcg tctgcctgat aaccgaaaga cttggtcact ttgatacctg
     8581 atacggactc ctgtaccttg ttattgagtt cagaaaaagc agcttgggat tcgccaaagg
     8641 ccttatgagt ctttctccct aggcgactag tcgcataggc catgaaaggt agggggagaa
     8701 tggcaacaag agtcatttgc catgagatgc taaagagcat ggtcaacaaa gtcaccagag
     8761 ccgtgataga ggcatccacc gcagacatga caccgccacc tgctaaacga gtcaaggcat
     8821 tgatatcatt ggttgcgtgt gccatcagat cacccgtccg ataggtttga taaaaggctg
     8881 acgacatttt tgtgaaatgc ttaaacaagc gagaccgcat gatctgtccc aagcaataag
     8941 aggtcccaag gatatacata cgccacacat agcgcaaata gtacatacca aaggctgcaa
     9001 gtagcaagta aaataggcta agaaggaggt cctgctgggt taattgcccc gatgtgatgg
     9061 catcaatgac ccgccccata accataggag gaatgagatt gaggacggaa accaagatca
     9121 gggccacaat tccgactaga taacggcgtt tttctaactt gaaaaaccac caaagttttt
     9181 gaataatgga cataaaatcc ctttctgatt gcaaatggaa acctgaggcc aatactcaat
     9241 gaaaatcaaa gagcaaacta ggaaactagc cgcaggctgc tcaaagcact gctttgaggt
     9301 tgtagataga actgacgaag tcagtaacct acatacggca aggcgacgtt gacgcggttt
     9361 gaagagattt tcgaagagta caagacccca ggtttttctt attcataagt tacgactgta
     9421 acagcaccct tgtcatattc agcaataaag atattggcta cattgtcatg cccttgttta
     9481 ctgaggttat caagcaacca ctcttcgcta cgaccaatcg attccaagac atctacttga
     9541 atcacaccgt cagtcacaac tggatactta ggattttcat ctcccatttg cacaacgatg
     9601 agttgcccat tttgctcttg cacagctcgt ttgacttgct tcatctggaa aatcccttgg
     9661 ctacgaagtt tgagggatac atccgatgca gacaaaccaa ctgaacgaca ggcttctggg
     9721 tcaatattcc catttttgat aaggagagtt ggcttaccat caatcaaacg tttcacaaag
     9781 tgaacattgt tgttgagcta tttgagagtc aagaccagtt tgattaaaat ttcgatataa
     9841 ttaagtgtca tttgttcaca tccaccagtt caattgcgtc tgtcttatac aattcgactt
     9901 tctctaacag gtacttgtct ggctcacttc cgtttagggc acgataatag cgatctccca
     9961 ccttgataag tgcgccatct gtgtttgtgg aagtattaac atagacttct gacttgtcta
    10021 ctcacaaatc tttggaaaca acctcgatga aatgaagtga agtttgaaat ttattgttag
    10081 aaacttgatt gttttgataa gtcgtgatgc tgaccaaaag cagagctaaa taaggccaga
    10141 tctgaaatca tgaccaactc acgaaattcg cttccatttt tattatggta tgccttgaaa
    10201 gcaaaaaaaa ctgtcacagc taatagaaca atcccaaagc caaccatgat accattttgc
    10261 tgaccgattt ggctgagtac atagtcataa gagtagaatt tcatgtattt tcactccctt
    10321 tcataaaatc attcttaatt ataaacgaaa gagagtaatt tttcaaacca tacgttgggg
    10381 aaatagatct ttttttggta taataaaatc tataatctga atgaaaaagg taactttatg
    10441 aaacttatct catggaatat tgattcccta aacgctgccc taactagtga ctcagctcgt
    10501 gccaaattgt cccaagaagt cctacaaacc ttggtcgctg aaaatgctga tatcattgct
    10561 atccaagaaa ccaagctttc tgccaaagga cctacaaaga aacacgtgga aattttagaa
    10621 gaactcttcc caggctacga aaacacgtgg cgctcttccc aagagcctgc ccgtaaaggc
    10681 tatgctggaa ccatgttcct ttataagaaa gaacttacac ctactatcag cttcccagaa
    10741 atcggtgccc cttctaccat ggacttggaa ggtcgtatca tcactctaga atttgatgca
    10801 tttttcgtaa cccaagttta cactccaaac gctggtgacg gtctcaaacg cttggaagaa
    10861 cgccaagtct gggatgccaa atatgctgag tatttggctg aactagacaa agaaaaacca
    10921 gtccttgcga ccggtgacta caacgtagcc cacaatgaaa tcgaccttgc aaatcctgct
    10981 agcaaccgcc gttcacctgg atttactgac gaggaacgtg ctggatttac caaccttttg
    11041 gcaactggat ttacagacac cttccgccat gttcatggcg atgttcctga acgctacact
    11101 tggtgggcac aacgcagcaa aacttctaaa atcaacaata caggctggag aatcgactac
    11161 tggctcacaa gtaaccgtat cgctgacaag gtcactaaat ctgatatgat tgactcaggt
    11221 gctcgccaag accatacacc gattgtcttg gaaattgact tgtaaggatt ttctatatgg
    11281 actacaatgc ggtcattccc gagttgcttg tatcgaatat cgaacagtcc cggtccttct
    11341 actgtggttt actgggtttt aggattgaat actaacgtcc agaagaaaac tttctctttc
    11401 tcttgaagag tgtcaactaa tgttagagga gggaacaaag gatcaactag ctgaactgac
    11461 ctaccccttt ggtcgtggag tcaatctctc ctttggcata aaggatgttc caaaactcta
    11521 tcaaaaagtg atggaggcaa actatcctat ttatcgtctc ttgaccaaaa gaaagtttcg
    11581 tgtcagtgat ccctatatct atcctcataa atttgcagtt ttggatccag atggctattt
    11641 tttaagattt agcgagtaga ataataaaaa ttgggaaaag tctttaaagt tagaaaaagg
    11701 catattgtca ggtgtctaaa aacttgatag tatgcctttt atgatggaaa aatcttctaa
    11761 tactcttcga aaatctcttc aagccacgtc aacgtcgcct taccgtaggt atatgatact
    11821 gactctgtca attctatcca caacctcaaa acagtgtttt gaggttgtgg atagcttcct
    11881 agtttgctct ttgattttca tttagtataa atgttagctt acaatggagt ttttgttcac
    11941 cctctcttct gtttgttcgt tttttaaacc aaaacactaa caaaatagtt gatttctaaa
    12001 gttatttagt gtaaaataaa aacattggct caagcctatt taaaattgaa tatcgtttta
    12061 cttgtttatg tcgtgaattg gcacgacgtt tctacaaggt gccggaacac ctaacaataa
    12121 gtaagtcagc agtgagatat ggccgttttt gccatgtcta tttaggggac ttacttagag
    12181 attaagtagg tcttttttga atatttttaa tatttccctt aattagtgcg gacgggaggt
    12241 gaaattatcc tgtgaataat ttcaggaatc cagggaattt catccctaat ttttgagccc
    12301 ctagctcttt catttctagg cttaggctaa aatagttccc cgaactattt tactctcaaa
    12361 aatcccgtca tagacaataa caagattatt gtctatgata ccactacaac gggaaatatt
    12421 tgataagact cgcttcgctc gtaatagttt tatcaaatat atagtattag caaaagggat
    12481 cactatagaa acatttaaac aacaagtaaa ttaactttta gactttagga ggtcttatga
    12541 aattattaga agagcgcatc ctcaaggatg ggcatatctt gggtgataac atcctcaagg
    12601 tagattcctt tttaacccac caagttgact ttagcttgat gcaagagatt ggtaaggttt
    12661 ttgcggaaaa atttgctgct actggcatta ccaaggtcgt aaccattgaa gcgtcgggta
    12721 ttgccccagc cgtttttaca gctgaagcct taaacgttcc catgattttc gccaaaaaag
    12781 ctaagaacat caccatgaac gaaggcatct taactgctca agtctactcc tttaccaagc
    12841 aggtgaccag cactgtttct atcgctggaa aattcctctc accagaggac aaggttttga
    12901 ttatcgacga tttccttgct aatggccaag ctgctaaagg cttgattcaa atcatcgaac
    12961 aggccggtgc cacagtccaa gctatcggta tcgtgattga gaaatccttc caagatggtc
    13021 gtgatttgct tgaaaaagca ggctaccctg tcctatcact tgctcgtttg gatcgttttg
    13081 aaaatggtca agtcgtattt aaggaggcag atctctaatg caaactcaag aaaaacactc
    13141 gcaagcagcc gttcttggct tgcagcactt actagccatg tactcaggat ctatcctggt
    13201 tcccatcatg attgcgacag cccttggcta ttcagctgag cagttgacct acctgatttc
    13261 tacagatatc ttcatgtgtg gggtggcaac cttccttcaa ctccaactca acaaatactt
    13321 tgggattgga ctcccagtcg ttcttggagt tgcattccag tcggtcgctc ccttgattat
    13381 gattgggcaa agccatggta gtggcgctat gtttggtgcc cttatcgcat ctgggattta
    13441 cgtggttctt gtttcaggca tcttctcaaa agtagccaat cttttcccat ctatcgtaac
    13501 aggatctgtt attaccacga ttggtttaac cttgatccct gtcgctattg gaaatatggg
    13561 aaataacgtt ccagagccaa ctggtcaaag tctcttgctt tcagctatta ctgttctgat
    13621 tatcctcttg atcaacatct ttaccaaagg atttatcaag tctatctcta ttttgattgg
    13681 tctggttgtt ggaactgcca ttgctgctac tatgggcttg gtggacttct ctcctgttgc
    13741 ggtagctcca cttgtccatg tcccaactcc actctacttt gggatgccaa cctttgaaat
    13801 ctcatctatt gtcatgatgt gtatcatcgc aacggtgtct atggttgagt caactggtgt
    13861 ttatctagcc ttgtctgata tcacaaaaga tccaatcgac agcacgcgcc ttcgcaacgg
    13921 ttaccgcgca gaaggtttgg ccgtacttct cggaggaatc tttaacacct tcccttacac
    13981 cggattttca caaaacgttg gtttggttaa attgtcaggc atcaaaaaac gcctgccaat
    14041 ctactacgca gctggtttcc tggttctcct tggactgctt cctaagtttg gcgcccttgc
    14101 ccaaatcatt ccaagctccg tcctcggtgg tgccatgctg gtgatgtttg gttttgtatc
    14161 tattcaaggg atgcaaatcc tagcccgagt tgactttgct aacaatgaac acaacttcct
    14221 tatcgcagct gtttcaatcg ctgcaggtgt cggtctcaac aacagtaatc tctttgtcag
    14281 catgccgaca gccttccaaa tgttcttctc aaacggaatc gtcgtagcca gcctattcgc
    14341 tattgtcctc aatgccgtat taaatcataa aaagaaataa gaaaaagagg tgcgagcctc
    14401 ttttttgttt attacaaatt gattataaat acaatcaatc ccaattccca tctaattgaa
    14461 ttggacctat ttcaaattct ttaaaatatt atgttccaaa tcgttaatat tatataagaa
    14521 tggtaaaata tcaaatgttt ctcgtaaatt attcttagcc ttataccaac cttgtccatc
    14581 agtaatccac ataaactcaa cattaggaat agctttaatt tcttcagcaa tcattttgta
    14641 agatctagcc gtctcattta attttgaacc acttcctgaa taaaaattaa cctcaatcag
    14701 ataaagcact tgttcatttt ttatgacaaa atcaaaacgt ttcacagtat tcccatcatt
    14761 cgtaatagca gataaatcga cagaaaaaat ttcctctatt tcattttgtt caatctcttt
    14821 aaataaattc tctcctaaaa tatacccttc agcttcaaga tagctttgaa caatattttc
    14881 catcgcatct ccagtccgat ttttgcgccc attagtatcc ataccaacct caacacctgt
    14941 aacgtagtca actaaatttg atactaaatg attttgcaat aaatcaaata tacctgattt
    15001 ttccaaaaac atagtatatt cttcaatact atagtttcgt ttactaaaat caaaatagaa
    15061 atcttttatc ggatccttaa caataatcgt atctctcaaa cgtttagcaa taagtagagg
    15121 cacaactttc aaaatttctg gataattttg atataagtct aaaaaatcct cttgtatatt
    15181 tttactacca ataagactat tcataatatt tagagcaact tttatactag atacattttt
    15241 ataaacctta ggaaaatctg tataataagt ccagtcagct actgtgtcgg tcatcgttga
    15301 caaccactca tcaaaatttc tagtttgttt cattttctgc ctccaacctc ttccttgcaa
    15361 tttttaaata ttctttttca gcatcgatac ctataaatct tctacctaac cgcttcgcaa
    15421 caacacccgt agtgccacta ccaacaaatg ggtctagaat atagtcaccc tctttagtag
    15481 aggctaaaat aatgcgttct aacaaatact ctggtttttg agttggatgt ttcccagccc
    15541 atttttcaac tttctttgtt aaagaaccgg tccagacatc tttcatttgt tttccatcat
    15601 tcaattcttt cattaaatca taattgtagt aatgacgagc ttttttatca ttttttctgg
    15661 cccataaaat ggtttcagta gaatgggtaa aataacgaca agataaattg ggggcagggt
    15721 ttgttttctg ccaagtaata ttattcagaa ttttaaaacc ttcttgttct aatgccattc
    15781 caactgagta tatgttgtgc aaactacctg aaatccatac cgtcccatta ggcttcagaa
    15841 cttcttttgc taggcgaatc catttacgat taaactcatg tttttcttcg aatgaagaaa
    15901 ttttatccca atctccttta tcaacagaaa caacttgtcc cccagaatta gatattccac
    15961 cattacttaa aaaataaggt ggatcagcaa aaatcatatc catactttct ggtttcattt
    16021 ttgataagaa cttgaacgta tctgaatgaa caagaatcat cttattttta ttgtagtatg
    16081 gttttgtcat aagaacacct ccatacttat attcgttatt tttcataatt tgtgactata
    16141 atttcagaaa tttttcctcg acttgaagat tttgctccat tagttcgggt agcttcaaca
    16201 taatgtatat taaaatcctt atacaactcc tctactaaag cactagaaga atttgataac
    16261 ataacataag ctcctgtatc actcaatctc ttaaaggcat ctcttaatct tacttgatct
    16321 gcaaaagaga atccctcatg agtataagac gtaaatgcac tcgtctcaga caatggaata
    16381 tatggagggt caaaatacac aaaatctcct gttcgaacat ctacaattgc cttttcaaaa
    16441 tctcccactt taatttctag ttgattgtta tttatataaa ctgaaatagc agatatcaat
    16501 tcctcatcaa caattttagg attcttataa cgtccatatg gaacattaaa ttgattctta
    16561 gaattcacac gatatagacc attaaagttc actctcaaca tatatagaat acgtgcagct
    16621 ctttgtactt cggacatcat atctattctt tcatcacgat ctgcagaacg taaatctaaa
    16681 taatattctt ttgaattata ttcctgatga actttcaaaa tttcaatcaa ttcttgagga
    16741 ttgtccttaa tttgttgata gcaatttatt agttcagcgt taaaatcatt aataactgca
    16801 tctttaggag ccaaatcaaa aaataaagct ccacctccaa caaaaggttc gaaatacctg
    16861 ttataggttt taggcattaa ttctctaata acaggcaata attgtctttt accacctgtc
    16921 catttcgtga acggttgtaa agtaactttc tttatttctt ttatcttcat gcttttaatt
    16981 ataccataga aattaaaaca caactcttgt attttctacc acattactaa attttctatt
    17041 ttagaattaa atcatatcaa tatcaaaaaa tgagtaaggc aaatcttata aattccaaaa
    17101 ttcacctcac tctattaaat tactctttat atccttacct gtctttcttc tgaccgctgg
    17161 cctgtgacaa acatggtaaa ggttgcttta cagacatttc tgccctcttg attggtgata
    17221 tccacatcca tcacacaggt tgtacggcct tgatggacac attctccttt aatggtcaat
    17281 acatcgtcga gttttcctgc cttaaggtag ttgatagagg attggagtgt caccccatca
    17341 agtcccagcg agataaccac caaaccactg atttggtcac atagcgtgaa tagatagcca
    17401 ccatgggcat tgccatagta gttgagcgac gagttcacta ctttggtcgt caccacaacg
    17461 tgaccatctc tcattttttc aatttcgtaa ttttcaaagg cagatatagc gtcaaaatga
    17521 aagtctttca tcgtttcctc ctgatatttc aaacgacact atgatactac tttttatagg
    17581 actgtgcaag gagaaagctc ctagtctagt gaaattccga catgttccac aaagcgcata
    17641 aaggctgtct gcccttgttc tgtctgcttg tagactcctg catcctcaag tacacgcaca
    17701 aagatagcac ccacagagtc cttgacgatt gcaagggctt tttctttatc cgttaggtct
    17761 ggatgttggg atttgagttg gtctgcccac tcctgatgat aatcggcaac tgtaacagct
    17821 tctcctacaa gatagctagc gacttgctcc acttcttctt tcagacgtgg tggcaagatt
    17881 gccaagccca tgacctcaat caagccgata ttttccttct tgatatgttg gacatccttg
    17941 tggggatgat agatgccatc aggatgctct gctgaagtct gattgtctcg caagaccaag
    18001 tccaactcaa actgtccatc acgcttacgc gctattggtg tgatggtgtg atgcggtgtc
    18061 ccgtctgtct ctgccaaaat ctgcacacta gaatctgaat actgacgcca ttcctggaaa
    18121 atcttatcag ctaagttaat caaatcctct ttggaatccg aagtcaagcg taggacagac
    18181 attggccact tgataattcc agccttgacc tgctcaaaac cagcaaatcg gaaggtcttt
    18241 tgcaagggag ccaattccat aggaaatacg tgacggcctc cctgataatg atcatgagtt
    18301 agaatagagc cccccacaat cggcaggtcg gcattagatc cagcaaaata tcctggaaac
    18361 tgctctacga tagccaacag acgttcaaaa ctctgacgac taatggccat gggacgatgc
    18421 tggccatcta aaaagataca atgctcatta aagtaagcat agggcgaata ctggaacccc
    18481 cattcctgac caaccatttc aaaacggata atccggtggt tgctgcgagc tgggtggttg
    18541 acccgaccat ggtaaccctc attctctaaa caaagttgac actgaggata attactagct
    18601 tgcaccaact tggctaccgc aatctctttc ggatcctttt caggcttaga gagattgatg
    18661 gtaatttcaa gttctccata atcagatgga atacgataag cgatattttt agcaatggcc
    18721 ttgagtttga tgtagtcatt tttccgactg agttgataaa aatcctctat cgcttgctca
    18781 ggagagtagg cataggcttc ccagaagtca cgattgacct gactcggaca aggagtcacc
    18841 aagtccatca gttcagtacc aaggatttca cgcgcagtct gactatcctc aatcgtctct
    18901 aatcgaacgg cttcctcaac tagctggtcc ttgaggtcaa tcaatttatc cagattggtc
    18961 tcaacttcca aaacaccttc tcccactcgt gccaaaacac gattggtcag gtagattcga
    19021 tccatttcct caaatgaact ttcagaaatg acatgtgtta caaatttatc tactaaggtc
    19081 actatagagc ctccttttga ctagtcaagg acgcgagtgc cacctgcaac ttcagcgata
    19141 tagaagcttg gagcgtatcc aactacttcc tcgtagtgtt tgcctacagc ttccttaaag
    19201 gcctcaacag tatctttttg aaccaaggca atggcacagc caccaaaacc agcccctgtc
    19261 atacgagcac cgagaactcc ttcttgtgcc caagctgtgt gaacaagagt atccaattcc
    19321 aaaccagtta cttcataatc atgctccaga gaaacgtgtg acgcattcat caaacgacca
    19381 aatgtttcca aatcacctgc ttgaagggct gcttgagctt taagggtacg ttggttttca
    19441 agcacagcat ggcgagcacg tttcaaacga ttttcatctt taatcagata gctatattgg
    19501 tcaacggccc actcgtccaa ttcacccagg gtctgaatat ccaaggcaac ttgcaattct
    19561 tccactgctt tttcacactc agcacgacgt tcattgtatt tagagtccgc caattcacgg
    19621 cgtttgttgg tgttcatgat aacaacgaca ttgtccttca aatcaagtgg caccaagtcg
    19681 tattctaaag tattggtatc taagtaaata gcacgttggt cagcccccat accaatagca
    19741 aattggtcca taatgccaga atttactccg ataaagttat tttctgtttg ttttccgatt
    19801 ttaaccaaat cgagacgctc taattttaaa tcaaagagat gctctgccac tacccctgtc
    19861 aagagttcaa gggatgctga agaagacaaa ccagcaccat ttgggatatt cccataaaca
    19921 taaaaatcaa aacctttgtc aatcacgtgc ccagcttctt gcaagaaatg aaggacacct
    19981 tttggataat tggtccagtt gtgctctttt tcaaacttga ggtcagcgag aggcacttcg
    20041 ataatgccct tgtcctcaaa gttagctgag tagaaacgca agacttggtc atcacgcttg
    20101 cgagctgcac cataagttcc taaggaaata gcagctggaa aaacgtgccc accgttgtag
    20161 tctgtgtgtt caccaatcaa attaatacgg cctggtgaaa agaaagtttg gtctgcttct
    20221 tgaccaaaaa cagcaagaaa gtctttgcga agagtttcag cagtaagatg ttgtgtcata
    20281 tgatttctcc tttgactgtc cgttaagcca ccttaacgtg taatcgtttt cttctattgt
    20341 atccaaggga tttcccaagg tcaatccttt ttactaaaat tttactaaac tatcagtaaa
    20401 aagcagaaaa atatgatata ctaacctaat caaggaggat ttatggctac cttaaaagac
    20461 attgcacagc tagcctctgt ctctatcgcg accgtatccc gcgtcctcaa ccgcgaccag
    20521 agcctatctg ttacagaaga aaccagacac cgtattttaa ccgttgctga agagctgggc
    20581 tacaccaagc acctcaagac aggcgagtcc cataaaccca agcaaaagat tgccattatt
    20641 caatgggtca gcgaacaagg ggagctggac gacctctact actaccagat tcgcctagga
    20701 atagaaaaaa gagcccaaga gttggactac gatatcttgc gctattttaa tgaccaccct
    20761 tttaccctaa gcgaggaagt gattgggatt ctctgcatcg gaaagtttag tcgagctcag
    20821 atttctgcct ttgaagaata ccaaaagcct cttgtatttc tagacagcga tacactttcc
    20881 ctgggacata cctgtattat tacggacttt tacactgcca tgaaacaggt tgtcgattat
    20941 ttcctcagtc aagggatgga ccgtatcggg attctaacag gccttgaaga aacaactgac
    21001 caagaagaaa tcattcagga caagcgtcta gaaaacttca aaaactacag tcaagcgagg
    21061 ggaatctatc atgatgaact ggtctttcaa ggaagattta ctgcccagtc tggctatgac
    21121 ttaatgaagg aggccattca gagcttggga gaccaacttc cgccagcatt tttcgcagcc
    21181 agcgatagtt tagctatcgg tgccctccgt gccctccaag aagctggaat caacctgcca
    21241 gatcgcgtca gcctcatttc ctataacgac actagtctga ccaaacaggt ctatcctccc
    21301 ctctctagta ttacagttta tactgaagaa atgggccgag caggtatgga tattcttaac
    21361 aaggaagtcc tccacggtcg gaaaatccct agcctgacca tgctgggaac cagactgaca
    21421 ttaagagaaa gtaccctaaa tcaagaatag gataacataa aaaacgaata ggagttttag
    21481 aactcctatt cgttttttat tcgattacaa tcatagactt aatggtctta cgttcatcca
    21541 tatctttgta ggcttggtcg atatcttcca gtttataact tgaagtaaag acgcgacctg
    21601 gattgatatc accatcaagg acggctttta gtaaaaattg cttatcgtat gttgtagcag
    21661 aagctgcccc acctgctaca aagatatttt gcataaatgt cgaaccaaga gcacgattat
    21721 tatagtgtgg gactcctaca aagcccatac gccctccatt atgaagaaca cctagcgcct
    21781 gttctatagc aacctccgta ccaacacatt caagtgctgc atctgctcct ccaccgagga
    21841 tttcacgcac cttggtaatt ccttcttgac cacgttctgc aacaacagct gtcgcacctg
    21901 actccatagc catcttttga cggtcttcat gacggctcat aaggataatt tgtgatgctc
    21961 cacgcatctt agccgcgatg acagcacatt gaccaacagc cccatcaccg ataacaacaa
    22021 ccttgtcccc tttttgaaca tttgcaacac gcgccgcatg atagcctgtc ggcatgacat
    22081 ctgcaagagt caaaagggac ttgagcatcc cttctgtata gtcagaaggt tgaccaggga
    22141 ttttaaccag cgcccagttt gcatagtgga agcgaatata ttctgcctga aaatcacccc
    22201 ccaaattatt gccaatatga ttgtcgcaag aaccgtcaaa tccagcaaga caggcatcac
    22261 actcaccaca tccatgtgta aaagggacaa tcacaaaatc acctggtttc accgtcgtaa
    22321 tggcttcccc agcttcttca acaatcccaa tcgcttcgtg tccactattt ttgtgtccag
    22381 ctttcgtttc tggattacgg tacctccata aatctgaacc acaaacgcac gcacgaacca
    22441 cacgaataat cacatcatcc gcttctatta tttgcggacg ttcaatgcta gcaagtccaa
    22501 cctgacctgc ctttgtatat actgctgatt tcatttaaaa ttttccttcc ttataaagtt
    22561 taattttgag atttaaacga tttaaagctg tctgtagctg agacaagcgc tcctctaatt
    22621 tttgcttttc ctcttctaaa ataccaagcc tctcctctct cgtttcatct cccttttggt
    22681 agagcgacat atagtcaact aaactatcta cagagacaca cgccgaacga aaacacttaa
    22741 taaattccag cgcttcgata tcctgatctt gaaaatcacg aatcccagta gcagtacgag
    22801 taatcggtgg cacaagacca acccgttcat aataccgaat cgtatccgct gaaattccca
    22861 acaaatcact ggcagattta atattcacac gctaacctcc tctcatttca ttcactgaat
    22921 tcacgctatt ctaactcact tatcttatta tataactttg agttgactcc aagtcaagca
    22981 ttttgtatct aatcgaaaaa tttttatcat taacaatcat atatctgaag tttatacaat
    23041 gctcttctct taccaatttt tgaaatattt aaataataaa gaaattactc tattccaaat
    23101 aagatagtac ccaagtattt ttcacttttt tctaatgttg atgctcataa ctccgttcca
    23161 agtcacacac ctttcgctta tgggtttggt gagtttctag gtcagcatca atttcaatgg
    23221 taatattttg aaaaccacaa tcttttagga aaattcgaat agactcttta caagtttcca
    23281 tatattccat ttcttttaga caaacatgga caatggcatt tttttccaaa gcatccatag
    23341 tccagagatt aagctgatta aggctggcca cattgtccaa tcgctccagg ccactcttta
    23401 cttgcttgat atcaagacct tctggcacag catccaagaa aatcttgagt gtagaccaaa
    23461 aacgtggaag ggcttttgaa agaataaaga aagaaatgac aagggacaaa agaggatcta
    23521 ggatatacca gtccgtaaat cgaagaacaa tcgccatcag gataacagct acccacccta
    23581 gcgtatcttc cagaaaatgc agactcagaa tagactcatt ctttgtcttt cccttaccaa
    23641 ccaccagact cgctaacaga ttgatagtaa tcgcaataat tcctaaccag agaatcccct
    23701 catcattgac tggttgcgga tgcaaaatct tcgtgacatt ttccaaaatg actagaacag
    23761 agcccgttac gagaatcaca gctgttacca aggctcctag caggctaaac cgcttatagc
    23821 ccaaggtgta ctgattgtct tcttcacgat tggagattgt ttctagaaaa gctgacattc
    23881 caattgcaat cgcatctccc aagtcatgca cagagtcagc aagaacagcg ctagaaccaa
    23941 atactccacc tgcaataaac tcaacaatgg cataagtcaa atttaagaaa aaagccaccc
    24001 aaacagcata ttttgccttc atatttctca ttcctttgtt ataatagatt tatgaacacc
    24061 ttgttcatta tcattatcca ctaaaactca aagaaaggat agaagcaaaa cgtcagcttt
    24121 attcagttct gaacaatttg ccttaagtgt ccatatgact aacattgacc gccgtatcag
    24181 caaaaccaaa aaagccatct atcaagcttt tatacaactt ttgaatgcta agggctacga
    24241 ggccactact gttcaggata tcattgatct cgcagatgtg ggacgatcca ccttttactg
    24301 tcactatgag agtaaagagc ttcttctgga tcagctctgc cgctacctct tccatcatct
    24361 ttttgaaaga gagcaagcca tttcaaccga agactacctc gcccacctct ttctgcattt
    24421 tcagaaaaac caagaccata tcgccagtct actattctcc aaaaatgact atttcctccg
    24481 tcaactacag agagagcttg agcaccacgt ctatcccatg ctggctgctg atttgaagca
    24541 aactcaccca agtctaccta cttcttacct ccaacatttt gtcgtaacta actttatcga
    24601 aacattgacc tggtggctca aaaaaggtca agatttcaca gaccaggaag ttgtccaatt
    24661 ttatctagac cttctcattc ctaaaaattg aatatagagt aaaacttcag ttgtcttatt
    24721 tctaggttac tgagtttttt atcttttcaa caacaaaaga ggacccgccg atcctctttt
    24781 tcatactata aatccttgat tatcaactat atctgtttta atcgaaatct caaaacagca
    24841 ctttcaaaca tcttttccta gttaagtaaa tcagtattct gcttagctgc cttgctccat
    24901 tgataccaac caactagact gttaatgaga taaattagat atttcccttg aatttgcagg
    24961 ctttctcccc accagagata gattgaaaag acattggtag ccgcccagaa tatccactgt
    25021 tcacggtaaa cagctgtcat gaggatttgc cctaccccat tggttgcatc tgtgattgaa
    25081 tcacgatagg gacgattggc accaatagac tgataaatga agccaaaggc caaccaccaa
    25141 agcacactaa tggaaagata ctttgtccag cccttgccgt ccagtttacg cgcgacaaac
    25201 tcctgctttt ccttcttaaa ctgtgcctga taaatccaaa ctagaagtcc aattggctgc
    25261 atgactgtga agtaaagtgt cgtcagcacc tcaccataaa agcctttctg tagggccaaa
    25321 ataaggtaaa taacagagtt aatcaagcca aaaagataat tacttgctcg accttccgat
    25381 acaaagatta cacagataat ccctgtcaag ctacaaatca tcccaatcca gtcaacaata
    25441 cgatgttcgt aaaccaactc cagccagaga ggaaaacttc ctaaaaccag caaataaatc
    25501 cactgggcaa aactacgatg ggcaaagagg tcatcccaga tagccttcat agttcctgaa
    25561 aatcctaaat cagccatagc cgcaaccata cgacggtaac cacctgacat ttcacctagg
    25621 gttgttttga tattttcaat tttcttttgc aaataagtat gcatcatttc tccttttgtt
    25681 tttaaagagc cgtgtctgga tagactttcg gacgcaacgc tctattagat aatgaactgc
    25741 ctatacacaa gatttctaac cttagtcgac atgagctgaa acctcttatt tgttaagtag
    25801 ttcacaaaat attatacacc tattttatga atagtcaact gtctttacag taaaatttta
    25861 gaaaatcatg aaaattttct ctttctttcc attttaagtg acattcagtc attctcacat
    25921 caaaaaagcc cagacgaaat tgtctgagca ttcttttatc tagtcgttta aggaagttga
    25981 gttcagtatg tttaaagtct ctgtcccatc atttcttcaa caaaccttgt tcttggagaa
    26041 actccttggc tacttgcttt gctgacttgc cttcaacacc gacttggtag ttgagctggc
    26101 tcatctggct ttctgtaatc ttaccagcca atgtattaag aactctttcc aactctgggt
    26161 gtttcttgag aagagcttct ttcatgagtg gagccccttg ataaggtggg aagagttgct
    26221 tgtcatcttc caagacctgt aaatcataac gctccaattc cgcatcagtc gaataggcat
    26281 ccgtgatttg aatatcccct gactgaattg cctgataacg aagagctggc tgcatagtcg
    26341 ccacattgag attgagacca tacattgatt gcaagccctt atttccatct tcacggtcgt
    26401 taaactcaag cgtaaagcct gccttcagct gcccttccac ttttttcaaa tccgaaatgg
    26461 tcttcaagcc atattcttga gcaatctttt tcggaacagc tacagcatag gtattttgat
    26521 aagacatagg tttgagatag gctagatgat cctgcttggc aatgccatca cgcgccactt
    26581 gataaacctg ctctggctca tgactcacct tgggtgatgg ttgaagcaaa ctttcagtca
    26641 ccgtaccggt aaattcagga taaatgtcaa tatcaccttt tttcagagct tcataaagga
    26701 aacttgtcgt cccaaaattc ggtttaacag tcgcagtcat gctggtattt tcttcaatca
    26761 gcaatttata catattagcc aaaatttctg gttctgggcc tattttccca gcaataatca
    26821 agttttcctt ctctttttga accaaaagag ctggactata agacagaccc agtaataaag
    26881 ccaccaaggc aaaaccagag aaaatcgtcc gtaattttgc tttttccatt acttttagta
    26941 ggaagttaaa ggcaatagct agcactgcag aagaaagtgc cccaatcaaa atcagactgg
    27001 cattattacg gtcaattccc aaaaggataa aggaacctag tccccctgcc ccaatcaagg
    27061 tcgccaaggt agccgtaccg ataatcaaga ccgctgccgt ccgaattcca gacatgataa
    27121 caggcatggc aagtggaatt tcaaacttct tgagacgctc ccatctggtc atcccaaagg
    27181 caatcccagc ctcttgcaga ctcggatcaa ttcccttcag cccagtaata gtattttgta
    27241 aaatcggaaa aatcgcataa atcactagag ctgtcaaagc cggcaaggtc ccaattccca
    27301 tcaaagggat aaagagcccc aacaaggcca gagacgggat ggtctggaaa atacctgcaa
    27361 tctgcaagac ccagtcggcc agcttctcat gatagcgaag aaaaacagcc aagggaatcg
    27421 caagcaaaat agctagtaac aaggtcaaaa gtgacaactg caaatgttga gatagagctg
    27481 tcaaccaatc actaaaacga tcctgaaaag ttgcaattaa attagtcatg aacactacct
    27541 ccaaacaagt ctgctacaaa gtctgttgca ggcactttta aaattgtctc gggattcgct
    27601 acctggcgaa tttctccatc ctgcaagaca gcaatacggt ccgccaactt caaggcttca
    27661 tccgtatcat gggttacaaa aatcgttgtc atcccaaact ctttatgcaa ttcttttgtc
    27721 agaacctgca actgttttct cgaaatagca tccaaggccg aaaagggttc atccatgagg
    27781 aaaatcttgg gctgaccaat catagctcgg acaataccga cccgttgctg ttctccacca
    27841 gataattcac taggtaagcg atgcccatac tcagctactg gtaaaccaac cttagccaaa
    27901 agctcttctg ttttcttcgt aatttcttcc ttgctccacc ccttcatttc aggaatgaga
    27961 gcaatatttt ccgcaactgt tagatttgga aaaagagcaa tagcctgtaa aacataacca
    28021 gtagaaagac gaagttcacg ctcatcatag tctttgatgc gcttcccatc catataaata
    28081 tttccatcag ttggttccaa aagacggtta atcatcttga gcatggtcgt cttacctgac
    28141 ccagaaggcc ctactaaaac cataaattcc ccatcctcaa tctgtaagtt gacatctctc
    28201 aagacatcct tttctgtgta gcatagtgct acatttttgt attcaatcat tctttgtcct
    28261 catttaaaac ttccctcgat tggtcaagtc ttctacctta ggcatgactt ccttattatc
    28321 ccaatgctcc acaattttcc cgttctttaa acggaagata tcgtactggg cataagcaac
    28381 gccatcaatc tgagtctgac catagctaac cacatagttt ccttgtccta agagttggaa
    28441 aacaaagtca aaagtgacac tatattcagc cacatagttt ttataagcag cacttccttg
    28501 tccaatatca tgattatgct gaatcaaatc gtctgccaca taatcactcc actgctctag
    28561 ctccccattt tggaaaattt ctgtcaagaa acggcgaacc agctttttat tttctgcttt
    28621 cttgtccaaa tccttgattt caaaatctcc aaaaatttga tctagttggt cattttcagg
    28681 tgttcgatag tagtcaatga catcccaatg ctcaacaata cgaccattct catccgcacg
    28741 gaaagtatcc gtcgtcaccc attgagcttc tccaccattc agatattgat gaacatgaac
    28801 aaagaccaga ttgccatcct caatggtgcg gacaatctta atctgacgct ctggatgacg
    28861 ctcaaagaaa tctgcaaaga aggctgcaaa tccttctttc ccgtcaggaa cacctgtcga
    28921 atgttggata taggtatccc ctacagactg ggcttgagcc tcagcaactc gtccgtcttg
    28981 aatggcatgg atgtataggt tgtgagcatt tttcacttgt tgtgacatat tctaaacctc
    29041 atttcccttc tctttcagat tcgccaaaat tctttcttga aaaccttcaa attggtgaat
    29101 ttcttcctct gaaaatcctt tgtaaaagat agtatccaat ttctgactga cacgatgccc
    29161 cacttctttc tgggacttgc ctaactccgt taaaactaaa tacttcttac gcttgtcttt
    29221 tccacacgga ctaacaatta caagcttttg ttcctctagc ttttttatca tagtcgtcag
    29281 cgtattattc gcaagtccag tcgcaagcgc gatatctgtc gcagttgcgc agccagtttc
    29341 actattccat aaaaccgcta aaatcttgcc ctgttcaccc ctataaagag cctcaggatc
    29401 ttgactcagt aacttttgaa aaatccgccc attcaacaaa cgaatatgat gggctagcaa
    29461 atgaccatct ttcataacac ctccaattta tttcgatatc gaaatgaata aaacaattgt
    29521 aacactcatc gttctaactg tcaactattt cgatttagaa ataatttttg ataattatcc
    29581 acaccaccat actccggctc aactaacttt taacgagagt ttctaaactc cttcgtcctc
    29641 cagtctacaa aagccttcca tccgtactat cctatatttt atgaggggac acatttttcc
    29701 tatcagagca tttattttaa agatagaagt aaatcataat tgcttccatc tgttctttta
    29761 tagtatattg aagttggact agggcactgt atcttctaaa acattgatag aaagcgattt
    29821 gaatttccca atcaatttgc tcgtatttat agcattttga aactagaata ggacgccatg
    29881 actgctaaaa gatttctata aattcatttg attttcctaa tcaatttgtt catgtcttat
    29941 ttcatctcgc tataatttca ccttacccta tctttttcgt agcacccttc aaacagccta
    30001 tcccctaccg tttgacgatt cctcacttca ctccacttcc attacagaag tttcttcact
    30061 actatggtct cggctgactt ctcatgattc cttgttacta ctatttgaac gctcacgaga
    30121 taggtcttat aaaaaatgct ttgatccaca atggaatcaa agcattttaa agagttcctc
    30181 atacataagc gcagaagtcg cagttcctct gtacttggct tcttctcttt tgataaagct
    30241 agccaagttg agcaactcag gtgctggatg tttgggattt aggagcaatt cacgattgac
    30301 caggcctgag agacgaactg cctgcaattg ctcatttgta gtaggcagtt ttttagtagt
    30361 ctctaggaga gcagcaacta aatcttcact caaatcatgt cgagcatgat tgtaaagatc
    30421 ttttataagg ctttctaggt ttggttctac catccctacc acctccctta tggtttaata
    30481 atgtttaatc aaatcaaccg ttgaacgatc caatttcttc accaaggctt gtaagaaagc
    30541 ttgcgcttct aggaagtcat ccattgcata gagggtttgg tgagaatgga tataacgagc
    30601 gcagacaccg atagttgttg atgggacacc accatttttc agatgagctg cacctgcatc
    30661 tgttccgcct ttaccacagt agtattggta cttgatacca gcttcttcag ccgttgtcaa
    30721 aaggaaatcc ttcatccctg gaagaagcaa gtgacctgga tcatagaaac gaatcaaggt
    30781 tccatctcca atcttgcctt gaccaccgta gacatcacct gctggtgagc aatcaactgc
    30841 gaggaagact tctgggtcaa acttggttgt agaggtatga gcgccacgca gaccaacttc
    30901 ttcttggacg ttagaaccca gatagagttc attgccgagt ttttgacctg ataaagcttc
    30961 agctagctcg cttaccatga ggacaccgta gcggttatcc caagcttttg agatgatatt
    31021 tttttcattg gctgtcaaaa ttgcagaact atctggtacg atggtatcac caggacggat
    31081 gccaaaactt tctgcctcag ccttgtccgc aaaaccacca tcaaaaacga tatcggcaat
    31141 ggctggcatg gttggtcccc cctttccacg agtcaaatgc ggaggaacag aacctgaaat
    31201 cacaggaatt tcatgaccat cacgagtcaa gagtttgaaa cgttggctgc taaccaccat
    31261 ggggttccag ccaccgattt ctacgacacg gaaggtacca tctagcttga tttcgctgac
    31321 cataaaacca acttcgtcca tatgagaagc gaccaagacg cgcggtgcat ccacagcttc
    31381 tgaatgtttg ataccaaaaa taccacccaa gccatctgtc accacttcat ccacatgcgg
    31441 tgtcaacttt tcacgaagat aagcacggac aggcgcttca tgacctgaga ctgcagcaag
    31501 ttctgttact tctttaattt ttgaaaataa tgttgtcatt tcagttcctt ctttctttca
    31561 tccattttac cactttttat aggagaagga tagtgggaag gtggatttct aagttagtat
    31621 cttagtcctg ctctatctta gaaaaggata gtattctctt gcatgtagtg caaaatctag
    31681 taaacattcc aaaattaact cgaatattta tttccaaaca aaaaaacaat acaccatcaa
    31741 agttgtttag atttttcatg aaatttacag aaaatagttg acttcccttt cttctttctt
    31801 taaatatata gttggttgag tttggaatag tacgctgtag ctgctaaaac atttctagaa
    31861 attaatttga ctttcctaat agagttgttc atatcttatt tcaatttact atagtacaaa
    31921 actagaaaag gaaaaaatca tgaccaaaac aattcttgtt acaggtggga ctagctacat
    31981 tggctcccat accgttaaag ctcttttaaa tgctggctat caggtgcatg tcctcgataa
    32041 tctctccaca ggaaatcgag cggctgtgga tagtcgtgct agctttaaag aactggatgt
    32101 ttatgatgct agcgccttaa aggcttactt agaagaaaat cagattgatg ctgtcctcca
    32161 ttgtgcaggt gaaattgttg tgagcgaaag tattgaaaat ccaagtaaat acttcactgc
    32221 caacgttgct ggtatgaacc aagttctcaa agtcttatct gaagttggca ttcaaaaaat
    32281 catgttctct tcgactgctt ctctctatgg aaataactgt attgacaagc cggtgactga
    32341 agataccctg ctcgaccctg tcaaccctta tgcagagaca aaactgatgg gcgaacgaat
    32401 gatttactgg atggccaatc gctacgactg gaaatatgtt attttccgtt actttaatgc
    32461 ttctggggct gaaatggatg cttcaaacgg tctgcgtgtg aaaaatccaa ctcacatcat
    32521 tccaaatatc aacaagaccg cattgggaca aaatgatagc ttaaaaatat ttggagatga
    32581 ctacgataca cgtgacggtt catgtattcg agattacatt tatgtcttgg accttgcata
    32641 ggctcatgtt aaaggaatga actacctatt tcaagaagac agttcttctc aaatctttaa
    32701 cttaggaact aaaaaaggct atactatcaa agaaatcttt aaaactgctg aagaattact
    32761 aaattaaaaa atcccacacg agattgttgc tcgacgtgct agtgacccag ccagtgtcct
    32821 agcagatgct tcaaaagcta aacaatatct tgattggacg gctagctact ccctcgaaga
    32881 tattatttta tcagattaag gtaaaaacat tttagaatag gataacaaag gagaaagtct
    32941 tgttgctctc tccttgttat atttattaaa ttgtcagaaa ttttattgac agattagaaa
    33001 aataatgtta catttatatc cgcagatatc tttcgatacc aaatctacat gaaaggacgg
    33061 ggtatgaaac tttctcatta tttaattggc ttacttctac tcctagtctt tctctctatt
    33121 agcattggga ccagtgattt ttcatgggga aagctatttg atttcgacca gcagacctgg
    33181 ctcctttttc aagagtcccg tctcccaaga actatcagta ttctcctgac tgcctctagt
    33241 atgagtatgg caggccttct catgcagact attacccaaa atcagtttgc tgcaccgagt
    33301 acagttggaa cgactgaagc cgccaaactg ggaatggtgc tgagcctttt tgtctttcca
    33361 tcggctagtc tgacccaaaa gatgctcttc gcttttgttt catccatcgt attcaccctc
    33421 ttcttcctag cctttatgac catttttact gtaaaggaaa ggtggatgtt gcctctgatt
    33481 gggatcatct atagcgggat tatcggctca gtcacagaag ttatcgccta tcgtttcaat
    33541 ctggttcaga gtatgactgc ctggacccag ggctccttct ccatgattca gacccatcag
    33601 tatgagtggc tcttcttagg cctcatcatc ctgataaccg tttggaaatt atcccaaacc
    33661 ttcaccatca tgaatctggg gaaagaaacc agcgagagtt tggggatttc ctactcccta
    33721 cttgaaaaac tggccctctt tctggtggcg ctaacgacaa gcgtcaccat gattaccgtg
    33781 ggtggcttac catttctcgg agttatcgtt cccaatcttg ttcgcaagcg ctatggagat
    33841 aatctaagtc aaaccaaact catggtcgca ctggttggtg ccaatctagt tctggcttgc
    33901 gatatcctat cccgagttct gattaggccc tatgagttgt ctgtcagtct cttgctagga
    33961 atcatcggta gtctcgtctt tatcctactt ctctggagag ggggacgaaa agatgcagac
    34021 taaaagcaaa cataccaagc tcttctggat tctcattatt cttgccatcg gagcttgtct
    34081 tctctacttt tggcccatca ctcacttgtc agcctttgct tggaagttgc gttcccaaaa
    34141 gatcatcgtt tatctcttgg tagccatcgc gactgggatt tcgaccatta gttttcaaac
    34201 cctgacggaa aatcgcttcc tgacgcctag tattttggga attgaatcct tctacgtcct
    34261 actacaaacc ctactactgg tttttgaaag caagtttctt caacttggca aatcccctat
    34321 cttagaattc ctagtcttac ttcttgtcca gtccttcttc tttctcgcct tacaaggtta
    34381 cttgaagaca ctgatgaagc aagacctggt cttcatcctg ctgatctgtc tagcgctcag
    34441 aagtctcttt cgaaatatca gcaccttcct tcaagtccta atggatccaa acgaatacga
    34501 taaactgcaa aatagtcttt ttgcctcctt tcaacatctt aacacttcca tcctagccat
    34561 cggttctctg atcatcctcg ctttgacaat ctttttcttt cgaaaagcag tcgttctaga
    34621 tgtcttgcac ctgcaaagag aaacggctca gatattggga ctcgatgttg aaaaagaaca
    34681 gaaagagctc ctctggggaa tcgtgctttt gacctcaacg gccactgcct tggtaggacc
    34741 tatggccttc ttcggcttta tgctggccaa cctcacctac ctgattgtca aagactatca
    34801 gcacaagtta ctctttatag tggccattct ggttggattt attagcttaa ccttggggca
    34861 agccttgatt gaacgagtct ttgcactgga aattcgtatc agtatgatca ttgagagtgt
    34921 gggtggcttc ttattcttta tcttactata taggagggct cgtcagtgaa actggaaaac
    34981 attgacaaat ccattcaaaa acaggatatt ttgcaaggca tttcgcttga agtcagtcct
    35041 caaaaactga cagcctttat tggtccaaat ggtgctggaa aatcgactct cctctccatc
    35101 atgagcagac taaccaagaa agatcaggga gttctcagta tcaaaggacg tgaaatcgag
    35161 agctggaatt cgcaagaact ggcccaagaa ctaaccatcc taaaacagaa aatcaattac
    35221 caagccaaat tgactgttga agaactggtc agttttggac gttttcccta cagccgaggt
    35281 cgacttagat cagaagactg ggaaaaaatc cgagaaactc tgaactattt ggaactgacc
    35341 aacttaaaag accgctacat caatagcctg tcaggggggc aactccagcg cgtctttatc
    35401 gctatggtac tggcccagga tacggacttt atcttgctgg acgaaccact caacaatctc
    35461 gatatcaagc aaagcgtcag catgatgcag attcttcgac gactggtgga ggaactcggc
    35521 aagaccatta tcatcgtcct ccacgatatc aacatggcca gtcagtatgc agatgaaatt
    35581 gtcgccttca aggacggcca ggtctttagc aagggaagaa ccgatcaaat catgcaggct
    35641 gacctgctca gtcaacttta tgagattccc atcacgctag ctgatatcaa tgacaaaaag
    35701 atctgtatct atagctagta acataaaagc tcaagttaga gaaccttcag tctcttagtc
    35761 aataagatta agagactccc taaatcgtta tcacatttta aaaaggagaa attatgaaaa
    35821 catcccttaa actttatttc actgccctag tggccagctt cttgctccta cttggtgcat
    35881 gtagtacaaa ctcaagcact agtcagacag agaccagtag ctctgctcca acagagataa
    35941 ccattaaaag ttcactggac gaggtcaaac tttccaaagt tcctgaaaag attgtgacct
    36001 ttgacctcgg cgctgcggat actattcgcg ctttaggatt tgaaaaaaat atcgtcggaa
    36061 tgcctacaaa aactgttccg acttatctaa aagacctagt gggaactgtc aaaaatgttg
    36121 gttctatgaa agaacctgat ttagaagcta tcgccgccct tgagcctgat ttgattatcg
    36181 cttcaccacg tacacaaaaa ttcgtagaca aattcaaaga aatcgcccca accgttctct
    36241 tccaagcaag caaggatgac tactggactt ctaccaaggc taatatcgaa tccttagcaa
    36301 gtgccttcgg cgaaactggt acacagaaag ccaaggaaga attggccaag ctagacaaga
    36361 gcatccaaga agtcgctact aaaaatgaaa gctctgacaa aaaagccctt gcgatcctcc
    36421 ttaatgaagg aaaaatggca gcctttggtg ccaaatctcg tttctctttc ttgtaccaaa
    36481 ccttgaaatt caaaccaact gatacaaaat ttgaagactc acgccacgga caagaagtca
    36541 gctttgaaag tgtcaaagaa atcaaccctg acatcctctt tgtcatcaac cgtacccttg
    36601 ccatcggtgg ggacaactct agcaacgacg gtgtcctaga aaatgccctt atcgctgaaa
    36661 cacctgctgc taaaaatggt aagattatcc aactaacacc agacctctgg tatctaagcg
    36721 gaggcggact tgaatcaaca aaactcatga ttgaagacat acaaaaagct ttgaaataag
    36781 ccacttcaaa ctcaatttaa tactcaatga aaatcaaaga gcaaactagg aaactagccg
    36841 caggttgctc aaaacactgt tttgaggttg cagatgaaag ctgacgcggt ttgaagagat
    36901 tttcgaagag tataaaattt tgacattcta aaacgcatcc tatcaagttt actcaaacct
    36961 gataggatgc gtttttatca ttttgaaatc tatttacccc acctcattcc ccttcttgat
    37021 ttcgtttaag ggaaaagtgg tctggaacgg gatccttacc tgttttcgac cagggatgac
    37081 aacgtaaaat ccgagccaag cccatcaata cccccttaaa accatgtttt tcaatagcct
    37141 gaatcatgta gttggaacaa gtcagctcaa agcgacaaga gggtggaaag actggtgaga
    37201 taaaacgttg gtaaaagcgc acaggcgcta ttaaaattcg tttcattatt tcttagttac
    37261 agccatggtg tgtagttggc tgatttcttt tttattaaga cgacgggatt ctcctggacg
    37321 gagtcctgtc aagtctaggt gtccgaaacg agtccgagac aacttatcta cttggagacc
    37381 aacagcttca aacatctttt taacctgatg gttacgccct tcatggatgg tcaactgcac
    37441 cacagagcga tttttgactg ggtccacttt gagaatttca taaacagctg gcttggtttt
    37501 cttaccatca atctcaagac cacgggtcaa ggggcggaga ttgtccttat tggccacacc
    37561 tttaacacgc gcgacataaa ccttgtcaat ctcattacga gggtgaatca tctcgtctgt
    37621 aaagtcccca tcattggtca aaatcaagac acctgatgta tcccagtcca aacgacccac
    37681 agggtaaata cgctctttga cattgggcaa gaggtcgaca accgtcttgc gacccttatc
    37741 atctgtcaca ctggaaatca caccgcgtgg tttgttaagc agatagtaga ccttttcttc
    37801 gttgtagata ggttgacctt caacttcgac cttgtcgcct gacttgatag tggttgctag
    37861 ttcacgcacc acttggccgt taaccgtcac caagccttgc ttaatcagct cttctgcttt
    37921 tctcctactg gccacacctg cgtgggcaat atacttattg attctcatct tcttctatcc
    37981 tttcaccaaa taattggctt tcttgggctt gaatctcaag ctcatcaatc actggtaatt
    38041 cttctaaatg gtttatcccc atgtaatcta ggaaataatc cgtagtcaca tagaggttgg
    38101 ggcgccccaa tacttccttt ttcccgtctt cctttatcag gtcaaaagcc tgcaactttg
    38161 ccaaggctcc actcgagtta actccacgga tggcatcaat ttctatccgc gtaatcggct
    38221 gtttgtaggc aataatggac aaggtctcaa gggcagcccg agacaagctc tggttgatag
    38281 gcgccttaga gtattccttc aaaatctctg caaattgagg cttggtcacc aatctataag
    38341 caccacttgt ctcaatcaaa gccaaactgg aatctgggtc cttttcatac ttctgggcta
    38401 attttcctaa actttgctgg atgcctgtcg gtggcagaga gaggagttca gctaactggc
    38461 ggacccgaat cccatcttca cccgctacaa acaagagcgc ttctattttt gctaaagtac
    38521 tcatctttcc tctctatcaa gtctagcttt gaggcacttg actttcttcc ttcttttcca
    38581 tgagatagat atctccaaaa ctctcctctt gcacgaggat caactcctgg gttttgatta
    38641 actctagggt tgccaaaaag agggtgatga cctcttggac attctgggct tccttgaaca
    38701 aatcctgcaa gcgcaattga gctcgtccaa ccaaggactc tttcacgata atcatcatgt
    38761 cctcaatctt atactcatcc cgcaagatcg tcgtgtgatt ttgtgcaaac tcctcttttt
    38821 tcttggctag gatatttgaa aaagccaaaa agaggtcaat ggtcgtcttg tcatgcacaa
    38881 gctccgcatc ttcgtaaatc aactctgtcg gcgctttgga ataatactgg gcccgttctt
    38941 ggtgcttggc ttccaagtgc tcacccaaga gcttgaactt gcgatattct tcgatttgag
    39001 agaggaggtc ctgctccagg tcatccccca agtctgtcac ttctgctacc ttcggaagga
    39061 gtttacgact cttaatcagc atgagctgac tagccatgac catgtactca cccgtcactt
    39121 ccagacgcat ggcctgcagg gttgagacat aggctagata ctgttcgatg acttccgtaa
    39181 tgggcacatc gtagatatcc atctggtact tagaaaccag atgcaagagc aagtccaggg
    39241 gtccttcaaa atcttttaat ttaatatcca ttatctatat ttttctaagg tcaggactgt
    39301 ttttaatcct aatttttttg caatttcgta caaatcgacc ttgttttcta tttgtcttag
    39361 aataaactgt tcacgtaaga cttgagctga taaggatgga aaacctttct ccttgacaaa
    39421 agattctaac tgacgaaagg cccactgacg agaatagggt ttctctcctc tttcaaaaag
    39481 ataggtctgc cccatcaagg gttccaattc tgaaagcaag gccgtgggaa tggtgacaat
    39541 cctctgttgg gaagccttgc tgattcgcaa cacctgaaaa tccagattga tgtccgcaac
    39601 cttgatggct aaaatctcac tgggcaagag ccccatttct aggattaaga gcgctagcaa
    39661 gcggccctct ggatggtcgc tttcctgcca aaaagagtct aggtatagaa tctctggctt
    39721 ttccgtcttc ttttcagctt gtttggctaa ttccaagcgg taaaagctgt ccacctctcc
    39781 tttttgatag agaaagtata gaaattggtt acaggccgaa atctttcgct tctgggcgct
    39841 gatttttaga ttggctagct gggcttggta aatcttgaga ctggtctcag aaatccgctc
    39901 acctaccatg tctaaaaatt gctccaaatc atacttatag gactgcttgg aattgacaga
    39961 caagccctgc ttttcctcta aaaaggctga aatcctgtct ctcattggca tcgaatctca
    40021 tattccttac taaagtcatg caagagggca ttaacggcct tgaggatgga cttgcgcgta
    40081 ataatccctt ggaaaatacc ctctgcatcc acaaccggta agaaggactc atctactagc
    40141 ttgtgcaaga cctccgtaat ggtgaaatca ggcgaaacaa ccgctacgtc cgtttttgtc
    40201 atatgaacga tatccgtatc cgccatgatt tcttggctca agtcatgctc catctgataa
    40261 gccataatat ctctgagtcc aatcgtccca acaaactgtt tttcatctgt cacaacggga
    40321 acacgggtat aggtcatctg actgagcaag agggtcgcat gatccgcatt gtgggtatca
    40381 atcaacacag ctagattttt agcaggggtc aaaaaagttt cctcctgccc caacaagaaa
    40441 gtctcaaact ccttggcaat catcggctaa actccttgga caaacctgga tacacctcgt
    40501 gatctcgtgt caaaaagtcc actttgaagt aactatcatc aatctccaca cgagcataga
    40561 gacattctct gatggtacct cgtggttgac tgatagaacc tggatttaga aagaggatct
    40621 tgccttccat ccaagcactt ggcacatgca agtgaccata gaggcagata gcggcctctt
    40681 cctcctgagc ccagtagtcc aacttttgaa agttgaaatt gatgtcaaac aagtgaccat
    40741 gagtttggat aatcttggtc gaaccaagct cagtcaccag acgttctggg tagccggcgt
    40801 agaagtccat gttcccttta acaacgcgga tgccctccca aagtggagaa tacggacgta
    40861 gttcagaatc gccgttatga aaaacggcat cgactttgcc cacatagcga tcacggactt
    40921 cttccacaat caagctatcg ccatgggaat cgctcattac aatgatagtt tgctttgcca
    40981 tgatggaaat acctccaaaa gtttcttaac ggctaaggca cggtgagatt gactattttt
    41041 ttcttccagg gttaattcag ctgatgactc acctgtctct cctacaagga agaggggatc
    41101 atagccaaag ccattttcac ccttaggttc aaagttaata taacctgacc agtctgcttc
    41161 aacaactaaa ctttccttat ttgggctggc tacgactagg gttgtgtgga actgagccga
    41221 gcggtccttg agttcaaaga ccatggccaa ttcgtgcaag agtttggcat tattttcacg
    41281 gtcagttgct cccacacctg cgaaacgagc tgaccagacg cctggtaagc caccaaggac
    41341 atcgactttg agaccagaat catctgccaa aaccatcttg cccgttaatt gagaaatggt
    41401 ttctgcctta aggcgggcat tttcttcaaa ggtcatacct gtttctgcta cttcaggcag
    41461 gtcagggtag tcattaagat tttccacatc gtagcctaac ttatcaaaga tagctcggaa
    41521 ttccttggtt ttaccttcgt tacgagtcgc aatcaacaag gtttctctga ccttgcttgt
    41581 ctcaaagaaa tcatgaacat tgaccccttc tttaggcaat tctacataca agagttgccc
    41641 attttcaacc aagagcaggg ccccatgacg ttgaataact tccaagtttc gtcccatttc
    41701 ttgattaagc atgtctacca caaaggagaa caaacggtag gtagaaccat agcgtaaaac
    41761 agtaactgaa agcgggaaac catactcttc atctccaaat attttggaga attcaaacag
    41821 aggaaaatct gtcttatagt cgctcaaact gttaacgcca ccaaaaatac tataagaccc
    41881 aacataccag tcctggtcat ccttatattc ataaatttta tttgtcataa ttctacatgc
    41941 tccacatgaa tctctttttc cagccattct tcaccaattt gtgcaaaact ttggctactg
    42001 gctgttgtgt aaaaacggtg atggagtggt ccagcatcgc gaccacgatt gatttcaaaa
    42061 taattgagta agactgagat atcccgtacg cactctgccc cactatcgat gagctgaacc
    42121 tttggcccca tcacattttg gataataggg cgaaggagtg gataatgagt acagcccaaa
    42181 atcaggctat ccacctttcc aaccaaggga cgcagggttt catagaccac cttcttggta
    42241 acactggttg acagggcacc tgactcaacc aagggagcaa acttgggaca ggccaagctc
    42301 tccacctgta agtcgggatc cagatcatgg attttctgac ggtatatgtc tgattgtacc
    42361 gtcatgggcg ttccaatcac tccgattttc ccaccttgac tggacttgat ggctgccgaa
    42421 gctcctggca aaattacacc caagacagga atatctagtt gagccttgat ttcttcccag
    42481 acgaccgcag tcgcagtgtt acaagcaatg acaatcattt tgacatcctt ggtcaagaga
    42541 aagttgacca gctgccaagt atattcacga atttgctcag caggacgggg gccatagggc
    42601 gcccgcgccg aatctccaat atagacgatt tcttcatggg gaagctggcg catgagctcg
    42661 cgcacaacgg tcaagccccc gacacccgaa tccaaaaaac caattggtcg attatccata
    42721 cagtcttctt tctagtcttt tttctgattg atcgttcatt atgattcctt gtactgaacg
    42781 aactgaccga cagcttgata gaatgtcaac tacctctctc ttaatcataa tcaaatatca
    42841 atagaatgca aggaaaaagt ctcatcccta aaccataacc aacatagtga gaagtctgga
    42901 actttcatcc cagacttttc cttatttatt ttttcttttt attgttagca agggctgcct
    42961 tttgttggcg gatgatttgg tggtaaactt gttgtacctt agcttcgctt ggtttttgac
    43021 catttgcact caaaagagta cgaactgctt cagcattcaa acgtgggttg tcagcaaatt
    43081 ctttttcgat ttgcttacga accaagtaca ttcctccaag agctcctcct agaaaagcta
    43141 gcacaatcaa tacaattgct aaaagtaaat ccatcatttt tctcctgttt ctttttgaat
    43201 ccctttcatt ataccacaaa ctagccaccc tgaccagttt gttctacact ttcagggatg
    43261 gaatagacag caaaaagaac cctgcttttc tgaaaaagag gtctccttgc taagtttata
    43321 ctcaatgaaa atcaaagagc aaactaggaa gctagccaca ggttgctcaa agcaccgctt
    43381 tgaggttgca gataaaactg acacggtttg aagagatttt cgaagagtat taatttacat
    43441 aaatagccag tgtttgatag ggtttgagta gaattttctc agacacttct gcatcttcat
    43501 agtttgatat caaaatctgt ccattttggt agactgctgg caagtcgatt tccacttctt
    43561 tagcataaaa gttattgagc actagtaact tttgatcctc aaactgacgt tcaaaagcgt
    43621 agacttgttt gctatcttca aaggctggtt tgtaacttcc ttctgaaatg attggcattt
    43681 ccttacgcag tcgaatcaag tcttgataga aggtaaaaat cggaccctgg atttcatttt
    43741 ctacattgat atctttatag gatttaccag ctttcaacca tggagtgcct gttgaaaatc
    43801 ctgcattttc cgaagcatcc cactgcatgg gaatgcgtga attatcacgc gacttagctt
    43861 gaataatctg gaaggcttct tgctgactct ttccttcttc taagagcatc tgataggcat
    43921 taagcgattc gacatccaca taatcagcca tagaatcata gtctgggtca atcatcccga
    43981 tttcctcacc catgtagata taaggtgtcc cacgtgacag gtgaatgctg gctgctagca
    44041 tggtggctcc ttccttgcgg aagttttgaa tatcgacaaa acggttcaag gcacgtggtt
    44101 gatcgtgatt gttccaaaag agggcactcc aaccgtcttt atcactcatt tccttacccc
    44161 aactatggta aagactcttc aactcttcaa aatcaaaggg agccaaggtc cacttttgtc
    44221 catccttata gtccaccttg aggtgatgaa aattaaaggt catggataat tcctgacgat
    44281 caggcgacga atagaggaca cagttttcca tggtggtaga agacatttcc ccaactgtca
    44341 taaagctatc gtcagatcca aaagtggctt ggttcatcat acgcaaatag ttatgaacga
    44401 tgggtttgtc tgtataagct ggcttccctt cattttcagg acagtccact gaaacctcgt
    44461 ccttaccgat caaattgatc acatcaaatc gaaaaccttt gacacccttg tcgcgccaga
    44521 aattaacaac cttgaaaagc tccttacgga cattgggatt gcgccagtta aggtcagcct
    44581 gggtctcatc aaataggtga agatagtatt tcccagtatc cccgaaaggc gcccatgcag
    44641 aaccaccaaa cttagactgc caatctgttg gttggtcttg gatgaagaaa aagtcttgat
    44701 aatacttatc accagctagg gctttctgaa accattcatg ctctgtcgaa caatgattaa
    44761 gtaccatgtc cagcataaag tcaatcttgt gctctttacc gacacacacc atttcctcaa
    44821 aatcagccat atcaccaaaa agaggatcca ctgccatata atctgaaata tcgtaaccat
    44881 tatcccgttg agggcttgga tagaatggat tgagccagac catatccaca cctagtttgg
    44941 ctaaataggg aattttttcg ataatcccac ggaaatcccc aataccgttt tcagtggtgt
    45001 ctttgtaaga ttttggatag atttgataga ctacttttcc tttatcaagt gtcatctgtt
    45061 tctccttttc tgataaaagg gaggaagcag tcttccgtcc ctatttgtgc tatttcaatt
    45121 atactcaatg aaaatcaaag aacaaactag gaagctagcc acaggttgct caaaacacta
    45181 ttttgaggtt gcagatagag ctgacgtggt ttgaagagat tttcgaagag tattagattc
    45241 gtgtagcgac catgagagat gctccagctt ggatcgttgt cggataagtt ccagtaatag
    45301 tcgctgtata agcatcttgg ttggtgatga taacaggagt ttctgtcacc agacctgcag
    45361 ccttaatgac atccatatca aaacgaatca gttgctggcc aactataacg tgatctcctt
    45421 ggactacaag actttcaaaa cctttgccat caagacctac tgtatccata ccgatgtgga
    45481 tgagcaattc aactccctcg tcagagacaa tgccgatggc atgcttggta gggaaaagaa
    45541 ccgtcactgt tccattaact ggagaggtca actcaccttg gcttggttca atgaccagac
    45601 cttgccccat gacacctgat gcaaaaacag gatccgtcgc ttgactcaat tctttcactt
    45661 ggccagttag tgggctgata atttctaccg aagtaagttc tactggttca tggctcacaa
    45721 attctgcttc ttcttgagca acgaattctg cctgcaagtt cgtatcgccc tctatttttg
    45781 tgaagagacc agccttgcga aagaagaaag tcaagagcat tggaacaaca atcgcaacta
    45841 gcatagttcc tgcaaatggc agcatgtatt gaggttgaat agagagaata cctggcaaac
    45901 caccgatacc aatagaagcc gcagttacat taaaagtaac ggataacatg cctgcaaggg
    45961 ctgagccagt catcccagca acaaatggat aaatatattt tacgttaacc ccaaaaagag
    46021 ctggttctgt aacaccgaga taggctgaaa tggttgcagg aagtgaaacc tgagcctcac
    46081 gctcatcatg gcgatgcatg aaataatagg caaacacggc tgagccttga gcaatattag
    46141 aaagagcaat cattggccat agggcagtgc caccagcatc cgcaatcaat tgtgtatcaa
    46201 tggcattggt catatggtgc agacctgtga tgacaaatgg agcgtagagg gcaccaaaaa
    46261 ttgcaccgaa gagccattta actggaccag ttaaacctgc caagacaact gatgaaagtc
    46321 cttgtccaat tgtccaaccg attggtccca aaacagtatg agccaaaatc aaggctggaa
    46381 tcaatgacaa aaaaggtaca aaaatcatgg aaatgacttc tgggatatgt ttgcgccaga
    46441 aaatttcaag ataagacaga ctcaaacctg caagcaaggc tgggataact tgggcttggt
    46501 aaccaatacg attaacagta aaataaccaa aattccaaac ccagtttgcc gcgatatcag
    46561 ctgctggcgt tgaagcaacc gcataggcat tgagcaactg aggcgatacc aaacagattc
    46621 cgagaacaat ccccaaaatt tggctggttc ccatcttacg agaaacagac caagtaatcc
    46681 ctactggtaa gaactggaag atagcttcac caggcaacca gaggaagtga ttaacccctg
    46741 cccaaaactg agaggattct gtgatggtct tgccatccaa catcgaccaa tggacacctt
    46801 ccaagacatt acggaaaccg aggatcaatc caccaacaat caaggctgga ataatcggag
    46861 taaagatttc cgccagagtg gtcataacac cttgaaccac gttttgatta ctcttagctg
    46921 cagacttggt tgcttctttg gaaacaccct caatacctga aacggctgta aaatcattat
    46981 aaaagatggg cacgtcattt ccaatgatta cctgaaattg acctgcattt gtaaaagttc
    47041 ctttaacagc tggaattgac tcgatagctt taacattagc cttcttatca tctcttaaaa
    47101 caaagcgcat ccgtgtcgca cagtgagtta cggcagtcac attttctttg cctccaattg
    47161 cctgaagcag atctttggct tcttgttcaa attttcccat ttgattctcc ttatacttgt
    47221 caaagcaagt ttgatttgat aagtatattg taaacgatac caaacttgtc tacaacttgt
    47281 ttggtataaa aagggtgttt ttttgtaaat tttctagcac ttgtatggta aaatagatac
    47341 aggtgttcat taaactagac taaaaaccta tttaagcagg caaaatgaag aaataccaac
    47401 aattatttaa gcaaatccaa gaaaccattc aaaacgagac ttacgctgtc ggagatttcc
    47461 ttcctagcga gcacgacctt atggagcaat atcaagtgag tcgtgatacc gtccgaaagg
    47521 ccctgtctct cctccaagag gaaggattga tcaaaaagat aagagggcaa ggttctcaag
    47581 tcgtcaaaga agaaaccgtc aatttccctg tatccaacct aaccagctac caagaactag
    47641 ttaaagaact tggactgcgc tctaaaacca acgtggtcag tctggacaag attattattg
    47701 ataaaaaatc ctcactgata accggtttcc cagagtttcg gatggtttgg aaggtggtcc
    47761 gccagcgtgt ggtggatgat ctggtatccg ttctggatac agactatctg gatatggaac
    47821 taattccaaa tctcactcgc caaattgctg agcagtctat ctattcttat atagaaaatg
    47881 gcctcaaact ccttattgat tatgctcaga aggaaatcac cattgaccac tcaagcgacc
    47941 gagacaagat tctcatggac attggcaaag acccttatgt cgtttcgatt aaatcaaaag
    48001 tctatctcca agacggacgc caatttcagt ttaccgaaag tcgccataag ttagaaaaat
    48061 ttagatttgt agattttgca aaacgcaaga aataaaagac tgagacacca gatctcagcc
    48121 tttttcttta tcccaaaact aattcatcac tgaccagact ttggccacta tttttctgga
    48181 agagatgcat aaggtcttca actgtcagat gctttttctc ttctcccttg acatctacca
    48241 ctatctttcc ttgatagagc ataatgagac gattgccgta ctcaatcgca tgattcatat
    48301 catgggtaat catcaaagtc gtccactgat ggtgctccac aatcttttgc gtcaagttca
    48361 tcaccatttg actggttttc aggtcaagtg ccgcagtatg ttcatccaaa agtaaaagtt
    48421 tgggtttcac cagagctgcc atgactaggg tcaaagcctg tctttgtcct cctgagagat
    48481 attgagtatc tacttttaag cggttttcaa gaccaatatt caactccttc aaggcctctt
    48541 ggaactggat tctatccttc tccttcacac cccaaccaag ccctctcttt tgctcacatc
    48601 tcaaggcaat agccatattc tcctcaatcg tcaaacgaga agctgttccc atcttaggat
    48661 cttgaaaaac acgggcgata tcattggctc tctttcttac attcagatgt ttaatggatt
    48721 tccctgccaa taaaaggtcc ccctcgtcca ccgatagatt gccagccaag atattcatca
    48781 aagtggattt ccctgctcca tttccacaaa tcacagagat aaaatctccc tcttcaacct
    48841 ctaaatctaa tcctttgagg acatggttct cattgaccgt ccctgcttca aatgtcttgt
    48901 gaatattttc aagtgttaac aaacttgcca taactttctc ctcctattca tttctcaatt
    48961 tcagaccacg aatctttaat ctcttttgca attctggtgc aaataaaacc aaagcgagca
    49021 agattgcatt aaagagacgt accaagtttt gatctagatt tggaatttca tagatattta
    49081 aaataatcaa acggtaaaca atagcaccga ttccgatgga taataagcgg cctccaatgg
    49141 tcaagtcgtg tatcaagact tccgagctca aaccaacaac aatggttcct gtcccagaag
    49201 tcacatctga aaatccatca ttttgggcaa acaaggaacc acatagggca atcaaaccgt
    49261 ttgaaatcat gtaaccaaca atcttcatgg tgtttacatt gactccattg gcctcactca
    49321 tcgggatatt gtccccagtc gaacgcaaga ccaaaccaat ctctgttttc atcaaaaggg
    49381 tcaagaccaa acaagtaaga gacagactaa actaagtgag aaaacagctc cttcatttgt
    49441 cagacccaag ctcgccaact gtttaaagac ggttgcagaa tctcccaagg aaagattggg
    49501 tacactgccc ataattttaa tattgattga atagagccct gtcaaggtca caatccctgt
    49561 caagagagct gggattttca tattggtgtg gagcattcct gatacaagac ctgctaccat
    49621 acctgccacc aaagcaagta aagtcgcaag ccaaggattt gcccctgcct gtatctgtga
    49681 tacaacgact gctgccccca gaggaaaggc tccctcagca gtcatatccg caatatccaa
    49741 aatacggaaa gtcaagtaga ccccaattgc cataatcgac cacaacaaac cttctgataa
    49801 actagataac acaaaattca tcttaatcct ccttattcct tgccttctaa cttactaata
    49861 tcaattccca atgcatctgc catttcttga ttggtatgca attccagttt ttcaggcaac
    49921 tcaactgcta tattttctgg ttcctcacct tttaaaacac gaatcagcat gcgagctgtc
    49981 tgtcttccca attcttcata attggttccg tagttataca agccaccaac agcaatcatt
    50041 tctgttgaac caccgaatac tggaaccttg tgtttaatag aaacctgctt aactgtttcc
    50101 atggttgacg aaatgatatt atcagttggg acaaaaacca tatccacctc tgccatcaaa
    50161 ctctctgctg ccgccttgac gttattactg tccaagattg ttttttcaac aacggtaaag
    50221 cctttttctt tcagaagtcg cttagcttca tccttttaaa caaccgaatt gggctcgctc
    50281 tgagtataga ggattccaat cgttttagcc tttagcaaca ctttctttat caaattgatt
    50341 tgggttgaaa tggcatctga tgactgatcg cttgtcccag ttacattgcc cccaggatgt
    50401 tctcttgact caaccaactt ggcactgact gggtctgtta cagctgaaaa gataactggt
    50461 gtcgtttgcg tcgtattggc caagctctga gcagaaggcc ttgcgatggc tagaacgaca
    50521 tcgctggatt ctgctagttg ctgggaaatc gtttttagat ttctttgttc tccctgtgca
    50581 ttttgcaaat caatctcaat attttccccc tcaacatagc cttgtttggc cagctcatcc
    50641 acaaatcctt ctctagtagc gtataaggac tggtgggtaa taaattgcga aataccgata
    50701 cgaaagacat ctttttctta tctgccttca actgatgcaa actgatcaga gaagtcaaga
    50761 ggagccccac taccaagagg gggctagtga ttttcgaaca actttcataa tgaaactcct
    50821 tctcagaaaa gataaaacaa aaaaccgcct agatactatc taaacggata cttgaaataa
    50881 cctaacaagt tacaacttag ctagtccatt tagatattca tctatatgga ccacaatcaa
    50941 aaatcttagc cacatagata caaacaaatt gcttgaactg tttgcatcca tggtgacatg
    51001 tctacaaaca ctatctaaag tagctaaagt aaaagttttg attcatagtt acggcttgtc
    51061 tcttattcat ttctttttct gctttctttt ttgatgcttc ctatcctacc acctttttca
    51121 tcacctgtca ataatatttc agaatttttt aaaatttttc gaaaattctt tcaggaagct
    51181 ttcaaagttt ttcaaatcag tccaacaaaa aaagttttat aatttccata aaaattgaca
    51241 tggaaattat aaaaccatta ctagtttagt cctttttgat aacgtgccaa ttcggcttgg
    51301 ttcgcccaaa catagtgacc tggacggatt tctaccatag atggcttatc agtctcatag
    51361 tcgtgttgac ttggatcgta aaccttcaag accttcttac gttccaagat tggatctggg
    51421 attggtaccg ctgaaagcaa ggcttgagta tatgggtgaa ttggattgtt aaacaattct
    51481 tctgtttctg caacctctac aataacaccc ttgtaaataa ctgcgatacg atctgaaata
    51541 aagcgaacaa ccgacaagtc atgggcgatg aagagatagg tcaggccgag ctctttttgg
    51601 aattttttga gcaagttcaa gacttgggca cgtacagaaa cgtccaaggc tgaaattggc
    51661 tcatctgcaa taacaaagtc tggttgcatg accaaggcac gggcaatacc gatacgttga
    51721 cgttgaccgc ctgagaattc atgagggtaa cgagtcaagt gctcagcaag aagacctact
    51781 tcacggataa tattttgaac tttctcttta cgttcttctt catccttaaa taaacggtga
    51841 ttgtaaagac cttcagaaat aatataatca acagtcgcac gttcattcaa acttgcggca
    51901 gggtcttgga aaatcatctg gattcgacga atcaattccg cagcttgttc acgcgatttc
    51961 ttaccattaa tcttttgacc atcaaaaatg atatctccat tacttgtatc atttagaccg
    52021 atgatagcac gaccaatagt tgttttccca ctaccggact cacctacaag cgagaaagtt
    52081 tctcccttgt tgataaagaa gttagcattt ttaaccgcga caaacttctt acttccttca
    52141 ccgaaggaaa tttctaaatc tttgatttct actaattttt cagacatttc cttcctccta
    52201 gtcagccaga tgggcaaatc ccattttttc acggatctta tcatggagat ttgcaatcac
    52261 agctggtttt tctacttttg gagcatcctc atgaagaagc caagttttag cccaatgtgt
    52321 ctctgatact gagaattgag gagctttttg ttcgaagtca atctgcattg cgtagtcaga
    52381 acgcaaggca aaagcatccc ctttcaggtc agtataaagt gacggaggtg ttcctgggat
    52441 tgagtaaaga tcccctttat catcagcaag ctgaggcaag ctagacaaga gactccatgt
    52501 atatggatgg cgagggtcat agaagacttc ctcaaccgtt ccatactcaa cgatttctcc
    52561 tgcatacata accgctacct tatccgcaat acttgccacc acaccaaggt cgtgggtaat
    52621 aaagattgtt gtgaaatgat actcgttttg taaagatttt agcaaatcaa taatctgagc
    52681 ttgaatagtt acatccaagg cagttgttgg ctcatcacag atcaagacat caggtcggca
    52741 ggcaagggca atcgcaataa cgatacgttg acgcattcct ccagaatatt ggaatgggta
    52801 ttcattaaaa cgtctatctg cgtctggaat gccaacctta ttcatgtagt caatggccaa
    52861 ttctttcgct tctttagctg tttttccttg gtgttttaca ataacttctg taatctgact
    52921 accaattgtt ttaatggggt ccaaactagt cattgggtcc tggaagatag tcgcaatctt
    52981 agcaccacga atttgttccc aatccttgtg agaagataaa gctgtcaagt cctgaccacg
    53041 gtagtcaata ctaccttggg caatacgacc attttcttcg agcatacctg tgaaggtctt
    53101 tgtcaaaaca gatttacctg atcctgactc acctaccaag gctaatactt ctccttcgac
    53161 tagttcaagg gaaacgccgc gaatggctgt caatactttg tcacgaacgt caaattccac
    53221 gacaatatcg cgagcagtca aaattacatt tttttctttt gtcatttcta ctcctatcta
    53281 tgtgtacgtg gatcactagc atccgctaag ttttgaccaa ctacgaaaag ggacaaggat
    53341 accaagacaa gggttgtcaa tggaatccag aacaagtaag cattggttgt tacgttttgt
    53401 gaataatccg aaatcaaacg acccaaactt ggcactgtaa tcggtaatcc aagaccgaag
    53461 aaagacaaga aggcttcgta tgagataaag cttggaagca tttgagtcat ggttgtcaca
    53521 ataacagata ccaattgagg catgatattt ttggcaacaa tcttcaaggt tggtgttccc
    53581 aaagtacgtg acgccaagtt gtattccaag tcacgatagc gcaagatttg cacacggatc
    53641 atgaaggcaa taccaatcca tgttgttacg ctcatggcaa aaatcagatt ccagaatcca
    53701 gctccgattg agtaagtcaa gacaataaca atcaaaagag gtgggatgtt tgagatgacg
    53761 ttgtaaactt ccatcatgac acggtcaact gattttgaaa taccccaaat accaccgaca
    53821 aaaacaccga taaccaagtt aatcactgtc gcaatcacag aaatgaggat ggagttacga
    53881 gctccgaacc agacaccgtc aaagagcgat ttaccgttac tgtcagtacc gaaccaatgc
    53941 tccgcatttg gcttgatata acgaacacta aagtcgttta ccttactgac atcattgaaa
    54001 tcaaacttag aaaacattgg gtagatgaaa cttatcaaaa tgatggctac caagattccc
    54061 aacatgacta cagttgattt tttcttcata aattgtttaa acactgattt ccagtaagaa
    54121 tatgctggcg catcaatagt ttcagaggca aaatcgtcac gttttacaaa ctgaaatttt
    54181 tctttatcga ttgtagacat tatttgcctc ctttctcagt caatttaata cgtgggtcaa
    54241 taatagtcat ccaaatatct cccaaaagac gtgagaagat agaaatacat gtaaagatga
    54301 agacaagacc aacgaccata gagttattag atgcttttac agagtcaatc aacattttac
    54361 ccatacctgg gaaggcgaag actgtttcag taagggttgc accaccgata accccaataa
    54421 tggcaccagg aattcctgaa accagcggaa ccatggcatt tttaaagatg tgtttgtttg
    54481 aaatttcttt ttcagacaaa ccttttgcac gagcgaaacg aacaaagtct tgagattgca
    54541 agtcaatcat gtaacgacga atccaaatgg ctgtaacagg agcacccaac aaaccaagga
    54601 tgactgctgg taaaacgtaa gaacgccaat ctccagctcc caagataggg aatgaatctg
    54661 gaagggcaat agatgatcca atcaatcgaa cgatgtaaac caaggcaatc gttggaagag
    54721 caagcaagaa ggtcaaagtc cctgttgaga ggctatcaat ccaagtgttc ttgaaacgag
    54781 ccatggctga accaagtggc acggcaagag cataggcaag aaccaaacca atcaaaccag
    54841 taatagcaga gctgacaatc atagatggat attggtaatt actttcagtc gctgtataag
    54901 gatcatcttt cccatagcta gctacttcac gagagtcagc ctgactaggt gacttgtagg
    54961 ttcttgagta aatatttaca gaagacgttt tcttacctgt tgggaactga acttgggcag
    55021 ttttggtttg tccttgacct tgagtaataa cctgaagaac tggtgtatta gcataggttg
    55081 ggtaagagtc acctaaattc aagttcacaa agttttgatg aacaaatggg aactgactgt
    55141 taaagtacaa gagatattta tgtttagttc ctgaaccgac caatgaccat ccgatagctg
    55201 gatcattttc aaaacgaagg tagcgtttca agtctggatt ttcagggtct tggattttat
    55261 ttgtatggtc aatgtcaatc aagttagcat agaagtgaaa aacacgttca aaaattggaa
    55321 tttcacgagt agcatagaat tgaccacttt cagtaaattc tcccaaagtc caaccatgac
    55381 ctaattgatt gatgtacttt tcataaatag ctttattggt cgcatttgct tctactgtta
    55441 cagaagaatc catgctactt gccttttctt gcaactcttt agtatcgtaa tactcaatgt
    55501 agcccatacg ctcaaacaca gtattttcat agttatcacg tttatcagcc gttgtcgcaa
    55561 ttttattata gttaggatcc tgcttgaaaa tcaattttcg aggaaccaag gtatagataa
    55621 tcgtgtaggt caaagtcgtt actaagaaaa tcgaaaccaa tgaccgcaaa acacgcataa
    55681 aaatatattt tttcatatta tttcctttaa aaatcccaaa agaaccttct cctcatggag
    55741 agaaagttct attagaaatt atttacttca catgacttgc caattctttt tgagctttct
    55801 catttgattc agctttttct ttcaaccatt tttcacgagc tttttcatac tcttccttag
    55861 tcaccacttt atcttgtaat ttcaaatatt tgaagtaaac atctgacccc ttagagcctg
    55921 tttgcgcaga agctccagta aatggaacaa ttcgtgaaag cactggtgct gcaccagaag
    55981 aagccatagc aggaataaag agtgaactat ctgtcaacca tgcttgagcc gctgcatatt
    56041 tttcataacg gacattcaag tcgcttgtct ctttggcagc ttcatcaact aatttatcgt
    56101 attctttcaa accaacttga actactgaag ggctatttgg attatcaaat cctaaatatg
    56161 tttttgtagt ttcactgcta gttgttttta aaatatccag gtaagtagat gggtcttgat
    56221 agtctggccc ccatgaaact cctcccgata catcccaatc ctcagatgaa gcattggcag
    56281 catagtaagt aatattaagg aattcatcac ttgtcatttg ttgaatatca acaacgacat
    56341 tttcaacacc aagaactgtt tctacagatt gtttaaagga ctgaatacga gatatgtagt
    56401 tttttgatgc ttggtctact ggaacgtcca gatgaatagg aaactgaacg ccgtctgctt
    56461 ctaaagcttt cttagctttc gcaaactctg ccttggcctt gtcagcattg aataaaccat
    56521 cctgcccatc agctaaattc acacctttcc actcatcacc ataagcagga agttgagcag
    56581 cgactaaatc accaaaggtc ttctcaccag ctgaaacaaa gtctggtttt acaaataaat
    56641 tacgaactgc taaagctgct ccatctttac cattgatttg agctgagtaa gctgagcgat
    56701 caagagcaaa attcaaggct tgacggaaat ctttgttaag caatgccttc ttagtagcta
    56761 ctttctctga atctgtagtt ttagaagtat agttgtaact ttggcgatca atattcacac
    56821 ccagaccagc aatcccagag cctgattgtg tgtaatagat attgtccttg tattcttctg
    56881 caaccttaga atagttggag ctggtagggt aaagacgggc ataactataa gctccactag
    56941 tgaagttacg ctctagcgac tcctgatctg atccatcata gtaagctaga ttgatagtat
    57001 ctaggtggac attttcttta tcccaatatt gctcattttt tacaaactct acagaagatt
    57061 ttgcagtcaa ccctttcaac aagaatggac cattataaag caaggatgtc ggatctgttg
    57121 gtttagcaaa atcgcttcct tttgatgttt cgaattcttc attcagaggc cagaaaatag
    57181 aataggtcaa cttagagttc cagaacggtt caggctggtt caaagtgtat tgtaacgtat
    57241 aatcatcaac cgccttgaca ccaactgttg aaaaatctgt tgaagttcct gatagataat
    57301 ctgccaagcc tttaaccgaa ttttcagcta aatacatagc ttctgatttt ttatctgctg
    57361 cgtgttttaa accgttcacg aaatctttag ccgtcacctc tgcatattct tctccatcag
    57421 aggtaaacca tttaacccct ttacgaatct tataagtgta ggtcaaacca tccttagaga
    57481 cttcccaatc ctctgcaact gcaggagcaa gattaccgta attatcgtta gtgaataaac
    57541 catcaatccc atttgaagtc actactgttg tactattttt acttgaaatc aggtagtcca
    57601 aggtttctgg gtctgctgta taaacatagc cataagcttt aggggctgat gaatcagatg
    57661 attttgaaga actgcatgct gcaagtacac ctgctgctaa taaaacaaga cctgctgtag
    57721 caaatacacg attttttttc attttctact cctctgttta tgtgaattat agattgacaa
    57781 ccattatatc acattatcca ttaaaaatca aacaaatttt cagaatattt aggcttgttg
    57841 gcacaaattt ttcatttttt ttgaatatat gattcaaatt gtcgttcgaa gtgtcaaaga
    57901 ctacagtgaa aataggaaat ttgacgcaga aactttggag tttaggaaga catacagtaa
    57961 aatgaaatac ggacggaaca atgtgatttt ggaattcaaa ttaaattata acaatattgt
    58021 agaagtatca ttctagtatt caagattcag tttactatgt cttttcacac caaccttatt
    58081 ccgaattcaa ttacttttgt gatttacata tatagattaa gactatcttt tatactttaa
    58141 aatttctcgc taccttatcc actatatgct cctcgctatc acgtttctat tcatagccta
    58201 cgatttcact attgctttct ctgacaattc ttatttcctg cgtcagactt aaaacgatct
    58261 atccccagac cattttaatc cgctacctca cgacagtcag gcttggggag cgctattgta
    58321 ttcaccggta gtggatccct acagaggact tacacctcag atgcacgaca tgcccatagt
    58381 ataaaaaaat ctcctaccca aggtagaaga tttcaaactt ataaaactta atccgtcatg
    58441 tccgatacca acattcgatg ctccaatgga atactgcaca taactagcaa gaaaataaag
    58501 cctgactgaa tccagaagag agccaagtca aaaattccgt gcacagcaac cactgtaagg
    58561 aaagatagat aaaggccgat aatcggacgt ttccccgact cctgactcat atccatcatc
    58621 aagcgaacag gagcaacaga agacaaaact aataaaatgg tacccacaat tccgtaactc
    58681 agaatcgtat caatataaag actgtgggca tgttcatgat aaggagcatg tatccgagga
    58741 taagagtgca tataggtcaa tggcccttca ccccaaaaag gattttgctt aaacaaggcc
    58801 atcccagcat cccagataga aatgcgttct tccatagaag agtctaaagt acccattcga
    58861 actcccaaat cactagaaaa gaggaaactc aaaccaatcg cgaagacccc aatactaagc
    58921 caaaaggcct tccagttttt aatagtcgta aagagataga taattgctcc agcgataata
    58981 gcaggaaagg cagttcgatt ttgagtaaag ttcaaaccaa agagattaac aaagcctgca
    59041 atcacacaga atactttcaa ccaattcaac ttggtcgttg taaacagata gaaagcaatc
    59101 ataatacaga aacaacaaat aattccataa taattaggat taaagaaggt cacttctgcc
    59161 cggttctgat gccacacctg catattgggt gaaagaaaag catagttaaa tttcttcaca
    59221 atttggaaat gttctaaact ggcaaaagca gctgacaaga cactaccaaa caagacgaac
    59281 tgcaaaatca atcgaaagaa tttatgggat aaaatcgact gatagtgcaa aaagaaaata
    59341 gtaaatagaa acattcctac tgaagccaca agacccatcc aattttgtgc aagaatggat
    59401 ataacagtac tatagctaag aaaaagaagc agcatcggat gctcccccat tttctgaaga
    59461 atacttttca tgtctcctgt aaaaatcaaa ctgataatat ataaacagag tacaactaca
    59521 aaaagataaa agggtaaaaa gatacttagg ataattccca ataaaatcag ctctttacta
    59581 gacaacccct tcagcttttc aataaagcct attgatttca aaatgaatcc tttctctcca
    59641 aatcagctga ttcagataat agtaagctat cctatattgt accacttttt tagcaatttg
    59701 aaaacaaagg aaacgttttc caaaataaaa accctatttt atccaccata tcaaggcttc
    59761 aaaatgatac ttcaactcca ttctcaatta cccgataagt ctgattttgc aaatcaattt
    59821 ctactactgc tgttacggac ttatctttat tttgacgttt gattacaatg ctgtgagctg
    59881 ttggtgtctc tatctcagta gtcccttcta gatcaaaggc ttctgaacgg ttacggaaag
    59941 aaaatagatt gagaagggcc ttcacaacag gtcgttgcac ttcttttgct atttcctcgt
    60001 tgctatagta atgacgatta atatttcgac cttctttagt ttcttctaat aatttcaagt
    60061 cattcttgcc tgctaataga cccacatagt aaacctgagg aatacctggg gcaaaagctt
    60121 gaattagacg agcgagaaaa tacttgacat catcatctcc aagcgctgaa tagtaggttg
    60181 aattgatttg gtagatatct aagttgttat actcggcact agagtactta cgtttgacat
    60241 tagctccaac cttatagagt tcatttgaag catagtcaat ctcctcatcg gtcaggatat
    60301 ccttgacatc tactactcca atcccatcat gggtatctag cgtcgtaaat tgcttcatcg
    60361 ggctcatctt caaccactta gccaaacgct ctgttctgga actgtaaaga gtataaagtg
    60421 tcaccattgg aagagtaaaa tcataaacat agtaatcatg gtctgctatt ttaaactgaa
    60481 tcgaatagtg ttcatgaatc tcaggtaaaa gctctgtccc atactcagca gcgatatctc
    60541 gaactttgtc caataaatcc caaatatctg gttccacaaa gaaatcatta gtatccaatt
    60601 tcttcactgc ataagcaaag gcatctagac gaatcaaatc acacccatta cttgccaagt
    60661 gctgaatggt cttacggata aattccatag ttacttcttt ggtcacatca agatcaatct
    60721 gctcctcacc aaaggtattc cacaaatgtt ccactgaacc atcttcaaac acaatctctt
    60781 gctttggtgc acgatcctta cgcttgtaaa ttaaatctac atcagactgt gtcggacggt
    60841 tttctggcca aaacttatcc cagtttaaaa agagatcttt aaattcactg gcttcatgtt
    60901 tttcttgata gtccttataa tacttggatt gacgagaaat atgattaatc ataaaatcaa
    60961 acataagata atatctctca cctaaacgct taacatcctc ccaatcacca aaagctgagt
    61021 ccacttcgtc gtagtcaact ggcgcaaatc cacgatcacc tgttgatggg aaaaatggta
    61081 aaaggtgaac tcctccaata gcatctccaa aatgctcttc caaattatca tataagtctt
    61141 taagattatt tccaaggcta tcagaatagg taatcaacat ggttttattt tgaattggca
    61201 tcattactct cctttttcta attgaagcca agtctcatat gatctggctt cataaataaa
    61261 attcatttta aatctctatt tatcatcaaa ctcgtactaa tatagactgt gataaacaaa
    61321 gtactacttt cttgttttct acatagaatt atcaacaagc tgaactcttc ctctgtgtca
    61381 aagactatag attccatgag ctcttcttat actattcgaa aatctcttca aaccacgtca
    61441 gcttcacctt accgtactca agtacagcct gcggctagct tcctagtttg ctctttgatt
    61501 ttcattgagt attacttcac tgccccgttg ctcattcctg aaatgatatg gcattggaag
    61561 aagagataga caatggtgat actgataatg ccgaccacgt aagaggcaaa gcttggtccg
    61621 tagtcgttga aatattggcc tgcgtagttg tattggaaca aaggcagagt ccacattttg
    61681 gaatcccggt tcaagacaag gagtggcaac atgaagtcat tccagaacca aagggcattg
    61741 atgatcatgg ttgtcgcatg catcggtttc atcattggga agatgatgcg gaaataggtt
    61801 gtaaattgat tagccccatc gatctctgct gcttcatcca gactttctgg aatcgagatt
    61861 ttgatatagc caacatagag aaagagggtc tgtggaatcg cataggtcaa gtagagcaag
    61921 atcaaaccaa aggtattagc caaaccgagt ttactcatca taaccgtaat cggaatcatg
    61981 atgacttgga aaggtacgaa gattccgagg attaagaggg tatacatgat ggtaaaggct
    62041 tttcttttac tcatattgcg agcgatggag taggctgcca tagggataaa gatcattact
    62101 gcaagtaaag acaagacagt gatgacgaca gagttccaat aatagcctcc aatcccatca
    62161 gctaagagac ggctaaagtt gtcccatgtg aagttggttg gaaagccaaa taaattatct
    62221 acaatatcct tagtgggttt gaaggaacta aagagggtag caaggagcgg cactaaaatc
    62281 agaaccgatc ctagaatcaa tagaatgtat ttgccaatca gggcttttct ttcatcttgt
    62341 ttcatcatgc ttctcctctt aaatttcaaa tttcttagat actctcaatt ggatgatcga
    62401 aatcactaca attaagaaga acaagattac ggcaatggca ttggcataac cgaattggtt
    62461 gtttttaaag gcatagttat aaaccaagag cccaagtgag gttgtggcat tgtttggacc
    62521 accaccggtc atggcaaaga cttggtcaaa ggcagtcagc ccacctttta gggctaggat
    62581 aaagaccata gagacacttg gtagcaagta aggcaattca atgttccaga aaacttgctt
    62641 gctcgtcgca ccatcaatcc ttgctgcctc tgtaatctca gttggaatag attgcaaacc
    62701 agctaggaag atgatgatgg gcatagccac cccttgccaa agaaggacaa agacagccgc
    62761 aaagattgct ccccacttag tccctaaaag actggtttgg agaaattcaa tatgaagggc
    62821 attcccaatc gctggaagac cgtagttgaa gacttgcttg aagatcaaag ccactgtcaa
    62881 accagataaa acagctggga agaagaacca agcacggaag aaggtttggc ctttgatttt
    62941 agaattcaag acacgcgcaa tgaagatccc gagtgcaatc tcaccaacca ccatggcaat
    63001 cgtaatgatt gcggtaaagc caatcgcatt catgaatttt ggatccatga agaggagctt
    63061 aaagttgttt aagccgacaa atttgtagtt ataagtcaat cctgtccagt tggtaaaact
    63121 gtaaaaggct ccttgaaaca tcggcacata gaagaaaatt gcttgtaaca agagggggat
    63181 gaccacaaaa gcccatgccc aatatttttg taatactttt ttcatagtct ctctactcct
    63241 aatccacatc cgctttcatc gggttaaaga aggcattcaa atcattgacc atgccttgtt
    63301 tatcaccggt caagacatag ttcatggtca aggtatggaa gtctgcttca ctggtccagt
    63361 attgttgcaa ccagaccaag tgacgatccg taaaggcata ttcggtcata ccagcaagcg
    63421 gtgaatcttc tcctgcttgt ttgacccctt cgatcgctgt tggagatccg tccacatcgt
    63481 agtatttttg catgacttct ggacgggtca tatattccac aaaggcattg gcttcttttg
    63541 gatgtttggt ggtggctgag atagaccatg ccaagtctcc cgcaccaacg gttaagcttt
    63601 gtcctttttc ttttcctgga atcatgaagg tcccaatctt aaagttcggt ttttgttcat
    63661 taatcgctgt gatcgcccaa gacccatttg gtgtcatgag gacatcccca cgtgcgaagg
    63721 ctccgataac atcggtatag ccagcacctt cccagttctt ttgcttagag ccattgatgc
    63781 gaaggatgtc catgaccttg atatcatctt tcataatcgg atccgacaat ttaatggcat
    63841 ttggttgaga ataacgaagg tattgatttg cttcttttcc tccacctgtt gctgtcgcaa
    63901 aggctaattg attgtaacca ttgagtgtcc aagcatctgc acctgcaatt ccaaatggtg
    63961 tttgtccttt agcaacgata tctttgacta actgttcaaa ttcatcccag gtttcaggaa
    64021 ccttcaagcc cagttcttcg aatttatctt tgttgtagta aattccataa gcattagctg
    64081 taaaaggaac gttgtaaact ttttcgttta cagcatattt ttcagcgtag ccatttttca
    64141 cgcgtttcag gtagtctttg ttgctcaaat cttcaaaaac acctgctttt gcccattctt
    64201 gcagttcgat ggactgtggg taaatattga ccacatcagg cacatctcct gcgagaacgc
    64261 gtgtcttcaa tacttcacca gcatttggta cattgacgac tttgaccttg atcttagggt
    64321 tttccttctc aaaatcacga gtgatttctt ccaaggtttt ggtcatttct tttttctggt
    64381 tgaaatactc gatggtcact gtgccatccg cagatttacc atagttggag caagcgccga
    64441 gcccaaccaa agctaaacct gtagttgcaa gaagtccgat ttttttatac cattccatta
    64501 gaaagcctcc tttataaatt tatacaccct tatcatagtc ttctaaaaat atactgtcta
    64561 ctcaaaaaat ctccttggga taagataaca gttaggcccg catacattag ttctgcacct
    64621 gagtaaattt cgccattttc ctgtaattta tatagtccct cttcatccaa atcttttaat
    64681 tttaaagttg tttccatggt ctctacaaca gataaaacgc gaacgtaggt tacaatcgtt
    64741 tgattttcgt aattaaattg tacagctgct tcattggata cagtatcagg attaattagt
    64801 ctatactgct gtcctaactg aactactggt cgtaattctt tatacaagtt cacctgatta
    64861 gcaatcgtag ctttctcttc atctgataaa tttgtcaaat caagttcata gcccaaattt
    64921 cccatcattg ctacaaggcc acgtgtttct aatggtgtca ttcgtcccat ctgatgattc
    64981 ggtactgctg acacatgagc ccccatagaa atggttggat agagatagga tgaaccgtat
    65041 tgaattggta aacgtgcaat ggcatcagta ttatcactag cccaaacttg tgggaaatag
    65101 cgcatcatac caagatcatt tcgtccacca ccaccagagc aggactcaaa gagaatatgg
    65161 ctgtgcttct ctgtcagata agaaacgagt tcgtaaagcc ccagcatgta ctgatgagat
    65221 tgcatctgtg tctctagata agttaatcca ttccctagct tagtgatatt gcggttcata
    65281 tcccatttaa tgtaatcaat atcatgataa gataggagtt gatctaagac acttttcaag
    65341 tattctacta cctgaggatt ggcaagatta agtactaatt gattccgaga ataagtatgc
    65401 tcatagtcag gaacctgaat agcccagtca ggatgtcgac gatacaaatc actatctaca
    65461 gaaatcattt cgggttctaa ccaaagtcca aactgcaaac ctctttcatg gatagctgaa
    65521 atcagacttt ctagacttcc acccagtttt tcctcattaa caacccaatc acctaaagca
    65581 cgattatcat caaaacgatt gccaaaccaa ccatcatcta atacaaaaag ttcaatgcca
    65641 actttcttag cttcatctgc taactctaac agtttttcta tctgaaagtc aaagtaagta
    65701 gcttcccagt tattgattag aattggacgt tcttttttag aaaattcact tagcataatg
    65761 tgcttcagta caaaattctg actttcatga ctaataccag ttaatccctg atctgaatgg
    65821 gtcactaaag ctaccggtgt ttcaaagtat tcctcaggag ctaacttaca agaaaagttt
    65881 tctggattaa tgccaatagc cacccgaact tcattcaatt gatttttttg aacaaaagct
    65941 tcaaagttgc cactatacat tagttgaata gcaaacacat tcccagcatc ctctgtgact
    66001 ccttgttcgc atagtaaaag agctggtgtt tgagcatgac cagaagcacc tcggtttgag
    66061 ctaatcgaaa agattccttg ttctacctgt tgacgtctaa cagtcttttc acgagcataa
    66121 gcaccctgca gagttactat ttcgtaatct gcagctggaa aatcagccat aaaagaaaaa
    66181 tctttatgga tgacaacttc ctgattacta ttattatcta atttactgta gctagcaata
    66241 gtcgcatcat tattaaaagt agtataatac aaagtcagac taagttgagc cttagaatct
    66301 tctaacatta agacaagagt ctctgtatcg tccatgctat gtggagaagg taagccctgt
    66361 ggaccattct gaccttttaa aatctttgct tctacaaatc gaaagtctgt tacttcagtt
    66421 acactatgct gaacctgtat ggttggtttc ctaaaatctc ctaagccatg ttgtccaaaa
    66481 atctgtcgct gagtatctaa actaaaggtt cgattagtag ccgttggatt tcctgaaaag
    66541 gcatggtctc gttcataaac actattggaa cctttatagt tcttaatagt ctttcctaaa
    66601 tgtttcaaaa gtaagtagcc atttcgattt tcaataatca aacttagatt tttactctca
    66661 acataaaata gattattctc tatcctaact cccatttact tcacctcatc actttattga
    66721 ttatatttta tcacctgaaa tcgctttcca aaatagaaaa atgtctcaag aatatggtaa
    66781 aatgttaggt aggaggtagc acatgctagt tttttcagaa taccagactg gaacaattga
    66841 ccttgcccta agcttttatg gatatgagga atgcacacct aattactctt ttggtccagc
    66901 cattcgtgat acatacgtcc tacattacat tactaaagga caaggaaaat ttcattacaa
    66961 gggtaaaatt gttgatttaa aagaaggaga tttctttcta ttaaaaccag aggaactaac
    67021 cttttatcaa gcagatagta aagaaccttg ggcctactac tggttaggaa tcactggagg
    67081 gaaatcccct gattattttg ctctttctca aatttctgat caatcctatc tcatccaatc
    67141 tgaaacttgt catacccaga ctactgcaaa actcatctca gacattgtcc gcttcgctca
    67201 gattacaaaa tcaagtgaat tatctcaact ccatatcatg ggacaacttc atgaactgat
    67261 gtttcatctg ggaactattg ctcccaatca gaaaaaaaag aatatttcat caacccacca
    67321 actctatctt gaatgcaaac gattaattga tagccactat cctcaatcac ttacaattca
    67381 agatttagca aaagaactat ccgttcacag aagctactta tcaagcgtat tcaaagaatt
    67441 taatacctta tcacccaaag aatacctact ctacattcga atgcaccgag ctagacaact
    67501 tctcgaaaat acccaagagt ccatcaaggt aattgcatac tcggtaggtt tttcagatcc
    67561 actccatttt tcgaaagctt ataaacaata ctttaatcag actccaagtc atacaagaaa
    67621 agaatactct caataccaac tagtgagaaa ggcaacatta tgaaatccta ccaagctgtc
    67681 taccaaatcc tatctaaaga aaccgactat atcagcggag aaaaaatcgc agaaaaacta
    67741 tccctaagcc gaacatcaat ttggaaagcc atcaagcgac tagaacaaga aggcattgaa
    67801 attgatagta tcaaaaatag aggatataaa ttgatgaatg gtgaccttat tcttccagag
    67861 attctagaag aaaatcttcc aattaaagtc agctttaaac ccgaaacaaa atcaacacaa
    67921 ctagatgcaa aagaagcaat tgatttaggc catgaagcaa ataccctcta tctagcttcc
    67981 tatcaaacag caggccgagg ccgttttcaa cgttccttct actcaccaca aggtggtatt
    68041 tatatgacac tccatcttaa accaaatctc ccctatgaca aattaccatc ctacacacta
    68101 cttgtagctg gagctgtcta caaagctatt aagaacctaa ctttaataga tgtcgacata
    68161 aaatgggtca atgatatcta tctaaacaat cataaaattg gaggaatcct tactgaagca
    68221 atgacctctg tagaaactgg cttagtcaca gatatcatta ttggagtagg tatcaatttc
    68281 actattaaag acttccctca ggaattaaaa gaaaaagctg ccagcttatt taaagctaca
    68341 gctcctataa caaggaatga attgatcata gaaatctggc gtgctttctt cgaaacacca
    68401 gcagaagagc tattatacct atacaaaaaa cagtcattca ttctaggaaa agaagtcact
    68461 ttcacactag agcaaaaaga ctacaaggga cttgctaaag acatctcaga aaatggaaaa
    68521 cttttaattc aatgtgataa cggaaaagaa atctggctaa atagtggcga aatttctctc
    68581 aatagttgga agtaaaataa cacaattata atataaacga tataaaaata acttcagatt
    68641 agtaattcaa ttaagtttta cggatctgaa gttttattgt ctctaaaaac aaaaaagaga
    68701 gttacaaact ctcattaaaa cggagaataa gggattcgaa cccttgcgcc agttacccga
    68761 cctaacgatt tagcaaaccg tcctcttcag cctcttgagt aattctccaa ttaatgggca
    68821 cgagtggact cgaaccaccg acctcacgct tatcaggcgt gcgctctaac cacctgagct
    68881 acgcgcccaa gttaaaaaac ttggtaattt gaacaaagtt caaagcgggt gacgagaatc
    68941 gaactcgcga caacagcttg gaaggctgta gttttaccac taaactacac ccgcataaat
    69001 actatcaata aaatggcgcg agacggaatc gaaccgccga cacatggagc ttcaatccat
    69061 tgctctacca actgagctac cgagccttat tgcgggagca ggatttgaac ctacgacctt
    69121 cgggttatga gcccgacgag ctaccgagct gctccatccc gcgttaataa tataaaagga
    69181 ggatgtggga ttcgaaccca cgcacgcttt tacacgcctg acggttttca agaccgttcc
    69241 cttcagccgg acttgggtaa tcctccaata ttcaaatgga ccttgtagga cttgaaccta
    69301 cgaccactcg gttatgagcc gagagctcta accagctgag ctaaaggtcc gacaagatca
    69361 ttatagcggc gaaggggatc gaacccccga cctcccgggt atgaaccgga cgctctagcc
    69421 agctgagcta caccgccatg aatcgggaag acaggattcg aacctgcgac accttggtcc
    69481 caaaccaagt actctaccaa gctgagctac ttcccgagtt aaatagaaaa atgcacccta
    69541 gaggagtcga acctctaacc gcctgattcg tagtcaggta ctctatccag ttgagctaag
    69601 ggtgctccat attatgccga ggaccggaat cgaaccggta cgatcgttac caatcgcagg
    69661 attttaagtc ctgtgcgtct gccagttccg ccaccccggc ctctctaagc gaacgacggg
    69721 attcgaaccc gcgaccccca ccttggcaag gtggtgttct accactgaac tacgttcgca
    69781 ctgttttctt ctatctaaaa atgccggcta catgacttga acacgcgacc ctctgattac
    69841 aaatcagatg ctctaccaac tgagctaagc cggctcattt gttatatctt aatgcgggtt
    69901 aagggacttg aacccccacg ccgttaagcg ccagatccta aatctggtgc gtctgccaat
    69961 tccgccaaac ccgcatatat gacccgtact gggctcgaac cagtgaccca ttgattaaaa
    70021 gtcaattgct ctaccaactg agctaacgag tctaaaataa cttgcgttac cttaaacggt
    70081 cccgacggga atcgaacccg cgatcttcgc cgtgacaggg cgacgtgata accgctacac
    70141 tacgggacct
//
LOCUS       DBERMR010000005        51141 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.005, whole genome shotgun sequence.
ACCESSION   DBERMR010000005 DBERMR010000000
VERSION     DBERMR010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 51141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51141
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.005"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            155..943
                     /gene="codY"
                     /locus_tag="ACL8A1_000373"
     CDS             155..943
                     /gene="codY"
                     /locus_tag="ACL8A1_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HGS1201201.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            943..1518
                     /locus_tag="ACL8A1_000374"
     CDS             943..1518
                     /locus_tag="ACL8A1_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HGS1201202.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            1600..1685
                     /locus_tag="ACL8A1_000375"
     tRNA            1600..1685
                     /locus_tag="ACL8A1_000375"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1634..1636,aa:Leu,seq:aag)
     gene            1968..3098
                     /locus_tag="ACL8A1_000376"
     CDS             1968..3098
                     /locus_tag="ACL8A1_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1201203.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(3245..3391)
                     /locus_tag="ACL8A1_000377"
     CDS             complement(3245..3391)
                     /locus_tag="ACL8A1_000377"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201204.1"
                     /translation="MEIKEQTRKLAVSCSKYIFEVADKTDEISNHTYGKATLTWFEEI
                     FEEY"
     gene            3599..4357
                     /locus_tag="ACL8A1_000378"
     CDS             3599..4357
                     /locus_tag="ACL8A1_000378"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGS1201205.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            4444..4956
                     /locus_tag="ACL8A1_000379"
     CDS             4444..4956
                     /locus_tag="ACL8A1_000379"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HGS1201206.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            5138..6082
                     /gene="metA"
                     /locus_tag="ACL8A1_000380"
     CDS             5138..6082
                     /gene="metA"
                     /locus_tag="ACL8A1_000380"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HGS1201207.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWINHAVYQETPFDWRKIEDDASAYGYL"
     gene            6091..6768
                     /locus_tag="ACL8A1_000381"
     CDS             6091..6768
                     /locus_tag="ACL8A1_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HGS1201208.1"
                     /translation="MTYLDAFKSGILVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            6867..7625
                     /gene="tpiA"
                     /locus_tag="ACL8A1_000382"
     CDS             6867..7625
                     /gene="tpiA"
                     /locus_tag="ACL8A1_000382"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HGS1201209.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            7663..9168
                     /gene="lytC"
                     /locus_tag="ACL8A1_000383"
     CDS             7663..9168
                     /gene="lytC"
                     /locus_tag="ACL8A1_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HGS1201210.1"
                     /translation="MSVTFFIGEERLKIKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSAEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMIQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            9665..10183
                     /locus_tag="ACL8A1_000384"
     CDS             9665..10183
                     /locus_tag="ACL8A1_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HGS1201211.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            10313..10819
                     /locus_tag="ACL8A1_000385"
     CDS             10313..10819
                     /locus_tag="ACL8A1_000385"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HGS1201212.1"
                     /translation="MTKKIVAIWAQDEEGVIGKDNRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            11021..12253
                     /gene="clpX"
                     /locus_tag="ACL8A1_000386"
     CDS             11021..12253
                     /gene="clpX"
                     /locus_tag="ACL8A1_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HGS1201213.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            12262..12849
                     /gene="yihA"
                     /locus_tag="ACL8A1_000387"
     CDS             12262..12849
                     /gene="yihA"
                     /locus_tag="ACL8A1_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HGS1201214.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            12860..13240
                     /locus_tag="ACL8A1_000388"
     CDS             12860..13240
                     /locus_tag="ACL8A1_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGS1201215.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            13292..14182
                     /gene="rapZ"
                     /locus_tag="ACL8A1_000389"
     CDS             13292..14182
                     /gene="rapZ"
                     /locus_tag="ACL8A1_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HGS1201216.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLVQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            14179..15156
                     /locus_tag="ACL8A1_000390"
     CDS             14179..15156
                     /locus_tag="ACL8A1_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HGS1201217.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDFRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            15153..16064
                     /gene="whiA"
                     /locus_tag="ACL8A1_000391"
     CDS             15153..16064
                     /gene="whiA"
                     /locus_tag="ACL8A1_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HGS1201218.1"
                     /translation="MSFTVAVKEEILGQHHLSWHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(16098..17066)
                     /locus_tag="ACL8A1_000392"
     CDS             complement(16098..17066)
                     /locus_tag="ACL8A1_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGS1201219.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPGQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            17202..18059
                     /gene="cdaA"
                     /locus_tag="ACL8A1_000393"
     CDS             17202..18059
                     /gene="cdaA"
                     /locus_tag="ACL8A1_000393"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HGS1201220.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERW
                     LGGWKHEKK"
     gene            18046..18825
                     /locus_tag="ACL8A1_000394"
     CDS             18046..18825
                     /locus_tag="ACL8A1_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HGS1201221.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSISARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVLVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            18849..20201
                     /gene="glmM"
                     /locus_tag="ACL8A1_000395"
     CDS             18849..20201
                     /gene="glmM"
                     /locus_tag="ACL8A1_000395"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HGS1201222.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            20275..20649
                     /locus_tag="ACL8A1_000396"
     CDS             20275..20649
                     /locus_tag="ACL8A1_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HGS1201223.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWESENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            20651..21499
                     /locus_tag="ACL8A1_000397"
     CDS             20651..21499
                     /locus_tag="ACL8A1_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HGS1201224.1"
                     /translation="MKLAVFTDSSAYLSEETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            21776..22543
                     /gene="dapB"
                     /locus_tag="ACL8A1_000398"
     CDS             21776..22543
                     /gene="dapB"
                     /locus_tag="ACL8A1_000398"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HGS1201225.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            22540..23739
                     /locus_tag="ACL8A1_000399"
     CDS             22540..23739
                     /locus_tag="ACL8A1_000399"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HGS1201226.1"
                     /translation="MRLTQMPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIH
                     DVDIATSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSA
                     VSFVRSLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDA
                     LRIMRGFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLA
                     SMIESQAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKS
                     WKTSRQFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNLQAI
                     TEKYQSLTIHDKKEIQINGDILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIH
                     AYLREKK"
     gene            23736..25607
                     /locus_tag="ACL8A1_000400"
     CDS             23736..25607
                     /locus_tag="ACL8A1_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HGS1201227.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELERAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQDVSFAYENKPILQD
                     FNLLVQVKDRIGIVGDNGVGKSTLLNLIAGSLESTKGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(25750..26934)
                     /gene="mntE"
                     /locus_tag="ACL8A1_000401"
     CDS             complement(25750..26934)
                     /gene="mntE"
                     /locus_tag="ACL8A1_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HGS1201228.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            27238..29934
                     /locus_tag="ACL8A1_000402"
     CDS             27238..29934
                     /locus_tag="ACL8A1_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HGS1201229.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LLEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEDHVLTGAELNELSDEDFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            29947..30738
                     /gene="yghU"
                     /locus_tag="ACL8A1_000403"
     CDS             29947..30738
                     /gene="yghU"
                     /locus_tag="ACL8A1_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HGS1201230.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTVGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTEDVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            30854..31264
                     /locus_tag="ACL8A1_000404"
     CDS             30854..31264
                     /locus_tag="ACL8A1_000404"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HGS1201231.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANHDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRV
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            31276..32871
                     /locus_tag="ACL8A1_000405"
     CDS             31276..32871
                     /locus_tag="ACL8A1_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201232.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPRSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTK
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESINGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKLR"
     gene            32938..33102
                     /locus_tag="ACL8A1_000406"
     CDS             32938..33102
                     /locus_tag="ACL8A1_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HGS1201233.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            33181..33909
                     /gene="yaaA"
                     /locus_tag="ACL8A1_000407"
     CDS             33181..33909
                     /gene="yaaA"
                     /locus_tag="ACL8A1_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HGS1201234.1"
                     /translation="MKILIPTAKEMNTDLPSIEAISLKPESQAVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTTQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(34186..34734)
                     /locus_tag="ACL8A1_000408"
     CDS             complement(34186..34734)
                     /locus_tag="ACL8A1_000408"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HGS1201235.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIK"
     gene            35055..35543
                     /locus_tag="ACL8A1_000409"
     CDS             35055..35543
                     /locus_tag="ACL8A1_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HGS1201236.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQVDQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKIIFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            35540..36727
                     /locus_tag="ACL8A1_000410"
     CDS             35540..36727
                     /locus_tag="ACL8A1_000410"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HGS1201237.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTAILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            36720..37085
                     /locus_tag="ACL8A1_000411"
     CDS             36720..37085
                     /locus_tag="ACL8A1_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGS1201238.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGLEAGGRDNGAPGYRSERLIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            37101..38447
                     /gene="asnS"
                     /locus_tag="ACL8A1_000412"
     CDS             37101..38447
                     /gene="asnS"
                     /locus_tag="ACL8A1_000412"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HGS1201239.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            38600..38890
                     /gene="rpsF"
                     /locus_tag="ACL8A1_000413"
     CDS             38600..38890
                     /gene="rpsF"
                     /locus_tag="ACL8A1_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HGS1201240.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            38902..39372
                     /gene="ssbA"
                     /locus_tag="ACL8A1_000414"
     CDS             38902..39372
                     /gene="ssbA"
                     /locus_tag="ACL8A1_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HGS1201241.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            39404..39643
                     /gene="rpsR"
                     /locus_tag="ACL8A1_000415"
     CDS             39404..39643
                     /gene="rpsR"
                     /locus_tag="ACL8A1_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HGS1201242.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            39938..41338
                     /locus_tag="ACL8A1_000416"
     CDS             39938..41338
                     /locus_tag="ACL8A1_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HGS1201243.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            41340..41699
                     /locus_tag="ACL8A1_000417"
     CDS             41340..41699
                     /locus_tag="ACL8A1_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HGS1201244.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            41753..42502
                     /locus_tag="ACL8A1_000418"
     CDS             41753..42502
                     /locus_tag="ACL8A1_000418"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HGS1201245.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVNPYSNLNESEHYLLARHEITTNLEEICRSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            42492..42791
                     /locus_tag="ACL8A1_000419"
     CDS             42492..42791
                     /locus_tag="ACL8A1_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HGS1201246.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            42862..43797
                     /locus_tag="ACL8A1_000420"
     CDS             42862..43797
                     /locus_tag="ACL8A1_000420"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HGS1201247.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            43883..44395
                     /locus_tag="ACL8A1_000421"
     CDS             43883..44395
                     /locus_tag="ACL8A1_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HGS1201248.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACKHLGAPYNASFYPDAPGFYCSQ
                     YIAEILPIFETIPMKFGDGEQAISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            44379..45028
                     /locus_tag="ACL8A1_000422"
                     /pseudo
     CDS             44379..45028
                     /locus_tag="ACL8A1_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614493.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
     gene            45129..45326
                     /locus_tag="ACL8A1_000423"
     CDS             45129..45326
                     /locus_tag="ACL8A1_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HGS1201249.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTKE"
     gene            complement(45369..46814)
                     /locus_tag="ACL8A1_000424"
     CDS             complement(45369..46814)
                     /locus_tag="ACL8A1_000424"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HGS1201250.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVVFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQSYLVRRVYGLTFDVGVFLNISPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            46920..48542
                     /locus_tag="ACL8A1_000425"
     CDS             46920..48542
                     /locus_tag="ACL8A1_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HGS1201251.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            48745..50703
                     /locus_tag="ACL8A1_000426"
     CDS             48745..50703
                     /locus_tag="ACL8A1_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS1201252.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKAVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            50875..>51141
                     /locus_tag="ACL8A1_000427"
     CDS             50875..>51141
                     /locus_tag="ACL8A1_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1201253.1"
                     /translation="MKLKLLRVDTKVIMGSFLLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKKIAKIRKKVIEGL"
BASE COUNT        15129 a         9482 c        11660 g        14870 t
ORIGIN      
        1 caacttgtca atagaaaagg aacaatttaa ttaaaaatat tctggttttt aattaaattg
       61 taagtcaatg caagtcaata aggaattttc agataattat tgaaaaagag acgtttttat
      121 gttataatga tagcgattaa attcgaggtg aaaaatggca catttattag aaaaaactag
      181 aaaaattaca tcaattttga aacgctcaga ggagcagttg caggatgagc tcccttacaa
      241 cgctattacg cgtcagttag cggatattat tcattgcaac gcctgcatta tcaatagtaa
      301 gggacgtctg cttggctatt ttatgcgtta taaaacaaat acagatcgcg tagagcaatt
      361 cttccaaact aagattttcc cagatgacta cgttcaaggg gctaatatga tttacgaaac
      421 agaagcaaac ttacctgttg agcatgatat gagtattttc cctgttgaga gtagagatga
      481 ctttccagat ggcttgacga ctattgcacc gattcatgta tcggggattc gccttggttc
      541 tttgattatt tggcgtaatg ataaaaaatt cgaagatgag gacttggttc ttgttgagat
      601 tgccagtacc gttgttggga ttcagcttct taacttccaa cgtgaagaag atgagaaaaa
      661 tattcgtcgt cgtactgctg tcaccatggc ggtcaatacc ctttcttact ccgaactccg
      721 tgctgtttca gcaattttag gggaattaaa tggaaatgaa gggaagttga ctgcgtcagt
      781 gattgcagat cgtatcggaa tcactcgctc tgtgattgtc aatgctcttc gtaaacttga
      841 gtctgcgggg attattgaaa gtcgctcact tggaatgaag ggaacctatc ttaaggtctt
      901 gatttcagat atttttgaag aagtgaagaa aagagattac taatgacaaa ggctttaatt
      961 tcgattgatt atacagaaga ttttgttgct gatagtggga aactaacagc aggtgctcca
     1021 gctcaggcga tttcggatgc catcagcaag gtaactcgat tagcttttga acgaggagat
     1081 tatatcttct ttactattga tgctcatgaa gaaaacgatt gtttccatcc ggaaagtaag
     1141 ttatttcctc ctcataatct gattgggacg agtggacgga atttatatgg agatttgggg
     1201 atcttttatc aagagcatgg ttcagacagt cgtgtctttt ggatggataa acgccattac
     1261 tcagcttttt cagggactga cctagatatt cgtttgagag agcgtagagt gtctaccgtt
     1321 atcttaacag gtgtcttgac ggatatctgt gtcctacata cagctataga tgcctataat
     1381 ctaggatatg acatcgagat tgttaaacca gctgttgctt ccatctggcc tgaaaatcat
     1441 caatttgccc taggtcattt caaaaataca ctcggagcta agttagtaga tgaaaatcta
     1501 aatgaacttt ctgagtaacc tggttggtga aaatgaaaaa atttgaaaaa aagtgttgac
     1561 aagcatccca aaagttgata tactagtaaa gtaatcgatg cggggatggc ggaattggca
     1621 gacgcgcagg actaaggatc ctgtgaccgc tttaggtcgt gagggttcaa gtccctctct
     1681 ccgcatagga aaagagttag ctaaagctag ctcttttgta tttctctaaa aaattagaat
     1741 ccagtcaaat gtagtggaga atcagcgaaa atatgggaaa actgatcctt gtttctgctt
     1801 ttgtcataga agaataataa agattatctt cttcaaattc tccgatattc tctaaagttt
     1861 tgtgcaagtt gcacagaact tttttatttt tttggtcatc ttgccataga aatataaagc
     1921 gttttcatct ataatataat tatcaaaaga caaaaggagt tcacctcatg gtagaattga
     1981 atcttaaaaa tatttacaaa aaatatccaa acagcgaaca ctattcagtt gaagatttca
     2041 acttgaacat caaagataaa gaatttatcg ttttcgtagg accttcagga tgtggtaaat
     2101 caactacact ccgtatgatt gctggtcttg aagacattac agaaggtact gcatctatcg
     2161 atggcgtggt tgtcaacgac gtagctccaa aagaccgtga tatcgccatg gtattccaaa
     2221 actacgctct ttacccacac atgactgttt atgacaacat ggctttcggt ttgaaattgc
     2281 gtaaatacag caaagaagac attaacaaac gtgttcaaga agcagctgaa atacttggat
     2341 tgaaagaatt cttggaacgt aaaccagctg acctttcagg tggtcaacgt caacgtgttg
     2401 ccatggggcg tgcgattgtc cgtgatgcga aagtattctt gatggacgaa cctttgtcaa
     2461 acttggatgc caaacttcgt gtatcaatgc gtgctgaaat cgctaaaatt caccgtcgta
     2521 tcggagctac aactatctat gtaactcacg accaaacaga agcgatgaca cttgcagacc
     2581 gtatcgttat tatgtcagct actaagaacc ctgctggtac aggtactatc ggacgtgtag
     2641 aacaaatcgg tactcctcaa gaagtttaca aaaatccagt taacaaattt gttgcaggat
     2701 tcatcggaag cccagctatg aatttcatca acgtgaaatt ggttggtagc gaaattgttt
     2761 ctgacggttt ccgtttgaaa gtgccagaag gagcattgaa agttcttcgt gaaaaaggct
     2821 acgaaggaaa agaattgatc tttggtatcc gtccagaaga cgtgaatgca gaacctgctt
     2881 tccttgaaac attcccagac tgtgttgtaa aagcgactat ctctgtatca gaactgcttg
     2941 gttcagaatc tcacctttac tgccaagttg gtaaagacga gtttgttgca aaagttgatg
     3001 ctcgtgacta cttgcaaaca ggtgcaacag ttgagcttgg atttgacttg aacaaagcac
     3061 acttcttcga tgtagaaact gaaaaaacaa tctactaaaa taaataaaat tcaaagcact
     3121 acaagaaaag atatctcttt atcaattgta gtggagagat atcagttaat ctagggagag
     3181 aaacaaaatg cttctctcct ttttgctagg gaagtcatat tatgcatcta tattgtgatg
     3241 ctctttaata ctcttcgaaa atctcttcaa accacgtcaa cgtcgccttg ccgtacgtat
     3301 gattactgat ttcgtcagtt ttatctgcaa cctcaaagat gtactttgag cagcttacgg
     3361 ctagtttcct agtttgctct ttgatttcca ttgagtatta tttgtgggta ccatctacaa
     3421 gtgaagctat atgcgtaaac tacgtgagca attgaattcg aactagagag gtaataataa
     3481 atttatgcta tagttatggt gacttgtatg cttttgattc tagtttatca aataatagat
     3541 tagaattgtc agataatatc attttgtgtt ataatgaaga aaaaacagag gtgttcaaat
     3601 gtcagaagca ggtcataagt ttttagcaaa attggggaaa aaacgcttac gtccaggtgg
     3661 aaagcgtgcc acagattggt taattgcaga aggaggattt tcaaaagaaa agagaatact
     3721 agaggttgcg tgtaataggg gaactacagc aattgagttg gcacagcgtt ttggttgcaa
     3781 gataactgct gttgatatgg atgctcaagc tttagaagtg gctaaaaaat ctgctggaac
     3841 ggcaggtgtt gctcatttaa tcagttttga aagagcaaat gcaatgaaac ttccttatca
     3901 agatgctagt tttgatattg ttataaatga agctatgctg actatgcaag ccgatcaagc
     3961 taagaaaaaa tgtgtaatgg aatatctaag ggtattaaaa cctggaggtc ttctcttgac
     4021 acatgatgtg cttcttaagg aagctaaaga gtctatcaga caggaattat cacaagcaat
     4081 tcatgtaaat gtaggtcctt taactcaaga tggttgggaa caggtgatga tagaatcagg
     4141 ttattgtgat gtgaaagcat tgactggtga aatgacatta atgaaattat cgggtatgat
     4201 ttatgacgaa ggtttgctag gaactttgaa aatttgtgta aatgcttgta aaaaggagaa
     4261 tagaaagcag tttttaacta tgtataaaat gtttgctaag aataaacaga aattgggctt
     4321 tattgcgatg gctagttata aatcgtcaaa acgttagata attattgaag ttaacttttc
     4381 cttttttctt tcttaaaaaa tatgctataa tagagagtaa aaaactttga aagaaagaaa
     4441 aagatgaatt taaaagatta cattgcaaca attgaaaatt atccaaagga aggcattacc
     4501 ttccgtgata ttagtccttt gatggctgat ggaaatgctt atagctacgc tgttcgtgaa
     4561 atcgttcagt atgctactga caagaaagtc gacatgatcg tgggacctga agctcgtgga
     4621 tttatcgtgg gttgtccagt tgcctttgag ttgggaattg gttttgcgcc tgttcgtaag
     4681 ccaggtaaat tgccacgcga agttatttct gctgactatg aaaaagagta cggtgtcgat
     4741 actttgacta tgcacgcgga tgccattaag ccaggtcaac gtgttcttat tgtagatgac
     4801 cttttggcga caggtggaac tgttaaggca actatcgaga tgattgaaaa acttggtggt
     4861 gttatggcag gttgtgcctt ccttgttgaa ttggatgaat tgaacggccg tgaaaaaatc
     4921 ggtgactacg actacaaagt tcttatgcat tattaatgaa aacagtccct agggctgttt
     4981 tctctacact aggatataaa aatagactat aactagttag agaaaaacta taattgaaaa
     5041 ctatatcttc ttacagtata ataaaaggac taagtgtttg agatttgtct tcaaacatat
     5101 gcaattattc ctgaaagagt acagttagga gagggttatg ccgattcgaa ttgataaaaa
     5161 attgccagct gttgaaattt tacggacaga gaatatcttt gtcatggatg atcaacgtgc
     5221 tgcccaccaa gatatccgtc ctttgaagat tttaatttta aatctcatgc cacagaaaat
     5281 ggtcacagag acccagttgt tgcgccactt ggctaataca cccctacaac tggatattga
     5341 ttttctctat atggagagcc accgttctaa aacaactcgt tcagagcaca tggagacctt
     5401 ctataaaact tttcctgaag tcaaggatga gtattttgat gggatgatca tcacgggtgc
     5461 tccagttgag catttaccat ttgaggaagt ggactattgg gaggaattta gacagatgct
     5521 tgagtggtct aagactcatg tctattcgac ccttcatatc tgttgggggg ctcaggctgg
     5581 gctttatctg cgctatggtg tagaaaaata ccagatggac agtaagctat caggtattta
     5641 tcctcaggac accctaaaag agggtcacct tctatttaga ggctttgatg atagctatgt
     5701 atcccctcat tcacggcaca cggagatttc taaggaagag gtcttaaaca agaccaatct
     5761 cgagatttta tcagaaggac ctcaggttgg ggtttctatt ttggccagtc gtgatttacg
     5821 agaaatttat agttttggtc atttggagta tgaccgtgat accttggcaa aagagtattt
     5881 tcgagatcgt gatgcaggtt tcgacccaca tattccagaa aattacttta aagatgatga
     5941 tgttaatcag gtaccttgtc tttgttggtc ttcatctgca gccctctttt tcagtaattg
     6001 gataaaccat gcggtctatc aggagacgcc ttttgattgg agaaagatag aagatgatgc
     6061 atctgcatat gggtatttat aagaggaatt atgacatatt tagacgcttt taaatcaggt
     6121 atcttggttt taccgagtgc cctgctcttg cattttaagg aactctttcc ttctagcgac
     6181 gattttctgg tttggcaatt tttctatttg caaaatacga caggcttaga agaaatgtcg
     6241 ccaagccaga ttgctgaaag gattggcaag gaaatttcgg atgtcaacca gtccatttct
     6301 aatctgacgg aaaggggact gctccagtat cgtactatcg aattaaatgg cgaaattgaa
     6361 ttgctctttg atgctagttt ggccttggaa cgtttggatg acctgtttgg agcagttcat
     6421 tcaagttcag accagctgac acctcaaaac cagctcaagg atttggtgga aaccttccag
     6481 caggagttgg gacgattgtt gacgcctttt gagattgagg atttgaccaa gacactaaag
     6541 gaagatggaa ccagtgctga cttgattaag gaggctcttc gtgaagctgt tttgaatgga
     6601 aaaccaaact ggaagtacat tcaggcgatt ttgagaaact ggcgccatga aggaatcaag
     6661 agtgtggctc aaattgaggc caagagagca gaaagagaag caagcaatcc tcagttgaca
     6721 caggtatctg cagatttcag aaatgccatg gatctctgga aggattaatc catgcaagca
     6781 ggcttgaaat ccgagtaaga tttgcaagct gtgtataatt gtgatagaat aaaatagaaa
     6841 ataaattgaa aaaagaggta tgtgaaatgt cacgtaaacc atttatcgct ggtaactgga
     6901 aaatgaacaa aaatccagaa gaagctaaag cattcgttga agcagttgca tcaaaacttc
     6961 cttcatcaga tcttgttgaa gcaggtatcg ctgctccagc tcttgatttg acaactgttc
     7021 ttgctgttgc aaaaggctca aaccttaaag ttgctgctca aaactgctac tttgaaaatg
     7081 caggtgcttt cactggtgaa actagcccac aagttttgaa agaaatcggt actgactacg
     7141 ttgttatcgg tcactcagaa cgccgtgact acttccatga aactgatgaa gatatcaaca
     7201 aaaaagcaaa agcaatcttt gcgaacggta tgcttccaat catctgttgt ggtgaatcac
     7261 ttgaaactta cgaagctggt aaagctgctg aattcgtagg tgctcaagta tctgctgcat
     7321 tggctggatt gactgctgaa caagttgctg cctcagttat cgcttatgag ccaatctggg
     7381 ctatcggtac tggtaaatca gcttcacaag acgatgcaca aaaaatgtgt aaagttgttc
     7441 gtgacgttgt agctgctgac tttggtcaag aagtcgcaga caaagttcgt gttcaatacg
     7501 gtggttctgt taaacctgaa aatgttgctt catacatggc ttgcccagac gttgacggtg
     7561 cccttgtagg tggtgcgtca cttgaagctg aaagcttctt ggctttgctt gactttgtaa
     7621 aataatcagt aagtagcaaa agctaggtgg aacagcattc agatgtctgt tacatttttt
     7681 ataggagagg aaagattgaa aataaaaatt ggattagcaa gtatctgttt actaggcttg
     7741 gcaactagtc atgtcgctgc aaatgaaact gaagtagcaa aaacttcgca ggatacaacg
     7801 acagcttcaa gtagttcaga gcaaaatcag tcttctaata aaacgcaaac gagcgcagaa
     7861 gtacagacta atgctgctgc ccactgggat ggggattatt atgtaaagga tgatggttct
     7921 aaagctcaaa gtgaatggat ttttgacaac tactataagg cttggtttta tattaattca
     7981 gatggtcgtt attcgcagaa tgaatggcat ggaaattact acctgaaatc aggtggatat
     8041 atggcccaaa acgagtggat ctatgacagt aattacaaga gttggtttta tctcaagtca
     8101 gatggggctt atgctcatca agaatggcaa ttgattggaa ataagtggta ctacttcaag
     8161 aagtggggtt acatggctaa aagccaatgg caaggaagtt atttcttgaa tggtcaagga
     8221 gctatgatac aaaatgaatg gctctatgat ccagcttatt ctgcttattt ttatctaaaa
     8281 tccgatggaa cttatgctaa ccaagagtgg caaaaagtgg gcggcaaatg gtactatttc
     8341 aagaagtggg gctatatggc tcggaatgag tggcaaggca actactattt gactggaagt
     8401 ggtgccatgg cgactgacga agtgattatg gatggtgctc gctatatctt tgcggcctct
     8461 ggtgagctca aagaaaagaa agatttgaat gtcggctggg ttcacagaga tggtaaacgc
     8521 tatttcttta ataatagaga agaacaagtg ggaaccgaac atgctaagaa aatcattgat
     8581 attagtgagc acaatggtcg tatcaatgat tggaaaaagg ttattgatga gaacgaggtg
     8641 gatggtgtca ttgttcgtct aggttatagc ggtaaagaag acaaggaatt ggcgcataac
     8701 attaaggagt taaaccgtct gggaattcct tatggtgtct atctctatac ctatgctgaa
     8761 aatgagaccg atgctgagaa tgacgctaaa cagaccattg aacttataaa gaaatacaat
     8821 atgaacctgt cttaccctat ctattatgat gttgagaact gggaatatgt aaataagagc
     8881 aagagagctc caagtgatac agacacttgg gttaaaatca tcaacaagta catggacacg
     8941 atgaagcagg cgggttatca aaatgtgtat gtctatagct atcgtagttt attacagacg
     9001 cgtttaaaac acccagatat tttaaaacat gtaaactggg tagcggccta tacgaatgct
     9061 ttagaatggg aaaaccctta ttattcagga gaaaaaggtt ggcaatatac ctcttctgaa
     9121 tacatgaaag gaatccaagg gcgcgtagat gtcagcgttt ggtattaagc gatgatttga
     9181 aagagggatg tgatagtagc accctctttt tctttgtttt atgatagttc atcctcgagt
     9241 aaattcaaga tcttgctcgg aaatgaagct tatatagtag attgaatata gataaagacc
     9301 ttgtgattgg taaaacattt tagaaattca tttacctttc ctaatcgact tggtttcatc
     9361 ttatttcaat ctattatagt attggggaat ttcttcaaac cacatcagct tggtcagtcc
     9421 tacctgcgac ctcaaaactt gtgctttggt caagctgggt ttagtttcct agtttgctga
     9481 tggatttcca ttgactataa gcatccaacc ctcttttttg tcttctaaag aattcttaaa
     9541 ttatcagtct attgcaactt ttctcatata agttctttgt cttgctattg gttttcctta
     9601 gtagtatact aaggtagtaa tcattaagaa gtggttacaa aaaataatga atgaggtaaa
     9661 gaaaatggta gaattgaaaa aagaagcagt aaaagacgta acatcattga caaaagcagc
     9721 gccagtagca ttggcaaaaa caaaggaagt cttgaaccaa gctgttgctg atttgtatgt
     9781 agctcacgtt gctttgcacc aagtgcactg gtatatgcat ggtcgtggtt tccttgtatg
     9841 gcatccaaaa atggatgagt acatggaagc tcttgacggt caattggatg aaatcagcga
     9901 acgcttgatt acactcggtg gaagcccatt ctctacattg acagagttcc ttcaaaatag
     9961 tgaaatcgaa gaagaagctg gtgaataccg taatgttgaa gaaagcttgg aacgtgttct
    10021 tgttatctac cgttacttgt cagaactttt ccaaaaaggt ttggatgtca ctgatgaaga
    10081 aggtgacgat gtgacaaacg gtatctttgc aggcgctaaa actgaaacag ataaaacaat
    10141 ttggatgctt gcagccgaac ttggacaagc acctggtttg taagaaataa ggaaacatag
    10201 ataaatctgt aggaaatttc ttacagattt tttctatttt gtaagctatt ccaaaccagt
    10261 ctttttttga gttttttgat aaaatagtac tatcagtgaa aaggatggaa gcatgactaa
    10321 gaaaatcgta gctatttggg cccaggatga agagggtgtg attggtaagg acaatcgtct
    10381 gccttggcat ttgccagcag aattgcagca ctttaaagaa acaactctga atcatgctat
    10441 cttgatgggg cgtgtgacct ttgatgggat ggggcgtcgc ttgcttccaa aacgggaaac
    10501 cctgattttg acgcgtaatc cggaagaaaa gatagatggg gttgctactt ttcaggacgt
    10561 ccagtctgtt cttgactggt atcaggctca agaaaagaat ctctacatta tcggtgggaa
    10621 gcaaattttt caggcttttg aaccttacct tgatgaagtg attgtcactc acattcatgc
    10681 tcgggtggaa ggagatacct atttccctga agaacttgac ttgtctcttt ttgagacagt
    10741 ttcaagcaaa ttttacgcca aagatgagaa gaatccttat gattttacca tccaataccg
    10801 caagagaaag gaagtctaat ggaacggagt ttatttggtc tattcacggc ttttctgtgt
    10861 ttcatctgct ttctggcagg agcacaagct tttcgtaaaa agcgttacgg gctttctatc
    10921 ctcctttggt taaatgcctt taccaatctg gtcaatagta ttcatgcttt ttacatgacc
    10981 ttattttaga tagaatgaca gtatagaatg gaaggaaatc atgtctacaa atagaaaaaa
    11041 tgatatgatg gtttattgct cattttgtgg caaaaaccaa gaagaagtac aaaaaataat
    11101 tgctggcaac aatgctttta tttgtaatga atgcgtggag ttagctcagg aaatcattcg
    11161 agaagaattg gttgaggaag tcttggcaga cttgtctgag gtgccaaaac caattgaact
    11221 cctccatatc ttgaaccact atgtaattgg ccaagatcgt gccaagcgtg ccttggcagt
    11281 ggcggtttat aaccactaca aacgcatcaa tttccacgat acacgcgaag agtcagaaga
    11341 tgtggatttg cagaagtcaa acattttgat gattggccca actggttcag ggaaaacttt
    11401 ccttgcccag accttggcta agagcttgaa tgtacctttt gctattgcgg atgcgacagc
    11461 tctgacggag gctggttatg tgggtgagga cgtggaaaat atcctcctca aactcttgca
    11521 ggttgctgac tttaacatcg aacgtgcaga gcgtggcatt atctatgtgg atgaaattga
    11581 caagattgcc aagaagagtg agaatgtgtc tatcacacgt gatgtttctg gtgaaggggt
    11641 gcaacaagcc cttctcaaga ttatcgaggg aactgttgct agcgtgccgc ctcaaggtgg
    11701 acgcaaacat ccacaacaag agatgattca agtggataca aaaaatatcc tcttcatcgt
    11761 gggtggtgct tttgatggta ttgaagaaat tgtcaaacaa cgtctgggtg aaaaagtcat
    11821 cggatttggt caaaataata aggcgattga cgaaaacagc tcatatatgc aagaaatcat
    11881 cgctgaagac attcaaaaat ttggtattat ccctgagttg attggacgct tgcctgtttt
    11941 tgcggctctt gagcaattga ccgttgatga cttggttcgc atcttgaaag agccaagaaa
    12001 tgccttggtg aaacaatacc aaactttgct ttcttatgat gatgttgagt tggaatttga
    12061 cgacgaagcc cttcaagaga ttgctaataa agcaatcgaa cggaagacag gggcgcgtgg
    12121 acttcgctcc attatcgaag aaaccatgct agatgttatg tttgaggtgc cgagtcagga
    12181 aaatgtgaaa ttggttcgca tcactaaaga aactgtcgat ggaacggata aaccgatcct
    12241 agaaacagcc tagaggtgac tatggaactt aatacacaca atgctgaaat cttgctcagt
    12301 gcagctaata agtcccacta tccgcaggat gaactgccag agattgccct agcagggcgt
    12361 tcaaatgttg gtaaatccag ctttatcaac actatgttga accgtaagaa tctcgcccgt
    12421 acatcaggaa aacctggtaa aacccagctc ctgaactttt ttaacattga tgacaagatg
    12481 cgctttgtgg atgtgcctgg ttatggctat gctcgtgttt ctaaaaagga acgtgaaaag
    12541 tgggggtgca tgattgagga gtacttaacg actcgggaaa atctccgtgc ggttgtcagt
    12601 ctagttgacc ttcgtcatga cccgtcagca gatgatgtgc agatgtacga atttctcaag
    12661 tattatgaga ttccagtcat cattgtggcg accaaggcgg acaagattcc tcgtggtaaa
    12721 tggaacaagc atgaatcagc aatcaaaaag aaattaaact ttgacccgag tgacgatttc
    12781 atcctctttt catctgtcag caaggcaggg atggatgagg cttgggatgc aatcttagaa
    12841 aaattgtgag gaaaagaaaa tggcaaaaac aattcataca gataaggccc caaaggctat
    12901 cggaccctat gttcaaggaa aaatcgttgg caaccttttg tttgctagcg gtcaagttcc
    12961 cctatcccct gaaactgggg aaattgtagg agagaatatc caagaacaga cagagcaagt
    13021 cttgaaaaac atcggtgcta ttttggcaga agcaggaaca gactttgacc atgttgtcaa
    13081 aacaacttgt ttcttgagcg atatgaacga ctttgttcct tttaatgagg tttaccaaac
    13141 ggccttcaaa gaggaattcc cagctcgttc agctgtggag gtagctcgtc ttcctcgtga
    13201 tgtaaaagtc gaaattgaag tcatcgcaga gattggataa gctagttgaa gtttggtgtt
    13261 gccaaacttc ttttgatata aggagaaaaa gatgacaaag aaacaacttc acttggtgat
    13321 tgtgacaggg atgagtggcg cagggaaaac tgtagccatt cagtccttcg aggatctagg
    13381 ttatttcacc attgataata tgccgccagc tctcttgcct aagtttttgc agctggttga
    13441 aattaaggaa gacaatccta agttggcctt ggtagtggat atgcgtagcc gttctttctt
    13501 ttcagagatt caagctgttt tggatgagtt ggaaaatcaa gatggtttgg atttcaaaat
    13561 cctctttttg gatgcggctg ataaggaatt ggtcgctcgt tacaaggaaa ccagacggag
    13621 tcacccacta gcagcagacg gtcgtatttt agatggaatc aagttggaac gtgaactctt
    13681 ggcacctttg aaaaatatga gccaaaatgt ggtggatacg actgaactca ctccacgtga
    13741 gctgcgcaaa acccttgcag agcagttttc agaccaagaa caagcccagt ctttccgtat
    13801 cgaagtcatg tctttcggat ttaagtatgg aatcccgatt gatgcggact tggtctttga
    13861 tgtccgtttc ttgccaaatc cctattattt accagaactg agaaaccaaa cgggtgtgga
    13921 tgaacctgtt tatgattatg tcatgaacca tcctgagtca gaagactttt atcaacattt
    13981 attggccttg attgagccga ttctgccaag ttaccaaaag gaaggtaagt ccgttttgac
    14041 cattgccatg ggatgtacgg gtggacaaca ccgtagtgtg gcatttgcta aacgcttggt
    14101 gcaggactta tccaagaatt ggtctgttaa tgaagggcat cgcgacaaag accgcagaaa
    14161 ggaaacggta aaccgttcat gagaaaacca aagataacgg tgattggtgg agggactgga
    14221 attcccgtca ttctaaaaag tctgcgggaa aaagatgtgg aaatcgcagc tatcgtgacg
    14281 gtggcagatg atggtggttc ttcaggtgaa ctccgaaaaa atatgcaaca gttgacaccg
    14341 ccaggtgatt ttcgtaatgt ccttgtggcc atgtcggata tgcctaagtt ttatgagaag
    14401 gtctttcagt atcggttctc tgaggatgcc ggagcctttg ctggccatcc attgggaaat
    14461 ctcatcattg ctggcttgtc agaaatgcag ggttcaacct ataatgccat gcagttattg
    14521 agcaaatttt tccatacaac agggaaaatt tatccttcca gtgaccatcc tttgaccctt
    14581 catgcagtct ttcaggatgg gacagaagtg gctggagaga gtcatattgt agaccatcga
    14641 ggcataattg acaatgtcta tgtgaccaat gccctaaacg atgatacgcc tctggccagc
    14701 cgtcgagtag tgcagaccat ccttgaaagt gacatgattg tcctagggcc aggttctctc
    14761 tttacctcta ttttgcccaa tatcgtgatt aaggaaattg ggcgggctct tttggaaacc
    14821 aaggcagaaa ttgcctatgt ctgcaatatc atgacccaac gtggggagac ggaacacttt
    14881 acagatagcg accacgtgga agtcttgcat cgtcaccttg gtcgcccttt tatcgacact
    14941 gtcttggtga atattgaaaa agtgcctcag gaatacatga attccaaccg ttttgatgaa
    15001 tacttggtgc aagtggaaca cgattttgta ggtctttgta agcaagtttc gcgcgtgatt
    15061 tcatctaact tccttcgtct ggaaaatggc ggtgccttcc acgatggaga tttgattgtg
    15121 gacgagttga tgcgcattat acaggtgaaa aaatgagttt cacagtagca gtaaaagaag
    15181 aaatcctggg ccaacaccat ctgagctggc atgaattatc tgccattatc aaaatgtctg
    15241 gtagcatcgg tctctcgact tcgggcttga ctttgtcagt tgtgacagaa aatgccaaac
    15301 tggcccgtca cctctatgag tcctttcttc atttctatga aatcaaatct gaaattcgtc
    15361 accaccaacg tagcaatctt cgcaagaatc gcgtctatac cgtttttaca gatgaaaagg
    15421 tgcaggattt gttaagtgat ttgcacttgg cagactcttt ctttggcctg gaaacaggta
    15481 ttgatgaggc gattttatcg gatgaggaag caggtcgtgc ctatctctgt ggcgctttct
    15541 tggcaaatgg gagcattcgt gaccctgagt caggcaagta ccagttggaa atcagttctg
    15601 tttatctgga ccacgcgcaa ggaattgcct cccttctcca acagttttta ctggatgcca
    15661 aggtgcttga gcgcaagaag ggggctgtga cctatctcca gagagcagaa gacattatgg
    15721 acttcttgat agtaatcgga gccatgcagg cacgtgatga ttttgagcgg gttaagattt
    15781 tgcgagaaac ccgtaacgac ctcaatcggg ctaataatgc cgagacagct aatatcgctc
    15841 ggacagtttc tgccagcatg aagaccatta acaatatcag taaaatcaaa gatatcatgg
    15901 gcttagaaaa tttgccagta gatttgcagg aagtggcgca actgaggatt cagcacccag
    15961 actactctat ccagcagtta gcagatagtc tcagcacccc tctgaccaaa agtggtgtca
    16021 atcacagact cagaaaaatc aataagatag cggatgaatt ataaaaccac gaatcctatg
    16081 tgactcgtgg ttctttttta taaactagta gagtgttttg gttgtacttt ttgttcaggg
    16141 tcaatgtagt tgatagcgtt gttgacagca gttggagctt ctccgaggcc tgtcgcaatc
    16201 agatcaattt ttccgtcata gtagcagcag tcaccgatag catagatacc tgcttggctg
    16261 gattcctgtt tgctgttgac gataatcttg tgacggttga ggtcgagccc ccagttttta
    16321 aggttaccga cagaagattt gaaaccatag ttgacaaaga ggtggtctag gtcaatggtt
    16381 tcagtttcat cagatttgac ttttgtgatt tcaagtttat caagtgtttt tccatttcca
    16441 aggagttggc caggggcgaa tggtgtcttg atggttacag atgattcttg caaggcttga
    16501 acactgtgtt ccaaggcacg gaaattatct ctgcggtgaa caagggtagt tggtgcgatt
    16561 ttttcaaaag ccaaagccca atccacagcc gagtctcccc caccaagaat cgtcactttc
    16621 ttaccagcgt attgctgaat gttagaaacg tggtagtgga tattttcata gccctcaacc
    16681 ccttcaagtt ccagcggacg tggtttgaag gcaccgccac ccatagcgat gataactgtt
    16741 ttagtcaggt gacttccttt agaagttgtg atggcaaatt cttcttcttg tttgtcaatc
    16801 tcaagaaccg tttcattgag atgaataggg gtatcaaatc catttagctg ttcaatcaag
    16861 cggttagtca actcttctcc agtcaggttt gggaagcctg gtacgtctag gatttccttt
    16921 tcagggtaga gaatagcagg ttgtccacct agctggggaa gagagtcgat gatttgaacc
    16981 ttggcttggc gtaggtgggc ataaaaggct gcaaaaagcc cgacaggacc accacccaca
    17041 atggtaatat catagagttg agacatagtt tctcctttgt tttttctagt cagtttattt
    17101 tatcatattt ttgttcaatg taaaatggac taggataggg aaaaaaatgc ccgaaaatgg
    17161 tataatagga cgaaagtgtt ttgataggga ggatatcagg gatgaatttt caacaattat
    17221 ccaatctgca atattggacc agtttgtttg caagtccatg gacgatagct atcaatctga
    17281 ttgatatttt gattgttgct tatattttat accattttac aaaagctatt gcaggaacca
    17341 agattatgat tttggtacgt ggagttttgg tatttatttt agctcaaatc cttgcaaata
    17401 tgattggttt gactacgatt tcttggttaa tcaatcaaat tattacttat ggggttattg
    17461 cggcggttgt tatcttctct ccagagattc ggactggttt ggaacgtttg ggaagagcga
    17521 cagatttctt ttccaatgcc cctattagtg ctgaggaaca gatgattcgt gcctttgtta
    17581 agtctgttga atacatgagt cctcgtaaaa tcggggcctt ggttgctatt caacgtgtac
    17641 gtaccttgca ggagtatatt tcgacaggaa tccccttgga tgctaagatt tctgcagaac
    17701 ttctcattaa catttttatt cccaacactc ccctacatga cggtgcggtg attatcaaag
    17761 aagaacgtat cgctgtgacg tctgcctatc tgcccttgac aaaaaacaca ggtatttcca
    17821 aggaatttgg gaccagacac cgggcggcta tcggtttatc agaagtctca gatgccttga
    17881 cttttgtcgt atcagaggaa acgggaggaa tttcgataac ctataatgga aggtttaagc
    17941 acaacctaac acttgatgaa tttgaaacag aattacgtga aatcttactt ccaaaagagg
    18001 aagtgggtct tagttttaaa gaacgatggc taggaggatg gaaacatgaa aaaaaatagt
    18061 ttatatatca tatcctcact cttttttgct tgtgtcttat ttgtctatgc tacggcgacg
    18121 aattttcaaa acagtatcag tgctaggcag gtaaaaacgg aaacctatac taatacagta
    18181 acaaatgtcc ctattgacat acgctataat agtgataagt attttattag cggttttgct
    18241 tcagaagtat tagtggtctt gactggtgca aatcgcctat cgctagctag tgaaatgcaa
    18301 gaaagtacac gtaaattcaa ggttactgct gacctaacag atgccggtgt tggaacgatt
    18361 gaagttcctt tgagcattga agatttaccc aatgggctga ccgctgtggc gactccgcaa
    18421 aaaattacag tcaagattgg taagaaggct cagaaggata aggtaaagat tgtaccagag
    18481 attgacccta gtcaaattga tagtcgggta caaattgaaa atgtcatggt gtcagataaa
    18541 gaagtgtcga ttacgagtga ccaagagaca ttggatagaa ttgataagat tatcgctgtc
    18601 ttgccaacta gcgaacgtat aacaggtaat tacagtggtt cagtaccttt gcaggcaatc
    18661 gaccgcaatg gtgttgtctt accggcagtt atcactccgt ttgatacaat aatgaaggtg
    18721 actacaaaac cagtagcacc aagttcaagc acatcaaatt caagtacaag cagttcatcg
    18781 gagacatctt cgtcaacgaa agcaactagt tcaaaaacga attaaaaata gaaaaggatt
    18841 ttataacaat gggtaaatat tttgggactg atggagtccg tggagaagct aacctagaac
    18901 taacaccaga attagccttt aaactaggac gttttggagg ctatgttctt agtcaacatg
    18961 aaacggaagc gccgaaagtc tttgtaggac gtgacacacg tatttcaggg gaaatgctgg
    19021 aatcggcctt ggtggcaggt ctcctttcag tagggattca cgtatacaaa cttggtgtcc
    19081 ttgcaacacc agcagtagct tacttggttg aaactgaagg agcaagtgcc ggtgtcatga
    19141 tttctgctag ccacaaccca gcccttgata acggaatcaa gttctttggc ggtgatggct
    19201 tcaaactaga tgatgaaaaa gaagcagaaa ttgaagcctt gctagatgct gaggaagaca
    19261 ctcttcctcg tccaagtgca gaaggcttag gaattttggt agattatcca gaaggcttgc
    19321 gtaagtatga aggatacctt gtttcaactg gaactcctct tgatggaatg aaggttgcct
    19381 tggatacagc taatggagca gcttctacca gtgcccgtca aatctttgca gaccttggtg
    19441 cccaattgac ggttatcggg gaaacaccag acggtcttaa catcaacctt aatgttggtt
    19501 caacacatcc agaagccctt caagaagtgg tcaaagaaag tgggtcagct attggtttgg
    19561 cctttgatgg agacagtgac cgcttgattg ctgttgatga gaatggtgac atcgtcgatg
    19621 gtgacaagat tatgtacatc atcggaaaat acctttctga aaaaggacaa ttggctcaaa
    19681 atacaattgt gacaactgtt atgtctaacc ttggtttcca caaggccttg aatcgcgaag
    19741 gtattaacaa ggcagttact gcagttggtg accgctacgt tgttgaagaa atgagaaaat
    19801 caggttacaa ccttggtggt gaacagtctg gtcacgttat cttgatggat tacaatacca
    19861 caggtgatgg tcaattatca gcagttcaat tgactaaaat catgaaggaa actggtaaga
    19921 gcttatcaga gttggcggca gaagtaacga tttatccaca aaaattagtt aatatccgag
    19981 tggaaaacgt catgaaggaa aaggccatgg aagtgccagc tatcaaggcc atcatcgaga
    20041 agatggaaga agaaatggcg gggaacggcc gtatccttgt tcgtccaagt ggaacagaac
    20101 ccctcttgcg tgttatggca gaagcgccta caacagaaga agtagactac tatgttgata
    20161 ccatcacaga tgtagttcgt gctgaaattg ggattgacta aatcctagaa aactagatga
    20221 aaataaaaaa ttatggtaca atagcttata atattgtagc cgatggagaa agacatgaat
    20281 cttaaacgtg aacaagaatt tgttagtcag tatcattttg atgcgcgtaa ttttgaatgg
    20341 gaaagtgaaa atggagctcc tgaaaccaag gtggatgtga atttccaatt gctccaacat
    20401 gaccaagaaa atcaagtgac ttccttgatt gtcatcttga gctttatgat tgtatttgat
    20461 aagtttgtca ttagtggaac aatctcacaa gtaaaccata tcgatggccg tattgtcaat
    20521 gaaccaagtg aattgaacca agaagaggtg gaaaccttgg ctcgtccatg tttgaacatg
    20581 ctcaatcgtt tgacttacga agtgactgaa attgcattag acttaccagg aattaatttg
    20641 gagttctaat atgaaattag ctgttttcac agattcctct gcttatctca gtgaagagac
    20701 cttgcaaaga gaagacttgt ttgtcttgga tattcctgtc aatattgatg gtgaggaata
    20761 tgtcgaaggc atcaatctgt ctgctgagga attttaccaa aaaatggctc aggcttctga
    20821 attgcctaag accagtcaac caagtattgc caagttagat gagatcttaa cttcgctcaa
    20881 agaacaaggc tatacacatg ccttggggct tttcctatct tctggaattt caggttttta
    20941 ccaaaatatc cagtatatgg tcgatgacta tgagggctta accattgctt tcccagacac
    21001 tttgattaca agtgctcccc taggcatcat ggttgaaagc gtctttaatt ggcgtgatca
    21061 gggcgatgat tttgccagca ttcaggataa gctagccatt caaatcagcc gtacgtcagc
    21121 ctttatcatg gtagatgact tggatcattt ggtaaaaggt ggacgccttt caaatggggc
    21181 tgccattttg ggcaatttgc ttagcattaa gccaatcctt tattttaacg atcaaggtgt
    21241 gattgaagtt tacgaaaaag ttcgtactga aaagaaggcc accaagcgct taattgaaat
    21301 tatcaaggaa acaacggctt caggtcaata ccgggtcatt gtcattcatg ggaatgctcc
    21361 tgaaaaggct gaagaattgc gtcagcactt gcttgatttt ggcttgggtt cggatgtttc
    21421 acttgctaca tttggtagtg tcattggaac gcacctagga gcaggaagta ttgctctggg
    21481 ttatattcca gtgatttagt tggcgcttct aggctagtta agatgccttg tatcttagaa
    21541 attgaacacg gactacctgc ctcttgaaaa aagatgcctg tcttgccttt cgtggaaagt
    21601 cagtgccatt ccctattttt cagaggcagc taaggtcctt tgtatcttga taattgaaca
    21661 cgcctggaac cctgtgtgaa aaagatagtt cttccaagaa gtagacactt cttgattgga
    21721 attcctattt tcactttgtg ttcttacgga ctttgtatct tagacaggag tagagatgag
    21781 tattcgagta attattgccg gttttaaggg aaagatgggc caggctgctt gtcagatggt
    21841 attgactgat ccagacttgg acttggtagc agttttggat ccttttgagt ctgagtcaga
    21901 atggcagggt attcctgttt tcaaggataa ggctgattta gctggttttg aagcggatgt
    21961 ctgggtagat tttactactc cagctgttgc ctacgaaaat acacgttttg ctcttgaaaa
    22021 tggctttgct ccagtagttg gaacgactgg tttcacgagt gaagaaattg cagagttaaa
    22081 agaattttct cgtgcccaag acttgggtgg cctgattgcc cctaactttg ccttgggtgc
    22141 tgtcttactc atgcaatttg cgacgcaggc tgccaaatat ttcccaaatg tggagattat
    22201 tgagctccat catgacaaga aaaaggatgc tccgagtgga acagccatta aaacagctga
    22261 gttgatggca gaggttcgag agtccattca gcaaggcgca gcagatgagg aagagctgat
    22321 tgctggtgct cgtggtgctg actttgatgg tatgcgcatc cactcagttc gtttgccagg
    22381 cttggtagct catcaagaag tcatctttgg caatcaggga gaagggttga ccctccgtca
    22441 tgactcctat gatcgcatct ccttcatgac aggagtcaat ttgggaatta aagaagttgt
    22501 caagcgtcat gagcttgtct atggattaga acacttatta tgagattaac gcaaatgcct
    22561 tctgaatttc agaaggcttt accagtatta gaaaaaatta aagaagcagg ctttgaggct
    22621 tattttgttg ggggctctgt tcgagatgcc cttctccata gccctatcca cgatgtggat
    22681 attgcgacgt cttcttatcc agaagagacc aagcagattt ttccgcgaac agccgatatc
    22741 ggaatcgagc atggaaccgt cttggtctta gatggggatg aggagtatga ggtaacaacc
    22801 tttcggacag aggatgtcta tgtggactat cgcagaccca gtgcggtttc ctttgtgcgc
    22861 tcgctagaag aagacctcaa acgccgtgat ttcacagtca acgcctttgc cttggatgag
    22921 acaggagaaa tcgttgactt gttccatggt ttagaagatt tggaaaagca agtcttgcga
    22981 gcagttggag tggctagtga gcgtttcaac gaagatgctt tacggattat gcgtggtttc
    23041 cgttttcagg ctagtcttgg ttttgcactt gagccagaaa catttaaagc tatgaagacc
    23101 ttgacgccgc ttttggagaa aatttctgta gagcgtacct tcgtcgagtt tgataaactc
    23161 ttgctggctc cattttggag aaggggcttg gcttccatga ttgagagtca agcttatgac
    23221 tatctccctg atatggcatc tagccaggac aagctcaaca gactgtttga tttggagact
    23281 gattttactt ttgaatcctc tgaacaagcc tgggcggctt tactatgggc tttggagatt
    23341 gaaaatgcgc agtcattttt gaaatcttgg aagacctcac gccagtttgc caagcaagtt
    23401 caggatttgc tgattatttt ggctctgcgt gaaaatggag aattgagcaa gcgagattgt
    23461 tatcgctttg atatagattt gcttttacag gctgaaaatc ttcgtcaagc tcaaggaaaa
    23521 gaagtcaacc tacaagccat cacagaaaaa taccaaagct tgactatcca tgacaagaaa
    23581 gagattcaga ttaatggcga tattttgatc aaggaatatg gctatcagcc aggcccagac
    23641 ttgggagaga ttttaacaga gattgagttt gccattgtcg atggagaatt ggagaataat
    23701 cgtgaagcca tccatgctta cctgagggag aaaaaatgag tgattttatc gttgaaaaac
    23761 taagtaaatc cgttggtgac aagaccgttt ttagggatat ttcctttatt atccatgact
    23821 tagacagaat tggtttaatc ggtgtcaatg ggactggcaa gaccaccctt ttggacgtcc
    23881 tttctggtgt ttctggattt gatggggatg tcagtccttt ttcagctaaa aatgattacc
    23941 agattggtta cttgactcag gatcctgatt ttgatgatag aaagacagtt ttggatacgg
    24001 ttctatctag tgaactcaag gaaatccagc tcattcgtga gtatgaattg attatgctcg
    24061 actatagtga ggacaagcag gcgcgtttgg aacgtgtcat ggcagagatg gactctctcc
    24121 aagcttggga aatcgagagt caggtcaaga ccgttcttag taaattgggc attcaagact
    24181 tatctactcc tgttggggaa ttgtcaggtg gtctgagaag acgggtacag ttggcacaag
    24241 tcttacttgg caaccacgac cttttgcttt tggatgagcc gaccaaccat ctggatattg
    24301 cgattattga gtggctgacc ctctttttga aaaattctaa gaagaccgtc ctttttatca
    24361 ctcacgatcg ttatttctta gacgctttgt caacacggat tttcgagttg gagcgagcag
    24421 gcttgacaga atatcagggc aattaccagg actatgttcg ccttaaggca gaacaggacg
    24481 agcgcgatgc agctctactt cataaaaaag aacaactcta caaacaagaa ctggcctgga
    24541 tgcgcagaca accgcaggca cgtgcgacca agcaacaagc tcgtatcaat cgtttccatg
    24601 atctgaaaaa ggaagtttca ggcagtagtg ctgagacaga cttgactatg aactttgaaa
    24661 ccagtcggat tgggaagaaa gtcatcgagt ttcaggatgt ttcctttgcc tatgaaaaca
    24721 agcccatttt gcaagatttt aatctcttgg tacaggtcaa agatcgtatc ggaatcgttg
    24781 gggacaacgg tgttggaaaa tcaaccttac tcaacttaat tgcaggaagt cttgagtcaa
    24841 ctaaaggaca agttgtgatt ggggaaactg ttcgcatcgc ctatttctct cagcaaattg
    24901 agggcttgga tgaaagcaag agagttatca attacctgca ggaagtggca gaggaggtca
    24961 agaccagtgg tggttctacg acttcgattg cagagttgct ggagcagttc cttttcccac
    25021 gttcgacgca tggaaccttg attgagaaat tgtcaggggg tgagaaaaaa cgtctttatc
    25081 tcctcaaact gcttttggaa aaaccaaatg ttcttctttt agacgagcca accaatgacc
    25141 tagatattgc aactttgaca gtcttggaga atttcttgca aggttttgca ggtcccgttt
    25201 taacagtcag tcacgaccgc tatttcttgg ataaggtagc gaccaagatt ctcgcttttg
    25261 aggatggcaa gattcgtcct ttctttggtc attacaccga ctatcttgat gaaaaagctt
    25321 ttgaaacaga tatggccaat caagtgcaaa aggccgaaaa ggaaaaagtg gtcaaggttc
    25381 gagaagacaa gaaacgcatg acctaccaag aaaagcagga gtgggcaagt attgaaggtg
    25441 atattgaaac cttggaaaaa cgtatcgctg ctattgaaga ggaaatgcag gctaacggct
    25501 ctgactttgg taagctggct actctccaaa aagaattgga tgagaaaaat gaagcactcc
    25561 ttgaaaaata cgaacgctat gagtatctca gtgaatttga tagttaagac tattcgaaaa
    25621 tcaaagtgca aactatgtcg gcttcacatt gccgactcaa gtacagcctg tgaccggctt
    25681 cctaatttgc actttgattt ttattgagta taaaagaata gaaaaatccc gtctcatgaa
    25741 aggatttctc tattcttttt tggtttcttg atgaaagata ttttgccagg tttcgtggcg
    25801 acgccagata ctggtatgga tgataccatc taactcatag cagatgagtt tggttttttg
    25861 actgatggaa gtaatttgaa tgtccttgat agcagaattt aactcttttt tggtcttata
    25921 agcctcttta tccatctgct ctccatcttg gcgaatatag acaaaatcat cagtcaagag
    25981 ttcttctagt tggtttcctt ggtcaatcaa ttgttcacgc ataagcaatt ttttatagac
    26041 attgtctaaa atttcatcct cagggatagg tgctggttcg atatggacat cggtatcaaa
    26101 gacgccaaaa cgctcctcca gcatagactc gacctgatcc gcgatttcat ggctttcaaa
    26161 aacagacaag tcaggattca tctctagtgt aatatccagg tagatgttgc taccgtaggt
    26221 gcgacctctt tgcgatttga ccttgctgat tttgggaatt tccatgatag ccttttggta
    26281 gtcctcgagc aggcggtcgt caaagccatc tgaaagacta aaggaagact cgatgaagat
    26341 atcataggca gtcttcaaga taaagaaagt gatgatgata gcaaccagtt tatccacaat
    26401 cggataattg aaactactag ctaggatggc aatggcggtt ccaagtgagg taacagcgtc
    26461 agaaagattg tccttagcag ctgccttcag cgccttggag ttggatttct tactgaggcg
    26521 agtattgtag agatagacca caaacataat cgctgcagaa atgattccta gagttgcacc
    26581 aagaggatca atgaccgttt cttcccgact gagaatcttt tgaatggtat ctcttagaac
    26641 atcgaaaccg acatagaaca tgatgataga agtgatcaag cttgccaaat cttcaatctt
    26701 ccaatgacca aaacggtggt cacggtctgc aggctggcgc gccatccgaa tcccgattaa
    26761 gagggccaca tttccaatga tgtccgatac gttattaaaa ccatcggcca ccaaactgga
    26821 tgaatgaagg agatgaccag ctgctaattt ggctgcagac aagatcaaat aggtcgaaat
    26881 actgataatg gctccacgct cagctaactt gagatttgag atagattgct tcattcaact
    26941 tcctttctag tcacatagca ttctaccatt atactggttt tcaaggaaaa attcaaataa
    27001 ggcagttttt actctttgaa aactccccaa aatttggtat aatggtacta ctcaagggag
    27061 tagctggcag aaacctgtga tagtgtcgtc attccgaatt ttatgctgaa aagtatgctt
    27121 tccggcccta tcttaaacag cgagacttgt tatgattaac aggtctcttt ttgtttgtcg
    27181 tgaaaagata tgacagaaag agagtctgtt ttgcacacaa tgtcaaggag gagacacatg
    27241 tcaaaagaac aaaaacgcca agcgttttat actcagagcc ctgaagaggt cttgcaggct
    27301 gtggatgcga ccgagcaagg tttgtcatca agtgaggcgg aaaagcgcct tgccgaattt
    27361 gggcacaatg aactcgaaga aggcgagaaa cgatcaatct tggtcaaatt catcgagcaa
    27421 tttaaggatt tgatgattat catcctagtt gcggcagcaa tcttgtcagt cgtgacttct
    27481 ggtggggaag atatcgcaga tgccattatc atcctagctg tggttatcat caacgctgcc
    27541 tttggtgttt accaagaagg aaaagctgaa gaagctattg aagccctcaa atccatgtct
    27601 agtccagttg cccgcgttct tcgtgatgga cacatggcag agattgactc taaagaattg
    27661 gtaccaggcg atatcgttgc ccttgaagca ggtgacgtgg taccagcgga cctacgtttg
    27721 ctagaagcca actctcttaa aattgaagaa gcagccttga cgggtgaatc tgtaccagtc
    27781 gaaaaagact tgtcagtcga gcttgcgaca gatgctggta ttggtgaccg tgtcaacatg
    27841 gccttccaaa actcaaacgt aacctatggt cgtgggatgg gtgttgttgt caatacaggt
    27901 atgtacactg aagttggtca tattgctggt atgcttcaag atgcggatga gactgataca
    27961 ccactcaaac aaaacttgaa caacctttct aaggtcttga cctatgctat cttggtcatt
    28021 gcccttgtta cttttgtagt gggtgtcttc attcaaggga aaaatccact tggtgagttg
    28081 ttgacttctg ttgcccttgc tgttgcagcc attccagaag gacttcctgc tatcgttacc
    28141 atcgttcttt ctcttggtac tcaagttttg gccaaacgac attccatcgt tcgtaagttg
    28201 ccagcagttg aaacacttgg ttcaactgaa atcatcgctt ctgataagac tggtacgctg
    28261 actatgaaca agatgacagt cgaaaaagtc ttctacgatg cggttctaca tgactcagct
    28321 gatgatattg aactaggtct tgaaatgcca ctacttcgtt cagttgtctt ggccaatgat
    28381 acgaaaatcg atgtggaagg taacttgatt ggtgacccaa ccgaaacagc ctttatccaa
    28441 tatgccttgg acaagggcta tgatgtcaaa ggtttcttag agaaatatcc tcgtgtagct
    28501 gaattgccat ttgactctga ccgtaagctc atgtcaacag ttcacccatt gccagatggt
    28561 cgtttccttg tagcagtcaa gggtgcgcca gaccaactct taaaacgttg tcttcttcgt
    28621 gataaggctg gggatattgc tccgattgat gagaaggtta caaatctcat tcatacaaac
    28681 aattctgaaa tggctcatca agccttgcgt gtccttgcag gtgcttataa gattatcgat
    28741 agtattccag aaaatctcac ttctgaagag cttgaaaatg atttaatttt tactggtttg
    28801 attgggatga ttgatcctga acgtcctgaa gctgctgagg ctgttcgtgt ggctaaggaa
    28861 gctggaattc gtccaattat gattacaggt gaccaccaag acaccgcaga agctattgcc
    28921 aaacgtttgg gaatcattga cgcaaacgat acagaagacc acgttttaac tggtgctgag
    28981 ctcaacgaat tatctgatga agactttgaa aaagttgttg gtcaatactc tgtttatgcc
    29041 cgtgtgtctc cagagcacaa ggttcgtatc gtcaaggctt ggcaaaaaca aggtaaggtc
    29101 gttgccatga caggtgacgg tgtcaatgat gccccagctc tgaaaacagc agacatcggt
    29161 atcggtatgg gaatcactgg tacagaggtt tctaaggggg cttctgatat gattcttgca
    29221 gatgataact ttgcgactat tatcgtcgca gtggaagaag gacgtaaggt cttctcaaat
    29281 attcaaaaga ctattcagta cctactttct gccaatactg ctgaagtatt aaccatcttc
    29341 ctatcaacct tgtttggttg ggacgtcttg cagccagttc atcttttgtg gattaacttg
    29401 gtaacggata ccttcccagc aattgcacta ggtgttgaac ctgcggaacc tggtgtcatg
    29461 aatcataaac cacgtggacg caaggcaagc ttcttctcag gtggtgtttt gagttctatc
    29521 atttatcaag gtgtactcca agcagctctt gttatgagtg tttatggcct tgcgattgct
    29581 tacccagttc atgtgggtga caatcatgct attcatgcag atgccctaac gatggccttt
    29641 gcaacccttg gtttgattca gctcttccat gcctacaatg tcaagtctgt ctaccaatcc
    29701 atcttgacag ttggcccatt caagtctaag acctttaact ggtccatctt ggtatccttt
    29761 atccttctca tggcaacaat cgttgtagaa ccgcttgaag gaatcttcca cgtaaccaaa
    29821 ctagacttgt cacaatgggg aattgttatg gctggaagct tctcaatgat tatcatcgtc
    29881 gaaatcgtta agtttattca acgcaaactc ggttttgaca agaatgcgat ttaagggagg
    29941 tttcggatgt cagcatatca attaccgacc gtatggcagg atgaagctag taatcaagga
    30001 gcttttacgg ggctaaacag accaacagta ggtgcccgtt tcgaacaaaa cttgccaaaa
    30061 ggagaacaag cttttcagct ttattcactg ggaacaccaa atggtgtgaa ggttactatc
    30121 ttattggaag aattactaga agctggtttt aaggaagcgg cttacgactt gtataagatt
    30181 gctatcatgg atggggatca attcggatca gactttgtga agctcaatcc aaattccaag
    30241 attccagcct tattggacca gtcaggtact gaagatgtaa gagtctttga gtctgctcat
    30301 attcttcttt accttgctga gaaatttgga gcctttttac caagtaatcc tgtggaaaag
    30361 gtagaagttt tgaattggct attctggcaa gcaggtgcag caccttttct aggtggggga
    30421 tttggacatt tcttcaatta tgctcctgaa aaattggaat atcctattaa ccgttttaca
    30481 atggaagtga aacgccagtt ggatttattg gataaggaat tggctcagaa accttatatt
    30541 gcaggcaatg actatacgat tgcagatatt gctatctggt cttggtatgg acagttagtt
    30601 caaggaaatc tttaccaagg ttctgcaaaa ttcttggatg cctcaagtta tcaaaatcta
    30661 gtaaaatggg cagaaaaaat tgccaatcgt ccagctgtta agcgtggctt ggaagtaact
    30721 tatacagaaa ttaaatagca aaaaagtcat cttcggatgg ctttttgcta cagttgaggg
    30781 aaagggtttg cagttatcgg cttttgtgct ataattgcta taatatagta aaaagcaaga
    30841 ggagtgtgag gaagtggaaa agaaaattgt gaaggatatc ttatttttat ctcaagtgtc
    30901 tcagccggca agtcaggagg acctttatct tgccagagat ttgcaggata cactcttagc
    30961 aaatcatgat acctgtgttg gtctagctgc caatatgatt ggggtgcaga agcgcgtgat
    31021 tatctttaat cttggcttgg ttcccgtggt catgtttaac ccagtgcttc tgtcctttga
    31081 aggatcctat gaggcagaag aaggctgttt gtccttggta ggcgtgagat caactaagcg
    31141 ttatgaaacc ataagggttg cctatcgtga cagcaagtgg caggaacaga ccattacctt
    31201 gacaggcttc ccagctcaga tttgccagca tgagctggat cacttggaag gacgaatcat
    31261 ttaggaggaa agcaaatgaa acgaatagtc tttgaactta tttttatcgc aacgacctgg
    31321 tatatctttt taccgcccct taacctgacc agctgggaat ttctcttctt cctctgtggg
    31381 catttgttag ttgtggcaat cttatttggc tttggcaagg ggataaacct tgtcaaaacg
    31441 gttcatgtgc gccacggtaa ggcggaagct gccttaaatc ttgagggttt caaaatcaat
    31501 cggttaggga aaattctgtt agcttcgatt ggaggaattc ttctcttggc agctttggtt
    31561 tccttggtaa cttccagcat gtttcaggct aaaaattatg ccaatgtagt cacggttacg
    31621 gaaaaagact ttactgaatt tcctaggagt gacaccagta aggttcctat cctagataga
    31681 agtactgctg aaaaaattgg agaccgctac ttgggttccc taaccgataa ggtgtcgcaa
    31741 tacgtagcgg cagataccta tacccaattg acaattgatg ggaaacctta tcgggtcaca
    31801 ccactagaat atgcagaccc tatcaaatgg tttaacaatc aagccaaggg aatcggtgag
    31861 tatatcaagg tggacatggt aactggaaat gcggatttgg tggacttgaa gacaccaatc
    31921 aagtattcag actcggagta ttttaaccgt gatgtcaaac gtcacctgcg cttgaagtac
    31981 ccgaccaaaa tctttaaaac tccatctttt gaggtggacg atgagggcaa tcctttctat
    32041 gtagcaacgg tttaccaaaa gcaatttgga cttgctgttc ctcgtcctgc ttcagtcatt
    32101 atcttggatg ctacaaatgg agaaaccaag gaatacagct tatcagatgt tccagaatgg
    32161 gtggacagga tctatccagc agaggaaacc attgagcaaa tcaactacaa cggcaagtac
    32221 aaggacggtt tcttgaatgc catgatttcc aagaaaaacg tgacccagac taccaagggc
    32281 tataattact tgtctatcgg taatgacatc tatctctaca caggtgtgac gtcggctaat
    32341 gcggatgaga gtaatcttgg tttcatcctt gaaaatatgc gaacaggaga aatcactaag
    32401 tatagcttgg cttctgcgac agaagaatca gcccgtgaat cagcagaagg tgctgttcag
    32461 gagaaatcct acaaagcaac cttcccaatc ctcatcaacc tcaatgacaa gcctctctac
    32521 atcatgggct tgaaggacaa tgctggcttg gtcaaagagt acgccctggt agacgcagtc
    32581 gagtaccaaa atgttatcgt tgctactaca gtggaagaga tgctcagcaa gtatgccaat
    32641 aaaaacgacc ttgaaattga caatgcaacg acagaaagca tcaatggagt agtagcagac
    32701 ctcaaatcag ctgttatcaa gggagacact gtctacttct ttaaagttga tggcaagata
    32761 tacaaggtca aggcttcagt atccgatgac cttccttacc ttgaaaatgg taaaaccttc
    32821 gaaggtcaag taggaaaaga caattatctc aagaccttta agctacggta aaaataggtt
    32881 tttttcagaa agtatatgtt ataataaggt aaattaagcc taatggagga caaagaaatg
    32941 ggtttttatt tgatggttgc ttcaatgcta ctcggactgc tcgctttgaa gataggtttt
    33001 tctcagttca aggagaaaaa agataaattc ttatctatct taacaagttt agctggcaca
    33061 gcccttgttc tcgtggctgt ctggttagga tggcccaaat aaatagaaaa acctcggtca
    33121 aaccgaggtt tttcaaatga atttcttggt tgtggctaaa aaatatgcta cactatcaat
    33181 atgaaaattt taatcccaac agcaaaagaa atgaacacag acttgccaag tatcgaggca
    33241 atttctttaa aaccagaaag tcaggccgtg cttgatgcct tggctctcta ttctgccagt
    33301 caattggaga gtttctacaa ggtatcagct gagaaagcgg cggaagaatt tcaaaatatc
    33361 caagctttga aaaggcaaac tactcaacac tatccagcct tgaaactttt tgatgggctt
    33421 atgtaccgca acattaagag agataagctg accgaggcgg aacaagatta tcttgaaaat
    33481 catgtcttca ttacctcggc tttgtacggt gttgttccag ccttgtcacc catggctcct
    33541 caccgtttgg attttttgat gaaattaaaa gttgctggta agactttgaa gagccattgg
    33601 aaggcagtct atgatgaagc tctgaagaag gaagaagtga ttttctctct cttgtcatca
    33661 gagtttgaga ctgtattttc taaggaaatc agagcaaaga tggtgacctt caaattcatg
    33721 gaggatagag gcggtcagct gaagattcac tcaactatct ccaagaaagc gcgcggggcc
    33781 tttctaacag ctttaataga aaatcaagta caaactgtgg gggaagcacg tcgcttgaac
    33841 tttgctggat ttgtttaccg agaagatttg tcacaaccac agggattggt ttttgtaaag
    33901 gaagtataga aatcagatat tttgtatttg gcaatatgta tcatcagcta ttttagttta
    33961 taaatgagaa aaaagcgaac aagctagtct tctgagcgtc agaaaagtag tcttgttcgc
    34021 ttttattatg ttatactctt cgaaaatctc ttcaagccat gtcagtgtcg ccttaccgta
    34081 tatatgttac tgacttcgtc agttctatct acaacctcaa agcagtgctt tgagtagctt
    34141 gcggctagct tcctagtttg ctttttgatt tttattgagt gttagttact tgatagcttc
    34201 gaccaagtct tgagcttgtt tttcaagtga gtttaggact gtttcttcaa gaaccaattt
    34261 tccgtctgcc caggcagagt cattaacacg tgcagcagtg aaatcaccaa cgccttgtgt
    34321 acggataaat ggcaagaggt ctttgtagat agcgaaaagt tgatcgtgcc ctgcattggc
    34381 tacagatgat actgtgacaa acttgtcttg aagggcagaa acgccacgtg tatcagacaa
    34441 gtcaagggca cgagatagcc agtcaagcaa gtttttcact gtaccaggga tagagaagtt
    34501 gtagactgga gagaaaatcc agatagcatc cgcaacgaga actgcttcac gagcagcagc
    34561 tacagctgga tgtgttggaa cttccaaatc ttggctaaag agaggaaggg ctgaataatc
    34621 aaggtagcta acttccgctt taccagcaag tgctttctca gcttcgagcg ccatttggtg
    34681 gttgaaagaa ccttgacgta gtgatccgac gataaataat acttttttag acatgatgtg
    34741 tctccttttt cttaaaattt cgagaactct ctcgttgtta taaaatatat tacaacaaaa
    34801 caaaagaact tgcaagaaat ttgctcagag tttatttgaa aattgtaaaa tttcggaaac
    34861 tctaatcatt tgtgactgaa aaagtaagaa ctgtttcact ttcaaaatcg gttttgtttg
    34921 aagggagaaa tagaattggt tgctcaaagc acagctttga ggttgcagat aaagctgacg
    34981 tggtttgaag agattttcga agagtattct tatattcact tgtaataaag cttaatagat
    35041 ggtaaaatag acgagtgaaa aaaagacaaa acaaagcaaa aaataatcta ctgtggcagt
    35101 atggtctagg gatgacgatt ttgtttgtgg ttatcagtgc ttcctttctg tatatggttt
    35161 ctcttagcat gaaaccctat caaacagcta aaagtgaagg agaaaaatta gctcagcagt
    35221 atgcaggatt agagcaggtc gatcaggttg atttatacaa tggcttggaa tcttattaca
    35281 gcgttcttgg tcgtaataaa cagcaagaag cgcttgctgt cctgattggt aaagatgatc
    35341 ataagattta cgtttatcag ctaaatcagg gtgtttcaca agaaaaagca gaaacggttt
    35401 ctaaggaaaa gggagctggc gaaattgaca agattatctt tggtcgttat caagataagc
    35461 caatctggga agtcaagtca ggatctgatt tttatctagt agattttgaa acaggagcat
    35521 tggtcaacaa ggagggccta tgaaattatc caaccgtgtt ttagaaatgg aagaaagtgt
    35581 gactttggct gctggagccc gtgccaaagc actgaaggct gagggtagag atattttgtc
    35641 tctaaccttg ggtgagccag atttcactac tcccaaaaat atccaagatg ccgccattgc
    35701 atcgattcga gatgggcgtg cttcttttta tacagtaacc tcaggtctgc cagagcttaa
    35761 ggcggcggtc aatagctact ttgagcgctt ttacggctat tctgtagcgt caaatcaagt
    35821 gacagtcgct gcgggagcca aatattctct ctataccttc tttatggctg tggtcaatcc
    35881 aggtgatgaa gtcatcatcc caaccccata ctgggttagc tatggagatc aggtcaagat
    35941 ggcagaaggc gttcccgtct ttgtttctgc taaggaagac aatcacttta aggtgaccgt
    36001 agagcagtta gaagcagctc gcactgacaa gaccaaggtt ttggtgctga attcgccatc
    36061 taatcccaca ggtatgattt acacccgtga ggaactcttg gcaattggaa actgggctgt
    36121 agaaaatgat attctcatcc tagcagacga tatctatggc cgcttggttt ataatggtca
    36181 tgagttcaca ccgatttcta gcctatcgga agcgattcgc aagcaaacag tggtcatcaa
    36241 tggtgtgtct aaaacttatg ccatgactgg ttggcggatt ggttatgccg ttggagaagc
    36301 agatattatc gctgccatgt ccaagattgc aggtcaaaca acttcgaatc cgtcagcagt
    36361 agcccaatat gcagcagttg aggctctatc aggtgagcaa gatactgtag aaagcatgcg
    36421 tcaggccttt gaggaacgtc ttaatactat ctatcccctc cttgcagagg tgccaggatt
    36481 tgaagtggtc aaaccgcaag gggccttcta tctcttccca aatgtcaaaa aggccatgga
    36541 gatgaaaggc tacacggatg tgacagactt tacaactgct atcttagaag aagccgaagt
    36601 ggccttggta acaggagctg gatttggagc accagaaaat gtccgcctca gctatgcgac
    36661 agacctagac acgcttaaag aagcagtcga acgcttgaaa gcatttatgg gtagtgagaa
    36721 tgattgatca ttttgagatt aaggtaaagg atttacaaat ttcagaagga ttttatagga
    36781 gttttctcgc tcctttggac tataaattgg cttttaagac tagttctcta attagttttc
    36841 tatccccgaa cagccctcat cctggtggtg atttttggct gactcaggga acacaagatc
    36901 ctgttcattt tgctttttta gcagaaaata aggaagaagt tcaggcttgt tatgaggctg
    36961 gcttagaggc aggtgggcga gacaatgggg ctcctggtta tcgaagtgag cgtctgattt
    37021 actatgctgc ttttatgatt gacctggatg ggaacaatat agaggtggtt tgccataaag
    37081 aataaaaaag aaaaggaaaa ataatgacaa aacgtgtaac gattattgac gtaaaagact
    37141 atgttggtca ggaagtgacg attggcgctt gggttgccaa caaatcagga aaaggaaaaa
    37201 tcgctttctt acaattgcgt gatggaacag ccttctttca aggtgtggct tttaaaccaa
    37261 actttgtcga aaaatttggt gaagaagtgg gacttgagaa gtttgatgtt atcaaacgct
    37321 tgagccaaga aacgtctgtt tatgtgacag gtattgtcaa agaggacgaa cgttctaaat
    37381 ttggctatga gttggacatc acagacatcg aagtgatcgg tgaatctcaa gactacccaa
    37441 tcacaccaaa agaacacgga acagactttt tgatggataa ccgtcacttg tggctacgct
    37501 ctcgtaagca agtagctgtg ttgcaaatcc gtaacgctat tatctatgca acttatgagt
    37561 tctttgacaa gaacggtttt atgaagtttg acagcccaat tctttcagga aatgcggcag
    37621 aagattctac agaactcttt gaaactgact acttcggaac gccagcctac ttgagccaat
    37681 caggtcagct ttacctagaa gcaggggcta tggctcttgg tcgtgtcttt gactttggtc
    37741 cagttttccg tgctgaaaaa tcaaaaacac gccgtcactt gactgagttc tggatgatgg
    37801 atgctgagta ctcatacttg acacatgatg agtcgcttga cttgcaagaa gcttatgtga
    37861 aagctcttct acaaggtgtt cttgaccgcg cgcctcaagc cttggaaacc ttggaacgtg
    37921 atacagaact cttgaaacgc tacattgcag agccattcaa acgtatcact tacgatcaag
    37981 ccattgacct cttgcaagag catgaaaatg atgaagatgc tgactacgag catcttgagc
    38041 atggtgatga ctttgggtca ccacacgaaa cttggatttc aaaccacttt ggtgtgccaa
    38101 catttgtcat gaactatcca gcagccatca aggccttcta catgaaacca gttcctggaa
    38161 atccagagcg cgtgctttgt gcagacttgc ttgctccaga aggctatgga gaaattatcg
    38221 gtgggtctat gcgtgaggaa gattacgatg cccttgtcgc taagatggat gaacttggca
    38281 tggatcgtac agaatatgaa ttctaccttg accttcgtaa atacggtaca gttccacacg
    38341 gaggatttgg tatcggtatc gaacgtatgg taaccttcgc agcaggaaca aaacatatcc
    38401 gtgaagctat tccattccca cgtatgttgc accgtatcaa accataagaa caggaaaacg
    38461 cccatgtggc gttttttcga attctagtgt ttttacttgc gtaaaaaaat tttttctagt
    38521 ataatagttt attgtgagcg aacctcactt accccttgca aagtcttggg gtcattagac
    38581 caaaaggagg aacatatcaa tggctaaata cgaaattctt tatatcattc gtccaaacat
    38641 tgaagaagaa gctaaaaacg ctttggtagc acgttttgac tctattttga ctgacaacgg
    38701 tgcaactgtt gttgaatcaa aaacttggga aaaacgtcgt cttgcatacg aaatccaaga
    38761 tttccgtgaa ggactttacc acatcgttaa cgttgaagca aatgacgatg cagctcttaa
    38821 agagtttgac cgtctttcaa aaatcaacgc tgacattctt cgtcacatga tcgtcaaaat
    38881 tgacgcgtaa gaaggtaaac tatgattaac aatgttgtac ttgtagggcg tatgacacgt
    38941 gacgctgagt tgcgttatac cccatcaaat gtagcagttg cgacttttac tcttgcagta
    39001 aaccgtacat ttaagagtca aaatggtgaa cgtgaggctg attttatcaa tgtcgttatg
    39061 tggcgccaac aggctgaaaa tcttgctaac tgggctaaaa aaggctcact tatcggggtg
    39121 acaggtcgta tccagactcg tagttacgat aaccagcaag gacaacgtgt ctacgtgaca
    39181 gaggtcgtgg ctgagaattt ccaaatgttg gaaagccgta gtgtgcgtga gggccacaca
    39241 ggtggagctt actctgcacc aactgcaaac tattcagcac ctacaaattc agtaccagac
    39301 ttttcacgta atgaaaatcc atttggagca acaaacccat tggatatttc agatgatgat
    39361 ttaccattct aatggacaac taaacttata aaggagaaaa aacatggctc aacaacgtcg
    39421 tggcggattc aaacgccgta aaaaagttga ttacatcgca gcaaacaaaa ttgaatatgt
    39481 tgattacaaa gatactgagc ttcttagccg tttcgtttca gaacgtggga aaatccttcc
    39541 tcgtcgtgta acaggaactt cagctaaaaa ccaacgtaaa gtaacaacag ctatcaaacg
    39601 cgctcgcgta atggctttga tgcctttcgt aaacgaagat taaagaaaag acccggttgg
    39661 gtctcttttt catgagcttg aaaacaagaa agcgaattaa gaccccaacg ggtctttttt
    39721 cacaagcttt gaaatgagag agcgagttag gctcagtggc tcccttttta cgagtttgaa
    39781 aacaagagaa cacattaaaa gcacgatgga gctttttcat aaatatgtct tttttctcat
    39841 ccaagaaatt aaaataaaaa tgataaactt tttgaaaata ggggatttgc ctactttatg
    39901 atataatgga aagagttaga ttgaaagagg tagtgagatg gacgcgaaat taagatacaa
    39961 ggcaaagaag atcaagattg tcttttttga tattgatgat acattgcgga attcaaagac
    40021 agggtttatt ccaactacaa ttcccactgt ttttaaacag ttgcgtgaaa aaggaatttt
    40081 aacaggaatc gcctctggac gtggcatttt tggtgttgtt ccagagattc gtgatctcaa
    40141 gcctgacttt tttgtaactt tgaatggagc ttatatcgaa gataaaaaag gtcaggtcat
    40201 ttatcagcat cagattgaaa agtcagatgt tgaggagtat atctcttggg ctaagcaaga
    40261 aggaattgag tatggcttgg ttgggagtca tgatgccaag ttgtcgactc gcaccgatat
    40321 gatgagtgaa gctatcaatc caatttatcc cgacttagat gtagatcccg atttccatga
    40381 aaaagaagat atctatcaga tgtggacttt tgaagataag ggagatgact tgcacttgcc
    40441 tgacagtctc tcagacaaac ttcgcatggt tcgttggcat caacattcgt ctgatattgt
    40501 gccgatttca ggctccaaag cgacgggggt ggaaaaggtt gtggaacacc ttggcttgaa
    40561 accagagaag gtcatggttt ttggagatgg tctcaacgac ttggaactct ttgattatgc
    40621 tggaatcagc gttgccatgg gaatttctca tgataaaatc aaagaaaaag cagattatat
    40681 tacaaaaaca ttagaagaag atggcatttt tgatgcctta gaagtatttg gtatggtaga
    40741 aaaagaattg cattttccac aagtagacat tgaaacagta gaaggtcctc ttgcgactat
    40801 taagaccaat cacggagact tacgtatcaa gcttttccct gaacatgctc ctaaaacagt
    40861 ggctaacttt gtatctcttt caaaagatgg ctactatgat ggtgtcattt tccaccgtat
    40921 tatcaaggac tttatgatcc aaggtggaga cccaactgga actggtatgg gtggcgagtc
    40981 aatctacggc gaatcttttg aggatgaatt ctcagaagag ctttacaata tccgtggtgc
    41041 tctttccatg gcaaatgctg gtccaaatac caacggcagc cagttcttta tcgtgcaaaa
    41101 ccaacaccta ccttattcga agaaagaaat tactcgtggt ggttggccag aaccgattgc
    41161 agaaatctat gccaatcaag gtgggacacc tcacctagac cgccgtcaca cggtttttgg
    41221 tcagttagct gatgaagcat cttacgctgt cttggatgcc attgctgctg ttgagacagg
    41281 agctatggac aagccagttg aagatgttgt aattgaaact attgaaatcg aggactaaga
    41341 tgaaaatcgg tgataagcta aaggggcgta ttacagggat tcagccctac ggtgcctttg
    41401 ttgagttaga gacgggtgat acggggctga ttcacatttc agaaattcgg acaggattta
    41461 ttgaaaatat tcatgaaacc ttgaaagtcg atgaagaggt tcaagttcag gtagtggatt
    41521 tagatgaatt tacggggaaa gccagtcttt ctatccgcac tttggaggaa gaaaagtacc
    41581 agtttcctag acggagacgc ttttcaagcg accgctttaa ctacggtttt gctccctttc
    41641 gacgaatgct accaatctgg actggagaag ccctgcacca tttaaaaaag aagaagtagt
    41701 ttagagtttt atattttttg taatgttaat atgaatttgc tataatagga ccatggaaga
    41761 agaacaatta ttaaaatcag gagagcgcat taaccagctc ttttcgacag atatcaaaat
    41821 cattcaaaat agagaggttt ttagctattc ggtggatagt gttctcttat cacgatttcc
    41881 acgttttcct aagaaggggt tgattgtgga tttctgtgct gggaatggag cagtggggct
    41941 ttttgctagc actcgtactc aagcacagat attgtctgtt gagattcagg agcgtttggc
    42001 ggatatggct gaacgctctg tccgtttgaa tggattagag gagcaaatgc aggtcatctg
    42061 cgatgatttg aaaaatatgc ctgctcacat tcaggggagt aaggtggata tgattttgtg
    42121 taatccaccc tatttcaagg tgaatcctta ttctaatctg aacgagagtg aacattatct
    42181 cttggctcga cacgaaatca cgactaattt ggaagaaatc tgtcgtagtg cccagagtat
    42241 tctcaagtct aatgggcgtt tggccatggt tcatcgtcct gatcgacttc tggatatttt
    42301 ggatacgtta aaacgacata atctagcccc taagcgcctg cagtttgttt atccaaaaag
    42361 agaaaaggaa gccaatatgc ttttgattga ggcgatcaag gatggctcaa caagtggctt
    42421 taaggtctta cctcctttga ttgtccacaa tgatgatggc tcttatacgc ccgaaatcga
    42481 agagatttat tatggatcat aaggcttata tgtacgttct ggagtgtcgt gatggatcct
    42541 actatatagg ctatacgact gatatgagaa gacgcctcgc tatccacaat agtgggaagg
    42601 gagccaaata tacacgagca cgcttgccag tcaaacttat ctatgctcaa ggttttgcca
    42661 gtaaggaaga agccatgtcg gctgaagctc ttctcaagcg taagaagagg ccacagaagg
    42721 aagaattttt atctgaaaat caagatagaa atttactcag ttattttgaa gaatcgtggg
    42781 gagtcctttg actcctctta cctttgtcaa aaagcgaaaa aaatgctaca ataaagaaaa
    42841 taaacaaaat gaggtattat catgtccaag attctagtat ttggtcacca aaatccagac
    42901 tcagatgcca tcggatcatc tgtagctttt gcctaccttg caaaagaagc ttacggtttg
    42961 gatacggaag ctgttgccct tggaactcca aatgaagaaa cagcctttgt cttgaactat
    43021 tttggtgtgg aagcaccacg tgttatcact tctgccaaag cagagggggc agagcaagtt
    43081 atcttgactg accacaatga attccaacaa tctgtatcag atatcgctga agtagaagtt
    43141 tacggtgttg tagaccacca ccgtgtggct aactttgaaa ctgcaagccc actttacatg
    43201 cgtttggagc cagttggatc agcgtcttca atcgtttacc gtatgttcaa agaacatggt
    43261 gtagctgtgc ctaaagagat tgcaggtttg atgctttcag gtttgatttc agataccctt
    43321 cttttgaaat caccaacaac acacccaaca gataaaatca ttgctcctga attggctgaa
    43381 ttggctggtg tgaacttgga agaatatggt ttggcaatgt tgaaagctgg taccaacttg
    43441 gctagcaaat ctgctgaaga attgattgat atcgatgcta agacttttga actcaacgga
    43501 aataatgtcc gtgttgccca agtgaacaca gttgacatcg ctgaagtttt ggaacgccaa
    43561 gcagaaattg aagctgcaat gcaagctgcc aacgaatcaa acggctactc tgactttgtc
    43621 ttgatgatta cagatatcgt caactcaaac tcagaaatct tggctcttgg tgccaatatg
    43681 gacaaggtcg aagcggcttt caacttcaaa cttgaaaaca atcatgcctt ccttgctggt
    43741 gccgtttcac gtaagaaaca agtggtacct caattgactg aaagctttaa tgcgtaagat
    43801 tttgggtgtc agctcaaaat cggaaagtct agtttgcctt atatcgaaag gagtttcggc
    43861 tccttttttc taggagtgaa gtatgttaga aaatggcgat ttgatttttg tgagagatgg
    43921 gtcagacatg ggacaggcca tccagacttc cacaggtaac tatagccatg ttgccattta
    43981 tttggatggg atgatttatc atgctagtgg acaggctggt gttgtctgtc aagaaccggc
    44041 agacttcttt gagtccaatc atttatacga cctctatgtt tacccagaaa tggatatcca
    44101 gtcggtgaag gaaagagctt gcaaacatct tggagcaccc tacaatgctt ctttctatcc
    44161 agatgcacct ggtttctact gctcccagta tatagcagaa atcctaccta tttttgaaac
    44221 tattcctatg aaatttggag atggggagca ggcgattagt gatttttgga gggagtatta
    44281 catagaacta ggtctgcctg ttcctctgaa ccaagctggt accaatccta gtcagttggc
    44341 agcatcgcct ctgttacaat gtaaagaaag gaatcttcat gattcagatt tttaatccat
    44401 ctcgtttgac gagacagcca tttttggaga attgatccgc tatctggacc agtatgagga
    44461 tgtgattcta cgggaaatta aggctcaatt tccagatgtt gcagttgata aactcatgga
    44521 agagtatata aaggcaggct tgattctacg tgaaaataag cgctattacc tcaattttcc
    44581 tacgcttgaa tcacttgata gtcttgaact ggatcaagag atttttgtca gagaagctag
    44641 tccggtctat caagccttgt tggagcagag ttttgagacg gaattgcgca atcaaatcaa
    44701 tgcagctatt ttagttgaaa agacggactt tgcgcgcatt aaaatgactc tgtccaatta
    44761 tttttacaag gtcaaacagc agtatccttt gacagaaaaa cagcaggagc tctatgacat
    44821 tttaggagat gttaatcctg agtatgcact caagtatatg acggcttttt tgttgaaatt
    44881 tctcaaaaaa gaccagctta tgcagaaatg ccgtgatatc tttgtggaca gtttggttgt
    44941 cttaggctat attgtgcaaa atgaagatgg aaagtatgag ttggctatcg attttgataa
    45001 ggagagatta actttctact tagcgtgatt tcttgtttct gagtacattg tttgactttc
    45061 cttagtattc ggtataaact atatgtaacc ggtaacacat atcggaataa actaaaggag
    45121 acaatcatat gtcacttgaa aacaaattgg aacaagcaac aggcgctgtc aaagaaggtt
    45181 ttggtaaagt tactggagac agcaagacag aacttgaagg agctgttgaa aaaacagttg
    45241 ctaaggcaaa agacgttgta gaagacgcaa aaggtgctgt agaaggtgcc gttgaaggtt
    45301 tgaaaaacgt ttttactaaa gaataggaaa aaatcaaggg tttcattttc ccttgatttt
    45361 ttctattctt ataaataatt ttctgcgacg gctgtatctc ctgggtagga ttctttcttg
    45421 ccctggatga tttggtaaca atcggctccc ttacccgcaa taataactgc atctaattcg
    45481 tgatttgtga tagccattgc cgccttgatg gcttcttggc gatccgcaat cttctcaaca
    45541 ggatgattga tgtagctact aatttcatct gcaatggcca ttgggtcttc atagttaggg
    45601 tcatcagcag tcagaaagac ttgaatctca gggtgttgat tgaggaggag gccaaagtcc
    45661 ttacgacgac tttctccctt gtttcctgtt gatcccagaa ccagagcaat ctttccggtt
    45721 tgatgagttt caaccacatt gatgagtttt ttcagactat ccccattgtg ggcatagtcg
    45781 atgaagacct tggctccatt tttctgagtg aggacttcca tacgaccagg aacgcgggtt
    45841 gcagcgatgc cttttttgat gtcctcaaga cttgctccga gacggagaca agcaagtcca
    45901 gcagcaactg cattttcttg gttgaagttg ccaatgagtt ggatatcata atctccagcg
    45961 agtttacccg tagctgaaaa gctaaaggct ttggaattct cgatttggtt atcaaattgg
    46021 ctaccataga aatcatggtc ttgatcttca acctgttctt tcaagactga gaagtggtcc
    46081 atgtcactgt taatgatgac tgctcggcta ttttccatca agagacgctt gtggtagaaa
    46141 tagtcttcaa agctagggtg ttcaatcggg ccgatatggt ctgggctgat attgaggaaa
    46201 actcccacat caaaggttag accatagaca cgtctgacca gataggattg actggagact
    46261 tccatgatga ggtgggtacg gtcattttgc acagcctgat tcatcatgtc aaagaggtca
    46321 atactctcag gggttgtcaa cgctgactta aagaaagtct cgccatcaag agttgtgttc
    46381 atggtcgaca acatagcagg tcgatgccct tgagataaga tgttataggc gaaataggct
    46441 gctgttgtct tacccttagt accagtaaag gcaaggagtt tgagtttttc ctgtggatta
    46501 ccatagaact ccatggcaat caaactcatg gctttcttta tatcgttcac aatgatgaca
    46561 gggataccga cttcgtagtc cttttcagct acataccaag ctaagccttg tgttatagca
    46621 gaaagaaggt attctttttt aaaggcagcg ccttttgcaa aaaaaagagt gtcttctgtt
    46681 acttttcggc tatcgtagct gatgctatca aaaacaactt tgctgtagtt gtagtggtaa
    46741 tgaccttggt caataatttc gcgaaaaagg ccatctttct ttaaaatatc taatacagtt
    46801 tcaattttta tcatactttc tattgtaaac cgaaagtcgc aaatttacaa gtaacaagga
    46861 aaagtttata atggaagata aggagttttt cctagttatc aaaattgaat gaggaatcta
    46921 tgtcgcacga aaacaatcac cagcaggccc agatgttacg ggggactgct tggctaacgg
    46981 ctagtaactt tatcagtcgc ctactcgggg ctgtttacat tatcccttgg tacatctgga
    47041 tgggggctta tgcagctaag gcaaatggtc tctttaccat gggttacaat atctatgctt
    47101 ggttcttgtt ggtttcaaca gcggggattc cagttgcggt ggccaagcaa gttgccaagt
    47161 ataataccat gcgagaagaa gagcatagct ttgccctgat tcggagcttc ttaggcttta
    47221 tgacaggact aggcctggtt tttgctttag tcttgtatgt ctttgctcct tggctagcag
    47281 acttgtctgg cgtgggcaaa gacttgatcc caatcatgca aagcttggct tggggagtct
    47341 tgattttccc gtctatgagt gttatccgag gatttttcca agggatgaat aacctcaaac
    47401 cttatgccat gagccaaatt gctgagcagg tcattcgtgt tatctggatg ctcctagcaa
    47461 cctttatcat tatgaagctc ggttcaggag attatctagc agccgttacc caatcaacct
    47521 ttgctgcctt tgtcggtatg gtagccagtt ttgcagtctt gatttatttc cttgcccaag
    47581 aaggttcact caaaagagtc tttgaaacag gagataagat taacagtaag cgtctcttgg
    47641 ttgataccat taaggaagcc attcctttta tcctgacagg gtctgccatc cagctcttcc
    47701 agattttgga tcagctgacc tttatcaata gtatgagctg gtttaccaac tacagcaatg
    47761 aggacttggt tgtcatgttt tcttatttct cagccaatcc taataaaatc acgatgattt
    47821 tgatttctgt aggggtttcg attgggagtg ttggtttgcc acttttgacg gaaaactatg
    47881 tcaaggggga cttgaaagca gcttctcgtc tggttcagga cagtctcacc ctactcttta
    47941 tgttcttgct accagcaacg gttggagtgg ttatggtagg agaacctcta tatacagtct
    48001 tctatggtaa gccagatagt ttggctctgg gcttatttgt ctttgcagtt ttgcagtcta
    48061 ttattttagg cttgtacatg gtcttgtctc caatgcttca ggccatgttc cgcaaccgca
    48121 aggccgttct ctattttatc tatggttcta ttgccaagct agtcttgcaa ctacctacca
    48181 tcgccctctt ccacagttat ggtcctttga tttcaacaac cattgctctc atcattccta
    48241 acgtcttgat gtatcgggat atttgtaaag taactggtgt caagcgcaag gtgattttga
    48301 agcgaaccat tttaatcagt ttgctgaccc tagtcatgtt tctgttaata ggaaccatcc
    48361 agtggctgtt aggatttttc ttccaaccaa gtggacgttt gtggagcttc ttttatgtag
    48421 ctcttgtcgg tgccatgggg ggtggacttt atatggttat gagtctgcgt acctatttat
    48481 tagataaggt aataggaaaa gcccaagcag atcgcctgcg agcaaaattt aagctttcgt
    48541 aaggtagcat ttataaataa aaggaataag ttgaacaatc ttttaggaga acttgaaact
    48601 tattcctttt ttagcgtcaa aatagctaaa attatctttc aaaaatagaa aatgaggttt
    48661 ttttgatttt ttggaaaaaa caattgacta aacaaaccaa gagttgtata ataaaacaaa
    48721 tatttaaagc aggaggttct ggaaatgaaa aagtctaaga gcaaatatct aaccttggca
    48781 ggtcttgtcc tgggtacagg agttttattg agcgcgtgtg gaaattctag cactgcatca
    48841 aaaacctata actatgttta ttcaagtgat ccatctagtt tgaactatct agcagaaaac
    48901 cgcgcagcaa catccgatat tgttgcaaat ttggtagacg ggttattaga aaatgaccaa
    48961 tatgggaata ttattccatc attagcagag gattggactg tttctcagga cggtttgacc
    49021 tatacctaca aacttcgtaa ggatgccaag tggtttactt ctgagggaga agaatatgcg
    49081 cctgtaactg cccaggattt tgtgacaggt ttgcaatatg cagctgataa aaaatcagaa
    49141 gctttgtatc tagtgcagga ctctgttgct ggtttggatg actatatcac tggtaaaaca
    49201 agcgactttt caactgtcgg tgtcaaggca cttgatgacc aaacggttca atatactttg
    49261 gttaaaccag aactttactg gaattcaaaa acacttgcaa cgatactttt tcctgttaat
    49321 gcagatttcc tgaaatcaaa aggggatgat tttgggaagg cggatccatc tagtattttg
    49381 tacaatggac ctttcttgat gaaagcactt gtctcaaaat ctgctattga atataagaaa
    49441 aaccctaatt actgggatgc taagaatgtc tttgtagacg atgtgaaatt gacctactat
    49501 gatggtagcg accaagaatc actggaacgt aattttacag ctggtgctta tactacggct
    49561 cgtctttttc ctaacagctc cagctatgaa gggattaaag aaaaatacaa aaacaatatc
    49621 atctatagta tgcaaaattc aacttcatat ttctttaatt ttaacctaga taggaagtct
    49681 tacaattata cttctaaaac aagtgacatt gaaaagaaat cgacccagga agcagttctc
    49741 aataaaaact tccgtcaggc tatcaatttt gcttttgaca gaacatctta tggggctcag
    49801 tctgaaggga aagaaggtgc aacaaagatt ttgcgtaacc tagtggttcc tccaaacttt
    49861 gtcagtatca agggaaaaga ctttggtgaa gttgtagcct ctaagatggt caactatggt
    49921 aaggaatggc aaggtatcaa ctttgcggat ggtcaagacc cttactacaa tcctgagaaa
    49981 gccaaggcta agtttgcgga agctaagaaa gaactcgaag caaagggtgt tcaattccca
    50041 atccacttgg ataagactgt ggaagtaaca gataaagtag gcatacaagg agttagttct
    50101 atcaaacaat caattgaatc tgttttaggt tctgataatg tagtgattga cattcagcaa
    50161 ttaacatcag atgagtttga cagttcaggc tactttgctc aaacagctgc tcagaaagac
    50221 tatgatttat atcatggcgg ttggggacct gattatcaag acccgtcaac ctatctcgat
    50281 atttttaata ctaatagtgg aggagttcta caaaatcttg gactagagcc tggagaagcc
    50341 aatgacaagg ctaaggcagt tggactggat gtctatactc aaatgttgga agaagctaat
    50401 aaagagcaag atccagccaa acgttatgag aaatatgctg atattcaagc ttggttgatt
    50461 gatagttctt tagttcttcc aagtgtttcg cgtgggggaa caccatcatt gagaagaacc
    50521 gtaccatttg ctgctgccta tggtttaacc ggtacaaaag gggttgaatc atataaatac
    50581 ctcaaagtac aagataagat tgtcacaaca gacgaatatg caaaagccag agaaaaatgg
    50641 ttgaaagaaa aagaagaatc caataaaaaa gcccaagaag aattggcaaa acatgtcaaa
    50701 taaaatctaa ttgtagataa gtttgtgtaa gaagtctttg tgcttaaaaa cgcataatat
    50761 caggtatata aaatcctaga tgttatgcgt tttgttatag gaagatttat tagattttat
    50821 cctcaaattg agttattccc gactttatcc gttgaaatat tataaggagt atttatgaaa
    50881 ttaaaattat taagagtaga tactaaggtg attatgggga gtttcttact tgttctgtct
    50941 agtctacttg ctttgttgct tccccttatc ttaaagggtt taatagatgg gagttctatt
    51001 gaaaatatag gttccaaagt atttcaatcg tttttgattt ttattggtca agccttgttt
    51061 tcttctattg gttactatct gtttagtcaa tcgggtgaaa aaaagatagc aaaaatcagg
    51121 aaaaaagtga tagaggggtt g
//
LOCUS       DBERMR010000006        50800 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.006, whole genome shotgun sequence.
ACCESSION   DBERMR010000006 DBERMR010000000
VERSION     DBERMR010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 50800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50800
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.006"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(106..1467)
                     /gene="dnaA"
                     /locus_tag="ACL8A1_000428"
     CDS             complement(106..1467)
                     /gene="dnaA"
                     /locus_tag="ACL8A1_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HGS1201254.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYNYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
     gene            complement(1680..2438)
                     /locus_tag="ACL8A1_000429"
     CDS             complement(1680..2438)
                     /locus_tag="ACL8A1_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HGS1201255.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            complement(2496..3677)
                     /locus_tag="ACL8A1_000430"
     CDS             complement(2496..3677)
                     /locus_tag="ACL8A1_000430"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HGS1201256.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLRSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            3860..4339
                     /gene="rlmH"
                     /locus_tag="ACL8A1_000431"
     CDS             3860..4339
                     /gene="rlmH"
                     /locus_tag="ACL8A1_000431"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HGS1201257.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            4383..4456
                     /locus_tag="ACL8A1_000432"
     tRNA            4383..4456
                     /locus_tag="ACL8A1_000432"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4417..4419,aa:Arg,seq:tct)
     gene            4621..4746
                     /gene="comC"
                     /locus_tag="ACL8A1_000433"
     CDS             4621..4746
                     /gene="comC"
                     /locus_tag="ACL8A1_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HGS1201258.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRLSKFFRDFILQRKK"
     gene            4767..6092
                     /gene="comD"
                     /locus_tag="ACL8A1_000434"
     CDS             4767..6092
                     /gene="comD"
                     /locus_tag="ACL8A1_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence system sensor histidine kinase ComD"
                     /protein_id="HGS1201259.1"
                     /translation="MDLFGFGTVIVHFLIISHSYHFICKGQINRKELFVFGAYTLLTE
                     IVFDFPLYILYLDGLGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDEDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            6089..6841
                     /gene="comE"
                     /locus_tag="ACL8A1_000435"
     CDS             6089..6841
                     /gene="comE"
                     /locus_tag="ACL8A1_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence system response regulator
                     transcription factor ComE"
                     /protein_id="HGS1201260.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            6893..6964
                     /locus_tag="ACL8A1_000436"
     tRNA            6893..6964
                     /locus_tag="ACL8A1_000436"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6926..6928,aa:Glu,seq:ttc)
     gene            6970..7043
                     /locus_tag="ACL8A1_000437"
     tRNA            6970..7043
                     /locus_tag="ACL8A1_000437"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7004..7006,aa:Asn,seq:gtt)
     gene            complement(7084..7626)
                     /locus_tag="ACL8A1_000438"
     CDS             complement(7084..7626)
                     /locus_tag="ACL8A1_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGS1201261.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            7805..9386
                     /locus_tag="ACL8A1_000439"
                     /pseudo
     CDS             7805..9386
                     /locus_tag="ACL8A1_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518194.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
     gene            complement(9416..11968)
                     /locus_tag="ACL8A1_000440"
     CDS             complement(9416..11968)
                     /locus_tag="ACL8A1_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HGS1201262.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(12030..13652)
                     /locus_tag="ACL8A1_000441"
     CDS             complement(12030..13652)
                     /locus_tag="ACL8A1_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS1201263.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            13858..14883
                     /gene="trpS"
                     /locus_tag="ACL8A1_000442"
     CDS             13858..14883
                     /gene="trpS"
                     /locus_tag="ACL8A1_000442"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HGS1201264.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            15035..16513
                     /gene="guaB"
                     /locus_tag="ACL8A1_000443"
     CDS             15035..16513
                     /gene="guaB"
                     /locus_tag="ACL8A1_000443"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HGS1201265.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(16564..17661)
                     /gene="recF"
                     /locus_tag="ACL8A1_000444"
     CDS             complement(16564..17661)
                     /gene="recF"
                     /locus_tag="ACL8A1_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HGS1201266.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSDQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNVN"
     gene            complement(17664..18032)
                     /gene="yaaA"
                     /locus_tag="ACL8A1_000445"
     CDS             complement(17664..18032)
                     /gene="yaaA"
                     /locus_tag="ACL8A1_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HGS1201267.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            18191..19441
                     /locus_tag="ACL8A1_000446"
     CDS             18191..19441
                     /locus_tag="ACL8A1_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGS1201268.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKSWIVKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            19438..20721
                     /locus_tag="ACL8A1_000447"
     CDS             19438..20721
                     /locus_tag="ACL8A1_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGS1201269.1"
                     /translation="MTKVVFEEKYYPAVKEKVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEVTSRFHFVMLTMDTKEPVA
                     LSHQFRKSIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            20763..21584
                     /gene="rodZ"
                     /locus_tag="ACL8A1_000448"
     CDS             20763..21584
                     /gene="rodZ"
                     /locus_tag="ACL8A1_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HGS1201270.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            21595..22140
                     /gene="pgsA"
                     /locus_tag="ACL8A1_000449"
     CDS             21595..22140
                     /gene="pgsA"
                     /locus_tag="ACL8A1_000449"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HGS1201271.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            22137..22964
                     /locus_tag="ACL8A1_000450"
     CDS             22137..22964
                     /locus_tag="ACL8A1_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HGS1201272.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKFLSQNGYNLPENYLTESELEDKLWELL"
     gene            22949..23788
                     /locus_tag="ACL8A1_000451"
     CDS             22949..23788
                     /locus_tag="ACL8A1_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HGS1201273.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPSILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            23781..24575
                     /locus_tag="ACL8A1_000452"
     CDS             23781..24575
                     /locus_tag="ACL8A1_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HGS1201274.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            24634..25452
                     /gene="mreC"
                     /locus_tag="ACL8A1_000453"
     CDS             24634..25452
                     /gene="mreC"
                     /locus_tag="ACL8A1_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HGS1201275.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSITIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            25452..25946
                     /gene="mreD"
                     /locus_tag="ACL8A1_000454"
     CDS             25452..25946
                     /gene="mreD"
                     /locus_tag="ACL8A1_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HGS1201276.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            26040..27218
                     /gene="pcsB"
                     /locus_tag="ACL8A1_000455"
     CDS             26040..27218
                     /gene="pcsB"
                     /locus_tag="ACL8A1_000455"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan hydrolase PcsB"
                     /protein_id="HGS1201277.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            27442..28221
                     /gene="rpsB"
                     /locus_tag="ACL8A1_000456"
     CDS             27442..28221
                     /gene="rpsB"
                     /locus_tag="ACL8A1_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HGS1201278.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            28300..29340
                     /gene="tsf"
                     /locus_tag="ACL8A1_000457"
     CDS             28300..29340
                     /gene="tsf"
                     /locus_tag="ACL8A1_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HGS1201279.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            29587..29937
                     /gene="tnpB"
                     /locus_tag="ACL8A1_000458"
     CDS             29587..29937
                     /gene="tnpB"
                     /locus_tag="ACL8A1_000458"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGS1201280.1"
                     /translation="MIIQLSDLGQVHLVCSKIDMRQGIDSLAYVVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWLLYKRFENVRLTWPSTEKDVKALTPEQVDWLMKGFS
                     ITPKINPSESRDFY"
     gene            complement(29960..30268)
                     /locus_tag="ACL8A1_000459"
                     /pseudo
     CDS             complement(29960..30268)
                     /locus_tag="ACL8A1_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            30250..30779
                     /locus_tag="ACL8A1_000460"
                     /pseudo
     CDS             30250..30779
                     /locus_tag="ACL8A1_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_050084071.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(30854..31774)
                     /gene="cysK"
                     /locus_tag="ACL8A1_000461"
     CDS             complement(30854..31774)
                     /gene="cysK"
                     /locus_tag="ACL8A1_000461"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HGS1201281.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKPGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(32059..32694)
                     /locus_tag="ACL8A1_000462"
     CDS             complement(32059..32694)
                     /locus_tag="ACL8A1_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HGS1201282.1"
                     /translation="MEFRTIKEDDQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            32750..34048
                     /locus_tag="ACL8A1_000463"
     CDS             32750..34048
                     /locus_tag="ACL8A1_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGS1201283.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPML
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            34045..34707
                     /locus_tag="ACL8A1_000464"
     CDS             34045..34707
                     /locus_tag="ACL8A1_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HGS1201284.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQFWCKEGVGVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            34787..35335
                     /gene="raiA"
                     /locus_tag="ACL8A1_000465"
     CDS             34787..35335
                     /gene="raiA"
                     /locus_tag="ACL8A1_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HGS1201285.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNVVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            35471..37444
                     /locus_tag="ACL8A1_000466"
     CDS             35471..37444
                     /locus_tag="ACL8A1_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HGS1201286.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTSLLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            37441..37893
                     /gene="rplI"
                     /locus_tag="ACL8A1_000467"
     CDS             37441..37893
                     /gene="rplI"
                     /locus_tag="ACL8A1_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HGS1201287.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            37937..39289
                     /gene="dnaB"
                     /locus_tag="ACL8A1_000468"
     CDS             37937..39289
                     /gene="dnaB"
                     /locus_tag="ACL8A1_000468"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HGS1201288.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            39291..39557
                     /locus_tag="ACL8A1_000469"
     CDS             39291..39557
                     /locus_tag="ACL8A1_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HGS1201289.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            39646..40992
                     /gene="cbpD"
                     /locus_tag="ACL8A1_000470"
     CDS             39646..40992
                     /gene="cbpD"
                     /locus_tag="ACL8A1_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HGS1201290.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEMPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRV
                     IKANTMTGFIHFKDLDGGSVGNSQSSASTGGTHYFKTKSAIKPEPLASGTVIDYYYPG
                     EKVHYDQIFEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSATVIDYYYPGENVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSHSSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGLWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      41075..41177
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            41248..41538
                     /locus_tag="ACL8A1_000471"
     CDS             41248..41538
                     /locus_tag="ACL8A1_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HGS1201291.1"
                     /translation="MKASIALQVLPLVQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            41501..42259
                     /locus_tag="ACL8A1_000472"
     CDS             41501..42259
                     /locus_tag="ACL8A1_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1201292.1"
                     /translation="MRNLRSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     KPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRS"
     gene            42298..43305
                     /locus_tag="ACL8A1_000473"
     CDS             42298..43305
                     /locus_tag="ACL8A1_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS1201293.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            43305..44033
                     /locus_tag="ACL8A1_000474"
     CDS             43305..44033
                     /locus_tag="ACL8A1_000474"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1201294.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            44152..44610
                     /locus_tag="ACL8A1_000475"
     CDS             44152..44610
                     /locus_tag="ACL8A1_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HGS1201295.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQIIQEVDRKGK"
     gene            44612..47044
                     /locus_tag="ACL8A1_000476"
     CDS             44612..47044
                     /locus_tag="ACL8A1_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HGS1201296.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVFYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGNTLKI
                     GVKAGQLKFDIA"
     gene            47686..48339
                     /gene="rr06"
                     /locus_tag="ACL8A1_000477"
     CDS             47686..48339
                     /gene="rr06"
                     /locus_tag="ACL8A1_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HGS1201297.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDLWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            48336..49667
                     /gene="hk06"
                     /locus_tag="ACL8A1_000478"
     CDS             48336..49667
                     /gene="hk06"
                     /locus_tag="ACL8A1_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HGS1201298.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIHE
                     LESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSI
                     NKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIENQLT
                     DATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQELEH
                     LFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            49750..50277
                     /locus_tag="ACL8A1_000479"
     CDS             49750..50277
                     /locus_tag="ACL8A1_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HGS1201299.1"
                     /translation="MVLAIILVTFLIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDSMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYMTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            50356..>50800
                     /locus_tag="ACL8A1_000480"
     CDS             50356..>50800
                     /locus_tag="ACL8A1_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RICH domain-containing protein"
                     /protein_id="HGS1201300.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLFLGGVVHAEEVGGRN
                     TPTVTSSGQDISKKYADEVESHLKKILSEIQTQLDRKRHTETVALINELQGIKKTYLY
                     NLNVLKEKSKVELPSKIKAKLDVAFDQFKKDTLKPGEKVAEAQKKV"
BASE COUNT        15957 a         8220 c        10666 g        15957 t
ORIGIN      
        1 ctgtgaaatc tgtggaaaac agcctttaaa caagtcatat caagtttttt agcttgttta
       61 aaaaatgtgg ataaaaaaga taaaaaacta aaagttatcc acaaattatt tgattttctt
      121 tttgattgat tcaatttcta aacgtaaatt atcgtcttga tcaatcaaag attttatttt
      181 ggcatgggca tgaatgactg tggtatgatc ttttccccca aattccttcc caatttttgg
      241 aagactatta tctgttagtt ctctagataa atacatggct acttgacggg ccaaaacaat
      301 attttgaagg cgtctacttc ccttcatttc tttgatacta acaccataaa agttaccaac
      361 ttcagtttgg attttatcaa ttgggatgac gagcatttgg ctaacatctt gtttgcgggc
      421 tctaatggct tctgcagcaa tatcaatagt gatatccttg atttttttta ctctggcaat
      481 taaagtgatg tcgttgatgg ctccctcaag atctcgaaca tttgaatcaa attgcccagc
      541 taggtattct agagtatcac tttggaaatt gtagcctaaa tgttccgttt tactttgtaa
      601 aatggcaata cgtgtttcaa agtcaggggg tgtgatagtt tgtgtcaatc cccaactaaa
      661 acgcgtgaca agcctctcct cgagcccttc tagatgtttt ggactacgat cactcgttag
      721 gacaatctgt ttttgcttgt catgaagggc gttaaaggta ttgaaaaatt cttcctgagt
      781 tgcaactttt tttccgctga gtgactgaat atcatcgatt aacaaaagat caagactacg
      841 ataggtcttt ttaaactttt ccatttcccc aagtcttagg tgatcaagaa agtcattaat
      901 aaagctttcg gcagggatat atttaacacg cgcattagga atatttttta gaatttcatt
      961 tccaatagcg tttaataagt gagtcttacc aaggcctggt cctccataga taaaaagagg
     1021 gttataggtc agagccaaat cttcagagac agctaaagcg gctgatacag cccaaacatt
     1081 tccatcccct tgaataaagt tatcaaaggt atacttttct tttaatcccg tatctgaata
     1141 aggaatagat actaactttg gactatagtt ataaagagtt aaatttgtag cttcttcaac
     1201 ttgtgagcta gtcgtatctt gaggtttggt gaaaatatag tggggagtta tttcagcgtc
     1261 ataaatttca aaaccagcta ctacaataat atcttttagt tgtttttccc agaccatttc
     1321 catttcagag cgaggtagaa atatagtggc aacattttcc tctaccttga tgagttcagc
     1381 ttgaatagca tagaaatcat acatggatcg agtcagtctt tcttgtgcaa attctaatat
     1441 acgattccaa aattgttttt ctttcaatcc tttctcctcc tttactattt aaaagtttaa
     1501 tactagagat attttaccat agatagcaag aattttccac aagctgtgaa aaataatcca
     1561 caatgttacc aaactttatc cacaggttgg ggataaaaga agaaattatt gatttattaa
     1621 gctttttatc gaaaaacata gtggataacc ttgtgagata aaaaaataaa agaacagcct
     1681 tatttcaggc tgttgataat tctactatat tcttcttgat ttgaaaaaga aataatgatt
     1741 tttccactgt cttttttaga tagtttaatt tctacatcta atccgagtag ttttcttaac
     1801 tgtttttctt cattttgtat gaaatgatta gttttttgct gtttcttttg ttttttctct
     1861 gtcagaagag cttctaattt ccttacagaa atatcttctt ctataatccg ttgaaagaaa
     1921 tagtcttgtt gttccttatt taacccaact agggaacgcg catgggcttg tgatagtttg
     1981 ccattttcta cttctgaaag aatctgttct ggcaaggaaa gtaaacgaat ggagttgctg
     2041 atatatggac gagacttgcc catcttatct gcaatttcag catgggtgaa tcctttctct
     2101 acgagagatt cataggcgcg tgcttcttct attgggttta aattttctct ctgtaaattt
     2161 tcaataatgg actggaccat catctcttgg tctgaaatct gtttaacaac agctgggata
     2221 gaccgtagac cagctaaaag tgaagcccga tagcgtctct ctcctgcaag gatttcataa
     2281 ccaataacag gagattgacg aacaataatc ggttgaatga ccccattttc tttgatagac
     2341 tgtgctagtt catctagttt ttctctatca aattcttttc ggggttgata gggatttttt
     2401 tgtatatctg tgatagaaat catttcaaat ttttccatga ttctacacta acacatcttt
     2461 tctcttatgt aaagctttct ttacatagat gtcaatcaag attctaaatc acctgaactc
     2521 ttgttaagtt tgatagaggt agtttcttct ttcccgttac gatagtaggt tatcttaatg
     2581 gtgtctccga tagaatggtt gtaaagagca ctttgtaagt ctgttgatga agcaatctct
     2641 ttgtcatcta cttttgtaat tacatcgtat ttttcaaggt gaccattggc aggcatatta
     2701 ctttgtaccg aacgaacaac tacaccagat gtaacattac ttggaatatt gagtcttctg
     2761 atgtcgcttg tactcacatt agataaatta accatctgga ttcccaaagc tggacgcgtc
     2821 acttttccgt ttttttctaa ctgttcaata atattgatag catcatttgc aggaattgcg
     2881 aaaccaagac cttctacaga tgttcctcca tttgtagcaa ttttacttga ggtaattccg
     2941 ataacctgcc cttgaatatt gatcagtggg ccgccagagt tacctgggtt aatagcagta
     3001 tcagtttgga tggcttttgt agaaatagct tgtccatctt ccgatcttaa ggatacattt
     3061 ctattgagac tggatacgat accttgagtg acagtatttg catattcaga acctaacggg
     3121 ctaccgatgg caatagcagt ttctcctaca gttaacttac tagaatcacc aaactcagct
     3181 actgttgtca ctttttctga agagattttg acgacagcaa tatcagagaa agtgtcagct
     3241 ccgacaattt ctccaggtac tttagtccca tctgacaatc taatatctac tttgctggcg
     3301 ccatttataa cgtgattgtt ggtgacgatg taagcttctt tatcattctt tttataaata
     3361 actccagatc cttcactaga gattcgctga gaatctgtgt cagtatcatc attgccaaat
     3421 acgctatttt gtctgtttgc cgaataagta ataacagaaa caacagcatc ttttactttg
     3481 ttaacagcct gtgttgttga attttcgttc ttataggcag tttgtgtaat agtactattg
     3541 ttgttagagt tgtttacact acttttttga gttagttgag ttattgaaaa actacccaag
     3601 gctccactaa aaaagctaat gacgataacg actaataatt gaaaccattt tttgtaaaat
     3661 gtttttagat gtttcatatt tgcctccata tgtttgaatt actgaaagta taaactgact
     3721 agcttaatta taacttaaac acaaaagttt tacacaaact gtggataact cttttgaaac
     3781 tgtgattttc ttaattgaaa tctatttttt attttgtgaa taagatgtga aaaaatagag
     3841 aatatgttag aatagagtca tgaaaattaa agttgtaaca gttgggaaac tgaaagaaaa
     3901 gtatttaaaa gatggtatcg cagagtattc aaaacgaatt tctagatttg ctaagtttga
     3961 aatgattgag ttatcagatg aaaaaacacc agataaggcc agtgaatcag aaaatcaaaa
     4021 gattttagaa atagaaggtc agagaatttt atcaaaaatt gctgaccgtg atttcgttat
     4081 tgtgttagcc attgaaggga aaactttctt ctcagaagaa tttagtaagc agttagaaga
     4141 aacttctata aaaggatttt ctactcttac ttttattatt gggggaagtt taggattgtc
     4201 atcatctgta aaaaatagag ccaatctttc tgtcagtttt ggtcgcctaa ccttacccca
     4261 tcagttaatg agactagttc ttgttgaaca aatctatcgc gcttttacga ttcagcaggg
     4321 attcccctac cataaataga aaattgactt ttaattgaat ttttggtaga ataattgtat
     4381 taggtctcat agctcagctg gatagagcat tcgccttcta agcgaacggt cgcaggttcg
     4441 aatcctgctg ggatcaatat aatagcaaag ctgggaattt tcccggcttt tttcttaaaa
     4501 aagtacactt tgggagaaaa aaatgacagt tgagagaatt ttatctaaaa cgaaattcca
     4561 ttttgtataa tggtttttgt aagttagctt acaagaaaaa acattttagg agattttatt
     4621 atgaaaaaca cagttaaatt ggaacagttt gtagctttga aggaaaaaga cttacaaaag
     4681 attaaaggtg gggagatgag gttgtcaaaa ttcttccgtg attttatttt acaaagaaaa
     4741 aagtaatgaa ataaggggaa agagtaatgg atttatttgg atttgggacg gttattgttc
     4801 attttttaat tattagtcac agttaccatt ttatttgtaa aggtcaaata aatagaaaag
     4861 aattattcgt ttttggtgct tatacattac taacagaaat agtatttgat tttcccttat
     4921 atattctata tttagatggg ttagggattg aaagattttt atttcctttg ggattatatt
     4981 cctattttcg atggatgaaa cagtatgaga gggatagagg actattccta agtttactac
     5041 tatctctttt atatgagagc actcataact ttctgtccgt aactttctcc tctataacag
     5101 gagataattt tgttttacaa tatcatttcc cattcttttt cgttgtgacg gtgttaacct
     5161 attttgttac attaaaaatc atttactatt tccatttgga actagcctat tttgacgagg
     5221 actaccttta tcctttcttg aaaaaagtat tttttgcttt actattgcta catattgtat
     5281 ctttcgtttc agatatggta agtacgatta aacatttgaa tagttttgga agtattttgt
     5341 catctattgt ctttatctct ctccttttga ccttctttgc aatgaattct cataaagttc
     5401 aaatggagaa agagattgct ttgaagcaga agaaatttga acagaaacat ttacagaatt
     5461 acacagatga aattgttggt ctgtataatg aaatccgtgg ttttcgacat gattatgctg
     5521 gaatgcttgt cagcatgcag atggcaattg acagtggtaa tttacaggaa attgacagaa
     5581 tttacaatga agttttagtc aaagcaaatc ataaattgcg ttcagataag tacacttact
     5641 ttgatttgaa caacatagaa gactcagctt tacgaagttt ggttgctcag tcaattgtct
     5701 atgctcgaaa taatggtgta gagtttacac tggaagtaaa agatacgatt accaagcttc
     5761 caattgaact attggatttg gttcgtatca tgagcgtttt attgaataat gctgtcgaag
     5821 gatcggctga tagctataaa aagcagatgg aagtagcagt tattaagatg gaaactgaaa
     5881 cagttattgt gattcagaat tcatgtaaaa tgacgatgac tccttcagga gatctatttg
     5941 ccttaggatt ctccactaag ggaagaaatc gcggagtcgg attaaataat gtgaaagaac
     6001 tactagataa gtacaacaat attattttag aaacagagat ggaaggcagt acatttagac
     6061 aaatcattag atttaagagg gaatttgaat gaaagtttta attttagaag atgttattga
     6121 acatcaagtg agactagaga gaatattgga tgaaatttcg aaagaatcga atattccaat
     6181 atcatacaag acaacgggaa aagtccgtga atttgaagaa tacattgaaa atgatgaagt
     6241 aaatcagctt tatttcctag atatcgatat tcatggaatt gagaaaaagg gatttgaagt
     6301 ggctcagctc attcgtcatt acaatcctta cgctattatc gtctttatca ctagtcgatc
     6361 agagtttgcg actctaacct ataaatacca ggtatcagcc ctagattttg ttgataagga
     6421 tatcaatgat gagatgttta agaagagaat tgagcaaaat atcttctaca cgaagagtat
     6481 gttacttgaa aatgaagatg ttgtagatta tttcgactac aattacaagg gaaatgattt
     6541 aaaaattcct taccatgata ttttgtatat tgaaacaaca ggggtatctc ataaattgcg
     6601 cattattggt aagaattttg caaaagagtt ttatggtacc atgacagata ttcaggaaaa
     6661 ggacaaacat actcagcgat tttattctcc tcacaagtca tttttggtaa atataggcaa
     6721 tatcagagaa attgatcgaa aaaacttaga aattgttttc tatgaagacc atcgttgtcc
     6781 tatttcaaga ttaaaaatta gaaaattaaa agatatttta gagaaaaaat ctcaaaagtg
     6841 attgacaatt agcaagaaat tgatataatg gttatatctg ctacagatag aaggtccgtt
     6901 ggtcaagggg ttaagacacc gccttttcac ggcggtaaca cgggttcgaa tcccgtacgg
     6961 actattttat ccgccatagc tcagttggta gtagcgcatg actgttaatc atgatgtcgt
     7021 aggttcgagt cctactggcg gagtatagat aaaagggaac acagctgtgt tcctcttttt
     7081 gtatcaattt gtatcaccaa gcattttcat aaggaagtct gttatttctt gagaactttc
     7141 tttttttcca tgtgcaatcc aagtttggca gacaccaaaa agtgcatgag ttagatagat
     7201 gctactatat tctaattcag tggtatttag attcagttgc ataaatcgct tttgtaaatc
     7261 tgtactaagc atgatatgaa gtttatttcg taagaaattt tggatttctt tagtcccatt
     7321 ttcagaaaga agggcagcca gaagtggttc tgactctaga tattcaaaaa cttctaaaat
     7381 agcgtctctt ttgtgatgag catgtttttg aaaaatatat tcaaatgtat ggaatagctt
     7441 gctttgatag tgctcaatca tatcatactt atccttatag tgagtataga agctggaacg
     7501 actaattccg gctttttcta ctaatttgac agtagaaatt ttatcaaatg gctgttccat
     7561 cagtaattgt accatagcat tttcaatagt tcgctttgtt tttaagcgtt tgttactttc
     7621 ttgcatattt cctccttgta aacaaattag actatatgtc taaaaataga ttttttatct
     7681 tgtaatttag attttttaat gtataatcta ttatatcaaa attttagaca atatgtttaa
     7741 aaaaggagaa actatgttta aagaatggaa agcaatttaa aaaaaaacaa cctttattat
     7801 tgtcatgatc gggatttctc ttattccagc tctgtacaat atcatatttt tgtcatcaat
     7861 gtgggatcca tatgggcaat tgtctgactt acctgtggca gttgtaaata atgataaaga
     7921 ggcttcctat aatggtaata ctatggcaat aggaaaagac atggtgtcca atttaaaaga
     7981 aaataaaacc ttggattttc attttgtaga tgaagaggaa ggaaagaagg gattggaaga
     8041 tggcgattac tatatggtag tgactttacc aagtgattta tctaaaaaaa caactacatt
     8101 atccaatatt caatcgacag cagcttatca atcattgaca agtgagcaac aaactgggat
     8161 aagtgattct gtatctcaaa attcaactga tagtattcaa tcggctcagt caattgtagc
     8221 tttagtacaa ggtttacagg gagtttagaa aacttacaaa atcaatcttc taatctttcg
     8281 actttaaaaa atcaagctaa tcaagtatta cctattactt ctacttcttt gataggattg
     8341 tcaagtggat taacagagat acaaggagct gttactagca aattagttcc tgccagtcag
     8401 ttgattgcat caggtgtaaa cgcatatact acaggtgttg ataaagtttc tcagggcgca
     8461 agtcaactaa gtgaaaaaaa tgccaccttg acaggtagtt tggataaact agtttcaggc
     8521 tcaaacacct tgacacaaaa atcttctaga ttgacagcag gatctgggca attagcagac
     8581 aaatccagtc agttactttc aggtgcttct ccattagaga atagagctaa taaattggca
     8641 gatggatctg ggaaactagc agaaggtgga acaaagttaa cttctggatt ggaagattta
     8701 cagacaggac ttgcttcttt aggacaagga ctaggtaatg ctagtgatca actcaaatca
     8761 gtatcaacag aatctaaaaa tgcagagatt ttgtcaaatc cactcaatct ttcaaaaaca
     8821 gacaatgatc aagttcctgt aaatggaatc gcaatagctc cttatatgat atcagttgct
     8881 cttttttttg cagcaatatc aacaaatatg atatttgcga aattgccttc aggacgtcat
     8941 ccagagagcc gttgggcttg gttgaaatct tgagctgaaa taaatggtat tatagctgtt
     9001 ttggcaggaa ttttggtata tggaggagtt cagcttattg gtttaactgc taatcatgag
     9061 atgagaatat ttattctcat catcctaaca agtttagtat tcatgtctat ggtgaccact
     9121 ttagcaacgt ggaatagccg tataggagct tttttctcac ttattttgct tttactacag
     9181 ttagcatcaa gtgcaggtac ttatccactt gctttgacaa atgatttctt tagatctatt
     9241 aatccctggt taccaatgag ctattcagtt tcgggattac gacaaacaat ctctatcaac
     9301 aagtcatttt cctagctgtc atactagctc tatttactgg tttaggtatg ctagcctatc
     9361 aacataagaa aatggaagaa gattaaaaaa atcgaccgat taactggtcg attttttatg
     9421 ccttagatga ctttcgtctg tgattataga ttccaaatag taagagagaa gtaaaggaac
     9481 agattgctcc agtaataaaa ccattgggaa tgaaggaaag tgtaatagtt cctttcccct
     9541 tgggaatgtc aactttcata aatccagttt gagcttgttt aatttctatt ttcttaccat
     9601 cttggtaggc agaccaacct ttgtcataag gaatggtgaa gaaaatagat gtatcttgtt
     9661 ggacatcata tgtagcaaaa accttgtttt tagaagttga tactgtgaca ggttgttctt
     9721 taattttttg aattgcctcg gtgaaagttt tggtatctaa acgatagaag gtaggagatt
     9781 caaatgatac ttgtgaattt ccagggaaac taacattgat attgaaagtt tttttctctt
     9841 tagtatatcc tagattaaag aaggagaaga cattatcagt tgtaaaagtc tttttttcac
     9901 catttacaag tatgtcaacc ttcttttgtt tatcgttaga aaagtgaagg tttatgaaag
     9961 agagataaac ttggctgttt tctggaactt caatttgata ctggattgct gcatcttcat
    10021 ttgaagaact tgtgacacta atcaaatcat tagtattttc tattttttct gttttttcat
    10081 aaggtattgg agaaaaataa tcaaaattga cgttagcaag ttgatttaaa aatgaggcct
    10141 gattatccaa ggtatgttca ttgaacttga catcattgta aacagattga ctagcaactg
    10201 caatcggaag agagtattga ttttcatata gggtaagatt atctttttga tagatatctt
    10261 taaagccata cttatcaata ggactgtctg agatattgta ctggatacca aataaactat
    10321 cagccaaaat actattattt gcatatcgga gattgagatt agtcccagag gatttaaaac
    10381 caagtttatc taaagtagag cttgatgaac gatttcgaac agatgaaaat tgagagattc
    10441 cattgtagtt gaatttcata ctgtcatttc ctgtctgagt ttgtagtttt tcagtacgag
    10501 taaattgatt tccaatatat gttgagaaag attccatagc tgggatatct cgactataag
    10561 cacttcgaga agcaaatccc cattccttag caattccgtc catttgagat gaagcattta
    10621 aactcatttc aaccagtata aataaagaga ttagaatggc aaatagattc acagatataa
    10681 actttttgat aactgcaagg agtaaaagag aatagacaac caaaaattca agagtaagca
    10741 gaatattcaa atctgttaaa aaagaataat gcgattttag atagatggta gctaaaaatc
    10801 ctgctactac aagaaaaagc gaaactaaaa aattccagac tttaagttct ttcagacgct
    10861 ttaagacttc tgctgctgtg taaattaaca aggtagagaa aatccaagca taacgatgta
    10921 aaaacatgtt tggagtatgc atgccttgcc aaaataagtc aagagcttct atgtaaaagc
    10981 ttgcaattag aaatgcaaag aatattacat atatgagttt cacgtgaaac ttaatagatt
    11041 tcagcgtaaa aaataaaatg gtcaaaataa agggaaatag tccaacaaaa atcattggga
    11101 tggccccata ctttgttgtg tcaaaggaac caatgaattg cttagcaaag agatcaagat
    11161 accagctact ttcagtttga aactttgtaa cttcagtcaa tttttcccca tgtgtctgta
    11221 aatcaaatag agtgggaaga gtcataatca aactagccat accagctaaa aaggagataa
    11281 ctatgaaatc aagaacagat gattttcgag tcttaaagtc ccacgaaatt tgacagagat
    11341 accagaaaat aagaaacaat actgtcatat atccaaaata ataattttga ataaataaga
    11401 ttgacagact tgtaaagtac aataggagtt tcttttcagt tatcagtaga tgtaaaccag
    11461 ttataattaa aggaatcaag ataaaaacat ctagccaggt ttttatctct aattgactga
    11521 cagtgaaact catcagagca taggaagtag ataaggctag ttttaaagtc tgagggatag
    11581 attgaaataa tttattcaaa ctaaaaaagg ttgacagacc aatcaatcca aattttaaga
    11641 gagtggtcag atagacagca tctggcatat tcgttagatc aaaaaagtaa actagaggcg
    11701 cgagaaaact acccaagtaa taactagata gggcatagaa gtttagccct agaccacttg
    11761 taaaggtgta aaacagacta ctatttccat gtaggatatt tcgtaaggct acatcaaaaa
    11821 taacgtattg atgaaagcca tctcctaata gaggagagtt gtcgctattc cagtagatac
    11881 cttgagatag atatactcca gacataatca ctacaggaat gatgaaagaa ataaaatagg
    11941 ttcgatatgt ttttaaaaat gatttcatgt tacctcgtag aatgatagaa aactcagttg
    12001 gttaacccaa ctgagttttg aagttttatt tagtctttcc aaagttcttt aacttttgct
    12061 tgtacttctg cattttctag gaattcatcg taggtttcat cgatacggtc aatgacgcca
    12121 tttttagata agacaatgat atggttagcc aaagtttgaa taaattcgtg gtcatggcta
    12181 gcaaagatga ttgattcttt aaagtttttc aatccatcat tcaagcttga gatagattcc
    12241 aagtccaagt gatttgttgg atcatcaagt acaaggacat ttgattttaa gagcatgagt
    12301 tttgaaagca tgacacgaac tttttctccc cctgacaaga catttacagg tttgttaact
    12361 tcatctccag agaagagcat acggccgagg aagccacgta ggaaagtatt gtcatcttct
    12421 tctttacttg cgaattgacg caaccagtca agaattgatt ctcctcctgc aaaatcagct
    12481 gagttatctt ttggtaggta agattgacta gttgtaactc cccacttgac agttccttca
    12541 tagtcaatat ctcccatgat tgcacgaatt aatgcagtcg tttgaatatc attttgtcca
    12601 ataagtgctg tcttatcatc tggacgcaag atgaaactaa tattatccaa gatagtttca
    12661 ccatcaatct ttacagttag attttctact gtcaagagat cattaccaat ctcacgttcc
    12721 gctttaaagt tgataaatgg atatttacga ctagatggca caatctcttc tagctcaatc
    12781 ttatcaagca ttttcttacg tgatgttgcc tgccttgact tagaagcatt ggcagagaaa
    12841 cgagcaacga attcttgcaa ttgtttaatt ttttcttctg ctttagcatt acggtctgct
    12901 agcaatttag cagcaagctc agaagattcc ttccagaagt cgtagtttcc gacatagagt
    12961 ttgatttttc caaagtcaag gtcggccatg tgagtacaaa ctttgtttaa gaagtgacgg
    13021 tcgtgggata ctacgataac tgtgttatca aagtcaatta agaagtcttc taaccaagta
    13081 atcgattgga tatccaaacc gttagtaggc tcgtccaaga gaagaacatc tggtttacca
    13141 aaaagtgctt tggcgaggag aacctttact ttttcaccgt tggccaattc gctcatgttt
    13201 tggtagtgta attcttctgg aatgtttagg ttttgaagta gttgagaggc ttcactctct
    13261 gcttcccaac ctccaagttc ggcaaactct ccttcgagtt cggcagcacg aaccccgtcc
    13321 tcgtctgaga aatcttcctt catgtagata gcatctttct ctttcatgat gctataaagt
    13381 ttttcatttc ccatgataac gacatcaatg gcacgttcat cttcgtagtc aaagtgattt
    13441 tgacgaagaa cagagagacg ttcatctgga ccaagagaga tgtgaccagt agtaggttcg
    13501 atatctccag ctaaaatttt taaaaaggtt gattttccgg caccattagc accgattaat
    13561 ccgtaagtat ttccttctgt aaatttgata ttgacatcat caaaaagttt gcgatcacta
    13621 aaacgtagtg aaacatcaga tactgtaagc aatatttttc tcctatatgt gtaatatatt
    13681 tattctacta gaaaatacag aaatattcaa atttttattt gtcaattttg tgtaaattat
    13741 atttacagta tcctttacac aaatctgtaa aaagcaaggc tgatttattt tgataaatta
    13801 cggttatttc attaaaaaaa tgctataatt gaaaggacta tatcgaagga gaacaaaatg
    13861 actaaaccca ttattttaac aggagaccgt ccaacaggaa aattgcatat tggacattat
    13921 gttggaagtc tcaaaaatcg agtattatta caggaagagg ataagtatga tatgtttgtg
    13981 ttcttggctg accaacaagc cttgacagat catgccaaag atcctcaaac cattgtagag
    14041 tctatcggaa atgtggcttt ggattatctt gcagttggat tggatccaaa taagtcaact
    14101 atttttattc aaagccagat tccagagttg gctgagttgt ctatgtatta tatgaatcta
    14161 gtttcgttag cacgtttgga gcgaaatcca acagtcaaga cagagatttc tcagaaagga
    14221 tttggagaaa gcattccgac aggattcttg gtctatccaa tcgctcaagc agctgatatc
    14281 acagctttca aggctaatta tgttcctgtt gggacagatc agaaaccaat gattgagcaa
    14341 actcgtgaaa ttgttcgttc ttttaacaat gcatataact gtgatgtctt ggtagagccg
    14401 gaaggtattt atccagaaaa tgagagagca gggcgtttgc ctggtttaga tggaaatgct
    14461 aaaatgtcta aatcactaaa taatggtatt tatttagctg atgatgcgga tactttgcgt
    14521 aaaaaagtaa tgagtatgta tacagatcca gatcatatcc gcgttgagga tccaggtaag
    14581 attgagggaa atatggtttt ccattatcta gatgtttttg gtcgtccaga agatgctcaa
    14641 gaaattgctg atatgaaaga acgttatcaa cgaggtggtc ttggtgatgt gaagaccaag
    14701 cgttatctac ttgaaatatt agaacgtgaa ctgggtccta ttcgtgagcg ccgtattgaa
    14761 tttgctaagg atatgggaga agtttataat atgattcaaa aaggtagtga aagagcgcgt
    14821 gaagttgcgg gtcaaaccct atctgaggta aaaggggcaa tgggacttca ttactttaac
    14881 taagtttatt ttacaagaaa ctatcatttc tatctcgaag aaaagatagt tttttattta
    14941 cccctgtaac atttgacaaa tttgtataga atggtatgat agatacaata taaaaagagt
    15001 caagctcaaa aagaaagaaa agaggaaact tcgaatgtct aattgggaca ctaaattttt
    15061 gaaaaaaggt tttacctttg atgatgtatt gcttattcca gctgaaagtc atgtgttgcc
    15121 taacgatgca gatttaacaa ctaaattggc agataatttg actttaaata tcccaattat
    15181 taccgctgcc atggacacag ttacagagag tcaaatggcc attgctattg ctcgtgcagg
    15241 tggtctcgga gttatccata aaaacatgtc aattgctcaa caagcagacg aggttcgtaa
    15301 ggtaaaacgt tctgaaaatg gagttattat tgatccgttc ttcttgacgc ctgaacatac
    15361 aattgctgaa gcagatgagc ttatgggtcg ttaccgcatc agtggtgttc cagttgttga
    15421 aacacttgaa aatcgtaaat tggttggtat tttgacaaac cgagatcttc gttttatttc
    15481 agattataat caaccaattt caaaccatat gactagtgaa aatcttgtta ctgctcctgt
    15541 gggtacggat cttgcaacgg ctgagagtat tcttcaagag catcgtattg aaaaacttcc
    15601 gttggtcgat gaagaaggca gtctttctgg tttgatcact atcaaagata ttgaaaaagt
    15661 tattgagttt ccaaatgcgg ctaaagatga gtttggtcgt cttctagttg caggtgcagt
    15721 aggtgttact tcagatacat ttgaacgtgc agaggctctt tttgaggcag gagcggatgc
    15781 gattgttatt gatactgcac atggtcattc tgcaggtgtc ttgcgtaaaa ttgccgagat
    15841 tcgtgctcat ttcccagatc ggactttgat tgctggaaat attgctactg ctgaaggtgc
    15901 acgtgccctt tatgaagcgg gtgtagacgt tgttaaggtt ggtattggac caggttctat
    15961 ctgtactact cgtgtgattg ctggtgttgg tgttccgcaa gtaacagcta tctacgatgc
    16021 tgcagctgtt gcgcgcgaat atggtaaaac gattattgct gacggtggga tcaagtattc
    16081 tggagatatt gtaaaagcac ttgctgcagg tggaaatgct gttatgcttg gatctatgtt
    16141 tgctggaact gatgaagctc caggcgaaac tgaaatcttc caaggacgta aattcaagac
    16201 ttaccgtggt atgggatcaa ttgctgctat gaagaaaggt tcaagcgacc gttatttcca
    16261 aggttctgtc aatgaagcaa acaagcttgt tccagaagga attgaaggtc gtgttgctta
    16321 taaaggagcg gcagctgata ttgttttcca aatgattggt ggtattcgct ctggtatggg
    16381 ttactgtggt gcagctaacc ttaaagaact acacgataat gctcaattta ttgaaatgtc
    16441 tggtgctggt ttgaaagaaa gccatcctca tgatgtgcaa attactaatg aggcaccaaa
    16501 ttattctatg taaaaaacaa tgaaaagaac tccagtgaaa acaggagttc ttttacaatg
    16561 ttgtcaattt acatttacag cagctttacc atcctgaata gtgaagatac ttagattttc
    16621 tggcagattt tgaagatggt ctaagcttgt tgttgtgata aaggtttgga ttgattgaga
    16681 aatcgtttct aataatttta actgtctagt gttgtcaagt tcactcatca catcgtcaag
    16741 cagtaatatc ggagattctg tggtaatgct ttccattaat tcgatttctg ctaattttat
    16801 cgagaggacg agactacgat gttgaccttg gcttccgaaa ctagcatcca tcccatttat
    16861 ataaaaagaa atgtcatctc gatgaggacc gacaccagta ttctttttaa ataaatctct
    16921 ggatctactt ttttctaaag caattttgaa agattcggat aagttttgtt tgtcagttat
    16981 attgacagaa gattgatagg atattgacaa ctcttcgatc tgattagaga gttcaaaatg
    17041 tttcttacgc ccaaatgatt ctagtttttt tatgaaatct aagcggtgat tcattacacg
    17101 acatccataa tcaactagct gatcatctaa cacagaaagg aatgtttcat ctattttttg
    17161 atctgatttt aggtaagtgt ttctttgctt taggatgtgg ttataattgg ttaagtcaga
    17221 taagtagatt ggcttaattt gcccaagttc catatcaatg aattttcgtc gaatcgaagg
    17281 tgctccttta attagttgta aatcttcagg agcaaataag acaacattca tgtgtcctac
    17341 ataatctgaa aggcgtgcct gttttaagtg attaactttt gttacacggc ctttttgtgt
    17401 tagttcgatt tctaggggaa tggatccagt ttttttctga acgagacctg aaagatgaag
    17461 ttgttcctca tcaaaatgaa tgagattttt atctgttcga gttcgatgac tacgcgttaa
    17521 ggctaaaaaa tagatagcct ctaacatgtt tgtttttcct tgtgcattac gtcccaaaaa
    17581 gacattcaat ttaggattaa agtctatttt cgtttctttg taattacgaa aagtcttgag
    17641 agatagatgt tgtagccaca tgattatcta ccagggaatc gtggagcttg ttggctttta
    17701 ggtgatgaag caggtttcga tttgtctttt ttaactccct tattcatctt tttgacaagt
    17761 ttagcgatcc gttctttttc aactttatca gcttggtatt catcttgttc ttcagaagta
    17821 ggttgtgtca acaagatgtc aatgttcatg tcagggatgt caactttatc gccaatacga
    17881 agtttttttc cacgacgact ctctaattcc ctattaaagt aaacagaatg ttcagagaga
    17941 aatgatttaa tagctcctcc gctatgtgta attccaagtt ctttgagtag tgcttggagg
    18001 gtaataaatt cttcaaataa tttgtattcc ataattcacc tcaatctttg gtattatacc
    18061 atattttcct aaaaatagtg agagagacag agagaaatgt aagcgatttt tatttcaaaa
    18121 gtgttacagg gaataagaaa atttcaggtt ttcgtgatat aatagaagtc tgtatataag
    18181 gaggtaaatc atggagttag tgcatggaat ttcaacacat tttatccaat caaaaaagtt
    18241 taaaacaaac aaaattaccg tgcgttttac cgctccatta tcccttgata cgattgcagg
    18301 tcacatgttg agtgcaagta tgctagagac tgctaatcag atgtacccca cttctcaaga
    18361 tttgaggaga cacttggcca gtctatacgg tacagatatg tcaaccaatt gtttcagaag
    18421 agggcaaagc cacattatag aattgacatt tacctatgtt cgtgatgagt ttttaagtag
    18481 gaaaaacgtg ctaacctctc agattttgga acttgtaaaa gaaactcttt tttcacccgt
    18541 agtagttgat aatgggtttg atccggcctt atttgaaatt gagaaaaaac aattgctagc
    18601 aagtttagca gctgatatgg atgattcttt ttattttgca cataaagaat tggataaatt
    18661 gttttttcat gatgaacgtc ttcaattgga atatagtgat ttacgaaatc gtattttagc
    18721 tgaaactcca caaagttctt attcttgttt ccaagaattt ttagccaatg atcgaataga
    18781 tttctttttc ctaggtgatt ttaatgaggt tgaaattcaa aatgtattag aatcatttgg
    18841 ctttaaaggt cgaaaaggag atgtgaaggt tcagtattgt caaccttatt ctaatatcct
    18901 tcaggaaggt atggttcgga aaaatgtggg acaatccatt ttggaattag gttatcatta
    18961 ccgttctaaa tatggtgatg agcaacattt acccatgatt gtaatgaatg gtttacttgg
    19021 tggatttgct cattctaagc tctttacaaa tgtccgtgaa aatgctggat tagcttatac
    19081 catttcaagt gagcttgatt tatttagtgg attcttgagg atgtatgctg gtatcaatcg
    19141 agaaaatcgt aaccaggctc gtaaaatgat gaataatcaa ctgcttgatt taaaaaaagg
    19201 ttattttaca gagtttgagt taaatcagac caaggaaatg attcgttggt cgttgttact
    19261 ttctcaagat aatcaatctt cattgattga acgtgcttat caaaatgcct tatttggaaa
    19321 atcttcagca gactttaaaa gttggattgt aaagcttgaa cagattgaca aagatgctat
    19381 ttgtagagta gctaataatg tgaaactaca agcgatttac tttatggaag gaatagaatg
    19441 acaaaggttg tttttgaaga aaaatactat ccagctgtaa aagaaaaggt ttatcgaact
    19501 cgtttggcca atggattgac agttgctctt ttgcctaaaa aggaatttaa agaggtttac
    19561 gggagtgtca ctgtacagtt tggttcggta gatacgtttg tcacagaagt tgacggagat
    19621 gtaaaacaat atcctggagg aattgctcat tttcttgaac ataaattatt tgagagagaa
    19681 gattctagtg atttgatgtc ggcttttacg agtctaggtg cagatagtaa tgcctttaca
    19741 agctttacaa aaacaaacta tcttttttca gcaacggatt attttttaga aaatttagat
    19801 ttacttgatg aattggtaac atcagcacac tttactgaag cttccattct gacagagcag
    19861 gatattattc agcaagaacg agaaatgtac caagatgatc cagattcgtg tttattcttt
    19921 tcaactttag cgaatttgta tcctggtaca cctttagcaa ctgatatagt tggaagtgag
    19981 gagtccattt cccaaatcaa tctaactaat ttgcaagaaa attttacaaa gttttacaaa
    20041 cctgtaaaca tgtctctgtt tttagttggt aattttgatg tggagcgagt acaggactat
    20101 tttgaaagca aagaactgaa agattcagat tttcaggaag tagcaagaga aaagttgttt
    20161 ttacagcctg taaagccaac agatagtatg agaatggaag tatcttctcc caaactagcg
    20221 attggagtta gaggtaagcg agaagtttct gaagcggatt gctatcgaca tcatatttta
    20281 ttaaagttat tgtttgcaat gatgtttggt tggacttcgg atcgttttca aaaatgttat
    20341 gaatcaggta aaattgatgc atccttatct ctggaagttg aagtaacaag tcgctttcat
    20401 tttgtcatgt tgacaatgga tacgaaagag ccagttgctt tgtctcatca atttaggaag
    20461 tctattcgta attttacaaa ggatttagat attacagagg aacatttaga tattatcaaa
    20521 agagagatgt ttggcgaatt tttcagtagc atgaactctc ttgaatttat tgcaacgcaa
    20581 tatgatgctt ttgaaaatgg tgagacaatt tttgatttgc cgaaaatttt acaggaaatt
    20641 actttagagg atgtccttga tgctggacat catttaatag atgatggtga catagttgat
    20701 tttacaatat tcccatcgta gtaacctatt ataatagaca ctagaaagaa gggatgacaa
    20761 gtatgagaaa aaaaacaatt ggagaggttt tacgattagc tagaatcaat cagggattga
    20821 gtttagatga attgcagaaa aagacagaaa tccagttaga tatgttggaa gcaatggaag
    20881 cagacgattt cgatcaactt ccaagtcctt tttacacgcg ttctttcttg aaaaaatatg
    20941 catgggctgt tgagttagat gaccaaattg ttttggatgc ttatgattct gggagtatga
    21001 ttacttatga ggaagtagat gttgatgaag atgagttgac aggtcgtaga cgttcaagta
    21061 agaaaaagaa gaaaaaaaca tcatttttac ctttatttta ttttatcctt tttgctttat
    21121 cgattttaat ttttgtgact tattatgttt ggaactatat tcaaactcaa ccagaggagc
    21181 cttctctttc taattacagt gtggttcaat caacaagttc aactagctct gttccccact
    21241 cctcaagtag tagttcttct agtatagaat cagctataag tgtatcaggc gaaggaaatc
    21301 atgtagaaat cgcttataag acaagtaagg aaacagttaa attgcaattg gcagtttcag
    21361 atgttacaag ttgggtcagt gtttcagaaa gcgaacttga gggcggtgta accttatcgc
    21421 caaagaagaa aagtgcagaa gcaacagttg caactaaaag tcctgtaaca attacgttag
    21481 gtgttgtaaa aggtgttgat ttgacagtag ataatcagac tgttgattta tcgaaattaa
    21541 cagctcagac tggacaaatc actgtaacct ttactaaaaa ttaaggaaaa acgaatgaaa
    21601 aaagaacaaa ttcccaatct cttaacaata ggtcgaattc tctttatacc tatttttatc
    21661 tttattttaa cgataggaaa ttcgatagag agtcatatag ttgcagctat tatctttgct
    21721 gttgccagta ttaccgacta tttagatgga tatttagctc gtaaatggaa tgtggtcagt
    21781 aattttggta aatttgcaga tcctatggcg gataagttac tagttatgtc ggcttttatt
    21841 atgttgattg agttaggtat ggctccggct tggattgttg cagtgattat ctgtcgtgag
    21901 ttagctgtga caggtttaag gcttttattg gttgaaactg gtggaacaat tttagcagca
    21961 gcaatgcctg gaaaaattaa aacttttagt cagatgtttg ccattatttt cttgctatta
    22021 cattggactt tgcttggtca agttctactt tatgtagcct tatttttcac tatctactct
    22081 ggctatgact atttcaaggg tagtgcctat gtatttaaag ggacatttgg ttcgaaatga
    22141 aatcaataat tgatgtaaaa aatctttctt ttcgctataa agaaaatcag aactactacg
    22201 atgtgaagga tattacgttt cacgtgaaac gtggagaatg gctttcgatt gtagggcata
    22261 atggtagtgg taaatcaacg acggttcgat taattgatgg cttactggaa gcagaatccg
    22321 gagagattgt aattgatggc caacgtttga ctgaggaaaa tgtttggaat atacgtcgtc
    22381 aaatcggtat ggtttttcaa aatccagaca atcaatttgt tggagcgact gttgaagatg
    22441 atgttgcttt tggtttggaa aatcagggac tttctcgtca agaaatgaaa aagagagtgg
    22501 aagaagctct ggctttagtt ggcatgttgg actttaaaaa gagagagcca gcgcgtctat
    22561 caggtggcca aaagcaacgt gtggccattg caggtgttgt agccctaaga ccagctattt
    22621 taatcttaga tgaagcaacg agtatgttgg atcctgaggg gcgtagagaa cttattggga
    22681 cagtaaaagg aattcgaaaa gactatgata tgacggtcat ttctattacc catgatttgg
    22741 aagaagtcgc catgagtgat cgcgtattgg tcatgaaaaa aggggaaatt gaatcaacta
    22801 gtagtccaag ggagcttttc tctcgaaatg atttagatca aattggatta gacgatcctt
    22861 ttgctaatca attaaaaaaa tttttgagcc agaatggcta taatttacct gaaaattatt
    22921 tgacagaaag tgagctagag gataagctat gggaattgct ctagaaaatg tgaattttac
    22981 atatcaagaa ggtactccct tagcttcagc agctttgtcg gatgtttctt tgacgattga
    23041 agatggctct tatacagctt taattgggca cacaggtagt ggtaaatcaa ctattttaca
    23101 actcttaaat ggtttattgg tgccaagtca agggagtgtg agggtttttg ataccttaat
    23161 cacctcgact tctaaaaata aagatattcg tcaaattaga aaacaggttg gcttggtatt
    23221 tcagtttgct gaaaatcaga tttttgaaga aacggttttg aaggacgttg cttttggacc
    23281 gcaaaatttt ggagtttctg aagaagatgc tgtgaagact gcgcgtgaga aactggctct
    23341 ggttggaatt gatgaatcac tttttgatcg tagtccgttt gagctgtcag ggggacaaat
    23401 gagacgtgtt gccattgcag gcatacttgc catggagcca tctatattag tcttagatga
    23461 gccaacagct ggtctagatc ctctagggag aaaagagttg atgaccctgt tcaaaaaact
    23521 ccaccagtca gggatgacca tcgtcttggt aacgcatttg atggatgatg ttgctgaata
    23581 tgctaatcaa gtctatgtaa tggaaaaggg acgtttagta aaggggggca aaccaagtga
    23641 tgtctttcaa gacgttgttt ttatggaaga agttcagttg ggagtaccta aaattacggc
    23701 cttttgtaaa cgattggctg atagaggcgt gtcatttaaa cgattaccga ttaagataga
    23761 ggagttcaag gagtcgctaa atggatagta tgattttggg gcgttatatc ccaggggatt
    23821 cgattgttca ccgattggat ccacgtagca aattgcttgc tatgatgcta ctgattttga
    23881 ttgtgttttg ggctaataac cccttgacga atctgattct ttttatagcg acagggatat
    23941 ttattgcctt gtcaggagta tctctttcat tttttattca gggcttgaag tctatgtttt
    24001 tcttgattgc cttcacaact atttttcaac tatttttcat ttctaatggg aatgttttat
    24061 ttgagttttc gtttgtgaga atcacggatt atgctttgca acaagctggg attatttttt
    24121 gtcgctttgt attgattatt ttcttttcaa ctttgttaac cttaacaacc atgcctttaa
    24181 gtttggcatc agctgtcgaa gctttattag cacctttaaa gcgtgtgaaa gttccagttc
    24241 atgaaattgg attgatgctg tctatgagtt tgcgttttgt cccaaccttg atggatgata
    24301 cgactcggat tatgaatgca cagaaagctc gtggagtgga ttttggagaa ggaagcatcg
    24361 ttcaaaaagt aaaggcgatg attcccattt tgattcctct ttttgcgaca agtttaaaac
    24421 gtgcagattc cttggctatc gctatggaag cgcgtggcta tcagggtgga aaaggcagaa
    24481 gtcaataccg acaattgaaa tggactctaa aggatacgct gaccattctt gttattctcg
    24541 tacttggttg ttgtttattt ttcttaaaat cttagtagct ttcaggaatt gataaaaagt
    24601 tactgtaaca gtttttgata gaaaggtagg gatatgaacc gttttaaaaa atcaaaatat
    24661 gtcattattg tttttgtcac tgttctgctt gtgtcagctc tcttagcgac gacttattca
    24721 agtacaattg tgacaaaatt aggagatgga atctcattgg ttgatagagt tgtacaaaaa
    24781 ccttttcagt ggtttgattc tgtcaaatca gatttggctc atttgacacg aacatataat
    24841 gaaaatgaaa gtttgaagaa acagctttac caattagaag ttaaatcaaa tgaggtggaa
    24901 agtttaaaga cagaaaatga acaactgcgc caattgcttg atatgaagtc taaattgcaa
    24961 gccacaaaga ctttagcagc agatgttatt atgcgttctc cggtatcttg gaagcaggag
    25021 ttgaccttag atgcaggtag atcaaaaggt gcttctgaga acatgttagc tattgcaaat
    25081 ggtggcttga ttgggagtgt ttcaaaagta gaggagaact ctactatagt caaccttctg
    25141 acaaatacgg aaaatgctga taagatttct gttaaaattc aacatggctc tattacaatt
    25201 tatggaatta ttattggcta tgacaaggaa aatgacgttc ttaaaattag ccaattaaat
    25261 agtaatagcg atattagtgc gggagataag gtgactactg gtggattagg aaactttaac
    25321 gttgctgata ttcctgttgg tgaagtggtt gccacaacgc atagtacaga ctatttgaca
    25381 cgagaagtaa ctgttaaatt gagtgcagat actcataatg tagatgtgat agaattagtg
    25441 gggaattcat aatgagacag ttgaagcgag ttggagtatt tttattgctt cctttctttg
    25501 ttctaattga cgcccatatt agccagcttc tgggctcatt tttcccccat gtacatttgg
    25561 ctagtcattt tctttttcta tttcttttat ttgagacgat agaagtatca gagtatctct
    25621 acctagtcta ttgttttgtt ataggcttgg tttatgatgt ttactttttc catctaatag
    25681 ggattacaac tctcttattt atcttattgg gagccttcct tcataaattg aatagtgtta
    25741 ttttgttgaa tcgttggaca agaatgctag ctatgattgt gctgacattc ctgtttgaaa
    25801 tgggtagtta tcttttggct tttatggtag ggttgacagt agatagcatg tcgattttta
    25861 tagtctatag cttggtaccg acgatgattt taaatttttt atggattact gtttttcaat
    25921 ttatttttga aaaatattat ctataagaac gacatataaa tgtaacaaag gcgtaatatt
    25981 tattaggcct ttttttggta tactagtatt gtctttaaaa gaaggagtat ctacgtaata
    26041 tgaagaaaaa aatcttagcg tcacttttat taagtacagt aatggtttct caagtagctg
    26101 ttttaacaac tgcgcatgca gaaacgactg atgacaaaat tgctgctcaa gataataaaa
    26161 ttagtaactt aacagcacaa caacaagaag cccaaaaaca agttgaccaa attcaggagc
    26221 aagtatcagc tattcaagct gagcagtcta acttgcaagc tgaaaatgat agattacaag
    26281 cagaatctaa gaaactcgag ggtgagatta cagaactttc taaaaacatt gtttctcgta
    26341 accaatcgtt ggaaaaacaa gctcgtagtg ctcaaacaaa tggagccgta actagctata
    26401 tcaataccat tgtaaactca aaatcaatta cagaagctat ttcacgtgtt gctgcaatga
    26461 gtgaaatcgt atctgcaaac aacaaaatgt tagaacaaca aaaggcagat aaaaaagcta
    26521 tttctgaaaa acaagtagca aataatgatg ctatcaatac tgtaattgct aatcaacaaa
    26581 aattggctga tgatgctcaa gcattgacta cgaaacaggc agaactaaaa gctgctgaat
    26641 taagtcttgc tgctgagaaa gcgacagctg aaggggaaaa agcaagtcta ttagagcaaa
    26701 aagcagcagc tgaggcagag gctcgtgcag ctgcggtagc agaagcagct tataaagaaa
    26761 aacgagctag ccaacaacaa tcagtacttg cttcagcaaa cactaactta acagctcaag
    26821 tgcaagcagt atctgaatct gcagcagcac ctgtccgtgc aaaagttcgt ccaacataca
    26881 gtacaaacgc ttcaagttat ccaattggag aatgtacatg gggagtaaaa acattggcac
    26941 cttgggctgg agactactgg ggtaatggag cacagtgggc tacaagtgca gcagcagcag
    27001 gtttccgtac aggttcaaca cctcaagttg gagcaattgc atgttggaat gatggtggat
    27061 atggtcacgt agcggttgtt acagctgttg aatcaacaac acgtatccaa gtatcagaat
    27121 caaattatgc aggtaatcgt acaattggaa atcaccgtgg atggttcaat ccaacaacaa
    27181 cttctgaagg ttttgttaca tatatttatg cagattaatt tacagaggga ctcgaataga
    27241 gccctctttt caggttttac cgtgacaatc cctattaaaa attatatcaa aatagcttga
    27301 aaatattgga aaagtatggt agaatgaaaa ttgtcgtgtg aacgataata ctcattcttg
    27361 atgaattgtg aagcagttgc ccttgggtcg ttttgcgagt tgaagtcaag aagaggaaaa
    27421 aaacaaaaag gagaaatact catggcagta atttcaatga aacaacttct tgaggctggt
    27481 gtacactttg gtcaccaaac tcgtcgctgg aatcctaaga tggctaagta catctttact
    27541 gaacgtaacg gaatccacgt tatcgacttg caacaaactg taaaatacgc tgaccaagca
    27601 tacgacttca tgcgtgatgc agcagctaac gatgcagttg tattgttcgt tggtactaag
    27661 aaacaagcag ctgatgcagt tgctgaagaa gcagtacgtt caggtcaata cttcatcaac
    27721 caccgttggt tgggtggaac tcttacaaac tggggaacaa tccaaaaacg tatcgctcgt
    27781 ttgaaagaaa ttaaacgtat ggaagaagat ggaactttcg aagttcttcc taagaaagaa
    27841 gttgcacttc ttaacaaaca acgtgcgcgt cttgaaaaat tcttgggcgg tatcgaagat
    27901 atgcctcgta tcccagatgt gatgtacgta gttgacccac ataaagagca aatcgctgtt
    27961 aaagaagcta aaaaattggg aatcccagtt gtagcgatgg ttgacaccaa tactgatcca
    28021 gatgatatcg atgtaatcat cccagctaac gatgacgcta tccgtgctgt taaattgatc
    28081 acagctaaat tggctgacgc tattatcgaa ggacgtcaag gtgaggatgc agtagcagtt
    28141 gaagcagaat ttgcagcttc agaaactcaa gcagattcaa ttgaagaaat cgttgaagtt
    28201 gtagaaggtg acaacgctta atttatacaa atagtaatta cctaggaggg cggggcttag
    28261 cccggctctc ctattttcaa aaaatatagg agaattaaaa tggcagaaat tacagctaaa
    28321 cttgtaaaag agttgcgtga aaaatctggt gccggtgtta tggacgctaa aaaagcgctt
    28381 gtagaaacag acggtgacat cgaaaaagcg attgaattgc ttcgtgaaaa aggtatggct
    28441 aaggcagcta agaaagctga ccgtgttgct gcagaaggtt tgactggtgt ttatgttaac
    28501 ggtaatgttg cagcagttat tgaagtaaac gctgaaactg acttcgttgc aaaaaacgct
    28561 caattcgttg aattggtaaa tactacagct aaagtcattg ctgaaggaaa acctgctaac
    28621 aatgaagaag ctcttgcttt gataatgcct tcaggtgaaa ctcttgaagc tgcatacgta
    28681 tctgcaacag caactatcgg agagaaaatc tcattccgtc gctttgcatt gattgaaaaa
    28741 acagacgcac aacactttgg agcataccaa cataacggtg gacgtatcgg tgttatttca
    28801 gttgttgaag gtggagacga agcacttgct aaacaattgt caatgcacat cgcagcgatg
    28861 aaaccaacag ttctttctta caaagaattg gatgagcaat tcgttaaaga tgagttggca
    28921 caattgaatc acgttatcga ccaagacaac gaaagccgtg caatggttaa taaaccagct
    28981 cttccacact tgaagtatgg atcaaaagct caattaactg atgatgttat tgctcaagct
    29041 gaagctgaca tcaaagctga attggctgca gaaggcaaac cagaaaaaat ctgggacaaa
    29101 attattccag gtaaaatgga tcgcttcatg cttgataaca ctaaagttga ccaagcttac
    29161 acacttcttg cacaagttta catcatggat gacagcaaga cagttgaagc ataccttgaa
    29221 tcagttaacg cttcggtagt tgagtttgct cgctttgaag ttggtgaagg tatcgagaaa
    29281 gctgcaaacg acttcgaagc tgaagttgca gctacaatgg cagcagcctt gaataactaa
    29341 taatagaaag gaagcaaatt gcttcctttt ttgtataggg cttagatttt tcctgaaatt
    29401 gttttttccc cagtctcact cgattagaat aattaaaaca aaaagcccta taataagggc
    29461 taagggcatt taggagacta tacttcaaat tcatagtaag cgcgttcata acaaggtatc
    29521 tatcctaatt ccccaacttc cagtatacta gataaaaaat aattatcgga ggaaaggtat
    29581 tgtactatga taattcaact aagtgattta ggtcaagttc accttgtttg tagcaagata
    29641 gatatgaggc agggaataga ctcattagcc tatgtagtta aaacccactt tgaattggat
    29701 cctttctccg gtcaagtctt tctcttttgt ggtggacgta aagaccgctt taaagccctt
    29761 tactgggatg gtcaaggatt ttggctacta tataaacgct ttgagaacgt cagactgact
    29821 tggcccagta cagaaaagga tgtcaaagct ctcacacctg aacaagtaga ctggcttatg
    29881 aagggctttt ctatcactcc aaaaataaat ccatcagaaa gtcgtgattt ctattgaaat
    29941 gaggactttc tttttatact catctgcttt caaaaagcat tctagtccat ctccgattaa
    30001 cgatggactt tatcacctcc ttctccagtc cttgtataac atcttggagt tgattcatga
    30061 catcttccaa agtttaaaag gctttattct taaatccacg tttacgaatc tctttccaca
    30121 cttgttcaat ggggttcatc tctggtgtgt atggaggaat aaatgcaaag ccaatattag
    30181 tcggaatctt taaggtactt gatttatgcc atatagcatt gtccataacg agtaaaagat
    30241 aatcatctgg ataagcttgt gaaatctcct attcctaaag cccctttagc gcataacttt
    30301 ggctcagctt ctattatcgc tcacaccatc catcagaagt ttaatctgaa ggtacccaat
    30361 tatcgccaag aagaagattg ggctaggatg ggtttaccaa tcacacgtaa ggaaatctct
    30421 aatcggcata tcaagacgag tcaatactat ttggagcccc tttataacct cttgcgagag
    30481 agactattga ctcagccctt acttcatgcg gatgaaactt cttatagggt gctagagagt
    30541 gatagtcagc tgacttacta ttggactttt ttgtctggga aagctgagaa acaagggatt
    30601 acgctttacc atcatgtcct aatcgatttg ttcatatctt atttcaatcc attataaata
    30661 gtgagaaata tctatcctat cttctagaat gtcttccaaa cgtggaaact ctcgtaaaca
    30721 aagaggtttt agaggcctat ttaccatgga ctaaagttgt acaagaaaag tgcaaataag
    30781 aaatctccag attaggaact atccgtgagt tctctagtct ggagattttt caatagactt
    30841 cgttattgga cggttacaat tcataaagtg ctgtagagag ataacgttca ccgttatctg
    30901 gtgctagggc aaggactttt ttacctgtac ctaatttttt ggcaacctcg atggctccgt
    30961 agatagctgc agctgaggaa atccctacaa ggaagccttc ttttccacca atttcacgtc
    31021 cgagtgcaag agcgtcatct gatgttacac gaacgatacc atcataggct ttagtatcaa
    31081 gtgtatcagg aataaatcca gctgagatac cttgaatttt gtgaggacca ggtttttcac
    31141 cagatagaat agcagattca tctgcttcta ctgcaaaaac ttgaatgtta gaattttctg
    31201 atttgagtgc atgagaaaca ccagaaatcg ttccaccggt acctactcca gcaacaaagg
    31261 catctaatcc atctttaccg aaagcagcta gtatctcagc tcctgttgtt ctttcgtgta
    31321 cttctggatt agctggattg tcaaattgaa gaggaaggaa accatcacgt tcagcagcga
    31381 tttcttgagc cttagcaata gcacctttca ttccctcgct accaggagtt aggacgagtt
    31441 cagcaccata agcttggata attttacgtc gttctacact catagtttca ggcataacga
    31501 tgacgacttt atacccttta gcagcaccta cccatgaaag tccaatacca gtgtttccac
    31561 ttgttgcttc aacaatagta gaaccaggtt tcagaatacc atcttgttca gctttttcaa
    31621 tcatgctaag ggcaatacgg tcttttacag atgaaccagg attaaatgct tcaagcttta
    31681 tatagacgtc tgcagcacct tctggcacga tgttgttaag tttaacaatc ggtgtttgac
    31741 cgattaattc agtaatgttg ttataaatag acatatgaat acctctttgt ataagatatc
    31801 tttagtataa cgctatccat accatttgta aaatatagaa atcctatcga agcgatagga
    31861 tttctatata tcatagatca aagtcattaa ttttcaagtg attgcgttca gctaattcta
    31921 gtaaataggt gtgaaggaaa acgatatcag gtttcgtttg aattgcttca ttttctagta
    31981 tattttggta cgcatctttt tattttgctc gctaaagtag ttgtgagaat gtcaacgaaa
    32041 cgctgtatta ttcattgttt acactaagtt tacaggaact tcaacctctc gtaaaccttg
    32101 gtcagttaaa gtgacttttc cattaaaaaa ctccacaagt gcagctttaa tagtttcttt
    32161 ttcttcttta tcaacataaa tcatcgtatc gacttgatct gtaaagtttg tatccagctc
    32221 catgagatca tgttctttaa ggaagttact gtactcttgg tactgagcat aagacatttg
    32281 aatagcaatg ccagcctgtt cttttatttc aataatacca atttctttga cagctaaggc
    32341 gacactgccg gcgtaagcac gaattagtcc tccagcgcct agtttaatac caccaaagta
    32401 gcgtgtcacg accacacaga cattggtgag attgtgattt tctagtaccc caagcatggg
    32461 aacaccagca gtaccactag gctcaccatc atcacttgta cgtttaattt cactacgttc
    32521 tccaataata aaggcagagc agttatgtgt cgctttgtag tgttcttttt tgatggcagt
    32581 aatgaagtca cgagcctctt cttcgctata aacacgcttg gcatggcaga taaagcgaga
    32641 ttttttgatt tcttcttgga cttggtcgtc ctctttaatt gttctaaatt ccatgattta
    32701 attgtactaa aaaatcatct aaagtgctag tttttacgaa taaagaagta tgaaagtaaa
    32761 tttagattat ctcggtcgtt tatttactga gaatgaatta acagaagaag aacgtcagtt
    32821 ggcggagaaa cttccagcaa tgagaaagga gaaggggaaa cttttctgtc aacgttgtaa
    32881 tagtactatt ctagaagaat ggtatttgcc catcggtgct tactattgtc gagagtgctt
    32941 gctgatgaag cgagtcagaa gtgatcaaac tttatactat tttccgcagg aggattttcc
    33001 gaagcaagat gttctcaaat ggcgcggcca attaactcct tttcaagaga aggtgtcaga
    33061 gggactgctt caagcagtag acaagcaaaa gccaacctta gttcatgcgg taacaggagc
    33121 tggaaagaca gaaatgattt atcaagtagt ggctaaagtg atcaatgcgg gtggtgcagt
    33181 gtgtttggct agtcctcgca tagatgtttg tttggagctg tacaagcgcc tgcaacagga
    33241 tttttcttgc gggatagctt tgctacatgg agaatcggaa ccttattttc gaacaccact
    33301 agttgttgca acaacccatc agttattgaa gttttatcaa gcttttgatt tgctgatagt
    33361 ggatgaagta gatgcttttc cttatgttga taatccaatg ctttaccacg ctgtcaagaa
    33421 tagtgtaaag gagaatggat tgagaatctt tttaacagcg acttcgacca atgagttaga
    33481 taaaaaggtc cgtttaggag aactaaaaag actgaattta ccgagacggt ttcatggaaa
    33541 tccgttgatt attccaaaac caatttggtt atcggatttt aatcgctact tagacaagaa
    33601 tcgtttgtca ccaaagttaa agtcctatat tgagaagcag agaaagacag cttatccgtt
    33661 actcattttt gcttcagaaa ttaagaaagg ggagcagtta gaagaaatct tacaggagca
    33721 atttccaaat gagaaaattg gctttgtatc ttctgtaaca gaggatcgat tagagcaagt
    33781 acaagctttt cgagatggag aactgacaat acttatcagt acgacaatct tggagcgtgg
    33841 agttaccttc ccttgtgtgg atgttttcgt agtagaggcc aatcatcgtt tgtttaccaa
    33901 gtctagtttg attcagattg gtggacgagt tggacgaagc atggatagac cgacaggaga
    33961 tttgcttttc ttccatgatg ggttaaatgc ttcaatcaag aaggcgatta aggaaattca
    34021 gatgatgaat aaggaggctg gtctatgaag tgcttgttat gtgggcagac tatgaagact
    34081 gttttaactt ttagtagtct cttacttctg aggaatgatg actcttgtct ttgttcagac
    34141 tgtgattcta cttttgaaag aattggggaa gagaactgtc caaattgtat gaaaacagag
    34201 ttgtcaacaa agtgtcaaga ttgtcaattt tggtgtaaag aaggagttgg agtcagtcat
    34261 agagcgattt ttacttacaa tcaagctatg aaggattttt tcagtcggta taagtttgat
    34321 ggagacttcc tgttaagaaa agttttcgct tcatttttaa gtgaggagtt gaaaaagtac
    34381 aaagagtatc aatttgttgt aattccccta agtcctgata gatatgctaa tagaggattt
    34441 aatcaggttg agggcttggt agaggcagca ggttttgagt atctggattt attagagaaa
    34501 agagaagaga gagccagttc ttctaaaaat cgttcagagc gcttggggac agaacttcct
    34561 ttctttatta aaagtggagt cactattcct aaaaaaatcc tacttataga tgatatctat
    34621 actacaggag caactataaa tcgtgttaag aaactgttgg aagaagctgg tgctaaggat
    34681 gtaaaaacat tttcccttgt aagatgagga aaaaatttgc aaaaatagaa tgaaagcgtt
    34741 ataataaaat cataaaaaca aaaatgttta gaaagaaggt actcatatga ttaaatatag
    34801 tatccgtggt gaaaacctag aagtaacaga agcaattcgt gattatgtag tttctaaact
    34861 cgaaaagatc gaaaagtact tccaaccaga acaagagttg gatgcccgaa ttaacttaaa
    34921 agtttatcgt gaaaaaacgg ctaaagtgga agtaacgatt ccgcttggat ctattactct
    34981 ccgcgcagaa gatgtatctc aagatatgta tggttcaatt gaccttgtaa ctgataaaat
    35041 tgaacgtcag attcgtaaaa ataaaacaaa aatcgagcgt aaaaataaaa ataaggtagc
    35101 aactggtcaa ttatttacag atgctttggt ggaagattca aatgttgtcc agtctaaagt
    35161 tgttcgttca aaacaaattg atttaaaacc aatggatttg gaagaagcaa ttctacaaat
    35221 ggatttattg gggcatgatt tctttatcta tgtggatgtt gaagatcaga caaccaatgt
    35281 gatttatcgt cgtgaggatg gcgaaattgg tttgttagag gttaaagaat cttaatttca
    35341 atatgtgtaa aggtaggttt actgaattgt aaactacctt tttcttataa ttgatagaat
    35401 tactgattac acttttaaaa agtcgctttt tgaggggtat tatggtataa tgggtgccaa
    35461 gaggttttga atgaaaaaat tttatgtaag tccaattttt cctattctag taggattgat
    35521 tgcgtttgga gtcttatcca ctttcattat ttttgttaat aataatctgt tgacggtttt
    35581 aattttgttt ctttttgtag gaggctatgt ttttttattt aagaaactga gagtgcatta
    35641 tacaaggagt gatgtagaac agatacagta tgtaaaccac caagcggaag aaagtttgac
    35701 atctctattg gaacagatgc ctgtaggtgt tatgaaattg aatttatctt ctggagaggt
    35761 tgagtggttt aatccctatg ctgaattgat tttgaccaag gaagatggtg attttgattt
    35821 agaagctgtt caaacgatta ttaaggcttc agtaggaaat ccgtctactt atgccaagct
    35881 tggtgagaag cgttatgctg ttcatatgga tgcttcttcc ggtgttttgt attttgtaga
    35941 tgtatccagg gaacaagcca taacagatga attggtaaca agtcgaccag tgattgggat
    36001 tgtctctgtg gataattatg atgatttgga ggatgcaact tctgagtcag atattagtca
    36061 aatcaatagt tttgtagcta attttatatc agagttttca gaaaaataca tgatgttttc
    36121 tcgtcgggta agtatggatc gattttatct atttactgac tacacggtgc ttgagggctt
    36181 gatgaatgat aaattttctg ttattgatgc tttcagagaa gagtcgaaac agagacagtt
    36241 gcccttgacc ttaagtatgg gattttctta tggcgatgga aatcatgatg agatagggaa
    36301 agttgctttg ctcaatttga acttggctga agtacgtggt ggcgaccagg tggttgttaa
    36361 ggaaaacgac gaaacgaaaa atccagttta ttttggtggt gggtctgctg cttcaatcaa
    36421 gcgtacacgg actcgtacgc gcgctatgat gacagctatt tcagataaga ttcggagtgt
    36481 agatcaggtt tttgtagtcg gtcacaaaaa tttagacatg gatgctttgg gctctgctgt
    36541 aggtatgcag ttgttcgcca gcaatgtgat tgaaaatagc tatgctcttt atgatgaaga
    36601 acaaatgtct ccagatattg aacgagctgt ttcattcata gaaaaagaag gagttacgaa
    36661 gttgttgtct gttaaggatg caatggggat ggtgaccaat cgttctttgt tgattcttgt
    36721 agaccattca aagacagcct taacattatc aaaagaattt tatgatttat ttacccaaac
    36781 cattgttatt gaccaccata gaagggatca ggattttcca gataatgcgg tcattactta
    36841 tatcgaaagt ggtgcaagta gtgccagtga gttggtaacg gaattgattc agttccagaa
    36901 ttctaagaaa aatcgtttga gtcgtatgca agcaagtgtt ttgatggctg gtatgatgtt
    36961 ggatactaaa aatttcacct cgcgagtaac tagtcggaca tttgatgttg ctagctatct
    37021 cagaacgcgc ggaagtgata gtattgctat ccaggaaatc gctgcgacag attttgaaga
    37081 atatcgtgag gtcaatgaac ttattttaca ggggcgtaaa ttaggttcag atgtactaat
    37141 agcagaggct aaggacacga aatgctatga tacagttgtt attagtaagg cagcagatgc
    37201 catgttagcc atgtcaggta ttgaagcgag ttttgttctt gcgaagaata cacaaggatt
    37261 tatctctatc tcagctcgaa gtcgtagtaa actgaatgta caacggatta tggaagagtt
    37321 aggcggtgga ggccacttta atttggcagc agctcaaatt aaagatgtaa ccttgtcaga
    37381 agcaggtgaa aaactgacag aaattgtatt aaatgaaata aaggaaaagg agaaagaaga
    37441 atgaaagtaa tctttttagc agatgttaaa ggaaaaggta aaaaaggcga aattaaggaa
    37501 gtaccaacag ggtatgcgca aaactttctt atcaaaaaga atctagccaa agaagcgact
    37561 gctcaagctg taggtgaact tcgtggtaaa caaaaatcgg aagaaaaagc tcacgctgag
    37621 atgattgcag aaggaaaagc aattaaagca caacttgaag cagaagaaac tgttgtagaa
    37681 tttgttgaaa aagttggtcc agatggtcgt acctttggtt ctattaccaa taagaagatt
    37741 gcagaagaat tgcaaaagca atttggaatt aagattgata aacgtcatat tcaagtacaa
    37801 gctccgattc gagcggttgg tttgattgat gtgccagtga aaatctatca agatatcaca
    37861 agtgtaatca atcttcgtgt gaaagaagga taagtttaca ccttcttgac aagattgtaa
    37921 aaggaaggga agtctgatgg cagaagtaga agagttacga gtacaacctc aagatatctt
    37981 agctgagcaa tccgttttag gggctatctt tattgatgag agtaaacttg tttttgtgcg
    38041 agaatacatt gagtctcggg acttttttaa gtatgcccat cgtttgattt tccaagccat
    38101 ggtcgattta tccgatcgtg gtgatgccat agatgcaaca acggttcgta ctatccttga
    38161 taatcaaggt gatttacaga atattggtgg cttgtcttac ttggttgaga ttgtcaattc
    38221 tgtgccaact tctgctaatg cggagtatta tgctaagatt gttgcagaaa aagcaatgct
    38281 acgtcgttta attgccaagt tgacagagtc tgtcaaccaa gcttacgaag cgtcacaacc
    38341 agctgatgaa attattgctc aggcagaaaa agggctgatt gatgtcagtg aaaatgcaaa
    38401 tcgaagcggg tttaagaaca ttcgagatgt gttgaatctc aactttggaa atctggaagc
    38461 tcgctcgcaa caaacgaccg atattacagg tattgcgaca ggttatcgtg atttggatca
    38521 tatgacaaca ggacttcatg aggaggagtt gattatctta gcagctcgtc cagcagttgg
    38581 taagacagca tttgccttga atatcgctca gaacattggg actaagttgg acaaaacggt
    38641 tgctattttt tcactcgaaa tgggtgcgga aagcttggta gatcgtatgt tagctgcaga
    38701 aggcttagtg gagtcacatt ctatccgtac agggcaattg acagatgagg agtggcaaaa
    38761 atatactatt gctcagggta atctagctaa cgccagtatc tatatcgatg atacgccagg
    38821 tattcggatt acagagattc gttctcgttc tcgtaaattg gctcaagaaa ctggaaatct
    38881 tggtttgatt ttgatagact atttgcaact tatcacggga actggtcgag aaaatcgtca
    38941 acaagaagtt tctgaaattt ctcgtcagtt gaaaatacta gccaaggaat tgaaggttcc
    39001 agtaatcgct ctgagtcagc tttctcgtgg tgtagaacaa cgtcaggaca agagaccggt
    39061 cttgtctgat attcgtgaat ctgggtctat tgagcaggac gctgatatcg tagcttttct
    39121 ctatcgcgat gactactatg aacgtggtgg tgaagaagag gagggtatcc caaataataa
    39181 ggtggaagtt attatcgaga aaaaccgtag tggagctcgt ggaacagtgg aattgatttt
    39241 ccaaaaagaa tacaataaat tttcaagtat ctcaaagagg gaggcataag atgtcagatg
    39301 catttacaga tgtagccaag atgaaaaaaa tcaaagaaga aatcaaggca catgagggac
    39361 aagtcgtaga aatgactttg gagaatggtc gtaagcgcca aaaaaataga ttgggtaagc
    39421 taattgaagt ttatccatcc ctatttattg tggagtttgg ggatgtggaa ggagataaac
    39481 aagttaatgt ttacgttgaa tcctttactt actcagatat tcttacagaa aagaatttga
    39541 ttcattatct tgactaaagt gagaaatttt ctcacttttt cttttttctc cgaataattt
    39601 aggtgaaggc aatcatcgct ttatattatt tttcaaggag gaagaatgaa aattttaccg
    39661 tttatagcaa gaggaacaag ttattacttg aagatgtcag ttaaaaagct tgttcctttt
    39721 ttagtagtag gattgatgct agcagctggt gatagtgtct atgcctattc cagaggaaat
    39781 ggatcgattg cgcgtgggga tgattatcct gcttattata aaaatgggag ccaggagatt
    39841 gatcagtggc gcatgtattc tcgtcagtgt acttcttttg tagcctttcg tttgagtaat
    39901 gtcaatggtt ttgaaatgcc ggcagcttat ggaaatgcga atgaatgggg acatcgtgct
    39961 cgtcgggaag gttatcgtgt agataataca ccgacgattg gttccattac ttggtctact
    40021 gcaggaactt atggtcatgt tgcctgggtg tcaaatgtaa tgggagatca gattgagatt
    40081 gaggaatata actatggtta tacagaatcc tataataaac gagttataaa agcaaacacg
    40141 atgacaggat ttattcattt taaagatttg gatggcggca gcgttgggaa tagtcaatcc
    40201 tcagcttcaa caggcggaac tcattatttt aagaccaagt ctgctattaa acctgaaccc
    40261 ctagctagcg gaactgtgat tgattactat tatcctgggg agaaggttca ttatgatcag
    40321 atatttgaaa aagacggcta taagtggttg agttatactg cctataatgg aagctatcgt
    40381 tatgttcaat tggaggctgt gaataaaaat cctctaggta attctgttct ttcttcaaca
    40441 ggcggaactc attattttaa gaccaagtct gctatcaaaa ctgaacccct agttagtgca
    40501 actgtgattg attactatta tcctggagag aacgttcatt atgatcaaat tctcgaaaaa
    40561 gacggctaca agtggttgag ttatacggct tataacggaa gtcgtcgcta tatacagcta
    40621 gagggagtga cttcttcaca aaattatcag aatcaatcag gaaacatctc tagctatgga
    40681 tcccatagta gttcaactgt cggttggaag aaaataaatg gtagttggta tcatttcaaa
    40741 tcaaatggtt ctaaatcaac aggatggctg aaagacggtt ctagctggta ttatttgaaa
    40801 ttatctggtg aaatgcagac aggatggtta aaggaaaatg gtttgtggta ttatctgggt
    40861 agttcagggg caatgaaaac aggctggtac caggtctctg gtaagtggta ttattcttac
    40921 tcttcaggcg ccttagctgt taatacgacg gtggatggct acagagtaaa cagtgatgga
    40981 gaacgagtat agaaaattgg agtaggagaa atttcctgct cttttctgta agcagtttcc
    41041 ttgacatttt tcttattttg cgctatcata tatacatata atatatggga gtctgtgtac
    41101 agtctgagag gaagtgttaa acttcgaccg cacctgatct gggtaatgcc agcggaggga
    41161 acgatactta gtctaatttt gcaccttttc catgtatggt aaaggttttt ctttttttaa
    41221 aaaggaaaac gagaagagga ggttcttatg aaagcaagca ttgccttgca agttttaccc
    41281 ctagtacagg ggattgatcg gatagctgtt attgatcagg tcattgctta tctgcaaact
    41341 caagaagtga cgatggtagt gacaccattt gaaacggtct tggaagggga gtttgatgag
    41401 cttatgcgca ttctaaaaga agcgctggaa gtggcagggc aggaggcaga caatgtcttt
    41461 gccaatgtca aaataaatgt aggagagatt ttaagtattg atgagaaact tgagaagtat
    41521 actgagacga cacattagtc tattgggctt tctcggagta ttgtcaatct ggcagttagc
    41581 aggttttctt aaacttctcc ccaagtttat cctgccgaca cctcttgaaa ttctccagcc
    41641 ctttgttcgt gacagagaat ttctctggca ccatagctgg gcgaccttga gagtggcttt
    41701 actggggctg attttgggag ttttgattgc ctgtcttatg gctgtgctca tggatagttt
    41761 gacttggctc aatgacctga tttaccctat gatggtggtc attcagacca ttccgaccat
    41821 tgccatagct cctatcctgg tcttgtggct aggttatggg attttgccca agattgtctt
    41881 gattatctta acgacaacct ttcccatcat cgttagtatt ttggacggtt ttaggcattg
    41941 cgacaaggat atgctgacct tgtttagtct gatgcgggcc aagccttggc aaatcctgtg
    42001 gcattttaaa atcccagtta gcctgcctta cttttatgca ggtctgaggg tcagtgtctc
    42061 ctacgccttt atcacaactg tggtatctga gtggttggga ggttttgaag gtcttggtgt
    42121 ttatatgatt cagtctaaaa aactgtttca gtatgatacc atgtttgcca ttattattct
    42181 ggtgtcgatt atcagtcttt tgggtatgaa gctggtcgat atcagtgaaa aatatgtgat
    42241 taaatggaaa cgttcgtaga attagaatgt ttctgaaaaa gaaaagagga aatcaaaatg
    42301 aagaaaacat ggaaagtgtt tttaacgctt gtaacagctc ttgtagctgt tgtgcttgtg
    42361 gcctgtggtc aaggaactgc ttctaaagac aacaaagagg cagaacttaa gaaggttgac
    42421 tttatcctag actggacacc aaataccaac cacacagggc tttatgttgc caaggaaaaa
    42481 ggttatttca aagaagctgg agtggatgtt gatttgaaat tgccaccaga agaaagttct
    42541 tctgacttgg ttatcaacgg aaaggcacca tttgcagtgt atttccaaga ctacatggct
    42601 aagaaattgg aaaaaggagc aggaatcact gccgttgcag ctattgttga acacaataca
    42661 tcaggaatca tctctcgtaa atctgataat gtaagcagtc caaaagactt ggttggtaag
    42721 aaatatggga catggaatga cccaactgaa cttgctatgt tgaaaacctt ggtagaatct
    42781 caaggtggag actttgagaa ggttgaaaaa gtaccaaata acgactcaaa ctcaatcaca
    42841 ccgattgcca atggcgtctt tgatactgct tggatttact acggttggga tggtatcctt
    42901 gctaaatctc aaggtgtaga tgctaacttc atgtacttga aagactatgt caaggagttt
    42961 gactactatt caccagttat catcgcaaac aacgactatc tgaaagataa caaagaagaa
    43021 gctcgcaaag tcatccaagc catcaaaaaa ggctaccaat atgccatgga acatccagaa
    43081 gaagctgcag atattctcat caagaatgca cctgaactca aggaaaaacg tgactttgtc
    43141 atcgaatctc aaaaatactt gtcaaaagaa tacgcaagcg acaaggaaaa atggggtcaa
    43201 tttgacgcag ctcgctggaa tgctttctac aaatgggata aagaaaatgg tatccttaaa
    43261 gaagacttga cagacaaagg cttcaccaac gaatttgtga aataatgaca gaaattagac
    43321 tagagcacgt cagttatgcc tatggtcagg agaggatttt agaggatatc aacctacagg
    43381 tgacttcagg cgaagtggtt tccatcctag gcccaagtgg tgttggaaag accaccctct
    43441 ttaatctaat cgctgggatt ttagaagttc agtcagggag aattgtcctt gatggtgaag
    43501 aaaatcccaa ggggcgcgtg agttatatgt tgcaaaagga tctgctcttg gagcacaaga
    43561 cggtgcttgg aaatatcatt ctgcccctct tgattcaaaa ggtggataag gcagaagcta
    43621 tttcccgagc ggataaaatt cttgcgacct tccagctgac agctgtaaga gacaagtatc
    43681 ctcatgaact tagcggtggg atgcgccagc gtgtagcctt actccggacc tacctttttg
    43741 ggcacaagct ctttctctta gatgaggcct ttagcgcctt ggatgagatg acaaagatgg
    43801 aactccacgc ttggtatctt gagattcaca agcagttgca gctaacaacc ctgatcatca
    43861 cgcatagtat tgaggaggcc ctcaatctca gcgaccgcat ctatatcttg aaaaatcgcc
    43921 ctgggcagat tgtttcagaa attaaactag attggtctga agatgaggac aaggaagtcc
    43981 aaaagattgc ctacaaacgt caaattttgg cggaattagg cttagataag tagaaaaata
    44041 gggagttggt gaagattatc ctttaccagc gccctttttc ttttaaaaat gagaaaattt
    44101 cggtataata gtcaaacaag gtcaaggttt aaagagagag gtgggtttgt tatgagattt
    44161 aaaaatacat cggatcatat tgaggcctac atcaaggcga ttttagatca atctggtatc
    44221 gtggagttgc aacggagtca gttggcagat acctttcagg ttgttcctag tcagattaac
    44281 tacgtgatca agacacgctt tacggaaagt agaggctact tggttgaaag taagcgtggt
    44341 ggcggaggct acattcgtat aggacggatt gagttttcta gtcatcatga aatgctccgg
    44401 gagctgcttt actcgattgg tgagcgagtc agtcaagaaa tttatgagga tattctccag
    44461 cttttggttg agcaggaatt gatgaccaag caggagatga atttgctaga atcagtagct
    44521 ttggatcgcg ttttaggaga agaagctcca gttgttcgag caaacatgct acgtcagatc
    44581 atacaagagg tagatagaaa agggaagtaa gatgaactat tcaaaagcat tgaatgaatg
    44641 tatcgaaagt gcctacatgg ttgctggaca ttttggagct cgttatctag agtcttggca
    44701 cttgttgatt gccatgtcta atcacagtta tagtgtagca ggggcaactt taaatgatta
    44761 tccgtatgag atggaccgtt tagaagaggt ggctttggaa ctgactgaaa cggactatag
    44821 ccaggatgaa acctttacgg aattgccgtt ctcccgtcgt ttgcaggttc tttttgacga
    44881 agcagagtat gtagcgtcag tggtccatgc taaggtgcta gggacagagc acgtcttcta
    44941 tgcgattttg catgatggca atgccttggc aactcgtatc ttggagaggg ctggtttttc
    45001 ttatgaagac aagaaagatc aggtcaagat tgctgccctt cgtcgaaatt tagaagaacg
    45061 ggcaggctgg actcgtgaag acctcaaggc tttacgccaa cgccatcgta cagtagctga
    45121 caagcaaaat tctatggcca atatgatggg catgccgcag actcctagtg gtggtcttga
    45181 ggactatacg catgatttga cagagcaagc gcgttctggc aagttagaac cagtcatcgg
    45241 tcgggacaag gaaatctcac gtatgattca aattttgagc cgtaagacta agaacaaccc
    45301 tgtcttggtt ggggatgctg gtgtcgggaa aacagctctg gcccttggtc ttgcccagcg
    45361 tattgctagt ggtgacgtgc ctgcggaaat ggctaagatg cgcgtgttag aacttgattt
    45421 gatgaatgtc gttgcaggga cacgcttccg tggtgacttt gaagaacgca tgaataatat
    45481 catcaaggat attgaagaag atggccaagt catcctcttt atcgatgaac tccacaccat
    45541 catgggttct ggtagcggga ttgattcgac tctggatgcg gccaatatct tgaaaccagc
    45601 cttggcgcgt ggaactttga gaacggttgg tgccaccact caggaagaat atcaaaaaca
    45661 tatcgaaaaa gatgcggctc tttctcgtcg tttcgctaaa gtgacgattg aagaaccaag
    45721 tgtggcagat agtatgacta ttttacaagg tttgaaggcg acttatgaga aacatcaccg
    45781 tgtacaaatc acagatgaag cggttgaaac agcggttaag atggctcatc gttatttaac
    45841 cagtcgtcac ttgccagact ctgctatcga cctcttggac gaagcagcag caacagtgca
    45901 aaataaggca aagcatgtaa aagcagacga ttcagatttg agtccagctg acaaggccct
    45961 gatggatggc aagtggaaac aggcagccca gctaatcgca aaagaagagg aagtacctgt
    46021 ctacaaagac ttggtgacag agtctgatat tttgaccacc ttgagtcgct tgtcaggaat
    46081 cccagttcaa aaactgactc aaacggatgc taagaagtat ttaaatcttg aagcagaact
    46141 ccataaacgt gtcatcggtc aagatcaagc tgtttcaagt attagtcgtg ccattcgccg
    46201 taatcagtca ggaattcgca gtcacaagcg tccgatcggt tcctttatgt tcctaggacc
    46261 cacaggtgtc ggtaagaccg agttggccaa ggctttggca gaagttctct ttgatgacga
    46321 atcagccctt atccgctttg atatgagtga gtatatggag aaattcgcag ccagccgtct
    46381 aaatggagct cctccaggct atgtgggtta cgaagaaggt ggggagctga cagagaaggt
    46441 tcgcaataaa ccctattccg ttctcctctt tgatgaggta gagaaggccc acccagatat
    46501 ctttaatgtt ctcttgcagg ttctggatga cggtgtcttg acagatagca agggacgcaa
    46561 ggtcgatttt tcaaatacca ttatcattat gacatcgaat ctaggtgcga ctgcccttcg
    46621 tgatgataag actgttggtt ttggggctaa ggatattcgt tttgaccagg aaaatatgga
    46681 aaaacgcatg tttgaagaac tgaaaaaagc ttatagaccg gaattcatca accgcattga
    46741 tgagaaggtg gtcttccata gcctatctag tgaccatatg caggaagtgg tgaagattat
    46801 ggtcaagcct ttagtggcaa gtttggctga aaaaggcatt gacttgaaat tacaagcttc
    46861 agctctgaaa ttgttagcaa atcaaggata tgacccagag atgggagctc gcccacttcg
    46921 cagaaccctg caaacagaag tggaggacaa gttggcagaa cttcttctta agggagattt
    46981 agtggcaggc aacacactta agattggtgt caaagcaggc cagttaaaat ttgatattgc
    47041 ataaaagaat aaaagtatca gcatctgacc ataagtcaca gtggagtgaa actcaatgaa
    47101 aatcaaagag caaactaggc agctagctgc aggttgctca aaacactgtt ttgaggttgc
    47161 agatagagct gacgtggttt gaagagattt tcgaagagta tgaaactaaa acctatagct
    47221 tctaaacgat ccgtggtttt caccattcaa tacaaaattc atatgtttat taccctccgt
    47281 cgtatttgtc ttagagcgtg tgtagtagaa aaagagcagt cttatctgaa atttttattc
    47341 tttcaaaaga gacctgtttc ttttttgcat gtcaaatccg ttctagctgg tatttgaaaa
    47401 atcaaactaa tattcaatga aaatcaaaga acaaactagg aagctagccg caggttgctc
    47461 aaaacactgt tttgaggttg tagatagagc tgacgtggtt tgaagagatt ttcgaagagt
    47521 ataagctgca agatgaatga ttttcttgta ttgacgttgt tgttgacaaa aagtagcgga
    47581 taaatgaaat ccattccatt atcatagatg ataggctggt aggaaatttt caaatagcat
    47641 acaggaaata gatgtatgga gttctggtag tagaaaggga gagagatgaa cattttagtt
    47701 gcagatgacg aggaaatgat tagagaagga attgcagcat ttctgacaga agagggttat
    47761 catgtcatta tggctaagga tggacaagag gtcttggaaa aatttcaaga tctccctatc
    47821 catctcatgg tactggattt aatgatgcct aggaagagtg gttttgaagt gttaaaagaa
    47881 atcaatcaaa agcacgatat tcctgtcatc gtcttgagtg ctctgggaga tgaaactact
    47941 cagtcacagg tatttgatct ctatgctgat gatcatgtga caaaaccttt ttctttggta
    48001 ctgcttgtca agcgtattaa ggcgcttatc agacgttact acgtcataga ggatctttgg
    48061 cgatatcagg atgtaacagt ggattttacc tcttacaaag cacattataa aaatgaagaa
    48121 attgatctca aaccaaagga attactggta ctaaagtgtt tgattcagca taaaaatcaa
    48181 gttttaagta gagagcagat attggaagaa atttcaaaag atgtagctga tttaccttgt
    48241 gatagggtcg ttgatgtcta tattcgtact cttcgcaaaa aattagcttt agattgtatc
    48301 gtgactgtga aaaatgttgg gtataagatt agcttatgat aaaaaatcct aaattattaa
    48361 ccaagtcttt tttaagaagt tttgcaattc taggtggtgt tggtctagtc attcatatag
    48421 ctatttattt gacctttcct ttttattata ttcaactgga gggggaaaag tttaatgaga
    48481 gcgcaagagt gtttacggag tatttaaaga ctaagacatc tgatgaaatt ccaagcttac
    48541 tccagtctta ttcaaagtcc ttgaccatat ctgctcacct taaaagagat attgtagata
    48601 agcggctccc tcttgtgcat gacttggata ttaaagatgg aaagctatca aattatatcg
    48661 tgatgttaga tatgtctgtt agtacagcag atggtaaaca ggtaaccgtg caatttgttc
    48721 acggggtaga tgtctacaaa gaagcaaaga atattttgct tttgtatctc ccatatacat
    48781 ttttggttac aattgctttt tcctttgttt tttcttattt ttatactaaa cgcttgctca
    48841 atcctctttt ttacatttca gaagtgacta gtaaaatgca agatttggat gacaatattc
    48901 gttttgatga aagtaggaaa gatgaagttg gtgaagttgg aaaacagatt aatggtatgt
    48961 atgagcactt gttgaaggtt attcatgagt tggaaagtcg taatgagcaa attgtaaaat
    49021 tgcaaaatca aaaggtttcc tttgtccgcg gagcatcaca tgagttgaaa acccctttag
    49081 ccagtcttag aattatccta gagaatatgc agcataatat tggagattac aaagatcatc
    49141 caaaatatat tgcaaagagt ataaataaga ttgaccagat gagccactta ttggaagaag
    49201 tactggagtc ttctaaattc caagagtgga cagagtgtcg tgagaccttg actgttaagc
    49261 cagttttagt agatatttta tcacgttatc aagaattagc tcattcaata ggtgttacaa
    49321 ttgaaaatca attgacagat gctaccaggg tcgtcatgag tcttagggca ttggataagg
    49381 ttttgacaaa cctgattagt aatgcaatta aatattcaga taaaaatggg cgcgtaatca
    49441 tatccgagca agatggctat ctctctatca aaaatacatg tgcgcctcta agtgaccaag
    49501 aactagaaca tttatttgat atattctatc attctcaaat cgtgacagat aaggatgaaa
    49561 gttccggttt gggtctttac attgtgaata atattttaga aagctatcaa atggattata
    49621 gttttctccc ttatgaacac ggtatggaat ttaagattag cttgtagaca gattagtttt
    49681 ttattaaagt tcatataggg ttaacataag tgtgttattc tttgtgtaga taaaagaaag
    49741 gatactaata tggtattagc gattatttta gtaacattcc ttattcgatt gattttttta
    49801 aagcgttcga tagagaatga gaaacgaatc cttagcaatg gcgggaaaga atttggagtt
    49861 gagaatacaa aacgattaac tatggctcat attgtttttt atctctcttg cttggttgag
    49921 gcaatggtgc acaagacaat ttttgatagc atgggcatgg ttggtttagt cttgcttatt
    49981 ttttctatgc tgatgttaat gttggtgatt cacttgttgg gagatatttg gacagtgaag
    50041 cttatgcttg tcaataatca caaatatgta gatcatatct tgtttaggac agtaaaacat
    50101 cctaattact ttttaaatat tcttcctgag ttgattggct tgaccttgtt gagtcatgcc
    50161 tatatgactt ttgttttagt ttttccagtt tatgcagtta ttttgtatcg acgaatagct
    50221 gaagaggaaa agctattaca tgaagttata atcccaaatg gaagcataaa gagataaata
    50281 caaaattcga tttatataca gttcatattg aagtgatata gtaaggttaa agaaaaaata
    50341 tagaaggaaa taaacatgtt tgcatcaaaa agcgaaagaa aagtacatta ttcaattcgt
    50401 aaatttagta ttggagtagc tagtgtagta gttgctagct tgttcttagg aggagtagtt
    50461 catgcagaag aggttggtgg taggaatacc cccacggtta catctagtgg gcaagatata
    50521 tcgaagaagt atgctgatga agtcgagtcg catctaaaaa aaatattgag tgagatccaa
    50581 acacaattag atagaaaacg acataccgaa actgtagcgc taattaacga gttgcaaggc
    50641 attaagaaaa cgtatttgta taatttaaat gttttaaaag agaagtcgaa agttgagttg
    50701 ccgtcaaaaa taaaagcaaa gttagacgta gcttttgatc agtttaaaaa agatacattg
    50761 aaaccaggag aaaaggtagc agaagctcag aagaaagttg
//
LOCUS       DBERMR010000007        47897 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.007, whole genome shotgun sequence.
ACCESSION   DBERMR010000007 DBERMR010000000
VERSION     DBERMR010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 47897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47897
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.007"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(325..2052)
                     /gene="ezrA"
                     /locus_tag="ACL8A1_000481"
     CDS             complement(325..2052)
                     /gene="ezrA"
                     /locus_tag="ACL8A1_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HGS1201301.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2134..4080)
                     /gene="gyrB"
                     /locus_tag="ACL8A1_000482"
     CDS             complement(2134..4080)
                     /gene="gyrB"
                     /locus_tag="ACL8A1_000482"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HGS1201302.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEIVGDTDKTGTTVHFTPDPKIFTETTIFDFDKLNKRIQELAFLNRGLQISITDKREG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4095..4658)
                     /locus_tag="ACL8A1_000483"
     CDS             complement(4095..4658)
                     /locus_tag="ACL8A1_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGS1201303.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEVISNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4743..5297)
                     /locus_tag="ACL8A1_000484"
     CDS             complement(4743..5297)
                     /locus_tag="ACL8A1_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1 family glyoxalase III"
                     /protein_id="HGS1201304.1"
                     /translation="MVKVAVILAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYQDVFGKNQ"
     gene            complement(5308..6531)
                     /locus_tag="ACL8A1_000485"
     CDS             complement(5308..6531)
                     /locus_tag="ACL8A1_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HGS1201305.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6568..9018)
                     /locus_tag="ACL8A1_000486"
     CDS             complement(6568..9018)
                     /locus_tag="ACL8A1_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HGS1201306.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLELRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDDPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SHSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRMLESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYARELAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9218..10147)
                     /locus_tag="ACL8A1_000487"
     CDS             complement(9218..10147)
                     /locus_tag="ACL8A1_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HGS1201307.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELLVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARSLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVK"
     gene            10287..10574
                     /locus_tag="ACL8A1_000488"
     CDS             10287..10574
                     /locus_tag="ACL8A1_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HGS1201308.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10608..11942)
                     /gene="ciaH"
                     /locus_tag="ACL8A1_000489"
     CDS             complement(10608..11942)
                     /gene="ciaH"
                     /locus_tag="ACL8A1_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HGS1201309.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(11932..12606)
                     /gene="ciaR"
                     /locus_tag="ACL8A1_000490"
     CDS             complement(11932..12606)
                     /gene="ciaR"
                     /locus_tag="ACL8A1_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HGS1201310.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(12715..15261)
                     /locus_tag="ACL8A1_000491"
     CDS             complement(12715..15261)
                     /locus_tag="ACL8A1_000491"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HGS1201311.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEARQVASQIFATHKENLAELPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLTAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15384..15824)
                     /locus_tag="ACL8A1_000492"
     CDS             complement(15384..15824)
                     /locus_tag="ACL8A1_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HGS1201312.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDIILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTSESKVVLEEFRKVYPL"
     gene            complement(15799..16668)
                     /locus_tag="ACL8A1_000493"
     CDS             complement(15799..16668)
                     /locus_tag="ACL8A1_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HGS1201313.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFSELTNTEANCLVGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSVIFPFVNDDDQLKQAITVLRQQGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKIDFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(16646..17146)
                     /locus_tag="ACL8A1_000494"
     CDS             complement(16646..17146)
                     /locus_tag="ACL8A1_000494"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HGS1201314.1"
                     /translation="MEIKKHFGVYCVCFENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETRFTVRSYSNPRIYDVFVREELKNFMVHHVMALYDVEMNESAPQV
                     TTSEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVND
                     EKSTCP"
     gene            complement(17157..17855)
                     /locus_tag="ACL8A1_000495"
     CDS             complement(17157..17855)
                     /locus_tag="ACL8A1_000495"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HGS1201315.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(17848..18084)
                     /locus_tag="ACL8A1_000496"
     CDS             complement(17848..18084)
                     /locus_tag="ACL8A1_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018367459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HGS1201316.1"
                     /translation="MTFEEILPGLKTKKKYVRTSWGGAENYVQLFDTIEQNGLALEVT
                     PYFLINVSGEGEGFSMWSPTACDVLATDWVEVHD"
     gene            complement(18184..19110)
                     /locus_tag="ACL8A1_000497"
     CDS             complement(18184..19110)
                     /locus_tag="ACL8A1_000497"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HGS1201317.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFDLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCKLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(19185..20000)
                     /locus_tag="ACL8A1_000498"
     CDS             complement(19185..20000)
                     /locus_tag="ACL8A1_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201318.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(20004..20324)
                     /locus_tag="ACL8A1_000499"
     CDS             complement(20004..20324)
                     /locus_tag="ACL8A1_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HGS1201319.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            complement(20414..20605)
                     /locus_tag="ACL8A1_000500"
     CDS             complement(20414..20605)
                     /locus_tag="ACL8A1_000500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201320.1"
                     /translation="MKIKEQTRKLAAGCSKHSFEVVDETDEVSSKHGFEVVDETDEVS
                     NPTYGRATLTWFEEIFEEY"
     gene            complement(20655..22652)
                     /gene="metG"
                     /locus_tag="ACL8A1_000501"
     CDS             complement(20655..22652)
                     /gene="metG"
                     /locus_tag="ACL8A1_000501"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HGS1201321.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFCLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            complement(22776..24035)
                     /locus_tag="ACL8A1_000502"
     CDS             complement(22776..24035)
                     /locus_tag="ACL8A1_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1201322.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNVDEIANIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(24037..24738)
                     /locus_tag="ACL8A1_000503"
     CDS             complement(24037..24738)
                     /locus_tag="ACL8A1_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1201323.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(24722..25921)
                     /locus_tag="ACL8A1_000504"
     CDS             complement(24722..25921)
                     /locus_tag="ACL8A1_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HGS1201324.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PTHLVVANEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAQAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            26207..27553
                     /gene="gor"
                     /locus_tag="ACL8A1_000505"
     CDS             26207..27553
                     /gene="gor"
                     /locus_tag="ACL8A1_000505"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HGS1201325.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFNFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESVAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTSSEEFVTMR"
     gene            27749..28285
                     /locus_tag="ACL8A1_000506"
     CDS             27749..28285
                     /locus_tag="ACL8A1_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HGS1201326.1"
                     /translation="MKKAHVYAIPAIGAALIAVLAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLYFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQTYFTN"
     gene            28347..28493
                     /locus_tag="ACL8A1_000507"
     CDS             28347..28493
                     /locus_tag="ACL8A1_000507"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201327.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDETDEVSNHTYGKAKLMRFEEI
                     FEEY"
     gene            complement(28490..28705)
                     /locus_tag="ACL8A1_000508"
     CDS             complement(28490..28705)
                     /locus_tag="ACL8A1_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HGS1201328.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            28803..29123
                     /locus_tag="ACL8A1_000509"
     CDS             28803..29123
                     /locus_tag="ACL8A1_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201329.1"
                     /translation="MRHKFQQVLDKIHDFLNGHDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFSKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(29153..29491)
                     /locus_tag="ACL8A1_000510"
     CDS             complement(29153..29491)
                     /locus_tag="ACL8A1_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HGS1201330.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(29503..30222)
                     /gene="trmD"
                     /locus_tag="ACL8A1_000511"
     CDS             complement(29503..30222)
                     /gene="trmD"
                     /locus_tag="ACL8A1_000511"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HGS1201331.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(30212..30730)
                     /gene="rimM"
                     /locus_tag="ACL8A1_000512"
     CDS             complement(30212..30730)
                     /gene="rimM"
                     /locus_tag="ACL8A1_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HGS1201332.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            complement(30761..31549)
                     /locus_tag="ACL8A1_000513"
     CDS             complement(30761..31549)
                     /locus_tag="ACL8A1_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGS1201333.1"
                     /translation="MKRFEVSTEIGSLSVAYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIYDQAGKNLDNLADVVYEALEELGISEYILCVHSWSGI
                     LACKLLEKPIKCQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFENLSIESHVPVFIFCQT
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELSE"
     gene            complement(31653..31938)
                     /locus_tag="ACL8A1_000514"
                     /pseudo
     CDS             complement(31653..31938)
                     /locus_tag="ACL8A1_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654120.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
     gene            complement(32126..32365)
                     /gene="kphA"
                     /locus_tag="ACL8A1_000515"
     CDS             complement(32126..32365)
                     /gene="kphA"
                     /locus_tag="ACL8A1_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein KphA"
                     /protein_id="HGS1201334.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            complement(32385..32657)
                     /gene="rpsP"
                     /locus_tag="ACL8A1_000516"
     CDS             complement(32385..32657)
                     /gene="rpsP"
                     /locus_tag="ACL8A1_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HGS1201335.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            32827..33036
                     /locus_tag="ACL8A1_000517"
     CDS             32827..33036
                     /locus_tag="ACL8A1_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201336.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTTET"
     gene            33515..33727
                     /locus_tag="ACL8A1_000518"
     CDS             33515..33727
                     /locus_tag="ACL8A1_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201337.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEDFQDDDEIPTII"
     gene            33811..34614
                     /locus_tag="ACL8A1_000519"
     CDS             33811..34614
                     /locus_tag="ACL8A1_000519"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGS1201338.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIGGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(34671..36212)
                     /locus_tag="ACL8A1_000520"
     CDS             complement(34671..36212)
                     /locus_tag="ACL8A1_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HGS1201339.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMTEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGIMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(36214..36687)
                     /locus_tag="ACL8A1_000521"
     CDS             complement(36214..36687)
                     /locus_tag="ACL8A1_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HGS1201340.1"
                     /translation="MTKKRELILRLGVAVYSLCIVYFCFTPQATVEVGTPGIQHLGRL
                     VFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKKVLL
                     FSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN"
     gene            complement(36689..37774)
                     /gene="rlmN"
                     /locus_tag="ACL8A1_000522"
     CDS             complement(36689..37774)
                     /gene="rlmN"
                     /locus_tag="ACL8A1_000522"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HGS1201341.1"
                     /translation="MKPSIHSLTHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQGIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFAERGQDERVNHIVVM
                     GIGEPFDNYNNVLNFFRTINDDKGMAIGARHITVSTSGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALE
                     LAELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGGNCVVRQEHGT
                     DIDAACGQLRFNTMKRDRQKAVAAVNT"
     gene            complement(37795..38325)
                     /locus_tag="ACL8A1_000523"
     CDS             complement(37795..38325)
                     /locus_tag="ACL8A1_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HGS1201342.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKGQRHFVIRQK"
     gene            complement(38482..39087)
                     /gene="sodA"
                     /locus_tag="ACL8A1_000524"
     CDS             complement(38482..39087)
                     /gene="sodA"
                     /locus_tag="ACL8A1_000524"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase SodA"
                     /protein_id="HGS1201343.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            complement(39260..40297)
                     /gene="holA"
                     /locus_tag="ACL8A1_000525"
     CDS             complement(39260..40297)
                     /gene="holA"
                     /locus_tag="ACL8A1_000525"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HGS1201344.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQMLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKADSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            complement(40331..41266)
                     /locus_tag="ACL8A1_000526"
     CDS             complement(40331..41266)
                     /locus_tag="ACL8A1_000526"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HGS1201345.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            complement(41828..43018)
                     /gene="metK"
                     /locus_tag="ACL8A1_000527"
     CDS             complement(41828..43018)
                     /gene="metK"
                     /locus_tag="ACL8A1_000527"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HGS1201346.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            complement(43233..44576)
                     /locus_tag="ACL8A1_000528"
     CDS             complement(43233..44576)
                     /locus_tag="ACL8A1_000528"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGS1201347.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLESNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            complement(44716..45531)
                     /locus_tag="ACL8A1_000529"
     CDS             complement(44716..45531)
                     /locus_tag="ACL8A1_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGS1201348.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASLLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAILNWK"
     gene            complement(45583..47763)
                     /locus_tag="ACL8A1_000530"
     CDS             complement(45583..47763)
                     /locus_tag="ACL8A1_000530"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HGS1201349.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFTPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGTAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVGLEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
BASE COUNT        14196 a        10475 c         8647 g        14579 t
ORIGIN      
        1 agatttttca atagacttcg ttattggacg gttaccaagt tactcaaagt acagatttga
       61 agctgcagat aagactgacg aaatcacttt aatactaaat gaaaatcaaa aagcaaacta
      121 agagcctcag gctgtacttg agtacggtaa ggcgacgctg acatgatttg aagagatttt
      181 cgaagagtat aaaacactgt tttgaggtgg tggataaatc ggctgggcaa aaactcaatc
      241 tcaggagaaa atgaagtaaa tctccccaca actatagaaa aagatttgat tctgctcctc
      301 acacaataaa atctttttct tttattaaaa acgaatcgtt tcacgtgttt tctcatatga
      361 ggtaacaaag cgattggtta caccaggctc tgccacttcc aatgcttgag aaatcttgtc
      421 aaatgaagcg tgataatcaa attctttttc aaaaatatct aaagcttcgt taaatgcttc
      481 ttgaatgcgt tcatcaaatg agcgatagcg gttagaatat tgcaagagtt gctctgtcaa
      541 agttgcatat tgtacaatat tataagtttc cgtttctaaa gcttccatat cattcgttgc
      601 aatttcaaga actcgggtaa cagattcaat gttaatcatt ttttgttcta actcaaccat
      661 taaatcctcg gtattattgc ttgccgtaaa gaataacttc aagaaagttt gtggaatacc
      721 tggcagattg cgtttttcca tgtatcgctt gatagtatgg agacgattga cataaacatt
      781 ggccttttga cgtgcattaa tatcatcttt ctcaatttgt gtcaggcgct cactaactga
      841 aatttgctca tcttcaatat cttttagttg agtttgtaaa tcctcaagat tttcttcaag
      901 aactgaataa gcttgggttg gttcttcttg atttgaagtt acctcaacaa tagctgcctc
      961 aaaactctct aattctgtct gaatacgacg aacatggctt gcagctgtct caggaagtaa
     1021 ataggtcttg ttcaaacgtg caatatcttc tcccaataaa gtattattct ctttcatatg
     1081 ttgaaggtaa gttggaagag ttgcaagtag attttccact actttctgag cagcaatttc
     1141 tcgagtaaaa atatcataca aggcattgat ttcctcttgt gcctgtccat tctcatattc
     1201 ggcattatcc aattccaact gacgaatatt ctcttggttt ttcttgaatg cttcataaag
     1261 caagtggaaa cgcgcttcaa tatccgtttc aacaaaatga taattagcat caattagttt
     1321 acgataaccg gcttccaaat cctgtaattg atctggcaat tctgtagaaa gcgtcgtaac
     1381 caaggctgga acacgatcca caatatgact taaggccaaa atgtgatttt ctgtattatc
     1441 caaaatcact gcggcttcca cagggtcacc cgatgaattc aaggttacaa attgtgaaaa
     1501 ttcagattgg atattttcta attgtttttc aatttcatcc aaggcttgac catactgttc
     1561 tgaattttca gcaactctat gctgaagttc ctcaaataaa tccaaagcat gaagaacacg
     1621 accactattt ttagattctt gcttctctaa gtctgccaaa gcattgcgaa ttgccgcaat
     1681 atcttcttca atcaaagtaa tttgactctc aatttggtca atttgatgac tggccttgag
     1741 aaaacgaaat gaatggttat aaccttctgc ttcaaagaga ttattttcaa tatcggcaaa
     1801 agagttgaga gataaatcga cccatttttg attccattca cggaaagcca cttgactttg
     1861 tccaatcaag tgcatatttt ttacagcttc tacttcatca tttactggaa gattgtatag
     1921 ttcttctttt ctttcttcta gcgcctctaa tctcccctcg tttcgcttac gtagaaagat
     1981 tgccactaca taagccagaa ctaaaatgac tgcaattgca accattaaat aaattagttg
     2041 tccattagac atatcaaact ccttttttac ttgaaacaat cgtaacgatt atatcatatt
     2101 ttttagacca agggaactac ttctccctat tttttagaca tcaagtgtac tatagacagc
     2161 attttcttcg ataaactcac gacgaggctc tactcgatcc cccatcaaca tatcaaagat
     2221 tttatctgct tctgcagcat catctacaga aactctagcc atcaagcgat gttcgggatc
     2281 catggttgtt tcccacagct gatgatcgtc catttcacct agccccttat aacgctgaat
     2341 agtcggtttg gtacgacctt cactataacg ggctaaagct tcttggagtt tgatttcttg
     2401 atctgcaccc ggctggatat attctttaat ctcgcttcca accttgacac catagattgg
     2461 tggttgggca atataaacat aaccagcttc taggattggt ttcatataac gataaatcaa
     2521 ggttaaaaga agggtacgaa tgtgggctcc atcgacatcg gcatcggtca tcaaaacgag
     2581 tttttggtaa cgggctttcg aaacatcaaa ttctgcgcca aatcctgttc ccatggctgt
     2641 gaaaagacta cgaatttctt cgttagctag aatcttatcc atacttgctt tttcaacgtt
     2701 caaaatctta ccgcgaattg gaaggatagc ctgaaactca cggttacgac cagatttggc
     2761 tgatccacca gctgagtctc cttcgacgat gaagagttct gtttcagcag ggttattaga
     2821 agaacagtct gctagtttcc ctggaaggtt ggaaatttcc aaaccagatt ttttacgtgt
     2881 gacttcacgc gcacgcttgg cagccacacg agccttggca gctaaaatcc ctttttccac
     2941 gatacgtttg gcaatctgtg gattttccat gaggaaatcg gagaaggctt cactgaagag
     3001 gcgattggta atcttgacca cttcgctatt tcccaatttg gtcttggttt gtccttcaaa
     3061 ctgtggattt gggtgtttaa ctgagataac tgcagttaag ccttcgcgaa catcttcccc
     3121 tgttaggttg tcttcattgt ctttcagtaa cttattttta cgagcataat cattgataac
     3181 acgtgtcaag gctgtacgga aaccttgttc atgcgttcca ccttcatgtg tatgaatatt
     3241 attggcgaaa ctcatgacat tttcatggta accagttgtg tactgcatgg ctacctcaac
     3301 tgtgatatca tccatctcac cgtctgtata gattggtgta tcaaagatta catccttgtt
     3361 ctcgttgata tattcaacgt aactagcaat cccaccttca taatgataat gcttggtttg
     3421 ttccaaacct tcacgcttat ctgtaattga aatttgaaga ccgcgattta gaaaggccaa
     3481 ctcttgaatc cgtttattta atttatcaaa atcaaagatt gttgtttcag tgaagatttt
     3541 tgggtccggt gtgaagtgaa cagttgttcc tgttttatcc gtatctccaa ctatttcaag
     3601 atctgcgaca acatgaccac gacggtattc ttggtaatga atcttaccat ttttgtgaac
     3661 atgaacgtct aattgagtgg aaagggcatt aactactgac gaccccaccc cgtgaagacc
     3721 acctgaaacc ttgtatccac caccgccgaa ctttcctcca gcgtgaagga ctgtaaagac
     3781 ggtctcaaca gcaggacggc ctgttttttc ctgaatatcg actgggatac cacgcccatc
     3841 atccacaaca gtaatcgaat catctggctc aataaaaact tgaatatggc tggcaaatcc
     3901 tgccaaggcc tcgtcaattg agttatcaac aatttcccag actagatggt gaagaccttc
     3961 ttttgaggtt gatccaatgt acatccctgg acgcatacga acagcctcta agccctctaa
     4021 aacttgaatt tgactggcat cataatcctg tgcctgcaga tttttgattt cttctgtcat
     4081 cttattcctt tttcttatat gtctaatact tgtcttaaat tcacgatact ggtaaacaag
     4141 tgggtatcaa gtcctgcagc ttgacctgct tcgatatcaa tcggccgatc accaatgaca
     4201 agaccagagc taatctgata cttttctctt aaataaagca tggattcggg atttggcttt
     4261 ctcttaaagc ctgagctaga agtcaccact tctgtaaaat aagctgctat agaggttttt
     4321 tctaaaattt ccaaaacctg atcatttcga tgagagacca aaaaatgacg gccaccttga
     4381 tttgaaatga cttccaatag gtcagaaact ccttcaaata aaatcgggtg ttcaagctct
     4441 ctggcttcat tttccttgta cttttctaaa aaattctcta aattgggagc gaatgtctca
     4501 atcgcaaaag gagtagaaac ctttaaagct tgatagacac tgtcatggtc ttgtgtgata
     4561 ccatacagtg ccaatgtttc aacaaatgca gctgttgaag tttcataatt atccagtaaa
     4621 gttccaccta aatcccagat gtaatcgtga tatttcatac ccttcattat agcatttttt
     4681 tatgaaaaaa gcgatgattt ccctgagttt tccacataaa aaacgagaga ataactcccg
     4741 ttttactgat ttttaccaaa gacatcttga tagagcattc ctgttcgtaa actctctgcg
     4801 tcccctccta gttgctccac caactcgtag gcaaaggcaa gggctgttga aggaccccga
     4861 ctggttgtca actgaccatc taccactact gtttccttga catagtgacc atcaaggatt
     4921 tgctcttgaa cgccgtcata acaagtgtat cgcttatttt tcaatatctc tgcttgattg
     4981 agggcaattg gtgccgcaca aatggctgct agtttcttcc cttcttgctc gaagctttgc
     5041 aattcttgaa tcaaggtctg attatcacgt aaatgtgcag aaccaggcat acctccagga
     5101 agaacaatca tatcatagtc tgataaatct ccatcaaaga catgatctgc tcttacttgg
     5161 attgcatgcg aacccgttac ttgctcttca aaaccaacca tatcacatgt gatattggct
     5221 cgacgcaaga catctacaac tgtcaaggct tcaatttctt caaagccctg agctaatata
     5281 actgctactt ttaccatgat tttctcctta tttaatttgt tttaatacaa cctttttccg
     5341 tttgaatgga acttttccgc tcttttcttc agctagatta gtctggtaac tcatcgataa
     5401 aagcaaacca acaccaatca gattactgat aatagctgat cccccttgcg aaatgaaagg
     5461 caagggaatc cccgtcaaag gaagtagtcc agtcacagca ccgatattct caaagatgtg
     5521 gaagagcaac atcataatca aacctgtgga aatataagtg tagaactggt tatttgattt
     5581 aagagtaatc ttcaacatac ggtaaatcaa catgagatag agggcaataa ccaggacaga
     5641 gccaataaag ccaaaatctt ctgcaataac cgtaaaaatc atatctgact ctcgaactgg
     5701 gataagcaga ttcgaagcat taaatccctg accaaataag ccaccactcc caatggcaat
     5761 ctgcccttga gcctgctggt aagtcgttgt ttgggcaaac tcaaagggat tgagccaagc
     5821 caaaatccga ttgatttggt aggtcggcat tccaatctgg tgaagaaaag ctcgtccgtc
     5881 cttgctaata aagatagcta agaaaccagc aactcctgtt acagcagtca caaatactgg
     5941 gataataatt ttccaagaaa cccctgataa taaaacgatt cctgagaaaa tggctacaaa
     6001 aaccaaagcc gtccccaagt cactttgaag tgctaaaaga actaggactg gaatggtaaa
     6061 gagaatcatc cagaaaatta acaaaaagtc cagcggaacc gtgcgtctcc attccttatg
     6121 tttctttgta aattggacaa tgacacgagc caacatgagg atataggata tcttcataaa
     6181 ttctgacggt tggaataggg taattccatt tattgatacc cagtttttgg cacccgttga
     6241 tgcaactaag cttggattat aaaatacaat cggcaagatc ataagtccca agcctaaaat
     6301 atatagaaag ggggtcacct tccaaagaaa ttctgtatta aagagcatga ccacaaaacc
     6361 aatcacaagc cccaaggcaa tccaggcgac ctgctgccct aaaatgggca gaatattatt
     6421 ggggtaatca tgactaacgg ctatatagat agccaccaca ccgatgacca gtagaaaaaa
     6481 tactggcaag agcaaactgt aatcgactct tgagtcgaga gaacgtttca tatatactaa
     6541 ccttatactt tcatacaata ctatttatca aagttcatta aaaaatctat caatagcctc
     6601 gtcaacttcg gatcgagaga tggttttaac agtcgcttct tctgctagag atgctactat
     6661 ttgtttgccg tatcgttttc cgacgattct cctatccaaa ataagagtta aggaacgttg
     6721 gtattcacgt ctcatacttc ttcccaaagc ctgttttaaa cgaataatgg ccattggcaa
     6781 ttgataatca taaaaggcat ttttcccttc ttgattcagt tcttgattaa tctttttcgt
     6841 caagggttct tgaggatttt ggaaaggaag cctcggtaca acttgaatca caaaaggatg
     6901 gcttgaaaaa tcaactccct cccagaaact tgctgcacca agcaagattt gttgttcacc
     6961 tttttcaaag cgtttcttta gctgatgaac atccccattt ttatactggg ccaagtggct
     7021 aactgtaagt aaatccgata ctgctagaag catgtcttta gcggtaaaga gaaccaaaat
     7081 cggttgctgg aaagcttgaa tttccactag taaagcagct aactctctgg catagacttc
     7141 taaggaggtt tctgttacca gagggaaatc tttgaccatg accacttctt gttcctgttt
     7201 tccccgagat tcaatcttga caaatttagc ttcaggatag cctaaaaggt ctgccaaaga
     7261 aaccctctga ctaatctcaa gagtagccga tactcccagg acttggcaac tctcaggaag
     7321 taaagaggac agaagagtac ggcctgattt tgtagaagaa atctgaattt tcttttgact
     7381 cgtttcagtt acttcaagcc agtaatcacc ttcagctgta aaatagcgaa ccagctcatc
     7441 aaagccttct acttctaatt ctgaaaaata ctgatggaga ttatccagag aatctaagat
     7501 atttttccta gatttgccag actgaaattg ttctatcaag tagagacact caaagcgaat
     7561 actttctagc atccgttgtt gaaccctgtt ttcttctcct actaaagcct tatcaattaa
     7621 atcgataata gattgtatat cgtaggtctc ttgaagcaga ttttctagag ctaacaaaat
     7681 cttttggact tcatcaataa tcagtaaacg gtcactgaca aattcaggat tatcttcaag
     7741 tctggtcacg agataggcat gattagtcac taaaagcttg caagtctctg ccctttcttg
     7801 actacgtttc caaaaatctt ccgtcacaaa taagctatgg gatgataaat tcccatcatg
     7861 acgaaggtct gctagaaaat gttggtaacg gtagagttgc ccgatttcat ccaaatctcc
     7921 tgtctctgtc tcagtaagcc agaccaagac ttgcatttta aagcgtctaa ataagcgatt
     7981 ttcatcattt tcctgcaagg aatgataaaa ggcatccaac ttcagataat tttgtggtcc
     8041 ctttaagcta tgaatatctg tatggaacac ttccttgaga cgtttacctt cttcttccat
     8101 gatttgattt tgaagaatct ttgtcggaac actaagaaca atttgtcgct ctttggattg
     8161 agataaagcg ggtaagagat agccataggt tttcccaatc cctgtcggcg cttgaatcag
     8221 agagacaggg tcatctttca atagcaagcc aacctcttta gcaaaacttt cttgttcctc
     8281 cctcacttca aggttcaaca gagaaatatt tttagaaaag tcttgagata gttttcgtag
     8341 ctccagagaa gctgccgttt tcttaaaata cagaccttga acttggacca agtctggaga
     8401 actcaggata gattggttgc gataagtttc ctcaataacc aggtaggact catataggag
     8461 agcgtcagcc atttccagca agcgttccaa gagaccttta ggaagctggg tcatcttttc
     8521 ccgtaaaaaa agaagtaatt ctgctgtagc ttgggcatct gaaagggctg tgtgtgcgtg
     8581 tttaagagga attcctaatt ctcgacacaa aatcggcaag ctatattttt ccagttcagg
     8641 gaaaaagacc tgggccaatt cgaccgtatc aacacgaggg tttcttagct catagccttc
     8701 aaaaaataaa ttttccgcca agagattagc atcaaactga acattatggg ctacaaaaat
     8761 cccatcctcc accaagtcaa agatttttct ggcaacttgc gaaaaatcag gtgcttgcgc
     8821 cagacgttgg tctgtcaatc ctgtcagttc tttgatatga gcatccaagg gttcatgtgg
     8881 attgacatcc gtcgtatagt gatcgacgat ttctccatcc tcaatcacga caattcccac
     8941 ttggataatt ttagccttac tacctgtgct agttgcctct aaatccacca caacatagcg
     9001 attaccaatc gttttcatta taaaaaatta taccaaaaaa agggccattt ggcacctgca
     9061 aacataggat aattttcaaa ctcaagaaca ctacttttct tagaaatccc atcgaataga
     9121 gcattctagc ctgctgattt ttttaaaata gtgaagaaat acgaaaaatt gagagaagaa
     9181 atgttttcat cttccttctc tcaacgaatc atgtatttta tttaaccatg tcaggatcgt
     9241 agtcataaaa tcctgtatca aggaaacggt ttttagggtg aagtttacga acttcttcat
     9301 ccagcaagaa ggcttggtca tggctaaagt tgccctcttg aatcaagcta cgcgcttgga
     9361 taaagagttt tgcaatctca acaaagttct gaccaggagt gtagagccct tctagtttcc
     9421 agcgagtaaa gccatgctcc accaattctg ttaatttgat catcaaatca aggtcattgt
     9481 tggcaaagat atgggtccca tgattatctt caaaaatgga atagtggctc tctggatcac
     9541 ttggctcagc caagaagagg tcacgtttat gcgtcttttc atcatcgata tgtgtaaagt
     9601 tatagtagtt ttgcaagagt ggacgtttag aatgatggat gacgctagca ccgtaaacca
     9661 aaacttcagc aggaatttcc aaaatctctg gcattttgaa aagttcagct gatggaattt
     9721 cacgcgccaa aacagcctca gatgcgccag ccttttgtcc ccagaagtta atctgacgac
     9781 tgctagttac catggttgaa gcatcgtaga tggtcttaaa tgaataacca tcgcggttaa
     9841 ctacgtaaaa gacgcctgca tccccaatcg taatatagtc tgtcttgatt tcttccaaga
     9901 agtctaagaa aggcttgata cggtccatca tatcttggtg catgagagca ttgaccgcaa
     9961 cgagcaattc cttaccagca tcatgaacca acttagcgat ttcacgtaat tggtcataac
    10021 taaaggtcgt tggcagacga aggccaaaat ctttctcacc gacatagata cggtctacgc
    10081 cagcttcgag tagttgttca acttgttcaa tactttcagc agttgctgta atgacaatct
    10141 tttccataag aaaaattata ccacatttct ccaaaatcat ccatgctttg aaaatgaaaa
    10201 gggactttat tctttttgta acctttctgt aataattttc tgccgaaaaa atgataaaat
    10261 aggtatgtac tgttaaggag agaatcatgc ccgtaagaaa attacaatcc tatgaggtag
    10321 actatcaaga agaattaaac cagcagcttc ctcattacca agcttataca cctgaagcgc
    10381 aatctgatgc caatctcaag gaaatcctat tttttattaa tatcgctgtt ttttgtatct
    10441 gtattgctat ctttagtttt atctttttag cattaaaatt atcaactgct cttgcctttg
    10501 ccgcagcaat cggattcagc ttacttgttt taaaagttca acggtctatt atcaaacgaa
    10561 aacgtagaag ataaatccaa aatatcgccc cagttggagc gatattttta ttttttcttt
    10621 ttagatggtg tctgaatggc aatcttcact tcaaagattg ttcccttggg tttattatct
    10681 ttgacagtaa cagttccttt tagagcatct acaatttgct tggctaggga taatcctaaa
    10741 ccaaaaccac ctttttgccg ggttctagct ttgtctactc gataaaaacg gtcaaaaatt
    10801 ttctttttat cttctgtcga aataccgatt ccattatcag aaacaagtaa ataaagattg
    10861 cgatcggtcg ccgagataag aaaatcaatt tcaccatcct cctcagtata cttgacggca
    10921 ttatcgaaaa gaatggtcat cagttgtttc agaagaagct gatctgtgac aattgttcga
    10981 tggatacgat tttcaaaacg gaagacacga ttattttccg aagcaatcat ctcgtagttt
    11041 gtgaaagttg tattaaaaaa gctagttgga acttctgcaa gctccggctt aatcccatca
    11101 tctctccgag ctaagttcag caagcttgtc gttaaaaaac gcatatttcg gacttcttcc
    11161 aaactcgatg caatgctttc gctcacatcc ataatggtag cttctggctt acgaaaaagg
    11221 gtctctaagc gattttgcaa aactgcgagt ggagttcgta actcatgact ggcattttcc
    11281 acaaaagact gttgcttctg catactctca agcaggggcc taacactgac cctagctaga
    11341 tagagactgg caagtaaaga caaaatccag aaactagcca tcacgaccac aatcaattgc
    11401 tcatgctttt gactagcctg ctccaactga ctggtattaa tcaagacagc agcatacttg
    11461 atattggttg aaaccgaact aatattggtc tccatcaaaa tcacacgata gatttcttcc
    11521 tgcccatagc tattaaaaac ctgaatctgg tagatatgtc ctagttcttt cttttctaac
    11581 ttaatcttat ccaagcccaa aaatcgattt ccagaaagaa gttgagtaaa gtctttatca
    11641 aagagaatga cttccgtatt ggaactgaca ttaggtttta tttctacttt actagcgtcc
    11701 gccctagcat tttctaaatc tttaatctct tctgttgccc tatttatagc cagctgaata
    11761 actgcttgag gattttcact cagtccatga agcttatcgt ccaccgaagt ataaagactc
    11821 gaatgcatga cttgtaaaat aatcagagtc attgtagaaa aaatcagggt gaagacaccg
    11881 aagttgcgga taaaataact aaagtcatcc gcataccatg tttttttaag tttactgaac
    11941 atcttttaaa agatacccaa cactacgcaa ggtttgcaaa ttctctgcaa aagtggttcc
    12001 ctttaatttc ttacggactt ttgaaacata gacttcgaca accgaaatcg ttgtatcact
    12061 atcaaatccc catagacggt caaaaatctg cgtcttaggc aaaatcacat tttgattttg
    12121 aaggaaataa actagtaaat cgaactcttt ccccagcaat tcgacaggag tatcttcaac
    12181 tttaacggta ttggttgata aattaaccac gatattccca taagtcaagg tgttttcatt
    12241 aaacttccct gaacgtttga gaagggcctg aatccgcatt ttaagttctt ctaggtagaa
    12301 aggtttggtc agataatcat ccgctcccag ttcaaatcca tgtcccttgt catccaaact
    12361 ttccttggca gtcataatca gaactggtgt cgtaattccc ttttcacgca attcttttaa
    12421 gacttggaaa ccattttttt ctggcaacat caaatccagc aaaatcaagt catagacacc
    12481 actctcagct tcgtagagac cttcttctcc atcaaatacc tgcataacat ccgcaaaatc
    12541 gtctaaaaag tcaaatactg aatttgacag acctaggtca tcctcaacca ataagatttt
    12601 tatcatgaga aactcctcct tattaaaact attataccaa atttgcctta aaaaaaactc
    12661 aactctctgc attttacatg agatagctga gttttctttt tattttaggc ttatttatgc
    12721 atttccgtat tgaagaacaa ctgcttcgac tgcagctttt tcacggctaa tcaagtcaac
    12781 acgcgctgca atttccttga ttcccatacc gatgttacgg ctaagagcaa ggtcagaaag
    12841 ttgcggttca aagaactcct tgtattccgc caagcgttgc tgagtcttaa atacatgagc
    12901 aggaaggata acaaagctat caaagctcat atctcctcca agggctgcct taatccaagc
    12961 ccagttttca cgcgcccaag accaagctgt tttctgagtt gcttgatgag ctaggaattg
    13021 gtaataccaa gcagacaagt cctgtggttt gaccacaaat ttgtccttcc aagaagtaat
    13081 caagttttgg atattatccg catctgtact gtatgcaaga gctgctgtca actggcgttt
    13141 aaagacagca tctgttgcgt gagtataagt atcaagataa agtgctaaca agtctttagt
    13201 ctcatgatgt ttcatctcat taatcagaac ttgtgaacga atagctgctg ggagttctgc
    13261 aagattctcc ttgtgtgttg cgaagatttg gctagcgact tgacgagctt ctgcatcatt
    13321 tgagcgaatc atcatcgaaa cagccagctg acgaaccaat tcatcctcat ctgattctcc
    13381 gtctttagct tcaaaaccaa gacggtcata gttatgacga gccaatttag caaccagtcc
    13441 tttgaaggct gtttcagcat ccgttccttc atcaataaag cgctcaaggg ctgaaatcac
    13501 ttgagaaaca gctgaaacca ccagataaga ctcttcctta gcaagtttat caagaactgg
    13561 aagcaagtct gcataagaaa tgtgccctgc ctcagccaac aaacgacgtt cttgaacaat
    13621 ttgcagtttg cttgtgttat caagtgtctc tagctcagca agaacagctt ctaacaagtc
    13681 tccttgatag tcggtaatat agtgggcagt attttcagtg ttgagacgaa gagctccttc
    13741 attttcagca agaagagctg cgtagccagg gatttcgata ctttcagttt cgagtgtatc
    13801 aggcaagcct ttccagttgc tattgagggg caccacccag agacggttct tgtcttcgtt
    13861 ctcaccgatg aagaattgtt tttgtgaaat cttcaagaca tcattttcaa ctttgacagt
    13921 aagaactggg taaccaggct gttccaacca agaatccatg aaggctgcga catcacgtcc
    13981 tgacgcttga ccaagggcat cccaaaggtc actaccaatg gtgttgctgt attggtgttt
    14041 ttcaaagtag gcatgcaaac ctttagcaaa atcagcatct cctagccaac ggcgaagcat
    14101 gtgcatgaga cggcttcctt tggcatagac gatagcgccg tcaaagagtg tattgatttc
    14161 atctggatgt ttaacttcga cgtggacaga ctgaacgcca tcagtagcgt cacgttcaag
    14221 agcaagaggt actccacctg tttggaaatc ttcaaagata ttccagcttg gttcgatggt
    14281 atccacacag acgtattcca tcatattagc gaaactttca ttgagccaaa ggtcatccca
    14341 ccatttcata gtcacgaggt tcccaaacca ttggtgagcc aattcatggg ccacaacaag
    14401 ggcaacttgt tgacggctag caaatgtaga gttctcatcg acaaccaagt aaacttcacg
    14461 gtaggtcaca agaccccagt tttccatagc accagctgag aagtcaggaa gggcgatgtg
    14521 gagagattga ggaattggat acttaactcc atagtaatct tcgtaaaact cgatagagcg
    14581 aacagcgata tccagtgaga aatcaagatt tgaaagtgga tgtgctttgg ttgagtagac
    14641 acctaccagg gtaccatttt tagttttagc ggtcacccct tgcaaatcac cagcaacaaa
    14701 ggccaacaag taagaagaca tgcgaggtgt tgtctcaaac ttccagatac ctgtttcctt
    14761 acggttttca acatcgattt ctggcatgtt tgacaaggcc aattcacctt ctgcttggtc
    14821 aaagcgaaga gagaggtcaa aagttgcttt ggcttcaggc tcatccacac atgggaaagc
    14881 ttcgcgcgca aaatggctct cgaactgagt agacaagacc tccttcttga ctccatcaac
    14941 tgtataataa gaagggtaaa tccctgtcat gttgtctgta attttaccag aaaaggcaag
    15001 aaccaattca acttgaccag cctcagccaa ttcgatatga agggcttcat tgtcatggtc
    15061 aactgtaaat ggacgagctt gacctgcaac ttctacagag gtgatttcca agtctttttg
    15121 gtggagggag atgcggtcac tctgtgcttg accagtgatg gtcactttcc cagaaaaagt
    15181 cttggtctca cgactcaaat ctaaaaataa atcataatgt tcaggaacaa attgcttaat
    15241 aaaatgttca actgcttgca tagttttctc ctattctaag tttaagagct gctgctcttc
    15301 ttcaaacaaa cttattatat catattttgc ttgaaaaaaa aggattggac agcgatttcc
    15361 aaaactttct tccttctaat agtctacagt gggtagacct tgcgaaattc ttctaaaaca
    15421 accttgcttt cagatgtaaa agtcagccct acttcaccca aacagtctgt gaaaaagtcc
    15481 tcatgaacct ggcgccaata ggcaagtgat ttgtctccct caccttcctt aaaggcatgt
    15541 tgcgcagaaa cttgattgaa gagttcaaca gaaacctttg taatttcgac aatgcagaca
    15601 gactgatttt gactatctaa aatgatatcg aaggtcccta cttggggaag tggttcgtct
    15661 tctagtacat agaggtcata ggctgatgct gttgctgtct tttctccttt aaacaccaaa
    15721 tccgctaaaa ggtctggttc aactccaaaa gcccaagcat cgatttcatc tccgatcaaa
    15781 ggattgattt gcttgtattt attccacatt tcttgcggta tcatgggtac tcctttgtaa
    15841 ttttttactt tcttctttta tgtgtttaag atgatctgga tggtcaatct ctaaatcaaa
    15901 aatctctgga atagaactgt agtggataat gcacttgata cccaactgat tcattttttg
    15961 tatgaaagaa gtattcagat agcctgctac agcaaaatca atcttgttct ttcttgcttt
    16021 atcctgcata tctcttagca tatctaacat tattggactt tccatatcat gccattgact
    16081 gtttctcata gtcgcaaaaa caaaggaagt caaatcattc attccaacta caatctttga
    16141 aatgcccgtt tccagtatac tagataagtc aaaatacgct gacggtaatt caatcatcgt
    16201 tccgactttc ccagtaaaac cctgctgacg caatactgta atagcttgtt ttaattggtc
    16261 gtcatcattg acaaaaggaa agataacaga tagattgggg ttggtttgat aaacttctgt
    16321 aacgacatgt gcttcagcct gaaattcatc caaacacgcc agtaaacgcc tggttcctct
    16381 atagccaaac aagggatgcc cttcgtcaaa aaactctcta gtccccacta aacaattggc
    16441 ttctgtattc gttaattcag aaaaacgata ccaaacttct tcatctgagt acagggagca
    16501 gatagtctct agataatctt ttacaaattg ctgacaactt gctaatatga tgttttgatt
    16561 aagctctctc aacaaatatt ccccacgaat catgccgacg tggtgaaata gttgaggata
    16621 aattttttca agaatttttt cgccactaag ggcaagttga tttctcatca ttcaccttcc
    16681 aattcatgta agaagtcttg tccagttctg gaaatcctaa taattcagac ttaaccttca
    16741 agactaatgg cgatgcattt tcttctgtaa tttcttgaat atccatccaa atatatccaa
    16801 gtgaatcatt cgcaccatca gacacagctt ccgaagtcgt aacttgaggt gcactctcat
    16861 tcatttcaac atcatacaag gccatgacat ggtgaaccat aaaatttttt aactcttctc
    16921 tgacgaaaac atcgtagatt cgaggattag agtagcttct aacagtaaat ctcgtctctt
    16981 ccataacttc tctagtcagc gtttccgtca gtccttcacc aagttgctga ctgcctccag
    17041 gtagatcata ccgatgttga taagggcctc tcgttttttc aatgcaaagt aactttccat
    17101 tttcaaagca aacacagtag accccaaagt gttttttgat ttccatccaa ctcctcctac
    17161 ttcaaagacc agccaccatc tattgtcaag atttgtcctt gcatggcgct cgcttttcca
    17221 cttgctaaaa aaagactaag ctctgctatt tcctctggct caatccagcg cttgattggg
    17281 gtttcactag ccacccagtc agccaaacca cctggttcaa aatccgcagc ggtcatagct
    17341 gtcttgactg ctcctggagc gataccaaag acctgaatcc cagcttcagc atagtctaga
    17401 gccaactgct tggtgaagcc agccaaggca tgcttggatg aagtataggc gtgaccacct
    17461 ccacctgcta ggctagaagc aatggaacac atattgatga tgattccctt tttattttcc
    17521 agcatttgtg tcaaataata ccgagtcaac tctactggaa taatgtagtt gatttcaaaa
    17581 atctcttgaa tgtcctgcgc cgtttgttcc aacagtggtt tgtaatcatc caaaactccg
    17641 gcagtattac acaaaacatc cacctgaggg caccagtcaa aaataggttc caagtccaag
    17701 gtcaaatctc tctgtaaaaa gtgaaaatca ccctctaaga gtggcttttc accttggtca
    17761 actccataaa cttgatagcc cttctctaaa aagaggcgag cttgagccaa tccgatccct
    17821 gaactcactc ctgtaatgag tacacgttta gtcatgcact tctacccaat ccgttgccaa
    17881 aacatcacaa gctgtcgggc tccacatgga aaaaccttct ccttctccag acacgttgat
    17941 taggaaataa ggtgtcactt caagtgcaag cccattttgc tcgatggtat caaagagttg
    18001 gacatagttt tccgcacctc cccaactagt tcgtacatat tttttcttag tctttaagcc
    18061 aggtaaaatt tcttcaaatg tcatgttttt ctcctttaat gtcaataatt ctccccttca
    18121 ttataacaaa aaaaccgctt taaaacggct ttttgactgt gatttattca aacctgctac
    18181 tacttacggc aaattattcc ctgcagcaag ataaatttca taccattctt ttcttgttaa
    18241 gctaaagttt gccgctcggc taacttctct caagtgctta ggatttgttg tacctacgac
    18301 tgcctgcatt tttgctggat aacgcaatat ccaagaaatg gcaatagttg aagaggttac
    18361 tccatattta atagctaaac gatcaagtac ttgatttaaa gcttgaaatt tctcatttcc
    18421 aacaaagttc cctttaaaat acccgaattg caagacagac catgcttgaa tgaccacatc
    18481 gtgtaatttg caatattcaa aaatgctgcc atctcgcata gctgcttgac tatcttccat
    18541 attaacatga aaagctgatt caaatcctgg agtaaaagcc gcactcaatt gtagctgatt
    18601 aacagctaac ggctgcttga catctttttt aagcaactcc atcatcatag gattttgatt
    18661 agaaactcca aaatctcgaa ctttaccttg tttataaagg agatcaaagg cttctgctac
    18721 ttggtcagat tccatcaaag catctggtcg atgaaggagc aagctatcta gatgatcaat
    18781 cttcaatctt tgcaaaatac cgtctactga ttttataata tagtccttag aaaaatcaaa
    18841 ataggtaaat tcttcaatgc gaatgccaca tttggactga atccacatct tttctcttaa
    18901 atctggacga ttttttagga caagacctaa cagttcttca caacgaccac gaccataaat
    18961 atcagccaag tcgaaggcat tgattccaac agaaagtgct gtttctacaa gctcttcaac
    19021 ttcttttaca gacttatctt ttattctcat cattccgaga acaatttctg ataattcttt
    19081 gtcatcttga ccaagagtta tgtatctcat caaatttttc tcctttaatt tctaacattc
    19141 ttcccttcat tataacaaaa aaccgctttg caacggcttt ttgactatac ttcactccat
    19201 tttatcttct taaacccacg gaacaagaca aagattccaa taaagaggac agctaaagga
    19261 ataacttttg taaggaaaac atttgaaatt cccatccact cataataacg gagcagagaa
    19321 cccaccacaa gatgggcaat aatcatactg acaaatggac gaaagaccgc ttctttccaa
    19381 ttccaaatac cgataactag cgaaatcgta aagacagaca aactatccca gggagccgga
    19441 atataaaagg ctccttcttg tatgaagctt gccattccta catatcctaa aacaactaga
    19501 agaactatag tcccaacaac aatgtaagtg ccaattttca ttttaggaga atcttggact
    19561 aaacttcttc gtaaaattgt ggccacaagt ccaaatccaa tcagaaaaat aagaagttgc
    19621 cctaaaaatg tgagcaaatt gactgttaag agaggacctt tagaaaaatc acttagtagt
    19681 tgataataac gtaataccgc caggacaaga attggcgtca aaagggactc tttgatagaa
    19741 ctgcgaggtg ctcccttgag aatctctttc attatttttt taggattctt acctagataa
    19801 tcctctgcac tcatgccatc tcgttctgct tctgagaaat ctagcatcat caaatagatc
    19861 tgctctctga gatagtcttc atcatagaga aatccagcaa gattaaaact ttcccacaac
    19921 tcctcaaaat acttttgatt ctcctcagaa aactcatgta gcaaagcgct tgtttcttcg
    19981 taatacttca ttttcttcat ggtttaaccc ccattcttaa tcccttctac tttttgactc
    20041 aaatcgtccc attgttgcca aaagactgag acacgctctt ctccttcttt cattaatgaa
    20101 aaatacttcc gatctggacc atctggcgac gggcgcatgt cgcctcttat ccattgattt
    20161 ttttctaact tttgcaacaa aggataaata gttcctggaa cgatagtatc aaatccagcc
    20221 tctcgcaaag tctgaaccaa ctcataacca taccgctctt tttgaccaat catatccaag
    20281 acacaacctt caagaacacc ttttaatagc tgagtttctt tcatcacttc tcccttctaa
    20341 tctattttgt aatacctact agtaacttca cctatagtat atcacttcta cactagtttg
    20401 taaagcataa tagttaatac tcttcgaaaa tctcttcaaa ccacgtcagc gtcgccctac
    20461 cgtatgtagg gttactgact tcgtcagttt catctacaac ctcaaaacca tgttttgagc
    20521 tgacttcgtc agtttcatct acaacctcaa aactgtgttt tgagcaacct gcggctagct
    20581 tcctagtttg ctctttgatt ttcattgagt ataaataaaa aaacagaact agcctgaact
    20641 agtcctgtct acttttaccc aatcacactt ccatttggta cagctggatc aactgtgaga
    20701 agggttaatt tgccatcatg ttcagctgag agaatcatac cctggctgac atattttttc
    20761 atcattttac gtggtttgag gttagcaacg atttgaactt tcttgccgac caattcttgt
    20821 tcatttggat agtatttggc aattcctgaa agaatctgac gatcttctcc atcaccagca
    20881 tccaagcaga attgaagcaa cttatctgaa ccttctactt tagacacttc tttgacttct
    20941 gccacacgga tttcaacctt gtcaaagtct tcaaacttga tttcatcctt gtttagtttg
    21001 agctcaactt cgtccggatt ccattctttt tcgactgctg gtttattgcc ttccatttgt
    21061 tccttgatat aggcgatttc ttcttccata tttagacgtg gaaagatagg tgttcctttg
    21121 gcaactacag tcacatctgc tgggaagtca gccaaactca agttttcaag actagaaact
    21181 tcttccaaac caagttgagt caaaactgca cgactagttt ccatcataaa tggttcaatc
    21241 aagtgagcaa ctacacgaat gctggctgcc aagtggctca tgacacttgc caattggtca
    21301 cgaagagctt catccttggc caagacccat ggtgcggtct catcgatgta tttattggta
    21361 cgagagatca gagtccagac tgcttcaagc gcacgtggat agtcaactgc ttccatgtgt
    21421 gtatggaagt ctgcgattga ttgttctgca acctcagcaa gaacatgatc aaattcagtc
    21481 acaccttcta cataggcagg gatttgtcca tcaaagtact tattaatcat ggaaaccgta
    21541 cggttaagga ggttcccaag gtcattagcc aattcatagt tgatacggcc aacatagtct
    21601 tcaggagtaa aggttccgtc tgaaccaact ggaaggttac gcatgaggta gtaacgaagt
    21661 ggatctagtc cataacgctc taccaacatt tcagggtaaa cgacattccc ttttgactta
    21721 gacatttttc cgtctttcat gacaaaccaa ccatgggcaa tcaaacgatc aggtaattta
    21781 acatccaaca tcataagaag gattggccag tagatagagt ggaagcgaag gatatctttt
    21841 cctaccatat ggaagactgt tccattccag aacttgtcaa agttaccatg ttcgtcttga
    21901 gcgtagccaa gagctgtcgc atagttaaga agggcatcaa tccaaacgta gacaacgtgt
    21961 tttggatttg atgggacagg cactccccat gtaaaggttg tacgagatac cgccaaatct
    22021 tccaaacctg gctcgatgaa gttgcgtagc atttcattaa ggcgaccatc tggcgtgata
    22081 aattcaggat gagctttgaa aaattcgacc aaacggtctt ggtatttgct aaggcgaaga
    22141 aagtatgatt cttcagaaac ccattcaacc tcatgacctg atggagcaat accaccagtc
    22201 acatttccag cttcatcacg gaaaacttct gccagctggc tttctgtaaa gaattcttcg
    22261 tctgatactg aataccaacc agagtattca cccaagtaaa tatcatcttg agcaagtaag
    22321 cgttcaaaga cctgtgcgac aactttttca tggtagtcat cagttgtacg gataaattta
    22381 tcgtatgaga tatctagtaa ttgccagagt tctttaactc caaccgccat tccatcaaca
    22441 taggcttgag gtgtaatacc agcttcttcc gctttctgct ggattttctg accatgttca
    22501 tcaagacctg tcagataaaa tacatcgtag cccatcaggc gtttgtaacg tgctaggaca
    22561 tcacatgcga tagttgtgta ggcagaaccg atatgaagtt tcccagatgg atagtaaatc
    22621 ggcgttgtaa tataaaaatt tttttcagac ataatttttc ctttccaggc aaatgaaacc
    22681 tgtttttcta acacttcatt atatcacatt tttaatgaat ttcgataggg aaatccatac
    22741 aaaaacaaga tagacgagtg tccatcttgt tgatctcatt cataacgaag ggcttcaatt
    22801 ggatcaagtt tcgatgcctt gttggctggc aagactccaa aaatcatacc aacactagcc
    22861 gaaactgcaa gactaaatag ggcaactggg attgatactc caacttctat accttctatt
    22921 aaaccttgca gtaacaaacc tgctaaggca gttaaaccac ttgcaattgt caagccaatt
    22981 aagccaccta acaaggtcaa aatcatggat tcaatcaaaa actgaattaa aatattggca
    23041 cgtgttgcac ccaaagcctt acgaagacca atctcacgag tgcgctctgt caccgaaacc
    23101 agcatgatgt tcatgacacc agttcctcca acaaagagag aaatccctgc gatggaacta
    23161 ataatcgtcg tcataaaact aaacgattgt tgaatttctg caaatacaac ggactcatct
    23221 gccacctggt attctccctg ttgtaagcct gcaagctctg tcatttttcg tgccagttct
    23281 ggacccagag ttggggttaa actggtatca ttcactcgaa agacaatatt agctatttca
    23341 tctacattaa aattcgcagc aagggagata ttggtagtaa taggcaagcc accaaaccca
    23401 tatatttttg accttttagc ctccggacta gtataaaccc caatgacccg gtaactaaat
    23461 ccattgactt ctacaacctt gttaatagcc tcttgaggag attcaaataa actaatggac
    23521 aattcctcat ctagcaaaat gacacttgca aactctttga aatcttgctc tctcagacta
    23581 cgacctgcaa taatttcatt cttaacagcg tccatgtaag ttctgtttcc acctgtcaaa
    23641 ttagcattct caaccttttt atcttgatag gtcaagatgg cattcgttga attggttaca
    23701 tagtaactat ccactccctt cagtttagct gcctcttgga cccaggattc ttgcggtttt
    23761 ggcggttcaa caggaacttc ctcttccttt ccagaaaccg taaaagctga ttgtttctga
    23821 gtaaaagacc catctttact ttttttagga gagaaaaaga cgctaatatt tttctgagat
    23881 ttagtcatat ctttattgac ttgacgagat agggaatcac ccaaagccat aatcacaaca
    23941 actgatgaaa caccgataat aatcccaatc atagtaagca aagaacgcat cttgtgagcc
    24001 atgatagatg aaaaggcaaa tttcagattc tgcatcttag ttttcctcct ttcctaactg
    24061 agcactgtca gacgaaatga ccccatcccg aatgacaatc tgacgtttgg cataggcagc
    24121 aatctcaggc tcatgcgtta ccatgataat ggtttttcct tctttattca aatcaaccaa
    24181 taattgcata atttggttac ctgttttggt atccaaggct cctgtcggtt catccgctag
    24241 gataatagaa ggattgttta ccaaggcacg cgcaatggct acacgttgct tttgaccacc
    24301 agataattct gaaggtaaat ggtgactacg ttctgtcaat tcaaccttgt ctaaatattc
    24361 ctcagccaac ttgcgacgtt ttgaagacga aactcctgcg taaatcaagg gcaattctac
    24421 attttgcaga gcattgagct tcgatagaag aaagaactgc tgaaagacaa aaccgatttg
    24481 ttggttacgg accttagcta gttgtttttc accaagccca gccacttctt gaccttcaag
    24541 ataatattct ccactggttg gtgtatccaa catgccaatc gtattcatca gagtggactt
    24601 accagaccca gatggtccca tgatggctac aaattcaccc tcattcactt ctagattgat
    24661 atttttgaga acctgcagtt cttggtcacc attacggtaa cttctgaaga tattttttag
    24721 actaattagt tgcttcatca gccttcacct cttttccttc ttccaaggaa gatgttggat
    24781 tactgatgac cttagcaccg ttcgttaaac cagaagtgat ttcttgattt tctgcgtcag
    24841 catttcccaa tgaaacctca acttttttag ccttttgttg ttcatccaca atccagacat
    24901 aatttttact atcatccatt actagactgc taacaggaac aagaatagcc ttagttttgc
    24961 ttttaacctc aatgttgaca gaaaaacctt gtttcaaatc accaacctcg cctgtcacat
    25021 caatagtata agggtattta gaacctgtat tattcccggc tgctggacta gctgcttcac
    25081 cattgttttt aggatagtca gaaatatagc ttaatttccc agtccatttt ttatcaggat
    25141 acactttaga agtaaagctt acttcttgac ctacagaaag gttggctaga ttgtactcag
    25201 acaattctcc cttgacttgt aaattttcat tgctgacaat atgaaccata acttgactcg
    25261 cccctgttgg agatttagaa acattgctat tgacttcgac tacagttccc tctagggtac
    25321 tgagaacagt tgttgcatcc aattgacttt gagccttgct taattgcgct gcagcatctg
    25381 cacgcgcatc acgggcatca cccaattgag catcaataga agcaacagaa tttccagcca
    25441 ctggagttgg gctttgcacc gttgcatctt ctcctcctac tggcgctggt aactgtggag
    25501 cctgagctga agcggcttca tttcgtgctt gattgagttc attgatatga cgatctgcct
    25561 tagctactgc tcgactagct gaatcatagg ccgcctgcgc ttctgaacta ctgtacttga
    25621 ctaaagcctg cccttcgctg accttatcgc ccacagaaac aaggatttca tctaaatcac
    25681 ccttactagc atcaaaataa acatattgtt cattttttgc tgttactgtc cctgacaata
    25741 aaacagagga tgccacgctt ccttcattgg caacaacaag atgagtaggc tcatctttta
    25801 gagcagtctg agaaggttgt ctaaagagta aaatcccccc ggcacccaat acaactacac
    25861 tcgcagcacc gattgctgca tacagttgcc actttttagc tttaccattc tttttcttca
    25921 taatgaaact ccttttcttt tttacaatac tttgctatta taccaaattt ccctccagca
    25981 aacaatgcag ttcagatgga aataatggca gttcaggatt aaacaatcgt tcggaatttt
    26041 gcttttctat tgtactagat atataataca ctatcttgac ttatttggca agcttttttg
    26101 caattttttg tgcgtagcag atttttgcaa tcaaatcaaa aaaagaataa aatataacta
    26161 tcaaaaaaga cactctacta tttaaaagat tacaaaggag ttttcaatga gagaatatga
    26221 tatcattgct atcggtggag gtagcggagg aatcgctacc atgaaccgtg ctggtgaaca
    26281 tggagccaaa gcagccgtta ttgaggaaaa gaaattaggt ggaacctgtg tcaacgtcgg
    26341 ttgtgttcct aaaaaaatca tgtggtacgg ggcgcaaatc gctgagactt tccatcaatt
    26401 tggagaagac tacggcttta agactactga tcttaacttt aactttgcaa ccctacgtcg
    26461 caatcgtgaa gcctacattg atcgcgctcg ttcttcttat gatggtagtt ttaaacgcaa
    26521 cggtgtagac ttgattgaag gtcatgctga atttgtagat tctcatactg taagcgtaaa
    26581 tggtgaactg attcgtgcta aacatatcgt gattgctaca ggtgcccatc caagtattcc
    26641 taatattcct ggtgctgagc taggtggctc ttctgatgat gtatttgcct gggaagaact
    26701 tccagagtca gttgccattc taggcgctgg ttatatcgcc gttgaattag ctggcgtact
    26761 ccacactttt ggtgtcaaga cagatctctt tgttcgccgc gatcgtcctt tacgtggttt
    26821 tgattcctac atcgttgaag gtttggtcaa ggaaatggaa agaacaaact taccacttca
    26881 cactcacaaa gtccctgtca agttagaaaa aactactgac ggcattacca ttcatttcga
    26941 agatggtact agtcacacag ctagccaagt tatctgggct acaggccgcc gtccaaacgt
    27001 taagggcttg caacttgaaa aagctggagt gactctgaac gaacgtggct ttatccaagt
    27061 ggatgaatac caaaatactg ttgttgaggg aatctatgct ctaggtgatg taacgggcga
    27121 gaaagaactg actccagttg caatcaaggc cggacgtacc ctatctgaac gtctctttaa
    27181 cggaaaaact actgcaaaaa tggattactc aactattcca actgttgtct tttcacaccc
    27241 tgctatcgga actgttggtt tgacagaaga gcaagctatt aaagaatacg gtcaagacca
    27301 aatcaaggtt tacaaatcaa gctttgcatc tatgtactct gcttgcactc gcaaccgtca
    27361 agaatcccgt ttcaaactca taacagctgg ttcagaagaa aaagttgtcg gacttcatgg
    27421 aattggctac ggcgttgatg aaatgattca gggatttgcc gttgctatca aaatgggagc
    27481 aaccaaggct gactttgacg caactgtagc gattcaccca acttcatctg aagaatttgt
    27541 aaccatgcgt taatcgaaac aaaagaagct gatataaata tgtctgcttc ttttttaatg
    27601 attttcgaat caacaaaaaa ttatccaact tgactagtca cgaaaatcgg ctgttacagt
    27661 ttttgttacc ttgtttttaa aaaatggttg acatcgactc ctatatgagt ataatagtta
    27721 ctatacatta gaaaagaggt taactatttt gaaaaaagct cacgtttatg ctatccctgc
    27781 tattggggct gctctcattg ctgttttggc acaaatcagt cttccaattg gacctgttcc
    27841 cttcactctg caaaactttg caatcggctt gattgctact gtctttagac cgagagaggc
    27901 tgtactttct gctggactct atcttcttct aggtgctatc ggtcttcctg tctttgcagg
    27961 aggtggagct ggttttcagg ctttagttgg ccctactgca ggctatcttt ggttttatct
    28021 cgtttactct ggacttactt cctctctaac caacagcaag agtggtgttg ttaagatttt
    28081 tcttgcaaac ctcttgggtg atgcccttgt ctttgtcggc gggattctca gcttgtattt
    28141 cctagctgga atggcatttg aaaaagctct tgttgtgggg gttcttccct ttatcattcc
    28201 agaccttggc aaacttctgg ctattagttt tattagccgt cccctacttc aacgccttaa
    28261 aaatcagact tactttacta actaaaaaag gataccgagt tatcatgact caatatcctt
    28321 ttttttattt tgaaaactta tactcaatga aaatcaaaga gcaaactagg aagctagccg
    28381 caggctgctc aaaacactgt tttgaggttg tagatgaaac tgacgaagtc agtaaccata
    28441 catacggcaa ggcaaagctg atgcggtttg aagagatttt cgaagagtat tagttgcctt
    28501 taaaggcatc caccatcgtt tgaaattctt catttgagag agtaatccct ttgcccattt
    28561 tagtatggtc tggactccaa gcacgaatat caaactttgc aggggcacca ttaaagctca
    28621 cacggttaat ttccttggtc caaccttttt cgttttcaga aagagtcaac aagtgctctt
    28681 cgatttcaaa tgtaaattct gccattttct tctccttttt tagtttcatt agtttattcg
    28741 taaaatcttg tagattttag gaaaatttta tataatattg atataaaaga agggaggcca
    28801 atatgagaca taaattccag caagttctag ataaaataca tgatttttta aatggacatg
    28861 accaacctga ccagactgaa accaactccc ttacagccac tattgaagag gctatccaga
    28921 aacaaaccgc tgttcacctt atcttgtctg agacaagctt tacaggtgat atcatcaaat
    28981 atgatcagca aggccagcaa attatcgtga aaaatttttc caaaaatgtg agccggatta
    29041 tccgtataag cgatattcaa cgcctgcgat ttgtcccctc aactgtccaa acagcccaaa
    29101 aaaatagatt taagaaagag tgagatgtag ttgcttcatc ccactctttt tcttagcgaa
    29161 tttgttcaaa atgtaaatga actgcgatat gatctccata accacttctt tccaagtcac
    29221 gttgtaaacg ataggaaata tagtgttctg caatggtaat gtaacctgcg cccaataaac
    29281 gatgttcaac catagattga atcatactga tagtcgcacg ttccaccttg gcttcttgta
    29341 aatccaaaac taccttctta gtgacttgag caagattttg acgcaaatca tctgtcaaaa
    29401 cataaacagt ttgggctgcc ttcaagatgg cttggtaaat cttatctgga ttaaattcag
    29461 caatttcgcc attacgtttg attacttgca taggtttctc ctttattctt tgttttcttt
    29521 gatttctgcc agcatttttt cttcttctac tgtcagttga taatgttcaa gtaaatccgg
    29581 tctgcgctcg taggttttct ttaaactctc gtacaatcgc cactgacgaa tcttttcatg
    29641 atggccactc atcaatacat ctggcacgac catgcctcga taatcatagg gacgtgtgta
    29701 ctgaggatat tctaaaagac ctgaagaaaa actatcatct tggtggctag actccttgcc
    29761 aatcacttct ggaatcaggc gaactgtagc atcaatcatg gtcatagctg ccaattctcc
    29821 accagtgagg acatagtcgc ctagggaaat ctcatctgtt accaaggtct taatgcgctc
    29881 atcataaccc tcatagtgcc cacagataaa gattagctct tcctcttgag ccaaatcttc
    29941 agcataagcc tgatcaaact gctttccagc aggatcgagg agaataacgc gcggattttt
    30001 cttttcaata gcatcaaagg aatcgaaaat aggttgtgct ctgagcaaca tgccctgacc
    30061 gcctccgtag ggctcatcat ctacatgacg ggccttttca gcattttctc gaaaattatg
    30121 atactggata tccaagagcc ctttttctcg agcctttcca acgattgagt gctccagtgg
    30181 agaaaacatc tctggaaaga gggttaaaat atcaatcttc atcgtctaac ccttctaaga
    30241 tttccacatc gacccgttta tttggaatat caacattgag aaccactggt gggatataag
    30301 gtaaaagcaa atcacgtttg ccttttcgtt tgaccaccca gacatcatta gcacctggtt
    30361 gcaggatttc cttgatggtt ccaaccaagc tatcaccctc atagacttcc aaaccgataa
    30421 tctcgtgata gtaaaattca ccatcgtcta ggtcattcaa atcttcctca gcgaccttga
    30481 gactgtatcc cttgtacttt tcgatagtat tgatatggta catatctttg aatttaataa
    30541 tgtcaaagtt cttctgttta cggtggctag cgatggtcac tgtttggaca aactgatctt
    30601 tttcatcaaa caaagccagc tcagctcctt ttttaaaccg ttcttctgca aaatccgtca
    30661 cagacaagac tcgcatctca ccctgtaatc cctgcgtatt aacgattttc ccaacattaa
    30721 agtagttcat cttgtctcct gtaatctcct tttttccatc ttattctgac aattctcgaa
    30781 taatagccgc aattttttcc gattctgacc attgtaaata atggtgattc cctcctaaaa
    30841 tgagtttagt attggaagtc caatattctg attctctgta ctctttttct ctataagtct
    30901 gacaaaaaat aaatacaggg acatgagatt ctatcgacaa attctcaaaa tcttcctcag
    30961 taatctctcc agatatctga aattctggat cttgattttc caactctaag cctttttctt
    31021 gcattaattc ccagattttt ttattcgttt caggactaaa tgttgcttga gttaagttct
    31081 taaaataaag ttcaggacca cactcgtcaa tcagcctcat ctgctcttcc atttctggat
    31141 aaggattttc tgaaaaatca gcaaacatga cttttttagt tgtcggttca attgctacta
    31201 aagtctgaca cttaattggt ttctcgagta atttgcaagc taaaattcca ctccaactat
    31261 gtacacaaag tatatattca gaaatcccta attcttcaag tgcttcataa accacatctg
    31321 caagattatc tagatttttt ccagcttggt cataaatcgg actcctacct gtgttcggaa
    31381 aatcaattgt caaataacca attgtaggag gaggtttttc aagtataagt gaaaaatttt
    31441 cataacttgg tagcaaacct gctccattta gacaaactag cactttcttt tgcttttgat
    31501 aagcaacaga gagactacca atttctgtag atacttcaaa cctcttcata aagaaatcca
    31561 ctcattctat ataatgaatt attaaaaatc attatccttt attttatcac gttccaagga
    31621 ttttctcaag ttggaggaag gggacaacat ctctactttc ccttcaagaa tccttccaaa
    31681 ttatgtttat gttggtaatt aacggctgcg gttttgtctt tctcaaagac agtcttggta
    31741 aggtcaatat gattaatagc tacgattgcg acggtgtagt aaatgatatc agccagttct
    31801 ctggcaagtt cctcgttcga atcctatccc ttcttttcga ccagagcgcc tattcaaaac
    31861 ctcggctact tttccgactt cctccactaa cttcataaag agaccttcat cagtccgaga
    31921 ctgctgttaa tgttcgatta agtagtcttg gaattgccta aacgttcaat cttttatagt
    31981 atattgaaac tagaatagta cacctttact tctaaaacat tgttagaaat cgatttgact
    32041 gtcctgatcg atttgtcctg ttcttgtttc attttactat atcttctatt ccacacaaaa
    32101 aagcgaaaca tccgtcccgc ccttcttatt tttcgtcaat aacgattctt acttttttgt
    32161 attcagttgg gacagagtag acaatcgttc ttatcgcaga aatagtgcga cccttacgac
    32221 cgattacacg acccacatcg ctttgatcaa gattcaaatg atattccaaa aattctggtg
    32281 tatcctcaat cttgatagtt aaggcatctg gttgtgaaat taagggtttc acaatcgcaa
    32341 taatgagatt ttcaatcgta tccatctgtc aacctacttt aaacttattt tgaaaattta
    32401 gaatcgtgga attttttcaa tacgccttct tttgaaagga tgttacgtac tgtgtctgaa
    32461 ggttgagctc cattagccaa ccatgcaaga acgcggtctt ctttcaaagt tacttggttt
    32521 tcagcaacaa gtgggttgta agttccaact gtttcgatga aacgtccgtc acgtggtgaa
    32581 cgtgaatctg ctacgttgat acggtagaaa ggttttttct tagaacccat acgagtcaaa
    32641 cggattttaa ctgccatttt taaagtctca tttctttaat tttttatttc ggtgaaatag
    32701 ctgagctatt tagcacatgt tctattatag cagatttctg gcatgtgtca agaaaaaaac
    32761 ttgacagact ataaatttct taaagtattt agaaatttgt tagaatagga aataaatgtt
    32821 tgaaagatgc tatccgtgaa tactatttta gaaaaatttt ataaagaaca ccaagtcaaa
    32881 cccttcatat cgcctgaacg agagttggat acttggcttc taagtcccaa gcccgttccc
    32941 aagcgaaata tggacttatt agtagatgat tcactagcag gagatatcat tttactatgg
    33001 agaatccagt ttggtacttt taccactgaa acatgatttt aaaaactccc attcgaatat
    33061 gattcaaaat tcgcttagaa tcaatgtttt cccacttttt caaaaaataa attttcatct
    33121 aatttaaata actttcaatt aagtgtgaat tttgcttaaa atcattaaaa aacaccccat
    33181 ggtgtgaacc atgaagtgtt gtaaatgctg tgttgtagct agttcttagc gtttagagaa
    33241 ctggctagct ttacgagctt tcttaagatc tggtttccaa aatactttga gtacacttat
    33301 ggtattgatg cccctaaaca tctaaaaact ctggtagaaa aagggtatgc tcttgtcgaa
    33361 acttcttttg actcacttga ccatctaaat actaccatga aaaagaatat cctgaagaag
    33421 aagggagtta ctggactttc taaaatgaaa tcttgcgacc tggatcaagc ccttcatgag
    33481 catttttcag aagaagaatt agctggtcac tttcatgtcc ttctatggac ttttttacaa
    33541 tggcattgct atcacaccca atacctatct aagcgcctgg ttcgtaaact ttattgcagc
    33601 tcttcctcta aatttcctaa ttgttgaacc aattgcccgt tttatactaa gttcttttca
    33661 gaaaccattt actggggaag aagttgaaga ttttcaagat gatgatgaaa tcccaactat
    33721 tatctaagcc agttctgtaa actactaata tttgaaatcc acttcctttt agggtacaat
    33781 ggttataaat gaatttttga gaggatcaga atgaaaaaac tagcaaccct tcttttactg
    33841 tctactgtag ccctagctgg gtgtagcagc gtccaacgca gtctgcgtgg tgatgattat
    33901 gttgattcca gtcttgctgc tgaagaaagt tccaaagtag ctgcccaatc tgccaaggag
    33961 ttaaacgatg ctttaacaaa cgaaaacgcc aatttcccac aactatctaa ggaagttgct
    34021 gaagatgaag ccgaagtgat tctccacaca agccaaggtg atattcgcat taaactcttc
    34081 cctaaactcg ctcctctagc ggttgaaaat ttcctcactc acgccaaaga aggctactat
    34141 aacggtatta ccttccaccg tgtcatcgat ggctttatgg tccaaactgg agatccaaaa
    34201 ggggacggta caggtggtca gtccatctgg catgacaagg ataagactaa agacaaagga
    34261 actggtttca agaacgagat tactccttat ttgtataaca tccgcggtgc tcttgctatg
    34321 gctaatactg gtcaaccaaa caccaatggc agccagttct tcatcaacca aaactctaca
    34381 gatacctctt ctaaactccc tacaagcaag tatccacaga aaattatcga agcctacaaa
    34441 gaaggtggaa accctagtct agatggcaaa cacccagtct ttggtcaagt gattggcggt
    34501 atggatgttg tggataagat tgctaaagcc gaaaaagatg aaaaagacaa gccaactact
    34561 gctatcacaa tcgacagcat cgaagtggtg aaagactacg attttaaatc ttaaaaacca
    34621 aaaaaaatac agtatccaca ttcggtactg tatttctttt actctcattc ttaagttaaa
    34681 ttattaaaat cccatatttg gtctatccag ccttcataaa agtctggctc gtggcagacc
    34741 ataaggatag atcccctata ttctttgaga gcgcgtttga gctcatcctt tgcatccaca
    34801 tccaaatggt tggtcggctc gtccagcact aaaacgttgt tttcacgatt catcaagaga
    34861 cagaaacgaa ccttggcttg ctctccccct gataatactt gaatctggct ttcaatatgt
    34921 ttggttgtca aaccacaacg ggcaagggct gcacggactt ctgcttgatt aagggcagga
    34981 aaggcattcc agacagcttc aagaggagtt tggcgattac caccttctac ttcctgctca
    35041 aaataaccaa gttctaaata atctccacgc tccacttccc cagcgattgg cgagataatg
    35101 cccaagagac tcttcaagag agttgttttt ccaataccat tagcaccaat aatcgcaacc
    35161 ttttgattgc gttcgaaggt aagatttaaa ggcttggtaa gaggacggtc gtaaccaatt
    35221 tgcaagttct tggcttggaa gataaagcgc cctggtgtac gagctggttt gaaatcaaag
    35281 gatggttttg gtttctcact ttggagttcg ataatatcca tcttatccaa tttcttttga
    35341 cgagacatag ccatattacg agttgcaaca cgggctttat tacgagccac aaagtccttg
    35401 aggtctgcaa tctctttctg ctggcgttcg taggctgcct ctagctgaga tttcttcata
    35461 gcataaactt cttggaactg gtagtagtca ccagagtaac gcgtcagctg ttgattttcc
    35521 acatgataga caatattgat aacgtcattg aggaatggaa tatcgtgcga aatgagaaca
    35581 aaggcattct catagttttg gagatagcgc ttgagccaat caatatgctc agcatccaag
    35641 tagttggtcg gctcgtccaa cagcaaaata tcaggctttt caaggagaag ttttgccaaa
    35701 agcaccttgg ttctttgccc acctgacaaa gaagttacat ccgtatccat gccaaagtcc
    35761 ataataccaa gagcacgcgc tacttcgtca atcttagcat ccaaggtata gaaatcacga
    35821 ctctccagac ggtcttgaag ttctcctact tcttccatga gagcatcaac atccgcaccg
    35881 tcttcagtca ttttcatata gaggtcattg atacgagctt cagctttgaa aagctcatca
    35941 aaagccgtac ggagaacatc acgcaccgac tgtctttcag caaggacaga gtgctgatcc
    36001 aagtaaccag ccgtcacata tttggaccac tcaacctttc cttcatctgg cagcatttta
    36061 ccagtcacga tactcataaa ggttgatttt ccttcaccat tggcaccgac caggccgata
    36121 tgttctccct tgaggagacg gaaggacaca tcttcaaaaa ttgcacggtc accaaaaccg
    36181 tgactcagat ttttaacttc taaaatactc attttaattc cttatcttgt ttttatgtaa
    36241 tcgtttataa aggagccaag ccagatagcc acccaaagtg ttggtccaca aatcatcaat
    36301 ctcaaagacg cgattgaaat caaagaaaaa gtccaagatt aattgcgtac actcgattcc
    36361 cagactcaca agaaaactaa aaagaaggac cttttttgtt ttccgcaaat ttggaaatag
    36421 ataaaggagt tggaaaatca gaggaaaaaa caagaagaca ttgaggatat tttgtaaaaa
    36481 aatccaacat aattgtccaa tgtcactcac ttcgcccagt ttccagagag aattgaaagg
    36541 agtcaaaaga aaaaccaggc gtccaagatg ctgaatacct ggagttccca cttccacggt
    36601 agcttgagga gtaaagcaaa aatagacaat gcataggcta taaacagcca ctcccaatct
    36661 taagattaat tctctttttt tagtcatttt acgtatttac cgctgcgact gccttctggc
    36721 ggtcacgttt cattgtgtta aagcgcaatt gtccacaagc tgcgtcaata tctgtaccat
    36781 gctcttgacg aaccacacag ttgccccctt ttttcttaag cgtatcatag aaagccaaca
    36841 cgcgctcttt gggactacgg ctatattggt catgctcact aactgggtta taaggaatca
    36901 agtttacata agacaatttc ttgatgttct tgagcaattc agccaattcc aaggcttgtt
    36961 ctacaccgtc attgacttca ttaagcatga tatattcaaa ggttacacga cggtttgttg
    37021 tctcaatgta gtattcaata gcagcaaaga gtttttcaat cggaaaggca cggttaatct
    37081 tcatgatact tgaacgaagt tcattgttgg gtgcgtgaag agacacggca agattgacct
    37141 gaaccccttc atcagcaaag tcacgaattt tatgagccaa acctgaggtt gaaaccgtga
    37201 tgtgacgagc accgatagcc attcctttat catcattgat agtacgaaag aaattcaaga
    37261 cattgttgta attatcaaag ggctcaccga ttcccatgac aacgatatgg ttgacgcgtt
    37321 catcctgacc acgctcagca aagtatttct gaaccagcat gatttgcgct acgatttcac
    37381 cgttattgag gtcacgttgc ttcttaatca aaccagaggc acagaaggta caaccgatat
    37441 tacagccgac ctgagtggtc acacagacag ataaaccata gtgttgacgc atgagtacag
    37501 tctcaattaa cataccgtca ggcaattcaa agagatattt gactgtacca tcagcagact
    37561 cttgcacaat accttgtttc aagggattga ccacaaactg gtcattgagc ttagcaatca
    37621 aatccttgga aaggttggtc atttcttcaa atgactgcac acgtttacgg tagagccatt
    37681 cccagatttg atctgcacgg aatttctttt ctccctgctc caatacccat tcctgcatgg
    37741 tttgatgtgt caaactatga attgaaggtt tcatttcttc tccttattct ctactcactt
    37801 ctgacgaatg acaaaatgac gttgtccctt gtcctctttc tgagaatgct ggtctttctg
    37861 acgacgtcta ttcttcttat ctgcattcga ctttcgttta gtttgagtcg gtttctttcc
    37921 ttttctagaa ggtgtttctt cttccgtctt acgcattttc ttgtcaaatg atgctcgctt
    37981 aggggcttca ttttctaaga caaaataggc acaaccataa ctacaatact ctaaaaggta
    38041 gtcttgtaaa cgactgattt tttcaagttt ttcttctgtt cggtcatcct tgtaaaaacc
    38101 tcgtaggcga agctgttcgt tgctccagtc ccccacgata taatcaaact tggttaagac
    38161 ttctgaaaaa cgctgattaa aagtcgtcac atcaaaggca tccttgatat tttcaaccaa
    38221 ggaaaaagct atcccttccg tttcgacctt atccccgtgt aaatggaact ccggaccagg
    38281 aaacttgtta tagttgtata attcaggtgc aatttctttt cgcatagata tcctttttcc
    38341 acgattactt aatactttat tctaccataa tttctagcag ttagcacgtt tctcataaaa
    38401 atgaaaaaag tctaacgatt ttgtcagacc agaatcttat aacctaaaaa agagaagaac
    38461 aattcttccc tccaactatc attatttagc agctgcgtac aattcatcta ctttattcca
    38521 gttgattact gaaaagaaag ctttgatgta gtcaggacgc acgttgcggt atttcacgta
    38581 gtaagcatgt tcccaaacgt ccaagcccaa gattggtttt ttaccttctg agattggtgt
    38641 gtcttggttt gctgttgaag tcacttcaag tttcccttct ttgttgacaa ccaaccatgc
    38701 ccaacctgaa ccaaaacgag ttgttgctgc tgcagtgaag gctgcttgga attcttcaaa
    38761 tgaaccaaat gttgcatcga ttgctgctgc cagttctgct gaaggagctg ttttctcggg
    38821 agtcatcaat tcccagaaaa gagcgtggtt caagtgtccg ccaccattgt tgataagtgc
    38881 ttgacggata tcagctggga tagattctac atcagcaagc aaggcttcaa ggtcttcacc
    38941 gatttcaggg tgtttttcta aagctgcatt ggcattgttg acataagttt gatggtgttt
    39001 gtcatggtgc aaatgcattg tttccgcatc gatgtatggt tccaaagcgt cgtatgcata
    39061 tggaagttct ggtaagataa tagccatctg taatacctct ttttctttct atatgaaaat
    39121 gataacgcaa acattcactt ttttcaagtt ttttgcttat agataaggaa atcactgaca
    39181 tactttctaa taaaataagc aaatcagcaa gaaacccacg acaagataat cctgacgcgg
    39241 gttgtagttt caacaaatgt caattgacct gactagcaat ctgtaagagt gccttttcaa
    39301 aaaggaaacc tttttcataa agacctgtct taatctgata gtctgtctca atcaaatagg
    39361 aaatagcttg cttcagaaag ctcaaagaaa gtcctctcga atctcttaat gcaaacttga
    39421 tttgataagg atttgggtta cgtcccagat aactacctaa actacttgca atctgcgatt
    39481 ctgtttggcc agactccgcc aaaatcttca cctgagtaaa agtccgaaat tgtcccagca
    39541 tgactgcaat cagtttgatt tcatcttccc cttgcaaggt caagtctctc accaaatcgc
    39601 gcgcctgatc catcttttta gtcagaataa actgagttaa atcaaaaata ttgtcctgca
    39661 aggtcttggg aattgcgtta acaatatcct cttcctcgat aacagaatcc gccttatagg
    39721 actgtaaaaa gagaagattt ttctggattt cgctaaattg aaaccccgac ttgatgagga
    39781 gattttcaaa agaatgattg gtaaactgca gaccttgttt ctgactccac ttttggaaat
    39841 actggcgcaa ttcttgttct tttacttcta ctgcatcgaa gaccttggca tcacgcttaa
    39901 gtaatttgac taaccgtctt ttgctatcca gctttccttc tgcaaagatt atcaacttgg
    39961 ttgttggaga aggattgtca aggtattcct caaatgactt aagctcatca tctgtcaaaa
    40021 agcgtttctt agcagtcgtg atatccataa aataatctaa tatcacgatt ttctcatccg
    40081 caaagaaagg aaggctgacc aactccagtt ccacatcctt gtaaactact tctttcatat
    40141 caaagtaggc aaagttgagg tcagcagaat catacccaat ctgtttcaac atttgactct
    40201 tcatcacttc aaactgaccc tgatctgtcc ctgtaaatag gctcaggctc ggtaaatttg
    40261 ataaagtcaa cttctgactt tcttcaatgg ctagcatcgt ctctcctttc ttcagatttt
    40321 tcgatttaat ttagtcaata tagcgcaatt tcccacggaa atcttctaag ctctcgtagc
    40381 ctttttccac catgattgct ttcagttcat tggtaatgcg gtcaaaagca ctgacgcctt
    40441 ctttgtgaag ggtcgttccc acctgcacca tacttgctcc acagaggatg tgttcaaagg
    40501 catctcgacc agtcagaacg ccacctgttc cgataatttg gatttgagga tttaaacgtt
    40561 gataaaaggc gtgaacattg gctagagcag tcggtttgat gtattctcca ccaattccac
    40621 caaaaccatt cttaggccga ataacgacag attcgtcttc tatatagagg ccgtttccga
    40681 tagagttaac gcagttgaca aacttgagcg gatatttgtt gaaaatagct gccgcttggt
    40741 caaagtgaac aatatcaaaa taaggtggca atttaattcc aagaggtttg gtgaagtaag
    40801 caaacacttc tgccaaaatc cggtctgttg tctcaaaatc ataggcaatc tgaggtttac
    40861 ctggaacatt tggacaggaa agatttagct cagtcagacc acgaaaatca ctctcttgga
    40921 cttttttcaa aatagtatgg gtttcctctg gagacatgcc gaccagagat aagaagaaag
    40981 ttcggttcga ctctttttcc tgcaaatcca aaagataatc caaataatag tctaagccat
    41041 tatttggcaa gcccatagag ttgatggaac caagtggaac atcttggtag cgtggctcag
    41101 gattcccctg acggaagtcc aaggtcgctg tcttagtaac aaaggttcct gccgctgagt
    41161 ttttgacctc ttctaactcc tctatcgtca tacaagccac acctgctgca ttcatcaagc
    41221 aattgtcaaa ctcaaaacca gcaatttgtg ttttcgttga taccatgatt ctaatcctcc
    41281 agatttcttt tattgctaat attataccat aattttaatt ctaggtttac tagattctgt
    41341 ggttctatct atttattcgg aaaagaaagt ctagtacaga agtcatgcta tggaaacagc
    41401 tagtgtattg gaagtttgaa caaggcttgg gtaacagact tttgaatgaa gtccaagcaa
    41461 caaggagaaa tctcaaccat gagtccattc catgactctg ttgctctaga aaaccttgca
    41521 atagtcaagt gataacgaat aaaactttgg attgaaatat ctccatgatt atctattacg
    41581 aggagaaatc tcaaccttta tcatttttaa ataaatatag caaatatagt aaaatgaaac
    41641 aagaacagga gaaatcgatc aggacagtca aattgattgc taacaatgtt ttagaagttg
    41701 aggtgtacta ttctagtctc aatctactat acatgtaaat ctctactttc gcaaaatgga
    41761 ttttcagaac agtatcccag tatagttaaa aactataaaa aagagaggac gttcccctct
    41821 taaaatctta ttttactgct tctttcaaag catctacctt atccaaacgt tcccatggaa
    41881 gatcaatatc tgtacgtccc atgtgaccgt aagccgatgt ttgacggtaa attggacgct
    41941 tgaggtccag catttggata atccctgcag ggcgaaggtc aaagatttga cgagccgctt
    42001 tttcaagttg actttcagct actgttcctg taccgaaagt atcgatacga acagaaacag
    42061 gttgcgcaac accgatagca taggccaact gcacttctgc cttcttagca aggcctgctg
    42121 caacgatatt cttggcaata tagcgagccg cataagaggc tgaacgatcc accttagtcg
    42181 catctttacc agagaaggca ccaccaccat gacgagagta gccaccataa gtatctacaa
    42241 taatcttacg accagtcaaa cctgagtccc cttgaggacc accgattaca aaacgacctg
    42301 tcggattgat aaagaatttt gtcttatcat caagataaga agatggaata acttctttga
    42361 tgaccttgtc aatcacatct tgatggattt gttcattagt ggcctctgga tcatgctgag
    42421 tagaaataac gactgtatct acacgtaccg gacggtcatt ttcatcgtac tcaactgtaa
    42481 cttgtgattt tgcatctgga cggagatagc taatttctcc agacttacga agttctgcca
    42541 gacgacgaac caatttatga ctgagtgcaa ttggcaatgg cataagctct tctgtttcat
    42601 ctactgcaaa tccaaacatg agcccttggt cacctgctcc aatcaagtcc agtggatctt
    42661 gatcagcatt tccacgaacc tccaaggctt cgttaacacc ttgagcgatg tcaggagatt
    42721 gttccaccaa agatgggtgt actcccaccg tctcagcaga aaatccatat tctgtattgg
    42781 tataaccaat ctctgcaatg gtatcacgaa ccacacggtt aatatccaca taggcatttg
    42841 tagaaatttc accaaaaacg tggacagaac cagtatatac agctgtttca gcagcaacgt
    42901 gcgcctctgg atcctttgct aaaatagcat ccaaaatcgc atctgaaatt tggtctgcaa
    42961 tcttatccgg atgcccctca gatacagatt cagacgtgaa taatttacgt tctgacataa
    43021 aaatgtcccc ccttaaaaaa tattgttata gagttacaaa gtactgatag gaaaactctt
    43081 cgtaataaat acctaccatc ttatcgggac tatataactt tatcattata ccatttttaa
    43141 tcgcaatttg cagtctttaa tgtagtgttt cggtttagat agttcgttct cataaagcaa
    43201 aaagttggag ctcagtactc caacaatttc tattaaaatg tttgacgttt agctttacgc
    43261 tctgcacgac cgcgagcgcg attttcagca cgcttggttt tacggcgctt ttcatcaacc
    43321 gcccattgaa ttttcttctt ataacccggt ttgacttttt tctttttctt tttaaccaaa
    43381 ccaatcattt cgatatcaag tttatcttgt tttttctcac ggttggcacg acgatcacgg
    43441 tcataggtat cttgaaattc cccgtctttg accatcttag gactaaactt gattcccaat
    43501 ttctccaact cacggatatc cgagtcatca cttggctgat aaagggtaat agctgtacct
    43561 ggtaggccat ttcgtccagt acgaccaaca cggtgaacaa aaaaagataa gtcttgcgga
    43621 atggcatcat tgatgacatg gctgacacct tcaatgtcaa tcccacgcgc tgccaaatct
    43681 gttgcgacaa tatactcaaa atccagattt tgcacctgat tcatgattcg cttgcgttca
    43741 cgaggggcaa tatcgccatg gatttttgca accttcaagc cttgagcagt cagatatgaa
    43801 tgcaattcat cagcacgcgt tttagtgtta acaaaaatca ttgccaaata cggctgcatc
    43861 aactgagtca actggtaaat ttgagcattc ttatcatgtc ccttggtcga aatcaaccaa
    43921 ttatcaatgg tgtcagaaat aaccgttttg gtcttaattt tctccataac aggattggat
    43981 aagtattttt tcaagaatgg ttgcagtttt tgtgggatag tcgctgagaa gaccatgaat
    44041 tgcaagtctt ttggaagact gccagcaatc ttatcaacag tttccaagaa tcccatatcc
    44101 aaggtcatat ctgcttcatc aacaacaaat gtcttggctt tatgaatagc taaatcacca
    44161 gatttaacca agtcgtagat acggcctggt gttccaataa caatatgagg ctgattgctt
    44221 tccaatttct caatctggcg agccttatcc gtaccaccca cataattaac cacacgaact
    44281 tcgacatctg agtgagctga aatctgacgc gctacttggt aaatttgagt agccaactca
    44341 cgactcggtg cagtaatcac tgcttgtaca ctatcgctag cttcatctaa ttgctggaaa
    44401 atcggtaaca agaaagtatg agtcttacct gaacctgttt ttgattctcc tactaggtca
    44461 cgacctgcca aaacaatagg aatcaacttg tcttgcacct ctgttggagt tgtaaatttt
    44521 aactccttca aggcttctct aatatagttt ttaaattgaa atttcgtaaa tgacataaca
    44581 tcctcgattc tatctatctt atcaattata ccatatttta ttccattaca gtagtctcac
    44641 ttatttaggc tatttccagt agcttctcta gtaagaaaag gttggaattt tatagttcca
    44701 acctcttttc agttattatt tccagtttaa tatagcattt aagccatagt gatcactcac
    44761 ttgtggactc ttgttaccat caaatacgac atgtaaattt tccaccgcta actctttggt
    44821 agtaaagaca taatcgattc gaaggggttc agtgttccct ttccagccat caatttcagg
    44881 cggaacagta tagctaccac ttttctcttg agcaacttca aatgcgtctt gtaagcctaa
    44941 tagactagct aaaatagctt ggtaaccttc ctgacctgct gggttgttaa aatctccagc
    45001 tagtaaaagt ggcttgttca attttttcaa gacagcctca aatcgtgccc attcttcttg
    45061 gaaaccttta tcccaccaag agagatgaac actggcaact gctagctcct tgccatcgac
    45121 tacagtttca gctagggcaa cacggcgagt atgatagtct gttggatcat ccacatctga
    45181 aaccaaaatt tctctggctt caataggtgt tttagacaag atagccacac cttcgtggta
    45241 gcggtcatag ccgatatggt tataagccca ggtccagtag taatttttcc cttgctctaa
    45301 caacttttca accaagagtc taacataatg gtcttggtga ataggctcag ctgctggcaa
    45361 agcttgataa aggtcattaa cctccacctc tgacgaggtc atctcctgat tgatttcttg
    45421 gaaacaaatc aaatcatagt ccttttcaag aatatcttca agcaaaatct ggaatttttc
    45481 ctctgcttct ttctccatcc aactgtgagt attgagtgtt aaaaatttca ttcttttctc
    45541 ccaaaataag aggttggaat acagcttcca acctcaagta tattacaatt ctactttagc
    45601 aactgctgtt ttagctgcaa gagaacctgt tttttctaat ttaactgatt taattgcatc
    45661 accatttgtg aagacaacta ctgttgaagt ttcacgtcct gctgcacgga tagcatccaa
    45721 gtcagctgtg acaaggagat ctcctgctgc aactttttgt ccttcagcaa catgaactgt
    45781 aaatggttta ccttcaagac ttactgtgtc caaaccaatg tgaaccaata cttcaagacc
    45841 tgcttccgtc acaataccaa aagcatgttt tgttgggaag atgcttgaca cagtacctga
    45901 aactggagat acaatgtttc catttgcagg ttctactgca aatccatcac ccatcatttt
    45961 ttgagcaaat actggatcct ttacttgttc caaaccaaca acttgaccgt ctgctactga
    46021 ataaacttcc tcagtaagat ctttgaagtg aacagtgttt tgttgtgctt cagtcatttg
    46081 gcttggaaga gtttcaggaa tgatttcacc tgaatcaagg atatcttgga tatcagattt
    46141 caaaatgtca gcttttggac cgtagatagc ttgaacccct tgtcctttca tcacaagacc
    46201 catagctcct tctgctttcc attgctctgc atttcctact ttatctgcat ctttaacagt
    46261 tacacgaaga cgagtcatac atgcatcaac atcaacgatg tttacacgtc caccaagaag
    46321 gttgataatg tttacagctt gagagcctgc tgcaactttc acttcgctgc tggtttcttc
    46381 tgaaccttca gcagtttcgt agtttccgtt gcgccctgga gttgcgtaat tgaatttttg
    46441 aatcatgaag tttgcgataa agtacatgat tacagcaaag agaacagtta cccaaacgaa
    46501 gttaacgata tccataccaa taccagcact gattgcaata ggtgtacgag tcaagaactc
    46561 gattgaaccg aatgagtgca tacgtaggtt tacgacgtca gccatagcga aggcagcacc
    46621 ttgaacaagt gagtaaacaa gatacatagg tgttgcgatg aacatgaaca tgtattcgat
    46681 tggttcagta acccctgtca agaatgttgc aagagctgtt gcaatcatca tacctttgta
    46741 tttatgtttc ttgtcagcat caacattacg gtagatagca acaatcacac ccatcaagat
    46801 accgaatgaa ccgatcattt gtccaacttt gaaacgagct ggatgtactg tatctaacaa
    46861 gtgttgatat tgactagcat cagtaccttt aaggtttaca aggtctgtta cccatgcaag
    46921 ccatagtggg tcttgaccga atacttgagt acctttagct gtaccagtta aaatgtcata
    46981 agtaccacca agagctgtgt agttcattgg gatagtcaac atgtggtgaa gaccaaatgg
    47041 caagagcaaa cgttccaaag taccatacaa gaatggtgca agaattggag cagtttcttg
    47101 tgagttggca atccagatac cgaagttatt gatacctgtt tgaactactg gccagaaagc
    47161 agcaagtaga attgcagcga ttgctgaacg aagaataact acaaatggta cgaaacgttt
    47221 cccgttgaag aatgaaagtg catcaggaag tttacggaag ttgtagtatt tgttgtaagc
    47281 agttgcccct acaaaacctg agataatccc tacgaatacc cccatgttca aggctggagc
    47341 ttcaagaaca ctgataaagt aatcagcaac tttgattgaa ccaccaaaga aagtagttac
    47401 catagcatct ggatttttca acatatcgcc tgatacacca aaaattgtac cagtgatacg
    47461 gttaatcaag atgaaggcaa gaccagcggc gaaagcacca ccagcacgtt ctttagccca
    47521 gcttcctcca atggctaggg caaacaaaat gtgaaggtta ccgataaccc cccaaccgat
    47581 ttgctcaaga attccacctg tgatgacaag tggtgtaaag gttgggttaa tcatcacgat
    47641 agacttaccg attgaaatca tcaaaccagc agccggcata accgcgataa ctaccatcaa
    47701 agccttaccg aatttttgcc aaaattcgaa agacaagaca tttttgaatg tatctttcat
    47761 cattcaaatc tcctttattt ttatttaggc aaacgtttta cgaaaacgtt tgcaccttct
    47821 atgatttaat tataccactt taattttttt tgtaaaggct tttataaaaa aattacactt
    47881 ttttctaaaa aatttct
//
LOCUS       DBERMR010000008        44690 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.008, whole genome shotgun sequence.
ACCESSION   DBERMR010000008 DBERMR010000000
VERSION     DBERMR010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 44690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44690
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.008"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(125..1759)
                     /locus_tag="ACL8A1_000531"
     CDS             complement(125..1759)
                     /locus_tag="ACL8A1_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201350.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNTGKKVNVSARV
                     LSSYIFSSLLMLFMFINIAFRFPFEEMPSFFSTMVAILLVLAFSTSFTAFYNVFYESK
                     DLASYRPYAFKESEIIMAKGLSVLLPALAGIVPILAYFLVLYIRLAPSLWLGLPLMLL
                     SLTLLFVSVTLVMVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSTEALVGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVITVLFTSAFLPYL
                     MMISLISKIRDSQIVPDIHPPYWLPLFFVGVFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIFAVQSFLPVLTLLGLSLYLGLSILSMIYLLVVWIIASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAINLGVGALIVLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(1740..2489)
                     /locus_tag="ACL8A1_000532"
     CDS             complement(1740..2489)
                     /locus_tag="ACL8A1_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1201351.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLTSSDLETSLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSAPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVEN
                     LRKDHPDQSLESIYLSLAGRKEEVSDASQGH"
     gene            2668..4122
                     /locus_tag="ACL8A1_000533"
     CDS             2668..4122
                     /locus_tag="ACL8A1_000533"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HGS1201352.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKTQGIQPMADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNDFHWHWYHFT
                     GTDYDAKRRKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYNW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(4212..6830)
                     /gene="alaS"
                     /locus_tag="ACL8A1_000534"
     CDS             complement(4212..6830)
                     /gene="alaS"
                     /locus_tag="ACL8A1_000534"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HGS1201353.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGQ
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNTSQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKIVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDAKEVNGRRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(6852..7337)
                     /locus_tag="ACL8A1_000535"
     CDS             complement(6852..7337)
                     /locus_tag="ACL8A1_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HGS1201354.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSQISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(7612..7851)
                     /locus_tag="ACL8A1_000536"
     CDS             complement(7612..7851)
                     /locus_tag="ACL8A1_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201355.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTAILFHLNDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(8112..9182)
                     /locus_tag="ACL8A1_000537"
     CDS             complement(8112..9182)
                     /locus_tag="ACL8A1_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS1201356.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYEAFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKLEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDPQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQDS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(9179..9952)
                     /locus_tag="ACL8A1_000538"
     CDS             complement(9179..9952)
                     /locus_tag="ACL8A1_000538"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1201357.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIIAGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(9949..10755)
                     /locus_tag="ACL8A1_000539"
     CDS             complement(9949..10755)
                     /locus_tag="ACL8A1_000539"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1201358.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(10736..11893)
                     /locus_tag="ACL8A1_000540"
     CDS             complement(10736..11893)
                     /locus_tag="ACL8A1_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1201359.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFEPEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(11952..12902)
                     /gene="murB"
                     /locus_tag="ACL8A1_000541"
     CDS             complement(11952..12902)
                     /gene="murB"
                     /locus_tag="ACL8A1_000541"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HGS1201360.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARVVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESLSVAKMYAGGFTPCKR"
     gene            complement(13014..13766)
                     /locus_tag="ACL8A1_000542"
     CDS             complement(13014..13766)
                     /locus_tag="ACL8A1_000542"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family glutamic-type intramembrane
                     protease"
                     /protein_id="HGS1201361.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLISLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            complement(13776..14507)
                     /gene="budA"
                     /locus_tag="ACL8A1_000543"
     CDS             complement(13776..14507)
                     /gene="budA"
                     /locus_tag="ACL8A1_000543"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HGS1201362.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            14649..15920
                     /locus_tag="ACL8A1_000544"
     CDS             14649..15920
                     /locus_tag="ACL8A1_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGS1201363.1"
                     /translation="MKKQSKYKEVVSYLKNGIESERFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(15940..16755)
                     /locus_tag="ACL8A1_000545"
     CDS             complement(15940..16755)
                     /locus_tag="ACL8A1_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS1201364.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVDAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKVQLTSLTNMGEAVNELQTGKIDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGTYQSYLEKAASLTEVEE"
     gene            complement(16912..17565)
                     /gene="phoU"
                     /locus_tag="ACL8A1_000546"
     CDS             complement(16912..17565)
                     /gene="phoU"
                     /locus_tag="ACL8A1_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HGS1201365.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(17577..18335)
                     /gene="pstB"
                     /locus_tag="ACL8A1_000547"
     CDS             complement(17577..18335)
                     /gene="pstB"
                     /locus_tag="ACL8A1_000547"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HGS1201366.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(18348..19151)
                     /gene="pstB"
                     /locus_tag="ACL8A1_000548"
     CDS             complement(18348..19151)
                     /gene="pstB"
                     /locus_tag="ACL8A1_000548"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HGS1201367.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(19162..20046)
                     /gene="pstA"
                     /locus_tag="ACL8A1_000549"
     CDS             complement(19162..20046)
                     /gene="pstA"
                     /locus_tag="ACL8A1_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HGS1201368.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(20036..20953)
                     /gene="pstC"
                     /locus_tag="ACL8A1_000550"
     CDS             complement(20036..20953)
                     /gene="pstC"
                     /locus_tag="ACL8A1_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HGS1201369.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(20953..21831)
                     /gene="pstS"
                     /locus_tag="ACL8A1_000551"
     CDS             complement(20953..21831)
                     /gene="pstS"
                     /locus_tag="ACL8A1_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HGS1201370.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(22405..23709)
                     /locus_tag="ACL8A1_000552"
     CDS             complement(22405..23709)
                     /locus_tag="ACL8A1_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HGS1201371.1"
                     /translation="MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQTFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGET
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(23710..24483)
                     /locus_tag="ACL8A1_000553"
     CDS             complement(23710..24483)
                     /locus_tag="ACL8A1_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HGS1201372.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSSTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRSVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(24473..24751)
                     /locus_tag="ACL8A1_000554"
     CDS             complement(24473..24751)
                     /locus_tag="ACL8A1_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HGS1201373.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(24751..25152)
                     /locus_tag="ACL8A1_000555"
     CDS             complement(24751..25152)
                     /locus_tag="ACL8A1_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HGS1201374.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(25362..25979)
                     /locus_tag="ACL8A1_000556"
     CDS             complement(25362..25979)
                     /locus_tag="ACL8A1_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HGS1201375.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLTLVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
     gene            complement(25969..26742)
                     /locus_tag="ACL8A1_000557"
     CDS             complement(25969..26742)
                     /locus_tag="ACL8A1_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HGS1201376.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKENPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(26753..27490)
                     /locus_tag="ACL8A1_000558"
     CDS             complement(26753..27490)
                     /locus_tag="ACL8A1_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HGS1201377.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIAEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKV"
     gene            complement(27495..28625)
                     /gene="hemW"
                     /locus_tag="ACL8A1_000559"
     CDS             complement(27495..28625)
                     /gene="hemW"
                     /locus_tag="ACL8A1_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HGS1201378.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(28709..29098)
                     /locus_tag="ACL8A1_000560"
     CDS             complement(28709..29098)
                     /locus_tag="ACL8A1_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HGS1201379.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIHITEE"
     gene            complement(29071..29497)
                     /locus_tag="ACL8A1_000561"
                     /pseudo
     CDS             complement(29071..29497)
                     /locus_tag="ACL8A1_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895042.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
     gene            complement(29498..30286)
                     /gene="lgt"
                     /locus_tag="ACL8A1_000562"
     CDS             complement(29498..30286)
                     /gene="lgt"
                     /locus_tag="ACL8A1_000562"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HGS1201380.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(30279..31214)
                     /gene="hprK"
                     /locus_tag="ACL8A1_000563"
     CDS             complement(30279..31214)
                     /gene="hprK"
                     /locus_tag="ACL8A1_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HGS1201381.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINIADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(31344..31520)
                     /gene="rpsU"
                     /locus_tag="ACL8A1_000564"
     CDS             complement(31344..31520)
                     /gene="rpsU"
                     /locus_tag="ACL8A1_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HGS1201382.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(31600..32109)
                     /locus_tag="ACL8A1_000565"
     CDS             complement(31600..32109)
                     /locus_tag="ACL8A1_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201383.1"
                     /translation="MNKEEFLKIKEAYKSARTEERKSIIGFITKKKDKEGNFLFTKSK
                     DKPYTTRNQYSGGGGNKKYTSGSRLSRPYDLSNHMWIDLSYKGNDILISLQSFDIDPN
                     SKELHVLYDRIGILFEQSKKIPIFKDCYTITKVSDAFLKMETTNWELPLSKADMEEMV
                     NYIINHYEE"
     gene            complement(32187..32894)
                     /locus_tag="ACL8A1_000566"
     CDS             complement(32187..32894)
                     /locus_tag="ACL8A1_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HGS1201384.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FTKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            33045..34073
                     /gene="queA"
                     /locus_tag="ACL8A1_000567"
     CDS             33045..34073
                     /gene="queA"
                     /locus_tag="ACL8A1_000567"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HGS1201385.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRIIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDAMFIY"
     gene            complement(34734..35123)
                     /locus_tag="ACL8A1_000568"
     CDS             complement(34734..35123)
                     /locus_tag="ACL8A1_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HGS1201386.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTGWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNTSGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(35177..35884)
                     /locus_tag="ACL8A1_000569"
     CDS             complement(35177..35884)
                     /locus_tag="ACL8A1_000569"
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HGS1201387.1"
                     /translation="MFRRNLNTSFVHAVDGIQYVRDDTRDKEEGIEYDDADNGDIIVK
                     VATKPKVVIKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTRTRTYDVNPETGHVTE
                     QVTVDRKEATDTVIKVPAKSKVEEVLVPFATKYEADNDLSAGQEQEITLGKNGKTVTT
                     ITYDVDGKSGQVTESTLSQKEDSQTRVVKKGTKPQVLVQEIPIETEYLDGSTLDKSQE
                     VEEVGEIGKLLLLQSIL"
     gene            complement(36585..37136)
                     /locus_tag="ACL8A1_000570"
     CDS             complement(36585..37136)
                     /locus_tag="ACL8A1_000570"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1201388.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDVEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGHGMMTEALKAVLDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            complement(37534..38358)
                     /gene="nadE"
                     /locus_tag="ACL8A1_000571"
     CDS             complement(37534..38358)
                     /gene="nadE"
                     /locus_tag="ACL8A1_000571"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HGS1201389.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(38355..39815)
                     /locus_tag="ACL8A1_000572"
     CDS             complement(38355..39815)
                     /locus_tag="ACL8A1_000572"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HGS1201390.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(39931..40419)
                     /locus_tag="ACL8A1_000573"
     CDS             complement(39931..40419)
                     /locus_tag="ACL8A1_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6773 family protein"
                     /protein_id="HGS1201391.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYIIYLPWIFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(40409..40618)
                     /locus_tag="ACL8A1_000574"
     CDS             complement(40409..40618)
                     /locus_tag="ACL8A1_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS1201392.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            complement(41003..41731)
                     /locus_tag="ACL8A1_000575"
     CDS             complement(41003..41731)
                     /locus_tag="ACL8A1_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1201393.1"
                     /translation="MKLNKLNFLKENIRNLYSSGVIYLSLLISFIPPILLTFFILKTQ
                     ETSLGIKHISNFYAMLGMLMAVIHANRVISRDFSHNTVSLFYNQQKNRMIYVLSNFLY
                     AISVSIIYALNGIVLLVIVSKLGIPGDLGLDFIVAIVVNTILLVLFYFLLSYIFYLYK
                     LKSGLVFGILVALLLFIPNILNTMMMNTSNDLFIKAIELLPFYSLPVFVASNTMSISQ
                     YLVVITTIILLYFFTLKKSKKYSF"
     gene            complement(41747..42617)
                     /locus_tag="ACL8A1_000576"
                     /pseudo
     CDS             complement(41747..42617)
                     /locus_tag="ACL8A1_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(42973..44259)
                     /locus_tag="ACL8A1_000577"
     CDS             complement(42973..44259)
                     /locus_tag="ACL8A1_000577"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HGS1201394.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESSEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAAQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQSGDMVRALKEGLINLYKEDGTSVTVRV"
BASE COUNT        13429 a        10020 c         8232 g        13009 t
ORIGIN      
        1 atttcttatt caaaacattc ctccctatat ttttgattac caatcttaat catttacaac
       61 tacattctaa caaactataa aagcgtttgt cgaattgaat ttatcaagca agcaaccaac
      121 tatctcatct tttttctatt tctgccaata tgcgtgacag gtaataatga tagccaaaaa
      181 tagcaagagc aagcaagacg ataagagctc ctactcccaa gttgatggca aggatagggg
      241 agagagactg aaccaagaat atgctcccaa ttacaagggc catcaggatt gcactataaa
      301 taaacaataa aactatggcg actatgccat ttgaacgatt caccaggtcc gtaatgctac
      361 tccaattggt tgacagattt ttaacgtcct taaagtaatg gtggcaagaa aggatgacac
      421 tggcaatgat ccagactaca agaaggtaaa tcatcgaaag gatggacaag cctagatata
      481 gagaaagacc gagcaaagtc agaactggta aaaaggactg aacagcaaag ataatccaaa
      541 atttcacttt cacataacga gcaaagtcaa agggtaaact cttcagaaaa tcaacatttt
      601 ccctctccaa ggacaaggca attgaatgca ggctggtgat attgttattg acaactgcta
      661 taaacactcc tacaaaaaac aagggtaacc agtatggagg atgaatgtct ggaactatct
      721 gagaatctcg gattttggaa atcagactga tcatcatgag ataaggaagg aaagcacttg
      781 taaaaagcac tgtaatcaca ccagtcccct gtcccaagag ggtgaggtgg tagcgtaaaa
      841 ccatacggaa aaagcccttt ttagtagttg aaatcctctc cttgctgcga cgttcttttt
      901 tgaccttctc ctcactgtta agcaggatca cgtcataaaa acgaggaagg accttctttt
      961 tggtcagata aagcaggaag agagttagtc ctatccaagc gagcagaccc actaaggctt
     1021 ctgtcgaaaa aggctccact gctattttgt aaaagatatg aagaggataa aggagaaatg
     1081 gaatgtctct aactttgtca acaatacttc caaaagtcga ctgaagaaag aagataaata
     1141 ttaaaggtat gagaactcct atcccaatca tcacattcga aaaaatagac tgatactttc
     1201 tgaagaccct agtctgagcc aagaaatgta ctgccactac catcactaaa gtaacagaga
     1261 caaataataa ggtcaaggac agtagcatca aaggcaaacc caaccacaga gaaggtgcta
     1321 gcctaatgta gaggaccaga aaataagcta ggattggtac aattccagct agagctggca
     1381 agaggacaga cagacctttg gccattataa tctctgattc tttaaaggca tagggcctat
     1441 aggaggccag gtccttactc tcataaaaga cattgtaaaa tgcagtgaaa gaagttgaaa
     1501 aggccagcac cagtaaaata gcaaccatcg tactaaaaaa acttggcatt tcctcaaaag
     1561 gaaaacgaaa ggctatattt ataaacatga aaagcatcaa gagactggaa aaaatgtaag
     1621 aacttaggac tctagcggaa acatttactt tttttccagt atttttagct tgcttctttc
     1681 gtaggttagc cagattagct tcttgagatg aatagaggat attgatatca actaattttt
     1741 taatgacctt gagacgcatc tgaaacctcc tcttttctac cagcaaggct aaggtagata
     1801 ctttccaaag actggtctgg gtggtctttc ctcaagttct ctacactacc acaatagatc
     1861 aaatgcccct ttttcaaaat agcaatccga tcacagactt gctcggctac ctctaggaca
     1921 tgagttgaaa acaagaccgt cttgcctttt tgtgcatgtt ccttcatcat ttgcttcaaa
     1981 tcaaaggcgg cctggggatc caaaccagtc aagggttcgt ccaaaaccca aatatcagga
     2041 gcagacaaga gtgccccaat gacaaagact ttctgacgca ttccgtgaga aagagtttca
     2101 ataacctgat agcggttttc agcaaaatca aaaacgttca atagcctagc tagactagtc
     2161 tccaagtcag agctagtcag atcataggat gaagcgatca attcccaaaa ttcattggcc
     2221 gttaagcgta aaaataagtc aggcgagtct gctacgtagc caatctttcg tttgatcgcc
     2281 aagcgatttt ccgataactc ctgaccgtct accaaaatac gaccactgct gggtgaaatg
     2341 atactgacta gggattttat ggtggtcgat tttccagccc cattatgacc aatcaagccc
     2401 ataatctctc cattttcaat ctgcaaattg agattactca aggcctcttt atcaccatac
     2461 aacttactaa cattttgaaa ttcaatcatg ggacaactcc tatctttatc tgtattatac
     2521 atcgctatac tagcactttg atacaaaaaa atcaatactt tattttaagt agttaaaata
     2581 gtttccttct atcttacgca aacgtttgtg ctaatagatg ctttatgata gaataaagaa
     2641 caagattgac aagtaagagg aaacatcatg caaaatcaaa cactcatgca atactttgaa
     2701 tggtatctgc cccacgacgg tcaacactgg acgcgtctgg ctgaaaatgc tccacaccta
     2761 gctcatctgg ggatcagtca cgtctggatg ccaccagcct tcaaggcaac caacgaaaaa
     2821 gatgtcggct atggggtcta tgacttattt gacttaggag agttcaacca aaaagggact
     2881 gtccgcacca agtatggttt caaagaagac tatcttcaag ccattcaagc ccttaaaaca
     2941 cagggaattc aacctatggc cgatgtagtt ctcaaccaca aggctgctgc tgatcacagg
     3001 gaagcctttc aggttattga agtggatcct gtagaccgta cagttgaact tggagaaccc
     3061 ttcaccatca atggctggac tagttttacc ttcgatggtc gccaagatac ctacaatgac
     3121 ttccactggc actggtacca cttcacaggt acagactatg atgccaaacg ccgtaagtct
     3181 gggatttatc tgatccaagg ggacaacaag ggctgggcta acgaggaatt ggtcgataac
     3241 gaaaacggaa actacgacta cctcatgtat gccgacctag actttaaaca tcctgaagtc
     3301 atccaaaaca tctataactg ggctgattgg ttcatggaaa cgactggtgt agctggtttc
     3361 cgtttggatg ccgttaagca tattgactct ttctttatgc gcaacttcat ccgcgatatg
     3421 aaggaaaaat atggtgacga tttctatgtt tttggtgaat tttggaaccc agacaaggaa
     3481 gccaatctgg actatctcga aaaaacggaa gaacactttg accttgtcga tgttcgtctc
     3541 caccagaatc tctttgaagc cagtcaagct ggcgcaaact atgaccttcg tggcattttc
     3601 acagatagcc tggttgaact caagcctgac aaggctgtga cttttgtcga caaccacgat
     3661 acccaacgag gacaagccct tgagtctacc gttgaagaat ggttcaagcc agcagcctat
     3721 gccctcattt tgttacgcca agacggcctt ccatgtgtct tttacggaga ctactatggg
     3781 atttcaggcc agtatgctca agaagatttc aaagaaatcc ttgaccgcct cctagccatc
     3841 cgaaaagact tggcctatgg agaacaaaat gactactttg accatgctaa ctgtatcggt
     3901 tgggtacgtt caggtgctga aaatcaatcc ccaatcgcag tccttatctc aaatgaccaa
     3961 gaaaacagca agtcaatgtt tgtcggtcaa gaatggacta atcaaacctt tgtagattta
     4021 cttggtaacc accaaggtca agttacaatt gatgaggaag gttatggaca attccctgtc
     4081 tcagctagat ccgtaagtgt ctgggcagtc aataccatct aatagctcat aataaccaag
     4141 ctaggtccaa gcggatttgg cttttttgta ttcacaaaaa gacctaccca aatggataga
     4201 tctttacttg attacaattt acctgctact gcatccaaca attcttggat cttagcttgg
     4261 ttgcttcctc ctgccatggc catgtctggt ttaccaccac cacgtccatc gatgattggt
     4321 gctaattctt tgacaaggtt tcctgcatga aggtcttttg tcttgcttgc tacaaggaca
     4381 ttgactttgt caccgatagc ggcaactagg acaagaagat cagagtagtc tttttgtttc
     4441 cagttatctg caaaagtacg aagggcaccg gcatcggata cagacacttg actagcaatg
     4501 taacgacgac cgttgacttc cttagcatct ttgaagatat cgcctgcggc tgcagctgcg
     4561 gctttttctt tcaactcagc attttctttt tgaagttgac gaagttgttc ttgaagtcct
     4621 tctaccttgt gaggtacttc cttgacttga ggtgctttca aggttgctgc gacagcttta
     4681 agagcatcct cttgttcacg ataggcttca aaggcttcct taccagtcac tgccaagata
     4741 cggcgagttc ctgaaccgat tccttcttct ttgacaattt tgaagagacc aatctcagaa
     4801 gtgttgccaa catgagtacc accacaaagt tcaatagagt agtcaccgat agtcacgaca
     4861 cgaacttcct tgccgtattt ctcaccaaag agggccatag ctcccatttc tttagcagtg
     4921 tcaatatccg tttcaactgt cttcacttca agtgcttccc aaattttctc gttaacttgc
     4981 tgttcaatcg cacgaagttc ctcagcagtt actgcttgga agtgggtaaa gtcaaagcga
     5041 aggaattcaa cttcgttaag agatcctgcc tgtgttgcgt ggtttccaag gatattgtga
     5101 agggcagcgt gaagcaaatg agtcgcagtg tggtttttca tgacacggtg acggcgattg
     5161 ctatcaattg ccaaggtata ttcttggttc aaggcaagcg gtgcaaggac ttcaactgta
     5221 tgaagggctt gaccatttgg ggctttctga acattggtca cagtagccac aatcttacct
     5281 gactcatcca agatttgtcc gtagtcagct acctgtccac ccatttcagc ataaaatgac
     5341 gtttccgcaa agataagaga ggcagttcct tctgaaacag ctcctacttc tgcattgtca
     5401 gcaacgatag ctaccaattt agaagacaat tggctagtat tgtagttgaa gacactttct
     5461 acagtgatgt tttgaagagt ttcattttgc atacccattg agccaccctt gacagctgac
     5521 gcacgcgcgc gttcttgctg ttctttcatg gctgcttcaa aaccttcacg gtctacagtc
     5581 ataccagctt cttcagcgat ttcttcagtc aattcaactg ggaacccata agtatcatag
     5641 agtttaaaga catcttgacc agcaatgaca gattgacctt tttctttcaa gtctgccaca
     5701 atgccttggg caaagtgttg acctgagtga agggtacggg caaatgattc ttcttcgctc
     5761 ttaacgattt tctcaataaa gtcacgtttc tcaagcactt ctgggtagta gctttccatg
     5821 atttttccaa cagttggaac caatttgtaa aggaaaggct cgttgatacc caatttttga
     5881 ccatgcatag aagcacgacg gagaagacga cgaagaacat aaccacgacc ttcatttcct
     5941 ggaagggcac catcaccgat agcaaatgaa agagaacgaa tgtggtctgc gataaccttg
     6001 aagctcatgt tgtcgccatc ttggtcataa accttaccag acaatttctc gacttcacgg
     6061 ataatcggca tgaagaggtc cgtttcaaag ttggtcttag ccccttggat aacggccacc
     6121 aaacgctcca aaccagcgcc cgtatcaatg ttcttatgtg gcaattcctt gtattcgcta
     6181 cgaggaacag cagggtctgc gttaaattgt gacaaaacga tgttccagat ttcaatataa
     6241 cggtcgtttt caatatcttc tgcaagcagg cgaagaccga tattttctgg gtcaaaggct
     6301 tccccacggt caaagaagat ttctgtatct ggtccagaag gtcccgcacc gatttcccag
     6361 aagttgtcct caattggaat caagtgactt ggatccactc ccacttcaat ccagcggttg
     6421 taagaatctt tatcgtctgg atagtaggtc atgtaaagtt tttcagcagg gaaatcaaac
     6481 cattcagggc ttgtcaaaag ctcataagcc caagtgatag cttcgtcacg gaagtaatcc
     6541 ccgatagaga agttccccag catttcaaac atggtatggt gacgcgcagt cttccctacg
     6601 ttttcgatgt cgttggtacg gatagccttt tgggcattgg taatacgtgg attttcaggg
     6661 ataatggtcc cgtcaaagta tttcttaagg gttgctaccc cagagttgat ccacaaaaga
     6721 gttgggtcat ttacaggaac caaacttact gatggttcta ctgagtgacc tttggtcgcc
     6781 cagaaatcaa gccacatttg gcgtacttgt gcactagata gttgtttcat attgtctcct
     6841 tattcacttg tttaatgtga ttggctttcc agcatttcca catagtcaat cgcgacacag
     6901 agggaaatga ctaggtctgc ataagcgtct tcaagaaccg ttacggtata ggtagaggtc
     6961 agatggaaga gttccttctt aatttccgca atcaactgat cgcgatcatc cagcaatttg
     7021 aaattcaaat cccagatatt gccctcgata cgaagaccta gattatcaaa ctcatactta
     7081 tctcgccaga aggtcaactt cttacgaatg acaaaactcg agccatcccg aagctgaatc
     7141 tcaaaacgag gaagcaaggt caagatttct ttactgatct gactgacttg ttcaccagcc
     7201 gcatcataga tggtaaaagt tttgggaatc ttaaaaaatg atccctccac ctgataggca
     7261 atttctcccc tgtcatcctt gatagcgaag cgttcgcctc caagacgaaa cttttgtttg
     7321 acaagaaatg ttttcatcaa cacctccaaa aatcaaaaga caagctcata tcacgaaggg
     7381 cgaaaaaccg cggtaccacc ttcattcaat gaacttgtca ttctcttgtt cttatgcaat
     7441 tgtatgattg agtagcatga cttcctagct tagatggctc gcagcaccgc catttctctg
     7501 gactaagaca agtgaaaatc aattctcaac tttcttatta taacgttttt ttaagcttgc
     7561 gtcaactgga aatgatctcc gttgaattag accaattccc tacatctctg attacttttt
     7621 caggatatat tttttcttac tgccattttt ctttttatcc caaattttca tattactaaa
     7681 cacagctact agaatatttc caaatataaa ggtgcctatc acccaatata tggactcagt
     7741 tgttaggtat tgtcgatcca agccatcatt taaatggaat agtatagcag tttggttaac
     7801 aatcataaag gttggccaga aacttttttt gaaaaaagta gacattttca ttatttgttg
     7861 ccgctttcta taaggttaat actcaataaa aatcaaaaag caaactagga agctagcttc
     7921 aagctgtact tgagtacggc aaggcaacgc tgacgtggtt tgaagagtat aggcttagta
     7981 tactactagg caagcaaata agcaaataaa caaataaaca actagaatag aaaaagatag
     8041 ggctctaaaa actgacttct attccttaaa aacgaaccag cttgactgat tcgtcttctt
     8101 acgtttatct cctacttccg atacatttta aactgtagga agaggtcgct atatttccct
     8161 gtccatttat ggtcaaattt ctcataaact tctaggtgtt tcatggtttc aacatcggga
     8221 tagaaggcct tatcttcctt tgtttcctct gggagcaatt ccttcgctgg taggtttggt
     8281 gttgaatagc cgacatactc cgcattttgg agagcatttt caggtttcaa cataaagttg
     8341 ataaaggcat aggctgagtc ttgattttta actgttttgg gaatgaccat attgtcaaac
     8401 caaagattgc tggcctctgt cggtaccaca taacgtagat tttcattttt ttctaacatt
     8461 tggctggctt caccagagaa ggtcacgccg attgcaacat tattctgaat catgtagccc
     8521 ttcatctcgt ccgcaacgat agccttgata tttggagtca gtttgtagag cttatccact
     8581 gtctcttcca actgctgcgg atccttggag ttgaggctgt agccgaggga attgagtcct
     8641 agtcccagca cctcacgcgc cccatcaaaa agcatgatag aattcttata ctccagcttc
     8701 caaaggtcat cccaatgctc aggcgcttca tctaccatgg tttcgttgta gacaattcct
     8761 aaggttcccc agaagtaagg gatggagaat ttattacctg ggtcaaagga ctggttgaga
     8821 aactctggtc cgatattttc gattccttca atttttgaat aatcaagcgg aaccaagagg
     8881 tcttcgtcct tcatcttgtt aatcatgtat tcacttggaa tggcaatatc gtaggtcgtt
     8941 ccaccctgct ttatcttagt gtacatggct tcgttggagt caaaagcctc gtactgaact
     9001 tgaattcctg tttcttctgt aaactgagtc aagagttcag gatcgatata gtctccccag
     9061 ttatagataa ccaatttttg actatctcga ctattgattt tactatctaa atgagtcgca
     9121 attccccaca agacaaggat aatcgctgca attcctgcta aaaatgaata gatttttttc
     9181 atgcttgctc ctccttctca cgagagataa agtaataacc tacaactagg ataatactaa
     9241 agagaaagac tagagcagaa agggcattga tttctagcga aatccccttg cgagcacgag
     9301 agtaaatctc gactgatagg gttgaaaagc catttcctgt tacaaagaag gtcacggcaa
     9361 agtcatctaa cgaataggtg aaggccatga aataaccagc aatgatagac ggagtcaggt
     9421 aaggaagcat gatttcctta aacatctgaa attgactagc tcccaagtca taggccgcat
     9481 gaatcatgtc gccattcatt tccttgagtc gaggcaagac catcaagacc acgataggaa
     9541 tggagaaggc cacatgacta gatagaacgg tcaaaaagcc aagtgaaaac ttgagttggg
     9601 taaagagaat caagaagcta gcaccaatca taacgtcagg cgcaaccatg aggatattat
     9661 tgagtgatag aaaggcttct tggtatttct tacgagactg gtagatgtaa atggcaccaa
     9721 aagtcccgat aatggtcgct atcaaggctg ataggaaggc caagaaaaat gtctgagcca
     9781 aaatcagcat gagtctccca tctccaaaca tggtttcaaa gtgagtccag ctaaaacctg
     9841 taaagctatt catatcatca ccagcattaa aggcatagcc aatcaagtaa aagataggca
     9901 ggtagaggac cagaaagacc agtcccagat aaaggttggc aaattttttc atcgttctct
     9961 cctttcctta gtcacccaca tggtgatgaa catggtcagg atgagaatca caccgatagt
    10021 tgaacccata ccatagttgt cattggttag aaaattctgc tcaatagccg tccccaaggt
    10081 gataacgcgg ttcccaccaa tcaaacgggt cagcatgaag agactcaaac ttgggataaa
    10141 gaccgactga accccacttc gcacaccgtt catagataga gggaagatga catgacggaa
    10201 ggtctcccac ttagttgcac caaggtcgta actagcattg atgagattat tatccatatc
    10261 gtctaagaca ttgaaaatcg gcaaaatcat aaaggggagc tcgatgtagc ttgcgacaaa
    10321 gataaaggaa aaatcggtaa aaagcaactg ttgtgaacca attccgataa attccaagaa
    10381 ttggttaata gagccatttt gaccaaaaat cccgataaaa gcataggctt taaggagcaa
    10441 attgatccag gtaggaagga taatcagcat gagccagagt tgacggtgct tgagacgggt
    10501 caaaaagagg gctgttggat agctgataag caaggtcaca aaggtcacaa tccctgcata
    10561 aagcactgag ttgaaactca ttttgaggta ggtcaagttt tgtgacgcaa agtaagattt
    10621 ataattttct aaactaaact ggccttcaat gttgaaaaag gattgtccga aaatcaagac
    10681 caagggtgcc aatacaaaga gggcaatcca aagcatgtag ggcactacaa agagtttaga
    10741 gcttgttttc ttcatctctt tcctcctcga tcgcattgat cagacctgct tcttgctctt
    10801 cgatttccac gtattcttcg atacgagcat cgaactcttc ttcggtttca ttgagacgca
    10861 tgatgtggat gtcttctggt tcaaagtcca gaccgatttc ctcacccacg atggccttac
    10921 gggttgagtg gatcatccat tcatttccaa gttcgtcata ggcgataatt tcataatgca
    10981 ccccacggaa gagctgggta tcgaccttaa cttggagctt gccttcttca ggaagggtaa
    11041 tgcgcaagtc ctctggacga ataacgacct caacaggttc atttggcttc atcccaccat
    11101 caaccgcttc aaagcgtttg ccgttaaatt cgaccaagta gtcctcaatc atggtacctg
    11161 gcaagatgtt tgactccccg ataaaggtgg caacaaagtg gttgattggc tcatcgtaga
    11221 tgtccacagg ggttccagac tggacaatct cgccatcatt cataacgaaa atccagtcac
    11281 tcatggcaag agcttcttcc tgatcgtgag tgacaaagac aaaggtaatg cccaatcgtt
    11341 gttgtaattc acgcaattcg tactgcatgt ctgttctcaa tttcaagtcc agcgctgata
    11401 aaggctcgtc caacaagacc acacggggtt ggttgatgat agcacgggcg atggccacac
    11461 gctgacgttg tcctccagaa agtttgcgga tggaacgttt ttcataacct tccaactgaa
    11521 ccatcttgag aacttccgct acacgctgct caatttcttt cttatcaatt ttacgcaagc
    11581 gaagtggaaa ggcaacattt tcaaacacat tcatatgtgg gaacaaggca taggattgga
    11641 agacggtatg tacgtcgcgc ttattggttg gaatatcatt gatacgaaca ccgtctagca
    11701 tgatatctcc tgtcgtcgca tccagtaaac ctgcaataat gtttaggata gttgatttcc
    11761 ccgaaccaga tgcacctaga agggtgtaga atttcccttc ttccaactca aagttgatgt
    11821 ctttgagaac cttggtgttg ctgtcttcaa aaactttaga gacgtttttg aattcgataa
    11881 ttggtttttt caattggcat aaattccttc tttttcatag attaaccgat cggggctctg
    11941 tcaggtcccc actacctctt gcagggagta aaaccacctg catacatctt cgctaccgat
    12001 aggctttcac ccaagatccg gacttctctt tcaagcgtaa tacctgagtg ttccttgact
    12061 ttttcgataa ccgattggat caagtcctcg tagtctttgg ccgttccatc tgcgacattg
    12121 atcataaatc ctgcatgctt ttctgacact tctacgccac cgatacgata gcctttcaag
    12181 ccagcttctg aaattaactg acctgcaaaa tgcccgactg gacgcttaaa gaccgagcca
    12241 caagatgggt attccaaagg ttgcttgagt tcacgtaggt gcgtcaagcg gtccatttcc
    12301 tgcttgataa cctgatgggt tcctggagct agggcaaatt taactgacaa gacaactgca
    12361 ccagactcct gaatagctga atggcggtaa ccaaaagcca agtctttagc agacagggtt
    12421 tcgatttctc catccttggt caagacctta caagactgca agatgtgagc aatctcgcca
    12481 ccataggcac ccgcattcat aaagacagca ccgccaacgc ttcctggaat accacaagca
    12541 aactcaaagc cagttaaact atgacggagg gcaatgcgag ttgtttcaat caagttagcc
    12601 ccagcttctg cttcaatggt atagccatca acagaaacgt tattgagctt gtcacacaag
    12661 atgacaaatc cacgaatccc accatcacga acgatgatat tgcttgcatt gccaagaacc
    12721 atccaaggga tattttcttg gttggcaaat ttcacaacgc gagccaactc aaaacgattt
    12781 cgtggaaaga ccaaataatc agcctctcca cctacttttg tataactata gctatgcaag
    12841 ggttccttaa aacggatatc aattccttct aagatttcaa gcattttttc tcttacagac
    12901 atgtcactct tccttttaca aaattcattc cattatacca tttttagaga catttgacga
    12961 ccataaaaat accttgtttg gattttgcat aagaaaaaga ggttcccccc tttttatgat
    13021 tttttacaaa agatttcctt ggttccatag gcgaccagaa cgagctccag tgctagaatc
    13081 acttcaacca agactggatt tgtcaaccag cctacttgga aaagagatgg tgccagatca
    13141 aagaaggcat gcaagccata ggctgctagg agataaatcc atttcttctg gcgaacagct
    13201 tggtaaaccc aaactgtcaa aagtaattgg aaaccaagcg ccaagattcg ctcaaaacca
    13261 agcaaataaa tctgccagac cgaaagtgac tgaatggttt ttaacatatt ttcagacagt
    13321 aattgcataa cctgtggatt ctgagtttga actgccgaaa gaacgatgta aagattgagt
    13381 aaactaataa ggcctagaaa aatcaactcc aagccaccat gccctaggcc ataggccaag
    13441 gcatctgcct tttccaaact tctctttttc tccaaccatt tgaagaaaac aagacgagcc
    13501 gtttcctcaa aaaatgctgc catggctagg ccatagataa tatagataag cggatgatct
    13561 tgcaagaggg caatactacc gtctttttga ggatgcaaaa tcaagatatg caccagtttt
    13621 tccaaaatct gcgaagagac aaagaaagcg actgcaccca agcccaagac agctagatta
    13681 atctggtatt tctttttggc aaaccaaatg cttcctatca gaaaagctag caacagcaac
    13741 atggtaataa tgatatgaat ggtcattttc ttctcctatt ctgccttttc aatatctttt
    13801 ttcatctcgt caacattgaa cttagcaaac aagtattgac ggtcttggac tgggaaacgt
    13861 tggtccaact ggtcaactgc tcccacctcg ataatgcctt ccttgatgac aaaatccatg
    13921 acatgtccac cgaaggtcaa atcatctgat atgaagtgca gatggtagcc tgccacactg
    13981 accccatgga aaatctcagg cgtccagaaa ccaacaatag tccccgcaac attgtcacga
    14041 ctatattccg gttgatgggt tgcgacatca gcaaacttgg tatcgggtgt tgacttagga
    14101 atcatacgca catgcatatg tgaaaattcc ccccgaatct tgatagagcg gaaaagattt
    14161 tccccatcat aataagactc aattcgttct ttcaattcct tgtctgtcat ctcaaagcgc
    14221 tggcggaaaa tgacctctgc ctgatgcggt accactgcag cgtaaggaat aagggcatct
    14281 ggtgacactt ccacaatttc tggctggtct cctgaccctt tggcctgata agccttgcca
    14341 tccaagacaa tcaattcccc atcaatggaa tccaaggttc ccaaaccaag gtcaccatgt
    14401 tctagcaatt ctcctactgt catagtacca ccataaaggc cagccatcaa ggctccaaga
    14461 gtattgtatt gaaataattt cactggttcc tgcacgttct tatccattca ctttcttgtc
    14521 tgttattcta taaatcttac tcctaagtat accacatttg cccctagatg tgaacgagag
    14581 aaacgctcta gacattgcca agaaggaaaa aaaagggtac aatgtaacaa aatcaaggga
    14641 ggtctggaat gaagaaacaa agcaagtaca aagaggtcgt ttcctatctg aaaaatggta
    14701 tcgagtctga acgatttccg acgggtagtc gcctgccttc tatccgtcaa ctgagccttg
    14761 actttcactg cagcaaggac accattcaac gagccctgct ggaattacgg cacgaacaat
    14821 acctctatgc caagcctcag agtggctact atgtattaga acaagggcaa catcaagacc
    14881 tagaaatcga ggttaccgac gaacatgcca gtgcctatga cgatttccga ctctgtgtca
    14941 atgaaacctt gattggacga gaaaactacc tcttcaacta ctatgacaat caagaaggat
    15001 tagaagacct aagacagtcc attcacaaac tcctatttga gcaagctctc tactgcaagg
    15061 ctaaccaact agtactgact tctggaaccc aacaagcctt gtttatcctc tctcaaatat
    15121 cctttcctag acaagccaag gaaatcttgg tggaacagcc aacctaccat cggatgaatc
    15181 gcctcttgat tgcacagggg ctggactatc aaacgattga acgaggcatt gatgggattg
    15241 acttggagga gctggaaggc cacttcaaaa caggaaaaat taaatttttc tacaccattc
    15301 ctagatttca ctatcctctg ggacattcct attctgagca agacaaacga tctattctta
    15361 acttagctgc caagtatgat gtctatatcg tagaggacga ttatctgggt gatttggact
    15421 ccaagaaggg ccaaaccttc cactatcttg atacagagga gcatgtcatt tatatcaagt
    15481 ccttctcgac cagccttttt cctgcccttc gtattacagc actcattctt ccaaatgcta
    15541 tcaaagaagc atttgtggcc tacaaaaata tcctagacta cgacagcaac ctcattatgc
    15601 aaaaggccct gtcactctat attgacagtc aattgtttga aaaaaatcgt ttggctcgct
    15661 tgaccaatca tgaatcttac caaaaacaaa tcgaggaaag gataactaaa acaccttgtc
    15721 cccttcctca ttatccccta cacgatggtt tattgctaga cctgagacag tatcctaaaa
    15781 tcgccagtct caaacacagt caactgggct tggacttctt tgaagaggcc tatttaagca
    15841 cctgtcctta tcaatttgcc aaggtgtccc tagacaatct ggaaaatgtt ttaaactatt
    15901 taaaagcaga attggaatga catccaactc tgctttttct tattcttcaa cttcagttag
    15961 acttgctgct ttttcaagat agctttggta agttccttca tccttaagtt tttggatgac
    16021 cttgtctacc acttctttca aatcatcact attttttcta agagcaacgg cattggcgtc
    16081 gccgtccttc atcttcaagc tgacagttgc gacagctaag ccagcgtttt tagcagcata
    16141 actaagtgca acaggctcat ccatatgaac agcatctatt tttccagtct gcaattcatt
    16201 gactgcttca cccatattag ttagggaagt taattgaact tttggcaatt gttccttgac
    16261 cattgattct ggaacagtcc ctttttgggc tgcaatatta gcactttcta ggctagttaa
    16321 atccttgtat ttttccacat cagccttacg aaccaagaaa ctaatcttgt tttcatagta
    16381 tgggattgaa aaatcaaaga cttcttttct ctcgtcagta gcactaattc ctgcaactgc
    16441 taggtcagcc ttaccagttt gaagactggt caaaacattg tcaaaactca tgcttgagat
    16501 ttccaactta accccaagtt catcagcgat agcctgagcc atgtcgatgt ctgcaccgac
    16561 tacctggttc tttccatcaa ccaatgattg aaattcaaag ggtgcatagt caggactggt
    16621 tgccacaact aatttccctt tttgtttgat agcatccaca gcagactgag aaccattaga
    16681 acctgactga caagctacta agaagaaact tgcaagaaaa ttacataaaa caaatatcca
    16741 tttttttgat ttcataaata aacaacctct ctgttaattt tgtataatta taacgatatc
    16801 caagttactt gtcaagtgtt ttttaaattt ttatctcaaa aatatttttt cgttcaaaaa
    16861 aaggagccat cagttgattt caagctcctt tttatacaga attaaactat tttatagttc
    16921 gacaatctta cctgtttcaa agtagacaac ccattcacag atatttttag catagtcacc
    16981 gatacgctcc aagtaggaaa taacttggaa ataatcacga cccgtaacaa tggcttctgg
    17041 atttttctta atctcttcag tcgcaaggtc acggatagtt tcaaaatagt ggttaatttg
    17101 ctcatccatg gaggccaccc ggtatgcgtc gtcaacagaa ccattaagat aaagatcaag
    17161 tgctgcttca acaacgcttt taacttcacg tcccattttt ttaatttctt cctctacagc
    17221 tggaatgcgc tcttccccct tcatacggat ggttgcctgg gcaatggcta cagcgtgatc
    17281 ccccatacgc tccacatctg atacagcctt aaggacagtc aagactgtac gcaaatcttg
    17341 agagactggt tgttggagtg cgatcatttc aaatgatttc ttttccagtt tcacttcgta
    17401 ttcatttact tctgcatcat cttcgatgac ctcttttgcc aggtcacggt catgcgtgac
    17461 aaaagcacgt accgtacgat tgatttgtga gagcacttct tgtcccatag cgtagaactg
    17521 gttatgtaat ttctctaaat cttcttcaaa ttgagatcgt aacatctttc atctccttat
    17581 ccaaattttc ctgtaatata gtcttccgtt tccttgtgtt ggggatcaag gaacatctgc
    17641 ttggtatcat taaattcaat caaatctcca tctaggaaaa atcctgtctt atcagagata
    17701 cgtgaagctt gctgcatgga acgggttacc agaagcatgg tgtacttgtc ttttagacca
    17761 tacaaggttt cctcaatttt accagctgaa atcggatcca aagccgaagt tggctcatcc
    17821 aagaggatga ttttaggact agttgccaag acacgggcca cgcagacacg ctgctgttga
    17881 ccacctgaca atccaatagc tgaatcatat agacgatcct tgacctcatc ccagatagag
    17941 gcaccttgca aggctttttc tacggcttca tccagaacct gcttatcctt aattccattg
    18001 atacgaagcc cgtagacaac attctcatag atagtcatag ggaaaggatt aggttgttgg
    18061 aaaaccattc cgatttcctt acgtaattca accgtatctg tacgcggact gtagatgttg
    18121 tgaccattgt acaccacgga tccagttgtg gtcacctctg gattgagatc tcccatgcgg
    18181 ttgagagact tgaggagggt tgacttccct gatccagatg gaccaatcaa ggctgtaatt
    18241 tccttaggtt ggaaagatag ggaaacacta ttcaaagcct tctttttatt ataataaacg
    18301 gacaggtctg atacctgtaa aatcgcatct gtcatacggt ttcctttcta accaaagtga
    18361 ccagatacat agtcattggt ggactgtagc ttggcatttt ggaaaatagt tgcagtcttg
    18421 tcatactcaa tcaaatcacc caagtaaaag aagcctgtat agtcacttgc acgagcagcc
    18481 tgctgcatat tatgcgttac aatgatgatg gtaaagtttt tcttgagctc aaacatggtc
    18541 tcttctagtt gcatggtcgc aatcggatcc aaggctgagg ctggctcatc cattaagagg
    18601 atatctggct taacagagat ggcacgagcg atacagagac gttgttgctg accacctgat
    18661 aaggtcaagg ctgacttgtg gagatcgtct ttaacctgat cccagagggc agcctgacga
    18721 agggaggttt ctacgatttc atctaggact tgcttatcct taactccagc acgttcatgc
    18781 gcaaaggtaa tattacggta aattgactta gcaaatggat tggggcgttg aaaaaccatt
    18841 ccaatgtgtt tacgcatttc ataaacgttg atttctggac ggttgacatc aattccacga
    18901 tagagaatct gcccagttac tttagcaata tcaatagtat cattcatgcg attgagactg
    18961 cgtaagtagg tagatttccc cgatcccgac ggaccaatca aagctgtaat tttatttctt
    19021 tcaaattgca tatcaatccc cttaatggat tcatttttac catagtaaac atggacatcc
    19081 ttagtagaaa gggctacttt ttcttcagga aaggtaagga tatgcttctc atcccagtta
    19141 tatgttgaca tggcttctcc tttaggcagc ggttaatttc ttgtgtagat agcttccgaa
    19201 cttacgagct ccaaagttaa aaatcaggat aaagatcagg agcacagcgg cagaacctgc
    19261 tgatacaatg gttccatctg gaatagtgcc ttcactattg actttccaga tatggacagc
    19321 caaggtttct gcttgacgga agatagagat ggggctagtc acactgagga tattccagtt
    19381 agaccagtca agagctggcg ccgattgccc tgctgtatag atcagagctg cagcttcgcc
    19441 aaagatacga ccagatgcca agacgacacc cgttacaata cctggaagcg cttctggaat
    19501 aacaacatga accactgtct cccagcgaga aatcccaaga gccagaccag cctcacgttg
    19561 ggtatggtga acgtgtttca aactatcctc tacattacgc gtcatctgag gcaagttaaa
    19621 gactgtcaag gccaaggcac ctgaaatgat tgaaaatcca tactcaaact ggactacaaa
    19681 gatcaagtaa ccaaagagac ccaccaccac tgatggtaaa gaggacaaga tttcaataca
    19741 agtccgcaca aagttggtaa caggaccttt tttagcatat tcagccaagt aaatcccagc
    19801 tcccatagaa agaggtacag aaataatcaa ggtaatgacc aataggaaaa aggaattgta
    19861 aagctgaatg ccaatcccac cacctgcttg aaaagcagaa gaccttccag tcaagaaaga
    19921 ccaagagata tggggcaagc cccgaaccaa gatatagaga atcaaggaag ccaagattgt
    19981 cacaatgatg ctagcaatcg tatagaggac agctgttgca agtttatcta atttcttagc
    20041 gcgcataatt tttctttcct ctttctttcg taatcaattt aatcacactg ttaaaaacta
    20101 aactcatcaa gagcagtacc aaggccagtg accagagaac attattattt acagttccca
    20161 tgacagtgtt cccaattccc atagttaata tagaagttaa agttgcagct ggtgtggtca
    20221 aggaagttgg gataacagct gagtttccga caaccatctg gatagctaaa gcctcaccaa
    20281 aggcacgcgc catcccaaag accactgcag tgaaaatacc agaacgggcc gccttcaaga
    20341 tcacacgcca gatagtctgc cagcgagtgg ctcccatagc gaaactggct tcacgataat
    20401 aacgaggaac cgcacgcaag ctatccgttg tcataaaggt tacggtcggc aaaatcatga
    20461 caaagaggac ggaaatccct gacaaaatcc caaaaccagt cccaccaaag acactgcgaa
    20521 caaagggaac gacgacttgc aagccaataa atccgtacac tactgaagga atcccaacca
    20581 ggagttcaat agctggttgc aaaatcttcg ccccttttgg tgatacttcg gtcataaaaa
    20641 ctgctgcacc aatagcaaag ggtgttgcga taagggctga gagaatggta acgataaagg
    20701 aacccaaaat cataggaagg gcaccaaatt ctttactaga aggattccaa gttcctccca
    20761 aaagaaagtc aaagatattc acaccattga caaagaaggt cgacaagcct ttttgcgcta
    20821 cgaaaaccaa aatcatggcc acaaggatga ctatcaaaga aagacaggca aaggtcaaac
    20881 cttttcctaa tttctccaga cgagaattct ttgatggaag caacattttc ttagctaatt
    20941 cttcttgatt cattattgtc tcccttccaa cactgtcaca gttccggcag catctttttc
    21001 aaccttcatt tccttaatcg gaatatactt caatcctttg acaatccctt cttgggtctc
    21061 atccgagaga acaaaattga gaaattctgc agccaactca ttgggctgcc ccaatgtata
    21121 catatgctca taagaccaca agggccaatt attgctactt atattttctg gacttaagtc
    21181 atagccattc aacttcatgc ttttgaccga atcatctata taggtaagag ataaataaga
    21241 gatagctcct ggactttttg atacgattga ttttaccgct ccatttgaat cctgctcctg
    21301 actttgcatg gcagactgac cttccataat gacagtatca aaggtagcac gagagccaga
    21361 gccggctgcc cgattgataa cagagatggg taagtcctta ccaccaacct ctttccaatt
    21421 ggttacctca cctatgaaga tttgacgaag ttgctctgtc gttaggttat caacatcaac
    21481 ctccttattg acaatcagag ccaagccagc taccgcgacc ttgtggtcaa caagagcaga
    21541 agcatcaatt ccgtcttttt cctcagcaaa tacatctgag tttcctatat caactgcccc
    21601 agactgaacc tgggacaagc ctgtaccaga acctccccct tggacattga ccgtttttcc
    21661 aacatgaatc gtgccaaatt catctgccgc tacttcaacc aagggttgca aggcagttga
    21721 gccaacagcc gttatggatt ctccacgatc aatccagcta gcacagccta ctaaacaagc
    21781 cgtcagccaa aaagcgataa gagacagagc aagctttttt ctttttttca ctgtttttct
    21841 cctcgaaaat aattatgaat actgtgaatt ttttaagtag ttctttatga gttgacgcat
    21901 gaattcttac caaatttctg cgcaattgat tatttatata atataggcta tattactctt
    21961 tcctaacctc cttttttcat atgtggataa aatctcttgt ctatcccttc ccccattgtc
    22021 acccattata atcatttcgt gtctcttttt ccccttttta atgcaaggga aattactctc
    22081 ctcagatgat aatccaaaag ctagaaaggt atctcaaacc tctctactct cccagactag
    22141 tttacaacta aaaggaaaag attctatttt atgagaaatc tagtttacaa gcggtaagaa
    22201 cgctaataac taaacttctt gtactctttg aaaatctctt caaaccagtg ttttgagcta
    22261 tctatggcta gcttcctagt ttgctctttg attttcatcg agtagtaaaa ctacatgtga
    22321 tggcaatcaa gatatcaaga atcatcctac taaaaaaatc catactttca ctataacata
    22381 gaataagata tttgactagc attttcattt gaatctgagg ccttttggaa aataattttt
    22441 caaaacattt ccagtaacct ttgcaaagcc caagccattg cctttaacca aaacttggta
    22501 ccaaccattt ggcagacttt ctgccagctg aacggtttct ccagccgcat acttgacaaa
    22561 cgcttcttgg ccaatttcaa ccgactgttc gacctgactc ggtttcaagg ctaaaccaag
    22621 agcgaaactg ggctcaaagc gtttcttctt aaaagtgccc agatgcagtc cattgcgagc
    22681 aatcttgagc ttccctaaat ctggcaaaag ttctggcaag agataaagct ggtctccaaa
    22741 agtctgcaag atacctggta gattgacctt caaatggttt tgggcaaatt cctgccacaa
    22801 ggcaacttgt tcacggctga ggttactctt acttgcctta aatttaggag ctggattgtt
    22861 acccttaaac tgtagatggg caacaaactg accctctccc ttaaactgat gagggtacat
    22921 ccgagccgtt tctggcaggt caataccagc taccattcca ttgatatgct ctactggcaa
    22981 caagtcaaaa tcatactctt ccagcaacca attgacaatc tcttcgtttt cctcgggtgc
    23041 ccaggtacag gtcgaataaa ccagacgacc accttcagct aacatggtca ctgcatcctc
    23101 cagaatttct ctttgcaagc tagcacattg actcggataa tctaagctcc aatagtccat
    23161 agcatcaggt tgcttacgaa acattccttc accagagcaa ggggcatcaa gaacgattaa
    23221 gtcaaaatag cctttaaaga ccttgaccaa gcggtcggca gattcattgg tcaccacgac
    23281 atttgtcgct ccaaaacgct ccatgttttc aaccaaaatc ttagcccgtt tgcttgaaat
    23341 ttcattggaa acaagtagcc cctcacctgc tagataggct gccagttgag ttgatttgcc
    23401 ccccggtgca gcagccaagt ccaagacctt cataccagga ctgggttggg ctacttgagc
    23461 caccatttga gcagcaggtt cttgcgaata aactaaacct gtagcatgct caggcgattt
    23521 ccctgaaacc ttcccatagt ggccccaagg ggtttgagta atggcatcag aaaaggaaag
    23581 ttgctcttct tttaagggat tgacccgaaa ggccgaaacc gcttcctcct caaaagaggc
    23641 aagaaaatct cttgcctcat ctcctagtat ctctttatat ttttcaacaa atccttctgg
    23701 aaattgcatt taagttcttt tcctttcgta aatataggac tgaatttcct cctgcatctc
    23761 aagaggcacc atcatgaccg gctgtctggt ttgaaaatca ggagcttcac caaaaagggt
    23821 cacaacccga tagcccagac tttcccctaa aatactagct gcggcataat cccatggttg
    23881 cagataagtg agataggtca acaaacgccc tgacaaaatc ttggcaaaac taatggccgc
    23941 acttccatag acacgaacac caagaaccga tcggctcaaa tcagccagcc cccattcatt
    24001 ggtttccagc ataccactat tccctgcaat gagaaaatct ccaagtggtt tagttttaaa
    24061 aggagctagg ggcctatcat ttagacaaac tggaaattcc ccaccaccgt ggtaacaatc
    24121 ccctttgacc acatcataaa tcagaccaaa ctgtccctga ccattttcaa aataagccat
    24181 cataacagca aaatcttcct gctgggctac aaaattattg gtaccatcaa tgggatcaat
    24241 gacccaaacc ttgccctctt gaaccgaggc tcgcagacaa ccttcttcag cacaaatctt
    24301 atcctcagga taacgggaca aaatctcacc aaccaagagt tcctgaactt ctttgtccag
    24361 tctggtcacc aaatctgttg aagaggactt ggtttcaaca cgcaagtctt cctgcatatg
    24421 gtcaagaatg tactggcctg ctttcttaac aagctcttta gcaaattcaa atttactttc
    24481 caagagaaat ctttccttcc cctttttctt tggcagcctg aactgctcgg taaagtgaat
    24541 agccactaac cgtttcaaat tctcgtccca aacgtttctc ttcgctcttg cttggcacga
    24601 ccgctttgaa tcccttatag gagtccagta actttttagc ctctactttg ccttcatagg
    24661 cagcttcaac atcattaaaa aaagaaagca ctgaagcaag ttcttcagtg ctccacgaca
    24721 aatctagtgg gtaactatac tgtttgttca ttaactaata ccagctctca ttcttgcttc
    24781 ttttagttct tgcttacgat aactacgagg gagaaaagca cgaatctcat cttcattaaa
    24841 accgatttgc atacgcttgg catcaataat aattggacga cgcaaaagac taggatactg
    24901 ctcaatcaaa tgaagcaatt ccgataccga aatactctct acatcaatat tcaatttttg
    24961 aaaaattttt gaacgagttg aaatgatgtc atcagtacca ttttcggtca aggaaaggat
    25021 gtgttgcaat tcttttcttg ttaaaggact ggtcataata ttgtgttcca caaagggaac
    25081 cttatgtttt tctaaccagg ccttagcctt acgacatgat gtacagctcg gtgatagaaa
    25141 tagtgtaatc atgcttttct cttcttatct atactttgct acttctatta tacaaaaaaa
    25201 taaggcgctt gactagggat ttttagaaaa aaagcctatt ttttcaagaa aaataggctg
    25261 tttgcgaacg attgacacaa ttggatttgg ttaattcact cttaacgatg gttttaaacg
    25321 atatatattt ttatatatgc aaattaaaaa catctttcat ttcacttcct acgacttttc
    25381 agacacagat acccaaagaa gctttcatag agggcaaaaa agaggaggaa ggcatgaaga
    25441 aagaaggtct ctggcaaaat cataataaca ggatccttgg ctggatcaaa aagccaggta
    25501 tcatctccca caaagagaat ttgatggaaa agagtaaaga attggtcaaa accaatcaaa
    25561 actcccccaa gtccaatcat cacaggtaag actactagag tcaggagact ttttcgataa
    25621 agagacaaaa agtccttttt cacaatccta ttgacaaaga catagaaact tggcagtgtc
    25681 actagagcta ctagctgaac caaatgaaag agattcttga ccactgcgaa atggtgcaga
    25741 ccagctgctg acgaacgaaa atcaggcatc tgtaagacct gactaaaagg attggtcaga
    25801 taattcatca agatatgaaa attgtattga atggtttctg gttttagata gactcgattc
    25861 gttaagttta gccactgaat ctccatagga tagaaaatcc aagccagata aatggtcaga
    25921 aggattgaga gggagaggag aaagagcata gagccccaaa aggtcaattt agttttcatc
    25981 aaaatcccac tccgcaaggc tagaaaccac atgtgtcggt gcgattggca ggccagctac
    26041 ttcttctgcc ttggtaaaac ctgtcgtcac caagagtgtt ggaatgccat tgtcaatccc
    26101 agcccggata tcagtcaaat aattgtcccc aaccatgatt aactcttcac gttccaaacc
    26161 taagtgctca accgccttgt ccataatgat ggcatttggt tttccgatat aaaccggctt
    26221 cactcgtgtc gctacttcaa gcagcgtaat cagtgagcca gcacctggca aaagaccacg
    26281 ttccgtcggg atgttgaggt caggattggt tccgataaaa tgggcaccct tttgaatagc
    26341 aagagttgct gtggcaaatt tttcatagtc gacttgccaa tccagaccaa ctaccacgta
    26401 ggcagggttt tccttgtctt ccacataacc agccgccttg atggcttcct tgagtcctgc
    26461 ttctccgacg acatagacgg tcttttcaag ccccaaatca ttcatatagt cgatggttgc
    26521 caaagtcgct gtgtagacag tcgatagagg cgtatcgata ttaaaattct gagccaacat
    26581 ctccttaaca ctctctggag tgcgggttgt attgttggtt acaaagagat agggaatgtc
    26641 ccgcttttgc aattcatgaa caaaagtctc tccagcaggg attcggtctt tccccttata
    26701 aatggttccg tctaaatcga ttaaatagcc tttatatttc atctatttct ccctaaacct
    26761 tttttatttc ttgccaagta atgattgctt gggcattgat aaccccatca cttgtaattt
    26821 catgcttgct ttccagtcca gtccgttcaa cagccgatgt aatcacccca cctggtcgaa
    26881 cttccttgac atacttgagg ttgattttct tgggaatata gtgggtcaaa aaatccgctc
    26941 ccatgacctc aaaaatccag tccaagtatt tactgttatt gacatgacca ttcatatcca
    27001 agtcgtaaaa acgaacatgg taatccttgc tgatcggctc ttccaaggat tcatacttcg
    27061 gtccacggat aagtttttta tcaaaatcag actggtaagg agccacaatc tcaggttcaa
    27121 cagcatggac ttttcgactg tcgcggtcca tgagaacaaa ggtcgccatc atatggatga
    27181 gctcctgccc cgcttcattg taaatagtaa agcgacggta gcaaaaaagt cgattgtagc
    27241 tcaaggcttc cgtttcgatg gtaatttctt ccgcaaaacg aggcaaacga accacctcaa
    27301 tatcatattc tgcgataatc cagaccagat tatagtcttc caaaatggcc ttatcactaa
    27361 ctcccagttc aatcgattgc atccctgaaa cttgcagtga cagcaaaatc acatctggaa
    27421 gtttgatatg accgttcata tcagccatat caaaaggaat tttcattttc atttgataag
    27481 ttaagcccat gatcctactc caaaataaat cgttctgcta cagtatctcc caaaaagaga
    27541 cctctctttg tcatgcgaac gtggtcaccc tcaatctgca tgaggccttg ttgaaccaaa
    27601 tctctgacaa tttctccata aagtccagca aaagactgtc caaatttttc ctcaaatcgc
    27661 gccatggaaa ccccggattt cttgcggagt cccaagaaca tttcttcttc catttgctcc
    27721 ttttgactca ggtgatcttc tgtaatacaa gcattgcctt cctcaaccgc actgagataa
    27781 tgacgaatgg gaccatgatt tttatagcgt actccattga cataaccaga tgcccctgca
    27841 ccaataccat agtattcagc attgtcccag tacatgagat tatgacgact ttcaaaaccg
    27901 ggtttggaga aattagaaat ctcataatgc tcaaaacccg ctcgctccag ctctgcaatg
    27961 atgtactcaa acatctccgc ttctagttcc tccttaggca gaggcaattt cccacgtcgc
    28021 atccggttca taaagaccgt atggttttct aaaatcaaac tatacaaact catgtgggga
    28081 atatccaatc caatggcttt agccacattt tcctttactt gctccatggt ctgaccaggc
    28141 agagcataaa tcaaatcaat ggagatgttg tcaaaaccag ccagtttcag gcgatcgata
    28201 ttttcataaa tatccttctc caaatgactg cgcccaatct ttttcaacat cttatcatca
    28261 aaggtctgga cacctagcga aacacgattg acagccgaat ttttcaaaac agctatctta
    28321 tccgcatcca aatcgcctgg attggcttca atggtcaact cttccaagac agacaaatcc
    28381 aagtttttag tcaagccatt cagtaacacc tccagttgcg gagccgacag ggctgtcggt
    28441 gttccaccac cgatataaag ggttgacaac ttttcaatat cataagaacg aaactcttcc
    28501 agcagatgct ctaaatagct gtcgactggc tgatttttga tgaagacctt tgaaaaatca
    28561 caataataac aaatctgggt acaaaatggg atgtgcacat aggctgacgt tggttttttc
    28621 tgcatagtaa ttattatacc acaaagacta gattccagat aaaaatcacc atccccagat
    28681 acatagtccg tccggagatg gtgatggttt attcttctgt tatatgaatc acaatctctt
    28741 ctgagtcatc aagagcttcg gctttttctt gccattgttc cttgagatta tttaattgat
    28801 tttttgatgc ttctgtcgct tgaaaagcat aggatttagc ttgagcaagt atactgtcca
    28861 cagtgatttc acctgactca acctgttctt ttgttttcag aacaaaatct gtagcctgct
    28921 ccttaacttc tgtcagtttt tcacagactt gctccttggc atactccgga tcttctctca
    28981 aatcatctag aaaatcttga gcctgactgc aaacttgttt gcccttatca cttgttaaaa
    29041 acaaggcaag agctgcacct gaaaccgttc ctaaaaggat tgaggataat ttacccataa
    29101 ggattctcct tttttatttt ttgaaaaatt tacttgcaag acgaagagct gacagacttg
    29161 caccagtctt gagtgttttt gaaccagctg atgaagcttt cttgctcaag acacgcgcat
    29221 ggtcattgag gtctgaaaca gatagagata aatctgcaac agcactgaag agtggatcaa
    29281 tcgtagccac cttgacattg atatcatctg ccaagacatt gaccttagcc aacaactcat
    29341 tggtgtgatg caaggtcaca tccacatctg aagtcaaggt tttaatcgtc ttttctgttt
    29401 catcgatgac acgaccaagc ttttgtacag taatgatcag atagaccaaa aagacaatca
    29461 aagctagggc aacaagaata tatgcaactt ctaacattta gttttcctcc tctgtaatat
    29521 agtaaggggc cttctttcga ttttgataaa taacgatcat tataccgaga ccgataagga
    29581 caactgacag ccattgggac actcgaagtc cgaagaacat gagactatct gttcgcatac
    29641 cttcgatgac catacgaccg aaaccatacc aaatcaagta aaaggccgtg atatgacctc
    29701 gtctgagact cttccatttc cgtctaaaaa tcagaatcaa ggcaaagcca agcagattcc
    29761 atagagactc ataaaggaaa gtcggttgac ggtagctccc ctcaatatac atctggtcac
    29821 ggataaagcc aggtagataa tccagattat ccactgttgc accataagct tcttggttaa
    29881 agaaattacc ccaacgcccc aaactttgag caatcataac gctaggcgcc gcaatatcta
    29941 gaaaatccca agtattgatg agtttacggt cagcaaagat atagagcaca agagccccag
    30001 ttatcaaacc accgtaaatg gccaaaccac cattccaaat ggcaaaaatc tctcctaaat
    30061 tctgactata gtaatcaaat cggaaaataa catagtagag acgagctcct aaaatagcca
    30121 agggaaaggc tactaagata aaatctaaaa tatcgtctgg tatgatcttc tttctaggtg
    30181 cttctttcat ggtcaaataa accgcaagaa tcaagcctgt cacaatacat aaggcatacc
    30241 aacgaatggc taggggtcct agttgaatag caattggatc aagcattttg cacctcattt
    30301 cgagcgatga gacttgtcag tcgttcgtcg aacaaacggg tcgcatcaaa gcccatttcc
    30361 ttggcacgat aattcatggc agctgcctca atcacaacag agatattacg acctgtttta
    30421 actggaatac gaatacgagg aatggctacg ccagaaactt caagttcctc tgcattgttt
    30481 ccaagacgat caaaggtctt atgcgtatcg taattttcca aatagacagc aagctgaacc
    30541 tgtgaagaat ccttgacagc actcgcaccg tagagactca taacatcgat aataccaacc
    30601 ccacgaattt caatcaagtg tttcaaaatt tcagctggtt caccccagag agtaatctca
    30661 tccttggcaa agatatcgac acggtcatcg gctaccaaac ggtgaccacg tttgacaagc
    30721 tcaagacctg tctcactctt accaattcca ctatctccct gaatcaagac gcccatccca
    30781 taaatatcca tcaagacacc gtgcacactg gtacgttctg ccaaacgaga atccagatag
    30841 ctagataact ctccagataa acgactggta gctgcacggc tggttaaaat agcaatctta
    30901 cattctctag cagcctttaa catctcctct ggaaccacca aaccacgggc aacaatgact
    30961 gctggtgtct caggtagaaa catttttttc aaaacttcat aacggctgtt ggaaggcatg
    31021 ctgatcagat aagaccactc cttcatcccc aaaagttgaa tccgctctgg tgtatagtag
    31081 tcaaaatagc ctgtcatttc aagaccaggt cgcgtaatat ccgctatatt gatttccttt
    31141 tcaagcaatt ctggttcacc atagacaatg tctagtctaa gcttttcaat cacttctttt
    31201 actaaaaccg acatcatttc ctccttcaaa tgagttcttc tctctattat atcaaattgt
    31261 aggaggaagt ttcagatttt tgcaggcttt ggagtattta tttaaaagaa aaaggactag
    31321 atttctctag cctttcattt caattagaat tttttacgtt tacgagctac ttctgattta
    31381 cgtttacgtt ttacagaagg tttttcatag aattcacgtt tgcgtgtttc ttgaagagta
    31441 ccagctttag taaccgcacg tttgaaacgg cgaagtgcgt cgtcaagaga ttcattctta
    31501 cgtactactg ttttagacat ttttttcact cccttcaagt tcaagatcta ttctatttta
    31561 cacgtaaaag gaataattgt caagaggaaa ctaagttttt tattcttcat aatgattaat
    31621 aatatagttc accatctcct ccatatctgc tttagataaa ggtaattccc agtttgttgt
    31681 ttccatttta agaaaagcat cactgacttt agttattgta tagcaatctt taaaaattgg
    31741 aattttttta gattgttcaa ataagatgcc tattctatca tataagacgt gaagttcttt
    31801 actatttgga tcaatatcaa aagactgtaa agagattaag atgtcatttc ctttatatga
    31861 caaatcaatc cacatgtgat ttgataaatc atatggccta gataatctac tacccgaggt
    31921 gtacttttta tttcctccgc ctcctgaata ttgatttctt gtggtatatg gtttatcttt
    31981 tgacttagta aaaagaaaat tcccctcctt atctttcttt tttgtaatga agcctattat
    32041 actttttctt tcttccgttc tagcactctt atatgcttct tttattttta aaaattcttc
    32101 tttattcatg tgtcctccta aagttaagtt gaagttttat atgtctaaaa ccactcactc
    32161 caaagagcaa gcggttctta tactttttat ttttcgagta agctaagcgc ttcagcatct
    32221 gcgatgatgg ttacatcagg gtgattttgg aggctacttg caggtaggtt ctcagtcact
    32281 gggccagata ctgttccggc aatggcttct gctttcgact caccgtaagc aaaaagaata
    32341 atagacttgg catccaaaat gtttttaatc cccattgaaa tagcttgggt tgggacgtct
    32401 tcaatcttgg taaagaagcg tgcattggct tcgatagtag actggtcaag ttctactaga
    32461 tgcgtttgac tgtcaaatgg agtaccaggc tcattaaagc cgatatgccc attgcgaccg
    32521 attcccaaga tttggaggtc aactggatgg tcagccaaaa tttggttgta gcgttcaact
    32581 tcagcttcag cattgtcctt aacaccacga ggcaagaaac tttctttaaa tggtttttgg
    32641 ttgaacaagt tttcttgcat gaagtaacga taagactgtg gattgtcccc atcaaggccc
    32701 acatactcat caaggttgac actggttaga tttgaaaaat caaggtcact ctcaacaatt
    32761 tccttgtaaa attcaagtgg gctacttcct gtcgcaagtc ctagagtttg agctccatta
    32821 gccaattttt ccttcaaaat ctcaaaagca acttttccac cttgaacttg gttttcaact
    32881 ttaataactt tcatcttttc atcctccatt tctgtctata tttattatat ggtatagacc
    32941 aatttttgtc aagtgaaaac gatttaattt ctaaaaaaag agcgtccaaa cttgaaacgt
    33001 gttataatgg ataggattag aacttagaaa gaatgaacaa atatatgaat acagctgatt
    33061 ttgatttcca cttgccagag gaattgattg cccaaacgcc ccttgaaaaa cgtgatgcct
    33121 ccaaactcct catcgtcaac cgtgagacag gagaaatgca agataaacat ttccactcta
    33181 ttattgatat gctggaacct ggtgatgccc ttgtcatgaa cgacacccga gttctccctg
    33241 cccgcctcta tggtcaaaaa gtggagacag gaggtcatgt ggaacttctc ctccttaaga
    33301 acactagtgg agacgagtgg gaagttctgg ctaaacctgc caaacgcctc aaggtcggta
    33361 ctcgtatcag ctttggtgat ggccgcctca gcgctgtcgt tacagaagaa ttgacccacg
    33421 ggggacgcat tgtccgcttt gaataccaag gaattttcct agaagtcttg gaaagtctgg
    33481 gagaaatgcc tctgccacct tatatccacg aaaaattaga tgaccgtgaa cgttatcaaa
    33541 ccgtctacgc caaggaaagc ggctctgctg cagcaccgac tgctggtctt cacttcacca
    33601 aagaactgct ggcagaaatc caagctaagg gtgttcatct agtctatctg actctccatg
    33661 tcggactcgg aacctttaga cctgtttctg tggataatct ggacgaacac gaaatgcact
    33721 cagagttcta tcaactttct gaggaagctg ctgccaccct tcgctctgtc aaagaaaatg
    33781 gtggtcgcat catcgctgtc ggaaccactt ctatccgcac cttggaaact attggttcca
    33841 agtttgatgg gcaaatccaa gcagattctg gttggaccaa tatctttatc aaacctgggt
    33901 atgagtggaa ggtcgtggat gccttctcaa ccaacttcca tctgccaaaa tcaactctgg
    33961 tcatgttggt ttctgccttt gcaggccgtg aattagtctt agatgcctac caccatgcca
    34021 tccaagaaca ctaccgcttc ttcagttttg gtgacgccat gtttatttat tgagaaagaa
    34081 tttctctaat tcttctaata ccaataaatc gctaagatat tatttcaaag aacatctaca
    34141 attgaaactc tagctagctg tagaagacgc ctagtacatt gaaattaaaa tgcttccccc
    34201 tagcttcgaa aatattgcca tagattgcgt tgactctcca aattgattca tctatatttt
    34261 atttcagctt cctatatttt cttcgctgtt tgtaaatcaa aatgcaagac acatgagtag
    34321 caccatattt gttactctta tctgtcctct caagagacta ttatgagtta tttcagaatc
    34381 attcactact ttgaccctga ctctccttag tctcaaaatc aaagacttat actcttcaaa
    34441 aatctcttca aaccgcgtca acgtcacctt ggattatata tgtgactgac ttcgtcagtt
    34501 ctatctacaa cctcaaagca gtactttgag caacctgcga ctagttttct agtttgctct
    34561 ttgattttca ttgagtatta aacaaaaagt gaacaaatct gaattctaat gtacagaaga
    34621 ctaggcttgt tcactttttt atagtcgcta taagatgacc ttatctatag ctttttatat
    34681 ataattatat attcagacat actattatca attttgtcgc agggaggaat ctgttaacgc
    34741 acccattcac cattatcatt gactctatag ccatctatac ttgtattgac cgctaactca
    34801 cccgatgtat ttacataata ccatttacca ccaacttgga accattgatt gactttcata
    34861 gaaccgttgc tgttgagata gtaccatgaa ccattaactt gtacccaacc tgttgccatg
    34921 gaaccatcag tattataaaa ataccacata ccattttctt gtttccagcc tgttgttgga
    34981 gcaactgctt tagctggttc tactgctaca tctgttcctt ggttagatgt aacagataca
    35041 ggatacgaag gaatagatga ttgctcagga acaacaactt tttcaggttc tctcgtccct
    35101 ctccttatac gtctttttac catctcttta gtaatttgac gagaagtagt ttcttcaatt
    35161 gttccatcac gttcatctac agtatagatt gtagtaagag taatttacca atttctccta
    35221 cttcttctac ttcttgactt ttatcaagag ttgagccatc gagatattct gtttcgattg
    35281 gaatttcttg gacaagaact tggggcttgg ttcctttttt aacaactctt gtttgagagt
    35341 cttctttttg acttaaagta ctctcagtta cttgtccact ctttccatct acatcataag
    35401 ttatcgttgt aactgttttc ccattctttc ctagagtaat ctcttgctcc tgtcctgcag
    35461 aaaggtcatt gtctgcttca tatttagtag caaatggaac aagaacttct tcaaccttgc
    35521 ttttagctgg aactttgata actgtatccg tggcttcttt tctatcaaca gtaacctgtt
    35581 cggtaacatg accagtctct ggattaacat cgtaggtcct tgtcctagtt acatagccat
    35641 cctctccatc aattgtaaca ggattttcac tacggtcttt tgtttcatct ttttcataac
    35701 gaattcgcgt acttgaaatt ttcttgatta ctaccttagg tttagtcgct acttttacaa
    35761 taatatcccc attgtcagcg tcatcatact ctattccctc ttctttatct ctagtatcat
    35821 ctctgacata ttgaatccca tcaacagcat gaacaaaact tgtattcaga ttcctcctaa
    35881 aaataaagtt agcccgatta ccgcagaacc aaaaatcttt ccgagtttac gtaatgcata
    35941 gcgcttatta gtattagatt ttgccattac atcctacttc tagtatagca tcttttctat
    36001 caaacgttaa acaatatatt ttatatatca aatagactta gaatgatata ttgattattg
    36061 aactaacact ttaactatat cgtaatcaat ctcatatata aaggattgca gacatcttat
    36121 ctaaatacat gctaatatat ttagatacaa acattccaac ttgataattt tcactcatct
    36181 ttcatcattc cttatacaac tatgcagtat aaatagaata gttttctcat cagaatgaga
    36241 ctattttaat attagatatt ttgatagaaa aaaacataaa taaaattggt tgaatttaat
    36301 tccctaactc gatagaggat tctattttaa atttaacaac agctaggatt acactataaa
    36361 aagcacatta ccacctgttc ggctggagaa tctagtcgat tagctccctc ttagttacaa
    36421 gcttcaagcg attttttaac taatactaaa ccagattaaa gaagaaacta gaacatctgt
    36481 cttgtactaa tcaatatgct attttaaagt tgcaaataaa cttggaatgc taattattat
    36541 atttacaata aatagtcgac ttcgatagaa acagaaaata gaattcaaca ttcttcccta
    36601 cttataccat aataaataag atccgcaaga tagccttttc tctctacacc attaacaatg
    36661 gtttgtagat aggacattcc agccttttcc atgacacgac ctgaagctgg gttgagactg
    36721 gcataacgtg ctctgacctt ttgaaaacct gcttgagtaa aacaaaagtc caagacagct
    36781 ttcaaggcct ctgtcatcat accatgtccc cagtaagcct tgcctagcac atagccgatt
    36841 tcacagctta aatcagcctc gtctatctta acgatgctga tatctcctat cacttgctct
    36901 gggttttctt ttagacaaat ggcccattta taatagttga gattagtata ggaagcaacc
    36961 caattgcgaa tcgagtttcg agtgatttcg acatcaggat ggggatccca ggtaacatag
    37021 gtcagattct cagcggatga agcccaattt tgaaacatgg cttctgcatc actctccaca
    37081 aatcttctta aaatcaaacg ttctgtctgt aatatttgcg taccgataga tttcatgatg
    37141 ctacctcact tccttatttc tatgctcgta gtaaaaagac tcaactcagt aacagctaga
    37201 aaacgagttt gtcttctaat ttcttgacgc agttgctgca tggtttgaaa tgcgctctag
    37261 caagcttttt caaacctagt catcgttgca ggggtaagac gatgaaatcg aactcttcga
    37321 gttaccgaat ctgcctcatt ttcttatttc taaactcggg gttcaagatc ccccggacct
    37381 tctctcactt tcttatttca agatgagagg ctgaaaacct ccagtggagg tttttcttgc
    37441 tttttaattt cagggcaaga agctaaaaag tcccccggac cttcttcgct ttctcatttc
    37501 taggctcagg gtaaaaacct ccaatggagg tttttactcc caaaagtcat caaatacagt
    37561 gatgggtaag tggcgtttgt gttggccttt gtgccaccag ttttcaatgg tcgcttgagc
    37621 ttctgggctg attgttttgc cttctaggta atcgtcaatc tctgcatagg tgactccaag
    37681 tgcgacttcg tcagctaggc ctggtttgtc ttcttctagg tctgccgttg ggattttttc
    37741 ataaagggct ggctctgcgc caagtttctg caagagttgt tttccttggc gtttattgag
    37801 gcggtaaaga gggagaatat ccgcaccgcc gtcaccaaac ttggtaaaga aacctgtgat
    37861 attttccgcg gcgtggtctg ttccaatgac cgctccgcta tgggaaccag caagggcata
    37921 ctgagcaatc atacggcaac gtgccttgat attccccttg ttgaagtctg aaacaggact
    37981 acctgtcgct tcaactgcag ctgtcatggc atcagctgat tccttgatat tcacaaccaa
    38041 gctgacatct ggctggatga aggctagggc tttttgagca tctgcttcat cagcttgcac
    38101 tccgtatggc aggcggacag cgataaattt gtagctatcg tctcccgttt cagctcgcag
    38161 ttcttccata gctaattgcg ccaaacgtcc tgccaaggtt gaatcttgtc ccccagaaat
    38221 ccctagtaca aaggttttta ggaagggatg ttttttcaga tatcttttta agaaatcaat
    38281 agaacgacgg atttcttcct gggcatcaat cactggtttg acacccagct cttggataat
    38341 cgtttcttgc aaactcattc ttcttctcct tcaccaaggg cttccttgcg catcttatca
    38401 atcaagtcca tcttatcttg ccatacatca cgcgccaaat ccactggata gtgctgcgga
    38461 ttgagcacac gcttatactc atcccacaac ttgtcaaatt ccttacgggc ataatcctga
    38521 atgtcagtca aactaggcaa gttgtaaact aatatacctt ctttgaagat atccaccaag
    38581 agaggaacgg catcaaaatt acgaaccgtc ttcttgatgt atgtataggt cggatggaac
    38641 atcttgattt ctgtcatgtc gctaatatcc acaccatcat aagtgatgta gtcaccttct
    38701 gacttgcctt tttcacgact ggtaatgcgc cacacctgct tcttacctgg cgtcgacact
    38761 ttttcagcat tattagacag cttaatcgta ttgcgcatct gaccagtttc atcttcgatt
    38821 gcaacaatct tgtaaaccgc cccaagagcc ggctggtcat aggctgtaat cagcttggta
    38881 cccacacccc agacatcaat cttggccttt tgcatcttga ggttaaggat ggtattttca
    38941 tctagatcat tagaagcata aatcttagcc tctgtaaatc cagcctcatc cagttgctga
    39001 cggactttct tagaaatgta ggcaatatcc ccagagtcaa tccgcacacc cataaagtta
    39061 atctgatcac ccagctcacg cgccacctga atggcagctg gtacaccgat gcgaagggtg
    39121 tcataggtat ccacaagaaa gacacaattt ttgtgggtcg cagcgtaagc cttgaaagct
    39181 tcatagtcat tgccataaac ctgtaccaag gcatgggcat gggttcccaa aacaggaatg
    39241 tcaaagagct tacccgcacg cacgttgctg gttccattgg cgccaccaat cacagctgcg
    39301 cgtgttcccc agatggccgc atccatttct tgagcccgac gtgtcccaaa ctccatcaag
    39361 ggttcatctt cgataaccga acgaatacga gctgccttcg tcgccaccaa agtctggtag
    39421 ttgacgatgt tcaaaagagc cgtttcgacc aactgacatt gggctagagg tccttccacc
    39481 tgcacaatcg gttcattagc aaaaaccaaa tccccttctt gggcagaacg aacggtcaac
    39541 tccaacttga aattgcgaag gtaatccaag aacgccccat gataaccaag cgactccaaa
    39601 taggctatat cactatctga aaaacgcaag tcttcaagat agttcacaat tctttccaaa
    39661 cctgcaaaaa ccgcatagcc gttcttaaaa ggctgttggc ggaaatacac ctcaaagacc
    39721 gccttcttat tgtgaatccc ttggtcaaag taaacctgca tcatgttgat ctggtacaag
    39781 tccgtgtgca atgtcaaact atcatctgga tacatacttt tcctacttcc ttagctagaa
    39841 acccatgaaa attttcaaga actttcatgt attccaataa attagtacta ttatatcaca
    39901 ttttagctgg attgagaaaa gagtaacaag ctattctcca ctctccaatt catccatatc
    39961 ttgttcaaat tttttctgag cccattcgcc atagctctta agaccaagat tgccaataaa
    40021 gatccacgga aggtaaatga tataagtaat gacccaagca gacaggtatt taaaattcaa
    40081 aggattgtgc tgataaattt ctatgttgaa ttgataattc tgcaacatca aaagagccgt
    40141 aatagccaag gttaggaaaa aacaacccaa aatcgtaaaa tgaaaacgac tatagtaggt
    40201 cactcccaga taacgggcac gattgaaaaa gtaaaatgtc cctatgatga taacgattag
    40261 cagcatatta gaattaaaaa ggcttggtgc taatactgaa atgatataag ataggagcga
    40321 caaagcaatg cagatataga aactttcaga gcccgcttta ttgaacagtt gttcttctct
    40381 ttcgtctagt aattgataat aataaaatct atttttcatc ttcttcctcc caaaatagtt
    40441 ggtctagggt tttccctaaa catctgcaaa tagactggca gagcgagaga ctgggattgt
    40501 attttcccgc ctctattaaa ccaatagtct ggcgtgtcac cccgacagcc tctgccagtt
    40561 gaccttgtgt taaatcacgc tctacccgag ctaattttaa ttttaaattt ttagccacct
    40621 tcgtcctcct tatagtttta atactcatct actcttaaaa aatccaaaac caacacaagc
    40681 tatcagctat ttattataac cccattaatt tgcattccag tattatcttc agccgaaaat
    40741 cagtatttta ggttgtttga aataaacttc ctagtttact cttttatttt tatttagtat
    40801 tagctttagt aagatctttt aaacgaattt ttattgctta tatttcttgt ttagtttagt
    40861 tggtttcata tcactattat ataatgcttt tggaaatgag cctagtataa gtcaaaaacg
    40921 tgttgaatag tgaaagcagg agcataccaa gatgactgat tttttactgt attttaggta
    40981 tgcaaaataa tactaaatga aactaaaatg aatacttctt gcttttcttg agagtgaaaa
    41041 agtacaataa aatgattgta gtgattacca caagatactg actaatagac atcgtatttg
    41101 aagccacaaa tacaggtaaa gaataaaaag gaagaagttc aattgctttg ataaacaaat
    41161 cattactagt attcatcatc atcgtattta atatattagg gataaagagt agtaaagcta
    41221 ctaaaatacc aaataccaag ccacttttca atttgtataa atagaaaatg taagataata
    41281 gaaaataaaa caggactaac aaaattgtat tgactacaat agctactata aaatctaatc
    41341 ctaaatcacc tggaataccc aatttactta ctatgactag taacacaatg ccattcaaag
    41401 cataaataat ggaaactgag atggcatata gaaaattaga caagacgtaa atcatccgat
    41461 tcttctgttg attataaaac aaacttaccg tattgtggga aaaatctcta ctaataactc
    41521 gattagcatg tataacagcc attaacatac cgagcatagc ataaaagttt gaaatatgct
    41581 taataccaag cgatgtctct tgagtcttta gaataaagaa tgtaagcaat atcggcggta
    41641 taaacgagat aagcaaactg agatatatta cgcctgatga atataaattt cttatatttt
    41701 ccttaagaaa attcaattta tttaatttca tgattataac cttcctttat tttgaacgat
    41761 ttaagtaaat atctttaagc gtctcttttt tagtttcaaa atcaactact ttaatagagt
    41821 tttcgttaaa aaatttaaaa agctcactac tttgaatatt cccagacata gtaattctca
    41881 gtccctcttc ctgaacaata tccccaaatt cttgtttggt aatgaaaatg tctctatctg
    41941 tagctgatga aaaagctatc tcaaacaaga aattatgact atcttttcct actttttgaa
    42001 atgtcaaaag cccgctctcc aagaaaagaa ccctctcaca aatttcttca atgtcttcta
    42061 atttatgact cgatattaaa attcccacgt tttcatttaa agctaatttt tttagaaccg
    42121 ctaaaactat ttgtgatgat tcaatatcta aaccattagt cggttcatct aaaatcaata
    42181 tatcaggttt cgtaacgaga gttagaagca aagctaattt ttgttttgta cccaaagaat
    42241 aggtctttac ttttttatta atagactgag tcaaatctaa ctcttggatc aaacttctaa
    42301 atctttcttg attgtagtca acaccatata aatttgacaa atattttaaa ttctctaaac
    42361 ctgttttaga taaaaataat tttggttctt cgattaaata cccaatatta tcactgctta
    42421 taatattacc tgaagtcggt tgattattct gaacaagaat tttcatcaac gtactcttac
    42481 caactccatt tctccctact agaccaacaa tttcaccctt gttaagtgca aaatttatat
    42541 ctttgagaat gattgaatga ccataatgct tacttacatt tataacttac attttttatt
    42601 cctccattaa tattcatggt ttgctctttg attcttattg agtttaaaaa gttgcgcctc
    42661 tttttaacaa cattatatca tatatctaac ttaatgcaat atataaatgt cattttgtaa
    42721 aattttatta acaaaaaact ctctacaaca aaataataga gagttttctg cttattaatt
    42781 tttttcaaaa tatctaaaca tcacttcttt gtttaatttg ataggctttg atacctaagc
    42841 tccgacttta tcacttaaaa aataaaaaag gacagctaat ctcttgcgca tcaagggatt
    42901 tcgccattct ttaatctcgt ttaagtgttg tatatggagc tttgcataat tttttaaaac
    42961 taaacaacta cattaaacac gaactgtgac gctggttcca tcttccttat aaagattgat
    43021 gagtccttct tttaatgcac gaaccatgtc tcctgattga acgggttgag gcaccttaat
    43081 agtcaatagt tccattggat ttggagcgcg gtcgatttta ttgcctttgg catcatgcaa
    43141 atcttcaata taggtttcaa aatgacggaa acctggacca taaaactcaa cttggtcccc
    43201 ttcgttaatg acatttcgtt gacgaatggt tgctgtttgt gccgcatcat cataagaaac
    43261 cacttcagcg acaaacttgt actcagggat tttacggcga gcaccaaaca actgctcatt
    43321 ttcagatggt ataccgtagt agaaacctgt tgccaattca cgttgggcaa ccttccacat
    43381 ctcgtccact aagtcttgtt tgatagcttc aaacttttca gaactttcaa gataggcatc
    43441 cacagccgcc ttgtagcagt tggttactgt tgatacgtag tgaatagact tcatacgtcc
    43501 ttcgattttt agactgtcca caccattttc aatcatatct ggaatgtggt caatcataga
    43561 catgtcaacg gctgacattg aaaattcttc tggaatctca ccctgcaaac tcttacgttc
    43621 tttcccaaat ggcatatcgt aaaggtcgta tttccaacgg catgactgag aacatccacc
    43681 acggttggca tcacgcatac tcatgtggtt tgaaagagta caacgtccag agtatgaaat
    43741 acacatagct ccatggacaa aggcttcaat ttcaacatct gtacgtttgc ggatctcagc
    43801 taattcttcc attgaaacct cacgcgctaa aacgacacga gtcaagccta gctctttcca
    43861 gaactcaagg gtttcatagt tagtggcact ggcttgggta gaaaggtgga tttcaaggcc
    43921 tggtgcttca gtcgctgcaa tcataatcaa ggctgggtca gatacgataa ctgctgcaat
    43981 cccgatatca cgcagtttac ggaaccactc accagcacca gcttcatttc cttcgtgcat
    44041 aaccatatta gccgctacat agaccttggc accatacttg gccgcaaact gcacgccttc
    44101 ttccatctgt tcgaaagtaa agtttcccgc acggctacga agaccatagg cctgaccacc
    44161 gataaagaca gcatctgctc catactgaac agctaccttt agcttctcta aagtccctgc
    44221 aggtgataaa acctcaggac gttttaatgt ttttgtcata atttctccta tttgcaatat
    44281 aaaagtttga catttttcct tctcatttta tagtaaataa aggataaaat caagcctaga
    44341 cggattgttt tgacaattct aatcttttag actttgtata ataactatta aacagtcgaa
    44401 aattacccca gttttaacaa gtagtcaaaa agtctaaaaa agtaaaaacc agtatcccaa
    44461 gatactgact cgtaaaaata tataaattag tccttagatg actactccca ctctgttgct
    44521 cctataatgg aaaaggattg atacagtcta gaatatcgct tttttattta ttattatgat
    44581 ataaaactgt tagaattgat tttttgtttt atacccaaat atgaatagag acgtagctaa
    44641 taagttgaat aacatatttg gaaaaatgag aaatagcaat attactagaa
//
LOCUS       DBERMR010000009        44050 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.009, whole genome shotgun sequence.
ACCESSION   DBERMR010000009 DBERMR010000000
VERSION     DBERMR010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 44050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44050
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.009"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(20..616)
                     /locus_tag="ACL8A1_000578"
                     /pseudo
     CDS             complement(20..616)
                     /locus_tag="ACL8A1_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            complement(833..1057)
                     /locus_tag="ACL8A1_000579"
                     /pseudo
     CDS             complement(833..1057)
                     /locus_tag="ACL8A1_000579"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(1105..1881)
                     /locus_tag="ACL8A1_000580"
     CDS             complement(1105..1881)
                     /locus_tag="ACL8A1_000580"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HGS1201395.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGINALHPEDLIDYQKVFLNHHINCYF"
     gene            2003..2749
                     /locus_tag="ACL8A1_000581"
     CDS             2003..2749
                     /locus_tag="ACL8A1_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGS1201396.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     HQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            2762..3742
                     /locus_tag="ACL8A1_000582"
     CDS             2762..3742
                     /locus_tag="ACL8A1_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HGS1201397.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            3937..4257
                     /locus_tag="ACL8A1_000583"
     CDS             3937..4257
                     /locus_tag="ACL8A1_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGS1201398.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            4303..4611
                     /locus_tag="ACL8A1_000584"
     CDS             4303..4611
                     /locus_tag="ACL8A1_000584"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS1201399.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQSSGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            4628..5926
                     /locus_tag="ACL8A1_000585"
     CDS             4628..5926
                     /locus_tag="ACL8A1_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGS1201400.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASANLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVVTQLLVLVISTVVYFPFFKIQDRLAYQNEIKQS"
     gene            6077..8515
                     /locus_tag="ACL8A1_000586"
     CDS             6077..8515
                     /locus_tag="ACL8A1_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HGS1201401.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            8534..9202
                     /locus_tag="ACL8A1_000587"
     CDS             8534..9202
                     /locus_tag="ACL8A1_000587"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HGS1201402.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEVGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVTQN
                     SRSI"
     gene            9220..10308
                     /locus_tag="ACL8A1_000588"
     CDS             9220..10308
                     /locus_tag="ACL8A1_000588"
                     /EC_number="1.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008888 - glycerol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HGS1201403.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            10763..13264
                     /gene="leuS"
                     /locus_tag="ACL8A1_000589"
     CDS             10763..13264
                     /gene="leuS"
                     /locus_tag="ACL8A1_000589"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HGS1201404.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWPESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSTEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEIPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVAEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVAWLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDETSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDTSIAWSEEGLEGSRKFLDRVYRLITSKEIVTENNGALD
                     KVYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEEKLYVDYAKGFIQMIAPFAPHL
                     AEELWQTVAATGDSISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK"
     gene            13325..14386
                     /locus_tag="ACL8A1_000590"
     CDS             13325..14386
                     /locus_tag="ACL8A1_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HGS1201405.1"
                     /translation="MRTQSHKEIVEEYSASRGSYEDCLNYVENTVKNIIKSQSINVHE
                     IIGRVKTVESLEGKVKRKNYSNLAEITDLCGIRIITYFSDDVDKIAELISQEFEVDVE
                     NTIDKRKSEDPTKFGYVSLHYVVGLKEENTSPTLYGRFKNIKLEIQIRTVMQHAWAEI
                     EHDLGYKSKEDIPDQYRRQFARLAGLIELADDDFVQLKNNINNYEQEIKKKLPTSKKE
                     LPIDSSTLMTYVKEDQNYIELLNTIKSSDMDIDFNIDSSFLSTMIQRAKKLDLRTIGD
                     IAELFEKYKDIFLKVLRIEEDIEPCMSISALTPLLHITNVLESVNNINISDEEFMKVS
                     DDEFSDVFGISYPIDVYEG"
     gene            14613..15308
                     /locus_tag="ACL8A1_000591"
     CDS             14613..15308
                     /locus_tag="ACL8A1_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HGS1201406.1"
                     /translation="MPVNEYGQMIGESMEGYTPGELPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPREMWTYLFQEPVADMEELVSLLNQMLARKDRFYYAIIDRSTGKVLG
                     TFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFEELNYRRYEWKCDAF
                     NLPSRRAAERLGFIYEGTFRQAVVYKGRTRDTDWLSMTDKDWPKVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            15320..15736
                     /locus_tag="ACL8A1_000592"
     CDS             15320..15736
                     /locus_tag="ACL8A1_000592"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1201407.1"
                     /translation="MITIKKQEFVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLAI
                     YVALDGDAVVGLIRLVGDGFSSVLVQDLIVLPIYQRQGIGSALMKEALEDYKDAYQVQ
                     LVTEQTERTLGFYCSMGFEILSTYDCIGMTWINREK"
     gene            complement(16588..16680)
                     /locus_tag="ACL8A1_000593"
     CDS             complement(16588..16680)
                     /locus_tag="ACL8A1_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HGS1201408.1"
                     /translation="MMELILKTIIGPIVVGVILRLVDKWLNKDR"
     gene            complement(17021..17113)
                     /locus_tag="ACL8A1_000594"
     CDS             complement(17021..17113)
                     /locus_tag="ACL8A1_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HGS1201409.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            17247..18245
                     /gene="ruvB"
                     /locus_tag="ACL8A1_000595"
     CDS             17247..18245
                     /gene="ruvB"
                     /locus_tag="ACL8A1_000595"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HGS1201410.1"
                     /translation="MSRILDNEIMGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEA
                     AKMRDEALDHVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELE
                     PGDVLFIDEIHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTR
                     AGMLSNPLRARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPR
                     IANRLLKRVRDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGG
                     PVGLGTLSVNIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            18226..18798
                     /locus_tag="ACL8A1_000596"
     CDS             18226..18798
                     /locus_tag="ACL8A1_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HGS1201411.1"
                     /translation="MNTVKNKQEILEAFRENPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTTVGLRLNEESDFELYAPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            19029..19787
                     /locus_tag="ACL8A1_000597"
     CDS             19029..19787
                     /locus_tag="ACL8A1_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HGS1201412.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            19796..20599
                     /locus_tag="ACL8A1_000598"
     CDS             19796..20599
                     /locus_tag="ACL8A1_000598"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HGS1201413.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKVLLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            20621..21880
                     /gene="rseP"
                     /locus_tag="ACL8A1_000599"
     CDS             20621..21880
                     /gene="rseP"
                     /locus_tag="ACL8A1_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HGS1201414.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            21893..23746
                     /locus_tag="ACL8A1_000600"
     CDS             21893..23746
                     /locus_tag="ACL8A1_000600"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HGS1201415.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTSEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            23846..25225
                     /gene="bglA"
                     /locus_tag="ACL8A1_000601"
     CDS             23846..25225
                     /gene="bglA"
                     /locus_tag="ACL8A1_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HGS1201416.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTVFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            25417..27225
                     /gene="glmS"
                     /locus_tag="ACL8A1_000602"
     CDS             25417..27225
                     /gene="glmS"
                     /locus_tag="ACL8A1_000602"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HGS1201417.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEKYLAGHHFKGQTDTEIAVHLIGKFAEEDGLSVLEAFKRALHIIRGSYAFAL
                     IDSENPDVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDEAGQ
                     VVVDPDIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(27302..27439)
                     /locus_tag="ACL8A1_000603"
     CDS             complement(27302..27439)
                     /locus_tag="ACL8A1_000603"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201418.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEVVDRTDEISNHTYGKATLTWFEEI
                     F"
     gene            27496..28545
                     /locus_tag="ACL8A1_000604"
     CDS             27496..28545
                     /locus_tag="ACL8A1_000604"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HGS1201419.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLTAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRAEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(28645..32415)
                     /gene="spuA"
                     /locus_tag="ACL8A1_000605"
     CDS             complement(28645..32415)
                     /gene="spuA"
                     /locus_tag="ACL8A1_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HGS1201420.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGSVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQDPTPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQASSVKEAVQNESV
                     ENSSKKNIPATPDRQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNKKEN"
     gene            32832..33245
                     /gene="rpsL"
                     /locus_tag="ACL8A1_000606"
     CDS             32832..33245
                     /gene="rpsL"
                     /locus_tag="ACL8A1_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HGS1201421.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            33265..33735
                     /gene="rpsG"
                     /locus_tag="ACL8A1_000607"
     CDS             33265..33735
                     /gene="rpsG"
                     /locus_tag="ACL8A1_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HGS1201422.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            34160..36241
                     /gene="fusA"
                     /locus_tag="ACL8A1_000608"
     CDS             34160..36241
                     /gene="fusA"
                     /locus_tag="ACL8A1_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HGS1201423.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEEIRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            36345..40736
                     /locus_tag="ACL8A1_000609"
     CDS             36345..40736
                     /locus_tag="ACL8A1_000609"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HGS1201424.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVSRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYCSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            40838..41101
                     /locus_tag="ACL8A1_000610"
     CDS             40838..41101
                     /locus_tag="ACL8A1_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HGS1201425.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQVLTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            41094..41372
                     /locus_tag="ACL8A1_000611"
     CDS             41094..41372
                     /locus_tag="ACL8A1_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HGS1201426.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            41393..41545
                     /locus_tag="ACL8A1_000612"
     CDS             41393..41545
                     /locus_tag="ACL8A1_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201427.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            41567..42808
                     /locus_tag="ACL8A1_000613"
     CDS             41567..42808
                     /locus_tag="ACL8A1_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGS1201428.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKANMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            42818..43048
                     /locus_tag="ACL8A1_000614"
     CDS             42818..43048
                     /locus_tag="ACL8A1_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HGS1201429.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            43316..44038
                     /locus_tag="ACL8A1_000615"
     CDS             43316..44038
                     /locus_tag="ACL8A1_000615"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HGS1201430.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
BASE COUNT        13090 a         7763 c         9924 g        13273 t
ORIGIN      
        1 tcttatttca ttttactata ccatcaataa gtgtgaaaag atcttttaca taagaccaag
       61 ccttatcatc tttgtaacca ccttgagctt gtagcatatt tgttaattga gcaaaggcgc
      121 tagaagagtt tgctgggtca gcagttgcga tttttccttt tagttcaggt ttgagaaggt
      181 cgctatatcc ttcgatgttc atgcctttag ttaaatcagg gttgacgatt aaaacactac
      241 catctagtgt ataaggagta gagtagccag ttgtgttttg atattctttg ataacattat
      301 cattttcttt tgaagtatag ttttcaaaga gttctccgtg ggtagcatat tgtgtataag
      361 aaccaccaaa gataacatca gctacaggaa cttctttttc tgactctagt tttttgaaaa
      421 gttctccagt accagcttga atcagttcta ctttgatacc atatttttct tcaaaggcag
      481 gaatagttgc tccaattaag tcctctgagt ttggtcaata aacgactagc gaaccaccgt
      541 ctcctttatc agatgaactg tcatcggcag attcattaga agaacaagca gcataataca
      601 tccatttctt tttcatgatg gatacctccg ttgtgttatt taagtttatt ttaaaacaat
      661 gtaagcgttt ttaaaaccta caattctatt ctatagtata ttgaatctat aacagtacac
      721 tttaactgct aaaatatttc tataaattaa tttgactttc ctgatagaga tgtttacatc
      781 ttatttcaat tcgctatatt agagtaaaat tctctacaaa aagaagaata gcctatttta
      841 ctattcttcc gagtgatttc aatttctttg gggaaatatg gagatacttt ttaaatactt
      901 tggcaaaata tttatagtct gaaaatccaa cttggtcaga gatagcgctt aaagaggagt
      961 tgggagattc agccatctta tctatagctt gtttcaggcg atattgtaag atatattcat
     1021 ttagagtcat ggggaagtct tctttgataa ccttatatag atgcttgtta tctgcaaata
     1081 gctagagcat ttcaaggaaa taaactagaa ataacaatta atatggtggt tcagaaatac
     1141 cttttgataa tcaataagat cttcaggatg aagtgcgttg attccatcca tttcatattt
     1201 ccgatttaat aaacgatatt tgttttcacc aaattgatga aaagggagta gttgaacttg
     1261 gtcgatattt aatgagttaa atagagtagc gaatttttct gcatcctcta aactattgtt
     1321 aaaattagga ataactggga ttcttaaaac gatagtttta ttttgagaaa aagcataatg
     1381 aatgttttta ataatcattt gattaaaaac cccagtcact tttttatgtt ttatagaatt
     1441 ataatgtttt aggtctgtgt agataaaatc cacatattga attaaatcga taaatttttc
     1501 atgatcaaca aaggcagtag tttcaatggc agtgtgtatg tgatgttctt tagctgattt
     1561 taagatggct ttagcaaatt caaactgagc aaatatttca cctcctgata aagttaaacc
     1621 tccaccggat tcttcgtaaa attctttgtc ttttaatacc tctgtaataa tttcttctac
     1681 agtcttttct tcaccgacta aggtgaattt ctctcgttga gcatctttca ttttttcagg
     1741 tttcattctt tgagattcag gattagaaca ccatggacag cgcagaggac atccttttaa
     1801 aaaaacagtt gtacgaatac ccgggccgtc atgaattgaa aagtgttgaa tattaaaaat
     1861 aattccttta gatatttcca taagagttcc tcctcttcat aagtctatcc tatcacaaaa
     1921 acgaaagaaa aacaattacg aacgaaaggt taatttgttg ttttttgctt tttattttgg
     1981 tagaataaaa cggaggtgta aaatgaaaag attggaacaa attattaaat tagtatcaga
     2041 atatgaaaag atcgacgtta atacattatc ggaaaaatta aatgtatcga aagtaacgat
     2101 tagaaaagat ttagataaat tagagtcaaa aggtttatta cacagagagc atggatatgc
     2161 tgtattaaat agtggagatg acttaaatgt acgtttgtca attaattatg aaattaagag
     2221 aaaaattgtt caggaagcag taaaattggt gtcagataat gaaacaataa tgatagaatc
     2281 tggatcgacc tgtgctttac ttgctgagga aatttgcaag caaaaaagaa atgttacgat
     2341 tgtaacaaat tcatttttta tagcaaattt tgtgagagct tatgattcat gtcgtattat
     2401 tgttcttggt ggagagtttc agaaggattc acaggttact gtgggacctt tattaaaaga
     2461 aatgatacag acttttcatg tgcatcaagc ttttgttggg acagatggtt acgataaaga
     2521 gatgggcttt acaggaaaag atttaatgcg cagtgaggta gttcaatata tttcagcagc
     2581 gtcggataaa gtcattgtcc taactgactc aagtaaattt gataaaaggg gtacagtaag
     2641 aagatttgct ttaagtcaag tctatgaagt aataacagac gaaaaacttt ctaaacaaaa
     2701 tatagctaca ttagaaaatg ctgggataat ggttaaggta gtttcgtaag aggttaagtg
     2761 tatgaatcaa gataggaata aactgctctc taaaattgct tatctgtatt atattgaaaa
     2821 cttaaatcag tcacaaatag cagcaaaatt aggaatttat agaacctcta ttagtagaat
     2881 gttaacagaa gcaaggaatg caggaattgt taaaattgaa atagagaatt ttgataccaa
     2941 tatgtttaag ttggaaaatt atgtaaaaga aaaatatggt ctggaaagtt tagaaattat
     3001 tccaaatgaa tttgatgata atccaacaat tttatctgaa agaatttctc aagttgcagc
     3061 aggtgtcctt aggaatctaa ttgatgataa tatgaaaatt ggcttttctt gggggaaaag
     3121 tttaagtaat ttagtagatt taattcacag taaaagtgtc cgaaatgttc acttctatcc
     3181 tctagcaggt ggtcctagtc acatacacgc taaataccat gtgaatacac tgatttatga
     3241 aatgtctaga aaatttcatg gagagtgtac atttatgaat gcaacgattg tgcaagaaaa
     3301 taaattgtta gcagatggta ttttgcaatc aagatatttt gaaaatttga aaaatagttg
     3361 gaaagattta gatatagctg tagtcggaat tggtgatttt agcaataaag gaaagcatca
     3421 atggttagac atgcttacag aggatgactt taaagaattg accaaagtta aaactgtagg
     3481 agaaatttgt tgtcgatttt ttgattcaaa aggtaaagaa gtctatgaaa acttacagga
     3541 aagaacgata gcaatctctt tggaagattt aaaaaatatt cctcaaagtt tagctgttgc
     3601 ttacggtgat acgaaagtat cttcgattct ttctgtcttg cgtgctaatt tagtaaatca
     3661 tttgattaca gacaaaaata caattttaaa agttttggaa gaagatgggg atttgacttt
     3721 tagagagatt ctaggtgagt gaaaatgata gactgattca gtttatcgtt tttcttttta
     3781 gttgattgca catttgtgct tatataaaca aaaatagttt atctgttgtt tttggattga
     3841 caactttatt atgtagttgt attctatagt tacaaaagaa aattttaaaa tttcaaatga
     3901 aaaaagcttt ttacatagtg aaatgaggag gaatttatgg aaattattgt tccagatcaa
     3961 attatcatgg gtttaatttt atatgctggc gatgcgaaac aacatattta taaagcatta
     4021 gattacataa agaacggtac atgtgaacga tgtgaagaag aaatccagtt agctgatgca
     4081 gccttattag aagctcataa tctacaaaca aaattcttgg cacaggaagc gtctggtaca
     4141 aagacagaaa ttacagctct ctttgttcat tcacaagatc atctcatgac tagtatgacg
     4201 gagattaatt taatcaaaga aattattagt ttgagaaaag aacttcataa aaaataatac
     4261 tagagtatta tcattgttat taacatagag gaggaaaaca taatggtgaa gattggtttg
     4321 ttttgtgcag caggtttttc tactggtatg cttgtaaata atatgaaaat tgcagcgcaa
     4381 tctagtggag ttgaggcaga aatagaggcg ttttctcagt ctaaattagc ggattatgcg
     4441 ccaaatatag atgttgcact attgggtcca caagttgctt atacattaga taaatcaaaa
     4501 gaaatttgtg ataagtgtga tgttccgata gctgttattc cgatgatgga ctatggtatg
     4561 ttagatggga aaaaagtatt agatttggcc ctatctttga ttagtgggta agaaaaggag
     4621 atttattatg tcaaagatgg atgttcagaa aatcattgca ccgatgatga agtttgtgaa
     4681 tatgcgtggc attatagctc taaaagatgg gatgttagca attttgccat tgacagtagt
     4741 tggcagtttg ttcttgatta tgggacaatt gccgttcgaa ggattaaata agagcattgc
     4801 tagtgttttt ggagctaatt ggacagagcc gtttatgcaa gtatattcag gaacttttgc
     4861 tattatgggt ctaatttctt gtttttcaat tgcctattct tatgctaaga atagcggagt
     4921 agaggcttta ccagctggag ttctatctgt atctgcattc tttattttgc taagatcatc
     4981 ttatatccct aaacaaggtg aggcgattgg ggacgctatt agtaaagttt ggtttggagg
     5041 ccaaggaatt atcggtgcta tcattatagg tttggtagta ggaagtattt ataccttctt
     5101 tataaagaga aaaattgtta ttaagatgcc agaacaagtt ccacaagcta ttgccaaaca
     5161 gtttgaagca atgattccag catttgtaat tttcttatct tctatgattg tatatatttt
     5221 agcgaagtca ttgactaatg gcggaacatt catagaaatg atttattctg ctattcaagt
     5281 tccgttgcaa ggtttaactg gatctttgta tggtgctatt ggaattgcat tctttatatc
     5341 atttttgtgg tggtttggtg ttcatgggca atcggtagta aatggagtag tgacagctct
     5401 gcttttatct aatcttgacg ctaataaagc tatgttagcc tctgctaatc tatcattaga
     5461 aaatggtgca catattgtta ctcaacaatt tttagattca tttttaattc tatcaggttc
     5521 agggattacg tttggtcttg tagttgccat gctttttgca gcaaaatcaa aacaatacca
     5581 agccttagga aaagttgcag cttttccagc aatatttaac gtaaatgagc cagttgtatt
     5641 tggatttccg attgtcatga atccagttat gtttgtacct ttcattcttg ttcctgtact
     5701 tgcagctgtg atagtatatg gagctattgc aacaggtttc atgcagccat tctcaggggt
     5761 aacattgcct tggagtacac cagctattct atcaggattt ttggtgggtg gatggcaagg
     5821 tgttgtgact cagttattag tattggtgat atctacagtg gtttactttc cattctttaa
     5881 gatacaagat cgtttagctt accaaaatga aatcaaacaa tcttagaggt atttgtgtgt
     5941 tactgttaaa ctcacacatt tgtgctaaaa attagagagt taaaattttt ctagttaaaa
     6001 gcttgaaaat ttctataaaa atcggtatta tattttcgaa agaaataaaa atattttcga
     6061 aagaaaggtg cttacgatgg taaatacaga agtagcaaga acaacaatca agacagaata
     6121 ttttggcagc cttactgaaa ggatgaacaa atatcgagaa gatgttttaa ataaaaaacc
     6181 ttatattgat gctgagagag cagttctagc aacacgcgcc tatgaacgat acaaggaaca
     6241 acctaatgtc ctaaaacgtg catatatgct gaaagaaatt ttggaaaata tgtctatcta
     6301 tattgaagaa gaatctatga ttgcgggaaa tcaagcttct tccaataaag atgctcctat
     6361 ttttccggaa tatacgctag aatttgttct caatgagttg gatctttttg aaaagcgtga
     6421 tggagatgtt ttctatatta cagaagaaac aaaagaacaa cttagaagta ttgctccgtt
     6481 ttgggaaaat aataatttac gtgctagagc tggtgcctta ttacctgaag aagtgtcagt
     6541 ttatatggaa acaggattct tcggtatgga aggtaagatg aattctggag atgctcactt
     6601 agcagttaac tatcagaaac ttttgcaatt tggtttaaga ggttttgaag agcgggctcg
     6661 taaagcaaaa gtagctctag atttaacaga tccagcaagt attgataaat atcattttta
     6721 cgactctata tttatcgtaa tcgatgctat taaagtatat gcaaagcgct ttgttgctct
     6781 tgctaaaagt ttagccgaaa atgcaaatcc taaacgtaag aaagaattac ttgagattgc
     6841 agatatttgc tctagagtcc catatgaacc ggcaactact tttgcagaag ctattcaatc
     6901 agtttggttt attcaatgta ttttacaaat tgaatctaat ggccactctc tttcatatgg
     6961 ccgttttgat caatatatgt atccatatat gaaggctgat ttagaaagtg gtaaagaaac
     7021 agaagatagc attgttgaac gtctgacaaa tctttggatt aagacaatta caattaataa
     7081 ggttcgcagt caatcacata cattttcttc agcaggaagt cctttatatc aaaatgttac
     7141 aattggtgga cagactcgag ataagaagga tgctgttaac ccattatctt atttggtatt
     7201 aaaatcagtt gcacaaaccc atctaccgca acctaatcta actgtacgtt accatgcagg
     7261 tttagatgct cgtttcatga atgagtgtat tgaagtgatg aaacttggtt ttggtatgcc
     7321 tgcatttaat aatgatgaga ttattattcc ttcttttatt gcaaaaggag tattggaaga
     7381 tgatgcttat gattacagtg ccattggatg tgttgaaacg gcagttccag ggaaatgggg
     7441 ctatcgttgc acaggtatga gttatatgaa cttccctaag gttctactta tcacgatgaa
     7501 tgatggaatt gatccggctt cgggtaaacg gtttgcacca agctttggtc attttaagga
     7561 tatgaagaac ttttctgaat tagaaaatgc ttgggataaa acactaagat atttgacacg
     7621 aatgagtgtt attgttgaaa attctattga tttatcattg gaacgagaag ttcctgatat
     7681 tctatgttca gcattgactg atgattgtat tggtcgtgga aaacacctta aagaaggtgg
     7741 agcagtatat gattatatat caggattgca agttggaatt gcaaatttgt cggattcgtt
     7801 agctgcaatt aaaaaattgg tgtttgagga agaacgtata agcccaagtc aactttggca
     7861 tgcactggaa acagattatg ccggagaaga aggtaaggtc attcaagaaa tgttgattca
     7921 tgatgcacct aagtatggta atgatgatga ttatgctgac aaattggtta ctgctgctta
     7981 tgacatttat gttgatgaaa ttgctaaata tcctaataca cgttatggaa gagggcctat
     8041 tggaggaatt cgttattcag gaacatcttc tatctcagcc aacgtagggc agggacgtgg
     8101 aacattagca actccagatg gacgcaacgc gggtacaccg ttagcagagg gttgttcacc
     8161 atcacataat atggatcaac acggccctac atctgtttta aaatctgttt caaaattacc
     8221 aacagatgaa atcgtaggtg gggttctctt aaatcagaaa gtaaatcctc aaacgttagc
     8281 caaagaagaa gataaattaa aactaattgc tttgttacga acattcttta atcgtttaca
     8341 tgggtaccat attcaataca atgttgtttc cagagagacg ttgattgacg ctcagaaaca
     8401 tcctgaaaaa cacagagact taattgttcg tgttgcagga tactctgcat tcttcaatgt
     8461 tctttctaag gcaacccaag atgacattat aggacgtact gagcatactt tgtaaaataa
     8521 agaggttctt tttatggaat ttatgcttga cacattaaat ttagatgaga ttaaaaagtg
     8581 gtctgaaatt ttgccgctag ctggggtaac ttcaaatccc actattgcaa aaagagaggg
     8641 ttctattaat ttttttgaac gaatcaaaga tgtaagagaa ttgattggct ctacaccctc
     8701 tattcatgtt caggtgattt ctcaagattt tgaaggcatc ttaaaggatg ctcatgaaat
     8761 tcgaagacaa gcaggagatg atatatttat caaagtacct gttactccag ctggattacg
     8821 tgcaataaag gtgctaaaaa aagagggcta ccatatcact gcaacagcta tttatacagt
     8881 tattcaggga ttattagcta tcgaagtagg agcggattac ctagctccat attataatag
     8941 aatggaaaat ctgaacattg attcaaattc tgtcattcgt caattagctc ttgctattga
     9001 tagacagaac tctcctagta agattttagc tgcatccttt aaaaatgtag cacaagtaaa
     9061 taatgcttta gctgcaggtg cgcatgctgt tacagcagga gcagatgttt ttgaatcagc
     9121 tttcgccatg ccatctatcc aaaaggcggt tgatgatttt tctgacgatt ggtttgttac
     9181 tcaaaatagt cgttccattt agatagagag gaaatacata tgagaatttt tgctagtcct
     9241 tctagatata ttcaggggga aaatgccttg tttgaaaatg ccaaatcaat tttggatttg
     9301 ggaaattacc cgattctatt atgcgatcag ttggtttatg atattgttgg aaaacgattt
     9361 gaagattatc tacataggta tggtttccat attgttctgg cgctatttaa tggtgaagct
     9421 tctgacaatg aaatcaatcg agttgttgcc ttggctgaga aagaaaattg tgatagtatt
     9481 atcggtcttg gtgggggaaa gacgattgat agcgcaaaag ctattgcaga tttgattgaa
     9541 aagcctgtta ttattgctcc aacaattgca tcgaccgacg cacctgtatc tgctttatct
     9601 gttatttata cagatgaagg tgcatttgat cattatctat tttattctaa aaatccagat
     9661 ttagttttgg ttgatacaaa agttatttca caagccccta agcgtttatt agcgtctggt
     9721 attgcagatg gtttagcaac ttgggttgag gcgcgtgcgg ttatgcaggc aaatggaaaa
     9781 actatgttgg gacaacagca aacattggct ggagttgcaa ttgcgaagaa atgtgaagaa
     9841 acgctgtttg cagatggttt acaggctatg gcagcttgtg aagctaaagt ggtgacacca
     9901 gcattagaaa atattgttga agctaatact ttattgagtg gcctaggttt tgaaagtgga
     9961 ggattagctg cggcgcatgc aattcataat ggttttactg cattgacagg cgacattcat
    10021 catttaacac atggtgaaaa agtagcttat ggaactttag tacaactatt attggaaaat
    10081 agacctaaag aagaacttga taagtatatt gagttttaca aaaaaattgg tatgccaaca
    10141 actctaaaag aaatgcattt ggatcaagtt ggatatgatg atttaataaa agttggtaaa
    10201 caagcaacta tggagggtga gacaattcat cagatgccgt ttaagatttc gccttcagat
    10261 gttgctcaag ctattatcgc tgtagatgcc tatgtaaatt caaaataaac aataaggact
    10321 actgttttcc aaatggtagt cttttattga tcctcgtatt gaattctata gcagattgaa
    10381 ataagatgag aacaaatcga ttgggaaagt aaaattaatt tctataaatg ttttagcaat
    10441 tgtttcgtac tatttcagat tcagtccact atacgttctt cataaatcaa aaagcgacat
    10501 aggttgtcgg ctatttattg tgaatacatt aattagcatt ccagttttat cttcggtcta
    10561 aaataagtat tttgtgctat acgagataag tttcttgact tactccttga tttactgcat
    10621 aacaatggga taaaaagtgg gagatagagc aattcatagt catcaaaatt aatgagatac
    10681 agtatacagt tttttcttta aacacatttc aaattccctc aaaaatggta taatagtaac
    10741 atcacaaaat tggagagaga ccatgagttt ttacaatcat aaagaaattg agcctaagtg
    10801 gcagggctac tgggcagaac atcatacctt taagacagga acagatgcat caaaaccgaa
    10861 gttttatgct cttgatatgt tcccttatcc gtctggagcg ggtctgcacg taggacaccc
    10921 agaaggttat accgcaaccg atatcctcag ccgttacaaa cgtgcgcaag gctacaatgt
    10981 tcttcaccca atggggtggg atgcttttgg tttgcctgca gagcaatacg ctatggatac
    11041 aggtaatgac ccagcagaat ttacagcgga aaatattgcc aacttcaaac gccaaatcaa
    11101 tgcgcttgga ttttcttatg actgggatcg tgaagtcaac acaacagatc caaactacta
    11161 caagtggact cagtggattt tcactaagct ttacgaaaaa ggcttggcct atgaagcgga
    11221 agtgccagta aactgggtgg aagagttggg aacagccatt gccaatgaag aagtgcttcc
    11281 tgatggaact tctgagcgtg gaggctatcc agttgtccgt aagccgatgc gtcaatggat
    11341 gcttaaaatt acggcctatg cggagcgctt gctcaatgac ttagacgagc ttgactggcc
    11401 agagtctatc aaggacatgc aacgcaactg gattggtaaa tcaactggtg ctaatgtaac
    11461 ttttaaagtt aagggaacag acaaggaatt caccgtcttt accactcgtc ctgatactct
    11521 tttcggtgcg actttcaccg ttttggctcc tgagcatgaa ctagtagatg caatcacaag
    11581 cacagagcaa gcagaagcag tagcagacta caaacaccaa gccagtctga aatcagactt
    11641 ggctcgtaca gaccttgcca aagaaaaaac aggggtttgg actggtgctt atgccatcaa
    11701 tcctgtcaat ggtaaggaaa ttccaatctg gattgcggat tacgttcttg ctagctatgg
    11761 tacaggtgcc gttatggctg tacctgccca tgaccaacgt gactgggaat ttgccaaaca
    11821 atttgacctt ccaatcgtag aagtacttga aggtggaaat gtagcagaag ctgcctacac
    11881 agaggatggt cttcatgtca attcagactt cctagatgga ctcaacaaag aagacgctat
    11941 tgccaagatt gtggcttggt tggaagaaaa aggctgtgga caagagaagg ttacctaccg
    12001 tctccgcgac tggctattta gccgtcaacg ttactggggt gagccaattc ctatcattca
    12061 ttgggaagat gaaacttcaa cagctgttcc tgaaactgaa ttgccacttg ttttgcctgt
    12121 aaccaaggac atccgtccct caggtactgg tgaaagtcca ctagctaact tgacagactg
    12181 gcttgaagtg actcgtgaag atggtgtcaa aggtcgtcgt gaaaccaaca ctatgccaca
    12241 atgggcaggt tcaagctggt actacctccg ctatattgat ccacacaata ctgagaaatt
    12301 ggctgatgag gatctcctta aacaatggtt gccagtggat atctacgtgg gtggagcaga
    12361 gcatgccgtt cttcacttgc tttacgctcg tttctggcac aaattcctct atgacctcgg
    12421 tgttgttccg actaaagaac ctttccaaaa actctttaac caaggaatga ttttgggaac
    12481 cagctaccgt gaccaccgtg gtgctcttgt ggcaactgat aaggttgaaa aacgtgatgg
    12541 ttctttcttc catgtggaaa caggagaaga attggaacaa gcgccagcca agatgtctaa
    12601 atccctcaag aacgtggtta acccagacga tgtggtggaa caatacggtg ctgataccct
    12661 tcgtgtctat gaaatgttca tgggaccact tgatacttcg attgcttggt cagaagaagg
    12721 tctggaagga agccgtaaat tccttgaccg tgtttaccgt ttgattacaa gcaaggaaat
    12781 tgttacagaa aacaatggcg ctctcgacaa ggtttataat gaaacggtta aagctgttac
    12841 tgagcaaatc gaatccctca aattcaacac tgccattgcc caacttatgg tctttgtcaa
    12901 cgcggctaac aaggaagaaa aactttatgt agactacgct aaaggcttta tccaaatgat
    12961 cgccccattt gcacctcact tggcagaaga actctggcaa actgttgcag caacaggtga
    13021 ctcaatctct tacgtggctt ggccaacatg ggacgaaagt aaattggttg aagacgaaat
    13081 cgaaatcgtt gtccaaatca aaggaaaagt tcgtgccaaa ctcatggtag ctaaagacct
    13141 atcacgcgaa gaattacaag aaattgctct cgctgatgaa aaagtcaaag cggaaattga
    13201 cggtaaggaa atcgtgaaag tgattagtgt accaaataaa ctcgttaata tcgttgtgaa
    13261 ataattgtaa attatagtta attaaaaaga gagacagttt ttgttcactt tttgggaggt
    13321 attaatgagg actcaaagtc ataaggagat tgttgaagaa tattctgctt cacgggggag
    13381 ttatgaagat tgcttaaatt atgtagagaa tactgtaaag aatattatta aaagtcaatc
    13441 tattaacgtt catgaaataa ttgggagagt aaaaactgta gagtctcttg aaggcaaagt
    13501 aaaaaggaaa aattattcta atttagctga gattaccgat ttatgcggta ttcgtatcat
    13561 cacgtacttt agtgatgatg ttgataaaat agctgagtta atcagtcaag aattcgaggt
    13621 tgatgtagag aatactattg ataaacgtaa aagtgaggat ccaacaaaat ttggttatgt
    13681 ttcacttcat tatgtggtgg gtttaaaaga agagaacact tcacctactt tatatggtag
    13741 atttaagaat ataaaattag agatacaaat taggacagtt atgcaacatg cctgggctga
    13801 aattgaacat gatttgggat ataaaagtaa ggaagatata cctgatcaat acaggagaca
    13861 atttgccaga ttggcgggac taattgaatt ggctgatgat gattttgttc agttgaaaaa
    13921 taatattaat aactatgagc aggagataaa gaagaaatta cctacttcga aaaaagaatt
    13981 accgatagat agcagcacgc ttatgactta tgtaaaagaa gatcaaaatt acattgaact
    14041 attgaatacg attaaatcat cagatatgga tatagatttt aatattgata gttcttttct
    14101 atctactatg attcagaggg cgaaaaaatt ggatttgagg actataggag atatagctga
    14161 attatttgaa aaatataagg atatctttct aaaggtattg agaattgagg aggatattga
    14221 accttgtatg agtatatcgg cattgactcc attattgcat atcactaatg tgttagaatc
    14281 agttaataat attaatattt ctgatgaaga atttatgaaa gtttctgatg atgaattttc
    14341 agatgtattt ggtatatcgt acccaattga tgtttatgaa ggttaaaaag taatttttta
    14401 aatatgaaaa gaggggtttc ctatttgact aagttgaata attcgtagtg ttgctctttc
    14461 taaagtgaag gtaactaaac tcgtcaatat cgttgttaga taagaaataa atccttcaga
    14521 gtagaatctg gaggattttt tgaatcttct tatgaaagta tgatatacta tgggcaacta
    14581 taaagtttga aaagtgaaac aaggagaata agatgccagt aaatgaatat ggtcagatga
    14641 ttggggagtc aatggaaggt tatacaccag gtgaactgcc ttctattgat ttcttagaag
    14701 gacgttatgc tcggatagag gctctctcgg tagaaaaaca tgcggaggat ttgttagctg
    14761 tttatggccc ggatactcct cgggagatgt ggacctacct ctttcaggaa ccagtagcag
    14821 acatggagga actggtcagc cttttaaatc agatgttggc tcgtaaggac cgtttttact
    14881 atgcaatcat agacaggtca actggtaagg ttttgggaac tttttctctc atgcgcattg
    14941 accagaataa ccgagtaata gaagtgggag ctgtcacttt ttctccagaa ctcaggggga
    15001 cacggatagg gacagaagcc cagtatctac tggctcgcta tgtctttgaa gagcttaact
    15061 atcgtcgcta tgagtggaaa tgcgatgctt ttaatctgcc atcaagacga gctgcagaac
    15121 gtttgggctt tatttatgaa ggaaccttcc gtcaggcagt ggtttataag gggcgtacga
    15181 gggatacgga ttggttgtct atgactgata aggactggcc caaagtcaaa gatcgtttgg
    15241 aaacatggtt gcgtcctgaa aattttgata aaaatggaca gcagtacaag agcttgagag
    15301 agctttaaga ggtgttgaga tgattactat taaaaagcaa gaatttgtca agctagagga
    15361 tgttttgcat ctctatcagg ctgtcggttg gacaaattat acccatcaac cagagatgct
    15421 ggagcaggcc ttatctcatt cattagcgat ttatgtggcg cttgatggcg atgctgtggt
    15481 gggcttgatt cgtttggttg gagatggatt ctcatcagta ttggttcagg atttaatcgt
    15541 tttaccaatc tatcaacgtc aagggattgg tagtgcccta atgaaagagg ctttagagga
    15601 ttacaaagat gcctatcaag tccagctggt aacagaacag acagaaagaa ccttgggctt
    15661 ctattgttct atgggctttg aaatcttatc cacctatgat tgtataggaa tgacttggat
    15721 aaacagagaa aaataataaa acttggttgt tcttaagcaa agtttaaggc tggtctagta
    15781 tcatatagtc attaaataaa gacctcctaa ctttatttaa tgaaatccta aaactttttt
    15841 tcatcataat ctcctaatga agtcacctaa tcaggtggct tttttgtagg gtgaggtgat
    15901 gctgatagaa atttttttaa aaatggtata atttgagaaa agttaagcta gttttaagct
    15961 tcgtcttgta tcatatagtt attaaataaa gacctcctaa ctttatttaa taaaatccta
    16021 aacttttctt tttcataata atctccctta actccaccca atcaggtgga gttttttggc
    16081 tctatttcag gcttttgggg actattctaa aaataatttt tcgatatttt tcggtatttt
    16141 tcggattttg gtcggggaat tggcggggac tttttgagat tttggcgggg atttttttag
    16201 cgaatatgac taagaaatag gtcggttgtc gcttcagcaa gttcgtcctc aacttgattg
    16261 taagttgctg ttaattgaag gactggaact gctgtaataa tgattttgct tgtccagtag
    16321 tggcgtatta aatgaggagt gacatataag attgtctctt cgtttactaa atcaaaattt
    16381 ctatgaaatt gatagaatcc ctaactttct cttaaaattg aaagtcttgt tgtaaagcgg
    16441 ttaaagcttg agaagctgag gatgtaaagt tttgacaaat tttgtgaact gtgggataat
    16501 ggagaggaat taaggattag ttctatatca tggactagaa aagaccccaa gctaacttcc
    16561 acattaagct tggggtcttt tttgacacta tctatctttg tttagccatt tatcgactaa
    16621 gcgaaggatg acaccgacca caatcggtcc gatgatagtt ttaaggatta gttccatcat
    16681 gggctatctc acctcctttc gcaagcggtg tagcagtgcc gtagaatatt ataccacata
    16741 agtgctaaac tcgggagtca cccaaccagg tggagttttt tgctctattt caggcttttg
    16801 gggactattt taaacatcat tttccgataa ctttcactta atcttataaa ctttattgga
    16861 cagattctaa agttggagaa gctgaatgga tgaaaatata aaactttgac aaaatgtgtg
    16921 aactatggga taataaaaag gaattgagta ctagttctat atcataggct agaaaagacc
    16981 ccaagctcac gaccaaataa gcttggggtc ttttttgaca ctatttgtcc ttgtttagcc
    17041 atttatcgac tatacgaaga acgacaccga ccacaattgg tccgatgata gttttgagta
    17101 ctaattccat catgggctat ctcacctcct ttcgtaggcg gtgtagcagt gtcgtagaat
    17161 attataccac ataagtgcta aacttaggag tcacccaatc aggtggcttt tttgtttgtg
    17221 gcttggtttt ttgatataat agagccatga gtagaatttt agataatgag ataatggggg
    17281 atgaggagtt agtagaacgc acgctccgtc ctcagtattt acgtgaatat atcggacagg
    17341 ataaggtcaa ggaccagcta caaatcttta ttgaagctgc caaaatgcgg gatgaagcgc
    17401 tggatcatgt gctcttattt gggcctccag gtctcgggaa aacgaccatg gcctttgtta
    17461 ttgccaacga actgggagtc aatcttaagc agacgtcggg tccagtcatt gaaaaagccg
    17521 gagatctggt agctattttg aatgagttag agcctgggga tgtacttttt attgatgaga
    17581 tccatcgttt gccaatgtca gtggaagagg tgctttatag tgctatggag gacttctaca
    17641 tagatattat gattggggct ggtgagggta gtcgtagtgt tcatttggag ttaccacctt
    17701 ttaccttgat tggtgcgacg actcgggctg gtatgctctc caatccgcta cgggcacgtt
    17761 ttgggattac aggccatatg gagtattatg cccatgctga cttgacagaa attgtcgagc
    17821 ggacggcaga tatttttgag atggaaatca ctcatgaggc ggcatctgag ttggccctac
    17881 gtagtcgtgg gacccctcgt attgccaatc gtctcctcaa gcgcgtgcgc gattttgccc
    17941 agataatggg gaatggggta attgatgata ttattaccga taaggctttg actatgctgg
    18001 atgttgacca tgaaggtttg gactatgtgg atcaaaaaat ccttcgtacc atgattgaga
    18061 tgtacagtgg aggacctgtt ggtctaggaa ctctttctgt gaatatcgcc gaagagcgtg
    18121 agacagttga agatatgtat gagccttact tgattcaaaa aggttttatc atgcggacac
    18181 ggtctggacg ggtggcgact gctaaggcat atgagcactt aggttatgaa tacagtgaaa
    18241 aataagcaag aaatcttaga ggcttttaga gaaaatccag atatgatggc cattctgacg
    18301 atcatccgag accttggtct gaaagactcg tggttggcag caggttctgt cagaaatttc
    18361 atttggaatc ttttgtcaga caaatcccct tttgatcatg aaacagatat agatgtgatt
    18421 ttctttgatc cagatttttc ttatgaggaa accttattac tggagaaaaa gctgcgagag
    18481 gattttcctc agtaccagtg ggaattgaaa aatcaggtct atatgcatca gcacagtcct
    18541 cacactgctt cctataccag ttctcgtgat gctatgagta agtatccaga acggtgtacg
    18601 acagttggac tgcgcttgaa tgaagaatca gattttgaac tctatgcacc ttatggtttg
    18661 gaggatattt tgaattttca agttcgtcca actcctcatt tcttagaaaa tgaagaccga
    18721 atggaactct atcaaacacg tttatccaag aaaaattggc aggagaaatg gaaaaatttg
    18781 atttttaaaa atacttaagg aaactttaag ctagggagtg tacactaagt tcataagtta
    18841 agaagacctt aacttaaact cctaaaactt tttcataata atctccctat aaaaataaag
    18901 tcgcccaatc aggcggctta tttttttgaa aaatgggctt ggtgcctgag aataaatagc
    18961 ttagtgatag aagaaaatgg gtaaatatgg tataataaaa cgatagattt ttgaatagga
    19021 ataagatcat gtttggattt tttaagaaag ataaggctgt ggaagtagag gttccgacac
    19081 aggttcctgc tcatatcggc atcatcatgg atggcaatgg ccgttgggct aaaaaacgta
    19141 tgcaaccgcg agtttttgga cataaggcgg gcatggaagc attgcaaacc gtgaccaagg
    19201 cagccaacaa actgggcgtc aaggttatta cggtctatgc tttttctacg gaaaactgga
    19261 cccgtccaga tcaggaagtc aagtttatca tgaacttgcc agtagagttt tatgataatt
    19321 atgtcccgga actacatgcg aataatgtta agattcaaat gattggggag acagaccgcc
    19381 tgcctaagca aacctttgaa gctttaacca aggctgagga attgactaag aacaacacag
    19441 gattgattct taattttgct cttaactatg gtggacgtgc tgagattaca caggcgctta
    19501 agttgatttc ccaggatgtt ttagatgcca aaatcaatcc aggtgacatc acagaggaat
    19561 tgattggtaa ctatctcttt acccagcatt tgcctaagga cttacgagac ccagacttga
    19621 ttatccgtac tagtggagaa ttgcgtttga gcaatttcct tccatggcag ggagcctata
    19681 gtgagcttta ttttacggac accttatggc ctgattttga cgaagcggcc ttgcaggaag
    19741 ctattcttgc ctataatcgt cgtcatcgcc gatttggagg agtttaggag gaaatatgac
    19801 ccaggattta cagaaaagaa ccttgtttgc agggattgcc ctggctattt tcctaccaat
    19861 tttaatgatt gggggcctct tgcttcagat agcaatcgga atcatagcca tgctagccat
    19921 gcatgaactt ttgaagatga gaggtctaga gaccatgacg atggagggcc tcttgaccct
    19981 ctttgcaacc tttgcattga ccattccctt ggagaattac ctgacttttt tgccagttga
    20041 tgggaatgtg gttgcctata gtgttttgat ttcaatcatg ttaggaacga ccgtttttag
    20101 caagtcttat acgattgagg atgcggtttt ccctcttgct atgagcttct acgtgggctt
    20161 tggatttaat gctttactag atgctcgtgt tgcaggtttg gacaaggttc tcttagcctt
    20221 gtgtatcgtc tgggcgacag acagtggtgc ctatcttgtt gggatgaact atgggaaacg
    20281 aaagttagca ccaagggtat cgcctaataa aacccttgag ggtgccttgg gtggtatttt
    20341 aggagcaatt ttagtaacca ttatctttat gatagttgac agtacagttg ctcttccata
    20401 tggaatttac aagatgtcag tctttgctat tttctttagc attgctggac aatttggtga
    20461 tttactagaa agttcgatca aacgtcattt tggtgttaag gattctggga aatttatccc
    20521 tggacatggt ggtgttttgg atcgtttcga tagtatgttg cttgtatttc caatcatgca
    20581 cttatttgga ctcttttaat caaaagacgg aggaaacgct atgctcggaa ttttaacctt
    20641 tattctggtt tttgggatta ttgtagtggt gcacgagttc gggcacttct actttgccaa
    20701 gaaatcaggg attttagtac gtgaatttgc catcggtatg ggacctaaaa tctttgctca
    20761 cattggcaag gatggaacgg cctataccat tcgaatcttg cctctgggtg gctatgtccg
    20821 catggccggt tggggtgatg atacaactga aatcaagaca ggaacgcctg ttagtttgac
    20881 acttgctgat gatggtaagg ttaaacgcat caatctctca ggtaaaaaat tggatcaaac
    20941 agccctccct atgcaggtga cccagtttga ttttgaagac aagctcttta tcaaaggatt
    21001 ggttctggaa gaagaaaaaa catttgcagt ggatcatgat gcaacggttg tggaagcaga
    21061 tggtactgag gttcggattg cacctttaga tgttcaatat caaaatgcga ctatctgggg
    21121 caaactgatt accaattttg caggtcctat gaacaatttt atcttaggtg tcgttgtttt
    21181 ttgggtttta atctttatgc agggtggtgt cagagatgtt gataccaatc agttccatat
    21241 catgccccaa ggtgccttgg ccaaggtagg agtaccagaa acggcacaaa ttaccaagat
    21301 tggctcacat gaggttagca actgggaaag cttgatccaa gctgtggaaa cagaaaccaa
    21361 agataagacg gcaccgactt tggatgtgac tatttctgaa aaggggagtg acaaacaagt
    21421 cactgttaca cccgaagata gtcaaggtcg ttaccttcta ggtgttcaac cgggggttaa
    21481 gtcagatttt ctatccatgt ttgtaggtgg ttttacaact gctgctgact cagctctccg
    21541 aattctctca gctctgaaaa atctgatttt ccaaccggat ttgaacaagt tgggtggacc
    21601 tgttgctatc tttaaggcaa gtagtgatgc tgctaaaaat ggaattgaga atatcttgta
    21661 cttcttggca atgatttcca tcaatattgg gatttttaat cttattccga ttccagcctt
    21721 ggatggtggt aagattgtgc tcaatatcct agaagccatc cgccgcaaac cattgaaaca
    21781 agaaattgaa acctatgtca ccttggccgg agtggtcatc atggttgtct tgatgattgc
    21841 tgtgacttgg aatgacatta tgcgactctt ttttagataa tcgaggaata ttatgaaaca
    21901 aagtaaaatg cctatcccaa cgcttcgcga aatgccaagc gatgctcaag ttatcagcca
    21961 tgctcttatg ttgcgtgctg gttatgttcg ccaagtttca gcaggtgttt attcttatct
    22021 accacttgcc aaccgtgtga ttgaaaaagc taaaaacatc atgcgccaag aattcgaaaa
    22081 gattggtgct gttgagatgt tggctcctgc ccttcttagt gcagaattgt ggcgtgaatc
    22141 aggtcgttac gaaacctatg gtgaagacct ttacaaactg aaaaaccgtg aaaaatcaga
    22201 ctttatctta ggtccaactc acgaagaaac ctttacagct attgtccgtg attctgttaa
    22261 atcttacaag caattgccac tcaaccttta tcaaattcag cccaagtatc gtgatgaaaa
    22321 acgcccgcgt aatggacttc ttcgtacacg tgagtttatc atgaaagatg cttatagttt
    22381 ccacgctaac tatgatagtt tggatagtgt ttatgatgag tacaaagcag cctatgagcg
    22441 tattttcact cgtagtggtt tagacttcaa ggctattatt ggtgacggtg gagccatggg
    22501 tggtaaggat agccaagaat ttatggctat tacatctgct cgtacagacc ttgaccgctg
    22561 ggttgtcttg gacaagtcag ttgcctcatt tgacgaaatt cctgcagaag tgcaagaaga
    22621 aatcaaggca gaattgctca aatggatagt ctctggtgaa gataccattg cttactcaag
    22681 tgagtctagc tatgcagcta acttagaaat ggcaacaaac gagtacaaac caagcaaccg
    22741 tgttgtcgct gaagaagaag ttactcgtgt tgcaacgcca gatgttaaat caattgatga
    22801 agttgcagct ttcctcaatg ttccagaaga acaaacgatt aaaaccctct tctacattgc
    22861 agatggtgag cttgttgcag cccttctagt tggaaatgac caactcaacg aagtcaagtt
    22921 gaaaaaccac ttaggagcag atttctttga cgttgctagc gaagaagaag tggcgaatgt
    22981 tgttcaagca ggatttggtt cacttggacc agttggtttg ccagagaata ttaaaattat
    23041 tgcagatcgt aaggtgcaag atgttcgcaa tgcagttgtc ggtgctaacg aagatggcta
    23101 ccacttgact ggtgtgaacc caggccgtga ttttacttca gaatatgtgg atatccgtga
    23161 agttcgtgag ggtgaaattt ccccagatgg acaaggtgtc cttaattttg cgcgtggtat
    23221 tgagatcggt catattttca aactcggaac tcgctattca gcaagcatgg gagcagatgt
    23281 cttggatgaa aatggtcgtg ctgtgccaat catcatggga tgttacggta tcggtgtcag
    23341 ccgtcttctt tcagcagtga tggagcaaca cgctcgcctc tttgttaaca aaacgccaaa
    23401 aggtgaatac cgttacgctt ggggaatcaa tttccctaaa gaattggcac catttgatgt
    23461 gcatttgatt actgtcaatg tcaaggatga agaagcgcaa gccttgacag aaaaacttga
    23521 agcaagcttg atgggagctg gttatgaagt cttgacagat gaccgtaacg aacgtgtcgg
    23581 tgttaaattt agcgatagtg acttgattgg tcttccaatc cgtatcactg ttggtaaaaa
    23641 agcggccgat ggcattgtag aagttaagat caaggcaact ggtgacacca ttgaggttca
    23701 tgcagataac gtgcttgaaa cgcttgaaat cctaagcaag aaataaaaac tataatcaga
    23761 agaaaaacaa ggaaaaaatg taactagttt ttaccttgtt ttttctgtat aatgggagaa
    23821 atgagtagat aaagaggtaa atgttatgct aagatttcca aaggattttg tctggggatc
    23881 ctctacttct ggaccgcaga cagaaggacg tgtagctggt gacggtaagg gagacaatct
    23941 ctgggattac tggttccaag tggagccaaa tcgctactat aatgggattg gttcagataa
    24001 gacatcgact ttttatgaaa attgggagcg ggatattgag cttttgttag agactggtca
    24061 cacagtcttt cggacttcta ttcagtggtc acggattttt ccacaaggct gtggaaaagt
    24121 caaccctcaa ggtgtggatt tttatcgtaa ggtctttgag gctattaagg ctaaaggaat
    24181 tcgtctgtta gtcaatctct atcattttga tttacctttt gcccttcaag aggatggtga
    24241 tggttgggaa aataaggcga cagtctcagc ctatgaagac tatgctcgtt tttgttttga
    24301 gacttatgga gatttagtgg atcaatggat tacctttaac gagcccatcg ttcctgtaga
    24361 atttggttat ttttatgatg cccattatcc acataaggtg gatgcagagg cagctgttaa
    24421 ggtagcctat catacacaat tggccagcag ccgggctgtt aaggcttgcc atgaactttt
    24481 gcctgattcc aagattggga ttgtcctcaa cttgacaccg gcttatccac gtagccagca
    24541 tcctgctgat gtcaaggcag ctcgtattgc ggcccttttt caggcccaat ctttcttaga
    24601 tccatctgtc ttgggaactt atccacagga gttagtagaa atcttgcatg aacacggtct
    24661 tttacctgat gctacagagg aagagttgga actcattcgt gataatacgg tggacttcct
    24721 tggtgtgaac tactatcaac ctttgcgtgt tatggctcct cgatttgcta agcatccaga
    24781 gagtccactc ttaccagaac atttttacga gccttatgtg atgcctggac gtaaaatcaa
    24841 ttctcaccgt ggttgggaga tttatgagca agggatttat gacatcgccc aaaatatcaa
    24901 ggaaaattat ggcaatattg agtggatgtt gactgagaat ggtatgggtg ttgaagggga
    24961 agaaaaattc cgtcaagatg gaatgattca agatgattac cgtattgact ttgtaaaagg
    25021 tcatcttcgt gaacttcacc gtgccattga agatggtgcc aactgtaagg gctacttgat
    25081 ttggaccttt attgactgct ggtcatggct caacagctat aaaaatcgct atggtttggt
    25141 cgaattagac ttggaaacgc aagaacgtcg tctgaagaaa tcaggccact ggttcaaaga
    25201 attaagcgat aataatggat tttaaaaagg actctgactg attatcccaa tcggtcagag
    25261 ttttttgctt acaggagctt aatttgaact ataccaattt ttactcttga caagtgaaag
    25321 aaacaagtat atactgtttt tgctgattct ataaaagatg aattagacta taccaatttt
    25381 aaggagaaag cacagcttgc ctgtgtcgta tatactatgt gtggaattgt tggtgttgtt
    25441 ggaaacacaa atgcaactga tattttgatt caagggcttg aaaagcttga ataccgtggc
    25501 tatgattctg cgggaatttt tgtcctagat ggtgctgata accatttggt gaaggctgtt
    25561 ggtcgtatcg cagaattgtc tgccaagaca gctggtgttg agggaacaac tggtatcgga
    25621 catactcgtt gggcaactca tgggaaacca acggaagaca atgctcaccc acaccgatct
    25681 gagacagaac gttttgtctt ggttcacaat ggggtaattg aaaactacct tgaaattaaa
    25741 gaaaaatacc ttgctgggca ccacttcaaa gggcaaacag atacggaaat cgccgtacat
    25801 ttgattggaa aatttgcgga agaagacggt ctctcagttc ttgaagcctt taaaagagct
    25861 cttcatatta tccgtggttc atatgccttt gccttgattg actctgaaaa tccagatgtc
    25921 atctatgtag cgaaaaacaa atctccactt ttgattggtc ttggggaagg ctacaatatg
    25981 gtctgctcag acgccatggc tatgattcgt gaaactaacc aatacatgga aatccatgac
    26041 caagagttgg taatcgtcaa ggctgatagt gtggaagttc aagactatga tggaaatcgt
    26101 cgtgagcgtg ctagctacac tgctgaactt gacttgtcag atatcggtaa gggaacttac
    26161 ccttactaca tgctcaagga aattgacgag caaccaactg ttatgcggaa actcatccaa
    26221 gcctacacag atgaggctgg tcaagttgtc gtagacccag atatcattaa ggctgttcaa
    26281 gacgcagacc gcatctacat ccttgcagct ggaacatctt accatgcagg atttgcttct
    26341 aagaaaatgt tggaagaatt gacagataca ccagttgaac ttggaatctc atctgagtgg
    26401 ggctacggta tgccacttct cagcaagaaa ccactcttca tctttatcag ccaatctggt
    26461 gaaacagcgg atagtcgtca agttttggtc aaggctaatg aaatgggaat tccaagctta
    26521 acagtgacaa acgttccagg ttcaaccctc tcacgtgaag ccaactatac catgctcctt
    26581 cacgcaggtc ctgaaattgc cgtggcatca actaaagcct atacagcgca aatcgcagcc
    26641 cttgccttcc ttgcaaaagc agtcggagaa gcaaatggta atgctaaagc gcaagccttt
    26701 gacctggttc atgaattgtc aatcgtagct cagtctatcg aatcaactct ttcagagaaa
    26761 gaaaccattg aagccaaggt tcgtgaactt cttgaaacaa ctcgtaacgc cttttacatc
    26821 ggacgtggtc aagattacta cgtagccatg gaagcaagtc tcaaactcaa agagatttct
    26881 tatatccagt gtgaaggttt tgcggcagga gaactcaagc acggaaccat tgccttgatt
    26941 gaagaaggaa cgcctgtttt ggctctcttg tcagatccag ttcttgccaa ccatactcgt
    27001 ggaaatatcc aagaggtcgc agcccgtggt gccaaagtcc tcactatcgc agaagagaat
    27061 gtagccaaag ataccgacga tatcgtcctt acgaccgtac atccatacct ctcaccaatt
    27121 tcaatggtcg taccaacgca attagtcgct tactttgcaa ccctccaccg tggcctcgat
    27181 gtggataaac cacgtaacct tgctaaatca gtaacggtag aataagctaa aaaagtctag
    27241 ttatctaggc tttttctaat gagcaaattg gttgcttgta cttaagattc gtgttatact
    27301 cttagaaaat ctcttcaaac cacgtcaacg tcgccttgcc gtaggtatgg ttactgattt
    27361 cgtcagttct atccacaacc tcaaaacact gttttgagca acctgcggct agcttcctag
    27421 tttgctcttt gattttcatt gagtattaga ataatttttc aaattgaagg acagagatag
    27481 acaaggagaa tcacaatggt agaattggga atttcaacat ttggggaaat aacggagctt
    27541 gaagggactg gacaaactta cagtcatgct gaacgcattc gccagttggt ggcagagatt
    27601 gagctggctg acaaggttgg tttggacgta tatgggattg gcgagcacca tcgggcggat
    27661 tttgcagtat cagccccaga gattgtcctg accgctgggg cagtcaatac caagaaaatc
    27721 cgtttgacca gtgcagtcag cattctgtca agcatggacc cgattcgttt gttccaacag
    27781 tatgccacta tcgatgcttt gtcaaatgga cgagcggaga ttatggctgg aaggggctct
    27841 ttcacggaat ctttccctct gtttggttat gacttgaaag actacgactc tctttttgat
    27901 gagaaattag acttgcttca gttagtcaat gaaaagacca agttagactg gcaaggtcga
    27961 ttgactcaaa cgattgctgg taaagaagtt tatcctcgtc cagttcagga caaattgccc
    28021 ttgtggatag ctacaggtgg tcatgtcgaa tcaacagtga agattgctca ggctggtcta
    28081 ccgattgtat atgctattat tggtggtaat ccacgttatt ttaaaaagtt gattcaggct
    28141 tatcgtgaga ttggaagcga agcgggccat gctgacaaag acttgaaagt gggagcccat
    28201 tcttgggggt ggattgcgga agatggtgag caggcggtga aagattattt ccatccgact
    28261 aagcaagtgg tggatgctat ttccaaagac cgtccgcact ggcaggaatt gcgttatgag
    28321 caatatttgg agcaggtagg gccaaatggc gccatgtttg ttggcaatcc agatcaggtg
    28381 gccgaaaaat tgattcgcat gatagaggat ttaggattgg accgcttcat gcttcatcta
    28441 ccgcttggtt ctatgcctca tgaccaggtt ctgagagcta ttgaactctt cggaacacaa
    28501 gttgcaccca aagtacgagc ttattttgct atgaaagagg cttaataaaa aatcctaatc
    28561 aagtttatta ggacaacaaa tattgtacga aaattaatcc cccgagctat aatgcttggg
    28621 ggatttttct ataggagggc tagtttagtt ctctttttta ttttttagta agaaaccgag
    28681 accaaggagc gcaaggaggc tgattcctgc aaataggagt ttgttttcgt ttttgattcc
    28741 tgtatttgga agttcagctt gtctatctgg ggttgcaggt atatttttct tgctagagtt
    28801 ttctaccgat tcgttttgaa ccgcttcttt tacagatgat gcttgtgctt cttgtgctgg
    28861 ttgttcagct tgtgaatcag ctgtagcttg gctaggttta ttttccgcat cagctacttt
    28921 ggcatctggt ttagtagaat caggtctagc ttctggagtt gggtcttgat gttcaggctt
    28981 ggaaggggtt gagtctggtt tctcttctgc agttgactca tggctagttc cattttgaga
    29041 gactcgaaga acagtagctg taagggcatt caatttcaag cctttttcag tccattcaag
    29101 tcctttcggg ttggcaattc cgactgatcc tgcttggttt tcatctgcca aaacttccgc
    29161 atttcttaga tgtgcaaagg cagttcccaa attaaattcg cgagcttttt catccgcatt
    29221 gacaaagact gcgtagatat cgccgtttgg agcagtgatt tggtagccaa tcactacatc
    29281 ctctttttcc acaccatttt ggcctgggac agtgatgagg tggacacggt ctttgatatc
    29341 ttggagactc ttaagtcgga aggcatctgt agattgacga agggcaatca aacctttcat
    29401 atagtcacgg ctcttgacat tttcaggata agctttacca tctgtagcct tagtccagtc
    29461 aaacttgttg actgcgtcgc tagaatcgta agaatcatgg atgaagtaag gatagtcaaa
    29521 tgggttgccg tccttatcac gcaacaagtg agatttgttt ggaaccttat cctctgctac
    29581 tggagtcttg taggctgggt cacggaattg tttagtacgt ccatattcct gaccggagtg
    29641 gataaatgga gttccttgag ctgtcaagac catgagattt ccaagtcgta aacgacggtg
    29701 gatttcagca tagttctcag ccttgcttgg gtcttttttg atagactggg caatgatgtc
    29761 aaagagggtc aagttatcat gggctgcgat gtattggatg acatctccag ggctgtcagc
    29821 ttcaaagtta gttggttgag caatgagatt tttaaagatg gtgttgacat cacgcttgcc
    29881 acctgtgata aaggcaggtt gaccttcgtt tggataacca gatttgaggt tgttacggat
    29941 gtcatctgaa aagacagcga cagtatcggt atgtttcatc caatcttggt cagcagcttt
    30001 agtaggcatg ttttcatcac cggcataggt tctccaacct tcgcctaaca tgattagatt
    30061 tggattgagg gcgcgtgcag ccttgtaagc ttcttcgata gaagcggcgt catggtctcc
    30121 catcatatcg aagcggaagc catccacttt gtaggtatca actaggtatt tgatagagtc
    30181 aactaggagc cgtttggtca tatggtgggt tgtccccaag cgtccaccac caaagctagt
    30241 tcgaggtgtg ccatcggcat ccataaagtg gtagtagttt ggctctaggt cttcaaagat
    30301 atcgactttg gctgtgtggt tataaacgac atctaggata gctcccatac cacgtttgtg
    30361 gatttcgttg atgaggtttt taaattctgc gattcgtttt tctggattct taggatcgct
    30421 tgagtacata ccagtcaagg agaagtagtt ttgagggtca tatccccagt tgtagttgct
    30481 gttgcttgaa gcatagtcag acaagcgttc atggtttttc aattcattga caaagtagta
    30541 agacaagact ggaaggagct ggatatgggt tacacccaag tctttgagat agtctagttt
    30601 ttcaatgaag gcttcaaaag tcccaaatgg tttggtcaag tcttttgcaa tggcaggatc
    30661 tgaagtgaag tcacgcacat gagcttcgta gataacggcg tcttcacgag tcttgaaatt
    30721 gtgaatctta ccataagtca agtcttgagg tccgagttta gctggatcta caaaggcggc
    30781 tttagccact ttatgggcat cgtcaatctt ggcatcgtcg ctattccaag cagcaagaga
    30841 tttagcgtaa ggatcgagtg caagaacagt tttaccttga cgctcgattt ggtattgata
    30901 atagtagcca gtgaaatctg tgattccgag tttgtttgtg ctgtctagag tttgtttcca
    30961 agttcctctt tccccttttt cgagagcgac agttccaact actttgtcag ggtcattctt
    31021 gtcgtagaca acaacagaaa ccttatcagc acttggtgac caaagggtca aatcaacttg
    31081 ttttccttct tcttttaggt cagctcccag tttgccatca tagctgtatg tctcatcttt
    31141 caggcgccag cttgttttgg tagtgaattg gtcggaattg tagctaacag tataaggatg
    31201 ttttgtgtca gagaaatctc cgctgtaggt cactttctta ccagcttcat cgattgcaac
    31261 atcggtaata gttaccttgt ttcctaggtg attagtgatg ttggagtgtt tgaggatatc
    31321 ttctttttta gcaccgacaa gtgttgaaaa gctactttca atgctagaag tgcctacgtg
    31381 ttgggctcct gtcatacgga tatcatggac atagtatgga tttgtgtaaa tcgattcatc
    31441 atcgtctttt aggaaaattt ggctatgatt tttcaaatct gtgaacttat aattttcttt
    31501 acggattttc acgtcgtctc cttgtttgct ctcatctagt aataaaaatc caaattctct
    31561 tgcggcttca ttaagaggaa tgtcgatata gcggccatat ttgcctgtag ccgtaaagtc
    31621 tgttccgtca ggccattgag cgctacttgg attttttaca tctccccagt accagagaga
    31681 tttcttgtca tagttgccat ctgtgcggta gtagttgacg cgaacagttc ctgcaggctg
    31741 tggctcgtaa gagaaaacct tgtaatcttg gtctaaccaa gcctcgttca tttttggaac
    31801 tagtttttct acagatttat cgccggttag attttttcca gctgtattgt tgatgaggaa
    31861 gctaattttc ttggcttgtt ctccctttaa tttgacatct aggtaatagc cgtagtcatc
    31921 tttcttggca tccttgaagg acaaagctcc gtttggccag ttttcagatg gtttttcaac
    31981 atcgtcccaa gtccatagtc cttgagcatc cttgttttct tcaggaagtt ttttgacatg
    32041 gatacggaag tagttatctt cgatgggctc ttctgcctta gtttcagttg ttactggact
    32101 agtagaagta gttggctctg ttgcaggttt ttcttctgtt ttctctaggg aagcgtttgc
    32161 atttctaggt gaagaaatat cactttcgtt cttaccagag ccagctgctg tatcagtaag
    32221 aggttgggca agggtggttg tatttctgtt gttgggttcg ttagtggttg gtgtactttc
    32281 gtcggatgag atagttggtg tagccatggc aagcaagaca aggcttgcgc cgataaggac
    32341 agaaccagtt ccatttttga gggaacggat gctgtagacc atttttttct cagtgtgaga
    32401 tggtgttttt ctcataatga ttctccaatc aataaatttc tatatcagta tagcatgtag
    32461 taaaacaata tgcaagcgtt ttctttgatt tgtgaaaaaa attcttactt ttgagaagtt
    32521 ttctgtacta ttttatatat cagtataaat gacggagata aaaatgtcac ataagccaag
    32581 ccgtattatt aggaagatga tgtgttccca gctattttta ttcagaaatt gatctctaac
    32641 attaccaata aaaaagaaaa atatcttgac aagcaaggga aaattttgtt acaataatag
    32701 acggtacttt ttacttttgg tctctcaaga gtgtacaggg acgtgctgac aaatgttgca
    32761 aaagtacaca cagatgatat ctgtcaccaa gtgtatcatc accaaaaata aaaaaacaca
    32821 ggagaatgta gatgcctaca attaaccaat tggttcgcaa accgcgtaaa tcaaaagtag
    32881 aaaaatctaa atcaccagct ttgaacgttg gttacaatag tcataaaaaa gttcaaacaa
    32941 acgtttcttc accacaaaaa cgtggtgttg caactcgtgt tggaacaatg acacctaaaa
    33001 aacctaactc agcccttcgt aaattcgctc gtgtacgttt gagcaacctt atcgaagtta
    33061 ctgcctacat cccaggtatc ggacacaact tgcaagagca cagcgtggtg cttcttcgcg
    33121 gtggacgtgt aaaagacctt ccaggggtac gttaccatat cgtccgtggt gcacttgata
    33181 ctgcaggtgt taacgatcgt aaacaaggcc gttctaaata cggtactaaa cgtccaaaag
    33241 cataaggaaa ggggataaag agaaatgagt cgtaaaaata gagctccaaa acgtgacgta
    33301 ttgccagatc cgctatacaa ttcacaacta gttactcgtc ttatcaaccg cgttatgctt
    33361 gatggtaaac gtggtactgc tgcttcaatc gtttacggtg cttttgagca aatcaaagaa
    33421 gctactggca acgatgcact tgaagtattt gaaacagcta tggaaaacat catgcctgta
    33481 cttgaagtac gtgcacgtcg tgttggtggt tctaactacc aagtcccagt tgaagttcgt
    33541 ccagaacgtc gtacaacact tggacttcgt tggttggtaa caatcgctcg tcttcgtggt
    33601 gaacacacaa tgcaagaccg tcttgcaaaa gaaatcttgg atgctgctaa caacactggt
    33661 gcagctgtta agaaacgtga agatactcac cgtatggctg aagctaaccg tgcattcgca
    33721 cacttccgtt ggtaagatag gatgcgaaag cgttaagaaa gtcccagaga aaatagggaa
    33781 tcgaagcagg ttgcggttgc aaccaatgag attcatcttt ttctccagac ttttagcttg
    33841 agctcaacta aatcatgatg ctaggcacgg taaggatgca aggtgaaaat aggaaactga
    33901 cgcagtattc aacgaataca aggagtttta tctttttcac gcagcatccc gttccagctc
    33961 acatcggcta actaacttta gcccgggttc aaattagcta aatcgattag tattagctat
    34021 aactcagctt accatctcgt aagttgaaac caacaatagc atgaaaacat tgagaacggg
    34081 taggtcctgc ctatccgttt ttattaaaat cgtgttataa tagaatagaa atcaaaaata
    34141 aataggagaa acaaacctca tggcacgcga attttcactt gaaaaaactc gtaatatcgg
    34201 tatcatggct cacgtcgatg ccggtaaaac aacaactact gagcgtattc tttactacac
    34261 tggtaaaatc cacaaaatcg gtgaaactca cgaaggtgcg tcacaaatgg actggatgga
    34321 gcaagagcaa gaacgtggta tcacgatcac atctgctgcg acaacagctc aatggaacaa
    34381 ccaccgcgta aacatcatcg acacaccagg acacgtggac ttcacaatcg aagtacaacg
    34441 ttctcttcgt gtattggatg gtgcggttac cgttcttgac tcacaatcag gtgttgaacc
    34501 tcaaactgaa acagtttggc gtcaagcaac tgagtacgga gttccacgta tcgtatttgc
    34561 caacaaaatg gacaaaatcg gtgctgactt cctttactct gtaagcacac ttcacgatcg
    34621 tcttcaagca aatgcacacc caatccaatt gccaatcggt tctgaagatg acttccgtgg
    34681 tatcattgac ttgatcaaga tgaaagctga aatctatact aacgaccttg gtacggatat
    34741 ccttgaagaa gacatcccag ctgaatacct tgaccaagct caagaatacc gtgaaaaatt
    34801 gattgaagca gttgctgaaa ctgacgaaga attgatgatg aaatacctcg aaggtgaaga
    34861 aatcactaac gaagaattga aagctggtat ccgtaaagcg actatcaacg ttgaattctt
    34921 cccagtattg tgtggttcag ccttcaaaaa caaaggtgtt caattgatgc ttgatgcggt
    34981 tatcgactac cttccaagtc cacttgacat cccagcaatc aaaggtatta acccagatac
    35041 agacgctgaa gaaattcgtc cagcatctga cgaagagcca tttgcagctc ttgccttcaa
    35101 gatcatgact gacccattcg taggtcgttt gacattcttc cgtgtttact caggtgttct
    35161 tcaatcaggt tcatacgtat tgaatacttc taaaggtaaa cgtgaacgta tcggacgtat
    35221 ccttcaaatg cacgctaaca gccgtcaaga aatcgacact gtttactcag gtgatatcgc
    35281 tgctgccgtt ggtttgaaag atactacaac tggtgactca ttgacagatg aaaaagctaa
    35341 aatcatcctt gagtcaatca acgttccaga accagttatc caattgatgg ttgagccaaa
    35401 atctaaagct gaccaagaca agatgggtat cgcccttcaa aaattggctg aagaagatcc
    35461 aacattccgc gttgaaacaa acgttgaaac tggtgaaaca gttatctcag gtatgggtga
    35521 acttcacctt gacgtccttg ttgatcgtat gcgtcgtgag ttcaaagttg aagcgaacgt
    35581 aggtgctcct caagtatctt accgtgaaac attccgcgct tctactcaag cacgcggatt
    35641 cttcaaacgt cagtctggtg gtaaaggtca attcggtgat gtatggattg aatttactcc
    35701 aaacgaagaa ggtaaaggat tcgaattcga aaacgcaatc gtcggtggtg tggttcctcg
    35761 tgaatttatc ccagcggttg aaaaaggttt ggtagaatct atggctaacg gtgttcttgc
    35821 aggttaccca atggttgacg ttaaagctaa gctttatgat ggttcatatc acgatgtcga
    35881 ctcatctgaa actgccttca agattgcggc ttcactttcc cttaaagaag ctgctaaatc
    35941 agcacaacca gctatccttg aaccaatgat gcttgtaaca atcactgttc cagaagaaaa
    36001 ccttggtgat gttatgggtc acgtaactgc tcgtcgtgga cgtgtagatg gtatggaagc
    36061 acacggtaac agccaaatcg ttcgtgctta cgttccactt gctgaaatgt tcggttacgc
    36121 aacagttctt cgttctgcat ctcaaggacg tggtacattc atgatggtat ttgaccacta
    36181 cgaagatgta cctaagtcag tacaagaaga aattattaag aaaaataaag gtgaagacta
    36241 atccgtcctc actctagaag gaagtcactt agtggcttcc ttttgtcttt agaaaatacc
    36301 tctaaatatg gtaaaatagt agaagaataa tgtgaggaaa atgaatgtca aatagttttg
    36361 aaattttgat gaatcaattg gggatgcctg ctgaaatgag acaggctcct gctttagcac
    36421 aggccaatat tgagcgagtt gtggttcata aaattagtaa ggtatgggag tttcatttcg
    36481 tattttctaa tattttaccg attgaaatct ttttagaatt aaagaaaggt ttgagcgaag
    36541 aattttctaa gacaggcaat aaagctgttt ttgaaattaa ggctcggtct caagaatttt
    36601 caaatcagct cttgcagtcc tactataggg aagctttctc tgaaggtcca tgtgctagtc
    36661 aaggttttaa gtccctttat caaaatttgc aagttcgtgc tgagggtaat cagctattta
    36721 ttgaaggatc tgaagcgatt gataaggaac attttaagaa gaatcatctt cctaatttag
    36781 ccaaacaact tgaaaagttt ggttttccaa cttttaactg tcaagtcgag aagaatgatg
    36841 tcctgaccca agagcaggaa gaggcctttc atgctgaaaa tgagcagatt gttcaagctg
    36901 ccaatgagga agcgctccgt gctatggaac aactggagca gatggcacct cctccagcgg
    36961 aagagaaacc agtctttgat tttcaagcga aaaaagctgc agctaaaccc aagctggata
    37021 aggcggagat tactcctatg atcgaagtga cgacagagga aaatcgtctg gtatttgaag
    37081 gggttgtttt tgatgtggag caaaaagtga ctagaacagg tcgtgtttta atcaacttta
    37141 aaatgacgga ctatacttca agtttttcta tgcaaaagtg ggttaaaaac gaggaagagg
    37201 cccagaagtt tgacctcatc aagaagaatt cttggctccg agttcgaggg aatgtggaga
    37261 tgaataactt cacacgcgat ttgactatga acgtacagga tctgcaggaa gttgttcact
    37321 atgagcggaa ggatttgatg ccagaaggtg agcgtcgggt tgagtttcat gctcatacta
    37381 acatgtcgac tatggatgct ttgccagagg tcgaagagat tgttgcaaca gctgctaagt
    37441 ggggacacaa ggcggttgct atcacagacc atgggaatgt ccagtccttt ccacatggct
    37501 ataaggcggc taagaaagcg ggaatccagc tgatctatgg gatggaagcc aatatcgtgg
    37561 aggaccgtgt ccctatcgtc tataacgaag tggagatgga cttatcagaa gcaacctacg
    37621 tggtctttga cgtggaaacg acgggacttt cagctatcta taatgacttg attcaggttg
    37681 cggcctctaa gatgtacaag gggaatgtta ttgctgaatt tgatgaattt atcaatcctg
    37741 ggcatccctt gtcagctttt actacagagt taactggaat tacagatgat catgtcaaaa
    37801 atgccaaacc actagaacaa gttttgcaag aattccaaga attttgcaag gatacggtcc
    37861 tagttgccca caatgctacc tttgacgttg gctttatgaa tgctaattat gagcgtcatg
    37921 atcttccaaa gattagtcag ccagttattg atacgctgga gtttgctaga aacctctatc
    37981 ctgagtataa acgccatggt ttggggcctt tgaccaagcg ttttggtgtg gccttggaac
    38041 atcaccacat ggccaactac gatgcggaag cgactggtcg tctgcttttc atctttatca
    38101 aagaggtagc agaaaaacat ggtgtgaccg atttagctag actcaacatt gatctaatca
    38161 gtccagattc ttacaaaaaa gctcggatca agcatgcgac catctatgtc aagaatcagg
    38221 taggtctaaa aaatatcttt aagctggttt ccttgtctaa taccaagtat tttgaaggag
    38281 tgtcacggat tccgagaacg gttctagatg cccatcgaga gggcttgatt ttaggttcag
    38341 cctgttcaga gggtgaagtt tttgacgtgg tcgtttctca aggtgtggat gcggcggttg
    38401 aggtggccaa gtattatgac tttatcgagg tcatgccacc ggctatctat gcgcccttga
    38461 ttgctaaaga gcaggtcaag gatatggagg aactccagac cattatcaag agtttgatag
    38521 aggttggaga ccgccttggc aagcctgttt tggctacggg aaatgttcac tatatcgaac
    38581 cggaagaaga gatttatcgt gaaattatcg tccgtagttt gggacagggt gcgatgatta
    38641 accgaactat cggtcatggt gaacatgccc aaccagcacc acttccaaag gctcattttc
    38701 gaacgactaa tgagatgttg gatgaatttg cctttttggg agaggaactg gctcgtaaac
    38761 tggttattga aaacaccaat gccttggcag aaatatttga acccgttgaa gtcgttaagg
    38821 gtgacttgta tacgcctttc atcgacaagg ctgaagaaac agttgctgag ttgacctata
    38881 agaaagcttt tgagatttat ggaaatccgc tgccagatat tgttgatttg cggattgaaa
    38941 aagaattaac atccatactg gggaatggat ttgctgtgat ttatctggca tcgcagatgc
    39001 tggtgcaacg ttctaatgaa cggggttatt tggttggttc tcgtgggtct gtcggatcta
    39061 gtttcgttgc gaccatgatt gggattacgg aggtcaatcc tctctctcct cactatgtct
    39121 gtggtcagtg tcagtacagt gagtttatca cagatggttc gtacggttca ggatttgata
    39181 tgccccataa ggactgtcca aactgtggtc acaaactcag taaaaacgga caggatattc
    39241 cgtttgagac cttccttggt tttgatgggg ataaggttcc tgatattgac ttgaacttct
    39301 cgggagaaga tcagcctagc gcccacttgg atgtgcgtga tatctttggt gaagaatatg
    39361 ccttccgtgc gggaacagtt ggtacggtag ctgccaagac tgcctatgga tttgtcaagg
    39421 gttacgagcg agattatggc aagttttatc gtgatgcaga agtagaacgc ctcgctcaag
    39481 gagcggcggg tgtcaagcgg acaacaggcc aacacccggg gggaatcgtt gttattccga
    39541 actacatgga tgtctacgat tttacgcctg tccagtatcc agcagatgat gtcacggctg
    39601 aatggcagac cactcacttt aacttccacg atatcgatga gaacgtcctc aaactcgatg
    39661 tactgggaca tgatgatccg accatgattc gaaaacttca ggatttgtct ggtattgacc
    39721 ctaataaaat tcctatggat gacgaaggcg tgatggcact cttttctggg actgatgtgc
    39781 taggggtaac acctgaacaa attggaacgc ctacgggtat gttggggatt ccagagtttg
    39841 gaacaaattt cgtacgtgga atggtagacg aaacccatcc gacaaccttt gcggaattgc
    39901 ttcagctgtc tggtctgtcc cacggtactg atgtttggtt ggggaatgct caggatctga
    39961 ttaagcaagg aatagcggac ctatcgactg ttatcggttg tcgggacgac atcatggttt
    40021 acctcatgca tgcgggtctg gaacctaaga tggcctttac tattatggaa cgggtacgta
    40081 agggtttgtg gctaaagatt tcagaagagg agagaaatgg ctatatcgaa gcaatgaagg
    40141 ctaataaggt gccagagtgg tatatcgaat cctgtgggaa aattaagtac atgttcccta
    40201 aggcccatgc ggcagcctac gttatgatgg ccttgcgtgt agcttacttc aaggttcacc
    40261 atcctattta ttactactgt gcttacttct ccattcgtgc taaggctttt gatatcaaga
    40321 ccatgggtgc gggcttggag gtcatcaagc gcagaatgga agaaatctct gaaaaacgga
    40381 agaacaatga agcctctaat gtggaaatcg atctctatac aactcttgag attgtcaatg
    40441 agatgtggga acgaggtttc aagtttggta aattagatct ctactgtagt caggcgacag
    40501 agttcctcat cgacggggat acccttatcc caccatttgt agcaatggat ggtctgggag
    40561 agaacgttgc caagcaactg gtgcgggcgc gtgaagaggg agaattcctc tctaaaacag
    40621 aactacgcaa gcgtggtgga ctctcatcaa ccttggttga aaagatggat gagatgggta
    40681 ttcttggaaa tatgccagag gataaccagt tgagtttgtt tgatgagttg ttttaaaaaa
    40741 ttgcttaata atctattaaa agaggctaac gtatatccaa tagatttaca ttagctttct
    40801 tttttgttaa aatagtctat ggaaagaggg tgagagtatg tcaaagatga gtataagcat
    40861 ccgtctggat agtgaggtta aggagcaggc ccaacaggtg tttagtaatc tgggaatgga
    40921 tatgacaaca gctattaata ttttccttcg tcaggcaatt caatatcagg gattaccttt
    40981 tgatgttaga ctagacgaaa atcggaagtt gctccaagtg ttaacggatt tagaccaaaa
    41041 tcgtaatatg agccagtctt ttgaatcagt ctcagatttg atggaggact tacgtgctta
    41101 agattcgtta tcataaacag tttaaaaaag attttaagtt ggctatgaag cgtggtttga
    41161 aggcagaatt attagaagaa gttttgaatt ttctggttca agaaaaagaa catcctgcca
    41221 gatatcgtga tcattcattg acggcatcca agcattttca aggagttcgt gaatgccata
    41281 cccagccaga ttggcttttg gtttataaag tagacaagtc ggaattgatt ttaaatttgc
    41341 tgaggacagg cagtcacagt gatttatttt aatctatttt aagggggttc tcatgaaact
    41401 aagaatattt gcggaagata agccggctaa gaaggtattt gaatatcaat tagaacttgc
    41461 tgatcgtaca attcttctat cgacagcact cttgtcaggt gctattgctt tagcaggaat
    41521 cttttctgct ttgaaagaaa aataaaaata gaaaagagaa aacagaatgg ttttaccaaa
    41581 ttttaaagaa aatctagaaa aatatgcgaa attgttggtt gcgaacggaa ttaacgtgca
    41641 acctggtcac actttggctc tctctattga tgtggagcaa cgtgaattgg cacatctaat
    41701 cgtgaaagaa gcttatgcct tgggtgcgca tgaggtcatc gttcagtgga cagatgatgt
    41761 gattaaccgt gagaaattcc tccatgcccc gatggagcgt ttggacaatg tgccagaata
    41821 caagattgct gagatgaact atctcttgga gaacaaggct agccgtcttg gagttcgttc
    41881 atctgatcca ggtgccttga acggagtgga cgctgacaag ctttcagctt ctgctaaagc
    41941 tatgggactt gccatgaagc ctatgcgtat cgcaactcaa tctaacaagg ttagctggac
    42001 tgtagcagct gcagcaggac ttgagtgggc taagaaagtc ttcccaaatg ctgcgagcga
    42061 cgaagaagca gttgatttcc tttgggacca aattttcaaa acttgccgtg tctacgaagc
    42121 agatcctgtt aaggcttggg aggaacatgc agccattctc aagagcaagg ccaatatgct
    42181 taataaggag caattttcag cccttcacta cacagcgcca ggaacagatt taacacttgg
    42241 tttgccaaag aaccacgttt gggaatcagc tggtgctgtc aatgcacagg gcgaagaatt
    42301 cttgccaaat atgccaacag aagaggtctt cacagcgcct gacttccgtc gtgcagatgg
    42361 ttatgtcact tctacaaaac cgcttagcta caacggaaat atcattgaag gcattaaggt
    42421 gacctttaag gatggacaaa tcgtagatat cactgctgag aagggtgatc aggttatgaa
    42481 agaccttgtc tttgaaaatg cgggtgcgcg tgccttgggt gaatgtgcct tggtaccaga
    42541 tccaagtcca atttctcagt caggcattac cttctttaac acccttttcg atgaaaatgc
    42601 gtcaaaccac ttggctatcg gtgcagccta tgcgactagc gttgttgatg gagcggagat
    42661 gagcgaagag gagcttgaag ctgcagggct taaccgttca gatgttcacg tagactttat
    42721 gattggttct aaccaaatgg atatcgatgg tattcgtgag gatggaacgc gagtacctct
    42781 tttccgtaat gggaattggg caaattaagg agataatatg ttaggaagta tgttcgttgg
    42841 tctcttagtg ggatttttag caggtgctat gaccaatcgt ggagagcgaa tgggatgttt
    42901 tggaaaaatg tttctcggtt ggatcggagc ctttctaggt cacttgctct ttggaacttg
    42961 ggggccagtt ttatcaggaa cagctattat cccagcgatt ttaggagcca tgattgtttt
    43021 agctattttt tggagacgag gaagttaatt tcctaaatct gatactcaat gaaaatcaaa
    43081 gagcaaacta ggaaactagc cgcaggttgc tcaaagcact gctttgaggt tgcagataga
    43141 actgacgaag tcagctaaaa acactgtttt gaggttgcag atagaactga cgaagtcagt
    43201 aacatatata cggcaaggcg acgttgacgc ggtttgaaga gatttttgaa gagtatgaaa
    43261 cgaaaaggag gttggtcatt gtactagcct ccttttgata tgatataata gttctatgag
    43321 attagataaa tttttagttg cctgtgcggt gggaagtcgg actgaggtca aaaacttgct
    43381 caaggctggg cgcgtgactg taaatggtaa aaaagaaaaa tcagctaaat tgcagattga
    43441 tgaaaaaata gatgagattc gctttgatgg gcaagtgttg gagtatgaag agtttgtcta
    43501 ctacatgatg aacaagccca aaggagttat ctcagcgact gaggatccca agcacagaac
    43561 cgttctggac ttgttggatg acttggcgcg gagtaaggaa gttttcccag taggacgctt
    43621 ggatattgac acgcatggtc ttttgctctt gaccaatgat ggtcagctgg ctcatgttct
    43681 tctttcgccc aagcgtcatg tggacaagac ttatctggca caagtcaagg gaatcatgac
    43741 ccaagaagat gtggagacat ttgctgaggg tattcctctc aaagacttta cctgtcaacc
    43801 cgctatactg gagcttgtat ccatagatac agaaaagaat caaagccaaa tccgtgtgac
    43861 cattgcagaa gggaagtttc atcagatcaa gcgtatggtg ggctactgtg gcaaggaagt
    43921 ggtggacttg caacgattga ctatgggaac tttagtatta gatgagaacc tggaacgagg
    43981 ggaatggcgt cgcttgacca aggaagaatt agaaattctc cgcgctaata ttatttaatt
    44041 tagtttaata
//
LOCUS       DBERMR010000010        42521 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.010, whole genome shotgun sequence.
ACCESSION   DBERMR010000010 DBERMR010000000
VERSION     DBERMR010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 42521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42521
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.010"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..498)
                     /locus_tag="ACL8A1_000616"
     CDS             complement(<1..498)
                     /locus_tag="ACL8A1_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HGS1201431.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRK"
     gene            complement(535..663)
                     /locus_tag="ACL8A1_000617"
                     /pseudo
     CDS             complement(535..663)
                     /locus_tag="ACL8A1_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267199.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CD1845 family protein"
     gene            complement(719..1588)
                     /gene="rsmI"
                     /locus_tag="ACL8A1_000618"
     CDS             complement(719..1588)
                     /gene="rsmI"
                     /locus_tag="ACL8A1_000618"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HGS1201432.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFDS
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQGVEEKDEEDLFVEIQTRIQQGVKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            complement(1591..1908)
                     /gene="yabA"
                     /locus_tag="ACL8A1_000619"
     CDS             complement(1591..1908)
                     /gene="yabA"
                     /locus_tag="ACL8A1_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HGS1201433.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            complement(1948..2838)
                     /locus_tag="ACL8A1_000620"
     CDS             complement(1948..2838)
                     /locus_tag="ACL8A1_000620"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HGS1201434.1"
                     /translation="MKQDQLKAWQPDQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            complement(2835..3473)
                     /gene="tmk"
                     /locus_tag="ACL8A1_000621"
     CDS             complement(2835..3473)
                     /gene="tmk"
                     /locus_tag="ACL8A1_000621"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGS1201435.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            complement(3589..4386)
                     /gene="proC"
                     /locus_tag="ACL8A1_000622"
     CDS             complement(3589..4386)
                     /gene="proC"
                     /locus_tag="ACL8A1_000622"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HGS1201436.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSELFCQLLSKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLSRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            complement(4390..5652)
                     /locus_tag="ACL8A1_000623"
     CDS             complement(4390..5652)
                     /locus_tag="ACL8A1_000623"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HGS1201437.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASILPRLDQ
                     MLVAERKEAGLEPIQFRLDSKASQFVSGQAAETQDFDTEFLDYVLAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEVAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            complement(5662..6771)
                     /gene="proB"
                     /locus_tag="ACL8A1_000624"
     CDS             complement(5662..6771)
                     /gene="proB"
                     /locus_tag="ACL8A1_000624"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HGS1201438.1"
                     /translation="MKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAMLHEAGHEL
                     ILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQIVSAQILL
                     TQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSAQVAAMVQ
                     ADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTGGMLTKIK
                     AATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLAFYAQSQG
                     SIWVDKGAAEALSQHGKSLLLSGIVEAEGAFSYGDIVTVFDKESGKSLGKGRVQFGAS
                     ALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            complement(6872..8755)
                     /gene="cbpE"
                     /locus_tag="ACL8A1_000625"
                     /gene_synonym="pce"
     CDS             complement(6872..8755)
                     /gene="cbpE"
                     /locus_tag="ACL8A1_000625"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HGS1201439.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDNRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRQDGLVNISTSYKPIPS
                     FQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNNRWFY
                     LTDSGVAAKNWKKIAGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTDGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            complement(8758..9645)
                     /locus_tag="ACL8A1_000626"
     CDS             complement(8758..9645)
                     /locus_tag="ACL8A1_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HGS1201440.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLALAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            complement(9635..10096)
                     /gene="lspA"
                     /locus_tag="ACL8A1_000627"
     CDS             complement(9635..10096)
                     /gene="lspA"
                     /locus_tag="ACL8A1_000627"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HGS1201441.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRASQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            complement(10093..11001)
                     /locus_tag="ACL8A1_000628"
     CDS             complement(10093..11001)
                     /locus_tag="ACL8A1_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGS1201442.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKSQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLREGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            11030..11182
                     /locus_tag="ACL8A1_000629"
     CDS             11030..11182
                     /locus_tag="ACL8A1_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGS1201443.1"
                     /translation="MIPYFRSFPKRKKEHPILLHYLPFSSQPYFQNQFKKISGYTPLQ
                     YRKEKR"
     gene            complement(11234..11905)
                     /locus_tag="ACL8A1_000630"
     CDS             complement(11234..11905)
                     /locus_tag="ACL8A1_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGS1201444.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETVEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(11959..12180)
                     /locus_tag="ACL8A1_000631"
     CDS             complement(11959..12180)
                     /locus_tag="ACL8A1_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HGS1201445.1"
                     /translation="MILMTKNINLTNEELELIQGGADPYGKEPNGYYPWQMEPVLTMP
                     VHGFCPRGTDDLGYIGGGNHLCKGSAARF"
     gene            12453..13262
                     /locus_tag="ACL8A1_000632"
     CDS             12453..13262
                     /locus_tag="ACL8A1_000632"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGS1201446.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLELDLQNEYIIVNNGCSTHQTSDWSLVDWKELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(13443..14318)
                     /gene="aguB"
                     /locus_tag="ACL8A1_000633"
     CDS             complement(13443..14318)
                     /gene="aguB"
                     /locus_tag="ACL8A1_000633"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HGS1201447.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGGVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(14328..15413)
                     /gene="aguA"
                     /locus_tag="ACL8A1_000634"
     CDS             complement(14328..15413)
                     /gene="aguA"
                     /locus_tag="ACL8A1_000634"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047632 - agmatine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HGS1201448.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRSF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAELVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEKRYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(15410..16537)
                     /gene="nspC"
                     /locus_tag="ACL8A1_000635"
     CDS             complement(15410..16537)
                     /gene="nspC"
                     /locus_tag="ACL8A1_000635"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0045312 - nor-spermidine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HGS1201449.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCHILQYVQEEVGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDADLEELLGITDHIVF
                     NSERQLRKHGSRCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFEAQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(16537..17796)
                     /locus_tag="ACL8A1_000636"
     CDS             complement(16537..17796)
                     /locus_tag="ACL8A1_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HGS1201450.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            complement(17793..18653)
                     /gene="speE"
                     /locus_tag="ACL8A1_000637"
     CDS             complement(17793..18653)
                     /gene="speE"
                     /locus_tag="ACL8A1_000637"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HGS1201451.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQSFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGSFMLPKYVE
                     DILEEEEGKK"
     gene            18909..19514
                     /locus_tag="ACL8A1_000638"
                     /pseudo
     CDS             18909..19514
                     /locus_tag="ACL8A1_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846390.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            19494..19619
                     /locus_tag="ACL8A1_000639"
                     /pseudo
     CDS             19494..19619
                     /locus_tag="ACL8A1_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(19591..21066)
                     /locus_tag="ACL8A1_000640"
     CDS             complement(19591..21066)
                     /locus_tag="ACL8A1_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGS1201452.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTDKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSILLIGEQMNSEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEKVIELSEYARREINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDDSVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
     gene            21773..22822
                     /locus_tag="ACL8A1_000641"
     CDS             21773..22822
                     /locus_tag="ACL8A1_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HGS1201453.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASLLGKAPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVKKSSLTKELKEKILHYHNQ
                     KFSPEMMVIAKGVNVGISTIYYWIHRGKLGLSKQDLLYPRKGKALKKQASTNFKPAGQ
                     SIEQRPEAINLRLENGHYEIDTVLLTRAKNYCLLVLTDRKSRHQIIRLIPNKSAEVVN
                     QALKLILKQHKILSITADNGTEFNRLFDVFSEEHIYYAHPYASWERGTNENHNRLIHR
                     WLPKGTKKMTPKEVAFIEKWINNYPKKCLNYKSPREDFLMANLNLKFSVRNKSRNRFK
                     FCRSFKYPARRCSWC"
     gene            22764..23438
                     /locus_tag="ACL8A1_000642"
     CDS             22764..23438
                     /locus_tag="ACL8A1_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HGS1201454.1"
                     /translation="MDSNFAGRLNILRAGVLGANDGIISIAGVVIGVASATTNIWIIF
                     LSGFAAILAGAFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYAAYIQN
                     GECKTSAQLLTNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPML
                     SVTIFPSEYRIPATVLIVGVALLLTGYTSARLGKDPTRTAMIRNLAIGLLTMGVTFLL
                     EQLFSI"
     gene            complement(23480..25468)
                     /locus_tag="ACL8A1_000643"
     CDS             complement(23480..25468)
                     /locus_tag="ACL8A1_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1201455.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTITYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVLFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQMKVVITVLL
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVTDPLTALTTFFLAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSAESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYKIKEKEVLRYTYFAVANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFNKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVDASEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSNLADASSQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(25470..26228)
                     /locus_tag="ACL8A1_000644"
     CDS             complement(25470..26228)
                     /locus_tag="ACL8A1_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1201456.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPTRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFNEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            26656..26847
                     /locus_tag="ACL8A1_000645"
     CDS             26656..26847
                     /locus_tag="ACL8A1_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201457.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFISTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            complement(27201..27473)
                     /locus_tag="ACL8A1_000646"
     CDS             complement(27201..27473)
                     /locus_tag="ACL8A1_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HGS1201458.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVLIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(27531..27980)
                     /locus_tag="ACL8A1_000647"
     CDS             complement(27531..27980)
                     /locus_tag="ACL8A1_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HGS1201459.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPIKLYRCQSCQQTYQRKRRIDTKRYRCGLCRGKLLLVNQPED"
     gene            complement(27967..30096)
                     /locus_tag="ACL8A1_000648"
     CDS             complement(27967..30096)
                     /locus_tag="ACL8A1_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HGS1201460.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
     gene            30376..30507
                     /locus_tag="ACL8A1_000649"
     CDS             30376..30507
                     /locus_tag="ACL8A1_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HGS1201461.1"
                     /translation="MAVKFTKRDDLDKMFEEFAKLPDLKQVTFPDDKEKKAKAEKKN"
     gene            30508..31413
                     /locus_tag="ACL8A1_000650"
     CDS             30508..31413
                     /locus_tag="ACL8A1_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HGS1201462.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSIVVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSHLSPAKKVFQVFVQAIDEFFDTGRYLVFGCLFASVIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLAYSLLIGVIL"
     gene            31410..32225
                     /locus_tag="ACL8A1_000651"
     CDS             31410..32225
                     /locus_tag="ACL8A1_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HGS1201463.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWITAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(32354..33070)
                     /locus_tag="ACL8A1_000652"
     CDS             complement(32354..33070)
                     /locus_tag="ACL8A1_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGS1201464.1"
                     /translation="MKKYFIGGLGSNAYHSKDFLQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEEFICLIGHSLGGDLARYLASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIVLDLDVLTSKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNEATWREEALKELPDYFDTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(33074..33385)
                     /locus_tag="ACL8A1_000653"
     CDS             complement(33074..33385)
                     /locus_tag="ACL8A1_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201465.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEHPELYAVAQTSGEIPEKECEELRQIITTIFEQKMNKHSI
                     K"
     gene            33528..33885
                     /locus_tag="ACL8A1_000654"
                     /pseudo
     CDS             33528..33885
                     /locus_tag="ACL8A1_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(34018..34890)
                     /locus_tag="ACL8A1_000655"
     CDS             complement(34018..34890)
                     /locus_tag="ACL8A1_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HGS1201466.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            35714..35797
                     /locus_tag="ACL8A1_000656"
                     /pseudo
     CDS             35714..35797
                     /locus_tag="ACL8A1_000656"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
     gene            36129..36308
                     /locus_tag="ACL8A1_000657"
     CDS             36129..36308
                     /locus_tag="ACL8A1_000657"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGS1201467.1"
                     /translation="MQKANTKLEKPINQPLSSHIFRHTLLSTLAEKNAIMVRVEHKNA
                     KTVNNIYTHVSKRME"
     gene            complement(36641..38146)
                     /gene="pyk"
                     /locus_tag="ACL8A1_000658"
     CDS             complement(36641..38146)
                     /gene="pyk"
                     /locus_tag="ACL8A1_000658"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HGS1201468.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(38205..39212)
                     /gene="pfkA"
                     /locus_tag="ACL8A1_000659"
     CDS             complement(38205..39212)
                     /gene="pfkA"
                     /locus_tag="ACL8A1_000659"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HGS1201469.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            complement(39295..42396)
                     /locus_tag="ACL8A1_000660"
     CDS             complement(39295..42396)
                     /locus_tag="ACL8A1_000660"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HGS1201470.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDISLKLPRFNPARPAVEELRERAELGFVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLDLFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDSALEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSIEAGHTAEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNYSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKSIYIGLKSIKGLSKDLALWIIEHRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
BASE COUNT        12593 a         9413 c         7721 g        12794 t
ORIGIN      
        1 ttttctgatt ttagttctga aagttccaaa aggtgcaacc gtatttacat gaataaactt
       61 atacacttcc caagtcgctg ttttagtcgc ttcatcagcc cactttctca tatgcggttc
      121 aaataattct tcctcactca acgaatcaat cattgcagag atagaattaa tattttcatt
      181 taattttgct ttcaactctt gcagagataa atgagcgtag gtatctgtga accactgata
      241 taattcacca agttgattcc atttaaattt atccgatggt gtttttactt gaagcccctt
      301 tctttcatct tcttcccatt taagaaccaa gttggtccaa ccaacctgat aagcaaggtt
      361 ttctgctgga gttctgtcaa cttcatcagc tctcttatct tttaaatttt ctggaatatt
      421 atcaaattct aaaatatatt tctcaaatgt tttctctatc tcagctttta gttcttcttt
      481 attttcgtat gttcgcaaaa aatcaccttc tttctgattt atcaatcctt tgatttaaaa
      541 tgaccttatt tttctattta taccttctag gaacgctata actgctgctc caaccatcgg
      601 tattccgtat gggcttaata aaaaacttaa taccaaagct tctataccaa gtgatacatc
      661 tttatattat catcactaat taagttaaac agacagaatt attacatcct gtctcccttt
      721 attgtttttc ttcccagtcg tggtaggcag cgtagagctg acttttattc cactggtaaa
      781 tcttagcgac ttccttgata gcttggtttt tcttcacacc ttgctggatg cgggtttgaa
      841 tttctacgaa caagtcttcc tcgtcctttt cctccacacc ctgactggca ccctcaacaa
      901 tgagaagaca ttcgcccttg agtggcgttt cagcaatgct ttctaataac tcagagatag
      961 tacctcgttg gtattcttca tagattttgg tcaattccct gaccaagaca acggagcggt
     1021 caccgtagac ttctagcata ttttccaatg tatctgccac acgatggggc gattcataaa
     1081 aaatctgagt ttcaggatag tcttttttcg agtcgaaaaa ttgcttttgc tggcctgatt
     1141 ttctcggtaa aaagccgtaa aagatatgtg gctgcggcgc taaaccactg gcaatcaagg
     1201 cggaaattcc tgcagaggca cctggaactg taacaactgc aatttcttcc tcaatagctg
     1261 ccttaaccaa atcatgacca gggtctgaaa tgctaggcaa accggcatca gagacctgag
     1321 caatactttg ccctgctttc aagaaaccaa tcaaatcagg aattttttcc ttggcattgt
     1381 gctcatgaaa actgatctgc ttggtggaaa tgtcaaaatg cttgagcaaa agacctgtat
     1441 tgcgcgtatc ctcagcagca atccaatcta cttctttcaa ggtctggata gctcgaaaag
     1501 tcatatcatc tagattgcca atcggcgttg ccactagata cagcttgcca tagggagact
     1561 gccccttaaa acttttttga atctgcatgc ctactccctg tatagcaact cgtcacaaaa
     1621 catacattcc tcgtcctgct ctcgacgttg tccataaaaa tcattacata cgtgaaatcc
     1681 atcacggtaa atacgacgga cactttcgcg aacatgcttg gccttgacgg gagcgtctgc
     1741 ttccacctca cccaagcgtt ctcgcaactt actattttcc aagcgaagag ctgtattttc
     1801 ttctaccaag ctcttgagat ttttcttgat ggcttccaca tcggctaagg ttaccaataa
     1861 ttgttgggaa aaatcatcca gcgcgtcaaa taattctttt ttgtccataa aacagccctt
     1921 tcctttcttt atcattcatt tttgagttta tatttctttc aagaccagat attccatggc
     1981 attttgaaag ctgacattag cttgccacat ttttctagct tctagtaaat cttgtagaat
     2041 cactcttact cttacctgca agaggtcctg cccacagaga acttcaagaa tccgtaaaac
     2101 ctgatcctgt ttttccttat catctgccaa gttggctaat ttggcaacct gtagataact
     2161 ttcttttttc ttagctacta accaagtcag caggcgttca ctttcatcga ccaaggtcca
     2221 aaaacttgcc tgattagcca acttttctgc ttcagctcgc gattgactaa acttagctaa
     2281 aagagtcgct tttttcttaa caagtcccat ttgttctaag agtaagataa gtttttcttc
     2341 ttgcttttta aagtggaaga tctgagtccg acttcggatt gtcggtaaca tcttttcctc
     2401 atcgctagtc aagaagaaaa tataaacttc actctggggt tcttcgatga ccttgagcag
     2461 agaattggct gcgttgggat gcattttatc cgcttgctcg atgataaaga cctgttgctg
     2521 gctttcaatc cctgcttgag aaaactgtcc caccaattcc cgaatgcgtt ctgtcttgat
     2581 gacctgattg actggcttaa tcaaggtgac atctggaaac tcttcctgtt caatcagctt
     2641 gcaacttcgg catttctcac atggtaagac gccaacttta tccgtacaaa agaggctctt
     2701 agctaaaaat tgcgccattt ccaagcttcc aaagaaacct gaaaagagat aggcgtgatt
     2761 gagctggtct tgttctaaga tacggacaaa acggtcaaac tgatctggtt gccaagcctt
     2821 tagttgatct tgtttcattt ggccaagccc attccgtcaa acaagacagc cttggtagtt
     2881 tccacaactt gctccaaagg gagactagca tcaatcttga caatgcgatt tccctcttta
     2941 tccagaagag aaaggtagcc ttgacgaact tttttatgca agtccaaccc ttccaaatcc
     3001 aaacgattaa cctcgcggtc actattagca gcaatacgag ccagcccttc ttccacctcg
     3061 atgtcaaaat agagtgtcaa atcgggtttg agaccatctg tcgcaaactg attgagccag
     3121 tcaatggctt caatatctaa gccacgacca aatccctgat aggcaacaga actatcgata
     3181 aaacgatcca tgatgaccaa cttgccagct tcaagggctg gaagaacttt ttccaccaaa
     3241 tgctgtctgc gactggcaat atagagaagt agctctgttt tagcatccat ctgagtatga
     3301 cttggatcca aaatcacttc ccgaatcttc tccccaatca agactccgcc aggttcacgg
     3361 gtcgtcaaca cctctactcc tttttcctct aaaattggta gcagagcctc taaaacactg
     3421 gtcttgcctg ctccctctgg tccctcaaga gagactaaaa atccttttga catgtctaac
     3481 tcatttcttt tttactactt ctattctatc aaaaaaatag gggtttgtga caatcttttt
     3541 gtgtcttctc atttcagcca ccataaaaga ggcagtcaaa gctacctctt atttacctag
     3601 ttcttgtgtt cgtttatagg cttgatgaac tgcctccatg actgtgcctc gaaaagcatg
     3661 cgcttctagg cttgctacgc cagcaatagt cgaaccgcct ggactacaaa cttgatcttt
     3721 caaaactcca ggatgttgct ggctttctag gaccaattgc ccagctccta ccacagtttg
     3781 ggctgccatt ttcaaggctg tttctcgtga taatcccgtc tgaacacctg catctgccaa
     3841 ggcttcaata aagagataga caaaggctgg tccacagcct gcaagacctg tcgctgcatt
     3901 gattaagctt tctcctaatt caaccaagag accagccttg gataaaagct gacaaaagag
     3961 ctcactgtcc tcagccctgc aattaggaga caaggcataa ctaatcactc cttgcccgat
     4021 agaagcaggg gtattaggca tcatacgaat aattcggtgt tgacttggga taagacttgc
     4081 tagtttttct aaggtcaatc cagctgccat cgaaatcaaa agaagacttt ctcttttttc
     4141 aaggatggtc tggtattgag aaagcagttc agaaaactga gcaggcttaa ctcctagaaa
     4201 aatcacatct gcttctgcaa acatttcttc attgctggaa gcctgaccac caaagtctgc
     4261 aatgaaagca tctaccttag cttgactacg attggcaagg agaatctcat ctgacgtcct
     4321 agtctgcaag acagattttg ccaagctagc acccatattc cccaaaccga taaatccaat
     4381 cttcatctct tactccctta tctgcccatc accagcaacc acatacttgt agctggtcaa
     4441 ctctttcaag cccattggac cacgcgcgtg caatttctga gtagaaatcc ccatttcaca
     4501 accaagacca aattgtcctc catctgtgaa acgagttgag gcattaacat acaccgctgc
     4561 agagtccact tgatctgtaa agtatgctgc aacttcagca ttttccgtca caatagcatc
     4621 cgaatgatgg gtgctgtggg attcaatgtg cgcaaccgct tcttctaaac tgctcacaac
     4681 cttaacagca aggacatagt ctaaaaactc ggtgtcaaag tcttgggtct cagctgcttg
     4741 acctgaaaca aactggcttg ctttgctatc caagcggaat tgaattggtt ccagtccagc
     4801 ttccttacgc tctgcaacca gcatttgatc caagcgagga aggatgcttg ctgccttgtt
     4861 ttcatgaacc agcagaacct ccatggcatt acaaacagaa ggacgactgg ttttagcatt
     4921 gttgatgata gacagcgcct tgtcttcgtc tgcatcctta tccacataga catggacaat
     4981 cccagtccct gtctcgataa caggtacaat cgcattctca accactgcat tgatcaagcc
     5041 agctcctcca cgaggaatga gaaggtctag atagcccttg gccttcatca tagcataact
     5101 actttcacgg ctagtatcct ccaccagttg aatcacattt ggatgaatag tagtcgtctc
     5161 caagcccttc ttcaaggctg tgacaatggc atgggttgtt tgataggcat ccttaccact
     5221 acgaagaaca accgcatttc cactcttaag agtcaaagca gccgcatcag acgtcacatt
     5281 tggacggctt tcatagataa taccgatgac acccatagct acacgttttt ttgtgataac
     5341 caaaccattt tcaagctgac ttgtttctaa aacttcaccg attggatctg gtaaggcaac
     5401 cacttcacga attcctcttg ccatcgcttc tatacgatct gcatccaaat aaagacgatc
     5461 caacatcaca tctgagattt tccccttagc cgctgccata tcgagggcat tagccgctaa
     5521 aatttcctca gtagcagcca ctaagtgatc agccatggct agcaaggctt ggtttttcac
     5581 ttcttcacta gctgtgttaa tcgatttttt aacagcctgt acctgttcaa attgttcttg
     5641 tctactcacc atagtttacc tctaaaattc tgtaaaaagt agttggattt caggagtaat
     5701 ggaaatccag tcgtcacggt aaatcaagac acccttggct ttttgagaac gcaacatatc
     5761 ctccaaagca gatgctccaa attgcacgcg tccttttcca agtgattttc cactttcctt
     5821 gtcaaatact gtcacgatat caccgtaaga aaaggctcct tctgcttcaa cgataccaga
     5881 taagagaaga ctctttccat gttgagagag agcttccgca gcccctttat caacccaaat
     5941 agaaccttga ctctgagcat agaaggcaag ccattgtttc tgggtacgaa gccccttctc
     6001 ttgagcaaca aagtaagaac catcctcggt ctcctctgcc gcctcaatca tggaatctga
     6061 tttcaaggat gagcagatat aaacaggaac tcctgattcc gtcgcgatag ttgcagcctt
     6121 gattttggtt aacatacccc cagttccgtt tgacgaacct gctccaccag ccatatcaat
     6181 aatctcacga ttgatggtct cgattctctc caagcgtttg gctcttggat ctgaattagg
     6241 atttccagta tagagaccgt ccacatctgt caagaaaact aaaaggtctg cttggaccat
     6301 cgccgctact tgagcactta gagtgtcatt gtccccaacc ttgagctcat caataacgac
     6361 actatcattc tcattgatga taggaattgc cccacggttg agcaaaaccg acaaagcctg
     6421 atgggcattt ttataacgac gcttatccac aaagtcatct tgggtcagca agatttgtgc
     6481 agaaacgatt tgacgcaaga gaagattggt cgtatattct tccaacaaaa gtccctgccc
     6541 taccgctgct gaagcctgtt tatcagcaat tttagtcgga cgctttttaa atcctaaggc
     6601 tccaaaacca gccgcaatgg cacctgaaga caccaaaatc aactcatgac cagcctcgtg
     6661 cagcatagcc aactgctggg taatatcctt taccttacta cgtgataaac ttccatcctc
     6721 atttgtcaga gaagaagtac ccaccttaaa gacaatccgt ttgtatttca tagtctcact
     6781 ccgattattc atatttatcc attataacat gaataacagg aaatagaaaa gagttgatag
     6841 gaaaaagaag gccctaaaac cttcttttct actactgttc tgattccgat ttgtttacac
     6901 tcgtttcaga gctttgtgaa gttgaggatg tcaaatcttg actagttgaa gaatttgtaa
     6961 cactttcatg ttgactcgtt tctttatttt caagatcctt tttcactact tctttggatt
     7021 cttttatttc tgaatgactt gtagttttaa ttgtagcaga tttctcgatc ggaacatcca
     7081 ctaaccaggc accacttgaa tccacagtat agccttctgg tgtcttacca ttcacaagta
     7141 attgaccatt ttctttcaag aagtaccatt tatccttgtc tttaatccaa cctatagctc
     7201 gtgaaccatc tgtcttgtag aaataccaat catttgcctt tttaagccag ccttgcttca
     7261 tagctcctga ttcttctaga tagtaatgat gtccagcaac ttctatctca cctgtcttca
     7321 tgataccagt agaatccata tagtaccagg cttctttatc ttttacccag cccgttttca
     7381 tttcccctga tggagcaaag taataccatt gccccttata ttgaagccaa cctgtcttca
     7441 tagagccatc aacatccaaa taataccact gctctttatc ttgaatccaa ccaatttcca
     7501 tctggttttc tttgttaaaa taataccaga ttccagcgat tttcttccaa tttttagcag
     7561 caacaccaga gtctgtcaaa tagaaccaac gattgttcca ttttttccat tgattctgta
     7621 ataagatacc cgtttggtta aagtaatacc attcaccctt aatttcattc caaccgacag
     7681 catattctcc tgtagaatca ggcgcttgat accaccagtt tccatatgaa ctcttatgcc
     7741 aaccagcttg aaaacttgga atcggcttgt aggaagttga aatgtttact aaaccgtctt
     7801 gtcgaatatc aaaaactgtc gcatcatagt ctttgctggc tgcgttgatt ctctcaattc
     7861 ctcgttcttt gagccaatta acatactcac tatcaacacc atttttccaa ggtagactat
     7921 ccgaagtttg aacaatcaaa ctcggactca aatttttaat gaaatccttg gtatttgatt
     7981 tgttggtatc atggtgatga ttaaacttca tcaaatcaac ttttccaatg agaggaccat
     8041 acttgtcttc tgctccatga acattatcta aatcgccccc aaggtaaatt ttcttgccat
     8101 tgactttcac cacgctaatc aaggaattgg aattgtcatc ccaaattttc tttaattcac
     8161 ccgatgaatc agtttcattt tcataattat agagctgaat atccatgtcc ccaaactgaa
     8221 aatgagcatc cccttgtgtg atattttgaa taactgaaac acctttttct gcagcagtct
     8281 gtaaaacctt atcatagcca tacagattat cccatagacg ttcagaatta gtaatacgat
     8341 tatcactata ttttttaaga tagactcggt caactggata ggtagacagt aattcatcaa
     8401 catttccaat atgatcactg tgggtatggg tcaccaaaat aaaatcaagt ttttggacac
     8461 ccaattcctt caaacgacga aagacacggt ctgttagaac atgcttataa gacgtttcaa
     8521 ttccttctct ccatggatag cgagaatcac ttccatctgg gaaatcataa tcttctcctg
     8581 tatccaccat ggcaaaatgt ccattgcttt caagaataat cgcatcactg ccaccttctt
     8641 gaacattgat aaagtggatt ttatttcctg aactttcttg agcctgaaca ttcccatacc
     8701 atgacaaacc taaaaaagcg cctacaagtg ctaaactagt taatttcttt ttcattctta
     8761 ctttctcaat ctctccaagg tttccttaaa aatctctggg atatctgctt taaattccaa
     8821 ggtcttacct gttctcggat gagtaaaacc taaagtcttg gcatgaagaa attgtccatg
     8881 tcctttcaaa gtcttgcgag gaccatagac ctcatcacca gcgactggat ggccgatata
     8941 agccatgtgg acacggattt gatgagtgcg ccctgtctcc agttgcaact ctactaagct
     9001 ataatcgcca aagcgttcca agacgtgaaa acgcgtcact gcaggcttcc ctttagcagt
     9061 tacagcctgt ttcttacggt ctttttcact ccggccaatc ggcgcttcaa ttacaccacg
     9121 atcattaggc agatttccat gaacaatcgc ccaatatttg cggagagact ttttatcctt
     9181 gagttcttgg gcaagtgcta gatgcgcatc atcgttttta gcaatcatga gaagacctga
     9241 cgtatcctta tcaatacggt gaacaattcc cggacgcaga accccattga taccagacaa
     9301 gtccttaata tgatacatga gggcattgac tagagttcca ctggtatgac cagcactcgg
     9361 atgcacaacc attccctgag gtttgttgac aacagctaca tcctcatctt ggtagactat
     9421 ttctagcgga agatcctcag ccacatactc taatacctct ggttctggca catggtaagt
     9481 gacgacatca ccctcttgga ctgtgtattt agctttcttg acttgaccat tgaccaagac
     9541 ctggcctgat ttaatttgtt cattcgcgag actacgtgat aattctgaca aatctgacaa
     9601 agccttatcc aaacgcagac caccagtttc aattttaatt tccatttatt tcctctttta
     9661 gcattgcaat caataaaata atcactccaa ccgtcagata gctatctgcc acattgaaaa
     9721 ttgcaaagtt gataaagtca aggtggaaca tatccacaac aaagccctga ctggccctgt
     9781 caataaagtt tccaagacca cccgcgatta tcaaagtcaa acccaagacc atccagaatg
     9841 agtcctccat gtgtttatgt aaataccaaa tggcacctat cacgacaacc agagtaatga
     9901 cagcgaataa cagctgctga tcttgtaaga tagaaaaggc tgcacctcga ttttgcaggt
     9961 aggtcaagct aacgaaattg ggaatccagg agcgcacttc acccagtgga atctgctgga
    10021 cgatatagga tttgaccaac tgatccagcc caattaaaag cagtacaatg actgccacta
    10081 ttgctctttt tttcatgatt tcctcttttg atcaaaatat tcttgcatga cttctacgaa
    10141 gagagtccca gcttgactaa gctccacttc ttcacgttta acatagacca tgcggttatc
    10201 taggttatcc ttgagacgaa taactgtaat accattaaca ctgtcactat ctaaaaatcc
    10261 agaacctgtc gcataggcgt ccgtccgctc caaaatacca ttcaaggtgg cacggtctgt
    10321 cacattaaac atctgtgagc tagcgctggt atcgacaaag ttctctgaat aataaaggta
    10381 ctcgtctttc tcttgagtga aacgaaccgt tggtaaatcc gctaaatcct ccatgactaa
    10441 ttcctctttc tgggctaaag gatgaccctc acggagataa atatgggtat ggaaaggaat
    10501 caattcgatg acctccagac ctaatttttc aacccgttgc ataatcccct ttttattttg
    10561 attgttgagg tagataatcc caatctcact atgcccttgc gccacttcat ctaatatttg
    10621 aacagtagtt gattcaaaaa tacggaagtt cttatagtca ggatagcgct ctgaaaaggc
    10681 cgtaatagtt ggtggcaaga agtcatagtg ctggctagca acagaaaatt catctttttc
    10741 ttcttcagga ttggcatact gattttgaaa aatatcaaat cctttaacca attcttgcga
    10801 tttttcatag aattccatcc cacgacgggt caagaaagtc cctgagctgg tccgacggaa
    10861 aatcttaaaa cccaactctt tttccaaatc acgaacagaa atagacagac tcggctgact
    10921 aacatacatc ttttcagcag cttcacggaa agtaccacta ttggcaatgg caacaacata
    10981 gcgtaattgt tgaatgttca tcttctaccc ctaacttctt tattttttca ttataccata
    11041 ttttagaagt tttccaaaaa ggaaaaaaga acatcctatt cttcttcact atctgccttt
    11101 ctcaagccaa ccttattttc aaaatcaatt caaaaaaata agtggctaca caccacttca
    11161 gtatcggaaa gaaaaacgtt gactatttgt gaaaaaagaa aatgccggaa agttccgaca
    11221 ttattttagt aagctaactt cctgaaaata ggagcaacca cacgtcccag aaggcagtga
    11281 tattaatgag aatatgaact agtattggat agtagagatt tttagtcatg cgatacaata
    11341 gagctaaaat aagacctcca ctagcataaa tgaaaaaagc aagaggagtt aaagcaaaat
    11401 tgatatgaat ataaccgaaa ataatagcag aaagcaaaac atctccgtac caaggtgagt
    11461 ttttgaaaaa ggttgtcata agcacacctc gataaatcaa ttcctcagca ataggggcga
    11521 tgaagcaaac gatgagcaag aaataaggga actcctgtct ccccatcatt tctaccgttt
    11581 cattcaaaga aatctgattt gaagacaggg atatgaaata cgaaaagagg aagtcagaca
    11641 tatatgaaat gatgtagccc agtaaaaggt agatgaagta cctcagctgc cacttgaaat
    11701 gaaaaatttt ctttcctgca aaaaccagta gatagatgac cgctaagaaa agaactccgc
    11761 tctccatcaa aagcagaatc tgaaaaaatt cacgacttgc tggtagatag ggctttgcga
    11821 ggtgattgaa aactctccaa aaccaagttg atttgtaaaa aattaaggac aatgctagta
    11881 aaatttgaat agcccgtttt ttcatattaa atcctctgtt ttctcccctt gtttcttcat
    11941 attcctaata aattttactt aaaatctcgc agcacttcct ttgcaaagat gattacctcc
    12001 tccaatatat cctaaatcat cggttcctct gggacaaaat ccatgaaccg gcatagttaa
    12061 tactggttcc atttgccaag gataataacc attaggttct ttaccatagg gatctgctcc
    12121 accttgtatc agctctaatt cttcatttgt tagatttata ttttttgtca ttaaaatcat
    12181 gatatatttc ctccgttatt gtttagatta gttgagtaat ctgctactca tacctactta
    12241 caaaaaaaac tattatatta agttggtttt ttaatttgtc tagttgcaac attgacaaac
    12301 atagtatagc atatctttgc caaatatcct cttcaaatca tgaattgtca tcaaaacatc
    12361 ttaaactata aaatcaatta gtctcaagct ttctatcaat ttcttctcca aaatatgcta
    12421 taataatagc aaaagataaa gaaggaagac ctatgattaa actactagcc ttggatatgg
    12481 acggcaccct cctcaatgaa gccaaggaaa tcccacaagc tcacattact gctattcaca
    12541 aagccattga aaaaggtgtc aaactggttc tctgtacggg tcgccccctt ttcggtgtcc
    12601 tcccctacta caaaaaactg gagcttgacc tccagaacga atatattatt gttaacaacg
    12661 gttgttcaac tcaccagacc agtgattgga gtctagttga ctggaaagaa ctgagtccag
    12721 ctgacatcga atacctctat gaccttgctg aaaagagtga tgttcagttg acactttttg
    12781 acgagtcaca ttattttgtt ctcggtggca aacccaatca agttattgaa aatgatgcta
    12841 aactagtatt ttcagacctg actgaaattt ctcttgaaga agcgactagt ggaaagttcc
    12901 ggatgttcca aggtatgttt ttaggaacaa aagaacaaac agacgatttt gagcagcgtt
    12961 ttgctgaaga gctttgccaa cgattcagtg gagttcgttc gcagcctgtc atttatgaag
    13021 caatgccgct tggaacgaca aaggctactg ctctttcacg actagctgag attttgaaga
    13081 ttgattcctc agagattatg gccatgggcg atgctaataa cgatatcgaa atgctccagt
    13141 ttgcagggct tgggattgca atgggaaatg ccagcgatta tgtcaaatct cttgcggatg
    13201 ccgttacctc aagcaacgaa gaagacggcg ttgcgcgtgc tattgagaaa tatattctat
    13261 aaaaaagaaa agcaaataga cagaagttac tgctatttgc ttttttgcta atattttaaa
    13321 atacggacta atcaatagac aagaatagcg aatcataaaa atcgatttac tagatgccat
    13381 agtatcttat agttgctaat aagaagttag tctagcagaa tgaatctctc atttctccca
    13441 cactaatctg taatttgtct atacatttct ggtcttctat ctcgaaacaa gccccagttt
    13501 agacgttcac ttgctccctt gtctaggtca taagtagcta acagaacagc ttcttcttgt
    13561 ctttcagctc gttctagaat agctcctgtt tcatccgtca taaaggagga accgtagaag
    13621 tcaagactgg aactctgtcc gccattttcc tcactaggag taacctcctc taaaccataa
    13681 cgattggctg cgatgactgg aacaatattc gctgctgcgt gcccttgcat agtacgttgc
    13741 cagtgaccac aactatctgt atccaaaatt ggctctgaac cgatagctgt aggataaaag
    13801 agcaattcag caccattcaa tgcaagacag cgggctgttt cagggaacca ttgatcccaa
    13861 cagataccga taccaatctt agcatagcga gtattccaga ccttgaaacc agtgttacca
    13921 ggcgtgaaat agaatttttc ttgataataa tggtcatctg gtatatgggt ctttcgataa
    13981 acgcccagca ctcccccatc tgcatcaatg acggcaatag agttatacaa gacattacca
    14041 tctttttcat agaaactgat tggtaaaaca acttgtagtt ccttagcaat caccttaaaa
    14101 tgctgaatgg cagtattttc cgctacagat tgggcatact ggtagtagtc atactgacgt
    14161 tcctgacaga aatagggatg ttcaaacaac tcgggcaaga gaataatttg ggctccttgc
    14221 tcagcagcct gacgtactaa acgctctgcg gtttggatat ttgttgccac atccttagcg
    14281 cattgcatct gaatggttgc aactcttaca tttctcatct ttttctccta ttctggaatt
    14341 tgttgggtga tacagtggat attgccacca cctaagagaa tatctctggc tggtattccg
    14401 acaactttac ggtctgggaa acacttgctg aggatatcta aggccacttg gtcgtttaca
    14461 tcctcaaact gtggaaccaa gacagccttg ttggcgatat aaaagtttac gtaggaagct
    14521 gctagtcgtt cacctgcgta tcgcttttct tctccttctt catagatgta gcctggcaaa
    14581 tcttcttctg tcacaacttg tcgaactgca gggataggca atttatgaat ggtgaagtga
    14641 caaccttttg catctgtttc ctgttctaag agttcgagat ctgcttttga catggcatac
    14701 tggggatcat tttcgtcatc tgtccaagcc aaaacaagct cagcaggacc aacaaaggca
    14761 gcaacattat cgacgtgttc attggtttca tcctgataaa taccataagg aagccaaata
    14821 actttttcag caccaagact ttctaataat gtgttttcaa tctcctcttt agtcaagtta
    14881 ggattgcgac caggactaag caagcaactt tcagttacga gaatagttcc ttgaccatcg
    14941 ctatggattg cgcctccttc cagtacaaaa ggtttagcat catagacagg cctttccaag
    15001 gcctcagcaa aacgactggc tacttggtca tcctcttcat aatcttgata aagaccatca
    15061 taggtgcctc cccaagcatt gaaggcccaa tccacggcta atttcttacc tttatcattg
    15121 acgagaatgg ttgggccagt atcacgcgcc caggcatcat tggtgggaat gtctaaataa
    15181 acaaccttgt ctccaagata ggattgggct tcagatagat aggcctgctc caccaaaaga
    15241 tagactcttt ccccttctgc tatggtctcg atgatctgag taaatgatct tttagcagcc
    15301 tttccttgaa aaggccatga tcctggtcga gtcggccata tcatgagggt accatgatgg
    15361 ggttcgtact ctgctggcat gtgatagcct aatttttttg gactgtccat catgataatc
    15421 tccctttaaa gtcttgatag ccaaaagctt tgagtaagct acagtctccc tgttcgtcca
    15481 tgagatagag acttggcaat ccaataccat taaaggtatt atttttgaca aaagaataaa
    15541 tggccatgtc ttgaaaataa agtctgtctc cgatttggac tggattttca aaactataat
    15601 caccaatcac atcgcccgtc agacaggtat tagaagaaag tctgtaggta tgggcttttt
    15661 cctgtgcctc aaagccattt ctcaaaggtg gacgataggg catctcaagt acatcaggca
    15721 tatggcaggt cgcagaggcg tctaaaacca agatttccat accattttct acaatatcta
    15781 atacctcagt tgctaaataa cccgcattaa gcgcaatggc ttcaccaggc tcgatataga
    15841 tttcaagatt gtaagttttt cggatacgct tgatttctga aatcagcaaa tccacatcgt
    15901 aaccttctct tgtaatatga tgaccaccac ccatattgag ccattttacc tcatgtaagt
    15961 agggaccaaa ctgttcttct actgctttca aagttgtttg taaatcatct gctccctgct
    16021 cgcaaagggt atgaaaatga agtccgtcaa ccaaatctag caaatcactc ggaatcttgt
    16081 ctatagtaac tccaaagcga gaacctggtg cacaagggtc atagagcgcg tgatctcctt
    16141 gagttgaaca ctgagggttg aggcgcaaac caacactgac accagcctct cgacaacgcg
    16201 aaccatgctt acgcaactgt ctctctgagt taaagacgat atggtctgtt atcccaagca
    16261 attcctccaa gtctgcatcc ttgaaagcag gcgcaaatac atggacttca ccaggaaatt
    16321 cttcccttgc caatttggcc tcatagagtc cactagctgt cgtacctgat agatactggc
    16381 taatcaaggg ataagttttg tagagggaat atgccttctg ggcaagcaag accttgcaac
    16441 cgacctcttc ttgtacatat tgtagaatgt ggcaattagc ttctaacttg gccaagtcaa
    16501 taacataggc tggtgttggt acttgttcta acttcattag tccaccattt gtggattttc
    16561 aaccacaacc catggcaaac catactcatt caaagcttcc atgaatggat ctggatctaa
    16621 ctcctcaagg ttatacactc cagcttgttt ccaagttccg ttcatgacta attttgtccc
    16681 aatcatggct ggaacgcctg tcgtgtatga aattgcttgt gacccgactt ctgcatagca
    16741 ctcttgatgg tcgcaaacat tgtagatgta gatagtcttt tcaacgccat ctttgacacc
    16801 tgtaaagata catccaatat tggtttttcc gactgtacgt gggccaaggc tagcaggatc
    16861 tggaagcaag gctttcaaaa attgaattgg aacaatttct tggccgttaa agttaatggt
    16921 atccgtacga aggagtccaa cattttcaag acatttcatg tgcgtcaagt aagattgacc
    16981 aaaagtcata aagaaacgaa tgcgtttgac acctggaatg ttcttggcca atgattcgat
    17041 ttcttcatgg tgaaggagat acatatcttt ttgtccaact tgagggaaat catactcacg
    17101 cttgatagac atagcttcga cttcgaccca tttcccatct tcccagtaag aacctggcgc
    17161 agaaacctca cggagattaa tttctggatt aaagttggtt gcaaatggat aaccgtggtc
    17221 accgccatta cagtctaaaa tgtcgatata atggatttca tcaaaatagt gtttgagggc
    17281 ataagctgaa aagacactag ttacacctgg gtcaaagcca gaaccaagaa gagcagtcaa
    17341 gcctgcttct ttgaatttct cttgataagc ccactgccat gagtagtcaa agtaggctgt
    17401 aaaaccaagt tccttacaac gtttttcgta gatagcacgc cactcagggt cttctgtgtc
    17461 ttctgcttcg tagttggctg tatcgatata gtgaacacct gttgccaaac aagcatccat
    17521 aatggttaaa tcttgataag gcagagctac attcaaaaca gcttctggtt tgtagctttc
    17581 aatcagggca atcacttctt caaccttgtc agcatcaagt gctgcagttt caattttagt
    17641 acttgttttg ccttctagct tcgctttcaa gtcatcgcat tttgacttgg tacggctagc
    17701 aatcataatc tctgtaaatg tttcgctatc ttgacaaatc tttgaaatag caacttgggc
    17761 aacgccccca caaccaataa ctagtaaacg actcattttt ttccttcctc ttcttctaaa
    17821 atgtcctcaa catacttggg caacataaag gatcccacgt gtaagtttgc agtgtagtat
    17881 tctgtgaaaa gctggcgttt tttccaacct tccttgtcaa aatctttgac agggtggtat
    17941 tttttcgatg caaatccaaa caaccaatag ccagctgggc tagttggaat atgggcctga
    18001 taaacccgac tgattggaaa ggattgattg accttgcggt gcatgcttcg gcaggccgac
    18061 tcatcctcgt caaagaaggg actcccatgc tggtaaatca tgatgccgtc ttccttcaga
    18121 gctcgataac tattgccgta gaattccttg gtaaagagtc cttccgtatg gccaaatgga
    18181 tctgtcgcat cgttgatgat aatatcgtaa tcatcttcgc agtttcgcaa aaagcgtagc
    18241 ccattttggt agtaaatggt aacacgagga tcatctagcc ctgcagcaaa gtctgggaaa
    18301 tactcacgac agacctcgac caacatctca tccggttcca cgatatcgat ttgttccagt
    18361 tcaggataga gtgttaatac ttgggcaaca ccgccgtcac cacccccaat aaccaatact
    18421 ttctttggat ttgggtggac agccatggga acgtgaacgg tcatttcatt gtagacgaaa
    18481 tcatccgcat ctgagaacaa gacatggcca tttaaaatca gtattttccc aaaagctggc
    18541 gtatccaaga cttcgatatc ctgccattca ctttttccag cgtaaagttg cttggctgtt
    18601 ctcagagaca atttgacatc tggagtatga acttcagaaa accataaatc catctatttc
    18661 tccttatatt atatttagcg cagttagcta ctgccaaagc ccaagtggta tacttggaat
    18721 aagccactgt ggattagttt attccctcca atcgcttgac gatttccgaa aggctctaac
    18781 cacagttttt tgtttcaatt ggtaatgagt ttgataacgc atagacgttt tatctcgtct
    18841 aaggtaacaa ggcaaaaagt tcgtggataa ccaaatagac ttgaaatact acatctggag
    18901 gtactcccat gctttatgtg ggcattgatg tcgctaaaaa taaacacgat gttacagcct
    18961 tgaatgttca aggaaaaact gttcttaaac cactcacttt ttcaaataat aaagctggtt
    19021 ttgaactctt agatctgtct cttcgacagc tcaaccaaga ctgtctcatc gctcttgaag
    19081 atactggaca ttatgccttt aatctactga acttcctgca tgaacaagga tataaagttt
    19141 acacctacaa tcctttgctc atcaagaaat ttgccaaatc attatcactt cgcaagacta
    19201 aaacggataa gaaagacgct catggcatcg ctcttaaact tctttctgac cccaatcgtg
    19261 aacaatttca acacaataat cggcaagtag aactaaaaat actgactaga catattcatc
    19321 gtctcaagaa aaaacagtct gattggaaag tacaatacac tcgttgtctt gatatcatct
    19381 ttcctgagtt ggataaaatc gttggaaagc attcagaata tacctaccaa ctcttgacac
    19441 gctaccctaa tcctcagaaa aggattgagg caggatttga taagctgata gaaattaagc
    19501 gattgaccgc ttctaattca agggcttttt gccgtgctct aaaatcatcg ccaaatcatc
    19561 aaattgaaat aagaaatagt ttagataaaa ctattgactt ttcttatagt ttgtctttct
    19621 cttaataacg ttgatatgat tgacctctgg atcttccgtc ccttggaggg agcaaccacg
    19681 ttccttggcg aattggatat agtcgacaat ttctcgtgta atgcgttctc caggagccaa
    19741 gataggaata cctggagggt aacacatgac aaattctcca cagacctgtc caacagaatc
    19801 atccaaagtt aaactttttc tttctgaata gaaggcttct tgcggagaca gcactaactc
    19861 gggctgaata tattctcctg ctatcaaatc ttttccatct cttgaataga gtctcttaat
    19921 atcagccaga gcaccaacca agcgctcgat gtcttggatg cggtcgccga tggaaatata
    19981 ggccaagata ttgccgatat caccaaactc gatctgaatg tcgtattcgt ctcgcaagag
    20041 gtcataaacc tcgatacctg ttaagccaat accctgagtg taaactgaca acttagttac
    20101 gtcaaaatcg caaaccgaaa caccgtctat taactctttt gagtaggcat agtagccacc
    20161 gatagcattg atttcacggc gggcatactc agatagctca atgactttct caaacgactc
    20221 tttaccacga agggccaagt tgcgacgtga aatatccaaa ctagccatca acaagtaaga
    20281 ggcagatgta gactgggtca ggttaattat ctgacgaacg tattcagaat tcatctgctc
    20341 cccgataagt aaaatggagc tttgggtcaa actcccacca gacttatgca tggaaactgc
    20401 tgccatatca gcccctgcat ccatagcaga aattggaagt ttatcagtga aatgcaaatg
    20461 cgctccgtgg gcttcatcta ctaaaaccat catgccagct tcatgagcca tttctgtcaa
    20521 cccctttagg tctgaacaga tgccgtagta agtaggattg ttgattagga tagccttagc
    20581 atctggatgg tcctttatgg cctgtgctac tcggtcattt tcaagaccta aagcgatacc
    20641 aatcttagga tctacactca tctcgatata gatgggaatg gcaccacata gaaccaacgc
    20701 attgatagca gatttatgga catttcgtgg cagaataatc ttatctcctg ccttgcaggt
    20761 tgccagaatc atagtctgca ccgatgaagt tgttccacca atcattagaa aggcatggct
    20821 agctccaaaa gcatctgcag ccagctcctc tgcatcacga ataatcgaaa taggatggcc
    20881 taaattatcc aagggtttca tcgaattgac atcaatgcct acacattttt ctcctaagag
    20941 ttcgacaagt tctggatttc cccgtccacg cttgtgacct ggaacatcaa agggaacaat
    21001 ccttttcttg cgtaacttca ccaaggcctc ataaattggg gcttggtttt gatctaactc
    21061 tttcaaaaca tactcctttc aatattttta ggaccttatg aaaactaagg aactaaaggt
    21121 tgactcatga aaaaagagca ggttttcacc tgctctgcaa tcttctgacg tgtttatttt
    21181 ggtttctgtt gagtatgtct gttcccgatg tttcctaact ctctagtagc aaatattacg
    21241 tactttattg atcgtttcac aagatgacgt gtgctttcac cacactaaaa atttatgaat
    21301 tagaacaagt gtcctaacat caacttataa aagtaggctt ttaaccctat caataatgtg
    21361 gcttttcaac cacgcttcgg cattcaaccc tgcctgtccg taggcatttt ttactcgggt
    21421 ttatatttgc tagaaaacat catctcattt tctaagcttt attactatat catgttttta
    21481 agaacttgta aagtattttt tgataaaaaa taaaaatata gtagattgaa actagaatag
    21541 tacacctctg cttctaaaac attgttagaa atagatttta ctgtcctgat cgatttgttc
    21601 tgttcttgtt tcattttact atatgttcgt aaatgaaagt tctataatga agttagccac
    21661 ccagccctcc taaaaattag atggttcaac tagccgatag ctcttgtagg cataagaaaa
    21721 cacctctgtg ttatacttgt tgttcaacca caaacacaag aaaggcacag agatgcaaga
    21781 caattatact acaaaagcca aacatttgac aatcgatagc cgtcgcttaa tcgaaagatg
    21841 gaaaaaagaa gggaaatcaa atagagaaat tgcctctcta cttggaaaag ctcctcaaac
    21901 tatccacact gaaatcaagc gtgggacagt ccgacaatgt cttggaaaag ggcgcttcaa
    21961 agaggtttat tctgccgact atgctcaaca gtcttatgaa aacaatcgca agcgctcggt
    22021 caagaaatca agcttgacca aggaactaaa ggaaaagatt ctccactatc ataaccaaaa
    22081 attttcgcct gaaatgatgg ttattgctaa aggggttaac gtgggaattt caaccattta
    22141 ctattggatt catcgtggaa aattggggtt aagcaagcag gatctgcttt accctagaaa
    22201 aggaaaagcg cttaagaaac aggctagcac caactttaaa cctgctggtc aatccatcga
    22261 acagcggcct gaagctatca atcttcgctt ggagaatggg cattatgaga ttgatacggt
    22321 tctacttacg agagcgaaaa actactgctt gcttgtcttg acggatcgaa agagtagaca
    22381 tcagatcatc cgattgattc caaataaaag tgctgaggtg gtcaatcagg ctctaaaact
    22441 catcttaaaa caacacaaga ttctttccat cacggcagat aatggaacgg aattcaatcg
    22501 cttgtttgat gtattttctg aggagcacat ctattatgcg cacccctatg cctcttggga
    22561 aaggggaact aatgagaatc acaacaggct cattcataga tggttaccta agggaaccaa
    22621 gaaaatgact cccaaagaag tcgcattcat cgaaaagtgg attaacaact atcctaaaaa
    22681 atgcttgaac tacaagtcac ccagagaaga cttcttgatg gctaacttga acttgaaatt
    22741 tagcgtcaga aacaaatcac gaaatcgatt caaattttgc aggtcgttta aatatcctgc
    22801 gcgcaggtgt tcttggtgct aacgatggaa ttatttccat tgctggtgtg gttatcgggg
    22861 ttgccagtgc cacgaccaat atctggatta tctttttatc aggatttgcg gctatcttag
    22921 ctggtgcctt ttcaatggct ggtggagaat atgtatccgt ttcaactcca aaagataccg
    22981 aggaagctgc cgtttcgcga gaaaaactct tgttagacca agatagggaa ctagccaaaa
    23041 aatccctcta tgctgcttat atccaaaatg gagaatgcaa aacttctgcc caactcttga
    23101 ccaataagat ctttcttaaa aatccactca aggctctggt agaggaaaaa tatgggattg
    23161 agtatgaaga atttaccaat ccttggcacg ctgccatttc tagcttcgtt gcctttttcc
    23221 ttagaagttt gcctccaatg ctgtcagtga ccatattccc aagtgaatat cgcatccctg
    23281 ctactgtcct tattgtcggt gtggcccttc ttctcactgg ttacactagt gccagacttg
    23341 gaaaggatcc gactagaaca gctatgattc ggaaccttgc tattggtctc ttgaccatgg
    23401 gagttacctt cctgctcgaa caacttttca gcatttagaa tacaagaaat acctcgattt
    23461 tgaagtcgag gtatcttttt tacatttgca caatcttgcg ataacttctt gaagtaatca
    23521 tgaaaatcag cacataggcg atgaggaaga tagcgcagat agacaaggtc acaatcaaca
    23581 tcatagtcgt atccagtaca ccaatcactt ttaaaatcag gctaagcata tggtaggcaa
    23641 aggcgagatg tatgaaggca aagagcaaag gaaggaagaa aacagttaaa acctgtttgt
    23701 tgatggtttg cttgatttgc ttttggtcca aaccgacttt ctgcaagata ataaagcgtt
    23761 cacggtcttc gtagccttca gaaatttgtt tgtagtagat gaccagaaca gttccgacca
    23821 taaagataat ggataggaaa ataccgataa agaagacacc accaaagagg gcactcatct
    23881 gagaactagc atctgctaga ttgctaccat aaacatagct accttctgtg tctaattgag
    23941 cattaaattg attgaggtag ttttcatact cctcagcgac cttgagttgt tcttcttcgc
    24001 tggcatctac attcatacca ccgtaaaact gattatagat agccgaatct gggaattgat
    24061 ccaaaaaggc ttgtaaatca ggaacaacaa ggtaattgta atcagtagtc aagatattaa
    24121 acttatttgg aacatggttg acaatgaaat ctttattaaa ttcttctttg acagaaaatt
    24181 gatgatcatt tagagttaga gctttctgtc ctttcagtcc gtcatttttg gcaaagagac
    24241 cgacctcatt tcctgataga gacagttttt gaccagtcat attttcataa tctttttggt
    24301 caaataccat gaaaactgtt tttggttgga cacggttttg tcctttttca aaaatagtta
    24361 acttggttcc ttcttgattc gcaacagcaa agtaagtgta acgaagcact tctttctctt
    24421 taatcttata acctttgtca cttgcaaact ggctcaagag tttgtccaaa tcttcttttt
    24481 caacattttg ccctgaaacc ccaaaatcat gaggatttag aactttttta aaggattctg
    24541 cggaattgaa aatgcttgtc gctgctgaca tggttaccaa aaccattgtt gacaaaatag
    24601 cgatagttgc tagtccaact gcatttttct tcatacggaa aatcaagtta gaaacagata
    24661 tgagattatt aggttggtaa tagtatttct tatttttctt taagatttga aggaaaacgg
    24721 taatccctgc attgaacaaa agataagtac caaagataac cagtaaaaca gctaggaaga
    24781 aagttgttag ggctgtaaga ggatcggtta ccgtaagggc aagataatag ccaatcccta
    24841 aacttatgga accaagaatc gtttggagag gaaggaagcg accttttttc tctccgctag
    24901 ctttctcacg tgagagctgg agggcattca tacgggcgat tcgaagggca ttcaggaaca
    24961 tgaggcctag gaaaatcaaa ccgaagacaa gaagtactgt aatgacaact ttcatctgga
    25021 aggtagcgac cagttccacc ttcaatttca tcagtttgag taggaaagcg aaaatcaact
    25081 tgtcaaacaa ggctccaata ccgattcctg ctccaacagt tagaatccca aatagcacta
    25141 actccttaaa ggtcatactg attagatggc gcttctccaa gcctaacatg ccatatatac
    25201 ccagttcctt ggaacggttt ttcatgacaa aactattggc atagaggacg ataatggctg
    25261 acgcaagggt aacgacaaac ataccaaatc caagtgttgc ttgaatggtg gttcctccac
    25321 ggatttccgc aatctttgga ttgaaggtta gggagtaaaa gagataggtg atggtgactg
    25381 ccaagagaac agccagtgca aagggatagt agagtttgcg gtttttaatc aagttcgata
    25441 ccgctaactt attggttaat cgaaacatac taattcacct cgcttgccat gacagtcaag
    25501 gtatcagaga tttcttggaa catctgacgc tctgtcttct ctccacggta gatttggttg
    25561 taaagaatgc cgtctttgat aaagagaaca cgcttggccc tgctagcagc tgctgttgag
    25621 tgggttacca tgaggatggt ttgcccacgc tcattgattt cattaaagac atcaagtaag
    25681 gctgcagatg acttggaatc aagggctcct gttggctcgt ccgcaaggag aatttcaggt
    25741 tctgtgatga tggcgcgggc tactgctaca cgctgtttct gaccaccaga aatctcgtaa
    25801 gggtacttct cttgcaattg gttaataccc agattctcag ctgtcaccac caatttcttc
    25861 atcatctccg ttataggtct tcttgacaag acaagcggaa gcaagatatt gtccttaaca
    25921 gacagagtat ctagcaagtt aaagtcttgg aagacaaatc ctagcttttc acgccggaaa
    25981 ctagaagcct gtgaattttt aatagttgcg gtgtcagttc cattcaagta aacctgccca
    26041 cgagttggtt tatccaacat agctagaata ttgagaagag ttgatttacc agaaccagac
    26101 tcacccatga tggcaacgta gtcacccttt tctacggtaa agtgaatatc cttgagggct
    26161 tctacttggt tgccctgaaa acgtgtttta taaatttttt gaacgtgttt tacatctaaa
    26221 agtgtcatga gaatctcctt tcttaatatc ccatcaaatg aaatgtagct tgcatcatag
    26281 tgcatcccag ctgaacgcgc tcaatttctt tgtgacttgg ttttctagtt ttcttatgta
    26341 agatttgttt catgatgtta ctcctttgtt ctttatgagt ctagtttaca tcaaaaaaag
    26401 accgcttgcc ataacctaac cttacaaaag agactttaat cttacatttt tgtaagattg
    26461 ggtgaaatat aagcaaaatt acttagaaaa tgatttgatt tcctattttt aaagttgtaa
    26521 aatataagtg tgaaaaggct tactttgaaa aggaggaagc gggaactaac attttagtaa
    26581 gcttacctag ataacatacc attcaattta acacttgcaa gtcgttcaac ctataaggaa
    26641 agaaggtgcg ctttaatgaa acacaaagaa catatactca tcggacttct ctatctgctc
    26701 tctccattca tcggtcagct cttagttgaa cacacacact ttatcagtac agaatttacc
    26761 ggcactgctt atgttatttg ctggttatca gttgttatca gcatccacca tttttccaaa
    26821 aatgtattgt ctcaacagca aaaatagatt aaaaaactgc gtttaaatta ctgcttttat
    26881 agtaaaatga aataagaaca ggacaaatcg atcaggacag tcaaatcgat ttctaacaat
    26941 tttttagaag tataggtgtc ctattctagt tccaatccac tatagtttgt ggatgaagct
    27001 ggcgaaatat tattaatact attcaaaaat caaagtgcaa actatatcaa cttcacattg
    27061 ccgactaaat tacagcctgc ggctggcttc ctagtttgca ctttgatttt tattgagtat
    27121 aaaagtaaaa aagctttaaa tcaagcagtt tagactattg atttaaagct ttttgcgtat
    27181 tccaaatctt ttgtaaaacc tcaccaaaac cagccttcat tatcgtcaat ggcttgagct
    27241 tctttgcgtt tacgtgggcc tgttccgtac atttctgctt cgatttcttc cttggttggc
    27301 atgatagaag ctaggatgat atagataagc accccaagtc caaagtttgc gactgtaaaa
    27361 atggcaaaca gaaaacgtac aagggtaaca tcaaagttcc acttgtctga cagacctgct
    27421 aaaactcctg aaatcatgcg atttcgtctc attttataaa acttactgtt catgatattt
    27481 cctctttctg ctaggttaga ctagtatatc acggataggc tcggctttca tcagtcctca
    27541 ggctgattta ctaatagcaa ctttcctcga caaagtccac agcgatagcg tttggtatca
    27601 atcctacgct tacgctgata agtttgctgg caggattggc aacgatagag cttgattggc
    27661 ttggagttac tattgggcaa ggatggtaca aaccgtaatc catccactgc tttcaacagt
    27721 tccttaaaat cccgatcctt gtgttgatag cctttccctt gaaaatagag gtgataatga
    27781 cagagttcat gtcggacaat tttcctaaaa acgtccaacc ccagttcctg ataaaccttg
    27841 ggattaaaat ccaaatgccc atctttgggg aaaaatcgcc cacctgtcga acgtagacgc
    27901 ctattccact ggacatgatg gataaaaggt ctgccgaagt cttctagtga aacctgcttg
    27961 acgtaatcag tcagtttcat ttggagctag gagagacaga ttaacttttt cacgttcagt
    28021 atcaattttc ttaacccaaa cgctcaccaa atctccaact gccaccactt gactagggtg
    28081 tttgataaac ttgcgactca tatgggaaat atggatgaga ccgtcctcat gaattccgat
    28141 atcaacaaaa gcaccgaaat caacaacgtt acgcaccact ccttctagct tttgtccaac
    28201 cactaagtcc ttgatatcta ggacatcttg acggagaact ggagcatcaa aggaatcacg
    28261 gaaatctcga cctggtttga gaagatctgc aatgatatct ttaagagttt ctggaccaag
    28321 gtctaactct tgcgccattt ccttgactga aagcgacttg agtttgcttt gggcctcttc
    28381 attcagatcc ttaatgtcca agcatttgaa gagttcctta acggcagcgt aattttctgg
    28441 gtgaactcct gtattatcaa ggatattgct actttcaggg atacggagga aaccagcagc
    28501 ctgttcaaaa gccttggccc ccaaacgagg aactttcttg atttgggagc gtgaagtgat
    28561 ttttccctct tcctcacggt atttgacgat attttcagaa atggttttat tgagtccagc
    28621 tacgtgtgaa agaagagctg ggctagctgt attgacattg acaccaactt ggttaaccac
    28681 tgtatcgaca acaaagtcca gactctcaga gagtttcttc tgactgacat cgtgttggta
    28741 ttgaccgaca ccaattgact taggatcgat tttgaccaat tccgcaagag gatcttgcaa
    28801 acgacgggcg atagaaatgg cagagcgttt ttcaacggtc aagtctggaa actcctgacg
    28861 agcaagttcg ctggcagaat agacagaagc accactttca ttaacgataa catagctgac
    28921 ttcagggaaa tctttcagaa cttccgctac aaaagcttca ctttcacgac tggccgttcc
    28981 atttccaatg gcaataatct ctacaccgta ttgaccaatt aaatctgcta aatctttctt
    29041 ggcttcttcg atttgacgag ctgatgctgg tttaacagga taaataacct gagttgtcag
    29101 catttttcct gttgcatcca cgacagctag cttggcacct gtacgaaagg ctgggtcaaa
    29161 cccaagaacc acgcgccctt tcagtggagc aaccaagagg agattgcgca gattgtcaga
    29221 aaaaagttgg atagctccct cttcagcttt ctcagttaat tctgtccgaa tacgacgctc
    29281 aatagcaggc aagacctttt tcttaacgga ttgctgaaca acttcatcaa tataagcatt
    29341 tttcaccttg aaacgagtag caaagaaggc aagaatacgg tccgtcgcat gttcaaaacc
    29401 gatcttcaag acaccaagtt tctccccacg attgagagcc aaggtacgat agccttgcat
    29461 agttccaact gtctctgaaa aatcataata aatctgaaaa acctgctttt catcaagact
    29521 ttcatccttg gcttgagaag tgagtttaga gtgtctcagc acttcctgat aagtcataga
    29581 acgcaaggtc acatcttccg ataaagcttc gaccaaaata tcaactgcac cggtcaaggc
    29641 ttccttgcca gtcgcaaatc cttcacagac gaacttttca gcttctttct ctaagtcaac
    29701 tatattctgc aaaatcaagc gagcaagagg aaagagtcca gcttcacggg caatggttgc
    29761 cttggtacga cgcttttcct tataaggaag atagagttct tcaacgtctg ctaatttttc
    29821 ggcaactaag atagcttctt ccaattcctt ggtcaactta ccttgttctt gaatcttagc
    29881 taagacagct tccttacggt cattgagatt tgtcagactt ttatccaaat caataatagc
    29941 cttaatcgcc acctcatcca gactaccagt catgtccttg cgataacgcg cgataaaggg
    30001 aatagtcgcc ccttcagctg tcaaacttag aacggtatca atttgcttta acgtcactcc
    30061 caaatcctga gagatttttt catatttttt atccataaat ctattatacc acaagctgga
    30121 aggctttctc caatcctttt catactcaat aaaaatcaaa aagcaaacta ggaagctagc
    30181 cgtaggctgc tcaaaatact gttttgaggt tgtggataga actgacgaag tcagtaaccg
    30241 tactacggta aggcgaagct gacgtggttt gaagagattt tcgaagagta tcaactatcg
    30301 aaaacaaatt aaaaaactcc cttttccgca aaataatagt ataatagagg tagaatttag
    30361 aatcgaggta cacctatggc tgtcaaattt acaaaacgag acgacttgga caagatgttt
    30421 gaagagtttg ctaaactccc tgatttgaaa caagtcactt tccctgatga caaagagaaa
    30481 aaagccaaag cagaaaagaa aaactagatg actgcttttc aacaactccc atctagtgta
    30541 cttcaaactg gagccatttt tctctccatt atcattgaag cccttccctt cgttctgata
    30601 ggaagtattg tctcagggct gattgaagtt tatatcacac ctgacaaggt ttatcatttt
    30661 ctccctcgaa atcgttgggg gagaatcttt tttgggacct ttgtcggtat acttttccct
    30721 tcctgtgaat gtggaatcgt ccccatcatc aatcgttttc tggaaaaaaa ggttccaagt
    30781 tacacggccg ttccttttct tgtgacagca cctgttatca atcccattgt tctttttgcg
    30841 acctattctg cctttggcaa ctccttccat gtcgccctat tacgagctct gggttccatc
    30901 gttgtggctg taatactagg aatttttcta ggatttttct ggcaagaacc gattcaaaaa
    30961 gaaaatcgtc tagcctgtca tgagcatgat ttttctcact taagtcctgc aaaaaaagtt
    31021 tttcaggtct ttgtgcaagc cattgatgag ttttttgata cgggacgtta tttggtattt
    31081 ggctgtctct ttgcctctgt tatacaggtt tatgttccga ctcggattct gacctctatc
    31141 agtgcgactc ctctttttgc catcctgctc ttgatgattt tggcctttct tctttcgctc
    31201 tgtagtgagg cggatgcctt tatcggggct tctctactct cgagtttcgg tttggctcca
    31261 gttctggctt ttctcgtcat tggcccaatg ctggatatca aaaatattct tatgatgaaa
    31321 aattacttga aagcacgatt tattagtcac ttcattacga ttgtgactct ggtcgtttta
    31381 gcctattctc tcttgattgg agtcatccta tgattcgatt tttagtttta gctggctatt
    31441 ttgaactgac tatttacctc catctgtcgg gcaaactaaa ccagtacatc aacatgcact
    31501 attcctatct ggcctatatc tccatggtgc tttcttttat cttggctatc gttcaattgt
    31561 atatctggat gaagcaagtc aaaacccaca gtcatctgaa cagccgatta gccaagatga
    31621 cgagtatttc tcttctggct attccacttg tcatcggctt aactttccca actgttagct
    31681 tggattctca gactgtttct gctaaaggtt atcatttccc cctatcggaa ggaacggatc
    31741 tagccattca gacaagcgaa gggacgacaa gccaatattt gaaaccagat accagttctt
    31801 atttttcaaa atcagcctat gaaaaggaaa tgcgaacggc ggcggataaa tacttatccc
    31861 aagatagtat tcagatcact aatgaaaact atatggaagt catggaggct atctacgact
    31921 atccagatga gtttgagggc aagacaatcc agtttacagg ctttgtctat aacgacccca
    31981 gtcatgccaa tagtcaattt ctgttccgat tcggcattat ccactgtatc gcagattctg
    32041 gtgtctatgg attgctgacc aagggcaata cccggcagta tgaaaacaac acttggataa
    32101 cagccaaagg aaaactggtc aatcactacc ataaagaact caaacaaaac cttccaacct
    32161 tggaaatcga cagctttacc aaagtcgata aaccagaaaa tccctatgta tatagagctt
    32221 tttaagaaaa tcaagataaa aacgaacaag ttctcttctg aataacagaa aaagagcctg
    32281 ttcgtttttt gttatatgaa aattagtgac ttgtagattt tcatcttata ccatttccag
    32341 caatacaagt agctcataga aaataagcga gccactcatt cattagacta gcgatttctt
    32401 taggtgcttg agtataaagc tcatggccaa agttttctaa aaaaatagta tcaaaatagt
    32461 ctggcaattc ttttagggct tcctctctcc atgtagcttc attaggatag cgaggactaa
    32521 taaacaaggt atctcccact tctctcttaa aagcttgtat ttttctccgt aggcggagta
    32581 tcgcttctat attttcataa tttatagcca actcatatct attatactca acattccagt
    32641 gataggactg tcttacagct ttctccatat tttctgacca atgctttgct tcagattttt
    32701 ctttagaagt aagaacatct aagtccaaaa caatttgaga tttgatataa tttttagttt
    32761 cctctaactc tgtatccaaa ggtaaaatct tatctaaatc tagatagcca ccatccaaaa
    32821 gaatcagttt ctttacttct tcaaattccg atgcgagata acgagctaaa tctcctccaa
    32881 gagaatgacc tatcagacag ataaattctt cctctacaat ctcattttta aaccatgatt
    32941 tcaattctgt ttcatctcga agatgctttt catatggatt tagaaaatag acctgcgaat
    33001 ctagttcttg aagaaaatcc ttgctatgat aggcattgct tcccaaaccg ccaataaaat
    33061 attttttcat tctctactta atactatgct tattcatctt ttgttcaaag atagttgtga
    33121 taatctgacg caattcttcg cattcttttt ctggaatctc accacttgtt tgagctacag
    33181 cgtagagttc agggtgttca attgaaatgc gtttaatcgt acgtgttgta gcatgttttc
    33241 tgacaaaaaa cgggattcgc ttaatcaagt cttgtggtac tagcgccaga atcttctcag
    33301 tagtttcttt gtcactaata ttagacattg taagcctttt cttaatcatt tcctgttctt
    33361 tttctgtaaa atcttttaat tccattcgat tagtcctcct attttctcta agttaaatta
    33421 tgtactaata cagatgaaat tacaaagaat aaactttaag aaatcttctc actgataaga
    33481 ttttagcatt agacttcctg cgaaacaaaa tatggtatag tagttctatg aattatgaag
    33541 caagtaaaca actaactgat gcacgattta aatgtcttgt tggtgttcag cgcaccactt
    33601 ttgaagagat attagctgta ttaaaaactg cttatcaact taaacacaca aaagggggac
    33661 gaaaacctaa attaagccta gaagaccttc ttatggccac tcttcaatat gtgcgagaat
    33721 accgcactta tgaacaaatt gcggctgttt ttggtatcca cgaaagcaac ttactccgtc
    33781 ggagccaatg ggttgaagta actcttgttc aaagtagttt tacgatttca agaactcctc
    33841 tcagttctga ggacacggta atgattgata gacattccca tcaatatcgt atctttggac
    33901 atagccaata aatgtttcat ttttgcgtgg tttctggcta ttaacgattg aaataaccca
    33961 ccaactcatc aaaaatagaa ataaaaatcc taagattact gtcatatcat aacactatta
    34021 aagtttaacc cacttatcat tatccatgat aaaaggctta gccagtccct cgcctgtata
    34081 atccgcatac ttggtgccca aatacttgta gcaatcttcc ttactagcaa atttaatcgc
    34141 ttggtagggc tcttcgaaag tcaatttctc tacaaataag aaaccgtcat cagcaggtac
    34201 taagacccca acgtggccta caaacagata ctcgccatcc agattgtcgt gcaagactac
    34261 agacagcatt cgagcttttt cattgaattg aaattgtgag aagaatgttt ccatcttttc
    34321 agcgtgaacc ttgacatctg tagttgcctc agttggaact ctcgaaaata gaatatcaaa
    34381 ctcttcctta tcttgtgaat caaagacctt tcctttatca atcgcatcat tatctaggaa
    34441 aagcaactgg tcattctttt caagctttgg aatggtgact gaatttttca aaagacaata
    34501 actattgata cggcagttgg tcccaacaaa atcgcccttc ttttgattcc agagatgact
    34561 gattttctca acatcgtatt cggtgtgagt aaaggaagtg aaatctcctg ataagccagt
    34621 tgagccgaca atggtattat agtcattaac gagattaaaa aatgcatcaa cactatttgg
    34681 atccaagtga gctgataaga gagatttgac ctcttctgta cttacctggt tgtttaggtt
    34741 ggtgtatgaa gctttccatg gaactttcgc tgaactgctt tgcctttgat tcgtcccctc
    34801 agaagtagca tgttgttgtt gacaagcagc caagcctaaa aacaaagctg aacagattcc
    34861 taatgtggct aattttcttg atttcttcat ttctttctcc taaatgtctt ggattaaagt
    34921 ttctttaact actgctttac agatattgat tactttctca tttaatgtgt tcatcgtctt
    34981 tccctccgat ttgttttttt gttatttcct taagcttgat ttaattatag taggcgtttc
    35041 gtgctctgtc aacaaatttt ttacgaaaac acaacaaagg ttttcatttt cgtggatttc
    35101 gtgtgatgct ttacttacaa aaatagaaag cgatttcatt cgtagataaa gaagagattg
    35161 cgattgttag aggtgaaaaa cataatagat tgaaataaga tatgaacaac tctattagga
    35221 aaggcaaatt aattcctaga aatgttttag cagctacagc gtactattcc aaactcaatc
    35281 aactataaaa caatatagac tagtaatggt tggactcaat aagaattgga tactaagata
    35341 ggtataagta aaaacaccat tagaaattat gaaaaaaggg ttaggtctac taagaaaaat
    35401 actatatttg acttagttaa agtattcagt agcttgattg atgccctctt ttctccagtt
    35461 caaaaggatt ctcctagcga tatccaatct atctacgacc aacgggcacc acccagacaa
    35521 ggtaaagttc tgacctatgc cgagaggcaa ctgtatgatc agaaaaacga agtatcgtaa
    35581 aaatcatcag actggacgac tacagacaga ctacttactc tcgtgttagg tagtttatct
    35641 ctgcaggtag ccgctcgatg caggacaacg atttagatat ggagtttcca ttatcccaga
    35701 cgactacgac ccaatcgctt atgtcgtcag caagtccatg gagccaaaga taagaaacag
    35761 cgactaccta tttatcaaga ataccccaca gattgactat aataccatcg gcatcttcca
    35821 agtagacggc gctaactatg tcaagaaact ccgccaaggc taccttaaaa aaactcaatc
    35881 ccaaataccc cgacatccac ctagacgaaa gcaaccatat tcgtaccatc gggaaagttg
    35941 tcaggctata tagggagaat taaaaaaggg ggagaatggc gatataaaac aaacagattt
    36001 taaaagattc gaaaaacttt gaaaaagttc attctacatt tcaaaaaaag aataagtgac
    36061 ctaaaataga ttagattttg ttaaacaata gtgatatgta aatttagaga aaggcattta
    36121 acatcacttt gcaaaaggca aatactaaac tagagaaacc gataaaccag ccccttagtt
    36181 cccatatttt caggcacaca ctccttagta cactagctga gaagaatgct ataatggtta
    36241 gagtggaaca caaaaacgct aaaacagtaa ataacatcta cactcacgta agtaaaagaa
    36301 tggagtaagc tactttggag gttttaaata ccatatcatt aaatcgaaaa tatatcagat
    36361 cgaatctaga caagtacatt acgattgcta aaacatttgt agaaattcat ttgactttct
    36421 aatcaatttg tgcatatctt atttcatact caatgaaaat caaagagcaa actaggaagc
    36481 tagccgcaag ctgtacttga gtacggtaag gcgacgctga cgtggtttga atttgatttt
    36541 cgaagagtat caatccatta tgaaagtttt gccccctgga caaaaaaaga gcccatcata
    36601 caggctagaa agctagatat gataggtttt tatatttttc ttaacgtact gtgcggatac
    36661 gcattgtgtt tgtgcgaaca gcttctccta ctggcacacc agcaacgata acgatatcat
    36721 cgcctgactc aacgagacct gcttctaccg ctttacgttc agcgatttcg aacatatcgt
    36781 cagttgaaga tggagcatct gtcaacattg ggataacacc ccagttcaac atcaagccac
    36841 gttctgtcaa ttcgtcaaat gtcaatgcta agatgtcagc atttggacgg tatttagaaa
    36901 tcaaacgtgc agtatgacct gtcttagtaa gagttacaac caatttgata tccattgagc
    36961 tagtagcatc tttaacagca gaagccatta cttctgtctt agagttacgc tcaaatgaat
    37021 ctgaatcaag acgtccgtat tcattaagaa gagcttgagc gttcttgtcg attgtagcca
    37081 ttgtagttac tgactcgagt gggtatttac cgtttgcaga ctcgcctgac agcattgtag
    37141 cgtcagttcc gtcgataaca gcgttgaata cgtctgatac ttctgaacga gttgcacgtg
    37201 gtttttcagt cattgtttca agcatgtttg ttgcagtgat aacaacttta cctgcagcat
    37261 tgactttctt gataatcatt ttttgataaa ctggaaccat ttcgaacggt acttcgatac
    37321 ccatatcacc acgagcaatc ataataccat cagctgcttc gataatttcg tctaagttat
    37381 cgataccttg ttggttttcg attttagcga acaattgaac atgtccgttt ccagtttctt
    37441 cacagattgc acgaacttcg ttcacatctt ttgcagtacg tacgaatgaa attgcgatga
    37501 agttgatacc ttgttcaaga ccgaaacgga tatcgtcgtt atcgcgttca gcaagagctg
    37561 ggaaaggaat tttagtgtta gggatgttca cacctttttg tttagcgatg ataccatcgt
    37621 tttcaacttc aacttcaaat tcacgagttg catcatcttt agcaaccaca cgaagaccaa
    37681 gtttaccatc gtcaaccaaa acttgacgac caacttcaac atcatcatag atatcaagag
    37741 caccagcaac gttcaacgca atcacttcac gagttgattt gattccttgt ttagttgcaa
    37801 cacgaatttt ttcaccagtt ttatatgaat attctttagc ttcaccttcg aacaattctg
    37861 tacggatttc aggtcctttt gtatcaagaa ggaaaccaac ttttttacct gcaatttttt
    37921 ccgcaagttt aacagttgcc atacgctcac cttgttcttg gtggtcgccg tgtgagaagt
    37981 tgaatcggaa tgtgttagca ccagcttcaa tcaatttagc aatgttttta gctgaagctt
    38041 caacatcaag tttttcaccc cagtatccat cctcaccgaa ttttttacca ccacggattt
    38101 ctaccgcagg acccaaagtt gcaacgatct ttacacgttt gttcatgatt tttgtgactc
    38161 ctttatatat atgacctttt tggtcttatt aactaaattg taaattatga caagctctta
    38221 ttcaagctag atagctcaat atcggctttg tgcgggttgt taaccacaat cttaccttct
    38281 gcagtaaggc taaacaatgc cccttcttct gcagtaccaa gaattggatt ttcaaccatt
    38341 ttttcgttac gaataccaac cgcaacacca ccgatacctt ctttaagaag tttaacagca
    38401 tgtgcaccca tacgtgacgc caaaacacgg tcacgcgcag ttggagaacc accacgttga
    38461 atatgtccaa gttctgttac acgaaggtcg cttgtatctc cagcttcttt aagtttttga
    38521 ccaaattcag ccgctgacat cacaccttca gctaagacga tgatattgtg ttttttacca
    38581 cattcataac cagctttgat gcttgctacg atatcttcca tcttgaagcc tgcttcaggg
    38641 atgatgattt catcagcacc agttgcaata ccagcccaaa gagcgatatc accagcgtta
    38701 cgtcccataa cttcgattac aaaagtacga cggtgacttg atgatgtatc acgaatctta
    38761 tcgatagcgt ccatggcagt agtaaccgct gtgtcaaaac cgattgtaaa gtcagtacca
    38821 acgatatcgt tatcgattgt acctggaaga ccaatagctg ggaagccatg ttcagtcaaa
    38881 cgcatagcgc cgtggtaaga tccgtcacca ccgataacaa ctacaccttc aattccgtgt
    38941 tttttcaatt gctcaatccc tttaagttgc ccttcaagtt gagcgaactc tggataacga
    39001 gctgagtgaa gaaaagtacc accacgagaa atgatgtccc ctactgaagc tgcatctagg
    39061 ggatgaattt caccggcaac cataccagca tatccgtcat agataccaaa cacttccatt
    39121 ccttctgaaa ttgcttgacg aacaactgca cggatggcag cgttcatacc aggtgcgtct
    39181 ccgccactag tcaaaacagc aatacgtttc atattggttt atgctccttt ttcttttaac
    39241 attcttcttg attatatcac atttgatttt aaaattcttc tattttccgt atttttagcg
    39301 ataaatcgtt ttcataacga tttcattcaa tttctcctct aattcattgg atttagctac
    39361 aaaatgatgg ggagaaacga tggttttctg ttcctcttca taccggatga tgactgggat
    39421 tgggccttta aattgttcta aaatgcgtga aatttcttga tccgattcat gatttttcac
    39481 ctgtatccaa aagcgttcag caactgcttc tcttatttct tgtgcaatca tttgcagacg
    39541 gccatcacgt gattgtattt ttccttttac atagtagaag gctccctctt ttatttcctg
    39601 tccaacctga cgatataagt ctgaaaagag agtgacatcc aattttttct tactatcatc
    39661 tgcctgtaag aaggccatat tttcaccctt tttggtacga atcactttta ttttctgaac
    39721 ttcaaccaaa ataatagcat agctattttc tgacaaattt ccgattgggg taatcgggta
    39781 aatagcctta cttgcaatag cttgtagtgg atgtttgctg acacctatcc ctaaaagctc
    39841 ttgttccata taaaattttt cttgttccgt ccaatcttcc gattcctgcc aactataaat
    39901 agcatctcca aacaaacttc ccaactcttt cacaaattca aatagattag ctaagttatt
    39961 aaatactttt tgacgatttt tttcaaatga atcgaaaaga ccaactttta ccaaaggttc
    40021 tagcagagga agtttcagat aattctcagg taatttagct ataaaatctt caatgttaga
    40081 ataaggtcta tgttcaataa tccaaagcgc caagtccttg ctgagcccct taatcgattt
    40141 caaacctata tagatagact tgttggcaat tttatcgtga tagggaatag tattgatgga
    40201 tagagaggct acttcaaaac ctgcttcaag tgcatctatt aagtaatcac tgttggaata
    40261 atttaacatg acctgataaa aaatggctgg ataatgcgtt ttgaaataag ccaactggaa
    40321 ggccaaggct gagtaggcgt aggcatgaga tctattaaat ccataacctg caaacttctc
    40381 cataacatca aaaacctgct ctgctttttc cgcagtatgg cctgcttcta tggagccttg
    40441 aataaaggaa gccctcatct catgcatagc agaggcatcc tttttaccca tagctcgacg
    40501 caaaatatcg gccttcccaa gactaaatcc agcaaatcgc tgagcaacct gcataacctg
    40561 ctcctgatag agcataatgc cataagttgg agccaaaata tcctccagag ctgaatctag
    40621 aacagtcact tcttcctgcc catgcttcct tgccacaaaa ttattgatgt agtcacttgc
    40681 acctggtcga tttagagaag tagttgctac gacatcttca aaacagactg gttgaacacg
    40741 tttgagcaag cgaatggcac caggttgctc aaattgaaag ataccttttg tatttccaga
    40801 ggcaaataaa tctaacgttt ctttgtcttc caaatctatt tcttcaattt taaggtgaat
    40861 accttctgtt tcagcaagca actcttgcat cttctggaca aaggtcaaat ttcgtagtcc
    40921 cagaaagtcc atcttcaaaa gtccgctagc ctcaactcca tgagcatcat actgagtcag
    40981 tggaatttca tcaccatact ttagaggaat gtagttggtt aaatcttggt cactaattac
    41041 aacaccagcc gcatggacag aggtttgcct tggatagccc tctatcttgc aagcaatctc
    41101 aaaagctttt tggtattcta acttactatt gatttgctga cgaaactgga gattgccctc
    41161 ataggccgac ttaagattgt cacgaaaact gattttctta gtaattgcag ataattcata
    41221 ctctggcaca ccaaagcgtt tcaagacatc tcgaagagct tgcttggctc caaaggttga
    41281 aaaagtaacg atttgtgccg catgtttact accatattta ttaccaacat atctgataaa
    41341 atctggacga taaatatctg ggatatcaat atcaatatca ggcatggtat agcgttcacg
    41401 attaagaaag cgttcaaaaa tcagattttt ctctactggg tcaatccccg tgatgtctaa
    41461 ggcataagaa accaaactgc ctactgcaga accccttccc attcccatat aatagccatt
    41521 cgatcgtcca aaacgcaaca aatcccaaac aaccaagaaa taatcatcaa agcccatatc
    41581 atgaataaca gacaattctt ggtctagtct atcttgatat tctttactag tcaacccctt
    41641 ctgaacaaac cccagttcag cacgctctct caactcctct actgctggtc tagctggatt
    41701 aaaacgaggc agtttcagac taatatccaa gtcgtaagaa atgcctgaaa taagcttttc
    41761 taaattgtcc aaagcttgcg gaaaacgctc ttggaatagt ttctctaaag aacttgctga
    41821 tataaagaca tcttgtctcg aacgcaaggg aacttctctg agcggtagat tttctttaat
    41881 cgctgttaaa acttgaagaa cttctctatc cctgctttca aaagcgttga cccgataaag
    41941 aggtaagata ggatgatgaa attcgcttgc tagtgtttct ggataaaccc ctatatagta
    42001 atcacagcct agttctaacg actcaactct atcaaaataa ggcacaatga ccgcgatatc
    42061 ctccaggtac tgggacagga ctgaccaagt tttctccccc tgcatcttgg ctgtcgaaag
    42121 cttcatcaac tgctgatagc ccacactaga tagagctaaa aagcgcaaat tcacttcctg
    42181 atcatctaca aacactgtca tttcaagccc tagcaaagga tgaatgccgt atttttttgt
    42241 aatctctaga aagtcgaaag cgccataaag attgtcaata tccatcatag ccaaatgagt
    42301 gtagccgtat tctttagctg ctctcacata cttttcgatc gaaatgacgc tttccataaa
    42361 actatagact gtttttgtat ctagttgtgc gatcaattta cacttctcct ctatccttct
    42421 cactatatta taccattttc acctataaat ggcttctctt gagaaaaatt tcatcagctt
    42481 actaatttat tttctattca tttgtatttt tgtcatcatt t
//
LOCUS       DBERMR010000011        42082 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.011, whole genome shotgun sequence.
ACCESSION   DBERMR010000011 DBERMR010000000
VERSION     DBERMR010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 42082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42082
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.011"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            12..392
                     /locus_tag="ACL8A1_000661"
     CDS             12..392
                     /locus_tag="ACL8A1_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201471.1"
                     /translation="MKLKTLALISGIVGLVGGILLLIGPFVLLGTTVNTAATTLNGGA
                     TAGAFSGVALLLNALKIENLVLGIIAIVYYKGDKRVGAAPSVLMIVSGGVSLIPFLGW
                     VGGILAIIGGSLFLATLKKFKSEE"
     gene            927..1619
                     /locus_tag="ACL8A1_000662"
     CDS             927..1619
                     /locus_tag="ACL8A1_000662"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HGS1201472.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            1672..4083
                     /locus_tag="ACL8A1_000663"
     CDS             1672..4083
                     /locus_tag="ACL8A1_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HGS1201473.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGLI
                     GSERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALATTSYVYKGTRFE
                     REAFASFPDDLLVQCFTKEGLETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLETVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDENLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAARASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKISTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            4333..5034
                     /locus_tag="ACL8A1_000664"
     CDS             4333..5034
                     /locus_tag="ACL8A1_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1201474.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPAQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            5036..7744
                     /locus_tag="ACL8A1_000665"
     CDS             5036..7744
                     /locus_tag="ACL8A1_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HGS1201475.1"
                     /translation="MIKRKTYWKDLIQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMETTANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEELEQILSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEAKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGSILGHYLL
                     ANVISSVITKGMVVGETQIQFYWTYSLLALVLSLLASVLPAYLVAWRELHDEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKAL
                     DKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVATVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(7840..8331)
                     /gene="tpx"
                     /locus_tag="ACL8A1_000666"
     CDS             complement(7840..8331)
                     /gene="tpx"
                     /locus_tag="ACL8A1_000666"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HGS1201476.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(8454..9383)
                     /gene="psaA"
                     /locus_tag="ACL8A1_000667"
     CDS             complement(8454..9383)
                     /gene="psaA"
                     /locus_tag="ACL8A1_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HGS1201477.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(9415..10263)
                     /locus_tag="ACL8A1_000668"
     CDS             complement(9415..10263)
                     /locus_tag="ACL8A1_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HGS1201478.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(10260..10982)
                     /locus_tag="ACL8A1_000669"
     CDS             complement(10260..10982)
                     /locus_tag="ACL8A1_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HGS1201479.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
     gene            11273..13165
                     /gene="pepO"
                     /locus_tag="ACL8A1_000670"
     CDS             11273..13165
                     /gene="pepO"
                     /locus_tag="ACL8A1_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HGS1201480.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
     gene            complement(13288..13917)
                     /locus_tag="ACL8A1_000671"
     CDS             complement(13288..13917)
                     /locus_tag="ACL8A1_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGS1201481.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            14911..17133
                     /locus_tag="ACL8A1_000672"
     CDS             14911..17133
                     /locus_tag="ACL8A1_000672"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HGS1201482.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRMIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            17163..17606
                     /gene="dtd"
                     /locus_tag="ACL8A1_000673"
     CDS             17163..17606
                     /gene="dtd"
                     /locus_tag="ACL8A1_000673"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HGS1201483.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(18078..18491)
                     /locus_tag="ACL8A1_000674"
     CDS             complement(18078..18491)
                     /locus_tag="ACL8A1_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201484.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFIIRLETSFEWLLLSGLIFFLA
                     QGLLLFLLIVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLRENYPDFYDKHISLWECL"
     gene            complement(18622..19329)
                     /locus_tag="ACL8A1_000675"
     CDS             complement(18622..19329)
                     /locus_tag="ACL8A1_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0034257 - nicotinamide riboside
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter family
                     protein"
                     /protein_id="HGS1201485.1"
                     /translation="MKKSLKIFATSKWFDLFGVALVVGIAIASGYLNSRLDKFVDWGP
                     WTALVPFGLISVTNVGISMLSTRFTGKLSKWGNYFSIVNTILSGAIDYILGNKAAIIT
                     YPVTFLIYTFAIKKWKASQEGRPNQMSQKQVKLAVIIISIIAFLFAFVTNYIGYEGKM
                     NLLAYVTTIAFALSLIANALNALKLTTQWGFWLIYNFVQLTKAGIQGNFANIGKYIFY
                     ILNAIGALFVWNDEEVR"
     gene            complement(19449..20099)
                     /locus_tag="ACL8A1_000676"
     CDS             complement(19449..20099)
                     /locus_tag="ACL8A1_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HGS1201486.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            20211..21053
                     /locus_tag="ACL8A1_000677"
     CDS             20211..21053
                     /locus_tag="ACL8A1_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HGS1201487.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADAQQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            21129..21566
                     /locus_tag="ACL8A1_000678"
     CDS             21129..21566
                     /locus_tag="ACL8A1_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HGS1201488.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            21634..21936
                     /locus_tag="ACL8A1_000679"
     CDS             21634..21936
                     /locus_tag="ACL8A1_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201489.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(22027..23100)
                     /locus_tag="ACL8A1_000680"
     CDS             complement(22027..23100)
                     /locus_tag="ACL8A1_000680"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HGS1201490.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPRDPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILEAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            23256..23933
                     /locus_tag="ACL8A1_000681"
     CDS             23256..23933
                     /locus_tag="ACL8A1_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGS1201491.1"
                     /translation="MHKILLVEDDQVIRQQVGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDNLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            23926..24900
                     /locus_tag="ACL8A1_000682"
     CDS             23926..24900
                     /locus_tag="ACL8A1_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HGS1201492.1"
                     /translation="MLDWKQFFLAYLRSRSRLFVYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLLLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(24949..26892)
                     /gene="thrS"
                     /locus_tag="ACL8A1_000683"
     CDS             complement(24949..26892)
                     /gene="thrS"
                     /locus_tag="ACL8A1_000683"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HGS1201493.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAHEIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(26950..27126)
                     /locus_tag="ACL8A1_000684"
     CDS             complement(26950..27126)
                     /locus_tag="ACL8A1_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HGS1201494.1"
                     /translation="MTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENF
                     LSDKEKQLNVRTRK"
     gene            complement(27133..27273)
                     /locus_tag="ACL8A1_000685"
     CDS             complement(27133..27273)
                     /locus_tag="ACL8A1_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HGS1201495.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDFKWTVEEDLLALSNDIGN
                     FQ"
     gene            complement(27530..27893)
                     /locus_tag="ACL8A1_000686"
                     /pseudo
     CDS             complement(27530..27893)
                     /locus_tag="ACL8A1_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397621.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28129..28758
                     /locus_tag="ACL8A1_000687"
     CDS             28129..28758
                     /locus_tag="ACL8A1_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HGS1201496.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTSDFPEEFKSLAKDQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
     gene            28899..29168
                     /gene="rpsO"
                     /locus_tag="ACL8A1_000688"
     CDS             28899..29168
                     /gene="rpsO"
                     /locus_tag="ACL8A1_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HGS1201497.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            29984..30598
                     /locus_tag="ACL8A1_000689"
     CDS             29984..30598
                     /locus_tag="ACL8A1_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HGS1201498.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(30891..31640)
                     /locus_tag="ACL8A1_000690"
     CDS             complement(30891..31640)
                     /locus_tag="ACL8A1_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HGS1201499.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPINRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            31773..34109
                     /locus_tag="ACL8A1_000691"
     CDS             31773..34109
                     /locus_tag="ACL8A1_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HGS1201500.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAVASILTSYMA
                     HSEDKNPTAHAIRQRFQGQVVYPMLSNLPFSSDRKWGAMELEGLGIVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLNY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELIALAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRRSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEIATLTGQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            34638..36101
                     /locus_tag="ACL8A1_000692"
     CDS             34638..36101
                     /locus_tag="ACL8A1_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGS1201501.1"
                     /translation="MSRKQEQMETLLLLLRDSKDYISAKVLGEKLNCSDKTVYRLVKG
                     INKDCPVEAFILSEKGRGFKLNPRSSLVDVDGNFTEAFDPEVRREKLLERLLLTAPKP
                     HSIYDLGEEFYVSESVVLKDRQILQESLAIYGLDLKMRQRKLFIDGDEAQIRSAILNL
                     LPMFNQLDLEQITQNKVQPLDGELAHFCLGLLITLERELGVNIPYPYNINIFSHLYIF
                     ISRNRRSTSIHVVAPSKPTIVDEKIYSVCQKIIQEIEQYFRMKVDAVEIDYLYQYVVS
                     SRLQKPFSSGKLPFSQRVLDVTHYYFSRMCMDNREIETTDPDFVDLASHISPLLRRLD
                     NRVQIKNSLLSQILLTYPNLVKELTTISKEVSLVFGFASLSLDEIGFLVLYFARFQEK
                     RARPLKTVVMCTSGVGTSELLRARLEKQFTELDIIDVVAYHQLDELINLYPDLDFIVT
                     TVALQEPASVPFVLVSVFLTEGDKQRLQAKIQEINYE"
     gene            36094..36522
                     /locus_tag="ACL8A1_000693"
     CDS             36094..36522
                     /locus_tag="ACL8A1_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGS1201502.1"
                     /translation="MNNLSLVLMDISVQNRQEAYKELANQISLLVSEDTEKIEELLYY
                     RERQGSIEVAKGVLLPHCEGNFQHHVLVITRLKPPIREWSKDIQCVDLIIGLAIAVSQ
                     DKSCIKTLMRRLADESFINQLKQLTKEELREIIYGNQRYS"
     gene            36500..36949
                     /locus_tag="ACL8A1_000694"
     CDS             36500..36949
                     /locus_tag="ACL8A1_000694"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HGS1201503.1"
                     /translation="MEIKDILNVSLIQTDLQMQSKEEVFEALAQLLVETGYVSDRDQF
                     IEGLYQREAEGQTGIGNYIAIPHSKSSAVEKAGVVIAINHNEIPWETIDGKGVKVIVL
                     FAVGDDTEAAREHLKTLSLFARKLGNDEVVAKLVRAQTSDDVIAAFC"
     gene            36977..37288
                     /locus_tag="ACL8A1_000695"
     CDS             36977..37288
                     /locus_tag="ACL8A1_000695"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HGS1201504.1"
                     /translation="MKIVGVAACTVGIAHTYIAQEKLENAAKVAGHVIHVETQGTIGV
                     ENELSQEQIDAADVVILAVDVKISGMERFEGKKIIKVPTEVAVKSPNKLIAKAVEIVT
                     K"
     gene            37314..38399
                     /locus_tag="ACL8A1_000696"
     CDS             37314..38399
                     /locus_tag="ACL8A1_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HGS1201505.1"
                     /translation="MLKHLNLKGHLLTAISYMIPIVCGAGFLVAIGLAMGGGVPDALV
                     AGKFTIWDALATMGGKALGLLPVVIATGLSYSIAGKPGIAPGFVVGLIANSVGSGFIG
                     GILGGYIAGFLVQAIIKKVKVPNWIKGLMPTLIIPFVASLVSSLIMIYIIGAPIAAFT
                     NWLTSLLQSLGSASNGLMGAVIGVLSAVDFGGPLNKTVYAFVLTLQAEGVKEPLTALQ
                     LVNTATPVGFGLAYFIAKLLKKNIYTQEEIETLKSAVPMGIVNIVEGVIPIVMNNLVP
                     GLIATGIGGAVGGAVSLTMGADSAVPFGGVLMLPTMTRPVAGICALLANIVVTGLVYA
                     ILKKPIKHTEPVMTVEEEIDLSDIEIL"
     gene            38410..39105
                     /gene="alsE"
                     /locus_tag="ACL8A1_000697"
     CDS             38410..39105
                     /gene="alsE"
                     /locus_tag="ACL8A1_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose 6-phosphate 3-epimerase"
                     /protein_id="HGS1201506.1"
                     /translation="MSRIEFSPSLMTMDLDKFKEQITFLNDKVASYHIDIMDGHFVPN
                     ITLSPWFIQEVQKISDTPLSVHLMVTDPTFWVDQVLDLQCEYICIHAEVLNGLAFRLI
                     DKIHDAGLKAGVVLNPETPVSTIFPYIDLLDKVTIMTVDPGFAGQRFLESTLYKIQEL
                     RQLRVQNGYHYIIEMDGSSSRKTFKQIDVAGPDIYVIGRSGLFGLDDDIAKAWDICSR
                     DYEEMTGKTMPIK"
     gene            39142..41112
                     /gene="tkt"
                     /locus_tag="ACL8A1_000698"
     CDS             39142..41112
                     /gene="tkt"
                     /locus_tag="ACL8A1_000698"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGS1201507.1"
                     /translation="MSLQSVNAIRFLGVDAINKSNSGHPGIVMGAAPMAYSLFTKHLR
                     ITPEQPNWINRDRFILSAGHGSMLLYALLHLTGYNDVSMDEIKNFRQWGSKTPGHPEV
                     THTSGVDATSGPLGQGISTAVGFAQAERFLAAKYNKDGFPIFDHYTYVIAGDGDFMEG
                     VSAEAASYAGHQALDKLIVLYDSNDICLDGETKDTFSENVRARYDAYGWHTVLVEDGT
                     DLAAISTAIETAKFSGKPSLIEVKTVIGYGSPNKSGTNAVHGAPLGAEETGATRKFLG
                     WDYDPFEVPEEVYSDFKTNVADRGQEAYDAWASLVSDYKVAYPEVASEIDAIVAGKSP
                     VTITEKDFPVYENGFSQATRNSSQDAINTAAAVLPTFLGGSADLAHSNMTYIKADGLQ
                     DKYNPLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAIRLSAIQEL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRSMPNLTVIRPADARETQAAWHHALTSTT
                     TPTVIVLTRQNLVVEEGTDFGKVAKGAYVVYDTPGFDTIIIATGSEVNLAIKAAKELV
                     LQGGKVRVVSMPSTELFDAQDATYKEDILPSKTRRRVAIEMAATQSWYKYVGLDGAVI
                     GIDIFGASAPAQTVIDNYGFTVENIVAQVKSL"
     gene            41481..42082
                     /locus_tag="ACL8A1_000699"
                     /pseudo
     CDS             41481..42082
                     /locus_tag="ACL8A1_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT        12390 a         7710 c         9518 g        12464 t
ORIGIN      
        1 gagattttat tatgaaatta aaaacacttg ctttaattag tggtatcgtc ggtcttgtgg
       61 gaggaatttt acttcttatt ggtccttttg tcttgttggg aacaacggta aacacagctg
      121 ctacaactct taatggagga gctactgcag gggctttttc aggtgttgcc ttactcttga
      181 atgccttgaa gattgaaaat cttgttcttg gtatcattgc tattgtttac tataaaggag
      241 ataagcgtgt aggtgcagct ccgtctgtac taatgattgt ttctggtgga gttagtctca
      301 ttccattctt aggatgggtt ggggggattc ttgctattat cggaggatct ctattccttg
      361 caacattgaa gaaattcaaa tcagaagaat aaaaggtatt ttagcatgaa aagaacaaaa
      421 aagtttatcg gtataggagt agctctatta tctctttctc ttctagttgc atgtggaaca
      481 taaagttcaa agaatacttc aacaagtaat gatgagaaga cagtagcaac atccaatagt
      541 tcaaaagaaa caatcacttt cgatacaccg gttgtaacag acgatgcgat tgaatcaata
      601 cgcacttatg cagattatat agatctttat aaaaatattt ttgatgatta ttttactaaa
      661 gctgaggaag gtttcaaagg cacagctatg gaaaataatg actcgtttac taaactaaaa
      721 gagtcaactc aaaaattatt cgatgcgcag aaaaaaaggt taaataatga agatagaata
      781 gaaacaacca aaaacaatgt gattgccaaa cattgtcaaa cagtcctttc ctttttggtt
      841 ttgactagct tttttgtgaa aaattgtgta aaatagaata gataaacgag gggaaacctc
      901 ggaaaattta aaggagaatc catctaatgg taaaattggt ttttgctcgc cacggtgagt
      961 ctgaatggaa caaagctaac cttttcactg gttgggctga tgttgatttg tctgaaaaag
     1021 gtacacaaca agcgattgac gctggtaaat tgatcaaaga agctggtatc gaatttgacc
     1081 aagcttacac ttcagtattg aaacgtgcta tcaaaacaac taacttggct cttgaagctt
     1141 ctgaccaatt atgggttcca gttgaaaaat catggcgctt gaacgaacgt cactacggtg
     1201 gtttgactgg taaaaacaaa gctgaagctg ctgaacaatt tggtgatgag caagttcaca
     1261 tctggcgtcg ttcatacgat gtattgcctc caaatatgga ccgtgatgat gagcactcag
     1321 ctcacacaga ccgtcgttat gcttcacttg acgactcagt aatcccagat gctgaaaact
     1381 tgaaagtgac tttggaacgt gctcttccat tctgggaaga taaaatcgct ccagctctta
     1441 aagatggtaa aaacgtattc gtaggagctc acggtaactc aatccgtgcc cttgtaaaac
     1501 acatcaaagg tttgtcagat gatgagatca tggacgtgga aatccctaac ttcccaccat
     1561 tggtattcga attcgatgaa aaattgaacg ttgtttctga atactacctt ggaaaataaa
     1621 aaattgtaag cctaggattg atttctaggc tttttatgtt agtatggaag tatgataagg
     1681 aataaaaaac aagattatgt actggcctac aagcaaccag cttcaaccac ttacatgggt
     1741 tgggaagaag aagctttacc gataggtaat ggttctttag gagcaaaagt atttggcctt
     1801 atagggtctg aacggattca atttaatgaa aaaagtctct ggtctggagg tccacttcct
     1861 gatagttcag attatcaggg tggaaatctt caggatcagt atgttttttt agctgagatt
     1921 cggcaggctt tggagaagag agattacaat ctggctaagg aactggctga gcagcaccta
     1981 attgggccaa aaacgagtca atatgggacc tatctgtctt ttggggatat tcacattgag
     2041 ttcagccagc aaggtacgac tttgtctcag gtgacggact atcagagaca gttgaatatt
     2101 agtaaggcac ttgcgacgac ttcttatgtc tataagggaa cgcgatttga acgtgaagct
     2161 tttgcgagtt ttccagatga tctcttggtt caatgtttta ctaaggaagg gttggaaact
     2221 ctagatttta ctatagaact atccttgacc tgtgatttgg cttctgatgg aaagtatgag
     2281 caggaaaaat ctgattacaa ggagtgtaag ttggatatta ctgattctca tatcttgatg
     2341 aagggaagag ttaaggataa tgatctgcgg tttgctagtt atctagcttg ggaaacggat
     2401 ggagatatta gagtttggtc agatagggtt cagatatcag gagccagtta tgccaatctc
     2461 ttcttggccg ctaagacgga ttttgcccaa aatcctgcta gcaattatcg caagaaacta
     2521 gatttagagc aacaggtgat agacttggtg gacacagcta aagaaaaggg ctatacccaa
     2581 ttgaaatcaa ggcatatcga ggactaccaa gccttattcc agcgtgttca attggatttg
     2641 gaagctgatg ttgacgcatc cactacagat gatttgttaa aaaattataa gccacaagaa
     2701 gggcaggctt tggaggagct gttcttccag tatggacggt atttattgat tagttcgtcc
     2761 agagactgcc cagatgctct accagctaac ctacagggag tctggaatgc ggtcgacaat
     2821 cctccttgga attcggacta tcacttaaat gtcaatctgc agatgaatta ttggccagcc
     2881 tatgttacca atctcctaga gacggtcttt ccagtcatca actatgtaga tgatttgcgt
     2941 gtctatggtc gtctagcggc tgtaaagtat gcaggaatcg tctctcagaa aggtgaggaa
     3001 aatggttggt tggttcatac tcaagcgact ccctttggtt ggacggcacc tggttgggat
     3061 tactattggg gttggtcacc agctgccaat gcgtggatga tgcaaaccgt ttatgaagcc
     3121 tattcatttt atagggacca agactatctt agggagaaaa tttatcccat gttgagggaa
     3181 acggttcgtt tttggaatgc ctttttacat aaggatcagc aggcgcagcg ttgggtgtct
     3241 tctccgtctt attctccaga acatgggccg atttcgattg gcaataccta tgaccaatct
     3301 ctgatttggc agttatttca tgattttatt caggctgctc aggaattggg actggatgag
     3361 aacttgttga ctgaggttaa ggagaagtct gatttactaa atcctttgca aatcactcaa
     3421 tctggtcgaa tcagggagtg gtatgaggag gaagagcagt attttcaaaa tgagaaagtg
     3481 gaggcccagc accggcacgc ttcccatcta gtgggactct atcctggcaa tctctttagc
     3541 tacaagggac aagagtatat tgaagcggcg cgtgctagcc tcaatgatcg tggagatggc
     3601 ggcacaggct ggtccaaggc taataagatc aatctctggg cgcgtttggg agatggcaat
     3661 cgagcccata aattattggc agagcagtta aagatatcca ccttgccaaa tctttggtgt
     3721 agccatcctc cttttcagat agatggtaat tttggtgcta ctagtggcat ggcagaaatg
     3781 ttactccagt ctcatgcagc ttatctggta cctctagctg ccctacctga tgcttggtca
     3841 acaggttctg tttcaggctt aatggcacgt ggacattttg aagtgagcat gagctgggaa
     3901 gataaaaaac tcttacagtt gaccatttta tcaaggagtg gaggagattt gcgagtttct
     3961 tatccagata ttgagaagag tgtgattaaa atgaatcaag aaaaaataaa agcgaaatgc
     4021 atggggaaag attgtatttc ggtggcaaca gcagaaggtg atcttgttca attttatttt
     4081 taagaagatg ttataaggca gtaatttgaa actgcctttt aataaggatt taagaatata
     4141 ggcagtttcc aactagttga aaaagcgtta taatgataat aggaagtaat actcaatgaa
     4201 aatcaaagag cacaaactag gaagctagcc gcaggttgct caaaacagtg ttttgaggtt
     4261 gtagatggaa gctgacgtgg tttgaagaga gattttcgag gagtataatt tgtttgatag
     4321 agggtgggtc tgatggctta tattgagatg aaacactgtt acaagcgtta tcaggttggg
     4381 gacacggaga ttgtggccaa tcgtgatgtg aattttgaga ttgaaaaggg ggagctggtt
     4441 attatccttg gtgcttcagg tgcaggcaag tcaacagttc ttaaccttct tgggggaatg
     4501 gataccaatg atgaagggga aatctggatt gatggtgtta atattgcgga ttatagttcc
     4561 caccagcgca ccaattaccg tagaaatgat gtggggtttg tttttcagtt ttataatcta
     4621 gtttctaatc tgacagctaa ggaaaatgtg gaactggctt ctgaaattgt gacagatgcc
     4681 ttgaatcctg ctcaggtttt gacagatgta ggtctggctc atcgtctcaa taactttcca
     4741 gcccagcttt ctggagggga gcaacagcga gtctccattg cacgcgcggt agccaaaaat
     4801 cctaaaattc ttctttgtga tgaaccgact ggagccttgg attatcagac gggcaagcag
     4861 gttttgaaaa ttctccaaga tatgtctcgt caaaagggag cgacggtgat catcgtgact
     4921 cataatggag ctttggcgcc cattgctgat cgcgtgattc atatgcacga tgccagtgtc
     4981 aaggatgtgg tgctcaacca gcatcctcag gatattgaca gtttggagta ctagcatgat
     5041 caagcgaaaa acttattgga aggacttaat tcagtccttc acaggctcca aggggcgttt
     5101 tttatccatc ttgatcctga tgatgttggg atctctagcc ttagtaggcc tcaaagtaac
     5161 cagtcccaac atggagacga cagctaatgc ttatttaaca actgctcaaa ccttggattt
     5221 ggcagtcatg tctaactatg gcttggatca agcagaccaa gaagaactaa aacagacgga
     5281 gggcgcagag gtcgagtttg gctatttgac agatgtgact atggataatg ggcaggatgc
     5341 cattcggctg tactccaaac cagagcgaat ttcaaccttt cagctaagaa agggacgact
     5401 tcctcagtca gacaaggaaa tcgctttggc cactcatttg caaggccaat acagcgtggg
     5461 acaggagatt agttttaaag aaaaagaaga gggtcattcc tctttaaaag accatactta
     5521 taccattact ggttttgtgg attcggctga aatcctctcc cagcgagata tgggctacgc
     5581 aggaagtgga agtgggactc tgacagccta tggggtgatt ttacctagtc aatttgatca
     5641 gaaagtctac aatatagctc gtttgaaata tcaagattta gcaggtttaa atgccttttc
     5701 atcagcttat gaagaaaaat ccaagcaaca tcaggaagag cttgaacaaa ttttatcaga
     5761 taatggcaag gtacgtctgc aacttttgaa aaaagaagga caagagtctc tagacaaggg
     5821 gcaagagacc cttgacaagg ctcagactaa tttgcaggaa ggcaagcgtc gtttagcagc
     5881 tgctcaagct cgtatacagg ctcaagaaag tcaactagcc ttgtttcctc aagttcagag
     5941 agagcaggct agtgctcaac ttacccaagc caagcaggaa ttgggcaagg aagaggccaa
     6001 actaaagcaa gctgaacaaa atctagccca agaaaaggaa aaattagaaa aacatcagca
     6061 agtcttggac gatttggcgg agccaaggta tcaggtctat aatcgtcaga ccatgccagg
     6121 tggtcagggc tatcttatgt atagcaatgc ttcatccagt attcgagcag tgggcaatat
     6181 ctttcctgtg gtactttatg ccgtagcagc catggtgacc tttacgacca tgactcgctt
     6241 tgtagacgaa gagcgaactc atgcagggat ttttaaggcc ttgggttatc gtagtaagga
     6301 tattatcgcc aagtttctcc tttatggact agtagctggg actgtcggaa cggctctagg
     6361 tagtatactt ggtcattatt tgctagccaa tgtaatttca agtgtcatta caaaaggcat
     6421 ggtggtggga gaaactcaga ttcagttcta ttggacctat agcttactag ctcttgtctt
     6481 gagcttgttg gcgagtgtgt taccagccta tctggtggct tggagggaac ttcatgacga
     6541 agcagcccag cttctacttc ctaaacctcc tgtcaaagga gctaaaatct tattggagcg
     6601 tatcggtttt atctggcgtc gtctcagttt tactcataag gtaacagctc gcaacatctt
     6661 tcgttataag cagagaatgt tgatgacaat ctttggtgtg gcaggttctg tagctctgct
     6721 ctttgcaggt ttgggaatcc aatcttctgt agcaggagtt ccgtctaaac agtttcaaca
     6781 aatccaacag tatcagatgc ttgtctctga aaatcctagt gcgaccaatc aggacaaggt
     6841 agagctagca gaagtgttga aagggcagga gatactagcc taccagaaaa tctattctaa
     6901 agcgctagac aaggatttca aaggcaaggc tggtcttcaa aacattactc ttatgatgat
     6961 agagaaggaa gatttgactc cctttatcca tcttcaacat catcagcagg agctgacatt
     7021 aaaagatggc atcgttatta cagctaaact cgcccagctg gcaggtgtca aggttgggca
     7081 gactttagaa attgaaggta aggaactaaa ggtcgctgct attactgaga actacgttgg
     7141 tcactttatt tatatgagtc aggctagcta tgagcaactt tacggacagc taccccaagc
     7201 caacacttat ttggtctcat taagggatac cagtgcaact agtatcgaaa gtcaggcggg
     7261 cttgcttatg aatcaatctg cggtgtccag cgttgtccaa aatgcttcag ccattcgact
     7321 cttcgactct atcgctagct cactcaatca gaccatgacc atcttggtca tcgtatcggt
     7381 tctattagct attgtcatcc tttacaatct gaccaatatc aacgtagctg agagaatccg
     7441 tgaactctcc actatcaagg ttctcggttt tcataataat gaagtcaccc tctacattta
     7501 ccgtgagacg attgtgctgt cccttgtggg aatcgtactt ggtctggtag ctggtttcta
     7561 tttacaccaa tttttgattc aaatgatttc gcctgcgact attctctttt atccgcaggt
     7621 aggctgggaa gtctatgtaa tcccagtggc aacagtaagc atcattttga ccttgcttgg
     7681 tttcttcgtc aattattatc tgagaaaggt tgatatgtta gaagccctga aatctgtaga
     7741 gtaaggtagt tatttttagc tgattgaact tctatttact aatattcaaa aatccttcgt
     7801 ttcaaagagc agggagctct ttgtgacaga ggattttttc tatagggctt tagcagctgc
     7861 aattgcggct tcgaagtttg gctcagaatt gatattatcc acgtattcaa cgtagcgaat
     7921 cgtattgtca gtatcgagga caaagactgc gcgtgctaat aggtgccatt cgttgatcaa
     7981 gagggcataa tcgcgcccga aagaatggtc aaagtagtct gaaagcataa tggcattgtc
     8041 aaggccttca gcaccgcacc aacgtttttg agcaaaaggt aggtccattg aaacagtcaa
     8101 tacgaccgtg ttgtccagtc cagccaattc ttcattaaaa cgacgtgttt gagttgagca
     8161 gatacctgta tcgatagaag gaacgacact caagactttt ttcttgccat caaaatcagc
     8221 cagagatttt ttagaaagat ctgttgtagt aagagaaaaa tcaagcgcct tgtcgccgac
     8281 ttgtagttgt ttacctgtaa agctcacagg atttccgaga aaagttacca taggatactc
     8341 caatcttttt tcttccattg tatctaaaac agtcagaatt ttccaatgat ttgaccggaa
     8401 atgtgtgcat agaaaaaacg ccagctcaca tgagaatgac gtttttcaga ggcttatttt
     8461 gccaatcctt cagcaatctt gtcaaggttg tatttcatca tgctgtagta gctgtcgcct
     8521 tctttacctt gttctgcgat agagtcagta aagatttgtg cgtagattgg gatgtttgtg
     8581 tcttgagaaa cagttttcat tggacggtca tccacacttg attctacaaa gagtgatgga
     8641 acttttgttt ggcgaagttt ttcaaccaag gtcttgattt gttcaggagt tccttcttct
     8701 tcagtattga tttcccagat gtaggcactt gggacaccat aggctttaga gaagtatttg
     8761 aatgctcctt cgctggttac aatgagtttc ttttcagcag ggatcttatt aaatttatcc
     8821 ttactttctt tatcaagttt gtctaactta tcagtatatt ctttgagatt tttttcatag
     8881 aattctttat tgttagggtc tttggcgctc aattgtttgg cgatattttt agcaaaaata
     8941 ataccgtttt caaggttaag ccaagcgtgt gggtcttctt ttcctttttc attttgacct
     9001 tcaaggtaga taacatcaac gccgtcgctg actgcgaagt agtctttgtt ttcagttttc
     9061 ttggcatttt ctaccaattt tgtaaaccaa gcattgccac ctgtttcaag gttgataccg
     9121 ttatagaaaa tcaaatcagc ctcagaagtt ttcttaacgt cttcaggaag tggttcgtat
     9181 tcgtgtgggt cttgcccaat cggaacgata ctatgaaggt caattttgtc accagcaata
     9241 tttttagtaa tatcagcgat gattgagttt gtagcaacaa cttttagttt ttgaccagaa
     9301 gttgtatctt tttttccgct agcacatgct acaagaatga ttgcagaaag aaagagaacg
     9361 agtaatgtac ctaatttttt cattagatcc tccaatttat tagggctttg ccccttattt
     9421 taacaaatgt ttatttttca gtttcaaata tcgttgtttg ggagcgataa agaagctaat
     9481 gagaaagaaa ctagcagctg taagcacgat actagaacct gccgcaacat taaaactata
     9541 gccaataaag agtcccaaaa ctgaagcagt agctccgaag gttgaggaaa ggaaaatcat
     9601 acttttcaga ctattagcat acagataagc agttgcagct ggggtaatca gcatggctac
     9661 aatcaggata gttccgacac tttgcatggc tgtcacagac acgagagtca ggagtaccat
     9721 gagaaggtag tgatagaaat tgacaggcat tcccatggct ttagccaaga gttcatcaaa
     9781 ggaagttatc aagagttgct tgaagaaaat ccagattaac aagaggatgg ctgcccccac
     9841 acccatagta ataaacatat ccgtatcttg gacggccagg atattaccaa aaaggatatg
     9901 gaaaaggtca gttgaacttt tagcgacacc aatcaagatg ataccgaggg ctaagaaaga
     9961 agaaaaggta atgccgatgg cggtatcgct tttgataatc gagtttcctt tgatgtaggt
    10021 aatgatgatg gcagctagca atccaaagac aatggctccg ataaagaagt caaggcccaa
    10081 gatgaaggag agggctacac ctggtaagac agcatgtgaa atggcatctc ccatgagtga
    10141 catcccgcgt agaataatga aacatcccac agctccagct acaatcccga cgacaatagc
    10201 tgttatcaag gcattttgta ggaaatggaa tttttgcaat ccatcgataa attctgcaat
    10261 cataggtcac ctccattgaa aaagagttga ttaccgtaag cttcttttag attggtttcg
    10321 gtaaaagttt cttttgttgg accaaaggca atcacttctc gattgacaag taagacttga
    10381 tcgaagtagt ggggaatctt gctgaggtcg tggtgaacga tgagaaccgt cttcccagct
    10441 tttttcaaat ctctcagcgt attcatgatg atttcctcac tgacagagtc aatcccagca
    10501 aagggttcat ccaagaggat atagtcggct tcctgcacca aacatctggc aatcaagacc
    10561 cgctggaatt gacctccaga cagttgacta atttgacgtt cagcgtagtc agctaggccg
    10621 acgatttcaa gggcctcttg cactttcttc caatgtttag cctttaaact tcgaaagaga
    10681 ggaatagagg gaaatagtcc taacgagacg cattccttga ccttgatggg aaagttgtag
    10741 tcgatattga ttttttgttc gacataggca attcggtgta aggatttttt aacttccttg
    10801 tcatcgagaa atgcctgacc ttgatgtggg ataattccca gcataccttt taatagtgtt
    10861 gatttcccag cgccatttgg accaatgatg ccagtaattg ttggtccatg gagcactagt
    10921 gaaatatcct taagtgccaa cgtttctttg taggagacac tgaggttttc gatacgtatc
    10981 ataaacttgt attcctcctg tctcttaata tacattaaaa aaaaaattaa gtcaagttaa
    11041 tttttgaaaa aattaaaata ataactgaaa aatagattct aaagataact ttcaggataa
    11101 atttctaaat tataaaacgc atagtatcaa gtgtaaaaaa cttggaatta tgcgttttat
    11161 catggaaagg ttttttataa tagctaaaaa ataatagtta aatggttatt aattgcattc
    11221 cctagtgatt tttgttaaga taaatgcaaa tacaaatgaa agcgagaaca agatgacacg
    11281 ttatcaagat gatttttatg atgctatcaa tggagaatgg caacagacag ctgaaatccc
    11341 agcagataag tctcaaacag gaggttttgt tgatttagac caggaaattg aagacctgat
    11401 gctggcgaca acagacaagt ggttagcagg tgaagaagtg cctgaggatg ctatcttgga
    11461 aaactttgtc aaataccacc gcctagttcg tgattttgac aagagagaag ctgacggtat
    11521 cacacctgtc ttaccactcc ttaaagaatt ccaagaattg gagacttttg cggattttac
    11581 agctaaacta gcagagtttg agcttgcagg aaaaccaaac ttccttcctt ttggtgtatc
    11641 gccagacttt atggatgcta gaatcaatgt tctatgggct agcgctccaa gcacaatctt
    11701 gccagatacg acctactatg cagaagaaca tcctcagcgc gaagagctct tgactctttg
    11761 gaaagaaagc agcgcaaatc tcctcaaggc ttatgatttc tctgatgaag aaattgaaga
    11821 cttgctagaa aaaagacttg aattggaccg ccgagttgcg gcagtggtgc tctctaatga
    11881 agaaagttca gaatatgcta aactctatca tccatattct tacgaagatt tcaagaaatt
    11941 cgcgcctgcc ctacctttgg atgacttctt caaagcagtt attgggcaat taccagacaa
    12001 ggttattgta gacgaggaac gtttctggca agcggcagag caattctaca gtgaggaagc
    12061 ctggtctctc cttaaagcaa ccttgatttt gagtgttgtc aatctttcaa ccagctattt
    12121 aacagaggat atccgtgttt tgtctggtgc ctacagccgt gccctttctg gagttccaga
    12181 ggcaaaagat aaggtcaaag cagcttatca tctagcacaa gaacctttca agcaagccct
    12241 gggtctttgg tacgcccgtg agaagttctc tccagaagcc aaggcggatg tggagaaaaa
    12301 agtggcaacc atgattgatg tctataagga gcgtctgctt aagaatgact ggctcactcc
    12361 agaaacctgt aaacaggcta tcgtgaagct caatgtgatc aaaccttata ttggctatcc
    12421 agaagaattg cctgcacgtt acaaggataa ggtagtgaat gaaactgcca gtctttttga
    12481 gaatgctcta gcctttgcgc gtgtggaaat caagcacagt tggagtaagt ggaaccagcc
    12541 tgtagactat aaggaatggg gcatgcctgc tcatatggtc aatgcctact acaatcctca
    12601 gaagaacctg attgtctttc cagcggccat tttacaggcg cctttctatg acttgcatca
    12661 gtcatcttct gctaactacg gtggtattgg ggcagtgatt gcccatgaaa tttcccacgc
    12721 ctttgatact aacggagctt cctttgacga aaatggtagc ctcaaggatt ggtggacaga
    12781 gagcgactat gctgccttca aggagaaaac acaaaaagtc attgaccaat ttgatggaca
    12841 ggattcttat ggagcaacca ttaacggtaa attgactgta tcagaaaacg tggctgactt
    12901 gggaggaatc gcagcagcgc ttgaagcagc taagagagaa gcagacttct cagcagaaga
    12961 gttcttctac aacttcggtc gcatctggcg catgaaaggt cgaccagaat ttatgaaact
    13021 tttggctagc gtcgatgtgc acgcaccagc caaactccgt gtcaatgtgc aagtaccaaa
    13081 cttcgacgat ttctttacaa cctatgatgt caaagaagga gacggaatgt ggcgttcacc
    13141 agaggagcgc gtgattattt ggtaatacaa aaaatctagt catcagaaaa aaggcatcca
    13201 atcacgtgtg aaaaccttga taggatgcct tttgtaatag aaagatttgc tggatagttt
    13261 acttaaattt cgattgtcat catcttgcta gaaaaaggga ttgaaggcct tttcgtgagc
    13321 gatagtagta gctggaccat gtcctggata gacatcgtag tttggtaggg tgaagagttg
    13381 ggtctggata ctatgaagga gttgctccat gctaccagtc ggaaggtcgg tccgtccgat
    13441 agtttcgcgg aatagagcat ctcccgtcaa gactaggtga gcatcaggaa agactaggga
    13501 aacaccaccg atagagtgcc ctggggtcgg tagaacttta aaacgaaatt cctctagttg
    13561 gtattcttcg tgaaagacaa aggtgtgttc tgcaggtttt gtgaccacat ctgccatatc
    13621 atcgtggcga gggagaccgg agagattatc gacaggagtg tagagccagc tggcttcgct
    13681 ctctgcgata tagacaggag gattgccaaa cgtctcgcga accaagtcca gactcatgat
    13741 atggtcataa tgggcgtggg tcaagagaat agcacagatc ggtttgttga tcttctcgat
    13801 tgtctgacga atggcttccc aatgactacc aggatcgacg acgatgaggt gcttttcgcc
    13861 ttctagataa taggtatttt cataggcaac aggattcacg gttttatgga ttttcatact
    13921 agctccaatc tcaaagaatg tactaatact cttcgaaaat ctcttcaaac cgcgtcaact
    13981 ttatctgcaa cctcaaagca gtgctttgaa cagcctgcgg ctagctttct agtttgctct
    14041 ttgattttca ttgagtataa attaagtata gcacagttag ggagaatagg taaggattta
    14101 aaatgaactt aaaaaccagc atgactagat gaagacaggc tggttatcaa tttatcaata
    14161 ttggaaaggt gtcaatttcc cttcataggt tgcataaact ccgtcattag cttgtttgat
    14221 agacgtgatg tttagagtgc taccgtagtt ggcttctcat ttgtcgctat attcactata
    14281 agttatccta ctgggcaggt tctggtcgtt agcatagtat tgaacgagtt ttttatagct
    14341 ttgctggctg aataggaaca ggcagcaaac tcctaacatt aggagagcaa agatagaaat
    14401 agcagttttt ttcataggta ttctccttat aaactaaagg atagaagaaa gtatcttgag
    14461 gatttttata ctcttcaaaa atctcttcaa accgcgtcaa cgtcgccttg ccgtatatat
    14521 gtgactgact ttgtcagtct tatctacaac ctcaaagcag tgctttgagc aacctgcggc
    14581 tagtttccta gtttgttctt tgattttcat tgagtattaa ttccaaaatc cagtcaaggt
    14641 accagtgtat gtgatacgag ttccaccgtt tatctttcgt atcttcaatg gaattgggga
    14701 agtcagaaag tctttgggtt acaatatggt ttccgttctt ctatctctcc ttctatcacc
    14761 taagtttaaa atctaacttc gtagtatatt atgggccaat aattcagaat tatcaagcaa
    14821 ttttgaatta tttttttctt attctgttcc aatctagttt tcatattctt aaaaaagtga
    14881 taaaatggta ggaagaattg gagagtagag atgccgaaag aagtgaattt aacaggcgaa
    14941 gaagttgtcg ctttaaccaa agaatattta acggaagagg atgttcattt tgtccataag
    15001 gccttggtct atgctgttga atgccacagt ggtcaatatc gcaaatcagg cgagccttat
    15061 atcattcacc ctatccaagt ggcaggtatt ttagctaagc taaagctgga tgctgtaaca
    15121 gtagcttgtg gattcttgca tgatgtggtg gaagatacag atgcgacttt ggacgatttg
    15181 gaaagagagt ttggtcctga tgtgcggatg attgttgacg gagttaccaa gcttggcaag
    15241 gtcgagtaca aatcgatcga ggagcaatta gcggaaaatc atcgcaagat gctcatggcc
    15301 atgtctgagg acatccgcgt tattttggtc aaactgtctg accgcttgca caatatgcgg
    15361 accctgaaac atcttcgaaa agacaagcag gagcgtattt ccaaagaaac catggaaatc
    15421 tatgccccac ttgcccatcg tttggggatt tccagtgtca aatgggaatt agaagacttg
    15481 tctttccgtt atctcaatcc aacggagttt tacaagatta cccatatgat gaaggaaaag
    15541 cgcagggagc gtgaggcctt ggtggatgag gtagtcacaa aattagagga gtatacgaca
    15601 gaacgtcact tgaaagggaa gatttatggt cgtcccaagc atatttactc aattttccgc
    15661 aaaatgcagg acaagagaaa acggtttgag gaaatctatg atctgattgc tattcgttgt
    15721 attttagata cccaaagtga tgtttatgcc atgcttggtt acgtgcatga attttggaaa
    15781 ccgatgccag gtcgcttcaa agactatatt gccaaccgca aggccaatgg ttatcagtct
    15841 atccatacga ctgtttatgg accaaaaggg ccgattgaat tccagattcg aaccaaggaa
    15901 atgcacgagg tggctgagta cggggttgcg gctcactggg cttataagaa aggtataaag
    15961 gggcaagtta acagcaagga atcagctatt ggaatgaact ggatcaagga gatgatggag
    16021 ctccaagacc aggctgatga tgctaaggaa tttgtggact ctgttaagga aaactatctg
    16081 gctgaggaga tttacgtttt taccccagat ggagctgtcc gttctcttcc caaagattca
    16141 ggaccgattg attttgccta cgaaatccat accaaggtcg gtgaaaaagc aactggtgcc
    16201 aaggtcaatg gccgcatggt tccactgaca accaagttaa agacagggga tcaggttgaa
    16261 attatcgcca acccgaactc ctttggacct agccgtgact ggctcaatat ggtcaagact
    16321 agcaaggcgc gcaataagat tcgccagttc tttaaaaacc aagataagga attgtctgtc
    16381 aacaagggtc gtgagatgct gatggctcag ttccaagaaa atggctatgt ggcaaataaa
    16441 tttatggaca agcgccacat ggatcaagtt ctgcaaaaga ccagttacaa gacagaagac
    16501 tccctctttg cggccattgg ttttggggaa atcggtgcga ttaccgtctt taaccgtctg
    16561 actgaaaagg aacgccgtga ggaagagcgt gccaaggcca aggctgaggc agaggagctt
    16621 gtcaaaggtg gcgaggtcaa ggttgaaaat aaagaaactc tcaaggtcaa gcatgagggg
    16681 ggagtggtta ttgaaggtgc ttctggtctc ctagtgcgga ttgctaagtg ttgtaacccc
    16741 gtgcctggtg acgatattgt tggctacatt accaagggtc gtggtgtggc tattcaccgt
    16801 gtggactgta tgaacctgcg tgcccaagaa aactacgagc aacgtctcct tgatgtggaa
    16861 tgggaagacc agtactctag ctcaaataag gagtatatgg cccatatcga tatctacggt
    16921 ctcaaccgta caggactgtt gaacgatgta ctgcaagttc tttcaaatac aaccaagaat
    16981 atttcaacgg tcaatgccca accaaccaag gatatgaagt ttgctaatat ccatgtgtcc
    17041 ttcggtattg ccaacctctc tacactgacc acggttgtcg ataaaattaa gagtgtgcca
    17101 gaagtttact ctgtcaaacg gaccaacggc tagttgtcct agctcttact agaaaggcta
    17161 ttatgaaaat cattatccaa cgggttaaaa aagcccaagt gagtatagaa ggtcagattc
    17221 agggaaaaat caatcaggga cttttattgc tggttggtgt tggaccagag gaccaagagg
    17281 aagatttgga ctatgctgtg agaaaactgg tcaatatgcg gattttttca gacgcagaag
    17341 gcaagatgaa cctgtctgtc aaagatattg aaggagaaat cctctctatt tctcagttta
    17401 ccctctttgc ggatactaag aaaggcaatc gtccagcctt tacaggggca gctaaacctg
    17461 atatggcatc agacttctat gatgctttca atcaaaaatt agcgcaagaa gtgcccgttc
    17521 agacaggcat ctttggagca gatatgcagg ttgagctggt taataacgga cctgttacca
    17581 ttatcctaga tactaaaaag agataagaaa gaccaagccc agtcggcttg gtctttctca
    17641 tctatcataa aatactccaa aaagaaatcg gttcttgata tgcttggggg aagtctcttt
    17701 tcaggctttg gcagatgcga taggaaggga tgagatgtcc taggatgagg agagttccct
    17761 gaaggaaaag tggaatatca cttaaagaca ccaaggagag aatcatcagg ctatcccata
    17821 ggagaatttg taaagcaaaa cgccagaatc tcggtctaat ctgggaatag agttaactaa
    17881 agtggtcaca aagttggttt gaaagatagg tcaatagcac aggatgaaag aaaatgagcc
    17941 aaccgctata aatcaaaatc cagtcccaag taagcccccc ttttaaatgt caaaaatgcc
    18001 agcatgattc catcaatgac aaggagttga atggttttga tgaagatgat ttttttcatg
    18061 ctaaaacctc cttttcctta gagacactcc cataaagaga tgtgtttgtc gtaaaagtct
    18121 ggatagtttt ctcttaggta gtcagctgtc atataataaa aagtagaatg gcaagcagtg
    18181 atgattggca taagagatga aaaacgctga gaactaatga aggctaagag aacaaaaaat
    18241 agaaaatcaa tggtgagaac ccctaaatag tagaagcgaa tctttttatt gatttgagga
    18301 taaatctcag cgaattgatg tttgagtcgg acgatcaaga gaaatagcag gagtccttga
    18361 gcaaggaaga aaattaaacc agagagcaag agccactcaa aagatgtctc gagtctaatg
    18421 ataaaaaatg caaatagtag caaatcaata actgcaattg ctataaaatg gattctatag
    18481 agttttttca tgacaagacc tccctctttt atctaacttc attctactcc aaaagaatgg
    18541 gagttacaac taaaatgata aaaatagcag aagggagatt ctcttaagtt ggctagtatt
    18601 ctttatttga gtttccttct attatctaac ttcttcatca ttccagacaa ataaagctcc
    18661 gattgcattg aggatataaa agatgtattt accgatattg gcgaagtttc cttgaatacc
    18721 agcttttgtc agctgaacga aattgtaaat caaccaaaag ccccactgag ttgttagttt
    18781 taatgcattc aaagcattgg caatgaggga tagtgcaaag gcaatagttg ttacgtaggc
    18841 aaggagattc atcttgccct catatccgat atagttggtc acaaaggcaa agaggaaggc
    18901 gatgatggaa atgatgatga ccgccaattt tacctgtttt tggctcattt ggttgggtct
    18961 gccttcttgc gaagctttcc atttctttat agcaaaggta taaatgagga aggtgacggg
    19021 ataggtaatg atggccgcct tatttccaag gatataatca atagcaccgg acaaaatggt
    19081 attaacaata ctaaagtaat ttccccattt gcttaatttc cccgtgaaac gagtggacaa
    19141 catggaaatc ccaacgttgg ttacggaaat caatccaaag ggtacaagag ctgtccatgg
    19201 tccccagtct acaaatttat cgaggcgtga gttgaggtaa ccagatgcaa tcgcaatccc
    19261 aacgaccaaa gcaaccccga agaggtcaaa ccatttagat gtagcaaaaa tttttagtga
    19321 ttttttcata ggttaaacta cctttctttt ttttttcaaa tattctacca ccaaatgaaa
    19381 gtaaaataaa atgatagaaa taaaaccctg aaaataaaac tttcagggtt ggtaggggac
    19441 ttgagaaatt agttgatttt ctcgacatag agttgttttg caatgtccat attcacttgt
    19501 aaatcctcgt cgttactgag gatagtgaag gtattgctga agccatcaaa ctgcttgact
    19561 tggagctggt caccgatgtg aagtgagtgc ttgtccagat aatggagaat gtcaaaacta
    19621 tcgtgcaccc gagtcaggcg gtaggcgcca gcttccttga tatcagctag tgggaggtta
    19681 ttgatttcaa cgagtagttc tcccttggca ggaatagttc ctccgtgggg gcaggtttta
    19741 gggaaaccta gcagtttatc tagtctttcc acgaacaggt cagagacagt gtgttccaag
    19801 acctcagctt cctcgtgaat ctggtcactt gtatagtcta aatgatgaac tagaaaaact
    19861 tcaatcaagc ggtgcttacg atagagctca gagaccagtt tgagaccgag gtcagtcagt
    19921 agatagccac attccttgtc ctttaggatg agattttcac ttttcattcg tttgatcatt
    19981 tcagttacgg cagggggaga gacttgcatg cgagccgcaa tttccttgtt ggtaatctta
    20041 tgcaggtcta tgccaatttc ataaatacat tttagatagt cttctttgtt tggggtcatt
    20101 cgtttcctct tgtctaatat ctttatctag tatatcaaaa aaagctagat ttctctagct
    20161 gtgacttttt atgttatact tattgcaaga gaaaaaacga ggagatggat atgacgaaaa
    20221 tagctcttct ttcagatatt catggaaata ccaccgcctt ggaggctgtt ttggcagatg
    20281 ctcagcagct aggagtggat gaatactggc ttttgggaga tattctcatg ccagggacag
    20341 gacgtagaag gattttggac ttgttggatc aactaccgat tacggctaga gttttgggaa
    20401 actgggaaga cagtctttgg catggtgtcc gtaaggaatt ggacagtact cgccccagtc
    20461 aacgctatct cttgcgccag tgccagtatg ttttagagga aatttcccta gaagaaattg
    20521 aagtgctcca caatcaacct cttcaaattc atcgtcagtt tggggatttg acggtgggaa
    20581 ttagccatca tctgcctgat aagaactggg ggcgagagtt gattcatact ggcaaacaag
    20641 aggagtttga ccgcttggtg actcatcctc cctgtgatat tgctgtttat ggacatattc
    20701 accagcagtt gcttcgttac gggactggtg ggcaattgat tgtcaatccg ggttcgattg
    20761 gccaaccttt ctttttggac gctcagctac ggaaggactt gcgggcccag tatatgattt
    20821 tggagtttga tgacaaaggc ctggtagata tggacttccg acgggtagac tacgatgtgg
    20881 cagcggaatt gcagctggct aaagacctga gacttcccta ttttgaggtt tactatgaaa
    20941 gtctggtcaa tgggattcac catactcatc atcaggaatt tttgagagaa ttggctcaga
    21001 aagagggctg cgaccgggag ttagacgact ggttgaaaag tggtaacgat tgacattaca
    21061 actaaaaagg gtataataat agaaaaagaa gagtagaggc agggtagcct cgtaaaaagg
    21121 agaagaggat gcaaattcca agtagattta ccattgcgac tcatatgctg ataatcattg
    21181 ccctcgaggg gaaggaaagc aaggtgacca gtgattttct ggctgctagt gtcggggtca
    21241 atcctgtcat tatcagaaag atcttgtccc agttgaagaa ggcagagctg atttcagtag
    21301 cgcgtggaac gggcggaaca gagattgtca aggaccttaa ggatattagt cttttagatg
    21361 tttatcaggc ggtcgaatgt cttggtaaga caggtcaact cttcagtttc catgacaatc
    21421 cgaatccaaa ttgccctgta ggagctcata ttcatgatgt tttggatcaa aaattggaga
    21481 gaattcagtt gactatggag gcagaacttg gtcaaaccag tctagaaaaa gtcgtggccg
    21541 atgcagagag tcagatgaag gattaagagg gataaatgcc ctcttttttc tttggataat
    21601 tatgataaaa tgtagctatt aaaggagtaa gaaatgtatc tcgttatagg aattgtatta
    21661 gcctttatag tgtcattttg gaaggataat agaagtttgt ggaatccagt attattttta
    21721 ttatccctca tttcaagtta tttttatcta tcctaccttt tttataaaaa tggatacgag
    21781 aatgttcagt tagcttttta tgtatttgcc tttgtcttac ttcctttttt gctttttcta
    21841 agtggcatct ttttaatcta taatggggtc attttgttga agcgagaggg acgttccaag
    21901 ccccattatc tctcaatgct atttgatttt tattgactat tagattctat ttaaaaagac
    21961 ttagaatgca catgaaagta tgtattctag gtcttttgtt ttgtatacat ttaattttca
    22021 agagtattat ctatttttcc atgcctggta acgagtatca atcaataact gggtaaggcg
    22081 acccaaggtt tctctttctt ctggagtgag ggattgagct tggacaaaga gatgatgaat
    22141 gtattcaagc gcctttaagg aagccaagtc attgatagag ctaataccag cctcaagaat
    22201 agtgccaaag aagaggtcga agtagagctg aagttcttcg tcagggactg tggccaccca
    22261 ttctttaaag gttgtgtcta cttgctggct atcactgttg gtcttatcca gttggacgaa
    22321 gtgcttatcc tcaatctgcc aactaaaggt atcgtgctgg gcgatgccac ccagggcagt
    22381 actctgaacg atgatttggt gagcaggaat ttccagcatc ataccgataa tggaaccttg
    22441 tggaatgaat atcttgcttc tatccattat cctttgataa cccgcagtct gtgtcaattc
    22501 ttgatggaga ccaggtgcat caaatgtata aactgctgtg atctgatttt gcaaactttg
    22561 ctcaatttgg ctagcagcat agatagcgag atttcctccc ttggaatgcc cagctagaat
    22621 aaccttttgc ttaggatgat gggcaaaaaa gttctttaaa tagcgaaggg cgtgcttttg
    22681 agcaggaatt tccttcatat aggtcaggtg gaaatcttcc ttccagccaa tgatactgtc
    22741 atctgtccca cgaaagacaa tcagataggt atcgaggctg acacgataag tcatagccgc
    22801 aaattgcttt tgcagttcag ggtcgatgtc gttgataaaa tgggagagtt tgcaattttt
    22861 gaagcgcttg tgttgagcca attcatctaa taattgaagg cgatttttgc tagtaagcat
    22921 ggtgggatct cttggaacct gaggtgctag atctaaaaga cgttgaggaa gtgtggagac
    22981 cagattatca aaggagaggt aggtgatttc tattaaggtt agaatgtcta actcattcaa
    23041 gggaaggtcg taataagaat catatgcgac atctttcaga tagtcaaaaa tattggccat
    23101 aagagctcct ttctttttat ttatcttagc aaatttccct caaattagct agtagcattg
    23161 cctgtttgta ctggctaaaa acaggctatt tcaaattcag tttcagacca tctagcatgg
    23221 aaaaatctgt tataatagta gaaaaggaga agcgcatgca caagatttta ttagtggaag
    23281 atgatcaggt cattcgtcaa caggtcggga aaatgctctc tgaatgggga tttgaagtgg
    23341 tcctggtaga agactttatg gaagttttga gtctatttgt tcagtcggaa cctcatctgg
    23401 tcctcatgga tattggtttg cccttgttta atggttatca ctggtgtcag gaaatccgca
    23461 agatttccaa ggtacctatc atgtttcttt cttcgagaga ccaggctatg gatattgtca
    23521 tggcaatcaa tatgggggca gatgactttg tgaccaagcc ttttgaccag caggttcttt
    23581 tagccaaggt tcagggcttg ttgcgtcgtt cctatgagtt tgggcgtgat gagagtttgc
    23641 tggaatatgc tggtgttatc ctcaatacca aatccatgga tttacattat caagggcaag
    23701 tcttgaattt gaccaagaat gaattccaga ttttacgcgt gttatttgag catgcaggca
    23761 acatcgtagc acgtgacaac ctgatgcggg aactttggaa cagtgacttt ttcattgatg
    23821 ataataccct ctctgtcaat gtggctcgtt tgcgtaaaaa gttggaggag cagggattgg
    23881 taggatttat cgagaccaag aaaggaatag ggtacggatt gaagcatgct tgattggaaa
    23941 caattttttc tagcctatct gcgctcccgt agtcgtcttt ttgtctatct gctttctttg
    24001 gcatttcttg tcttactctt tcagttttta tttgccagtc taggaattta cttcctctac
    24061 tttttcttct tgtgttgctt tgtaaccatc ttatttttca cttgggacat attggtggaa
    24121 acgcaggtct atcgccagga acttctctat ggagagaggg aagccaagtc tcctttggaa
    24181 atagctttag cagaaaaatt agaagcgcgt gagatggaac tctatcagca gaggtcaaaa
    24241 gcagaaagaa aactgacgga tttgctggat tactatacct tgtgggtcca tcagataaag
    24301 acccccattg cagccagtca actcttagtt gcagaagtgg tcgaccgcca actgaagcag
    24361 cagctagaac aggaaatttt caaaatcgac tcctatacca acctagtttt acagtacctg
    24421 cgtttagaaa gtttccatga tgatttgctc ttaaagcagg ttcaaattga ggacttggtc
    24481 aaggaaataa ttcgtaaata tgctcttttc tttattcaaa aaggcttaaa tgtcaatcta
    24541 catgaccttg ataaagaaat cgtgacggat aaaaagtggc tgctagtggt tattgagcaa
    24601 atcatctcaa acagtctcaa gtacaccaag gaaggtggtc tggagattta tatggatgac
    24661 caagagcttt gtatcaaaga tacgggaatc gggataaaaa acagtgatgt cctccgagta
    24721 tttgaacgtg gcttttcagg atacaatggc cgtttgaccc agcagtcctc tggacttggc
    24781 ctttatctat ctaagaaaat ttctgaagaa ctggggcacc agattcgtat cgagtctgag
    24841 gtcggaaaag gaacgacagt gcggattcag tttgctcaag tgaacttagt ccttgagtaa
    24901 aaatagatga gaaaaagcct ccagattgga ggcttttatg ctagactctt atttctcaac
    24961 gcgtgatttg ttggcgatat cagctaggat agcttgaaca aaattatcaa ctgagacagt
    25021 ttgtgtttct ttttggccgt agcgacgaac gttgactgtt tcgtcttcca tttctttgtc
    25081 tccaacaatt aattggtaag gaatcttgct ggtttgtgaa gcacgaatct tgaactgcat
    25141 tttttcattg cgctcatcta cgtctgcacg gacaccgcgg tcacggagtt tcttggccac
    25201 ttcccaagcg tagtccacgt gtttttcgtt agatactggg atgagggtta cttggtgtgg
    25261 tgccagccat gttgggaagg cccccttgta gttctcaatc aagatagctg tgaagcgttc
    25321 catagttgag ataaccccac ggtggatcat gactggacgg tgatcttcgc catcagctcc
    25381 gatgtatttg aggtcgaagc gttctggcaa caagaaatca agttggatag tagaaagggt
    25441 ttcttctttt ccaagggcag ttttaatctg gatatccaat tttggtccgt agaaggctgc
    25501 ttcaccttcg gcttcaaagt agtccacgcc catttcatca agagctgcac gaagcatggt
    25561 ttgggcattt tcccacatct catcgttatc aaagtactta tgagtatctt gagggtcacg
    25621 aagagagagg cggaagcggt agtcagtcaa gttgaagtct tcataaacat cgataatcaa
    25681 ctgaagggca cgttggaatt cttcttggat ttgttctgga gtaacgaata ggtgaccgtc
    25741 gttgagtgac atttcacgta cacgttgaag gccagtgagg gcaccagatt tttcgtaacg
    25801 gtgcatcata ccgatttcag cgatacggat tggcaattca cggtaagagt gaacatggtg
    25861 tttaaaaact tggatgtggt gcggacagtt cattggacga aggacaaatt cttccccgtc
    25921 acccatgtcc atggttggga acatgtcttc ttggtaatga tcccagtgac cagaagtctt
    25981 gtaaagctca acagaagcaa gtggtggagt gtagacgtgt tggtagccag aagccaactc
    26041 tttgtttacg atgtagcgtt ccaattcacg acggatagtc gcaccatttg gtaaccagaa
    26101 tggcaaacct tgtcccactt cttgtgaaat cataaagagg tcaagctctt taccaagttt
    26161 acggtggtca cgttccttag cttcttcacg catttgaagg tagtttttca agtctttctt
    26221 gtcaaaccaa gctgtaccgt agatacgttg catcatagcg ttgtcgctgt ttccacgcca
    26281 gtacgcacca gctacatgga gaaggtggaa gatttggata cgacctgttg atggaacgtg
    26341 aggtccacgg cagaggtcta catattcacc ctgacgatag atagtcaaac cgccttcgtc
    26401 ttctgagtgt tcttcaatca attccaactt gtaagggtca tttttgaaga tttcatgtgc
    26461 ctcgtcttta gtcacttctt cacgaataga tgggaagttt tctttgacga ttttttgcat
    26521 ttcttcttcg atacgaggaa ggtcttcgtt agagatttga ccagctgtgt tgtcagtatc
    26581 gtagtagaaa ccatcttcga tggctggacc aactcccaag tgaatgtctg ggaaaagacg
    26641 acgagctgct tgggcgaaca agtgagctgc tgagtgacgc aagattggaa gggcatcttc
    26701 gtgatcaggt gtcacaattt cgatgcttcc atcttcagtg atagcgcgag tagtgtcgat
    26761 gagtttgccg ttgaatttac cagccaaggc ttttttagct agggaattgc tgatagattg
    26821 ggcaatttca aaagttgtta cgccagattc gaattcacga acagcgccat ctgggaaagt
    26881 aatgttaatc atgatgttct ccttttttat tatttcatag tttctaagca ttgatttttt
    26941 atcagcagac tacttccgag tcctaacgtt caattgcttt tctttatcag agaggaagtt
    27001 ttccatttcc gtcagaatgt ctatatccaa acgttgagaa agttctaata gccaccagat
    27061 attttctgaa agtttttgtt ccagtgtgta gggtgtttca tcatagtagc gtccttgctt
    27121 tgtcatcacc agtcattgga aatttccaat atcattagat aaagccaaga ggtcttcttc
    27181 taccgtccac ttgaaatcat gatgcttaac ttccagttcg tgataagctt gtcggattgc
    27241 ccaactgcgc tccactaatt cttgcaactc cataattagt atcctaataa aaaaatgcga
    27301 catcctcgaa aggacgtcgc aacgtggttc caccttcatt tatgtacctc aaaagagagg
    27361 gacacctctg atcggctcta acgtggccac cgttttatgt tttcataaaa aactcaagag
    27421 tagtatcagt ttagacttcc tatgcgtttc cagcaaccac gcactctcta gtagaaaggg
    27481 actaagtgac ttgtctctat ggattattat agcatgattg tgagattttt caaggatttt
    27541 gtgaaagttt tttgtttttc attttttgta gaatatgctt gatgctaata ccagaatcca
    27601 tgccgaccag agcaactgtt tcatagatta gcaagtaaat tagtataaac tcaccaaagg
    27661 ttatgatagt aaagtaaaag agcagtccgt taccaactaa ccatcaaagt gagaaacgga
    27721 aacggtagct atagagtaac gatacaatca agattaccag aggtgtaaag aagagaagat
    27781 tgtttatata gtgtaagggg gtcagaaaca actagcaaga acaactcata aagtggccaa
    27841 taaaagaata atatgaccag atagtagggg agatgggaat agaatctacg catgataatc
    27901 acctcgtttt aaaaaaacag acaacttaga tctcttgcta tgattattat agcatattta
    27961 aaacaagaaa gtaaatttgt tgacaaagaa gatttttttc actagaatca tggttagttt
    28021 tcttggtaga aatattttga taataattaa gcaagactat tgacattgat tattaaaagt
    28081 gtcacaattg ttgtaactaa aataattaca acaagagagg tataaaaaat gaaactagca
    28141 gttattgcag caaatggaca agcggggaaa gcaattgttg aagaagcagt caaacgagga
    28201 catgaagtaa cggctattgt tcgttctgaa aacaaaagtc aagctgagtc tattattaaa
    28261 aaagatttat ttgagttgac aaaagatgat cttactggat ttgatgctgt tatttcagcg
    28321 tttggtgctt atacaccaga tacgctaccg ctccacagca aatcaatcga actatttaat
    28381 cagcttcttg ctggaacaca gacacgtttt ctagttgtag ggggagcagg aagtctttat
    28441 atagatgaaa caaaaacgac acgactttta gatacgtctg attttcctga agaatttaag
    28501 tcactagcaa aagatcaagc ggatgaattg gatcttcttc gtacaaaaaa taatctcaac
    28561 tggacttttg tcagtcctgc tgtagatttt attcccgatg gtgaaaaaac aggaaactac
    28621 attctagcag gagaaatttt tactactaat gaaaaaggaa taagccagat tagttatgca
    28681 gattatgcta ttggattggt agatgaactt gaaaaaggtc atcatataaa agaacgtatc
    28741 tctttacttg aaaaataata actatgagag tattaatgtt tgacagaaaa tttaaatctt
    28801 ggtaaaatag ggattgtcgt aaagacaaat aacttcttct tggttacagg catgccaacc
    28861 tgtcactcgg atgaagccaa ataaaaagga gaaacatcat ggcaatctca aaagagaaaa
    28921 aaaatgaaat cattgcacaa tatgcacgtc acgaaggtga tacaggttca gtagaggttc
    28981 aagttgctgt ccttacttgg gaaatcaacc accttaacga acacatcaaa caacacaaaa
    29041 aagaccacgc tacttaccgt ggattgatga aaaaaatcgg tcgccgtcgt aacttgcttg
    29101 catacttgcg taaaaacgac gttaaccgtt accgtgagtt gatcaactct ctaggacttc
    29161 gtcgctaatt caagatacaa atgctgtcaa aagcacaaag caaaaatagg aaaattgacg
    29221 aagaaacttc agtttctagg agattttatc ttttttgcca agtgcttagg ccgtgttcaa
    29281 ttgagcatat cttgataatg aagctactct aaattgggta gcttttttgc gttgtcacct
    29341 tacctcgata tactcaagta taatctttgg ttacggttcc tagcactgta aggtaaaata
    29401 aaccagcttt tctcccttcg gggagatttt ttttgtttca ctgaaagttt cgtacgatct
    29461 tcgcctcgtc gcctagtcta taaacgattt atctagcaag aatcatgatg ctaagggcgt
    29521 caaaaatccg tatgaaaata gggaaatgac acagtgttcg atgagcacaa ggagtttcat
    29581 ctttttcact gggattttag cccaggctca aatcagctct ctgacttcag agggcttttt
    29641 tattgaggtt ttatcggtaa caattttgga gactacaaaa acctcaaacg gtatcagcta
    29701 attacaccat aaaggtgcgc agttactcgg cttttcaagc cgagtagctg tatgcaagcc
    29761 ccctagaaga gcccactctt tacaaagtaa agtatagtat gttatacttt acatggaagt
    29821 agtcactgaa ttccagttag aaattacttt gtaactacgt tttgaggagg agtaaaatgc
    29881 tttcctacgt tcgacattac ccactagcga tagctaaatt aatgtgtctg tgctctccta
    29941 aaatctgctg atttattact gactaataca ggaggttttt tttatgggac agacaatcat
    30001 atctgctatt ggtgtttata tttccaccag tatcgattat ttaattattt taattatttt
    30061 atttgcacag ctatcacaga ataaacagaa atggcatatt tatgcggggc aatatctagg
    30121 cacaggctta cttgtagggg cgagtttagt tgctgcttat gtcgttaatt tcgtgcctga
    30181 agaatggatg gttggattgc ttggtttaat ccctatctat ttagggattc gctttgcaat
    30241 tgttggagaa gatgcggaag aagaagagga agaaattatt gaaagattag aacaaagcaa
    30301 ggcaaatcaa ctgttttgga cagttacatt gctgacaatt gcgtctggcg gagataattt
    30361 aggtatctat ataccttatt ttgcttcgtt agattggtca cagaccctcg tggccttgct
    30421 tgtgtttgta atcggcataa ttatcttttg cgagattagt agggtgttat cctctattcc
    30481 gttaatattc gagacaattg aaaaatacga gcgaatcatt gtgcccttag tattcattct
    30541 acttggacta tacatcatgt atgaaaatgg cacgatagag acttttctga tcgtgtagat
    30601 ttttttgttt cactaggatt ttagcctgag ctcaaatcag ctctctgatt ttcagagggc
    30661 tttttcattg agtataaatg gacaatctta aagttactct attgtagagt ggcttttgat
    30721 ttgattagga gattcttata aatatagtaa aatgaaataa gaataggaca aaatcgatca
    30781 ggacagtcaa atcgatttct aaaaatgttt tagaagtaga ggtgtactat tctagttcca
    30841 atctactata taaatgaaaa ccttagctgt ttggccaagg ttcatttctt ctatgcaagt
    30901 tcttctcttt tcttatctgg gttccagatg aagcttgcga taaagctaaa gatgatggtt
    30961 aggatagcga ggataatagc aaggatgagg gcagggatgc ggataaacca ccagagtgga
    31021 tttggttttt tatcattgtg ccattgtttc gtttcttcgt ccagacgttg gaattctgtt
    31081 tgaatacgat tggcgactgt ttcaatgcct tcatcattca ttttcttgat atctgagata
    31141 tcgattggat ttccaaagtt catatcgaca cgttcacggc taatcaagcc cttcaaagtc
    31201 atgggaccgg tgtaggtaac cggcatgata cggaccttgg ccattttggc aatcagtgct
    31261 gcgcccccct tgacatcgtt tgagtggcgg ctaccacttg gaaacatgat gagagagcgg
    31321 tcactttttt tgagaacgtt gataggatat ttgatggctg aggcgctggg attttcacgg
    31381 ttgatgggaa aggcgccaca catacgaatc caccaaccaa agatacggtt ggtaaagagt
    31441 tctttttttg ccataaagat gaactgtttt ggcttggtcg caaaggccat ataaacagga
    31501 tcccaccagg tacggtgagg cgcaactaaa atataatttt catcttgatt aggaatttta
    31561 tcagtattat gatagtgagc attgccattg atggaccata agagcaatac aactaatcca
    31621 cgcaaataag tataaaacat gcgatctcct tcgattgttt tcttgttatt attatacctt
    31681 atcaaaggag ggctggcaaa cttttccctt gactagatgc atatttggga tgagattaga
    31741 attctgttaa aaaaaatgat ataatagaat ttatggataa aaataagatt atgggattaa
    31801 cccaaagaga agtcaaggaa agacaggctg agggcttggt caatgacttt accgcatcag
    31861 ccagtaccag cacttggcaa atcgttaaac gaaatgtctt tacccttttt aacgctttga
    31921 actttgccat tgctttggcc cttgcctttg tgcaggcttg gagcaatctg gtcttctttg
    31981 ctgttatctg ctttaacgct ttttctggga ttgtgaccga gctacgagcc aaacacatgg
    32041 tggacaagct caatctcatg accaaggaaa aggtcaaaac catccgtgat ggtcaggaag
    32101 ttgctcttaa tcctgaagaa ttagtgctag gagatgtcat tcgtttgtct gcaggagagc
    32161 agattcctag tgatgccttg gttttggaag gctttgcgga agtcaatgaa gccatgttaa
    32221 cgggagaaag tgatttggtg caaaaggaag ttgacggctt acttttgtca ggaagtttcc
    32281 tagccagtgg gtcagtttta tctcaagttc accatgtcgg tgcagacaac tatgctgcaa
    32341 aactcatgct tgaggctaag accgttaaac ccatcaactc ccgtatcatg aaatcgctgg
    32401 acaagttggc tggttttact gggaagatta tcattccctt tggtctagct ctcttgctgg
    32461 aagccttgct tttaaaaggc ctgcctctca agtcatccgt tgtaaattcg tcgacagccc
    32521 ttttgggaat gttgcctaag ggaattgccc ttttgaccat tacttcgctc ttgactgcag
    32581 tgattaagct tggcttgaaa aaggtcttgg tgcaggagat gtactctgtt gagaccttgg
    32641 cgcgcgtgga tatgctctgt ctggacaaga cgggtaccat cacccaagga aagatgcagg
    32701 tggaggctgt tcttccactg acggaaactt atggtgaaga ggctgttgcc agcatcctga
    32761 ccagctatat ggcccatagt gaggataaaa atccaacagc tcacgccatt cgccagagat
    32821 tccaaggtca agtagtctat cctatgctct ctaatcttcc cttctccagc gaccgcaagt
    32881 ggggagccat ggagttagaa ggtttgggaa tagttttctt aggggcacct gagatgttgc
    32941 tggattctga ggtcccagaa gccagagagg ccttggagag aggttcacgt gtcttggtct
    33001 tagctctcag tcaggagaaa ttagaccatc acaaaccaca gaaaccatct gatattcagg
    33061 ctctagcctt gctggaaatc ttggatccta ttcgagaggg agcggcagag acgctgaact
    33121 atctccgttc tcaggaagtg gggctcaaga ttatctctgg tgacaatcca gttacggtat
    33181 ccagcattgc ccagaaggct ggttttgcgg actatcacag ctatgtcgat tgttcaaaaa
    33241 tcacggatga ggaattgatt gctctggcgg aggagacagc gattttcgga cgtgtttccc
    33301 ctcatcaaaa gaaactcatt atccaaacgc tgaaaaaagc gggtcataca acagctatga
    33361 ctggggatgg agttaatgat atcctggctc ttcgtgaggc ggattgttct atcgtaatgg
    33421 cggagggaga cccggcaacc cgtcagattg ccaacctggt tctcttgaac tcagacttta
    33481 atgatgttcc tgagattctt tttgaaggtc gtcgtgtggt caataacatt gcccacatcg
    33541 ctccgatttt cttgataaag accatctact cattcttgct cgcagttatc tgcatcgcca
    33601 gtgctttact aggtcggtct gaatggatct tgattttccc cttcattcca atccagatta
    33661 ccatgattga ccagtttgtg gaaggtttcc caccattcgt tttgactttt gagcgaaata
    33721 tcaaacctgt cgagccaaac ttcctcagaa gatccatgct tcgtgcccta ccaagtgccc
    33781 tcatggtcgt gtttagcgtt ctttttgtga aaatctttgg aacgagtcaa ggttggtctg
    33841 agttagaaat ctcaactctc ctctattatc tcttggggtc aattggtttc ttatccgtat
    33901 ttagagcctg catgccattc actctatggc gagtcctatt gattgtctgg tcagtaggag
    33961 gcttcctagc cacagctctc ttcccaagaa ttcaaaaact gcttgaaatt gcaaccttaa
    34021 caggacaaac attacctgtt tatggtgtca tgatgttggt ctttaccgtg attttcatcc
    34081 tgaccagtcg ctatcaagcc agaaaataaa gaaaggctgc aatctgtgga ttgcagtttt
    34141 ttatacgatg gatctatcgt agatctgatg tgcaaggcct acgtgtctat tatctatcgg
    34201 tgaacccaag agtgattctc aagcctgctt ggattgaggt aatagattca aatatctgta
    34261 gttagactat ttgaagtttg atgtaagaaa gagaaagcga cagattgaag taattttaac
    34321 tctcttctat tgctagaaca aatggtcgga taggttggta gtttgaaaat gaagatgcaa
    34381 tctattgtta aatggaacat agtgttattt attagaaaat cgtttggttt atttcttatc
    34441 aaatacgaaa agcaacttaa atatttcaac taaaatagat gttatgaaga aaaggtaaaa
    34501 tgattttggc atagtgaggt tctgttctat ttgatatcat atttttgata aaaacaaaaa
    34561 tgtccattgc aaaggacaaa atgcgaagta tattattttt tgaaagcgat ataatggaat
    34621 cataaaggag gtgtatcgtg tctagaaaac aagaacaaat ggaaacgttg ttgctccttt
    34681 tgcgagatag taaggattat atatctgcta aagtattggg agaaaaatta aattgctctg
    34741 ataaaacggt ttatcgcctt gtcaagggaa tcaacaaaga ttgtccggta gaagcattca
    34801 ttttatctga aaaaggcaga ggtttcaaat taaatccaag aagttccctc gtggacgttg
    34861 atgggaattt tacagaggct tttgatcctg aagtaaggcg tgaaaaatta ctagaacgtc
    34921 tcttgttgac tgctcctaag ccacattcta tttatgattt aggagaggaa ttctacgtaa
    34981 gcgagtcagt agtactaaaa gatcgtcaga tattacaaga gagtctagca atttatgggt
    35041 tagatttaaa aatgagacaa cgaaagcttt ttattgatgg ggatgaggct caaattcgtt
    35101 cagccattct aaatctactg ccaatgttta atcagttgga tttagagcaa attacacaga
    35161 ataaggttca gcctcttgac ggagaacttg ctcacttttg tttgggatta ctgattacac
    35221 ttgagagaga attgggggta aacattccct atccatataa tataaatatt ttctctcacc
    35281 tgtatatttt tatcagtagg aatcgtcgta gtactagtat tcatgttgta gcaccttcaa
    35341 aacctactat tgttgatgag aaaatttaca gtgtctgtca aaaaattatt caagaaattg
    35401 aacaatattt taggatgaag gttgatgcag ttgagattga ctatctttat caatacgttg
    35461 tatcttcgag attgcaaaaa ccattttctt ccgggaagct tcctttttct cagcgagttt
    35521 tagatgtcac tcattactat tttagccgta tgtgtatgga caatagagag attgaaacga
    35581 cagatcctga ctttgttgac ttggcaagtc atatcagtcc cttactgagg agattagata
    35641 atagagtaca gattaagaat agtcttttat cacaaattct tttaacctat cctaatctgg
    35701 ttaaagagtt aacaactatt tctaaagaag tgagtctagt atttggtttt gcgtccttga
    35761 gtctggacga gattggtttt ctagtcttat attttgcacg gtttcaagaa aagcgagcac
    35821 gtcctctaaa aacagtagtg atgtgtacat caggtgtcgg aacttcagag cttttacgag
    35881 cacgattaga aaagcaattt actgaattgg atattattga tgtagttgct tatcatcaat
    35941 tagatgagct gataaatcta tatccagatt tagatttcat tgtgacgacg gtagctttgc
    36001 aggaaccagc aagtgtcccg tttgtcctag ttagtgtttt tctaaccgag ggtgataaac
    36061 aacgtcttca agcaaaaatt caggagataa actatgaata atctttcgct tgtccttatg
    36121 gatatatctg ttcaaaatcg tcaagaagcc tacaaagaat tagcaaatca aatcagcctt
    36181 cttgtttctg aagatacaga aaaaatagaa gagcttctat attaccgtga gagacaggga
    36241 agtatagagg ttgctaaagg tgttcttcta ccacattgtg aaggaaactt tcaacatcat
    36301 gtcttagtga ttactagatt aaaaccacct atcagagaat ggtcgaagga tatccagtgt
    36361 gttgacctta ttatcggttt ggccattgca gtatcacagg acaagtcatg tattaaaaca
    36421 ttgatgagaa gactagcaga tgaatcattc ataaatcaat taaaacagtt aacaaaagaa
    36481 gaattacggg agataatata tggaaatcaa agatattctt aatgtgagtc tgatccagac
    36541 ggatttacag atgcagagca aagaagaggt ttttgaggca ttagctcaac tattggttga
    36601 gacgggttat gtgtctgata gagaccaatt tatcgaaggt ctttatcaga gagaggcaga
    36661 aggacagact ggtattggga attatattgc tattccccat agcaagagtt ctgctgtgga
    36721 gaaggcgggg gtagtcatag ctataaatca caatgagatt ccttgggaga ccattgatgg
    36781 gaaaggggtc aaagtaattg tactctttgc agttggtgat gatacagaag ctgctaggga
    36841 gcatttgaag accttatcac tctttgctag aaaacttggt aatgacgaag ttgttgccaa
    36901 attagttcgg gctcagacat ctgatgatgt gattgcagct ttttgttaat aagaaaaaat
    36961 tttggagggt atccgtatga aaattgttgg tgttgcagct tgtactgtgg gaattgccca
    37021 cacttatatt gcacaggaaa aattagagaa tgccgcaaag gtagctggac atgtgattca
    37081 tgttgagact caggggacaa taggggtaga aaatgaattg agtcaagagc agattgatgc
    37141 agcggatgta gttattttag cagttgatgt taagatttct ggtatggaac gctttgaggg
    37201 taaaaagatt atcaaggttc caacagaagt ggcagtcaaa tctcccaata aactgattgc
    37261 taaagctgtt gagattgtta cgaaataact gaaaatattt aaggagaaaa tatatgttga
    37321 aacacttaaa cttaaaaggt cacttattga cagccatttc ctatatgatt ccaattgttt
    37381 gtggcgcagg attcttagtt gccattggtt tagcaatggg gggtggtgtt cctgacgctc
    37441 ttgtagcagg aaaattcact atctgggatg ctttagcaac tatgggtggt aaagcccttg
    37501 gtctcttgcc agttgttatt gctacaggtt tgtcttactc gattgctggt aaaccaggga
    37561 ttgcaccagg ttttgttgtt ggtctaattg ccaattctgt tggttcaggg tttatcggtg
    37621 gtatcttggg aggttatata gctggtttct tggttcaagc gattattaaa aaggtcaaag
    37681 taccaaactg gattaaaggt ttaatgccaa ccttgattat tccttttgta gcctctttgg
    37741 taagtagttt gattatgatt tatattattg gagcgcctat cgcagccttt accaactggt
    37801 tgacgagctt attacaaagc ttgggaagtg cttcaaatgg tttgatgggg gcagttattg
    37861 gagttctcag tgctgttgac tttggtggcc cacttaataa aacagtctat gcctttgtgt
    37921 tgactttaca ggctgaaggt gtgaaagaac cattgactgc tttgcaattg gtgaatactg
    37981 ctacaccagt tggatttgga ttggcctatt ttatcgcgaa attactcaaa aaaaatatct
    38041 atactcaaga ggaaatcgaa acattgaaat cggctgttcc tatggggatt gtcaatattg
    38101 ttgaaggtgt aattccgatt gttatgaata acttggttcc aggtctcatt gcaacaggta
    38161 tcggtggtgc tgttggtggt gctgtttctt tgacaatggg tgctgattct gccgtgccat
    38221 ttggtggagt gcttatgtta ccaaccatga ctcgtccagt agctggtatt tgtgctttgt
    38281 tagccaacat tgtagtcaca ggacttgtct acgcgatttt gaaaaaacca ataaaacata
    38341 cagaaccagt tatgactgtt gaagaagaga ttgatttgtc agatattgaa attttgtaag
    38401 agggtaacga tgtcaagaat tgaattttca ccatctttga tgaccatgga tttggacaaa
    38461 ttcaaagagc agattacttt tttgaatgat aaagtagcat cttatcatat cgatattatg
    38521 gatggccatt ttgttcccaa tattaccttg tctccttggt tcattcaaga agttcaaaaa
    38581 attagtgaca cacctttatc agttcatctg atggtcacag acccaacctt ttgggtagat
    38641 caagttctcg atttacaatg tgagtatatt tgtattcatg ctgaagttct gaatggtctt
    38701 gcttttcgtt tgattgataa aattcatgat gcaggtctaa aggctggtgt tgtccttaat
    38761 cctgaaacac ctgtttctac aatctttccc tacattgatt tacttgacaa agtaactatt
    38821 atgactgtag atccaggttt tgcaggacaa cgctttttgg agtctacctt gtataaaatc
    38881 caagaactcc gtcagcttag agttcagaat ggttatcact acatcattga gatggatggt
    38941 tcttcgagtc gtaagacttt caaacaaatt gatgtggcag gaccagatat ttatgttata
    39001 ggtcgcagtg gattatttgg tttggatgac gatattgcca aagcctggga tatctgttct
    39061 agagattacg aagaaatgac cggaaaaaca atgccaatca aataatggtt tgagaagaaa
    39121 tttattagtt aggaggaata tatgtcacta caatcagtta acgccattcg ttttcttggc
    39181 gtagatgcta ttaacaaatc taattctggt cacccgggaa tcgtcatggg tgctgcgcca
    39241 atggcttata gcctatttac aaagcacctt agaattacac ctgagcagcc aaactggatt
    39301 aaccgagatc gctttatctt gtctgcgggt catggatcaa tgctactgta tgctctcttg
    39361 catttaacag ggtataacga tgtatccatg gacgagatta aaaatttccg gcaatgggga
    39421 tctaagacac ctggtcatcc tgaagtgacg catacgtctg gtgtggatgc gacatctggt
    39481 ccgcttggtc aggggatttc tactgccgtt ggtttcgccc aagcagagcg ttttttagct
    39541 gctaagtaca acaaagatgg ttttcctatt tttgaccatt atacttatgt tatcgctgga
    39601 gacggtgact tcatggaagg agtgtctgcg gaggcggctt cttatgcagg tcatcaagct
    39661 ttagataagc ttatcgtcct ctacgactcc aacgatatct gcttggatgg tgagaccaaa
    39721 gatactttct ctgaaaatgt tcgcgcccgt tacgatgctt atggttggca tacagttctg
    39781 gtagaagatg gaacagattt agcagcaatt tctacagcaa ttgagacggc caagttttct
    39841 ggtaagccga gtttgattga agtgaaaacg gtaattggtt acggctcacc caataaaagt
    39901 ggtacaaatg ctgttcatgg tgcaccacta ggagcagaag aaacaggagc aactcgtaag
    39961 tttttgggat gggattacga tccatttgaa gtaccagagg aagtatattc tgatttcaag
    40021 acaaatgtag cggatcgtgg tcaggaggca tacgatgctt gggctagttt ggtgtctgat
    40081 tacaaggttg cttatcccga agttgctagt gagattgacg ctattgtagc tggaaaatcc
    40141 cctgtaacca ttactgaaaa agacttccct gtctatgaga atggcttctc tcaagcaact
    40201 cgtaattcgt cccaagatgc tattaataca gcagcagcag ttttaccaac cttcttaggt
    40261 ggatcggcag acttagctca ctccaacatg acctacatca aggcagatgg cttacaagat
    40321 aaatataatc cattaaaccg caatattcag tttggggtac gtgaatttgc catgggaaca
    40381 atcctcaatg gaatggctct tcatggtggt ttacgagttt atggcggaac cttctttgtt
    40441 ttctctgact acgtcaaagc tgctattcgg ctatcagcca ttcaggagtt gcctgtaact
    40501 tatgtcttta cccatgattc aattgccgtt ggtgaagatg gtccaactca tgaaccagtt
    40561 gaacatttgg caggtttacg ctcaatgcca aacttgactg ttatccgtcc agcggatgcc
    40621 cgtgaaactc aagcggcttg gcatcatgcc ttgaccagta ccaccactcc aactgtcatt
    40681 gtcttaaccc gtcaaaactt ggtagttgaa gaagggacag actttggtaa ggtcgctaaa
    40741 ggagcctacg tcgtgtatga taccccggga tttgatacta ttatcattgc tacaggatct
    40801 gaggtcaatc tagctatcaa agctgctaag gaattggttt tacaaggtgg taaagtacgt
    40861 gtggtatcta tgccctcaac cgaactattt gatgctcaag atgctaccta caaggaagac
    40921 attttaccat ctaagactcg tcgtcgtgtg gccattgaaa tggcagcgac ccaaagttgg
    40981 tacaagtatg ttggtttgga tggcgcggtc atcggtattg acatcttcgg tgcgtctgcc
    41041 ccagctcaga ctgtgattga taattatgga tttacggtag agaatatcgt tgctcaagtt
    41101 aagtccctat agaaaccaat tacaatgaag atacagctgt tgtcagacta gcagatgtag
    41161 tgatagacac taatcagatg attggttatt taaaaactat aatgagaatg taataattta
    41221 tctacgaaag ttatagtaga tagtatacac aatagagtat accctgaaac ggttgcgaag
    41281 tacgctaatc actttgctac tgatctagat agtttcttta atcaataaac acagcatcca
    41341 cagattgact taggatattg taagtttttt gaaagctaga gagaaggtct ataaaattca
    41401 aaaaacgcat catatcaggt gtacatgaac tgaccccaaa agttagacag aaaaaatcta
    41461 actttttggg gtgttttatt atgaaattaa gttatgatga taaagttcag atctatgaac
    41521 ttagaaaaca aggatatagc ttagagaagc tttcaaataa atttgggata aacaattcta
    41581 atcttaggta catgattaaa ttgattgatc gttacggaat agagttcgtc aaaaaaggaa
    41641 aaaatcgtta ctattctcct gatttaaaac aagaaatgat tcataaagtc tgacatgaag
    41701 gctggactga agatagagtt tctcttgaat acggtctccc aagtcgtacg atacttctta
    41761 actggctagc acaatacagg aaaaacgggt atactattgt tgagaaaaca agagggagag
    41821 tacctaaaat gggacgtaag ccaaaaaaga gacctgaaga gaggacagaa ttagaacgtc
    41881 ttcaatcaga aaatgagtac ctgagagcgg agaatgccat cctaaaaaag ttaagagaac
    41941 tccgattgaa ggaggaaaaa gagaaagaag aaagacagaa attgttcaag aattaatgac
    42001 tgagttttcg ttagatcttc ttctaaaatc cattaaacta gctcgttgga cctactacta
    42061 tcacttgaaa cagctagata aa
//
LOCUS       DBERMR010000012        41451 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.012, whole genome shotgun sequence.
ACCESSION   DBERMR010000012 DBERMR010000000
VERSION     DBERMR010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 41451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41451
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.012"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(34..228)
                     /locus_tag="ACL8A1_000700"
     CDS             complement(34..228)
                     /locus_tag="ACL8A1_000700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201508.1"
                     /translation="MKIKEQTRKLAAGCSKHRFEVVDRTDEVSSKHCFEVVDRTDEVS
                     NHTYGKVKLTWFEESFEEYK"
     gene            299..955
                     /locus_tag="ACL8A1_000701"
                     /pseudo
     CDS             299..955
                     /locus_tag="ACL8A1_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
     gene            945..1268
                     /locus_tag="ACL8A1_000702"
     CDS             945..1268
                     /locus_tag="ACL8A1_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HGS1201509.1"
                     /translation="MVSKYLLLAVIFSGLVTWILRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAI
                     LRLVF"
     gene            1372..2202
                     /locus_tag="ACL8A1_000703"
     CDS             1372..2202
                     /locus_tag="ACL8A1_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS1201510.1"
                     /translation="MKKIVKYSSLAALALVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPKPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            2356..3210
                     /locus_tag="ACL8A1_000704"
     CDS             2356..3210
                     /locus_tag="ACL8A1_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HGS1201511.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIGDTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEETS
                     DGLDQPVW"
     gene            3310..4683
                     /locus_tag="ACL8A1_000705"
     CDS             3310..4683
                     /locus_tag="ACL8A1_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HGS1201512.1"
                     /translation="MVFPSEQEQIEKFEKDHVVQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEAPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDVKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            4676..5737
                     /locus_tag="ACL8A1_000706"
     CDS             4676..5737
                     /locus_tag="ACL8A1_000706"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HGS1201513.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGGQ"
     gene            5739..6431
                     /locus_tag="ACL8A1_000707"
     CDS             5739..6431
                     /locus_tag="ACL8A1_000707"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HGS1201514.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(6461..7030)
                     /locus_tag="ACL8A1_000708"
     CDS             complement(6461..7030)
                     /locus_tag="ACL8A1_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HGS1201515.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADGVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPRTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            7165..7779
                     /locus_tag="ACL8A1_000709"
     CDS             7165..7779
                     /locus_tag="ACL8A1_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HGS1201516.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFISSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            7757..9427
                     /locus_tag="ACL8A1_000710"
     CDS             7757..9427
                     /locus_tag="ACL8A1_000710"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGS1201517.1"
                     /translation="MKNWRQNRMKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSR
                     EKEAQRVAQRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQ
                     LERKASPYISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDF
                     KPAGNSFAIPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIF
                     HIGETVDRESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLT
                     LRQTFANYQKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNI
                     HDIYQLELSQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLR
                     NNISDERETLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVE
                     NYFAHGVDHRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRYFEHQ
                     ASYSDQRQSIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            9439..10725
                     /locus_tag="ACL8A1_000711"
     CDS             9439..10725
                     /locus_tag="ACL8A1_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGS1201518.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFAIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLSSFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            10777..11310
                     /locus_tag="ACL8A1_000712"
     CDS             10777..11310
                     /locus_tag="ACL8A1_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1201519.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELA"
     gene            11388..11858
                     /gene="nrdI"
                     /locus_tag="ACL8A1_000713"
     CDS             11388..11858
                     /gene="nrdI"
                     /locus_tag="ACL8A1_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HGS1201520.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKRIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(12151..13413)
                     /locus_tag="ACL8A1_000714"
     CDS             complement(12151..13413)
                     /locus_tag="ACL8A1_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HGS1201521.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
     gene            complement(13723..14181)
                     /locus_tag="ACL8A1_000715"
     CDS             complement(13723..14181)
                     /locus_tag="ACL8A1_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HGS1201522.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWAAIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(14178..14618)
                     /locus_tag="ACL8A1_000716"
     CDS             complement(14178..14618)
                     /locus_tag="ACL8A1_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HGS1201523.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            14974..16923
                     /gene="mutL"
                     /locus_tag="ACL8A1_000717"
     CDS             14974..16923
                     /gene="mutL"
                     /locus_tag="ACL8A1_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HGS1201524.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(17248..17715)
                     /gene="ribE"
                     /locus_tag="ACL8A1_000718"
     CDS             complement(17248..17715)
                     /gene="ribE"
                     /locus_tag="ACL8A1_000718"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HGS1201525.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(17716..18921)
                     /locus_tag="ACL8A1_000719"
     CDS             complement(17716..18921)
                     /locus_tag="ACL8A1_000719"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HGS1201526.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(18941..19576)
                     /gene="ribE"
                     /locus_tag="ACL8A1_000720"
     CDS             complement(18941..19576)
                     /gene="ribE"
                     /locus_tag="ACL8A1_000720"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HGS1201527.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(19561..20661)
                     /gene="ribD"
                     /locus_tag="ACL8A1_000721"
     CDS             complement(19561..20661)
                     /gene="ribD"
                     /locus_tag="ACL8A1_000721"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HGS1201528.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHYVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(20796..20910)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            21067..21660
                     /gene="ruvA"
                     /locus_tag="ACL8A1_000722"
     CDS             21067..21660
                     /gene="ruvA"
                     /locus_tag="ACL8A1_000722"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HGS1201529.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDGLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            21670..22233
                     /locus_tag="ACL8A1_000723"
     CDS             21670..22233
                     /locus_tag="ACL8A1_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HGS1201530.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTETELEAMLENPAIIRNRAKLFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            22230..22907
                     /locus_tag="ACL8A1_000724"
     CDS             22230..22907
                     /locus_tag="ACL8A1_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HGS1201531.1"
                     /translation="MMSNKNKEILIFAILYTVLFMFDGVKLLASLMPSAIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVRL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(23062..24093)
                     /locus_tag="ACL8A1_000725"
     CDS             complement(23062..24093)
                     /locus_tag="ACL8A1_000725"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HGS1201532.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCPKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVIRFDYNK"
     gene            complement(24348..25031)
                     /locus_tag="ACL8A1_000726"
     CDS             complement(24348..25031)
                     /locus_tag="ACL8A1_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HGS1201533.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(25043..25987)
                     /locus_tag="ACL8A1_000727"
     CDS             complement(25043..25987)
                     /locus_tag="ACL8A1_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HGS1201534.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            complement(26148..27314)
                     /locus_tag="ACL8A1_000728"
     CDS             complement(26148..27314)
                     /locus_tag="ACL8A1_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGS1201535.1"
                     /translation="MKITEVIKKDGSKVYRANIYLGVDQVTGKKVKTKVTGRTQKEVK
                     QKATQEKIAFQKAGSTRQKASTIKNYQELANLWLESYKNTVKPNTQDNVRKLIDNHIL
                     PIFGAYKLDKLTTPLIQSIINELADKTNRGEKGAFLHYDKIHALNKRILQYGVTMQAI
                     PSNPARDVVLPRNTQKAKRKKVKHFENQELKKFLAYLDNLDSDRYRYYYETTLYKFLL
                     ATGCRINEALALSWSDIDLDNAVVHVTKTLNRDLEINSPKSKASYRDIDIDQATVSML
                     KQYKLRQTKEAWKIGQRESVVFSDFIHEYPSSSRLKRRLQTHFKRADVPNIGFHGFRH
                     THASLLLNSGIPYKELQYRLGHSTLSMTMDIYSHLSKENAKKAVSFYETALKAL"
     gene            complement(27647..28636)
                     /locus_tag="ACL8A1_000729"
     CDS             complement(27647..28636)
                     /locus_tag="ACL8A1_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3644 domain-containing protein"
                     /protein_id="HGS1201536.1"
                     /translation="MEDLSKRLVNKSIEAFIMGIEIYNKPTIKYRIEGFSFFICNAWE
                     LMLKAYIINNDGEESIYFKDSKDRTISLENAVETVFPDKHGSLRKNLIRIIELRNTST
                     HFITEDYEHIYAPLFQACVSNYILKMQEFHNEDITKQIAQNFLTLSVRIDQLNQEEIR
                     AKYSPKMAERILAEQDKIESEISTNNSDYAVPVETRFYITKRKQDADLFVKLDNTAET
                     SIGIVREIKDPNAIYPLTTREVIKIVNRYLKSKGILLTKIVGGEKQKKTFTTNDLQLF
                     NTFYDIKTNQRYCYHYKIGTRYGYSQILCDFIIEEIERNPETFVENLKKRQKK"
     gene            complement(28724..29359)
                     /locus_tag="ACL8A1_000730"
     CDS             complement(28724..29359)
                     /locus_tag="ACL8A1_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS1201537.1"
                     /translation="MAKNSPQDIKNRLYFSARLNRLMKEQGKRQIDLHNDLGIPKSTL
                     TGYVKGRSMPNTENLQKLADYFKVEASDIDPRYFVIDPDVILKYEQPVIDFVTMFESL
                     SERKQKKFFEGVIKDLEHDNLPHDFLKEPSDLVTHLGSQLDEKLRIDKELKMLEKENA
                     MLEEEIRILKISNEYALIDPKDTEAINNFRNSHSEEDIIKVEELLNKKKQP"
     gene            29512..29730
                     /locus_tag="ACL8A1_000731"
     CDS             29512..29730
                     /locus_tag="ACL8A1_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS1201538.1"
                     /translation="MEQITLKGARVSAGYTLREVGLKINRSFQTVAKYEKDSTKIPID
                     LLRELTSLYNISPDLIFLGKSTKKNELI"
     gene            29744..30370
                     /locus_tag="ACL8A1_000732"
     CDS             29744..30370
                     /locus_tag="ACL8A1_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HGS1201539.1"
                     /translation="MELVYMDGKKEPYTLSSIIAECAEVKHRHLKILLNKHREDFESF
                     GKVQFKISPSESGQNVRDYILNEQQATLLITYLRNTEPVKEFKKNLVKAFFEMREELS
                     KFRMQRALEKPKRKTLHDSIENWKQAPKHAHSTMNNLLLKAVTDRNAKQLREERGGYN
                     GIDSLTSDELEQYQAFEDMVIAMISLNMSYQEIKAMAFRNKKTRQRSA"
     gene            30805..31257
                     /locus_tag="ACL8A1_000733"
     CDS             30805..31257
                     /locus_tag="ACL8A1_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201540.1"
                     /translation="MTETTYDIIAKSLDRISMELHQADENNDFLRIGLLSGQLKAIKE
                     NLHRLLWIELPELNESHKIEAVSKSTTGMYFHPGIFEMDAMRQAFFKRQAKHFFDNEA
                     EQQAYIEHAEKEYLEATITLKDILFNFKNANRKVSKDHLIEKFEEAMK"
     gene            31254..31469
                     /locus_tag="ACL8A1_000734"
     CDS             31254..31469
                     /locus_tag="ACL8A1_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201541.1"
                     /translation="MNELDLTNTQALIFMVVLIGLLLYLNHRDRKKSAKIERESQQTI
                     ETPSEDLNPYYGRYIQLAGVRVYGGTE"
     gene            31466..31807
                     /locus_tag="ACL8A1_000735"
     CDS             31466..31807
                     /locus_tag="ACL8A1_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGS1201542.1"
                     /translation="MSLSENDKRVLRLIKVGAENSITGAEISLITKLAERTVQDIISR
                     LITRNSIPIVGVRHGTFRGYFIPANKEELLDSAKTFYNQIREEEKRLVVLMNSDLESY
                     KETLKEVGGYV"
     gene            31800..32081
                     /locus_tag="ACL8A1_000736"
     CDS             31800..32081
                     /locus_tag="ACL8A1_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HGS1201543.1"
                     /translation="MFSLSRESEQDLAHGIIEVVERYLEARDTVKPRLTGLISAQEVM
                     GELDIKYLTLQKWEKAGLRRYHPPLEDTRKVYYKITDIWKFMGVENGKN"
     gene            32068..32865
                     /locus_tag="ACL8A1_000737"
     CDS             32068..32865
                     /locus_tag="ACL8A1_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HGS1201544.1"
                     /translation="MAKTKIYFWLKIDKKFFDNIFIKRLKTVPGGYTMTVIYIRLMLE
                     SLESDCILYYEGYFDNLKEELALKLDVSEDDIDMTMAYFTKCGLIQVDEDKNAELPQA
                     KALVQQETNQAAYMREYRKQQREQKENLTMSSENLTLLTTCKTEIEKEIEKELEEEIE
                     KKTSSAPTAEISNYYQKRIGVMDGQQYQILTDYLTLDGMELEVIKIAIDKAADNGKRS
                     FSYINSILKNWRQNGIRTMVQVEDEQRLFQQKKQGQSDDDIQDPFIY"
     gene            32881..33705
                     /locus_tag="ACL8A1_000738"
     CDS             32881..33705
                     /locus_tag="ACL8A1_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGS1201545.1"
                     /translation="MELLNTDQLNEKIKVTDELCPIHQINLVQFKKPDGYTSPYCMEC
                     TRENIKQTELEGIEKALGHDLDSSTYDVLVRESTVSNELREATFSTFKAETQEEHEAK
                     EFAIKQAKQYLNGMTGNTLIMGNPGTGKSHLCYSMAKAINEGYKSRNDPKSVLFVSIT
                     EIITRIQSGWQYRQSDFTEYDALKLLTEVDYLFIDDLGTESVMNSQKNEANNWVQAFL
                     FKIFDKRDTTIINTNHNGKELARIYNDKLVSRIGKQSEGNVFIITDIKDKRMKRNF"
     gene            33837..34370
                     /locus_tag="ACL8A1_000739"
     CDS             33837..34370
                     /locus_tag="ACL8A1_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201546.1"
                     /translation="MTLKTFSDKAKTFTFTYEFKDLDTALVAGHALLGYMTGTYCQPT
                     ISLTHKDKGTLVAEYVEDNKLNKTFKRICDSFKDYYNQPVDDEAFEERYKRERVLQLK
                     ESEDFDSLLNKVTDYELELLDYADRLLSDKPIPMDSMTAFGTLEMLGNESINLLQNLD
                     VEGEYKGLADYVAEPES"
     gene            35582..36001
                     /locus_tag="ACL8A1_000740"
     CDS             35582..36001
                     /locus_tag="ACL8A1_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HGS1201547.1"
                     /translation="MTPEQVKEKLEGVKWINKEIKGLYLELEALEGGIIQKPTLSHSR
                     VQTSRENKTENNFISVLKLKEDTLQRIERLTEERMEISGLIDKLANPLERSVLRLFYL
                     NDLDIWGVAEEIGKSKASIYRAKKAAIEHLVCMVNGN"
     gene            36750..36959
                     /locus_tag="ACL8A1_000741"
     CDS             36750..36959
                     /locus_tag="ACL8A1_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201548.1"
                     /translation="MAKVKEKWNPTISHIVPKGTKLSDGTILDKETTLTQEEFTKNPP
                     VIPAGHPYYNLLARISREEIEKEDL"
     gene            37511..40342
                     /gene="uvrA"
                     /locus_tag="ACL8A1_000742"
     CDS             37511..40342
                     /gene="uvrA"
                     /locus_tag="ACL8A1_000742"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HGS1201549.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLHEGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVTGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            40335..41396
                     /locus_tag="ACL8A1_000743"
     CDS             40335..41396
                     /locus_tag="ACL8A1_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HGS1201550.1"
                     /translation="MNKRVQAFLAKIQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
BASE COUNT        12438 a         7791 c         8912 g        12310 t
ORIGIN      
        1 aatagtccat gctgtggctg gattcttttc agcctactta tactcttcga aactctcttc
       61 aaaccacgtc agcttcacct tgccgtaggt atggttactg acttcgtcag ttctatccac
      121 aacctcaaaa cagtgttttg agctgacttc atcagttcta tccacaacct caaaacggtg
      181 ttttgagcaa cctgcggcta gtttcctagt ttgctctttg attttcattg agtattatta
      241 agacaatata aggagaaaga tgaaagaaaa aggattttag gagggggcgc aggcagccat
      301 gccaacggct cttggctatg tcagtatcgg cctggcctgt gggactatcg gtgcgcccta
      361 tgtgacacct gttgagatgg gcttgatgag tctctttgtt tatgctggga gtgcccagtt
      421 tgccatgttg gcactgattg tggttcaagc tcctgtggca gctattgcta tgacggtttt
      481 tctaatcaac ttgcgtctct ttttgttgag tttacacgca tcgacttatt tccgtcatac
      541 cagtctttgg tacaatatcg gtatgtctag tatcttgaca gatgagacct atggcgtttt
      601 gatgggtgaa ttggcccata cagacaaggt aaatcctatg tggatgcacg gaaacaatct
      661 taacagctat gtggcttggt ttgtggggac agtagtcgga acggctctgg gtggcctgct
      721 accaaatcca gaaatctttg gcttggattt tgccctggtt gggatgttta ttggtatttt
      781 tgcttagcaa tttcagatta tgcaaagacg gattcctgtc cgcaatctgc tcattatcct
      841 agcagttgtt gcggtgtcct tctttttgct cttgacagtg gtgtctcagt cactagctgt
      901 tctgtttgcg acgctacttg gttgtagcat gggggtggtt ttagatggtc agtaagtatc
      961 ttttattagc agttattttc tctggcttgg tgacttggat tctccgtatg attcccttca
     1021 tcttagtcaa gtataagggc ttgcctgcaa tcgttgagcg ttttttgaag ttcttgcccg
     1081 tttccattat ctttgccttg attctttcaa gcgtagtgac aggtaaggtt gggagccttc
     1141 ctcaaattaa atggctagac ttcttagccg tctttccaac agcttgggta gcctttcgct
     1201 accgcaatct agtcggaaca gttctctttg gagtggtctt gattgccatc ttgcgtttgg
     1261 tcttttaaat tacccataaa aaaaacttat cacagagata gatatcatat aatggcgtaa
     1321 atgctctttt tctgttaaga ttataaggta ttctattttg gaggaaatga catgaaaaaa
     1381 atcgttaaat actcatctct tgcagccctt gctcttgttg ctgcaggtgt gcttgcggct
     1441 tgctcaggtg gtgctaagaa agaaggagaa gcagctagca agaaagaaat catcgttgca
     1501 accaatggat caccaaagcc atttatctat gaagaaaatg gcgaattgac tggttatgag
     1561 attgaagtcg ttcgcgctat ctttaaagat tctgacaagt atgatgtcaa gtttgaaaag
     1621 acagaatggt caggtgtctt tgctggtctt gacgctgatc gttacaatat ggctgtcaac
     1681 aatcttagct acactaaaga acgtgcggag aaatacctct atgccgcacc aattgcccaa
     1741 aatcctaatg tccttgtcgt gaagaaagat gactctagta tcaagtctct cgatgatatc
     1801 ggtggaaaat cgacggaagt cgttcaagcc actacatcag ctaagcagtt agaagcatac
     1861 aatgctgaac acacggacaa cccaactatc cttaactata ctaaggcaga cttccaacaa
     1921 atcatggtac gtttgagcga tggacaattt gactataaga tttttgataa aatcggtgtt
     1981 gaaacagtga tcaagaacca aggtttggac aacttgaaag ttatcgaact tccaagcgac
     2041 caacaaccgt acgtttaccc acttcttgct cagggtcaag atgagttgaa atcgtttgta
     2101 gacaaacgca tcaaagaact ttataaagat ggaactcttg aaaaattgtc taaacaattc
     2161 ttcggagaca cttatctacc ggcagaagct gatattaaat aatttcttga aatcatccat
     2221 tctgagtaag gtgggtggtt tctcagtttt tagggatata gttttaaact atatatctga
     2281 ctatctaata acaatttgtg aaatcaaaca aattgtgaga tactagtacg gtattatttt
     2341 taaggagaaa gaatcatgaa aatcaaaaaa tggcttggtc tagcagccct tgctacagtc
     2401 gcaggtttgg ctcttgcagc ttgcggaaac tcagaaaaga aagcagacaa tgcaacaact
     2461 atcaaaatcg caactgttaa ccgtagcggt tctgaagaaa aacgttggga caaaatccaa
     2521 gaattggtta aaaaagacgg aattaccttg gaatttacag agttcacaga ctactcacaa
     2581 ccaaacaaag caactgctga tggcgaagta gatttgaacg ctttccaaca ctataacttc
     2641 ttgaacaact ggaacaaaga aaacggaaaa gaccttgtag cgattggaga tacttacatc
     2701 tctccaatcc gcctttactc aggtttgaat ggaagtgcca acaagtacac taaagtagaa
     2761 gacatcccag caaacggaga aatcgctgta ccgaatgacg ctacaaacga aagccgtgcg
     2821 ctttacttgc ttcaatcagc tggcttgatt aaattggatg tttctggaac tgctcttgca
     2881 acagttgcca acatcaaaga aaatccaaag aacttgaaaa tcactgaatt ggacgctagc
     2941 caaacagctc gttcattgtc atcagttgac gctgccgttg taaacaatac cttcgttaca
     3001 gaagcaaaat tggactacaa gaaagcactt ttcaaagaac aagctgatga aaactcaaaa
     3061 caatggtaca acatcattgt tgcgaaaaaa gattgggaaa catcacctaa ggctgatgct
     3121 atcaagaaag taatcgcagc ttaccacaca gatgacgtga aaaaagttat cgaagaaaca
     3181 tcagacggtt tggatcaacc agtttggtaa taagaaacag ggaggtggga gagagaattc
     3241 cacctcttgc ttttgtatag agcacggagt gtaaagaaga ttattcgttc atagaaaggt
     3301 agagagaata tggtttttcc tagcgaacaa gaacagattg aaaaatttga aaaggatcat
     3361 gtagtccaac attattttga ggttttgcgt accttgattt ctaagaaatc agtctttgcc
     3421 cagcaggttg gactcaagga agtcgcaaat tatctgggtg agattttcaa gcgtgttgga
     3481 gctgaagtgg agattgatga gagctataca gcgccctttg tcatggcaca tttcaagagt
     3541 tcgcgtccag atgccaagac cttgattttc tataaccact atgacactgt gccagcggat
     3601 ggggatcagg tctggacaga ggctcctttt acgctttcgg tccgcaatgg cttcatgtat
     3661 gggcgtgggg ttgatgacga caagggtcat atcacagctc gcttgagtgc tttgagaaaa
     3721 tatatgcagc accatgatga tttacctgtc aatatcagct ttatcatgga gggagcggag
     3781 gaatcggctt caacagacct agataagtat ttggaaaagc atgcagacaa actccgtggg
     3841 gcggatttgt tggtctggga acaagggacc aaaaatgcct tggaacagct ggaaatttct
     3901 ggtggcaata aggggattgt gacctttgat gtcaaggtaa aaagcgctga tgtggatatc
     3961 cactcgagtt atggtggtgt tgtggaatca gctccttggt atctcctcca agccttacag
     4021 tctcttcgtg ctgcggatgg ccgtatcttg gttgaaggct tgtacgaaga agtacaagag
     4081 cccaatgaac gagaaatggc cttgctagaa acttatggtc aacgaaaccc agaggaagtt
     4141 agtcggattt atggattgga gttgcctctc ttacaggagg agcggatggc ctttctaaaa
     4201 cgtttctttt tcgagccagc gcttaatatc gaaggaatcc agtctggtta tcaaggtcag
     4261 ggtgttaaga ctattttgcc tgcagaagcc agtgccaagc tagaggttcg tctggttccg
     4321 ggtctagaac cgcatgatgt tctggaaaaa attcggaaac agctagacaa aaatggcttt
     4381 gataaggtag aattatacta taccttggga gagatgagct atcgaagcga tatgagcgca
     4441 ccagccattc tcaatgtgat cgagttggcc aagaaattct atccacaggg cgtttcagtc
     4501 ttgccgacga cagcggggac aggacctatg catacggtct ttgatgccct agaggtgcca
     4561 atggttgcat tcggtctagg aaatgccaat agccgagacc acggtggaga tgaaaatgtg
     4621 cgaatcgctg attattatac ccatatcgaa ttagtagagg agctgattag aagctatgag
     4681 tagagatatt atcaagttag atcagatcga tgtgactttt caccaaaaga agagaaccat
     4741 cacagcggtt aaggatgtga ccattcacat ccaagaaggg gatatctacg gaatcgttgg
     4801 atattctgga gcagggaaat caacccttgt acgggtgatt aacctcttgc aaaaaccatc
     4861 tgcagggaaa attaccattg acgacgatgt gatttttgat ggtaaggtga ccttgacggc
     4921 agagcagttg cgtcgtaaac gtcaagatat cgggatgatt ttccagcatt ttaacctgat
     4981 gagccaaaag acagcagagg agaatgtagc ctttgccctt aaacactctg gactcagcaa
     5041 ggaagaaaag aaggctaaag tagctaagtt gttggacttg gttggtttgg cagatcgtgc
     5101 tgaaaactac ccttcacaac tatctggagg gcaaaaacag cgtgtggcaa ttgcgcgtgc
     5161 cttggccaat gatccaaaaa tcttgatttc agacgagtca acttctgccc ttgaccctaa
     5221 gacaaccaag cagattttgg ccttgttgca agatttgaac caaaaattag gattgacagt
     5281 tgtcttgatt acgcatgaaa tgcagattgt caaagacatt gccaaccgtg tggcggttat
     5341 gcaggatggg catttgattg aagagggaag tgtccttgaa atcttctcaa accctaaaca
     5401 acctttgact caagacttta tctcaacagc cacaggtatt gacgaagcca tggtcaaaat
     5461 cgagaagcaa gaaatcgtgg aacacttgtc tgaaaacagt ctcttggtgc aactcaagta
     5521 cgctggagct tcaacagacg agccactttt gaatgaattg tacaagcatt accaagtaat
     5581 ggctaatatt ctctatggga atatcgaaat tctcgatggt actcctgttg gagaattggt
     5641 ggtggtcttg tcaggtgaaa aagcagcgtt ggcaggtgcc caagaagcca ttcgtcaagc
     5701 aggtgtacaa ctaaaagtat tgaagggagg acagtaagat ggaatcattg attcaaacct
     5761 atttaccaaa tgtctataaa atgggttggg ctggtcaggc aggctgggga acggctatct
     5821 acttaactct ttatatgaca gttctttcct tcattatcgg aggcttcttg gggctagtgg
     5881 caggtctctt tctcgtcttg acagcgccag gtggtgtctt ggagaataaa gtcgtattct
     5941 ggattttaga caaaattacc tcaatttttc gtgcggttcc ctttatcatc ctcttggcaa
     6001 tcttgtcacc actttctcac ttgattgtta aaacaagtat cgggccaaat gcagcccttg
     6061 tcccactttc ttttgcagtc tttgccttct ttgcccgtca ggtgcaggtt gtcttggctg
     6121 aattggatgg cggtgtcatt gaggcggctc aagcgagcgg agcgactttc tgggacatcg
     6181 tgggtgttta cctatcagaa ggtcttccag atttgatccg tgtgacgact gtgaccttga
     6241 tttcccttgt tggggaaaca gctatggccg gtgcggttgg agctggtggt atcggtaacg
     6301 tagccatcgc ttatggattt aaccgctaca atcacgatgt gaccatcttg gcaaccatta
     6361 ttatcatttt gattatcttt gcaatccaat tcttgggaga tttcttgact aagaaattga
     6421 gccataaata aaaaagagcc gcgtggctct ttttaactga tcagattttc tgtgcaaatt
     6481 ttttactcaa ggcttgtcca atcaaggcac ccactagggc tccgatgaca atacttgcga
     6541 taaatagaag gacagttcta gggtttggag cgaccatgat acggtcgata tattcttggg
     6601 attttcctct tgccagaaga gtagccatat aggctttggg cgcaatccac ataagcaaga
     6661 ttggtcctgt tgtactaaag gcgaaaataa tgaaagaaag gaagttcttt gttttgtcct
     6721 tgtattttcc taaatgagct actccatctg ctaggaggcc acagataatt ccaggaagga
     6781 aggaaccagc accgtgttta gttcccaaga aaaagagggc aatgacaagg ccgatagtgg
     6841 taatggctcc aaagcgcgga acttttgcga ctaggatcat gtagacgcta ccgccgacaa
     6901 gggcagtaaa ggcaggcgca taaaacatgt ttccagtttg gtcaaagaga ttgcccaaaa
     6961 ggacaccaat ccccatgcag aggaagtaaa gaactgcaaa aagcagtgta gttaagatag
     7021 atttcttcat gaattgtctc ctgataattt tttcacaatt ctcattttac catggtttgg
     7081 tgggattgta aatggatact agaagttttt gaaaacgctt gaaatatgat ataatggttt
     7141 catagttatt tttaggggaa tatcatgggt agatttttag actttgtctt taatcgtttc
     7201 tttttaggga tgattgcgac agccttcttt tggctattaa ctttagcagg agggattatc
     7261 cttggtctag cgccggctag tgccaccttg atgagcttat atgcagaaca tggttatagc
     7321 tttcgggaat acagtttgaa ggaggcttgg tctctttaca agcaaaattt tatctcaagc
     7381 aacctgattt tctatagctt tttaggtgtg ggtctagttt tgacctatgg tttgtatctc
     7441 ttggtgcaat tacctcatca gaccattgtt catttgattg cgaccctttt gaatgtccta
     7501 gtagttgccc tgatcttttt ggcttataca gtatctttaa aattacaagt ttattttgcc
     7561 ttgtcctatc gaaatagtct caaattatcc ttgattggca tctttatgag tctagcagct
     7621 gtggctaagg ttctccttgg gactgtgcta cttgtagcaa ttggttatta tatgcctgcc
     7681 ctgctatttt ttgtaggaat tgggatgtgg catttcttta tcagtgatat gttggaacct
     7741 gtctatgaaa tcatccatga aaaattggcg acaaaataga atgaagcagt tttggctaca
     7801 tacgcttcta agaacctata gttcagtgat gatcattatc attgcgagtt ttgcaatctt
     7861 actctcttac gctgactggg attcacgtga aaaggaagcc cagagagtag cccagcgtgt
     7921 aactgctaga acagtgagtg aaattgaata ttaccataga gagtcaaccc agatagctca
     7981 ggctttagtt gaaaatcaag cgcgtatcga gggaatctat aaatacttta gccttagcat
     8041 gccagactat ttttactggc aattagagcg gaaagcttcg ccttatatat cagtctctct
     8101 gtatgaaaat gttgatgacc tctatgttcg aaatgatttt gtaactgggg tggccattgc
     8161 ttttcaagat tacaaggaag tctatgtttc tactaaagac aaacgtagtg gagaaaaaat
     8221 cagggctgag gatttcaaac cagcaggaaa tagttttgcc attccggtgt cagatccagt
     8281 gtcagatcaa gatttaggag tgatttacat ctccttggat cctgctgttt tataccatgc
     8341 cattgataat actagaggtc atactccgat ggcagtaaca gtgacctcac cttttgatac
     8401 ggagattttt catattggtg agacagttga tagggagagt gaaaattggc tagttggctt
     8461 aacttctcat ggatatcagg ttcaggtggc agttcctaaa aactttgttt tacaaggaac
     8521 agtgactagc tctgctttga ttgtgggttt gagccttctc tttattgtca ttctttatct
     8581 gactttgagg cagacttttg ctaattacca aaagcaggta gtggatttag tggaatccat
     8641 tcaagtcatt gctcaaggcg aagaggggcg tcggattgac atttccgaaa aagatcagga
     8701 attactccta atcgcggaga cgaccaatga tatgttggat cgattggaaa agaatatcca
     8761 tgatatttac cagttagagc ttagtcaaaa agatgccaat atgcgagcct tgcaggcgca
     8821 aatcaatcct cattttatgt ataatacgct ggagttcttg cgcatgtatg cagttatgca
     8881 gagtcaagat gagttggcag atatcattta tgaattcagt agtctcttgc gtaacaatat
     8941 ttccgacgaa agagagaccc tcctcaaaca ggaattagaa ttttgccgta aatacagcta
     9001 tctctgcatg gttcgctatc ccaagtccat tgcctatggt ttcaagatag atccagagtt
     9061 agagaatatg aagattccca agtttacctt gcaaccgctg gtagaaaact atttcgcgca
     9121 tggtgttgac cacaggcgga cagataatgt gattagcatc aaggctctta aacaggatgg
     9181 ttttgtggaa attttggtgg tcgataatgg tagaggaatg tcggctgaaa agttggcaaa
     9241 tatccgagaa aaattaagtc agagatattt tgaacaccaa gccagctaca gtgatcaaag
     9301 gcagtctatc gggattgtca atgtacacga gcgttttgtg ctctattttg gagaccgcta
     9361 tgccattacg atagagtctg cagagcaagc cggtgttcag tatcgtatta caattcaaga
     9421 tgagtagaaa gggagaaaat gtataaagta ttattagtag atgatgagta catggtgaca
     9481 gaaggtctga agcgtttgat tccctttgat aagtgggata tggaggtcgt cgcaacagcc
     9541 agtcatgccg atgaagctct agaatatgtt caggaaaatc ctgtcgatgt catcatttcc
     9601 gatgtcaata tgccagacaa aacagggctt gatatgattc gggagatgaa agagatctta
     9661 ccagatgctg cctatatcct gctctcaggt tatcaggagt ttgattatgt aaaaagagca
     9721 atgaacctta gtgtggtgga ctatttggtc aagcctgttg ataaggtaga gctgggaaat
     9781 ctgctggaga agattgcagg tcagctcggc gagagaggga agaaaagtca gactcttagt
     9841 caagaattag acgaggctgg atttgttagt tatttagggg ataaggagaa ttggtggata
     9901 ggtctatcca aggaaaaaca aggttccttc gccattccct actatgtctt gggccaagac
     9961 tggcagattt tcatttctga ccaaccccta gatggtttag tcgttacacc ttttgaagct
    10021 ccttatcaag aacactttga acgctggaag ctgaatgctg agaaaaccct cttttacggt
    10081 tctgtaaatc tgcagcagtc tgagagtctc tttgcctatt acgaaccgat ttatagggtt
    10141 atcattcagg gaaatctcaa tcaaatcgta gaagagttaa atctcttgga gaaggtagtt
    10201 cttgaaaata cgccgcgtgt tccgattact aaacagcttt ttattcagtt tgtcatggat
    10261 gttttccatt tatttgaaca tctcaaagct gatgacctga cagacatcgt caaaaccatt
    10321 catgctattc aatccttcga tgaattggtt ccttatatca aggaaactct gagcagcttt
    10381 ttcggtcaat accgtatgaa tgaaaatgtg gtcagtgtgc tggaagtcat tggtcgtgat
    10441 taccaaaaag agctttccct caaggatatc agtaaggctc tctttatcaa tcctgtctat
    10501 ctagggcagt tgattaagcg tgaaaccgat tcgacttttg cagagttact aaacaaacaa
    10561 cgtattaagg ctgcccaaca acttttgctt tcaactagtg acagcatcga agatatttgt
    10621 tatgctgttg gttacagtaa ccttggatat ttctataaag tttttcgaaa attgtgcgga
    10681 aaatcgccaa aagcctaccg aaaacaggta gaaactatac tataagattt gtattccttt
    10741 acaaaaggtg ctataatatt atcaggaggt tctatcatga aaaagaaacc aatttatcta
    10801 tgggtcttgc taatcttgtc tgcccttatt tcagttccat ctctgtttgg aattgtgagt
    10861 cccttgccta gtaaagaagc ccttcgtgct gctcagaaac aagttgcagg ggtcaatgct
    10921 cagcaattag aagaccagct taattatacc tatagagtag cagaagcatc tcattctatt
    10981 tttaatgttg ctttgattgt gctatctact attttagtgg tcgtagcgat tgtcttcctt
    11041 gttcgtaaaa atttgcaata tgcaaactat acttacgttg gttatgtttt gttagctatt
    11101 attggttcga tttatggcta tgttggttta caagacgctg tgcagttggt tcaagatgag
    11161 agcatgcgtt tgacagtgag tattggttca aaagctgtta gcattttcta tatcgtcatc
    11221 aatgttctct tcctagccct tgtcttctat aagatgtggc gacaacagaa agccttggca
    11281 gaggaagagg aaacagaaga gcttgcctaa gtattgacaa aaaagaacta tcaagataca
    11341 atcttggtag ttgttttttg attatagata aaatcatgga ggtacatatg aagacaattt
    11401 ctttagttta tattagtttg agtggcaaca ctgaaagttt tgtgacgcgc ttgaaagact
    11461 atctcttgtc ccagtacaaa aggattgagg ttcaaaagat tcatatcaag gatttggtca
    11521 aggaaggcaa aaatttctat gaaatggacc atccctatgt cgcttttttg ccgacctatc
    11581 tcgaaggtgg gaatggcgtg gataacggag atgttgagat tttgacgaca ccggtgggag
    11641 attttattgc ctatggtaac aatgctagta agtgttttgg tgtggttggg tcaggaaatc
    11701 gtaactttaa taaccaatac tgcctaacag ccaagcaata cagtcaacgc tttggcttcc
    11761 ctgtattggc tgactttgaa atgcgaggca tgctggaaga tatcaaacat gtcgcagcta
    11821 ttatcgcaga tttgtatgag ttggaaaagg agaattaata ctcaatgaaa atcaaagagc
    11881 aaactaggaa gctagtcgca ggttgctcaa agcacagctt tgaggttgca gatagagctg
    11941 acgtggtttg aagagattct cgaatagtat aaagttctta taagaggtgg ttgaagttgt
    12001 tcaactgttt tttgagtata aacagtcttt aagaacgtaa aaaagcataa gttcaggtct
    12061 ttaaaacctt gttcttatgc tttttatcat agaaatattt gctgaatttt ctcttgaaat
    12121 gaaaccttat cttcatctcg cgcttgtccc ttaccctgct tttccgattg cgagtgcgta
    12181 gataaagaag atggcgaagc caaagaggaa aatcaatccc gcaatggcaa ggtgtttgag
    12241 ccaagaagga agatttttgt tttcacgcgt taccaaggcg taattcaaaa gagcaaagaa
    12301 aggtgttgtc aggaaagatc caatcatggc aaagcggagc atggttgaaa cctgaccagc
    12361 gaagaacttg ataatgacga taccgatgat agcagtgatg gtcatccaga tgttcaaaga
    12421 tttacgattg tcctcttttt gactgattag cagtcggaga gattcctgat taacgcgaga
    12481 atagccatcg ataacagtta taactgttcc aaagatacag aggaaggcaa taaaggtaat
    12541 caagtaacgg gaccattcgc caagaacaga ggcatacatg cccacgaatt gagagatgta
    12601 tttggctgaa gcagcttcaa ccgcctgccc tgtaggatac tgaatcagtg ctcccagtgc
    12661 cacaaagaag acggctagga tagctgttcc aatataacca gtgttaaagt caaacagagc
    12721 gtcttctgtg ttaaagttga cggtctttct cttttcagct gaccaaagtg aattgatggc
    12781 tgaaatttca ataggagccg gcatccatcc taggagggag acgatgaagg gcagagctgc
    12841 catttgccaa ggtgtcttct cgacaaaatc agaactgtat tctggatgct tgaccgctgc
    12901 tatgataact gccagaacag tcgcaatggt taaggcagac ataatccatt tgaccatgcc
    12961 gtctaaaagt ttgtagcctc caaagagtag catagcccaa atgattgcaa cgagaatgag
    13021 ggaccactga gtaatgctaa gtccaatcat tgggaaggca ctggcgatga tagctgagca
    13081 cagaatggca acaccagccg tgttgaccat agccgaaaag acattgagaa taaagaaaat
    13141 ccagagatag agttttcctt tttcggcata accttcaacc aaagtctttc cagtatcagc
    13201 tgtgtattca gcaccaaaac ggaaaaatgg atatttaaag acattggcta agatgaccaa
    13261 gagaagtaga gaccaaccgt aagaaccgcc agcttgagtt gaggatacaa tgtgggaacc
    13321 tccaacagcg gcagttgcca ttaggattcc aggtcccatt gcttttagct tgcttgccca
    13381 ggttgattga tttaaggaaa tagcttgcga catgataaat actccttttt gaattttcag
    13441 aataatctgt atatgcaatc tatattctac aaaaaatttt ggaaaaatgc aagaatattt
    13501 tgaaaaaaga tagaaaattt acaaagaaaa tctgaaataa ctgttatgat atagcagatt
    13561 gaaactagaa tagtacactt ttgcttctaa aacattgtta gaaatcgatt tgactgtcct
    13621 gattgattta tcctgttctt atttcatttc actatagtaa ggtgcagcag gataaagcaa
    13681 aaccgagaag tttctttctc ggttttgctt attatgaggt tcctattttc tatgctccaa
    13741 gagttgattg atgtgatcta cttttttagc ctctctttta tagagaatag cccagatgat
    13801 gagatagaca atcgcaaact cgataatgag ctggaggtag aaaatccagt ggaaggggaa
    13861 ccaaccagca agagttgcta gtgggacaaa gccagccagc ataaggaaga aatgagtcag
    13921 agtggcacga agcatgctcc agtcacggct gaataagcgg ttgccaaagt tgaagagcat
    13981 accgatggct gcccagataa gtgtgcagta gagcaagacc agggcaccgt gaacctgatg
    14041 ctgatccatc acttggccga tgagagagta gggatttagt ggtgcgtagg tatttggtgc
    14101 ataaatgagt gaaaagagga tggagaggat gaggccgata agaacaccgg cagctgcatc
    14161 gtgaaatact tgttttttca tagttctaat ttctccttga tggtttttag ataacggcgt
    14221 gaagagtagg tgaagctttc gtttttcaag aaaatttcta ccagaccgtt tggcgtgagc
    14281 ttgaggtgag agatggaatc gatattgatg atttctgatt gggaaatttg gataaaattg
    14341 gttggcaaga gtttaagaac ctgatagagt cgcaaatcaa tgctgtaggt ctgactcgcg
    14401 gtttctgcta gaaccttccg attctcgata tagaagcgct gaatcttgcc aatctcaact
    14461 agatagacct gatcatcgat ttttcctttg attttttctc tttggtccag attttctgcg
    14521 aactcgatga ctttctggac tttttcggtt tcttgaggtg cttggacaat cagcttttcc
    14581 tcctcgtaag tctcactaat ctgtagttct actttcataa atttctctcc ttgtttttca
    14641 tacaaaatta atctcacttc ttgtacacct ttattaatac tcttcaaaaa tctcttcaaa
    14701 ccacgtcagc gtcgtcttgc cgtaggatag ttgttgactt tgtcagtttt atctacaacc
    14761 tcaaaacagt gttttgagct gtctgcgtct agctttctag tttgctcttt gatttttatt
    14821 gagtatacca ctattttact ccctctggca agggactttg tctatgtgga gggattgggc
    14881 tcctatgtgg tggagttttt ctgttctttc tgaaatatgg tataatagca ctaattaatt
    14941 tctaggaaaa tagatacaga aaggggctga aagatgtctc atattattga attgccagag
    15001 atgctggcaa accaaatcgc agctggggag gtcattgaac gtcctgccag tgtggttaaa
    15061 gagttggtag aaaatgccat tgacgcgggc tctagtcaga ttatcattga gattgaggaa
    15121 gctggtctca agaaggttca aatcacggat aacggtcatg gaattgccca cgatgaggtg
    15181 gagttggccc tgcgtcgcca tgcgaccagt aagataaaaa atcaagcaga tctctttcgg
    15241 attcggacgc ttggttttcg tggtgaagcc ttgccttcta ttgcgtctgt tagtgtcttg
    15301 actctgttaa cggcggtgga tggtgctagt catggaacca agttagtcgc gcgtgggggt
    15361 gaagttgagg aagtcatccc agcgactagt cctgtgggaa ccaaggtttg tgtggaggat
    15421 ctctttttca acacgcctgc ccgtctcaag tatatgaaga gccagcaagc ggagttgtct
    15481 catatcattg atattgtcaa ccgtctgggc ttggcccatc ctgagatttc ttttagcttg
    15541 attagtgatg gcaaggaaat gacgcggaca gcagggactg gtcaattgcg ccaagcaatc
    15601 gcagggattt acggtttggt cagtgccaag aagatgattg aaattgagaa ctctgaccta
    15661 gatttcgaaa tttcaggttt tgtgtcttta cctgagttga ctcgggctaa ccgcaattat
    15721 atcagcctct tcatcaatgg ccgttatatc aagaacttcc tgctcaatcg tgctattttg
    15781 gatggtttcg gaagcaagct tatggttgga cgttttccac tggctgtcat tcacatccat
    15841 atcgaccctt atctagcgga tgtcaatgtg catccaacta agcaagaggt gcggatttcc
    15901 aaggaaaaag aactgatgac cctggtttca gaagctattg caaatagtct caaggaacaa
    15961 accttgattc cagatgcctt ggaaaatctt gccaaatcga ccgtgcgcaa tcgtgagaag
    16021 gtggagcaaa ctattctccc actcaaagaa aatacgctct actatgagaa aactgagccg
    16081 tcaagaccta gtcaaactga agtagctgat tatcaggtag aattgactga tgaagggcag
    16141 gatttgaccc tgtttgccaa ggaaaccttg gaccgattga ccaagccagc aaaactgcat
    16201 tttgcagaga gaaagcctgc taactacgac cagctagacc atccagagtt agatcttgct
    16261 agcatcgata aggcttatga caaactggag cgagaagaag catccagctt cccagagttg
    16321 gagtttttcg gacaaatgca cgggacttat ctctttgccc aagggcgaga tggactttac
    16381 atcatagatc agcacgctgc tcaggaacgg gtcaagtacg aggagtaccg tgaaagcatt
    16441 ggcaatgttg accagagtca gcagcaactc ctagtgccct atatctttga atttcctgcg
    16501 gatgatgccc tgcgtctcaa ggaaagaatg cctctcttag aggaagtggg cgtctttcta
    16561 gcagagtacg gagaaaatca atttattcta cgtgaacatc ctatttggat ggcagaagaa
    16621 gagattgaat caggcatcta tgagatgtgc gacatgctcc ttttgaccaa ggaagtttct
    16681 atcaagaaat accgagcaga gctggctatc atgatgtctt gcaagcgatc tatcaaggcc
    16741 aatcatcgta ttgatgatca ttcagctaga caactcctct atcagctttc tcaatgtgac
    16801 aatccctata actgtcctca cggacgtcct gttttggtgc attttaccaa gtcggatatg
    16861 gaaaagatgt tccgacgtat tcaggaaaat cacaccagtc tccgtgagtt ggggaaatat
    16921 taaaagtata aaaaagtctg ggaaaaattt tcaaaatcag aaaaacgcat aaaatcaggt
    16981 gttcaaaaac cttgatttta tgcgttttat catggaaata gttacttcat gttttcctaa
    17041 ttcttttcga aactcttttt aaaccacgtc agttttatca gtaatctcaa aacagtgttt
    17101 tgagctaatt ttgccagttt tgtctgtaac atcgaagttg tgttttacca ctctgcgact
    17161 ggtttcctag tttgctctat gattttcaca gagtattaaa ttgcgatttt gccaagtttc
    17221 tttattcgtc taaaagtaga gtctgttcta tgcgtctaat gtacgaatta ggttgaccat
    17281 ttcaatagct ccttgtgcac actcagaacc cttatttcct gctttggtac cagctcgttc
    17341 tatggcttgt tcaattgtat ctgtcgttag cacaccaaac ataacaggaa tttcgctatt
    17401 taaactgatt tgggcgattc ccttagatac ctcgctacat acataatcat aatgacttgt
    17461 attccctcta atgacagctc ccaagcagat aattgcatca tattttttac tttttgccat
    17521 ttttgatgca atcagtggta tttcaaaagc tcctggaacc caggctacct ctatatcttt
    17581 ctcgtttaca ttctctcttt tgagattatc tagtgctcca gataataatt ttgaagttat
    17641 aaattcatta aatctcgcta caacaatacc tattttaata ttgtttgcta ctaaattacc
    17701 ttcataagtg ttcatttatt tttcctccat atttaaaatg tgacccattc gatttttctt
    17761 tgtttctaaa taaaaactat cgtaaggatt ggcttctatt tcgattgata ttctactgga
    17821 aatggtaatt ccatattttt ctaactgttc aaccttgtca ggattatttg tcagtaaatg
    17881 aagtgactga agtcccagat ctttaagcat ttgtgctcca atatgatatt ctcttaaatc
    17941 accttcaaag cctaatgcaa gattggcatc aagcgtatcc atgccttgat cttgtaaatg
    18001 ataggctttt aatttattga taagtccaat tcctcgtccc tcctgtcgca agtaaagtaa
    18061 gacacccgaa ccattctcaa caatcatttt catagcttta tcgaattgct gtccacaatc
    18121 gcaacgtaaa gagcctaaaa catctcctgt taaacattcg gagtggaccc gacataatac
    18181 attggcttca tcctctatgt ttcccataat aagagcaaga tgatgttccc catttagttt
    18241 atctatatag ctaattgctt tgaaattacc gtatctagta ggcatattga cagttgaaac
    18301 tcgttctacc agctgatcat atacttttct atattcttgt aattctttga tggtaattag
    18361 tggaatgttg tgtttcttcg aaaactgaat taaatcatct gttctcatca ttttgccatc
    18421 atgattcatt atttcacaac ataggccaca ctcttttagt ccagctaatt ttaataaatc
    18481 aacagttgct tctgtgtgtc catttctttc tagaacacca cctttttttg caattaaagg
    18541 aaacatgtgt cctggcctgc gaaaatcaga gggtgttata tcttcagcta cacacatacg
    18601 tgcggtcagt cctctttcct cggcagaaat acctgtggtc gtttctttat aatcaattga
    18661 aactgtaaaa gcagtcttat gattatctgt attgttttca accataggtg aaagcattaa
    18721 ttgattagct aaactttcgc tcataggcat acaaattaat cctttggcat aagtagccat
    18781 aaaattaaca ttttctgttg tagctgcttg tgcagaacaa attaagtctc cttcattttc
    18841 tctatccttg tcgtctataa caagaacaag tcgtcccttc tgcaatgctt ctaatgcttc
    18901 ttgtattttt cgatattcca ttgactgatt atcctttctg ctaaaatcca ttttgatata
    18961 atagttcctt agatatttct gattttggag agttatccat cagtttttgc acatatttac
    19021 ctaagatatc attttcaaga tttactgtac tcccgacttg tttactctta agaatggttt
    19081 gttccaaggt atgagggata acagatactg aaaagtttac tttggagact ttagcgacag
    19141 tcagactaat gccgtcaatt gtaatagatc ctttttcaac tattaaatct aaaatttctt
    19201 tttgtgtgtt gatttgatac catacagcat tatcatcttt ttttattgac gagatttttc
    19261 ctgtaccatc aatgtgtcct gtaacgacgt gacccccaag tcgaccgttg acagataagg
    19321 ctctttctag attcacctca cttccatgtt ttaatagagt aagagctgtt cgactccatg
    19381 tttcattcat tacatcaact gtaaaggatt gatgattgaa atgagtaact gtaagacaga
    19441 taccatttac tgctatacta tcgcctaaat ggatatccgt taatattttt gaggctttaa
    19501 ttgatagttt acaattacga gagtctttct gtattctttc aacttttccg atttcttcaa
    19561 ttattcctgt gaacatggat aaatcacttc actttctatg agatagtcat ttcctatttg
    19621 agaaaatgca taaggtttca atctaatagc gtcatttggc aaagaaatgc cttcacctcc
    19681 gacaggaaac ttggcactgc ctccaaaaat ttttggtgca atatatattt tcagctcatc
    19741 aacaatttgt tgttccaaag cactccaatt cattagactg cccccttcta gaactaggct
    19801 atcaatctgc atgtttccta gatgttgcat taaactcgat aagtctatat gattgccttt
    19861 tttctttatg gaaagtattt cacagccatg attttgatat agcttcattt tatttttgtc
    19921 ttcagaggaa gtggcaatgt aagttttaat atcatttgct gtttttacga ttttagaggt
    19981 aagaggagtt cgtaaatgtg tatcgcatat gatacggata ggatttttcc cttcctccaa
    20041 tctacatgtc agcaaaggat cgtcttgaat aacagtattg actcccacca taattgcact
    20101 aacatagtgt cgtaactgat gcacatgctt tcttgcttct tcttcagtaa tccatttgga
    20161 ttgatttgtt ttagtggcta tttttccatc cattgacatt gcatatttca taaaaacata
    20221 gggtacatgc tgggtaatat actttctaaa actttttatt aagttaagac actcattttc
    20281 taaaattcca acagtaactt gaagattatt ttcctcaagt atctttactc cttttccaga
    20341 tacaatagga ttacagtcta ggcttccaat gactactctt gtaataccac tatcgattat
    20401 agcatctata cagggaggtg ttttcccgaa gtgacaacag ggttcaagtg ttacataaag
    20461 cgtcgctccg acaggggatt ctctacagtt tttaagagca tttctctcag catgtgggcc
    20521 accaaaaaac tcatgataac cttgtccgat gatgtgatta tcttttacaa taactgcgcc
    20581 gaccatagga ttgggattga cgtaaccagc ccctttttgt gccagtttta ttgctaattt
    20641 catatatttt gaatcgctca tctcactacc tccaaaaaaa atataccttg aataggggac
    20701 tactcaaggc atacaaaaga aaacttatgc gattaacaaa aatactctga aatgacaagt
    20761 aatcatttca gagcacgcaa aaagcacaaa tatactttta tcttctttca tccagactat
    20821 actgtcggct ttggaatttc accaaatcat gcctttcggc tcgtgggcta taccaccggt
    20881 agggaattac accctgccct gaagatagtt attcaattac agatgattat agtacttaat
    20941 tttgaatatg tcaacagata aataccgatt gtttttgata tactgtattt gtgataatcg
    21001 attctcgctc ctcggataaa gaaaatatga tatactagat aaacgaaata agagagaagg
    21061 aatactatgt acgcatattt aaaaggaatc attaccaaaa ttactgccaa atacattgtt
    21121 cttgaaacca atggtattgg ttatatcctg catgtggcca atccttatgc ctattcaggt
    21181 caggttaatc aggaggctca gatttatgtg catcaggttg tgcgtgagga cgcccatttg
    21241 ctttatggat ttcgctcaga ggatgagaaa aagctctttc ttagtctgat ttcggtctct
    21301 gggattggtc ctgtatcagc tcttgctatt atcgctgctg atgacaatgc tggcttggtt
    21361 caagccattg aaaccaagaa catcacctac ttgaccaagt tccctaaaat tggcaagaaa
    21421 acagcccagc agatggtgct ggacttggaa ggcaaggtag tagttgcagg agatggcctt
    21481 cctgccaagg tcgcagtgca agcaagtgct gaaaaccaag aattggaaga agctatggaa
    21541 gccatgttgg ctctgggcta caaggcaaca gagctcaaga aaatcaagaa attctttgaa
    21601 ggaacgacag atacagctga gaactatatc aagtcggccc ttaaaatgtt ggtcaaatag
    21661 gagcagagaa tgacaaaacg ttgttcgtgg gtcaagatga ccaacccgct ctacatcgcc
    21721 tatcatgatg aggagtgggg tcagcccctc catgatgacc aagcattgtt tgagttgttg
    21781 tgtatggaaa cctatcaggc aggcctgtct tgggaaacgg tactcaacaa acgccaagct
    21841 ttccgagaag cctttcatag ctatcaaatt cactcagtcg cagagatgac tgagactgaa
    21901 ttggaagcca tgctggagaa tccagctatc attcgaaata gagccaagct ttttgctaca
    21961 cgcgctaacg cccaagcctt tctacagtta caggcagagt acggctcttt tgatgcctat
    22021 ctttggtctt ttgttgaggg gaaaactgtc gttaacgatg ttcctgatta tcgccaagcc
    22081 ccagctaaaa cacccttatc tgagaaatta gccaaagatc tcaaaaaacg aggcttcaag
    22141 ttcacaggcc cagtcgccgt attgtctttt ctacaggctg cagggctagt tgatgaccac
    22201 gagaatgatt gtgagtggaa aggtcttaaa tgatgtctaa caaaaataag gaaattctga
    22261 tttttgcgat tctctataca gtcctcttta tgtttgatgg cgttaaattg ctggcttctt
    22321 taatgccatc tgccattgca aattatcttg tttatgtagt tttagcttta tatggctcct
    22381 tcttgttcaa ggatagattg atccaacaat ggaaggagat tagaaagact aaaagaaaat
    22441 tcttctttgg cgtcttaaca ggatggctct ttctcattct gatgactgtt gtctttgaat
    22501 ttgtatcaga gatgttgaag cagtttgtga gactagatgg acaaggtcta aatcagtcta
    22561 atattcaaag tacctttcaa gaacaaccac tactgatagc tgtttttgct tgtgtcattg
    22621 gacctctggt agaagaatta tttttccgtc aggtcttatt gcattacttg caggaacggt
    22681 tgccaggttt actaagcatt attctggtag gacttgtttt tgctttgact catatgcaca
    22741 gtttggctct atcagagtgg attggtgcag ttggttactt aggtggaggc cttgcctttt
    22801 ctattattta tgtgaaagaa aaagagaata tctactatcc cctacttgtt cacatgttaa
    22861 gcaacagcct ctccttaatc attttagcta tcagtatagt aaaatgaaat aagaacagga
    22921 caaatcaatc aggacagtca aatcgatttc taacaatgtt ttagaagtag aggtgtacta
    22981 ttctagtttc aatacactgt aacatgtgat gaaaatgcca gtaatgatac cgagaaaaaa
    23041 gctgagaaac ttttcccagc tttatttgtt atagtcaaag cgaatgactt gttcctgtgc
    23101 atctacatga gcatggaccc caaagggtac aattgctctt ggagttgcgt ggccgacatt
    23161 cagattatag acaatcggga tattgctgtc aatgatatct aatagtgcct ctttatagtc
    23221 gtcatggaaa gtttcatcca taggttttcc gaccaagagt ccattgatga ccgcgaatat
    23281 gccagtgtcc tttaaagtta gcaacatctt tttgaagtct tctggcttag gcttttcttc
    23341 gcttgtttcg agcaagagga ttttcccttc ccagtctgac aagtcaggga aaagtttgta
    23401 ttttgggcag agttccgtgc tatctgcgta tcgagagttg tcaaagatat cgtagaggga
    23461 ttctaggcaa ccaccgagga ttttcccctc gaactgagca cttccttgca acaagtcaaa
    23521 acctgtattt gtatgactga cacgaggtgt tcccagggcc gtgggactaa aatcagttcg
    23581 ttcctcatac caaacgtcac tagggcggat ttctgaaatt cttcccgtct caatcaattc
    23641 tttaaagtag tgaaggctat aggctagcat ttctttgtct aattcacaaa tgtctgctaa
    23701 aaaggattga ccataaaaag tcttgattcc taatttatgc aacatgaggt ggttcatggt
    23761 tgtatccgag aagccaagaa aaattttttg cttgataacc ttttggagtt ggtcattttc
    23821 aaaaagataa ggtagcaagc gataggtatc gtctccaccg atggcacata ggatcatgtc
    23881 gatgctatca tcagaaaagg catgaatcaa atcctctgca cgagcttcag gatggtcctt
    23941 gataaagtct aatcctttta acgaatgggg caaaaagatg ggattgagtc ccagatcctt
    24001 gagacgttgg atacccaagt ccacttcgtg tttgacaaaa tcctctccga taatgccact
    24061 agataaacta acaataccaa tagtagaaac catatctcat cctcctagaa atagattgag
    24121 cctattttat cacaaaatat aagagaaagc tatgtgggat ttcagaagaa tacttttaat
    24181 actcttcgaa aatctcttca aaccacgtca gttttatctg caacctcaaa gctgtgcttt
    24241 gagcaacctg cagctagctt cctagtttgc tctttgattt tcattgaata taactcaaaa
    24301 aagtaagtga aaaagcaacc gcacctgcag ttgcttttat cgttttctta ttcttgatag
    24361 cgtgttggtc ctgcacttgc actacggata ttcaagcgtt tcttgagata gaagcgaagg
    24421 gctaggaggg ctgctccaat aatagctagt ggcaatggat ccagtactgg gttaaggcta
    24481 gctggtagga agcttgttgc aaagaagaca agcaaccaaa ggaacataga agctaggata
    24541 actagtacag atttccagaa aggtggacgt tgactgcgat ccatatctgg tccatagtat
    24601 tggtaaacaa agtagtacat caagtagaag gcaaatccac caaccagtcc aactaataga
    24661 agagtaatca atccatagcc gaaagcttgg tctgccgcaa agaaggttgt gagggcgctg
    24721 acaagggcaa agaggctagt gatgaaaaga gctgagtcca taatcatcag ttttgggtca
    24781 tcattttctt ttggatgctc tttttcgtac tgctctttga cagtgaagct atgagcccaa
    24841 tgagttggtg cgccgtatag ggaacgggca gttgtacctt tagattgctc ctcaaggatt
    24901 tggggaatga cttcctcaaa aatagtctgg atttcagcgt ctgttttccc atctttgatg
    24961 aattgttggg tagcaatgtg gacaaactct tggtttttct tagttaattt ttgtagatca
    25021 atttgagaca taggaactcc tcttagaacc attttttatg gatgagatag agagtgagcg
    25081 agacactcat agcaaaggcg ataaagacga ttaaccagaa ggcatttggc tctccgttta
    25141 gggggatttc attatcctta aagttcatcc cgtaggcaga aaagaccatg gttgggatgg
    25201 acatgacgat ggtcacaagg gccaaggttt tcatgatgtt gttctggttg ttagaaatga
    25261 tagaggcaaa ggtctctgtc atagaatgca agacgtttcc ataaatatct gccatctcga
    25321 tggcctgttg ggtttcaatc agggtgtctt caagcaggtc ttcgtcctca aggtatttct
    25381 tgatattgct ggttgaactg gtcaatttct taatcacgcg ctcatttgtt ttgagggagg
    25441 ccttgaaata gacgatagtt ttttccaatt ccatgagctc aatcaattct tcattacgag
    25501 ttgattgatg cagttgactt tcgatttgtt cactcttgcg gtcgattgaa cgaagggctg
    25561 ttaggtaaag ctctgcattg cgataaagaa tttgaaagat aaaacgtgaa cgcatgaagg
    25621 tatagaaatt acgcaatcga cggttgataa agacatcaag gacaggtagt ggttccaaac
    25681 acgtagtgat aatggtttcc tcagtgatga taataccaag cgggatggtt acgtagtagg
    25741 tgcggttatt tctttcctcc gtgaccggca cgtctacgat aatcagggta tactcgtctt
    25801 caatggtaat acgagacatt tcttccgcat cgagcggtgc tcgaaggtcg gcaatatcaa
    25861 tatcgaaggt gttggcgatt tcgagtgatt cattttgagt cggattgacg agattgatcc
    25921 aagtacccgg ttcaagcgta tcgatctctt taaattcagt tgttgtagag agaaaaactt
    25981 gtttcatatc ccttatcctt tctcattttt cagatttttt cacactgtac tattatacta
    26041 caaaatcggc ctttttcaaa ataaatctaa caaaaaacct gttgctattg gatttttgct
    26101 ttgtcttgaa atgttgattt ttaaatttgc tccccttttt gctcccccta tagtgctttt
    26161 agtgcagttt cgtagaatga tactgctttt tttgcgttct cttttgagag gtggctgtat
    26221 atatccatgg tcattgataa agtggaatga cctaggcggt actggagttc cttgtatggt
    26281 attccagagt taagcaagag actagcgtgg gtgtgacgga atccatgaaa gccaatgtta
    26341 ggaacatcag cacgcttaaa atgtgtttgc aatcttcttt taagtcttga actgctagga
    26401 tactcatgaa tgaaatcaga aaatactaca ctttcacgtt gccctatctt ccaagcctct
    26461 ttagtttggc gtagtttgta ctgcttcagc atgctaacag tcgcttgatc tatatctata
    26521 tcccgataac tagccttaga ttttgggcta ttaatttcta aatcacgatt tagtgttttg
    26581 gtaacatgta ccacggcatt atctaaatct atatcagacc aggaaagagc taaagcttca
    26641 ttgatacggc aaccagtagc caataggaac ttatagagcg ttgtctcata gtagtaacga
    26701 tatctatcgc tatctaaatt atcgagataa gcaagaaact tttttagttc ttgattttca
    26761 aagtgtttta cttttttccg ctttgctttc tgagtgttac gaggtaaaac aacatcacgg
    26821 gcaggattgg acggtatagc ctgcatagtt acgccatact gtaagatacg tttgtttaga
    26881 gcgtgtatct tgtcataatg tagaaaagcc cctttttccc ctctattagt cttgtcagca
    26941 agttcgttga taattgactg gataagtgga gtagtgagtt tatcgagctt ataagcccca
    27001 aaaataggca atatatgatt atcaattaac tttctaacat tgtcttgagt gtttggctta
    27061 accgtattct tataactttc taaccagaga ttggctaatt cttgatagtt ttttatggtg
    27121 ctagcctttt gtctagtaga tcctgctttt tgaaaagcta ttttttcttg agtagctttc
    27181 tgcttaactt ccttttgtgt ccgtcccgtt accttagttt taactttctt tcctgtaacc
    27241 tggtcaactc ctagatatat attagcacga tagactttac taccgtcttt ttttataact
    27301 tcagttattt tcatgattta aacctttcta aaatacatca gcaggcaagc cattatcaaa
    27361 aaggttttat atcatgctag ggcttcacag attgccctgt attcaagttt aacaagttag
    27421 ataggtaaat tatatgctta attgttaagt ggtaaaaacc cttattttac agtgtttttt
    27481 gagagttttt ttctaaagtt agtttaccag ttagctaaca aaacacttta catttcccac
    27541 gcgcgtggta taatagaatt aacaagataa cttgcgaagg attaacgttg tgttcccaaa
    27601 ggggagtaag tcaatggacg agaattcaca tatgcctggg gttatcttat tttttttgtc
    27661 tttttttcaa gttttctacg aacgtttcag gatttcgttc gatttcctca attataaaat
    27721 cgcagaggat ttgagagtag ccgtatcgag taccaatttt ataatggtag caataacgtt
    27781 gatttgtctt gatatcataa aatgtattaa aaagttgtaa gtcgtttgtt gtaaacgtct
    27841 ttttttgttt ttctccaccg actatttttg ttaatagaat ccctttcgat ttgagatatc
    27901 tattaacaat tttaataact tccctagtag ttaatggata gatagcgttt ggatctttaa
    27961 tttctctaac tatacctata ctcgtttccg cagtgttatc aagtttaaca aataaatcag
    28021 catcttgttt tcgcttagtg atataaaatc gagtttcaac aggtacagca tagtcagaat
    28081 tattagtaga tatttcactt tcaattttat cttgctcggc gagtatacgt tcagccattt
    28141 taggagagta cttggctcga atttcttctt gattaagttg gtcgatgcgg actgataaag
    28201 tcaagaaatt ttgtgcaatt tgttttgtga tatcctcatt gtgaaactct tgcattttta
    28261 aaatataatt agaaacacat gcttgaaata atggggcata gatatgttca taatcttctg
    28321 ttatgaagtg ggtactagta ttcctaagtt ctataattcg aattagattt tttcgtaatg
    28381 atccatgttt atctggaaat actgtttcaa ctgcattttc taatgatatc gttctatcct
    28441 tactatcctt aaaatatatt gattcttcac catcattatt aatgatataa gcctttagca
    28501 ttaattccca ggcgttacag ataaaaaaac taaaaccctc aattctatac ttaattgtag
    28561 gtttgttata tatttcaatc cccataataa aagcctcaat acttttatta actaatcttt
    28621 tacttaaatc ttccatacgt ttcctctctt aaaatatttt ctatattgtg gcgcgtggta
    28681 tgctataggt cgcattttcg gcactttcgg ccatcctgtt ttgctatggt tgcttttttt
    28741 tattaagtaa ttcttcaact ttaataatat cttcttctga atgactattt ctaaaattat
    28801 taatagcttc agtatcttta gggtctatta aggcgtattc attagaaatt tttaaaattc
    28861 taatttcttc ctcaagcata gcattttctt tttcaagcat ttttagctct ttatcaattc
    28921 tcagtttttc atctaactgt gaacctaaat gagtaacgag gtcagagggt tcttttaaaa
    28981 aatcatgagg taaattatca tgctctaaat cttttataac gccttcaaaa aatttctttt
    29041 gttttctttc tgataaagat tcaaacattg taacaaaatc aatgacaggt tgctcatatt
    29101 ttaaaataac gtctgggtca ataacaaaat aacggggatc aatatctgat gcttccactt
    29161 taaaatagtc agcaagtttt tggaggtttt cggtgtttgg cattgaccta cctttgacat
    29221 aacctgttaa ggtacttttt ggaataccta aatcattatg taagtcaatt tgtcttttac
    29281 cttgttcttt cataagtcta tttaacctag cagagaaata gagtctattt tttatatctt
    29341 gagggctatt ttttgccatg tataattacc tcctcctaaa tttcctaagc ccattgtaac
    29401 acaataaaat agaaatagta cgaaaaaaat aatatttttt tattaaatga ttgacaaagt
    29461 acgaaaataa tagtacaatt caattatcat tttagaaagg attttttaaa gatggaacag
    29521 attactttaa aaggagcgcg agtttctgct ggttatacgt tgcgtgaagt tgggttaaaa
    29581 attaatagaa gttttcagac tgtcgcaaag tacgaaaaag atagtactaa aatacctatt
    29641 gatttactaa gagaattaac ttcactttat aatatttccc ctgatcttat ttttttagga
    29701 aaaagtacga aaaaaaacga acttatttag aaaggagcaa acaatggaac tagtctacat
    29761 ggacggaaag aaagagccgt atacactaag cagtattatt gcggagtgtg cagaagtcaa
    29821 gcacagacat ttgaagattt tgctaaataa acaccgagag gacttcgaga gtttcggaaa
    29881 ggtgcaattt aaaatttcac cttcagaaag tgggcaaaat gtacgggact atattttaaa
    29941 tgaacaacaa gcaacattgc tgatcactta cttacgaaat acagaacccg taaaagagtt
    30001 taagaagaac ctagtcaaag ccttctttga aatgcgtgag gaactttcta agttccgtat
    30061 gcagagggcg ctagaaaagc caaaaagaaa aacactgcat gacagtattg agaactggaa
    30121 acaagcacca aagcacgcgc atagcaccat gaacaacctg ctactaaaag cagtaaccga
    30181 caggaacgct aagcagttaa gggaagaacg tgggggctat aatggtattg atagcttgac
    30241 aagtgacgag ctggagcaat atcaagcctt tgaggatatg gtaatagcca tgattagctt
    30301 gaatatgagt tatcaggaaa tcaaggctat ggcattcaga aataaaaaaa cacgccaaag
    30361 aagcgcgtga gagcaacaaa aaaggcttag caataaccac accgcagagc ctttcacact
    30421 aacactaaaa cgaatttaac aaagcaggca agctattatc aaagaggttt tagtaaagat
    30481 ttatacctag attatagcat atttagggca atttgaccat acggagagcg ccaactcttt
    30541 aaactggtac tttctcacgc tttcaatttg gcgactctga gcgtgaggca tgtggtaaga
    30601 aaaaaagcat taaaaagccc cacgctctcg gtcggcaaac ttctgagcat gaggctgtag
    30661 gcaagaaaaa ctgtataaga aacaaccatt caaaaaaccc acactcgcca tcgcccaact
    30721 ttgagtgtga ggatatcctg gatagtaaaa ggcattaaaa agcccttagg gcaattacac
    30781 aacatagaaa cataggtaaa aaacatgaca gaaacaacat acgatattat cgctaaaagc
    30841 ttggatagaa ttagtatgga attacatcaa gcagacgaaa acaatgattt tttaagaata
    30901 ggactcttat caggacaatt aaaagctatc aaagaaaatt tacaccgctt actttggatt
    30961 gaacttcctg aattgaatga aagccataaa atcgaggcag tctctaaaag cactacggga
    31021 atgtatttcc accctggtat ttttgaaatg gacgctatgc gacaagcctt ctttaaacgc
    31081 caagccaagc acttttttga caacgaagca gagcaacagg cgtatataga acatgctgaa
    31141 aaggagtatt tagaggctac tataacctta aaagatattc tttttaattt taaaaatgct
    31201 aataggaaag taagtaaaga tcatcttata gagaagtttg aggaggcgat gaaatgaatg
    31261 agctagattt aaccaataca caggcgctaa tcttcatggt ggtactgatt ggcttactgc
    31321 tttatctaaa ccaccgagac cgcaaaaaaa gcgccaaaat agagcgagaa agccaacaga
    31381 cgatagaaac acctagcgag gatttaaacc cttattatgg gcgttatatc cagcttgcag
    31441 gggtaagagt ttacggagga actgaatgag tctatcagag aatgacaaac gagtattaag
    31501 actaatcaag gtaggggctg agaactccat aacaggggca gaaatcagcc tgatcactaa
    31561 gctggcagaa agaaccgtac aggacattat cagccgtcta atcacgcgca atagcattcc
    31621 tattgttggg gttcgacacg ggacatttag aggatatttt atcccagcta acaaagagga
    31681 gctattggac agtgcaaaaa ccttttacaa tcagatacga gaagaagaaa agcgcctagt
    31741 ggtgttgatg aacagcgacc tagaaagcta caaagaaacc ttgaaggagg tgggcggata
    31801 tgtttagcct aagccgagaa agcgagcaag acctagcgca tggcattata gaggtagtgg
    31861 aaagatacct ggaagcgcgt gatacagtca aaccacgact gacaggttta atctcagccc
    31921 aggaagtcat gggcgagtta gatataaaat atctgacact ccaaaagtgg gagaaagcag
    31981 ggctaaggcg ataccatcca ccgcttgaag atacaagaaa ggtttattat aaaataactg
    32041 acatctggaa gtttatgggg gtggagaatg gcaaaaacta aaatatattt ttggctaaaa
    32101 attgataaga aattttttga taatattttt atcaagagac taaaaacggt acctggtggg
    32161 tacactatga cggtgattta tattaggctc atgctagaga gcttagaaag tgactgtatt
    32221 ttatactatg agggttactt tgacaatctc aaggaagagt tagccctaaa attagatgta
    32281 tcggaagatg atatagatat gaccatggca tactttacta agtgtggtct aatacaagtt
    32341 gatgaagata aaaacgcaga actaccgcag gcaaaagcct tggtacagca ggaaaccaac
    32401 caagcagcat acatgcgaga atataggaaa cagcaacgag aacaaaagga aaaccttaca
    32461 atgtcatctg agaatcttac attgttaact acatgtaaga cagagataga gaaagagata
    32521 gagaaagagt tagaagaaga gatagagaaa aaaacttctt ctgctcctac tgctgaaatc
    32581 tctaactatt accaaaagcg tataggggtt atggatgggc aacaatatca aatactgact
    32641 gactatctca ctcttgacgg tatggagcta gaggttatca agattgctat agacaaggct
    32701 gcagataatg gcaagcgatc ttttagctat atcaattcaa tactcaagaa ctggcgacag
    32761 aatggtatta gaaccatggt acaagtcgaa gatgaacaga gactgtttca gcaaaagaaa
    32821 caaggtcagt ctgacgatga tattcaagac ccgtttatct actgacggag aggagaaaca
    32881 atggaattac tgaatacaga ccagctcaat gaaaaaataa aggtcactga tgagctttgc
    32941 ccaatccatc agattaacct agtacaattc aaaaagccag atggttatac atctccctac
    33001 tgtatggaat gcactaggga gaacatcaaa cagacggagc tagagggcat agaaaaagcc
    33061 ctaggccatg atctggactc ttcaacctat gatgtgctag tgcgtgaaag tacggtgtca
    33121 aatgagctga gagaagccac gtttagcact ttcaaagccg agacgcaaga ggaacacgag
    33181 gccaaggagt ttgctatcaa gcaagctaag cagtacctaa acggtatgac aggaaacacg
    33241 ctgattatgg gcaatccagg tactggcaaa agtcatttat gctattctat ggctaaagcc
    33301 attaacgagg gctataagtc taggaatgat cctaagagtg tgctttttgt cagtattact
    33361 gagattatca cgcgcattca gtcaggttgg cagtatagac agagtgattt tacagagtat
    33421 gacgctttga agttgctgac tgaggttgac tatctcttta ttgatgattt aggaacagaa
    33481 agcgttatga acagccaaaa aaatgaagct aataattggg tacaggcctt tcttttcaag
    33541 atttttgata agcgagacac aactatcatc aacaccaatc acaacggaaa agagctggcc
    33601 agaatctata atgataagct agtcagtcgc attggaaaac aatcagaggg gaacgtattt
    33661 attattacag acattaaaga taagaggatg aagcgcaatt tttagccgga gaaaaaggag
    33721 aaaaacaaca tgaaagtcaa gctaaacgac ttcatggcaa ctgttgaagt gctggacgtt
    33781 aaattttctg gtacaccatc tagtgacacc cttaaaacag aaagaggaaa aacaacatga
    33841 cattaaaaac attttcggat aaagccaaaa catttacttt cacttacgaa tttaaagacc
    33901 tagatactgc tctggtagca ggtcacgcgc tactgggata tatgactgga acatattgcc
    33961 agcctacaat ctcattgacc cacaaggata aaggcactct tgtagctgag tatgtggagg
    34021 ataacaaact aaacaagacc ttcaagcgta tttgtgacag ttttaaagac tactacaacc
    34081 aaccagtgga cgatgaagca tttgaagaac gttacaaacg ggagcgcgtg cttcaactca
    34141 aagagtcaga ggattttgat agtttgctga ataaggtcac agactacgag ctggaactat
    34201 tagactatgc tgatcgcttg ctttctgata agcctatccc tatggactct atgaccgctt
    34261 ttggtacttt ggaaatgctg ggtaatgaaa gtattaacct gctccaaaac ttggacgtag
    34321 aaggcgaata taaaggcctt gctgattatg tggcagagcc agaaagctag aactatcaag
    34381 agaggcacta gcctcttttt gtggtttcat tgctacctaa cccaatcgat ttgggttttt
    34441 tacatgataa tttgtagtag tgtgcaattt acaaaacaaa caaatactac atatttgacg
    34501 gtttgaatgt taaaaaagct agatatatca aggatttaag taagtagagt gttccaaaaa
    34561 agggtgcagt aagcgttcca aaaaaagggt gagataaggg tgaggttgaa tatataaaaa
    34621 gtataagatt agtataaggt tgtgtattta gaaatacaaa gtataggcta agattaggct
    34681 aaggtcacat acttcaaaat actaaggttt tacaaaggta cagggctttt tttctagtga
    34741 tagaacccca aggttaccac aaggtatagc tactttttta gaaccataga acggtaaggt
    34801 tacggtaagg tatcaaagtt ttccaatggt gaggttttgg tgaggtaaga gcaaaaaatg
    34861 taaaggtttt gtaaaggttg cgtatttgaa agccccaagg ttaccacaag gtagagaggt
    34921 ttaccgaggg ttagaaaagg tgagattttg cttaggtagt ggcagtaaat atgcctgaca
    34981 aatggcaggg ttatgtgtgt tgaactccga gcgaacgccg agaaagtgga tatatcccct
    35041 atcatttatc aagcagataa gagaaataat aaccctcctt aactccctga gaaacgacaa
    35101 gcagataagc ggagttttta cccctcttat ttccgattta ctccttgcag aatgataggc
    35161 agataaggac gacaataagc cttcttaatt gcttacaaaa cgatagggag ttaagacgaa
    35221 tttaaaagct ctttattttc gtttttctcc ttacaaaatg acagggaaat aagaactaaa
    35281 ataactctcc ttattttcct accaaatgat aaggagataa gagcaattta aaagtctgtt
    35341 attttggatt tgttgcttac caaatgataa gtagatcagc aaaggaataa cttgccttaa
    35401 ttgcttgcag aatgacaagg aaataagaga gataaaaaca tcccttaatt gcttatcaaa
    35461 tggtacgtag aatggacttg aacctagagc gaagctagag aaaatgaaaa aaagccagca
    35521 cacggctgac tcctttatga attattccct aaaactatta tatcacaaag gaggccgagg
    35581 catgacacca gagcaggtaa aagaaaaact agagggcgtt aagtggataa ataaggagat
    35641 caaaggctta tatttggaat tggaagccct ggaaggtggc attatccaaa agccaacact
    35701 aagccatagc agggtgcaga caagcagaga gaacaagaca gagaacaatt ttataagtgt
    35761 tctgaagcta aaagaggata cgcttcagag gattgagcgg cttactgagg aaagaatgga
    35821 aatatctggg ctgatcgata agctagccaa tccgcttgag cgctctgttc tgagactttt
    35881 ttacttgaat gatctcgata tttggggagt tgctgaggaa ataggtaaat ctaaagcttc
    35941 gatatatcgg gcaaagaagg cagctataga acacttggta tgtatggtaa atgggaattg
    36001 atttaacctt accacagagc ctagtgcgta tagggggcac taggctttat attcgcccct
    36061 tattcgtttt gttttggact ggataactaa atcaccacgg ataaactaca agccctcaga
    36121 aacaaatcta ggggcttttg tgagataaaa aaataaaggt gccccaccgc ctccacagca
    36181 agctgcttca tgggcgttag accaccttta ccttaactta attataactc acaatgcgga
    36241 gtttttcaag aaaggataag agactagaaa actagctact tattggaaca acaaagctag
    36301 tgagctattg aagaacggta agattttggt tagatacaga gaaacaggcc taagcctgct
    36361 ctaaatctaa cttaatttct tcacaaacta ttaaattcaa ataagctgag aaaacataaa
    36421 gggtaacaaa aaggggactt aaattgaaga gttcagtaag caagaaacta acacaatcaa
    36481 acgtgctttt ttttgtaaaa atgttgtata cttaagtaaa taaaggaact ggtttggcac
    36541 ccaatggctt attttaggtc ggcgcctaag tcagttcctt tttactgttt cgaccacccc
    36601 aaaggatatg cctctaacat tattgcgtca attagttatt taggctataa ttaaattaac
    36661 gaatagaggt tttccatctc cccttgaaac agttcattcc gcggtaggag gtgggacgcc
    36721 tctattttta ccataaagga gtatttaaca tggcaaaagt taaagaaaaa tggaacccaa
    36781 caatatccca tattgtccct aaaggtacca aactatcaga cggtacaata ttagacaaag
    36841 aaaccacctt aacacaagaa gaatttacta aaaatccccc tgttattcca gcaggacacc
    36901 cttattataa tcttttagct agaataagca gagaggaaat agaaaaggaa gatctatagt
    36961 acaaagaaat agtaaaatat aatagttttg cgcgtgatgt tcgcttgaaa aggacttgtt
    37021 aaggtcaaca aaatcgataa ccacgcgctt ttatctatct caccttttcg atagataaaa
    37081 caaccaatac gcgtgatgaa catggtataa tatattcaca gctggaggct tgcacagtct
    37141 agccgattag ggagagttag accgagatgt cacacactcc cttttttggt ataataaacc
    37201 tatcagataa gcaaaaacaa ctaaaaaagc gtagctgatg actacgctag tttcttgcct
    37261 gctgaactcg tcaaatctta gcccttttgt ggggcttttt tgctcccctt tttgcgtact
    37321 ttgggggagc aagtagtaaa aagtaatatt agtgtttttt gttaaaaagc gcgtgatata
    37381 agggttagga agccctaagg aaacataaaa taagaagttt tttattatac tacaaaatcg
    37441 gccttttggg taatagaatc tcactttttt tggtataatg gtaagcaata atggactaga
    37501 aagaacaaag atgcaagata aaattgtcat tcatggggcg cgtgcccata atttaaaaaa
    37561 tattgatgtg gagattccgc gagacaagtt ggttgtcgtg actggcttgt caggttcagg
    37621 gaagtccagt ctggcttttg atactctcta tgcggaggga caacgtcgct atgtagagag
    37681 tttgtccgcc tacgctcgtc agttcttggg aaatatggaa aaaccagatg tagatgccat
    37741 tgatggtctc agcccagcta tttccatcga ccagaaaacg actagtaaaa atcctcgctc
    37801 gacggtggga acgacgactg aaatcaatga ctatctgcgt ctcctctacg cacgtgtggg
    37861 gacgccttac tgtatcaacg gacatggagc tatcaatgct tcttctgtgg agcaaatcgt
    37921 tgataaggtt ttggagttac ctgaacgcca gcgcttgcag atcttggctc ctgtcatccg
    37981 caagaaaaaa ggccaacata agagtgttat cgagaaggtt cagaaagacg ggtatgttcg
    38041 tgttcgtgtg gatggggaag tctatgatgt gaccgaagtg ccagagttgt ctaagagcaa
    38101 gcaacacaat atcgatgtcg tggttgaccg tattgttatc aaggagggca ttcgtagtcg
    38161 tctctttgat tccattgagg ctgcccttcg tattgcagaa ggttatgtca ttatcgacac
    38221 catggacgat tcggagttgc tcttctctga gcattatgcc tgtccagttt gtggttttac
    38281 tgtcccagag ttagagcctc gtctcttctc tttcaatgct ccttttggct cttgtagtga
    38341 gtgtgacggc ttgggcatca agctggaggt ggatactgat ttggtagtgc cagatgccag
    38401 caaaacctta catgagggag cgctggcacc ttggaatcct atctcatcca actactatcc
    38461 aaacatgtta gagcaggcca tgaaagtctt tggagtggct atggataagc cttttgagga
    38521 cctgtcagaa gaagataaga acttgattct ctacggctca gatggtaagg aattccattt
    38581 ccattatgaa aatgaatttg gtggtgtgcg cgatatcgac attccgtttg agggagttat
    38641 caataatatc aaacgtcgtt accatgaaac caatagcgat tacactcgca ctcagatgcg
    38701 tctctacatg aatgagctga cctgcggaac ctgtcaaggc tatcgtctca atgaccaggc
    38761 cttgtctgtc cgtgtgggcg gccagcaagg gccacatatt ggagaaatct cagacctgtc
    38821 tatcgctgac cacttggact tggtgagcca gttaactttg tctgaaaatg aagccatcat
    38881 tgctcgtccc attctcaagg aaatcaagga tcgtttgacc ttccttaata atgtgggtct
    38941 taactatcta accctgtcac gttcagcagg aaccctttca ggtggggaaa gtcagcgcat
    39001 tcgtttggca acccagattg gttccaacct atcaggtgtc ctctatatcc tagacgagcc
    39061 gtcaatcggt cttcaccaga gggacaatga ccgcctgatt gccagtctga aaaagatgcg
    39121 tgacttgggc aatactctta tcgtggtgga acacgacgaa gataccatgc gtgaagctga
    39181 ttatctgatt gacgttggtc ctggtgccgg tgtttttggt ggtgagattg ttgcagcagg
    39241 tacacctaag caagtggctc gcaacagcaa gtctatcaca ggtcagtact tgtcaggcaa
    39301 acgtgtcatc ccagtaccag aagagcgccg tgtcggaaat ggtcgtttta ttgaagtgac
    39361 aggagcgcgt gagaacaact tgcaaaatgt cacggctcgc tttccactag gaaaatttat
    39421 cgcagtgaca ggtgtgtcgg gttcagggaa atcgactcta atcaacagca ttctcaaaaa
    39481 agccattgcc cagaagctca accgtaattc agacaaacct ggtaagttta agactattac
    39541 agggattgag catgtcgacc gtttgattga tattgaccag agccctatcg gacggacgcc
    39601 gaggtctaac ccagctacct atacaggagt ttttgacgat atacgtgacc tttttgctca
    39661 gacaaatgaa gccaagattc gaggctacaa gaaggggcgc ttcagtttca acgtcaaggg
    39721 aggtcgctgt gaagcttgct caggtgacgg gattatcaag attgagatgc acttcttgcc
    39781 agatgtttat gtggcttgtg aagtttgcca cggaacccgc tataacagtg aaaccctaga
    39841 agttcactac aaggaaaaga atatttcgca ggtcttggat atgacggtca acgatgcggt
    39901 agaatttttc caacacattc cgaaaattca acgcaaactc cagaccatca aggatgtagg
    39961 tctaggctat gtgaccttgg gacagccagc taccaccctt tctgggggag aagcccaacg
    40021 tatgaagtta gctagcgaac tccacaaacg ctcgacagga aaatctttct acattctgga
    40081 tgagccgacg acagggcttc atacagagga cattgctcgc ctgctcaagg ttttagctcg
    40141 ctttgtagac gacggcaata cagtcctcgt catcgagcac aatctggatg ttatcaagac
    40201 ggcagaccat atcattgact tgggacctga gggcggtgtc ggtggtggaa ccatcatcgt
    40261 aacaggaact ccagaagaag tagctgccaa cgaagccagc tatacaggac actatttgaa
    40321 aggaaagtta catcatgaat aaacgcgtac aagcatttct agctaaaata caagaaaaag
    40381 aactagatgg tatcatcatc aacaatctta aaaacgtcta ttatttgact ggtttttggg
    40441 gctcaaacgg aacagtcttt atcagccgtg accgtcaggt cttagtgaca gactctcgct
    40501 atatcatcgc agctaagcaa gaaaccagtg gttttgagat tgtggctgat cgtgatgaat
    40561 tggctgtcat tgcaggaatt gttaaggaca tgggcttgac tcgtatcggt tttgaagatg
    40621 agatttcagt gtcttattac caccgtatgc aggcagcttt tgcaggtttg aacttgcttc
    40681 cacaaactca gtttgtggaa ggtcttcgta tgattaagga tgaggcagag attgcagcga
    40741 ttcgcaaggc ttgttctatc tcagaccaag ctttccgcga tgcgcttgac tttatcaaac
    40801 caggaaaaac tgaaattgag attgccaact tccttgattt ccgcatgcgt gagttgggag
    40861 catctggctt atcttttgat acgatcctag ctagcggtat caattcttct aaaccccatg
    40921 cccatccaat gcacaaacca gtggagttgg gagaagccat taccatggac ttcggctgtc
    40981 tctatgacca ctatgtcagt gatatgaccc ggactatcta tctagggcat gttagcgatg
    41041 agcaggcaga gatttacaat acggttctaa aagctaacca agccttgatt gaccaagcta
    41101 aggcaggctt aggtttccgt gactttgaca aaatccctcg tgatattatc attgaggcag
    41161 gttatggtga ctactttact cacggcattg gccacggtat tggtctggat atccatgagg
    41221 aaccctactt tagtcagact tctacagaaa ctattaagac aggtatggcc ttgaccgatg
    41281 aaccaggtat ctatatcgaa ggcaaatatg gcgttcgtat cgaggatgat atcctgatta
    41341 cagagacagg ttgtgaatta ttgaccctag ctccaaaaga gttgatagtc atttagttgt
    41401 ttgtcaagaa aaaagtacga aaatgacgaa aaaagtcaaa aaaaattaaa a
//
LOCUS       DBERMR010000013        39746 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.013, whole genome shotgun sequence.
ACCESSION   DBERMR010000013 DBERMR010000000
VERSION     DBERMR010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 39746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39746
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.013"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            9..89
                     /locus_tag="ACL8A1_000744"
     tRNA            9..89
                     /locus_tag="ACL8A1_000744"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43..45,aa:Tyr,seq:gta)
     gene            94..164
                     /locus_tag="ACL8A1_000745"
     tRNA            94..164
                     /locus_tag="ACL8A1_000745"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:126..128,aa:Trp,seq:cca)
     gene            179..251
                     /locus_tag="ACL8A1_000746"
     tRNA            179..251
                     /locus_tag="ACL8A1_000746"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:212..214,aa:His,seq:gtg)
     gene            265..336
                     /locus_tag="ACL8A1_000747"
     tRNA            265..336
                     /locus_tag="ACL8A1_000747"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:297..299,aa:Gln,seq:ttg)
     gene            346..429
                     /locus_tag="ACL8A1_000748"
     tRNA            346..429
                     /locus_tag="ACL8A1_000748"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:380..382,aa:Leu,seq:caa)
     gene            536..1813
                     /locus_tag="ACL8A1_000749"
     CDS             536..1813
                     /locus_tag="ACL8A1_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HGS1201551.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATPEIQAFALNGDEVIISPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            1889..3373
                     /gene="thrC"
                     /locus_tag="ACL8A1_000750"
     CDS             1889..3373
                     /gene="thrC"
                     /locus_tag="ACL8A1_000750"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HGS1201552.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLDF
                     DKLKDTSYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKDGVSKIQELQMTTQTGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTVELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVCELDS
                     YIEDPHTAVASAVYKKYQSATGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            3434..4714
                     /locus_tag="ACL8A1_000751"
     CDS             3434..4714
                     /locus_tag="ACL8A1_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HGS1201553.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTILSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLLGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            4718..5338
                     /locus_tag="ACL8A1_000752"
     CDS             4718..5338
                     /locus_tag="ACL8A1_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGS1201554.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTAILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            5504..6646
                     /locus_tag="ACL8A1_000753"
     CDS             5504..6646
                     /locus_tag="ACL8A1_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HGS1201555.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEAPKEVASSSEVTKTV
                     IASEEVAPSTGTSVPEEQTAETSSAVAEEAPQETTPAEKQETQASPQAASAVEATTTS
                     SEAKEVASSNGATAAVSTYQPEETKVISTTYEAPAAPDYAGLAVAKSENAGLQPQTAA
                     FKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGI
                     SYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            6969..7394
                     /locus_tag="ACL8A1_000754"
     CDS             6969..7394
                     /locus_tag="ACL8A1_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGS1201556.1"
                     /translation="MLEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAIQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIKVKEEMESILTEFERTELSRLLNKLVLGIENIEI"
     gene            7405..7806
                     /locus_tag="ACL8A1_000755"
     CDS             7405..7806
                     /locus_tag="ACL8A1_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HGS1201557.1"
                     /translation="MSIITIILTTIVALEHFYIFYLESIATRSDATSRVFNMEKEELA
                     HPSVSSLFKNQGIYKALLGVFLWYGIYFSQNLEIVTIFVLFVIGAATYGSLTADKKII
                     LKQGGSAILALISILLFKIHLKVDSNLANPF"
     gene            7881..8525
                     /gene="pcp"
                     /locus_tag="ACL8A1_000756"
     CDS             7881..8525
                     /gene="pcp"
                     /locus_tag="ACL8A1_000756"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HGS1201558.1"
                     /translation="MKVLVTGFEPFGGEKGNPTLEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSVTPERVAINQDDARISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKFPYVK
                     AGFMHIPYMMEQVVNRPTTPTMSLVDIRRGIEAAIGAMIEHGDQELKLVGGETH"
     gene            complement(8553..9410)
                     /locus_tag="ACL8A1_000757"
     CDS             complement(8553..9410)
                     /locus_tag="ACL8A1_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HGS1201559.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSSFLNP
                     LSRIHGTVLEQPFFSILGQILQAYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            9540..10682
                     /gene="tgt"
                     /locus_tag="ACL8A1_000758"
     CDS             9540..10682
                     /gene="tgt"
                     /locus_tag="ACL8A1_000758"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HGS1201560.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            10783..12600
                     /locus_tag="ACL8A1_000759"
     CDS             10783..12600
                     /locus_tag="ACL8A1_000759"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HGS1201561.1"
                     /translation="MRIKWFSLVRITGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNHEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     ASVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAVAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPIVQTLRPLPYIHAILAAGTGILTIIVCTVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            12753..13904
                     /gene="nagA"
                     /locus_tag="ACL8A1_000760"
     CDS             12753..13904
                     /gene="nagA"
                     /locus_tag="ACL8A1_000760"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HGS1201562.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            14067..15125
                     /locus_tag="ACL8A1_000761"
     CDS             14067..15125
                     /locus_tag="ACL8A1_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HGS1201563.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLINGMQAEYLRVPHADNTLYHTPADLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETAVSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPATFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            15276..15641
                     /locus_tag="ACL8A1_000762"
     CDS             15276..15641
                     /locus_tag="ACL8A1_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HGS1201564.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            15717..16658
                     /gene="comGA"
                     /locus_tag="ACL8A1_000763"
     CDS             15717..16658
                     /gene="comGA"
                     /locus_tag="ACL8A1_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HGS1201565.1"
                     /translation="MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKIASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            16606..17622
                     /gene="comGB"
                     /locus_tag="ACL8A1_000764"
     CDS             16606..17622
                     /gene="comGB"
                     /locus_tag="ACL8A1_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HGS1201566.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSALLDKQCVTQMRVGLSQGKSFSEMMESLGCSSAIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
                     LGMIVFVSVGVLLALTFYKRSSKMRVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLRPMYQNM
                     EVNF"
     gene            17624..17950
                     /gene="comGC"
                     /locus_tag="ACL8A1_000765"
     CDS             17624..17950
                     /gene="comGC"
                     /locus_tag="ACL8A1_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="comG operon protein ComGC"
                     /protein_id="HGS1201567.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLEKNEDASLSKLKDDGRITEEQVKAYKEYNDKNGGA
                     NRKVND"
     gene            17943..18347
                     /gene="comGD"
                     /locus_tag="ACL8A1_000766"
     CDS             17943..18347
                     /gene="comGD"
                     /locus_tag="ACL8A1_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HGS1201568.1"
                     /translation="MIKAFTMLESLLVLGLVSILALGLSGSVQSTFAAVEEQIFFMEF
                     EELYRETQKRSVASQQKTSLNLDGQTISNGSQKLPVPKGIQVPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            18310..18612
                     /gene="comGE"
                     /locus_tag="ACL8A1_000767"
     CDS             18310..18612
                     /gene="comGE"
                     /locus_tag="ACL8A1_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGE"
                     /protein_id="HGS1201569.1"
                     /translation="MEKLNALRKQKIRAVILLEAVVALAIFASIATLLLGQIQKNRQE
                     EAKILQKEEVLRVAKMALQTGQNQVNINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            18575..19036
                     /gene="comGF"
                     /locus_tag="ACL8A1_000768"
     CDS             18575..19036
                     /gene="comGF"
                     /locus_tag="ACL8A1_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HGS1201570.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALLVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWILFVDQLEAELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            19014..19460
                     /gene="comGG"
                     /locus_tag="ACL8A1_000769"
     CDS             19014..19460
                     /gene="comGG"
                     /locus_tag="ACL8A1_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HGS1201571.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLNRQIAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTSKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKAEKKNQKRSLKRKRIPSQFNYNALNPE"
     gene            19563..20150
                     /locus_tag="ACL8A1_000770"
     CDS             19563..20150
                     /locus_tag="ACL8A1_000770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGS1201572.1"
                     /translation="MKHDFNHKAETFDSPKNIFLANLVCQAVEKQIDILSDKEILDFG
                     GGTGLLVLPLAKQAKSVTLVDISEKMLEQARLKVEQQAIKNIQFLEQDLPKNPLEKEF
                     DCLAVSRVLHHMPDLDAALSLFHQHLKEDGKLIIADFTKTEANHHGFDLAELENKLIE
                     HGFSSVHSQILYSAEDLFQGNHSEFFLTVAQKSLA"
     gene            20211..21164
                     /locus_tag="ACL8A1_000771"
     CDS             20211..21164
                     /locus_tag="ACL8A1_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGS1201573.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEMDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKEWLKEEASLVAMISLPENLFANTKQSKTIFI
                     LQKKNEIAVDPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            21215..22405
                     /locus_tag="ACL8A1_000772"
     CDS             21215..22405
                     /locus_tag="ACL8A1_000772"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HGS1201574.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKYIGQYLAVLNGADAIVFTAGVGE
                     NAENFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            22406..22537
                     /locus_tag="ACL8A1_000773"
     CDS             22406..22537
                     /locus_tag="ACL8A1_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201575.1"
                     /translation="MKLKKYSIQGVGKVIFPASFSDEIVQNLAMIGFFEKYGIIVVI"
     gene            22554..22925
                     /gene="rnpA"
                     /locus_tag="ACL8A1_000774"
     CDS             22554..22925
                     /gene="rnpA"
                     /locus_tag="ACL8A1_000774"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HGS1201576.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            22900..23724
                     /locus_tag="ACL8A1_000775"
     CDS             22900..23724
                     /locus_tag="ACL8A1_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HGS1201577.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYS
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            23743..24729
                     /gene="jag"
                     /locus_tag="ACL8A1_000776"
     CDS             23743..24729
                     /gene="jag"
                     /locus_tag="ACL8A1_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HGS1201578.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERYASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQTIIDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNCYSRTFYITINVNDYVEHRAEVLQTYAQKL
                     AIRVLEEGRSHQTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            24780..25403
                     /locus_tag="ACL8A1_000777"
     CDS             24780..25403
                     /locus_tag="ACL8A1_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI_0552 family protein"
                     /protein_id="HGS1201579.1"
                     /translation="MLNKIRDYLDFSGLQYRSPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            25534..26991
                     /locus_tag="ACL8A1_000778"
     CDS             25534..26991
                     /locus_tag="ACL8A1_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGS1201580.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIVAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            27014..27295
                     /locus_tag="ACL8A1_000779"
     CDS             27014..27295
                     /locus_tag="ACL8A1_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS1201581.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            27372..27857
                     /locus_tag="ACL8A1_000780"
     CDS             27372..27857
                     /locus_tag="ACL8A1_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002267837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGS1201582.1"
                     /translation="MNLKQALIDNDSIRLGLEANDWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            27874..28539
                     /locus_tag="ACL8A1_000781"
     CDS             27874..28539
                     /locus_tag="ACL8A1_000781"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HGS1201583.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            28543..29406
                     /locus_tag="ACL8A1_000782"
     CDS             28543..29406
                     /locus_tag="ACL8A1_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HGS1201584.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKAIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIS
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAVQEAQAF
                     LYPLIKKAGLM"
     gene            29408..30112
                     /locus_tag="ACL8A1_000783"
     CDS             29408..30112
                     /locus_tag="ACL8A1_000783"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HGS1201585.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            30293..31966
                     /locus_tag="ACL8A1_000784"
     CDS             30293..31966
                     /locus_tag="ACL8A1_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGS1201586.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALVDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNESKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            32078..33169
                     /gene="ulaG"
                     /locus_tag="ACL8A1_000785"
     CDS             32078..33169
                     /gene="ulaG"
                     /locus_tag="ACL8A1_000785"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HGS1201587.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            33283..35259
                     /gene="tkt"
                     /locus_tag="ACL8A1_000786"
     CDS             33283..35259
                     /gene="tkt"
                     /locus_tag="ACL8A1_000786"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGS1201588.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDTNRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            35376..35675
                     /gene="yajC"
                     /locus_tag="ACL8A1_000787"
     CDS             35376..35675
                     /gene="yajC"
                     /locus_tag="ACL8A1_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HGS1201589.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            35722..36150
                     /locus_tag="ACL8A1_000788"
     CDS             35722..36150
                     /locus_tag="ACL8A1_000788"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HGS1201590.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            36152..36562
                     /locus_tag="ACL8A1_000789"
     CDS             36152..36562
                     /locus_tag="ACL8A1_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HGS1201591.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            36862..39513
                     /gene="adhE"
                     /locus_tag="ACL8A1_000790"
                     /gene_synonym="adhC"
     CDS             36862..39513
                     /gene="adhE"
                     /locus_tag="ACL8A1_000790"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HGS1201592.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT        12103 a         7085 c         8945 g        11613 t
ORIGIN      
        1 tatattttgg aagggtagcg aagaggctaa acgcggcgga ctgtaaatcc gctccttcgg
       61 gttcgggggt tcgaatccct ccccttccat tttacgggca tagtttaaag gtagaactaa
      121 ggtctccaaa accttcagtg tgggttcaat tcctactgcc cgtgttaata gaattatggc
      181 gggtgtggtg aagtggttaa cacaccagat tgtggctctg gcatgcgtgg gttcgatccc
      241 catcactcgc ctattttata ttattggggt atagccaagc ggtaaggcaa gggactttga
      301 ctccctcatg cgttggttcg aatccagcta ccccagttac tatttgccgg cgtggcggaa
      361 ttggcagacg cgctggactc aaaatccagt gtccgcaagg acgtgccggt tcgaccccgg
      421 ccgccggtat agtatagtgt taggaacgtt gttattcttc gttccttttt tatattattt
      481 ttggtataat tatagttatt caaattttat ttagattaag aaagtgtagg gtagtatgtc
      541 ttgttctatc gatttattaa aacatcggta tttgaaaaat attaaagaaa atcctgaatt
      601 gtttgtcgga attgagttgg agtatcctgt tgcaagttta gaaggggatg ctacagatgt
      661 tgaagttata aaggatctat ttcattattt agtttctact ttggatctca ccgtagcaaa
      721 ggtagatgat tttggcaatc tgatccagtt agtagatccg ataagtcagg atgctatttt
      781 atttgaagtt tcctatacaa cgattgagtt tgcatttggt aaggctgaaa cgattcaaga
      841 ggtcgaaaat cgtttcaata attatatgaa tgtaattcag agaaagttag ctgaatcaaa
      901 tcatgctatt gttggctgtg gtatccatcc caactgggat aaaaatgaga attgtccagt
      961 ggcttatcca cgctatcaga tgttgatgga ttatttgaat ttgagtagaa atattattaa
     1021 atcagattta catcatttcc ctgaatatgg tacttttatc tgtgggagcc aggttcagct
     1081 ggatatttca aaaaccaact acttacgggt gattaatgct tttactcaaa ttgaagcggc
     1141 taaggcttat ttatttgcaa actctgaatt ttcgggtgcg gattgggata cgaaaatttc
     1201 aagggatatt ttctgggaag aatctatgca tggtatctat ccagagaatg ttggggtcaa
     1261 tgctagactc cttaatgatg aagctgattt ttttgactat ctaaatcatt ctgcgatttt
     1321 tactgcggaa cgtgatgggc agacctatta tttttatcct attcaggctg gggactattt
     1381 ggctacgcct gaaatccaag catttgctct gaatggggat gaggttatta tttcccccca
     1441 agagaaggat tttgaaactc atcgtagtta ccagtaccaa gacttaacga ctcgaggaac
     1501 agttgagttt cgtagtgtgt gtacacagcc acttgatagg acttttgctt ctgcagcttt
     1561 tcacttggga ttattggtta atttagacaa gttagaagct tacttagaaa cagcaccttt
     1621 ctttaaagta tttggttatg attacaagtc tttaaggaga caattttcta agaaaaatct
     1681 tacagatgag gaagaaacta cgattattga attttccaaa gacttactcc tactagctga
     1741 ggagggacta gtggtgagaa ataaggaaga aatgacctat ttacagcctt tgagagaaga
     1801 attgagccta taatttctct tataaaggga gaattttctg aaaaatcatg atataatgga
     1861 cgagactata gataaaggat agagagtaat gacattagtt tatcaatcaa cgcgtgatgc
     1921 caacaataca gtaactgcta gccaagcaat tttgcaaggt ttggcgacgg acggcggttt
     1981 gtttacaccg gttacttatc caaaggtaga tttggacttt gacaaattga aagatacttc
     2041 ttaccaggaa gttgctaagc tagttttgtc agcattttta gatgacttta cagctgagga
     2101 gttggactac tgtatcaaca atgcctacga tagcaaattt gatactccag ctattgcacc
     2161 attagtgaaa ttagatgggc aatacaattt ggaacttttc catggttcaa cgattgcctt
     2221 taaggatatg gccttgtcta ttttgccata ctttatgacg actgctgcta agaaacatgg
     2281 tttggagaac aagattgtta tcttgacagc gacatctggt gacacgggga aagctgctat
     2341 ggcggggttt gcggatgtgc ctggtactga gattatcgtc ttttatccaa aggatggtgt
     2401 cagcaagatt caagagttac aaatgaccac tcaaactggc gacaatactc atgttattgc
     2461 tattgatggt aactttgacg atgcgcaaac aaatgtgaag cacatgttta acgacgtggc
     2521 tcttcgtgaa aaattgacta ccaacaagtt gcaattttca tcagctaact ctatgaacat
     2581 tggtcgtctg gtgccacaaa ttgtttatta tgtttatgct tacgctcaat tggttaagac
     2641 tggtgaaatt gtagctggtg aaaaggttaa cttcacagta ccaacaggaa actttggaaa
     2701 tatcttggct gccttttatg ccaaacaaat tggtttgcca gttggtaaat taatctgtgc
     2761 ttcaaatgac aataatgtct tgacagactt ctttaaaaca cgtgtctatg acaaaaaacg
     2821 tgagtttaag gtaacaacca gcccatctat ggatatcttg gtatcttcaa acttggagcg
     2881 cttgattttc catcttttgg gaaataatgc tgaaaagaca gttgaactta tgaatgcctt
     2941 gaacacgcaa ggacaatata agttgacaga ctttgatgca gagattttgg acctctttgc
     3001 agctgaatat gcgactgagg aagaaacggc agcagagatc aagcgtgttt gtgagttaga
     3061 ttcttatatc gaggaccctc atacagctgt tgcttcagca gtttataaaa aataccaatc
     3121 ggccactgga gatgtaacta agacagtgat tgcttcaaca gctagtccat acaagttccc
     3181 agtagttgca gtagaagctg taactggaaa agcaggttta acagactttg aagccttggc
     3241 tcaattacat gaaatctcag gcgttgcagt gccaccagca gttgatgggc ttgaaatagc
     3301 tccaattcgt cacaagacaa cagtggcagc tgctgacatg caagcagcgg ttgaggctta
     3361 tttaggactt taagacagag ggagcaaact cggttgggaa accaactgag tttcttttca
     3421 tcaggaggag agattgttta agaaaaataa agacattctt aatattgcat tgccagctat
     3481 gggtgaaaac tttttgcaga tgctaatggg aatggtggac agttatttgg ttgctcattt
     3541 aggattgata gctatttcag gggtttcagt agctggtaat attatcacca tttatcaggc
     3601 gattttcatc gctctgggag ctgctatttc cagtgttatt tcaaaaagca tagggcagaa
     3661 agaccagtcg aagttggcct atcatgtgac tgaggcgttg aagattacct tactattaag
     3721 tttcctttta ggatttttgt ccatcttcgc tgggaaagag atgataggac ttttggggac
     3781 ggagagggat gtagctgaga gtggtggact gtatctatct ttggtaggcg gatcgattgt
     3841 tctcttaggt ttaatgacta gtctgggagc cttgattcgt gcaacgcata atccacgttt
     3901 gcctctctat gttagttttt tatccaatgc cttgaatatt cttttttcaa gtctagctat
     3961 ttttgttctg gatatgggga tagctggtgt tgcttggggg acaattctgt ctcgtttggt
     4021 tggtcttgtg attttgtggt cacaattaaa actgccttat gggaagccaa cttttggttt
     4081 agataaggaa ctgttgacct tggctttacc agcagctgga gagcgactta tgatgagggc
     4141 tggagatgta gtgatcattg ccttggtcgt ttcttttggg acggaggcag ttgctgggaa
     4201 tgcaatcgga gaagtcttga cccagtttaa ctatatgcct gcctttggcg tcgctacggc
     4261 aacggtcatg ctgttggccc gagcagttgg agaggatgat tggaaaagag tcgctagttt
     4321 gagtaaacaa accttttggc tttctctgtt cctcatgttg cccctgtcct ttagtatata
     4381 tgtcttgggt gtaccattaa ctcatctcta tacgactgat tctctagcgg tggaggctag
     4441 tgttctagtg acactgtttt cactacttgg aacccctatg acgacaggaa cagtcatcta
     4501 tacggcagtc tggcagggat taggaaatgc acgcctccct ttttatgcga caagtatagg
     4561 catgtggtgt atccgcattg gaacaggata tctgctgggg attgtgcttg gttggggctt
     4621 gcctggtatt tgggcagggt ctcttttgga taatggtttt cgctggttat ttctacgcta
     4681 tcgttaccag cgctatatga gcttgaaagg ataggaaatg caaaaaacag cttttatttg
     4741 ggatttagac gggactttat tggactctta cgaagcgatt ttatcaggga ttgaggagac
     4801 ttttgctcag ttttctattc cttatgataa ggagaaggtg agagagttta tcttcaagta
     4861 ttcggtgcaa gatttgcttg tgcgggtggc agaagataga aatctggatg ttgaggtgct
     4921 aaatcaggtg cgtgcccaga gtctggctga gaagaatgct caggtagttt tgatgccagg
     4981 tgcgcgtgag gtgctagctt gggcagacga atcaggaatt cagcagttta tatatactca
     5041 taaggggaac aacgctttta ccattctcaa ggacttgggg gtggaatcct attttacagc
     5101 gattttaacc agtcagagtg gctttgtgcg gaagccaagt ccagaagcgg ctacctatct
     5161 gctagataag tatcagttga attctgataa tacttattat ataggggatc ggactctgga
     5221 tgtggaattt gcccagaata gtgggattca aagtatcaac tttttagagt ctacttatga
     5281 agggaatcac aggattcaag cgttagcaga tatttcccgt atttttgaga ctaagtgata
     5341 aaaagattgt gtcagttttg tgacagagac ctaacaaact atttcaagta acctagtttg
     5401 ttacaaggaa tagacagttc tgttaaatag gcccgagagg gctttttttc tacatttttt
     5461 gtgttatcat agacaggtac tcatttgaaa ggaatttgaa agaatgaaga aaagaatgtt
     5521 attagcgtca acagtagcct tgtcatttgc cccagtattg gcaactcaag cagaagaagt
     5581 tctttggact gcacgtagtg ttgagcaaat ccaaaacgat ttgactaaaa cggacaacaa
     5641 aacaagttat accgtacagt atggtgatac tttgagcacc attgcagaag ccttgggtgt
     5701 agatgtcaca gtgcttgcga atctgaacaa aatcactaat atggacttga ttttcccaga
     5761 aactgttttg acaacgactg tcaatgaagc agaagaagta acagaagttg aaatccaaac
     5821 acctcaagca gactctagtg aagaagtgac aactgcgaca gcagatttga ccactaatca
     5881 agtgaccgtt gatgatcaaa ctgttcaggt tgcagacctt tctcaaccaa ttgcagaagc
     5941 tccaaaagaa gtagcatcaa gttcagaagt tacaaagaca gtgattgctt ctgaagaagt
     6001 ggcaccatct acgggcactt ctgtcccaga ggagcaaacg gccgaaacaa gcagtgcagt
     6061 tgcagaagaa gctcctcagg aaacgactcc agctgagaag caggaaacac aagcaagccc
     6121 tcaagctgca tcagcagtgg aagcaactac aacaagttca gaagcaaaag aagtagcatc
     6181 atccaatgga gctacagcag cagtttctac ttatcaacca gaagagacga aagtaatttc
     6241 aacaacttac gaggctccag ctgcgcccga ttatgctgga cttgcagtag caaaatctga
     6301 aaatgcaggt cttcaaccac aaacagctgc ctttaaagaa gaaattgcta acttgtttgg
     6361 cattacatcc tttagtggtt atcgtccagg agacagtgga gatcacggaa aaggtttggc
     6421 tatcgacttt atggtaccag aacgttcaga attaggggat aagattgcgg aatatgctat
     6481 tcaaaatatg gccagccgtg gcattagtta catcatctgg aaacaacgtt tctatgctcc
     6541 attcgatagc aaatatgggc cagctaacac ttggaaccca atgccagacc gtggtagtgt
     6601 gacagaaaat cactatgatc acgttcacgt ttcaatgaat ggataaaccc gacttgataa
     6661 catcattttg acgaatgaga tctagctttc gtgatggaaa gcgattctcg ttcgtttttt
     6721 ctttgtcata ctcttcgaaa atctcttcaa accacgtcag ttttatctga aacctcaaag
     6781 ctgtgctttg agcaacctgc gactagcttc ctagtttgct ttttgatttt cattgagtat
     6841 caatttgaat ggaaaatgga aagttatcat cttgtaatga gttaagcaac attcttgcaa
     6901 tctattttac tttatatcac aattaattag tcaaatattg ataaatcaat aaaaagagag
     6961 gggaagaaat gctagagatt caagatttac tgtatcaact ccgcttgtct gagcaagcga
     7021 gtacgcaatt gtttgaaaaa aggcttggga ttagtttgac acggtatcag attttactgt
     7081 ttttgctgga gcattctcct tgtaaccaaa tggcgattca ggagcgtttg aaaattgatc
     7141 aggctgcttt gacacggcat ttcaagattt tggaaacgga aggtttggtg gagcgtcatc
     7201 gtaatcctga aaatcagcgg gaagtgttgg tagaggctgc gaagtatgcc aaggagcagt
     7261 tagtggtgaa tccccctctg caacatatca aggttaagga agagatggaa agtatcttaa
     7321 cagagtttga gagaacagaa ctcagccgtt tattaaataa attggttttg ggtattgaaa
     7381 atatagaaat ttaaggagaa atagatgtca attattacaa ttattttaac aacgatcgtt
     7441 gctttggagc atttttacat tttttatttg gaaagtattg ctacgcgatc agatgcgact
     7501 agtcgtgtat ttaatatgga aaaggaagaa ttggctcatc cgtcagtaag ttcattgttc
     7561 aaaaatcaag ggatttataa ggctctgcta ggagtctttc tctggtatgg catttatttc
     7621 tcacagaatt tagaaattgt gactattttt gtcttatttg tgattggtgc tgcgacttac
     7681 ggctctttaa cagcggataa aaaaattatt ttgaagcaag gtggatcagc tattttggcc
     7741 ttgattagta ttttactctt taaaatacac ttgaaggtcg attctaatct cgctaatcct
     7801 ttttaatcca gaataaggga aatatgttat acttgttttt aagaaaaaag tctcattgaa
     7861 ttggttttga ggagttagaa atgaaagtat tagtgacagg ttttgagccc tttggagggg
     7921 aaaagggcaa tccaactttg gaggccatta aaggtttacc agctgaaatc catggtgctg
     7981 aggtccgttg gctagaggtg ccgacagttt ttcacaaatc tgctcaagta ttggaagaag
     8041 agatgaatcg ttatcaacct gactttgtcc tttgtattgg gcaagctggt ggaagaacta
     8101 gtgtgacacc tgaacgagtg gccattaatc aagacgatgc acgcatttct gataacgaag
     8161 ataatcaacc gattgaccgt cccattcgcc cagatggtgc ttcggcctac tttagtagtt
     8221 tgccgattaa agcgatggtt caagctataa aaaaagaggg cttaccggcc tctgtttcca
     8281 atacggcagg gacttttgtc tgcagccatt tgatgtatca ggctctctat ttggtagaaa
     8341 agaaatttcc atatgttaag gcaggtttta tgcatattcc ttatatgatg gaacaggtgg
     8401 tgaacagacc gactactcca actatgagtt tagtggatat tcggcgaggg atagaagcag
     8461 caatcggcgc tatgatagaa catggagatc aggaactcaa gttggtaggc ggagaaactc
     8521 attgatagaa aaaagcttga ggggaaaaac cttcaagctt ttggacgttt tcgagccaat
     8581 actgctcggt aaaacataat tttagtgcat tggatataag gtaggagtga aaaactagca
     8641 atgccaaagg taatccaatt gaggaagtac caaggaagaa gctgtaaatc taggacaaag
     8701 tgctggaact tgtagccctt cataaaggaa cggctagttt ttaggattcg tcttggtggg
     8761 acctgtccta ggtctagact ataacagaga agaaattcca cctgtgaata ggcataatac
     8821 tgtggaatat agaggatatt tcctacaatg atcaagatga gacttgcaag aaagtagagt
     8881 ccaaagacca tgaggaaacg ctcggtttca actgatgaga gatctagatt tggaaactca
     8941 ggatgtaggg tgacgaattt tttggctaaa aagctactat aaaagaggag ataaatccca
     9001 agtaaattag ggatactcca taaaaagaga tagaaacgtt tgagaagtag ggtcaaaaag
     9061 gtttgagaaa agcgctcctc atcaaagaga gctaggctgt tttttacaga tggctccgtt
     9121 ttagaatctt tcatgagtgt cagtgttgca tagacggaac tggtcaaaag aatagtcccg
     9181 ataaaggaga ctagtagagg aaagaggtag gcttgaagta tttggccaag tatgctgaaa
     9241 aatggctgtt ctaaaacagt cccgtggatc cgagataagg gattaagaaa actagataag
     9301 atgaccagca tactgggaag gatatagagg agaaagagac ggggggtgtc agcctgaaaa
     9361 tgttttgact cctgacgaat tgtttttaaa tcaatttttg gatagttcat tctcttatta
     9421 taccatagtt cttatacata gttcgtgaca gttcctactt tttttgataa aatcatacag
     9481 tgtgtccttg ggcacactgt atgaactggg actgtctttc ccagcttcgg aggtaaaaaa
     9541 tgtcagattc accaatcaaa tatcgtttga ttaagaaaga aaaacacaca ggagctcgtc
     9601 taggagaaat catcactccc cacggtacct ttccgacacc tatgtttatg ccagttggga
     9661 cacaagccac tgtcaaaact cagtcacctg aagaattgaa ggagatgggt tcgggaatta
     9721 tcctatcaaa cacttatcat ctctggcttc gccctggaga tgaactcatt gcacgcgctg
     9781 gtggtctcca caagttcatg aattgggacc agcctatctt gacagatagt ggtggttttc
     9841 aggtttattc tttagcagat agccgtaata tcacagaaga aggagtaacc tttaaaaatc
     9901 atctaaatgg ttctaagatg ttcctatccc cagaaaaagc catctctatt cagaataatc
     9961 tgggttcaga catcatgatg tcctttgatg aatgtcctca gttttatcaa ccttatgact
    10021 acgttaagaa atcgatagag cgtaccagcc gttgggctga gcgtggtttg aaggctcacc
    10081 gtcgtccaca tgaccaaggt ttgtttggaa ttgtgcaagg tgcaggattt gaagaccttc
    10141 gccgccaatc agctcatgat cttgtcagca tggatttctc aggctactct atcggtggtt
    10201 tggcagtggg agaaacccat gaagagatga atgcggtctt ggactttaca actcaactgc
    10261 tgcctgaaaa taaacctcgt tatctgatgg gtgtgggagc gccagatagc ttgatcgatg
    10321 gggtcattcg tggggtggat atgtttgact gtgtcttacc gactcgaatt gctcgtaacg
    10381 ggacttgtat gaccagtcaa ggacgtttgg ttgtgaaaaa tgcccagttt gctgaggact
    10441 ttacgccact ggatcctgag tgtgattgct acacatgtaa taactataca cgcgcttacc
    10501 ttcgtcacct gctcaaggct gatgaaacct ttgggatccg cttgactagc taccacaatc
    10561 tttacttctt gcttaacctg atgaagcaag tgcgacaagc catcatggat gacaatctct
    10621 tggaattccg tgagtatttt gtggaaaaat atggctataa taagtcagga cgtaatttct
    10681 aaaatggaat tgatataagc taaaaatcct aagttttctc ttaggatttt tcttcttttt
    10741 ttgatagaat aaagtgtaca atgaaaggaa gaataaactc gtatgcgcat taaatggttt
    10801 tccttggtta ggattacagg tttacttttg gtactcttgt atcacttctt tcagacgatc
    10861 tttcctggag gatttttcgg ggtagatgtc tttttcacat tttcaggttt cctgattaca
    10921 gctctactca ttgaagaatt ttctaaaaac catgagattg atttgatagg attttttaga
    10981 agacgctttt atcggattgt gccacctgtg gttttgatgg tcttggtgac catgcctttt
    11041 actttcttgg ttcgccaaga ctatgttgct ggaattggtg gccagattgc gagcgtctta
    11101 ggctttatga ccaacttcta tgaactccta acaggtggga gttatgaatc tcagttcatt
    11161 cctcatttgt ttgttcataa ttggagtttg gcagttgagg ttcactacta tattctttgg
    11221 ggattggcag tttggttctt atccaaacaa gctaaatcaa atggtcagtt gaaggggatg
    11281 gtctttctct tatctgctgt tgccttcttg atcagtttct tctccatgtt tattggtagt
    11341 tttctagtga cctcttattc ctctgtttat ttctccagtt taactcatgt ctatccattc
    11401 tttttgggaa gtatgttagc aactattgta ggcgttcgtc agacgacttc cctcgtcaag
    11461 cagttggata aaatctggga tttacgaaag actttggtag tttttggagg aggctttggt
    11521 ttcctagttc ttttgacttt ctttgtcaaa ttcacttatc tttttgccta tcttatcggc
    11581 ttcttacttg ccagtcttgc agctcttgcc atgattctgg cggcgcgtgt cttacatgaa
    11641 aagacacatc atatacagga gccgaagatt atcagctttt tagcggatac tagctatgcg
    11701 gtttatcttt tccattggcc tttctatatc attttctcac agttgacatc aaatcttctt
    11761 gctgtattac tgactctgat ttgttcttat ggctttgcca gtctgtcatt ttatgtattg
    11821 gaaccttgga ttgcaggcaa gaacacacct attgtccaaa cccttcgtcc cctgccttat
    11881 attcacgcaa ttcttgcagc aggtacagga atcttgacca tcattgtctg cacggtgacc
    11941 ttgttggcac cacaagtggg agcgtttgag acagacttga ctgtcaatgg cttgaagcaa
    12001 gctgcaacaa atattggcca gaccaaggtg atggcagaac gggcagatgc aaacagtttg
    12061 ggaattgctg atggcactat gttaattggt gactcagtgg ctttaagggc aaatacagca
    12121 ctacagacag ctcttcctgg agcacagatt aacgcgcagg tcagcgtaac aaccaagacc
    12181 gcaaatgaaa tcatgctaaa taatagccag aataaatttt tacctaagac ggtggtcatt
    12241 gcgactgggg taaataatcc tgagaattac aaggatgact gggacagtat cgtgaaaaat
    12301 cttcctaagg gacaccatat gattttggtg actccttatg agggagataa gacaaaagag
    12361 acctatgcca tcgttgagaa ggctgctgcc tatatgagag aattggcaga gaagacacct
    12421 tacattacga tagcagattg gaatcaagtt gcgaaagagc atccagaaat ttgggctgga
    12481 acagaccagg ttcatttcgg gagtgagagt agcactatcg aagcaggagc aaaattgtat
    12541 gcagatacga ttgccacagc tttgcagaca gctcaagaca agccggttaa atcaaaataa
    12601 cttgtattaa aaaagagaag agttggagaa atccagctct tttaaaatat ctccaaaaaa
    12661 taggtctata ccatttacaa ataaaaaaga aaggtttata atgtaattga cataataaat
    12721 tgtagaatca atcttttaag gaggttaaca ttatgcctaa ctatattaaa gcggatcagt
    12781 ttttctaccc acacggagtt cgtcgaggtg gttacttgga acttgtggac ggcaagtttg
    12841 ggaaacatgt agagcagatt cctgaagggg ctgaggtgat tgactataca ggttatagca
    12901 ttgccccagg tcttgtggat actcatattc atggatatgc aggtgtagat gtgatggaca
    12961 acaacattga aggtacattg catactatga gtgaaggact tcttagtacc ggtgttacca
    13021 gtttcttacc cacaacttta acagccactt atgagcaatt gcttgcagtc actgaaaatc
    13081 ttggaaacca ttataaagaa gcaacaggtg ctaagattcg tgggatttat tatgaaggtc
    13141 catatttcac agaaactttt aagggggcac aaaatccaac ttatatgaga gacccgggtg
    13201 ttgaggagtt tcattcttgg caaaaagcgg caaatggctt gcttaataaa attgcccttg
    13261 caccagaacg tgatggggtg gaagactttg ttcgtacagt tacgggcgaa ggtgtgacgg
    13321 ttgctcttgg acattcaaac gcgacttttg atgaagccaa aaaagcagtc gatgctggag
    13381 cgagtgtttg ggtgcatgcc tacaatggaa tgcgtgggtt gactcaccgt gaattgggta
    13441 tggttggagc catgtaccaa ttgccacata cctatgcaga gttgatctgt gatggtcacc
    13501 acgtagatcc aaaggcttgc gaaattctta tcaaacaaaa aggaacagaa aacatcgctc
    13561 ttatcacaga ctgtatgaca gctgggggat tggaagacgg agattatatg ttgggagaat
    13621 tcccagttgt cgttgcaaat ggaactgcac gcctcaaatc gacaggtaac ttggcaggtt
    13681 ctatcctcaa actcaaagat ggtttgaaga atgtggtcga atggggaatt gcgaatccgc
    13741 atgaagcagt catgatggcc agcttcaacc cagctaaatc cgttcacatc gatgacgtct
    13801 gtggccaaat ccgtgaaggc tacgacgctg acttcatcgt attagataaa gatttggaat
    13861 tggtagcaac ctacctagat ggcgtaaaac gttatcaagc ataagagaga aagcagaagt
    13921 tagagactaa cttctgcttt tttatgcagg gtactttact ggtaaataaa aagttaaaaa
    13981 aatcacaaaa aaagcttgaa gaaacaaaga aaaaggctta caattgaact ataaatatta
    14041 caaataaaaa aacggaggag tgctttatga aagcctatac ttatgttaaa ccaggacttg
    14101 cttcttttgt tgatgtagac aaaccagtta ttcgcaagcc aacagatgct attgtgcgta
    14161 ttgtaaaaac cactatttgt ggaacagacc tccatattat caaaggggat gttcctactt
    14221 gccaaagtgg taccattctt ggccacgaag ggattgggat tgttgaagaa gttggggaag
    14281 gagtttctaa cttcaaaaaa ggtgacaagg tcttgatttc ttgcgtctgt gcctgtggta
    14341 aatgctacta ctgtaaaaaa ggaatttatg ctcactgtga agacgaaggg ggctggattt
    14401 ttgggcattt gattaatggt atgcaggctg aatacctacg tgtacctcat gcagataata
    14461 ctctttacca tacgccagca gacttgtcag acgaagcttt ggttatgcta tcagacattc
    14521 tgcctactgg atatgaaatt ggtgtcttaa aagggaaagt agaacctggt tgcagcgtag
    14581 ccattattgg ttcaggtcca gttggattgg ctgctctttt aacagcccaa ttctattcac
    14641 cagctaaatt gattatggta gacctagacg ataaccgctt ggaaactgca gtatcctttg
    14701 gtgctactca taaggtaaac tcttcagacc ctgaaaaagc cattaaagaa atttatgatt
    14761 tgacagatgg tcgtggtgtg gatgtcgcta tcgaagctgt tggtattcct gcaacatttg
    14821 atttctgtca aaagattatc ggtgtagacg gaacggttgc caactgtggt gtgcatggta
    14881 aaccagttga attcgattta gataaacttt ggattcgcaa catcaatgta acaactggtt
    14941 tggtatctac aaatacgact ccacaattgt tgaaagcact tgaaagtcat aagattgaac
    15001 cagaaaaatt ggtaactcac tatttcaaac ttagtgaaat tgaaaaagcc tacgaagtct
    15061 tcagtaaggc agcagaccac catgccatta aggtcattat cgaaaacgat atctcagaag
    15121 cctaagtagt aaaaatattt ttgtacataa gcaaatagaa attcagtcat ccatcagatg
    15181 gctggatttt ttatcaaaaa attaagaaat gagcatattt ctttccttgt ctggcggaat
    15241 tggttataat atacggtaca aaggaatgaa tgaatatgta tcgtgttata gaaatgtacg
    15301 gagattttga accgtggtgg ttcttagaag gttgggaaga agatattgta gcaagtagaa
    15361 aatttgacca gtattatgat gctctcaaat actacaaaac ttgctggttt agattggaac
    15421 aagaatcgcc tctttataaa agtagaagcg acttgatgac cattttttgg gacccggaag
    15481 accaacgctg gtgtgatgaa tgtgatgagt atttacaaca ataccattct ttggctcttt
    15541 tgcaggatga gcaggttatc ccagacgaaa aactacgctc aggctatgaa aaacaaacca
    15601 gtcaggaaag gaatcgttct tgccgtatga aattaaaata gagaaaagta acttttttgg
    15661 agttgctttt tttatttttc taactctttg cgaatagtat aggtgaggag gtaagtatgg
    15721 ttcaagaaat tgcacaagaa atcattcgtt cagctcggaa aaaagggacg caggatatct
    15781 attttgtccc taagttagac gcctatgagc ttcatatgag ggtaggagac gagcgctgta
    15841 aaattggtag ctatgatttt gaaaagtttg cagccgttat cagtcacttt aagtttgtgg
    15901 cgggtatgaa tgtgggagaa aaaagacgta gtcaactggg ttcctgtgat tatgcctatg
    15961 accagaagat agcgtctcta cgtttatcta ctgtaggcga ttatcggggg catgagagtt
    16021 tggttatccg tttgttgcac gatgaggagc aggacctgca tttttggttt caggatattg
    16081 aagaattagg caagcagtac aggcaacggg gactctatct ttttgctggt ccggttggga
    16141 gtggtaagac gaccttgatg catgaattgt ccaagtcact ctttaaagga cagcaagtta
    16201 tgtccatcga agatcctgtc gaaatcaagc aggacgacat gcttcagttg cagttgaacg
    16261 aagcaatcgg cctaacctat gaaaatctaa tcaaactttc cttgcgtcat cgaccagatc
    16321 tcttgattat cggagaaatt cgtgacagcg agacggcgcg tgcagtggtc agagctagtt
    16381 tgacaggtgc gacagtcttt tcaaccattc acgccaagag tatccgaggt gtttatgagc
    16441 gtctgctgga gttgggtgtg agtgaagaag aattggcagt tgttttgcaa ggagtctgct
    16501 accagagatt aatcggggga ggaggaatcg ttgactttgc aaacagagat tatcaagaac
    16561 accaagcagc caagtggaat gagcaaattg accagcttct taaagatgga catatcacaa
    16621 gtcttcaggc tgagacggaa aaaattagct acagctaagc aaaaaaatat catcacccta
    16681 tttaacaatc tcttttctag cggttttcat ctggtggaga ctatctcttt tttagatagg
    16741 agtgctttgt tggacaagca gtgtgtgacc cagatgcgtg tgggcttgtc tcaggggaaa
    16801 tcattctcag aaatgatgga aagtttggga tgttcaagtg ctattgtcac tcagttatcc
    16861 ctagctgaag ttcatggcaa tctccacctg agtttgggaa agatagaaga atatctggac
    16921 aatctggcca aggtcaagaa aaaattgatt gaagtagcga cctatccctt gattttgctg
    16981 ggttttcttc tcttaattat gctggggcta cggaattacc tgctcccaca actggatagt
    17041 agcaatattg ccacccaaat tatcggtaat ctgccacaaa tctttctagg aatgatagtt
    17101 tttgtttcag taggtgtcct tttagcactc actttttata aaagaagttc taagatgcgc
    17161 gtcttttcta tcttagcgcg ccttcctttt attggaatct ttgtgcagac ctatttgaca
    17221 gcctattatg cacgtgaatg ggggaatatg atttcacagg gaatggagtt gacgcagatt
    17281 tttcaaatga tgcaggaaca aggttcccag ctctttaaag aaatcggtca agatctggct
    17341 caaaccctga aaaatggccg tgaattttct cagacgatag gaacctatcc tttctttagg
    17401 aaggaattga gtctcatcat agagtatggg gaagttaagt ccaagctggg tagtgagttg
    17461 gaaatctatg ctgaaaaaac ttgggaagcc ttttttaccc gagtcaaccg caccatgaat
    17521 ttggtgcagc cactggtttt tatctttgtg gcactgatta tcgttttact ttatgcggca
    17581 atgctcaggc ccatgtatca aaatatggag gtaaattttt aaaatgaaaa aaatgatgac
    17641 attcttgaaa aaagctaagg ttaaagcttt tacattggtg gaaatgttgg tggtcttgct
    17701 gattatcagc gtgcttttct tgctctttgt acctaatctg accaagcaaa aagaagcagt
    17761 caatgacaaa ggaaaagcag ctgttgttaa ggtggtggaa agccaggcag aactttatag
    17821 cttggaaaag aatgaagatg ctagcctaag caagttaaaa gacgatgggc gaatcacgga
    17881 agaacaggtt aaagcttata aagaatacaa tgataaaaat ggaggagcaa atcgtaaagt
    17941 caatgattaa ggcctttacc atgctggaaa gtctcttggt tttgggtctt gtgagtatcc
    18001 ttgccttggg cttgtccggc tctgttcagt ccacttttgc ggcagtagag gaacagattt
    18061 tctttatgga atttgaagaa ctctatcggg aaacccaaaa acgcagtgta gccagtcagc
    18121 aaaagactag tctgaactta gatgggcaga cgattagcaa tggcagtcaa aagttgccag
    18181 tccctaaagg aattcaggtc ccatcaggcc aaagtattac atttgaccgt gctgggggca
    18241 attcgtccct ggctaaggtt gaatttcaga ccagtaaagg agcgattcgc tatcaattat
    18301 atctaggaaa tggaaaaatt aaacgcatta aggaaacaaa aaattagggc agtgatttta
    18361 ctggaagcag tagtcgctct agctatcttt gccagcattg cgaccctcct tttgggacaa
    18421 attcaaaaaa ataggcaaga ggaagcaaaa atcttgcaaa aggaagaagt cttgagggta
    18481 gctaagatgg ccttgcagac aggtcaaaat caggtaaaca taaacggagt tgagattcag
    18541 gtgttttcta gcgaaaaggg attggaggtc taccatggtt cagaacagtt gttggcaatc
    18601 aaagagccat aaggtcaagg cttttacctt gttagaatcc ctgcttgccc tgcttgtcat
    18661 cagtgggggc ttactccttt ttcaagctat gagtcagctc ctcatttcag aagttcgcta
    18721 ccagcaacaa agcgagcaaa aggagtggat tttgtttgtg gaccaacttg aggcagaatt
    18781 agaccgttcg cagttcgaaa aagtagaagg caatcgccta tacatgaagc aagatggcaa
    18841 ggacatcgcc atcggtaagt caaagtcaga cgattttcgt aaaaccgatg ctagtggacg
    18901 ggggtatcag cctatggttt atggcctcaa atcagctcag attacagagg acaatcaact
    18961 ggttcatttt cgtttccagt ttcaaaaagg cttagaaagg gagttcatct atcgtgtgga
    19021 aaaagaagaa agttaaggca ggtgttctcc tctacgcagt caccatagca gccatcttta
    19081 gtcttttgtt gcaattttat ttgaaccgac aaatcgccca ctatcaagac tatgctttaa
    19141 ataaagaaaa attggttgct tttgccatgg ctaaacgaac caaggataaa gccgagcaag
    19201 aaagtgggga acaggttttt aatctaggtc aggtaagcta tcaaaacaag aaaacaagct
    19261 tggtgacgac tgttcgtacg tctaagagcc aatatgagtt tctgtttcct tcagtcaaaa
    19321 tcaaagaaga gaaaagagat aaaaaggaag aggtagcgac cgattcaagc gaaaaagcgg
    19381 agaaaaaaaa tcagaagaga agcctgaaaa gaaagagaat tcctagtcaa ttcaactata
    19441 atgcgttgaa tccagaatag tccactgtag tttctagaaa attgctggaa atggatgtta
    19501 agctccaatt catttgttta tatcttattt cagtttacta tactttatgc taaattaaag
    19561 atatgaaaca tgattttaac cacaaagcag aaactttcga ttcccctaaa aatatcttcc
    19621 tcgcaaactt ggtatgtcaa gcagtcgaga aacagattga tattttatca gacaaagaaa
    19681 ttttagattt cggtggtggc acgggtctat tagtcttgcc cctagccaag caggctaagt
    19741 cagtcactct tgtagacatt tctgagaaaa tgttggagca agctcgtttg aaagtggagc
    19801 agcaagcaat caagaatatc cagtttttgg agcaagattt accgaaaaat cccttggaga
    19861 aagagtttga ttgccttgct gttagtcggg ttcttcatca tatgcctgat ttggatgcgg
    19921 ctctctcact gtttcatcaa catttgaagg aagatgggaa actcatcatt gctgatttta
    19981 ccaagacaga agctaatcat catggatttg atttagctga actggaaaac aagctaattg
    20041 agcatggttt ttcatctgtg catagtcaga ttctctatag cgctgaagac ctgtttcaag
    20101 gaaatcactc agaattcttt ttaacagtag cccaaaaatc actcgcctag tcagggagtg
    20161 atttttctat aaggatggaa aaaagaaggg aaatttggta agataggaat atggattttg
    20221 aaaaaattga acaagcctat acctatttac tagagaatgt ccaagtcatc caaagtgatt
    20281 tggcgaccaa cttttatgac gccttggtgg agcaaaacag catctatctg gatggtgaaa
    20341 ctgagctaaa ccaggtcaaa gacaacaatc aggcccttaa gcgtttagca ctacgcaaag
    20401 aagaatggct caagacctac cagtttctct tgatgaaggc tgggcaaaca gaacccttgc
    20461 aggccaatca ccagtttaca ccagatgcca ttgctttgct tttggtgttt attgtggaag
    20521 agttgtttaa agaggaggaa attactatcc tcgaaatggg gtctgggatg ggaattctag
    20581 gcgctacttt cttgacctcg cttgataaaa aggtggatta cttgggaatg gaaatggatg
    20641 atttgctgat tgatctggca gctagcatgg cagatgtaat tggtttgcag gctggctttg
    20701 tccaaggaga tgccgttcgt ccacaaatgc tcaaagaaag cgatgtggtc atcagtgact
    20761 tgcctgttgg ctattatcct gatgatgccg ttgcgtcgcg ccatcaagtt gcttctagcc
    20821 aagaacatac ttacgcccat cacttgctca tggaacaagg gcttaagtac ctcaagtcag
    20881 acggatacgc tatttttcta gctccgagtg atttgttgac cagtcctcaa agtgatttgt
    20941 taaaagaatg gctgaaagaa gaggcgagtc tggttgctat gattagtctg cctgaaaatc
    21001 tctttgctaa taccaaacaa tctaagacta tttttatctt acagaagaaa aatgaaatag
    21061 cagtagatcc ttttgtttat ccacttgcta gcttgcaaga tgcaagtgtt ttaatgaaat
    21121 ttaaagaaaa ttttcaaaaa tggactcaag gtactgaaat ataaaataga ttttgttata
    21181 atagttgaaa acgcttaaaa aggggtatca tgttatgaca aaaacaattg caatcaatgc
    21241 aggaagttca agtttgaaat ggcaattata cttaatgcca gaagaaaaag tattggcgaa
    21301 aggtttgatt gaacgtatcg gtttgaaaga ttcaatttca actgtaaaat ttgacggccg
    21361 ttctgagcaa caaattttgg atattgaaaa tcatacacaa gccgttaaaa ttttattgga
    21421 tgacttgatt cgtttcgata ttatcaaggc ttatgacgag attacaggtg ttggacatcg
    21481 tgttgttgct ggtggagaat atttcaaaga atcaacagtt gttgagggag atgttttaga
    21541 aaaagttgaa gagttgagtt tgttggctcc tctacacaac ccggccaatg cagcaggtgt
    21601 tcgtgccttc aaggaattgt tgccagacat taccagtgta gttgtttttg atacttcctt
    21661 ccacacaagt atgccagaga aagcttatcg ctaccctcta ccaacaaaat attacacaga
    21721 aaacaaggtt cgtaaatacg gtgctcatgg tacaagtcac cagtttgtag caggagaggc
    21781 tgcaaaactc ttgggacgtc cattagaaga cttgaagtta attacctgtc atattggtaa
    21841 cggaggctca attacagctg tgaaagccgg caaatctgta gacacttcta tggggttcac
    21901 tcctcttggt ggtattatga tgggaacgcg tacaggggat attgatccag ctatcattcc
    21961 ttatttaatg caatatacag aggattttaa cacaccagaa gatatcagtc gtgttcttaa
    22021 ccgtgaatca ggtcttttgg gagtttctgc taattctagc gatatgcgcg atatagaagc
    22081 agctgtagca gaagggaatc acgaggctag cttggcttat gaaatgtatg ttgaccgtat
    22141 ccaaaaatat atcggtcagt accttgcagt gctaaatgga gcagatgcca ttgttttcac
    22201 agcaggtgtc ggtgaaaatg cagagaattt ccgtcgtgat gtaatctcag ggatttcgtg
    22261 gtttggttgt gatgttgatg atgaaaagaa tgtctttggc gttacaggag acatctcaac
    22321 agaggcagct aaaatccgtg tcttggttat tccaacagat gaagaattag tcattgcccg
    22381 tgacgttgaa cgcttgaaaa aataagtgaa actaaaaaaa tattcaatac aaggagttgg
    22441 gaaagttatt tttccagctt ctttttctga tgaaattgtc caaaaccttg ctatgattgg
    22501 cttttttgaa aaatatggta taatagtagt aatttaatag atggagttga gttttgaaga
    22561 aaaactttcg tgtaaaaaga gagaaagatt ttaaggcgat tttcaaggag gggacaagtt
    22621 ttgctaatcg caaatttgtg gtctaccaat tagaaaacca gaaaaaccat tttcgagtag
    22681 gtctatcagt tagcaaaaaa ctggggaatg ccgtcactag aaatcaaatt aagcgacgga
    22741 ttcggcatat tatccagaat gcaaaaggga gtctggtaga agatgtcgac tttgttgtca
    22801 ttgctcgaaa aggagtcgaa accttgggat acgcagagat ggagaaaaat ctactccatg
    22861 tattaaaatt atcaaagatt taccgggaag gaaatgggag tgaaaaagaa actaaagttg
    22921 actagtttgc taggactgtc tctgttaatc atgacagcct gtgcgactaa tggggtaact
    22981 agcgatatta cagccgaatc ggctgatttt tggagtaaat tggtttactt ctttgcggaa
    23041 atcattcgct ttttatcgtt tgatattagt atcggagtgg ggattattct ctttacggtc
    23101 ttgattcgta cagtcctctt gccagtcttt caggtgcaaa tggtggcttc taggaaaatg
    23161 caggaagctc agccacgcat taaggcgctt cgagaacaat attcaggtcg agatatggaa
    23221 agcagaacca aactagagca ggaaatgcgt aaagtattta aagaaatggg tgtcagacag
    23281 tcagactctc tttggccgat tttgattcag atgccggtta ttttggccct gttccaagcc
    23341 ctatcaagag ttgacttttt aaagacaggt catttcttat ggattaacct tggtagtgtg
    23401 gatacaaccc ttgttcttcc gattttagca gcagtattca cctttttaag tacttggttg
    23461 tccaacaaag ctttgtctga gcgaaatggc gctacgactg cgatgatgta tgggattcca
    23521 gtcttgattt ttatctttgc agtttatgcg ccaggtggag tcgccctata ctggacagtg
    23581 tctaatgctt atcaagtctt gcaaacctat ttcttgaata atccattcaa gattatcgca
    23641 gagcgcgagg ccgtagtaca ggcacaaaaa gatttggaaa atagaaaaag aaaagccaag
    23701 aaaaaggctc agaaaacgaa ataaataagg aggaatctgg taatggtagt atttacaggt
    23761 tcaactgttg aagaagcaat ccagaaagga ttgaaagaat tagatattcc aagaatgaag
    23821 gctcatatca aagtcatttc tagggagaaa aaaggctttc ttggtctatt tggtaaaaaa
    23881 ccagcccaag tggatattga agcgattagt gaaacgactg ttgtcaaagc aaatcaacag
    23941 gtagtaaaag gcgttccgaa aaaaatcaat gatttgaacg agcctgtgaa gacggttagt
    24001 gaagaaaccg ttgaccttgg tcatgtggtt gatgctatta aaaaaataga ggaagaaggt
    24061 caaggtattt ctgatgaagt caaggctgaa atcttaaaac atgaaagata tgccagcact
    24121 atcttagaag aaactggtca cattgagatt ttaaatgaac ttcaaatcga ggaagcgatg
    24181 agggaagaag caggcgctga tgaccttgaa actgagcaag atcaaactga aaatcaagac
    24241 ttgaaagaga tgggcttgaa ggtcgagcaa agttatgata ttgcccaggt ggctacggat
    24301 gtgactgctt atgttcaaac gattattgat gacatggatg ttgaggctac actttcaaat
    24361 gattataacc gtcgtagcat caatttgcaa attgatacta acgaaccagg tcgtattatc
    24421 ggctaccatg gtaaagtctt gaaggccttg caactgttgg ctcaaaatta tctttacaac
    24481 tgctattcaa gaaccttcta tatcactatt aatgtcaatg attatgttga acaccgtgca
    24541 gaagtcttgc agacctatgc gcaaaaattg gcgattcgtg ttttagaaga aggacgtagt
    24601 catcaaacag atccaatgtc gaatagtgaa cgcaagatta tccaccgtat tatttcacgt
    24661 atggatggcg tgaccagtta ctctgaaggt gatgagccaa atcgctatgt cgttgtagat
    24721 acagaataag taaaatcagg gttgacctga ttttttgcta gctagaggag gttaaactga
    24781 tgttgaataa gataagagac tatctagact tttctggttt gcagtaccgt agtcctgata
    24841 aagcgggagc agagcgagag aagatgctgg cgttccgcca caaaggacaa gaggcccgaa
    24901 aggcttttac agaactggcc aaagcctttc aagcaagcca tccagaatgg caactccaac
    24961 agactagtca gtggatgaat caggcccagc gtttgagacc acatttttgg gtttatctac
    25021 agagagacgg acaagtgaca gaacctatga tggccttacg tttgtatggg acatctactg
    25081 actttggaat ttctttggaa gtcagtttca tcgaacgtaa gaaggatgag caaacactgg
    25141 gcaagcaggc caaagtttta gacattccaa ccgttaaagg gatttattat ctaacctact
    25201 ctaatggtca aagtcaacgg tgggaggcga atgaagaaaa gcgtcgtact ttacgcgaga
    25261 aggtgagaag tcaagaagtt cgaaaagttt tagtgaaggt agatgttcct atgacagaaa
    25321 attcgtctga agaagaaatc gtagaaggct tattgaagtc ttattctaaa attcttccct
    25381 attatctagc tacgagaaaa taagataatt tgtaaaacat cataaatcat acagtccaag
    25441 agtgaacagt ccgctgtgta attcttggtc tttttgtttg cgctttcgca ttatataata
    25501 aacttacaaa aacaattcaa aaggagaaca attatggaag tcgtttcaag tgttctaaat
    25561 tggttttcta gcaatatttt gcagaatccc gcatttttcg taggtttatt ggtgttgata
    25621 ggatatgcac ttttgaaaaa acctgcccat gacgtttttt cagggtttgt taaagcaaca
    25681 gtagggtata tgttgcttaa cgtgggtgct ggtggtttgg ttacaacctt tcgtccaatc
    25741 ttagcagctc ttaactacaa attccaaatt ggtgcagcgg ttatcgaccc ttattttgga
    25801 cttgctgcag caaacaacaa aattgtagca gagtttccag attttgttgg aactgcaact
    25861 acagctctat tgattggttt tggaataaat atcttgctcg tagctcttcg aaagattacg
    25921 aaggtaagaa ccctctttat tactggtcac atcatggtac aacaagctgc aacagtatct
    25981 cttatggttc tattcttagt accacaattg cgcaatgctt acggtacagc agcgattggt
    26041 atcatctgtg gactttactg ggcagttagt tcaaatatga ctgttgaggc aactcaacgc
    26101 ttgactggtg gtggcggatt tgcgattggt caccaacagc aatttgcaat ctggtttgta
    26161 gataaagtag caggacgctt tggtaagaaa gaagaaagtt tagacaatct taaattacct
    26221 aagttcctct caatcttcca cgatacagtt gttgcatctg ctaccttgat gctcgtattc
    26281 ttcggagcca ttcttttaat cttgggtcca gacattatgt ctaataaaga agtcatcact
    26341 tcaggaactc tattcaatcc tgctaaacaa gatttcttta tgtacattat ccaaacagcc
    26401 tttaccttct cagtttactt gttcgttttg atgcaaggtg tccgaatgtt cgtatctgag
    26461 ttgacaaacg ccttccaagg tatttcaaac aaattgttgc caggttcatt cccagcggtt
    26521 gacgttgcag cttcttatgg atttggttct ccaaatgctg tcttgtcagg atttaccttt
    26581 ggtttgattg gtcaattgat tacaattgtt ttgctcatcg tctttaaaaa tccgattctt
    26641 attattacag gatttgtacc agtgttcttt gacaatgcag ccattgcggt ctacgctgat
    26701 aaacgcggcg gatggaaagc ggctgttatc ctttccttta tatcaggtgt ccttcaagtt
    26761 gctctaggag ctctttgtgt ggcccttctc gatttggcat cttatggtgg ctaccatgga
    26821 aatatcgact ttgaattccc atggcttgga tttggatata tcttcaaata ccttggtatt
    26881 gttggttatg tacttgtgtg tctcttcttg cttgttattc ctcaacttca atttgccaaa
    26941 gcaaaagata aagagaaata ttacaacggt gaagttcaag aagaagctta gtatctagaa
    27001 aaggagaaat aaaatggtta aagtattagc agcgtgcgga aatggaatgg gttcatcaat
    27061 ggttatcaag atgaaggttg aaaatgctct ccgtaagctt aatcaaacag attttacagt
    27121 caattcatgc agtgtcggtg aagctaaagg tttagcagta ggatatgaca ttgtaatcgc
    27181 ttctcttcat ttgattcaag aattggaagg gcgaactaat gggaagttaa ttgggcttga
    27241 taacttgatg gatgataaag aaatcaccga aaaactcagt caagcactac agtaaaaggt
    27301 tggagggggc tggacagaaa ctgagagtta tcgtttctgt ccagccccct ctttaaataa
    27361 aggaggcaga tatgaattta aaacaagctt taattgacaa tgactcgatc cgactaggtt
    27421 tagaggctaa cgattggaaa gaagcagtca aggtagcagt agatcccttg attgaaagtg
    27481 gggcaatttt gccagagtat tacgatgcta tcattgaatc gactgaagag tatgggcctt
    27541 actatatctt gatgccaggt atggctatgc cccacgctag acctgaagct ggtgtgcaaa
    27601 gtgatgcctt ttcattgatt accttacaaa atcctgttgt attttcagat gggaaagagg
    27661 tatctgtttt gttggcacta gcagcaacaa gttcaaaaat tcacacaagt gtagccattc
    27721 cacaaattat tgccctgttt gaattagaag attctattgc acgtttacag gcttgccaga
    27781 ctaaagaaga tgtcttggct atgattgaag aatctaagga tagcccttat ctcgaaggat
    27841 tggatttgga aagttagaaa gaggaataaa gaaatgacaa aaagaatacc taatttacaa
    27901 gttgcattag accattcaga cttgcaagga gcgattaaag cagctgtttc tgttggtcag
    27961 gaagtagata ttatcgaagc tggaactgtt tgcttgcttc aagttggaag tgaactggct
    28021 gaagtcttgc gtagcctttt cccagataag attattgtgg cagacacaaa atgtgctgat
    28081 gctggtggaa cagttgctaa aaataatgcg gttcgtggag cagactggat gacttgtatc
    28141 tgttgtgcaa ccatccctac tatggaagca gctctaaagg ctatcaagac tgaacgagga
    28201 gaacgaggcg aaatccagat cgagctttat ggcgattgga cttttgaaca agctcagctt
    28261 tggctagatg caggtatctc acaagctatt tatcaccaat ctcgtgatgc tcttcttgct
    28321 ggtgaaactt ggggtgaaaa agaccttaat aaggttaaaa aactcattga catgggcttc
    28381 cgtgtatctg taacaggtgg tctagatgta gatactctca aactctttga aggtgttgat
    28441 gtctttacct ttatcgcagg tcgtggaatt acagaggctg cggatccagc aggagcagcg
    28501 cgtgccttca aggatgaaat caaacgaatt tgggggtaaa tcatggtacg tccaattgga
    28561 atttatgaaa aggcaacccc aacacacttt acttggctag aacgtttaaa ttttgccaag
    28621 gagttaggct ttgattttgt cgagatgtct attgacgaac gtgacgagcg tttagcaaga
    28681 cttgactgga gtaaggaaga acgcttggaa gttgtcaaag caatctatga aactggtgtt
    28741 cgtattcctt ctatctgttt ttcaggccat cgtcgctacc cattgggttc aaaagatcca
    28801 gttctagagg aaaaatctct agaactcatg aaaaaatgta tcgaattagc tcaagacttg
    28861 ggagttcgta cgattcaatt agctggttac gatgtttact atgaggaaaa gtcaccccag
    28921 acacgccaac gttttatcaa aaatttgaga aaagcctgtg actgggctga agaagctcag
    28981 gtggtacttg ctattgaaat tatggatgat cctttcatca gtagcatcga aaaatatttg
    29041 gctatagaaa aagagattga ctctcccttc ctctttgtat atccagatat tggtaatgtg
    29101 tctgcatggc ataatgatat ctatagtgag ttttatcttg gtcatcatgc catcgcagct
    29161 ctccatctca aggatactta tgcagtgaca gaaagttcaa agggccagtt ccgagatgta
    29221 cctttcgggc aaggttgtgt taaatgggaa gaagctttcg atattttaaa ggaaaccaat
    29281 tataatggac ctttcctaat cgaaatgtgg tctgaaaatt gtgaaacagt agaagaaaca
    29341 cgcgcagccg ttcaagaggc gcaagctttt ctctatccac tcattaagaa agcaggtttg
    29401 atgtaagatg aatcaagtaa tcaatgctat gcgtaaacga gtctgtgatg ccaatcaatc
    29461 attgccaaaa catggacttg tcaaatttac ctgggggaat gtatctgaag ttaatcgcga
    29521 actcggtgtc attgttatca aaccatcagg cgtggattat gacgaattga cacctgaaaa
    29581 catggtagtg actgatctag atggtaagat cctagaaggg gatttaagac catcttccga
    29641 cctcccaact catgtgcaat tatataaggc ttggtcagaa attggtagtg tggttcacac
    29701 ccattcgaca gaagctgttg gttgggctca ggcaggtcgt gatattcctt tctacggaac
    29761 aacccatgca gattatttct acggttcaat cccttgcgcc cgtagtttga ccaaggacga
    29821 agtagaagtg gcctatgaaa aagatactgg cctggttatc gtagaagagt ttgaacatcg
    29881 cggacttaac ccggttgaag taccaggaat tgttgtacgc aatcacggtc cattcacctg
    29941 gggcaaaaat ccagagaatg ctgtttatca ctctgtcgta ctagaggaag tatcaaagat
    30001 gaatcgattt acagaacaaa tcaatccaag agttggacct gctccccagt acatactaga
    30061 aaaacactac caacgtaaac atggaccaaa tgcttattat ggtcaaaagt aagaacgatg
    30121 aaggaggaga aaaagataaa tttagctcct ctttttacat ttgattttta ttgagagtaa
    30181 agttggaatt gaagtaattt taaaagattt tttagaaata gcgcttgata tatatatatg
    30241 gtaaaataaa aagaattgct gtgatatcaa tagatttggg ggatttttta atatggtact
    30301 ggataaggca agttgtgatt tgcttcaata tttgatggat caagaaacgt ccaaaacgat
    30361 tatggcgatt tcgaaagatt tgaaagagtc aagaaggaaa atttattatc acattgacaa
    30421 aatcaatgct gctctggttg acgaggcgct tcacatcatt agtattccac gaattggtat
    30481 tcacttaacg gaagagcaga gagatgcttg ttgtaaacta ttatcggaag tagattcgta
    30541 cgattatatc atgagtgcgc atgaacgtat gatgataatg ttactatgga taggtatttc
    30601 taaagaacgt attacgattg aaaaattgat agagttaaca gaggtatcta ggaatactgt
    30661 tctcaatgat ttgaatagta ttcgttatca actaactttg gaacaatatc aggtgatctt
    30721 gcaagtgagc aagtcacagg gataccacct tcatgcccac cctcttaata aaattcagta
    30781 tcttcaatcg cttctatatc atatttttat ggaagaaaat gccacttttg tatctatttt
    30841 agaagataag atgaaagaga ggttagatga tgagtgtttg ctttctgttg aaatgaacca
    30901 attttttaag gaacaggttc ctttagttga acaagattta gggaagaaaa taaaccatca
    30961 tgaaataact tttatgttgc aggttctacc ttatttgctg ttaagctgtc ataatgttga
    31021 acagtatcaa gaaagacatc aggatataga gaaagaattt tctttgataa gaaaaagaat
    31081 agagtatcag gtgtctaaga aattaggaga acggttgttt caaaagtttg aaatttcttt
    31141 gtcaggactt gaagtttctc ttgtagctgt tctcctcctc tcctatcgta aagatttgga
    31201 tattcatgca gaaagtgatg attttcggca attaaaactt gctttagaag aatttatctg
    31261 gtattttgaa tcacaaatcc gaatggagat tgagaacaag gatgatttgt tacgaaattt
    31321 gatgatccac tgtaaagcct tgttatttag aaagacttac ggtatttttt ctaaaaatcc
    31381 tctaacaaaa caaattcgat ccaagtatgg agaattattt ttagtcacta gaaaatctgc
    31441 ggaaatttta gaaggagcat ggtttattcg gctaacagac gatgatattg cctatttgac
    31501 gattcatatt ggaggatttt taaagtatac accatcgtct caaaaaaata tgaaaaaagt
    31561 ttatctcgtt tgtgatgaag gtgttgcggt ttcgagactt ttgctgaaac aatgcaaact
    31621 ttattttcca aatgagcaaa ttgacactgt atttacaaca gaacaattta agagtgtgga
    31681 agatattgca caagttgatg tagtgattac tactaatgat gatttggata gcagatttcc
    31741 gattttaagg gttaatccta tccttgaagc agaagatatt ttgaaaatgc tagactatct
    31801 taaacacaat atatttcgta atgagagcaa aagtttcagt gaaaatcttt ctagtcttat
    31861 ttcgtcttat attgtagata gcaagttggc tagtaagttc caagaagagg ttcaaacact
    31921 tataaatcaa gaaatagtag ttcaagcttt tttggaagat atttgaagga cagtccaatg
    31981 atgaacacaa acctgtgttt ttcttggtct tttttagtgt tttgaagggt ggtatactaa
    32041 tctcaaagat aacaattata tccaaaggag gcaacatatg ccaaacgtca aagaaattac
    32101 aagagagtca tggattttag ccactttccc agagtgggga acatggttga acgaagaaat
    32161 cgaagaagaa gtcgtacctg aaggcaactt tgccatgtgg tggctaggca actgtggtac
    32221 ttggattaag acaccagctg gtgctaacgt tgtcatggac ctttggtcaa accgtggaaa
    32281 atcaaccaaa aaagtgaaag atatggttcg tgggcaccaa atggcaaata tggcaggtgt
    32341 tcgtaagttg caaccaaact tgcgtgttca gccaatggtt atcgatccat ttgctatcaa
    32401 cgaactagac tattacttag tttcacactt ccacagtgat catatcgacc catacacagc
    32461 tgcagcaatt ctcaacaatc ctaagttaga gcatgttaag tttatcggtc cttaccactg
    32521 tggacgaatc tgggaaggat ggggtgttcc aaaagaacgt atcatcgttg ttaaaccagg
    32581 tgacactatc gaattaaaag atatgaagat tcatgcagta gaatcatttg accgtacttg
    32641 cttggtaact ctcccagtga acggtgctga tgagacaggc ggtgaacttg ctggcttggc
    32701 tgttacagat gaagaaatgg ctcaaaaggc tgttaactat atctttgaaa caccaggtgg
    32761 aaccatctat catggtgcag attctcactt ctcaaactat tttgcaaaac atggtaaaga
    32821 ctttaaaatt gatgttgctt tgaataacta tggtgaaaat ccggtaggta tccaagacaa
    32881 aatgacatct atcgaccttc ttcgtatggc agaaaatctg cgtaccaaag tcattatccc
    32941 agttcactat gatatctggt ctaacttcat ggcttctact aatgagattc tagaactttg
    33001 gaaaatgcga aaagatcgct tgcaatacga tttccatcca tttatctggg aagttggcgg
    33061 taagtacact tatcctcaag atcaacactt agtagaatac catcatccac gtggttttga
    33121 tgattgtttt gaacaagact ctaacattca atttaaagct ttgctataat ataaaaatat
    33181 tctttggagg ttatccgtat gttgcatgat acggataatt ttcatataat agtaaaatag
    33241 aatgtgtgat tcaataatca cctcaaatag aaaggaaatt ctatgtcaaa tctatctgtt
    33301 aatgcaattc gttttctagg tattgacgcc attaataaag ccaactcagg tcatccaggt
    33361 gtggttatgg gagcggctcc gatggcttac agcctcttta caaaacaact tcatatcaat
    33421 ccagctcaac caaactggat taaccgcgac cgctttattc tttcagcagg tcatggttca
    33481 atgctccttt atgctcttct tcacctttct ggttttgaag atgtcagcat ggatgagatt
    33541 aagagtttcc gtcaatgggg ttcaaaaaca ccaggtcacc cagaatttgg tcatacggca
    33601 gggattgatg ctacgacagg tcctctaggg caagggattt caactgctac tggttttgcc
    33661 caagcagaac gtttcttggc agccaaatat aaccgtgaag gctacaatat ctttgaccac
    33721 tatacttacg ttatctgtgg agacggagac ttgatggaag gtgtctcaag cgaggcagct
    33781 tcatacgcag gtttgcaaaa acttgataag ttggttgttc tttatgattc aaatgatatc
    33841 aacttggatg gtgagacaaa ggattccttt acagaaagtg ttcgtgaccg ttacaatgcc
    33901 tacggttggc atactgcctt ggttgaaaat ggaacagact tggaagccat ccatgctgct
    33961 atcgaaacag caaaagcttc aggcaagcca tctttgattg aagtgaagac ggttattgga
    34021 tacggttctc caaacaaaca aggaactaat gctgtacacg gcgcccctct tggagcagat
    34081 gaaactgcat caactcgtca agccctcggt tgggactacg aaccatttga aattccagaa
    34141 caagtatatg ctgatttcaa agaacatgtt gcagaccgtg gcgcatcagc ttatcaagct
    34201 tggactaaat tagttgcaga ttataaagaa gctcatccag aactggctgc agaagtagaa
    34261 gccatcatcg acggacgtga tccagtcgaa gtgactccag cagacttccc agctttagaa
    34321 aatggttttt ctcaagcaac tcgtaattca agtcaagatg ccttgaatgt tgtggcagct
    34381 aaattaccaa ccttcctagg tggatcagct gaccttgctc actcaaacat gacttatatc
    34441 aagacggatg gacttcaaga cgataccaat cgcttgaatc gtaacattca gtttggtgtt
    34501 cgtgaatttg caatgggaac aatcttgaac gggatggccc ttcatggtgg acttcgtgta
    34561 tacggtggaa ctttcttcgt cttctctgac tatgtgaagg cagctgtccg cttgtcagcc
    34621 ttacaaggac ttcctgtgac ttatgtcttt acccatgatt caatcgcagt tggggaagat
    34681 ggtccgactc acgaaccagt tgagcattta gcaggtcttc gtgctatgcc aaatctaaat
    34741 gttttccgtc cagcagatgc gcgtgaaacg caagcagctt ggtaccttgc agtgacaagt
    34801 gagaaaacac caactgccct tgtcttgaca cgtcaaaact tgactgttga agatggaaca
    34861 gacttcgaca aggttgctaa aggtgcttat gttgtatatg aaaatgcagc cgactttgat
    34921 accatcttga ttgcgacagg ttcagaggtt aatcttgctg tctcagctgc taaagaattg
    34981 gctagtcaag gcgaaaaaat ccgcgtagtc agcatgccat ctacagatgt ctttgataaa
    35041 caagatgcag cttacaagga agagattctt ccaaatgcag tccgccgtcg tgttgcagtc
    35101 gaaatgggtg caagtcaaaa ctggtacaaa tatgttggtc tcgatggtgc cgttctaggt
    35161 attgatacct tcggagcctc tgccccagca ccaaaagtat tggcagaata tggctttact
    35221 gtagaaaatc ttgtaaaagt tgttcgaaac ttgaaataat cctaaaaatc agggcgtaag
    35281 ctctggtttt tcttaccaga aaagtaaggt acaatcttgt aaaagtagct gaaatttgat
    35341 atagtagtcc tatgtaaaag acaaaggaga aaacaatgaa tccaaatatt acttttttaa
    35401 tcatgcttgt aggtatgatg gccttgatgt tctttatgca acgttctcaa aagaaacaag
    35461 ctcaaaagcg tatggaaagc ttaaataaac tacaaaaagg ctatgaagtg attacaatcg
    35521 gtggacttta tggaacagtc gatgaagtag atacggagaa gggaacaata gttcttgacg
    35581 tagatggagt ttacttgact tttgaactag ctgctatcaa gacagtatta ccgctgaaag
    35641 aaacagcttc actagaaggc gcaattgaaa aataagacgg gatcacgaac tcccgttttt
    35701 ctataaaaga aaggaaatgg gatgaaaaaa ttagtctttg tctgtctggg aaatatttgc
    35761 cgtagcccta tggccgagtt tgttatgaaa tcaatgacag ataactacga aatccaaagt
    35821 cgagcaactt cctcttggga acatggcaat ccgattcata aggggactca gggaattttt
    35881 caagagtatg agattcctta tgacaagaac aagacatcgc ttcagattag taaggaagat
    35941 tttgaagcct ttgattatat tatcggaatg gacgcttcaa atatttctga cttacgtcag
    36001 atgtgtccag tagactgtca agataagatt tactcatttt catctgaaag tgttccagac
    36061 ccttggtata caggagattt tgaagaaacc tatcgacgtg ttcaagaggg ctgtcaagct
    36121 tggttagaac gtttagaaaa ggagagttaa ggtggaaaat cttacgaatt tttacgaaaa
    36181 gtatcgtgtc tatctgactc gtccacgatt agagcttttg gcagtagtta ccattgtttt
    36241 ctgtgctgta ctcgtctttt ttctaaatat tccaggaaaa ggtgtcttaa aactcgataa
    36301 tggaacgatt gtttatgatg gcagtcttgt ccgtggtaaa atgaatggtc aaggtaccat
    36361 taccttccaa aatggagacc aatatacagg tggcttcaac aatggagcct tcaacggaaa
    36421 aggtaccttt caatctaaag aaggctggac ctacgaaggt gattttgtaa atggtcaggc
    36481 tgaaggaaaa gggaaactaa caacagaaca agaagtcgtt tatgaaggaa cttttaaaca
    36541 aggcgttttt caacaaaaat aaagcctcct tatcaaagga ggtattatta gaattacaag
    36601 gtaagcgctt acctgtaaat ccctttcttt ccaaatccct cttccaagca agtttgtgaa
    36661 ataaaaaata tttgaaataa atttcacaaa cttcaaagat aaaacctgat aagaaaagaa
    36721 aatgagaaaa gtttcacaag agtttaaaaa ttctttgtga aatgtttatg aaactgttta
    36781 caaagttgaa ataatacgtt ataataaact tgtgaaaaaa ttaacaaagg gtatcgatcc
    36841 ttgaaagcta tggaggaaaa tatggctgat aaaaaaactg tgacaccaga ggaaaagaaa
    36901 ctcgttgctg aaaaacacgt agatgagttg gttcaaaaag ctctagttgc ccttgaagaa
    36961 atgcgtaaat tggatcaaga gcaagttgac tacatcgttg ccaaagcatc agtagcagct
    37021 ttggatgccc acggagaatt ggctttacat gcctttgaag aaacaggacg tggtgtattt
    37081 gaagataaag caactaagaa cttgtttgcc tgtgaacacg tagtaaacaa catgcgtcac
    37141 actaagacag ttggcgttat cgaagaagac gatgtaacag gattgactct tattgctgaa
    37201 ccagttggtg ttgtttgtgg tattactcca acaacaaacc caacatcaac agcaatcttc
    37261 aaatcattga tttcattgaa gacacgtaac ccaatcgtct ttgccttcca tccatcagca
    37321 caagaatcat ctgctcatgc agctcgtatc gtccgcgatg cagctatcgc agctggtgct
    37381 cctgaaaact gtgtgcaatg gattactcaa ccatctatgg aagcaacaag tgcccttatg
    37441 aaccacgaag gtgttgcgac aatccttgca acaggtggta atgccatggt taaggcggct
    37501 tattcatgtg gtaaaccagc tcttggggta ggtgccggaa acgttccagc ttatgttgaa
    37561 aaatcagcaa acattcgtca agcagcacac gatatcgtca tgtctaaatc atttgataac
    37621 ggtatggtct gtgcatctga acaagcagtt atcattgata aagaaattta cgatgaattt
    37681 gtagcagagt tcaaatctta ccacacttac tttgtaaaca aaaaagaaaa agctcttctt
    37741 gaagagttct gcttcggcgt caaagcaaac agcaaaaact gtgctggtgc aaaattgaac
    37801 gctgacatcg ttggtaaacc agcaacttgg attgcagaac aagcaggatt tacagttcca
    37861 gaaggaacaa acattcttgc tgcagaatgt aaagaagttg gcgaaaatga gccattgact
    37921 cgtgaaaaat tgtcaccagt tattgcagtt ttgaaatctg aaagccgtga agatggtatt
    37981 actaaggctc gtcaaatggt tgaatttaac ggtcttggac actcagcagc tatccacaca
    38041 gctgacgaag aattgactaa agaatttggt aaagctgtta aagctattcg tgttatctgt
    38101 aactcacctt ctacttttgg tggtatcggg gacgtttaca atgccttctt gccatcattg
    38161 acacttggat gtggttctta cggacgcaac tcagttgggg ataacgttag tgccattaac
    38221 ctcttgaata tcaaaaaagt cggaagacgg agaaataaca tgcaatggat gaaacttcct
    38281 tcaaaaacat actttgaacg tgattcaatt caataccttc aaaaatgtcg tgacgttgaa
    38341 cgtgtcatga tcgttactga ccatgccatg gtagagcttg gtttccttga tcgtatcatc
    38401 gaacaactgg accttcgtcg caataaggtt gtttaccaaa tctttgcgga tgtagaaccg
    38461 gatccagata tcacaactgt aaaccgtggt actgagatta tgcgtgcctt caaaccagat
    38521 accatcatcg cactcggtgg tgggtctcca atggatgctg ccaaagtaat gtggctcttc
    38581 tacgagcaac cagaagtgga cttccgtgac cttgtccaaa aattcatgga tatccgtaaa
    38641 cgtgccttca agttcccatt gcttggtaag aagactaaat tcatcgcgat tccaactaca
    38701 tctggtacag gatctgaagt aacaccattt gccgttatct ctgataaagc aaacaaccgt
    38761 aaatacccaa tcgctgacta ctcattgaca ccaactgtgg caatcgtaga tcctgctttg
    38821 gtattgacag ttccaggatt tgttgctgct gatactggta tggacgtatt gactcacgcg
    38881 acagaagcat acgtatcaca aatggctagt gactacactg atggtttagc acttcaagcc
    38941 attaaattgg tctttgaaaa tctcgaaagc tcagttaaga atgcagactt ccactcacgt
    39001 gagaaaatgc ataacgcttc aacaatcgct ggtatggcct ttgccaatgc cttcctaggt
    39061 atttctcact caatggccca taagattggt gcgcaattcc acacaatcca cggtcgtaca
    39121 aatgctatct tgcttccata cgttatccgt tacaacggta cacgtccagc taagacagca
    39181 acatggccta agtacaacta ctaccgtgca gatgaaaaat accaagatat cgcacgcatg
    39241 cttggacttc cagcttctac tccagaagaa ggggttgaat cttacgcaaa agctgtctac
    39301 gaactcggtg aacgtattgg gatccaaatg aattttagag accaaggaat tgacgaaaaa
    39361 gaatggaaag aacattctcg tgaattagcc ttcctggctt atgaagacca atgttcacca
    39421 gctaacccac gtcttccaat ggtagaccat atgcaagaaa tcatcgaaga tgcatactat
    39481 ggctacaaag aaagaccagg acgccgtaaa taattgttta tcagtctaga agcaagacaa
    39541 aaactcaatt tgagggaaag atccagtaat ttttctatga taaaaggcat cctatcaagg
    39601 tttttgaaca cctgatagga tgccttttta tgatattgag gcctttttgc cctttttgaa
    39661 aaactagaat agaaacaaaa tatataatag attgaaacta gagtagtaca catctgcttc
    39721 taaaacattg ttagaattcg atttga
//
LOCUS       DBERMR010000014        39735 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.014, whole genome shotgun sequence.
ACCESSION   DBERMR010000014 DBERMR010000000
VERSION     DBERMR010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 39735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39735
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.014"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            181..519
                     /locus_tag="ACL8A1_000791"
     CDS             181..519
                     /locus_tag="ACL8A1_000791"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HGS1201593.1"
                     /translation="MTLTIHVYYKGDGDSAIKFAREMLDSGLVEEIRNQKGNLKYEYF
                     LPIEKEGTILLIDQWINQEALDEHHQSKTMQKILDLRKKYHLQMQVERYIEDDSGMPE
                     SDKSFIDTGN"
     gene            709..2180
                     /locus_tag="ACL8A1_000792"
                     /pseudo
     CDS             709..2180
                     /locus_tag="ACL8A1_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206493.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
     gene            2184..2423
                     /locus_tag="ACL8A1_000793"
     CDS             2184..2423
                     /locus_tag="ACL8A1_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HGS1201594.1"
                     /translation="MIGSIAAILTTFAFLPQVFRIVKTKDTGSIALGMYVMQVIDIAL
                     WLAHGIRIGDLPLILANSVSFLLSGIILFYKLKYK"
     gene            2553..2867
                     /locus_tag="ACL8A1_000794"
     CDS             2553..2867
                     /locus_tag="ACL8A1_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HGS1201595.1"
                     /translation="MAKALIICAAGMSSSLMAQKTTEFLKGKGKDIVVDAISATEGGK
                     AIAAAAFDLYLVSPQTKMYFKQFEEAGSKAGKPVVQIPPQAYIPIPMGIEKMANLILE
                     NI"
     gene            2985..4964
                     /locus_tag="ACL8A1_000795"
     CDS             2985..4964
                     /locus_tag="ACL8A1_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGS1201596.1"
                     /translation="MNVLNIKEKDILQFLIKNKERFVTSKELAEYLSCSDRTVRNVLK
                     LIEKTMIIQGVRLISKQGQGYQIFFENQGAYQEFRQTYELEEDYTKTAVSKGDDRLVF
                     ILNKLLFEQVPVLFDDLADELYVSRSTLSHDFRKIRVMLSEYNLSIESRANKGVYVSG
                     EERDKRRFIINYFLENQFFKTIHCYVKFNFFDQTVPLEEFARIVLDECQEANLKLSDF
                     VLQNLVVHIALSVIRLKSGFEIKNIDCQMTDDATERKVAQRILSKVREVTNQEFPVQE
                     IDYITLHLLAKSQQCQKNQKNISEEVLKKSLFKTFQNLGLDDMYNFSSDFQLIEGLIT
                     HLMTLQVRLESRITLNNPLVDEIKQNYSDIFFMTREILANMDKFLEWTISDDEVAYVS
                     LHFLAAMERSKESTKFNILAICATGFGAAQMLRNRLETEFGKRVEVVDVIGYYELNQE
                     KLKGIDFIVSAVDLSNLYFQIPVFKVSVFLKSDEMEMIRKAMDQMQVSSYVQSSKINK
                     FENNSFRQYFSKENFLICTESDKVNLLEKMVESLSVGESNEFEQSLLYGIKQREELSS
                     VVFSEKIAVPHPIQPFGTEGKVSVAICKDSLLWDNQSSHVQLVFLLSPSIYGNEGLAT
                     VTKKIVSLTENDELQNQLISCNNFEDFINIFEKIK"
     gene            4974..5288
                     /locus_tag="ACL8A1_000796"
     CDS             4974..5288
                     /locus_tag="ACL8A1_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HGS1201597.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAKEMLALYKK
                     VN"
     gene            5319..5825
                     /locus_tag="ACL8A1_000797"
     CDS             5319..5825
                     /locus_tag="ACL8A1_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201598.1"
                     /translation="MDKKERQKIEQQRREMALTNTFFNRYLLLRYSIALFFFGNIYWL
                     LNQFISPSPIIIFPIMLIVFSILATIEQFKLYGNRKEKLGITLMFVRIQILISIGLLV
                     LTWTSWFKNLFPIFENNQVARLFVFVVLLLGLVLSLLDIRRIKKIYKRTDKAYQQFVQ
                     LEKNSLSL"
     gene            5905..7251
                     /gene="celB"
                     /locus_tag="ACL8A1_000798"
     CDS             5905..7251
                     /gene="celB"
                     /locus_tag="ACL8A1_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HGS1201599.1"
                     /translation="MDQQNGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFTSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNPLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NGVARFGTTGIFTAIIMAIVTVLIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVV
                     AFVVIFINGLLVAMGTDIFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGAN
                     IVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQL
                     KAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPI
                     IAQVPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
     gene            7631..7807
                     /locus_tag="ACL8A1_000799"
     CDS             7631..7807
                     /locus_tag="ACL8A1_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201600.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            8208..10421
                     /locus_tag="ACL8A1_000800"
     CDS             8208..10421
                     /locus_tag="ACL8A1_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HGS1201601.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSQTGSFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(10643..11119)
                     /locus_tag="ACL8A1_000801"
     CDS             complement(10643..11119)
                     /locus_tag="ACL8A1_000801"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HGS1201602.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLQDQKSGPLGKRIEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            11342..14545
                     /locus_tag="ACL8A1_000802"
     CDS             11342..14545
                     /locus_tag="ACL8A1_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HGS1201603.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSSADASTRVIEAKDG
                     AITISSPEKLRAAVHRMVPIEAKKKYKLRFKIKTDHKVGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQLSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKE
                     VKKIPLKILASVKDAYTDRLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WMHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTVMYNAERGFGF
                     GLSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWSTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVDRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            14898..15274
                     /gene="tnpA"
                     /locus_tag="ACL8A1_000803"
                     /pseudo
     CDS             14898..15274
                     /gene="tnpA"
                     /locus_tag="ACL8A1_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775157.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            15456..15518
                     /locus_tag="ACL8A1_000804"
                     /pseudo
     CDS             15456..15518
                     /locus_tag="ACL8A1_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596946.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(15662..16291)
                     /locus_tag="ACL8A1_000805"
     CDS             complement(15662..16291)
                     /locus_tag="ACL8A1_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HGS1201604.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYVTLR"
     gene            complement(16301..17302)
                     /locus_tag="ACL8A1_000806"
     CDS             complement(16301..17302)
                     /locus_tag="ACL8A1_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HGS1201605.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDIDSLFQGISHFHFSGITVAIGQEVRTILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEELFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(17333..17974)
                     /locus_tag="ACL8A1_000807"
     CDS             complement(17333..17974)
                     /locus_tag="ACL8A1_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HGS1201606.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKGYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQIAHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(17993..18808)
                     /locus_tag="ACL8A1_000808"
     CDS             complement(17993..18808)
                     /locus_tag="ACL8A1_000808"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HGS1201607.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
     gene            19080..19514
                     /locus_tag="ACL8A1_000809"
     CDS             19080..19514
                     /locus_tag="ACL8A1_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGS1201608.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFVR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            19526..20716
                     /locus_tag="ACL8A1_000810"
     CDS             19526..20716
                     /locus_tag="ACL8A1_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HGS1201609.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            20727..21218
                     /locus_tag="ACL8A1_000811"
     CDS             20727..21218
                     /locus_tag="ACL8A1_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS1201610.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            21233..22012
                     /locus_tag="ACL8A1_000812"
     CDS             21233..22012
                     /locus_tag="ACL8A1_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HGS1201611.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            21999..22817
                     /locus_tag="ACL8A1_000813"
     CDS             21999..22817
                     /locus_tag="ACL8A1_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGS1201612.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPTKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            22817..23110
                     /gene="yajC"
                     /locus_tag="ACL8A1_000814"
     CDS             22817..23110
                     /gene="yajC"
                     /locus_tag="ACL8A1_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HGS1201613.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            23132..25033
                     /locus_tag="ACL8A1_000815"
     CDS             23132..25033
                     /locus_tag="ACL8A1_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HGS1201614.1"
                     /translation="MKEERRQFFERVDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWSYMLNRQEYLLQFMIGYLVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSYYRAKYTLSNWGILQTIPMLAIYHFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEMLSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     RRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGMHY
                     IEGAYWSAEPDLPYLHRRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDIGVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            25093..26094
                     /locus_tag="ACL8A1_000816"
     CDS             25093..26094
                     /locus_tag="ACL8A1_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGS1201615.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(26204..26539)
                     /locus_tag="ACL8A1_000817"
     CDS             complement(26204..26539)
                     /locus_tag="ACL8A1_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201616.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(26550..26792)
                     /locus_tag="ACL8A1_000818"
     CDS             complement(26550..26792)
                     /locus_tag="ACL8A1_000818"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201617.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            complement(26808..27002)
                     /locus_tag="ACL8A1_000819"
     CDS             complement(26808..27002)
                     /locus_tag="ACL8A1_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS1201618.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWKEDF"
     gene            27168..28118
                     /gene="rsmH"
                     /locus_tag="ACL8A1_000820"
     CDS             27168..28118
                     /gene="rsmH"
                     /locus_tag="ACL8A1_000820"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HGS1201619.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            28130..28447
                     /gene="ftsL"
                     /locus_tag="ACL8A1_000821"
     CDS             28130..28447
                     /gene="ftsL"
                     /locus_tag="ACL8A1_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HGS1201620.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            28451..30703
                     /gene="pbp2X"
                     /locus_tag="ACL8A1_000822"
                     /gene_synonym="pbpX"
     CDS             28451..30703
                     /gene="pbp2X"
                     /locus_tag="ACL8A1_000822"
                     /gene_synonym="pbpX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HGS1201621.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGVT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKETAETFAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            30705..31685
                     /gene="mraY"
                     /locus_tag="ACL8A1_000823"
     CDS             30705..31685
                     /gene="mraY"
                     /locus_tag="ACL8A1_000823"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HGS1201622.1"
                     /translation="MFISISAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGIIFYLFYERGGDILSVFGYPVHLGFFYIFFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            31899..32039
                     /locus_tag="ACL8A1_000824"
     CDS             31899..32039
                     /locus_tag="ACL8A1_000824"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201623.1"
                     /translation="MVDRTDEVSSKHGFEVVDKAKLMWFEEIFEECKKYWSVKVKILD
                     LF"
     gene            32295..34400
                     /locus_tag="ACL8A1_000825"
     CDS             32295..34400
                     /locus_tag="ACL8A1_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGS1201624.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEDLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSQLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     TKHLEADMEDGVLVIREKA"
     gene            34852..35195
                     /locus_tag="ACL8A1_000826"
                     /pseudo
     CDS             34852..35195
                     /locus_tag="ACL8A1_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
     gene            complement(35262..35744)
                     /locus_tag="ACL8A1_000827"
     CDS             complement(35262..35744)
                     /locus_tag="ACL8A1_000827"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HGS1201625.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(35839..37323)
                     /locus_tag="ACL8A1_000828"
     CDS             complement(35839..37323)
                     /locus_tag="ACL8A1_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HGS1201626.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFLNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            37476..39083
                     /locus_tag="ACL8A1_000829"
     CDS             37476..39083
                     /locus_tag="ACL8A1_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HGS1201627.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGIGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIATIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENPNSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDENKNAGFSTGQPWLAVNLNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLIRADFELLDTVDKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
     gene            39242..39406
                     /locus_tag="ACL8A1_000830"
                     /pseudo
     CDS             39242..39406
                     /locus_tag="ACL8A1_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093127.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
     gene            complement(39408..>39735)
                     /locus_tag="ACL8A1_000831"
     CDS             complement(39408..>39735)
                     /locus_tag="ACL8A1_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGS1201628.1"
                     /translation="LTNGELIVPMTYEETMTSDFFEAWFQKFLLPTLTTPSVIIMDNA
                     RFHRMGKLELLCEEFGHKLLPLPPYSPEYNPIEKTWAHIKKHLKKVLPSYNTFYEVLL
                     SCSCFN"
BASE COUNT        12430 a         6694 c         8648 g        11963 t
ORIGIN      
        1 tttgtcctgc tcttgtttca attgactata aatcgctata agaaaaaatc attttattgg
       61 tataaagaag tgattgaaag taatggcgaa acgttagata attagttcat aaaaaacaag
      121 tttagatcct catctaaact tgttttttta aaattgaagg atgattttga aggatataag
      181 atgacattga ctattcatgt atattataaa ggtgatggag atagtgcaat aaagtttgca
      241 agagaaatgc ttgatagtgg ccttgttgaa gaaattagaa atcaaaaagg taacttgaaa
      301 tacgaatatt ttttaccaat tgaaaaagag ggaactattc tccttattga tcagtggata
      361 aatcaagaag ctttagatga acatcatcaa tcaaagacaa tgcagaaaat cttagattta
      421 agaaaaaaat atcacttgca gatgcaagtt gaacgttata tagaagatga ttctgggatg
      481 ccagaaagcg ataagtcgtt tatagacaca gggaattaga attattgtga gagtttttgt
      541 agtttgaaag tattgaaaat cgtaaagata tcattacgag aatacaagaa gatattgttc
      601 tatttccatt aaaaattact tctttcctta acaataagga aaaagaatgc gttttcatct
      661 tttggtttga ggtttataat aagagtatca aagtcaggag gaaaaaagat gactactttt
      721 aaagatggat ttttatgggg tggtgctgtt gctgctcatc aacttgaagg tggatggcaa
      781 gaaggtggca agggaattag tgttgctgat gttatgactg ctggtcgtca tggagtagct
      841 cgtgaaatta cttcgggagt tttagagggt aaatattatc caaatcatga ggcgatagat
      901 ttttatcatc gttataaaga agacatagca ctttttgctg aaatgggatt caagtgcttc
      961 cgtacctcta ttgcatggac gcgtatcttt ccaaaaggtg atgagttaga gccgaatgaa
     1021 gaaggattac agttttatga taatcttttt gatgaatgct taaagaatgg tattgaacct
     1081 gtcatcactc tatctcattt tgaaatgcct tatcacttag tgaccgaata tggtggttgg
     1141 aaaaatagga aattgattga tttctttgct cgttttgcag aagtcgtatt taaacgttac
     1201 aaagataagg ttaaatattg gatgactttc aatgaaatca ataatcaagc gaattatcag
     1261 gaagattttg caccatttac taactcaggt attgtatatg aggaaggtga taatagagaa
     1321 gcaattatgt atcaagcagc acattacgaa ttagttgctt ctgcacgagc tgtaaaaatt
     1381 ggtcatgaga ttaatccaga ttttcaaata ggttgtatcc aatttatcca gctacttgca
     1441 atcctaagga tatcttaatg gcaatgaaag ctatgcagaa gcgttattat tttgctgatg
     1501 tgcatgtttt aggtaaatat cctgagcata ttttcaagta ttgggaacga aaaggtattt
     1561 cagttgattt tgctgcccag gataaagaag atttacttgg tgggactgta gattacattg
     1621 gtttcagtta ctatatgtcc tttgctatcg actctcatcg tgaaaataat ccttattttg
     1681 attatcttga aacagaagat ttagtgaaaa ataattatgt taaggcttct gaatgggagt
     1741 ggcaaattga tccagaaggt ttacgttatg cgttaaattg gtttacagac cactatcact
     1801 taccactctt tattgttgaa aatggttttg gagctataga tcaagttgca gcagatggta
     1861 tggtacatga tgattataga attgaatatc taggtgccca tattcgtgaa atgaaaaagg
     1921 ctgtagttga agatggtgtt gatttaatgg gttatactcc atggggatgt attgatttgg
     1981 tttcagctgg taccggtgaa atgcggaaac gttatggttt tatttatgta gataaagatg
     2041 acaatgggaa gggaagttat aatcgttccc cgaaaaaatc ttttggctgg tataaggaag
     2101 ttatttcatc taacggtgaa tcagtagaat atgctaaaca aactgagatt attctcagtt
     2161 tgtttagcat attgggatga catatgattg gaagtattgc agcaatttta acaacatttg
     2221 catttttacc acaagttttt cgtatagtta agacaaagga cacaggatct attgctctag
     2281 gtatgtatgt tatgcaagtt attgatattg cattgtggct tgctcatggt attcgtatag
     2341 gagacttgcc tttgatttta gcaaatagtg tttcatttct actctcagga ataattttat
     2401 tttataaatt gaagtataaa taattttttt ccatatcatt taggaaaatg agggaattgt
     2461 cattggaaac gctttatttt ataataaaaa tagataacag ataagtcaac ttagcacagc
     2521 tttgattgga tttaagaaaa ggagaaaata ttatggcaaa agctttaatt atttgtgcag
     2581 caggaatgtc ttcatcactt atggcacaga aaacaacaga gtttctaaaa ggaaaaggga
     2641 aagatattgt agttgatgct attagtgcta ctgaaggagg gaaagctatc gcagcagcag
     2701 cttttgattt gtatcttgtc agccctcaaa caaaaatgta ctttaagcaa tttgaagagg
     2761 ctggttctaa agcaggaaaa ccagttgtac aaatacctcc tcaagcctat attcctattc
     2821 caatgggaat tgaaaaaatg gctaatttga ttcttgagaa tatttgatga acacatgaaa
     2881 tgtcaataaa tatttaagtt aagataaatt gttttgagtt tgatataaat tgatttaagt
     2941 gtataatatg cttcaatata tgaaaacata cgttttagga gggaatgaat gtgttaaaca
     3001 ttaaagagaa agatatcctt caatttttaa taaaaaataa ggaacgtttt gttacaagca
     3061 aagaactggc agaatattta tcctgttcag atagaactgt ccgtaatgta ctgaaattaa
     3121 ttgagaagac aatgattatt caaggagtcc ggttgatttc aaaacaaggt caagggtatc
     3181 aaatattttt tgagaatcaa ggcgcttacc aggagtttag acaaacttat gaactggaag
     3241 aagactatac gaaaactgcg gtttcaaaag gtgatgatcg tttagtattt atcttaaata
     3301 aattattatt tgagcaagtt ccagttcttt ttgatgattt agctgatgaa ctttacgtta
     3361 gccgttcaac attatctcat gatttcagaa agatacgagt gatgttatca gagtataatt
     3421 tgtccattga gagtagggcg aataaaggag tttatgtctc aggcgaggaa cgtgataaac
     3481 gacgttttat cataaattat ttcctagaga atcaattttt taaaacaata cattgctatg
     3541 taaaatttaa cttttttgat caaactgttc cattagaaga atttgctaga attgttttgg
     3601 atgaatgcca agaggccaac ctaaaactat ctgattttgt actacagaac ttagtagttc
     3661 acatagcatt gtctgtgatt cgtttaaaat ccggatttga aataaaaaat atagactgtc
     3721 agatgactga tgatgcaaca gagagaaagg ttgcccaaag aattctttct aaagtaagag
     3781 aagtaacaaa tcaggaattt ccagttcagg aaatagacta cattactttg catttgttag
     3841 ctaagagtca acaatgtcaa aaaaatcaga agaatatttc tgaagaagtt ttaaaaaaat
     3901 ctttatttaa aacatttcag aatttaggat tagatgatat gtataacttt tcttcagatt
     3961 tccaactaat tgaaggattg ataactcatc taatgacact acaagtacgt ttagaaagcc
     4021 ggattacctt aaataaccct ttagtagatg aaattaaaca gaattatagt gatatattct
     4081 ttatgactag agaaattctt gcaaatatgg acaagttctt ggagtggaca atatcagatg
     4141 atgaagtagc atatgtaagt cttcactttt tagctgctat ggaacgtagc aaagagagta
     4201 ctaagtttaa tattcttgct atttgtgcaa caggatttgg tgcagcccaa atgttaagaa
     4261 atcgtttgga aacggagttt gggaagcgag tagaagttgt tgatgtaata ggctattatg
     4321 aattgaatca agaaaaactt aaaggaatag attttatagt gtcagctgtt gatttatcaa
     4381 atctatattt tcaaatccca gtttttaaag ttagtgtgtt tcttaagagt gatgagatgg
     4441 aaatgattag aaaagctatg gatcagatgc aagtttctag ttatgtacaa tcatcaaaga
     4501 ttaataaatt tgaaaacaac agctttagac agtattttag taaagaaaat tttttaatat
     4561 gtactgaatc agacaaggta aatttacttg aaaagatggt tgagagttta tctgttggtg
     4621 aatcaaatga gtttgaacaa tctttacttt atggaattaa acagcgcgaa gaattaagtt
     4681 cagttgtttt ttctgaaaaa attgcagttc cgcatcctat tcagcccttt ggtacagagg
     4741 gaaaggtttc ggttgcaatt tgtaaagatt cgttactctg ggataatcaa agttcacatg
     4801 tccagctagt atttcttcta tctccatcaa tatatgggaa cgaaggtttg gcaactgtga
     4861 ctaaaaagat agtctctctg acagagaatg atgaattgca aaatcagcta atatcttgca
     4921 ataactttga agattttatt aacatatttg agaagataaa ataggaggtt catatgaatc
     4981 aagaggaact gcaggttgca gcatttgaaa ttatcctgca ttcgggaaat gcaaggtcag
     5041 aaatacatga agcatttgca aaaatgagag aaggtagttt tgatgatgca gaaagtaaat
     5101 taaatcaatc aaatgagata attcttgaag cgcatcatgc acaaaccaaa ctcttgcaag
     5161 aatacgcaag tggagtggaa attaaaattg aaattatcat ggtacatgct caagatcatt
     5221 taatgaccac tatgacatta ttagaagtag caaaggaaat gttagctctt tataagaaag
     5281 ttaactaaat atgtgttgtt atataaggag gaaatgatat ggataaaaaa gaacgacaaa
     5341 agattgaaca acaacgtcga gagatggctc taactaatac tttttttaat cgatatcttc
     5401 ttctacgtta ttcaatagca ctcttctttt ttgggaatat ttattggctt ttgaatcagt
     5461 ttattagtcc ctcgcctatt attattttcc ccattatgct catagtattt tctattttag
     5521 caactattga acaatttaaa ctttatggaa ataggaaaga aaagttggga ataacactaa
     5581 tgtttgttag aatacaaata cttatttcaa taggattact tgttttaact tggacaagtt
     5641 ggtttaagaa tctttttccg atttttgaaa ataaccaagt agcacgttta tttgtttttg
     5701 tagttcttct attaggttta gttctgagtt tgttagacat tagaagaatt aaaaaaattt
     5761 ataaacgaac agataaggct tatcaacaat ttgtccaatt ggaaaagaac tcacttagtt
     5821 tataatcctg atctagtata atttgtattt gaaactagtc tttagaaagt aaaaattatt
     5881 atgttttatt aaggagttct atttatggat caacaaaacg ggttgtttgg ttttcttgaa
     5941 aaccatgtta tgggaccaat gggcaaactt gctcagttta aagtagtgcg tgctatcacg
     6001 gctgcaggta tggctgctgt accatttact attgtaggat caatgttttt ggtattcagt
     6061 attttgccac aagctttctc attttggcca attgtggcag atattttctc tgcttcattt
     6121 gataaattca catcacttta catggttgca aactatgcga ctatgggttc tctatctctt
     6181 tatttcgttc tatcacttgc atatgaattg acaaaaattt atgcagagga agaagaactc
     6241 aatatgaatc ctcttaatgg tgccttgctt gccttgatgg cttttgtcat gacagtaccg
     6301 caaatcattt ttgatggtgg aatgatgaag actgtgacaa gtctaaaaga aggtgcagta
     6361 attgcagatg gatgggcaat gggaaatgga gtcgcacgtt ttgggacaac agggattttt
     6421 accgcaatca ttatggcaat tgtgactgtt cttatttatc gtatgtgtgt taaacataat
     6481 tgggttatta aaatgcctga agctgttcca gaaggagttt ctcgtggatt taccgctttg
     6541 gttccgggat ttgttgttgc atttgttgtt atctttatca acggtcttct tgtagcaatg
     6601 ggaacagata tttttaaagt cattgcaatt ccatttggtt ttgtatccaa tctgactaat
     6661 tcgtggattg gtttaatgat tatttatcta ttgactcaac tactttggat tgtaggtatc
     6721 cacggtgcga acattgtttt tgcatttgtt agtccaattg ctcttgctaa catggctgaa
     6781 aatgctgctg gcgggcactt cgctgttgca ggtgaatttt ctaatatgtt tgtaattgca
     6841 ggtggttctg gtgcaacttt aggactatgt ttatatattg cttttgcctc taaatctgaa
     6901 cagcttaaag caataggacg agcatctgta gttccggcct tatttaatat taatgaacca
     6961 ttaatttttg gattacctat tatctataat ccagccttgg ctataccatt tattttagca
     7021 ccaatggtta ctgctactat ttattacgta gcgaattctc taaactttat taagccaatt
     7081 atcgcacagg ttccatggcc aactccagta gggattggag ctttcttagg gacagcagat
     7141 cttcgagctg tattagttgc tctagtatgt gcatttgcag cattcctagt ctatcttcca
     7201 ttcatccgtg tatatgatca aaaattggtg aaagaagagc aaggtatcta ataagtaaaa
     7261 aagtatactc gatgatagaa tccatatagt ttaaataatg agtgttaata ttagcagtga
     7321 agaggtcttt ttaagatatt cttctcttct ttttcatatt ttttcgctat gtagggtatg
     7381 aagcaaaata gaaagtagtt cgaatagttg ataaacagga aactgctgtt atttaaaaaa
     7441 aacaataaag taaagtatgt tatcgtagaa tacgatacaa tagtaaaaaa gcaaaaagaa
     7501 tgcgttttat atttgggttt gaggtttata aaataggata agcgtgctga aaacttagat
     7561 atgacaaaca aaataaaatt tttaaaagct acatagaaac atggtataat gtatctaaag
     7621 gaggtagcat atgtccttag atatagataa agaaaagatg acaatcatgg gaatagcttt
     7681 tgaaaacaga tccgttttta agagtgtttg gtatgcttta agtacaaata tgattgaagg
     7741 atggcgacct acggttagtg atgttgaaaa attacgagat gaagctcttg ctctggggat
     7801 gacttaatga agcgcaactg ttatgagtct tatgactatg ttgatccgga taatttatat
     7861 accgatccgg gttcttcagt tctaagaaat aagcaggaag gaaaaaagca cgcgagcttg
     7921 aatatcgtat gattgctagt aagagtttga aattatttat caatcctatc ttagttaata
     7981 gtttaaaagt agtttgtttc acaactgcat aagcttagaa aagttgaaaa gtgataactc
     8041 tttaataaac tcttggtttg ttaaaagata ttttgtccac tacatttttt caattgacta
     8101 ttatttgtca tagatataac agatagaaca ttgcattcag aataatgaaa aacgcatcaa
     8161 aaatggtgcg ttctgttttt atgctaccat aaaaggagga aaacaaattg aaaattttta
     8221 agggagagtt ttatcgaatc tctgtattaa cagacaagct agtaaggtta gaatactctc
     8281 aaactggaag ttttgaggat agaacgacac aacttatcta taatagagat tttggccaag
     8341 tttcgttaga ttatatcgag acatcaaacg tactagatat tatgacggac tattttcatc
     8401 tgcactttaa taaaggagaa tttaacgccg aaaatttatt tatagaatta aaaggaaatt
     8461 ttgccgtata tggtagtcgc tggtattttg gtgaatctat tgaaacgtta aaaggaacag
     8521 ctcggactct ggataaggca gatggagcaa tctcgttaga agatggaatt attagccgaa
     8581 atggtatagc cttattggat gattctcaag gatttatttg ggatgaacaa tctggttata
     8641 ttgagagaga aaatcaaatt gacctgtatt tctttgccta tgggcatgat tatagaggag
     8701 caatcagaga cttttaccat ttgactggtt caacaccctt gttgccaaga tatgctttag
     8761 gcaattggtg gagtagatat tggccttata cgtcggatga atatttggat ttaatagaca
     8821 gatttgaaac agagaaaatt ccattatcta tcggtgtgtt agatatggat tggcatataa
     8881 ctgacattcc agctcgtttt ggaagtggct ggacaggata tagttggaat agaaacttaa
     8941 taccaaatcc agaacagtta ttgcaacaac ttcatgatag aaagttaaaa ctctccttaa
     9001 atgtccatcc tgctgatggg atacgggctt atgaagaagc ttatcctcaa atcgcaaaac
     9061 ggttggggtt aaatgtagaa ctagaagaac ctgctatttt tgattttttt aatccctctt
     9121 ttagggaagc ctactttaaa gatgttcatt atgagctaga aaagcaggga gtagattttt
     9181 ggtggattga ctggcaacaa gggacacaag gtatgctaga tccactttgg cttttaaacc
     9241 attatcacta tcaggatagt tgtaaaaatg cagaaggtgg tttgatttta tcaagatatg
     9301 caggtcctgg tagtcaccgc taccctgttg gtttttcagg ggatactatt attagttgga
     9361 attccttaag atttcaaccc tattttacag cgacagcatc taatatcggt tatagttggt
     9421 ggagtcatga tatcggtgga catatgttgg gggattatga cgaagagcta caaactagat
     9481 ggctacagtt tggtgttttt agtccgatta ctcgattaca tagttctaga agtcctttta
     9541 atagtaaaga accttggttt ttttcagaaa caacatctaa gattatgaag aaataccttc
     9601 gtttgagaca tcagatgatt ccttatctat ataccatgaa tgtaaagaca catgaggaag
     9661 gtgccccatt aatcagtcca atctattatt tctatccaga gaatgatgag agctataatg
     9721 ttccaaacca atactttttt ggaacagaac tgatggtggc tcccattgta gaaaagatgg
     9781 atttgacatt ccaatctgca aaagtagatg tatggttccc tgaaggtgaa tggtatgact
     9841 tcttttcaga gaaaaaatac acaggtggtg tgaagttaag tgtttatagg gacatctcga
     9901 ctacgcctgt gtttgcaaaa agtggtgcaa tcattccctt ggttggttct gagataggta
     9961 tgggtgttga tttacctgag gttgtagatt ggtatgtatt cccagggaaa caacattctt
    10021 ttgaaatgct tgaagatcaa aatggtcaaa gatataaaac aagattatca atcgattggg
    10081 aaatgggaat ggtagagtta gcattacaag gagattctag tatcgttcca agcaatagaa
    10141 aacatagaat tcattttaaa ggaacgaatg tgtctataat tgaattgcca aataagaatg
    10201 atacagctaa atttgaatgg aaagataata aaaggacatc tttaaatgac gaagttttta
    10261 gactactaaa gacagcttct cttccatatg aattaaaaga tagattgtta aatcaattca
    10321 tcaatgccaa aaactctcat gacttaatga atatcttgca tcatcaggat aaggaattga
    10381 gagggcgttt gttggaaatg atatttacta gccaaaacta attgaaacaa ttagaaagtc
    10441 atttgtttag tgtatatgaa gattctgtat ggtattcgtt atcactggtc acattgaaaa
    10501 tgtgaaataa gcacctacaa catagacttt acaacacttc atgtttcata tcatggggtg
    10561 tttttctatt taagtcaaag ccaatcaaaa aatctcacta ttcatcaggt taaaggaaac
    10621 ctaatcatag tgagattgtg aattatagta gagtttgtat cgcctcttca atttgttttg
    10681 ggtctgtttt tgaagaaaag cgttcaaaga cttgcccatc tcgaccgatg agaaacttag
    10741 cgaaattcca ttcgattcgt tttcctagtg ggccagattt ctggtcttgt aaccagacat
    10801 agagagggtc tgcttcctta ccgttgacct taatcttggc aaaacgtggg aaggtggttt
    10861 gaaaatgtag gctacagaag gcgttgattt cctctgcgct gccgggtgct tgtcccataa
    10921 actgattgca agggaaatcc aatatttcaa agccctgatc ttgatagcgt tcatagagtt
    10981 cttgaagtcc ctggtactgg ggcgttaaac cacatccagt agcagtgttg acaatcaaga
    11041 gaactttgcc acgatagcta tccaagggag ttgcttggtt gttttggttc aaaacggaaa
    11101 aatcataaag tgaagtcatg agggcctctt ttctttttct acattgtaac agtttttact
    11161 tttttcgtcg agtcggatgg aagatattgt tgaagaataa gccctcaata tgatagaaac
    11221 cgtttacaaa taactttaaa aatggtataa tttaattaaa ccggtttact tagttgctat
    11281 aaatctctaa tttgagattt agttcctatt gattttacaa tatgtttatt ggagtgtata
    11341 catgcaaaca aaaacaaaga agctcattgt gagtttgtct tcacttgttt tatcaggatt
    11401 tttattaaac cattatatga cagttggagc ggaagaaacg actacgaata ccattcagca
    11461 aagccagaag gaagttcagt atcagcaaag ggatacaaaa aatttagttg aaaatggtga
    11521 ttttggtcag acggaggacg gaagcagtcc gtggacagga agcaaagctc aggggtggtc
    11581 agcttgggta gaccagaaga atagttctgc agatgcctca actcgagtca ttgaggctaa
    11641 ggatggggct atcactatct caagccctga gaaattaagg gcagcggttc accgtatggt
    11701 tcctattgaa gctaagaaaa agtataaact gcgtttcaag attaaaacag atcataaagt
    11761 cgggattgcc aaagttcgta tcattgagga aagtggtaag gacaagcgat tgtggaattc
    11821 tgcaacgacg tcaggaacaa aggactggca gaccattgaa gcagactata gcccgacttt
    11881 agatgttgat aaaatcaagc tggagttatt ctatgaaaca ggaactggga ctgtttcctt
    11941 taaggatatt gagctggtag aggtagcaga ccagctttct gaggattctc aaacagataa
    12001 acagcttgag gaaaagattg atttaccaat tggaaaaaaa catgtttttt ctcttgcgga
    12061 ctatacttat aaggtagaaa atcctgacgt tgcttcagtc aaaaatggaa ttttagaacc
    12121 tcttaaggaa gggacaacca atgtcattgt cagtaaagat ggcaaggaag tgaaaaagat
    12181 tcctttgaag attctagcct ctgttaagga tgcatacaca gaccgtttgg atgactggaa
    12241 tggcatcatt gctgggaatc aatactatga ttctaaaaat gaacagatgg ccaaattaaa
    12301 ccaggaattg gaaggaaagg tagctgatag cctatctagt atttcaagtc aggcggaccg
    12361 cacctatttg tgggaaaaat tttcaaatta taaaatgtct gcaaatctga ctgccactta
    12421 tcggaaattg gaggagatgg ccaagcaagt gaccaatcct tcttctcgtt attatcaaga
    12481 tgaaactgtc gttcgaacag tcagggattc catggaatgg atgcataaac atgtctacaa
    12541 tagtgaaaag agcattgttg ggaactggtg ggattatgaa atcggtacac ctcgtgccat
    12601 caacaatacc ttgtctctga tgaaagaata cttctctgat gaggaaatta aaaaatatac
    12661 agatgtgatt gaaaaatttg taccagatcc cgaacatttc cgaaagacga ctgataaccc
    12721 attcaaggct ctaggtggaa acttagttga tatgggaagg gtaaaagtaa tagctggttt
    12781 actgcgtaag gatgatcaag aaatttcttc taccattcgc tcgattgagc aagtgttcaa
    12841 gttggtagac caaggtgaag gtttttatca agatggatcc tatatcgacc ataccaatgt
    12901 tgcctatacg ggtgcttatg ggaatgtttt gattgatggc ctgtctcaac tgttgccagt
    12961 cattcaaaag accaagaatc caatcgataa agataaaatg caaaccatgt accactggat
    13021 tgataaatcg tttgctcctt tgctggtgaa tggagagctg atggatatga gtcgtggacg
    13081 ctcgatcagt cgtgcaaata gcgaggggca cgtggccgca gtagaagtac taagagggat
    13141 tcaccgaata gcggatatgt ctgaaggaga aaccaaacaa cgtttgcaga gtcttgtgaa
    13201 gaccattgtt caatcggata gttattatga tgtctttaag aatttgaaga cttataagga
    13261 tatcagtttg atgcaatcct tgttaagtga tgcaggagtc gcaagtgttc caagaacaag
    13321 ttacctatct gcctttaaca agatggataa aacagtcatg tacaatgcag agagagggtt
    13381 tggatttggc ttgtcactct tttccagtcg taccttgaat tacgaacaca tgaacaagga
    13441 aaataaacgt ggttggtata cgagtgatgg gatgttctat ctttacaatg gcgatttgag
    13501 tcactatagc gatggctact ggtcaacagt taatccatat aagatgcctg gtacaacaga
    13561 gacggatgct aagagagcgg atagcgatac aggtaaagtt ctaccgtctg ctttcgttgg
    13621 aacgagcaaa ctagatgatg ccaatgcgac agcaaccatg gatttcacca actggaatca
    13681 aacattgact gctcataaga gctggtttat gctgaaggat aagatcgcct ttttaggaag
    13741 caatatccaa aacacttcaa cagatactgc tgcaactaca attgaccaga gaaaactgga
    13801 atcaagtaat ccatataaag tctatgtcaa tgataaagaa gcctccctta cagaacaaga
    13861 aaaggattat cctgaaaccc aaagtgtctt tttagaatcg tccgattcga aaaagaatat
    13921 tggttacttt ttctttaaga agagttcaat cagtatgagt aaggctttgc aaaagggagc
    13981 ctggaaggat atcaatgaag gacagtcaga caaggaagtt gaaaatgaat ttcttacgat
    14041 tagtcaggct cataagcaaa atggagattc ttatggctat atgctaattc ctaacgtgga
    14101 tcgtgccacc ttcaatcaaa tgataaaaga gttagaaagt agcctcatcg aaaataacga
    14161 aacccttcag tctgtttatg atgctaaaca aggagtttgg ggcattgtga aatatgatga
    14221 ttctgtctct actatttcca accaattcca agttttgaaa cgtggagtct ataccattcg
    14281 aaaagaaggg gatgaatata agattgccta ctataatcct gaaacccagg aatcagctcc
    14341 agatcaggaa gtctttaaaa agctagagca agcagctcag ccacaagtac agaattcaaa
    14401 agaaaaggaa aaatctgaag aggaaaagaa ccattcggat caaaagaatc tccctcagac
    14461 aggagaaggt cagtcaatct tggcaagtct agggttcttg ctacttgggg cattttatct
    14521 attccgtaga ggaaagaaca actaatttgt tcatcatcta gatgaattac atgaaactgt
    14581 ggaaaataga aaaaatccga agaaggccaa atatcttcgg atttttcttt taaaaaagga
    14641 taagttgata cttatccttt tttacttcat tattatgtga ctatagcgtt ttgaaactag
    14701 aatagaacgt tataactgct aaaagatttc tataaattca tttgattttc ctaatcaatt
    14761 tgttcgtatc ctatttcact ccactatata agtccgtctc gctccgtgag gtgcgcgtcg
    14821 aaggttatac caaaaaaact ccaaacgcga tacaataaag gtattcaatc caattgtaaa
    14881 gcgaaaggag aaaaaatatg gcacaaaaga ctcatagttt atcatacaca aagtggatgt
    14941 gttctatcat attgtgttca cccctaagta tagacgaaaa gtcatctata atcaatatag
    15001 aagtagttta ggcgaaatat ttcatcgctt gtgtagttat aaaggtgttg aaatgatcga
    15061 gggtcactta atgccagacc atgtacacat gttagtcagt attccactaa ggctaagtgt
    15121 ttcaagtttt atggggtatt taaaaggcaa aagtgcactc atgatgtttg acaaacacgc
    15181 caatctcaag tacaagtttg ggaatcggca tttctgggcg gaaggttatt atgtaagtac
    15241 agtagggctt aataaagcca caattaagaa atatagtcaa aattaaagaa attatcgagt
    15301 ggatgatttc ttcacgagta tgaaaatttg agaactagta aagtatgata tatagtaaaa
    15361 tgaaataaga acaggacaaa atcgatcagg acagtcaaat cgatttttaa caatgtttta
    15421 gaagtagagg tgtactattc cagtttcaat ctactatagc actagataaa ttgagtgtaa
    15481 aagaatatga ggatcccttt agggatagtg gtaagtaata ccaaagtctc ttaaagaggc
    15541 aagtgacgag tcaagagcaa taaggcttga acaacgtgaa agccagcgtc tttaggcgct
    15601 ggctggtaat ttgggcttat agctctggga taaaccaccc gtttgacggg tggttatgat
    15661 tttatctgag tgtaacatac tgttgggcaa tctcgctgat gcggtcaaag ttgccttggg
    15721 aagcgagttt attgagttcg ccaccaattc caacggcatc tgcaccagca gcgaaccatt
    15781 gagggatgtt gtttagaccg actcctccgg ttaccattac ggaaacttgt ggaatcggtg
    15841 ccttgactgc agagatatat gctggactga gagtactacc tgggaagagt ttgatgattt
    15901 cactaccggc ttcaagtgca gtcgtgatct ctgtgagggt aatacagcct ggaatgtacg
    15961 gtgtgctgta gagattgcac attttcgcag tttcagcatg gaaagatgga gaaacaacgt
    16021 aatttgctcc agctagaatg gcatctctag cagttacggc atcaagcaca gtacctgcac
    16081 cgatacaaac actctgatcg tcctgataca agtctacaag ttccttgatg atttgtcctg
    16141 catactgatt ggtataggcg atttcaatag ctttgatacc gcccttgata caagcaatcg
    16201 aggcttgcag tccttcttcc tttgtatttc ctcgaataac agcgacaatt ttttgttttt
    16261 ttagttcaat aatcgtatct gatttggtca tgtaattctc ctaacgaatg atatcttgtg
    16321 catttgccag taaattttca atactagttg cggaagtgga gagatggtct cctggaagag
    16381 tgcatttgag agttgcgctc gcaactgcaa agtcaatggt agtttttagg gaggaatgat
    16441 ggagtagttg gtaaagggca ccagatataa aggcatcccc gctaccaatt cgttgataga
    16501 ctgcagtttt tagttggaca gactcttcaa atagttcttc tagagcatag gcttgataga
    16561 catttttgtc ttgctcatca ctagagcgga gggtatggaa aatggccttg aaaccatagg
    16621 cttcttttaa aagtcgcatg cgattttcca cctcttctag gctagcactg tctcttggaa
    16681 acatctctag attttggtca tcaatcatga gaggatcaat accgaagcaa tagtcagtaa
    16741 aacgtgcaaa cttagaaaat tcatacttgg cttctaggac tgaaatcatc tttgttctca
    16801 gattgagatc cattgaaacg acaattcctc ggcgcttggc ttcttccaag agtaggagaa
    16861 ggatcgtacg gacctcttga ccgatagcta cggtgattcc actaaaatga aaatggctaa
    16921 tcccctgaaa gagagaatct atatctagca tgtttggccg aatctggctg atactcgtat
    16981 gcttacgatc gtagaaaact tcactttgac gacaaccaaa gccgttctcc aaatagtaga
    17041 ggccgattcg atcgccaagc cgacagattg aactggtatc gatttggtgc tgtttcaaga
    17101 atgtgagaaa acgatctcca atctcgttgg cagggagtgc ggtaaagact ttcgttgaga
    17161 tacccagggc ttgcaaatta caagcgatgt taatttctga tccgccaaaa taagtcgaac
    17221 tggcaacatg atcgccgata ctggtggcat ctaatggtga gattcgaatt aatggttcgc
    17281 caaacagtaa tattttcgtc atccattagt tcctttctgg tttagaatag ctttacttgg
    17341 ctaatacttc tttcaaataa gcagcgactt catcatcttg gcagttttca aagaagtatt
    17401 cttggaagta tttgccagag atggtgtctt tgaggaagtc ttggtcgatt gttttaagga
    17461 tggtcatcaa atcattgtgg gcgatttgtt tcacctcgtt taagatacga gcgttgcgtt
    17521 gttcagggat tacacgttct cttgggtagc caccgcccat ttcttcagca aataagcgtt
    17581 caaacatcaa tttgagggtc agttctgccc cccagccaaa tcctttggca taagggatag
    17641 acaaggcgtt accaccattg atttgagaat aaaggtaagc gtcagttggg tccactgcta
    17701 gaccacagac aacaccaggg aagctgttta aagcaagcat agcccctaca cccgtaccac
    17761 agccggtaac aacaaagtca actgcctttg tatttaaaag gatggcagcc attagtccgt
    17821 tctgcacata agttaattga ctttctcctt cttctccacg cataccatag ttaaataatt
    17881 ggtagccttt tttatctgtc gcttctttta gactatcgta aataatgtga ttcttgctag
    17941 cttgactatt ttcattgatt aatgcgattt tcataagatc ttctttctat ttttactcag
    18001 gttgttttcc gatgtaggct aagataccgc catctacata taggatgtgg ccattgacaa
    18061 aattgctggc atcactagcg agaaagacag cagggcccat caaatcttca ggatttcccc
    18121 aacgtgcagc aggtgttttt gcaatgatga actggtcaaa tgggtggcga gaaccatctt
    18181 cttgcaactc acgaagaggt gctgtttgag gagtggcaat ataacccggt ccaattccgt
    18241 tacattggat attggctcca ccgtattcag acgcaatgtt gcgggtcaac attttcaagc
    18301 cccctttagc agcagcataa gcgctaactg tttcacgtcc cagttcgctc atcatcgaac
    18361 aaatattgat aatctttcca tgccctttct ttatcataga aggaataact gcctttgaaa
    18421 cgataaatgg tgcgtttaag tcaatatcga tgaccttacg gaaatcagcg gcgctcattt
    18481 cgcacattgg aactcggcgg ataataccag cgttattaac gaggatgtca atgacaccaa
    18541 cctcttgttc gatttgcttg accatggctt ggataccgtc ctcgtctgtc acatcacaga
    18601 catatccatg ggcttcgatg ccaacttcac gataagcttc aatcccttta ttgaccagat
    18661 cttgattgat atcgttaaag acaatagtag cgccggcttc agcgtaggat ttggcaatag
    18721 caaatccaat tccataagaa gcgccggtga tgagagcaat ttttcctttt aaagaaaact
    18781 gctcaattga gaatgatgta tttgtcattt gatacttcct ttcaaatttg ttaagacttc
    18841 tctataacca aattatattc tagtaaaccg gttttgtcaa taaataaata tttttattta
    18901 taaaaaggtt tgttttccta aaaaaacgga agattttgaa atttctgcct attaaaatgg
    18961 ttaaaaagat aatgaaagcg ctaatatttt tttaaaaaaa ctattgacaa gataaaaata
    19021 aaggattaca atggggaata taaagtaaac cggtaaacct aaacaagaaa ggagaaaaga
    19081 tgaaaattgt acttgtaggg catggacatt ttgctacagg gatttatagt tctttacaat
    19141 tgattgcagg caatcaagaa aatgtggagg caattgactt tgtggaagga atgtcagcag
    19201 atgaactcaa gcaaaaaatc ttacttgcaa tttcaaatga agaagaagtt ttaatcctaa
    19261 gtgatctctt gggaggatcg ccattcaagg tttcttctac cataatggga gaaaatccag
    19321 ccaaaacaat gaatgttctc tcgggtttga acttagccat gttaatggaa gcagtctttg
    19381 ttagaatggc tcatagcttt gatgaagttg ttaataaatc agtagtggcg gcccagggcg
    19441 gagtcgtaaa tggtaaagaa ttgttttcaa cggatgcaga ggaagaggaa gaagatttcg
    19501 aatcgggtat ttaaagggta aaagaatgat aaaaaaggtt acgattgaaa aaataaaatc
    19561 gcctgagcgc ttcttagaag taccacttct gacgaaagaa gaagtcggcc aggcaatcga
    19621 taaggttatt cggcagttag aactcaacct tgactatttc aaggaagatt tcccgacacc
    19681 agctaccttt gataatgtct atccaatcat ggataacacg gaatggacca atggtttctg
    19741 gacaggagaa ctgtggttgg cttatgaata cagtcaacag gatgcattta aaaacatcgc
    19801 tcataaaaat gttctttctt tcctggatcg tgtcaataag agagtagaat tggatcacca
    19861 tgatctcggc ttcttgtaca caccgtcttg tatggctgaa tataagataa atggagatgg
    19921 agaggctaga gaagcaacct tgaaagctgc agataagttg attgaacgct atcaagaaaa
    19981 aggtggtttt attcaagctt ggggagactt gggcaagaaa gagcattacc gtttgattat
    20041 cgactgcttg ctcaatatcc aactcttatt ctttgcttat caagaaacag gcgatcaaaa
    20101 atactacgat attgcagaaa gccatttcta tgcttcagct aataatgtaa tccgtgatga
    20161 cgcttcgtcc ttccacacct tctattttga tcctgagaca ggtcaaccct ttaaaggtgt
    20221 aacgagacaa gggtatagtg atgattcatg ctgggcacgt ggtcagtcat ggggagtcta
    20281 tggtattcct ttgacttatc gtcacttaaa agacgagtcc tgctttgact tgtttaaggg
    20341 tgtgaccaat tatttcttga atcgtctgcc aaaagatcat gtgtcctatt gggatttgat
    20401 ttttaatgat ggtagtgatc aatcacgaga ttcttcagca acagctatcg ccgtctgtgg
    20461 gattcatgaa atgctaaaac atctcccaga ggtggatgct gacaaagata tttataaaca
    20521 tgctatgcat gccatgcttc gttccttgat cgaacattat gcaaatgatc aatttacccc
    20581 tggtgggaca agtctcctcc acggtgtgta ctcatggcat tcaggtaaag gagtggatga
    20641 aggcaatatc tggggtgact actattacct agaagccctt atccgtttct acaaagactg
    20701 gaacctatat tggtaggagg agaaatatga caatgccaaa tattattatg acccgtatcg
    20761 atgaacggtt gattcatggg caaggacaac tttgggtaaa atacctaggt tgtaatacgg
    20821 tcattgttgc caatgacgaa gtaagcacag acaagatgca acaaactctg atgaaaacag
    20881 ttgtgccaga ctcagttgcc atgcgtttct tccctttgca aaaggtgatt gatatcattc
    20941 acaaggctaa tcctgctcaa acgatcttta tcgttgtaaa ggatgtgaag gacgctttaa
    21001 ccttggtaga aggtggtgtc cctatcaaag aaatcaatat tgggaacatt cacaatgcta
    21061 ctggtaaaga gcaagtgaca cgctccatct tcctgggtga agaggacaag gcggccctca
    21121 aggaattgag ccaaactcat caagtaacat ttaatacgaa aacaactcca acaggaaatg
    21181 atggagctgt tcaagtcaac attatggact atatttaaaa gaggagatcg ttatgtcgat
    21241 taatgtattt caagcgattt taattggatt atggacagct ttctgtttta gtggaatgct
    21301 gttaggaatt tacaccaata gatgtattgt tctgtcattt ggtgtcggaa ttattctagg
    21361 tgatctgcct actgctcttg caatgggagc tattggtgaa ttggcttata tgggattcgg
    21421 tgttggtgct ggaggtactg ttccaccaaa cccaatcgga cctggtatct ttggtacctt
    21481 gatggctatc actagtgctg gtaaagtcag tccagaagcg gctcttgccc tctctactcc
    21541 gattgctgtg gcgattcaat tcttacaaac tttcgcctac actgtacgtg ctggtgcgcc
    21601 tgaaacggcg atgaagcact tgaaaaacca taatttgaag aaatttaagt ttactctaaa
    21661 tgcaacaatt tggttgtttg cctttattgg atttaccttg ggttgcttgg gtgccctttc
    21721 aatggatacc ttgttgaaac tcgtagacta cattccaccg gtattgctta caggtttgac
    21781 agttgctggt aaaatgctcc cagctatcgg ttttgcgatg atcttgtcag tgatggctaa
    21841 gaaagagttg attccctttg tcttgttggg atatgtttgt gcagcttatc taaacatccc
    21901 aacaattggt attgcaattg taggtactat ctttgctttg attgaatttt ataacaagcc
    21961 aaaaacagcg gatcatgtgg tagaggagga agcacacgat gactggatct aacaaattaa
    22021 caaaacgtga ttatcttaaa acgtctttgc gggcattctt ttgtcaaaat ggatttaact
    22081 atagtaacta tcaagggttg ggatatgcca atgtgatgta tcctgctttg aaaaaacact
    22141 atggagagga tcaggaaggt ttctaccaag ccttggaaga aaactgtgaa ttctataata
    22201 ccaacccaca cttcctgcct tttattacca gcttgcatct tgtaatgttg gaaaatggcc
    22261 gaccaacaaa agaaacacgt agcatcaaga tggccttgat gggaccattg gcaggtattg
    22321 gggattctct ttctcaattc tgtttagctc cattgttctc aaccatcgca gcttcgtttg
    22381 ctcaagaagg cttggttgtc ggtccaatct tgttcttcct tgcgatgaat acgattttaa
    22441 cagcgattaa attgtcaact ggtctgtatg gatacaaact aggaacaact gtgattgata
    22501 aactaagcga acagatggca acgatttctc gtattgccaa tattatcggt gtaaccgtaa
    22561 ttgctggttt ggcagcgaca tctgttaaga ttatggtgcc gattaccttt gctgcagggg
    22621 aagttaaagc agacgctaaa caaagtatcg taagtattca gggaatgctt gataaggttg
    22681 ctccagctct tctaccagcc ctatttacac ttttagttta ctacttgatc aaagaaaaga
    22741 aatggacaac atataaactc gttattttaa cagttatcat cggaattatc ggaagctggc
    22801 ttaagattat agcttaatgg atacaacatt gttttatgga atagtgattg tcttggcagt
    22861 gagtcccctt ttactgtcaa gctttcattc tattcgtcag caaaagttgc ttcgcaaaca
    22921 gatggagcaa cgacaagagt atttagcttc tttaacatct ggtgatgaag tgttgttatt
    22981 gtctggaatt catggaaaaa tcatttctat caaagatgac ttggtctcct tgcagattgc
    23041 aaaaggtgtg gtcatctatg tagaaaagga aagtgtaatg ggaaagacaa aagaactgct
    23101 ttttaagtag ttctgtttct ggtaatagat aatgaaagaa gaaagaagac aattttttga
    23161 aagagtcgat ggaaaccaat gtcgtgatta tatcttgtcc cactgttcaa aagactatga
    23221 gaaagtcaag agttcccttg aacgcttgat ggacaatcgt tttatgtttg atagtccttg
    23281 ggatatggag ccttgttcaa aaatccatca aatccagccg atggtatggg atcaagtatt
    23341 tgaagatgat ccagaatggt cttatatgct caatcgacaa gaatatctct tgcagtttat
    23401 gatagggtat ctggtagaag gagataagga ctatattcaa aagtgcaagt tctttctatt
    23461 tgattggatt gagcaggtga gagaattttc tcctcaatcc ttgatgacta gaaccttgga
    23521 tacgggtatt cgttccttta cttggttgaa actactcttg ctcctcttga aatttgactt
    23581 gctagaggag aaagaactag agaaaatttt ggtcagtcta gaaaagcaga ttgactttat
    23641 gaaaagctac tatcgcgcca agtacaccct tagtaactgg gggattttac aaacaattcc
    23701 gatgcttgct atctatcatt tcttttcaga taagatggat ctagaagaag cttaccattt
    23761 tgcttcagag gagttgaaac agcaaattga gacacagatt ttaggagatg gaagccagtt
    23821 tgaacagtcg attctctatc atgtagaagt ttataaagcc ttgctggatt tgtgtctctt
    23881 gcttccagac ttgcaagata gttaccaaga gttgctggaa aagatggcga cctatattca
    23941 aatgatgaca ggcttagatg gacggacttt ggcttttggt gatagcgatt ctacagaaac
    24001 gacagaaatg ttgagcctgt ctgctgtggt tttgaacaag gaagaccttc ttaacggtct
    24061 ggatgttaaa gttgatttgc ttagcctctt gttcctaggg cgagaaaagg tcaagcgact
    24121 gcaggaattt gaaaggagag cttggcagcc taagtccatg atctttgaag actctggaca
    24181 tgtctgcatt aaggatgaac atcgttatct atttttcaaa aatggtccgc taggaagtgc
    24241 ccatagccat agtgacgaga atagtttttg cttacagtat caaggccaac ctattttcat
    24301 agatgctggg cgttattctt atcgggagat atatgaacgt tatctcttaa agagtgcttg
    24361 gagtcattcg acctgcattg tagatgggaa agctccggaa agaatcacag gatcctggga
    24421 atatgaatac tatcctcact ccctgttttg tcaccataaa gaaagggagg gaatgcatta
    24481 tattgaaggg gcttattggt cagcagaacc tgatttgcct tatcttcaca gaagaaaaat
    24541 cctcatgttg gtagaggatg tctggctctt ggtggatgac atcaggtgtc aaggtcagca
    24601 tgaggcgttg actcagttta tccttgacaa ggatgtgacc tatcaagatg ggaaaatcaa
    24661 tcagttgaga ctatggagtg aagttgattt tgatttggaa gataccatca tttctcctaa
    24721 atacaatgag cttgaaagaa gtagcaaact caccaagcgc caattctttg agaatcagat
    24781 gctggattat accatcattg cgcatgagag ttttgaaatc atccgtcatt ctgtctacca
    24841 gacagatgat cgtgaagtgg aaaatgctct ggcttttgaa gtgaaaaatg acgaaacaga
    24901 caagctgatt ctgttattaa gcgaggatat tggtgtaggt gaaaaattgt gcctcgttga
    24961 cggaacaaaa atgcgtggaa aatgtctagt atatgataaa ataaatgaga gaatgattcg
    25021 cttgcagtgc tagaaatagg cattttgaat agtgaatatg ttataataag tattagtagg
    25081 aggtgtttta gattggagaa gaaactgacc ataaaagaca ttgcggaaat ggctcagacc
    25141 tcgaaaacaa ccgtgtcatt ttacctaaac gggaaatatg aaaaaatgtc ccaagagaca
    25201 cgtgaaaaga ttgaaaaagt tattcatgaa acaaattaca aaccgagcat tgttgcgcgt
    25261 agcttaaact ccaaacgaac aaaattaatc ggtgttttga tcggtgatat taccaacagt
    25321 ttctcaaacc aaattgttaa gggaattgag gatatcgcca gccagaatgg ctaccaggta
    25381 atgataggaa atagtaatta cagccaagag agtgaggacc ggtatattga aagcatgctt
    25441 ctcttgggag tagacggctt tattattcag ccgacctcta atttccgaaa atattctcgt
    25501 atcatcgatg agaaaaagaa gaaaatggtc ttttttgata gtcagctcta tgaacaccgg
    25561 actagctggg ttaaaaccaa taactatgat gccgtttatg acatgaccca gtcctgtatc
    25621 gaaaaaggtt atgaacattt tctcttgatt acagcggata cgagtcgttt gagtactcgg
    25681 attgagcggg caagtggttt tgtggatgct ttaacagatg ctaatatgcg tcacgccagt
    25741 ctaaccattg aagataagca tacgaatttg gaacaaatta aggaattttt acaaaaagaa
    25801 atcgatcccg atgaaaaaac tctggtattt atccctaact gttgggccct acctctagtc
    25861 tttaccgtta tcaaagagtt gaattataac ttgccacaag ttgggttgat tggttttgac
    25921 aatacggagt ggacttgctt ttcttctcca agtgtttcga cgctggttca gccctccttt
    25981 gaggaaggac aacaggctac aaagattttg attgatcaga ttgaaggccg caatcaagaa
    26041 gaaaggcaac aagtcttgga ttgtagtgtg aattggaaag agtcgacttt ctaaaatgaa
    26101 ggaaaatgac ttgcaatttc tgttaagaaa taaaataatc ccacctagaa caagctaggt
    26161 gggattattt gcctatgaaa tgagaaatta tgggagcaag ctcctaaatc aactgttttt
    26221 gatctacttc tttaactact tgataaaagt tatagaagta ggccaaactt gaaatgatgg
    26281 ttacgactag gaatattgaa aatttccatt ggacagggtt ggttaaaagt tgtggaaagg
    26341 atatgaggag aaagaagagg gctgcgttga ggacaggtat ccgttttgat tgtattttct
    26401 caagtccttt attgagcgca ggaagaaaga ggagtaggag tagtaaaact gtatgagaaa
    26461 cagctcctga aataagggcg aagaaaagga aaatactgat aaaaacatga atgatcagta
    26521 gtctagctag tgatttcata aggcacctcc taatcctggt cttttttagc tcttgcaata
    26581 cgaagtgagt cgacaatatg tatcatcact ccgaaaaaga aagctcccag tatagtttta
    26641 aaaatatgtt ttgtatttag aagagaactg ataaaatttg gattttcact tgttagggta
    26701 tcaatgagtg gaattataaa aaatatcact gttccataaa tcgaacctgc tttcagacca
    26761 ggataacgta actgtttctt ttcttttttc atgagtttgc tcctttttta aaaatcttcc
    26821 ttccaaaaga gactgttgag gtcagtttgg aggctgcggg cgagattgag acagagttcc
    26881 agagttggat tgtacttgtc gttttcaatc atattgatgg tctgtctcga gacaccgata
    26941 tccttggcga gctcgagctg ggaaatgccc agttccttgc gaaattcttt cacacgattc
    27001 atctggtctc ctttctgatt tgtgtcgtat atatttgact ttattatagt cttttaaaca
    27061 taaagtgtca agtatttttg acatattttt tgaagaaata gtagtctcct tgtcctattt
    27121 gtctgacaag tgcaagctgg tcggatttgt ggtaaaatag ataagatatg acaaaagaat
    27181 ttcatcatgt aacggtctta ctccacgaaa cgattgatat gcttgacgta aaacctgacg
    27241 gtatctacgt tgatgcgact ttgggtggag caggccatag cgagtattta ttaagtaaat
    27301 taagtgaaaa aggccatctc tatgcctttg accaggatca gaatgccatt gacaatgcgc
    27361 aaaaacgctt ggcaccttac attgagaagg gaatggtgac ctttatcaag gacaacttcc
    27421 gtcatttaca ggcacgtttg cgcgaagctg gtgttcagga aattgatgga atttgttatg
    27481 acttgggagt gtctagtcct caattagacc agcgtgagcg tggtttttct tataaaaagg
    27541 atgcgccact ggacatgcgg atgaatcagg atgctagtct gacagcctat gaagtggtta
    27601 atcattatga ctatcatgat ttggttcgta ttttcttcaa atacggtgag gataaattct
    27661 ctaaacagat tgcgcgtaag attgagcaag cgcgtgaagt gaagccgatt gagacaacga
    27721 ctgagttagc agagattatc aagttggtca aacctgccaa ggaactcaag aagaaggggc
    27781 atcctgctaa gcagattttc caggctattc gaattgaagt caatgatgaa ctgggagcgg
    27841 cagatgagtc catccagcag gctatggata tgttggctct ggatggtaga atttcagtga
    27901 ttacctttca ttccttagaa gaccgcttga ccaagcaatt gttcaaggaa gcttcaacag
    27961 ttgaagttcc aaaaggcttg cctttcatcc cagatgatct caagcccaag atggaattgg
    28021 tgtcccgtaa gccaatcttg ccaagtgcgg aagagttaga agccaataac cgctcgcact
    28081 cagccaagtt gcgcgtggcc agaaaaattc acaagtaaga gggaaaaaga tggcagaaaa
    28141 aatggaaaaa acaggtcaaa tactacagat gcaacttaaa cggttttcgc gtgtggaaaa
    28201 agctttttac ttttccattg ctgtaaccac tcttattgta gccattagta ttatttttat
    28261 gcagaccaag ctcttgcaag tgcagaatga tttgacaaaa atcaatgcgc agatagagga
    28321 aaagaaaacc gaattggacg atgccaagca agaggtcaat gaactattac gtgcagaacg
    28381 tttgaaagaa attgccaatt cacacgattt gcaattaaac aatgaaaata ttagaatagc
    28441 ggagtaagat atgaagtgga caaaaagagt aatccgttat gcgaccaaaa atcggaaatc
    28501 gccggctgaa aacagacgca gagttggaaa aagtctgagt ttattatctg tctttgtttt
    28561 tgccattttt ttagtcaatt ttgcggtcat tattgggaca ggcactcgct ttggaacaga
    28621 tttagcgaag gaagctaaga aggttcatca aaccacccgt acagttcctg ccaaacgtgg
    28681 gactatttat gaccgaaatg gagtcccgat tgctgaggat gcaacctcct ataatgtcta
    28741 tgcggttatt gatgagaact ataagtcagc aacgggtaag attctttacg tagaaaaaac
    28801 acaatttaac aaggttgcag aggtctttca taagtatctg gacatggaag aatcctatgt
    28861 aagagagcaa ctctcgcaac ctaatctcaa gcaagtttcc tttggagcaa agggaaatgg
    28921 gattacctat gccaatatga tgtctatcaa aaaagaattg gaagctgcag aggtcaaggg
    28981 gattgatttt acaaccagtc ccaatcgtag ttacccaaac ggacaatttg cttctagttt
    29041 tatcggtcta gctcagctcc atgaaaatga agatggaagc aagagcttgc tgggaacctc
    29101 tggaatggag agttccttga acagtattct tgcagggaca gacggcatta ttacctatga
    29161 aaaggatcgt ctgggtaata ttgtacccgg aacagaacaa gtttcccaac gaacgatgga
    29221 cggtaaggat gtttatacaa ccatttccag ccccctccag tcctttatgg aaacccagat
    29281 ggatgctttt caagagaagg taaaaggaaa gtacatgaca gcgactttgg tcagtgctaa
    29341 aacaggggaa attctggcaa caacgcaacg accgaccttt gatgcagata caaaagaagg
    29401 cattacagag gactttgttt ggcgtgatat cctttaccaa agtaactatg agccaggttc
    29461 cactatgaaa gtgatgatgt tggctgctgc tattgataat aatacctttc caggaggaga
    29521 agtctttaat agtagtgagt taaaaattgc agatgccacg attcgagatt gggacgttaa
    29581 tgaaggattg actggtggca gaatgatgac tttttctcaa ggttttgcac actcaagtaa
    29641 cgttgggatg actctccttg agcaaaagat gggagatgct acctggcttg attatcttaa
    29701 tcgttttaaa tttggagttc cgacccgttt cggtttgacg gatgagtatg ctggtcagct
    29761 tcctgcggat aatattgtca acattgcgca aagctcattt ggacaaggga tttcagtgac
    29821 ccagacacaa atgattcgtg cctttacagc tattgctaat gacggtgtca tgctggagcc
    29881 taaatttatt agtgccattt atgatccaaa tgatcaaact gctcggaaat ctcaaaaaga
    29941 aattgtggga aatcctgttt ctaaagatgc agctagtcta actcggacta acatggtttt
    30001 ggtagggacg gatccggttt atggaaccat gtataaccac agcacaggca agccaactgt
    30061 aactgttcct gggcaaaatg tagccctcaa gtctggtacg gctcagattg ctgacgagaa
    30121 aaatggtggt tatctagtcg gggtaaccga ctatattttc tcggctgtat cgatgagtcc
    30181 ggctgaaaat cctgatttta tcttgtatgt gacggtccaa caacctgaac attattcagg
    30241 tattcagttg ggagaatttg ccaatcctat cttggagcgg gcttcagcta tgaaagactc
    30301 tctcaatctt caaacaacag ctaaagcttt ggagcaagta agtcaacaaa gtccttatcc
    30361 tatgcctagt gtcaaggata tttcacctgg tgatttagca gaagaattgc gtcgcaatct
    30421 tgtacaaccc atcgttgtgg gaacaggaac gaagattaaa aacagttctg ctgaagaagg
    30481 gaagaatctt gccccgaacc agcaagtcct tatcttatct gataaagcag aggaagttcc
    30541 agatatgtat ggttggacaa aggagactgc tgagaccttt gctaagtggc tcaatataga
    30601 acttgaattt caaggttcgg gctctactgt gcagaagcaa gatgttcgtg ctaacacagc
    30661 tatcaaggac attaaaaaaa ttacattaac tttaggagac taatatgttt atttccatca
    30721 gtgctggaat tgtgacattt ttactaactt tagtaggaat tccggccttt atccaatttt
    30781 atagaaaggc gcaaattaca ggccagcaga tgcatgaaga tgtcaaacag catcaggcaa
    30841 aagctgggac tcctacaatg ggaggtttgg ttttcttgat tacttctgtt ttggttgctt
    30901 tctttttcgc cctatttagt agccaattca gcaataatgt gggaatgatt ttgttcatct
    30961 tggtcttgta tggcttggtc ggatttttag atgactttct caaggtcttt cgtaaaatca
    31021 atgaggggct taatcctaag caaaaattag ctcttcagct tctaggtgga attatcttct
    31081 atcttttcta tgagcgtggg ggcgatatcc tgtctgtctt tggttatcca gttcatttgg
    31141 gatttttcta tattttcttc gctcttttct ggctagtcgg tttttcaaac gcagtaaact
    31201 tgacagacgg tgttgacggt ttagctagta tttccgttgt gattagtttg tctgcctatg
    31261 gagttattgc ctatgtgcaa ggtcagatgg atattcttct agtgattctt gccatgattg
    31321 gtggtttgct cggtttcttc atctttaacc ataagcctgc caaggtcttt atgggtgatg
    31381 tgggaagttt ggccctaggt gggatgctgg cagctatctc tatggctctc caccaggaat
    31441 ggactctctt gattatcgga attgtgtatg tttttgaaac aacttctgtt atgatgcaag
    31501 tcagttattt caaactgaca ggtggtaaac gtattttccg tatgacgcct gtacatcacc
    31561 attttgagct tgggggattg tctggtaaag gaaatccttg gagcgagtgg aaggttgact
    31621 tcttcttttg gggagtggga cttctagcaa gtcttctgac cctcgcaatt ttatatttga
    31681 tgtaagaatg gcaccctgat gtttcagggt gtttttgtgt ttaaatacac aatgaaaatc
    31741 aaagaacaaa ctagaaagct aactttaggc tgctcaaaat atagtagatt gaaactagaa
    31801 tagtacacat ctacttctaa aacattttta gaaatcgatt tgactgtcct gaacgatttg
    31861 gcctgttctt atttcatttc actatacaga gttttgagat ggttgataga actgacgaag
    31921 tcagctcaaa acacggtttt gaggttgtag acaaggcgaa gctgatgtgg tttgaagaga
    31981 ttttcgaaga gtgtaaaaaa tattggtcag ttaaagtcaa aatccttgac cttttctgac
    32041 caatgtagta tattatgaac gtaaggtaaa tgaaagcctt actagaacac ttatttaaag
    32101 taaaatagaa agaggtgggt ctatgtttca tctagaaatc ttcagaagta aagatggtct
    32161 actcctgctt gaaaaagaaa aaccagaaat agtacgtagg gtagtggttt agctagtcta
    32221 aattttttaa aacaatggtc aaagatagtc aatatcagta atcataacta agtaaacaaa
    32281 aagaggtaaa gaatatgaat aacaacttta ataattttaa taacatggat gatttattta
    32341 accaattgat gggtggtatg cgaggatata gttctgaaaa tcgccgttac ttaattaatg
    32401 gacgcgaagt cacacctgag gaatttgctc actatcgtgc gactggtcaa ttaccaggaa
    32461 atgcagaaac tgatgtgcaa atgccacaac aggcatcagg tatgaaacaa gacggtgtcc
    32521 ttgcaaaact aggtcgaaac ttgacagcag aagcgcgtga gggtaagttg gatcctgtta
    32581 tcggacgaaa caaggaaatt caagaaacat ctgaaatcct ctcacgccgc accaagaaca
    32641 atcctgtttt ggtcggagat gcaggtgttg gtaagacagc agttgtcgaa gatctagcgc
    32701 aagccattgt gaacggagat gttcctgctg ctatcaagaa caaggaaatt atttctattg
    32761 atatctcagg tcttgaggct ggtactcaat accgtggtag ctttgaagaa aatgtccaaa
    32821 acttagtcaa tgaagtgaaa gaagcaggga atattatcct cttctttgat gaaattcacc
    32881 aaattcttgg tgctggtagc actggtggag acagtggttc taaagggctt gcggatattc
    32941 tcaagccagc tctctctcgt ggagaattga cagtgattgg ggcaacaact caagacgaat
    33001 accgtaacac catcttgaag aatgctgctc ttgctcgtcg tttcaacgaa gtgaaggtca
    33061 atgctccttc agcagagaat acttttaaaa ttcttcaagg cattcgtgac ctctatcaac
    33121 aacaccacaa tgtcatcttg ccagacgaag tcttgaaagc agcggtggat tattctgttc
    33181 aatacattcc tcaacgtagc ttgccagata aggctattga ccttgtcgat gtaacggctg
    33241 ctcacttggc ggctcaacat ccagtaacag atgtgcatgc tgttgaacga gaaatcgaaa
    33301 cggaaaaaga caagcaagaa aaagcagttg aagcagaaga ttttgaagca gctctaaact
    33361 ataaaacacg cattgcagaa ttggaaaaga aaatcgaaaa ccacacagaa gatatgaaag
    33421 tgactgcaag tgtcaacgat gtggctgaat ctgtggaacg aatgacaggt atcccagtat
    33481 cgcaaatggg agcttcagat atcgaacgtt tgaaagatat ggctcatcgc ttgcaagaca
    33541 aggtgattgg tcaagataag gccgtagaag ctgtagctcg cgctatccgt cgtaaccgtg
    33601 ctggttttga tgaaggaaat cgcccaatcg gcagcttcct ctttgtaggg tctactgggg
    33661 ttggtaagac ggagcttgct aagcaattgg cactcgatat gtttggaacc caggatgcga
    33721 ttatccgttt ggatatgtct gaatacagtg accgcacagc tgtttctaag ctaattggta
    33781 caacagcagg ctatgtgggt tatgatgaca atagcaatac cttaacagaa cgtgttcgtc
    33841 gcaatccata ctctatcatt ctcttggatg aaattgaaaa ggctgatcct caagttatta
    33901 cccttctcct ccaagttcta gatgatggtc gtttgacaga tggtcaagga aatacagtaa
    33961 acttcaagaa cactgtcatt attgcgacct caaatgctgg atttggctat gaagccaact
    34021 tgacagaaga tgcggataaa ccagaattga tggaccgttt gaaacccttc ttccgtccag
    34081 aattcctcaa ccgctttaat gcagtcatcg agttctcaca attgactaag gaagaccttt
    34141 ctaagattgt agatttgatg ttggctgaag ttaaccaaac cttggctaag aaagacattg
    34201 acttggtagt cagtcaagcg gctaaagatt atatcacaga agaaggttac gacgaagtca
    34261 tgggggttcg tcctctccgt cgcgtggttg aacaagaaat tcgtgataag gtgacagact
    34321 tccacttgga tcatttagat actaaacatc tggaagcaga tatggaagat ggcgttttgg
    34381 ttattcgtga gaaagcctaa gacagaattt tgaggataaa aaagaaggag ccagctgaaa
    34441 aaactggttc cttttgaggt acgacgggca tgtcgtatag taggttgaaa ctagaatagt
    34501 acacatctac ttctaaaaca tttttagaaa tcgatttgac tgtcctgatc gatttgtcct
    34561 gttcttattt cattttacta tatatctgag gtgtaagtcc tcggtgggca cctgttaccg
    34621 gtgaacccaa tagcgattcc caagcctgac tatcgtgagg tagcggatta aaacggtctg
    34681 gggatagacc gttttaagtc tgacgctgga aataagaatc gtcagaggaa gggatagcga
    34741 actcgtggct ctacgaacag gaacgtgata gtaaggcgta tatagcggat aagggggcct
    34801 caaactctaa agtccaaaaa ggtagtcgta acctatatgc gtaaatcacg agagcaactg
    34861 aatttgtcca ttcgaggatg gataaactat ttttcattgg gaaatatgaa aaatatagtc
    34921 actagcatag atgagcgctt gcgtactcgc ctactcgcct acgagtgatt atctggaagc
    34981 aatggaagaa gaaacctaga cgattatgga gattgcttaa gttaggggtt cctaaatgga
    35041 tagcagataa ggtatctggc tggggtgacc actatcaatt agtatctcag aagtcggtac
    35101 ttaaacgtgc tatatcaaaa ccagtccttg agaaacgtgg actggtttcg tgtttggatt
    35161 attaccttga acgacatacg ttaaaagtta gttgaaccgc cgtatgccga acggcacgta
    35221 cggtggtgtg agaggggcta gagattatcc cctactcgat tttaaatcac atgacgttca
    35281 aaggcatcat ctgaaatccc ttgttccaag atgagttttg cccattcttt agcagagaag
    35341 aggctgtggt ccttgtagtt tccgcaagat tcgatggttg tccctgggac atcttcccaa
    35401 gtagtagttt cagcgatttc cttgagcgaa tccttgataa cagctgcgat tttagcactg
    35461 gtgtgacgtc cccacataat catgtggaag cctgtgcggc aaccaaatgg tgaacaatca
    35521 atcatgccgt caatgcgggt acggatgagt ttggctaaga ggtgctcgat agtgtgaagg
    35581 ccggcagtag ggatagagtc ttcgtttggt tgcaccaagc gaatatcata attggagatg
    35641 atgtctcctt ttggtcctgt ttcttcccca atcaagcgaa catagggtgc tttgacaatg
    35701 gtgtggtcaa gttcaaaact ttcgacaata acttcttttg acatggtaaa tcctttcagt
    35761 tttcttctct cattatatca taaaggttgt tcctgagaca gagagaaaac ctctccgagg
    35821 ctagagaggt tgaaatcttt acttacgata taagcggtcg tattggtagt atgggtcaaa
    35881 ggttacgttg atacccagtt tacgaaggac attcttgtct tcatcagtca agatgatggt
    35941 tgagtgggct tcgcttcctt tgaggttgcc gagttcttcc atagcgcggg cagcatcagg
    36001 attttctgta gctgtgatag caagtgcaat caggatttca tttgaatgaa ggcgtggatt
    36061 gcggctaccg agatgatcga ttttaagacc ttggattggc ttaacaactt caggctcgat
    36121 tagttttact tctttagcga tgtcagctga ttttttgatg gcgttgatca aggcagcggc
    36181 tgtaggccca aagagttctg agttcttacc agtgacgatt tccccatttg gcaattcaaa
    36241 ggctagggct ggtccaccag tttcttctgc tttttggcgc gcaacgacag caaccttacg
    36301 gtctgcaggt gtgataccga ggtcgttcat gagcaactca attttcttga cggcagcttc
    36361 gccaactttt tcagctttga agtcaagaac tgtttgatag taacggcgga tgatttcttg
    36421 tttagaagct tcgacagcgg cctcgtcatc tgtaatagcg aaaccaacca tgttgacacc
    36481 catatctgtc ggtgaagcgt atggtgattt tccgagaata cgttccaaca tgcgtttgag
    36541 cactgggaag atttcgatat cacggttgta gttgacagtg gtttctccat aggtttgaag
    36601 atggaagggg tcaatcatgt tgacatcatc aaggtcagct gtggcagctt cataagccaa
    36661 gttaactgga tgatgaaggg gaagattcca aacagggaag gtttcaaatt tagcgtagcc
    36721 agacttgatg ccattgattt ggtcgtggta catattggac atacacgttg ccaattttcc
    36781 agaaccgggt ccaggagcgg ttacgacaat caagttgcga ctggttttga tgtagtcgtt
    36841 tttgcccatg ccttctgggg aaatgatgtg atccatatcc gtcggatatc ctttgattgg
    36901 ataatgaaga taagaatcaa ttccgttttt ctcaagttgg ttgaggaagg catctgcagc
    36961 gggttggcca gcgtattgtg taatgacaac ggaaccaaca aaaatcccta attcattgaa
    37021 tttgtcaatc aaacgaagaa cttcttggtc ataagaaatg cccaagtcgc cacgtgcttt
    37081 ggaatgttca atgttgctag cattaatggc aatcacaacc tcaacctgct ctttcaattc
    37141 ttgcaagagc ttgattttgt tgtcaggttc ataaccagga aggacacgag cagcgtggaa
    37201 atcttctaac attttaccgc caaactctaa gtagagcttg ccgtcaaatt ggttaatgcg
    37261 ctccaaaata tggtcgcgtt gtaaattcaa atactgttca gaactaaaag cttgtttttt
    37321 cattttttta cctctggact ctattataat aaaaaattgg aagttaggaa actacggagc
    37381 taaaaaagaa attaaaaaga ttaagcaaac gcttgcacaa aattttaaaa agtgctatca
    37441 tagactatag attatgaaaa taatgaggta aacagatgca agaaaaatgg tggcataatg
    37501 ccgtagtcta tcaagtctat ccaaagagtt ttatggatag taatggagat ggaattggcg
    37561 atttgccagg aattaccagt aagttggact atctagccaa gctaggaatc acagcaattt
    37621 ggctttctcc cgtttatgac agccctatgg atgataatgg ctacgatatt gctgattatc
    37681 aagcgattgc aacgattttt ggaaccatgg aggatatgga tcaactgatt gcagaagcta
    37741 agaagcgtga tatccgtatc atcatggact tggtggtcaa tcatacctcg gatgagcatg
    37801 cctggtttgt agaggcctgt gaaaatccta atagccctga gcgagactac tatatctggc
    37861 gcgatgaacc caatgaccta gattctatct ttagtgggtc tgcttgggaa tacgatgaaa
    37921 agtcaggtca atactatctc cactttttca gcaagaaaca gccggatctc aactgggaaa
    37981 atgaaaaact tcgccagaaa atttatgaga tgatgaactt ctggattgat aagggtattg
    38041 gtggtttccg tatggatgtt attgacatga ttggcaaaat tcctgacgag aaggtagtca
    38101 ataatggtcc tatgctccat ccctatctca aggaaatgaa tcaggcgacc tttggagata
    38161 aggatctctt gacagtaggg gagacttggg gagcaacgcc agagattgcc aaactctact
    38221 ctgatccaaa ggggcaagaa ttgtctatgg tcttccagtt tgaacatatc ggtcttcagt
    38281 atcaggaagg tcagcctaaa tggcactatc aaaaagagct gaatatcgct aagttaaaag
    38341 aaatcttcaa caaatggcag acagagttag gagttgagga cggctggaat tccctcttct
    38401 ggaacaacca tgacctccct cgtattgtct caatctgggg aaatgaccaa gaataccgcg
    38461 aaaaatctgc caaagccttt gccatcttgc ttcatctcat gagaggaact ccttatatct
    38521 accaaggtga ggaaattggc atgaccaact atccctttga aacgctggat caagtagaag
    38581 atattgaatc tctcaactat gcgcgtgagg ctcttgaaaa aggtgttccg attgaagaaa
    38641 tcatggacag tatccgtgtt attggacgtg acaatgcccg tacccctatg caatgggacg
    38701 agaacaaaaa cgctggtttc tcaacaggtc aaccttggtt ggcggttaat ctaaattacg
    38761 agatgatcaa tgtccaagaa gcgctggcaa atccagattc tattttctat acctatcaga
    38821 aactggtcca aattcgcaag gagaatagct ggctaattcg agctgacttt gaattgcttg
    38881 atacggttga taaggtcttt gcttatatac gtaaggatgg cgaccgtcgc ttcctagttg
    38941 tggctaactt gtccaatgaa gagcaagact tgacagtaga aggaaaagtc aaatctgtct
    39001 tgattgaaaa caccctagct caagaagtct ttgaaaaaca aatcttagtt ccatgggatg
    39061 ctttctgtgt ggaattacta taaatatttt ttgcagaaaa atttaaaatt gaaatcatat
    39121 aaaaacaagg gatgattgta taaaaggtag aaatcctttg ttttttataa ccaaggttta
    39181 taaactttca ttctcgaaat tcaattaact ttacaaattc ccactattaa ggagaaagaa
    39241 gatgaacata aagaagcgtg tccttagtgc aggcctgact tttgcatctg ctttgctttt
    39301 agctgcttgc ggctaatcag gttcagatac aaaaacttac tcatcaacct ttagtggaaa
    39361 tccaactaca tttaactatc tattagacta ttacgctgat aatacagtca attgaaacaa
    39421 gaacaagaca aaagaacctc gtaaaaggta ttgtaacttg gtaatacctt tttgaggtgc
    39481 tttttgatat gagcccatgt tttctcaata ggattgtact caggtgagta gggaggaaga
    39541 ggtaaaagtt tatgcccaaa ctcctcgcat aaaagttcta acttacccat tctatggaat
    39601 cttgcattat ccataataat aaccgatggt gtggttaatg ttggtaagag aaacttctga
    39661 aaccaagctt caaaaaagtc gctcgtcatc gtctcttcgt aagtcattgg aacgattaat
    39721 tcaccatttg ttaga
//
LOCUS       DBERMR010000015        35469 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.015, whole genome shotgun sequence.
ACCESSION   DBERMR010000015 DBERMR010000000
VERSION     DBERMR010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 35469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35469
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.015"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(101..451)
                     /gene="rbfA"
                     /locus_tag="ACL8A1_000832"
     CDS             complement(101..451)
                     /gene="rbfA"
                     /locus_tag="ACL8A1_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HGS1201629.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
     gene            complement(702..3578)
                     /gene="infB"
                     /locus_tag="ACL8A1_000833"
     CDS             complement(702..3578)
                     /gene="infB"
                     /locus_tag="ACL8A1_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HGS1201630.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAAPKASAEKKAE
                     KSEPVKPAVAKEEAKPAEPVTPKTEKVAAKPQSRNFKAEREARAKEQAERRKQNKGNN
                     RDQQQNGNRQKNDGRNGGKQGQSNRDNRRFNDQAKKQQGQQKRRNERRQQEDKRSNQA
                     APRIDFKARAAALKAEQNAEYARSSEERFKQYQAAKEALAQANKRKEPEEIFEEAAKL
                     AEQAQQVQAVVEVVPEKKEPAVDTRRKKQARPDKNRDDYDHEEDGPRKQQKNRSSQNQ
                     VRNQKNSNWNNNKKNKKGNNKNNRNQTPKPVTERKFHELPTEFEYTDGMTVAEIAKRI
                     KREPAEIVKKLFMMGVMATQNQSLDGETIELLMVDYGIEAKQKVEVDNADIERFFVED
                     GYLNEDELVERPPVVTIMGHVDHGKTTLLDTLRNSRVATGEAGGITQHIGAYQIVENG
                     KKITFLDTPGHAAFTSMRARGASVTDITILVVAADDGVMPQTIEAINHSKAANVPIIV
                     AINKIDKPGANPERVIGELAEHGVMSTAWGGDSEFVEISAKFNQNIEELLETVLLVAE
                     IQELKADPTVRAIGTVIEARLDKGKGAVATLLVQQGTLNVQDPIVVGNTFGRVRAMTN
                     DLGRRVKVAGPSTPVSITGLNEAPMAGDHFAVYEDEKSARAAGEERAKRALMKQRQAT
                     QRVSLENLFDTLKAGELKSVNVIIKADVQGSVEALSASLQKIDVEGVKVTIVHSAVGA
                     INESDVTLAEASNAFIVGFNVRPTPQARQQAEADDVEIRLHSIIYKVIEEMEEAMKGM
                     LDPEFEEKVIGEAVIRETFKVSKVGTIGGFMVINGKVARDSKVRVIRDGVVIYDGELA
                     SLKHYKDDVKEVTNGREGGLMIDGYNDIKMDDVIEAYVMEEIKR"
     gene            complement(3595..3894)
                     /locus_tag="ACL8A1_000834"
     CDS             complement(3595..3894)
                     /locus_tag="ACL8A1_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HGS1201631.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLAKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            complement(3887..4180)
                     /locus_tag="ACL8A1_000835"
     CDS             complement(3887..4180)
                     /locus_tag="ACL8A1_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HGS1201632.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            complement(4202..5338)
                     /gene="nusA"
                     /locus_tag="ACL8A1_000836"
     CDS             complement(4202..5338)
                     /gene="nusA"
                     /locus_tag="ACL8A1_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HGS1201633.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            complement(5382..5861)
                     /gene="rimP"
                     /locus_tag="ACL8A1_000837"
     CDS             complement(5382..5861)
                     /gene="rimP"
                     /locus_tag="ACL8A1_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HGS1201634.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVRIPYSLVSKARLAVKL"
     gene            complement(5987..6622)
                     /gene="trmB"
                     /locus_tag="ACL8A1_000838"
     CDS             complement(5987..6622)
                     /gene="trmB"
                     /locus_tag="ACL8A1_000838"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HGS1201635.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDDEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            complement(6619..7413)
                     /gene="ccrZ"
                     /locus_tag="ACL8A1_000839"
     CDS             complement(6619..7413)
                     /gene="ccrZ"
                     /locus_tag="ACL8A1_000839"
                     /EC_number="2.7.1.15"
                     /EC_number="2.7.1.229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0051726 - regulation of cell cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell cycle regulator CcrZ"
                     /protein_id="HGS1201636.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETASDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            complement(7574..8185)
                     /locus_tag="ACL8A1_000840"
     CDS             complement(7574..8185)
                     /locus_tag="ACL8A1_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HGS1201637.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            complement(8336..8569)
                     /gene="blpZ"
                     /locus_tag="ACL8A1_000841"
     CDS             complement(8336..8569)
                     /gene="blpZ"
                     /locus_tag="ACL8A1_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein BlpZ"
                     /protein_id="HGS1201638.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            complement(8611..9300)
                     /locus_tag="ACL8A1_000842"
     CDS             complement(8611..9300)
                     /locus_tag="ACL8A1_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGS1201639.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQMTLIIQNYWQFSSQIGNFV
                     WIQNFLSLLFSGVMIWILVKTGHGYLFHIPRKKWLWYSILTVLVVVLQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYITLFFLTPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFIFATSGIIFASLTRYTKSIYPSYAVHVINNIFATLP
                     FLLTFLHRVLG"
     gene            complement(9352..9735)
                     /locus_tag="ACL8A1_000843"
     CDS             complement(9352..9735)
                     /locus_tag="ACL8A1_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HGS1201640.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            complement(10364..10722)
                     /locus_tag="ACL8A1_000844"
                     /pseudo
     CDS             complement(10364..10722)
                     /locus_tag="ACL8A1_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092061.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
     gene            complement(11203..11322)
                     /locus_tag="ACL8A1_000845"
     CDS             complement(11203..11322)
                     /locus_tag="ACL8A1_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PncF family bacteriocin immunity protein"
                     /protein_id="HGS1201641.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            complement(11426..11575)
                     /gene="blpO"
                     /locus_tag="ACL8A1_000846"
     CDS             complement(11426..11575)
                     /gene="blpO"
                     /locus_tag="ACL8A1_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HGS1201642.1"
                     /translation="MNTKMMSQFSVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            complement(11819..12067)
                     /gene="blpK"
                     /locus_tag="ACL8A1_000847"
     CDS             complement(11819..12067)
                     /gene="blpK"
                     /locus_tag="ACL8A1_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpK"
                     /protein_id="HGS1201643.1"
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGAAVVAAL
                     GCAAGGVKYGKILGPWGAAIGGIGGAVVCGYLAYTATS"
     gene            complement(12136..12405)
                     /gene="blpJ"
                     /locus_tag="ACL8A1_000848"
     CDS             complement(12136..12405)
                     /gene="blpJ"
                     /locus_tag="ACL8A1_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HGS1201644.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLAGAFVGAHIGAIGGGLTCLGGMVGDKLGLSW"
     gene            complement(12872..13069)
                     /gene="blpI"
                     /locus_tag="ACL8A1_000849"
     CDS             complement(12872..13069)
                     /gene="blpI"
                     /locus_tag="ACL8A1_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="HGS1201645.1"
                     /translation="MNTKMMEQFSVMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLICVGSGIMSSL"
     gene            13351..13938
                     /locus_tag="ACL8A1_000850"
     CDS             13351..13938
                     /locus_tag="ACL8A1_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HGS1201646.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            13883..14607
                     /locus_tag="ACL8A1_000851"
                     /pseudo
     CDS             13883..14607
                     /locus_tag="ACL8A1_000851"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
     gene            14794..15507
                     /locus_tag="ACL8A1_000852"
     CDS             14794..15507
                     /locus_tag="ACL8A1_000852"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS1201647.1"
                     /translation="MGDIEFDDLSYKYGFGRDTLTDINLTIKQGDKVSLVGVSGSGKT
                     TLAKMIVNFFEPYKGHISINHQDIKNIDKKVLRRHINYLPQQAYIFNGSILENLTLGG
                     NHMISQEDILKACELAEIRQDIERMPMGYQTQLSDGAGLSGGQKQRIALARALLTKAP
                     VLILDEATSGLDVLTEKKVIDNLISLTDKTILFVAHRLSIAERTNRVIVLDQGKIIEV
                     GSHQELMQAQGFYHHLFNK"
     gene            15518..16879
                     /locus_tag="ACL8A1_000853"
     CDS             15518..16879
                     /locus_tag="ACL8A1_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HGS1201648.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMDESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLANSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LVASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTSPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            16936..17064
                     /gene="blpC"
                     /locus_tag="ACL8A1_000854"
     CDS             16936..17064
                     /gene="blpC"
                     /locus_tag="ACL8A1_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HGS1201649.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDLLYNINRYAHYIT"
     gene            complement(17135..18475)
                     /locus_tag="ACL8A1_000855"
     CDS             complement(17135..18475)
                     /locus_tag="ACL8A1_000855"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGS1201650.1"
                     /translation="MNIAWILLYTLVTNGLEIVIFFKVDGIDLTFERIFKAFLFKILL
                     AFVFVMISYIVGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRKEILDKAFQK
                     SLTQINWIMGGYYLVMESLSFFEYEQSIQSKTVRHLILVFYLLFFMGVIKKLDTYLKE
                     KLYERLEQEQALRYRDMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EVYGSVLKDSSQKLQNNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTIVSILCDNAIEASVEASQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQNQVFRQVLTVIHTE"
     gene            complement(18489..19226)
                     /locus_tag="ACL8A1_000856"
     CDS             complement(18489..19226)
                     /locus_tag="ACL8A1_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGS1201651.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHKKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRAHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINKLH"
     gene            complement(19231..19569)
                     /locus_tag="ACL8A1_000857"
     CDS             complement(19231..19569)
                     /locus_tag="ACL8A1_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HGS1201652.1"
                     /translation="MKYMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLEEGE"
     gene            19845..20174
                     /locus_tag="ACL8A1_000858"
     CDS             19845..20174
                     /locus_tag="ACL8A1_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HGS1201653.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(20342..21391)
                     /locus_tag="ACL8A1_000859"
     CDS             complement(20342..21391)
                     /locus_tag="ACL8A1_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1201654.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKVVQKVSGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSLLAQVFIEQSWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLISFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(21388..22119)
                     /locus_tag="ACL8A1_000860"
     CDS             complement(21388..22119)
                     /locus_tag="ACL8A1_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1201655.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            22187..22597
                     /locus_tag="ACL8A1_000861"
     CDS             22187..22597
                     /locus_tag="ACL8A1_000861"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HGS1201656.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            22607..22894
                     /locus_tag="ACL8A1_000862"
     CDS             22607..22894
                     /locus_tag="ACL8A1_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201657.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIINQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            23056..23295
                     /locus_tag="ACL8A1_000863"
     CDS             23056..23295
                     /locus_tag="ACL8A1_000863"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201658.1"
                     /translation="MHHNLGNSYSIKIKKQTRKLGTDCSKHRFKVVDRTDEVSSKHCF
                     EVVDRTDEVSNHTHDKPTLTWFEEIFEEYHSPFHN"
     gene            complement(23617..24744)
                     /gene="dnaJ"
                     /locus_tag="ACL8A1_000864"
     CDS             complement(23617..24744)
                     /gene="dnaJ"
                     /locus_tag="ACL8A1_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HGS1201659.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFNGAGGFGGFEDIFSSFFGG
                     GGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGTSPV
                     TCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAHSVH
                     VKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLNLNF
                     VQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVNVVT
                     PTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(25146..25502)
                     /locus_tag="ACL8A1_000865"
     CDS             complement(25146..25502)
                     /locus_tag="ACL8A1_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N7)-methyltransferase"
                     /protein_id="HGS1201660.1"
                     /translation="MSVLDEEYLKNTRKVYNDFCNQADNYRTSKDFIDNIPIEYLARY
                     REIILAEHDSCIKNDEAVRNFVTSVLLSAFVSAMVPAMISLEIQTYKFVIPFIIGMIW
                     TVVVFLMINWNYIGKY"
     gene            complement(25504..27327)
                     /gene="dnaK"
                     /locus_tag="ACL8A1_000866"
     CDS             complement(25504..27327)
                     /gene="dnaK"
                     /locus_tag="ACL8A1_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HGS1201661.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(27807..28331)
                     /gene="grpE"
                     /locus_tag="ACL8A1_000867"
     CDS             complement(27807..28331)
                     /gene="grpE"
                     /locus_tag="ACL8A1_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HGS1201662.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(28358..29392)
                     /gene="hrcA"
                     /locus_tag="ACL8A1_000868"
     CDS             complement(28358..29392)
                     /gene="hrcA"
                     /locus_tag="ACL8A1_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HGS1201663.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFHRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVHLLNFANLEAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(29556..30065)
                     /locus_tag="ACL8A1_000869"
     CDS             complement(29556..30065)
                     /locus_tag="ACL8A1_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HGS1201664.1"
                     /translation="MNSRVEFRIFTIVDLDKEEEHLHEMHLKGWRYRTSRFGLFYFDQ
                     CQPDDVIYRIYDSRFLKKYKHELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTTTFLGGLVVCTSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            30673..33006
                     /gene="hsdR"
                     /locus_tag="ACL8A1_000870"
     CDS             30673..33006
                     /gene="hsdR"
                     /locus_tag="ACL8A1_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HGS1201665.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFKPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKIIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDANGQLIED
                     RYYSRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGEPVKVLE
                     TGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            33019..34482
                     /locus_tag="ACL8A1_000871"
     CDS             33019..34482
                     /locus_tag="ACL8A1_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGS1201666.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSNM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLDPKLGESMVDLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGAELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            34482..>35469
                     /locus_tag="ACL8A1_000872"
     CDS             34482..>35469
                     /locus_tag="ACL8A1_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGS1201667.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRFSTLVEIVRG
                     GSPRPIKDYLTSEVDGINWIKIGDTEKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLT
                     NSMSFGRPYILNVDGAIHDGWLAISNYENSLNKDYLFYILSSNVVYSQFLSLISGAVV
                     KNLNSDKVASILIPLPPLSEQQRIIEAIESALEKVDEYAESYNRLEQLDKEFPDKLKK
                     SILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQ"
BASE COUNT        11080 a         7394 c         6347 g        10648 t
ORIGIN      
        1 aggcgtacta ttctagtttc aatatatttt aaaagaatat ttttccattt caaattcaac
       61 ctattttcct ccaccaaaaa agaggggcaa cccctcttct ttagttctta tccagattgc
      121 gtagcatctc gtcaatcttg tttccatact cgatggactc gtctttgacg aaggtcaaat
      181 ctgggatttt gtacaatttc aaattgcgac caagttcacg tttgatggta ccagttgctt
      241 tttcaagccc gatttgggct ttttggttat ccgaagcaag gttactcaaa atggtgtaat
      301 aaaccttggc aacagacaag tcacccaaca tctgaacatc tgtgatggtc acaccttgga
      361 cacgtggatc acgaactttc ttttgcaaaa tctcattgac ttcacgcttg atttccatgc
      421 ccacacgatc tgtacggaaa tgatttgcca tgatattcct ttctctactt tgaaccaaaa
      481 gctaggccag cagacctagc tattttaaac ttcttgattt tcagtttaaa ttgaaacgaa
      541 gttcaatttg aagtcatctg cttctttcgc cgtggtgaaa gtgatggaac tgatttatca
      601 gtcccatcac aggggatttt tgagacccta ggctcaaaaa taagcaatga aaccatcggt
      661 ttgcttgcgt ccctcaccac ctaagaaagg agcaaaaaat cttatctctt gatttcttcc
      721 atgacatacg cctcaatcac atcatccatc ttaatatcat tgtagccgtc gatcatcaat
      781 ccaccttcac ggccgtttgt aacttcctta acgtcatctt tatagtgttt caagcttgcg
      841 agttcaccat catagataac gacaccatca cggataacac ggactttaga gtcacgggca
      901 accttaccgt tgataaccat aaatccaccg atagttccca ctttagacac cttgaaggtt
      961 tcacggataa ccgcttcacc aataactttt tcttcaaatt ctggatcaag catccctttc
     1021 atagcttctt ccatctcttc gataaccttg tagataatgc tgtgaagacg gatttccaca
     1081 tcgtcagctt ctgcttgttg acgagcttgt ggtgtagggc gtacgttgaa accaacgata
     1141 aaggcatttg aagcttcggc aagggtcacg tctgattcgt tgatagcacc gaccgctgag
     1201 tggacgatag tcactttgac accttccacg tcaatctttt gaagtgaggc agaaagggct
     1261 tcaacagaac cttgtacatc agccttgatg ataacattaa cagatttgag ttccccagct
     1321 ttaagggtat caaagaggtt ttcaaggcta acacgttggg tagcttgacg ttgtttcatg
     1381 agggcacgtt tggcacgctc ttcacctgct gcacgcgcag atttttcatc ctcgtaaacg
     1441 gcaaagtggt cacccgccat cggtgcttcg ttcaaacctg tgatagagac tggtgttgat
     1501 ggtccagcaa ctttaacacg acgaccaagg tcgttggtca tagcacggac acgaccgaag
     1561 gtatttccga caacgattgg gtcttgaaca ttcaaggtac cttgttgtac aagaagggtt
     1621 gcgaccgcac cttttccttt atccaagcgc gcttcgataa ccgtaccgat tgcacgcact
     1681 gttgggtctg ccttgagttc ttggatttca gctacaagaa ggactgtttc caacaattct
     1741 tcgatatttt ggttgaattt agctgagatt tcaacaaatt cagaatctcc accccaagcg
     1801 gttgacatca caccatgctc tgccaattca ccgataacgc gttctgggtt agcacctggt
     1861 ttatcaatct tgttaatggc tacgatgatt ggaacgttag ctgcttttga gtggttgatg
     1921 gcttcaatag tctgaggcat aaccccgtca tctgccgcta cgaccaagat cgtaatatcg
     1981 gtaacagaag caccacgcgc acgcattgat gtaaaggccg cgtgtcctgg tgtatcaagg
     2041 aaggtaatct tcttaccatt ttccacgatt tggtaggcac cgatatgctg agtaatacca
     2101 cctgcttcac ctgtcgcaac acgtgagtta cgaagagtat ccaaaagggt tgttttaccg
     2161 tggtcaacgt gtcccatgat agtaacaact ggtggacgct caaccaattc atcttcattg
     2221 agataaccat cttcgacaaa gaaacgttcg atgtcagcat tatccacttc aaccttttgt
     2281 ttggcttcga taccgtaatc caccatgagg agttcaattg tttccccatc caaggattgg
     2341 ttttgtgtgg ccatgacacc catcatgaaa agtttcttaa cgatttcagc tggttcacgt
     2401 ttgatacgtt ttgcgatttc cgcaacggtc ataccatctg tatattcaaa ttctgttggc
     2461 aattcatgga atttacgctc cgtaacaggt tttggagtct gattacggtt gttcttgtta
     2521 ttgccttttt tgttcttttt gttgttattc cagttactat tcttttgatt tctcacttga
     2581 ttttgactac ttcgattctt ttgttgtttt ctaggaccat cttcttcatg atcataatcg
     2641 tcacgatttt tgtctggtcg agcttgtttt ttacgacgtg tatccactgc aggttctttt
     2701 ttctcaggga cgacttcaac cactgcttga acttgctgtg cttgttcagc taacttagcc
     2761 gcttcttcaa agatttcctc tggttccttg cgtttgttag cttgagccaa ggcttcttta
     2821 gcagcctgat actgcttgaa gcgttcctca cttgaacgag cgtactctgc attttgctct
     2881 gcttttaggg ctgctgcacg ggctttaaag tcaatacgtg gagccgcttg atttgaacgt
     2941 ttatcctctt gctgacggcg ctcatttcta cgtttttgct gaccttgctg cttcttagct
     3001 tggtcattaa agcgacgatt gtcgcggttg ctttgacctt gttttccacc attacggccg
     3061 tcgtttttct gacggtttcc gttttgttgt tggtcacggt tattgccctt attttgcttg
     3121 cgtcgctctg cctgctcttt agcacgtgct tcacgctcag ccttgaaatt acgactttgc
     3181 ggtttagccg ctactttttc tgttttcgga gtgactggct cagctggttt tgcctcttcc
     3241 ttggctacag ctggttttac tggttcagac ttttcggctt tcttttctgc acttgctttt
     3301 ggggctgcag gttttgcctc ttccttagct acagctggtt tagctggctc agatttttcg
     3361 gctttctttt ctgcacttac ttttggtgct gcaggttttg cttctacttt cggagcagct
     3421 gcaggcttaa agctggcagc aatttttgca gcgacagctt cttccacact tgatgagtgg
     3481 cttttcacat ccaagcccaa ctcttttgca cgcgctacaa cttctttact ttcttttcca
     3541 agttcttttg cgatttcgta caatcttttc ttagacaaat catgtcctcc tcttctattc
     3601 cataagagac ctcattttct ttgtaaatcc agcatctgtt acagccaaaa cctttctcga
     3661 tttcccgact gctatgctta attccagtgt tgaaaacacg gttacaattt ctacttgata
     3721 ataatgactt ttatcttgaa tcttcttggc cagattgggt ccagcatcat gagctagaaa
     3781 gaccaacttg gccttgccgt cttgaatggc cttgaccacc aattcttcac ccgatatgat
     3841 gcgccctgct cgctgagcaa gccccaagag attacttatc ttttgcttat tcaagtccca
     3901 actctcttct tttcactttg tgatccacat aagcgatcaa ctcgtcataa aagctttctt
     3961 ccacttccat gctaaagctg cggttaaaga ccttcttctt tttcgcctct agggcttctg
     4021 cattgtctag tttgatataa gcgccgcggc cattggcctt gcccgtagga tcaataaaga
     4081 cttgtccttc cttgttcttg acaatgcgga gcaaatcacg cttatcaatc acttcgttag
     4141 acacaacaga cttgcgcaaa gggatttttc ttgttttcat ctttccctcc tctagcagct
     4201 tttattcttc tacagtatcg ttttctactt ccaactctac tgaagcagcg tcttccatgg
     4261 cttcaaattc gctagcagac ttgatatcga tacggtaacc agtcaagtga gccgccaagc
     4321 gcacgttttg tccacgacga ccaatggcaa gagaaagctt gttatctgga acaaccacca
     4381 aggcacgttt gctgtcgttt tcatcaaaga taacttggtc aacctcagca ggagcgatgg
     4441 cattgtagat aaattcagct ggatctgcta cccactcgat aacatcgata ttttcttcga
     4501 ttggtaccat gcggtcattt ttagcatcgt aacgagctgg gtggaatttg ctagtaatct
     4561 tcttgatatt agcaccacca cgtccaacga ttgtaccgat agcatccacg tttggattgt
     4621 ggctacgaac agcaaccttc gtacggtcac ctgcttcacg agccacgctc atgatttcaa
     4681 cagttccatc ataaacttct ggaatttctt gctccatcaa acgtttgatc atttctggat
     4741 ggctacggct aacaaagacg ttcacaccac gagggttgtc ttcaaccttg taaacataaa
     4801 cttcgatacg atcatgagaa gcaaaaactt ctccaggaat ttgatcttgt tttgacaatt
     4861 gggcttcgat gctaccaagg ttgacataga taaagcggtt gtcaaagcgt tctactgtac
     4921 cagacatgat ttcttgctca tgttctttgt aagtattgta agtgatggca cgtgtttgct
     4981 tgcgcatttt ttccatgatg gtttgtttgg cagattgggc tgctacacga ccaaactcag
     5041 ctggtgcttc ttcaaacttg attttgtctc caagttcata agctgaatta atggcaagag
     5101 catctttcaa gctgatttcc aaacggctat caaatacttc atcaacaact tcacggacag
     5161 tataaactgt aaagtcacct gttttttcgt tgaagtcaat agctacgctg tctgactgac
     5221 catagcgtct gcgataagcg gaacgaagcg actctactac tgcgtcgatg atatcttctt
     5281 ttttgattcc cttgtcttct tccaaaatgc ggaaggcctc tagcatttct ttactcatgt
     5341 tttcttcact tttgaatcct caaaagctat cctttctttt tctataattt aactgctaaa
     5401 cgtgcttttg atactaaact gtatggaatt cggacggttt tcttacgcgt cttgtccata
     5461 tattccatag tcaactcgtc ctcttcgaag gccaacaaag ttccttcaaa gaccttttgc
     5521 ttatcgatgg cttggtagag tccgacatgg atgtattttc caaccgctcc agcgacggca
     5581 tccttggttt tcaaaggacg ttccaagcct ggactggtaa tttctaggaa atattgttct
     5641 gggaagggat ctggcttgat ggtgtctagg acaggactga taatttctgt caagtctgcc
     5701 gtgtcgttca aggtaattcc ttcgggttta tctacaaaaa tactgagaat catgtcactg
     5761 ccaatctttc catactcgat atccacgagt tcaaaaggag cttctatgac aggttctaca
     5821 acttctctga ctaattctac gattgttgcg attgcgtcca cctcctcata agcaagaggc
     5881 gaagatattt ccccgcctca cttttcatat tcttattatc agtatagcac gtttgccaac
     5941 ttatgtaaag caaaagcact tgtacagcct aattttaggc tatctcttaa aattctgcct
     6001 caactcggta gataacttgc cccttgtttg agaatttttg ctcgtattct gtcatgacat
     6061 tgccttcaaa atcactggca tgcaaatcta gccagacacc attgagtttc atgccatatt
     6121 gagaaaagct cactaaactg tactcaaaca agccacggtt atccgtcttg aaatgaattt
     6181 ctccattttc aggcaagata cgtttgaagg tgtccaaaaa ggtcttgtag gtcaaacgac
     6241 gcttttcatg gcgttttttc ggccatggat ctgaaaagtt cagatacaag cgatcaatct
     6301 catcgtcttc aaagtagtca gtcaagtcag aaccatctac ccacaagagt ttaatgttag
     6361 gcactccaac ttcaagcacc ttgtccaaag cgtagctcaa aacagacttt tgaatatcaa
     6421 tcccgatata gttgatgtca gggttttgct tggccatacc tgaaacaaag gcaccctttc
     6481 cacttccaac ttccacatga atgggattat cattgccaaa caagtcccgc cattttgcct
     6541 tggcttccaa gggattgagg accacatact ggggatttgc ctctagtaat tctgttgccc
     6601 ctttacgatt tctaactctc atcttctctt tccatacttg tctcggaaat gacgcaaacc
     6661 atgaatctcc cgattgacat tttctaaatc ttggttcata taatacttgg aaatctgact
     6721 caaataagac aattgaccat accaatacaa tttacttaat accgtttgat tgtacttgta
     6781 accgtagtag gtcaaccatt ccttccactg atgttctgaa atataatggc agagcatatg
     6841 ggccacatca aacatgcgat cggtcaagcg aaccgaatcc caatctacta aataaatcaa
     6901 gccactatct gtctcaatcc aattactatg tcgtacatct ccatggacaa tggtcgcatg
     6961 gtcctctcta aatcctggaa tagtctgacg taaatcagcc atcacttcac tgataaaatg
     7021 atttttacgc aaagcatctg aagccgtttc ttgccaagac tgtagtaaat ctacaggtgt
     7081 ttccatggca tagcccaaac gactcaattg tgtcatcaac ggacgtgagc gatgaagacg
     7141 ggttaaaata ttgacgattt gcttacgatt catatcatag ggggtcaata tcttgcctgt
     7201 caaccattct tgagcacaca tatcacgccc atctgccaaa cgacgactcc ataataattg
     7261 tggagcaatt tgttctctag ctagaccagg taggattgga gaggtgttca tttttacaaa
     7321 gatgcgcttc ccgtcaggat agctacccat ataagccttg ccacttttcc caggtatggg
     7381 agtcagtgtt agctcattat cacccaaatc catttctttc ctccgtataa agtttcctta
     7441 ctattctact agtttttggc ctattcgtca accactctaa cccgattctc aaagaaaagc
     7501 ctctggattg gcttgggtat cattatagct tgaaaaaggt aagcaccatc ttctgcttac
     7561 cttttcataa tttttataaa taatatagga gtggccaact gttgtaaaac atgtggacta
     7621 gagtagaaac ccataggttt tgactctttt tataagcaaa gcccagcaac aaacccccaa
     7681 gtagataaaa aataaacgaa gggacattag aaggtccatg catgaaagaa aagagagagg
     7741 aagttagcac aagccctaac caagaattgt caaaaaccgc accttgaaga agtcctctga
     7801 aaatgagttc ctcctggatg gggccaaaaa ctgaggtagt caaaatcaaa gacagggaaa
     7861 ttcctctgct gacttccgcc aagtgttgga cccctgagcc agaagaaaca gcgaagaaat
     7921 gaatataaac cagcgtctca agtacactta gaagaaagat tgcaatgaaa agcaaaagtt
     7981 gtttcttgct taacatccca aaagaaatca tttcaaactt tctagcataa gcaacactca
     8041 gcgaggttac aagaagactt acaagaatca atgcagattc attttgaaaa aaacccccat
     8101 ggttaatcgt ataaggaact gtaatgacaa ttattagtac taaaaatcca aaacacaagg
     8161 catgaaattt atcaaaaaac tccataaaac tatcctcctc accaaacaga cttcctacgc
     8221 gtcattcttt agttctagcc tttccaaaac aaaccatttt atactcttcg aaaatcaaat
     8281 tcaaaccacg tcaacgtcgc cttgccgtac tcaagtacag cctgcggcta gtttcctagt
     8341 ttgctctttg attttcattg agtattagta accaaaatcc gaccacatag ccagccccta
     8401 tgaatatagc cattaaagct agcatggaat ttaggaaatt aaaaaccacc gcagatacaa
     8461 aggttagcac aaaaacatta aaagcaatgg tgtcagaagc caagactaga atatagggtg
     8521 tcaaccgatc taaagttttg gaatctagga aaaataagtg tttatacatg ataatctcct
     8581 ctatggctga aaagcaagcc ttttgttttt ttaccccaag accctatgta aaaaagtcag
     8641 caaaaatggt aatgttgcga aaatattatt gatcacatgc accgcatagg aaggataaat
     8701 gctcttggta tagcgggtca aactagcaaa gatgatgcca gatgttgcaa agatgaagat
     8761 atctaacaaa ctaggcaggc ttgaaaaatg aggaagagca aataaaattg acggaagaag
     8821 caaatcaagg ccaaatctcg aatgcttaaa aaaggcgtgt tgcagtaatc ctctatagat
     8881 tagctcttcc ataagtggag tcagaaagaa caaagttata tagataccta gctcagcaaa
     8941 gttggtccca ctataaccga tcaatacagc ccaaccttca gcagttgact gaacatgttt
     9001 agctgtctga acgttaaaag agatctggag cactaccact aatactgtca aaatcgaata
     9061 ccaaagccat ttttttcttg gaatgtgaaa gagataacca tgacctgtct taaccagaat
     9121 ccaaatcatg actccgctaa atagcaaact caagaaattt tgaatccaga cgaaattgcc
     9181 aatctgggaa gaaaattgcc aataattttg aataataagc gtcatctgag aaagaccaaa
     9241 tacgaaaaat aagtaagaga aaactgcact tattttgaat agaagttgat actttttcat
     9301 agaaatcctc cctactatga cctcaccttg tcaggctcta ctgctgtaag attaagaaga
     9361 cagtttgttt tttttaaggc taacctgact actagataat agatacatta aggcattaaa
     9421 gacaatgaaa atatgtccat agaataaaat caacctcgca tccaaaccaa gataaagttt
     9481 gattatcaaa aagatgagca aaagaatttg aaaccataag gtttttccaa aaataaattt
     9541 aaagcgattt cgaatatcta cttccttgat ttttaccgcc acccctttat tagcaagaag
     9601 gaaaactcct gcttcaaaca aaccactgta aagaacaagc cacccaatag atacgataga
     9661 gatttgtaaa aatgtcccta aaagaatatc caacacacta ctcaagaaaa taacaaaaaa
     9721 taatctgtat ttcatattaa atacctccat tcatttattt cactaacaat ttaatagagc
     9781 cttctactca aatatcctgt cagaaaagga tagaaagcta ctttttataa tacttcaagc
     9841 cccacatgag cagaagcgtg ataaacaagc agagaataca cctatataag cgattagttg
     9901 ttgatagaat tctgtttctg aaatacctct atacaaacaa atgacaaaca taaaatctgc
     9961 caagccgata aacataagtt gattggttct aggactaacc aaatcatcat ttacttatat
    10021 ttaagagtat ctcttttatg ttaatgtatg ttagcactga aaagcaagac aggccaataa
    10081 tatttaaaat gaacagtaac ggggttaagt ctctaaaaaa attatctact gacactacaa
    10141 gaaatactat acatattata gtcgaaacta tctttttctt atccataatt atttactcct
    10201 ttcctaacaa atccaactta tcaatcaaga gcgattttta acataatgta gcagcacccg
    10261 ttgcaacttt gacaagttta gtatatcatt gttttttaaa atttttcatc caaatcttga
    10321 attgtcatcg aaacctccta aattgcaaaa acttcattag cccctacttt ttataatact
    10381 tcaaacccca aatgagtaga agcatgatga tcaagcaaag aatagcacca atataagcga
    10441 ttagttgttg ataagattct cctgctgtga tacctctata caaacaaata atagacataa
    10501 agcctgtcaa gccgataaac atgagttgat tggttctagg actaaccaaa tcatcatctt
    10561 caaactctct taatctcatt tccctagtaa gataaacagt aaccaaaata gaagtcaagt
    10621 taataactac taaaagaaat tggaaaactg acgaaataga aatagataag tagaaacaag
    10681 caaagacaac tgacccagga acaatctcac aaagaatatg attcttttta atcaagaaaa
    10741 tctttcattt cttttctttc tttttaatta aagaaaaata gatcataacc gcaatcatat
    10801 aggctatggt ataaaataga ttataccaaa tactctccct aagcggataa agaaagatgg
    10861 acatgatcag atacagaacg aaaataatca gtattttttt cttcataagt tttcctccta
    10921 aatgtgcgat ttatcttagt tgagcaagaa catttacact gctagtatag cacttatttt
    10981 gaccttggat cactcaaatc ataaatggtc atcaaaacct cttgaattgt aaaaattaaa
    11041 aaagcaagca tgaaaaacat actttcctct ttatattgta ttgataccaa cttgtttgta
    11101 gacttttcat cctgctatca catatcattt tgacaggcga aacaatatta aagaaactcc
    11161 cctgtaaatt aagctagcaa atacagggga gaaatttatt ttttagagag tactatccgt
    11221 atcctttttg gaagattttg aaaatatttt tctaattaag tcatccatat aaggaccaaa
    11281 tataccaact actaaaccaa taataaaact tttaaaatcc ataattacca ccaacatgtt
    11341 gctgcatagg ctacacctcc aagtatagct ccacctgcag caccagttac acctattcct
    11401 atagcaaatg gtcccaatag aaatgtcaaa ccgttgttgc acacccatca attgcgccat
    11461 atgcaacccc tgctgcacaa ctaatttttc ttccccaatc aatatctcca ccttcaacgc
    11521 aagcaagcat ttcattatcc ataacagaaa attgtgacat catttttgta ttcatatagt
    11581 atgtcacttt tcagttacgg aacaagttta atataaaaat tatcaaaaaa acataggcaa
    11641 taaagagaaa aattaattta tcatagatta gaaataatat gacaaaacaa ttcaatgatg
    11701 ttaattcaat agtcttttgt tttttatcag agatacttat ggatagataa ataagatagg
    11761 tttgaaaagc gaagagaata ataaagaata tagccttcat aaaatttagc tttcattttt
    11821 atgatgtagc ggtataggct aaatatccac aaaccactgc tcctccaatt cctcctattg
    11881 cagcgcccca tggtcctaga attttcccat atttcactcc acccgctgca caacctaaag
    11941 cagcaactac agctgctcct ccaacacctg ctttggcaaa atctccccaa ttgcatccgc
    12001 caccttcaac gcaagcaagc atttcagtat ccataacaga aaattgtgac atcatttttg
    12061 tatccatgac aaatactcct tttttaaaaa actaaaataa atcagaatag aatcctcata
    12121 attttactat aagtcttacc aacttagtcc caatttatca ccaaccatac ctcctaagca
    12181 tgttaatcca cccccaattg caccaatgtg tgctccaaca aatgcaccag caagtccagc
    12241 tactcctaaa gtggccaaac ctgctccagt tccaccagtt ataattcccg tagtgactcc
    12301 tgtaatcagt gcattttgac aatcagtgga gctatacccc ccttcaactt tcgcaagcat
    12361 ttcagtatcc ataacctcta actgtgacaa catttttgta ttcatgatga atacctcctt
    12421 tttattttca atttgttacc aaagtcttaa attcaataaa caaatagatt ttttttagta
    12481 tctttttgat tttcttaaaa aagtatatac gtctactatc ttcttaaagg tagcagtacc
    12541 tattttttag tctaagattt caataatctt gagtatctaa aatatcttaa tttcgttatt
    12601 ctccttgcaa taaaaagttt tactatacta tttattaact tgcagaaagc aaaaaatatt
    12661 agtaaataat agtttatagt taagtttttt attcctacca atccatcaac taagtaaagc
    12721 atcaacgatt acataaacga ttgataatat aattaaaatt ttgctaacta tcttattctc
    12781 atgattctta gataactttg atattttgta agtaagtaaa taagacagta aattaatagc
    12841 gataataata ctatatttaa gaatcataat cttacaaaga ggacataatt cctgaaccta
    12901 cacaaataag tgttgctgct cccccagtta tcggaccagt cgcagcagct aatagtactg
    12961 ctccaataca accaccgatt gcagatccta aattgcctct tcctccacta actatttcga
    13021 gttcttcatt atccataaca gaaaattgtt ccatcatttt tgtattcatg acaaatactc
    13081 cttttttctt tttttatttt tgtcttgttg taactttgat aagtttagta tatcatcgtt
    13141 ttttaaaatt tttcatccag atcttgaatt gtcatcgaaa cgtcttgaat taggtttttt
    13201 atttcaagcc acctctaaat gtttaaaaaa aataatttct aatcactttt ttaccattca
    13261 ggaagtttta atgactattc aagatttcat aaaatatgaa cttagtttta tgacataata
    13321 gacctatcca ctatatgaaa ggaattgcca atgacttctt ataaacgtac atttgttcct
    13381 caaatagatg cgagagactg tggtgtcgct gccttagcct cgattgctaa attctatggt
    13441 tcagattttt ctctagctca cttgagagaa cttgcaaaga ccaataaaga agggacgact
    13501 gctcttggca ttgtaaaagc cgctgatgaa atgggctttg aaacaagacc tgttcaagca
    13561 gataaaacgc tctttgacat gagtgatgtc ccctatccat ttatcgttca cgttaacaaa
    13621 gaaggaaaac tccaacatta ctatgttgtc tatcaaacaa agaaagacta tctgattatt
    13681 ggtgatcctg acccttctgt aaaaatcact aaaatgtcaa aagaacgctt tttctatgaa
    13741 tggactggag tagctatttt tctagctacc aaacccagct atcaacccca taaagataaa
    13801 aagaatggtc tactaagcaa gcttccttcc tctgattttc aaacaaaaat ctctcattgc
    13861 ttacattgtt ctctcaagct tattggtcac tattatcaat ataggtggtt cttactatct
    13921 ccaaggaatc ttggatgaat acattccaaa tcagatgaaa tcaactttag gaatcatctc
    13981 agttggtctg gttatcacct atatcctcca acaagtcatg agcttctcca gagattatct
    14041 cctaaccgtt ctgagtcaga gattaagtat tgatgtgatt ttatcctata ttcgccatat
    14101 ttttgaactt cccatgtctt tctttgcgac acgtcgtaca ggagaaatca tttcacgatt
    14161 cacagatgct aactctatta tagatgcctt ggcttctacc attctttctc tttttctgga
    14221 tgtttctatt ctgattcttg taggaggcgt cttactggca caaaacccta atctcttcct
    14281 tctttctctt atttccattc ctatatacat gttcatcatc ttttctttta tgaaaccttt
    14341 cgaaaaaatg aaccatgatg tcatgcaaag taattctatg gttagctctg ccattatcga
    14401 agatatcaac gggattgaaa ctataaagtc gctcacgagt gaagaaaatc gctatcaaaa
    14461 tatagacagc gaatttgtag attattggaa aaatccttta agctcagtaa atattctatt
    14521 ttacaaacga gtttaaagca gggaacaaaa ttagttctga atatccttat cctatggttt
    14581 ggcgctcaat tagtcatgtc aagtaaaatt tctatcggtc agctgattac ctttaacaca
    14641 cttttttctt actttacaac tcctatggaa aatattatca acctccaaac caaactccaa
    14701 tctgcgaagg tcgctaataa ccgtttgaac gaagtctatc tagtcgaatc tgaatttcaa
    14761 gttcaagaaa accctgttca ttcacatttt ttgatgggcg atattgaatt tgatgacctt
    14821 tcttataagt atggttttgg acgagatacc ttaacagata ttaatctcac gattaaacaa
    14881 ggagataagg ttagcctagt tggagttagt ggttctggta aaacaacttt agccaaaatg
    14941 attgtcaatt tctttgaacc ctacaaaggg catatttcca tcaatcatca ggatattaaa
    15001 aacattgata aaaaagtctt gcgccgtcat attaattacc taccccaaca agcctatatc
    15061 tttaatggct ctattttgga aaacttaacc ttgggcggta atcatatgat tagccaagaa
    15121 gatattctaa aagcttgtga attagctgaa attcgtcaag acattgaaag aatgcctatg
    15181 ggctatcaaa ctcagctctc tgatggagct ggtctatcag gaggacaaaa gcaacgaatc
    15241 gctctcgctc gtgctctttt aactaaagct cctgttttaa tactagatga agctactagc
    15301 ggtcttgatg tcttgactga gaaaaaggtt atagataatc ttatatccct aactgataaa
    15361 accattctct ttgtagccca tcgtctcagt atagccgaac gaactaaccg tgtcattgtt
    15421 cttgaccagg ggaaaatcat tgaagttggt agtcaccaag agttaatgca agcgcaagga
    15481 ttctaccatc atctattcaa taaataagga gaatgtcatg aatcctaatc tttttagaag
    15541 cgtcgagttt tatcagagac gttaccataa ctatgcgaca gtattaatca tacctctgtc
    15601 attactgttt actttcatct tgattttctc ccttgttgcc acaaaagaaa ttactgttac
    15661 ttcccaagga gaaatcgccc ctacaagtgt cattgcatct attcagtcaa ccagtgataa
    15721 tcctatcctt gctaatcatt tagtggcaaa tcaagtagtt gaaaaaggtg acttactcat
    15781 caaatactct gaaacaatgg atgagagtca gaaaactgcc ttagcaactc aattacaaag
    15841 acttgagaag caaaaagaag gacttggaat tttgaaacaa agtttagaaa aagcgactga
    15901 tcttttttct ggcgaggatg agtttggcta ccataatacc tttatgaatt ttactaaaca
    15961 atcccatgat attgaactgg gtatcacaaa gactaacacc gaagtttcaa atcaagctaa
    16021 tcttgccaat agcagttcat cagccatcga acaagaaatt acaaaagttc aacaacaaat
    16081 tggagaatat caagagttga gagatgctat cataaataac agagcacgct taccaactgg
    16141 caatccgcac cagtcaattt tgaatcgtta tcttgtagcc tcacaaggac aaacacaagg
    16201 aactgcagag gagccatttt tatctcaaat taatcaaagt attgcaggtc ttgaatcatc
    16261 tatcgcaagc ctcaaaattc agcaagctgg tatcggaagt gtagcaactt atgataacag
    16321 tttagcaacc aaaattgaag tactccgcac tcagttttta cagacagcct cacagcaaca
    16381 actaactgtg gagaatcaat taacagaatt aaaagtacaa ctagatcaag ccacacagcg
    16441 tttggaaaac aataccttaa cctccccaag taaaggtatc gttcatctga acagcgaatt
    16501 tgaaggtaaa aatagaattc caactggtac agaaattgct caaatattcc ctgtcatcac
    16561 agatacaaga gaagtactaa tcacttacta cgtatcttct gactatctac ctctactaga
    16621 taaaggacaa actgtaagat taaaactgga gaagattgga aatcacggca ccaccatcat
    16681 cggccaactt cagacaattg atcaaactcc taccagaaca gagcaaggaa atctctttaa
    16741 attaaccgct cttgcaaaac tatctaacga ggatagtaaa ctcatccaat atggcttaca
    16801 aggtcgcgtc actagtgtaa ctacaaagaa aacatatttt gattatttca aagataaaat
    16861 tttaacccat tctgattaat tttcagataa cactctataa ctatttatta tcttatcaaa
    16921 aaggagaatc ataacatgga taagaaacaa aacctaactt catttcaaga actaacaact
    16981 accgaactca accaaattac aggtggagga ttgtgggaag atttattata taacattaat
    17041 agatatgctc attacatcac ataagaactt catcatccaa tacaactata aaaaaataag
    17101 accgagaaac aagtactctc ggtcttattt ttcatcattc tgtatgtatc acagtaagta
    17161 cctgacgaaa gacttgattt tgacaggtgg tatttagact ggtattagga tggctttcca
    17221 caatcttcat gacggtatag agaccaactc ctctctcctc ccctttagaa ctggctccaa
    17281 aggagaagat ttcagaaata tcgatgccct cttctttgat ggagttttcg atgataaagg
    17341 tctcctgtgc tccatttttt aaaaaggcga ttgaaacatg aggttgacta gcttccacac
    17401 tggcttcaat agcattgtca caaaggatag acacaatggt tagaaaatca agtagactca
    17461 tcccctcgac ctgaatctcc tcaggaactt cgacattaaa gacaatgttc ttatctctgg
    17521 cttttaaaaa tttccctgct agaagacttt tgagggcttt atcacgaata tttaccaatc
    17581 tgcccaggtc atatttattg ttctgcaatt tctgactgga atcctttaag acggagccat
    17641 agacctcttt tatctgctcc atatcctcct cttcaatgcc cagacgtaag ctagtcaaga
    17701 ggttggtata atcatgacga aagctccgta cttccttgta aagctcctct atatgccgac
    17761 tatagcgttc catatctcta tagcgcaggg cctgctcttg ttccaatctc tcatagagtt
    17821 tttccttcaa ataggtatcc aatttcttga taacccccat aaaaaagagt aggtaaaaga
    17881 ctaggatgag atggcgaaca gtctttgatt gaatactttg ttcatattca aaaaaagaca
    17941 gactttccat gactagatag tagccaccca ttatccagtt aatctgagtc agggactttt
    18001 gaaaggcttt atcgagaatc tcctttctca agctagtaaa atcgtagtcc aaccatttca
    18061 aaaaagctag agaaatgaag aaattgaaaa ttattataca taacccagta aatgagtagc
    18121 catcatatac ttgcccttgt cccaaaaatg gaagcacaaa ataggagact cctctataaa
    18181 agagattcac caatatcatt ggaaagagac cataaaagaa aaggagtttt ttaggaagcc
    18241 ctctcaataa taagaaagat aagcctatgc cgtacaaggg ttccataaaa taagataggt
    18301 aaacatttcc tactatatag ctaatcatca caaaaacaaa ggccaacagt atcttaaaaa
    18361 gaaaggcctt aaaaatcctc tcgaaagtaa gatcaattcc atccacctta aagaagatga
    18421 caatttctag tccattagta acaagtgtat acaacaatat ccaagcaatg ttcataaatt
    18481 ctcctagctc agtgtaattt attgatggcc tcagacactt ccctgacctt ataacgggcg
    18541 attagacaac ttccaccatt gggaaagaaa agcagttttt ctttcttatc caaatgcacc
    18601 acatttgcag gattgatgag aaaagagcgg tggcactgca agagacgggg ctcctgcttg
    18661 aaaacctcct ctaaactcgc tgtaaattcc agcctgtctg tcttggtata gagaataaca
    18721 cgatgggctc tgggcgacgt ttcgagatag taaacctctt taaaaggata ctgaaattgg
    18781 gcaaattttg atttaaagta aaagcaatct tccgccagac ttttactatc ttgactattg
    18841 gcatagagga gggctgtctc gatccgagat tcaaactcct ctgctgacaa ggccttatca
    18901 atgtagtcca aagcagacac ttggtagcga aaggacaggg gcataaactc cgagtgagtc
    18961 gtcacaaaga caatcagggc ataaggatcc cgatcccgaa tctttctagc cacttccagt
    19021 cccttcatct cttcatttcg aatctcaata tccaaaaaga atagctgatg ggcccccttc
    19081 ttatgcactt cagccagcag ttggtccggc ttgccaaata cctcaaaaga gctaggaatg
    19141 atatgatgtg ctttcaaaag tttctcaatc gtcgtttcaa ttctagtctg ttgggaaaaa
    19201 tcatcctcta aaacaaatat tctcatcttc ttactctccc tcttccaacc atttttgacg
    19261 aatttctcta tagcgacgtc tggcatactt gacagcgtat tgaccttctt ctccgagaaa
    19321 aaggattctt tcttgaaaat caatcgattt taatttatca agattaacca aacaatttcg
    19381 atgacatctc atcaaggttt ccccacattg gttctcaaga ttacttaaat tttgaagcat
    19441 attaaaacta gcttcttctg taacaatctg tacggtatgg gctttagttg gatgtgtttg
    19501 gatatagtag atatcgtcga tgtttacttt atagactgtt ttctgcgtct gaataatcat
    19561 atatttcatt ggattctcct cctaagaaaa gaataacaga aaaggtctag cccaagcaca
    19621 cccaatccta aacagtcttt gaaaactcct aaaaggatta ttttctacaa catcatgatg
    19681 agcttacaac tattttatca taaaattcac tcagcgcaat tcaagacatt tcaatgacaa
    19741 ttaaagattt gaataaaaaa aaagaattaa aagtgataaa ctaacagtat caaaacacag
    19801 ttgccagaag taagactggc acccagatta aaggaggcat tcccatgaca gatacagacc
    19861 ccatcaaaag agctcatact ttgattactg acttaaacaa agcctaccaa gcatgcaaac
    19921 aggcaagcgc tgacgacgtc cgctttcagg agcaattaaa ctctattctt ggttttctag
    19981 ccaaggctga aacagtggat aatcgattct tgattgaact agaaaaattt taccagactt
    20041 ccagtcttct catgggactc agcgctcttg atccagatgc tccaactcgc gccgcttggc
    20101 gggcctatga ccgcttccac tttgaccaag tcaagaccaa gttaatactc aatgagaatc
    20161 aaagagcaaa ctaggaagct agccgcaagc tgtacttgag tacggtaagg cgacgctgac
    20221 gtggtttgaa gagattttcg aagagtataa gtctctacgg accaaccatt atcctgtagt
    20281 aacaaaaaag aagctgagac aaactgaact caacttcttt tttagtgtcg tatcagcaat
    20341 gttagtcctg catctgacgt tttacctgat aaggcaaata caagactagt aaaaccaaac
    20401 cagctcctag taaggctaga agggctagtt tttcttggaa ggtaatcaac ccaacaacta
    20461 attccacaag taaaacaaaa ctgatcaatc ctcgaactac ctcctgtaag cctttttcct
    20521 tttgcccctt gtccagcgga aagagttggg tcaaatactg gtagtcaaag gcatgataga
    20581 gggccagcaa ctggaaaagc aagaggtagt taaagagaac taccactgct gtcgcaatcc
    20641 aagattgctc gataaagacc tgcgccagca aagaaagcaa gagcagacgg agactaaggg
    20701 caaaaaggtc tccatttcgc aaataagaac gcagatagag attttgccaa atcttcccag
    20761 acaccttctg aacaaccttt agaataaagt ccagataggc acgacgcttg acgctgtttg
    20821 aaattccctt gacctgcgta aagagggcaa agaaacgaag caagacttgc ttacgcttgc
    20881 tttcttgaga aataacatag tcccagtcca gtccagtttc agtgaaaaat ttgctggcct
    20941 tttgacgaaa gtggaaatat tttcctaccc ccaataaaag cacatagagc agaaaaactg
    21001 gcaagccata acccattgct aaaaataaag gtgcaaataa cagcaagaaa agggtctgta
    21061 caaagagcca aaagactagg gaaatgccag tttgacgctt gagatggagc ttgatttcct
    21121 cttctccaac taagagaaag agcttgtctg gagcctccat ataggtggca attcctcccc
    21181 aaagtaaaag taaaacagac gtaattccta caaacaaaag gataggccaa tgattttcag
    21241 gaaaatgttg taagagttga ctgtactggt aggctagaaa acccaacagg acaagcagga
    21301 acaagacaaa gtggtcattg agcacatagc gcagataacc aagacactcc ttacgaaagg
    21361 cctgctttct ctttaaaaac aagtctttca tagatcctcc tctttggtca aagccaagta
    21421 aatatcattc aaactagcct caggcatatc aaaggcttca cgtagttgca ggagattgcc
    21481 tttggcacgc acctctccct tgtgaagaat gacaaaggca tcacacatct tctccgccga
    21541 atccagcacg tgggtactca tgagaataga cttgcccttt tgcttctcca cttccaaaag
    21601 ctgaatcaaa tcagaaatag ccagcggatc aagaccaagg aaaggctcat ccacgatgaa
    21661 aagacttgga tccaccacaa aagcacagat aatcatgacc ttctgcttca tcccttttga
    21721 aaaatgaaca gggaaccagt ctaatttttg ttccaaacgg aacattttta acaagggctc
    21781 tactcgttcg aaagccactt tttgctcaat accgtaagcc atagcaaccg tttcgatatg
    21841 ctctctgagg gtcaattcct catacagact aggcgtctca ggaatgtagc caatctgctt
    21901 gcggtagcta gtcgcatctc cttgcagagt caggccattg atattgatgg agccactata
    21961 aggtgccaac agaccgataa tctcattgat cgtcgttgat tttccagcac cattgagacc
    22021 aatcaaaccg accaactgcc cactttcaac agtaaaggac acatctttca aaacaggaac
    22081 atgaacatag ccacctgtca ggtttttaat ttctaacata ttttctccaa atctggtata
    22141 atgtagctat attatatcaa aattcagtac agtagaggta gattttatgt cagattgcat
    22201 tttttgtaaa atcatcgcag gggaaattcc tgcttcaaaa gtatatgaag atgagcaggt
    22261 ccttgccttt cttgatatct ctcaagtaac actaggacac accttggtcg tgccaaaaga
    22321 acactatcgc aatcttttgg agatggatgc tacgagcgcc agccaactct ttgcccaagt
    22381 accaaaagta gctcaaaaag tcatgaaagt cactaaggct gctggtatga atatcatttc
    22441 caactgtgaa gaagtcgctg gtcaaacagt ttttcatact cacgttcacc ttgtgcctcg
    22501 ctacagtgct gacgatgacc tcaagattga ttttatcgcc cacgaaccag actttgacaa
    22561 acttgctcaa gtcgctgaaa ccatcaaaaa cgcttaagga gttgctatga aattatccaa
    22621 cctactgcta tttgcaggag ctgcagccgg aagttatctg gttacaaaaa atcgccaaac
    22681 catcacagat gaagtcttga ataccactga ccgcgttcaa gctatcaagg acgatgtgga
    22741 tattatccaa aacagcctgc aaatcattaa ccagcaaaaa gaacttatca aggaatacca
    22801 agaagacttg acttacaagt ttaaggtctt ggaaaaggat atccagacta gactagctgt
    22861 gataaaagaa atgcagggaa ctgaagataa gtaaaaagag cccgacggct cttttttaaa
    22921 acgtaaacaa gttatagtga attgaattta gaatagcaca tcatagtttc taaagcattg
    22981 ttagagatta ctttaaaatc ctcttatcaa tttgttcata ttctatttca atctactata
    23041 tatatcccat caactatgca tcataattta ggtaactcat actcaataaa aatcaaaaag
    23101 caaactagaa agctaggcac agactgctca aaacaccgtt ttaaggttgt ggatagaact
    23161 gacgaagtca gctcaaaaca ctgttttgag gttgtggata gaactgacga agtcagtaac
    23221 catacccatg acaaaccgac gctgacgtgg tttgaagaaa tttttgaaga gtatcattca
    23281 ccatttcaca actaaatcga agactttctg ccgtaatgaa aacacctgaa acagcttggt
    23341 ttcagctgtc cggaaacttt aagacttagg ttcaaagttt aggtatggaa cttcgaagga
    23401 ggtcgctacc gtccgtcatt acttaggaaa gtcttaaaaa atctataaag taaaaagagc
    23461 ccgacagctc tttttgttta tactcaatga aaatcaaaaa gcaaactagg aaactagcca
    23521 cgggctgctc aaaacactgt tttgaggttg tagatacgac tcacatatat acggcaagcc
    23581 gacgctgacg tggtttgaag agattttcga agagtattat tctccatcaa aggcatcttt
    23641 aatatggtca aagaagcctt ttttctttgg atttactttc aagtcaccag cagccgcgaa
    23701 ttctttcaag gctacttttt ggcggtcgtt caagcctgtc ggtgttacga cattaacagt
    23761 aacgtattgg tcaccaactg caccgccacg aaggctcggt gcccccttac tacgtaggcg
    23821 gaatttctta ccagtctgag ttccctctgg aataaccaat tcaacatcac cgtgaacagt
    23881 tggaatatct actgtatcac caagagccgc ttggacaaag ttgaggttga gattgtagaa
    23941 gatagtcgtt ccttcacgtt caaacttgtc gctagcttct acagaaacta ctacatacaa
    24001 gtccccataa ggtccaccgt taaagcctgc ttcgccttga ccagcgaggc gaatttgttg
    24061 acctgtttcc acaccagcag ggattttcac atgtacgcta tgagcttgtt tctcatgacc
    24121 tgttccatga caggttgtac atggatattt gatttctttt cctcgaccgt gacagacatc
    24181 acaggttact tggcgacgca tcataccaag aggagtctgc gtatcgacgt taatgacacc
    24241 agcgccatga cagcgtccac aagtgactgg acttgtccct ggcttagcac cagagccatt
    24301 acatgtacga cagccagctt cacgatgata cttaacttcc ttctcagttc cgaagatagc
    24361 ttcttcaaag gtcaaattga cacgatactg gagatcatct ccttggcgag gagcgtttgg
    24421 attgcgcgaa gaaccgcctc cgccgaagaa acttgagaaa atatcctcaa aaccaccgaa
    24481 gccacctgcc ccattgaaac caccagctcc accaaaacca ccattggcgc ctgcagcacc
    24541 atactggtca taggcagcac gtttttggtc gtcactcaaa gtctcatagg cttcttgaac
    24601 ttccttgtac ttgtcctcag caccaggctc cttgttgata tctgggtgat attttttgga
    24661 aagcttacga taagcctttt tgatttcgtc tgccgaagcg tttttggata cccccagacg
    24721 atcataaaat tcagtattgt tcataattca agatacaaag ggcgtcaaac ggacacagcc
    24781 atctaggagt tttgacgacg gacgcttacg tcctagaaaa aactatcttt ttggcacagt
    24841 ccatagcccg tgttcagtta cgaacaccct tattcctttc tattgatgtt ctgaatcgaa
    24901 ccaccattta ggtcgggttc aattcctttc ttttatattt agtaagaaat tctgaaaccc
    24961 gtgttcagtt acgaacactc tttgttcctt tctattgatg agacaaaacc caaacaaatg
    25021 agattccgtc cctttttatc gaaaaacaga ggctgggttg caacctctgc aaataagtaa
    25081 attaacttta ttttgatttt aaatatccta taagtacaga aatatttata tatttttgtc
    25141 tcttcttagt atttgcctat ataattccaa ttgatcataa gaaatacaac tactgtccaa
    25201 atcataccaa ttatgaacgg tattacaaat ttatatgttt gtatttctaa tgatatcata
    25261 gctggtacca tcgccgatac aaatgcagac aacaatactg aggtaacaaa attccttacc
    25321 gcttcatcat ttttgataca actatcatgt tcagctaata taatttctct atatctagct
    25381 aaatattcta ttggaatatt atcaataaaa tcttttgatg ttctatagtt atcagcttga
    25441 ttacaaaaat cattataaac ttttcgtgta ttttttagat actcttcatc caatacactc
    25501 atcttacttt tccgtaaact ctccgtctac gacgtcatcg cctgcgtttc ctgttgcttg
    25561 tgcgccttct gctccttctt gagcttgttg cgctgctgcg gcttgttcgt agagtttaac
    25621 agcaagtcct tgagcttttt cgttcaatgc ttcaagtttt gctttcatgt cgtccaagtt
    25681 gttgtcttct tgagctttct taaggtcatc aagggcagct tgggcagcgt cacgttctgc
    25741 gtcgaagcct ttaccttcag tttccttgat tgtcttttca gtcgcaaaga ttgcttggtc
    25801 tacttcatta cgaaggtcta cttcttcttt acgtttctta tcggcttcag cgtttgcttc
    25861 tgcatctttc atcatgcggt cgatttcttc gtcagtcaaa cctgagttcg attggatgac
    25921 aatagtttgt tctttttgag ttccaaggtc tttggcctta acagacacga taccgttctt
    25981 gtcgatgtca aatgttactt cgatttgagg aattccacga ggtgcagctg ggatatcagt
    26041 caattggaag cgtccaagag tcttgttatc tgctgccatt gggcgttcac cttgaagaac
    26101 gtggatatca acggctggtt ggttgtctgc tgctgttgag aagacttgtg atttagatgt
    26161 tgggattgta gtgttgcgat cgataagttt tgtaaatact ccacccattg tttcgatacc
    26221 aagtgacaat ggcgttacat caagaaggac aacgtccttg acatcaccag taatcacacc
    26281 accttggata gccgcaccca tagcaactac ttcatcaggg tttactgatt tgtttggttc
    26341 tttaccagtt tcagctttaa cagcttcaac aacggcaggg atacgagttg aaccaccaac
    26401 aaggataact tcgtcgattt ctgacaagct caaacctgca tctgaaaggg cttgacgaac
    26461 tggaactttt gtacgttcaa caaggtcacg agtcaaatca tcaaatttcg cacgagtcaa
    26521 agtcatttcc aagtgaagag gtccagcctc acctgcagtg ataaatggca agctgatttg
    26581 tgttgaagtt acaccagaaa ggtctttctt cgctttttca gccgcatctt tcaaacgttg
    26641 cattgccatc ttgtcagtag acaagtcgat accgttttct ttcttgaatt ctgctaccaa
    26701 gtggtcaatg attttttggt caaagtcgtc accaccaagt ttgttgtccc ctgcagttga
    26761 caatacgtcg aagacaccgt cacccaattc aaggatagag acgtcgaatg taccaccacc
    26821 aaggtcaaat accaagattt tttcttcttt gtcagtcttg tccaaaccat aagcaagagc
    26881 tgctgcagtt ggttcgttaa caatacgttc tacttcaaga ccagcaattt taccagcgtc
    26941 ttttgttgct tgacgttgag cgtcgttgaa gtaagctgga actgtgataa cagctttggt
    27001 tactttctca ccaaggtagt cttcagcgta gcctttcaag tattgaagga tcatagctga
    27061 gatttcttgt ggagtgtatt cttttccatt tgcagaaact ttttcagaag ttcccatctt
    27121 agatttgata gagataactg tatctgggtt tgtgactgct tgacgttttg cagcatcacc
    27181 aacgatgatt tctccgtttt tgaatgagac tacagatgga gttgtgcggt ttccttctgg
    27241 gtttgcgatg attttgcttt cagttccttc aagaactgca actgctgagt ttgttgtacc
    27301 taagtcaata ccgataattt tagacatgtg tttttctcct tgttagttaa ttttctattt
    27361 ttctttttga tactcttctt aagtggcgac gccgtcagag cttgacttcg tcaatctctt
    27421 tgactaaact ttgagcctaa ggtctcaaaa tttgcgcaaa tagcgccacg gcgaagagta
    27481 tcgtgtttgg ttcgcttcgc tcactattac aaagccaaac atattttatc tttcttcata
    27541 gtttattgtc gtttcggaca agttttctat tatgtaaatt gcgacacgag gagtcgaaat
    27601 cgattttatt tcgacgacga gttagtaagg aagctaggca aacgccataa cgattgccgt
    27661 tttctgacga gttgctttag ctaccgtcag aattgccgaa tcgaacacgc cctaagctct
    27721 gagtaaaaaa gatagatttt cttgtgttca tcgaacactt cgtcaatctc ctatttttac
    27781 tgcgagcttt ttacgggctt tatatcttag ttatacacca ctaccattgc tgggcgtagg
    27841 atgcggtcat ggagtttgta gcctttttga aagacttgag cgatggtatc tactgggtgt
    27901 tcatcgtctg ctgggagagt ttggatggcc atatggtagt tatggtcaaa ttcgccatct
    27961 gctgcgattt cttcaattcc ttcttctttc aaagcgtgaa tcaagctttc ttgcaccatc
    28021 cccaagccct tcttcacatc atctgtcaaa ccttcaactg caagtgcacg ctcaaggttg
    28081 tcaagagatg gtaagattgc ttttgccaag tcctggctac gataacgttg caagttttga
    28141 cgttcttcat tggcacggcg ttggatattt tgcatttctg catgagcgcg aagatatttg
    28201 ttttcgaact catctgcacg ttcatttgcc aagtccaact cagacttttc aggagttgtt
    28261 tcttcagctg tttccacaac ttcctcttct tgaacttctt ctacttcttc attttttata
    28321 tcttgggcca tgttcgcctc ctttaatgat ttcaatctta atgtacttcg taatgattac
    28381 tgctgaggta gcggtaaaaa tctgtcaact tcatggtcaa aacacggttg accacattga
    28441 cttgattgat tagctgttgg taatccagat taactggacc gataatggct agaattccaa
    28501 ctccccgata aggaatgagg aacttactac taatcaccgc taggtcagct aaacaggact
    28561 cttgaccgtc tgcaacacga acattttgca tctgatcctc acgcaacccc tcacgaatct
    28621 ccaaggccac cttttgcggt tggtcaaaga actgataggc ttctagattg gcaaaattca
    28681 agagatggac cttgcccgcc atcacaatgt tttcgttgaa catttcctta aagatgtgtt
    28741 caaagagatc gatgacatta tccgttgttg taaagtaacg ctggataatc tgcggaatct
    28801 ccgtccgaat cttgtagtga atatctaaaa cggtgtgacc gaggaaacgt tcctgaatga
    28861 tgctcttcag tttcagcaaa tcctcctgca agaagttcct tggaatcaga aactgactag
    28921 taaccgttcg cgactcgtct agggtaaata ccgccaaggc tgtatgttgc cccaaaacaa
    28981 cgatatcaaa ggctgtcaaa cgttgcctgc tcggctcaac atccagtgcc actaccgtac
    29041 agccactcag gtctgttagc aagttagcag cctcttgcag aatatcctcc aatttgaaga
    29101 attcctgatc aaaggctttg acaatctcat atacctcatt ttcagccagc cggtgaaaat
    29161 ccagtgagtg tttcacatag tactgaaaac cagcaacact tggcatccga ccgcttgaag
    29221 tatgagcctt ctcaagcaac ccttgttttt ctagttccgc catgtcatta cgaatggttg
    29281 cactgctaga gttaatagac tcttgcaagg cttttgatcc gacaggttcg tgcgttttgg
    29341 taaagatgtc aataatcaga tttaaaatat cctgctgacg ctctgtaacc atctcatcac
    29401 tcctctctgt ctgatcaatt agcactcaat gcatccaagt gctaacttat gattctatta
    29461 tacaccctta aaggagaatg tcaagacaaa aactcaaaaa attagcactc ttttatcaag
    29521 agtgctaaaa atcaatctga agacctagaa ccttgctatt catctactcg gtatttcctt
    29581 tttagtctga aataaccatg aattagataa gaaatcagaa aagcatataa aacaaaaata
    29641 agaaagaaag acatagaaag atcctctcta aacaaactcg tacaaacaac aagaccacct
    29701 aagaaagtag ttgtacagga acgaagtcta gatcgcataa cttcccattt gagcccctta
    29761 ctcatataga cttgatcctc tggaagtaaa tcagaagacg atttacgaag aatcgaacaa
    29821 gaacctgctc ctatcaattc ccaacctcta tctctaaaat cttgcagttc atgcttatat
    29881 tttttaagaa atctagaatc atagatacgg tagatgacat cgtctggttg acattggtca
    29941 aaatagaaca aaccaaaacg actcgttcta tacctccaac ctttcaaatg catctcatgt
    30001 aaatgttctt cttccttgtc caaatcaaca atggtgaaaa tccgaaattc tactctgcta
    30061 ttcattgtct taccccaaaa ttagaaaaca tgcctggcgt tatttattag ataattcttt
    30121 ccacttttga ctcaatctcc aaaaaatata agaaatctga atcgcaaaaa ctatcaataa
    30181 aacccaatct attatgaaaa tcaaaaacac tttccaactg aaagaactac ctccagtgac
    30241 aaactttgag aaaaacggta gtagagctaa aaagagaagt aaaataggaa gcatccgcat
    30301 tgttaaaatc cgtttggcat aaaaaaatct ttatttaaac taaaatatta tagcaaaatt
    30361 tacgccagtt tttgaacggc tgatgtagat attttatact ttcaaaatgt ttaaatgtga
    30421 ttatttattt ttgaaaaata gctcaccagc ccgactgaaa gtgcttatag aatgataata
    30481 agtcgcctgc cgaaaacagc ggaaaatagc ggtgttatgc ggagataatc tgacgcgatg
    30541 cgaaagtata ttgcatactt attttcaaca atttagcaga gtatttttat aagtgtgata
    30601 taatagaagt ataatttgtt ctgatagttt attttatgga gaagtagatt tttagaatgc
    30661 ggagggttca atatggttga gtttataaag tctaagaaag aaatgagtga ggaggatatt
    30721 aaagcaaatt tcatcactcc tgctattgta tccaaaggat ggaaaaatgg tgagcatatc
    30781 gcttacgaag aatacttcac tgatggtcga attgaggtta gaggagataa ggctcgtcgt
    30841 aaagaaggaa aaaaatcaga ctattcactg tattaccaat ttggaactcg aattgcaatt
    30901 gttgaggcaa aggataataa acacagcgtt cgagcaggat tacaacaagc tattgaatat
    30961 ggagagattt tagatgttcc atttgtttat tcttcgaatg gtgatggctt tattgaacac
    31021 gaccgtatca cgagagaaga acgtgagctg gagttagacg aattccctac tcgtgaagaa
    31081 ttattttctc gtatgacgaa ggaaaaagga ttgacgtacg aaattacaga agctatctca
    31141 actccatact atacagacgc cttctcaatg aaaacgccac gctattatca gcaaatagct
    31201 atcaaccgta ctattgaaac agttgccaga ggacaaaaac gagtaatgtt tgtgatggca
    31261 acaggaacgg ggaaaacgtt catggctttt caaattattc atcgccttcg aaaagctggt
    31321 ttggctaaac gagttttatt cttagcggat agaaacatct tagtagacca aacgatggct
    31381 gaagacttta agccattcga aaaggtaatg acgaaaatta caccaaaact tttgactgct
    31441 cctgaaaaat taaattcttt tgaaatttat ctagggcttt atcagcaact aactggtgaa
    31501 gatggaactg aaacacatta tcaaaaattt gacaaagact tctttgattt aatcgtaatt
    31561 gatgaagcgc accgtggttc agctaaggaa aacagtaact ggcgtaagat aattgattat
    31621 ttcagttctg cgacacagat tgggatgacc gctactccta aagaaaccaa gaatgcttcc
    31681 aatacggaat actttggtga gccaatctat acttatagtt taaaacaggg aatcgaggat
    31741 ggttttttgg ctccatatcg tgttatgagg gttaatttag atgtggatgt ggatggttat
    31801 cgtccagaaa ctggaaaagt tgatgctaac ggacaattaa tagaagatag gtactacagc
    31861 aggaaagatt ttgataaaac cattgtcatt gatgatagaa cgcaaagagt tgccaagttt
    31921 gtttctgatt atatgaagca aaacaatgca cgatttgata aaacaattgt tttttgtgtt
    31981 gatattgacc atgccgagcg aatgcgtgct gcacttgtaa aagagaatct agacttagtc
    32041 caagaagact atcgttatgt catgcaagta actggtgaca acgctgaagg aaaagctcaa
    32101 ctggataact ttatggatgt caattctaat tttcccgcta ttgtaacaac gtctaaatta
    32161 ttaacgacag gagttaatgc taaaacatgt cgtttgattg ttttagactc taatatccaa
    32221 tccatgactg aatttaaaca aattattggt cgtggcacac gtctttatcc tcaaaagggg
    32281 aaagaatttt ttacgattat tgattttcga aatgttacca atttgtttgc tgaccctgat
    32341 tttgatggtg agccagtgaa ggtgctggaa acaggtgcga aaacagtcag tggttctacg
    32401 cccggtttcg tagatgagga aggtgaccca gtagaaaaat atatcgttac agacaagcag
    32461 gttaccattc ttaattctac tgttcaagta ttggatgaaa acgggaaact gattaccgaa
    32521 agcctgaccg actacactcg gaagaatatc ttaggtagct acgccacttt gaacgatttt
    32581 atcacagttt ggcatacggc ggataaaaag aagcttatct tagacgaact ttataaaaaa
    32641 ggagtttatc tagatgctat tcgagagtcg gagggaatat cagaacaaga aatcgatgat
    32701 tttgatttac tcctaaaact tgcctatggt caaaaagaat taaccaaaac ggaacgtatc
    32761 aataaactca aacaaagcgg atatttatat aaatatagtg aggaagcgcg tgctgttttg
    32821 gaaattttac tgaacaaata catggataaa ggtattggag aactcgaaag cattgaaaca
    32881 ttaaaacttc cagaatttca gatatatggt ggaaccttca aaatcatcaa tacttatttt
    32941 ggagataaaa aacgatattt acaagcaatt aaagaattgg agcaagagct atttacagta
    33001 gcttaatgaa aggaaagtat gtcaattaca tcatttgtaa aaagaattca agatatcact
    33061 cgaaacgatg ctggtgttaa tggtgatgct caacgtattg agcaaatgtc ttggttatta
    33121 ttcttaaaaa tttatgatag ccgtgaaatg gtttgggaat tagaagaaga cgagtatgag
    33181 tcaattatcc cagaggaatt aaaatggcga aattgggctc atgctcaaaa tggggaacgg
    33241 gtattgacag gcgatgaatt acttgatttt gtcaataaca agttattcaa agagttgaaa
    33301 gagcttgaaa taacttcaaa tatgcctatt cgaaaaacga ttgttaaatc agcttttgaa
    33361 gatgcgaaca actatatgaa aaatggcgtc ttgttacgcc aagtcatcaa tgttattgat
    33421 gaagttgatt tcaatagccc tgaagatcgt cattcgttta atgatattta cgaaaaaatt
    33481 cttaaagata ttcaaaatgc tgggaactca ggagaatttt atacgccacg tgcagcgact
    33541 gattttattg ccgaagttct tgacccaaaa cttggagaat caatggtaga ccttgcttgc
    33601 ggaacaggag gcttcttgac ttcgactctg aaccgtttaa gtagtcaacg taaaactagt
    33661 gaagatacca aaaaatataa tacagctgtt tttggtattg aaaagaaagc atttcctcat
    33721 cttttagcag ttacaaatct gtttcttcac gaaattgatg accctaaaat tgttcatgga
    33781 aatactttgg aaaaaaatgt tcgtgaatat acggatgatg aaaaatttga cattattatg
    33841 atgaatccac cttttggagg ggcagaatta gaaacaataa aaaataactt tccagcagaa
    33901 ttacggagtt ctgaaacagc tgatttattt atggctgtca ttatgtatcg tttgaaagaa
    33961 aatggtcgtg ttggagttat tttacctgat ggttttctat ttggtgaagg tgtaaaaact
    34021 cgcttgaaac aaaaactggt agatgagttc aacttgcata cgattattag gttgcctcat
    34081 agtgtctttg caccgtatac aggaatccat acgaacattc ttttctttga taaaacaaag
    34141 aaaacagaag aaacttggtt ttatcgttta gatatgccag atggttataa aaatttctcg
    34201 aaaactaagc cgatgaagtc agaacacttc aatcctgttc gtgactggtg ggaaaatcgt
    34261 gaagagattc tggaaggtaa gttctacaaa tctaaatcat ttacacctag tgaattggct
    34321 gagttgaatt ataatttaga ccagtgtggc tttccaaaag aggaagagga aatcttaaat
    34381 ccctttgagt tgattcagaa ttatcaagcg gaaagagcaa ctttaaatca taagattgat
    34441 aatgtattag ctgatatttt gcagttgttg gaggacaaat aatgacacca gaacaactta
    34501 aagcaagtat tctccaaaga gcgatggaag ggaaattagt gccgcaaaat cccaatgacg
    34561 aacctgcaag tgaattatta aagagaatta aagctgaaaa agaaaaactt atcagtgaag
    34621 gaaaaatcaa acgagataaa aaggaaactg agatatttcg tggtgatgat gggaaacatt
    34681 atgggaagtt tgctgatgga agcactcaag aaattgatgt tccttatgat attcctgata
    34741 cttgggagtg ggtgaggttt tctacattgg ttgaaattgt cagaggtggc tctccacgac
    34801 caatcaaaga ttatcttact tctgaagtag atggaataaa ttggataaaa ataggtgata
    34861 ctgaaaaggg tgaaaagtat ataaataatg ttaaagaaaa aatcaaaaaa tcaggactta
    34921 acaaaactag atttgtaaaa aaaggtacat ttttgttaac taattctatg agttttggta
    34981 gaccttatat tttgaatgtt gatggtgcaa tacacgatgg atggttggct atttcgaact
    35041 atgaaaactc attaaataaa gattacctat tctatattct ttcatcaaat gtagtttatt
    35101 ctcaatttct atctctaatt agtggagctg ttgtgaaaaa cttgaatagt gataaagttg
    35161 cttctattct tatccctctc cccccactat ccgaacaaca acgcataata gaagcaatcg
    35221 aatcagcttt agaaaaagta gatgaatatg ctgaaagtta taatagacta gaacagctag
    35281 ataaagaatt tccagataaa ctaaaaaaat ctattcttca atatgctatg caaggaaaat
    35341 tagttgaaca agacccaaat gatgaatcag tcgaagtttt acttgaaaaa atacgagcag
    35401 aaaaacaaaa actctttgaa gaaggcaaga ttaaaaagaa agatttggac atttctattg
    35461 tttcccaag
//
LOCUS       DBERMR010000016        34614 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.016, whole genome shotgun sequence.
ACCESSION   DBERMR010000016 DBERMR010000000
VERSION     DBERMR010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 34614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34614
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.016"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(502..1674)
                     /locus_tag="ACL8A1_000873"
                     /pseudo
     CDS             complement(502..1674)
                     /locus_tag="ACL8A1_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013730289.1,RefSeq:WP_050084071.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1655..1849)
                     /locus_tag="ACL8A1_000874"
     CDS             complement(1655..1849)
                     /locus_tag="ACL8A1_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGS1201668.1"
                     /translation="MKIIQQQSAIIDSLTNELSLLCEQVAYLTQKLSGKSSEKSVCPF
                     GQLNLFEEESPSEKDGDVPS"
     gene            complement(2019..2369)
                     /gene="tnpB"
                     /locus_tag="ACL8A1_000875"
     CDS             complement(2019..2369)
                     /gene="tnpB"
                     /locus_tag="ACL8A1_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGS1201669.1"
                     /translation="MTIHLSSLGQVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWLLYKRFENGRLTWPSTEKDVKALTPEQVDWLMKGFS
                     ITPKINLSESRDFY"
     gene            complement(2350..2535)
                     /locus_tag="ACL8A1_000876"
     CDS             complement(2350..2535)
                     /locus_tag="ACL8A1_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201670.1"
                     /translation="MPQPIVPVEIPQSRPFDSKKRNDILLKIRIGKLEVSFFQSLNLE
                     MVEQLLDKVLLYDNSSI"
     gene            complement(2839..4401)
                     /gene="guaA"
                     /locus_tag="ACL8A1_000877"
     CDS             complement(2839..4401)
                     /gene="guaA"
                     /locus_tag="ACL8A1_000877"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HGS1201671.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            4543..5241
                     /locus_tag="ACL8A1_000878"
     CDS             4543..5241
                     /locus_tag="ACL8A1_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGS1201672.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQISYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(5284..6180)
                     /locus_tag="ACL8A1_000879"
     CDS             complement(5284..6180)
                     /locus_tag="ACL8A1_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGS1201673.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAIFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(6189..7124)
                     /locus_tag="ACL8A1_000880"
     CDS             complement(6189..7124)
                     /locus_tag="ACL8A1_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HGS1201674.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(7121..7846)
                     /locus_tag="ACL8A1_000881"
     CDS             complement(7121..7846)
                     /locus_tag="ACL8A1_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HGS1201675.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDIASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(7930..8565)
                     /locus_tag="ACL8A1_000882"
     CDS             complement(7930..8565)
                     /locus_tag="ACL8A1_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HGS1201676.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEREEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKSLKDYLY"
     gene            complement(8567..9394)
                     /locus_tag="ACL8A1_000883"
     CDS             complement(8567..9394)
                     /locus_tag="ACL8A1_000883"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGS1201677.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALTHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(9416..9751)
                     /locus_tag="ACL8A1_000884"
     CDS             complement(9416..9751)
                     /locus_tag="ACL8A1_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HGS1201678.1"
                     /translation="MGNFISNQRIESMGDEENAKWTERGVLMDVTIKKKDGKTTIGTA
                     KAHPTWVNRTPKGTFSPEGYPLYHYQTYILEDFIEDGSHRDQLDEATKERIDTAYKEM
                     NEHVGLKWY"
     gene            complement(9752..10342)
                     /locus_tag="ACL8A1_000885"
     CDS             complement(9752..10342)
                     /locus_tag="ACL8A1_000885"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HGS1201679.1"
                     /translation="MGDFEGTINKDHYLAGYLLFNAPVEVMDAIKEAGYHVLDLAHNH
                     ILDSQIEGVISTADIIEKAGITPIGVYTHEPRDQAPLVIKEVNGIKVALLAYSYGFNG
                     IEQYISQEDYNRYLSDLNEDKMKVEIERAEKEADITIIMLQMGVEYRLEPTEEQKALY
                     HKMIDLGADIIFGGHPHVVEPSETVEKDGDKKLIIY"
     gene            complement(10358..10480)
                     /locus_tag="ACL8A1_000886"
     CDS             complement(10358..10480)
                     /locus_tag="ACL8A1_000886"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201680.1"
                     /translation="MAHGDLLYHDGLFFSAKKEDGTYDFHENFEYVTPWLKQGD"
     gene            10993..11604
                     /gene="def"
                     /locus_tag="ACL8A1_000887"
     CDS             10993..11604
                     /gene="def"
                     /locus_tag="ACL8A1_000887"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HGS1201681.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRTVAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            11649..12377
                     /gene="rlmB"
                     /locus_tag="ACL8A1_000888"
     CDS             11649..12377
                     /gene="rlmB"
                     /locus_tag="ACL8A1_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HGS1201682.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(12416..13327)
                     /gene="trxB"
                     /locus_tag="ACL8A1_000889"
     CDS             complement(12416..13327)
                     /gene="trxB"
                     /locus_tag="ACL8A1_000889"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HGS1201683.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLIVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDSHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(13393..13617)
                     /locus_tag="ACL8A1_000890"
     CDS             complement(13393..13617)
                     /locus_tag="ACL8A1_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HGS1201684.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(13695..14438)
                     /locus_tag="ACL8A1_000891"
     CDS             complement(13695..14438)
                     /locus_tag="ACL8A1_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGS1201685.1"
                     /translation="MIKISNLSKSFSGQTVLDHLNLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMPFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(14438..15238)
                     /locus_tag="ACL8A1_000892"
     CDS             complement(14438..15238)
                     /locus_tag="ACL8A1_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGS1201686.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(15396..15752)
                     /locus_tag="ACL8A1_000893"
     CDS             complement(15396..15752)
                     /locus_tag="ACL8A1_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HGS1201687.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(15746..16276)
                     /locus_tag="ACL8A1_000894"
     CDS             complement(15746..16276)
                     /locus_tag="ACL8A1_000894"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HGS1201688.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDRSNRRRSTC"
     gene            complement(16276..16770)
                     /locus_tag="ACL8A1_000895"
     CDS             complement(16276..16770)
                     /locus_tag="ACL8A1_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HGS1201689.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            16905..17351
                     /locus_tag="ACL8A1_000896"
     CDS             16905..17351
                     /locus_tag="ACL8A1_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HGS1201690.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTIKLYHQTLDLIDHTQEEVIQ"
     gene            17348..17995
                     /locus_tag="ACL8A1_000897"
     CDS             17348..17995
                     /locus_tag="ACL8A1_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HGS1201691.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASVLQYIAIVYYM"
     gene            complement(18016..18597)
                     /gene="pdxT"
                     /locus_tag="ACL8A1_000898"
     CDS             complement(18016..18597)
                     /gene="pdxT"
                     /locus_tag="ACL8A1_000898"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HGS1201692.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(18598..19473)
                     /gene="pdxS"
                     /locus_tag="ACL8A1_000899"
     CDS             complement(18598..19473)
                     /gene="pdxS"
                     /locus_tag="ACL8A1_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042819 - vitamin B6 biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HGS1201693.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(19625..21004)
                     /gene="nox"
                     /locus_tag="ACL8A1_000900"
     CDS             complement(19625..21004)
                     /gene="nox"
                     /locus_tag="ACL8A1_000900"
                     /EC_number="1.6.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H2O-forming NADH oxidase"
                     /protein_id="HGS1201694.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVITAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            21298..22221
                     /locus_tag="ACL8A1_000901"
     CDS             21298..22221
                     /locus_tag="ACL8A1_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HGS1201695.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            22281..22886
                     /locus_tag="ACL8A1_000902"
     CDS             22281..22886
                     /locus_tag="ACL8A1_000902"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HGS1201696.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKTIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            22904..24148
                     /locus_tag="ACL8A1_000903"
     CDS             22904..24148
                     /locus_tag="ACL8A1_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HGS1201697.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRNFKFKFELEVLEIDEVPMF
                     NQDEKWDESFQLRFLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNHGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGDHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(24371..24628)
                     /locus_tag="ACL8A1_000904"
     CDS             complement(24371..24628)
                     /locus_tag="ACL8A1_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HGS1201698.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(24670..26706)
                     /gene="glyS"
                     /locus_tag="ACL8A1_000905"
     CDS             complement(24670..26706)
                     /gene="glyS"
                     /locus_tag="ACL8A1_000905"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HGS1201699.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQSVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEEEFDLDFLDIKGGRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(26966..27883)
                     /gene="glyQ"
                     /locus_tag="ACL8A1_000906"
     CDS             complement(26966..27883)
                     /gene="glyQ"
                     /locus_tag="ACL8A1_000906"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HGS1201700.1"
                     /translation="MSKKLTFQEIILTLQRFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRVKLLAEDAE"
     gene            complement(28077..28838)
                     /locus_tag="ACL8A1_000907"
     CDS             complement(28077..28838)
                     /locus_tag="ACL8A1_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HGS1201701.1"
                     /translation="MENLYLVKDDSQLAAFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSNFNAATQIIRESAVPAYTNNRRMVMTPDLAVWKELYLYQLMDYECSQQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVLDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            complement(29111..29953)
                     /locus_tag="ACL8A1_000908"
     CDS             complement(29111..29953)
                     /locus_tag="ACL8A1_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HGS1201702.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQDQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     VAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTIAVQSGAPRVD
                     DVDF"
     gene            complement(30067..31458)
                     /gene="pgdA"
                     /locus_tag="ACL8A1_000909"
     CDS             complement(30067..31458)
                     /gene="pgdA"
                     /locus_tag="ACL8A1_000909"
                     /EC_number="3.5.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050119 - N-acetylglucosamine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-N-acetylglucosamine deacetylase
                     PgdA"
                     /protein_id="HGS1201703.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKHQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(31696..32064)
                     /locus_tag="ACL8A1_000910"
                     /pseudo
     CDS             complement(31696..32064)
                     /locus_tag="ACL8A1_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603324.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
     gene            32285..33262
                     /locus_tag="ACL8A1_000911"
     CDS             32285..33262
                     /locus_tag="ACL8A1_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGS1201704.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFEKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            33259..34341
                     /locus_tag="ACL8A1_000912"
     CDS             33259..34341
                     /locus_tag="ACL8A1_000912"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGS1201705.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTINLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILTVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(34405..34614)
                     /locus_tag="ACL8A1_000913"
                     /pseudo
     CDS             complement(34405..34614)
                     /locus_tag="ACL8A1_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT        10282 a         7708 c         6212 g        10412 t
ORIGIN      
        1 taccataatt aaagagatga gagataaata tcggcagtct tcggactgcc tttatttttt
       61 tacttaattt aatgcagtaa atcatacgat tgaaaggaat ctgcgttata tgctataatt
      121 aggtagttaa tctatacatt tagaaaggta aaatatagaa ttggagaatg atatgggatt
      181 ttcatataat aaattgtaac cgcccaataa cgaagtctat tgaaaaatct ccagattagt
      241 gaactcacgg atagttccta atctggagat ttcttatttg cacttttctt gtacaacttt
      301 agtccacggt aaataggcct ctaaaacctc tttgtttacg agagtttcca cgtttggaag
      361 acattctaga agataggata gatatttctc actatttata atggattgaa ataagatatg
      421 aacaaatcga ttaggaaagg aaaattgatt tatagaaatg ttttagcagt cgatgcgtac
      481 tgttatagat tcaacgcact ataattgatg acgtttagct gtttccaaca aactcataat
      541 aatagctctt gctctagctc cttcaaaact ttgagaaaat aaccaccctc gcttttttaa
      601 actgagctta ggctaaaaga gtccactgga ctcttttact ccgtcccata accaatgatt
      661 taatggcgca ttcagctaga ttattggaaa ggaccagatg tccgtctttc aaaatagtct
      721 taaaggtttc tttatacttg aggctatact tgaggctgta ttcaattgcc cttcctagtt
      781 ttgaacccga taaaactgac tgacgccggc accaagcaaa gaaatcttcc attaggggtt
      841 ggagctcttc ttgacgtttc tgtagtcgtt catcagctgg caaagcctcc cagtttcttt
      901 ccaaggaaaa taactgatta caataagcta aacctttagc tcctaaggat gatttattcg
      961 cttgcttggg ggtcgcttca aaaaacttcc ttctcacatg tacccaacat ccaacaagtt
     1021 cagcttcttc cagttgacaa taagcttcta gcggttcgca gtttaccaag cttagcgtcg
     1081 ccttgctcct aaaccttgga ctgctatcgc ataggcagaa ctagaggact aaagtcctaa
     1141 gttactgccg caacatatca caatgcacat agccagaata atctcctagg aattcttgca
     1201 ctaccgaacc actccgacgc tgatcatggt ggtacagcgt gattgcttga ttctcagctt
     1261 tcccagacaa aaaggtccaa tagtaggtca gatggctatc attctctaga acccgatagg
     1321 aggtttcatc tgcatgaagt aagggctgaa tcaatagtct cgttataaag gggctttagg
     1381 aataggagct tttacaattt tatcacttgg attattctta ctgcatgttt ggcatttgta
     1441 agcgtgttgg atatgatcca ctctttttaa ttgcgcagga ataaagacca attcttgtcg
     1501 ttgaaggctc gctccaatct cttttaaatc gtcctgacaa tcagggcaaa tgctctcttc
     1561 tacttgatga tgaacttctt ctgaatcaaa ttgggcaaga atagcttgac gtttcccttt
     1621 agatttctta cgtttgtagg taatttcctc tgtttcaact gggaacatct ccatctttct
     1681 cagatggaga ctcttcctca aaaaggttga gttgtccaaa agggcaaaca cttttctcag
     1741 aggattttcc agagagcttt tgcgttagat aagccacttg ttcacacaga agggaaagtt
     1801 cattagtgag actatcaatt atagcactct gttgttgaat gattttcaat cttctcccat
     1861 agactttcct ccttatttcc taactttatt atatagtgta ttgaatctat aacagtacac
     1921 cttgactgct aaaatatttc tataaattaa tttgactttc ctgatagaga tgttcatatc
     1981 ttatttcaat tcactatact aaaaagaaag tcctcatttc aatagaaatc acgactttct
     2041 gataaattta ttttgggagt gatagaaaag cccttcataa gccaatctac ttgttcaggt
     2101 gtgagagctt tgacatcctt ttctgtactg ggccaagtca gtctgccgtt ctcaaagcgt
     2161 ttatatagta gccaaaatcc ttgaccatcc cagtaaaggg ctttaaagcg gtctttacgt
     2221 ccaccacaaa agagaaaaac ttgaccggag aaaggatcca attcaaagtg ggttttaacc
     2281 agataagcca gtgaatcgat tccttgtctc atatcagtct tcccacacac gagatagacc
     2341 tgccctaggc tagatagatg aattgtcata gagcaacacc ttatccaaaa gctgttctac
     2401 catttcgaga ttgagagatt gaaaaaaact tacttcaagc ttgccaatac gaattttaag
     2461 cagaatatca tttctctttt tagaatcaaa aggacgagat tgtggaatct ctacaggaac
     2521 aataggttgt ggcattaaaa tatcctccaa tagttttact ttactaatag tatacaggag
     2581 gaactccatg aatgatagat accttgttat gacgcgctta ctatattaga aagttggctc
     2641 tctaattaaa ttaattctca ttatatatat caaaagaccg cagtttgaaa actacggtct
     2701 aattttttgt ccaaatgtaa atcacttgga actattattc aatttattct ttttagtaaa
     2761 tgttcccgtt ttaatcccag ttacagtttt taatcctctg taaccgttga ttttacaaag
     2821 cttttaattt ttttgcgatt actcccactc aactgttgca ggtggtttac ttgtaatatc
     2881 gtagacgata cggttaacat gatccacttc atttacgata cgtactgaga ttttttgaag
     2941 tacttcccat ggaattttgg caaaatcagc agtcatacca tcgatagaag tgatagcacg
     3001 gattgcaatc gtgtagtcat acgtacgacc gtcacccata acaccgactg aacgaacgcc
     3061 tgtgttaaca gtgaagtatt gccaaatatc gcggtcaagt ccagctttag cgatttcttc
     3121 acgaagaata gcgtctgatt cacgaacggt ttcaagtttc tcttcagtga tttcacccat
     3181 gacacggata gcaagtcctg gtcctgggaa tggttggcgc catacgatat ggtctggcat
     3241 accaagctct gtaccaagag cacgaacttc atccttgtaa agagtattga gtggttcaat
     3301 caattcaaac tgcatatctt ctggaagacc acccacgttg tggtgtgact tgatagtttg
     3361 agctgtatcc gtaccagact cgataacatc tgtatataaa gtaccttgag caaggaattt
     3421 cacatctttg agcttgcttg cttcgtcatc gaatacatag acaaactcgt taccaatgat
     3481 tttacgtttt tgttcaggat cagaaacgcc agcaagtttg tcaaggaaac gtttagcagc
     3541 gtctgctttg acgatattca aaccaaactt accaccgagc atgtccataa cttgatcagc
     3601 ttcgccttta cgaagaagac cgtggtctac gaagatacag atcaattgat cgccaatcgc
     3661 tttttggaga agaaccccaa cgacagatga gtcaacacca cctgatagac caagaaggac
     3721 acgtttatca ccgacggttt cacgaatttt tttgatctgc atgtcaatga aattatccat
     3781 tgaccagtca cctttagcct tacaaatgtt aagggcaaag ttacgaagga tatcatttcc
     3841 gtatacagaa tgacgaactt ctgggtggaa ttggataccg taaatgtgtt tatctgggtt
     3901 ttcgatggct gcgtatgggc agtcagctga tgtacctgta cgaacaaagt cagcaggaat
     3961 ctcagtaacc gcatcaccat ggctcatcaa aacagtctgt tcatcaggtg ttgattcaaa
     4021 aagcgctgat ggtgtgtgag ttagggttga ttgaccgtat tcacgatttc cagcatcacc
     4081 tgcaggaaca acttttcctc caagtttatg ggtcaataac tgcataccat aacagattcc
     4141 caaaattgga attccgagtt cgaagatttc tgggtcaata tcaaatgaac catcttcata
     4201 tacagaattt ggaccacctg atagaataat tcctacagga ttgacttcac gaacttcagc
     4261 agctgaaatt ttatggcttt ttagttctga aaaaacacca atctcacgga tacggcgtga
     4321 aatcagctgg ttgtactggc taccatagtc caatacgatg attttttcta catcttgcaa
     4381 atcagttgaa atgttgctca tctttttcct ttctcaaaag aaatatcttt tctcaatatt
     4441 ctatcacaaa taagggcgaa ttaccaacta aacaaggcga agtttgactt tttctagttt
     4501 attggggtac aatagagaaa agatagaaat gaagtgaaaa atatgctacc tgcttatatg
     4561 aaaatccatg atcagataaa aaaggatatt gacgagcacc gttgggctat tggtgagagg
     4621 cttcccagtg aaagagattt agctgagcag tttgcggtca gtcgcatgac cctccgccaa
     4681 gccgtatctc tattagtcga agaaggcgtc ttagagcgcc gtgtaggaag cggcaccttt
     4741 gtttccagta ctcgagtaca agaaaagatg cgagggacaa ccagttttac tgaaattgtc
     4801 aaatcccaag gtaaagttcc ctctagccag ctcatttcct acaaaaaaac cattcccaat
     4861 gagcaagaag ttgccaagct aggaattttt ccaacggaca atattatccg aatggagcgg
     4921 gtccgctatg ccgaccaagt tcccctagtt tatgaagttg cttctattcc tgaaaaattc
     4981 attaaggact ttaaaaaaga agaaatcacc agtcatttct tccaaacctt gcaaaaacat
     5041 ggctatcgta tcggtaaatc ccaacagacc atctatgctc gccttgctaa agaaaagatt
     5101 gcccactatt tggaagttga aaaaggacat gctattcttg gattgaccca gatttcctac
     5161 ctagaagatg gtactgcttt tgaatacgta aaaagtcaat atgtaggcga acgctttgaa
     5221 ttttatcttg aaaataatta gattaccacg caaaaactct ctgtcacgga caaagagtct
     5281 tttttatttt tctaagaata aatctttcaa agaaatcaaa gttacagcaa acaatatcat
     5341 tgccatacca agaaaatcaa tgggataaaa ttgttcattc attattaaga aggcaaagaa
     5401 aatcgccgat attggctcaa ttgaagccaa caagcttgac ttgaccggtc ctatcagact
     5461 ggctccttta aggaaagctg tataggcaaa gacagtcccg ataaggataa tgcctgcaaa
     5521 cgcaaggaga aaatcaagac tagtcgggat atcggcctgt agaacccctg taaaaggaag
     5581 ggcgaccaaa cctgctatga ccattcccac accaatgacc aagctgctcc cccacttttt
     5641 aatcaaggct atgggtaaaa tgatatacag agcataagtc aaggcagaaa agagacccca
     5701 gaacagacca gcaggtgtca tggataactg gtccaactgc ccatgtgttg cgatcaggaa
     5761 ggttcctccg atggcgaata tgatggaaac tatctctccc agtgtcggtg ccaccctatc
     5821 cttgatacag ctataaatta aaattccgac aggacaaaca tactgaagca ccgtcgctgt
     5881 tcccgcattg gtctcctgaa tagcagacag ataggcgaat tggttgagaa aaagaccaat
     5941 cagagcaaaa ataagaagag acagcaaact ctttctatcc tttaaaaaga ccagtatttt
     6001 atcctttgca gtagcataag ccaagagcat gagaattcca ccagcgatta aaagacgcaa
     6061 gttagtcaag accagagccg aaattccgtg tgccattagg tattggccac tcgttcctga
     6121 caacccccaa gcaataccag ccacaactgt tagtaaagtc ccttttaaac tatttgacat
     6181 gtctctcctt actcttcttt gcgaatcaag ttcatcactt gattgcggtc taatatccat
     6241 tgagctctct cgtccttttc ataggtacga ttcgatagga aaatagtggc ttcttgcttc
     6301 tgacgattcc acatgataaa ggtacctgta tagcccgtat ggtctagcca atctccttcc
     6361 aaattccatg ctaaagaacg ttccttgtca tccaaaggag aaaaattttg atttaagtct
     6421 cttgcaaaat catctgctaa atagtgttct aaaaagattt gtaaatcctt tatagtcgaa
     6481 aataaaccag cactcccagc atgtctaccc aggagacgag ccttgggatc atgcactatg
     6541 cctgcctcta cacctctaac tgttggaaca gcaagctcaa ctggcccaaa cttggtttcc
     6601 gtcattcccc aaggtttcca gacttgatcc tttaaaatca catccaaatc ttgattaaaa
     6661 attctttcca aaataaagcc caacagcaaa aaatggacat ccgaataaag aaaggctggc
     6721 tgacttcgtc tgttgagatg aaacatcgct tcctttaatt caggggctgt taaaagatca
     6781 cgattaggaa taaaaggatc aaggtctgtt gcatgagtca agagctggcg aatagtgatg
     6841 tctggataat cactctcagg taaaaaatct attaccagtc tatcaatatc taattgacct
     6901 atttcccaca agaaggtaca aactgtgcca accccaacaa ccttgctgac actagctagg
     6961 tcataaacta gtcctgcctc agtctgcaag ccatgctctg ggtcactctg gcctaaatag
     7021 aactctgtcc attgattgtc cttaaaatac gcaaaagagg ctccgggata aatccctgcc
     7081 tcgatttgtt cttctatttt tttaataatc ttggtccact tcatacttct tcaaaccaca
     7141 attcaacatt atttctatct ttaccaagga agaatttttc agacttagga ataaaatatt
     7201 cagtagactc aaacttctgg cgaagacttg ctatgtctaa ttcattgacc aagaacttga
     7261 gcatagataa gtcccaggta accgtattgt ccacagtcaa atcctgcccc tgagctggaa
     7321 taaaatcaag ggatgcccca atttcagatg attccaagaa actttcgata tcagttggga
     7381 gatgtaattc catagaaatc tcaaatttac ttaaagaaat tgatgccaaa tctgtttgaa
     7441 attcaggctt ttctcctact tctactagac ttgctatgtc atcttccgca tgaatcaaaa
     7501 tcaaatcatc ttctggtgag aaaatttcaa aagcgtagcc attttgacct ttatataatc
     7561 gatgaatcga atctgtttta gataagattc cttcaatttc taagggattt tccaccttga
     7621 caatcaatct agctagtttt tttcttccct ctaccttacg agtacgcata ctgggagctt
     7681 cttctaaaac cagcttttca agacccgttt ggtcacctag tgacagaaag gccgattctt
     7741 ctaacaaggc cttcattcca agggtttcaa tataaaatgt ttcatttaat tttctattat
     7801 tagcttttaa agtaggaata atccgtacaa tctgatttac attcataaat tcctccgtca
     7861 ctttttattt taaaggattt tagaattttt tacaagatat tatgctcaaa tttaacatta
     7921 tttagctatt tagtaaagat agtctttcaa ggattttgac aaagcctgat aaccagcaat
     7981 actgaggtgc agtccatcag ttgtatattc ttttttgagt tggcctgctt ggtctgtcaa
     8041 acaatcaaat actggcacaa attccacctg catataggca gatgcaagct cttgataggc
     8101 ttgattccag ttctgaattt tttcattcga gcggatatag actgcctgct ggtactcctc
     8161 tctctcattg acaggcaaaa tggaaagcaa tttaatctct gtcaatggat aatcgcgagc
     8221 aacggattga atgatagctt cgagattatt gagagcctca ttcacaggaa catcctttcc
     8281 gatatcattt gtcccaatca gaagaaaaat tttatctact gctccaccat atagatgagc
     8341 atcaaggttc tctagtaaca gtcctgtctg atagccacga attcctcgat tgacaatcgt
     8401 ctttgaagtc ccaaatagct cctgtagagg ataatactcg acaatggaat ccccaataaa
     8461 aagaatgttt ggttctacaa cagaaatgtg atttaggtga cgatacttag tttgaatttt
     8521 ttcttgttcc tttaggagcc aattttctaa taactgtact gccacttcat tctccttttt
     8581 ctaaccagtt ttccaagact tgataaactc ctccttggct gttggctggt gctagagcag
     8641 tcgccacagc tttggctttc tcatcagcat tttccatcgc ataggcaatt ccagccattt
     8701 caagcatttc aacatcattt tcactatcac caaaagccat gatttcttgg gatttcaagt
     8761 cccagcgctt gagtaattcc tccaagcccc atgctttatg aatcccagct tggaggatat
     8821 caatgcaacc atagccactg gatacagctc ggacacggcc atcaaagaga gcattgattt
     8881 cttccaaaac cgaactcaaa cgttcctcac caacaaccat gctcatcttg agcacaccac
     8941 caaagaggtc agatgttaat tcatccacaa attgcatccg ttggtagaat ttttcaatca
     9001 tttctggagt cataaaactt tcaagatctg taaaaatcgt accttccttg acaaaatcac
     9061 ccttcatccc cgttacaaca aactggtcct gacacgctcg acccttgaaa tgagtcaaag
     9121 ccttgttgac aatggcgtca tcccatgtct gagcctgaat caattcattg ttttcaaaaa
     9181 tacgagcgcc attagcaaca accagaacca ctcgatccac caagggactc agtagttgtc
     9241 tcatgcggtg aatttcattg cccgtcgcaa tgacaaaacg aatgcccctt tgatccaact
     9301 gatctaaaat cttttccaat cgtgggagat caagctgacc tctagcatcc agcaaggtcc
     9361 catccatatc tgtcgcaatt accttaatca tcatttactt tcctctggat tcaagctaat
     9421 accacttcaa tcccacatgt tcattcattt ctttataggc tgtatcaatt cgttccttag
     9481 tcgcttcatc taactggtca cgatgactgc catcctctat aaaatcttcc aaaatataag
     9541 tttggtaatg atacaaggga tatccttctg gtgaaaaggt tccctttggt gttcgattga
     9601 cccaagtagg atgagcttta gctgttccga tagttgtttt tccatccttc ttcttgatgg
     9661 tgacatccat gagaacacca cgttcagtcc acttagcatt ctcttcatct cccatagatt
     9721 caattcgttg attggaaatg aagttcccca tttaatagat aatgagtttc ttatctccat
     9781 ctttttcaac cgtttcagat ggttcaacaa cgtgaggatg ccctccaaag ataatatccg
     9841 ctcccaaatc gatcatcttg tgataaagag ctttttgttc ttcagttggt tccaatcgat
     9901 actcaacacc catctgaagc atgataatgg tgatatctgc ttccttctct gcccgttcaa
     9961 tttcaacctt catcttatct tcgtttaaat ctgaaagata acgattatag tcttcctgag
    10021 aaatatactg ctcaattcca ttgaaaccat aggaataggc taacaatgca accttgatac
    10081 cattcacttc cttaatgacc agcggagcct gatcacgtgg ttcgtgcgta taaactccga
    10141 ttggagtgat tccagctttc tcaataatat cggccgttga aataactccc tcaatttgcg
    10201 aatccaaaat atgattatga gctaaatcca gcacatgata acctgcctcc ttaatagcat
    10261 ccataacttc aacaggagca ttaaagagaa gataacccgc taaataatga tccttattaa
    10321 tggttccttc aaaatcacca atagctaaat ctgctgctta gtccccttgc ttgagccaag
    10381 gagtcacata ctcaaaattt tcatgaaagt cataggtacc gtcttctttt ttagctgaaa
    10441 agaaaagtcc atcgtggtag agcaaatccc cgtgggccat aattctggca gttttttcct
    10501 cttcctactc ctgagacttt tgcttagtcc cctcttgaga aatagtatct cgtttttgac
    10561 tagtcaaggg attccttgga agctttcaaa caacaagacc aagcccattg ataaaccaac
    10621 tgctagcaag agtatcgcca caaatccctt attgctccac ttgcgataac tcctaaaaag
    10681 tttaccaagc ccttcataaa acgaaaagct aaaccaccct gatttcgatt ttgtcttctt
    10741 tgtatcttcg ttctccctac tttcttatgc aagccttttc tttttattat atcacagata
    10801 agtatttctt tcacaattga attgaacttc ccatctattt tctataaatc ctaaatgcca
    10861 taatgctttc aattcttgtc attttgtgat atcatgtaga ggaaatgaac taatccacag
    10921 tggcttattc caagtatacc acttgggctt tggcagtagc taactgcact aaacataata
    10981 taaggagaga aaatgtctgc aatagaacgt attacaaaag ctgctcactt aattgatatg
    11041 aacgatatta tccgtgaagg gaatcctact ctacgcacgg ttgctgagga agtcactttc
    11101 cccctatctg accaggaaat catcctaggc gaaaagatga tgcaattcct taaacattcc
    11161 caagatcctg tcatggctga aaaaatggga ctccgcggtg gtgttggact ggctgctccc
    11221 cagttagata tctcaaaacg cattatcgct gttttggtac ctaatattgt tgaagaaggc
    11281 gaaactccac aggaagccta cgatttggaa gccattatgt acaatccaaa aatcgtctct
    11341 cactctgttc aagatgctgc tcttggcgaa ggagaaggtt gcctgtctgt tgaccgtaac
    11401 gtgcctggct atgttgttcg ccatgcccgc gttactgttg actactttga caaagatgga
    11461 gaaaaacacc gtatcaaact caaaggctac aactccattg ttgttcagca tgaaattgac
    11521 cacattaacg gtatcatgtt ttacgatcgc atcaatgaaa aagacccatt tgcagttaaa
    11581 gatggtttac tgattcttga ataaagaaaa tcccgttgca agacggggtt ttgtgttata
    11641 atagaggcat gaaaacaaat gatattgtct atggtgtcca cgccgttacc gaagctctcc
    11701 ttgcaaatac aggaaacaaa ctctacctcc aagaagatct ccgaggtaag aatgttgaga
    11761 aagtcaagga actagctaca gaaaagaagg tgtccatttc ttggacatca aaaaaatctc
    11821 tctctgagat gactgaaggt gctgttcatc aaggttttgt tctacgagtg tctgaatttg
    11881 cctatagcga gctagattac atccttgcaa aaacacgcca agaagaaaat ccacttctat
    11941 tgattctaga tggtctaacc gatccccata atctgggttc tatcttgcga acagccgatg
    12001 cgaccaatgt ttcaggtgtc atcattccca agcaccgtgc tgtcggagta actcctgtcg
    12061 ttgccaaaac agccacaggt gctattgaac acgttccaat tgcccgagtg acaaacctca
    12121 gtcaaacctt agataaactc aaggatgaag gcttctggac ctttggaacg gatatgaacg
    12181 gtactccttg ccacaagtgg aatacaaaag ggaaaatcgc cctcatcatt ggaaatgaag
    12241 gaaaaggtat ctctagcaac atcaaaaaac aagtcgatga gatgattacc attccgatga
    12301 atggacatgt tcaaagcctt aatgccagtg ttgctgcggc cattctcatg tacgaagttt
    12361 tccgaaatag actataaaaa agtttccagt catctgattg gaaacttttt tatgattaac
    12421 tatgttctgt gataaacttg taggcttctt gaccagcgat agctccatct ccaactgctg
    12481 ttgttacttg gcgaaggtct ttcaagcgaa catctccaac tgcaaagata ccgtcgactg
    12541 cagtcttcat gtggctatct gtcacaatcc agcctgcctg atcttggata ttcaattctt
    12601 taacaaaatc gctaagagga tccaaaccaa catagataaa gacaccaccg aaggcttgtt
    12661 ctgtcacttg acctgttttc acattttcaa atacgactga ttctactcgg ttttcaccct
    12721 tgatttccct tactacagaa tcccagataa agctgatttt ttcattcgca aaggcgcgat
    12781 cttgtaaaac cttttgggca cgaagttggt cacgacggtg aacaatggta acagtcttag
    12841 caaaacgagt caagaagagg gcttcttcaa cagctgaatc tccaccacca actatcaata
    12901 aatcttggtc acggaagaaa gcaccatcac acacagcaca gtaagaaaca ccacgactgt
    12961 tcagttcttc ttctccaggc actcccaaag gacggtgttt agaaccagtt gctacgataa
    13021 ctgtacgtgt ttcatatgtt tggtcatcag tcatcacttt cttaaaatca ccatggtctt
    13081 cgacattttc aacataacca taaatgtgct caacaccaag attttcaagt ggttcaaaca
    13141 tcttttcagc caattcaggt ccactaatat tagcgtatcc tgggtaattt tcgatatcag
    13201 atgtattatt catctgacca cctggcagac caccttcaat caaagctact tttagattgc
    13261 ttcgagcagc atacaaggcc gcagtcatcc ctgcaggtcc agcaccgata ataatagtat
    13321 cgtacatata gattccttct ttcttgttgt aactatcttt attctaactc tttcttgtca
    13381 atcacgcaag gattatgcct tgaaaacaag aattaaactc ataaccgcaa aggataatac
    13441 tgggacaacc aagactccaa tcataatcat atcatagaga tgggcttggc gagccctggc
    13501 tgtcttatca actcccgaca tggctctcag accaatccaa atccctaaaa aaatcagaac
    13561 aaggatggtg gtcaagatca aactctcgaa atataaagaa aatagttgca gtagcatgat
    13621 ttctctcatt tctatctttt ttaaagagta aactcagcta gtccaactaa ctgagttttc
    13681 ctttatctat tatatcaaat ataagtccgt ttgtaactag cgaagaattc ttttgtccgc
    13741 tcttctttag gggtgtggat aatctcatcc ggagttccag actcgatgat tttcccctta
    13801 tctaagaaga gaattttatc cgcaacttgg gctacaaagg gcatgtcatg actgaccaaa
    13861 atcatggtct gacctgactt agcagcatct gcaatagact tttctacttc accgaccaat
    13921 tctgggtcaa gggctgaagt tggttcgtct aagagcaaaa catctggttt catagcaagc
    13981 gcacgcgcta gggcaacccg ttgcttctgt ccacctgata aatggcgagg ataatggttt
    14041 tcacggtccg aaagcccaac cttagccaac tcttccttgg caatcttagt cgcttcttgg
    14101 tcagataatt tcttgacaac aaccaagcct tctttcacat tatcaagtgc tgttcggcgt
    14161 tcaaataaat taaactgttg gaaaaccata gacaacttac ggcgtagggc aaggatttct
    14221 tcttgagtga ttttagaaaa atcaactgaa aaaccatcaa tctgaataga gccactgtca
    14281 ggtgtttcaa gataattgag actgcgaaga aaggttgatt ttccagctcc tgaagaacca
    14341 atcaaggcta caacttcccc tttttgaata tccaagttca gatgatccaa gacagtctgt
    14401 cctgaaaagg atttgcttaa attcgaaatc ttaatcatta acgaaggtct cctttcacat
    14461 ctgtttgcac tgtatcaggt gcagaaatag ccatttttct ctcgatgaaa cgaccgaggc
    14521 tttcaattcc gatattgact acccaataaa caagggcaac ggagatgaag cgttcaaaat
    14581 agcgataatc agctccacct agaatctgag cttgggcaaa gacttccaca acacccgcac
    14641 taaaagctag agatgttccc ttggtcaaac cgatgaggga attaatcaag gttggagtag
    14701 ctaccaccgc tgcattagga ataatcactc gtcgataaac ttgcgctcgg gtcataccca
    14761 gactgcgtgc cgcctcaatc tcaccaggat taactgagag aatggctgca cgaatggttt
    14821 cactagcata agctgcctca ttaaaggcaa aggcgacaat cgcaaaagct gcagctggaa
    14881 tcgcattgat attgagacca gttccccatt gctgattgag ggctttcaaa gccaaaggaa
    14941 ttccgtagta ggtcaacatg agttgcacca aaatcggtgt cccttttaag aaactaacaa
    15001 agaaggcctg caagggatat aaaatcttga cacgattgat cttcacaatg gcaaaaagaa
    15061 gcgccaaaac caagccaaaa agggcaccac caattgtcaa cataattgtt gttggaagtt
    15121 gttggacaat tctagggatt ccatcaaaga ccgaacgtag gctaaacagc ttgccatccg
    15181 gaatcaattg catcaagttt tggtaccaat ctgatgctaa aatcgttgta acattcataa
    15241 aaacatcctc ttctgataat gattcattct accatgcttc tgccgtcaat gaaattattt
    15301 gctacggtca gatacagact ttgtctacct actagtttat catactttga aacagggaag
    15361 ttatatattt tatctatcaa agagatagat aaaaactatt tcagtcccag ttcttcataa
    15421 gattttcgat aaccgatttg cttaacagtt ccattttcta ctaaaatggg ccgttttaac
    15481 aacataccgt cacttgctag caactcagcc gcttcttggt ttgacaaact tcctacctta
    15541 tcttttagcc ctaattcacg gtatttgata ccactggtgt tgaaaaattg cttcaattca
    15601 aatcctgagg tttctagcca attcaaaatg acttcttggc taggtgtttc ttccacgata
    15661 tggacggctt tatagtccac acctaattga tttaattctt gttttgcttt tttacaagtt
    15721 gaacatttgg ggtattcgat aaattctaac atgtgcttct ccttctattg cttctatctt
    15781 taaaatctac tccttcatgc tccaagagcc aagctttctt ttccactcct gcagcataac
    15841 ctgtcagacg cttgcctgct cccaacacac gatgacaagg tactaggata gaccaaggat
    15901 tgcgtcccac tgctccacca attgcttgag cagaagccac ttgcaggtct tgagcaattt
    15961 gtccataggt tactgtctga ccataaggaa tgccctgtaa atagtcccaa actctctttt
    16021 caaaattcgt tccgattggc gccaagagca agttggataa atcctgaggc ttgcctttaa
    16081 agtaatcatc taagcaagca ataactgggt ctaaaatagg atgactaaca acttcttcta
    16141 tcgtttcatc tcctagtccc ctctcaaaat gcttctgctc ctgaacccaa attccataca
    16201 aataatggtc atcagctaca agtgatagaa taccaattgg tgaggagtag agtattttta
    16261 catacttctt ttgcattatt tctttaattt ttgataggcc aagcgttcgc catctactgg
    16321 caccttacca acttgtttaa aaccaagttt ttcaaaaata tgttgcataa ccttgttttc
    16381 agcatgcgta tctgagcgaa aatcaagata atcaaaacct tcaatcaagc cctctaagaa
    16441 ggtttgagca actccttttc cctgcacatc tgctgccaca gcaatacggt gaaagactag
    16501 atactctgac tctccagctt gccagtttcc ctcataaata gcttcatagg cctcctctgg
    16561 actcttggtc acagcagcat aggctagcag ttctccctct tccaaggcta cgtaggcttg
    16621 ccctgagatg atatcatcga taataacatc tgcatttgga tagccatttt gccactggtc
    16681 actaccagca tctgctaaac attttttggc atcctccatc acctgcatga tggcatctac
    16741 ttcatttgga aaagctaaac gaatttccat cttttttcct catttctgac tagtttctcc
    16801 catcatagca taatttaagt atttctacaa gcagttaaaa cttgactttg tgtttttgtt
    16861 attttataat ctagttatca aaactatata aaaaggagtt gaaaatgaaa actacctttt
    16921 cctacccaaa atgggcagaa attccaaaca ttgacctcta tctggaccag gttttgctct
    16981 atgtcaatca ggtctgcgcc cctatctctc ctaataaaga caagggccta acagcatcta
    17041 tggtcaacaa ttatgtgaaa aatggttacc tgacaaagcc tgacaaaaaa aaataccaac
    17101 gccaacagat tgcccgtttg attgctatca caaccctcaa gtctgtattt tctattcaag
    17161 aaatagccca gacacttaat actctacaaa ctcaagcaag ttcagaccaa ctctacgatg
    17221 cttttgtgga ctacatgaac caaggaattg acccagctaa ccctattatc caaaccagct
    17281 gccaaaccat taaactctat catcaaactc tagacttaat cgatcatact caagaggagg
    17341 taatccaatg aacactagtc ttaaactcag caaacaactc agttttggag aggagattgc
    17401 taatagcgtg acccatgctg tgggtgcagt catcatgctt atcttgctgc ctatttcatc
    17461 catctatagt tatgaagcac acggattttt atcctctatc ggcgtttcca ttttcgtcat
    17521 cagtctcttt ctcatgttcc tatcatccac catttatcac tctatggcct atggttcgac
    17581 ccacaaatat gttttgcgaa tcattgacca ttctatgatt tacgttgcca ttgccggctc
    17641 atacacgccc gttgtcttga ccttgatgaa taactggttt ggctatctga ttattgtcat
    17701 ccaatgggga acgaccatct ttggtattct ctataaaatc tttgctaaaa aggtcaatga
    17761 gaaatttagc cttgctcttt acctgattat gggctggttg gttctggcta tcattcctgc
    17821 cattatcagt caaacgacac ccgttttttg gagtctcatg gtaactggcg gactctgtta
    17881 tacagttgga gctggatttt atgccaagaa aaaaccttat ttccacatga tttggcatct
    17941 ctttatccta gctgcgtccg tactccaata catcgctatt gtttattaca tgtaaaaaag
    18001 ttgagaaatt caatctcaac ttttttcttt acacatattg ataaagtact ggtgcaagcg
    18061 cacatcatca gtcaattctg gatgaaaaga acttaccaac atattttttt cttgggctgc
    18121 aacaatttga ttgttcactg ttgctaaaat ttctacaccc tcaccaacac tactgataat
    18181 cggaccacgg ataaaggtca ttggaatctt gccaactccc ttacattctg cttccgtgta
    18241 gaaacttcct aattggcgcc cataagcatt acgctcgacc accatatcca tagttcctag
    18301 atgactctct ttctgagaag tgatttcctt agccagcaaa attaagcccg cacaggtccc
    18361 aaacactggt aagccagata gaatggcttc tcggatggga agtagcatgt tctggtcacg
    18421 taagagcttg cccatggttg tagactcacc accaggcaaa atcaaacccg acaagtcact
    18481 ctgatcttgc tgaaaatcat ctagatttct gagttctaca ctctcgacac ctaattgatc
    18541 tagcactttt gcatgttctg caaaggcccc ttgcaaggcc aatattccga ttttcatcta
    18601 ttttcctcgt tcagccatga gaatttggat ttcattttca ttaataccaa ccatggcttc
    18661 tcctaaatct tcagagattt gagctaggat ttgaggatta cggaagttag tcacagcctt
    18721 aacaatggca ctcgctcgtt taacaggatc tcctgacttg aaaatacctg aaccgacaaa
    18781 gaccccctct gcccctaatt gcatcattaa cgcagcatct gctggcgttg caacacctcc
    18841 agcagcgaaa tttacaactg gcaattttcc atgttcatga acatattgga ccaattctac
    18901 agggacttgc aaatccttgg cagcaacata aagctcgtcc tcacgtaagt tttgaatgcg
    18961 gcgaatttcc tgattcatca tacgcatatg acgaacagct tggacgatat cccctgtccc
    19021 tggttctcct ttggtacgaa tcatggaagc accttcagcg atacgacgca aggcttcacc
    19081 caaatcctta gcaccacaga caaaaggaac ttggaattct ttcttgtcca catggaaacg
    19141 gtcatcagct ggagatagaa cttcactctc gtcgatataa tcaatttcaa tagcctctaa
    19201 aatctgagct tcaacaaaat gcccgattct gaccttagcc attactggaa tactaaccgc
    19261 ttcttggatt tccttaatca tctttgggtc gctcatgcgg gaaactcctc cagctgcacg
    19321 aatatcagcc ggaattcgtt ccaaggccat cacagctgcc gcaccagcag cttctgcgat
    19381 acgagcctgt tcaggattct gcacatccat aataacacca cccttgagca tctgtgccaa
    19441 gtttttattt agttcataac gattttcagt catttgtttt atcctcatca tttgtattgg
    19501 tagctacctt ttcattttaa gtcagaacag aatgaagcac aagataaaaa aactataatt
    19561 gtatggggac agattggcta aaaactatct gaccttaact taaggccaga tagctcattc
    19621 atttctattt ttcagccgta agggcagcca ttgtgatgta gttgtatggt ttgttgaagt
    19681 gtggcaagaa gaagaggtct gtcaatgcca atttatcaat tgtcacatgc tcttggatag
    19741 caagtgagaa catgtggatt cccatgctaa ttgcaatatc atgtgaaacc atttgggcac
    19801 caagaatttc acggctatct ttgtcaaaga caatcttaat tgctacttca tggttgtcat
    19861 gtttcatgaa ttctggtttt tgaagatcgt taaagcctgt ttcagttgcg ttgtaaccag
    19921 cagctttcgc tttttcaaga gtcaaaccag ttgaaaccat gtgaagaccg tagattgaga
    19981 taccatttga accttgaaca ccgattcctt ccaattcatg tccacaagca ttgtaggcac
    20041 caacgatacc agtgcgaaca gcatttgaag caagagcgat atagcttgta tctttacgag
    20101 cattgtcata aacagtcgca cagtcaccaa cagcgtaaac gcctgggata gatgtttctt
    20161 gtttcttgtc tacaaggaag gcaccgttgc ggaagagttc gatcttacca tctgcaaggg
    20221 ctgtgtttgg acggaaacca actgcaagga taaccatatc cacgtcaaag ctttctttgt
    20281 cagtaatcaa gcgttcaact ttaccgtcac cttcgattgc tttaacagtt tgacctagag
    20341 ccaagcggat gttgtgatct tccaagttct tcgccatcat ttgtgtgaag tctttgtcat
    20401 agtaaccgtt caagacagta tcaacgatat caacaaggac aacttctttt ccaagacgtt
    20461 caaaggcttc agcaagttca acaccgatgt aaccaccacc aacaacggcg atacggtcga
    20521 ggtgttggct cttgtcagaa agtttattga taacttcttc agcattttgg tacaatttca
    20581 cgaattgtac gttttcaaga gttgctttaa attcgcggtt tcctttaaca atttcaacac
    20641 cttcgattgg tggcaagatt ggtgtagaac ctgtagcgaa aatcaatttt tcgtatgatt
    20701 ctttgtgctc ttttccttca acttccgctg taattacttt gttatcatag tcgattgaaa
    20761 gaacaggtga gttcatgtaa actttagcac ctttagcttc caatttttct ttatcagaat
    20821 agaacaagcc ttcagcaccg tcaatttgtt caccaatcca aagagccatt ccacatccta
    20881 ggaaagagat gttagagttt tggtcaaata caacaatttc gttctcattt ccaaaattat
    20941 ccaacatggt attgatacat gctgtaccag cgtggttagc accgactaca acgattttac
    21001 tcataaaaat ttcctgcctt cattctaaat ttaccttact agtataccat ttaccgttag
    21061 cgtttacaag gggggtacta ttatttcagc attttttatc aaaaataatt ccccatttat
    21121 cctgttttca agcctgatga attttatgag catatttctt gactttttcc aaattttgtt
    21181 tatgaaaatt ctaactttat ggtattataa taacatgaaa atgaaatgta agggcttcaa
    21241 tttagatgat tatttgatat tacaacttat tttctataag agaaaggagt tggtagcttg
    21301 actcttcgtt cacattctga acggctaatg gggactacta tcactatttc attagtggat
    21361 gaacaagccg atatctttct ccaaaaatcc tttgacttgc tcaaagaact tgaataccgc
    21421 ttcaacgcca atagtcaaga atctgagttg atggaaatca attatcaagc aggaatagcc
    21481 cccgtcacgg ttcatccaga cctgtttgag ctgatttcac ttgggttaga gcatagccta
    21541 gcgctctcta gccacctcaa tatcagcatt ggtcccttaa ttcaaacctg gcgtatcggt
    21601 ttttcagatg ccaaggtcgc ccagcctcaa gaaattgaat cggtgctgcc tctaatcaat
    21661 cctcatggta tcgagttaga ttcttctact tctactgtgt ttttaaaaca gaaaggaatg
    21721 aagatcgatc taggttgttt agccaaagga tacagtgcgg ataaggttgc ccaatttctt
    21781 aggaaagagg gggtgacttc tgccttgatc aatctgggag ggaatatcct gaccattgga
    21841 aaaaatcagg caagagggga taacccatgg caaatcggga ttcaagaccc agccaatcct
    21901 aggggaaatc atttaatgac catccctgtt gtcaataaat ctgtcgtgac ttcaggcatt
    21961 tatgaacgtc acctgaccgt cgatggacaa gattaccatc acatttttga cagtcaaaca
    22021 ggatatcctg ttgaaacgga actagcgagt ctaacaatca tctctgataa atcagtcgat
    22081 ggcgaaatct ggacgactcg actctttgga gaaagacctg cttctatcct ctggcaagtc
    22141 gaaagtttgg agggcatcga agtcatcctc atcgataaag aaggccacct aagctgttct
    22201 tcaggaattc ctactctata gagacaactg tttcaatgga attttaaaat tgtattatgt
    22261 aaaaagagaa aggaaatctc atgttaaaac ttattgctat tgttggaaca aattcaaaac
    22321 gttctacaaa ccgtcaattg cttcaataca tgcaaaaaca ctttgctgac aaagctgaaa
    22381 ttgaacttgt tgaaatcaag accattcctg tcttcaacaa accagctgac aagcaagtac
    22441 ctgctgaaat attggaaatt gctgctaaaa tcgaagaggc agatggcgtt attatcggta
    22501 ctcctgagta tgatcactct attccagctg ttttgatgag cgctcttgct tggttgtctt
    22561 atggtattta cccacttttg aacaaaccaa tcatgattac aggtgcttct tacggtacgc
    22621 ttggttcatc tcgtgcccaa ttgcaacttc gtcaaatctt gaatgctcct gaaatcaagg
    22681 caaatgttct tccagatgaa ttcttgctct cacactctct tcaagcattt aacccaagtg
    22741 gcgacttggt tgaccttgat gttatcaaga aattggatgc catctttgat gacttccgta
    22801 tcttcgtgaa gattactgag aaattgcgca atgcgcaaga attgcttcgc aaagatgctg
    22861 aagactttga ctgggaaaat ttgtaagata ggagaccgaa agaatgaaat ttgttggact
    22921 tgttggatca aactacgatc aatcatataa ccgcaaactc ttggaattta tccgtcgcaa
    22981 tttcaaattc aaatttgaat tagaagtact tgaaatcgac gaagttccaa tgtttaacca
    23041 agatgaaaaa tgggacgaaa gcttccaatt gcgtttcttg tataacaaga ttacacgtgc
    23101 tgatggtgtc attatcgcca ctcctgagca caaccacact atctcagctt cactcaaatc
    23161 tgtacttgaa tggctttcat acgaagttca tccatttgaa aacaagcctg ttatgattgt
    23221 gggtgcatca tactatgacc aaggaacatc acgtgctcaa gttcaccttc gtaaaatcct
    23281 tgatgctcca ggtgttaatg cctacacgct tccaggtaac gaattccttc ttggtaaagc
    23341 taaggaagct tttgataacc acggaaatat caccaacgaa ggcactgtta aattccttga
    23401 aacttgctta gataactttg ttaaatacgt aggagtcgtt tcgaaattga aaaaaccaaa
    23461 accaatcgaa tcagaagact tggattgtgg aaaaccaatc gctacaacca tcacagaagt
    23521 tgaccctgac gatccagaat gggtagaaaa agttgcagca atcactggtg ctgtttctgg
    23581 tgatacctat gtcaaattgg accacggtat cctaacagtt aaccaaattg atatgttctt
    23641 gaaagctatg ccatttgaat tgacatacgc tgacgacaac aaccaattcc tctactacaa
    23701 caacgctcac caagatccag acactatgtt cgctaaacgt gtaccacctc aatcaggtag
    23761 ccgtatgtcg actgttcatg gttctcttcc accagcacgt atgaagaacg tagagtgggt
    23821 tatcggaaca cttcgcaacg gaaaccaaga atacgtccgt acgatcgttc caggttctcc
    23881 tgcaggtgtt atcaacactc acaactacca agcaatgtac tatcctgatg gatcatacgc
    23941 tggtatcaat gaaatcgtct ttaacttcca accatggctt gactggtacc taaaagagac
    24001 tggtcaacgt ttggtgggtg gtagcggacc atttgctcct gctgccggag atcatggaga
    24061 cgccgatgct acttctggcg cttctgattc aggggatgct ggaggccacg gtggtgacgc
    24121 agacgctaca gccggtgcaa gttactaatg atagttttta ggaaccatta tggttccttt
    24181 tttagcgaaa aaagactaac aaacacaaac tgctagtctc tttgttatcg tttcatctat
    24241 ccttgttaaa agaaagaata cttatactca atgaaaatca aagagcaaac taggaagcta
    24301 gccgcaggct gtacttaagt acggcaaggt gaagctgacg tggtttgaat ttgattttcg
    24361 aagagtatta ttatgaattt gggtcatcaa gactacggcc atgtaatcct ttttcacgtt
    24421 gtacttggcg tagtttttct ggtgtaacat cgtttccttc ttcgtccaca attttgattc
    24481 cttcaatgtg gtgacgaaca gcgcggcgat aaccttcgat gtactcctca cgtagtttgg
    24541 cttgttccac tttttcttct ggtgttaagc cttctgtttt tttcttttta gcaagctcat
    24601 tgatacgagc aatttttttc ggatccattt cttctccttt gtaataagat aaagtccgtt
    24661 tatcaaattt tatttagtgt taatttggtt aaaacaagca aacttagctg ctttcttggt
    24721 tagttgtgac aagattgcca aacgattttg acggacagcc tgatcttcag ccattaccat
    24781 agtattttca aagaaagcat caatgactgg gctaagcgca aagagttgtt tcaattgctg
    24841 acttgcaggt cctgataaaa cgagtgtttc tactgcttct gccaaagctt tttcttggtc
    24901 attctcaaat agtgctgaat caactgtagc aaccccttct gccttctcag ccaggttaaa
    24961 ggcacgagaa agtgattcaa cagatggttt aaaatcttct tccttgctta cttctacgag
    25021 agcacttgct gcttccaaca tatctgccac aacaaagttt gaacctgcaa gaactgcttc
    25081 cttgatatct tttggagtag agcccatcat cttatcaaca cgagccttga taaagtccat
    25141 aacctctgct ttattttcat aagtcaaact gtcaaatttc aatgcataaa ggctatcaat
    25201 cagctcatcc atagcaatgt gccaaccaaa ggcatccaag atacgaacca caccttgagt
    25261 tgcacgacga agggcataag ggtcattaga acctgatgga atcaatccta ctgagaagaa
    25321 actcaaaatc gtatccaatt tgtctgcaat ggctagaact gcgccgacct tgctctctgg
    25381 aagttctcct tcagctgatg taggcatgta gtgttcacga atagcagctg ccaccgctgg
    25441 agtttcacca gcaagaaggg tgtatttttc acccataatt ccttggagtt cgtcaaattc
    25501 accaaccata cctgtcaaca agtcaaactt gtaaatggct gctgcacgag caaggtcaac
    25561 tgtttcatcc actgacaaac cagctttttc tgccaaaagt acagtgattt gacccgtacg
    25621 aatcatgtgt tcacgaaggg aaccaatctt ctcatggaag gtgacattgt ttaatttttc
    25681 aacaagatct gaaatcacca atttttggtc ttcacgccag aagaattctc cgtcttccaa
    25741 gcgagctacc aagacttttt catttccttt aatgacattt ttcaaacgct ctgcgtttcc
    25801 gttacgaaca gaaatgaagt ttggcaagag ttttccatct tgatcacgaa caacaaagta
    25861 acgctggtgt tccttcatag aagtcaccaa gacttcttct ggaacttcaa ggtatttagc
    25921 atcaaaactt cccatgaagg cagttgggta ttcaaccaaa ttcaagactt cattcagcaa
    25981 atccgcatca atttcgatac gtacaccatg ttttgcctca atttccttga tttggtcaac
    26041 aatcatttgc tcacgttcac atggatctgc gattacaaac tgcttacgaa ggtcttcttc
    26101 atagctcaat gctgactgaa ttttggtttc tttccccaag aaacgatgac cacgactcac
    26161 acgacctccc ttgatatcaa ggaaatccaa atcaaactct tcttcatcta aaagaacagt
    26221 taaagtgtga acaggacgaa tgtattcaaa gctatttcca gcccagtgca tgctgacagg
    26281 aaaagtcagt gacttcaaga catctacaac accaggaaca atggcttcaa ctgattggcc
    26341 aacttcttcc ttagtgacat agacatattc ttcaccctta atttcacgga attcgatatc
    26401 ttcaacagtc aaacctttcc cacggacaaa tccttgagct gctttggtga agtttccatc
    26461 actatccaag gcaattttct ttgctggtcc cttgaaatct tctgtcaaat cagactgttt
    26521 gtctgataaa ccagtcacac gaacagccaa acgacgtggt gttgagaagg tttgaatggc
    26581 ttcaaaagac aggcggtttt ccttgaggaa ggctgccatt ttttcgccta gttgtttttc
    26641 acttggtgtg acaacatagg ctggtaattc ttcaagaccg agttctacta ataagttttt
    26701 tgtcatgttt tttcctttac attttcttca atcttttttg atatgcacct taggtactgg
    26761 ggacgtcgct aactaactct gtgagtctgt atggaaatcc aatgctaaat ttatcaagat
    26821 ttccaacaat ctcataaaag tggatttagc tgactgattt ttgaacctaa ggtttcaaaa
    26881 atccccacat aacagtacgg cggtgcatat ccctctagca agtcttcgac ttgcctctaa
    26941 cgattttaga gtacagtatt actcactatt ctgcgtcttc tgctaaaagt ttaactcgtg
    27001 tttcttcatc caaaagtggg taacctaggc gtttgcgttc tgcaacaaag gttttggcta
    27061 caacacgggc caagttacgg atacgagcga tatagcctgc acgctctgtt acagatacgg
    27121 caccacgcgc gtcaagcaga ttaaaggtat gtgaacattt gagaacatag tcataggcag
    27181 ggtgtaccaa gccttcttct aatgcacgac cagcttcttt ttcaaactta tcaaagtttt
    27241 caagcaacat ttcttggtcc gaaatttcaa atgaatattt tgagtgctca tactcaggct
    27301 ggataaagat ttctccgtat tttacaccat cagcccactc gatatcatag acagagtcta
    27361 cttcttgaat gtaagaagcc aagcgctcca aaccataggt aacttccgca gtcacagggc
    27421 cagttgccaa tccaccgact tgttggaaat aagtgaactg agtgatttcc attccgtcaa
    27481 gccaaacttc ccaaccaaga ccagctgaac cagttgatgg gttttcccag ttgtcctcaa
    27541 caaaacgaat atcgtgctcc aaaggattga ttcccaattt ttccaaagac tcaaggtaaa
    27601 gttcttggat atttgatgga gaaggcttca tgaccacctg gaattggtgg tgttggtaga
    27661 gacggttagg gttttcccca taacgaccgt cagcaggacg acgtgatggc tctacataag
    27721 ctgcattcca tggctcaggt ccgatagcac gaaggaaagt gtaaggactc attgttcccg
    27781 cacctttttc attgtcataa gcctgcataa gcatacaacc ttggtcattc caaaatcgtt
    27841 gcaaagtcaa aataatttct tgaaatgtta atttcttaga cattgaatac tccttaataa
    27901 aaaataattt cttgcgacac gagtctacct cgtcgattat acgaggcaag acgagttagt
    27961 actgcgtcta gctcagcacc ctagtgctga gcgtggggca gtccgactgt aaggaggtct
    28021 ctgccactga tgcagtgaaa tgtcaatttc ttagacattg tttactcctt tagtttttat
    28081 atttcttttt caattaattt ctgctgaaca aaccaatcta acaagggaaa agttttttct
    28141 gtatttgccc ataaatgata agaatctaag accgcttgta cactacctaa attttctacc
    28201 aacttatcta ctgtcaaaaa gctgcgccag tattcataaa aaatacttgc atagttctta
    28261 tcataagtcg aagaaccaaa atcattcaat gaatgccaac catacttctt ctgaaaaagt
    28321 tctactagag attgattaca aattttttcc gcttgaaatt cctcttctgt caagaaatac
    28381 ttgcgactaa tatattcaac catcccctct tcgaaccaga tataagagtc ataaccatca
    28441 aaatcatcta aaaaatgctc cgaccaatga gctaactcat gtcctacaat ctgtaagagg
    28501 aaattttcag ataaagaatg atagtgactt tctattgctt gagtttgctg agaacactcg
    28561 tagtccatca attgatacaa atacaactct ttccaaacag ctaaatcagg cgtcataacc
    28621 attcgtctat tatttgtata ggctggaaca gcgctttccc taataatctg tgtagcagca
    28681 ttaaaatttg accaaataac ggcttgcggt aaatcacaga ctgcaagttc attctttaaa
    28741 aaagattgat aatctttcaa cttttcagta tttcttacta caaaatcacg aaatgcagct
    28801 agttgactat cgtcttttac aagataaagg ttttccacgt ctctcctttc tttcacacaa
    28861 tagactgaat aagcaaaaga accacatctg acaatcctag gttgatttaa cctaggggcg
    28921 tcagaaacgc ggttccaccc taatttatta cttcattgtt tttgaaaata ctacagaaag
    28981 cgccagctca ttattttgcc ctacttggct cccactgtcc caagctcgct tgaagaacgc
    29041 cataagactt tctttcctgc tgactattat atcaaaaatt agaataatgt acaattcttt
    29101 ttatgatttt ctagaaatcc acatcatcaa ctcgtggagc acccgattga acagcaatcg
    29161 tttttaaggt ttctctctcc tcatgactca gttcaattcc aaaacaatca agattagctt
    29221 gaatacgaga ggttgtgaca gattttggaa gtggtaaaaa tccttctgcc aagctccagg
    29281 ccaaggctat ctgagcaacc gattttccgt gatttgctgc tatttcttgg acttgcttgc
    29341 tatcaaacag ttctccttgt ccaaaaggcc cccaagcttc caataaaatt cccttttcac
    29401 gacagtaagc tacgacttga tcttgataca cacctggcgc caagcgaact tgattgaccg
    29461 caggaacgat agttgcagtt tcaagcaagg catccaaatg atggggaaga aaattgctaa
    29521 cgccgatagc acggattttc ccttcttgat agaggtcttc catcgctctc caaacttccg
    29581 cattgcgagt tttccatgcg tcattttctc tgagcggttt tgggttcggc caatgaatca
    29641 aatacaaatc caaataatcc aagcccagtt tttctataga tttttccaaa gcttgacgag
    29701 tttgctcata ggtttgctga ctattccaaa gcttggtagt tacgaacatt tcttcacgtg
    29761 gaactccgct atctttgatt gcttgaccaa cactttcttc attctgataa atcgccgccg
    29821 tatcaatatg acgataacca gccttcaagg cttctaacac tgcacgatag gcttcttctc
    29881 catccttagc cttaaaagtt ccaaatccca atactggaat ttctactcca ttatttaatt
    29941 gataggtatt catatgcttc tccttctatt ttattcatca attatagcat aaaacatttt
    30001 ttaagtagta ctatcttctg taaaaaataa gtctgtcaaa tatctaacag acctattttt
    30061 tcttgcttat tcatcacgac tatagtacag ctcatgagct tttaggcgag tattgagcat
    30121 ctctggtatg gtcacaaagg tataaccttg atttttcaaa tactcaatga cccttggcaa
    30181 ggcattgact gtcggactgt gaatatcatg catcaaaacg atagagccgt tagctacttg
    30241 atgctgaatt tctgtcaaaa tagatgcttc atttttactc ttccagtcca gactatccac
    30301 atcccacatg ataaagctca aatccaagct attgcgaata tcatctgtaa tagcaccata
    30361 aggtggacgc atgagtttag aactagaacc cagcacttta gttagcacat cctcagtatc
    30421 agtaatctgc tttttagctt catcaagaga gagttgcgag agaatcggat ggctccaact
    30481 atggtttcca acaacatgac cttcagattt tatcctcttc accaagtcct cattcccaga
    30541 aacatttttc ccaagcacaa agaaagtcgc tttaatatca tatttagcta gggtctctaa
    30601 tacctgcggg gtcgttgctg gatttggacc atcatcaaag gttagagcga caactttttg
    30661 atgtttctta tcaaagtaag attggtacaa ggccgcatct ttttcgagta agtacgaaga
    30721 ttggataaca tcaaagaaag cagatactgg caaggctatc tcttctaaat tttcaaccac
    30781 aggacttgga taaaggataa tctgactatc cttgtaatca aaattccatg cagacaagtc
    30841 ttggtcagag aagtttttta caatctgctc actctggtct tgctctattt ttttatcctc
    30901 tatgaaggag gtcaactctt ttatcagctg ttccttagcc ttactagcat ctgaaaacag
    30961 ttggtcaagt gtaaaaggtt gcccatcttc tgttaagtga acctttccta gactggtctt
    31021 ttcagtctct tcaatcttaa aagcaactaa atcatagatt tgtttggtca cattacgatt
    31081 aacgactccc tttaaacctg actcttcttg ctctgtatag tagaaaacaa gattgtcctt
    31141 actttctagc ttgtccttaa catcttgatt tatcagctcc ctaacagagg aaatcacttt
    31201 ctccccttgg agaggataat aggcaatcac ttcggcttgc ccctgacgaa aatgctcctt
    31261 ctgatttccc tcactcaatt gatcatcttt ctcttttttg agcgattcaa tcttttgctc
    31321 aaaacttttt tgctggtaga tcttaaaagc aataaatccg cctaataggc aaatagaaat
    31381 tgctaaaata ccaatcaaag ccaataatac atgtctcgtt ttcccgtgtc tgccacgtcc
    31441 tagtctactt ttattcatat ttatatcata acaaatttga gcaataattt caatgataca
    31501 atttggcaat ccacaattcc tctagcaagc caaagcatct tacatcaact ccaacatccg
    31561 tatctgtaga gattatgtag ttttctatct atggaatttc tagtagattg aagctagaat
    31621 agtagaccta tacttctaaa acattgttag aaatcgattt gactgtcctg atcgatttgt
    31681 cctgttctca tttcatttca ctataaaact atgaaaagct aggtcatctg agaagtaatt
    31741 actatcgaca attgcgatag cgtatactag tgcgatttgt ttgtagctct ggtgggtatc
    31801 accttcacgt tggcgaattt tttctaggtt ttgattaacc tgaatgcaca gataagccca
    31861 taggcgattg atgaaaaaat tctgatgatg aacctgaatt tcgatgataa cttggattcc
    31921 attatctaac tccgccaaga cgtctatact agtatagaaa tcttgcgctg agtagggcat
    31981 ggaaggcaag acgtgaatgt tacttccctc caaaatcgtc acattttttg ctggcaagtc
    32041 cagcatatcg cggataaatt gacaaatcat tagttgggct gatacctgga tgtctgagaa
    32101 tcctcctttt cctccttcta ttatagcaca tttttaaatc taggtttgct agattctatg
    32161 cttctatcta tttattcgga aaagaaagct aatagcttat ttgaaatttt ctcctctctg
    32221 ttggatagtc ccgtctatct atgttataat gaaagaagga tttaaaatcg gaaaaggagt
    32281 atttatgcta aaattaggtg tcatcggaac tggcgctatc agccatcatt tcatagaagc
    32341 agcccatacc agtggagaat accaactggt cgcaatctat tctagaaaac tagaaactgc
    32401 agcaaccttt gcttctcgct atcagaatat ccaactattc gatcaattag aagtcttctt
    32461 caaatcttct tttgatttag tctatatcgc cagtccaaac tccttacatt ttgctcaggc
    32521 aaaagctgcc ttgtctgctg gtaaacatgt tattctcgaa aagccagctg tcactcaacc
    32581 acaagaatgg tttgatttga ttcaaacagc tgaaaaaaat aactgtttta tctttgaagc
    32641 agctcgtaat taccacgaga aagcctttac tactatcaaa aactttttag cagataagca
    32701 agtgttagga gcagatttca actatgccaa atactcttcc aagatgcctg acttgttggc
    32761 tggacaaaca ccaaatgtct tttcagaccg ttttgctggt ggagccctca tggatttggg
    32821 gatttatccc ctctatgctg ctgttcgtct ttttgaaaaa gctaatgacg cgacttacca
    32881 tgctcaacag cttgacaata gcattgacct aaatggagat ggtatcctct tctacccaga
    32941 ctatcaagtt cacatcaagg ctggaaaaaa catcacttcc aatcttcctt gcgagattta
    33001 tacaacagat ggaaccttga ctctcaacac gattgagcat attcgctcag ctatttttac
    33061 cgaccaccaa ggaaatcaag tccagcttcc tatccaacag gctcctcata cgatgactga
    33121 ggaagtcgct gcatttgcac acatgattca gcaaccagat ctgaatctct accaaacttg
    33181 gctgtatgat gcaggctctg ttcatgagct actatatacc atgcgccaga ctgctggcat
    33241 tagatttgag gcagaaaaat gaaaaccaaa ctaccgactg aatggcaaga actgagtgac
    33301 caactcggtt tccaagaatt cacccccatt caaactcaac tatttgagcc ccttcttgct
    33361 ggagaaaacc tcctaggagt gagccaaacc ggaactggta agaccctagc gtatctccta
    33421 ccaagtcttc tcagactaca aaagaaaaaa gcccaacaac tcttgattct agcaccaaac
    33481 acggaacttg ctggacagat ttttgatgta tgtaaaacgt gggcagaagc tatcggattg
    33541 actgctcaac tcttcctttc aggttcaagt cagaaacgac aaattgaacg tcttaaaaag
    33601 gggccagaaa ttctgattgg aactcctggc cgtatctttg aactcattaa acttaaaaaa
    33661 atcaagatga tgaatgtgga aaccatcatc ctggatgaat ttgaccaatt actagatgat
    33721 tctcagattc actttgtaga aaaaatcact cactacgcac ctcgtgacca ccaactggtc
    33781 tacatgagtg ccacgaccaa gtttgaccaa gaaaagattg tgccgaatac acgcactatt
    33841 aatctctctg accaaaaatt ggacaatatc cagcatttct acatgcaggt agaccaacgt
    33901 catcgagtgg atatgctacg aaaactggct catgtagagg atttccgtgg tctagtcttc
    33961 ttcaatagcc tgtcagacct tgggaatgcc gaggaaaaac tacagtatcg tgatatattg
    34021 actgtttccc tcgctagtga tgttaatgtt aaatttagaa aaatcatctt agaaaagttt
    34081 aaagataacc aactaaccct gcttcttgca actgaccttt tggctcgtgg aattgatatc
    34141 gatagcctag aatgtgtcgt aaactttgat attcctagag atagcgaaac ctacacacac
    34201 cgtgctggcc gtactggccg tatgggcaag gaaggatatg ttattactct cgtcactcat
    34261 ccagaagaaa tcaaaaaact taaaaagttt gcaagtatcc gagaaattgt cctaaaaaac
    34321 caagaactct atatcaaata aagccccatt gctctataca catccagtgt atggagcttt
    34381 tttataagca attcaatttt atactcatct gctttcaaaa agcattctat tccatctccg
    34441 attaacgatg gactttatca cctccttctc cagtccttgt atgacatctt ggagttgatt
    34501 catgacatct tccgaagttc gaaaggcttt attcttaaat ccacgtttac gaatctcttt
    34561 ccacacttgt tcaatgggtt catctctggt gtgtatggag gaataaaggt aaaa
//
LOCUS       DBERMR010000017        34435 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.017, whole genome shotgun sequence.
ACCESSION   DBERMR010000017 DBERMR010000000
VERSION     DBERMR010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 34435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34435
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.017"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(777..1646)
                     /gene="gla"
                     /locus_tag="ACL8A1_000914"
     CDS             complement(777..1646)
                     /gene="gla"
                     /locus_tag="ACL8A1_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaglyceroporin Gla"
                     /protein_id="HGS1201706.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTENPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIVA
                     AIAAVAVFKFLYL"
     gene            complement(1870..2391)
                     /locus_tag="ACL8A1_000915"
     CDS             complement(1870..2391)
                     /locus_tag="ACL8A1_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HGS1201707.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVVSGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPDPGTPKTETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAS
                     LGLGFFKKKEDEK"
     gene            complement(2349..3455)
                     /locus_tag="ACL8A1_000916"
     CDS             complement(2349..3455)
                     /locus_tag="ACL8A1_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HGS1201708.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGSYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(3566..3820)
                     /locus_tag="ACL8A1_000917"
     CDS             complement(3566..3820)
                     /locus_tag="ACL8A1_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201709.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRTTNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRAYKLLKTLIPGENYLSC"
     gene            complement(3814..4056)
                     /locus_tag="ACL8A1_000918"
     CDS             complement(3814..4056)
                     /locus_tag="ACL8A1_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201710.1"
                     /translation="MVQNEDLYKKGLNVELAHQQIKGFFEAEFKNRINGVLNTKIKNS
                     TLNRVNKKTIHQSNKNSMINLKQKQRKMLKNKAILC"
     gene            complement(4160..5005)
                     /locus_tag="ACL8A1_000919"
                     /pseudo
     CDS             complement(4160..5005)
                     /locus_tag="ACL8A1_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078372828.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            5239..6957
                     /locus_tag="ACL8A1_000920"
     CDS             5239..6957
                     /locus_tag="ACL8A1_000920"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HGS1201711.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDVEYLKEVKDVNI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKITNIEAE
                     INAFVK"
     gene            7068..7415
                     /locus_tag="ACL8A1_000921"
     CDS             7068..7415
                     /locus_tag="ACL8A1_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HGS1201712.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFIHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(7694..8530)
                     /locus_tag="ACL8A1_000922"
     CDS             complement(7694..8530)
                     /locus_tag="ACL8A1_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS1201713.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGSRKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(8543..9172)
                     /locus_tag="ACL8A1_000923"
     CDS             complement(8543..9172)
                     /locus_tag="ACL8A1_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGS1201714.1"
                     /translation="MLELRNINKVFGDKQILSDFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(9182..9823)
                     /locus_tag="ACL8A1_000924"
     CDS             complement(9182..9823)
                     /locus_tag="ACL8A1_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGS1201715.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(9969..11198)
                     /locus_tag="ACL8A1_000925"
     CDS             complement(9969..11198)
                     /locus_tag="ACL8A1_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGS1201716.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLVL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(11188..12354)
                     /locus_tag="ACL8A1_000926"
     CDS             complement(11188..12354)
                     /locus_tag="ACL8A1_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGS1201717.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            12498..13511
                     /locus_tag="ACL8A1_000927"
     CDS             12498..13511
                     /locus_tag="ACL8A1_000927"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HGS1201718.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FVRMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(13556..13975)
                     /locus_tag="ACL8A1_000928"
     CDS             complement(13556..13975)
                     /locus_tag="ACL8A1_000928"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HGS1201719.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(13986..15392)
                     /gene="atpD"
                     /locus_tag="ACL8A1_000929"
     CDS             complement(13986..15392)
                     /gene="atpD"
                     /locus_tag="ACL8A1_000929"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HGS1201720.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(15478..16356)
                     /locus_tag="ACL8A1_000930"
     CDS             complement(15478..16356)
                     /locus_tag="ACL8A1_000930"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HGS1201721.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(16372..17877)
                     /gene="atpA"
                     /locus_tag="ACL8A1_000931"
     CDS             complement(16372..17877)
                     /gene="atpA"
                     /locus_tag="ACL8A1_000931"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HGS1201722.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(17892..18428)
                     /locus_tag="ACL8A1_000932"
     CDS             complement(17892..18428)
                     /locus_tag="ACL8A1_000932"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HGS1201723.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(18428..18922)
                     /gene="atpF"
                     /locus_tag="ACL8A1_000933"
     CDS             complement(18428..18922)
                     /gene="atpF"
                     /locus_tag="ACL8A1_000933"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HGS1201724.1"
                     /translation="MHVTVGELIGNFVLITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(18936..19652)
                     /gene="atpB"
                     /locus_tag="ACL8A1_000934"
     CDS             complement(18936..19652)
                     /gene="atpB"
                     /locus_tag="ACL8A1_000934"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HGS1201725.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKIQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(19687..19887)
                     /locus_tag="ACL8A1_000935"
     CDS             complement(19687..19887)
                     /locus_tag="ACL8A1_000935"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HGS1201726.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            complement(19962..20969)
                     /locus_tag="ACL8A1_000936"
                     /pseudo
     CDS             complement(19962..20969)
                     /locus_tag="ACL8A1_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(21414..21812)
                     /locus_tag="ACL8A1_000937"
     CDS             complement(21414..21812)
                     /locus_tag="ACL8A1_000937"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1201727.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
     gene            complement(22140..22622)
                     /gene="greA"
                     /locus_tag="ACL8A1_000938"
     CDS             complement(22140..22622)
                     /gene="greA"
                     /locus_tag="ACL8A1_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HGS1201728.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            complement(22693..24348)
                     /gene="mltG"
                     /locus_tag="ACL8A1_000939"
     CDS             complement(22693..24348)
                     /gene="mltG"
                     /locus_tag="ACL8A1_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HGS1201729.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     IRDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQTVGQLQGDFKESLTAEAF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            complement(24429..24923)
                     /locus_tag="ACL8A1_000940"
     CDS             complement(24429..24923)
                     /locus_tag="ACL8A1_000940"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1201730.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            complement(24920..25384)
                     /locus_tag="ACL8A1_000941"
     CDS             complement(24920..25384)
                     /locus_tag="ACL8A1_000941"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1201731.1"
                     /translation="MEIPIKIIQASKFDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQCLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEEILLYATSQGYSMIWFNPFYLEEQ"
     gene            complement(25394..26728)
                     /gene="murC"
                     /locus_tag="ACL8A1_000942"
     CDS             complement(25394..26728)
                     /gene="murC"
                     /locus_tag="ACL8A1_000942"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HGS1201732.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            complement(26739..27356)
                     /locus_tag="ACL8A1_000943"
     CDS             complement(26739..27356)
                     /locus_tag="ACL8A1_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HGS1201733.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPITEEKPVKPIEKQVEKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            complement(27404..30502)
                     /locus_tag="ACL8A1_000944"
     CDS             complement(27404..30502)
                     /locus_tag="ACL8A1_000944"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGS1201734.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVSDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRICLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPRQATQVQADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            complement(30595..31761)
                     /locus_tag="ACL8A1_000945"
     CDS             complement(30595..31761)
                     /locus_tag="ACL8A1_000945"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HGS1201735.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDGLFEIDFDQLEKDLVEEE
                     VKLYILCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
     gene            complement(31769..32863)
                     /locus_tag="ACL8A1_000946"
     CDS             complement(31769..32863)
                     /locus_tag="ACL8A1_000946"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HGS1201736.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLATIESADYALATSSGMSAIVLAFSGFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGRFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQAIEG"
     gene            complement(33020..33613)
                     /locus_tag="ACL8A1_000947"
     CDS             complement(33020..33613)
                     /locus_tag="ACL8A1_000947"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS1201737.1"
                     /translation="MRLLERFYKPLSGDILMEQSSIYDFNLKEWRSKIAWVSQNNAVL
                     SGSIRDNLCLGLNRLVTDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRFLSGGQSQ
                     RLQIARTYLKDAEILIFDEATANLDADSEYAIISSLYSVLKEKTVVIIAHSLSTVKDV
                     DCIFFLEEGKITGSGTHKELLENHERYARFVQEQMIE"
     gene            complement(33601..34430)
                     /locus_tag="ACL8A1_000948"
                     /pseudo
     CDS             complement(33601..34430)
                     /locus_tag="ACL8A1_000948"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT        10087 a         7580 c         6260 g        10508 t
ORIGIN      
        1 aaaaatttaa ctaattaata gaaagtacta aatagactta tccttcaagg agttttcagc
       61 attatccatc cactcacttg ctgaaaatgt aaacagtctc tggtttatat agtaaaatga
      121 aacaagaata gagcaaatcg atcaggacag tcaaatcgat ttctaacaat gttttagaag
      181 tagatgtgta ctattctagt ttcaatctac tatacaacct caaaactgtg ttttgaacaa
      241 cctgcggcta gcttcttagt ttgccctttg attttcattg agtataaaat tgcagataaa
      301 acgataacaa tcataggact cagcagagtc aatgtaggtc gaagaatacc cccctaatcg
      361 cttattttat agcaaaatga aatagaaact gaacattttg ttgaggaagg taaatcaatt
      421 cctcacaatg tttgagaaag aagggaggac tattccaact tcaacccact ataaagcaga
      481 cttacaagat agggaaccca tctccgataa tatatagtag aatgaaaact gcacaatttg
      541 attggagaat tcaaaccaat ttctcacaat gttttagaaa caagggtcta ttatttcaac
      601 tgcaacctac tatagtccat tgaagctgga atagcccact gagacttcta aaccactgct
      661 ggagaggagt ttgtttagag tttctttcat ttccctaaaa aatcaaaaag agagaaccag
      721 tctgatcctc tcttacttat agatggtgct cactcaaatg ttaaaggagc tatttcttag
      781 agataaagga atttgaatac agctactgcc gcaattgctg ctacgatagg tgctactact
      841 ggtacccaag aataccacca ttttgaatcg cctttatgct caccaagaac tgattttgga
      901 aggaaagcat gaaggagacg tggtcccaag tcacgggctg ggttcaaggc aggtcctgta
      961 ggtcctccaa gtgatgttac caaagccata acgaggaatc caagtgccaa gtgagccaca
     1021 gaaagtcctg aagcagtgtg tggagccacc tgtgctttaa tagccaagtc agaaaaatca
     1081 actgtttgtc ctgcttctat tgccttttgt ttcatgaatt gaagcacttc agcaccaaag
     1141 aagtttttag tcaaaccaag agctgcaaag aacaaaacaa atgaaccaac aaactcatta
     1201 atcaaaccat tgacagttgc tgcatagcga ctttcttttg taccatggtc aatacttgaa
     1261 atagttgaga aagttcccaa gatgttattt gggttttcag ttttcaagta gaatggacgg
     1321 tatgttgcca caactaaggc ttggccaaag atagctccca agacttgcgc gataatataa
     1381 ggtaccactt gtgcccaagg gaaaagaccg ctaactgcaa gccctagagt gaaagcaggg
     1441 ttgatgtgat tcccagatac gttaccaaac atcaaggctg ggatcataac ccccatacca
     1501 taaccaacag cgatgacgat ccagccactt tggtgacctt tcgtaccttt aagttcaacg
     1561 ttggcaactg caccattccc aagaatgatc aaaatggcag ttcccaaaaa ttcagtggca
     1621 tacttcagtg cccatgtgaa atccatttgc tagattctcc ttaaaatttt ttagatattc
     1681 cttattttat caatttttac cctatttagc aactgatttc tggaaatatt tttaaaaaaa
     1741 taggattcat agtttcccag ttcaacggac ttgtaagcgt ttttattctt cacgaaagac
     1801 ctcgttctca aaaccaggaa aaaagcccac atctgtgagc ttttcctggt tcctagattc
     1861 taaaatctat tatttttcat cttctttttt cttgaagaat cctaatccta gactagctaa
     1921 caaggtcatg ataccagcac tagccaaggt agcattagct tctgtacctg tgtttggcaa
     1981 ttcctctctc ttacctgtct cataagtcgg aacttctggg tctggattca ctggagtttc
     2041 agtttttgga gtacctgggt ctggagttgg tttatctggt gttgataaac ggtcatacct
     2101 taccgttatt tctttatcac tagagtctga cgtaacttct tgtgattcaa ctgttggaat
     2161 atctggatct ttgtacttgt caatcttacc agatacaacc tcgtcccagt ttcctgttgt
     2221 ccattcaccg taggttacaa ctcccgtgac cttgttctca gtttttgtac ggcttaaggt
     2281 tacaggttga acaacatctt cttttacatt ttggttcgta actttatcaa cgtaatgaat
     2341 gatacgcgtt atagtcttcg tctcagtaga ggttgctgtt ttgggaacca ctgtttcctc
     2401 aacattctca cggtagtaat agtcaactgt tgcaccgtct tctggtacgc atttgcagga
     2461 gttgctacca aggtgtatgt tgttttcctt gtgataactc ggtcttcttt gtcctcagtt
     2521 gttgttttcc cttcaatagt ttttgattct gtggtatact cagaacctat cgctaaatca
     2581 gcttttataa cagactctgc caacttctct tggctacctt ctttatagta attggctgtt
     2641 actgtagcag tggttggcgc ttcgctttct ctataaacta aggtcactgt tctaccttcg
     2701 cttacaatat tcccagttaa acttgcagaa tttgtatctg cttctttaaa agtataatat
     2761 tttccgtcag tagtagtcat gctactgagt tttttatctg tgacattgta gctggtacca
     2821 atcagttgtt ttttattggt aatgtagctt ccgtcacttt ctttttctcc aattccagta
     2881 tcatttttca atgatagcaa acgcccttgt tcatcaacat agcgaacttt cacattttct
     2941 gagattagtt ctgccaattc tgaggttttt ttctttttct tgatttcttc ggttattttc
     3001 cctttctctt cttcgggaat atttagtttt ggaatgattt tttcaacaac ggttcgtgat
     3061 ggttccacag tatcttggat gactgaaaag tcagctagaa ttgggagatt atactgaaca
     3121 cggtgacttt gagtgtttac tcctactctt tcattattct ctgaaaatac ttgtacggta
     3181 taagaaacaa catcttttcc taatagaaca tccccagtag agaaatagcc gccttttcct
     3241 agtttgctat ctccagagcc cacttctttc ctaatcttat cagatagttt tttaccagtc
     3301 agtacattcg ttcgcacaat ccctttgtct acccctacaa agtgggagaa ctttttgaac
     3361 tcttcagaac cagatctagc ccaaccatta ttaagggcat ttgcttttgt atttgtattc
     3421 tctctcaaaa gtttggcgat tagaattata ttcatcggca cttagagttg ctgctatatc
     3481 tgactcttga ataccaactt ccttactacc atttctagcg gcagtatatg tgtatttatt
     3541 tgtttctact tctagctgat tatccttaac atgataggta attttctcct ggtataagag
     3601 tcttcagtag tttatatgct ctgtcatcat ctttagcttt agaagcagct gagtttagcc
     3661 aagttggggt attaggagta tctttatcag cctgtttcaa gacattattc aagtcatttg
     3721 ctagcctatt ggttgtcctc actgtgaaag ttttcttcca gtctgaacca cttttattag
     3781 acttagatat gctcatgaaa tcctggtcaa cattcaacac aatatcgcct tgttttttag
     3841 catcttccgt tgcttctgct tcaaattgat catggagttt ttgttgctct ggtgtatagt
     3901 ttttttattt acacgattta atgtactatt ttttatttta gtattaagaa ctccattaat
     3961 acgattttta aactctgctt caaaaaatcc cttaatttgt tggtgcgcaa gttcaacatt
     4021 caaacctttc ttatacaagt cttcattctg aacgatttcg gctacacggt gaatggtttg
     4081 atcgtatacc gtcttctctt aaccagcagt atccttagtt ttaatgacta ctcgataagt
     4141 tcctttagaa ccgttatagt catctagttt taaaaaagcg ttccaaacat tcaaaaatag
     4201 ttggaacttc ccttaataaa tccgtcactt tctttttcaa gatagcccaa gcttgctcaa
     4261 taggattcaa atccggtgaa taaggtggta gaggtaagaa aaagtgttta tcttggatgc
     4321 agagttcatc caaaatgttc ttgggatgaa aactagcatt gcccatgaca ataacatgag
     4381 gtgtcttcaa agcaggtagg agttgcgttt tgaaccactc cacaaagaaa tcgcttgtca
     4441 tgcttttctt gtaaatcatg ggagctataa actctccgtc tacttgtcct gcaacaattg
     4501 aagttcgctc aaaacgacgt ccgctaatct tttcatagac tttctcccct ctaggagccc
     4561 ctgcataagg acgatagagg tagcggtcga ttcctgtttc gtcaatatat acgattggta
     4621 aatcttttag gttatccaaa atatcaagaa actcagcgac tttctctggc tcttgttcct
     4681 taaagctcgt catctttttt taaagtgacc ttaatctgct ttaaagctgc ccatactgaa
     4741 ggaacagcac aatcaaaatg tgccgcaatt tcccgtaaaa aagcatctgg atgagcctct
     4801 acaaaggctt tcaattcctc taaaggaatc tttcggtttt tgacgactcg ctttttcctc
     4861 tctatgtgtc cttgttcaca tctttctttt cccacgtgaa gagagttctg acgccaacac
     4921 caaaaaactt ggctgcctcg acatggctgt gcccctcttt gatgtaatct aatgctcgct
     4981 gtttaaaatc agtactatat gccatagctt tattataata tgcttgttta aaactaagca
     5041 actatatcaa tgttttgtta ttagaagact tacttttttt actgaaatcg aatttttcct
     5101 aacctcaaca tcatataacc ccaaaattca atctgacctg gtttgaagag atgttcgaag
     5161 cagataaggt tttactaaaa ctaaaaaaaa tgatataatc aaactgatta aatgttttaa
     5221 aaataaagga gaatttgaat gtcttaccaa gaaaattacc agaaatgggt tgattttgtg
     5281 gagcttcctg actaccttcg tcaagatttg gaaaatatgg acgaaaaaac taaggaagat
     5341 gccttctata caaatcttga atttggtact gcaggtatgc gtggcttggt tggtgctggt
     5401 acaaaccgta tcaacatcta cgttgttcgc caagctactg aaggattggc tcgtttgatt
     5461 gagtcaaaag gtggaaacga gaaagaacgc ggtgtagcaa ttgcctacga tagccgtcac
     5521 ttctcacctg agtttgcctt tgaatctgcg gcagttcttg ctaaacacgg catcaaatct
     5581 tacgtatttg aaagccttcg tccgactcca gaactatcat ttgcagttcg tcatctcaac
     5641 tgtttcgcag gtatcatggt cacagccagc cacaaccctg ctccatttaa cggttacaag
     5701 gtttacggtg aagacggtgg acaaatgcct ccacacgatg cggacgcttt gactacttat
     5761 atccgtgcaa tcgaaaaccc atttgcagtt gaagttgctg atgtggaaac tgaaaaagct
     5821 tctggcttga ttgaagttat cggcgaagct gttgacgtag aataccttaa agaggttaag
     5881 gacgtaaaca tcaacccagc cttgattgaa gaatttggta aagacatgaa gattgtctac
     5941 acaccacttc atggtactgg tgaaatgttg gctcgtcgtg ctcttgccca agcaggattt
     6001 gactctgttc aagttgttga agcgcaagca actgctgacc cagacttctc aactgtaaca
     6061 tctccaaacc cagaaagcca agcagccttt gcccttgctg aagaacttgg tcgtcaagtt
     6121 ggtgcagatg ttcttgtcgc aactgaccca gacgctgacc gtgttggtgt tgaagttctt
     6181 caaaaagatg gtagctacct caacctttca ggtaaccaaa tcggtgctat catggctaaa
     6241 tacatcttgg aagctcacaa aaacgctgga actcttcctg aaaatgccgc tctctgcaaa
     6301 tctatcgttt caactgactt ggtaacgaag attgctgaaa gctacggcgc aactatgttc
     6361 aacgtcttga caggtttcaa atttatcgct gaaaaaatcc aagaattcga agaaaaacac
     6421 aaccacactt acatgatggg atttgaagaa agcttcggtt acttgattaa accatttgta
     6481 cgtgataaag atgccatcca agccgttctt gtcgttgctg aacttgctgc ctactaccgt
     6541 tctcgtggtt tgacacttgc tgacggtatc gaagaaatct ataaagaata tggctactac
     6601 gcagaaaaaa caatctctgt tactctttca ggtgtcgatg gtgctgaaca aatcaaagcg
     6661 attatggcta aattccgcaa caatgctcca acagaatgga acgcaacagc tatcactgtc
     6721 gtagaagact tcaaggcaca aactgctact gttgctgacg gtactgttac aaacttgaca
     6781 actcctccaa gtgatgtgtt gaaatacaca cttgctgacg gttcatggat tgccgttcgc
     6841 ccttcaggta cagaaccaaa aatcaagttc tacattgcag ttgtagggga aaccaacgaa
     6901 gaatcacaag ctaagattac taacatcgaa gcagaaatca atgcatttgt aaaataaaga
     6961 cctataaaag atgagaccaa ccaacctcat cttttttcgt atcaaatcta agcaatttta
     7021 gaaactttat ggcatactag acttatcaat gaaagcgagg taatctcatg gaacaatttt
     7081 tagataacat caaagacctt gaagtcacta cagttgtgcg tgcgcaagaa gctcttgata
     7141 aaaaagaaac tgcaaccttc tttatcggtc gcaaaacttg cccttactgc cgtaaatttg
     7201 caggtacatt gtcaggtgtc gtagctgaaa ccaaagctca catttacttc atcaatagtg
     7261 aagaaccaag ccaactcaat gatttgcaag cattccgctc acgttatgga atcccaactg
     7321 taccaggttt tattcatatt acagatggac aaatcaacgt ccgttgcgac tcttcaatgt
     7381 cagcacaaga aatcaaagat ttcgcaggat tgtaaaaaat cagctaacaa gtcttaaaat
     7441 cacaaaaacg cataatgtaa ggtgcaaaaa ccttgatatt atgcgtttta ttgttcctaa
     7501 aacagttaca tcagcatttc attacagaac attttaattt tggaagtcaa atcaattttt
     7561 aacaatgttt tagaaatcac agtgggctat tccatattca attatccaga ttcaatcgct
     7621 attatcgttt tcctagatta caaaaaaaac tacgttttgc taatcaaaac gtagtctcag
     7681 ccactatttt atcttactgt ccttctttta cttctttggt tgctacatct tctccaaacc
     7741 atttttggct gatttcttgg aacttgccgt ccttgtaaag actagaaaaa gcttcattta
     7801 tcttcttaac caaggttgta tcttccttac gggatccaac cgcaaaagct tctgtttcta
     7861 gtccaactgt aaagacatta taatcgttta aaacaccttc tgcttctaaa taatagtttg
     7921 catagacacg gtcaatcaat agaccatcaa ttcgatcgtt tttcaaatca atcaaggctt
     7981 cattaaaggt ttggtattga ttcgcttcct tattagcgac aatattcttc aaaatttctg
     8041 gatttgcttc aaagtccgca taaccagatg aaccagcttg agctcctaat gtctttccag
     8101 tcatatcctt tgcagtcgtg ataccagatg atttcttcgt aaccaatacc tgctcattct
     8161 tcatatatga gttactgaaa gccacctttt cacggcgttc gtctgtagcg gaatagccat
     8221 tccaaatcag atcaatcgtt ccttttgtca attcagcttc tttcaaatcc caatcaatcg
     8281 gttgccaatt taccgtgatt ccgtattttt caaaaacagc tgtagctaaa tcaatatcaa
     8341 atcctgcata agaaccatct ttctgagcaa atcccattgg aacaaaagta ctatcaaatc
     8401 caatagtaat agacttgtta gactggtact ttgaccaatt atctccacta gtttcgctag
     8461 aatttttccc acaagctact aagaacaaag cagtcatcag actgactaat acaagcatcc
     8521 attttttcat ccatttttcc tcctatttag gttctacttt caataataca tctgcgatat
     8581 tttcagcaaa ctgcaaatca tgggtaacca caatctgggt catcccaagt tccctatttt
     8641 gcaagattag cttctccact tccaaacgta attctggatc cagggcagaa gttggttcat
     8701 cgtagccaat gatttctggg tcaatcatca tagcacgcgc caaagccacc cgctgctttt
     8761 gcccaccaga tagtgagaaa ggataggcct ctgcgtgtcc tcctagtcct aactgttcca
     8821 agagcccact cgccttcttc tcagcctctt cctgcttcat tcccatggtc ttcacaggcg
     8881 ataaagtcaa attttccaga actgatagat gaggaaatag ttgaaaatct tggaagacaa
     8941 atcccagtag gttgcgcttc tgcaattcat ccagctctaa aggttgtcca ttataaaaga
     9001 tttgccctga atcaatggtt tcaagacctg caagcatacg taaaagagtt gtcttacctc
     9061 caccagaagg tccaacgata gccaggattt gcttttcagg aatacttaga ctgaaatcag
     9121 acaggatttg tttgtctcca aagactttat tgatatttcg taattctaac atagctgccc
     9181 cctatctata ataactatac ttcttctcaa cttttttgga aataattgtc acaatcccaa
     9241 ttaaaatcaa gtaaatggct cctgccaaga acataggaac cagactagca tcgcggttag
     9301 cagctgttcg actagccaag ataaggtctg aaattccgag agcatagacc aaagaagtat
     9361 ccttgaccaa actcataact tcattaaaga cactaggaag aacgatcttg gtcacttggg
     9421 gcaagataat atagcgcact ctgtcaaaag ggctaaactt caagaccttg gcagcctcat
     9481 actgtcctct tggaatagtg tcaattcccc cacggaaaat ttctgcaaag taagctgcat
     9541 agttgagaac aaaggcaata atagctgcag gaaggcggtc taaacgaatc ccaatacttg
     9601 ggagcacata atagataaaa atcagttgca agagtaaggg ggttcctcgc ataacccaga
     9661 tgtaaatgtt aatcagataa tggaggggct tccaatggac ttgcaaggca aaggcaatca
     9721 aaacgcccaa cggaatcgaa aatagcaaga ccagtgcaaa gacctgtaca gtcgtgctcg
     9781 caccattcag taaactcggt aatatctcaa ataaataaga catactgcac ctcctaaaag
     9841 taattgttcc tattatagca tgaataaaca agattaccaa gtatttttgt aaaaagattc
     9901 taccatatac aaataaaaac cgtagctatc aaaagagata aactacagtt ctatatgttt
     9961 aaacattctt acaatctctc aaacagttct tgcatttgca catcatctgg aaccagtttt
    10021 aagtaagtgt gagcatggac ttttgcttct tcaaaatgtc ccaattcacg caagagatag
    10081 atatagtgtt ccagaaattc tggattgtcc ttcaaatctc ctgtcaactc ttgataatgc
    10141 tcataagcag tatccaaatc gtccatttct tgataagaac gagcaatcat ccacttggtc
    10201 aaaagatttt ctggctcctc actctgcaag tctagaatat cctcataacg ctcctgctcc
    10261 agataaatag tggctaaacg aagcaagatt tcttctgtat cctcagcgtc ttcttttgca
    10321 gtaaggagat aattttctgc accactagca tcatgcaatt cataagaaaa ttgtgaagca
    10381 gctagcaaga ggcgagtttc aaagggattt ttctctaatc cttgcttagc gatacgcagg
    10441 gcttcttgaa cttgatgttc cttatgtaaa gcctgactgt acccatactc atagccttca
    10501 aagtcaggag aaatggtatc aagctgctta aagtagaggg tggctttttg atattcttct
    10561 tgatcaaaat aaagactggc caactcaaaa gctgttaagt catcgtattc taactccagg
    10621 gctttttcta aaaactcagt agccgtttca aatttcccta actgagcata ggcaaagcca
    10681 attcgttgat aggtggaaat gcccgtttgc tcataaatcg agcgattatc taactgggca
    10741 taggcttgaa tagccgcttg gtaattttcc aactcactat ccaactctgc caaacccaat
    10801 atcaagagag aatcctctga gtaggtcaag gcctccaata atttctcacg tgccacatct
    10861 gtcaaacctt ccagctggta aaggtctgcc ttcaaaacca aagacgagac ataccagtca
    10921 ctgtcagctt ggatttcctc aagataggta aaggcttctt ctatttgacc atcctcgcta
    10981 gcaattgcag ctagattaag atgaacctct ggaaaatcct ctacaatttt caggtaaatt
    11041 tccttggcct gaggatagaa accaatccct tcaagataag ttgccaactc atacagaaga
    11101 tcacttgaat cattttctaa agctttggcg aaataatgct cagccttagt taaatcttgc
    11161 tcctccaaag cctgtaacat ctgttgacta ttgttcactc ttgacttcct caccctctaa
    11221 ttcatataga ccactgacta ccttatacca ttcgaaaatg gctgaaatga caaccttagc
    11281 agaggcataa actggaatac caagtaaaac tccccagata ccaaacatag atcctgaagt
    11341 taacaaaaca aagagaacat taatagggtg gatgtttaat tgacttccca aaatcaatgg
    11401 agagacaaaa cggccttcaa tagtttgttc tacaataaag acaatcacta ctttcaaaag
    11461 catgactgga ccagcaatca aacccaatac tagggcagga agcatggcta gaaagctacc
    11521 aagataaggg accagattta aaataccagc agtaaccccc agcgtaaccg catagcgtag
    11581 accaataatc ttgaagaaga tgataaacat tactgctaca ataatagcca ctgtcacttg
    11641 ccctcgaaca tagttggaca actgttgatt cacatctgat agaacttgtc caacaggttc
    11701 cttcaatttt cttggaatga attgggtcaa atagttacgc aagcctttcc catcacgcaa
    11761 gagataaaag agcatgaaag gaacgataat caaggcaaca atcacttgag aagccccgct
    11821 aataaaggca ctcacccagt tgactgcctg agatgaaacc ttacttgcca aaactgtagc
    11881 ctggctagaa aaattggtca aaacttgctc taattgaggt ctgaaatcat ctggcagatg
    11941 ctgggctacc aatccattaa caatcctatc tatatcttct aagtaaacag gaacgtttct
    12001 tgcaaaggtc aaaacctgac gttgcagatt tggaatggcg actgccaagc cccaaatgat
    12061 aaagagagcg atgataacaa agacaatagt gatagctata acacgattaa ccttatgctt
    12121 ctccatccaa tcaacaatag gattcaacaa ataatataac aaaccagaca aaatgactgg
    12181 caacatcaca actgctaaaa agtctaaaac aggtgaaaat agaaaactaa tcttacttaa
    12241 aataaaaaga ttcaatccca ataataaggt tactaaaaat accgtaattg ccttgttatc
    12301 taaaaaccac ttgaaaaacc aagataggct aaaatgtttc tctttttgct ccataaatac
    12361 ttcctttcta ttgttctctc attataccat ttttgaaaca aaataactct tttttaaagt
    12421 gcttacatct aattggatgt ctttaatttg cttgtttttg tgatataata gtcttatctt
    12481 tacatagagg tatagtaatg aaagaaactg tttattttgg aacttataca cgtcgtactt
    12541 ctcaagggat ttacaaggca gactttgata cagaaactgg tcagctttca aatctagaac
    12601 tttttgcagc tgagccaagt ccaacctacc ttgcctttga ccagcaccaa catttataca
    12661 ctgttggtag ccaagacgat aaggggggaa ttgcagccta tcaaactgac gggactgtgt
    12721 taaatcatgt tgttgaagaa ggagctcccc actgttatgt tgctgtcgat gaaaagcgtg
    12781 atttggttta cgcagccaac tatcacaagg gacaagtcct tgtttataaa cgccaggaag
    12841 atggtagtct tctacttagt gatatggatc aacacagtgg ccaaggtcca catgaaaatc
    12901 aagcttcccc ccatgttcac tatacagatt taacacctga ccactatcta gtgacctgcg
    12961 acttgggaac tgaccaagtc atcacctatg acctcgatca agaaggaaaa ttatctaagc
    13021 tctataccta tcacagcaag ccaggagcag gctcacgcca tatcattttc cataaccact
    13081 ataaaatcgc ttatctcatt tgtgaactca atagtactat cgaagtttta atctacgatg
    13141 gcgttggcga atttgtacgt atgcaggtta tttcaacttt accagaagct tacgaaggct
    13201 ttaatggaac cgctgctatt catctctcta aagacggtaa atacctctac gcttctaacc
    13261 gtggccatga ttctatcgca gtatatacca tccttgcgga cggtagctta gagttattag
    13321 aaatcgttcc aacgcatggt cagactccac gtgattttga tttgacaccc gaccaaaaat
    13381 ttctcattgt tgtccatcaa gactctgaca atgcaactgt ctttaaacgt aattgtgaca
    13441 atggtcgtct agcagaactc tccaacgact tccatgttcc cgaagcagtc tgcatccgtt
    13501 ttgctcctta aaacaccata aaaaatgaac ttggtatttt caagttcatt ttttcttata
    13561 gtctatttcc gacattaata cggttaatgg cacgttgcaa agcaatctta gcacgacgtt
    13621 cttggtcaat caaatgtttg tcttgtgctt cttcaattgc acgttctgca cgaagtttgg
    13681 cacgttctgc acgactgata tcgatatcac gagcacgttc tgcagagtca gcgacgattg
    13741 taatcatatc attggcaatt tcaataacgc caccgtttac tgcaatccag ttcacgtgat
    13801 ctttatcatc gatacgtttt acctttactt catcaactgc taaaaccgca atcatatttt
    13861 catgtcgtgg caagatcccc atctcaccat ccagagttcg aaccgataca tagctggcat
    13921 ggtgatcata gacgagacca tctggtgtca cgatctggac agttaactga gccataggtc
    13981 acctcttaaa atcccatttt ttcagctttt gcaatcacat cttcgataga acctacacca
    14041 cggaaggcat cttctggcaa gtggtcgtat ttaccatcaa ggatttcctt aaagccacgt
    14101 acagtttcag caactggaac ataagaacct ggctgaccag taaattgttc cgcaacattg
    14161 aagttttgtg acaagaagaa ctggatacga cgggcgcgag caaccaaggt cttttcttca
    14221 tcagaaagct catccatacc aaggatagca atgatatctt gcaattcatg gtaacgttga
    14281 aggacacgtt ttacttcagc agcaactgca tagtgctctt ctccaacgat ttcaggtgcc
    14341 aaggcacgtg agcttgaagc aagtgggtca acggctgggt agatacccaa ttgtaccaat
    14401 ttacgttcca agtttgttgt tgaatccaag tgagcgaagg ctgttgctgg cgctgggtca
    14461 gtatagtcat ccgctggcac atagatagcc tggatagagg ttacagaacc cttcttggtt
    14521 gatgtgatac gttcttgcaa ttgacccatt tccgtagcaa gtgttggttg gtaaccaacg
    14581 gctgatggca tacgacccaa aagggcagat acttctgaac cagcctgagt gaaacggaag
    14641 atattatcga taaagagaag cacgtcttgg ccttccacat cacggaagta ttcagcgatt
    14701 gtcaaaccag taagggcaac acgcatacgt gctcctggtg gctcattcat ctgaccaaag
    14761 accatggctg ttttctcgat aacgcctgat tctttcattt cccagtaaag gtcattcccc
    14821 tcacgagtac gttccccaac accagtaaat actgaaatac caccgtgctc ttgggcaatg
    14881 ttgtgaatca attcttggat taagacggtt ttaccaactc cggcaccacc gaaaagtcca
    14941 actttaccac ctttaaggta aggggcaaga aggtcaataa ccttgatccc tgtttcaagg
    15001 atttcagaag aggtagacaa ctcatcaaaa gttggagctt ttttatgaat tggctgacgc
    15061 tctgcgtctt ctgtaaaagg agcttccaaa tcaatggtat ctcccaaaac gttgaagaca
    15121 cgtcccaaag tttctttacc tactggtaca gagattggac gacctgtgtc caatacttcc
    15181 attccacgag tcaacccatc tgttgattcc atggcgatag tacgaaccat accatctcct
    15241 aactccaagg ctacttcaag gacgattttt gtttttcttt cgtcattttt gtagacgaca
    15301 agtgcattgt taatctcagg aagtttttcc cctgctgcaa acaaaacgtc tacaacggga
    15361 ccgataacct gagcaatttt acctgaactc atctccttct cctattctat ataagatact
    15421 gtcggttcct agctcaacta ggtcctaagt tcattttcat acgagctgga ctagagccta
    15481 ttctaaggca ctagcacctg ctacgatttc tgtaatttct tgtgtaatcg ccgcctgtct
    15541 ggcacggtta tactgaattg tcaaatcatt gatgactttc ttagcattat ctgtcgctgt
    15601 ttgcatggct gtcatacccg cagcattctc agctgtcttg gcatcgataa tggcaccgta
    15661 aatcatactt tctgcaaact gaggcaacaa ctgctccaga atttcttctc ggctggtttc
    15721 caattcaaaa gtcaagctgt actcttcatc cgcttcattt ggatccaagt caacaatcgg
    15781 aagcatttgt tccacacgca tttgactggt tagcgtattg acatggtggt tgtagcaaac
    15841 ataaagctca tcaaagagtt cattttggta catttcaaca gtttttgaaa taatcttacg
    15901 aacttgatca aagctaggtt ggtctgccaa gccacgtaat tcataaagtg gttgaatacc
    15961 gcgagcctta aagaaatcag ctcccatccc accgatacag atcatttcaa aacctttacc
    16021 gtctgggtgg tattcttctt tcaactccat aacagctttc aaaatagagg aattataacc
    16081 tccaaccaaa ccgcggtctg aagtgataac gatatagcct gtcttcttca cagaacggct
    16141 aatcaacatc ggattagttg aagcaccagc tccattacca tgaaggatat ctgtcaaaag
    16201 tttacgcact ttctgagcgt aaacttggaa gttgcgagca gcttcttcag agcgacctaa
    16261 cttagcagcc gataccattt gcatagcatt agtgatttga ctcgtatttt ttgttgaggc
    16321 gatttttgtt ttaatatcat ttagagacac tgccatctga cacctctatt cttattggaa
    16381 gctagattga ttgagaaact ctgtaatcgc agcatccaag actgcttctt ctggcaagtc
    16441 ttttgtatca cgaatggttt ccaaaatctc tggatgttga gcatcaaaga aggcatggaa
    16501 ctcttcctcg aaacgaacaa tatcatctac tggaacagta tccaagaaac catgtgtcaa
    16561 agcataaaga atggttactt gtttctcaac aggtaatggt ttgtgaacag gttgtttcaa
    16621 gacctcaacg gtacgacgtc cacggttcaa cttagcctgt gttgctgcgt ccaagtcaga
    16681 accaaactta gtaaaggctt ccaactcacg gtatgaagca aggtcgatac gaagtgtacc
    16741 agcaaccttc ttcatggctt tgatttgtgc agaaccacct acacgagata cagatgaacc
    16801 cgcatcgatg gctggacgaa tacctgcatt gaagaggcca tcgccaagga agatttgtcc
    16861 atcagtgata gaaatcacgt tggttgcgat ataggctgag atatctcctg cttgtgtctc
    16921 gataaatggt agggctgtaa ttgatccacc accaagttca tcagaaactt tagctgagcg
    16981 ctcaagcaaa cggctgtgga gatagaaaac atcccctggg aaggcttcac gacctggagg
    17041 acgacgaagc aagagcgata gttcacgata agctaccgct tgttttgata gatcatcgta
    17101 tacaatcaaa acatgcttac cttgatacat aaattcttcc gccatagcaa ccccagtata
    17161 aggagctagg aagagcaatg gagatggttg tgaagcagag gctgtcacaa cgattgtgta
    17221 gtccaaggca ccgtactgac gaagtgtttc tacttgcgta cgaactgttg attctttttg
    17281 tccaatcgcg acgtagatac agatcatatc ttgatctttt tggttcaaga ttgtatcaat
    17341 cgcaatggtt gttttccctg tctgacggtc accgataatc aactcacgtt gaccacgacc
    17401 aatcggtaca agggcgtcaa tagctttcaa accagtttgc aatggttctg aaacagactt
    17461 acgttgcata acaccaggag ctggtgcttc tactggacga gttttatcag tgtggatttc
    17521 tccaagaccg tcaactggac gaccaagcgg atccacaaca cgaccaatca gactttcacc
    17581 tacagggact tccatgattt tccctgtacg gcggattgta tcgccttcac ggatatctgt
    17641 aaagtcacct aggatgataa taccaacgtc tgttgactcc aagttttgag ccataccata
    17701 agagccgttt tcaaaattca acaactctcc actcatgaca ttttcaaggc catgagcacg
    17761 cgcgataccg tccccgatat aggttacaac acctgtttca gtcacatcaa aattgggttt
    17821 gaaattttca atttgttgct taattaaagc gctgatttct tgtgcgttaa ttgccaaaag
    17881 aacaccactt tctatttcaa attttcttta acaactttaa gttgttgttt aatactcaca
    17941 tcaattgtct tgtgattggc aaaaatgaca aaaccaccaa tgagactttc atcgatttgt
    18001 tcttttacac tccttacttt cagagacatt tttttctcaa tcaaagggag caaacgagtc
    18061 ttctgttcat cagttagagg atgagcagac gtaatcgtca cttcaaatcg atttgtttct
    18121 ttttcaagtc ggttcaagca atctacaagc acatcataaa aaagatttgc tctgtgattg
    18181 taggccagaa cctggataaa gttttgtaat aaaggcgaca cagaatcttg gaaaaaagca
    18241 attgtttttt ccttatccga ctcgtctact gccacttgtt ttaaaaaaga aggcagacct
    18301 gttttttcaa caacttgctt gatttgagtc aagtctgaaa agatacggtc ttcttctcct
    18361 ttttcaagta ccaattggac aaaaggcatg ctgtattttt caattacctt tactgttttc
    18421 ttgtccatta agcttctcct agctgatcga tatactgatc aatgagtgct ttatgggcat
    18481 gactgtcaag gttttgtgag atgattttac cagctaagct gatggtcaaa tctgcgacct
    18541 cacccttaac actctgtaaa gcttctactt tattttgagc aatttcttga ttggcttttt
    18601 cttttaagtg tcctgcttct agtttagcat ctgctaagat attagcctta ctttgctcag
    18661 ctgtttcctt tgcattttca atgattgtct tagcttcttt acggctacca gccaattcat
    18721 cttcgcgttt ttgagccaat acttctgctt tttgacgggc ttcttcagct ctgtcaatat
    18781 ctgaagcaat tttttcagct ctttcttcga aaatgcctgt aatattagac catgcaaatt
    18841 ttttaatcaa gactagcaaa agaataaaag agccagtgat taaaacaaaa ttaccaatta
    18901 attcacctac tgttacgtgc atcagttact cctttctatt cctcttcaat attaatctta
    18961 ttccctaaat acacagatgt caaaagagta aaaacataag cttggatgca ggaaataaag
    19021 acagaaaatg cagtccaagc caaattagct ccaaaggcta ctggatacca ataaatagct
    19081 tggtgggaaa gaagaagtaa caaactcgtc atgacctctc ctgcaaagat attcccaaaa
    19141 atccgcaaag ccaaagataa gaagtttgta aattcttcca agatattcat cggtatgaca
    19201 aaaacaggac tcataaaaga ttttatactt tttttaaatc cacgacgacg aacgctttct
    19261 atatgtgtca acaaaatgac aagaaaagat aaggttaagt catactgtaa attagcggtt
    19321 ggcgaactcc accagttagt cccatcgatc gtttgaatct ttgtcattaa gccaaggtta
    19381 ttggcaatca ccatgaaaag gaataaacaa aggaaaaaga gtgagtaatc tttcatgtag
    19441 cgcgaaccaa tgttaggttc tgtaaatcca ataacaaagt catagacata ctcaagtaca
    19501 ttttgctttc ctttgggttt caaggtcata ttgcggcttg cccaataaat aaagacaaaa
    19561 ataactccca caatcaacaa agtcatggct aacatagtca gattgaagat aacaggacca
    19621 atagagatga ttggattaat actttcttcc atctaaaatc ctcccttttc ttctcgttcc
    19681 atgtatttat ttgataataa atgagaagac aagagttaca aagaaagttc cttcaataaa
    19741 ggcaacacct aaaaacatca aactacgaaa ctcagaaagc atatctggtt ggcgtgctac
    19801 tgatttaaac agtccattca tcaataaacc ttcaccgaca gatacgccca tacaggcaat
    19861 acataagcct aaaaatgtta aattcataac aaattctcct ttaaaataaa atttacttac
    19921 ttagtgtagt cctaaaattt aaatttgtca acttttaacc gctattggaa gcgtttaata
    19981 aaaattattt ccattttgct aaatttcaag ttcaagttag ccatccagaa gtcttctctg
    20041 ggtgacttgt agtccaagca ttttttagga tagttgttaa tccacttttc gatgaatgcg
    20101 acttctttgg gagtcatttt cttggttccc ttaggtaacc atctacgaat gagcctgttg
    20161 tgattctcat tagttcccct ttcccaagag gcatagggat gtgcataata aatgtgctcc
    20221 tcagaaaata cattagacaa gcgattgaat tccgttccat tatctgccgt gatggaaaga
    20281 atcttgtgtt gttttaagat gagttttaga gcctgattga ccacctcagc acttttattt
    20341 ggaatcaatc ggatgatctg atgtctactt tttcgatccg tcaagacaag caagcagtag
    20401 tttttcgctc tcgtaagtag aaccgtatca atctcataat gcccattctc caagcgaaaa
    20461 ttgatagctt caggccgctg ttcgatggat tgaccagcag gtttaaagtt ggtgctggcc
    20521 tgtttcttaa gcgcttttcc ttttctaggg taaagcagat cctgcttgct ttaccccaat
    20581 tttccatgat gaatccaata gtaaatggtt gaaattccca cgttaacccc tttagccata
    20641 accatcattt caggcaaaaa tttttggtta tgatagtgga gaatcttttc ctttagttcc
    20701 ttggtcacgc ttgatctctt gaccgagtgc ttgcgattat tttcataaga ctgttgagca
    20761 tagtcggcag aataaacctc tttgaagcgc ccttttccaa gacattttcg gactgtccta
    20821 cgcttgattt tagtgtggat agtttgagga gcttttccaa gtagagaggc aatttctcta
    20881 tttgattttc cttctttttt ccatctttcg attaagcgac ggctatcgat tgtcaaatgt
    20941 ttaccttttg tagtataatt gtcttgcatt tctgtgcctt tcttgtgttt gtggttgaac
    21001 aacaagtata acacagaggt gttttcttat gcctacaaga gctttcatta tttccatttt
    21061 cttttggatt tcactctatt ctgaaaaact tgtgtatatt tactgaagct agcaagtctt
    21121 acctgtaaat ttaatgaaag caacacaaaa tccgagaggg gaatctcgga ttaatagata
    21181 gagagttttt agtttaaata aattgtttaa aatatcaaca acatcacttc ttttcttaac
    21241 ctgataagtc ttgattccta attttggggc tacgattata ttgtcctcaa tatcgtctag
    21301 aaagacacaa tttctaggtt ataactggta tttatcaata gttactcata tacatcagtc
    21361 cacctccata cttatgtgcg agcctctctt tgtattatac ctccatactc accttacaga
    21421 ttcttttggt aataatatct ttgcctaatg tagagatagt cttgcaaaga aaaaacttcc
    21481 ttgtagccat gtttctgata aaagtccggt gcctggaact ggtaagtatt gacaaaggca
    21541 aaacaacaat ttcgattctt agcttcactt tctgcctgtt gcaatagttt tgaaccgatt
    21601 ccttgccctc gcagttcctc ttttacaaac aaatactcga tttctagcca atttccaaaa
    21661 gtctctgcta tcaaacctgc caggagattg cccttttcat cttcgacata aagattaagt
    21721 ggctcacttt cagcctcttc tctttttgaa cggttataaa cacgaatcag attccctatt
    21781 tcttgcgatt tatgtgattc cttattttcc aatctaaagt atagtgaaat gaaataaaac
    21841 atgcgcaaat cgattaagga atttaatcta atttctaaca atgtcttaga aatcaaagtg
    21901 tactatttta acttcaatgc actatacatc taatactcaa taaaaatcaa agagcaaact
    21961 aggaaactag ccgcaggttg ctcaaaacac tgttttgagg ttgtagatga aactgacgaa
    22021 gtcagtaacc atacatacgg caaggcgacg ctgacgtggt ttgaagagat tttcgaagag
    22081 tagcaaaatg gaaaaaggag tgagtgaagc acatcgcctc cccactcctt tttctgtttt
    22141 taggctgttt tttcaacctt caagattttt acatcatagc taccaacagg cgtttcaatg
    22201 gttgctgtat cacctgtttt cttgccaatc aaggcctgcc caattgggct ttcatttgaa
    22261 accttacctg caaaggcatc cgcaccagct gaacctacga taatataaac ttcttcttcg
    22321 tcctcaccaa tttcttggat ggtgactgtt ttaccaatcg ctacttcgtc ctgggcaact
    22381 gcgtcgctat tgacgatttc agcatagcgg atttttgttt ctaagctaga gatttgtcct
    22441 tcgacaaagg cttgttcatc cttagctgct tcgtactcac tgttttctga aaggtcaccg
    22501 tatgaacggg caatcttaat gcgttctacc acttctggtc gacgaaccaa tttcaattct
    22561 tctaattctt tttcaagttt ttccttttcc tcaagggtca taggatatgt tttttctgcc
    22621 atttttctca actttcttct gataatattt tctaaagaaa attatgtgaa gtatcacata
    22681 attttagttt gtttagttta atttgctgtt gacatgttca gcgacattgc ggtcgtggtc
    22741 ttcttgattg ttagcatagt agaccttgcc ttctgtgaca tctgctacaa agtagaggtt
    22801 atcgctctta gtttgattga tgcttgactc aatcgcatcc agacttggac tatcgactgg
    22861 accaggcatg agacctacat ttttataaac attataaggt gaatcaatgt tggtatcaat
    22921 cgcaacatcc tcagctagac tgatattttg ccccagtttt ccttgggcat acaagattgc
    22981 aatattactt tgaagtggca tatcacgatt caaacgattg tagaatacac ctgcaatgag
    23041 cttacgatct tctgtcttgg caccttcttt ttcgaccaag gaagcaatgg tcaacaactc
    23101 attgacagtc aagtttttag atttgatagt actatagtaa ggagataggt tcttatccat
    23161 agcagctaac atctcatcaa tcaagctctc aatagttgtg ctttccttga tagagtatgt
    23221 agctgggaaa aggtatcctt ccaaacgata acgcgcaccg ctgtctttta caggcaaact
    23281 ttccagtaaa gtaggatatt tcgctactgc ttgactgata aacgtctcat cttgaacttt
    23341 agccaagaaa gcctccgctg tcaaagactc tttgaagtca ccttgcaatt gacccacagt
    23401 ttgagcaatc tgatccaagg tataaccttc tggaattgtc aaagtcgcaa gtacaggttc
    23461 ttgcggttca tctgttccac ctttttgcaa ctctttgagt aagtcttctg tactcatact
    23521 cttttgcaaa ttgtagtaac ctgctttcaa gtcggtataa tttttatact tggcataaaa
    23581 actaaaaatc agaccatgct ttaccaaacc agctttttca agcgtcgtac cgatttcttg
    23641 aacgtttgaa ccttctggaa ttccaaccgt cacatatttc ttagaattag catcgatagg
    23701 taataaggaa tctagcacgt actggtaacc aaagtaacca cctgctgaga gcaaggcaag
    23761 gaatacaagg aatgaaatca caaaagcttt cacatgtgac ttcttctctt tcttaggctt
    23821 aacgggtttt gctccttctc tacggctacg acgtgatgta tctctgatga tatccactgt
    23881 ttctgtagca ggcgtaggtg tttctggacc tgcttgttct gcttttttct ctgccgtcgt
    23941 ataggaaaca gctacccttg ttggggtttc attgtattct ctttcaagtt tcttaggtct
    24001 aacaggacct ggacctggtc ttgaaccact ttcttccgct ggagaagaag gtacatcttg
    24061 acttggatga cttggaacac caggagtttc tctttgaatc tcatctgctg gagaagctgg
    24121 tacaccttga cttgggtgag taggcacggt aggagctttt ctcataatct cctctaccgt
    24181 tgacaaggaa tcagccatga gttcttcagc tgaaggttca tttgcaggag tgcgaactac
    24241 tgcctcatct tctttcagaa cttcatcata gccttttact ttttctaaat ctctcagaat
    24301 ctgctcttta aagcttaatt tctcttcttc tcttgacttt tcactcaaaa gtttttcctc
    24361 cttgttgata atccataata ttatatctta aaagtccaaa aaaagcaatc tatgatactt
    24421 ttcctaactt attttttcat ttcccagacc atatcatacc atgtttcccc tgcaaaggtt
    24481 gactgggact ggccttcgtt gacaaatcca tgtttttcat aataggcgat gagatagtca
    24541 tgacaggtta gattaatgcc atctctctca tcttcaagag caacttcttt caaggctgtc
    24601 agcaatttct gacccagtcc gagtccctgt gcctccttgg caatagacag acaggtcaca
    24661 gagatataac caccaggctc atgactatag tcttttattt cttctgtaaa agactggtct
    24721 tgcagatggc ggtgcaggcc aactggtcct tcgatataac ccatgattct tccttctttt
    24781 tcagcaacca gaaaagaggt ctgaatttct ctcaaatgtg cttcaaagac agaaggagga
    24841 atggcttctt cgaccgaaaa attttcaaat tcaagttcaa caatccgatc caaatcttct
    24901 aatcttgctt gtctgatttt cattgttcct ccagataaaa gggattaaac caaatcatac
    24961 tatagccctg gctagttgca tagagcaaaa tttcttcttc atcaacaaaa ccgttcattt
    25021 caaaatagga aagcagctca tcaggactct ccaaacgaat ccctttgtaa tccagctcaa
    25081 ctgccacctc tttcaaggct gcaagaagaa gtgttcccag gccctgtctc tgatggtcag
    25141 actcgatgac taaagaatgt acttttagac attgcggatt gtctgactgg ggacttgata
    25201 aaatatagcc taaaagttga ttttcatccc tagctagaag aaaggtatcc gcacacttac
    25261 ggatactttc ttctaaaata tgggaaagtt gctgcttttc agctggaaaa gacgaggtct
    25321 gaagtgcccc tatctcaggc aaatcaaact tgcttgcctg aatgatctta attggaattt
    25381 ccatgggaac atcctattga acattgcttg tcaagttaga caagagacgc tcaaatgagt
    25441 attcataggt ttggatgtct cctgctccca taaagacgta aacagcattg tcatggtcta
    25501 ggagtggaga aacattttca acagtaatca cttggtgttt tttgttgatt ttattggcta
    25561 ggtcttctac cttaacgtca ccatgatcta cttcacgagc cgagccataa atttgcgcta
    25621 gataaacagc atctgcttgg tttaaagcat gggcaaagtc gtccaacagg gcaatggttc
    25681 ttgtaaaggt atgcggttga aagactgcta caatttcctt gcttgggtat ttctgacgag
    25741 ccgcatccaa ggtcgcaata atttctgttg gatgatgggc aaagtcatca ataatcactg
    25801 tatcattgac aattttctca gtgaaacgac gtttaacacc ggcaaatgtt ttcaagtgct
    25861 cacgcaccaa gttcaaatca aatcctgctg tgtaaagaag accaataacg gctgtcgcat
    25921 tcatgatatt gtgacgacca aaggttggaa tgtggaattg ccccaagttt tgtccacgga
    25981 aatgaacggt gaaggttgaa ccagttgttg aacgaagaag atcactagct acaaagtcat
    26041 tgccttcagc ttcaaaacca taataataaa ttggtgcatc agacgtaatc ttacgcaatt
    26101 cagcatcttc accatagaca aaaagaccct tggtaatttg tttggcatag tcgttaaagg
    26161 cattgaaaac atcctcgaga cttgtgaaat aatctggatg gtcaaagtca atgttggtga
    26221 taatagagta ttctgggtgg taaggcatga agtgacgctc atattcgtca gattcaaaga
    26281 caaaatattt ggcattagcc gaaccacgac ctgtcccatc tccaatcaag aagctggtat
    26341 ctgtaatgtg agacaagaca tgagacaaca tacctgtcgt tgaagttttt ccatgtgctc
    26401 ctgctactcc catgctaaca aagtcacgca taaagctacc tagaaactca tggtaacgtt
    26461 tgtagctgat accattttgg tccgcatagg caatttcgac gttgttatct ggacgaaagg
    26521 catttccagc gataatttcc atatcaccgt ctagattctt ttcatcaaaa ggaagaatgg
    26581 taattcctgc ctgctcaaga ccgcgttggg taaagtagta cttttcaaca tctgatccct
    26641 gaaccttgtg ccccatctgg tgcaacatca aggccaaggc actcatccct gatcccttaa
    26701 ttccgataaa atgatatgtc tttgacatgt tttctcccct attctgtcat tctggtcaga
    26761 ttcaactctt gggcaacccg acgttcttgt tctgtttgtt tacttttttt attgtagatt
    26821 tggctcttct ttagaaaatc ataattgttt ttctttggag caggtgctga cacttcttca
    26881 ttcttggtag ggatagaatg aacttcttcc gccaagatat aatgagactg ggtcaatttt
    26941 tggctatatt tgacaaattc accaggattt tccttttgga aaggagttgt cggttgattg
    27001 ccctgtctaa ctagactggg ctgagaatga cgtctcgcaa ggctgaaatc ctgagttagg
    27061 tagttagcag agcgtttctt tttcaagtcc gcacgcgctt cttcacgcgc cacctccgca
    27121 tagctctttc cttctttttt aacccctaaa ggagcctttt taggtttttc gacttgcttt
    27181 tcaatcggtt ttactggttt ttcttcagta ataggagccc attctaaata atttttatct
    27241 cgatactcac ccttgatatt actgatcaga tcagactcat catagagatt catgactggc
    27301 atttcagtca acatgacctc gtcatctgac accaatggaa atcgttcttg tttcattttc
    27361 tatttccttt caacacttca ttatagcgta ttgtcttgat ttttcaagtg ctggcttcag
    27421 aaattcccaa aatttctcta atttctgcta gggtcagact accacgtgac tctgtgccat
    27481 ccaatacttg tgacaccaga tgtttctttt gttcttggag ttcctgaatt ttttcttcaa
    27541 tggttccctt ggtcaccaag cgatagacct caaccgtttc ttcctgaccc atccgatggg
    27601 cacggccaat ggcttgcgct tccaccgcag gattccacca aaggtcaacc aagatcactg
    27661 tatctgcacc tgtcaggttc agaccgaccc caccagcctt gagggaaatc agaaaggcat
    27721 ctctttctcc ttggttaaag gccttggtca tgtcttgtct ttccttggct ggggttgaac
    27781 ccgtaatttt aaaggatgtc aggcccaagt ctggcagttc ttgttcaatt ttttccaaca
    27841 ttcccttgaa ctgagagaaa atcaagacac ggtgtccgcc gtctgccacc tgtaccagta
    27901 gatctcggag actatctagt ttgccgctgg ctccctgata atcttccata aacagggcag
    27961 gagtgtcaca tatttgacgc aagcgcatca aaccagataa aatttccaca cgacttcgct
    28021 gaaattcctg ttctgacact tgagccagat ggtctcgcat ctgttgtaac tgggcaaggt
    28081 aaatagcctt ttgctggtct tccagttcat ttttataaac cacctcaatc aagtctggca
    28141 attcagtcag aacttcttct ttcttgcgtc gcatcacgaa aggcttgata aactgagcca
    28201 ctcgctctgc tggcaatttc ataaattctt tcttgcttgg caaaagtccg ggcatgacga
    28261 tttggaaaat agaccacaac tcacccagat ggttttcaat cggagttcct gacaaggcaa
    28321 agaccgacgg caccacaaat tgtctcaagg tctgggcaat cttggtctgg gcatttttca
    28381 tgacctgagc ctcatctaag aaaaggaagt caaaggccat cccctgataa aactcactgt
    28441 cctgacggaa ggtggcatag ctagtcacat agatttgatg gctctcggca agaatctcct
    28501 cacgacttgc tttcaaacca tgaacaacag ccacatccaa ctgtggggca aatttctgaa
    28561 actcatctgc ccagttgtaa atcaaacctg acggagcgag aatcaaaacc cgactttctt
    28621 ttgtcacttg actagtcaaa aaagcaatgg tctgaagggt tttcccaagt cccatatcat
    28681 cagccaaaat cccaccaaaa ccataatgat ggagcatctg caaccagcca attccctttt
    28741 cctgataatc tcgcaagtca gcctgaacct gagttgcctg tcgaggaaag tcctctggat
    28801 gtgtcaaatc ctgggccaga ttctggaatt cttgtgaaaa agaaacacgg tctcgccctt
    28861 caaagagatg agctaaactg taggccaagg atttccgagc ctgcaaggtc ccatctttta
    28921 attcaaattg ccccagttcc tgtagatttt ggcgaatttt cttggtttct tcatcgaaaa
    28981 agtaaacttg attagacgaa tcaatataaa aatcctgatt ggcaaccaag gcctgcatgg
    29041 cttggtcgat ttcctcctgg gcaatatctt gaaaatcaaa ctggatttcc aagagacctc
    29101 ccttggaggc aatctgcacc tgaggactcg ctaggctata aagctcttct agtttatctg
    29161 ataggtcaac atgcccgagt ttttcaaaga ctggaatgat atcatgaaaa aaatgataga
    29221 cagactccgc ttttaaggcc tgacgccaag attgaaaatc ggcttcaaag cccgcagcca
    29281 aacagacttg gaaaattctt tcttctaagt ccgcgtcact tgaaaatggt aattcttcta
    29341 gctcttgtcg gctagatacc tgtctatttc cataatcaaa ctgaatttct aaacaaatcc
    29401 gattatcttc ttccctgtca aagtaaaaag agggcgcaaa agttttgatt tgtagacgtt
    29461 ctggagctga aacggtgccc atctggataa aaagagtcag acaggaggcc aatttgtctc
    29521 gatcactgct atcaaattgc aggtatttct ttccttgttg acccacaggt aacgctttaa
    29581 tttccttgag aagacgcatc tgctggtctg ttaaaaaata aacctgacct ttatggaaaa
    29641 gtactgctcc ctgataaaag acattgaccc taggactctc actgatttcc atttcaaaat
    29701 agtccgagta ttctgttact gtaaaggcaa atagattggc atcagcatgc atatcctgaa
    29761 aaagcagggt ttggtagcta tccacttgat ggtcaaattg aaaatggggc aaggccatca
    29821 gtaaattcac accctgctca aaaaaggtca gagggaaaaa gaggtgccga ccttggtttt
    29881 ggaaaaagag gtctgaaacc agcccttcct ccgttagtcc gtgcaagaaa gtcaaaagtt
    29941 cttggctggc atcatcaaag gcttcccaag aaagagactc ctcataaatc ttgccaatca
    30001 tatacgactt tctctgctcg acaatcctta aaaaaagtgg aatatcccga atgacatagt
    30061 atttttggct attgatttgg ccgattctca gagtccacaa gatatgattg gttcctgctt
    30121 ccacctgacc cacagctgat aactcatagg cgcattctga ttttggagat aaaattcgat
    30181 ccaaaaactt gccacccaag gtcaccttgg tttcaacagc ctctttttct tcatgacctt
    30241 cttccagact ccacaagatt tcctgaccac gctcatcatt tttcagaaaa tgctctagcg
    30301 ctgccaaatg cacacagtaa cccctctttt gaaaaaaatc acaggcacaa aaaaccaaat
    30361 catcctctaa actatagcgc agttcttctt ctgcaacgcg agcgtagagc cgattgttct
    30421 tttccttgat gatatcaacc ttaccagttt cataaagggc aacaccttcg atacgaattt
    30481 tccccggaat caatttagcc atattttcac ctttacctta tctttttatt ataccatatt
    30541 ttcgcctatg aaaatagcct tctaggaaga cttttctcct agaaggctgg atttttaacg
    30601 tttggcaaaa gtagccacaa tccgctgaca gacttcttgc aacagagatt tgggcatagc
    30661 tatattgatg cgggcatgga gacttccttc ctctccaaaa tccaaaccac ggttgaggat
    30721 aaccttggct tcatttctca acaactcttg caatgtttca tcagtcaggt cataagctga
    30781 aaagtcaagc caaatcaagt aggtaccttg cggtttcatg accttgattt tagtctcttt
    30841 tccaaataga tccaccacat aattgatgtg gtcttcaaag acttgcttga gttcctctag
    30901 ccaatcttta ccgtatcgat aggcagcttc tgtcgccaaa taacccaagc ctgaaatttc
    30961 atgctgatta ttggccaaca ggcgtttctg gaaagctagt ctcaacttag gattttcaat
    31021 gactgcatag gaattttttg ttccagcaat attaaatgtt ttagtggcac tgctcaagac
    31081 gatagcaaaa tttttgaagg caggattgat ggtattgaaa gactggtgtt tgtgaccaaa
    31141 gagggtcaaa tcttggtgaa tctcatccga aactaacaaa acaccgtgtt tttggcagag
    31201 ttggccaatc ttctccaaca cttctttttc ccaaacacgt ccaccaggat tgtgagggtt
    31261 gcaaagaata tagagtttaa cctcctcttc caccaaatcc ttttcaagtt ggtcaaagtc
    31321 aatctcaaac agaccatcct tttccactaa ggaattagta atcaatctac gattattcaa
    31381 cttgacactg cgagcaaagg gtgggtagac aggcgtgtta attaaaaccg cctcgccttc
    31441 ttttgtaaag gtttgaatag ctgttgagat ggctggtacc acaccctcga taaagacaag
    31501 agcctctttg tcaaagttgt aaccgtattg tgtagcttcc cacttttgaa cttccttaat
    31561 taagtcttca ctggcatagg tataaccata aaccagttgg tctgcgtaag tttgcacggc
    31621 ttggcggatt tcaggcaaga ccacaaagtc catatccgct atccaagctg gtagaacttc
    31681 actatccgtt tctgtttctt tccatttata ggtatggtgc cctaaacggt tgggcaggct
    31741 tgtaaaatca tattttccca tctttgtctt atccttctat ggcttggcgc aaatctgcaa
    31801 tcaaatctct agcatcctca atcccaatag acaaacgcaa gaggtcatct gtcaaaccat
    31861 aagaatggcg tacctctgct ggaatatcag catgagtttg agtcgttgga taagtaataa
    31921 gactttccac tccacccaaa ctttccgcaa aagagaagac cttgagactg ttcaaaatat
    31981 gaggaatgcg tgtttcatcg gctactttaa aggaaatcat gcctccacga ccagtgtaga
    32041 gaacttcctt aactgctgga gaatccttca aaaaggcaac cacttcttgg gcgttagctg
    32101 ttgagcgctc catacgaaga gacaaggtct tgagaccacg aagcaactgg tagctgtcaa
    32161 atggagacaa gactgcccct gttgtattaa gattgtaaaa aagcttctcg tatagttcta
    32221 aactattggt cacaaccact ccagccaaga catcattgtg gcctgctaga tacttggttg
    32281 ctgaatggag aacgatatct gctccatctt caatcggacg ttggtagata gggctataga
    32341 aggtattgtc caccaccact ttggcaccct tagcatgagc caattttgct agtttttcga
    32401 tatcaaattc caacatcaag ggattggttg gggtttcgat atagagaaca tccacatcct
    32461 tttctaactc ggcaatcaac tcttcttctg tattggcata ggtaaaatgg aaacgacctt
    32521 cctgctccac ttggttaaac cagcgaaaag aaccgccgta aagatcacgc actgccaaga
    32581 ccttacttcc tactggaaag ccgctaaagg ccagtacaat agctgacatc cctgagctag
    32641 tcgctaaggc atagtctgct gactcaatag tcgccaagac ttcctcagcc ttactacgag
    32701 ttggattttt agtgcgcgta tagtcaaacc cagtagatcg accaaactct ggatgctgat
    32761 aggtcgttga aaaatgaagc ggtgtcacca atgcacctgt cgcttcatct gacttaatac
    32821 ccgcctgggc caaaattgtg ttaatgtgta attccttgct catacaatac ctccaaatct
    32881 atagtaacta ttgtaccact tattttcatc aactcatttt cttcttttca agagctagtt
    32941 atagtttcaa actatataaa aagggagttt ttcctgctcc ctttaataga ctataaaatg
    33001 gtgaatctca aaagacactt cactctatca tttgctcctg cacaaaacga gcataacgct
    33061 catgattttc cagtagttcc ttatgagttc ctgagccagt gattttcccc tcctctaaga
    33121 agaaaataca atccacatct tttaccgttg acaaactatg cgctataatc acaaccgtct
    33181 tctcctttaa tacagaatag aggctactga taatcgcata ctcagaatcc gcatcaagat
    33241 tagcagtagc ttcatcaaat ataagaattt cagcatcttt taagtaggtt ctagctattt
    33301 gaagtctttg gctttgcccc cctgacaaga atcgtccgcg ttcaccaact tcagtatcta
    33361 gtccctcttt catggagcga atctcatcac ctagtgatac taagtctagc actttcatca
    33421 attcatcatc agttactaag cgattcaaac cgagacaaag attgtcacga atactgccag
    33481 ataagactgc attattttgt gaaacccaag cgattttact tctccattct tttaagttaa
    33541 aatcatatat acttgattgc tccattagaa tatctcctga aagcggttta taaaaccgct
    33601 ctaacaaacg cacaatcgtt gattttcctg atccagatgg tccaacaaaa gcaatttttg
    33661 ccccttgaaa attgaacaag taatatcctt taagacaggt cgattttcat cataaccaaa
    33721 atagacatgg ttaaaattca acccttgtcc tgataccgat tttcctccct caaatttttc
    33781 tttaggaact gcaagcaagt tatccagtgc aactgaagat cccttgctcc tagaataaac
    33841 agttacaaaa ttagctatat tactaatagg attaagtaat tgaaagaggt aaatcaaaaa
    33901 cgaaaccaag gttcccacag atatatatcc tgcgctgacc cgataacccc cataggttag
    33961 catcacagct atagtcgcaa agataaataa gagagcaaac ggggtctcaa aagaagtaac
    34021 cctatctgat ttcagtgaat tgttttgtac cctttcaata caattatcca aaacatcctg
    34081 tacacttttc tctgcttggt tagtcttaat taattcatgt tcttgaatct tttcagtcaa
    34141 ttgccctgtt aaatttcctc ctgtaaacga cgactatact tttcactgat attggaaagg
    34201 ggcaagataa taaacatcat acaaggaaga gtgatgaata aaagtagaga aagattccaa
    34261 tcaagactaa ataagactac aatggaacca agtaccataa ctaaactcag aataatattt
    34321 gggaaagtcg taattaaaaa ctcacgaatg acactcatgt cattgacaat ggcagaagtc
    34381 aactccccac tttggctctt atcaaagaag gatttctctg cataaatcaa cccct
//
LOCUS       DBERMR010000018        34425 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.018, whole genome shotgun sequence.
ACCESSION   DBERMR010000018 DBERMR010000000
VERSION     DBERMR010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 34425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34425
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.018"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            593..1045
                     /locus_tag="ACL8A1_000949"
     CDS             593..1045
                     /locus_tag="ACL8A1_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HGS1201738.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            1064..2401
                     /locus_tag="ACL8A1_000950"
     CDS             1064..2401
                     /locus_tag="ACL8A1_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS1201739.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIRGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2480..3364
                     /locus_tag="ACL8A1_000951"
     CDS             2480..3364
                     /locus_tag="ACL8A1_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS1201740.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3364..4197
                     /locus_tag="ACL8A1_000952"
     CDS             3364..4197
                     /locus_tag="ACL8A1_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS1201741.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAVFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4215..6308
                     /gene="nanB"
                     /locus_tag="ACL8A1_000953"
     CDS             4215..6308
                     /gene="nanB"
                     /locus_tag="ACL8A1_000953"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HGS1201742.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISSLLLDKLSGESQTVVMKFKADEPNSLQALFGLSNSKADFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLREKHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSEVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6320..7423
                     /locus_tag="ACL8A1_000954"
     CDS             6320..7423
                     /locus_tag="ACL8A1_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGS1201743.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDVCKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVRLSEIVK"
     gene            7597..8295
                     /locus_tag="ACL8A1_000955"
     CDS             7597..8295
                     /locus_tag="ACL8A1_000955"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HGS1201744.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8314..9831
                     /locus_tag="ACL8A1_000956"
     CDS             8314..9831
                     /locus_tag="ACL8A1_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGS1201745.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADPAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPFGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            9956..11284
                     /locus_tag="ACL8A1_000957"
     CDS             9956..11284
                     /locus_tag="ACL8A1_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS1201746.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11536..12402
                     /locus_tag="ACL8A1_000958"
     CDS             11536..12402
                     /locus_tag="ACL8A1_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS1201747.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            12418..13257
                     /locus_tag="ACL8A1_000959"
     CDS             12418..13257
                     /locus_tag="ACL8A1_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS1201748.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13530..13982
                     /locus_tag="ACL8A1_000960"
     CDS             13530..13982
                     /locus_tag="ACL8A1_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HGS1201749.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNQFEHHKNYADLHLLIEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            14079..14423
                     /locus_tag="ACL8A1_000961"
     CDS             14079..14423
                     /locus_tag="ACL8A1_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201750.1"
                     /translation="MVSSKYAARASFLDGQGITVDEMSWIINGIVNGLIGRYIKLGIY
                     AAKYGISMVRSILSRAAATAAARVGLLTKIFGWILRIAVLAADVYGNFANNIAVAWDA
                     HDKIPNNGRINF"
     gene            14433..14645
                     /locus_tag="ACL8A1_000962"
     CDS             14433..14645
                     /locus_tag="ACL8A1_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201751.1"
                     /translation="MKALCIFLLNMLAWTVLAMTVRGGLDGFNEHTWSTILIASLSGI
                     YIDYMSIDKNRKKSKRKNRFVHGRDL"
     gene            14703..15347
                     /locus_tag="ACL8A1_000963"
     CDS             14703..15347
                     /locus_tag="ACL8A1_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HGS1201752.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            15516..16433
                     /locus_tag="ACL8A1_000964"
     CDS             15516..16433
                     /locus_tag="ACL8A1_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HGS1201753.1"
                     /translation="MSDLKKYEGVIPAFYACYDDQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGARAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEVLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            16451..17335
                     /locus_tag="ACL8A1_000965"
     CDS             16451..17335
                     /locus_tag="ACL8A1_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGS1201754.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17351..18202)
                     /locus_tag="ACL8A1_000966"
     CDS             complement(17351..18202)
                     /locus_tag="ACL8A1_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HGS1201755.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18402..20459
                     /gene="pbp2b"
                     /locus_tag="ACL8A1_000967"
     CDS             18402..20459
                     /gene="pbp2b"
                     /locus_tag="ACL8A1_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HGS1201756.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            20470..21066
                     /gene="recR"
                     /locus_tag="ACL8A1_000968"
     CDS             20470..21066
                     /gene="recR"
                     /locus_tag="ACL8A1_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HGS1201757.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21242..22285
                     /locus_tag="ACL8A1_000969"
     CDS             21242..22285
                     /locus_tag="ACL8A1_000969"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HGS1201758.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            22369..23742
                     /gene="murF"
                     /locus_tag="ACL8A1_000970"
     CDS             22369..23742
                     /gene="murF"
                     /locus_tag="ACL8A1_000970"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HGS1201759.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIKTAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIHQAFQGLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSIQLHNQMILSLSPDVLDTVIFYGEDIAE
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLAKQVKESLGAYDQILLKGSNSMNLAMLVE
                     SLENETK"
     gene            23729..24340
                     /locus_tag="ACL8A1_000971"
     CDS             23729..24340
                     /locus_tag="ACL8A1_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HGS1201760.1"
                     /translation="MKPSDFVKYLQRMIALTDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQGSDLDAEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIEILWQVYQGQREQYLD"
     gene            24431..25135
                     /locus_tag="ACL8A1_000972"
     CDS             24431..25135
                     /locus_tag="ACL8A1_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HGS1201761.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            25354..26727
                     /gene="ftsA"
                     /locus_tag="ACL8A1_000973"
     CDS             25354..26727
                     /gene="ftsA"
                     /locus_tag="ACL8A1_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HGS1201762.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDTAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26744..28003
                     /gene="ftsZ"
                     /locus_tag="ACL8A1_000974"
     CDS             26744..28003
                     /gene="ftsZ"
                     /locus_tag="ACL8A1_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HGS1201763.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETAELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            28008..28679
                     /locus_tag="ACL8A1_000975"
     CDS             28008..28679
                     /locus_tag="ACL8A1_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HGS1201764.1"
                     /translation="MNVKENTELVFREVAEASLSANRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELAGLDKIEYVGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNIPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            28689..29228
                     /locus_tag="ACL8A1_000976"
     CDS             28689..29228
                     /locus_tag="ACL8A1_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HGS1201765.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTSVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            29228..29491
                     /locus_tag="ACL8A1_000977"
     CDS             29228..29491
                     /locus_tag="ACL8A1_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HGS1201766.1"
                     /translation="MIFLIRMIYNAVDIYSLILIAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            29488..30273
                     /locus_tag="ACL8A1_000978"
     CDS             29488..30273
                     /locus_tag="ACL8A1_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HGS1201767.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            30282..31070
                     /gene="divIVA"
                     /locus_tag="ACL8A1_000979"
     CDS             30282..31070
                     /gene="divIVA"
                     /locus_tag="ACL8A1_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HGS1201768.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMAELQARIEV
                     ADKELSEFEAQIKQEVEAPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            31418..34210
                     /gene="ileS"
                     /locus_tag="ACL8A1_000980"
     CDS             31418..34210
                     /gene="ileS"
                     /locus_tag="ACL8A1_000980"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HGS1201769.1"
                     /translation="MKLKDTLNLGKTEFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGEARKFVVAAELLTSLSEK
                     FGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMVAETIEHVAQLFEEHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPELTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDTVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFETEDFVQLSELPEAQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SDLTIAEGPAPEAALSFEDVAFTVERAAGEVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFADAVAEGFEEK"
BASE COUNT        10601 a         6080 c         7478 g        10266 t
ORIGIN      
        1 aaaaaaaaaa agtatactct aattgaaaac ggtaacaaaa ctaatttaga gaatgaaata
       61 tagagtattt ctctcttaaa agtttttggt gaaacgagat gtagaaagga gatttagcca
      121 aagagtctat tagtgctaga ataatagatt agaattattt tagaaaaacg aagtgagcag
      181 cttataaatt caagtcccca aatagattca tactagtatc ttttgcaaaa aataaagggc
      241 gacttccttc atgaatatca atttcatcta taaggaaggt agctaattga actaacttat
      301 ttattctgtt tgtcgctaga aaaatcagac ctccttgtga agattgagga gatactgaat
      361 gaaaatcaaa gaagaaacta gcaagctagt agcagattgc ccaaaacacc gctttgaggt
      421 tgtagataag actgacgtat ataatccaag gtgaagcgac tgtggtttga agagattttc
      481 aaagagtata ggctagagag tagtgttttt atgtccttct agtagaaaat gctagacaga
      541 agaatgggga acttggatag gagaaataga ttgagaaagg aggttagaag agatgattat
      601 tacaaaaatt agccgtttag gaacttatgt gggagtaaat ccacattttg caacattaat
      661 agattttcta gaaaaaacag gactagaaaa tttaacagaa ggttcgattg ctatcgatgg
      721 taatcgattg tttgggaatt gctttactta tctagcagat ggtcaagcag gggctttctt
      781 tgaaacacac caaaaatatt tggatattca tttagttttg gaaaacgaag aagccatggc
      841 tgttacatcg ccggaaaatg taagcgttac ccaagaatat gatgaagaga aagatattga
      901 attatacaca gggaaagtgg aacagttggt tcatttgaga gctggtgaat gcctcatcac
      961 ttttccagaa gatttacatc aacccaaggt tcgtataaat gatgaacctg tgaaaaaagt
     1021 tgtctttaaa gttgcgattt cttaatgtag aaagagaaga acgatgaaaa aaatgagaaa
     1081 gtttttatgt ctagctggaa ttgcgctagc ggctgttgcc ttggtagctt gttcaggaaa
     1141 aaaagaagct acaactagta ctgaaccacc aacagaatta tctggtgaga ttacaatgtg
     1201 gcactccttt actcaaggac cccgtttaga aagtattcaa aaatcagcag atgctttcat
     1261 gcaaaagcat ccaaaaacga aaatcaagat tgaaacattt tcttggaatg acttctatac
     1321 taaatggact acaggtttag caaatggaaa tgtgccagat atcagtacag ctcttcctaa
     1381 ccaagtaatg gaaatggtca actcagatgc tttggttccg ctaaatgatt ctatcaagcg
     1441 tattggacaa gataaattta acgaaactgc cttaaatgaa gcaaaaatcg gagatgatta
     1501 ctactctgtt cctctttatt cacatgcaca agtcatgtgg gttagaacag atttgttaaa
     1561 agaacataat attgaggttc ctaaaacttg ggatcaactc tatgaagctt ctaaaaaatt
     1621 gaaagaagct ggagtttatg gcttgtctgt tccgtttgga acaaatgact taatggcaac
     1681 acgtttcttg aacttctacg tacgtagtgg tggaggaagc ctcttaacaa aagatcttaa
     1741 agcagacttg acaagccaac ttgctcaaga tggtattaaa tactgggtta aattgtataa
     1801 agaaatctca cctcaagatt ctttgaactt taatgtcctt caacaagcta ccttgttcta
     1861 tcaaggaaaa acagcatttg actttaactc tggcttccat atcggaggaa ttaatgccaa
     1921 cagtcctcaa ttgattgatt cgattgatgc ttatcctatt ccaaaaatca aagagtctga
     1981 taaagaccaa ggaattgaaa cctcaaacat tccaatggtt gtttggaaaa attcaaaaca
     2041 tccagaagtt gctaaagcat tcttagaagc actttataat gaagaagact acgttaaatt
     2101 ccttgattca actccagtag gtatgttgcc aactattagg gggattagcg attctgcagc
     2161 ctataaagaa aatgaaactc gtaagaaatt taaacatgct gaagaagtaa ttactgaagc
     2221 tgttaaaaaa ggtactgcta ttggttatga aaatggacca agtgtacaag ctggtatgtt
     2281 gactaaccaa cacattattg aacaaatgtt ccaagatatc attacaaatg gaacagatcc
     2341 tatgaaagca gcaaaagaag cagaaaaaca attaaatgat ttatttgagg ctgttcagta
     2401 gatgtaaaag actagaaaat aggtgggcta gtgagctgaa aagctctagc ccaatcttgt
     2461 aaaagaaggg agaaggagaa tggttaaaga acgtaattta actcgctgga tatttgtttt
     2521 gccagctatg attatcgtag gattactctt tgtttatccg tttttctcga gtatttttta
     2581 tagctttacc aataagcatt tgattatgcc taattataaa tttgttggtt tggctaacta
     2641 taaagctgtg ctatcagatc ccaacttctt taatgcgttc tttaattcaa ttaagtggac
     2701 cgttttctca ttagttggtc aagttttagt agggtttgtg ttggctttag ctcttcacag
     2761 agtacgccac ttcaagaaat tatataggac cttattgatt gttccttggg catttcctac
     2821 catcgttatt gccttctctt ggcagtggat tctaaacggg gtttatggct acttacctaa
     2881 tctaatcgta aaattaggtt taatggaaca tacacctgca tttttgacag atagtacatg
     2941 ggcattccta tgtttggtgt ttatcaacat ttggtttgga gcaccaatga ttatggttaa
     3001 tgtgctttca gctttgcaaa cagtaccaga agaacaattt gaggctgcta agatagatgg
     3061 tgcttcaagt tggcaggtgt tcaagtttat cgtctttcca catattaaag tggttgtagg
     3121 acttctagtt gttttgagaa ctgtatggat ctttaataac tttgacatta tctacctcat
     3181 tactggtggt ggaccagcca atgctacaac gacgcttcca atttttgctt acaacctggg
     3241 ctggggaact aaattgttgg gtcgtgcttc agcagttaca gtattgctct ttatcttctt
     3301 ggtggcgatt tgctttatct actttgctat catcagtaag tgggaaaagg agggtagaaa
     3361 ataatgaaga agaaatccag tatttattta gatattctct cacatgtact tttagttggt
     3421 gcgaccatcg ttgcagtttt cccattggta tggattatca tatcttctgt caaagggaaa
     3481 ggggaattaa ctcagtatcc aacacgattt tggcctgaac agtttacatt agattatttc
     3541 actcatgtta tcaacgattt gcacttcatt gataacattc gaaacagttt aatcattgcc
     3601 ttggctacaa ccctcattgc gattattatt tctgctatgg cagcctatgg tattgttcga
     3661 ttctttccta aattgggagc aatcatgtcg agactactcg tcattaccta tattttccca
     3721 ccaattttgt tagcaattcc ctattcaatt gccattgcta aagttgggtt aacaaatagt
     3781 ttatttggct tgatgatggt ttatctatct tttagtgttc catatgcagt ttggctctta
     3841 gttggatttt tccaaacagt tccaattgga attgaagaag cggctagaat tgatggtgca
     3901 aataaatttg ttacgtttta taaagttgtg ctaccgattg tagcaccagg tattgtagca
     3961 acagctattt atacatttat caatgcttgg aatgaattcc tgtatgcctt gattttgatt
     4021 aacaatacag gaaagatgac agtagcagta gcccttcgtt cacttaatgg ttcagaaata
     4081 ctagactggg gagatatgat ggcagcgtct gttattgtag ttcttccatc aattattttc
     4141 ttctctatca tccaaaataa gattgcaagt ggattatcag aaggatctgt gaagtagacg
     4201 aaagaaggaa aaaaatgaat aaaagaggtc tttattcaaa actaggaatt tctgttgtag
     4261 gcattagtct tttaatggga gttcccactt tgattcatgc gaatgaatta aactatggtc
     4321 aactgtccat atctcctatt tttcaaggag gttcatatca actgaacaat aagagtatag
     4381 atatcagctc tttgttatta gataaattgt ctggagagag tcagacagta gtaatgaaat
     4441 ttaaagcaga tgaaccaaac tctcttcaag ctttgtttgg cctatctaat agtaaagcag
     4501 actttaaaaa taattacttt tcaattttca tgagagattc tggtgagata ggtgtagaaa
     4561 taagagacgc ccaaaaggga ataaattatt tattttccag accagcttca ttatggggaa
     4621 aacataaagg acaggcagtt gaaaatacac tagtatttgt atctgattct aaagataaaa
     4681 catacacaat gtatgttaat ggaatagaag tgttctctga aacagttgat acatttttgc
     4741 caatttcaaa tataaatggt atagataagg caacactagg agctgttaat cgtgaaggta
     4801 aggaacatta cctcgcaaaa ggaagtattg atgaaatcag tctatttaac aaagcaatta
     4861 gtgatcagga agtttcaaat attcccttgt caaatccatt tcagttaatt ttccaatcag
     4921 gagattctac tcaagctaac tattttagaa taccgacact atatacatta agtagtggaa
     4981 gagttctatc aagtattgat gcacgttatg gtgggactca tgattctaaa agtaagatta
     5041 atattgccac ttcttatagt gatgataatg ggaaaacgtg gagtgagcca atttttgcta
     5101 tgaagtttaa tgactatgag gagcagttag tttactggcc acgagataat aaattaaaga
     5161 atagtcaaat tagtggaagt gcttcattca tagattcatc cattgttgaa gataaaaaat
     5221 ctgggaaaac gatattacta gctgatgtta tgcctgcggg tattggaaat aataatgcaa
     5281 ataaagccga ctcaggtttt aaagaaataa atggtcatta ttatttaaaa ctaaagaaga
     5341 atggagataa cgatttccgt tatacagtta gagaaaatgg tgtcgtttat gatgaaacaa
     5401 ctaataaacc tacaaattat actataaatg ataagtatga agttttggag ggaggaaagt
     5461 ctttaacagt cgaacaatat tcggttgatt ttgatagtgg ctctttaaga gaaaaacata
     5521 atggaaaaca ggttcctatg aatgttttct acaaagattc gttatttaaa gtgactccta
     5581 ctaattatat agcaatgaca actagtcaga atagaggaga gagttgggaa caatttaagt
     5641 tgttgcctcc gttcttagga gaaaaacata atggaactta cttgtgtcct ggacaaggtt
     5701 tagcattaaa atcaagtaac agattgattt ttgcaacata tactagtgga gaactaacct
     5761 atctcatttc tgatgatagt ggtcaaacat ggaagaaatc ctcagcttca attccgttta
     5821 aaaatgcaac agcagaagca caaatggttg aactgagaga tggtgtgatt agaacattct
     5881 ttagaaccac tacaggtaag atagcttata tgactagtag agattctgga gaaacatggt
     5941 cggaagtttc gtatattgat ggaatccaac aaacttcata tggcacacaa gtatctgcaa
     6001 ttaaatactc tcaattaatt gatggaaaag aagcagtcat tttgagtaca ccaaattcta
     6061 gaagtggccg taagggaggt caattagttg tcggtttagt caataaagaa gatgatagta
     6121 ttgattggaa ataccactat gatattgatt tgccttcgta tggttatgcc tattctgcga
     6181 ttacagaatt gccaaatcat cacataggtg tactgtttga aaaatatgat tcgtggtcga
     6241 gaaatgaatt gcatttaagc aatgtagttc agtatataga tttggaaatt aatgatttaa
     6301 caaaataaag gagaaaaaca tggttaaata cggtgttgtt ggagcagggt attttggagc
     6361 tgaattggct cgctacatgc aaaaaaatga tggagcagag attactcttc tctatgatcc
     6421 agataatgca gaggcgattg cagaagaatt gggagcaaaa gtagcaagtt ccttagatga
     6481 gttggtttct agcgatgaag tagactgtgt tatcgtcgca actccaaata atcttcataa
     6541 ggaaccggtt attaaggctg cacagcatgg taaaaatgtt ttctgtgaaa aaccaattgc
     6601 gctttcttat caagattgtc gcgagatggt agatgtgtgt aaagaaaaca atgtaacctt
     6661 tatggcagga catattatga atttctttaa tggtgttcat catgcaaaag aactcattaa
     6721 tcaaggagtt atcggagacg ttctatattg tcatacagct cgtaatggtt gggaagaaca
     6781 acaaccgtca gtatcatgga aaaaaattcg tgaaaaatca ggtggtcact tgtatcacca
     6841 catccatgaa ttggattgcg ttcaattcct tatggggggc atgcctgaaa ctgtaaccat
     6901 gacaggtgga aatgtggccc atgaaggtga acatttcggt gatgaagatg atatgatttt
     6961 tgtcaatatg gaattttcta ataagcgttt tgctttgtta gaatggggtt cagcttatcg
     7021 ttggggtgaa cattatgtct taatccaagg aagcaaaggt gccatccgct tagacttatt
     7081 caactgtaaa ggaactctta agctagatgg gcaagaaagc tatttcttga ttcacgaatc
     7141 gcaagaagaa gatgatgatc ggactcgtat ctatcatagt acagagatgg atggagcaat
     7201 tgcttatggt aaaccaggta aacgtactcc attatggcta tcatctgtca ttgataaaga
     7261 aatgcgctat ctgcatgaga ttatggaagg agctccagta tcagaagaat ttgcaaaact
     7321 tttgacaggt gaagctgccc tagaagcaat tgctactgca gatgcttgta cccagtctat
     7381 gtttgaagat cgcaaagtaa gattgtcaga aattgtaaaa taaattttgg tattctccta
     7441 tctataggtc gacttgctcc tctgaaagta cttttagagg agctgtttga ctttgctagt
     7501 ttttgaaact gaaatctatt atactacaaa ctattgaaag cgttttaatt ttaaggtata
     7561 ataatctcat agaaataaag aaaaggagga aagaggatgc cacagattag caaagaagcc
     7621 ttgattgagc aaatcaaaga tggaatcatc gtttcttgtc aggctcttcc tcatgaaccg
     7681 ctttatacag aagcgggagg ggtgattccc ttgctggtca aagcggctga gcaaggtgga
     7741 gcagtcggta tccgagcaaa cagtgttcgc gatatcaagg aaattaagga agtcactaaa
     7801 cttccaatca ttgggattat caaacgtgat tatccacctc aggaaccctt catcacggct
     7861 actatgaaag aagttgatga attggcagaa ctggacatcg aggtgattgc tctggattgt
     7921 accaagcgtg aacgctacga tggtttggaa attcaagagt tcattcgtca ggttaaggag
     7981 aaatatccta atcagctttt gatggctgat actagtatct tcgaagaagg gctagcagct
     8041 gtagaagcag gaattgactt tgtcggaaca accttatcag gctacacatc ctacagtcca
     8101 aaagtagacg gtccagattt tgaattgatt aagaaactct gtgatgccgg tgtagatgtc
     8161 attgcagaag gaaaaattca tacaccagaa caagccaaac aaatccttga atatggagtg
     8221 cgaggcatcg ttgttggtgg cgccattact agaccaaaag agattacaga acgcttcgtt
     8281 gctagtctta aataagatgt gagggggagt tttatgttta aagttttaca aaaagttgga
     8341 aaagctttta tgttacctat agctatactt cctgcagcag gtctactttt ggggattggt
     8401 ggtgcacttt caaacccaac cacgatagca acttatccaa tactagacaa tagtattttt
     8461 caatcaatat tccaagtaat gagctctgca ggagaggttg tattcagtaa tttgtcacta
     8521 cttctctgtg tgggattatg tattggctta gcgaaacgag ataaaggaac cgctgcgtta
     8581 gcaggagtaa ctggttactt agttatgact gcaacgatca aagctttagt aaaacttttt
     8641 atggcagaag gatctgcaat tgatactgga gttattggag cattagttgt cggaatagtt
     8701 gccgtatatt tgcacaaccg atataacaat attcaattac cttccgcttt aggattcttt
     8761 ggaggttcac gcttcgttcc tattgttaca tcgttctctt ctatcttgat tggctttgtc
     8821 ttctttgtta tttggccacc tttccaacaa cttcttgttt ctacaggtgg atatatttct
     8881 caggcgggtc caattggaac ttttctatat ggatttttaa tgagactttc tggagcagta
     8941 ggcttacatc atataattta ccctatgttt tggtatactg aacttggtgg tgttgaaact
     9001 gttgcaggac aaacagtggt tggagctcaa aaaatatttt ttgctcaatt agccgatccg
     9061 gcccattctg gattatttac agaaggaaca aggttttttg caggtcgttt ctcaacaatg
     9121 atgttcggtt taccggctgc ctgtttagcg atgtaccata gtgttcctaa aaatcgtcgt
     9181 aaaaaatacg cgggtttgtt ttttggagtt gctttaacat cttttattac cggtattaca
     9241 gaaccaattg aatttatgtt tctattcgtc agtccggttc tatatgttgt tcacgcattc
     9301 cttgatggtg ttagcttctt tattgcagac gtcttaaata tttcaatagg aaacacattt
     9361 tcaggaggtg taatcgattt cacattattt ggaattttgc aggggaacgc taagacgaat
     9421 tgggttcttc agattccatt tggacttatt tggagtgttt tgtattatat tatttttaga
     9481 tggttcatta ctcaattcaa cgttctaacg ccagggcgag gagaagaagt agattctaaa
     9541 gaaatttctg aatccgcaga ttcaacttca aatactgcag attatttaaa acaggatagc
     9601 ctacaaatta tcagagcctt gggtggatca aataatatag aagatgtaga tgcttgtgtg
     9661 acacgtttac gtgtagctgt aaaagaagtt aatcaagttg ataaagcact tttaaaacaa
     9721 attggtgcag ttgatgtctt agaagtgaag ggtggcattc aagcaatcta tggagcaaaa
     9781 gcaatcttat ataaaaatag tattaatgaa attttaggtg tagatgatta agtacttact
     9841 gacttaataa aaaacagagg agagtgatgg atgagtagga tgaaatgaaa tcgcatacaa
     9901 gaaataaaga actcattatc caagttggat acgcttatta cataggagaa tacaaatgaa
     9961 atttagaaaa ttagcttgta cagtacttgc gggtgctgcg gttcttggtc ttgctgcttg
    10021 tggcaattct ggcggaagta aagatgctgc caaatcaggt ggtgacggtg ccaaaacaga
    10081 aatcacttgg tgggcattcc cagtatttac ccaagaaaaa actggtgacg gtgttggaac
    10141 ttatgaaaaa tcaatcatcg aagcgtttga aaaagcaaac ccagatataa aagtgaaatt
    10201 ggaaaccatc gacttcaagt caggtcctga aaaaatcaca acagccatcg aagcaggaac
    10261 agctccagac gtactctttg atgcaccagg acgtatcatc caatacggta aaaacggtaa
    10321 attggctgag ttgaatgacc tcttcacaga tgaatttgtt aaagatgtca acaatgaaaa
    10381 catcgtacaa gcaagtaaag ctggagacaa ggcttatatg tatccgatta gttctgcccc
    10441 attctacatg gcaatgaaca agaaaatgtt agaagatgct ggagtagcaa accttgtaaa
    10501 agaaggttgg acaactgatg attttgaaaa agtattgaaa gcacttaaag acaagggtta
    10561 cacaccaggt tcattgttca gttctggtca agggggagac caaggaacac gtgcctttat
    10621 ctctaacctt tatagcggtt ctgtaacaga tgaaaaagtt agcaaatata caactgatga
    10681 tcctaaattc gtcaaaggtc ttgaaaaagc aactagctgg attaaagaca atttgatcaa
    10741 taatggttca caatttgacg gtggggcaga tatccaaaac tttgccaacg gtcaaacatc
    10801 ttacacaatc ctttgggcac cagctcaaaa tggtatccaa gctaaacttt tagaagcaag
    10861 taaggtagaa gtggtagaag taccattccc atcagacgaa ggtaagccag ctcttgagta
    10921 ccttgtaaac gggtttgcag tattcaacaa taaagacgac aagaaagtcg ctgcatctaa
    10981 gaaattcatc cagtttatcg cagatgacaa agagtgggga cctaaagacg tagttcgtac
    11041 aggtgctttc ccagtccgta cttcatttgg aaaactttat gaagacaaac gcatggaaac
    11101 aatcagcggc tggactcaat actactcacc atactacaac actattgatg gatttgctga
    11161 aatgagaaca ctttggttcc caatgttgca atctgtatca aatggtgacg aaaaaccagc
    11221 agatgctttg aaagccttca ctgaaaaagc aaacgaaaca atcaaaaaag ctatgaaaca
    11281 atagtcctta gttattctat aaaaagtagt tttttaaaga acctaagagt gtataccccc
    11341 ttttccctct acacagatag tgtaagaaaa gggggctttt gtttaaaatg taagaaactg
    11401 tcacgaaatt aaaatgaagt tcttacataa gcgaatcata aaaaatttca ttttgatttt
    11461 aaaacagttc aagaaagtca aaaaattatt ctatttgaaa gagaggtgcc gactgtgaaa
    11521 gtcaataaaa tccgtatgcg ggaaacagtg atttcctacg ctttcctagc accagtatta
    11581 ttcttctttg tcatctttgt gttggctccg atggtgatgg gcttcattac aagtttcttt
    11641 aactactcaa tgactaaatt tgagtttgta ggcttggata actatatccg tatgtttaaa
    11701 gatcctgtct ttacaaaatc tctgattaac acagttattt tggttattgg atctgtacca
    11761 gttgttgttc tattctcact ctttgtagca tctcagacct atcatcaaaa tgtcattgcc
    11821 agatccttct accgtttcgt cttcttcctt cctgttgtaa cgggtagtgt tgccgtgaca
    11881 gttgtttgga aatggattta tgacccgcta tcagggattc taaactttgt ccttaagtca
    11941 agccacatca tcagccaaaa catttcttgg ttgggagata aaaactgggc attgatggcg
    12001 attatgatta ttctcttgac cacttcagtt ggtcagccca tcatccttta tatcgctgcc
    12061 atggggaata ttgacaattc actggttgaa gcggcgcgtg ttgatggtgc aactgagttt
    12121 caagtttttt ggaagattaa atggccaagc cttcttccaa caactcttta tattgcaatc
    12181 atcacaacaa ttaactcatt ccagtgtttc gccttgattc agcttttgac atctggtggt
    12241 ccaaactact caacaagtac cttgatgtac tacctttacg aaaaagcctt ccaattgaca
    12301 gaatacggct atgccaacac aattggtgtc ttcttggcag tcatgattgc tatcgtaagc
    12361 tttgttcaat ttaaagtact tggaaacgac gtagaatact aaagaaagga gacagctatg
    12421 caatctacag aaaaaaaacc attaacagcc tttactgtta tttcaacaat cattttgctc
    12481 ttgttgactg tgctgttcat ctttccattc tactggattt tgacaggggc attcaaatca
    12541 caacctgata caattgttat tcctcctcag tggttcccta aaatgccaac catggaaaac
    12601 ttccaacaac tcatggtgca gaaccctgcc ttgcaatgga tgtggaactc agtatttatc
    12661 tcattggtaa ccatgttctt agtttgtgca acctcatctc tagcaggtta tgtattggct
    12721 aaaaaacgtt tctatggtca acgcattcta tttgctatct ttatcgctgc tatggcgctt
    12781 ccaaaacaag ttgtccttgt accattggta cgtatcgtca acttcatggg aatccacgat
    12841 actctctggg cagttatctt gcctttgatt ggatggccat tcggtgtctt cctcatgaaa
    12901 caatttagtg aaaatattcc tacagagttg cttgaatcag ctaaaatcga cggttgtggt
    12961 gagattcgta ccttctggag tgtagccttc ccgattgtga aaccagggtt tgcagccctt
    13021 gcaatcttta ccttcatcaa tacttggaat gactacttca tgcaattggt aatgttgact
    13081 tcacgtaaca atttgaccat ctcacttggg gttgcgacca tgcaggctga aatggcaacc
    13141 aactatggtt tgattatggc aggagctgcc cttgctgctg ttccaatcgt cacagtcttc
    13201 ctagtcttcc aaaaatcctt cacacagggt attactatgg gagcggtcaa aggataatac
    13261 tctgcgaaaa tcgaatgcaa actacgtcag cttcaccttg ccatacttaa gtattgcctg
    13321 cggttagctt cctagtttgt tcttcaattt tcattgagta taggaaaatc aatctatcaa
    13381 gatacagaag tatattttat agatttagag aatatagagg ttataagtgt ctacaaaatg
    13441 gagggtatgc agttacttta tgaagttttg tcagacactt atcaacttaa gaatggtttt
    13501 agttaactat cagaaacgaa ggaaagagta tgatttttga cgatttgaaa aacatcacct
    13561 tttacaaagg gattcatccc aatttagaca aggctatcga ctacctctac caacatcgta
    13621 aagattcatt cgaattagga aagtatgaga ttgatgggga taaagtcttt ctagttgttc
    13681 aggaaaatgt cctcaatcaa gttgagaata atcaatttga acaccataag aactatgcag
    13741 atttgcattt gctgatagaa gggcatgaat attcgagcta cggttcacgt atcaaagacg
    13801 aggcagtagc attcgacgaa gcgagtgaca ttggctttgt tcattgtcat gaacactacc
    13861 cactcttatt gggttatcac aattttgcga ttttcttccc aggtgagcca catcagccaa
    13921 atggttatgc aggcatggaa gaaaaggttc gaaaatatct ctttaaaatt ttgattgatt
    13981 aaaaatagga tgaattgttt ttttgtaaag ctttgacaat actctaccat gaaattgatc
    14041 tttgtgaggt agagaaatga gaataaaata tttaaaaatt ggtatcttct aagtatgctg
    14101 caagagccag tttcttagat ggacagggga ttacagttga tgagatgtcc tggataatta
    14161 atggcattgt gaatggattg attggtagat acataaaatt aggtatttat gcagccaagt
    14221 atggtattag tatggtgcga tcgatcttaa gtagggcagc tgcaactgcg gcagcaagag
    14281 taggattatt gaccaagatt tttggatgga ttttacgaat agctgtgctc gcagctgatg
    14341 tatatggtaa ttttgccaac aatattgctg tagcttggga tgcacatgat aaaatcccta
    14401 acaatggtcg tataaacttt taaaatgcga gaatgaaagc actttgtatt tttttattga
    14461 atatgttagc ttggacagtg cttgcaatga cagtccgtgg agggctagat ggatttaatg
    14521 agcatacttg gagtactatt ttaattgctt cgctgtccgg gatatatata gattatatgt
    14581 ccatagataa aaatagaaaa aagtccaaaa gaaaaaatag atttgttcat ggtagggact
    14641 tatgaaagct ttactgacaa aaaagaaaac agtttacaaa gaaaaatgat ggaggagcaa
    14701 acatggcaca aaaaggagta agccttatca aggcagcatt tgatacagat aactttctca
    14761 tgcgttttag tgagaaagtc ttggacatcg tgacagccaa tcttcttttt gtcgtctctt
    14821 gtttgcccat cgtgacgatt ggagtggcta aaatcagcct ctacgagacc atgttcgaag
    14881 ttaagaagag cagacgggtg cctgttttta aaatctatct aagatctttc aagcaaaatc
    14941 tgaaactagg tcttcagctg ggtttaatgg agttaggaat tgtgtttctt accctttcag
    15001 atctctatct tttctggggt caaacagctc tgcccttcca attgctgaaa gccatttgtt
    15061 taggtattct gatttttctt actatcgtga tgctggctag ttaccctatc gcggcacgtt
    15121 atgacctatc ttggaaagaa attcttcaaa aaggattgat gttggctagt tttaactttc
    15181 cttggttctt cctcatgtta gccattcttg tcctcattgt gatggttctt tatctgtccg
    15241 ccttcagtct actcttaggt ggctcagtct tcttactttt tgggtttgga ctattggtct
    15301 ttatccagac tggattgatg gagaaaattt tcgcaaaata ccaataggag ccttatttct
    15361 gaaactactt tcaaacgctc caaacgctat tctataagcg agaaactaaa atctaagtgt
    15421 ctataagata ttgaaagcct tttcggtaaa gtgtatacta aagaagtaaa gaaaaatctg
    15481 ctttagcaga aaataaaaat aataggagaa aatctatgtc agatttgaaa aaatacgaag
    15541 gtgtcattcc agccttctac gcatgttatg atgatcaagg agaagtaagc ccagaacgta
    15601 cgcgtgcctt ggttcaatac ttcattgata aaggtgttca aggtctttat gtcaatggtt
    15661 cttctggtga atgtatctac caaagcgttg aagatcgcaa gttgattttg gaagaagtca
    15721 tggcggtagc caaaggtaaa ttgaccatta ttgctcatgt tgcctgcaac aatactaaag
    15781 acagtatgga acttgctcgc catgctgaaa gcttgggagt agatgctatt gcaacgattc
    15841 caccaattta tttccgcttg ccagaatact cagttgccaa atactggaac gatatcagtt
    15901 ctgcagctcc aaacacagac tacgtgattt acaacattcc tcaattggca ggggttgctt
    15961 tgactccaag cctttacaca gaaatgttga aaaatcctcg tgttatcggt gttaagaact
    16021 cttctatgcc agttcaagat atccaaacct ttgtcagcct tggtggggaa gaccatatcg
    16081 tctttaatgg tcctgatgag cagttcctag gaggacgcct catgggtgct agggctggta
    16141 tcggtggtac ttatggtgct atgccagaac tcttcttgaa actcaatcag ttgattgcgg
    16201 ataaggacct agaaacagcg cgtgaattgc agtatgctat caacgcaatc attggtaaac
    16261 tcacttctgc tcatggaaat atgtacggtg tcattaaaga agtcttgaaa atcaatgaag
    16321 tcttgaatat tggatctgtt cgttcaccat tgacaccagt gactgaagaa gatcgtccag
    16381 ttgtagaagc ggctgctgcc ttgattcgtg aaaccaagga gcgcttcctc taatctaaaa
    16441 ggaggtattt atgacatact acgttgcaat tgatatcggt ggaaccaaca tcaagtatgg
    16501 tttggttgat caagaggggc aacttcttga atcgcatgaa atgccaactg aggcgcataa
    16561 gggtggacct catatcttac aaaagaccaa agatatcgta gctagttatt tagaaaaagg
    16621 cccagtagca ggtgttgcca tatcttctgc tgggatggtg gatccggata agggtgagat
    16681 tttctatgct gggcctcaaa tccctaacta cgcaggcacc cagttcaaaa aggaaatcga
    16741 agaaagcttt actattcctt gtgagattga aaatgatgtc aactgtgcag gtcttgctga
    16801 ggcagtatct ggttcaggca agggagcaag tgtgacactt tgcttgacca ttggaaccgg
    16861 tatcggtggt tgcttgatta tggataggaa agtcttccat ggttttagta attcagcctg
    16921 tgaagtcggg tatatgcata tgcaggatgg agcttttcag gatctcgctt ctacaacagc
    16981 tttagtgaaa tatgtagctg aagcccatgg agaagatgtt gatcagtgga atggccgacg
    17041 tatctttaag gaagctactg aaggaaacaa aatctgcatg gaaggtattg accgtatggt
    17101 tgactatcta ggaaaaggtc tggcaaatat ttgttatgtg gccaatccag aagtggttat
    17161 tcttggtggt ggtatcatgg ggcaagaggc tatcctcaaa cctaagatcc gcacagcctt
    17221 gaaagaggca ttggtaccaa gtttagcaga aaaaacacga ttagaatttg cccatcacca
    17281 aaatacagca gggatgttgg gtgcatatta tcattttaag acaaaacaat cctagtttgg
    17341 ctcagccaaa ctaggatttt cttacacgtt tttgtctacg atagccgttg agttttttat
    17401 tttcccagta gctattaaag attttttcct tgctttcgcg attgatttcc aaaaagtagg
    17461 cataaatcaa atcgataaag aagagcatag gaagttgagc ggatattcgt tggatgtagg
    17521 agggttggct gtgggtggct acaagaacag tctctgtata ggtctggcta tctttattgg
    17581 gaacacttgt aaagagtaca gtctttgccc ccatctcctt ggcatctaat agactatcta
    17641 aaatagaagg agttgagcct gaaagtgaga aacccagtac tagacaattt tcatccatga
    17701 tgctggttgt ccaggcaaag ccgtcttggt ctgtcaaagc ttcgcagacc acacctagtc
    17761 gcataaaacg taatttcatt tcacgggcga cgaggccaga actccctgtt ccaaagaagt
    17821 agatacgctc agcatcttcg attagctggg caattcgctc tagttggatt tcgtcaatca
    17881 agtcttgtgt ttgttccctc atattgctat aacttctgag gactcgtttg gtcagtggac
    17941 tatgcttgga gacttggttg gcttgatttt ctgcctgatg ttggtattgg aaaataaatt
    18001 ctcggtagcc agtaaagcca cactttttag caaagcgggt caaagcagct tgagaaatat
    18061 gtaatttttg ggtgacttgt tgagaagata aatcatctgt aatcgtttca gcttgcaaaa
    18121 aatagcgagc gatttcttgc tctaggtctg tcatttcttc aaaatgtgaa tcaatgatag
    18181 ttgcgatatc tggtttgtcc atagggaaag ctcctttaca tgggtcatac tggaagacta
    18241 gatcagggaa tagtcacact tcattataac acataatata aggatagata aataaaaacg
    18301 catctctgtt ttaaaaacga aaaaatcgaa aaagcttctc tcttttccat aattttctac
    18361 tcaaattgtg gtacaattaa gagtaagatt ttaagttaga aatgagactg atttgtatga
    18421 gaaaatttaa cagccattcg attccgattc ggcttaattt attgttttca atcgtcattt
    18481 tactctttat gaccattatt ggtcgtttgt tgtatatgca ggttttgaac aaggattttt
    18541 acgaaaaaaa gctagcctca gctagtcaga ccaagattac aagcagttca gcccgtgggg
    18601 aaatttatga tgctagtgga aaacctttgg tagaaaatac gttaaagcag gttgtttcct
    18661 ttacgcgtag caataaaatg acggctacag acttaaaaga aacagctaaa aagttactga
    18721 cttatgtgag catcagttct ccaaatttga cagaacgcca gctggcggat tactatttgg
    18781 ctgatcctga aatctataaa aaaacagtgg aagctctccc aagtgagaaa cgcttggatt
    18841 cagatggcaa tcgtctatcc gaatcagaac tgtataacaa tgcggtcgat agtgtccaaa
    18901 caagtcaact aaactataca gaggatgaaa agaaagaaat ctatcttttt agtcagttaa
    18961 atgctgttgg aaactttgcg acaggaacca ttgctacaga tcctctaaat gattctcagg
    19021 tggctgttat tgcctctatt tcaaaggaga tgcctggcat tagtatttct acttcttggg
    19081 atagaaaggt tttggaaact tccctttctt ctatagttgg gagtgtatcc agtgaaaaag
    19141 ctggtctccc agcggaagaa gcagaagcct atcttaaaaa aggctattct ctaaatgatc
    19201 gtgtaggaac ctcctatttg gaaaagcaat atgaagagac cttacaagga aaacgctcgg
    19261 taaaagaaat ccatctggat aaatatggca atatggaaag cgtggataca attgaggaag
    19321 gtagtaaggg aaacaatatc aaactgacca ttgatttggc tttccaagat agcgtggatg
    19381 ctttactgaa aagttatttc aattccgagc tagaaaatgg tggagccaag tattctgaag
    19441 gtgtctatgc agtcgccctt aacccaaaaa caggtgcggt tttgtctatg tcagggatta
    19501 aacatgactt gaaaacggga gagttgacgc ctgattcctt gggaacggta accaatgtct
    19561 ttgttccagg ttcggttgtc aaggcggcga ccatcagctc aggttgggaa aatggagtct
    19621 tgtcaggaaa ccagaccttg acagaccagt ccattgtctt ccaaggttca gctcccatca
    19681 attcttggta tactcaggct tacggttcat tccctatcac agcggtccaa gctctggagt
    19741 attcatcaaa tacctatatg gtccaaacag ccttaggtct tatggggcaa acctatcaac
    19801 ccaatatgtt tgtcggcacc agcaatctag agtctgctat ggagaaactg cgttcaacct
    19861 ttggcgaata tggcttgggt actgcgacag gaattgacct accagatgaa tctactggat
    19921 ttgttcccaa agagtatagc tttgctaatt acattactaa tgcctttggg cagtttgata
    19981 actatacgcc gatgcagttg gctcagtatg tagcaactat tgcaaataat ggtgttcgtg
    20041 tggctcctcg tattgttgaa ggcatttatg gtaataatga taagggagga ctgggtgact
    20101 tgattcagca actgcaaccg acagagatga ataaggtcaa tatatccgac tccgatatga
    20161 gcatcttgca ccaaggtttt tatcaggttg cccatggtac tagtggattg acaactggac
    20221 gtgccttttc aaatggcgcc ttggtatcca ttagcggaaa aacaggtaca gccgaaagct
    20281 atgtggcaga tggtcagcaa gcaaccaata ccaatgcggt ggcctatgcc ccatctgata
    20341 atccccaaat cgctgttgca gtggtctttc ctcataatac caatctaaca aatggtgtag
    20401 gaccttccat tgcgcgtgac attatcaatc tgtatcaaaa ataccatcca atgaactaga
    20461 aaggaaatta tgctttatcc aacacctatt gccaagttga ttgacagtta ttctaagtta
    20521 ccaggtatcg ggattaagac ggctacgcgt ctggcctttt atacgattgg gatgtctgct
    20581 gatgatgtca atgaatttgc aaaaaatctc ctttctgcta agagagaatt gacatattgt
    20641 tctatttgtg gacgtttgac agacgacgat ccttgttcta tctgtactga tccgactcgt
    20701 gaccagacaa caattttagt tcttgaggat agtagagatg tggcagccat ggaaaatatc
    20761 caagaatacc atggactcta tcatgtcctt catggcctca tttctcctat gaatggtatc
    20821 agtccggacg atatcaatct caagagcctt atgactcgtc ttatggatag tgaagtttca
    20881 gaagtgattg tggcgactaa tgctacagcg gatggtgaag cgacttccat gtatctttca
    20941 cgtttgctca agccggctgg tatcaaggtt acgcgtctag cacgaggtct cgctgtggga
    21001 gcggacattg agtatgcgga cgaagtgaca ctcttacgag ccattgaaaa tcggacagag
    21061 ttgtaagtgt aggcaaattt acgaacttca ttcatttata aaaaatcaaa gaggctgaaa
    21121 atcgttccta tcggcctctt tttgtatagt gtgatgagta ggatcaggtt caagttttaa
    21181 aaaaccaagc aaatatgata tactaaagag cgagtattct agtagaatta ggacaaataa
    21241 tatgaaacaa acgattattc ttttatatgg tggacggagt gcggaacgcg aagtctctgt
    21301 cctttcagct gagagtgtca tgcgtgcggt caattacgac cgtttcacag tcaagacttt
    21361 ctttatcagt cagtcaggtg actttatcaa aacacaggaa tttagtcatg ctccggggca
    21421 agaagaccgt ctcatgacca atgaaaccat tgattgggat aagaaagttg caccaagtgc
    21481 tatctacgaa gaaggtgcag tggtctttcc agtccttcac gggccaatgg gagaagatgg
    21541 ctctgttcaa ggattcttgg aagttttgaa aatgccttac gttggttgca acattttgtc
    21601 atcaagtctt gccatggata aaatcacgac taagcgtgtt ctggaatctg ctggtattgc
    21661 ccaagttcct tatgtggcta tcgttgaagg cgatgatgtg actgctaaaa tcgctgaagt
    21721 ggaagaaaaa ttggcttatc cagtcttcac taagccgtca aacatggggt ctagtgtcgg
    21781 tatttctaag tctgaaaacc aagaagaact ccgtcaagcc ttaaaacttg ccttccgata
    21841 tgacagccgt gtcttggttg agcaaggagt gaatgcccgt gaaattgagg ttggcctctt
    21901 gggtaactac gatgtcaaga gcacgctacc tggagaagtt gtcaaggacg ttgcctttta
    21961 tgactacgat gccaagtata ttgataacaa gattactatg gatattcctg ccaaaatcag
    22021 tgatgatgtg gtggctgtca tgcgtcaaaa tgcagaaaca gccttccgtg ccattggtgg
    22081 ccttggtcta tctcgttgcg atttcttcta tacagataag ggagagattt ttctcaacga
    22141 gctcaatacc atgccaggtt ttacccagtg gtctatgtac ccactacttt gggacaatat
    22201 ggggatcagc tacccagaac taatcgagcg tttggttgac cttgccaagg aaagttttga
    22261 caagcgcgaa gcgcatttga tataaaatga aagagagggt agaagccaga accatcactg
    22321 cacggtgact agagttctcg gacttcagcc ctttttaaag gagtagaaat gaaattaaca
    22381 atccatgaaa ttgcccaagt tgttggagcc aaaaatgata tcagtatctt tgaggacacc
    22441 cagttagaaa aagctgagtt tgatagtcgt ttgattggaa ctggagattt atttgtgcca
    22501 cttaaaggtg cgcgtgatgg ccatgacttt attaaaacag cctttgaaaa tggtgcagca
    22561 gtaaccttgt ctgagaaaga ggtctcaaat catccttaca ttctagtaga tgatgttttg
    22621 acagcctttc aatccctagc atcctactat cttgaaaaaa cgactgttga tgtctttgct
    22681 gttacaggtt caaatggcaa gacaacgact aaggatatgt tggcgcattt actgtcaaca
    22741 agatacaaga cctacaaaac acaaggcaat tacaataatg agattggcct tccttacaca
    22801 gttctccaca tgcctgaagg aacagaaaag ttggtcttgg agatggggca ggaccacttg
    22861 ggagatattc atctcttgtc tgaattggct cgtccaaaaa cagccatcgt gaccttggtt
    22921 ggagaagctc atttggcctt tttcaaagat cgttctgaga ttgctaaagg aaaaatgcaa
    22981 attgcagacg gaatggcttc aggttccttg cttttagcgc cggctgaccc tatcgtagag
    23041 gactatttgc caactgataa aaaggtggtc cgttttgggc aaggggcaga gttggaaatc
    23101 acagacttgg ttgagcgcaa agatagtcta accttcaagg ccaatttctt agagcaagcc
    23161 cttgatttgc cagtaactgg taagtacaat gctaccaatg ctatgattgc atcctatgtt
    23221 gccctacaag aaggagtttc agaggagcaa attcatcagg ccttccaagg tcttgaattg
    23281 acgcgtaatc gtactgagtg gaagaaagca gccaatggag cagatatcct gtcagacgta
    23341 tataatgcca atccaactgc tatgaagctg attttagaga ctttctctgc cattccagcc
    23401 aatgaaggtg gcaagaaaat tgcagtgttg gcggatatga aggagcttgg tgaccagtct
    23461 attcaactcc ataaccagat gattttgagc ctttctccag atgtgcttga taccgttatt
    23521 ttctatggag aagacattgc tgaattagcc caattggcca gtcaaatgtt cccaataggc
    23581 cacgtttact acttcaagaa aacagaagac caagaccaat ttgaagacct agccaagcag
    23641 gttaaggaaa gccttggagc ctatgaccaa atcttgctaa aaggttctaa ctctatgaac
    23701 ctagctatgt tggtagaaag tttagaaaat gaaaccaagt gattttgtta agtatctgca
    23761 aagaatgatt gcccttacag atactggctt aacctttaca aaagatcctt ttgaccgtga
    23821 gcgctacgag gacttgcgaa gtctgttatc tgaaatgttg aatcagggat cagacctgga
    23881 tgcagaagaa gtagcagaag tcttgaaacc aacttcagct tatgcgactc ccttaatgga
    23941 cgtccgtgct tggattgttg aggatgagaa gatttgtctg gttaggggac aaggagagga
    24001 tagttgggct ttgccaggtg gctttggtga agtcggctat tctccaactg aaaatattct
    24061 caaggaaatt gaagaagaaa ccggttttaa agccaaagtt gaaagactgc tagctgtttt
    24121 tgataccaat cgtttccaac tacagagcaa acaatatgca aagtttgttt ttgaatgtaa
    24181 gcttctcgat ggacaattcc aagaaaatca agaaatcgct gaccttcaat tttttgcaat
    24241 tgaccaactg ccgaatttat ctgaaaaacg cattaccaag gagcaaatcg agattctttg
    24301 gcaggtttat caaggtcaga gggagcaata tcttgactaa gaagatgatt atcgtatttc
    24361 taaatccatt tttgacaact agcatggtat aataatatgc aggaaaattt tgaattatga
    24421 ggaagactag atgaatttat gggatatttt ctttacgact caggcaaccg agccgcccaa
    24481 atttgacctt ttttggtatg ttagcctatt tacgctctta gccttaacct tttatacagc
    24541 ccatcgctat cgtgaaaaga aggtttacca acgatttttc caaatcttgc agactgttca
    24601 gttaatcctt ctttatggtt ggtactgggt caatcatatg ccactgtcag aaagcctacc
    24661 cttttaccat tgccgtatgg ctatgtttgt ggtactcttg cttcctggtc agtccaaata
    24721 taaacaatac tttgcattat tgggaacatt tgggacatta gcagcctttg tttatccagt
    24781 gccagatgct tacccttttc cacatatcac cattctatcc tttatctttg gtcatttagc
    24841 actcttgggg aactctctag tttatctatt gagacagtat aatgcgcgat tgctggatgt
    24901 gaagggaatt tttctcatga cctttgccct aaatgccttg atttttgtgg tcaatttggt
    24961 gacaggtggc gattacggat ttttgacaaa accgccattg gttggggatc acggtctagt
    25021 agctaattat ttacttgttt caattgtgct ggtagctact atcagtttga ctaagaaaat
    25081 cttagaattc tttttagctc aagaagcaga aaaaatgatt gcaaaggaag cttaacacag
    25141 agctttcttt tttgctctta gagagttttt acaagcagct tataaaataa gaatttttga
    25201 atagacaaac tcaaaaaatg gctgggaaat ttaggaaaaa agcaagcacg attaaatttt
    25261 ttgtgttata atattttgtg aatagctatg cctatgttta gctatggaat aatacgaagt
    25321 gcgaaacttg gaagatagag aggaagcgat gtaatggcta gagaaggctt ttttacaggt
    25381 ctagatattg gaacaagctc tgtcaaggtg cttgtggccg agcagagaaa tggtgaatta
    25441 aatgtaattg gcgtgagtaa tgccaaaagt aaaggtgtaa aggatggaat tattgttgat
    25501 attgatacag cagcaactgc tatcaagtca gccatttccc aagcggaaga aaaggcaggc
    25561 atttcgatta aatcagtgaa tgtcggcttg cctggtaatc ttttgcaggt agaaccaact
    25621 caggggatga ttccagtaac atctgatact aaggaaatta cggatcaaga tgttgaaaat
    25681 gttgtcaaat cagctttgac aaagagtatg acacctgacc gtgaagtcat tacctttatt
    25741 cctgaagaat ttattgtgga tggtttccaa gggattcgtg acccacgtgg tatgatgggg
    25801 gttcgccttg aaatgcgtgg tttgctttat acaggacctc gtactatctt gcacaatttg
    25861 cgtaagacgg ttgagcgtgc aggtgttcag gttgaaaatg ttatcatttc accactagca
    25921 atggttcagt ctgttttgaa tgaaggggaa cgtgaatttg gtgctacagt gattgatatg
    25981 ggggcaggtc aaacgactgt cgctacaatc cgtaatcaag aactccagtt cacacatatt
    26041 ctccaagaag gtggagatta tgtaactaaa gatatctcca aggttttgaa aacctctcgc
    26101 aaattagcgg aaggcttgaa actgaattac ggggaagcct atccgcctct tgcaagcaaa
    26161 gaaaccttcc aagtagaggt tattggagaa gtggaagcag tcgaagtgac ggaagcctac
    26221 ttgtcagaaa ttatttctgc acgaatcaag cacatccttg aacaaatcaa gcaagaatta
    26281 gatagaaggc gtctattgga cctccctggt ggtattgtct taatcggtgg gaatgccatt
    26341 ttaccaggta tggttgagct tgctcaggaa gtctttggcg tccgtgtcaa gctttatgtt
    26401 ccaaatcaag ttggtatccg taatccagcc tttgcgcatg tgattagttt atcagaattt
    26461 gcgggtcaat taacagaagt taatcttttg gctcagggag cgataaaagg tgagaatgac
    26521 ttaagtcatc agccaattag ttttggtggg atgctgcaaa aaacagctca gtttgtacaa
    26581 tcaacgcctg ttcaaccagc tcctgctcca gaagtagagc cggtggcgcc tacagaacca
    26641 atggcggatt tccaacaagc ttcacaaaat aaaccgaaat tagcagatcg tttccgtggc
    26701 ttgatcggaa gcatgtttga cgaataaaga ggaaaaataa attatgacat tttcatttga
    26761 tacagctgct gctcaagggg cagtgattaa agtaattggt gtcggtggag gtggtggcaa
    26821 tgccatcaac cgtatggtcg acgaaggtgt tacaggcgta gaatttatcg cagcaaacac
    26881 agatgtacaa gcattgagta gtacaaaagc tgagactgtt attcagttgg gacctaaatt
    26941 gactcgtggt ttgggtgcag gaggtcaacc tgaggttggt cgtaaagccg ctgaagaaag
    27001 cgaagaaaca ctgacggaag ctattagtgg tgccgatatg gtcttcatca ctgctggtat
    27061 gggaggaggc tctggaactg gagctgctcc tgttattgct cgtatcgcca aagatttagg
    27121 tgcgcttaca gttggtgttg taacacgtcc ctttggtttt gaaggaagta agcgtggaca
    27181 atttgctgta gaaggaatca atcaacttcg tgagcatgta gacactctat tgattatctc
    27241 aaacaacaat ttgcttgaaa ttgttgataa gaaaacaccg cttttggagg ctcttagcga
    27301 agcggataac gttcttcgtc aaggtgttca agggattacc gatttgatta ccaatccagg
    27361 attgattaac cttgactttg ccgatgtgaa aacggtaatg gcaaacaaag ggaatgctct
    27421 tatgggtatt ggtatcggta gtggagaaga acgtgtggta gaagcggcac gtaaggcaat
    27481 ctattcacca cttcttgaaa caactattga cggtgctgag gatgttatcg tcaacgttac
    27541 tggtggtctt gacttaacct tgattgaggc agaagaggct tcacaaattg tgaaccaggc
    27601 agcaggtcaa ggagtgaaca tctggctcgg tacttcaatt gatgaaagta tgcgtgatga
    27661 aattcgtgta acagttgtcg caacgggtgt tcgtcaagac cgcgtagaaa aggttgtggc
    27721 tccacaagct agatctgcta ctaactaccg tgagacagtg aaaccagctc attcacatgg
    27781 ctttgatcgt cattttgata tggcagaaac agctgaattg ccaaaacaaa atccacgtcg
    27841 tttggaacca actcaggcat ctgcttttgg tgattgggat cttcgccgtg aatcgattgt
    27901 tcgtacaaca gattcagtcg tttctccagt cgagcgcttt gaagccccaa tttcacaaga
    27961 tgaagatgaa ttggatacac ctccattttt caaaaatcgt taagtaaatg aatgtaaaag
    28021 aaaatacaga acttgttttt cgagaagttg cagaggctag tctgagtgct aatcgagaga
    28081 gtggttcggt ctctgtcatt gcagttacca agtatgtaga tgtaccgaca gcggaagcct
    28141 tgcttccgct aggtgttcat catatcggtg aaaatcgtgt agataagttt ctggaaaaat
    28201 atgaagcttt aaaagatcga gatgtgactt ggcatttgat tggtaccttg caaagacgta
    28261 aggtgaaaga tgtcattcaa tacgttgatt atttccatgc attggactca gtaaagctag
    28321 caggggaaat tcaaaaaaga agtgaccgag tcatcaagtg tttccttcaa gtaaatattt
    28381 ctaaagaaga aagcaaacac ggtttttcga gagaggaact gctggaaatc ttgccagagt
    28441 tagccggact agataagatt gaatatgttg gtttaatgac gatggcacct tttgaggcta
    28501 gcagtgagca gttgaaagag attttcaagg cggcccaaga tttacaaaga gaaattcaag
    28561 agaaacaaat tccaaatata cctatgaccg agttaagtat gggaatgagt cgtgattata
    28621 aagaagcgat tcaattcggt tccacttttg ttcgtatagg tacatcattt tttaagtagg
    28681 agagaaccat gtctttaaaa gatagattcg atagatttat agattatttt acggaggatg
    28741 aggattcaag tctcccttat gaaaaaagag atgagcctgt gtttacttca gtaaattctt
    28801 cacaggaacc ggctctccca atgaatcaac cttcacagtc ggctggtaca aaagagaaca
    28861 atatcaccag acttcatgca agacaacagg aattggcaaa tcagagtcag cgtgcaacgg
    28921 ataaggtcat tatagatgtt cgttatccta gaaaatatga ggatgcaaca gaaattgttg
    28981 atttattggc aggaaacgaa agtatcttga ttgattttca gtatatgaca gaggtgcagg
    29041 ctcgtcgttg tttggactat ttggatggag cttgtcatgt tttagctgga aatttgaaaa
    29101 aggtagcttc taccatgtat ttgttgacac cagtgaacgt tattgtaaat gttgaagata
    29161 tccgtttacc agatgaagat caacagggtg agttcggttt tgatatgaag cgaaatagag
    29221 tacgataatg atttttttaa ttcgtatgat ttataatgca gtggatattt actccctgat
    29281 tttgatagcc ttcgctgtca tgtcttggtt tccaggtgcc tacgaatcca gtttaggtcg
    29341 ttggattgta gcgttggtga aaccagtgct tgctcccttg caacgcctgc ctttacagat
    29401 agcgggtctt gatttatctg tttgggttgc gattgttttg gttcgatttt taggagaaaa
    29461 cttagtgcgt tttctggcga tgataggatg aataaaggga tttatcagca tttctccata
    29521 gaagatcgtc catttcttga caagggaatg gaatggataa agaaggtaga agatagctat
    29581 gctccttttt taactccttt tatcaatcct catcaggaga agctattaaa gattttggca
    29641 aaaacctatg gtcttgcttg tagcagtagt ggggaattcg tctcgagtga gtatgttcga
    29701 gttttattat acccagatta tttccaacca gagttttcag attttgaaat atctcttcag
    29761 gaaattgtgt attccaataa atttgaacat ttaacgcatg ctaagatttt agggacagtc
    29821 atcaatcaat tagggattga acggaaactt tttggagata tcctagtaga tgaagaacgg
    29881 gcgcagatta tgattaatca gcagtttctt cttctctttc aagatggact aaagaaaatt
    29941 ggtcgtatac ctgtttcgct ggaggaacgt cctttcaccg agaaaataga taagctagaa
    30001 cagtatcgag aactggattt atctgtgtct agttttcgat tagatgttct tttatcaaat
    30061 gttttgaaac tatctaggaa tcaagcaaac cagttgattg aaaagaaact tgtccaagta
    30121 aattatcatg tggtagacaa atcagattac actgttcaag ttggagactt gattagtgtg
    30181 agaaaatttg gtcgcttgag attacttcaa gataagggac aaacgaaaaa agagaagaaa
    30241 aaaataaccg tccagttatt attaagtaag tgaggaatag aatgccaatt acatcattag
    30301 aaataaagga caagactttt ggaactcgat tcagaggttt tgatccagaa gaagtcgatg
    30361 aatttttaga tattgtggtt cgtgattacg aagatcttgt gcgtgcgaat catgataaaa
    30421 atttgcgtat taagagttta gaagagcgtt tgtcttactt tgatgaaata aaagattcat
    30481 tgagccagtc tgtattgatt gctcaggata cagctgagag agtgaaacag gcggcgcatg
    30541 aacgttcaaa caatatcatt catcaagcag agcaagatgc gcaacgcttg ttggaagaag
    30601 ctaaatataa ggcaaacgag attcttcgtc aagcaactga taatgctaag aaagtcgctg
    30661 ttgaaacaga agaattgaag aacaagagcc gtgtcttcca ccaacgtctc aaatctacaa
    30721 ttgagagtca gttggctatt gttgaatctt cagattggga agatattctc cgtccaacag
    30781 ctacttatct tcaaaccagt gatgaagcct ttaaagaagt ggttagcgaa gtacttggag
    30841 aaccgattcc agctccaatt gaagaagaac caattgatat gacacgtcag ttctctcaag
    30901 cagaaatggc agaattacaa gctcgtattg aggtagccga taaagaattg tctgaatttg
    30961 aagctcagat taaacaggaa gtggaagctc caactcctgt agtgagtcct caagttgaag
    31021 aagagcctct gctcatccag ttggcccaat gtatgaagaa ccagaagtag ctccaatgca
    31081 tccgacaggt ccaacaccag ctacagaaac tgttgattca ataccgggat ttgaagcacc
    31141 gcaagaatct gttacaattt tataagaaat attctgagaa caatatctta tccttatatt
    31201 tccagcgagc aggagatggt gtgagtcctg cattccctat cgataagatt atcctctcaa
    31261 aaactcaagt ctgaagctag taagatttga cgtttcccac gttacgggat aagagggaga
    31321 aagactaaat ctttttccga ataaaggtgg taccacgatt ttcgtccttt ttggaagtcg
    31381 tggtttttaa tttgttatta tttataaagg agataccatg aaactcaaag acacccttaa
    31441 tcttgggaaa actgaattcc caatgcgtgc aggccttcct accaaagagc cagtttggca
    31501 aaaggaatgg gaagatgcaa aactttatca acgtcgtcaa gaattgaacc aaggaaaacc
    31561 tcatttcacc ttgcatgatg gccctccata cgctaacgga aatatccacg ttggacatgc
    31621 tatgaacaag atttcaaaag atatcattgt tcgttctaag tctatgtcag gattttacgc
    31681 accatttatt cctggttggg atactcatgg tctgccaatc gagcaagtat tgtcaaaaca
    31741 aggtgtcaaa cgtaaagaaa tggacttggt tgagtacttg aaactttgcc gtgagtacgc
    31801 tctttctcaa gtagataaac aacgtgaaga ttttaaacgt ttgggtgttt ctggtgactg
    31861 ggaaaatcca tatgtgacct tgactcctga ctatgaagca gctcaaattc gtgtatttgg
    31921 tgagatggct aataagggtt atatctaccg tggtgccaag ccagtttact ggtcatggtc
    31981 atctgagtca gcccttgctg aagcagagat tgaataccat gacttggttt caacttccct
    32041 ttactatgcc aacaaggtaa aagatggcaa aggagttcta gatacagata cttatatcgt
    32101 tgtctggaca acgactccat ttaccatcac agcttctcgt ggtttgacgg ttggtgcaga
    32161 tattgattac gttttggttc aacctgctgg tgaagctcgt aagtttgtcg ttgctgctga
    32221 attattgact agcttgtctg agaaatttgg ctgggctgat gttcaagttt tggaaactta
    32281 ccgtggccaa gaactcaacc acatcgtaac agaacaccca tgggatacag ctgtagaaga
    32341 gttggtaatt cttggtgacc acgttacgac tgactctggt acaggtattg tccatacagc
    32401 ccctggtttt ggtgaggacg attacaatgt tggtattgct aataatcttg aagtcgcagt
    32461 gactgttgat gaacgtggta tcatgatgaa gaatgctggt cctgaatttg aaggtcaatt
    32521 ctatgaaaag gtagttccaa ctgttattga aaaacttggt aacctccttc ttgcccaaga
    32581 agaaatctct cactcatatc catttgactg gcgtactaag aaaccaatca tctggcgtgc
    32641 agttccacaa tggtttgcct cagtttctaa attccgtcaa gaaatcttgg acgaaattga
    32701 aaaagtgaaa ttccactcag aatggggtaa agtccgtctt tacaatatga tccgtgaccg
    32761 tggtgactgg gttatctctc gtcaacgtgc ttggggtgtt ccacttccta tcttctacgc
    32821 tgaagatggt acagctatca tggtagctga aactattgaa cacgtagctc aactttttga
    32881 agaacatggt tcaagcattt ggtgggaacg tgatgccaaa gacctcttgc cagaaggatt
    32941 tactcatcca ggttcaccaa acggcgagtt caaaaaagaa actgatatca tggacgtttg
    33001 gtttgactca ggttcatcat ggaatggagt ggtggtaaac cgtcctgaat tgacttaccc
    33061 agccgacctt tacctagaag gttctgacca ataccgtggt tggtttaact catcacttat
    33121 cacatctgtt gccaaccatg gcgtagcacc ttacaaacaa atcttgtcac aaggttttgc
    33181 ccttgatggt aaaggtgaga agatgtctaa atctcttgga aataccattg ctccaagcga
    33241 tgttgaaaaa caattcggtg ctgaaatctt gcgtctctgg gtaacaagtg ttgactcaag
    33301 caatgacgtg cgtatctcta tggatatttt gagccaagtt tctgaaactt accgtaagat
    33361 tcgtaacact cttcgtttct tgattgccaa tacatctgac tttaacccag ctcaagatac
    33421 agtcgcttac gatgagcttc gttcagttga taagtacatg acgattcgct ttaaccagct
    33481 tgtcaagacc attcgtgatg cctatgcaga ctttgaattc ttgacgatct acaaggcctt
    33541 ggtgaacttt atcaacgttg acttgtcagc cttctacctt gattttgcca aagatgttgt
    33601 ttacattgaa ggtgccaaat cactggaacg ccgtcaaatg cagactgtct tctatgacat
    33661 tcttgtcaaa atcaccaaac tcttgacacc aatccttcct cacactgcgg aagaaatttg
    33721 gtcatatctt gagtttgaaa cagaagactt cgtccaattg tcagaattac cagaggctca
    33781 aacttttgct aaccaagaag aaatcttgga tacatgggca gccttcatgg acttccgtgg
    33841 acaagcacaa aaagccttgg aagaagctcg taatgcaaaa gttatcggta aatcacttga
    33901 agcacacttg acagtttatc caaatgaagt ggtgaaaact ctactcgaag cagtaaacag
    33961 caatgtagct caacttttga tcgtgtcaga cttgaccatc gcagaaggac cagctccgga
    34021 agctgccctt agcttcgaag atgtagcctt cacagttgaa cgtgctgctg gtgaagtatg
    34081 tgaccgttgc cgtcgtatcg acccaacaac agcagaacgc agctaccagg cagtcatctg
    34141 tgatcattgt gcaagcatcg tagaagaaaa ctttgcggac gctgtcgcag aaggatttga
    34201 agagaaataa gattgaaaag tctaggcaaa attcaatttg agaagaaaag acaactaatt
    34261 ttatagtcta ttaaacgcat tgtatcacgt ttttgaacac ctgatatgat gcgtttttta
    34321 tttattttaa aaatttgcga ggtatgactt tttatactca acaagaatca aagagaaact
    34381 tagcaagcta acagtagtaa gataaaatag gaatttgata ttagg
//
LOCUS       DBERMR010000019        33969 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.019, whole genome shotgun sequence.
ACCESSION   DBERMR010000019 DBERMR010000000
VERSION     DBERMR010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 33969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33969
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.019"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(110..346)
                     /locus_tag="ACL8A1_000981"
     CDS             complement(110..346)
                     /locus_tag="ACL8A1_000981"
                     /EC_number="3.4.26.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HGS1201770.1"
                     /translation="MDVILSAIIFGISHLILSHRDPISLLYYSLIGFFFALVYRFTDN
                     LRLTILCHSFFNFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(359..832)
                     /locus_tag="ACL8A1_000982"
     CDS             complement(359..832)
                     /locus_tag="ACL8A1_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201771.1"
                     /translation="MRNTKKLRQFGIFLLIILLSTYLPQTIGLYVTIILGLGADVYSL
                     ILTMVLVGSFLLLIWRLKKKKMLFIFEKKSWNWSFVFSLFATYVVYQILGNFWARYAH
                     LINHRNIHDEYFTVVLSNGQPTFLSTILSFVLPVIIGPVFEETLDRGYFMNTFFP"
     gene            complement(850..1035)
                     /gene="briC"
                     /locus_tag="ACL8A1_000983"
     CDS             complement(850..1035)
                     /gene="briC"
                     /locus_tag="ACL8A1_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HGS1201772.1"
                     /translation="MTGTNTFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRAG"
     gene            complement(1298..2065)
                     /locus_tag="ACL8A1_000984"
     CDS             complement(1298..2065)
                     /locus_tag="ACL8A1_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HGS1201773.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDKLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEIGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(2062..2928)
                     /gene="accD"
                     /locus_tag="ACL8A1_000985"
     CDS             complement(2062..2928)
                     /gene="accD"
                     /locus_tag="ACL8A1_000985"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HGS1201774.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNVGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(2965..4332)
                     /gene="accC"
                     /locus_tag="ACL8A1_000986"
     CDS             complement(2965..4332)
                     /gene="accC"
                     /locus_tag="ACL8A1_000986"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HGS1201775.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNANFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(4344..4766)
                     /gene="fabZ"
                     /locus_tag="ACL8A1_000987"
     CDS             complement(4344..4766)
                     /gene="fabZ"
                     /locus_tag="ACL8A1_000987"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HGS1201776.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGILTFAIGN"
     gene            complement(4763..5248)
                     /gene="accB"
                     /locus_tag="ACL8A1_000988"
     CDS             complement(4763..5248)
                     /gene="accB"
                     /locus_tag="ACL8A1_000988"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HGS1201777.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(5251..6486)
                     /gene="fabF"
                     /locus_tag="ACL8A1_000989"
     CDS             complement(5251..6486)
                     /gene="fabF"
                     /locus_tag="ACL8A1_000989"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HGS1201778.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(6508..7239)
                     /gene="fabG"
                     /locus_tag="ACL8A1_000990"
     CDS             complement(6508..7239)
                     /gene="fabG"
                     /locus_tag="ACL8A1_000990"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HGS1201779.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVVAIDGGLSM"
     gene            complement(7273..8193)
                     /gene="fabD"
                     /locus_tag="ACL8A1_000991"
     CDS             complement(7273..8193)
                     /gene="fabD"
                     /locus_tag="ACL8A1_000991"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HGS1201780.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDRTAHLAHVEDQASLVALLEK"
     gene            complement(8186..9160)
                     /gene="fabK"
                     /locus_tag="ACL8A1_000992"
     CDS             complement(8186..9160)
                     /gene="fabK"
                     /locus_tag="ACL8A1_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HGS1201781.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     VSIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVE
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWAGVVRND"
     gene            complement(9279..9503)
                     /locus_tag="ACL8A1_000993"
     CDS             complement(9279..9503)
                     /locus_tag="ACL8A1_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGS1201782.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(9563..10537)
                     /locus_tag="ACL8A1_000994"
     CDS             complement(9563..10537)
                     /locus_tag="ACL8A1_000994"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HGS1201783.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(10537..10971)
                     /locus_tag="ACL8A1_000995"
     CDS             complement(10537..10971)
                     /locus_tag="ACL8A1_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGS1201784.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(11065..11850)
                     /locus_tag="ACL8A1_000996"
     CDS             complement(11065..11850)
                     /locus_tag="ACL8A1_000996"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HGS1201785.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            12175..13539
                     /locus_tag="ACL8A1_000997"
     CDS             12175..13539
                     /locus_tag="ACL8A1_000997"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HGS1201786.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDVTGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            13542..13913
                     /locus_tag="ACL8A1_000998"
     CDS             13542..13913
                     /locus_tag="ACL8A1_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HGS1201787.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            14127..15401
                     /gene="serS"
                     /locus_tag="ACL8A1_000999"
     CDS             14127..15401
                     /gene="serS"
                     /locus_tag="ACL8A1_000999"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HGS1201788.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILIK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEIKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSATKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(15426..16325)
                     /locus_tag="ACL8A1_001000"
     CDS             complement(15426..16325)
                     /locus_tag="ACL8A1_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HGS1201789.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWGLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(16435..16983)
                     /locus_tag="ACL8A1_001001"
     CDS             complement(16435..16983)
                     /locus_tag="ACL8A1_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HGS1201790.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(17201..18523)
                     /locus_tag="ACL8A1_001002"
     CDS             complement(17201..18523)
                     /locus_tag="ACL8A1_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HGS1201791.1"
                     /translation="MLELLKSIDDFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMAIIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGVRFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(18614..18702)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(18866..21202)
                     /locus_tag="ACL8A1_001003"
     CDS             complement(18866..21202)
                     /locus_tag="ACL8A1_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HGS1201792.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRKVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDAFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(21306..21854)
                     /locus_tag="ACL8A1_001004"
     CDS             complement(21306..21854)
                     /locus_tag="ACL8A1_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HGS1201793.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQIYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(21851..22153)
                     /gene="zapA"
                     /locus_tag="ACL8A1_001005"
     CDS             complement(21851..22153)
                     /gene="zapA"
                     /locus_tag="ACL8A1_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGS1201794.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            22240..23121
                     /gene="rnhC"
                     /locus_tag="ACL8A1_001006"
     CDS             22240..23121
                     /gene="rnhC"
                     /locus_tag="ACL8A1_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HGS1201795.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TPDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLSVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLNFC
                     AKLHFKNTEKAKKRLER"
     gene            23125..23739
                     /gene="lepB"
                     /locus_tag="ACL8A1_001007"
     CDS             23125..23739
                     /gene="lepB"
                     /locus_tag="ACL8A1_001007"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HGS1201796.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            23973..26339
                     /locus_tag="ACL8A1_001008"
     CDS             23973..26339
                     /locus_tag="ACL8A1_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HGS1201797.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGEQKSHDLETIQKHITTIEEELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDLRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDECSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSKESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKRDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(26387..27670)
                     /gene="tig"
                     /locus_tag="ACL8A1_001009"
     CDS             complement(26387..27670)
                     /gene="tig"
                     /locus_tag="ACL8A1_001009"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HGS1201798.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
     gene            complement(28424..29560)
                     /locus_tag="ACL8A1_001010"
     CDS             complement(28424..29560)
                     /locus_tag="ACL8A1_001010"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HGS1201799.1"
                     /translation="MKHSVHFGAGNIGRGFIGEILFKNGFHIDFVDVNNQIIHALNEK
                     GKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQKTDIITTAIGPNILPFIAELL
                     AKGIEARRVAGNTQVLDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAV
                     DRIVPAQSHEDSLFVVVEPFNEWVVETKRLKNPDLRLKDVHYEEDLEPFIERKLFSVN
                     SGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLIAKWNFDKKELENYHK
                     VIIERFENPFIVDEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYAFD
                     YRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDQELIDQIVEYI"
     gene            complement(29622..30059)
                     /locus_tag="ACL8A1_001011"
     CDS             complement(29622..30059)
                     /locus_tag="ACL8A1_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGS1201800.1"
                     /translation="MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIE
                     RDKELSVYMGNFIAIPHGTDAAKNDVLKSGITVVQVPRGVDFGNVSNPQVATVLFGIA
                     GIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE"
     gene            complement(30061..32016)
                     /locus_tag="ACL8A1_001012"
     CDS             complement(30061..32016)
                     /locus_tag="ACL8A1_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGS1201801.1"
                     /translation="MLLTKREEQLLKAFLHVGKLSMQDMTEILQVSSRTIYRTLSDLT
                     DSMEQYGIEITKHGKYYILTGELDDLPTELEVLVEYSPQERQELITYRLLTESGFVTN
                     EALQECTKVSNVTIIQDISDIDKRLLDFDLKIERQKGYRISGDSVGKRRFLAILLTNC
                     ISVADFSTGNFGSFDILEADRTRLASQIVNKQLSGFPDMDARMKMFFAILLSLIGQEQ
                     NIENSPNTSKQALEISQKIFQAYSKQTAQFYSIQEIIYFASILDELIIKRQDNPLFTE
                     KFDGEFFYNISNLIDTVSMYTKIDFFKDKVLFNFLFHHIRLSLGVPILFQGENLPESV
                     QILVERNKFLYTVISLLVNDIFPKYLHTEYEYGMIALHFISSLGRSPEIYPVRVLLLT
                     DERRVTRDLLVSKIKSVAPFVELIDIQSLVDYHSIDLSQYDYILSTKPLTNQEIDVIS
                     SFPTVKELFELQERLQYVQAHRTIVARDAIAPEKSYDLQDYLISSSQLLSQFELVQLE
                     NNQSFEHTVEQIIQYQKNVSDRDYLTRKLLSHFQNSPMAIPNTGLVLLHSQSSKVTTN
                     SFTMFELKLPISALSMKREEEEVKRCLLMLMSKEASEEARDLMTAISQSIIENHLYTE
                     IYKTGNQSIIYQMLNTIFNEKIKKLEN"
     gene            complement(32040..33809)
                     /locus_tag="ACL8A1_001013"
     CDS             complement(32040..33809)
                     /locus_tag="ACL8A1_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HGS1201802.1"
                     /translation="MEEKVSLKVRVQKLGTSLSNMVMPNIGAFIAWGVLTALFIADGY
                     LPNEQLATVVGPMLTYLLPILIGYTGGYMIHGQRGAVVGAIATVGAITGSSVPMFIGA
                     MVMGPLGGWTIKKFDEKFQEKIRPGFEMLVNNFSAGLVGFALLLLAFYAIGPVVSTLT
                     GAVGNGVEAIVNARLLPMANIIIEPAKVLFLNNALNHGIFTPLGVEQVAQAGKSILFL
                     LEANPGPGLGILLAYAVFGKGSAKSSSWGAMVIHFFGGIHEIYFPYVMMKPTLFLAAM
                     AGGISGTFTFQLLDAGLKSPASPGSIIAIMATAPKGVWPHINVLLGVLVAAVVSFLVA
                     ALILHADKSTEDSLEAAQAATQAAKAQSKGQLVSTSVDTVVLTDSVEKIIFACDAGMG
                     SSAMGASILRDKVKKAGLEIPVSNQAISNLLDTPKTLIVTQEELTPRAKDKSPSAIHV
                     SVDNFLASPRYDEIVASLTGASPIAEIEGDIPTSAPVDSQEIDLNHIDAVVVAYGKAQ
                     GTATMGCETIRAIFRNKNIRIPVSTAKISELGEFNSKNIMIVTTISLQAEVQQAAPNS
                     QFLIVDSLVTTPEYDKMAARMYK"
BASE COUNT        10119 a         7627 c         6394 g         9829 t
ORIGIN      
        1 taggctccat aatatctata ggggatttac ccactacaaa tattatagag ccaacaataa
       61 aaagaaaaag tgtttgatag atatcaaaca cttttttctt tgcctcccac tatctaaaaa
      121 aatgataata gatataattg taaacaaaaa tccagatagg ttttgcatga ttgagaaagt
      181 taaaaaaact atggcagaga atcgttaatc tcagattgtc ggtaaaacga taaacaaggg
      241 caaaaaagaa accaatcaga ctataatata ataaactaat tggatctctg tgagatagta
      301 tcaaatggct aatcccaaag atgatagcag ataggataac atccaaataa tacttggact
      361 agggaaagaa ggtattcata aaataccctc tatcaagagt ctcctcaaaa acaggaccga
      421 tgattacagg caggacaaaa gataagatag tcgataaaaa ggttggttgt ccatttgaaa
      481 gaaccacggt aaaatactca tcatgaatat tcctatgatt aatcaaatga gcatagcgtg
      541 cccaaaaatt accgagaatc tgataaacca cataagttgc aaataaggag aagacaaatg
      601 accagttcca gctctttttt tcaaagataa agagcatctt tttctttttt aacctccaaa
      661 ttaatagaag gaaacttccc actaatacca ttgttaaaat aagagaatag acatcagctc
      721 ctaaccctaa aatgatcgtc acatacaatc caattgtttg tggtaaatag gtagatagta
      781 aaataataag caaaaatatt ccaaattgtc ttagtttctt tgtgtttctc atcgtacttt
      841 tttgaaagat taccctgctc ggaagccgta cttccaagca tctatataag aattaagtgc
      901 cccttgcctc atatagggag caaattctct ataatataac catctactat atccatcttc
      961 ccaaacagca agaccacctg aagtttgctc caagtcctca gttgaaagaa ctgtaaatgt
     1021 atttgtacct gtcattgcaa gtaccttctt aaaatatagt gatttgaaat aagatgtgaa
     1081 caactctatc aggaaagtca aattaattta tagaaatatt ttagcagcca aggtgtactg
     1141 ttatagattc aatacactat aaatttttgt aagcttacat ctacagtata catctttttt
     1201 gtctatttta tagcccatct cctcaactgt caatttttca acctgaattg catttttcca
     1261 taaaaaatga gacctttcta gtctcattta gtcattctta gtattttcta aatcgttgat
     1321 agcgttcttc cagcaactct tctagcggtt tttgtgaaag tctagccagc tccgtttgga
     1381 gttctttttt gacactctta atcagttctt tactagaaag tcctatttca gaaatcacct
     1441 tatccaccac gtccatttct aacagttcat gcgaagtgat tttcatcagt tctgctgctt
     1501 ccatagcgcg agtaccgtcc ttccataaaa tggaagcaaa gccttctgga ctgagaatgg
     1561 catagataga attttccagc atccagacac ggtccgcgac agctagagcc agagccccgc
     1621 ctgaaccacc ttcaccgata ataatggcga taataggaac tttcaggtca ctcatttcca
     1681 tgagattgcg agcgatagct tccccttgac cacgttcttc cgctccgaca ccaggataag
     1741 cacctgctgt attgataaag gtcacaactg gacggccaaa tttctcagcc tgtttcatca
     1801 accgcagtgc ctttcggtag ccttctggat gtggttggcc aaaattccgt ttgagtttgt
     1861 cttgcaaact cttgcctttt tggataccaa ccactgttac agcttgatct ccaagccaac
     1921 caataccacc aacaactgca ccatcatcac gaaaagaacg gtcaccatgt aattggataa
     1981 attcatcaaa aatgcctgtc gcaaagtcca aggttgtcaa gcgactctgc tcacgcgctt
     2041 ctctgactat ttttgcaata ttcatctagg actccctcca tgcaatctga ctaggctagc
     2101 aatcgtatct ggtaagtctc ttcttttgac aatagcatcc acaaagccat gttctaatag
     2161 gaattctgcc ttttggaaat cctcaggcaa gctttcacga accgtatttt caatcacacg
     2221 acgcccagca aaaccaacca agctctgtgg ttcagccaga atgatatcgc cttccatagc
     2281 gaaagaagct gtcacaccac cagtcgttgg atctgtcaaa atggtcaggt aaaagagacc
     2341 aacatttgaa tggcgtttaa ccgccgcaga gatcttagcc atctgcatga gactcatgat
     2401 tccttcctgc atacgggctc caccagaggc tgtgaatagg acaactggca atttttcgac
     2461 agtcgcatac tcaaacaaac gagtgatttt ttcacctaca accgtaccca tagaagccat
     2521 gataaagtta gaatccataa tcccaagagc cacagtctga cctttaataa gagcagttcc
     2581 tgtcacaacg gcttcatgca gacctgtttt ttcacgcata gatgccagtt tcttttggta
     2641 accagggaaa tgcaagggat ccttgctttc aatccctgta aacaattctt tgaaggttcc
     2701 catatcaatc gtcaaagcca agcgttcttg ggcagaaata cgaaaggtat agctacagtg
     2761 cggacagata cgttcacttc ccagatcctt ctgatagatg gtatgcttac agcctggaca
     2821 ctgggaaaat aattcatctg gaacctctgg cttagcttga ggtttttccc taaccgaacg
     2881 attgggattg attcgaatat acttatcttt tttactaaat agagccattg attccccttt
     2941 tcggtttaaa ctcttaaagt cattttattc tttttcttga tatttaggta agaaggtttc
     3001 catcaagaag gaagtatcat aatccccagc aatgacattg cgatctgaaa tgaggtcaag
     3061 ctggaaattt gcattggtct gcactccttc aatctctaat tcatagaggg cacgttgcat
     3121 tttcatcaag gcgtcaaaac gattttcgcc gtgtactatg attttggcaa tcatactatc
     3181 ataataaggc ggaatggtat aacctggata aactgctgaa tccacgcgca agccaactcc
     3241 accacttggc agatagagat tagtaatctt acctggactt ggagcaaagt taaaggctgg
     3301 gttttctgca ttgatacgac actcgatggc atgaccgcgt aggacaatat cttcttgctt
     3361 aacagacaaa ggctgacctg ccgcaatgca aatctgttcc ttaacgatat caacacctga
     3421 aacaaactct gttactggat gttctacctg aacacgagta ttcatctcca tgaaatagaa
     3481 attgctactt gcttcatcaa gaagaaattc aatggttcct gcattctcat agccaacaaa
     3541 ctctgccgct cgaacagcag cagcacctat ttcatgacgc agcgtttttc cgattgcaat
     3601 cgagggactt tcttccaaaa ccttttggtt attcctttga agagaacaat cccgctcacc
     3661 caagtgaatc acatgtccat gctcatcacc taggatttga acctcaatgt gccgagctgg
     3721 atagataacc cgttctatgt acatggcacc attgccataa ttggccttgg cctcactaga
     3781 ggcagtttca aaggcagaaa cgaggtcatc tggtttttca accttacgaa tccctttacc
     3841 acctccacct gctgaagcct tgagcataac aggatagcca attttttcag caacaatcaa
     3901 agcttcttca gagttatgca cttctccatc tgaacctggt ataacaggca cacctgcttt
     3961 aatcatctga gcacgcgcat tgatcttatc ccccatcata tccataacat gaccagatgg
     4021 accgataaac ttgataccta cttcttcaca catggtcgca aatttggaat tttcactgag
     4081 aaatccaaaa ccagggtgaa tagcttctgc ctcagtcaag actgcagctg atagaactgc
     4141 attaatattg agataagact ctgttgcctt gccaggacca atacaaactg cttcatctgc
     4201 caaaagcgta tgaagagctt ccttatcagc agttgaataa accgctaccg tcgcaatccc
     4261 caattcacgt gccgcacgga taatacgaac cgcaatttca ccacgattgg caattaaaat
     4321 ttttcgaaac atggagaacc tccttagttc ccaattgcaa aagtaaggat accactggct
     4381 gcaagcttgc catccacttc agcctttgct tcaaccacag ctatggtgcc acgacgtttt
     4441 acaaaagtcg ctgtcataac caattggtcg cctggtacaa cttgcttctt gaacttaacc
     4501 ttgtccatac cagcgtaaaa gaccagtttt cctttatttt caggttttga taactctaac
     4561 acaccagcag tttgcgccaa ggcttccata atcagaacac ctggcataac tgggtattga
     4621 ggaaagtggc cgttaaagaa aggttcgtta atggtcacat ttttgatagc aacaatggta
     4681 tcctcgctca cttccaagac acggtccact agaagcatag gataacggtg gggaagagct
     4741 tctttgattc cttgaatatc gatcatttga tacgtaccaa tcctttacca aactcaacca
     4801 tttcttcgtt agagacgaga atttccgtta ccacaccatc cttaggagct gggatttcat
     4861 tcatgacttt catggcttcg ataattacca atgtttgacc ttttttgaca ctatcaccaa
     4921 ctgtaacgaa ggcaggttta tctggtccag cagccaagta aaccactcca acaagtggac
     4981 tctctacaag atttccctca gtagccacac ttgcttcagc tggagctgga acttcttctg
     5041 ctacagtctc tgctggagca gatgtaggag ctactggact cggtgttgct agaacgggtg
     5101 ctggagcgac ttgagttgca acttcaggca caggtctcgc ttcattcttg ctaaactgca
     5161 actcatccgt cccattttta taagaaaatt ctctcaaact tgactggtca aattgagtca
     5221 tcaagtcttt aatatcgttt aaattcatac ttatctattc tcccaacgtt tgaaagcaag
     5281 aactgcattg tggcctccaa aaccaaaagt atttgaaata gcgtatggaa tttctttctc
     5341 caagccttgt ccataaacga cattagcttc gatataatct gatacttcac ttgtcccagc
     5401 tgtcattggt acaaagttat ggcgcatagc ttcgatggtg acgatagctt ctactgcacc
     5461 cgcagccccc agcaaatgtc ctgtaaaaga cttggttgat gatacaggta cttccttacc
     5521 aagaacagct acgatagctc cactttctcc tttttcattg gcaggagttg acgttccgtg
     5581 agcattgaca taggctactt gctctggaga aatctcagct tcttccaagg ctagtttgat
     5641 ggccttgata gctccctgac cttctggatg tggagaagtc atgtggtagg catcacaagt
     5701 atttccgtaa ccaaccactt cagccaggat agtagctcca cgtttttcag cgtgttcaag
     5761 actttctaaa accaacatcc ctgaaccttc acccataaca aacccattgc gatccttatc
     5821 aaatgggatc gaagcacgag ttggatcctc tgtagtagag agagctgtta aggcttggaa
     5881 accagcgatg gcaaaaggtg tgatagaagc ttctgttcct cccaccaaca tcacatcttg
     5941 gaaaccaaac ttaatggagc ggaaggcatc cccaatcgca tcatttgatg aagagcaggc
     6001 agtattgata gatttacaaa caccgtttgc accaaaacgc atggctacat tcccagaagc
     6061 catatttggt aaagcttttg gaagagtcat tggtttgaca cgtttgggtc ctttttcatg
     6121 aaggcgaagt acctgatctt caatttcctt gattccacca ataccagatg caacgataac
     6181 accaaaacga tccctattaa gagcctctac atcaagattg gcatgattta cagcctcttg
     6241 ggctgcatac aaggcatata aagaatagtt atcaaaacgg ttggtatctt tttttacaaa
     6301 gtatttatcg aacggaaaat cttggatttc tgccgcatta tgcacatcaa agtcactatg
     6361 atcaaatttt gtaatgccac caatgccgat tttcccagtt gctaaactat tccaaaattc
     6421 ttctggtgta tttccgattg gagatgttac tccataacct gttaccacta ctcgatttag
     6481 tttcattctt ttcacctcta gctttcgcta catacttaag ccaccatcaa tggcaaccac
     6541 ttgaccagtt agataatctt ggcctgctaa aaatactgtc aaatctgcaa cctgctctgc
     6601 ctgcccaaat tctttcatcg gaatctgagc tagtgtagct tccttaatct tatctgataa
     6661 gatagctgtc atatcagact caatcattcc tggagcaatc acattgactc gtatattccg
     6721 actagcgacc tcacgtgcca cagacttggt aaagccaatc aagccagcct tagaagcagc
     6781 atagttagct tgaccaatat tccccatcaa accaacaaca ctagacatat taatgatagc
     6841 accttctctg gctttcatca tcggtttcaa gactgattgt gtcatattaa aggcaccagt
     6901 cagattgacc ttgagcactt tttcaaaatc tgcttctgtc atcttaagca taagagtatc
     6961 ttgggtgatc cctgcattgt tgaccaaaac atctactgaa cccagttctg caatagcttg
     7021 atcaatcata cgcttagcgt ctgcaaaatc tgatacatct cctgaaatgg gaaccacctt
     7081 gataccatag tttgaaaact cagcgagcaa ttcttctgag attgccccac gactgtttaa
     7141 gacaatgttg gctcctgctt gagcaaactt gtgggcgatg gcaagaccaa ttccacgact
     7201 cgaacctgta ataaagatat ttttatgttc tagtttcatt tttttccttt caaaacttct
     7261 acttatttta gtctattttt ctaaaagtgc tactaaactc gcttgatctt ccacatgagc
     7321 taagtgagca gttcgatcaa tttttttaac aaaacctgac aaaactttcc ccggtccaat
     7381 ctcgataaag ttgcttatgc ctgcttcttg catgacccca atactttcat agaaacgaac
     7441 gggttccttg acctgacgcg tcaagagctg agcaatgtcc tctttttgca tcacagcagc
     7501 ttctgtattg ccgactaggg gacaagtaaa atctgaaaaa cttacctgag ctagagtttc
     7561 agctagtttc tggctagcag gttcaaggag agcggtgtga aagggacctg acaccttaag
     7621 aggaatcaag cgtttggcac ctgcttcttg caaaagttca accgctcgat caactgcaac
     7681 cacttctcca gcaatgacga tttgtgcagg tgtgttatag ttggctggag taaccactcc
     7741 aagttcagaa gctttttgac aggcttcttc aatgacctct actggcgtat tgagaactgc
     7801 taccatcttg ccagagtcag caggagccgc ttcttccata taggctccac gcttagctac
     7861 caaggcaacc gcatcttcaa aatccaaggc gccgcttgcc accaaggcag agtattctcc
     7921 aagagacaaa ccagcaacca tatcaggctg atagcccttt tcttgcaata aacggtagat
     7981 agcaaccgaa gtcgctagaa tggctggttg cgtatagcgg gtctgattga gtttgtcctc
     8041 ttccgtatcg atgagataac gcaaatcata acctagcacc tgactcgctc gatcaatcgt
     8101 ttctttaaca atcggatact gatcatagaa atcccgtccc atccctagat actgggcacc
     8161 ttgaccagca aataaaaagg ctgttttagt catttcttac aactcctgcc cagcgagagg
     8221 cttcttcttg aattttctta gcggctccgt aatacaaatc ttttaggatt tcttcagctg
     8281 tttcttcttt agaaacaagc cctgcgattt gacctgccat gacagagcca ccctccacat
     8341 caccgtgaac aactgctttg gctagggcac ctgctcccat ttgttcaaag atttctaaat
     8401 caggatcttc ctgcttaaag gcatcttttt cagccagttc aaaatctcta gtcaactgat
     8461 ttttaatagc acgaacagca tgaccaaagt gctgagctga aatcgtagtg tcaatatccc
     8521 ttgcttttaa aattttctcc ttgtagtttg gatgggcatt cgactctttt gcaactacaa
     8581 accgtgtccc cacctgtaca gcctctgcac ctagcataaa gccagccgca gcaccttcac
     8641 catccgcaat tcctcctgca gcaataacag gaatagatac agctgtggct acctgtcgca
     8701 ccaaggtcat ggttgttaat ttaccgatat gccccccagc ttccattcct tctgcaataa
     8761 cagcgtctgc accgattttt tccatgcgtt tagctaaagc gacactagga acgacgggaa
     8821 taacgattat cccagcttca tggaaacgtt ccatatactt gcttggattt cctgctcctg
     8881 ttgtgacaac tttaacacct tcttcaataa cgagatccac gatatcttcc acaaagggag
     8941 ataagagcat gatgttgacc ccaaagggtt tatcagtcaa tgatttgatt ttatcaatat
     9001 tggccttgac aacttctttc ggggcatttc ccccaccgat aattcctaat cctccagcct
     9061 tggaaacagc ccctgccaaa tcaccatcag caacccaggc catccctcct tggaaaatag
     9121 gatagtcaat cttcaataat tctgtaatac gcgttttcat agtgcctcca accttccttg
     9181 cttacgtaat agttcgattt aaccataatt tgacagtcaa actattacct aaacaagagg
     9241 gagtgggttt ctccctactc cttctactaa tattctgctt attttgcttg ctcttcaacg
     9301 taagcaacca agtcaccaac tgttttcaag tcattttctg cttcgatttg gatatcaaaa
     9361 gcatcttcga tttctgagat tacttggaac aagtccaatg aatctgcgtc caaatcatca
     9421 aaagttgatt caagtgttac ttctgatgcg tcttttccaa gttcttcaac gataatttct
     9481 tgtacttttt caaatactgc catgatagga ctcctttaaa ataaatagtt tttttataac
     9541 aatgtgttca ccacatgatt acctaaattg taagaatgag cgtgccccag gtcaagcctc
     9601 caccgaagcc tgatagaaga acagtctggc taccatctaa agggatgaga ccttgttcta
     9661 cacactctga aagtaaaatc gggatactgg ctgcactggt attgccatat tccatcatat
     9721 tggctggaag tttggctcgg tcaacaccaa tttttctagc catcttatcc aaaatacggt
     9781 cattggcttg atgaagtagc agataatcca agtctgtcac ctctatagga gattcatcaa
     9841 tagtctgctt gatagacttg gctacatctc gaatggcaaa atcaaagact gtgcgtccat
     9901 ccatcttcaa aaatgaatct gcactttctt gatctgaaaa tggagaatgc aaacctgaat
     9961 gcccataagt taaacactcg ctgcgacttc catcgctatt gagactctca gctaagaaat
    10021 gctcttgctc gctagcttct aacaagacac caccagcacc atctccaaac aacacagctg
    10081 ttgatcgatc cgaccaatcg actgccttag agagggtttc actaccaatc accaagcctt
    10141 tttgaaagcg accagaagcg ataaactttt cagcagttga aagagcaaat acaaatccac
    10201 tgcaagccgc ggttaagtca aaagcaaagg ctttattagc gccaatatta gcttgaacac
    10261 gagcagctgt agagggcatc atcgaatctg gagtaatggt agctaggatg ataaaatcca
    10321 gttcttctcc tgttattcca gcttttgcca tcagtttctt agcaacctct gtagccaaat
    10381 cactggtaga ttctgttctt gaaatatgcc tttgtcgtat tcccgttcga cttgaaatcc
    10441 actcatcatt ggtatccata atctgagcca agtcgtgatt tgtaaccact tgctctggca
    10501 cataatgagc aacctgactt atttttgcaa aagccattat ttcaaatcct ccaaaaattg
    10561 gtaaagatta gtcaagcctt tacccatgac agcaatttct tcctcgctca tgccatcaat
    10621 aattttttct accatggcct tgtggaagcg tttatgcagt ctatgaatca agcgaccctt
    10681 ctttgtcaaa tgcagatgca ccacacgacg atcctgttct gaccgaactc gctcaatgta
    10741 gcccttacgc tctaaattgt tcaaacttgt cgtaacagtt ccaagagtta ccatcaactc
    10801 ttttgacact tgacttggag tcacatctgg agccttaccg atgacatcaa tcgtatgcat
    10861 ttctttgatg gagatatcct taaaacgact acctctcaag ttcacttcct caattacaag
    10921 gacattgtta aatatagatg ttaaatattc attgattcgt tggtagtcca tttttcatat
    10981 ccctccttct tcaaaaaact tttacaatca aaacatttga cagaaagaat tatatcaaaa
    11041 ttcaaagaat gatgcaagta ttttttattt tcctgtaaat ttaggtcttc ttctctccga
    11101 atgagctcgc actccttctt tgaaatcctc tgtttgagct aaggatttct gtaggttcag
    11161 ttctaaagta gcgtaaccct gccagtcttt aaattggctc tcccaaacca acttcttaat
    11221 ggcagcatag gaattacttg aagcacgtct taatttttta agaagctgtt ctctcgtctt
    11281 ttcaagtttt tcagcttcag aaacgcggta aaccaacccc cattctaggg ctttttctgc
    11341 cgttaaagcc tctcctgtca tagctaattg agcagcacgc gtcacaccga tactgcggct
    11401 caagagatga attccccctg catctggagc caaaccaaca ccaacaaagg cttggataaa
    11461 cttagcctta tccgtcgcca aacagaaatc tgccgcaaca gccatattcg ctgcggcacc
    11521 tgcaacagca ccgtcaactt ccattaaaac aggtttggct atttgcttga ttttataaga
    11581 aatcgtattg accaactctg cgatttttgt caatgatgga atatcatcct catccactgc
    11641 ccgcttcatc tctaccaaat ctcccccaac tgagaagacc tttccattgg cattgattaa
    11701 gataaaatgc acagctggat tttcttctgc caaagtcaga gcttctaaaa tctcctcaca
    11761 catgggaata tgaaaaccat ttgcgacctc aggacggttc aaggtaagga ttgccaagtc
    11821 ctcttcaagt tgataaataa tgtgttccat cagggctcct ttttatttta taagcagttt
    11881 atactcttcg aaaatctctt taaaccgcgt cagcttccat ctgcaacctc aaaacagtat
    11941 tttgagctga cttcgtctag cttcttagtt tgctctttga ttttcattga gtgttaaatt
    12001 atttcactgt caaagtatat cttttttagg agcagaaagc aagaaaaaat cgactgaaag
    12061 tgtctcagcc gataagaaag gctcgcttta ggctagaaag catcttattt tcttcattga
    12121 aaaaagcctt tctttctgct ataatcgtat aaaatactta ctttaggagt tcttatgaaa
    12181 gttgttaaat ttggaggtag ttctcttgcc tctgctagtc aattagaaaa agttttaaac
    12241 atcgtcaaaa gcgattcaga gcgtcgtttt gtagtcgttt ctgcgcctgg taaacgcaat
    12301 gctgaagata ctaaggttac ggatgccctg attaaatact accgcgacta tgttgcgggt
    12361 aacgatatta gcaagagcca aagctggatt atcgaccgct atgctgctat ggttagtgaa
    12421 ttgggactaa aaccagctgt gctagaaaaa atttctaaaa gcattcacgc cttggccact
    12481 cttcctattg aagaaaatga atttctctac gatactttcc tagcagccgg tgaaaataac
    12541 aatgccaaat tgattgctgc ctactttaac caaaatggta tcgatgcacg ctatatgcac
    12601 cctagagaag ctgggattgt ggtcacaagt gaacctggtc acgctcgcat cattccatca
    12661 agttatgaca agattgaaga attgacaaac accaatgaag tccttgtcat tcctggtttc
    12721 tttggtgtca ctaaggaaaa tcaaatctgt actttctcac gtggaggttc tgatgttaca
    12781 ggttctatca ttgctgctgg tgtcaaagcc gacctctatg aaaactttac ggacgttgat
    12841 ggtatctttg cagcccaccc tggtattatc caccaaccac actcgattcc tgagttgacc
    12901 taccgtgaaa tgcgcgagtt ggcctatgca ggcttctcag tccttcatga cgaggctctt
    12961 cttcctgcct accgtggaaa aattcctctg gttatcaaga ataccaacaa ccctgaccat
    13021 ccaggtactc gtatcgttct aaaacacagt aatgatgaat ttccagttgt gggaattgct
    13081 ggtgactcag gctttgtcag cattaacatg tcgaaatacc tcatgaaccg tgaggttgga
    13141 tttggccgca aggttctgca aatcctggaa gaacttaaca tcggttggga acatatgcca
    13201 acaggtatcg acgatctttc tatcattctc cgttctcgcc aactaactcc catcaaggaa
    13261 gaagaaatcc tgcgtcagtt ggttcaaaag gctaaagtag atcatgcaga aatcgaacac
    13321 gacctttcta tcattatgat tgttggtgaa aaaatgaaga gccacatcgg agtgactgct
    13381 actgcgacac gcgctctatc tgaaaacaaa atcaacatcc agatgatgtc tcaaggttct
    13441 agtgaagttt ctattatgtt tgttgtcaat aaagaccaag agaaagcagc aattaaagcc
    13501 ctctacaatg ccttttttgg agaaagtaag gaagactaag catggcccaa tcacttaaca
    13561 aaacagtact cctcagtacg acaggcactt cctacctctc tatcgctggg aaagttggga
    13621 aattccttgt cggagatcag gctttggaat tttacccaga tgtcaatgtc gaacaattta
    13681 tccagattcc ttggagccat atcaatcaga ttggagccaa tgtcactgga cgcaaaatca
    13741 gccgccactt cgaagtcttt acagacaaag gaaaattcct ctttgcttca aaagactcag
    13801 gtgccattct caaaattgca cgcgaaaaac taggcaatga caaggtcgtc aaacttccga
    13861 ccctgattca gacaattagc caaaaattta aaaatctatt tgcaaaaaag tagaaacttt
    13921 agtataatca tagcaacgga taagtaggtt atcttcttct ttcagaaagt ctgcgggtac
    13981 tgcgagcaga taggagggat aggtgaaatt ctaccgttag taacaattac atacacaatc
    14041 aagtgcacac ctgtgaagtt ggatggaacc gtggccctgc cactccaacg ttttgtcagg
    14101 cgtgcttttt tcataaagga gttcttatgt tagatatcaa acgtattcgt acagattttg
    14161 aagctgtcgc agaaaaatta gctacacgtg gtgtagatgc tgctgtcttg aatgaaatga
    14221 aagaaatcga tgctaaacgt cgtaacatct tgatcaaggt tgaaactctc aaagcagaac
    14281 gtaacacagt ttctgctgag attgcccaag ctaagcgcaa caaggaaaat acagatgaca
    14341 agattgctgc catgcaaaat ctatctgctg agattaaagc cttggatgct gaattggcag
    14401 aaatcgatgc taaattgaca gaatttacaa cgactcttcc aaatatccca gctgacagcg
    14461 ttcctgttgg ggctgacgaa gacgacaatg tggaagttcg ccgttggggt actccacgcg
    14521 agtttgactt cgaacctaaa gctcactggg atctcggtga agaccttggt atccttgact
    14581 gggaacgcgg tggtaaggta acaggcgctc gcttcctctt ctataaaggc ctcggtgctc
    14641 gtttggaacg tgctatctac aactttatgt tggatgaaca tggaaaagag ggctatactg
    14701 aagtcatcac accttacata gtcaaccatg attctatgtt tggtactggt cagtatccaa
    14761 aatttaagga agatactttt gaactcagcg ataccaactt tgtcttgatt ccaactgctg
    14821 aagttcctct gacaaactac taccgtgatg aaatcttaga cggcaaagat cttccaatct
    14881 acttcactgc tatgagtccg tcattccgtt ctgaggctgg ttctgccggt cgtgatacgc
    14941 gtggcttgat ccgtttgcac caattccaca aggttgaaat ggtcaaattt gccaaaccag
    15001 aagaatctta cgaagaattg gaaaaaatga cagccaacgc tgaaaacatt cttcaaaaac
    15061 tcaaccttcc ataccgtgtc gttgctctct ctactggaga tatgggcttc tcagctacga
    15121 agacttacga cttggaagtg tggattccag cacaaaacaa ttaccgtgaa atctcaagct
    15181 gttcaaacac agaagatttc caagcccgtc gtgcccaaat ccgttaccgt gatgaagcag
    15241 atggcaaggt gaaactcctt cataccttga acggttctgg acttgcagtt ggacgtacag
    15301 tggctgcaat tcttgaaaat taccaaaatg aagatggttc tgtgaccatc ccagaagcac
    15361 ttcgtccata catgggtgga gctgaagtca tcaaaccata aaaaataagg tttagctatt
    15421 tctagctaga ccttttttcg taaccaaatc agataagcac ctagtacaaa gaataaaata
    15481 gttaggcata taatggtttc agccaatacc aggtaatcca gaaatggaag tttcaaaatt
    15541 ccctgagcca tcttgagcga ggtcgctgtg ataatggttg ggaaggtgag ggctgagaag
    15601 gctggttgaa aaccttgttt taaaatgttg ggcagacgag ttaaaacaaa gaaaaagaag
    15661 gattgagaag ccaaaatcat gacaatcaag ccccaagtcg gcaggctggt tcctcctact
    15721 cgaactagag aagccaagag tagagagaaa ggagcacagt agattccttc ttgtccaagc
    15781 aaggctagtg ggagtggatg tttctttaaa tcgctataaa taaggggata gagatagaag
    15841 gtcaagagaa aaccaaaact caaggtcgca taggcaattt cgataatacc taccagagga
    15901 taggtcaagg cagccactgc tatccccaca tagagaaccg tccaacttgg agtggcatga
    15961 accctccgcc ctggacaagc aaacttgatg gtaaaaccag caatcaaggt caaatccaag
    16021 agaaatgaaa accaccaaat cccttgtgct accaaaggaa gataagagaa tacgcgaaag
    16081 acataggtcg ataaaatcat cccagccata ggaaaggttg ccattcctga caaaagaggg
    16141 ggcttggtca attcttgctt ggtttctttc caattaaaga gatgcagaat tagaaagtaa
    16201 atccataaaa ccaaaccagt cagactaaaa agatgggata gaaccggcaa cgtatctaaa
    16261 ataagatttc cagctcctgc caaacctagc aaacaacctg aaaatactaa ggggagtttt
    16321 ttcatcctaa cctccaataa tcatgttagt ttcagtataa cataaaagcg cttaaatgag
    16381 gatttaaaaa aacgaagtcc gcttatttca gacttcattt tactcagata tgaattaggc
    16441 ataaggttgc aattctggat taattggtgt attagctaag ttgttggcat agttacagag
    16501 gattgctagg ctgacaccaa aaaccacatc caaggcattt tgttgagtgt agccagcttc
    16561 taaaaactca gacaaggctt catctcctac acgacccttg gtattgataa ctgccaaggt
    16621 aaacttagct agggtatcca atttaggatc tgtttcaatt ggagtacgat tgcgaagagc
    16681 ttgaatcaag tcatcattca tctggatttg tttgatggaa aaggctgtgt gacctgcgac
    16741 acagaaggca caaccattgg tcacggctgc cgtgatttgc accacttcac gctcaacggg
    16801 tgtcaggctg ttgcgacggt ggatagatga gacaatttgg taggcttcta aaacagtcgg
    16861 ggcattggcc aagagaccga ttaggttggg aatatagcca ttgttgtctt tttctactgt
    16921 ttcaagaatt tctttcactt ctgctggtgc tgactctact gtatggatag taaatgttgt
    16981 cataagatac ctcttttctt attattgaca ctaatattat tggaaaatct tataaaatcc
    17041 tgattcctaa gtttatctaa gataaagctt tattctctca taagattttc gttgttatat
    17101 tagtttatca cacttccaat cacttgcata atatatatta tatatcaggc tgataaaaat
    17161 tatttatagg caaaaaaatc acacgagctg tgtgattcca ttatttgtca aaatactttt
    17221 tagtttcagc aataacgact ggcgacaaga ccaagagggc aatcaagttt ggcagagcca
    17281 tcaaggcgtt aacgatatct gcgataatcc agaccatatc caactcgata aatcctccta
    17341 acaagaccat gagcacaaaa accacacggt agagccagat aaagcgaacc ccaaagagga
    17401 actcaaaaca gcgttctccg taatagttcc aacctagaat cgttgtaaag gcaaaaagca
    17461 caaggaagat ggtcaagagg gcagggccaa agtgtgaaaa gactgttgag aaagctgact
    17521 gagtcaaggc aaccccattc aagtcaccac tccaaactcc agttaccaag atggtcaaac
    17581 cagttagagt acaaatgatg agggtatcaa taaaggttcc tgtcatggaa atcaaacctt
    17641 gctctactgg ttcatttgtc ttggcagctg cagctgcaat aggagcagaa cccagaccag
    17701 attcgtttga gaacacacca cgcgccacac cattttgaat agccatccga acgctagcac
    17761 cagcaaatcc acctaccgca gcaaggggac taaaagctga ggtaaagact aaagcgattg
    17821 tgccagggat ttttccgata ttaaagaaaa taactgtaag agttcctaag atataaatga
    17881 tggccataaa aggaacaaca gtagttgaaa ccttagaaat agacttgagt ccaccaaaga
    17941 ctgcaatcgc tacaaagaca gacaagacga gagctgtgat ggctggcgaa atcgtcgttg
    18001 tattttggat agattctgta atcgagttga cttgggtgaa ggttccgatt cccaagagag
    18061 caaccaatac tcctgctact gcaaacaaaa cagcaagtgg tcgccacttt tctcccatcc
    18121 ctagaaggat ataatgcatg ggacctcccg ctactgcacc atggtcgtcc ttggtgcggt
    18181 atttgatggc caagagtcct tccgcatact tggtagccat tccaaagaaa gccgccatcc
    18241 acatccaaaa tagagctcct ggtccaccaa ccttgatagc cgtcgcaact cctatgatat
    18301 ttcctgttcc aacagttgat gccaaggctg tacacagagc tgcaaaactg gatacatcac
    18361 catgtccctt atcctggata aaaataagct gaaaggcctt gggcagacgc aaaacctgca
    18421 agagtcctag ccgcatggtt aggtaaatcc ctgttccgac caataaaatc aagaggggcg
    18481 gtccccaagc aaaatcatcg attgatttaa gcaattctaa catttccttc tcctatcgtt
    18541 tcaaccccaa aagaaagagc acatgcaaga tacatgtact ctggaatgct tagataaatg
    18601 ctaaaaagcg gtctatccta gctctgtcct tttacctgag agtttgagca gttgcctgcc
    18661 ttgccccttc ggtgccttta cggtctctcc agagttccgt ccatttacag tcatggaaaa
    18721 tcaaacgatt ccccacttct attaaacttc attcggtgtt ggtatttaat tgattctaat
    18781 tttacaaaaa atgttggctt ttgtcaatgt gtttattagt aaaaattagt tcaacagttt
    18841 ttactttata aagtccagaa tactgctatc ctttaaaagt gacaatagtc gcaccactgc
    18901 ctccagcatt ttgtggggca tagccgaaac tcttgacatg tttgtttctt tgcaagtatt
    18961 tggtaactcc ttcacggatg actcctgttc cgataccatg gatgatatca acttgagcca
    19021 tattgttaag caaggcttgg tcgatgaagg catctagctc attcatggct tcttcatagc
    19081 gcttgcctcg aagatccagt ctagcttgag gtcctcgccc agaagttcgt ttcacaacat
    19141 tgacctgttt cttcttgact ggtttttctt gctgggcttg aacaagatca aactctttct
    19201 cttccaaggt catcttaatc aagccaactt gggcttccca gcgaccgtcc ttgagttgac
    19261 tggtcaaggt accacgctga ccataactga gaaccacgat atcatctccc acctttggag
    19321 ctcgtttttt cttggccttt tgaaggacct tatttttaga caagtccact ttttcaggag
    19381 ccaatttttt caacttggcc ttggcttcaa tgatttcgtg gggcttgagt tgggatttac
    19441 tgtggagatt tttgagaatc tggtcacttt cacttagggc catatccaca atctcagcag
    19501 cctgttcacg cgccttgtta agctcggttt ccttttcacg attaagctcg ttgtagagtt
    19561 tttttagcgc acggttcatc ttgagatttt cttgctccac ctcacggata ttgtccaagc
    19621 gtttgcggct ttccagcgtc tgctcttcta attgctcaat gatacgattg acgtcattgt
    19681 cctgatcgat ctgctgactg gcatctccta cgataacttc agataggcct agacgtttgg
    19741 caatttcaaa ggcattactt cggccaggaa caccctgcat aaagcgatag gtcgggcgaa
    19801 gagttgcagt atcaaactcc atactggcat tttgcacaaa ggctgtctca ataccgtagg
    19861 ccttgagttc tggatagtgg gtcgtcgcca tggtcttgat ttgacgcagg cgaaggtcct
    19921 ccagaatagc catggcaagg gctgctccct cttggggatc agtaccagcc cccaattcat
    19981 ccaaaagtaa gagtgaatgt tggttgacct tgccaagaat atccacgata ttggtcatat
    20041 ggctagagaa ggtagacaag ctctgctcaa tagactgctc atctccaata tcagcaaaaa
    20101 tttcttcaaa aataccaaca cgacttccct tgtctgctaa aatcggcaat cctgactggg
    20161 ccatgacctg tgtcaagccc agagttttga gcatgatggt cttcccacct gtattgggac
    20221 ctgtaatgac aatagctgtt aaatcttgac caaaatagac atcatttgcg acggcatttt
    20281 tgaccaaagg atggcagaca tggagcagtt gaatctcttg attttctgac agctgaggca
    20341 cgactgcttg tctttcttgg ataaatcgaa ccttggcacg aatcaagtcc agatgaccga
    20401 taatccaagc atcattagca atctcagccg catgagggcg gacacgctca gaaatttctt
    20461 ggagaatgcg aagcatttca tagcgctcat ctgctcgcag actagcaatt tcttcgctca
    20521 gtttgactac ctcacggggt tcgatataga cggtgtttcc actagcagaa atatcatgaa
    20581 cgacacctgc aatcttattg cggtaggtgt ttttgactgg taaaacctta cggccatttc
    20641 tgctagcaac aattccttcc gtcaacatct gcgctttttg cttgagcaag tcttgtaaaa
    20701 catcgcgtac ctgactctcg ctatcatgta tttttcgacg gattcgcgcc aattcttcac
    20761 tggcaaaatt ttcaatgaaa cccgcatcat taaaggcctg aagatttcct tgtaattgcg
    20821 gaaaatcatg taatttctca aaccaaaggg ctaattcttc caagctgaca ttttccagat
    20881 tggcgtaaaa actttgaagt tctcggctgg caagaagcac gcgtttcaag agtaggaact
    20941 cctcgatatt gagatccgct cccatctcca acctcttgca gactcctgca atttccttag
    21001 ttgagagaat agtaaaatgc ggttgctcga cgaaaagagc ctgcatttcc ttcatctcag
    21061 caaaagcctg tttgatttta tctgctttgg cagtcggagc cagttgtctc aattgctcca
    21121 agccctgctc ggtcaacaaa tgaggctcaa acaaggcctt gaccttatcg aactctaatg
    21181 tttctaatat tttcttattc atctttattt ccttgtcttt gaatgtctag gtgtggtagc
    21241 tttttacatt ctgatagatt caagtcaaat ctgtaaacaa agggctagga aaactcctgc
    21301 cctttttatc cgattaaatt tgtcacccag atttgtttga gccaactggt tgttatcggt
    21361 atgctctgga tgatgtgttt tgcgacgata ctcttttcaa gaggattttg tataactgcc
    21421 atggggatgg tcgccaagat tgtcaaggcc atttgcaaga caaataaggt caccaacatg
    21481 gacaagatac ctgctgaaac ttggaacaac ttaccaccca gttttttgct aggaatcaag
    21541 tgtaagagaa gaccaagcaa acgaccaatg ctatagacaa tcccaaatac aagcaagtag
    21601 ccgatacctg cataaaagac cttatccagc tgaaagagtt gatccgatgg gaaaaagaaa
    21661 gtcccctgac cttcctgcga atttgcataa gggagcaata aatggaattg ctctccaagc
    21721 cccttataaa actgaccagc cataaaagcc gatgccatgg ctgaaatcag gtaataaatc
    21781 tgtaagagca ggcctctccg atagccgata taaaatcccc aaaccaagac caatagaaga
    21841 aggaatgaaa tcataaggaa tcctcaatct tgctctgttc ttgcttacaa gtcacaagct
    21901 tgtgacggag ttcttctagc tcttgctcct tatcgtcaaa ttcaatctca cggctgagct
    21961 gagttgataa acagttgact gccaacaaaa gagcgattgt ttcatcatct gcgctaggca
    22021 tttgttcttt aattgcttgg tatttttctg tcgcaacctt agcgatttcc tccataaaaa
    22081 ggttatcatg ttcacttgtc aaggttaacg attttttccc gaatgtaaat ttgaatcgat
    22141 ttagatttgc cataaaattc acctcacgat attataccaa aattcgctaa ttttgtcagt
    22201 ttttacaaat tttactgctt ttgtggtaca atagaaacta tggcaagtat cacactcaca
    22261 ccaagcgaaa aggatattca ggcttttctt gaacactatc aaaccagtct ggctcctagc
    22321 aagaatccct atatccgcta ctttttgaaa ctacctcaag caacggtttc tatctatact
    22381 tctggaaaaa tcttgcttca gggtgaaggg gctgaaaaat acgccagttt ctttggctat
    22441 caagctgtag agcaaaccag cggacaaaat cttcctttaa ttgggacaga tgaggtggga
    22501 aatggttcct actttggtgg gcttgcagtt gtggctgcct ttgtcacacc tgaccagcac
    22561 gactttttac gaaaactcgg tgtgggagat tctaagactc tgaccgacca aaagattcgt
    22621 cagattgctc ctattctcaa ggaaaaaatc cagcaccagg cactccttct ctcacccagc
    22681 aagtacaacg aggtcatcgg agaccgctac aatgctgttt cggttaaggt tgccctccat
    22741 aatcaggcta tctatctcct ccttcaaaaa ggtgttcagc ctgagaaaat tgtgattgat
    22801 gcctttacca gtgctaaaaa ttatgacaag tacttggcac aagaggccaa tcgtttcagc
    22861 aatcctatca gcttagaaga aaaggctgaa ggcaaatact tgtctgtcgc agtttcttct
    22921 gtcattgcgc gtgatctctt tctggaaaat cttgaaaatc tgggacgaga actgggttat
    22981 cagcttccaa gtggagctgg aacggcttct gacaaggtgg ctagccagat tttgcaagcc
    23041 tatggtatgc agggactcaa cttctgcgcc aaattgcact ttaaaaacac tgaaaaagcg
    23101 aaaaaacgct tagaaaggta agttatgaat tcatttaaaa atttcttaaa agagtgggga
    23161 ctgttcctcc taattctgtc attactagct ttaagtcgta tctttttttg gagcaatgtt
    23221 cgcgtagaag gacattccat ggatccgacc ctagcggatg gcgaaattct cttcgttgta
    23281 aaacaccttc ctattgaccg ttttgatatc gtggtggccc atgaggaaga tggcaataag
    23341 gacatcgtca agcgcgtgat tggaatgcct ggcgacacca ttcgttacga aaatgataaa
    23401 ctctacatca atgacaaaga aacggacgag ccttatctag cagactatat caaacgcttc
    23461 aaggatgaca aactccaaag cacttactca ggcaagggct ttgaaggaaa taaaggaact
    23521 ttctttagaa gtatcgctca aaaagcccaa gccttcacag ttgatgtcaa ctacaacacc
    23581 aactttagct ttactgttcc agaaggagaa taccttctcc tcggagatga ccgcttggtt
    23641 tcgagcgaca gccgccacgt aggtaccttc aaagcaaaag atatcacagg ggaagctaaa
    23701 ttccgcttct ggccaatcac ccgtatcgga acattttaag aaacctaaga ggccgagaat
    23761 caccaatctc agcctcttct tctatcgtga gaagtgattg tttattgaca aatactcaat
    23821 gaaaatcaaa gaacaaacta ggaagctagc cgtaggctgc ttaaaacagt gttttgaggt
    23881 tgcagatgga agctgacgtg gtttgaagat attttcgaag agtataaaat aaaaacaatc
    23941 ccaactctca cctattcatg caaaggaatt ttatggaagt ttatttttca ggaaccattg
    24001 aacggattat ttttgaaaat cccagcaatt tttatcgcat cctcctccta gaaatcgacg
    24061 atacggacgc agaggatttt gatgactttg aaatcattgt cacaggaacc atggctgatg
    24121 taattgaggg cgaagactat actttttggg ggcaaattgt ccagcactcc aagtatggag
    24181 aacaactgca aatcagtcgt tatgatcgcg caaaaccaac tagtaagggc ttggtcaagt
    24241 acttttcaag tagccatttc aaggggattg gtctcaagac agctcagaaa atcgtggata
    24301 cctatggcga aaataccatt gacgaaattt tgcaacaccc agaaaagtta gaaggcatcg
    24361 caggactctc tgccaaaaat cgcgaggctt tcgtctccac tctccgtctc aactacggta
    24421 cggagatggt tttggccaaa ctagccaact acggcattcc caacaaacta gcctttcaga
    24481 ttcaagactt ttacaaggaa gaaacccttg atgtggttga aaattatccc taccaactgg
    24541 ttgaggatat caagggtttg ggctttacca ttgctgacca actagctgag gaactaggca
    24601 tcgaaagtca ggctcctgaa cgcttccgcg ccggtctagt tcacagtctt tttcaggcct
    24661 gtatggaaac aggagacacc tatgttgaag cacgggattt gctggaacaa acccttactc
    24721 tccttgagtc ttcccgtccc gtggaactag accccagcca agttgcccaa gagctctcct
    24781 acctgatcga agaagacaag gttcagcaga ttgataccaa gatctttgac aacagcctct
    24841 ttttcgctga ggaaggcatc cgcagtcact tggttcgtat ccttgaaaaa ggagaacaga
    24901 agagtcatga tttagaaacc attcaaaaac atatcactac tatcgaggaa gagctgggaa
    24961 ttgagtatga taacattcaa aaacaggcta tctgtgatgc tatccagaat aaggtcttta
    25021 tcctgacagg tggacctggt actggtaaga caactgttat caatgggatc atcgctgttt
    25081 acgccctgtt agagggactt gacctcagga agaaaagcaa tctgcccatt cttcttgctg
    25141 ctccaactgg acgagccgct cgccgcatga atgaattgac aggtttgcct agcgcgacca
    25201 tacaccgcca cttgggaatg acaggtgacg atgataccag tcatctggaa gattacctag
    25261 atgctgactt tatcatcgtg gatgaatgct ctatggtaga tacttggctg gccaatcaac
    25321 tcttctccaa tatctcttct aacagtaaga tcctcatcgt aggtgacagt gatcagctac
    25381 cttctgtcag tcctggacag gttctagcgg atctgcttca tattcctttg attcctcaga
    25441 ctcgcttgga aaaaatttat cgacaaagca aagaatcaac catcgtcacc ctagctagtc
    25501 agattcgaca gggcatcttg ccagctgatt tcacccaaaa aaaagctgac cgttcctact
    25561 ttgaaattgc tagtggccat attcctgcca ccattgaaaa aatcttaggt gccgccctca
    25621 gaaatggtat tcctgcccgt gatatccaag ttctggctcc tatgtaccga gggacggcag
    25681 ggattgatgc tatcaatcag ctcatgcaag acctgctcaa tcccccacaa aaagatcaac
    25741 tcagttttga agctcctcag tgccactatc gtaagagaga caaggtcatt catttggtta
    25801 acgatgctga aatcaatgtc tttaatgggg atttaggagc tatcacagac ctgattcctg
    25861 gtaaatacac cgaatcgaaa caagacgaga ttgtcattga ttttgatggt aatgaagtct
    25921 cttacccccg taacgaatgg tacaagattc gcttggccta tgccatgagt attcataagt
    25981 ctcagggaag tgagttccca gttgtcatcc taccgattac tagtgctagc cgacgtatgc
    26041 tggagcgaaa tctcatctac acagccatta cacgtgccaa aagcaaactc atcttactag
    26101 gcgaattaca agccttcgac tatgctaccc aacacatcgg aactgcccga aaaacctatc
    26161 tgattgaacg cttcagtgat ctactggaga atgttgaaga aaagcaacaa gctgtctctg
    26221 aaacagtcac atcaagtgcc tctgaacaat cctacatcct aaccgaagaa aactgggaca
    26281 gcattccagc catgattggg attacagaca cagacctcaa agagattttt ggaaaatagt
    26341 acatcagaaa acccaccaat tcaggtgggt tttctgttta ttaagattat tttactgttg
    26401 ctgtgcttgt gatcaattca acagcttttt tgatagtgat atcatgtttc aacatgtcag
    26461 ctgaaagcaa gttttgaact tgtgcaactt ccatgttgta gtctgctgcc aattgctcaa
    26521 cttctttttg gatttcttct tctgaagcat caaatccttc agctttggca actgcttcga
    26581 taacaaggtt agtcttagta cgtgactcag cttctgcttg gtattggttg tgaaggtctt
    26641 cttgagtagt tccagtgatt tggaagtaca tgtcagggtt gatcccttga cgttgcaagt
    26701 tcccaaggaa ttcatttact gaacggtgaa cttcttcatg gatcatttct tctggaagtt
    26761 ctacgatttc agcgttttct acagctgtat caattgctgc accttcaact gcatctttgt
    26821 aagcttcttc tttagcagca gccaattctt tgcggtattt ttctttcaag tcagcaagtg
    26881 tttcaacttc ttcatcaatg tcttttgcaa gttcatcgtc aagagctgga acttctttag
    26941 cttttacttc gtggatagtt gtcacgaatt tagcttcttt acctgcaagg tcttctgctt
    27001 ggtagtcttc tgggaatgtt acgataacat caacggtttc gccagctgag tgacctacca
    27061 attggtcttc gaaaccaggg atgaattgac ctgaaccaag tccaagtgag aagttttcac
    27121 cttttccgcc gtcaaattca acaccgtcga tagaaccaac gaagtcgatc acaacagtgt
    27181 cgccgttttc agcagcagct tccttgataa ccaattcagc caggttgttg cgttcgcgtt
    27241 cgatacgctc ttcaacatca gcgtcagtta cttctttttc tacatcaact gatacttcaa
    27301 ggtttttgta gtcacccaat tttacttcag gttttgtaac gacttcagca gcgataaccc
    27361 agtcttgacc tttttccatt gaagttacgt caatttttgg ttgggcaacc acttcaagac
    27421 cagcttcttt tacagctgct tcataagcgt ttggcaaaag agcgttcata acgtcttggt
    27481 aaagtgattc ttcaccaaat tttttgtcga agataggacg tggaaggtga cctttacgga
    27541 aacctggaac attaagagat ttcttcactg acttgaagac acggtccaat tctggtttga
    27601 tttggtcttg agagatagtg aaagtcaaga caccacggtt tgtttctttg ttttcaaatg
    27661 atacagacat tctgtcattt ctccttaaaa ttttttaaat acagtctatt ataacatttt
    27721 ctataaataa aatctagact aatgcagcca attatagaca acaagccttt ttcctaacta
    27781 aaacactcat aaagtataga caattttaga ctactttgga tatcttttgg ttagtcattt
    27841 tccaaaacta atttatctaa tttttcagtt atatagtaga ttgaaactag aatagtacac
    27901 ctctacttct aaaacatttt tagaaatcga tttgactgtc ctgatcgatt tgtcctgttc
    27961 ttgtttcatt ttactatata gatcgcattc gaaagaattc gttccaaaat tgcttggttc
    28021 gtttttctct tggacgacta aagaattgat gggcattatt tttttcaaca attttcccac
    28081 agtccataaa aataacatga tgggctacat ctctagcaaa acctatctca taaagctgat
    28141 caaaaactca atctgaggag aaaatccaat aaggctttcc ataataaagg acatcctatc
    28201 aagatttttg aacacccgat aggctgcctt tttccgattt tgaagacttt ttgtccagct
    28261 tcttttacct tataaataac aacaaaaatc aaagacaagc tagaaagatt accgcagata
    28321 actcaaacag tgttttgagg ttgcagataa gactgacgaa gtcagtaaca tatgcctaag
    28381 acaaagcgat gctaacctga tttgaagaga ttttcgaaaa atattaaata tactctacaa
    28441 tttgatcaat caattcttgg tcgtctaaac ctgtaacttg tataacaaca tctttgactg
    28501 attgtttagc caacaattca cctaatcgaa tactttcttc atcatttaca tcgcgatagt
    28561 caaaggcata gccaactgtt ttaagtaggt ttttatatga caaactgagt tctttcaatt
    28621 cacgtatcgg ccggatgaat cgttcattat agcctaattt tcggattgga gtacgagcta
    28681 cgcgactaac ctcgtctact atgaaagggt tttcaaaccg ttctataatg actttgtgat
    28741 aattctccaa ttctttttta tcaaagttcc atttggcaat caagagactc cgaatttcag
    28801 ctaatacaga ttcaatccga gatttgatat taggattttg aagagcttcc aaaattgtct
    28861 tggcaccata atgcgcacca atgtaagctg aagttgcatg tccagaattg actgaaaaaa
    28921 gttttcgctc aataaagggt tctaaatctt cttcataatg cacatctttt agacgtaaat
    28981 ctggattttt aagacgcttg gtttccacga cccattcatt aaagggctcg accacaacaa
    29041 aaagggaatc ttcgtgactt tgtgctggaa caatcctgtc tactgcagca tttggaaaac
    29101 ctatgtagtt atcagcaaat gtcaaacctt ccggacttaa atatttcttg acttcttgat
    29161 aaagaaattg agacccgcca atcatatttt cacaggccat aacatccaat acctgtgtat
    29221 tccctgcaac tcggcgagct tcgattcctt tggctagaag ttcagcgata aaagggagta
    29281 tattaggtcc gattgcagta gtaataatat ccgtcttttg aatcgcttca atgacttgct
    29341 caggatgttc tttgctatta atgccagcca cattagttac ttctatacga gactgtcctt
    29401 tctgtgcaat ttcaatttca tacttgccct tttcatttag agcatgaatt atctgattat
    29461 tgacatccac aaaatcaata tggaaaccat ttttaaatag aatttcacct ataaaaccac
    29521 gaccgatatt accggcacca aaatgaacag aatgtttcat tcaatttctc cttttcctat
    29581 ttgagggaca tatatactag atgacaaatg acgaaattca attattcaac agcatcaaat
    29641 aagcgcaata cttcctcttt tgactgagca tctgctagtt taagaacatt atccacatct
    29701 gcacagaaga tagaaatttt ctgaataatt tctaagtgtt cattaccaat accagcaata
    29761 ccaaaaagaa ccgttgccac ttgagggtta gatacattcc caaaatcaac ccctctaggg
    29821 acttgaacga ctgtaatacc agacttaagg acatcatttt ttgctgcatc tgttccatgt
    29881 ggtatggcga taaagttacc catgtaaaca gatagctctt tatctcgctc aatcatggct
    29941 tcaatatagt cttcattaac atatccaccc tcataaagaa cttgcccaca ataacaaata
    30001 gcttcctcct tgttagaaaa ttgtttatta agcttaatca aatgtttttc aagtttcata
    30061 ttagttctcc aatttcttaa ttttttcgtt aaaaatagta tttagcatct gataaataat
    30121 ggattgattt cccgtcttgt aaatctctgt ataaagatga ttttcaataa tcgactgact
    30181 aatagctgtc attaaatctc tagcttcctc gctagcttct ttagacatta gcattagcag
    30241 acaccttttg acctcttctt cctctcgttt cattgacaat gcggagatag gtagtttgag
    30301 ttcaaacata gtaaaactat ttgttgttac tttgctagac tgactatgta aaagcaccag
    30361 accagtatta ggaatagcca taggactatt ctggaagtga gataacaatt ttcttgttag
    30421 gtaatctctg tcactcacat tcttctgata ttggatgatt tgttctaccg tgtgctcaaa
    30481 tgattgatta ttctccaatt gaaccaactc gaattgactc aaaagctgac tactagatat
    30541 taaataatct tgcaagtcat aacttttctc tggagcgata gcatcacgcg cgacaattgt
    30601 acgatgtgcc tgtacatact gaagtcgttc ctgtaattca aacaattctt tgacggttgg
    30661 aaaactagaa attacatcga tttcctgatt agtcagcggc ttggtagata aaatataatc
    30721 atactgactg agatcaatac tgtggtaatc tactagagac tgaatatcta tcaactctac
    30781 aaaaggagca acactcttaa ttttactgac taataaatct ctagtgaccc gacgttcatc
    30841 cgttaaaagc aaaacacgga ctggataaat ctctggacta cggcctaagc tagagataaa
    30901 atgtagggca atcatgccat actcatactc tgtatgaaga tatttcggaa aaatatcatt
    30961 cactaaaaga ctgatgactg tataaagaaa tttattcctt tcaactaaaa tctggacaga
    31021 ttctggcaaa ttttcaccct gaaaaaggat agggacgcct aaactgagcc gaatatgatg
    31081 gaaaagaaaa ttgaataaaa ccttgtcctt aaaaaagtca atcttggtat acatggaaac
    31141 cgtatcaatc agatttgaaa tattgtagaa aaattcacca tcaaatttct ccgtaaagag
    31201 cggattgtcc tgacgtttaa tgattaattc atccaagatg ctcgcaaaat agataatttc
    31261 ctgaatacta taaaattgtg cagtctgctt agagtaagct tgaaaaattt tttgagaaat
    31321 ttccaaagcc tgcttactag tattaggtga attttcaatg ttttgctcct gacctataag
    31381 agataacaag atcgcaaaaa acatcttcat cctagcatcc atatctggaa aacctgacag
    31441 ttgcttatta acaatctgac tggccagcct agttctatct gcttctaaaa tatcaaagct
    31501 cccaaaatta ccggttgaaa aatctgctac tgagatacag tttgtcagta aaatagccaa
    31561 aaatcttctc ttaccaactg aatcaccaga aatccgataa cctttttgtc gttcaatttt
    31621 cagatcaaag tctaaaagac gcttatcaat atctgaaata tcctgaataa tagttacatt
    31681 actgactttc gtgcactctt gcaatgcttc attggtgaca aaaccactct cagtcagaag
    31741 gcgataggta atcaactctt gtctttcttg gggactatac tcaactaaca cttcaagttc
    31801 tgtcggcaaa tcatccaact ctccagtcaa aatatagtat ttcccatgct tcgttatttc
    31861 gattccatat tgctccatgc tatctgtcaa atctgataaa gttcgataaa ttgttctaga
    31921 tgaaacctgt aagatttcag tcatatcttg cattgaaagc ttccctacat gtaggaaagc
    31981 cttcaataat tgttcttctc gtttggttaa tagcattcgt aatttagaaa cctctagttc
    32041 tatttgtaca ttctagcagc cattttgtca tattctggtg ttgttactaa actatccaca
    32101 ataagaaatt gagaattcgg tgctgcttgc tgcacttctg cctgtaaaga aatagttgtt
    32161 acaatcatta tgtttttaga attaaattca cctaattctg aaattttggc agtagaaact
    32221 ggaatacgaa tattcttgtt tctaaaaata gcccgaatcg tttcacagcc catagttgca
    32281 gttccctgtg ctttaccata agcaactact acagcatcaa tatggttaag gtcaatttcc
    32341 tgactatcta ctggtgctga agttggtata tctccttcaa tttctgctat tggagaagct
    32401 cctgttaatg aagctacaat ttcatcataa cgaggggacg ctaagaaatt atcgacagaa
    32461 acatgaatag cacttggact cttgtcttta gctcttggtg tcagttcttc ctgagtaaca
    32521 attaatgttt ttggtgtatc aagcaaattt gagattgcct gattagatac tggaatctct
    32581 agacctgctt ttttaacctt atctcgaaga atactagctc ccatagcaga gcttcccata
    32641 ccagcatcgc aggcgaaaat gattttttcc actgagtctg tcaaaacaac tgtatcaaca
    32701 gaagttgata ctaactgacc tttagactga gccttagctg cttgggtagc cgcctgagca
    32761 gcttcgagcg aatcctcagt tgacttgtct gcatgaagaa taagggctgc tacaaggaaa
    32821 gaaacaactg ctgctactaa aacacctaga agaacattta tatggggcca aacacctttt
    32881 ggcgccgtag ccataatcgc aataatagaa cctggtgaag ctggagattt aagaccagcg
    32941 tctaagagtt gaaaagtaaa agttccagag atacctcccg ccatagcagc taaaaataga
    33001 gtaggcttca tcataacata aggaaagtaa atttcatgaa tccctccgaa gaaatgaata
    33061 accattgccc cccaagaaga agatttagca gaacctttac cgaatacagc ataagctaat
    33121 agaattccca ggcctggtcc aggattagct tccaatagga agagaattga cttaccagct
    33181 tgagctacct gttctactcc cagaggagta aaaatgccat gattgagggc attattgagg
    33241 aaaaggactt tagccggttc gatgataata ttagccatag gaaggaggcg agcattgaca
    33301 atagcctcaa caccattccc aacagctcca gtaagagtcg atacgactgg accgattgcg
    33361 tagaaagcca aaagcaataa tgcaaaacca acgagaccag ctgagaagtt attaactaac
    33421 atttcaaatc cgggacgaat tttttcttgg aacttctcat caaatttctt gatagtccat
    33481 cctcccagtg ggcccattac catagctccg ataaacatag gaacactaga acctgtgatt
    33541 gcaccaacag tagcaatagc tcctacaacg gcaccacgtt ggccatggat catatatcca
    33601 cctgtgtaac caatcaggat tggcaataaa tacgttaaca taggaccaac aacagtagct
    33661 aactgttcat ttggcagata gccatcagcg ataaagaggg cagtcaatac tccccaagca
    33721 ataaatgctc caatattggg cataaccata tttgaaagcg atgtccctag tttttgaacc
    33781 ctgactttca atgatacttt ttcttccatt agattatcct ccttttctta aaactagtat
    33841 atacaaagtt aaaaaccttt aaaactaaag ttttgtcaca gattttgtgt caaaacatta
    33901 cttttgacga ggctgggcaa aaaggcttaa aaattataaa acgcatcata tcaggtgtac
    33961 atgaactac
//
LOCUS       DBERMR010000020        33675 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.020, whole genome shotgun sequence.
ACCESSION   DBERMR010000020 DBERMR010000000
VERSION     DBERMR010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 33675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33675
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.020"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            467..1630
                     /locus_tag="ACL8A1_001014"
     CDS             467..1630
                     /locus_tag="ACL8A1_001014"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HGS1201803.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQTFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGFDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            1627..2304
                     /gene="aroD"
                     /locus_tag="ACL8A1_001015"
     CDS             1627..2304
                     /gene="aroD"
                     /locus_tag="ACL8A1_001015"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HGS1201804.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQFYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            2294..3148
                     /locus_tag="ACL8A1_001016"
     CDS             2294..3148
                     /locus_tag="ACL8A1_001016"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HGS1201805.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLVETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            3167..4234
                     /gene="aroB"
                     /locus_tag="ACL8A1_001017"
     CDS             3167..4234
                     /gene="aroB"
                     /locus_tag="ACL8A1_001017"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HGS1201806.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     IAEEKGLMPAGITQSITEMCQKFGLPVDYENWKVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            4244..5410
                     /gene="aroC"
                     /locus_tag="ACL8A1_001018"
     CDS             4244..5410
                     /gene="aroC"
                     /locus_tag="ACL8A1_001018"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HGS1201807.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            5420..6523
                     /locus_tag="ACL8A1_001019"
     CDS             5420..6523
                     /locus_tag="ACL8A1_001019"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HGS1201808.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEVIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            6534..6872
                     /locus_tag="ACL8A1_001020"
     CDS             6534..6872
                     /locus_tag="ACL8A1_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HGS1201809.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            6964..8247
                     /gene="aroA"
                     /locus_tag="ACL8A1_001021"
     CDS             6964..8247
                     /gene="aroA"
                     /locus_tag="ACL8A1_001021"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HGS1201810.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKECTRNHTEDMLKQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIVPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEICGALIPRLIDELPIIALLATQAQDVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVAGGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            8240..8716
                     /locus_tag="ACL8A1_001022"
     CDS             8240..8716
                     /locus_tag="ACL8A1_001022"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HGS1201811.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     QRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            8713..9561
                     /gene="pheA"
                     /locus_tag="ACL8A1_001023"
     CDS             8713..9561
                     /gene="pheA"
                     /locus_tag="ACL8A1_001023"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HGS1201812.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAIAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWLLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            9558..10832
                     /gene="psr"
                     /locus_tag="ACL8A1_001024"
     CDS             9558..10832
                     /gene="psr"
                     /locus_tag="ACL8A1_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HGS1201813.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFVYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            11111..11956
                     /locus_tag="ACL8A1_001025"
     CDS             11111..11956
                     /locus_tag="ACL8A1_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HGS1201814.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKNFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            11946..13049
                     /locus_tag="ACL8A1_001026"
     CDS             11946..13049
                     /locus_tag="ACL8A1_001026"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGS1201815.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLTRRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYASKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYEIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFNVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            13051..14037
                     /locus_tag="ACL8A1_001027"
     CDS             13051..14037
                     /locus_tag="ACL8A1_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGS1201816.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAKKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGYAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            14053..15486
                     /locus_tag="ACL8A1_001028"
     CDS             14053..15486
                     /locus_tag="ACL8A1_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HGS1201817.1"
                     /translation="MIDGKRLLFSLTIVSYTLTIVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGVLARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            15515..16549
                     /locus_tag="ACL8A1_001029"
     CDS             15515..16549
                     /locus_tag="ACL8A1_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HGS1201818.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPEDSAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            16749..17486
                     /gene="mecA"
                     /locus_tag="ACL8A1_001030"
     CDS             16749..17486
                     /gene="mecA"
                     /locus_tag="ACL8A1_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HGS1201819.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            17637..18923
                     /locus_tag="ACL8A1_001031"
     CDS             17637..18923
                     /locus_tag="ACL8A1_001031"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HGS1201820.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLVAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILAPDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            18925..19794
                     /gene="thrB"
                     /locus_tag="ACL8A1_001032"
     CDS             18925..19794
                     /gene="thrB"
                     /locus_tag="ACL8A1_001032"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HGS1201821.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            20044..20982
                     /gene="msrB"
                     /locus_tag="ACL8A1_001033"
     CDS             20044..20982
                     /gene="msrB"
                     /locus_tag="ACL8A1_001033"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HGS1201822.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKTSLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            21040..22764
                     /locus_tag="ACL8A1_001034"
     CDS             21040..22764
                     /locus_tag="ACL8A1_001034"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1201823.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISTELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSGRVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVKLFKGTIRSNLTLGLNQEVSDQKLWQALEIAQAK
                     DFVSEKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFIILDDATSALDTITESK
                     LLKAIRENFPNTSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            22766..24514
                     /locus_tag="ACL8A1_001035"
     CDS             22766..24514
                     /locus_tag="ACL8A1_001035"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1201824.1"
                     /translation="MKRQTVNQTLKRLAVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFVDRQGSGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKIAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRASLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQFPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            24771..26030
                     /locus_tag="ACL8A1_001036"
     CDS             24771..26030
                     /locus_tag="ACL8A1_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HGS1201825.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIELLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVVVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    26106..26241
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            26280..26780
                     /gene="rplJ"
                     /locus_tag="ACL8A1_001037"
     CDS             26280..26780
                     /gene="rplJ"
                     /locus_tag="ACL8A1_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HGS1201826.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            26856..27224
                     /gene="rplL"
                     /locus_tag="ACL8A1_001038"
     CDS             26856..27224
                     /gene="rplL"
                     /locus_tag="ACL8A1_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HGS1201827.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            27411..27581
                     /locus_tag="ACL8A1_001039"
     CDS             27411..27581
                     /locus_tag="ACL8A1_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201828.1"
                     /translation="MQKEGQSSRQFMKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQ"
     gene            27581..27829
                     /locus_tag="ACL8A1_001040"
                     /pseudo
     CDS             27581..27829
                     /locus_tag="ACL8A1_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            27843..28166
                     /locus_tag="ACL8A1_001041"
     CDS             27843..28166
                     /locus_tag="ACL8A1_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201829.1"
                     /translation="MTFLDDYHKKHNYPLFYESYLQNVMEFLESQDIKNGVDAFVDDH
                     QNLVFVLYGQGYRAEGKEGILTTQVTVKAYDEDKKPINFANLLDSLIVSEYQMEPNLW
                     EVSYD"
     gene            28159..28539
                     /locus_tag="ACL8A1_001042"
     CDS             28159..28539
                     /locus_tag="ACL8A1_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201830.1"
                     /translation="MIDLYLSKNSRRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLKLTDFIEMILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            28552..28785
                     /locus_tag="ACL8A1_001043"
     CDS             28552..28785
                     /locus_tag="ACL8A1_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGS1201831.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRHYKMEQRMPQSPWVERIVQVLDLDYEDLFRR"
     gene            28788..29375
                     /locus_tag="ACL8A1_001044"
     CDS             28788..29375
                     /locus_tag="ACL8A1_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HGS1201832.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYCASLIS
                     FLWMYAQRKQAHKVNMKLRIKWIGIGLASLLIISLCFSLIHAQESTNQANLIGLQYQV
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHPGTIIAWCLYV
                     SLRMFLGLVRYKLDLWGSMGLHLVWNLLVYSLLLF"
     gene            29577..29714
                     /locus_tag="ACL8A1_001045"
                     /pseudo
     CDS             29577..29714
                     /locus_tag="ACL8A1_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
     gene            30004..31407
                     /locus_tag="ACL8A1_001046"
     CDS             30004..31407
                     /locus_tag="ACL8A1_001046"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201833.1"
                     /translation="MNNDEWVYQYPIGKFVERQGWKIHISSEYNSSHELLQDVAKICH
                     EMRIPFKHLSTEDKFIMRNGKLVSRGFSGKFITCYPNQNELESVLQKLESALKQYNGP
                     YILSDKRWDEVPIYLRYGVFRPSRDDEKKVAIDELIVGDEVVKDERLPVFKIPKGIVL
                     PDFLNKWLDKKDKKQGDFPFIIDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLG
                     FDGTYSSERLASECKALKILNEWSETPKIYWCGKIWEHTFLGIEHMKGVPLNRWVTNN
                     FPLYEVVDKTKDYLLRVSKIVEKLIDLTNKFHSENVYHQNLHLGNILVKDEDEISIID
                     WEQAVFSNDEKVVHKVAAPGFRAWRETLPSEIDWYGIRQIAHYLYMPLVTTSDLTYNY
                     VSQTRIEGKKLFESLGYTREHIDYVESLLSYLDSKCPQIENISRKKVLKPMHEIRTIE
                     SEQDIQDFIIKAFLLVN"
     gene            31596..32216
                     /locus_tag="ACL8A1_001047"
     CDS             31596..32216
                     /locus_tag="ACL8A1_001047"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS1201834.1"
                     /translation="MKDVVYSITPENKLSIDFSFKSPFRVLLTGTSGSGKTTILNLIN
                     GSLKPQKGYVNLLSHGKKSSDSIPTVDQTPYIFDTTIRENVTLFQNEYFSDDQIIEVL
                     KKVNLYEELEKIDILNYQCGENGSNLSGGQKQKIALARALIRNNKVYLFDEISANLDN
                     DNSNSIHDILFNLGISFIEVSHHYDLNDKRYTDIYKLENGTLFKIK"
     gene            32231..32971
                     /locus_tag="ACL8A1_001048"
     CDS             32231..32971
                     /locus_tag="ACL8A1_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1201835.1"
                     /translation="MDKDYILKVKGLYHQFLLGNNKTLQVLKNVSLSASRGEFISILG
                     ISGSGKSTLLKCISSLLEPTSGEVILNGINPYKIRNAKLSSIRRNEVSFIFQAYNLIP
                     SLPVIENIALPLRLSQKKLTIKNVENLLKRMKFNAGLNDFVGTLSGGEQQKVAIARAV
                     IADSDIIFADEPTGALDSVSREVIFELLRELVGAGKCVIMVTHDIELASKTDRALILK
                     DGKIFKELHRPSGEELYKILEVQSTTEE"
     gene            32974..>33675
                     /locus_tag="ACL8A1_001049"
     CDS             32974..>33675
                     /locus_tag="ACL8A1_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201836.1"
                     /translation="MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASLLQLSF
                     LVGGQIFIISSIISFIAYLCSIFVTSNYYYFLQYFFGENILPDIQFQTSAVGCIITVV
                     LISFLAFLSGCFYTFKKIRNRKSSKIRHVLSIVKRILLLAGFSVIWLLSLQQIFQDST
                     ILAKAQIIFNIVILDI"
BASE COUNT        10257 a         5935 c         7399 g        10084 t
ORIGIN      
        1 atttttgata gaacatatat ttagagttcg aaatagaatg atgcagatag gtgaaattat
       61 aattattgtt gcttctataa tattatttat tgatagtata ttgagtttgt aacaaaaggc
      121 ttctctataa aatcaaattt tccagatacc tattgaagca gatttatttc ttcatctttc
      181 ttctactacc tttgtcacta aatttgacag acacatattg ctgattcaac ctctctacct
      241 ataatctcac agttaacctg aggctagttt catagtttgt tatttgattt tcgttgataa
      301 aaagaaatgc tcctccaaga cggaagagca ttttttattg tgattcttac acatttactt
      361 cctagttttg caggaagtct agtataccat tttttaaaag aagagtaaaa aagcaagggt
      421 agtttctttt ttgagaattg tctaaaagtt tgatataatg gtagttatga atagaataag
      481 agtcagcaaa agggttgaaa agaagcttgc taaggggcta gttttactag aagccagtga
      541 tcttgagaat gtcaatctta aggatcagga agtagaggtg cagggtcagg aaggaaactt
      601 tcttgggact gcctaccttt ctcagcaaaa caagggcttg ggctggtttg tcagcaaaga
      661 caaggtggcc ttcaatcaaa ctttctttga aacgttgttt agaaaagcca aagaaaagag
      721 aaacgcctac tatcaagatg atttgacaac tgcctttcgt ctctttaatc aagagggaga
      781 tggctttggg ggtctgacag tggaccttta tggcgactac gccgtctttt cttggtataa
      841 ctcttatgtt tatcagattc gtcagactat atcagaagcc tttagacagg ttttccctga
      901 ggttttaggg gcttatgaga aaatccgctt taagggtttt gactatgaat ctgcccatgt
      961 ttatggtcaa gaagcacctg actttttcac tgttttagaa aatggtgtcc tgtatcaagt
     1021 ctttatgaat gatggcttga tgacaggaat tttcctagac cagcatgagg ttcgcggtag
     1081 tttagttgac ggcttggcta tgggtaaatc cttgctcaat atgttttcct acacagcggc
     1141 tttttcagta gctgcggcca tgggaggagc tagccagaca acttctgttg atctagccaa
     1201 acgttcacga gaattgtctc aagcgcattt tcaggcaaat gggctcagca cagacgagca
     1261 tcgttttata gtcatggatg tctttgagta tttcaaatat gccaaacgca aagacttgac
     1321 ctacgatgtt attgtcctag atccgcctag ctttgctcgg aataaaaaac aaactttctc
     1381 tgtggccaag gattatcaca agttgatttc ccagagtctt gagattttaa atccgggagg
     1441 gattatcatt gccagtacca atgctgccaa tgtttcccgt cagaaattta cagaacaaat
     1501 tgataaaggt tttgcaggaa gaagttacca gattttaaac aaatatggtc ttccagcaga
     1561 ttttgcctat aataaaaaag atgaaagtag taattacctc aaggtgatta gtatgaaggt
     1621 tagtaaatga aattaatcgt ttcagtaatg ccaagaagtt tagaggaggc tcaggctctg
     1681 gatgccacga ggtacctgga tgccgacatc attgaatggc gtgccgacta tctgcctaaa
     1741 gaagcgattt tgcaggtagc tccagccatt tttgaaaaat tcgcaggccg tgagttggtt
     1801 ttcacgctac gaactcgctc cgaaggtgga gaaatcgacc tttctccaga agagtatatc
     1861 catctaatca aggaagttgc gcaattctat caaccagact atattgattt tgagtactat
     1921 agctacaagg atgtttttga ggaaatgctg gacttcccaa atcttgtttt gagttaccat
     1981 aatttccaag aaacacctga gaacatgatg gaaatcttgt cagagttgac gatcctaaat
     2041 ccaaaacttg ttaaggttgc ggtgatggct cacacggagc aggatgtctt agacttgatg
     2101 aactatacac gaggctttaa aaccctcaat cctgaacagg aatatgtgac catttctatg
     2161 ggtaaggtgg gcaaggtctc tcgtatcact gcggatgtga ctggttcgag ttggtctttt
     2221 gccagtctgg atgaggttag tgcccctggg cagatttctc tagctagcat gaaaaaaatc
     2281 agggagattt tggatgaagc ttgatggcta tacacgttta gctgcagttg ttgccaatcc
     2341 tattaagcat tctatttctc ccttcatcca caatagagcc tttgaggcga cagctaccaa
     2401 cggtgcttat gtggcttggg agattgaagc gagtgacttg gtagaaacag tggccaatat
     2461 tcgtcgctac cagatgtttg gcatcaatct gtccatgccc tataaggagc aggtgattcc
     2521 ttatttggat aagctgagcg atgaagcgcg cttgattggt gcggttaata cggttgtcaa
     2581 tgagaatggc aatttaattg gatataatac agatggcaag ggatttttta agtgcttgcc
     2641 ttcttttaca atttcaggta aaaagatgac cctgctgggt gcaggtggtg cggctaaatc
     2701 aatcttggca caggctattt tggatggcgt cagtcagatt tcggtctttg ttcgttccgt
     2761 ttctatggaa aaaacaagac cttacctaga caagttacag gagcagacag gctttaaagt
     2821 ggatttgtgt gctttagaat atgtttctga actgcaagca aggattgccg agtcggattt
     2881 gctagttaat gccaccagtg tgggcatgga tggccaatcc tctcctgttc ctgaaaacat
     2941 agtcttacca gaaactcttt tagttgcaga tatcatatac caaccctttg aaacaccgtt
     3001 tttgaaatgg gctagaagtc agggcaatcc agcagtcaat ggtctgggaa tgttgctcta
     3061 tcaagctgca gaagcttttc aactgtggac aggcaaggaa atgccgacag aagagatttg
     3121 gcagtcttta acagaaaaat accaataaag aaagggagac tctcctatga aaatcagaat
     3181 cgatattcct catcatcctt atgatattca gattgaaaaa ggttgtatgg cccaggctgg
     3241 tcagtggttg cgagaactct ggcaaccgca aaaggtagtc attgtgacag ataaccatgt
     3301 agcctctctc tatgcagaga aggtcaagct cagcctagaa gatgctggtt ttcaggtagc
     3361 tgtttttgat ttcttagaag gtgaagaaag aaagaattta actactgttc agaaagtcta
     3421 tgaatttcta gtcaagcaag gtctgactcg tagcgatgga atcgttgctc ttggtggtgg
     3481 cgttgttggg gacctggctg gctttgtagc ctctacctat atgcggggta ttcactttgt
     3541 tcagattccg actagtttga cagctcaggt tgattcttct atcggtggaa agacaggcgt
     3601 taacacacca tttgctaaaa atatggtggg gacctttgcc caaccagatg gggttctgat
     3661 tgatccactt gttcttgaaa ccctcggaaa aagagagttg attgaaggga tgggtgaagt
     3721 tatcaagtat ggcttgattg aggatccaga actgtgggct ctcttgacgg gactgaatgg
     3781 ttctgttgag agtattttgg aacatgcaga gaccttgatt gaacattctt gtcaggtgaa
     3841 gcgcaagatg gtggttgaag atgagttgga caatggtatt cgtctttacc tcaattttgg
     3901 ccacactatt ggccatgcca tcgaagcgac tgccggttat ggcaaggtca tgcatggaga
     3961 ggctgttgcc atgggaatgg tacagatttc caagattgct gaggaaaaag gcctcatgcc
     4021 agctggcatt acccaatcta tcacagagat gtgtcagaaa ttcggcttgc ctgttgacta
     4081 tgaaaattgg aaagttgaca agctttatca ggctcttact catgacaaga aagcgcgtgg
     4141 taacaccttg aaattggtct tggtgccaga gcttggttca gcgaccattc acccagtttc
     4201 tctggaagag atgaaagact acttggtaaa ataaggagag tgtatgagat atttaactgc
     4261 aggagaatca cacggccccc gtctaacagc tattattgag ggaattccag ctggacttcc
     4321 attgacagct gaggatatca atgaggatct tagacgccgt cagggtggct acggtcgtgg
     4381 tggtcgtatg aagattgaga gtgaccaggt tgtctttact tcgggtgttc gccacgggaa
     4441 gacgacaggg gcgcctatta ctatggatgt catcaataag gaccaccaga aatggctgga
     4501 catcatgtct gcggaggaca ttgaagaccg ccttaaaagc aagcggaaaa ttactcatcc
     4561 tcgcccaggt catgccgatt tggttggggg gattaagtac cgttttgatg atttgcgaaa
     4621 ttctttggag cgttcatcag ctcgtgaaac caccatgcgg gtggcagttg gtgcagtagc
     4681 caaacgcctc ttggctgagc tggatatgga gattgccaac catgtcgtgg tctttggtgg
     4741 taaggaaata gatgttcctg aaaatctgac agtcgctgaa attaagcaaa gagctgccca
     4801 gtctgaagtt tctattgtca accaagaacg agaacaggaa atcaaggact atattgacca
     4861 aatcaaacgt gatggtgata ccatcggtgg ggttgtggag acagtcgtcg gaggagttcc
     4921 agttggtctt ggttcctatg tccaatggga tagaaaattg gatgcaagat tggctcaagc
     4981 tgttgtctct atcaatgcct ttaaaggggt ggaatttggt cttggctttg aggctggtta
     5041 tcgtaaaggc agccaagtta tggatgaaat tctctggtct aaagaagacg gttatactcg
     5101 ccgtaccaat aatctaggtg gttttgaagg tggtatgact aatgggcaac ccatcgttgt
     5161 tcgtggggtc atgaaaccca ttcctactct ttataaacct cttatgagtg tggatatcga
     5221 aacccacgaa ccttacaagg caaccgtgga gagaagtgat ccgactgctc ttccagctgc
     5281 aggaatggtc atggaagcag ttgtagcaac ggttctggcg caagaaatcc tcgaaaaatt
     5341 ctcatcagat aatcttgagg aactaaaaga agcggtagcc aaacaccgag actatacaaa
     5401 gaactattaa ggagttccta tggcaaaaac aatctatatc gcaggtcttg ggttgattgg
     5461 agcctctatg gcacttggta tcaaacgcga tcatccagat tatgaaattt taggttataa
     5521 tcgtagtcaa gcttcgagag atatcgcctt gaaagaaggt atgattgacc gtgcaacgga
     5581 tgattttgct agttttgctc ctttggcaga tgtcattatc ctcagcttgc caatcaaaca
     5641 aactattgct ttcattaagg agttggccaa tttggatttg cgagaaggcg ttattatttc
     5701 agatgctggt tcgaccaagt caaccattgt ggatgcggcg gagcagtatt tggctggcaa
     5761 gtctgttcgc tttgtcgggg cccatcccat ggctggtagt cacaagacag gggctgcttc
     5821 ggcagatgtc aatctttttg aaaatgccta ttatatcttt acaccttcaa gcctgacaag
     5881 tcaggacacg cttaaggaaa tgaaggatct gctttcaggt cttcatgctc gttttatcga
     5941 gattgatgcc aaggagcatg atcgtgtcac ttctcagatt agccattttc ctcatatttt
     6001 ggcttctagt ctcatggagc agactgcggt ctatgctcaa gagcatgaga tggcaaggcg
     6061 ctttgcggca ggtggttttc gagatatgac ccgaattgcg gaaagcgagc caggaatgtg
     6121 gacctccatt ctcttgtcca atagcgagac cattctggat agaattgagg atttcaagga
     6181 acgtttggaa gtgattggtc aggccattag taagggagat gaagagcaaa tttggaactt
     6241 ttttaaccaa gcgcgtgaac aacgccagac catggaaatc cataagcgtg gtggtgtgga
     6301 tagctcttac gacctctatg ttgacgttcc tgatgaagaa gatgtcatcc tgcggatttt
     6361 ggaactgcta cgtggaactt ccttggttaa catccacatc aacgaggaaa atcgtgagga
     6421 tattcacgga atcctacaaa tttcatttaa aaatgctcaa gacttggaaa gagctgagca
     6481 tctcataaca gaaaataccg actatacagt cgtcatcaaa taaggagaag aacatgtcaa
     6541 atatttacga tagtgcaaac gaactcagtc gcggtctacg cggattacca gaatacaagg
     6601 ctgttaaagc agctaaagat gcgattgcag cagatgctga ggcaagcaaa atctttacag
     6661 aatatcttgc ctttcaagag gaaattcaaa aactagcaca gacaggtcaa atgccagatg
     6721 cgtccttcca agctaagatg gaaggctttg gtaaacagat tcaaggaaat agcctcttgt
     6781 cagaattctt taccaagcaa caacaattgg ccatttacct ttctgacatt gaaaaaattg
     6841 tttttgaacc agtttcagaa ttgctgaaat aagaaaagaa cgatcctaaa gtcaggaatt
     6901 tttaaggaat ttctgacttt ttatgataaa ataggtaaaa gtattgcaag tatgaggtcc
     6961 agtatgaaac taaaaacaaa cattcgccat ttacatggta gtatccgcgt cccaggtgac
     7021 aagtctatca gccaccgttc cattatcttt ggaagtttgg ctgagggtga gaccaaggtt
     7081 tatgatattc tgcgaggtga agacgttctt tcgaccatgc aggtttttcg tgaccttggt
     7141 gttgaaattg aggataaaga tggggttatt accattcaag gtgtaggcat ggctggctta
     7201 aaagcgccac aaaatgccct taatatggga aattctggca cctcgattcg cctgatttca
     7261 ggtgtccttg ctggtgcaga tttcgaagta gagatgtttg gagatgatag tctttccaaa
     7321 cgtcctatgg accgtgtgac ccttccactg aaaaaaatgg gcgtcagcat ttcagggcaa
     7381 actgaacgag acttgcctcc ccttcgctta aaagggacga aaaacctaag acctattcat
     7441 tatgagttgc caattgcctc tgcccaagtc aagtcagcct tgatgtttgc agccttacaa
     7501 gctaaggggg agtcagttat tatcgaaaaa gagtgcaccc gtaatcatac tgaagatatg
     7561 ttgaaacaat ttggtggtca tttaagtgtg gacggtaaga aaatcacagt ccaagggcca
     7621 caaaaattga caggacagaa ggtggtcgta ccaggagata tttccagtgc agccttttgg
     7681 ttagtcgcag gtttgattgt tccaaattct cgtctagtgc tgcagaatgt ggggatcaat
     7741 gaaactcgca ccggtattat tgatgtcatt cgtgccatgg gtggaaaatt ggaaataact
     7801 gaaatcgatc cagtcgctaa atctgcaacc ttgattgttg agtcttctga cttgaaagga
     7861 acagagattt gtggcgcttt gattccacgt ttgattgatg aattgcctat tattgcccta
     7921 cttgcgaccc aagcccaaga tgtaacagtt atcaaggatg ctgaggagct caaggtcaag
     7981 gaaacagacc gtattcaggt tgtggcagac gccttaaata gtatgggagc agatattact
     8041 cctacggcag atgggatgat tatcaaagga aaatcagctc ttcacggtgc tagagtcaat
     8101 acgtttggtg accaccgtat cggcatgatg acagctatcg cagccctatt ggttgcaggt
     8161 ggagaggtgg agcttgaccg tgcagaagcc atcaatacca gctatcctag tttctttgat
     8221 gatttggaga gcttgattca tggctaaggt gttattaggg tttatggggg ctggaaaatc
     8281 gactattgca agaggcttgg accctaatta ccttgatatg gatgctctga ttgagaagcg
     8341 cctaggtatg tccattgcga attttttcgc tgaaaaggga gaagcagcct ttcgtcaggt
     8401 agaatcagaa gtcctagctg atttactaca aacagaccaa gtcgtgtcaa ctggaggagg
     8461 agtggttatt tctcagagaa atcgtgactt actcaagact aatacagata acatctacct
     8521 gaaagcagat tttgaaaccc tctaccaacg tatcgcagct gataaggaca atcagcgacc
     8581 gctttttcta aataatagca aggaagaact agtagctatt tttcaagaaa gacaggcttg
     8641 gtatgaggaa gtggctagtc gggttttgga tgtgaccaag ctaagcccag aggaaattat
     8701 agaggaacta agatgaaaat tgcttatcta ggtcccaaag gatcattttc acaccacgtt
     8761 gtgcagacag cttttcctca tgaggaattg caggcttttg ccaacattac agatgtcatc
     8821 aaggcttatg agcaaggatt agtggactat tctgtggtgc cagttgaaaa ttctattgag
     8881 ggtagtgttc atgaaacctt ggactatctt tttcatcagg ctcacatcca agcagtagca
     8941 gaaatcgttc agcctattca tcagcagttg atggtggttc caggccatac taagattgaa
     9001 aagatttttt cacatccaca ggccttggct caaggaaaga aattcatcga tgaacaatat
     9061 ccagaggctc aaatcgaggt aacagctagt acagcttatg cggcccgttt tatttccgaa
     9121 catccagatc agccctttgc agcaattgca cctagaagtt ctgctgaaga atatggattg
     9181 gaactgattg ccgaggatat tcaggaaatg gaagccaatt tcacacgttt ctggcttcta
     9241 ggagctgaaa agcctagtat tcccttgcaa gcacaaactg aaaagatgag tttggccttg
     9301 acattacctg acaaccttcc aggtgcactt tataaggccc tgtcgacctt tgcttggcga
     9361 ggaattgact tgacaaaaat tgaaagtcgt ccactcaaga cagcactggg tgaatacttt
     9421 ttcattatcg atgtggatta taccgataag gacttggtcc actttgccca aaaagaatta
     9481 gaagcgattg gaatccagta taaaattctg ggtgcctatc ctatttatcc aatatcagac
     9541 catggaaagg agagaagatg agtaaagaaa atcccttaag tcatcatgag cagttgcgtt
     9601 atgattattt gctaaaaaat attcactatc tcaatgagag agaaaaaaat gagtttgtct
     9661 atttgcaaga aaagctaact cttgctaggg gaaatagtag ctctagcttg gaacaagaaa
     9721 gagaagagca ggttgactta ccaagctatg cgaaccggag tcgctcacaa tccaaatcac
     9781 aagcactctc tttccctcca aaaaagaaaa gacggaagct ccgtcttaaa cgaattttta
     9841 tggtgatttt ttcacttttg gtctgtgtgg ctttggccat ggtattcatg tttttgcgtg
     9901 gttaccaaga tgctagcgca aagaaaactg ctgatgcccg ggcagctcaa gtagaagtct
     9961 ttaatggtca ggacactaga gatggagtta atattttaat catgggtact gatggtcgaa
    10021 tcggccagaa cagtgttgag acacgaactg actctattat ggtattaaat gtcgggggct
    10081 cagataagaa aatgaagcta gtcagtttca tgcgtgataa tttggtctat atagatggtt
    10141 atagtcaagt gattaacggt agaaaacaga cagataacaa gttaaacgta gcctacgagt
    10201 taggagaaca agaggggcaa aaaggggcag aaatggttcg ccaagtcttg aaagataatt
    10261 ttgacttgga cattaagtac tacgccttgg ttgatttcca ggcctttgcc acagcgattg
    10321 acacgctttt ccctgatggg gtgacgattg atgctcaatt ttcaacattg aatgggcgtc
    10381 ccctaacaga agctacagtc ggagatgatt tatacgctac cgagactgag tctccaaccc
    10441 aaaccattaa agtcggaaaa cagcagatga atggctcgac cctgctcaat tatgctcgtt
    10501 tccgtgatga tgatgaggcg gattacggcc gtacaaaaag acagcaacaa gttttaacag
    10561 caattttaga gcaaattaaa gatcccacta aacttttcac tggttctgaa gctcttggaa
    10621 aggtttttgc tatgacctca accaacgtac cctatacttt cctcctaaca aacggtttat
    10681 ctgttttgga tggagcaaaa aatggtattg aaaaattgac gattccagaa ctgggtgact
    10741 gggtagatgc ctatgatgtt tatggaggct tgggcttgct ggttgatcaa aacaaatatc
    10801 aaaccaagct cgctcaaatg ggcttgagat aagataatac tcaatgaaaa tcaaagtgca
    10861 aattaggaag ctagccgcag gctgctaaaa acacagtttt gaggttgtag ataagactga
    10921 cgaagtcagt tacctatacc tacggcaagg tgaagctgac gttgtttgaa gagattttcg
    10981 aagagtataa gacagaaaaa tcaaagtttg atagctattt ggatagcagt caggctttga
    11041 ttttttacag tctgcaatag attttcaccc cttgtttgtg gtataatgaa acgatacgat
    11101 agaaagaata atgaataata tgactgattt aaaagcaatt caggctcgta gtctggagat
    11161 ggctgaatat tttgtggcct tttgtaaaga acatgatttg ctctgttatc tctgtggcgg
    11221 aggcgctatt ggtgcccttc gaaacaaggg atttattcct tgggacgacg acctagactt
    11281 ttttatgcct cgtaaagatt atgagaaatt agcagaatta tggcctcgtt atgcagatga
    11341 acgttatttc ttgtcaaaga gtcacaagaa ttttgttgat cgtaatcttt ttattaccat
    11401 tcgtgacaag gaaaccacct gtatcaagcc ttatcagcag gatttggatt tgccacatgg
    11461 tctggccttg gatgttttgc ctttggatta ttatccgaaa aatccagctg agcggaaaaa
    11521 acaggttcgt tgggccttga tttattcact cttttgtgcg caaactattc cagaaaagca
    11581 tggtgctctc atgaaatggg gaagtcgcat tttactgggt ttgactccaa aatctctccg
    11641 ttatcgcatc tggaaaaaag ctgagaaaga aatgactaag tatgatttgg ctgattgtga
    11701 tggcattaca gaattatgct caggtcctgg ctacatgaga aacaagtacc caatcacatc
    11761 ttttgaagac aatcttttct tgccatttga aggaacagag atgcctattc caatcggcta
    11821 tgatgtctat ctcagaactg cttttgggga ttatatgacg cctccaccag cagacaagca
    11881 ggtaccgcat catgatgctg tcatcgctga tatggataag tcttatacag aatacaaggg
    11941 agaatatggt ggctaagaaa aaaatcttat tttttatgtg gtctttttct cttggaggtg
    12001 gtgcagagaa gattctatca accattgttt caaatctgga tccagaaaag tatgatattg
    12061 atattcttga aatggagcac tttgacaagg gatatgaatc tgttccaaag catgtacgca
    12121 ttttaaaatc ccttcaagat tatcgccaaa ccagatggtt acgagctttt ttgtggagaa
    12181 tgagaattta ttttccaaga ctgactcgtc gtttgcttgt aaaagatgat tatgatgttg
    12241 aagtttcttt taccattatg aatccaccac tgttgttctc taaaagaaga gaagtcaaga
    12301 agatatcttg gattcatgga agtattgaag aacttcttaa ggatagctct aaaagagaat
    12361 cacatagaag ccagttggat gctgcgaata caattgtagg gatttcaaaa aagaccagca
    12421 attctatcaa ggaagtttat ccagattatg cttctaaatt acagacaatc tacaatggat
    12481 atgattttca gactattcta gaaaaatctc aagagaagat cgatatcgag attgctcctc
    12541 aaagtatctg tactatcgga cggattgagg aaaataaggg ttctgaccgt gtagtggaag
    12601 tgatacgatt attacaccaa gagggaaaaa actatcatct ctattttatc ggggctggtg
    12661 atatggaaga ggaactgaaa aaacgagtca aagagtatga gattgaggac tatgtacatt
    12721 tccttggtta tcaaaaaaat ccttatcagt atttatctca gacgaaagtt ctcttgtcta
    12781 tgtctaaaca agaaggcttt cctggagtgt atgtggaggc cttgagtctg ggactccctt
    12841 ttatctctac ggacgttgga ggggctgagg aattatccca agaaggacga tttggacaaa
    12901 tcattgagag caatcaagag gcagctcagg cgattactaa ttacatgact tctgcctcaa
    12961 actttaatgt cgatgaggct agccaattca ttcaacaatt tacaattaca aaacaaatcg
    13021 aacaagtaga aaaactatta gaggagtagc atggaaactg cattaattag tgtgattgtg
    13081 ccagtctata atgtggcgca gtacctagaa aaatcgatag cttccattca gaagcagacc
    13141 tatcaaaatc tggaaattat tcttgttgat gatggtgcaa cagatgaaag tggtcgcttg
    13201 tgtgattcaa tcgctgaaca agatgacagg gtgtcagtgc ttcataaaaa gaacgaagga
    13261 ttgtcgcaag cacgaaatga tgggatgaag caggctcacg gggattatct gatttttatt
    13321 gactcagatg attatatcca tccagaaatg attcagagct tatatgagca attagttcaa
    13381 gaagatgcgg atgtttcaag ctgtggtgtc atgaatgtct atgctaatga tgaaagccca
    13441 cagtcagcca atcaggatga ctattttgtc tgtgattctc aaacatttct aaaggaatac
    13501 ctcataggtg aaaaaatacc tgggacgatt tgcaataagc taatcaagag acaaattgca
    13561 actgccctat cctttcctaa ggggttgatt tacgaagatg cctattacca ttttgattta
    13621 atcaagttgg ccaagaagta tgtggttaat actaaaccct attattacta tttccataga
    13681 ggggatagta ttacgaccaa accctatgca aagaaggatt tagcctatat tgatatctac
    13741 caaaagtttt ataatgaagt tgtgaaaaac tatcctgact tgaaagaggt cgcttttttc
    13801 agattggcct atgctcactt ctttattctg gataagatgt tgctagatga tcagtataaa
    13861 cagtttgaag cctattctca gattcatcgt tttttaaaag gctatgcttt tgctatttct
    13921 aggaatccaa ttttccgtaa ggggagaaga attagtgctt tggccctatt cataaatatt
    13981 tccttatacc gattcttatt actgaaaaat attgaaaaat ctaaaaaatt acattagaac
    14041 gggggtgacc aagtgattga tgggaaacga ttattattta gtttgaccat agtcagttat
    14101 accttgacga tagtaagtgg aattgtgtat ctgtttaata ataataatgt tagcttactt
    14161 tctactttat tgttcttgtt ggttagtagc ttaattgctt gttggaatga tatcaagtat
    14221 tacttaatcc attttatttt ctatttaacc atttttgtat ttctggtatc aagaccgacc
    14281 attgattatt ttagggatgg tgctttggat acctatcatc caatagccta tcgttttgcc
    14341 tttatagttg ttatggtttc gattctgggc ttgaccacag gaggcgtcct ggctcgttac
    14401 ttcatagcta ggaagaaaat aaaagtagca aatataggaa attctctaaa agaggtttat
    14461 atcaagcggt tacgctttgt atcactagga gtttttcttc taacctatcc tttctatttc
    14521 attcggttat ttgaacggct cttgtatcgt ttgcagactt cctactatgc ctactatgca
    14581 aattttgaaa gtaaactgcc ttattttacc tacattttgt ctacctttac ggtctatgca
    14641 atgtgtatgt atctggcaac caagccaaag aaattgcagg ccacagcagt gcttgtctcc
    14701 tttattgcag ctaatactat tcatttggca attgggacac gaaatccctt tattttaagt
    14761 attttatttg cttttgttta ttactttatg cgggagcaaa ctgaaaaagg aaaatggatt
    14821 gggtttaaag aaaagttagc gatttttgta ggttctccta ttctcatgtt agcgatggga
    14881 gtacttaatt atgtacggga taatgtccaa gtttcccata caggtttctg ggatatctta
    14941 cttgacttta tctataaaca aggaactagt tttggtgttc tggctcgagg ttttctattt
    15001 aacagtagcc tcccttaccg agatttccgt aattttactt ttggtcctgt tcttgattat
    15061 tttgcaaggg ggagtttggg agccattttc ggaggaaaag cctttgaaca tacaaccaat
    15121 agtgtggaac tagctattga tagtaatagt tatgcccaca atctatccta tcttgtcttg
    15181 aacaaggaat acttgaaagg gcatggtatc ggaagtagtt atatcatgga gttgtatacc
    15241 gactatggta tgattggagt ctttctgctt agtttcttac tcggcgtatt atttatagcc
    15301 atgctgcaag tagcctatcg ctcaaggaca atcctatttg ctttatccct actcatcttg
    15361 aataatctat tctttatgcc aagaagcagc ttttcagaaa gtttcttcaa tttatttaca
    15421 atgcaattct ggggaattgt tcttgtgatt atatttgtag caaaaatgct tacaaaggaa
    15481 aactagtatc tactaaacaa aggagaaaaa aatcatgttt gaacattatt cagtagctga
    15541 tttgtttgca aatctttata aaaaacgaaa agcaaatatt ttagctctca tcgctttatt
    15601 tgctctcatt gctgtaccat ttacaattaa agcagttagg aataaaaaca ctgtcaaaga
    15661 tacaacaagt tattcaactt atcttatcta taaaatcact cctccagaag attcggccaa
    15721 aacgattttg aatcatcaaa ttggtggtta tagtgatttt tatgggaaat tgattgatgg
    15781 taatttgaat ggagcttatc ttttcaatga tgtagaaccc agtgagttga aaaaaattgc
    15841 cagtgaatta gacacgacgg aaacaacctt gaaaaattca acgaatgact attggtggaa
    15901 aaaattgacc gtctactata tgattgacga tgcaggggtt ggtgtgaaaa ttttgacatc
    15961 aagtaaagat gccaatgact tgttagagaa aaaaattgat gggttgattg agaaatttaa
    16021 acatgcttat gcaaatgtga aaattgaaaa actggaaacc atcaactcta aagaattgaa
    16081 cgcaaatggt gaaacagcgc ttggcttaaa tgtgaaaaat ctgattcttc gtttagttgt
    16141 tattggagtg gtttgtgtga ttttggttgt gatgggaaat gtgttagttt atctctttaa
    16201 tccaacaatc aatagagtag gtgatttttc tcagtatcaa attgattttg taacagagat
    16261 tacaacaatt gctaacctag cagatgtttt gtcatacaaa aatactggac aggaattgac
    16321 catcgttagc tcaaataaag ctatcctaga taaattgaaa cagagtcaag aagctttaaa
    16381 aggaatgcat tttgtagatt tgcaggatgt atcatctctt ttggaaagag atacagtcct
    16441 tcttgttgaa gagtacggag tgactcgtta taagaaattt gagcaaagtc ttcaaattct
    16501 cagaaactta aatcgttcta tcctcggtgt agcaaccttt aaattataag tattctgatg
    16561 tataaggtct tcaaaatcgg ctaagagccg attttgaagg ctttttctat ctgtctgcag
    16621 aaattttctc ttggagtctc ctatagaaag accctaggga aaatctatcc gtatagagga
    16681 gccttctttt gataaaatcg taaaaatcta gtataataga tagaactgaa agtatgaggt
    16741 tactagcaat gaaaatgaaa caaattagtg atacaacttt aaaaatcacg atgtctttag
    16801 aggatttgat ggatcgtgga atggagattg ctgactttct cgttcctcaa gaaaaaacag
    16861 aagagttctt ttatgctatc ttggatgagc tagagatgcc tgatagcttt ctggatacag
    16921 gcatgttgag cttccgtgtg actccaaaac ctgataaggt agatgtcttt gtgaccaagt
    16981 caaagattga ccaaaatcta gattttgaag acttatcgga tttgccagat atggaagaat
    17041 tggctcaaat gtctccagat gaatttatca aaaccctgga aaaaagcatc gcagacaaaa
    17101 ccaaggatga tatcgaagcc attcaatctc ttgagcaagt tgaagccaag gaagaagagc
    17161 aagagcaggc tgaacaagaa gctgagagta agaaagaacc ttacatctac tacatccttt
    17221 cttttgctaa gttggctgac ttggtagctt ttgccaagac agtgactttt gaaatggaaa
    17281 cttctgaact ctacaaaatg aacgagcgct attatttgac cattttagtg gatattgaaa
    17341 atcatccaag cccatatcca gcttggctgt tggcccgtat gcgcgagttt gcagacgata
    17401 gtgatatcag tcgctcagtc ttacaagagt atggtcaagt cttgatgagt cacgatgcag
    17461 tgctcaatct gcaaaaaatc ggctaatcct ttctggaaag ctattttcag attttaagaa
    17521 gagcgaatcg tctgattcgt tttttctttt tatactgaaa tagtgattta ctataatagg
    17581 aattttcaca aaattctgtt ataatggcta tattagaaaa tttcgaggag ataaagatga
    17641 cagttaaaat tgctttacta ggatttggta ccgttgcaag tggtgtgcct ttcctcctaa
    17701 aggaaaatgg aggaaaaatc aatcaatcag cacattcaga tatcaaagtt gctaaggtat
    17761 tggtcaagga tgaagatgaa aaaaatcgct tgcttgtagc agggaatgac tttaactttg
    17821 taaccaatgt ggatgatatt ttatcagacc aggatattac tatcgtagtg gaattgatgg
    17881 ggcgtattga gcctgctaaa acctttatca ctcgtgcctt ggaagctgga aaacacgttg
    17941 ttactgctaa caaggacctt ttagctgtcc atggcgcaga attgctagaa atcgctcaag
    18001 ctaacaaggt agcactttac tacgaagcag cagttgctgg tgggattcca attcttcgta
    18061 ctttagcaaa ttccttggct tctgataaaa ttacgcgcgt gcttggagta gtcaacggaa
    18121 cttccaactt catggtgacc aagatggtgg aagaaggctg gtcttacgat gatgctcttg
    18181 cggaagcaca acgtctagga tttgcagaaa gcgatccgac gaatgacgta gatgggattg
    18241 atgcagccta caagatggtt attttgagcc aatttgcctt tggcatgaag attgcctttg
    18301 atgatgtagc ccacaaggga atacgcaata tcacaccaga agacgtagct gtagctcaag
    18361 agcttggtta cgtagtgaaa ttggttggtt ctattgagga aacttcttca ggtattgctg
    18421 cagaagtgac tccaaccttc ctacctaaag cgcacccact tgctagtgtg aatggcgtaa
    18481 tgaacgctgt ctttgtagaa tctatcggta ttggtgagtc tatgtactac ggaccaggtg
    18541 cgggtcaaaa accaactgca acaagtgttg tagctgatat tgtccgtatc gttcgtcgtt
    18601 tgaatgatgg taccattggc aaagacttca acgaatatag ccgtgacttg gtcttggcaa
    18661 atcctgaaga tgtcaaagca aactactatt tctcaatctt ggctccagac tcaaaaggtc
    18721 aggtcttgaa gttggctgaa atcttcaatg ctcaagatat ttcctttaag caaatccttc
    18781 aagatggcaa agagggtgac aaggcgcgtg tcgttatcat cacacacaag attaataaag
    18841 cccagcttga aaatgtctca gctgaattga agaaggtttc agaattcgac ctcttgaata
    18901 ccttcaaggt gctaggagaa taagatgaag attattgtac ctgcaaccag tgccaatatc
    18961 gggccaggtt ttgactcggt cggtgtagct gtaaccaagt atcttcaaat tgaggtctgc
    19021 gaagaacgag atgagtggct gattgaacac cagattggca aatggattcc acatgatgag
    19081 cgtaatctct tgctcaaaat cgctttgcaa attgtaccag acttgcaacc aagacgcttg
    19141 aaaatgacca gtgatgtccc tttggcgcgc ggtttgggtt cttccagctc ggttatcgtt
    19201 gctgggattg aactagccaa ccaactgggt caactcaact tatcagacca tgaaaaattg
    19261 cagttagcga ccaagattga agggcatcct gacaatgtgg ctccagccat ttatggtaat
    19321 ctcgttattg caagttctgt tgaagggcaa gtctctgcta tcgtagcaga ctttccagag
    19381 tgtgattttc tagcttacat tccaaactat gaattacgta ctcgcgacag ccgtagtgtc
    19441 ttgcctaaaa aattgtctta taaggaagct gttgctgcaa gttctatcgc caatgtagcg
    19501 gttgctgcct tgttggcagg agacatggtg accgctgggc aagcaatcga gggagatctc
    19561 ttccatgagc gctatcgtca ggacttggta agagaatttg cgatgattaa gcaagtgacc
    19621 aaagaaaatg gggcctatgc aacctacctt tctggtgctg ggccgacagt tatggttctg
    19681 gcttctcatg acaagatgcc aacaattaag gcagaattgg aaaagcaacc tttcaaagga
    19741 aaactgcatg acttgagagt tgatacccaa ggtgtccgtg tagaagcaaa ataaagaata
    19801 gaagatagga tggggaaact cttgaccaga gggtttcata tcctttttgt gaaaagaagt
    19861 ttatactcaa tgaaaatcaa agagcaaact aggaagctag ccgcaggctg ctcaaaacag
    19921 tgttttgagg ttgcagatag aactgacgaa gtcagtaaca tacctacggt aaggtgacgc
    19981 tgacgtggtt tgaagagatt ttcgaagagt attagttaaa aacgtgataa aggagaaata
    20041 aagatggcag aaatttatct agcaggtggt tgtttttggg gcctagagga atatttttca
    20101 cgcatttctg gagtgctaga aaccagtgtt ggctacgcta atggtcaagt cgaaacgacc
    20161 aattaccagt tgatcaagga aacagaccat gcagaaacgg tccaagtgat ttacgatgag
    20221 aaggaggtgt cactcagaga gattttactt tattatttcc gagttatcga tcctctatct
    20281 atcaatcaac aagggaatga ccgtggtcgc caatatcgaa ctgggattta ttatcaggat
    20341 gaagcagatt tgccagctat ctacacagtg gtacaggagc aggaacgcat gctgggtcga
    20401 aagattgcag tagaagtgga gcaattacgc cactacattc tggctgaaga ctaccaccaa
    20461 gattatctca ggaagaatcc ttcaggttac tgtcatatcg atgtgaccga tgctgataag
    20521 ccattgattg atgcagcaaa ctatgaaaag cctagtcaag aggtgttgaa gaccagtcta
    20581 tctgaagagt cttatcgtgt cacacaagaa gctgctacag aggctccatt taccaatgcc
    20641 tatgaccaaa cctttgaaga ggggatttat gtagatatta cgacaggtga gccactcttt
    20701 tttgccaagg ataagtttgc ttcaggttgt ggttggccaa gttttagccg tccgatttcc
    20761 aaagagttga ttcattatta caaggatctg agccatggaa tggagcgaat tgaagttcgt
    20821 tctcgttcag gcagcgctca cttgggtcat gttttcacag atggaccgcg ggagttaggc
    20881 ggcctccgtt actgtatcaa ttctgcttct ttacgctttg tggccaagga tgagatggaa
    20941 aaagcaggat atggctatct attgccttac ttaaacaaat aaaacagaga gtggggcttc
    21001 ccactttctt catttctaga atatgaatag aagggattta tgaaacacct attatcttac
    21061 ttcaaaccct acatcaagga atcaatttta gcccccttgt tcaagctgtt agaagctgtt
    21121 tttgagctct tggttcccat ggtgattgct gggattgttg accaatcttt acctcaggga
    21181 gatcaaggtc atctctggat gcagattggc ctgctcctta tctttgcagt aattggcgtt
    21241 ttagtggcct tgatagctca attttactca gcaaaggcag cagtaggttt tgctaaggaa
    21301 ttgacaaacg atctttatcg tcatattctt tccttgccca aggacagcag agaccgtctg
    21361 acaacatcta gtttggtcac tcgcttgact tcggatacct accagattca gactggtatc
    21421 aatcaattcc tgcgtctctt tttgcgagcg cccattatcg tttttggtgc catttttatg
    21481 gcttatcgaa tctcaactga gttgactttc tggttcttag tcatggttgc cattttgacc
    21541 attgtcattg tagggttatc tcgattggtc aatcctctct acagtagtct cagaaagaaa
    21601 acggaccaac tggttcagga aacgcgccag caattgcaag ggatgcgggt tattcgtgct
    21661 tttggtcaag aaaaacgaga gttacagatt tttcaaaccc ttaaccaagt ttatgctaga
    21721 ttacaagaaa agacaggttt ctggtctagt ttattaacac ctctgaccta tctgattgtc
    21781 aatggaactc ttctcgttat tatctggcaa ggctatattt caattcaagg aggagtactc
    21841 agtcaaggtg ctctcattgc tcttatcaat tacctcttac agattttggt ggaattggtc
    21901 aagctagcca tgctgatcaa ttccctcaac cagtcctata tctcagccaa gagaatcgag
    21961 gaggtctttg cggaatctcc cgaaaacatc cattcagaat tagaacaaaa gcaagttacc
    22021 agtggtcggg ttttacaagt ccaagaattg acttttacct atcctgatgc ggcccagcct
    22081 tctctgagag acatttcctt tgatatgact caaggacaaa tccttggtat cattgggggg
    22141 actggttctg gtaaatcaag cttggtgcaa ctcttacttg gactttatcc agtagacaag
    22201 gggaacattg acctttatca aaatggacgt agtcctctta atttggagca gtggcggtct
    22261 tggattgcct atgtacctca aaaggtcaaa ctctttaagg gaactattcg ttccaacttg
    22321 actttaggtt taaatcaaga agtatctgac cagaaactct ggcaggcctt ggagattgcg
    22381 caagctaagg attttgtcag tgaaaaggaa ggacttttgg atgccctagt tgaggcaggg
    22441 gggcgaaatt tctcaggtgg acaaaaacaa aggttgtcta tcgcccgagc agtcttgcgc
    22501 caagctccgt ttatcatcct agatgatgca acctcggcac tggataccat tacagagtcc
    22561 aagctcttga aagctattag agaaaatttt ccaaacacga gcttaatttt gatctctcaa
    22621 cgaacctcaa ctttacagat ggcggaccag attctcctct tggaaaaagg tgagttgcta
    22681 gctgttggca agcacgatga cttgatgaaa tccagccaag tctatcgtga aatcaatgca
    22741 tcccaacatg gaaaggagga ctagaatgaa acgacaaact gtaaaccaga cgctcaaacg
    22801 tttagccgta gatttagcaa accatccctt cctccttttc ctagcctttc taggaactat
    22861 tgcccaagtt ggcttatcaa tttacctacc tattctgatt gggcaggtca ttgaccaagt
    22921 cctagtggct ggttcatcac cagttttttg gcagattttt ctccagatgc tcttggtggt
    22981 aataggaaat actctggtac aatgggccaa tcctctcctc tataatcgtc taatcttctc
    23041 ttataccaga gatttgcgag agcgaatcat ccataagctc catcgtttac cgattgcttt
    23101 tgtagatagg caaggtagtg gagagatggt tagtcgtgta accacggaca tcgaacagtt
    23161 ggcagctggc ttgaccatga tttttaacca atttttcatt ggtgttttga tgattctggt
    23221 tagtattcta gccatgctcc aaattcatct cctcatgact ctcttagtct tgctgttgac
    23281 gccactgtcc atggtgattt cacgctttat tgccaagaga tcctatcatc tcttccagaa
    23341 gcaaacagag acgaggggaa ttcagactca gttgattgaa gaatcgctta gccagcagac
    23401 tataatccag tccttcaatg ctcaaacaga atttatccaa agattgcgtg aggctcatga
    23461 caactactca ggctattctc agtcagccat cttttattct tcaacggtca atccttcgac
    23521 tcgctttgta aatgcactca tttatgccct tttagctgga gtaggagctt atcgtatcat
    23581 gatgggttca accttgaccg tcggtcgttt agtgactttt ttgaactatg ttcagcaata
    23641 caccaagccc tttaatgata tttcttcagt gctagctgag ttgcaaagtg ctctggcttg
    23701 cgtagagcgt atctatggag tcttagatag ccctgaagtg gctgaaacag gtaaggaagt
    23761 cttgaccagt gaccaagtta agggagctat ttcctttaaa catgtctctt ttggctacca
    23821 tcctgaaaaa attttgatta aggacttgtc tatcgatatt ccagctggta gtaagatagc
    23881 catcgttggt ccgacaggtg ctggaaaatc aactcttatc aatctcctta tgcgttttta
    23941 tcccattagc tcgggagata tcttgctgga tgggcaatcc atttatgatt atacacgagc
    24001 atcattgaga cagcagtttg gtatggtgct tcaagaaacc tggctcacac aagggaccat
    24061 tcatgataat attgcctttg gcaatcctga agccagtcga gagcaagtaa ttgctgctgc
    24121 caaagcagct aatgcagact ttttcatcca acagtttcca cagggatacg ataccaagtt
    24181 ggaaaatgct ggagaatctc tctctgtcgg ccaagctcag ctcttgacca tagcccgagt
    24241 ctttctggct attccaaaga ttcttatctt agacgaggca acttcttcca ttgatacacg
    24301 gacagaagtg ctggtacagg atgcctttgc aaaactcatg aagggccgca caagcttcat
    24361 cattgctcac cgtttgtcaa ccattcagga tgcggattta attcttgtct tagtagatgg
    24421 tgatattgtt gaatatggta accatcaaga actcatggat agaaagggta agtattacca
    24481 aatgcaaaaa gctgcggctt ttagttctga ataagccatt ctcttttgaa agtttatgga
    24541 cgaaaaaagt tgccttcgag tgactttttt gttacaatag ctagaaaaat tgttcactgt
    24601 aatactcaat gaaaatcaaa gagcaaacta ggaagctagc cgtaggttgc tcaaaacagt
    24661 gttttgaggt tgcagataga actgacgaag tcagtaacca cacctacggc aaagtgaatt
    24721 tgaagtggtt tgaagagagt acaacttgtc ttttagaaaa ggagcctata atgaaagtct
    24781 ttcagcatgt aaatatcgtg acttgtgatc aagatttcca tgtttatctt gatggaatct
    24841 tagcagtcaa ggattctcaa atcgtctatg tcggtcaaga taagccagcg tttttagaac
    24901 aagctgagca gattatagac tatcagggag cttggattat gcctggtttg gtcaattgtc
    24961 acacccattc tgcaatgaca ggtctgagag ggatccgaga tgacagcaat ctccatgaat
    25021 ggctcaatga ctatatctgg ccagcagaat ctgagtttac tcccgacatg actaccaatg
    25081 cggtcaaaga agccctaaca gagatgctcc agtcaggaac aacaaccttt aacgatatgt
    25141 ataatcccaa tggtgtggat atccagcaaa tttatcaggt ggtgaaaact tccaagatgc
    25201 gttgttattt ttctccgact ctcttttctt cagagacaga aacaactgct gagactataa
    25261 gcagaactcg atccatcata gacgaaatct taaaatataa aaatccaaat ttcaaggtta
    25321 tggtagcacc tcattctccg tatagctgca gtagagactt gctggaagcg agtttggaaa
    25381 tggcaaaaga gctaaatatt ccgctccatg tccatgtggc ggagaccaag gaggagtcag
    25441 gaattatcct caaacggtac ggcaaacgcc cccttgcttt tcttgaagaa ctgggttatt
    25501 tagatcatcc gtccgtattt gctcacgggg tcgaattaaa cgagagagaa attgaactct
    25561 tggcatcttc tcaagtggct atcgcccaca atcctatcag taacctcaaa ctggcatcag
    25621 gaattgctcc aattatccag ctccaaaaag cgggagtagt agtcggaatt gcgactgact
    25681 cggttgcttc caataacaat ctagatatgt ttgaggaagg aaggactgca gctcttcttc
    25741 agaagatgaa aagtggggat gccagccagt ttccaatcga aacagctctc aaggtactga
    25801 caatcgaagg ggctaaggtc cttggaatgg aaaatcagat aggaagtctg gaagtcggca
    25861 agcaagcaga ttttctggtc attcaaccac aagggaaaat tcatctccaa cctcaggaaa
    25921 atatgctgtc tcacctggtt tatgcagtta aatctagtga tgtagatgat gtttatatcg
    25981 ccggagaaca ggttgttaag caaggtcaag tcctgacagt agaactttaa aagaaaaatc
    26041 acgaaaaatt ttaaaaaaag tttgcaaaaa tcttgcattc tttttttgac tatgctatac
    26101 ttatatacgg tttaaaaaaa ctgcctaaga cagtagggga gctcgactca taaatatcct
    26161 accgaggaca aaacgtatca tgtaaaaaga agcgtattgt actttcgtgt ctaggtttgg
    26221 gcgcgttttt ctttttgaaa aattccccaa gcaaaataat tacggaggtg aacacactaa
    26281 tgagtgaagc aattattgct aaaaaagcgg aactagttga cgtagtagct gaaaaaatga
    26341 aagctgctgc atctatcgtc gttgtagacg ctcgtggttt gacagttgag caagatacag
    26401 ttcttcgtcg tgagcttcgt ggaagcgaag ttgagtataa agttattaaa aactcaatct
    26461 tgcgtcgtgc agctgaaaaa gctggtcttg aagatcttgc atctgtattt gttggaccat
    26521 ctgcagtagc attttctaat gaagatgtta tcgcaccagc gaaaatcttg aacgactttt
    26581 ctaaaaacgc tgaagcactt gaaattaaag gtggtgcaat cgaaggcgct gtcgcatcta
    26641 aagaagagat tcttgcactt gcaactcttc caaaccgcga aggacttctt tctatgctcc
    26701 tttctgtact tcaagcgcca gtgcgcaacg ttgctcttgc agtcaaagcg gttgcagaaa
    26761 gcaaagaaga cgcggcttaa tcttaagcta cacagcgtag cctagctacg aaaaaaacta
    26821 ttataaaatt taaaacttat ttggaggaaa taacaatggc attgaacatt gaaaacatta
    26881 ttgctgaaat taaagaagct tcaatccttg aattgaacga ccttgtaaaa gctatcgaag
    26941 aagaatttgg tgtaactgca gctgctcctg tagctgttgc tgcagctgat gcagctgatg
    27001 ctggtgctgc taaagattca ttcgacgttg aattgacatc tgcaggcgac aaaaaagttg
    27061 gcgttatcaa agttgtacgt gaaatcactg gtcttggtct taaagaagct aaagaacttg
    27121 ttgacggtgc accagcactt gttaaagaag gcgttgcaac tgcagaagct gaagaaatca
    27181 aagctaaatt ggaagaagct ggagcttcag ttactcttaa ataatagagt atcgcaattt
    27241 gattgcggaa tccctattta ccagtctttg agagcgataa gcgattgata gaaactgata
    27301 aattgatttg gtttcctgcc aaaaattctt tggcaggatt tttgttttaa taaactttcg
    27361 ggggtagaca ctagattgtc tacccctttt tttgtgtttc ggaggtaatt atgcaaaagg
    27421 aaggacagtc atcacgtcaa ttcatgaaga gtcatctcat gactatcatg ggagttgata
    27481 ttgtaaaagc aactcagtta gttgatgaaa tggaacaagt aggtttaatc cgatttgatg
    27541 aatttggaaa tgttggaatt ttagtcttgg agggacaata atgaagcgta ttaccgctaa
    27601 tcaatatcag acatcagagc gttattataa gctccccaaa cttttatttg agagtgaacg
    27661 gtataagaat atgaaaccgg aggttaaggt ggtttactca gttttaaaag atcggttgga
    27721 gttgtctttg agcaaaggtt ggattgatga ggatgggact atttatttga tttattccaa
    27781 ttcaaatttg atggcacttt taggctgttc aaagtcaaaa ttactctcca tgtgagtttg
    27841 aagtgacatt tttagatgat taccataaaa aacataacta cccactattt tacgaatcct
    27901 atcttcaaaa cgttatggaa ttccttgaaa gtcaagacat aaagaatggg gttgatgcct
    27961 ttgtagatga tcatcaaaat ctcgtttttg ttttatatgg acaaggctat cgagccgagg
    28021 gaaaagaggg aatacttaca acccaagtaa ctgtaaaagc ttatgatgaa gacaagaaac
    28081 cgattaactt cgcaaattta ttagattcct taatcgtgtc agaatatcaa atggaaccga
    28141 atctttggga ggtctcctat gattgatctc tatctaagta aaaatagccg aagaaatcaa
    28201 cttcttttag acttcttcca aaactatggc atcgaggtat cttgtcattc agtttctgaa
    28261 atgacaaagg acaaattaat tgagatgatg agctattctt cagattgttt tgagttttta
    28321 tctccaaatt tattacgttt taagaatcga gataacctaa aattaacaga tttcatcgaa
    28381 atgatattaa aaaatcctga actaaccatc agacttcctc ttgcggtttc aaataagcga
    28441 gtttatccaa gtctgaattt ggaagaggct agagctctat tgccaagaga tacgaaacaa
    28501 ttgatttaca tggcgcaaac acattattta tctaactaaa ggagaagcaa tatggctgaa
    28561 cgattttggg agaacttgtc cattattttg gctgaaagaa atatcagctg gattgagcta
    28621 accagaaaaa tgtttgcggg agagtttcac tatccaagtg aattgaatcg tttgtatcaa
    28681 aagattcgtc actacaagat ggaacaacga atgcctcaaa gtccatgggt agaaaggatt
    28741 gtgcaggtat tagatttaga ttatgaagat ttatttcgga ggtaactatg aaaagaataa
    28801 ttccagttta tatattccaa caagtaaatg tcctattggt atctctatac ttactgaaat
    28861 ttctttgtat cggtgagtta actatactac agattctata ttgtgcgtca ctcatttctt
    28921 ttttatggat gtatgcccaa cgtaaacaag cgcataaggt caatatgaaa ttaaggataa
    28981 aatggattgg tattggatta gccagtctac tgattataag tctatgtttt agtctaatcc
    29041 atgcgcaaga aagcacgaat caagcaaact taattgggct tcaatatcaa gttccttggt
    29101 tttcattttt attgttctta atcaatgcga gtatggttga agaatttctg tatagagaaa
    29161 ttttatggaa cttggttaga aaattagata ttcgagttgc tttgacaagt gttttatttg
    29221 ccttagcaca tcatccagga accattatag cttggtgttt gtatgtatcg ctgaggatgt
    29281 ttttagggct tgtacgctac aaattggact tatggggcag tatggggcta catttggtgt
    29341 ggaacctact agtttatagt ttgctgcttt tttaaaatag gtaacctgat ttttaggtaa
    29401 cttctaaaac taacttccag ttttgggaga gagatggaag ttactttgaa aagttacgga
    29461 aacattattt tctatgtgac aacattgtat cattgcttgc attttactgc tggagagagt
    29521 ataatgaatg tggataaagc acttaatccc aaaaatttaa ataggaggag ttagctatgg
    29581 cagaagaagt attaaatctt taattagtat ctgttcaagt tgacgaaaca gatgaagttg
    29641 atggaatgcg atttagtaca ttcagtacaa accgttgcgg taactggagt gcttttagtt
    29701 gggaaaattg ttaattcatt taaattgtaa actttagaga ttctctggag ttatggatgt
    29761 cgttccaata tgtgcacgtt ggaatgttag tgcttatatg gggggaatcc aaaggattct
    29821 ccctttttat tttaataagt ctgtctagct atagatatat taagtttgat agaatttctt
    29881 ggagattttg ttatggatta taatttcaat ttggagcatc ctttcttttt cactaataat
    29941 gattatagta cagatactag cataaagtac taagtaagtc tgccttttaa ttggcacgaa
    30001 gtaatgaata atgatgagtg ggtttatcaa taccctattg gaaaatttgt tgaaagacaa
    30061 gggtggaaaa tacacattag ttcagagtat aatagttcac acgaattgtt acaagatgta
    30121 gcaaaaattt gccatgaaat gcgaattcca tttaaacatc ttagtacaga agacaaattt
    30181 atcatgcgaa acgggaaatt ggttagtcga ggattttctg gtaaatttat tacatgttat
    30241 ccaaatcaaa atgaattaga gtcggttttg caaaagttag aaagtgcctt aaagcaatat
    30301 aatgggccat atattttaag cgataaacgt tgggatgaag taccaatata tctgaggtat
    30361 ggtgtgttta ggccgtcaag ggatgatgaa aagaaggttg ctattgatga attaattgtt
    30421 ggcgatgaag ttgttaaaga tgaacgttta ccagtattta aaattccaaa aggtatagtg
    30481 ctccctgact ttttgaataa atggcttgac aagaaagata aaaaacaggg agactttccc
    30541 tttattatag ataatgcaat tagattttca aacagtggtg ggatatataa tgctagacta
    30601 aaagaagatg ggaaaaaaat aattttaaaa gaggctagac cgtatacagg tctaggattt
    30661 gatggtactt attcttcaga gagattagct tcggagtgca aagcactaaa aattctaaat
    30721 gaatggagtg agacgcctaa aatatactgg tgcggtaaga tctgggagca caccttctta
    30781 ggaattgaac atatgaaggg cgttccacta aatagatggg ttaccaataa ttttccttta
    30841 tatgaagttg ttgataagac caaagattat ctattgagag tttcaaagat agtagaaaaa
    30901 ctcatcgatt taacgaataa gtttcattct gaaaatgtat atcatcaaaa tttacattta
    30961 ggaaatatct tggttaagga tgaagatgag attagtataa tagactggga acaggctgtc
    31021 ttttcgaatg atgaaaaagt tgtacataaa gttgcagccc ctggttttag agcatggcga
    31081 gaaacacttc ccagtgagat agattggtat ggtattagac aaatagctca ttatttatac
    31141 atgccactag taactacatc ggatttgaca tacaactatg taagtcaaac caggatagaa
    31201 ggaaaaaaac tatttgagtc tttgggttat acaagagaac atatagatta tgtagaaagt
    31261 ttattgtcat atctagattc taaatgtcca cagattgaga atatttccag gaaaaaagtt
    31321 ttaaaaccca tgcatgagat tagaactatc gagagtgagc aagatataca agattttatt
    31381 ataaaagcct ttcttttagt gaactgatag gtattttctt agcaaacgat aaagttttag
    31441 ggccgataca atcaattgcc tattcattaa ataagataaa tacaaccaaa gatttaagga
    31501 aaccgttttt aaaatactta agtggagaga agaattttat agacgctgaa catgataata
    31561 acggactgta tacttcatca atagatgaga tacacatgaa agatgttgta tattctatta
    31621 caccagaaaa taaattaagt attgacttct catttaagtc accatttagg gtattattaa
    31681 caggaacttc tggtagtggg aaaacaacga ttttaaattt aattaatggt tctttaaagc
    31741 cacaaaaagg ttatgtaaat ttgttatcac atgggaaaaa gagttcagat tcaataccaa
    31801 cagttgatca gacaccatat atttttgaca ctactattcg tgagaacgta actttatttc
    31861 aaaatgaata tttttcagat gatcagataa ttgaggtgtt aaaaaaggta aatctatatg
    31921 aagaattaga aaagatagat atactaaatt atcaatgtgg tgaaaatggt agtaatttgt
    31981 ctggaggtca aaaacaaaaa atagctttag ctagagctct gattagaaat aataaagtgt
    32041 acttatttga cgaaatatca gctaatttag ataatgataa ttcaaattcc atacatgata
    32101 ttctgttcaa tttaggtatt tcatttattg aagtttcaca tcattatgac ttaaatgaca
    32161 agagatacac tgatatatat aaattggaaa atggaacact tttcaaaatt aagtgattgg
    32221 aggcaagaat atggataaag attatatatt aaaagtgaaa gggctgtatc atcaattttt
    32281 actaggaaat aataaaacgt tgcaagtgct gaaaaatgtt tctctttctg cttcgagagg
    32341 agaatttata agtattctag gaattagtgg ttctggaaag tcaactttat taaaatgtat
    32401 ttctagtttg cttgaaccaa caagtgggga agtaatttta aatggaatca acccctataa
    32461 aatcagaaat gcaaaattgt cgagtataag acgtaacgaa gtatctttta tatttcaagc
    32521 atacaattta ataccttccc tgccggtaat agaaaatata gcacttcctt tgcgattatc
    32581 acaaaaaaaa ttaactatta aaaatgtaga aaacttactc aaaagaatga agtttaatgc
    32641 tggcttaaac gattttgttg gaactctgtc tggtggagaa caacagaagg ttgctatagc
    32701 tagagcggtt attgctgata gtgatataat atttgctgat gagccaactg gggctttaga
    32761 cagcgtttct cgtgaagtaa tttttgaatt attgagagag ttagtagggg cgggtaagtg
    32821 tgtaattatg gtaacgcacg atatagaatt ggcctcgaaa actgatcgtg cattaatatt
    32881 gaaagatgga aaaattttca aagaacttca tagacctagc ggggaagagt tgtataaaat
    32941 cttagaggta caatcaacta cggaggaata gagatgctca gattagttta ctatcaattt
    33001 ttacataata agaaacaatg gttaggcgtt tcaccagtaa tttttgtttc gagtttagtt
    33061 atgggattgg ctgttaatgg ggtaattaat gttgagaata attcgcaagt atttgtcgga
    33121 cttccagatc ctaaaccaat ttttatgttt ccgatagtat tcggaggagt aacattattc
    33181 tttgtgctct ctaacataat aaatatgttg gttgaaattt ttagagatga ttacgaactt
    33241 ctggaggtgt tgggagcaag tctccttcaa ctatcatttc ttgtgggggg acagattttt
    33301 atcatttcct cgattattag ttttattgca tatctatgct ctatatttgt tacgtcaaat
    33361 tattattatt ttttacaata ctttttcggg gaaaatatat tacctgatat ccaatttcaa
    33421 acgagtgctg ttggatgtat cattacagta gtgttgattt catttttagc ctttctaagt
    33481 ggttgctttt atacgttcaa gaagattaga aataggaaga gttctaaaat aaggcatgtt
    33541 ctttcaatag taaaaagaat actgctattg gctggtttta gtgttatctg gttgctttct
    33601 ttgcaacaaa ttttccaaga tagcacaatt ttagcgaagg cccagataat attcaatatt
    33661 gtaatcttag atatt
//
LOCUS       DBERMR010000021        33205 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.021, whole genome shotgun sequence.
ACCESSION   DBERMR010000021 DBERMR010000000
VERSION     DBERMR010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 33205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33205
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.021"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            50..475
                     /locus_tag="ACL8A1_001050"
                     /pseudo
     CDS             50..475
                     /locus_tag="ACL8A1_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            488..1519
                     /locus_tag="ACL8A1_001051"
     CDS             488..1519
                     /locus_tag="ACL8A1_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1201837.1"
                     /translation="MSEIKIINAKKIYHDVPVIENLNITIPKGSLFTLLGPSGCGKTI
                     LLRMIAGFNSIEGGEFYFDDTNINNMEPSKRNIGMVFQNYAIFPHLTVRDNVTFGLMQ
                     KKVPKEELIQQTNKYLELMQIAQYADRKPDKLSGGQQQRVALARALAVNPSVLLMDEP
                     LSNLESKLRLDIHQAIREIQHEVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRP
                     KELYHKPANEFVATFIGRTNIIPANLEKRSDGAYIVFSDGYALRIPALDQVEEQAIHV
                     SIRPEEFIKDESGDIEGTISDSVYLGLNTEYFIETGFASKIQVSEESTFEEDLQKDNR
                     IRLRINTQK"
     gene            1569..3221
                     /locus_tag="ACL8A1_001052"
                     /pseudo
     CDS             1569..3221
                     /locus_tag="ACL8A1_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(3324..4016)
                     /locus_tag="ACL8A1_001053"
     CDS             complement(3324..4016)
                     /locus_tag="ACL8A1_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HGS1201838.1"
                     /translation="MVKVRLYLIRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(4260..5597)
                     /locus_tag="ACL8A1_001054"
     CDS             complement(4260..5597)
                     /locus_tag="ACL8A1_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HGS1201839.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGKEGDMIEFGGLLGTAPVMRVNGASSVDFISRGGQIPAPIHSFKN"
     gene            complement(5607..5873)
                     /locus_tag="ACL8A1_001055"
     CDS             complement(5607..5873)
                     /locus_tag="ACL8A1_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HGS1201840.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAIVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            complement(6138..6524)
                     /gene="rplQ"
                     /locus_tag="ACL8A1_001056"
     CDS             complement(6138..6524)
                     /gene="rplQ"
                     /locus_tag="ACL8A1_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HGS1201841.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIVELV"
     gene            complement(6536..7471)
                     /locus_tag="ACL8A1_001057"
     CDS             complement(6536..7471)
                     /locus_tag="ACL8A1_001057"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HGS1201842.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            complement(7514..7897)
                     /gene="rpsK"
                     /locus_tag="ACL8A1_001058"
     CDS             complement(7514..7897)
                     /gene="rpsK"
                     /locus_tag="ACL8A1_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HGS1201843.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(7915..8280)
                     /gene="rpsM"
                     /locus_tag="ACL8A1_001059"
     CDS             complement(7915..8280)
                     /gene="rpsM"
                     /locus_tag="ACL8A1_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HGS1201844.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(8298..8414)
                     /gene="rpmJ"
                     /locus_tag="ACL8A1_001060"
     CDS             complement(8298..8414)
                     /gene="rpmJ"
                     /locus_tag="ACL8A1_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HGS1201845.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            complement(8439..8657)
                     /gene="infA"
                     /locus_tag="ACL8A1_001061"
     CDS             complement(8439..8657)
                     /gene="infA"
                     /locus_tag="ACL8A1_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HGS1201846.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(8774..9412)
                     /locus_tag="ACL8A1_001062"
     CDS             complement(8774..9412)
                     /locus_tag="ACL8A1_001062"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HGS1201847.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLN
                     ELGIELEGVINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            complement(9563..10873)
                     /gene="secY"
                     /locus_tag="ACL8A1_001063"
     CDS             complement(9563..10873)
                     /gene="secY"
                     /locus_tag="ACL8A1_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HGS1201848.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            complement(10886..11326)
                     /gene="rplO"
                     /locus_tag="ACL8A1_001064"
     CDS             complement(10886..11326)
                     /gene="rplO"
                     /locus_tag="ACL8A1_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HGS1201849.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            complement(11471..11653)
                     /gene="rpmD"
                     /locus_tag="ACL8A1_001065"
     CDS             complement(11471..11653)
                     /gene="rpmD"
                     /locus_tag="ACL8A1_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HGS1201850.1"
                     /translation="MAQIKITLTKSPIGCIPSQRKTVVALGLGKLNSSVIKEDNAAIR
                     GMITAVSHLVTVEEVN"
     gene            complement(11667..12161)
                     /gene="rpsE"
                     /locus_tag="ACL8A1_001066"
     CDS             complement(11667..12161)
                     /gene="rpsE"
                     /locus_tag="ACL8A1_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HGS1201851.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            complement(12179..12535)
                     /gene="rplR"
                     /locus_tag="ACL8A1_001067"
     CDS             complement(12179..12535)
                     /gene="rplR"
                     /locus_tag="ACL8A1_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HGS1201852.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            complement(12619..13155)
                     /gene="rplF"
                     /locus_tag="ACL8A1_001068"
     CDS             complement(12619..13155)
                     /gene="rplF"
                     /locus_tag="ACL8A1_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HGS1201853.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEVTLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            complement(13347..13745)
                     /gene="rpsH"
                     /locus_tag="ACL8A1_001069"
     CDS             complement(13347..13745)
                     /gene="rpsH"
                     /locus_tag="ACL8A1_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HGS1201854.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            complement(14030..14299)
                     /gene="rpsN"
                     /locus_tag="ACL8A1_001070"
     CDS             complement(14030..14299)
                     /gene="rpsN"
                     /locus_tag="ACL8A1_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HGS1201855.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            complement(14317..14859)
                     /gene="rplE"
                     /locus_tag="ACL8A1_001071"
     CDS             complement(14317..14859)
                     /gene="rplE"
                     /locus_tag="ACL8A1_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HGS1201856.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            complement(14883..15188)
                     /gene="rplX"
                     /locus_tag="ACL8A1_001072"
     CDS             complement(14883..15188)
                     /gene="rplX"
                     /locus_tag="ACL8A1_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HGS1201857.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            complement(15266..15634)
                     /gene="rplN"
                     /locus_tag="ACL8A1_001073"
     CDS             complement(15266..15634)
                     /gene="rplN"
                     /locus_tag="ACL8A1_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HGS1201858.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            complement(15660..15920)
                     /gene="rpsQ"
                     /locus_tag="ACL8A1_001074"
     CDS             complement(15660..15920)
                     /gene="rpsQ"
                     /locus_tag="ACL8A1_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HGS1201859.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            complement(15945..16151)
                     /gene="rpmC"
                     /locus_tag="ACL8A1_001075"
     CDS             complement(15945..16151)
                     /gene="rpmC"
                     /locus_tag="ACL8A1_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HGS1201860.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            complement(16161..16574)
                     /gene="rplP"
                     /locus_tag="ACL8A1_001076"
     CDS             complement(16161..16574)
                     /gene="rplP"
                     /locus_tag="ACL8A1_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HGS1201861.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            complement(16578..17231)
                     /gene="rpsC"
                     /locus_tag="ACL8A1_001077"
     CDS             complement(16578..17231)
                     /gene="rpsC"
                     /locus_tag="ACL8A1_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HGS1201862.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(17244..17588)
                     /gene="rplV"
                     /locus_tag="ACL8A1_001078"
     CDS             complement(17244..17588)
                     /gene="rplV"
                     /locus_tag="ACL8A1_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HGS1201863.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            complement(17600..17881)
                     /gene="rpsS"
                     /locus_tag="ACL8A1_001079"
     CDS             complement(17600..17881)
                     /gene="rpsS"
                     /locus_tag="ACL8A1_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HGS1201864.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            complement(17985..18818)
                     /gene="rplB"
                     /locus_tag="ACL8A1_001080"
     CDS             complement(17985..18818)
                     /gene="rplB"
                     /locus_tag="ACL8A1_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HGS1201865.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            complement(18836..19132)
                     /locus_tag="ACL8A1_001081"
     CDS             complement(18836..19132)
                     /locus_tag="ACL8A1_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HGS1201866.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(19132..19755)
                     /gene="rplD"
                     /locus_tag="ACL8A1_001082"
     CDS             complement(19132..19755)
                     /gene="rplD"
                     /locus_tag="ACL8A1_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HGS1201867.1"
                     /translation="MANVTLFDQTGKEAGQVVLNDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQVAISKIEEVLA"
     gene            complement(19780..20406)
                     /gene="rplC"
                     /locus_tag="ACL8A1_001083"
     CDS             complement(19780..20406)
                     /gene="rplC"
                     /locus_tag="ACL8A1_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HGS1201868.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            complement(20623..20931)
                     /gene="rpsJ"
                     /locus_tag="ACL8A1_001084"
     CDS             complement(20623..20931)
                     /gene="rpsJ"
                     /locus_tag="ACL8A1_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HGS1201869.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(21197..21814)
                     /locus_tag="ACL8A1_001085"
     CDS             complement(21197..21814)
                     /locus_tag="ACL8A1_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HGS1201870.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRHLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKAGEVLSGTKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            complement(21811..22401)
                     /gene="nrdG"
                     /locus_tag="ACL8A1_001086"
     CDS             complement(21811..22401)
                     /gene="nrdG"
                     /locus_tag="ACL8A1_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HGS1201871.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            complement(22394..22900)
                     /locus_tag="ACL8A1_001087"
     CDS             complement(22394..22900)
                     /locus_tag="ACL8A1_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1201872.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKSQSAHDGGFWDTENFVYEEWL
                     ETNIQKEMGINLPENRVPSIQFVLFGESGCALRFLNLRLRLNERLLNYAGHIGYSIRP
                     SEKGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDAHNGVER
                     YWIEVANE"
     gene            complement(22944..23084)
                     /locus_tag="ACL8A1_001088"
     CDS             complement(22944..23084)
                     /locus_tag="ACL8A1_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201873.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            complement(23096..25303)
                     /gene="nrdD"
                     /locus_tag="ACL8A1_001089"
     CDS             complement(23096..25303)
                     /gene="nrdD"
                     /locus_tag="ACL8A1_001089"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HGS1201874.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKWEDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHHLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            complement(25423..26961)
                     /locus_tag="ACL8A1_001090"
     CDS             complement(25423..26961)
                     /locus_tag="ACL8A1_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HGS1201875.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            complement(26998..27141)
                     /locus_tag="ACL8A1_001091"
     CDS             complement(26998..27141)
                     /locus_tag="ACL8A1_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201876.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            complement(27219..28751)
                     /gene="cls"
                     /locus_tag="ACL8A1_001092"
     CDS             complement(27219..28751)
                     /gene="cls"
                     /locus_tag="ACL8A1_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HGS1201877.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            complement(28897..29352)
                     /locus_tag="ACL8A1_001093"
     CDS             complement(28897..29352)
                     /locus_tag="ACL8A1_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0198 family lipoprotein"
                     /protein_id="HGS1201878.1"
                     /translation="MKLKRFTLSLASLASFSLLVACSQRAQQVQQPVAQQVQQPAQQN
                     TNTANAGGNQNQAAPVQNQPVAQPTDIDGTYTGQDDGDRITLVVTGTTGTWTELESDG
                     DQKVKQVTFDSANQRMIIGDDVKIYTVNGNQIVVDDMDRDPSDQIVLTK"
     gene            complement(29436..30686)
                     /locus_tag="ACL8A1_001094"
     CDS             complement(29436..30686)
                     /locus_tag="ACL8A1_001094"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HGS1201879.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRIQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLADQVKE
                     MEKTLLQTPDQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLICQKKAESLAVDLYQA
                     GQDFSVLNGDFSSSLLNLSQLNIGLEGAYQQENAALALQTFLLFMGERKEAIDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTCLVEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            complement(30993..31298)
                     /locus_tag="ACL8A1_001095"
     CDS             complement(30993..31298)
                     /locus_tag="ACL8A1_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HGS1201880.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            complement(31314..31733)
                     /gene="ruvX"
                     /locus_tag="ACL8A1_001096"
     CDS             complement(31314..31733)
                     /gene="ruvX"
                     /locus_tag="ACL8A1_001096"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HGS1201881.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            complement(31737..32003)
                     /locus_tag="ACL8A1_001097"
     CDS             complement(31737..32003)
                     /locus_tag="ACL8A1_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HGS1201882.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            complement(32090..32659)
                     /locus_tag="ACL8A1_001098"
     CDS             complement(32090..32659)
                     /locus_tag="ACL8A1_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP0191 family lipoprotein"
                     /protein_id="HGS1201883.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQQGVE
                     ETQKALLEAEEKDKSIIEARKLVGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            complement(32725..33123)
                     /gene="spx"
                     /locus_tag="ACL8A1_001099"
     CDS             complement(32725..33123)
                     /gene="spx"
                     /locus_tag="ACL8A1_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HGS1201884.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
BASE COUNT         9403 a         7380 c         6076 g        10346 t
ORIGIN      
        1 aacaatgttt tagaagtaga ggtgtactat tctagtttca atacactata aaatcggttc
       61 aagttcatct ggtgtctata aagcagtcgt tgatggagaa atggctgttg gtctctctta
      121 tgaggatcca gcagttaaac tcttaaatga cggagctaac attaaagtag tctatccaaa
      181 agaaggaacc gtcttcctac ctgctagtgc tgctatcgtt aaaaaagcta aaaatatgga
      241 aaatgccaag aaatttatcg attttattat ctctcaagaa gtacaagata cacttggtac
      301 aaccactact aaccgtcctg ttcgtaaaaa tgctaaaaca agcgaaaaca tgaaaccaat
      361 tgacaaaatc aaaacactca ctgaagatta tgattatgat tatgtcatca agaataaatc
      421 agatatcgtt aagaaataca acgaagtctt tacagatatc caatctaaac agtaaaagag
      481 gttcactatg agtgagatca aaattattaa cgccaaaaaa atctaccacg atgtccctgt
      541 tattgagaat ttgaacatta caattccaaa aggaagtctc tttacccttc ttggaccttc
      601 aggatgtggg aaaacgatcc ttcttcgtat gattgcaggt ttcaacagta tcgaaggtgg
      661 agaattttac ttcgatgata caaacatcaa taatatggaa cccagcaaac gcaatatcgg
      721 gatggttttc caaaactacg ctattttccc acatttgact gtccgagaca acgttacttt
      781 tggtcttatg caaaagaagg ttccaaaaga agaattgatt caacagacca acaagtatct
      841 tgaacttatg caaattgctc aatatgcgga tcgaaagccc gataaactca gtggtggaca
      901 acaacaacgt gtcgccttgg cacgcgcctt agcggttaat ccaagtgttc tcctcatgga
      961 cgagccactt agtaatctgg agtccaaact tcgcttggat atacatcaag ccatccgaga
     1021 aatccaacac gaagtgggaa ttacaactgt ttatgtaacc cacgaccaag aagaagccat
     1081 ggctatttca gaccaaattg ctgttatgaa agatggggtg atccaacaaa tcggccgacc
     1141 aaaagaactc tatcataaac cagctaatga gtttgtagca acctttatcg gacgcacaaa
     1201 tattatccct gccaatcttg aaaaacggag cgacggcgct tatatcgtct tttcagatgg
     1261 ctatgccctt cgaataccag ctcttgatca ggttgaggag caagctattc atgtaagcat
     1321 tcgtcccgaa gagtttatca aagatgaatc tggagatatc gaaggaacta ttagcgatag
     1381 cgtctatctt ggactaaata cggagtattt cattgagaca ggttttgcct caaaaattca
     1441 agttagtgaa gaatcaactt ttgaagaaga tctacaaaaa gacaatcgta ttcgtctacg
     1501 aatcaatacg caaaaatgaa acatcttttc tgcagatggt tcccaaaacc tgataaaagg
     1561 agtcaaccat ggaatgtaaa aaactaaata tttggacagc ctcctctttc ttcatctttc
     1621 ttacctatct tgtctttctc gtttatccta tcgttaccgt gctcaagtaa gcacttatac
     1681 atgaaggata attctcacta gctaattttg tcactttctt tagtaaagcc tactactctg
     1741 agacactagt caacagtttc agggtttcca ttaccgctac tgtcacttcc ttagttgtag
     1801 gaaccctatt agcttatctc ttctctatgt atgacttcaa ggggaagaaa tttctacaaa
     1861 tattgattat cattgcttcc atgtcagctc ctttcgtagg agcctactcc tggattctct
     1921 tgctgggacg aaatgaggtc atcactaaat ttttgacaaa tgcccttcat cttccagcta
     1981 tcgatattta tggattcaaa ggaattatac ttgtctttac actgcaacta ttcccactgg
     2041 tatttctata tgttgctgga acaatgaaca gtattgacaa ttctctattt gaagcagctg
     2101 aaagcatggg gtccttcgga tttaaaccta tcgtaacggt tgttttacct ctcctagttc
     2161 caaccttact agcagctcct tgcttgtatt tatgagagca ttctcagact ttggaacgcc
     2221 tatgttgatt ggcgaaggat atcggacttt ccctgtcctg atttataccc aatttattag
     2281 cgaggttgga ggaaattctg cttttgcaat tatggcgatt atcattgcct tggcaatttt
     2341 ccttatccaa aaacacattg caaaccgcta cagtttcagc atgaatccgc tccatccaat
     2401 tgagcctaaa aaaactacaa aaggaaaaat ggctgccatt tatgcaacag tctacggaat
     2461 tatctttatc tctgttttac ctcaaatcta cttaatttat acctctttcc taaaaacatc
     2521 aggtatggta tttgttaaag gttattctcc aaatagttac aagctagctt tcaatcgtat
     2581 gggatctgct attttcaata ccattcgtat ccctttgatt gccttagttc tagttgttct
     2641 atttgcgaca tttatctcct acctagccgt tagaaaacgg aatttgttta caaacttaat
     2701 tgacagcctc agtatggtac cttatattgt accaggaacc gttctaggga ttaccttcat
     2761 ttcttccttc aatactggtc tatttggaag tggatttctt atgattacag ggactgcttt
     2821 catcttgatt atgtctctat ctgtcagaag attaccttat actattcgct catctgttgc
     2881 tagcttacaa caaatagcac caagtattga agaagctgct gaaagcttag gaagtagtcg
     2941 tctcaatacc tttgctaaga ttacaactcc aatgatgcta tctggtatca tttctggagc
     3001 catcttatct tgggtcacaa tgatttcaaa actctctact tctatcctct atcctcctct
     3061 acaatgtcaa aacaagaaca atgactgtag ctatttatac agaggttctc agaggaaatt
     3121 acggtgtagc cgcagccttg tcaactatcc tgactgttct aacagtaggt tccttgctct
     3181 tgtttatgaa aatctctaaa agcaatagca ttacacttta gttttccaca tcaaaaacag
     3241 ccgaaaggag taccctcggc tgttttttct tatcttgata ttcttatcaa atccctagct
     3301 catggcaagc aagtctagac tgattaaata gagccttctt ccatcttctc acgtcctagc
     3361 tctcggtaac tacggtcacc gacaacttca accctaaact gaccgtcctc atattcaaga
     3421 atcgtcacgc taccattatc tagaccatgc ggatgcatgc cattaatcag ataaacaatg
     3481 gttccaatag tcattccatg gctgacaaca agggcgttac ctccaccttg atcttccatt
     3541 tcttttgcaa tcatttcaaa gccttccttg attcggccac tgagtttttc ccagccttca
     3601 gcccaaccag ctgtatcgac ctctaccaag ccctcagcca gttcagcata agacaattgg
     3661 tggacgtggt ccacattaaa gatacgagga ataatgccca tgaaaagatc gccatcatag
     3721 gctccatcaa aactaccgaa acaccattct ctgatacgct tgtccatgcg ataagggatt
     3781 ttcccctgca agccaagttc ttcaagtata attcccatgg tctgaatggt acgaccagaa
     3841 tcactcgaat aagcgcgctc aaactgtaga tcagattctc gcaaaccgat tcctaactct
     3901 tgaatccctc gttcaccttc agcagttaag ggagtatcgc tccaaccttg cgcgcgacca
     3961 atcgtgttaa acatggtctt gccatgacgt atcaaataca atcgtacttt taccattttc
     4021 cgtcctccgt ttgcttctat tatatcatga atgataaaat aaaagccacc catacaggcg
     4081 acttttgaag gagattatta tgaaaaagtt taggagttca ttaaataaag atattagatg
     4141 aaaatcaaat tcaaactaat tcagtgttat ctgtttcaaa taatattagg aggtctttat
     4201 ttaatgatta tagtatacac atctaatctt aaatagaact taaaatttct cctattttct
     4261 taatttttaa aactatgaat tggtgctggg atttgtccac cgcgagagat gaagtcgaca
     4321 gacgaagccc cattaaccct cataacgggt gcagttccta atagaccacc aaactcaatc
     4381 atatcgcctt cttttccttt gggaatgata cgaacagctg ttgttttcat gttgataaca
     4441 ccgattgctg cttcatccgc aatcatagcc gcaatggttt cagcaggcgt atcttctggg
     4501 atggcaatca tatccaatcc aacagaacag atagccgtca tagcttctag tttttctaaa
     4561 ttaagagagc cattttgcac tgcagcaatc attccttcat cctcagaaac agggataaag
     4621 gcaccagata aaccaccgac ttgattgcag gccatcactc cacccttttt aacttggtcg
     4681 ttcaagaggg ccaaggcagc cgtcgttcca tgcgtgccaa ctgtttctag ccccatttcc
     4741 tcaaggacac gtgccacaga gtctccaacc gcaggggttg gtgccaaact caagtccaca
     4801 ataccaaact ccacacccag tctctcactg gccatttgac caaccaattg accgatacga
     4861 gtgattttaa aggcagtttt cttaactgtt tcggctacta catcaaagct ctgtccacga
     4921 actttttcca aagcacgttt cacaacacca ggaccagaaa ctccgacatt gatgataaca
     4981 tctgcttccc caacaccatg aaaggcaccc gccataaatg gattgtcctc aacagcatta
     5041 gcgaatacaa ccaacttggc cactcccata tctgaaagat ttgctgtttc cttgataatt
     5101 cgtcccatat ctgccacagc cgtcatatta ataccagact tggttgagcc gatattgact
     5161 gacgagcaga ccttatccgt ctcagccaaa gcgcgaggaa tggaattgat gagaatctca
     5221 tctccctttt gataaccttt ttgtactaag gcagaaaaac caccaataaa gtccacacca
     5281 atctctttcg cagccttatc aagcgctttt gccagaacca cgtagtccgt cgcatctgtc
     5341 gctgccccaa tcagagaaat aggtgtcacc gatacacgct tattaacgat aggaattccc
     5401 aactcagccg caatttcatc accaacagct actaaattag ccgcctttgt cgtaattttt
     5461 tgatagattt tctccgcagc acgattgata tctggatcga tacagtccaa aagagaaatc
     5521 cccatggtaa tggttctgat atcgaaattt tgctcctcaa tcatggcgat ggtttcagta
     5581 acttgtctaa tatccatgat aacctcctag atattataca tagcttcgaa aatcgctgca
     5641 ctctgaatat tgatttttac attcaaagtt tgcccaaaag cttcaaattc attacgaaga
     5701 taggtaaaat cttgcttttc atcactagat acaatagcca tcatcgtaaa atattcatcc
     5761 aagacagttt gagagatatc gtcaatattc aatcctaatt ctgcaatttt accagaaaca
     5821 cctgcaacaa ttccagattt atctttacca acaacagtta taatcgcttt cataagcaaa
     5881 ctccaattct tcttaaaata agaaacctta acattaaaca ggattctttt caaatagtat
     5941 agcataattc taacgaaaat actcaaaaac gcctgcttac gaagttgttc ttaagaaaaa
     6001 aacaatttcg taaacaagcg tctactatcc caacttatga tgagtgttcc cgctaggacc
     6061 gaaatcctag cggtagacca gagctagact aagagcacaa gactccatca tcataacact
     6121 caacaaaatt gatgatttta tactaattcg acgatcgcca ttggcgctgc atcaccacga
     6181 cgtggttcag ttttaaggat acgagtgtat ccaccgttac gttcagcata acgaggtgcg
     6241 atttctgaga acaatttttg aagtgctgta gtagaagtgt acttatcagt tgcttcatca
     6301 tagttttcag atgcgatttc attacgtacg aaagcagctg cttgacgacg tgcatgcaaa
     6361 tcaccacgtt tacctagagt aatcattttt tcaacagttt tacggatttc tttagcacga
     6421 gcttcagttg tcacgattga ttcgttgatc aaaaggtcag ttgtcaaatc gcgaagcatt
     6481 gctttacgtt gtgagctagt gcgtcctagt ttacggtaag ccatgtattc ctcctttatt
     6541 tatcttttaa tccaagaccc aaatcaatga gtttgagttt cacttcttcc aaactcttgc
     6601 gtccaagatt tcgtactttc atcatctctg cttcagattt ttctgtcaaa tcatgcacag
     6661 tattgatacc ggcacgtttt aaacagttgt atgaacgcac agacaagtcc agttcctcaa
     6721 tcgtacgatc taaaatacgg tcgtcagatt cagtatcagc ttctttcatc acttcagttg
     6781 acttagcaat ctcagtaaga tttgtaaaca aatcaagatg ttctgtcaaa atacgtgctg
     6841 aaagccctaa agcatcttct ggaataattg ttccatttgt caagatttca agggttaatt
     6901 tgtcgaaacc atcattgcta cctacacgag caggttccac ttgatagttg acttttgtaa
     6961 ctggtgtata aatagaatct acagcaagtg ttccaactgg tgcattatcc tttttatttt
     7021 catcagcagg tacatatcca cgaccactgt taacagtcat agtcgctttt agagaagaac
     7081 cttcaccgat tgtaaagaga taatgatctg gatttacaat ttcaatatcg ctatctgtca
     7141 aaatgtcacc agctgttact tcagcaggac cttcaacatc cagttcgatg attttttcgt
     7201 cttcaacgta cgatttcact gcaattcctt taatgttcag aatgatttgc atcacgtctt
     7261 cacgaacacc tggaactgtg tcaaactcat gtaacacacc atcaatgttg atagatgtca
     7321 cagctgctcc tggtagagaa gctagaagta cacgacgaag agagttacca agagttgtac
     7381 cgtagccacg ttcaagtggt tcgattacaa acttgccata atctttattt tcatcaattt
     7441 ttgttatatt tggtttttca aactcgatca tttagttact ccctcttaaa cgaaaagcag
     7501 tgtaatgcga tgattataca cggcgacgtt ttggaggacg agcaccattg tgtggcactg
     7561 gagtcacatc acgaattgct gttacttcaa gaccagcggc agcaagcgca cgaatagctg
     7621 actcacgacc agaacctgga ccttttacag taacttcaac tgatttaaga ccgtgttctt
     7681 gtgcagattt agcagcagct tcagaagcca tttgagcagc gaatggtgta gatttacgag
     7741 aacctttgaa accaagagca ccagctgatg accaagcaat tgcattacca tgcacatcag
     7801 taatcataac aatagtgtta ttaaatgtag cgtgaatatg agcaatacca gattcgatat
     7861 tctttttcac acgacgttta cgtgttggtt tagccaagac ttttacctcc tatattattt
     7921 tttcttacca gcaatcgcaa cagctttacc tttacgagtg cgagcgttgt ttttagtgtt
     7981 ttgtccacgg acaggaagtc cacgacggtg acggatacca cggtatgaac cgatttccat
     8041 caaacgtttg atgttcaagt ttacttcacg acgaaggtca ccttcaactt tgattgcatc
     8101 cacttcacga cggatagcat cttcttgatc tgatgtaaga tcacgtacac gaacatcttc
     8161 tgagattcca gcagcagcca aaattttctt agatgttgca agtccgatac cataaacata
     8221 agtcaatgag attactacgc gtttgtcatt tggaatatca actccagcaa tacgagccat
     8281 gttttctcct ttctatctta tccttgacgt tgtttgtgtt ttggatttgc tgggcaaatt
     8341 accataacac gaccattacg acgaataact ttacagtatt cgcaaattgg tttgaccgat
     8401 ggtcttactt tcatttctta tccctccaag tttttcgatt atttaaagcg gtaagtgata
     8461 cgtccacgtg tcaagtcata tggactcatt tcgacagtaa cacgatctcc cgctaaaata
     8521 cgaatatagt ttttacgaat tttaccagaa actgttgcta aaatctgatg tccattttca
     8581 agttcaaccg taaacattgc attcggcatt gtatcaacta ctttgccttc aacttcaatc
     8641 acatcgtctt ttgccacgca aaagcacctc cataaatttc gattcgatgc ctctagacac
     8701 agagacaaca attataagtc agactatttc agtataacat ttgtagcgga tttttgcaag
     8761 tgtgaaaaac gctttatttc aaatttgtca atactttttc aatatctgag aagacatcat
     8821 tgatatcttg attaccttcg atgtcatgaa ccaaaccttt ggcacggtag tgagcaatga
     8881 ttggttctcc ttgagcaata ttaacatcca aacgacgttt tactgtctca ggcttatcat
     8941 cttcacgttg gtagtaatct tcttctttat agtcaactgg tgggttaaag accttgtgga
     9001 aagtttctcc agttacgcgg tggatgatac ggccactcaa acgttccaaa aggctgtcag
     9061 ggttcacttc aatattgata acaccttcta gttcaatgcc aagttcattc aatgttttgt
     9121 ccaaggcatg agcttgttca attgtacgtg ggtaaccatc caataagaat cctgtttctt
     9181 taatatcatc ttgtgaaaga cgttctttta cgattccatt tgtaacttcg tcaggaacca
     9241 attcaccctt gtcaatatat gacttagcaa gaacacccat ttcagtttga tttgccattg
     9301 cagcgcggaa catatcacct gttgagatat gtgcaacatg gaattgttct acgatttttg
     9361 ctgcttgagt tcccttacct gcaccaggta agcccataat caaaagattc atgatttgat
     9421 ctccttattt tatttttaaa tagaagcaaa aagtcttttc acccctattt aaaaacaaaa
     9481 tagaagaggg gagatccaac tcttacaaat gtcagagttt aaacctccac tccctcagta
     9541 tttactgatt cagtaaatac ttttattctg ttctgtccat gaaaccaaca tacttacgtt
     9601 tcaataggta accttccaat tgcttgattc cttcgatacc tgtagagata atgatcaaga
     9661 gacttgttcc accaaaggca acaacatcag aaagaccaaa tacatcttta gctgcaatcg
     9721 gtaaaatgga aatcacacca aggaagaggg aaccaacagt tgcaagacga cgaagaagtt
     9781 tagacatata ttcttctgta cctttaccag gacgaactcc atggatatag gcaccactct
     9841 tttgtaggct ctctgctgct ttttcaggat taatctgtac aaacgtatag aagaatgtaa
     9901 agagaataat caacaaagca tacatggcaa taccagttgg agaagtagtt gccagcatct
     9961 cttgtgctac ccttacccaa gcccaatcat gacctgtggc actcaaaaac tgaagaatag
    10021 ccgcaggcgc tgcagtaatc gaactggcaa agataacagg gataactcca gcagggttta
    10081 cctttaacgg aaggtaagag ctagatggag caccttgtgc aaccttagta tattggattg
    10141 gaattttgta ttctgcttgt tgaacataag ttgtaaagta aataatcaac aatacagtaa
    10201 taatcaaaat gattacgaaa atgatagatg aagtgatacg gctacttggg acgttcacaa
    10261 agtagtccac atagatgccc tgaatcatct ctggaattga ggaaacaatc ccggcaaaga
    10321 taatcatgga aacaccgttt ccgtatccct tatctgtaat ttgctcaccc aaccaagtga
    10381 caatcatact accagctgtt aagatgatac caatcgtcag aaaaacttgt ggagttaaag
    10441 cagttttaat caattgagct ccagccaagg tattaaaacc agctgtaatc ccgatagatt
    10501 gcacaaaagc gagaactaga gcaatataac gagtagcttg attcaatttt cttcgaccta
    10561 cttccccttg tttaccccac tctacaaact tgggtaaaat atccatttgc aagagttgga
    10621 caacaataga agcggtgata tagggactaa ctcctagggc aaaaatcgaa aagtttttta
    10681 gggcattccc cgacaccaag ctcaacatgt ttaagaagga taatccactt aaagcattca
    10741 agctattggc attcacacca ggaactgtaa tgctagttcc gatacgaaag accaaaacga
    10801 taaaaattgt aaataaaatt tttgatcgaa cctgcttgac tttaagagct tctcttaata
    10861 atttaaaaaa cataggtcac ctctcttaga tgacttctac tgaaccacct ttagcagtga
    10921 tagcttcttc agctgattta gagaatttag ctgctttcac agtcaatttc ttagtcaact
    10981 caccgttacc aagaatttta atacctgact tttcagcttt aacaattcct gcttcgataa
    11041 gaacaactgg agttacttca gcaccatctt caaagacgtt caattggtca aggttcacaa
    11101 ttgcgtattc tttagcgttg atgttagtga atccacgttt tggaagacga cggaacaatg
    11161 gagtttgtcc accttcaaaa ccaaggcgaa ctccgccacc gctacgagct ttttgacctt
    11221 tttgaccacg accagatgtt ttaccgttac ctgatgaagt accacgacca acgcggttac
    11281 gtactttacg agaaccttct gcaggtttca attcatgaag tttcattttt attttctcct
    11341 cttttgtaaa atgctagcgc cgataaggga gaaaaggttg tctcccccat caactcgcct
    11401 atacgatgtc atcatagatg actatatcta gttttagggg atggtacagt gcacatcccc
    11461 taaaacttca ttagtttact tcttcaactg ttactaagtg agatactgct gtgatcatac
    11521 cacggatagc agcgttatct tctttaataa cagagctgtt caatttgcca agtccaagtg
    11581 ctacaacagt tttacgttgt gatggaatgc atccgattgg agacttagtc aaagtaattt
    11641 taatttgagc cattttatcc cctttcttat gccaaatcag aaactgaaat accacgaagg
    11701 gcagcaattt cttcagcgcg tttcaattgt ttcaaacctt caacagttgc acgaacaatg
    11761 ttgattggag tgttagaacc aagtgattta gatgtaatat ctgccacacc tgccaattcc
    11821 acaacggcac gaactgcacc accagcggca actccagaac cttctacagc aggtttcaac
    11881 aatactttag ctccaccgaa ttctgaaaga acttcgtgtg ggattgttgt tccaaccata
    11941 ggaacttcga tcaagttttt cttagcatca tctactgctt tacggattgc ttctggaact
    12001 tcttgagctt taccagtacc aaatcctacg cgaccattgt ggtcaccaac aacaacaaga
    12061 gctgcgaaac gaagacgacg tccaccttta acaacttttg taacacggtt gacagcaact
    12121 acgcgttctt ctaattcaac tgcattgtct ttaaatgcca ttttctagtg tcctcctatt
    12181 agaatttcaa tccgttttca cgagctgcat cagccaaagc tttcacacgt ccgtgatata
    12241 gatatccacc gcggtcgaac accacttctg aaataccttt agcgtttgca cgttctgcaa
    12301 cgagtttacc gacagcaacg gcttgttcag ttttagttcc ttttgaaact tctttatcaa
    12361 gagttgaagc acttgcgagc gttacacccg ctacgtcatc aatcacttga gcgtagatgc
    12421 ctgtattaga acggaatacg ttcaaacgtg ggcgatcagc agttccagag agttttccgc
    12481 gaacgcgacg gtggcgtttt tggcggagtt tgtttttatc tggttttgaa atcacagttt
    12541 tcacctcttt agttttaaat cgtgtgctat gcacaaagtt ggaaaatagg ttggtggttg
    12601 atgatcaacc actcaacatt atttacctgt tttaccttct ttacggcgaa cgaattcacc
    12661 aacgtaacgg atacctttac ctttatatgg ttctggtgaa cgaaggctac gtacgtaagc
    12721 agctgtttga ccaactactt cttttgaaat tccgctaaca acgattgttg ttgggtttgg
    12781 aagttcaaaa gtaattcctt ctggagcttc aacttcgtct ggatgagatt taccaacagc
    12841 caaaacaagt ttagatcctt gaagctgtgc acggtaacca accccacgca tttcaagttc
    12901 tttcttgaat ccttctgata caccaacaac catgttgttc aaaagggcac gagtagttcc
    12961 gtggatagtt ttcatttctt ttgaatcgtt tggacggtga agagttactt cagtaccttc
    13021 cacacggatt tcaatatctt ttgagaactc acgagtaagt tctcctttag gtccttttac
    13081 agttacaacg ttgtcattgt tagcgagttc aacaccagca ggcaacacga taactttatt
    13141 accaatacgt gacatgttta ttttctcctg ttaaattgtc aggccaaaac ggccagtttt
    13201 cacggggttc agatacttat ttagttcaag agctgaactg aacacgctct aagaactgtg
    13261 caaaatagat aagttggctt gtttgtaaac aaacttctcc aacttcctaa ttttgctttg
    13321 ttctttacga gctttgtatc ttgattttac caaacgtaag cgataacctc accaccaaca
    13381 ttcttttggc gtgcttcttt atcagtaagc aaaccttcag aagttgaaag gatggcaatt
    13441 ccaagtccgt taagaacttt tggaaggtct tcacgttttt tgtagacacg aagtcctggt
    13501 ttagaaacac gtttcaagtt agtgataact ttctcaccat ttggtccgta tttaagaaat
    13561 acacggatga cgccttgttt gtcatcttca atgatttcaa cgttttttac aaaaccttcg
    13621 cgtttaagga tttcagcaat cccttttttg atgtttgatg caggtacttc aagtacttcg
    13681 tgtttagctt ggttagcatt acgaatacga gttaggaagt ctgcgattgg gtcagtcata
    13741 accattttgt ttttctcctc ttactagtag tttgcaagtt gcacttgcta gttaatacgt
    13801 gatacaaaga gcgtaacagc acgagcaaaa atagacaatt taatgcagaa gcaatgctcc
    13861 aaagcaaatt ggtctttttt gccaaagctg tagctcgtgt tcaaattggc aagcccaatt
    13921 gaacccgggc taaactctgt gtgaaaaaga taaactttcc tagaaacttt agtttcttca
    13981 tcaagtttcc tatttttact tggagttttg acgctcttga tatcttaaat taccaagatg
    14041 cttttgttac accaggaatt tgacctttat gcgcaagttc gcgaaaagcg atacgactca
    14101 gaccaaattt gcggtaaact gaatgtgggc gccccgtaac tctacaacga ttatgtaaac
    14161 gagtcggtga ggcgttgcga ggtaatttag ataaaccttc gtagtcccct gccgccttta
    14221 atgcagcacg tttttcagca taacggtcaa caattttttg gcgtttagcc tctctagcta
    14281 ccattgattt tttagccatt agatttacct cctatattat tttgcaaaag gcattccaag
    14341 gcctgtaagc aatgcacgtg actcttcgtc agtgttagca gttgttacga taacgatgtc
    14401 aagaccacga gttttgtcaa cgtcatcgaa gttgatttct gggaagatta attgttcttt
    14461 cacaccaagt gtgtagttcc cacgtccatc aaatgatttt gttggaacac cgtgaaagtc
    14521 acgtacacgt ggaagtgaaa ctgataccaa tttatccaag aattcgtaca tacgttcacc
    14581 acgaagggta acttttgcac cgatcgcaac accttcacga agacggaagc cggcgattga
    14641 ttttttagct ttagtgataa gtggtttttg acctgagata agtgccaatt cttcagcagc
    14701 tttttcaagg cttttagcgt ttgatacagc ttcaccaaca cccatgttca aaacaatctt
    14761 atctacttta ggcacagcca tcactgatga gtagttgaat tgttctgtca aagcaggaac
    14821 tacttcatta agatattttt cttttaaacg atttgccatt atacttctcc tttccttcgt
    14881 gattaatcaa gcacttcgcc tgattttttg ttgtagcgaa cttttttacc gtctacaaat
    14941 ttgtatccaa cacgaccagc tacaccattt ttgtccaaaa cttgaacgtt tgatacgtgg
    15001 atagctgctt ctttctcgat gataccacct tgaggaagct cgttagttgg acgttggtgt
    15061 ttcttaacaa tgttaacacc ttcaacgata actttgttta cttttggaag ggcagtaagg
    15121 acaacagctt ctgttccctt gtctttacca gcgattacgc gaactttgtc gccttttttt
    15181 acaaacatta ggtttctcct tgatttttct tacgcccata agggcaccct agcttgaagc
    15241 taggggacta gtttgttcct aaaaattaaa gtacttctgg agcaagtgac acgatcttca
    15301 tgaagccacc ttcacgcaat tcacgtgcaa ctgggccaaa gatacgtgtt ccgcgaggag
    15361 ttttgtcttc acggatgata actgctgcgt tttcgtcaaa tttgatgtat gaaccatcag
    15421 cacgacgagc acctgattta gtacgaacga taactgcttt aacaacgtca ccttttttaa
    15481 ccgcaccacc aggagtagct tgttttacag atgccacgat aacatcaccg atgtttgcaa
    15541 atttacgtcc tgaaccacca agaactttga tagtcaagat ttcgcgagca ccgctgttgt
    15601 ctgcgacttt caaacgagtt tctgtttgaa tcatttcagt tttctccttt caggtttgat
    15661 tagatgatga ccgcttcttc aacaacttct acaagacgga aacgttttgt agctgaaagc
    15721 gggcgagttt ccatgatacg tacgatatcg ccttctttgg caacattgtt ttcatcatga
    15781 gctttgtatt ttttagagta gttaatacgt ttaccataga ctgggtggtt acgttttgtt
    15841 tcaactacaa ctgtgattgt cttgtccatt ttgtcagata caacacgtcc aacaagaact
    15901 ttacgattat tgcgttccat tgaaatttct ccttccctag tctattattt cgcttcagat
    15961 tgaactgttt tgatacgagc gatttgtttt ttaacttctt tcaagcgagc tgtttgttcc
    16021 aattgaccag tagcagcttg gaaacgaagt tcaaacaatt cttttttcaa ttcgttttcg
    16081 cgcttcgcga gttcttcttg agaaagacca cgaagttctt taacaaattc ttttacttca
    16141 ttaagtttca tgccttctcc ttattctgct tcacgtttta cgaatttaca tttaactggc
    16201 aatttgtggc tagcaagtcg aagcgcttca cgtgcaatct cttcagatac accagcgatt
    16261 tcgaacatca ctttaccacg tttaactggt gctacccaac cttcaggtgc ccctttacca
    16321 gatcccatac gcacaccgat agctttagca gtgtatgatt tgtgtgggaa gattttaatc
    16381 caaactttac caccacgttt catgtaacga gtcatggcga tacgagcagc ttcgatttgg
    16441 cggttagtga tccagtggct agttgtagct tgaagaccgt attcaccgaa tgctacttct
    16501 tttccacctt ttgcttcacc gcgcattttt ccacggaact cacgacggtg tttaacacgt
    16561 ttaggtacta acattggtta tttacctcct ttagtgtttt tacgagctgg aagaacttca
    16621 ccacggtaga tccatacttt aacaccaagt ttaccgtatg tagtatctgc ttcttcccaa
    16681 gcgtaatcga tatctgcacg aagtgtgtga agcggaacag ttccttcaga gtatccttca
    16741 gcacgggcga tatctgcacc gttcaaacga cctgatactt gagttttgat tcctttagct
    16801 ccagcacgca ttgcacgttg gattgcttgt ttttgtgcac gacggaaagc aacacgttgc
    16861 tccaattgac gagcaattcc ttcacctaca aggtgagcat ccaaatcagg ttgtttgatt
    16921 tcgatgatgt tgatgtgtac ttgttttcca gtcaatttgt taagttttgc acggagtgca
    16981 tcaacgttag caccaccttt accgataacc atacctggtt tagcagtgtg aagtgaaacg
    17041 ttaactttgt ttactgcgcg ttcgatttca atagttgaaa ctgctgcgtc agcaagttct
    17101 ttttgaacga atttacggat tgcaagatct tcatgaaggt aatccgcgta ttctttttca
    17161 gcataccatt tggcatccca atcacggatg atgccgacac gcataccaat tggatgtact
    17221 ttttgaccca cgattttacc tccttatttt tctgcaacag ctacagtgat gtgagctgta
    17281 cgtttgttga ttggtgaagc tgaacctttc gcacgtggac ggaaacgttt catagttggt
    17341 ccttcgtttg cgaatgcttc agatactacc aagttagctt tatccaaacc aaagttgttt
    17401 tcagcgttag ctacagctga gttcaaaact ttcaagatga tttcagcagc tttgtttgga
    17461 gtgaatgtca agattgcgat tgcatcggct acgcttttac cacggatgtt atcaagaaca
    17521 agacgtgatt tacgaggtga aacacgtact gtacgagcca ttgctttagc tgaagtaatt
    17581 tctgccattt atgttctcct tattttctac gtgttttctt gtcgtctgca gcgtgacctt
    17641 tgtaagtacg agttggtgca aattcaccaa gtttgtggcc taccatgtct tcttggatgt
    17701 aaacaggtac gtgtttacgt ccgtcataaa ctgcaatagt gtaaccaatg aaacttggga
    17761 agatcgttga tcgacgtgac caagttttaa taactttttt cttttcgtcg ttagcttgag
    17821 cttcaacttt tttcatcaaa tgctcatcga cgaaaggtcc ttttttaaga ctgcgtccca
    17881 tttttatatt ttctccttta aatgttgtac cacagcggct tgcactccta tggagcgcta
    17941 ccgagctggc ggatttacta gttgcttaag cgactagttt aatattattt ctcgttgcga
    18001 cgacgaacga taagtttgtc agatttcgct ttcttgttac gagttttaag accaagagca
    18061 ggtttgcccc atggagtaga tggtgcttta cgaccaactg gtgctttacc ttcaccacca
    18121 ccgtgtgggt gatcgttagg gttcattaca gaaccacgaa ctgttgggcg gatacctttc
    18181 caacggctac gtcctgcttt accaaggttt acaagtccat gttgttcgtt tccgacaaca
    18241 ccaactgtag cacggcaagt tccaagaatc atacgaactt cacctgattg aagacgaaca
    18301 agaacatatt taccttcaga acccaatact tgagcagatg caccagcagc acgtaccaat
    18361 tcaccaccac gacctggttt caactcgatg ttgtgaatca aagtaccaac tgggatgtta
    18421 gcaagtggaa gagcgtttcc gactttgata tctgcttctg gacctgaaac gatacgttga
    18481 cctacttcaa gaccttttgg agcgatgatg tatgctttca caccgtcagt gtagtgtaca
    18541 agagcgatgt ttgcagaacg gtttggatcg tactcgattg ttttaacaac tgcttcaacg
    18601 ttgtctttat tacgtttgaa gtcaaccaaa cggtagaaac gtttgtgtcc accaccttgg
    18661 tgacgaactg tgatacgacc gttgttgtta cgaccagcct tgctcttcaa tgcaacaagc
    18721 aatgattttt caggagtgct tgttgtgatt tcagcgaaat ccaaagaagt catattacgg
    18781 cgaccgtttg ttgttggttt ataaacacga attcccacga tatttcctcc ttagattatt
    18841 cagcttcagc agcaaacaac tcgattgctt tagaatcagc tgtaagtgtg atgatagctt
    18901 ttttagtttt gttagtaaaa ccagtgtaac gtccaacacg tttagctttt ggttttacgt
    18961 tgattgtgtt aacattggca actttaacac cttcgaaagc agcttcaaca gcttgcttga
    19021 tcaaaagttt gtgtgcacga gtgtcaactt caaatacata tttccctgct tcaagttgag
    19081 ccattgagct ttcagtgatg acaggttttt tgataacatc atacaaattc attatgcaag
    19141 aacctcctcg attttagaga tagctacttg tgtgacaaga agtttgtcgc tatttgcgat
    19201 gtcaagaaca cttgcagttg tagcagttgc aactttcacg tttggaaggt tacgagctga
    19261 aagagctgcg aattcatttc cttcttcaag gataacaaga actttagaat cgatgctcaa
    19321 tgctgcaaga acttttgcaa attcagcagt ttttggagct gtaaatgaaa gagcgtctac
    19381 agctacgaat ttgttttcag caactttttc agagtaaact gatttaagag ctaggcgacg
    19441 aactttttgt ggaagtttgt agccgtatga acgtggagtt ggtccgaaga caacaccacc
    19501 accacgccat tgtggtgagc ggatagaacc ttgacgagca cgtccagttc ctttttgacg
    19561 ccacggtttg cgtccaccac ctgatactgc agagcggttt ttaacagcgt gtgttccttg
    19621 acgaaggctt gcgcgttggc tgatgattac atcaaacaca actgattcat ttggttcgat
    19681 accaaatact gcatcgttaa gaacaacttg gccagcttct ttaccagttt ggtcaaataa
    19741 tgttacgttt gccattgtga ctgatttccc ctttctttat tatttaccag ctttaactgc
    19801 tgatttgata gtgataagag atttcttagc acctggtacg ttacctttga taaggataac
    19861 gttcttttct ggaacaactt gtacaacttc aagattttga attgttacgc ggtcaccacc
    19921 catacgtcct gcaaggtttt tacctttgaa tacgcggtta ggtgcaacag gccccataga
    19981 acctggacga cggtggtaac gagaaccgtg agccattggt ccacgtgatt gtccgtggcg
    20041 tttgataaca ccttggaaac ctttaccttt agaagtacca gttacatcaa caacatctcc
    20101 agctgcgaat gtttcaactg tgatttcagc gccaacttcc aagccttcaa cgtttttgaa
    20161 ttcacgaatg aagcgcttag gagccgtgtt agctttcgct acatgtcctt tagcaggttt
    20221 gttgctcaat acttcgcgtt tgtcatcgaa accaacttgg atagcgttgt atccgtctgt
    20281 ttcaacagtt ttaacttgaa gaacaacgtt tggagttgct tcaataactg ttacagggat
    20341 caattcgcca gcttcagtga agatttgagt cattcccact tttttcccta agattccttt
    20401 tgtcatgaga aaatagttcc ttttctatat tttttattca aaaagttttt aacgagcgtt
    20461 ttttatgctc aagtctaaga tacaaagggc gtcaaactca acttgaaaat aggaaactga
    20521 cgcagtgtct agcagacact aggaagttta tctttttcac accgagttta gcccgtgttc
    20581 aattagattg aaacaccagc ctctgctctt ttatatttta gattaaagtt tgatttctac
    20641 gtttacacca cttggaagat ccaatttcat caaggcatca actgtttttt gagttgggtt
    20701 aacgatatcg atcaaacgtt tgtgtgtacg catttcaaat tgttcgcgag agtctttgta
    20761 tttgtgagtc gcacgaatga ttgtgtagag gctacgttca gttggaagtg ggattggacc
    20821 cgcaacttgt gcacctgtac gagtagctga ttctacgatt tttgcagccg ctgtgtcaag
    20881 cgtacggtgt tcgtaagctt tcaaacggat acggattttt ttgtttgcca tctttttctc
    20941 cttttcgtct atttaagata ataggctagc tccacaagaa aaccgacacg gtgttgcgtg
    21001 gcaatgcaac cgagcgtgtc gcaacctctt gcatcaaaac taaggctgta atttacagca
    21061 ccataataga ataacacaaa tcccctgcga ttgcaaggga tttgttaggt tttttcgttt
    21121 ttttgggatg aaactgtttt cctacttttt ctttctaaaa ttagggattt tcttctatat
    21181 ttcttctata cataagttaa gtcctcttga catcaaattt atcttctgat tgatccacta
    21241 agatatccgc cttagactca gtttctttat agtatcgcag atactgctcc cgtctcatct
    21301 gatggctagc caatataaag gatgcatcac gatttctcac agtcgtatct ctagcaaggc
    21361 gtcgctttaa ttcggtctcc tcatccgtgt agaaacagat ggttttttca aatagttcct
    21421 tgggtagaaa gccaacagac atcccttcga caatcaaaat tggtttggtt ccagacaaga
    21481 cctcaccagc cttccaaggt tcttcaattg tcaagacatc catacccgcc tgcaaggcaa
    21541 ggatatctct ctgcaaactc tccagttcat gcgccactgg cagactggct gtcacctttt
    21601 gattcggcgc gtccttgggt actaccagat ggcgtcctga ggtgatataa ggatctgtct
    21661 ctagcaaatt tactgtagta gaatctaaag cttggtacaa ttcctgtgca aaggttgatt
    21721 tacctgaagc tccatgaccg tatattccca gtgtcctgac tttccccgtc tcgatctctg
    21781 agactagttg gtctactaag tcttttttct tcattctctc ttcacctgtt catagctttc
    21841 ttttccgtca ttgagcttgt cccaaatcac tacttgccca cttttgagcg atttttgcac
    21901 atcgataatt cgttggttag acgaacctcg aaactggagc ataagatttc tcttagttcg
    21961 atcatatctt ccatcgacaa gaatgtcaat cagtgacaag agttccagtt tatctggagt
    22021 ttccaacatc atttcttccc aagtgtagcc ggtccaggac caaatgtcct tgtctggcaa
    22081 ttcctttcgg atgcgtttaa ctagaggcaa gagaatgcca gtattaagaa aaggctcccc
    22141 tcccagcaaa gtcaagcctt gaacataggg ttgggcaagg tctgccataa tctgctcttc
    22201 taattctgct gtatagggaa tgccagcatt aaaagaccaa gtcgcaacat tataacatcc
    22261 ctcgcagtga aacatacagc ctgatacata gagagagtta cgcacgcctt cgccatcaac
    22321 aaagttaaag gccttgtagt caatgatacg accttgacta agttcctcgc ttttccattc
    22381 ttgtggtttt ggattattca ttcgctacct ctatccaata acgctcgact ccattgtgag
    22441 catcctcaaa tattccacca tttgctagaa tgactgctct gctagcagga ttattcacgc
    22501 tacaggtcac cagagctttc ttgatgttct tttccttagc aacttgcaag ccctgacgga
    22561 gagtctcttt tgcataaccc ttgccttttt cagatggacg aatggagtag ccaatgtggc
    22621 cagcataatt cagtaacctc tcattcagtc tcaatcgcag attcaaaaat cttagagcac
    22681 aacctgattc accaaacaat acgaactgta tcgatggaac gcgattttca ggcaagttta
    22741 ttcccatctc tttttgaata tttgtttcca accactcttc atacacaaag ttctctgtat
    22801 cccaaaatcc tccatcatgg gctgattggc tcttttcaaa ctctgtcatc atatctaaaa
    22861 cagctttctt atccgctaat cttggtctgc gtaattccat ccttacctcc cactatgaga
    22921 aaagcgagag ctggctctca cttttatttt ttcttgttct tgtttaaaaa ttgttctctg
    22981 aagctaagca agcgttcttt cttaccagct ccacctttag agtgcttctc gtgataacgg
    23041 gtcacttgtg cgcgcccctt atcgtctagt tgatattttc ccatttcatt tctttctaat
    23101 ttgttacttg atgcccagct attttaatcg ttgaaccatt catatgtttg acacgcgcag
    23161 cgatttcctt gtgacgcccg ttgaccatcg gtcttgcttg aggattacct aggtagccac
    23221 aagttcgctt caccacatct actgttttag ggtcgctatt gccacagttt ggacaagcaa
    23281 accctctctc agttggttca aaatcccctt caaagtcaca cttgtagcaa cggtcaatcg
    23341 gagtattggt gcctagatag cctacacggt cataagcata atcccagaca gcttccaagg
    23401 cctttggatt ttgctgaagg actggatact cacaataatg gatgaaacca cctgacgcac
    23461 ctgcttctgg atagactttc tcaaagtcca atttttcaaa cggtgttgga tttttacgaa
    23521 catcgtagtg gaaagagttg gtgtagtatt ccttgtctgt gatatcagga atagagccaa
    23581 acttgtctat atctagtcgg cagaaacggt ctgtcagact ttcggatggt gttgagtaga
    23641 tagagaaatg gtagccatat tggtctgacc actcttctac acggcgtttc atatcgtgaa
    23701 tgatgtctag cgtgaattcc ttagcatctg gattactttc ccagctgtta ccaaagaaaa
    23761 ctgtcgctac ttcatacaag ccgatatagc ccagcgaaac ggtcgcacga cgattcttaa
    23821 agagctggtc aacactttct tctttaccta gatgatggcc aaaagcaccg tactgataaa
    23881 ggataggagc attcgctggt gtcgcctctt tagtgcgttc gacacggtaa acaagagcat
    23941 cttctgcgat attcattcgc tcgttgaaga tttcccagaa cttattcata tcaccttcag
    24001 actcaagagc aatacgaggc agattaaccg tcacaacacc cagattcatg cgacctgaat
    24061 tgacttctac accattttca tccttccatc cttggaggaa agaacggcaa cccataggca
    24121 ccttgaagga tcctgtcaag tcaacaatct tatcgtaaga taaaacatct ggatacatcc
    24181 gtttggttgc acactcaaga gccaactgct tgatgtcgta gttgggagtt ccttcctcta
    24241 aattaaggcc tcttttcaga gtgaaaataa gtttaggaaa gatagccgta cggtgttctg
    24301 aaccaagacc cttgatgcga atgtttaaaa tagctttttg aatttctcgt tcaaaacgac
    24361 tggttcccag accaaaacct agcgaagtaa aaggtgtttg tccatttgaa gtgaagagag
    24421 tattgatttc atactcaaga gattgcatgg catcgtagat gtccttttgc gttttcttcc
    24481 aagcgtaatc ttcccatttt tcaggcaata cccactcttc tgcatctttg agatgttttt
    24541 gatagttctt ctctgcataa ggcgccaaaa tttcatcgat acggtcagct gaacagccac
    24601 cgtactggct agaagcaacg ttggcaatga tttgagaaat ctgtgctgtc gcagtctgga
    24661 tagacttagg actctctacc tctgcatttc caatcttaaa accattttcc aacataccct
    24721 taaaatcaat caaacagcag ttggtcatag gggtataggg actgtagtcc aaatcgtgat
    24781 agtggatatc ccccttttgg tgggcattgg ctacgtgctt aggaagcatt tgcagtccga
    24841 ttgatttccc aacaatccct gctgtcaaat cacgctgagt gttaaagaca tcactgtctt
    24901 tattagcatt ttcattgaca actgtctggt ctttgttgag aagtttatga atactaaagt
    24961 tgatatccgt cgcttttgag cgctcaaaat ccctctgtgt ccgataagta atatactcct
    25021 cagccagcgc atattctttg gcttcaagga gttcatgttc tacgatattt tgaatttcgt
    25081 aaatcttaat tccctgtgga aagcgactat gaatttctgt aataattcgc tcagtcagat
    25141 cattgaggcg tttttcaacc aggggtgtca catccataac cttgtcagcc gccttgtgga
    25201 gagccttgtc aatcttgtcc acatcaaata caacacgtct cccatctcgt ttctcgacga
    25261 agagagttgg caaaattgta attttttctt ctagtgcaat catatgcatg ctcttttcta
    25321 aaatgttctt tctaaaaagt attataccac tctaaaaaga aaaatcaata tcttgtgtta
    25381 aaaatcctaa aatttttaaa aatacacaag atattgattt ggttatttaa ttttatagac
    25441 tttaaactct gagtaatcag tctttacttg ttggtaattt tcttttaaga gtgtttccac
    25501 ttcagaccag actaccacct tgtcatttac cacaatcacc ttaggttgtt tttctttcaa
    25561 gtcattaagt aacttattac gattttcctc agttgctgta tagtgcaacg gggacaaaat
    25621 cgccgatggc gacaagcgct cactcttacg ataaagagtc gcagtatcat cccatgcata
    25681 gatagcatct tccgtatttg tctcctcttt taccaaatca gcaagacgat tacgttcttg
    25741 ataaggtact ggataaagga caaacttcat caaaaaagga acagacaaaa gatagacaaa
    25801 cccaaaaact agcagatata gatttccttt gaagaaacga ctccataggc taactggcgt
    25861 ttctcttcgg cttcttctag cactacgccc cctctgttga ctcttaatat tggttaccaa
    25921 taaaagtagg aaaacaggga agacgaccat caaataagaa gcgtgcagag gctcttgtga
    25981 aaagaaaagt aaaatcaaac ctaaaattac cacaagactt gcaggaaccg aaatgacgta
    26041 tagtttaaag ggtttggatt gaaagagacc taaaaagata caaaccacaa atcccaaacc
    26101 aagggacaac aagccataaa aaaacatatt ctctaaaatt ttagaagtaa aatcaaagcg
    26161 aatactgtca ataggataac gaataccact aatcgcatcc ccaaaacttc ctgttgcagc
    26221 actatagtag gcagttggat aaaagacaag tgaaaaacct aaagccactg caagaaactg
    26281 ataaaaccca tgcgcaaagc gtctatgccc aaggttaaag accaacaagc ctaaactcac
    26341 tacagcaata aacaggagcg atgataaggg agcaaagaaa aatccgcctg ccaaagctag
    26401 cccaatccgt acaaatccct tatcatggct tggattgctt aggtaagccg caactaaact
    26461 aaaggctgcg aataagaaag gaagcgctaa aagagtcgca tagcctccac caaaagcaag
    26521 acctgtaact agcatgtaaa aaatagtcac cagatgtcca gcttggtctc cttgctctgt
    26581 caaggtgtcc gctgatctaa aaaggaaaaa tcctcctgct accaaggcta accactcaaa
    26641 gatggcaaag aaaaatccgc cctgactcac gtaagtcagc aaataataaa gcaaaccttg
    26701 acttccataa tagtcgctgt aaatcttccc tgtctgatga agcgcccaac ctgcataaaa
    26761 atcctgcact tcttgtgcac tcattaagtc gagtaatagc ggtactccta gagttatccc
    26821 cgttacaagc gtactccata gtaaaatttt caccaaagga agacgacttg attcacgatg
    26881 atgcgattct tgttcgattt ggtattctag aggttcacga ttctccttat gaacttcttc
    26941 tactctacca tacacactca tatcgtttct cctgttcaat ttatctgtct tagtatatca
    27001 aaaagtccta ggattgtcag cttgcgatgg ctgtttgagt gattttttgc atagtttcac
    27061 aaaggtttca atttcccgaa agcggtgtaa atgtgtctgt ttgaactcaa tcatatcatt
    27121 cacttcttcc ttagttagca tgaattcccc tcctctttct ttctattcgt aaaagacaag
    27181 aaaaataggt ctggtttgta tcctaagttc tagatttctt ataagatagg ggcgaatagc
    27241 tgggcgattt cttttacaag cttttgatac cagctcgttt tgatagaatg aggatagact
    27301 tcctgagaat ctgcaaatat atttcgaaaa tctcgggcga tttcccttat agaaggagtt
    27361 ttatagagta agactgcatt ttcataatgg tgtaccaagc tccggtagtc gagattgatt
    27421 gtccccacca ccgcaaaatc ttcgtctacc aacatctgct tactatgaat aaaacctgga
    27481 ctatactcat aaatccgaac accagcagaa agaaggtcgg gataagctcc tctcgtgact
    27541 aactgaatga acttcttatc tggtatgtaa ggggtgataa ttcgaacatc gacccctctc
    27601 atagctgcat ttttgattgt ctctgttaaa tcataatcta taatcaaata aggtgtcgta
    27661 atatagaccg attctgttgc ttgattgatt aaactctgat aaactttttt ccctacctgc
    27721 gctcgaaaaa ttggcttggg tccacttccg tatggaatgg ttaaaccgtc actcgggata
    27781 gaatgatttt ctaaatgata ttgatcaaaa tcactaattt ctcctcgatt aatgtaccaa
    27841 gtggtcaaaa ataagcgtgt cagagctttt actgctagtc cgtctaagcg aattccacta
    27901 tccttccaat aaccaaatct ctcgacgtgg ttaatgtact catctgccag attgacccca
    27961 ccagtatagg ctatctgacc atcaacaatc aatatttttc tatgatctct gttattataa
    28021 gccactgtca aacgaggaat aactttattg aatttatggg cctcgatgcc cagctgacga
    28081 agtcgatgtg cataatctcc tgttaaagta gccatacagc cgatatcatc atatagcatc
    28141 ttaacctcta caccctgagc taccttttgc tctagtatat ctagtatgcg attccacatc
    28201 aaaccttctt ctataatgta atattccaag aaaataaatt tctcagcctt tttaagatct
    28261 tctaccatct ttttccacat agcttctccg ttaggaaaaa atgtagaggc agtttgatca
    28321 tagatgtcag cattggtatc catactcaat aaggacttga tgactccata agctgccttg
    28381 tcactttctt ttaatttctc ttttagtagc tggctatttg cttcttggaa atgcatagag
    28441 cctagcttct tcagttgttt gatttctttt ttggacaatc gcctttcacc aaacatcaga
    28501 tagagcaagg gaccaaatac tggcacaaag gctactaaca accaggttac cttattctca
    28561 ggagtcgtat tacggttgac tatcgaaatg attgtgatga tactcagtag aatgaggaca
    28621 gtaatccata aaatgggagc catgcgtcct aaataaagaa agagaccaaa aacaagacaa
    28681 agttcaagta gcataatcga aagactaaag ccatacttgg acatcaataa ttgaaatttc
    28741 ctatatttca tatcccctcc ttgatagttt gaatgctaaa aacaagtaat tgatactagt
    28801 ataacgcagg tattcggtag aaggcaagta tttatgatta ttttctaggt aaaagtaaaa
    28861 aggctgaaaa atccagccct ttctacttgc atagtcttat ttagttaaaa cgatttggtc
    28921 cgatgggtct ctatccatat catctacgac gatttgatta ccgtttacag tgtaaatttt
    28981 gacatcatcg ccaataatca tgcgttgatt tgctgaatca aatgtaacct gtttgacttt
    29041 ctgatccccg tcagattcga gctcagtcca tgtaccagtc gttccagtta ccactaaagt
    29101 gatacggtct ccgtcatcct gaccagtata agtcccatca atatcggtcg gttgagcaac
    29161 aggttggttt tgtactggag ccgcttgatt ttggttacct cctgcatttg cagtattggt
    29221 attctgttga gcaggctgtt ggacctgctg agcaacaggc tgttgaacct gttgagctct
    29281 ttgtgaacaa gctactaaga gactaaaact tgctagagaa gcaagagaaa gtgtgaatct
    29341 ttttaatttc atgatgaatt tcctttctgc taccaattta gagaaatttt ctctaaccag
    29401 caattcccct agtataacaa gttcaaaaaa gggggtcaat ttatctgctc acggtccagc
    29461 aggtagcccc gtacttctga gataaaatag agagaccctg taacgaacag caagtcttga
    29521 gcgtctgccc tttcttcgaa actgctgata aattctcggt aagagggaat tacatggtaa
    29581 cctgttacat ctgtctcgtc caaagaccct tgatagtcaa agccggtcat tttgagttcc
    29641 acttgaggta aattttcagt cagataaccc aacatccctt gataatcctt acgtttcaag
    29701 gatccaaaga ggatttgagg tcgatagcct tcctgctctt tttctttgat aaactcaacc
    29761 aagcaagtca aggcagggag gttatgagca ccatccaagt aaatctgtgg gcgaatacgc
    29821 tccaagcgac cagcccaatg ggtctgttcc aaggcctttc ttacagcctg ttcatcaata
    29881 gcttcctttc tttctcccat aaaaagaaga aaagtttgca acgccaaggc cgcattctcc
    29941 tgctgatagg ctccttctaa gcctatgttc agctgtgaaa gatttagtaa agagcttgaa
    30001 aaatcaccat tcagcactga aaaatcttga cctgcctgat aaaggtcaac agctaaagat
    30061 tcggcttttt tctgacagat aagcctagct tctggaggca attttgcaat cactgccttt
    30121 ttaccagcct tgaaaatacc agctttctgc tctgcaattg cttctagact atcacccaag
    30181 gtttcttgat ggtcaagccc aatggaggtg atgacagcaa gctctccagt taccacattg
    30241 gtcgtgtcaa gtaagccacc aattcccact tctaataaaa ccaaatccac ctcctgctcc
    30301 ctaaaataaa gaaaagcaac caaggtcagc aattcaaaaa aggacaactg gtcaggagtt
    30361 tgcagaagcg ttttctccat ctccttgacc tgatcagcca aacggataaa gtctgcgtct
    30421 gctataggtt gcccattaat gcaaattcgg tcattgatgg atacgatatg aggggaggta
    30481 aaggtcgcaa cttttttgcc atgccccata aataattccc tcataaaagc aatggtggat
    30541 cctttcccat tagtccctgt tacgtggata atagggtaag actgctcagg atttcctaac
    30601 aaatccaccg cttgctgtat tcggcccaaa cctgatcgaa aattcaaacc aatccgacta
    30661 tgaagccatt cttctacttc aaacatacac atctccttga caaaagtcca atcaattatc
    30721 gcattaaagt atagttacta ataaaaacaa ggccaggatt ttcgtcccga cctcttacct
    30781 ggttagctaa taactagcta ctatgaatgt gaacgtgggc taaaaacatc cactgaatgt
    30841 tccaactctt ctcaatttct aggagttggg gtaaaaatgt tcactggacg ttccaactct
    30901 tccccatttc tgggagttgg gctgatacag tctcccagac tacctgcgtt tctaatttct
    30961 agcgcagggc taaaaacgtc ccccggacgt ttttactcct ccataaagct gttgaagact
    31021 tcttcaatca tgttccattc gtcttctgag tcttctggga ttggttgcaa ttcgccttct
    31081 gttccatctt cgttttcgat gaatgagtaa gcttggattt caacttgtcc gtcttcgtct
    31141 tcttctgcgt taactggtac tagaagaaca tagtttttac caaattcttc ttttccatca
    31201 attgtcaaaa ggatttcaaa caaggtttca tttccttgct catctactag tgtgattagt
    31261 tcacgttctt cgtggtcgtg gttatgatcg tgtgacatag cctctccttt atattaaaat
    31321 tttctatcta aataattttg taaaatcagc tgagctgcta acttatcaat gactttcttg
    31381 cgcttattgc gactgatatc tgcttgttca atcaacatgc gctcagcagc cactgttgtc
    31441 aagcgttcat cctgatagtc tactggtaaa ccaaaaaact cttctagctt tgctccgtag
    31501 gcttgactag cttctacgcg cggtccactt gtattgttca tgtttttagg caagcccact
    31561 acaaatcgtt ccaccttgta agtatcaacc aattccttaa cgcggtcaaa accaaattgg
    31621 ccttgttctt catttatctg gatgatttca agcccttgag ctgtaaaacc aagcggatcg
    31681 ctaatcgcca cccctaccgt ttttgaaccg acgtccaatc ccataattct cataggttat
    31741 agatcgactc cttgtccttt gaggtagtag cgaaccaatt cctcaacgat ttcatcacgc
    31801 tcatacttac ggatttgatt tcgtgcatta ttataacgag gaacgtaggc agggtctcca
    31861 ctcaatacgt aacctacgat ttggttaatt gggttgtaac ccttatcgtt caacgaagca
    31921 taaacgtctg tcaaagtttc gctaatttct tttttattgg aatcgtccaa tttaaaacgt
    31981 actgtttctt cagtaaatcc cattctaaca ccctctttcc ttagaatagt accattatag
    32041 cataattcct taccttctac aattcaggca gtctatttat ttggattttc tactgttctg
    32101 tcgcaccatt tgccaatcta tctgaaatat atttgcttgg ttcatttttc aaaagatttt
    32161 ccaaaccaat attcttcaga tgttccaact gggaagcctt cttgacatcc agaacttgaa
    32221 aatcaaaact agtcgttgtt tgaagttccg ttgcgctcaa tagttttgtt tcaagtttga
    32281 aacctaccaa tttacgagct tcaatgatag acttatcctt ctcctccgct tcaagaagag
    32341 ctttttgagt ttcttccact ccctgttggt caatataagt cttcaactca tcagaaactg
    32401 gacgtttttg ttgaatcgtt aagctcaaaa aagtcttgtc cttgtatgta atggtttgag
    32461 tttgctggct accatcatct gacttgggca agaccaaagt cttggttaca actgtattct
    32521 tctcggcatt ttcaataact ggcaatgccg actgaagcgt atccttttct gtttttgtag
    32581 ctggtccagt ttcttttttc tgtccgcaac caaccaggac aaaaaggaaa gctagactaa
    32641 caagaactat ttttttcatt tctttcttct ttctttttga aattaaaata gaataagact
    32701 gggaattgct cccagccttg atgtttatag agctgcacgt aaacgtgctt ctgcattttc
    32761 tacattacgg acagagcgtg gtaggaaggc acgaatatcg tcttccttgt agccaacttg
    32821 caggcgtttt tcatctacaa ggattgggct ctttaaaatt ctcggtgttt ccataatcag
    32881 attgagaact tcattgacac tcaaatcttc aatatccact ccaagggctt tggcatagcg
    32941 atttttagac gaaacgatgc tggctattcc gttatctgtt ttggttagaa tatccagtaa
    33001 ttcttctctc gtaattcctt ctttaccaag gttttgttct ttataactta actggtgggc
    33061 attgagccag gtttttgctt ttttacagct agtacaactt gagactgtat aaattttaat
    33121 catgtaccta cccctttcgc tacatgttac tatcagttta gtctattata ccataaaaaa
    33181 catccgactt gcgacctatt tttaa
//
LOCUS       DBERMR010000022        33086 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.022, whole genome shotgun sequence.
ACCESSION   DBERMR010000022 DBERMR010000000
VERSION     DBERMR010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 33086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33086
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.022"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(126..1106)
                     /locus_tag="ACL8A1_001100"
     CDS             complement(126..1106)
                     /locus_tag="ACL8A1_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HGS1201885.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            complement(1423..3438)
                     /gene="recG"
                     /locus_tag="ACL8A1_001101"
     CDS             complement(1423..3438)
                     /gene="recG"
                     /locus_tag="ACL8A1_001101"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HGS1201886.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKYLAEYKQALRRIKFEELFYFQMQ
                     LQMLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            complement(3457..4560)
                     /gene="alr"
                     /locus_tag="ACL8A1_001102"
     CDS             complement(3457..4560)
                     /gene="alr"
                     /locus_tag="ACL8A1_001102"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HGS1201887.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVCVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKLYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            complement(4550..4912)
                     /gene="acpS"
                     /locus_tag="ACL8A1_001103"
     CDS             complement(4550..4912)
                     /gene="acpS"
                     /locus_tag="ACL8A1_001103"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HGS1201888.1"
                     /translation="MIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLKGR
                     RQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLSIS
                     HTDQFVTASVILEENHES"
     gene            complement(4957..5988)
                     /locus_tag="ACL8A1_001104"
     CDS             complement(4957..5988)
                     /locus_tag="ACL8A1_001104"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HGS1201889.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            complement(5990..7021)
                     /locus_tag="ACL8A1_001105"
     CDS             complement(5990..7021)
                     /locus_tag="ACL8A1_001105"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HGS1201890.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            complement(7102..9615)
                     /gene="secA"
                     /locus_tag="ACL8A1_001106"
     CDS             complement(7102..9615)
                     /gene="secA"
                     /locus_tag="ACL8A1_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HGS1201891.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYTQRYDVITADRDL
                     APEIQAMIKRTIGRVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            complement(9761..10048)
                     /locus_tag="ACL8A1_001107"
     CDS             complement(9761..10048)
                     /locus_tag="ACL8A1_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201892.1"
                     /translation="MLNLKSFITRYSKVYIGLVLLIWIAFFFLPWGSPILGGKIDLFS
                     IQKVLLVFGILSILMALLSKKVSLFVFGLICCLSLWINLFILFAILPILGN"
     gene            complement(10042..10617)
                     /locus_tag="ACL8A1_001108"
     CDS             complement(10042..10617)
                     /locus_tag="ACL8A1_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HGS1201893.1"
                     /translation="MKTIVKICLVALSAFLLLFAGKNVFADSNSHDNLNSLEVSDRSF
                     RNGELLKSYLVKDKDRIYFDYDKAVQDKQAKDVIDMGLLVETISEDYSSNTFTPAKYF
                     KAGLPVHGNYCGPGYNGEGFTLPVVDVLDQGCQNHDRCYKWGAGIGANCECNRQLVDF
                     IKVNRRWIPESALWVADAIRVYFETIGAIGC"
     gene            11079..11930
                     /locus_tag="ACL8A1_001109"
                     /note="rgg1518"
     CDS             11079..11930
                     /locus_tag="ACL8A1_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator
                     Rgg1518"
                     /protein_id="HGS1201894.1"
                     /translation="MRWDYGQIFKEIRKSKGLTQQDVCGQVIHRTTLTNIEHGKVIPS
                     FENMVFLLEQIDMSLAEFKYIWNEYHPSKRRDIIVESQNPSTFQDTRKMVELTEKCQK
                     YLKTHHDVPIQNIYRHTKIVTELRTKGFKNNHVLKDLSEEIWDYLEPMDTWYISDLKL
                     LGTILFFFPSENLPLLIDRIMKTIEKYKYFRETKVFLSSFLANLSTVYFQHHLFKECE
                     TITLQLLVLAEELKIYDILGFSQVRLGILQHNSDLIDKGITLLRLTKEEALVEILEKE
                     INDFSNL"
     gene            complement(12152..12502)
                     /locus_tag="ACL8A1_001110"
     CDS             complement(12152..12502)
                     /locus_tag="ACL8A1_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201895.1"
                     /translation="MEHLFKMIILLPCFYFFSWIEKANRDSKIFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLLFNIVLRNVADIKSWGVWLLLLLIAFASDWLAYVFFKKMFRLRQE
                     LGKSKGVHKGRCRN"
     gene            complement(12642..12977)
                     /locus_tag="ACL8A1_001111"
     CDS             complement(12642..12977)
                     /locus_tag="ACL8A1_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201896.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(13068..14378)
                     /gene="der"
                     /locus_tag="ACL8A1_001112"
     CDS             complement(13068..14378)
                     /gene="der"
                     /locus_tag="ACL8A1_001112"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HGS1201897.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(14392..15105)
                     /locus_tag="ACL8A1_001113"
     CDS             complement(14392..15105)
                     /locus_tag="ACL8A1_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HGS1201898.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     ALLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYPVFGMAL
                     GVPNQTHDVKPRLPLDNVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            complement(15102..15998)
                     /gene="dnaI"
                     /locus_tag="ACL8A1_001114"
     CDS             complement(15102..15998)
                     /gene="dnaI"
                     /locus_tag="ACL8A1_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HGS1201899.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTVWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(15999..17168)
                     /locus_tag="ACL8A1_001115"
     CDS             complement(15999..17168)
                     /locus_tag="ACL8A1_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HGS1201900.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLGHPVYRRLLEKKIGDVAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKEATIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEEAVLRIRDR
                     GQKAKTQKQNQTAPEKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(17169..17642)
                     /gene="nrdR"
                     /locus_tag="ACL8A1_001116"
     CDS             complement(17169..17642)
                     /gene="nrdR"
                     /locus_tag="ACL8A1_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HGS1201901.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            17782..18147
                     /locus_tag="ACL8A1_001117"
     CDS             17782..18147
                     /locus_tag="ACL8A1_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGS1201902.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            18152..18847
                     /locus_tag="ACL8A1_001118"
     CDS             18152..18847
                     /locus_tag="ACL8A1_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1201903.1"
                     /translation="MSLLVFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            18864..19631
                     /locus_tag="ACL8A1_001119"
     CDS             18864..19631
                     /locus_tag="ACL8A1_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201904.1"
                     /translation="MFWNLVRYEFKNVNKWYLALYAAVLVLSALVGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTSVLLLCIYIILLSTNSNEVQLSTLLSFVGEHFTGFSLTVLSFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVVGFIEVFFNTSTNFYINSLAGF
                     NDNFYMGASIGIVEELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(20044..21243)
                     /locus_tag="ACL8A1_001120"
     CDS             complement(20044..21243)
                     /locus_tag="ACL8A1_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1201905.1"
                     /translation="MRNMWVVIKETYLRHVKSWSFFFMVISPFLFLGISVGIGHIQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILVHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGSFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAEGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(21240..22133)
                     /locus_tag="ACL8A1_001121"
     CDS             complement(21240..22133)
                     /locus_tag="ACL8A1_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1201906.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            22255..22446
                     /locus_tag="ACL8A1_001122"
     CDS             22255..22446
                     /locus_tag="ACL8A1_001122"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201907.1"
                     /translation="MKSKEQTRKLAVGCSKYSFEVADKTDEVSSKHCFEVVDRTDEVS
                     NYIYGKAKLTWFEEIFEEY"
     gene            22814..23027
                     /locus_tag="ACL8A1_001123"
                     /pseudo
     CDS             22814..23027
                     /locus_tag="ACL8A1_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            23442..24059
                     /locus_tag="ACL8A1_001124"
     CDS             23442..24059
                     /locus_tag="ACL8A1_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HGS1201908.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(24379..25266)
                     /gene="scrK"
                     /locus_tag="ACL8A1_001125"
     CDS             complement(24379..25266)
                     /gene="scrK"
                     /locus_tag="ACL8A1_001125"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HGS1201909.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEMVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(25415..27298)
                     /locus_tag="ACL8A1_001126"
     CDS             complement(25415..27298)
                     /locus_tag="ACL8A1_001126"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HGS1201910.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEAAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYALADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTADYASVAPVATGSVSKGDAVIE
                     VKI"
     gene            complement(27425..27622)
                     /locus_tag="ACL8A1_001127"
     CDS             complement(27425..27622)
                     /locus_tag="ACL8A1_001127"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201911.1"
                     /translation="MQAFYVKKEEKLSNDYHKINTGNQLNFYENVKDNEIKDFLTKVS
                     NLFFKEFLMKQSKTINLKLAH"
     gene            27675..29129
                     /locus_tag="ACL8A1_001128"
     CDS             27675..29129
                     /locus_tag="ACL8A1_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HGS1201912.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            29110..30075
                     /locus_tag="ACL8A1_001129"
     CDS             29110..30075
                     /locus_tag="ACL8A1_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGS1201913.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(30110..31384)
                     /locus_tag="ACL8A1_001130"
     CDS             complement(30110..31384)
                     /locus_tag="ACL8A1_001130"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HGS1201914.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(31384..32556)
                     /locus_tag="ACL8A1_001131"
     CDS             complement(31384..32556)
                     /locus_tag="ACL8A1_001131"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HGS1201915.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            complement(32687..32884)
                     /locus_tag="ACL8A1_001132"
     CDS             complement(32687..32884)
                     /locus_tag="ACL8A1_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201916.1"
                     /translation="MIAEKVSKTLDKTFDKDVREIPTSQFYQKFVDEMGRTYSGNLIL
                     QELITVNGAYKATYIGELSSN"
     gene            complement(33003..>33086)
                     /locus_tag="ACL8A1_001133"
     CDS             complement(33003..>33086)
                     /locus_tag="ACL8A1_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HGS1201917.1"
                     /translation="TTYRNHRKRFGLRMNLSAGIINHELGF"
BASE COUNT         9641 a         7431 c         6222 g         9792 t
ORIGIN      
        1 atttgccctg ttcttgtttc attttactat atttccatga taaaacgcat agtatcaagt
       61 tttcttaagc ccctgatact atgcgtgttt gtaattttta agattttgtg cttagagtcg
      121 actccttatt ttagatattt aaaaggaatc tcacttccac agagccagtt gtagacttgg
      181 tcattgacaa atacattcat ggcttcgtga gcatactcag gcatgatgcg ataggtttta
      241 tcgcaggtca gacgattata aatcgcaaac tgggtaatgg gatagcaaac atcgtcgtcc
      301 aagcccgtaa tcatcttaac ctcaccttgg atacgatggg caagattttt gacatcgata
      361 taggcaaggg tcgccatgat ttcctcctct gtttcatgga aggggtcgtg aaacttgaaa
      421 taacggaaaa gttcgtcgta agcctcgcta gtattaccaa tctcaatcac ccgtctgaag
      481 tctgacaaga agggataaat ggcaactgtt ttctgaattc gaggattgag cgctgctgca
      541 actagagcta gagcccctcc ttgtgaggca ccatagctag aaagacgctt ctcatcaacc
      601 tgagacagac tagcaacaat ttcgaccaac tggtaaatat ccagataaac atccttataa
      661 aagaggtggt cccgaccttc catagcacca cggataatat gccccttcac ggtatttcct
      721 aaaggagaac gcaagccgtc ttgtgagtaa cctgactggc cccgcacatc catggaaaca
      781 acaccgtaac cagctacggt gaagcccagc atgtcggccc agtcccagcc acgtcccata
      841 taaccatgaa aatggaagat tagtggaacc ttctcctcac tctttggaag aacaatgcgt
      901 gcatagacct ttccttcctt gcttccttca aatgttaact catagcactt gacttgagga
      961 atgtggaaat ctctttcctc caagtggtag gatggaagcg tggaaacatt tttcacttcc
     1021 ccatcccaga aagcatcaaa gtcttccgga acctcatccc ttcctctata ggtcttaatt
     1081 tcttctagca aagctggatt tttcatagca tgctcctttt attttgtaat cgttaccaca
     1141 actgtagcat atctatgtaa tcaatgcaag aaagaagggt aaatagaaag tgaatttaga
     1201 ttctttactt ttaggatgat gggctgaaag tagttttaga aacgaaaaaa gagcatttcg
     1261 ctctctcttc tgtttatact agttttttgc ttccttcttt tggaaagtgg tttggtaatt
     1321 tactttaatc gttactgtca tatgagagtc ctcgtttctt tttgatgact ctagtataag
     1381 agccaaacct aaaaactagc ttaagttttc cttagagaaa gcttaatcca gatgttcttt
     1441 cttttccaga tgaagggcaa tcatgcgcca ctctggatct tcttgccaag cttctataga
     1501 actaatgtag ctagcaacct ttcttgcttc ttctaaaatc ggaaaatctt cgataatatc
     1561 agccacttgg aactctggaa gtcctgactg tctggttcca aaaatctcac cagaaccacg
     1621 cattttcaaa tcttcctccg caaggacaaa tccattggtc gtttctgtca tgatgcgcat
     1681 gcggtctttc ccagaatccg tcttgggatt ggcaacgaga acagcgtagg actgcttgtc
     1741 cccccgaccg acacgacctc taagctggtg aagttgactg agaccgaagc gatcggcatc
     1801 catgataatc atgacagtcg cattgggaac gttgacccca acctcaataa ccgtcgtcga
     1861 aaccagaata tccgtctttc tctccttgaa atcctgcatg atctggtctt tttcgtcact
     1921 cttcatccta ccatgtagaa gagccacctc tgccttgcct gcaaaatgag tcgtcaactc
     1981 ctctgataag gcaatggcat ttttcaaatc tagagcttct gattcttcaa tcaaaggaga
     2041 gatgacatag gcttgggaac ctttttgaat ttccccctct aaccaagtca agacctgagg
     2101 tagttgctca tgtttgatcc agcgcgtcac aataggcttc cgacctgctg gcatctggtc
     2161 gataatggaa acatccatat ctccaaaggc tgtgatggca agcgtccgtg gaatgggagt
     2221 cgccgtcatc atgaggacat ctggattgtc acctttttcc cgtaaaatac gcctttgccc
     2281 tacaccaaaa cggtgctgct catcgataat aatcaaacca agacgagcat actccacccc
     2341 atcttgtatc agagcgtgag ttcctataat caaatcagcc tcacccttgg caatggtctc
     2401 caagacttct ctcttttctg cagctttcaa ggaacctgtc aagagagcca gtttcaaatt
     2461 gggaaaaagg ttctgtaaac tctcaaagtg ttgctctgcg aggatttctg ttggtaccat
     2521 tagggcagcc tgataacctg ctgtcactgc cgcaaacatg gccaagccag cgactaccgt
     2581 ttttccactc cccacatccc cttgtaggag acgattcatg tggtggtcgg acttcatatc
     2641 agttaaaatt tcctgcaaac tcttttcctg agcttgggtc agggcaaaag gaagacttac
     2701 tttaactgct gtcacttttt cctgagacca attcagaacc agaccacttc cctgaactct
     2761 attttcagac ttgagcatct gcagctgcat ttggaaataa aagagttcct caaactttat
     2821 acggcgaaga gcctgcttgt attctgccaa atactttgga aaatgcatag cacggactgc
     2881 ctgacaacgg gacatgagtt tgtatttgtc tagtaaagac tggggcagat tttcttctat
     2941 caagaggtcc agtccctgat caaaagccgt cttgatgacc ttgaccagac tggcctgact
     3001 gattccctga gccagacgat agacaggctg gaggtcatct tctacctgag ccagaacctt
     3061 catcccagtc agactagcct tagcgcggtc ccattttcca aagacagcaa gggttgctcc
     3121 caactctatt ttatcagcca gatagggctg gttaaagaaa ttcaccgcaa aaacgacctc
     3181 tccctgcttg agactaaaac gcaggcgatt gcgcttgaaa ccataatact ggacactagc
     3241 aggagtcact acctgaccag aaagaactgc cttctcaccg tcttccagct ccagcacctg
     3301 cttggttttg aagtcttcat aacggaaagg aaagtagagc aagagatctt gcaagttttc
     3361 aattcctagt ttggcgtatt tttctgctga ctttggtccc acaccaggca agacatgcaa
     3421 gggttgatgt agattcatgc tccactcctt tcttttctaa taatattctc tcggaatacg
     3481 gtcgctgagg aggcaaacca cctcatagtt aatggttacg cggtaggtcg ctacctgagt
     3541 tgcagtgatt tccttatccc cattggagcc aatcaaggtt accttggttc ctagcggata
     3601 aagcttaggc aatcgaatag tgatttggtc catcgaaacc ctgccgacaa ttgggcaagc
     3661 ttggccatct accaagacag agaaattttg catgtctctt gtccatccat ctgcataccc
     3721 gattggcacg gtcgcgatga cttgctcgct atccgcttga taagttgctc catagcccat
     3781 gcaagctcca gctggaactg tcttgacatg aaccagagca gactccaagg tcaaggccgg
     3841 tatcaaatca taaggcaaat ccaagaccgc tccacttgga ttgaggccat acatggcatc
     3901 tcccatacga accgcattga aaatagtctc tacatgccaa agagtcgttg cagaattgct
     3961 agcatgaacc agctctggaa cttccttcat actagctaaa atagttttaa accgttctaa
     4021 ctgggcatta aaatagtcat ctgattcctc atcagcagta gcaaagtggg taaagattcc
     4081 ttcaacacaa acaccgtgtt gttggagcaa atcttgagcc tgctcaacct cacttgcctc
     4141 tctaaaacca atccgtccca tccctgaatc aatcttgagg tggactgtca atccagttag
     4201 gtccacttcc ttatctaaga gtgcttgaat ccactccagt ccagccactg tcaaggtgaa
     4261 gtcatattct ttagctagag caacagcttc gatttcagaa actcctaaaa tgaggattgg
     4321 cttgctgagt ccagcttgtc tgagttcaat ggcttcatcg atattggaaa cgcaaaagcc
     4381 atcaacatca tcttgaattg ccttggcaac ggcaacagct ccatgaccat aagcattggc
     4441 cttaaccaca gccaacttga gcgttccttg agggatatga gcccccattt gctgaatatt
     4501 ttgtcgaata gctcccagat gaatcagagc cttggttggt ctatgtggac tagctttcat
     4561 gattttcctc caaaatgaca ctggctgtca caaactgatc ggtgtggctg atagacagcc
     4621 aaatctttcc tgaaaatggt gcctgactaa aataaggcgc cccacgttca ttgttcaaga
     4681 cttccaaatc ctgaaaaccg agcttgctaa tgcccgttcc catagccttg gaaaaggcct
     4741 ccttagccga ccagcgacca gctaaatatt ctatttgcct gcgtcctttg agactggtga
     4801 agcgctccat ttcctgagcg gtcagtacac gcttagcaaa tccttcatgt cgtgtaactg
     4861 cgctttctat cgaagccaat tcttcgatgt caattccgtg tccaactatc attctcatca
     4921 aaaggagttg agattcccca actccatcct tttcacttat tttgtcaagg taacataaat
     4981 ctcttctacc aaggcctctg tattttccca acctaggcaa gggtcagtaa tagagcaacc
     5041 aaagatctct ggttggtttt gacgaccatc tgctaggtaa gattcaatca taaatcctcg
     5101 aaccgtcttt ttaattttct cattccaatc acgattctgc aaggtctggc gaacaattcg
     5161 aatctgctcc atatattgct tgcctgagtt atcatggttg gtgtcaatga ggataaaagg
     5221 attttcaagt cccatggttt catagcgttc aatggcttgg agtagatttt catagtagta
     5281 attcggcata taattgccat actcgttgac tgctccacgg aggataacat gggccaaagg
     5341 attacctgat gtctcaactt cctgcccatg ataaaggaag gtttgcttgt tttgagcagc
     5401 atagatggcg ttaaacataa cacccaaatt tcctgaggtt ggatttttca tccctactgg
     5461 tgcatcaatc ccagaagcca caaagcggtg ctcttggtct tccacagaac gagctccaac
     5521 ggcatggtag ctgaccaagt catccaccaa gatcagattt gacggataaa gcatctcatc
     5581 tgccgttgtc aaaccagtct ctgtaatcac gcggtagtgc aactggcgca cagcctgcaa
     5641 gccattaatc aggcttggag ccttagaagt atctggctgg tgaactaatc ctttatagcc
     5701 gtctccattg gtacgaggct tagcagtata cacgcgcatg accatgaaaa tcttatccgc
     5761 taccttcttt tgcaaggcag ataaacggcg agcatattcc aagaccgcct cttcattatc
     5821 agaagagcaa ggaccaatca ccaagagaat acggtcatct tcccctgaaa taatatctgc
     5881 caattccctg tcacggcttt ccttgagtct caaggcttcc gcagacaatt gggtttcagc
     5941 cttgatgact tccatatcga tttcttgacc tttttcaata aatgccatct tattctccta
     6001 acgttttgta aatttctctg acaagagctt ctgtgttatc ccaacccagg caagggtctg
     6061 tgatagactt accaaatact tctggctcat tttgtcgacc atcttccaga taagactcaa
     6121 tcataaaacc acgaacgaac tgcttaattt tttcattcca agcacggtta atcaaggtct
     6181 ggcggacaat tcggatctgt tcaatatact gcttaccaga attgtcatga ttggtatcaa
     6241 tgatgataaa aggattttcc aagcccattt tctcatactg ggcaatggta tcaattaaat
     6301 tgtcataata gtagttggga atattttttc catactcatt aagagcacca cgaagaatag
     6361 cgtgtgaaag cgggttccca gttgtttcta cttcttttcc taagaaaagg aaactttgtt
     6421 tgttttgagc agcataaatc ccattaaaca tgacattgag atttccagag gttggatttt
     6481 taaaaccagt agaaaatcct gccccacttg ccacaaagcg gtgttgctgg tcttcaactg
     6541 aacgggcacc aactgccatg taagaaatca aatcatctac aagcggaagg ttttcaggat
     6601 aaagcatttc atcagcagtt gtcatccctg tttctgtgat gacacgatag tgaagatggc
     6661 gaacggcttt gattccattg ataagactag gcgcttctgt cgcgttaggc tggtgaatca
     6721 agcccttata gccatctccg ttggtacggg gtttggcagt ataaacacgc ataaccataa
     6781 agatacgatc tgccacttct tcttgtagga ctgccaaacg cttagcgtat tcaaggacag
     6841 cttcttcgtt gtcagatgag catggcccga ttaccaagag aattcgctgg tcttctccac
     6901 gtataatggc ttctagctct tgatctcgct gtgatttcct ctccaaagcc tgaccttcta
     6961 attttgacaa ggcacgaact tcttctatat ttattttaga gctttttgct gtaaatacca
     7021 taactcatct ccttataaag caaacggata cccagtaaaa atttactttt cacaagatat
     7081 ccgcctctaa actatctcat tttattgtct tttaccgtga cagtttttaa atttcttacc
     7141 agaaccacat gggcaaagtt cattgcgtcc aatctggctc aaatccaaat cttctggcat
     7201 acttgcttgg tgagcagcga tattgcgagt cgctgttgta ctgatatgac gttctgcctg
     7261 tggtctttct tgttcatgaa tttgtgcttt catcatcaag cgtgtcacat caaactcaat
     7321 cgaaccaatc atatcattaa acatacggaa accttctgcc tgatactcaa caacagggtt
     7381 gttctgagca tagccacgaa gtccaaccgc gttacgcaat tgatcaaggg catcgatatg
     7441 atctgtccac ttgttatcca ccactcgtag aatcaaaact ttttggaatt ctttaactgc
     7501 ttcttcatcg cgtagttttg aaacctgact atcgtaaacc ttcaaggcac gttggaaaag
     7561 ctcttccttg atggccttat cagacaagcc tgacaagtct tccatcgtaa tagaatcttc
     7621 aggaagcaag ttatacttag cgaagttcaa aattgcctct agtttctcat cttgtttagc
     7681 acgcgcatga ccgtcaacga cacgaccaat cgtgcgtttg atcattgcct gaatttcagg
     7741 tgccaagtca cggtctgcag taatgacatc ataacgttga gtatagataa tctcacgttg
     7801 ttcacgcatg acatcgtcgt attgaaggac ttgtttacgg gtatcgtagt tatttccttc
     7861 gacacgtttc tgagctgctt caacctgacg cgtcaacatg cgagactcaa tcgcctcttc
     7921 agacatgttc aagcgttcaa agattccctt caagcgttca gaaccaaaac gtttcatcaa
     7981 atcatcttca agagataggt agaattgtga ctcacctgga tctccttgac gacctgaacg
     8041 tccacgaagc tggttatcga tacgacgact ttcatgacgt tctgtaccaa taacacaaag
     8101 tcctccaagt tcacgaacac cttcaccaag cttgatgtcg gtaccacgac ccgccatgtt
     8161 ggttgcgatg gtaacggcac cacgttgacc agcattcatg atgatttggg cttctctata
     8221 gtggtttttg gcattcaaga cttcgtgagg aacaccagct gcaaccaatt tcttagaaat
     8281 gtagtcacta gtttcaaccg ctactgtacc aaccaagaca ggttgaccct tttggtaacg
     8341 agccttaacg tcttcgacaa ccgctttaaa cttagattct atacttgcat aaagaaggtc
     8401 tgagtggtca atacgttgaa caggacggtt tgttgggatt ggaataacac gaatgttgta
     8461 gatttcacgg aattcttctt cctcagtctt acctgtaccc gtcataccag acaatttctt
     8521 gtacatacgg aaaaggtttt ggtacgtgat tgaggcagat gtcttggttt catcctggat
     8581 tggcacacct tctttggctt caatagcttg gtgcaatcca tcagaataac gacgaccttc
     8641 catggtacga cctgtaaatt ggtcgacaat caagatttct tgctcttcgc tcaccacata
     8701 gtcaatatcg agaagcatga tgtagttggc acgaagggcg ttatcgataa agtgggtcaa
     8761 agccacgttt tcgatgtcat agaggttttc aagtttgaag tagctttcag ccctgtcaat
     8821 ccctgaatca gacaaaccaa tagtcttaga ctgcacatcg atgatgtagt catctttgtt
     8881 caaagatttt acatagtggt ctgccatgtg atacaactga ctggtttcaa ccgcattggc
     8941 acctgataca atcaaaggtg tacgagcctc gtcaatcaag atagagtcaa cctcatcgac
     9001 caaggcatag ttaagcggac gttgtaccat gttttcagcg cgaacgacca tgttgtcacg
     9061 aaggtagtca aatccgattt ctgagttagt tgagtaagta atatcacact cataggcttc
     9121 ttttttctcc attggagatt tggtagccaa gttaatccct actgacaaac caagccaaga
     9181 gtacaattca cccatctcag tcgcgtcacg ttctgacagg tattcattaa ccgtaactac
     9241 gtgaacccct ttacctgaaa gggcattgag gtataccggc atggtcgcag tcaaggtttt
     9301 cccttcccct gtacgcatct ctggcacgtc accatggtga agaacaatcc cccccatgac
     9361 ctgaacctta taagggaaga gacctaggac acgtttggca ccttcacgga caaccgcaaa
     9421 tgcttcgtaa agcaatgaat ccagtgattc tccattttga taacgttcct taaattcaac
     9481 tgtttttgct tttagttggt cgtcagtcaa agcagccatt tggtcttcgt atttgaaaac
     9541 cttgtcagcc atcttttcca gacgacggat ttctccttta tcattttcga taattgtttt
     9601 taaaatatta gccatgtttt tccttacttt aaattccgaa tattttagaa tgttccttta
     9661 attttagcac aattactgat tattttcaag gaagaatccc cattttgaaa aggaaaaaga
     9721 ggttagtttc caagctaacc tctctgactt ttatgatact ttaattgcca agaatcggca
     9781 aaatagcaaa taggataaat aagttaatcc aaagagaaag acagcagatc aatccaaaaa
     9841 caaagagact gactttcttg gacagcaagg ccatcaaaat ggacagaatg ccaaaaacta
     9901 gcaagacttt ctgtatgctg aagaggtcaa tcttcccccc cagaatcgga ctgccccaag
     9961 gaagaaagaa gaaagcaatc cagatcaaca gaactaaacc aatatagacc ttagaatagc
    10021 gtgtaataaa tgattttaga ttcaacatcc tatagctcca atcgtttcaa aataaactct
    10081 gatagcatcc gcaacccata aggcactttc tggtatccaa cgtctgttaa cttttataaa
    10141 atcgactaac tgacgattac actcacaatt agctccaata ccagctcccc acttgtaaca
    10201 tctatcatga ttttggcaac cttgatccaa aacgtctacc acaggtaaag taaatccttc
    10261 cccgttgtaa ccaggaccac agtaattccc gtgaacaggc aatcctgcct taaaatattt
    10321 agcaggtgta aacgtatttg aactatagtc ttcgcttatt gtctctacca ataaacccat
    10381 gtcaattaca tctttcgctt gtttatcttg aaccgcttta tcataatcaa aataaatcct
    10441 atctttatct tttacaagat aactcttcaa caattctccg ttacgaaaac ttctatcgct
    10501 aacttccaaa gagtttaagt tatcatgtga attagagtct gcaaaaacat tttttccagc
    10561 aaataaaagt aaaaaggcag ataaagcaac taaacaaatt ttaacgatag ttttcatgac
    10621 tactcctcct aataaacata agttataagc taaatacaca aacaatattt ttaccaaaag
    10681 aaagttaatt tttggtaaat gtactaaata cacataaact taaagcttgt caaatgagtt
    10741 taagaaatac atgttttata ttattctaac agatgatatt tcatatgttc tcctccttac
    10801 ctataagcta taaaatattt aagtttggct ataataaaac tacaaaaaat aagtatgtca
    10861 aacttaacta aagaatataa ataacacatc aattagtcac tcccaaaacc atatatcaaa
    10921 ccatggtatt aaaggctccc aatcattaca atatataaca aaattaagtg aatcttctat
    10981 ttccatgact tttccctcct ttttgatata ataataccac ctttttctac gttttttata
    11041 aatgtagaaa atattcacat ttacctagga ggaacaatat gagatgggat tatggacaga
    11101 tttttaaaga aattcgaaag tcaaaaggat tgacccaaca agatgtatgt ggacaagtca
    11161 tacatcggac aactctaaca aatattgaac acggtaaagt tattcccagt tttgaaaaca
    11221 tggtatttct tcttgaacaa attgatatga gcttggcaga attcaagtat atatggaacg
    11281 aataccatcc tagtaaaagg cgagatatta ttgtagagag ccaaaatccg tctacttttc
    11341 aagatactag aaaaatggtt gaactcactg agaaatgtca aaaatatctt aagacacatc
    11401 acgatgttcc tattcaaaat atctatcgtc atacaaaaat tgtcacagag ttacgaacta
    11461 aaggattcaa aaacaaccac gtcttgaaag atttgtctga agaaatttgg gactatcttg
    11521 aacctatgga tacatggtac attagtgatt tgaaattgct tggaaccatt ctctttttct
    11581 ttccttctga aaatcttccc cttcttattg atagaattat gaaaaccatc gagaaatata
    11641 aatacttccg agaaacaaaa gtatttttat catctttttt agccaatctc tccactgtat
    11701 attttcaaca tcatttattc aaagaatgtg aaacaatcac cctacaactt ttggtattag
    11761 cagaagaatt aaaaatatac gatattcttg gattctctca agtacgcttg ggaatactac
    11821 aacataactc cgatttgatt gataaaggaa taactttatt acgactaacc aaagaagagg
    11881 cattagttga aatactcgaa aaagaaatta atgatttttc aaacctataa tttgtaataa
    11941 ctctcatcat tttattatag tagattgtaa tagaatatga acgaatcaat taggggatct
    12001 aaagcatttt ataccaatgt tttataagtt gtattgtatt attccatatt caatccacta
    12061 tacaagatga atatttctct caagtttcat aatcaaaata aaaatgactg tatctatagc
    12121 tccataaaca aatcatagat acagtcattt tttagttcct acaccttcct ttatgcacac
    12181 ctttagactt ccctagttcc tgtctcaagc gaaacatttt tttgaaaaag acataggcta
    12241 accaatcact agcaaaagcg ataagtagta ataatagcca aacaccccaa gacttgatgt
    12301 ctgccacatt tctcaagacg atattgaaca acaggaccga aaaaactgtc caagccagac
    12361 taaaaagagc atagagggtg atgtaaatcc agtaaaaata gtaaaaaatt gggaaaatct
    12421 tactatctct gtttgccttt tcaatccagc tgaaaaagta aaaacagggt aataagatta
    12481 tcattttaaa caagtgttcc atcacaagcc tcctttttta aattgttttc tttagtttcg
    12541 ttctattaaa tgttattacc aatataaacc ttggaatagc gtgtaataaa tgattttaga
    12601 ggtaccataa gccacctcct acaaatagaa accgatataa atcaatgcct tccaccctta
    12661 gacttcccta gttcctgtct caagcgaaac atttctttga aacaggaata agttaaccaa
    12721 ttcataccaa tagctagcag aataaaaaga aaccaaatgc cccataactt gatatctgtc
    12781 acatttctca agacggtatt gaaaaacaga actgaaacaa ctgtccaagc aaggctaaaa
    12841 agagaataga aggggatgta aaaccagtaa aaataataaa aaattggaaa aaacttacta
    12901 tttctgttgg ccttctcaat ccagttatca aaataaaagt acggtgctaa aagtaagaat
    12961 ttaaacaaat gttccatcac cgacatcacc ctccttcttt tgatagcgtt ttctattatt
    13021 ttattatatc aaaaaaatcc ggaactgtca ttccagattc tactttttta tttgcgtttt
    13081 cttgcgatga gatgaatcgg tgttccctca aaaacaaagg ccttgcggat ttgattttcc
    13141 aagaaacgca ggtaagaaaa gtgcatgagt tcttcttcat tgacaaagat gacaaaggtt
    13201 ggtggtttgg ttgccacttg ggtcgcatag aaaatcttga gacgttttcc tttgtctgtc
    13261 ggtgttgggt tgatggcaat ggcatccatg atgacatcgt tcaagacagc tgatggaata
    13321 cgtgtgtttt gactttcgct gatttgctta atcatctcag gaagtttgtg gagacgttgc
    13381 ttggttaaag ctgatacaaa gataatcggt gcgtaaggca ggtattggaa ctgctcacgg
    13441 atatcttctt cccagttttt catagtgtgg ttatcttttt caagcgtatc ccacttgttg
    13501 accacgataa tcatcccttt accagcttca tgggcaaatc ctgcgatacg cttgtcgtac
    13561 tcacgaatgc cttcttccgc attgatgacc atcaagacca catctgaacg gtcaatagca
    13621 cgcatggcac gcataacaga gtatttctca gtattttcat aaaccttacc agacttacgc
    13681 ataccagccg tatcaatcat ggtaaactct tgaccatctg tatctgtaaa gtgggtatca
    13741 atggcatcac gagttgttcc agcaacagga ctagcaataa cacggtcttc tcccaagata
    13801 gcattgatca agcttgattt tccaacgtta ggacgaccaa tcaggctaaa cttaatgaca
    13861 tctggatttt cttcctcata ttcatttgga agattttcta cgatagcatc tagcacatcc
    13921 cctgtaccga ttccatggac agatgagata ggcagtggtt cacccaaacc gagagcatag
    13981 aaatcataga tatcatttct catctcaggg ttgtccacct tgttgactgc gaggataact
    14041 ggtttgtggg tcttataaag cttacgagct acgtattcgt ctgcatcagt aattccttcc
    14101 ttaccagaca cgacaaaaac gataacatct gcttcttcca tggcaatttc tgcctggtgc
    14161 ttgatttgtt ccatgaaagg agcatcgaca tcatcaattc ctcctgtatc aatcatacta
    14221 aaagagcgat tgagccactc acccgttgca tagatacggt cacgtgtcac tccttcgaca
    14281 tcttctacaa tggagattcg ctcaccagcg atccgattaa atagggttga tttcccaaca
    14341 ttgggacgtc ctacaatggc aatagttggt agggccataa tttctcactt tctacaataa
    14401 cttcttctgt tcaagatttt ttctagttga gcttggttca gcttgaccaa actgttctgc
    14461 taggcgctga ctccagcttg tggtcgcacg cgctccagca tagtcagcct gtacacggtc
    14521 ataagcttgg attgcctcag ttgactgttc ttggtattct tcctcaaaga ctacattatc
    14581 tagtggcagt cttggtttca catcatgagt ttgatttgga acacctagtg ccatcccaaa
    14641 gacaggatag gtgtagtcag gcaggttaaa gagttctgcc acttcttctg acttgtaccg
    14701 taccaagcca ataatcacac caccataacc caaactttca gctgccagca gggcattttg
    14761 tccagccaaa gccgcatcca ccgaactaat caagagagct tctacacctt ggggttggaa
    14821 ggtgtcggta tgaagtcggg ctcccttttc tgctcggttc aaatctccga caaagagaag
    14881 gaaaacagca gactggcgaa tggcttcttg aggtaccaat tcatacaagg catctttctt
    14941 ctcttgactt cgtaccacaa tcacagagta ggattggaaa ttcttccaag atgatgccat
    15001 ctgggctgct gtcaaaatct catttaagtc tacttgggga atttcttgct ctttaaacct
    15061 gcgcactgaa gtatgagcct tcatcaattt aatggtttct gtcatcgacg gtttactcct
    15121 tctaaacgag tctcctcagc caaataacgg atgcgttcca tgacccgtct ggcttcccag
    15181 gtttcgtcat ttccatgttt cactttcgca aaatgcttct ccaaatcttc aaagttgaag
    15241 ttggatgtga aaaaggtcgg taaattttcc tgcatccgat attggagaat gacctgcagg
    15301 atttcgtcac gcacccaaac ggttgattgc tcggcgccaa tatcatctaa aatcaggacc
    15361 tcagacagct taatctcatc caccaaggtc ttaacattgc catcactgat agcatttttg
    15421 acatcaatga caaagctagg atagtggagg agagttgatg aaacaccacg tttttctgat
    15481 aaatcatgag ctaaggccgc caccatgaaa cttttaccca caccaaagtc tccatataag
    15541 taaagacctt ttcgaatagc tggatattgc tccacgaagg ctagtagctt ttcaaaaact
    15601 ggtaagcgcc ccaaatcatc caagtcaact tgagccaaac tagctttctt gagactggct
    15661 ggtagattga ttaacttgag acggttctta atagccgctt ctttttcagc cgcgattagc
    15721 tcaggagttt cttcatatga aacatctgca tagccatgat tcttaaccaa aatcggcttg
    15781 tagcctttgg caatataatc cgtatcccca cggagaaact tgtcacgctc ggtgatgtac
    15841 tgattaaact tggagatact gcgatttaat tcctctggag ttaaggattc ttgctggata
    15901 aaggccgcaa catcagggtc cttcatgatt ttctggacca aatcttgata ataaaaacgg
    15961 ctgggttgac gtttgagtac gtctccgaca ctttccatct aatctcctcc tttttctaat
    16021 cgagctaata gttcttgctt cttacgttct agttccagac gagtttcctc gctggtttca
    16081 ttcttatatt caggattact ccatttagga acattggttt tttctggggc agtctgattc
    16141 tgtttttgtg tttttgcttt ctgccctcga tcacgaattc gtaaaacggc ctcttctgcc
    16201 gaatgaatct tttgataggc atagtcattg gctaccttca tggcatattt ctcattgata
    16261 tttgccgaat ccaccttatt aaaggtcaat aagagaataa tattgatgac ttcgtccagt
    16321 aagcccaagc cagccatctg ttgcaagagt tctctttctg tttgggtaat ggttcccttg
    16381 cgtgtttgct tgatttctgc taagaactgc agggcagttt tacttttagc ttctttgata
    16441 atggtcgctt ccttaagact aaagtcagag gaaactggtt tttgagcaat tttttcacgc
    16501 atgcgtttgg ttgaaataac ctgggaaaca gctgttgact tggccaattg ataggtttca
    16561 aaccaagtcc atttcttctc ctcggcaata gcaaagaggt ttaagacatc ggactgctca
    16621 tccgcaaaac gaagtccatc tcgagccatc agctggcgaa aatgttccaa gtcaaaatca
    16681 ttggccactt tcttcttgag accaaggtct tcttgactgc ctagttctgc caattctgga
    16741 aagacttgat tgagtgagac aggtatttct tcaccatcag cactttcaac tttcaaatcc
    16801 tccacagcta catcgccaat ctttttctct aagagtctgc gataaacagg atgccccaag
    16861 aagtcttgac tagatagagg agcatggagg gctagctgat aaacatcacc cttttgatag
    16921 agggtcaaga gattaaaagc agataagatt ttcaatgatt ttatcagtct atccatccca
    16981 aagttgagat ggttgagaat gcttgaaaaa agatattcct ttctaccatt atcccaaaaa
    17041 ctgattgtat aaagataaag gctcagtgcc tcctgaccga taatcgggag gtagcactgt
    17101 accagagatg aggtatcttg cgacacccga ttattcttta gataagaaaa acggtcaatt
    17161 ggcttcattt atctttcctt tttcttttta gaggactggg tgatttgttg gagcaagctc
    17221 tctaactcac tgacatcctt aaaactacga tagacactag caaaacgtac ataggtaatc
    17281 tcgtccaatt cagccaactc ctccatgacg agtgaaccaa tgtcctcact ttgaatttca
    17341 ttttcatttc gaccacggag tttctgttcg atacgattga ctaccatgtt gatttcatca
    17401 cttgacacag gacgtttctg ggctgagcgg ataatcccat taaagatttt atctctggag
    17461 aattgttccc gtgtgccatc ttttttaaca accactaagg ttctttcttc tactcgttcg
    17521 taggttgtaa aacggtgttg gcattcgtcg cactcacgtc ttctacgaat ggtgttccct
    17581 tcttctgctt ggcgactatc gataacactt gacttggtag ccccacattt tggacaacgc
    17641 atcctttccc tccttatcgt tttcttttca ttataccatt ttttaagcga ttcccaaaac
    17701 aattcttctt tttgcttgac aagttttttg ttttgttgta ttatttaatt aagacaacaa
    17761 ggtaaaagaa aggagactaa gatgtcctgg acatttgaca acaaaaaacc catctattta
    17821 cagattatgg agaaaatcaa gcttcagatt gtttcccata cactggaacc caatcaacaa
    17881 cttccaaccg tgagggagct agctagcgag gctggtgtca atcccaatac catccaaaga
    17941 gccttatcag accttgaacg agaaggattt gtctacagca agcgaacaac tggacgattt
    18001 gtgactaagg ataaggagct aatcgcccag tcacgcaaac aattatcaga agaagaattg
    18061 gaacacttcg tttcctccat gacccatttt ggctatgaaa aagaagaact accaggcgta
    18121 gtcagtgatt atattaaagg agtttaagcc tatgtcatta ctagtatttg aaaatgtatc
    18181 caaatcatat ggagcaacac cagcccttga aaatgtttct cttgacattc cagctggaaa
    18241 aattgtcggc cttcttgggc caaacggctc aggaaaaaca accctgatta aactaattaa
    18301 tggcctctta caaccagatc aaggacgtgt cctcatcaac gacatggacc caagcccagc
    18361 aaccaaggcc gttgtagctt atttgcctga tacgacctat ctcaatgagc aaatgaaggt
    18421 caaagaagcc ctaacctact tcaagacctt ctataaagat ttcaatcttg aacgcgccca
    18481 tcatctactt gcagacctgg gcattgatga aaatagtcgt ctcaagaaac tatcaaaagg
    18541 aaacaaagaa aaggttcaac tgattttggt tatgagccgt gatgctcgtc tctatgtttt
    18601 ggatgaaccc attggtgggg tggatccagc agcacgtgct tatatcctca ataccattat
    18661 caacaactac tcaccaactt ctaccgtttt gatttctacc cacttgattt ctgatatcga
    18721 gccaatcttg gatgaaattg tcttcctaaa agacggaaaa gtcgtccgtc aaggaaatgt
    18781 agatgatatt cgctacgagt caggtgaatc cattgaccaa ctcttccgtc aggaatttaa
    18841 ggcctaagca aaggagatta tttatgtttt ggaatttagt tcgctacgaa tttaaaaatg
    18901 ttaacaagtg gtatttagcc ctctacgcag ccgtgctagt cctttctgcc ctcgtcggaa
    18961 tacagacaca aggctttaaa aatctacctt accaagaaag tcaggctact atgctacttt
    19021 ttctagctac agtctttggt ggcttgatgc ttacacttgc gatttcaacc attttcttga
    19081 ttattaaacg cttcaaaggt agtgtctacg accgacaagg ctatctgact ttgaccttgc
    19141 cagtttctga gcaccatatc atcacagcca aactaatcgg tgcctttatc tggtcattga
    19201 ttagcacctc tgtcttactc ctatgtatct atattatctt attgtcaaca aattctaatg
    19261 aagttcaact ttctactctt ctgtcatttg ttggagaaca ttttaccggc tttagtctta
    19321 ctgtcttatc cttcctacta aatactattt caggaatcct ctgcatctac ttggccattt
    19381 ccattggaca actgttcaat gaataccgta cagcactcgc tgttgcagcc tacattggta
    19441 tccaaatcgt cgttggcttt attgaagtct tcttcaatac cagtactaac ttctatatta
    19501 attcacttgc aggatttaat gataatttct atatgggagc aagcataggc attgttgaag
    19561 aactcatatt catagctatc ttttatctcg gaacctacta catcttgaga aataaggtta
    19621 atttgcttta aataattttt acctagatat gtaacatact catagaacaa aagagaccag
    19681 gcaaaaagtc tttaaaatta gaaaacgcat agtatcaggt gttgaatatg tactgccccc
    19741 caaaagttag attttttctg tctaactttt ggggggcagt tcataagaac cttggtaata
    19801 tgcgtttttt gtgagctgac ttatttcctt tcactatatc gcaaaatgaa ataagaacgg
    19861 aacgatggga ttttggaatt caaatcaatt tataagaatg ttttagaagt aatattatcc
    19921 tattccagat tcagttcact atacaattga gttttcaagc aacctgttta cataatgtgt
    19981 acataattag gttcgtgatt ccaccctttt cacctttaaa aacctcgctt tcgcaaggct
    20041 cttctattta taagataagg cacgtttaaa ggttttccaa atccctaaat catccgtttg
    20101 aagaacgaga ctagcataca tgcgtccgat aaatcctgtt gctaccaccg caaaaatcac
    20161 tgtaatagca agtgaaatcc atgcttccgc tccctccgca tagccattaa tcgttcgaaa
    20221 cggcataaag aaggtcgaaa taaagggaat ataagaacca atcttcaaga ggagattgtc
    20281 accagctgca cctagagctg tcactccaaa aaaactaccc ataatcaaaa tcatcaaagg
    20341 cgacaaggct ttccctgagt cctcaggacg agaaaccata gatcctagga aggctgccaa
    20401 gactacgtac atgaaaagac tgatcaaaat aaagagcagg gtattcagtg agatagcatc
    20461 tcccaagtga accaaaatac cagactgagc caagaatggc aaatctttaa agagcaaaac
    20521 ggcagccaga ccacctacaa catagatccc aatatgcgtt aaaatcacta gaaacagagc
    20581 catcatccgc gcatagaaat agtgacttgc ccttatgcta gaaaaaacga cttccataat
    20641 tttggtgcct ttttcactgg caacttcctg agctgttaca cccgcatagg taatcagaat
    20701 catataaaga aagaatccta aggcacctgc tgcaattgtt tgaataaact ttttattttc
    20761 cttggcttca tcaatctttt ctgtgaattg aattgtctgc gctaagcgtt tttcctgctc
    20821 ttgagacaag gaagcagttg aacgattaag ctgattttgc agttcattga gtgtacctgt
    20881 aacctcaaat ttaattccat tttcaagcga tgtttcgcca tgataaactg cctttagaac
    20941 actatcttct tgatcaatgg tcaaataacc ttttaatttt tcttctttaa ttgcttcttt
    21001 ggcacttgct tcgtctttat agtcgaagtt aacaccattt acattcttca gtccttctgc
    21061 tacagatggc actgttgtca ctactgccac tttattattt ttagccatag aagaaccttg
    21121 gatatgccca attcctacag agattcctaa aaagaggaac ggcgaaatca ccataaagaa
    21181 gaaactccat gacttgacat gtcgaagata ggtttccttg attacaaccc acatatttct
    21241 catacttcca ctcctgattc tagtttaaag atttcatcga tagttggcgc ttgttggtca
    21301 aatgttgcga tatattgacc ttgagtcaag attgggaaga gttcccttcc agcgctctca
    21361 tcctccaaaa tcaatttcca actgccttgt ttggtcaagc tcacctgttt gacatgagga
    21421 agattttcca attcttcctt gcttcgttca cttgaaacaa agagacgcgt tttcccgtat
    21481 tgattgcgga catcctgaac tggtccgtgc aagaccacac ggccatctcg gatcatcaga
    21541 atatcgtcac aaagttcctc aacattggtc atgacatggt cagaaaagat aatggttgct
    21601 ccgcgctctt tttcctgaaa aatgacttgt ttgagcaatt ctgtattaac tgggtccaat
    21661 ccactaaaag gctcatccaa gataatcagg tctggttcat gaatcagagt aataatgagc
    21721 tgaatcttct gctgatttcc ttttgacaga ctcttgattt tatctgtcag ctttcctttc
    21781 acttccaacc tcttcatcca ttgagggagt ttttctttga cttctttggc atccatgcct
    21841 tttagagtcg ccaagtagcg aacttgttca agaactgtca atttaggcat gagactgcgt
    21901 tcttcaggca gataaccaat ccgagcatag gtctcctgac gaatatcctg accatccaga
    21961 ccgatttctc cctgatattc taggaatttt aaaatactat ggaaaatcgt tgtttttcca
    22021 gcaccatttt ttccgactag tcccaaaata cgacctggtc gcgcttgaaa gtcaatacca
    22081 aacaaaactt gcttggatcc aaaacttttc tctagacttc ttacttctag catctttcac
    22141 ctccgaaatt tcttgcactc attatactcc tttttgatag cctttacaat gtttttgtcc
    22201 atttttagaa gactattgct gtgtaaaata tggcctggag cacttttata ctcaatgaaa
    22261 agcaaagagc aaactaggaa gctagccgta ggctgctcaa agtacagctt tgaggttgca
    22321 gataaaactg acgaagtcag ctcaaaacac tgttttgagg ttgtggatag aactgacgaa
    22381 gtcagtaact atatctacgg caaggcgaag ctgacgtggt ttgaagagat tttcgaagag
    22441 tattagtgat aaatccatta tacagtagca aacttaattt ataccttccg ctcctcaact
    22501 gtctattttt aatcctgaat tgttatttga gtaactcctt tttcctcata aagttttctt
    22561 cctctaaaac ttctggaaaa aagcgaatag tttcagacaa catttttata agaaacaagt
    22621 tcatctgtca tttcaagaag gagtaatcct ttatctacta atggacggaa cggaattcaa
    22681 ccgcttgtcc gatatgtttt ctaaggatta tatagcaaaa tgaaataaaa acaggacaaa
    22741 tagatcggga cagtcaaatc gatttctaac aatgttttag aagtagatgc gtactattct
    22801 agtttcaatc tattatatct attatgcaca ccactatagg atctaataaa aatcacaaca
    22861 agttcattca tagatggtta cctcagggaa ctaagaaaac gactacaaag gaagtcgcat
    22921 tcatcgaaaa gtggattaac aactatccta aaaaatgctt gaactacaag tcccccagag
    22981 aagacttctg gatgactaac ttgaacttga aatttagcaa taattaattc accatctaac
    23041 tatatttagt aattatttca gaactgatta atattaaaat taactaacaa ttcaaaggat
    23101 tcatactagc cataaattac gtccatcaga gagagactct tactactttt agattttagt
    23161 ctttctagct tcagaataca tctaaacttt aggaaaaatg actattcgaa agcgcgaatg
    23221 cctcaaaatt atctcagata agctattcga aacttagaat gcttttaaat ttatggaatt
    23281 gcgattattc gaaacctaga atgcatataa cctttagttg acagacctat tctaagtctc
    23341 gaagggctat ttactttcta ttccttatca aaaaagactc attccccctt tctcctccaa
    23401 aatatggtat agtagaaata tactatctat gaggagttta catgtcacag gataaacaaa
    23461 tgaaagctgt ttctcccctt ctgcagcgag ttatcaatat ctcatcgatt gtcggtgggg
    23521 ttgggagttt gattttctgt atttgggctt atcaggctgg gattttacaa tccaaggaaa
    23581 ccctctctgc ctttatccag caggcaggca tctggggtcc acctctcttt atctttttac
    23641 agattttaca gactgtcgtc cctatcattc caggggcctt gacctcggtg gctggggtct
    23701 ttatctacgg gcacatcatc gggactatct acaactatat cggcatcgtg attggctgtg
    23761 ccattatctt ttatctagtg cgcctatacg gagctgcctt tgtccagtct gtcgtcagca
    23821 agcgcaccta cgacaagtac atcggctggc tagataaggg caatcgtttt gaccgcttct
    23881 ttatttttat gatgatttgg cccattagcc cagctgactt tctctgtatg ctggctgccc
    23941 tgaccaagat gagtttcaag cgctacatga ccatcatcat tctgaccaaa ccctttaccc
    24001 tcgtggttta tacctacggt ctgacctata ttattgactt tttctggcaa atgctttgac
    24061 acgtaaaaaa tccgtttggt ttcccaagtg gatttttaaa gcgtagatta actatagctt
    24121 gatactaaat atactttggt atggaaatca tgcatatttt tcgatagtga ggcgaggact
    24181 tacctagcct ttccgccgtg atagaaacac ctgaaatcta atggtttcag gtattcggaa
    24241 actttgagcc tagtgtctca aagtttaggt atggaatttc gaagaaagtc gctaccgtcc
    24301 gtaatcactt aaggaaaggc tcaaaaatat tgttttcaac cacaaaatcc gtttggtttc
    24361 ccaagcggat tttgtgcttt attttgaaac ttcttttgca agaacaaagt tcccaagtgt
    24421 ggcagaacca tttcctgcga ctgctggcgt cacgatatag tcacgcacat ctggtactgg
    24481 taggtaacca ttaagaagag atgtaaattt ctcacggaca cggtccagca tatgttgttg
    24541 agccatgacc cctccaccaa agacaatcac gtctgggcgg aaagtcactg tcgcattaac
    24601 cgcagcttga gcgatatagt aggcttgaac atcccaaaca gggttgttga gttcaatagt
    24661 ttccccacgt acacctgtac gagcttccaa acttggacca gctgcataac cttctagaca
    24721 tcccttatgg aaaggacaaa cacccttaaa ctctttttca atatccattg ggtgtctagc
    24781 aacataataa tgacccattt cagggtgacc cacaccaccg ataaactcac cacgttggat
    24841 gacgcctgca ccgatacctg taccgattgt gtagtaaacc aagttttcga tacgaccacc
    24901 agcattgtta cgggcaacca tttcaccgta agcagagctg tttacgtctg ttgtgaagta
    24961 cattggcacg tttagggcgc gacgaagggc accaagcaag tctacatttg cccagtttgg
    25021 ttttggagtc gtcgtgataa agccataagt ttttgagttt ttgtcaatat caatcggccc
    25081 aaatgaacca actgcaagac cagcaaggtt atcgaatttt gagaagaact caatggtttt
    25141 atcgattgtt tcgattggag ttgttgttgg aaattgtgtt ttttctacaa cgttaaagtt
    25201 ttcatcaccg acagcacaga caaactttgt accgcccgct tccaagcttc catataattt
    25261 tgtcatgata aacctcttgt ttttattttc tttattatag catacttcga aagtctaaat
    25321 gtctctattt tttagatttt cctctgtaaa tcttactatc taataaaaac gaacaaacat
    25381 gtcatttgtt cgttttcaca ttagagagga ttgattagat tttcacttcg atcacagcat
    25441 cccccttcga aactgaacct gttgcgactg gagctactga agcgtagtca gctgtatttg
    25501 taacgataac cattgttgta tcatcaagtc cagctgcagc gattttgttt gagtcaaatg
    25561 ttccaagaac atcgccagct ttcaccttat taccttgagc aacttttgct tcaaaaccgt
    25621 caccgttcat agatacagtg tcaataccaa cgtgaatcaa aacttcagca ccatttcttg
    25681 ttttcaaacc aaaagcgtgc cctgttggaa aggcaattga aacttcagca tcagctagtg
    25741 catagaccac gccttgactt ggtttcacag cgataccttg tcccatagct ccacttgaga
    25801 agactgggtc attgacatca gcaagagcga caacatcacc gacgatagga gttacaagtg
    25861 tttcattttg aagagctgct ggcgcaactt cttctttttc ttcagccact tcagcttgtt
    25921 ttgcagctgc agttgcgtct acttcatctt cgtaaccaaa catgtaagta agagcaaaac
    25981 caagggcaaa tgatacagct accataagaa ggtattgtgg aagttgtccg ttaccaacat
    26041 aaagcattgt accagggatg atggtgatac cattaccagt accagcaagt ccaaggatag
    26101 aagccaatcc accaccgatt gcaccagcaa tcaatgaaag gaagaatggt ttacggaagc
    26161 gcaagttcac cccgaagata gcaggctctg taatacctag gaaggcagaa agagcagccg
    26221 ggaaagcaag tgttttcagt tttggatttt ttgttttaac accaaccgca acagtagcag
    26281 caccttgagc tgtcatagca gctgtgatga tagcgttgaa tgggttagca tggtcagcag
    26341 caagtaattg cacttcaagc aagttgaaga tgtggtgcac acctgacacg acgatcaatt
    26401 ggtgaacccc accaatcaag aaaccaccaa gaccaaatgg catgctaaga atcgcttttg
    26461 tagcaataag gatgtagttt tcaacaacgt ggaaaactgg tccaatgaca aagagtccaa
    26521 ggatagacat gaccaaaagt gtcacgaatg gtgttaccaa gaggtcaatg acatctggaa
    26581 caaccttgcg gacagctttt tcaaatttag ctccgacaac cccgatgatg aaggctggaa
    26641 gaacggaacc ttgcaaacca acaacaggga tgaaaccaaa gaagttcatc gctgttactt
    26701 caccaccttg agcaactgcc caagcgtttg gaagtgagcc agagacaagc atcataccaa
    26761 gaacgatacc aacggcagga tttccaccaa atacacggaa ggttgaccac acaaccaaac
    26821 ctggcaagat gatgaaggct gtatctgtca agatttgtgt gtaagttgca aagtcacctg
    26881 gaagtggcat ttcaagagcg ttgaaaagac cacgcacacc catgaagaga cctgtcgcta
    26941 cgataactgg gatgattgga acgaaaacat caccgaaagt acggatagca cgttggaacc
    27001 agttcccttg tttagcagct tctgctttca tgtcatcctt agatgatgtt ggtaagccaa
    27061 gtacaacaac ttcatcgtac attttgttaa ctgtacctgt accaaagata atttggtatt
    27121 gccctgagtt aaagaaagca ccttgaactt tttccaagtt ctcaatcact tctttattga
    27181 ttttctcttc atctttgacc atgacacgta gacgagtcgc acagtgggca acactattga
    27241 cattttcacg tccgcccaag gcatcgatga ctttttttgc aatttcctga ttgttcattt
    27301 gcaaaaatct ccttatataa cattttgttc ttgtttgaaa gcgattttat tcgcccttac
    27361 gactattatt ttatcatgtt ttaaaaatat gtcaagcgtt ttgcataaaa aatattctat
    27421 ccacttaatg ggcaagcttt agattgattg tttttgactg tttcatcagg aattccttga
    27481 aaaataagtt tgaaaccttg gttaaaaagt cttttatttc attatctttc acattttcgt
    27541 aaaaatttaa ttgatttcct gtatttattt tatgataatc gtttgatagt ttttcctctt
    27601 ttttaacata aaaggcttgc attttttatc gaaaacagtt actattaaaa gtgataagat
    27661 ttttggagga atgaatggaa tggacaactg agcgtcgtta cagactttat caagattgga
    27721 cgcaagaaga aattcaacat ataaaggaaa atatggcaca atctccatgg catactcatt
    27781 accatgttga gccaaaaaca ggacttctca acgacccaaa tggcttttct tactttgatg
    27841 gcaagtggat cctcttttac cagaattttc cttttggtgc agcccacggt ttgaaatctt
    27901 gggcacagct agaaagtgat gatttggttc actttaaaga aactggaatc aaagttttac
    27961 cagatactcc attagatagc cacggtgcct actctggttc tgccatgcaa tttggcgata
    28021 acttattcct attttataca ggaaatgttc gcgatgaaaa ctggatccgt cacccatacc
    28081 aaatcggtgc tttgatggac aaggagggta agattacaaa gattgacaag atcttgattg
    28141 accagccagc agactctact gaccacttcc gcgatccaca aatttttaac tttaaaggtc
    28201 aatactatgc tgtcgtcggt ggacaggact tggagaaaaa aggcttcgtc cgtctctaca
    28261 aggctgtcaa taacgactac acaaactggc aagcagttgg cgaccttgac tttgctaacg
    28321 atcgtactgc ctacatgatg gaatgtccta atctggtctt tgtagaggaa cagcctgtcc
    28381 ttctctactg tccacaagga ttggataaga aagttctaga ctacgataat atctttccaa
    28441 atatgtataa gattggggct tcctttgacc ctaaaaatgc caaaatggta gatgtgtctc
    28501 aacttcaaaa catggattac ggtttcgaag cctatgcaac tcaagccttc aacgctcctg
    28561 atgggcgtgc tctagcagtt agctggcttg gtttgccaga tgtttcttac ccatctgacc
    28621 gttttgacca ccaaggaacc ttctctttgg tcaaggaact cactatcaaa gacgacaagc
    28681 tctaccagta tccagtcgct gctattaagg accttcgtgc ttctgaagaa gccttctcaa
    28741 accgttccca aaccaagaac acttacgaac ttgaactcaa cttggaagct aatagccaga
    28801 gcgagattgt cttacttgct gataaagaag gtaagggact ttcaatcaac tttgaccttg
    28861 taaacggtca agtgacagtg gatcgtagcc aggctggaga acagtatgcc caagaatttg
    28921 ggacaactcg ttcttgccct atcgagaatc aggctactac tgctacaatc ttcatcgata
    28981 actctgtctt tgaaattttc atcaataaag gagaaaaagt attttctggt cgtgtcttcc
    29041 cacatgcgga ccaaaatggt atcctgatca aatctggaaa cccaactgga acttactatg
    29101 aattagatta tggtcgcaaa actaactgat gtcgccaaac ttgcaggcgt cagtcctact
    29161 accgtttctc gggttatcaa taaaaaaggg tatctatctg agaaaaccat ccaaaaagtc
    29221 aatgaagcca tgcgagaatt gggctataaa cccaacaacc tggctcgtag tctgcaagga
    29281 aaatcagcta agttaatcgg cttgattttc cccaatattt ccaatgtttt ctatgcagaa
    29341 ttgattgata aattggaaca ccaactcttc aaaaatggtt acaagaccat catctgcaac
    29401 agtgaacatg attctgagaa ggaacgcgaa tacatcgaaa tgttggaagc caatcaggta
    29461 gacggcatca tttctggtag tcacaaccta ggaatcgaag actacaatcg tgtgacagcg
    29521 ccgattattt cctttgaccg aaacctatcg ccagacatcc ctgttgtctc ctctgacaac
    29581 tatgctggtg gggttcttgc tgcccaaacc ttggtcaaga caggtgccca gtctatcatc
    29641 atgattacag ggaatgacaa ttccaattcg ccaaccggac tgcgccacgc tggttttgca
    29701 tccgtactcc caaaagctcc tattatcaat gtttccagtg acttttctcc cgtcagaaaa
    29761 gaaatggaaa tcaagaatat cttgacccgg gaaaaaccag atgccatttt tgcttcggat
    29821 gatttgacag ctattctggt cattaaaatc gctcaagaat tgggcatttc tgtcccaaaa
    29881 gagctcaagg tcatcggcta tgatgggacc tactttatcg aaaattacta ccctcaattg
    29941 gctactatca agcaaccttt ggaagagatt gcttgtctca ctattgatct tctcttgcaa
    30001 aagattgaag gcaaggaagt cgccacaact ggttacttct taccagttac actattacca
    30061 ggaaaaagta tttaaacaca agaaaactca gaccgattcg tctgagtttt tatgatctta
    30121 aattttcgag atagcgctgg gctgtctcta ggttaaaggt tttatctgag atgaggcgct
    30181 ctactagggg agcaacttca gattcactag ccccagctag gagagctagg gatttggcct
    30241 gtagtttcat gtggccttgc tggatgcccg tacttaccaa ggctttgagg gctgcaaaat
    30301 tttgagcaag accgatggac acgataatct gggctaattc tctggcagaa ggatttccta
    30361 gtagatcatg actgagagct acacgtgggt tgaggccgat agagccaccc ttagtcgcta
    30421 caggcatggg cagggtcatc tcaccgacca attcttctct ttcaaggtcc agcgtccagc
    30481 agctaagacc ttgatagcgt ccatctcgac tggcaaaggc atgggcccca gcttcgatgg
    30541 cacgccagtc attaccagtg gcaatcaaaa tcgcatcaat accattaaaa attcctttat
    30601 tatgagtagc agctcggtaa ggatcagcct gcgcaaactg actagccaac gcaattttct
    30661 ccgcaatctc tcgtccttga tccttttggc ggctcaagta gcgaaaggcg atgcgacagc
    30721 ttgcagtcac cagagaatca gtcgcgtagt tggacaggat tcccatgaga ctctgtccct
    30781 gactgagttc ttctaagact ggtttcaagg cttccagcat ggtgttgagc atattggcac
    30841 ccatggcttc ctgggtatcg acatgaatat aaacaacgag aaagtctggt tcgcctttta
    30901 tctgctcgac atgcagatca cgcgccccac ccccacgttt aacgatagaa ggataggctt
    30961 gattggcaag ctccaagagc tccgctttct tgctggcaat cttctcttgc gctagtttag
    31021 gattagcaac ttgataaagg gctacctgcc caatcatctg gcgctgatgg acttgtgcag
    31081 taaaaccacc tgcacgcttg atgattttgc tggcatagct ggccgccgca accacagagg
    31141 gttcttctgt cacataggga acggtgtatt cctgaccgtt gacaagtacc tccggaacca
    31201 gcgaataagg cagagaaaaa gttcccacca cattctcact cagctggtct gccacagtca
    31261 cactcatctg ttcatccttc tccagactag cttgtctctc aggactaagg agcgcctgag
    31321 cttttagcag ctcgaggcgc tcttggtatg attttttaga aaatccattc caacttatct
    31381 tcattatttt tcaaccttgc tataacggcg ttggtggtca agaatttcaa ccaaggcaaa
    31441 atcttgattt tcatagccag caaactgggc agagtttgtt tcatccaagt ttacttcctc
    31501 aaaaaagacc ttttcatagt ctgcaacgga tagggcagtt cgttggttga gcttgttcaa
    31561 gcggtcttta tccaaataag cttcatatcc ttcaaccaat tcaccactga agaactcagc
    31621 cacagctcca cttccgtaac tataaagggc gattttatcc ccagctttca agctatctgt
    31681 attttccaag agagacaaaa gtccaaggaa aagtgaacct gtgtagatat tccccacctt
    31741 ttgactgtag agaatagact ggtcaaaatg cttttgtaag aggtcttttt tctcttgagg
    31801 caggctctta tccatgattt ttttcaagcc ttttagcgct aatttaggat aaggcaagtg
    31861 gaaacaaaca gccgcaaaat catccagagt aagctggtag cgtttttgat attcaagcca
    31921 agtcgttttc aaactatcca agtattgttg ggtagaatag acaccattta cataaggagt
    31981 tgtcgagtaa tttggtcgcc agaaatccat gatgtcacgg gtctgagcta cattgtcatt
    32041 attaaaggcc atcatgcgtg gattttgtgt aatcaacata gccacacttc cggcaccctg
    32101 agtgggttct cccggagttt caataccata tttggcaata tcactggcaa tgaccaagac
    32161 cttggactcc ggagaatttt ccacatgcaa tttggcataa tggagggcag ctgtcgcccc
    32221 atagcaggct tctttaatct cgaaactacg agcaaagggc tggatgccca gcaagccatg
    32281 cacaaagacg gccgcagcct tactctggtc aattcctgac tcggtcgcca caatgaccat
    32341 gtcaacttct tgtctttctt gctcagttaa aatagagtca ctagcactgg ccgccaaggt
    32401 cacgatatcc tcagttaggg gcgcaatact caattccttg agtaagagtc ctttacttaa
    32461 tttttcaggg tcaattcccc tcgcttctgc taagtcttgt aatttcaaga catattgact
    32521 ggtcgcaaaa ccaatcttat caataccgat tgtcatattt acctctgttt tatcattcat
    32581 gtaaaaaatc gttctatact attttatcac aaatggcagt aaaagagaga aaaaagactt
    32641 gattcaccaa atcaagcctc ttattggtca tcattttaaa gaatgattag ttgctagaga
    32701 gttcaccgat ataagtagct ttataagctc cattcacagt tatcagctcc tggaggatca
    32761 aatttcctga gtaagtcctt cccatctcat ctacaaattt ttgataaaac tgactggtcg
    32821 gaatttctct gacatcctta tcaaatgtct tgtcaagtgt tttactaacc ttctcagcaa
    32881 tcaattgatg ctcttaccat ccactttgaa actctgagcc cgaactagaa accatgactg
    32941 ggataaacaa caaggtcagt agatttacag acaataagga aagtagtaga cttcctgcaa
    33001 aactagaatc ctagttcatg attgataata ccagcactca aattcattcg taatccgaag
    33061 cgtttacgat gatttcgata ggttgt
//
LOCUS       DBERMR010000023        32023 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.023, whole genome shotgun sequence.
ACCESSION   DBERMR010000023 DBERMR010000000
VERSION     DBERMR010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 32023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32023
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.023"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            7..1686
                     /locus_tag="ACL8A1_001134"
                     /pseudo
     CDS             7..1686
                     /locus_tag="ACL8A1_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1954..2520
                     /locus_tag="ACL8A1_001135"
     CDS             1954..2520
                     /locus_tag="ACL8A1_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201918.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGVEV
                     EYFNPKNIPDYMEIIFNGNIVPLSRFMFDPGENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            2842..3030
                     /locus_tag="ACL8A1_001136"
     CDS             2842..3030
                     /locus_tag="ACL8A1_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HGS1201919.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            3092..3850
                     /locus_tag="ACL8A1_001137"
     CDS             3092..3850
                     /locus_tag="ACL8A1_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HGS1201920.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEAVLYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVHDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLF
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVIF"
     gene            3908..4762
                     /locus_tag="ACL8A1_001138"
     CDS             3908..4762
                     /locus_tag="ACL8A1_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HGS1201921.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGIWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCSIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            4874..5755
                     /locus_tag="ACL8A1_001139"
     CDS             4874..5755
                     /locus_tag="ACL8A1_001139"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HGS1201922.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKE"
     gene            5899..6525
                     /locus_tag="ACL8A1_001140"
     CDS             5899..6525
                     /locus_tag="ACL8A1_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201923.1"
                     /translation="MKDKESYNKFIPKLYNEELLKNHFKETMCFSRKELNNKSIINQK
                     LIKFPIVGHEVWALFGKEKTGEWRCLQVGQSKNKVKAEIESLIEFMSYDYNQLVDSIK
                     DGVKNRNSTFYSNIYQSSKKSKNEFLYSHIASQYDEFQLCLLDIDKYLGFKNLKFENK
                     HISNIMKIAKPLYAEAKLAFETKSIYWTMYNSGVDGQAIMIFLGDNKD"
     gene            6821..8560
                     /locus_tag="ACL8A1_001141"
                     /pseudo
     CDS             6821..8560
                     /locus_tag="ACL8A1_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
     gene            8835..9290
                     /locus_tag="ACL8A1_001142"
     CDS             8835..9290
                     /locus_tag="ACL8A1_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201924.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSGNLQP
                     EKWNETAKQLYNIIMWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKRVNTTIKN
                     GSKGKTLTEMINGYRADNDIRDEICNFNILKNKIRDMKDQQGNTMESYF"
     gene            9831..10304
                     /gene="tnpA"
                     /locus_tag="ACL8A1_001143"
     CDS             9831..10304
                     /gene="tnpA"
                     /locus_tag="ACL8A1_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISSpn6 family
                     transposase"
                     /protein_id="HGS1201925.1"
                     /translation="MREDNQSLSHTTWNCKYHIVFAPKYRRQIIYGRYKASIGRIIRD
                     LCERKGVIIHEANACSDHIHMLISIPPKLSVSSFMGYLKGKSSLMIFDKHANLKYKYG
                     NRKFWCRGYYVDTVGRNQKVIAEYIQNQLQEDRVADQLTLFESVDPFTGEINKRK"
     gene            10560..10781
                     /locus_tag="ACL8A1_001144"
     CDS             10560..10781
                     /locus_tag="ACL8A1_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS1201926.1"
                     /translation="MYRRLRDLREDHDLTQKQIAKILSFTNSAYAKIERGEHTLTADV
                     LVTLSNFYDVSTDYLLELTDFPDKIRFRK"
     gene            11227..11529
                     /locus_tag="ACL8A1_001145"
                     /pseudo
     CDS             11227..11529
                     /locus_tag="ACL8A1_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            11685..12914
                     /locus_tag="ACL8A1_001146"
     CDS             11685..12914
                     /locus_tag="ACL8A1_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HGS1201927.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNRLLK"
     gene            12934..15630
                     /gene="ppc"
                     /locus_tag="ACL8A1_001147"
     CDS             12934..15630
                     /gene="ppc"
                     /locus_tag="ACL8A1_001147"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HGS1201928.1"
                     /translation="MSLQKLENSSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDVYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYEIILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    15713..15930
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            16043..17047
                     /gene="trpX"
                     /locus_tag="ACL8A1_001148"
     CDS             16043..17047
                     /gene="trpX"
                     /locus_tag="ACL8A1_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS1201929.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     IELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            17116..17982
                     /locus_tag="ACL8A1_001149"
     CDS             17116..17982
                     /locus_tag="ACL8A1_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1201930.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            17979..18737
                     /locus_tag="ACL8A1_001150"
     CDS             17979..18737
                     /locus_tag="ACL8A1_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1201931.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEELTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            18827..20587
                     /gene="dnaG"
                     /locus_tag="ACL8A1_001151"
     CDS             18827..20587
                     /gene="dnaG"
                     /locus_tag="ACL8A1_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HGS1201932.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVTFMEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKTSEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAAILKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYAQIEQIVNESRQVQRQNRMEGISRPTPITMPIT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            20590..21699
                     /gene="rpoD"
                     /locus_tag="ACL8A1_001152"
     CDS             20590..21699
                     /gene="rpoD"
                     /locus_tag="ACL8A1_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HGS1201933.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            21713..22042
                     /locus_tag="ACL8A1_001153"
     CDS             21713..22042
                     /locus_tag="ACL8A1_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HGS1201934.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            22157..23200
                     /gene="cpoA"
                     /locus_tag="ACL8A1_001154"
     CDS             22157..23200
                     /gene="cpoA"
                     /locus_tag="ACL8A1_001154"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HGS1201935.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAVLKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            23216..24541
                     /locus_tag="ACL8A1_001155"
     CDS             23216..24541
                     /locus_tag="ACL8A1_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGS1201936.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGILIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGALYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFRTVLYLPQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            24572..24748
                     /locus_tag="ACL8A1_001156"
     CDS             24572..24748
                     /locus_tag="ACL8A1_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HGS1201937.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            24807..24941
                     /locus_tag="ACL8A1_001157"
     CDS             24807..24941
                     /locus_tag="ACL8A1_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HGS1201938.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            25005..26315
                     /gene="obgE"
                     /locus_tag="ACL8A1_001158"
     CDS             25005..26315
                     /gene="obgE"
                     /locus_tag="ACL8A1_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HGS1201939.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLAENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            26325..26483
                     /locus_tag="ACL8A1_001159"
     CDS             26325..26483
                     /locus_tag="ACL8A1_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201940.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            complement(27358..27483)
                     /locus_tag="ACL8A1_001160"
     CDS             complement(27358..27483)
                     /locus_tag="ACL8A1_001160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201941.1"
                     /translation="MKIKDQTRKLAAGCSKHCFEVVDGTDVVSILHIRQGDIDVI"
     gene            27626..28885
                     /locus_tag="ACL8A1_001161"
     CDS             27626..28885
                     /locus_tag="ACL8A1_001161"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HGS1201942.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            28901..29452
                     /locus_tag="ACL8A1_001162"
     CDS             28901..29452
                     /locus_tag="ACL8A1_001162"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1201943.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENKASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            29445..30722
                     /gene="spxR"
                     /locus_tag="ACL8A1_001163"
     CDS             29445..30722
                     /gene="spxR"
                     /locus_tag="ACL8A1_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS-HotDog domain-containing transcription
                     factor SpxR"
                     /protein_id="HGS1201944.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            30738..31598
                     /locus_tag="ACL8A1_001164"
     CDS             30738..31598
                     /locus_tag="ACL8A1_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HGS1201945.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            31823..31939
                     /locus_tag="ACL8A1_001165"
                     /pseudo
     CDS             31823..31939
                     /locus_tag="ACL8A1_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase"
BASE COUNT        10291 a         5155 c         7004 g         9573 t
ORIGIN      
        1 aatactatgt caaaagaaaa aataaaagta tacctctata cacgagtatc tacgtcaata
       61 cagatagagg ggtattcgtt agaggcacaa aaatcacgaa tgaaagcttt tgctatttac
      121 aacgattatg aaattgttgg agagtatgaa gatgccggga agtctggcaa gtctattgaa
      181 ggtagaatac agttcaatcg aatgatggaa gatataaaat ctggaaaaga tggagtatct
      241 tttgttcttg tgtttaagtt atcaagattt gcaagaaatg ccgctgatgt tctttctaca
      301 cttcagataa tgcaggatta tggagttaat cttatctgcg ttgaagatgg cattgattca
      361 tctaaagatg ctgggaaact aatgatttct gttttatcag ctgtggctga aatagaaaga
      421 gaaaacatcc gtattcaaac aatggaaggt cgcattcaaa aagcaaggga aggaaaatgg
      481 aatggtggct ttgctccgta tggatataaa cttgaagacg gcaagctgtt tataaatgag
      541 gaagaggcag ttgcaataag aacgattttc gaccagtatg taaatactac gataggagcc
      601 aatgggatat ctaaatactt agagaatcat ggaattagaa aaatcccaag acagaatggt
      661 aagaatcctt tgtttgatgc aggtcttata agaaagatat taaagaatcc tgtatataat
      721 gggaaaatag catttggaag aagaacttta gaaaaagttc atggtacaag aaatgaatat
      781 aagcagcttg aacaagatga atatctaata tctgaaggga tacatgaagc tatagtttcc
      841 gatgaagttt ggtaagctgc tcaggttaag ctaaaatctc aagcaaagaa atatgagcat
      901 gtgaataaag gaaaagatac acgcacacac ttgctttctg gaattgtaaa atgcccgata
      961 tgtggagtgg gaatgtttgg gaacaagtgt atcaagaaaa agaaagatgg cacaaagtat
     1021 aaagattttt attactatgg ttgtaaacat aggcagatga taagaggtca taagtgtaca
     1081 ttcagtaagc aaattagaga agaattgtta gatgatgctg ttgcagaggt gattgtcaag
     1141 atagtaagta atccgaaatt tgcttctatg atgcaagaaa aaatcaacat gaaggtggat
     1201 acctctgaaa tagaaaaaga aatagataat taccaaaagg aattgagaaa gagtcattct
     1261 acaaagttta agctaattga ggaaatagat aatttagatg ttgaagataa gcattataag
     1321 agaaggaaac aggatttaga tgatagacta tatcgtatgt atgacaaaat agatgaatta
     1381 gaatcatcac taattgatgc gaaagcaaag aaacagacta ttgaagctga aaagcttaca
     1441 ggagataaca tatataaggt tctgatctat tttgataagc tctataaagt catgaatgat
     1501 gtagagcgta ggcagttaat ttcagctttg atttctgaaa ttcaagttta tgaagaaaaa
     1561 caatctaacg gacaatggct aaaatccatt acttttaagc taccaattat agaagaaaat
     1621 ctcaacatag gtttggacaa tggtgaacaa gttgaatgtg taagtttatt agagaaacgt
     1681 agttaatcct caaaaggttc cccgaacctt ttgagtccca ctgacgcatc acgctgaggc
     1741 ggtatcgctg tttgtacgag ttgaagcatc agcaaagtaa aagtagatgt tcagccaatg
     1801 cgacgaggtg agaccgaagg tcgataaggt gtgttctggg tataggactc gtaaagtaag
     1861 taggaaatat gatgagatga tacagagagc ctgaattggt caggtgacgt gcttatttac
     1921 attagcctga taatgaaaat ggagagatta tcgatggtta tattggaatt gtaccaaaat
     1981 gattattcta aagatttagt agcatttgat tccatagaag atgggaaagc ctttgtagct
     2041 caaatcccgg gatacactct ggaaacagaa gatggtgttg aagtggagta ttttaatcct
     2101 aaaaacatac cggattatat ggaaattatt tttaacggaa atattgttcc tttatctaga
     2161 tttatgttcg atcctgggga aaatgtaaac attatttgga aagagatttc aaatttatcc
     2221 ctcaaaaatg atagagtgat cgaaggatat tcaaaaattg atgcatatgt cgttaacaac
     2281 catgaagtta aagcctatgt cgaaacaaga gaaacgaact atcgtaaagc aaaagacttc
     2341 ttagaaagcc gcggatatga aatcgacaga agctttttcg gaagtgaaga cggtgaagcg
     2401 attctttaca gaaaaaaagg gatcgaagtt tggcatttct tgtgtcactt agacccaatg
     2461 tttgtagaga ttgaagatgt agaaggatat gttaaagaag aagttggaga gattcaatag
     2521 tcaaccaaag ttccttgtga gtatatacct ttgctagtaa aagagtcaaa acgttataac
     2581 gaaggggggg aagaaaaccc ttgagtgtaa gtctttttct tgtttagagg gaaagtatta
     2641 gagtagccgc tcataattta tactagattt gataactgat ttgagacaga ttttatttct
     2701 ataaatattg tgaaaatttt ttaataccaa agatttaaag ttactgtgtt aaaaatcctc
     2761 tccactttta aactaatgta ttataaaagc cagttgacat aagtgttaca cctgtgatag
     2821 aattaacttg aggtgatgaa aatggcaacc gataaaaata gaataatgat tagcttggat
     2881 gataaaaatc tagagaaact tgaaaattta gtagaagatg ctagagatag aaggggaatg
     2941 cgactaacaa agtcccaggt tattgaatta ctcctaaata ccgttgatta tttcgatgat
     3001 attatggggg ctatttactc aaaaaaataa gagcaaaatt gctattattt tttacaaatt
     3061 ggtgtaacac tagtgatata ggagtgatga aatggttgtt tatattagac aaagtaaatt
     3121 accaagtgag gtttcaatta ataaatacaa tgctcaagta ggtgcctact tacaaggaga
     3181 agaagctgtt ttatatcaat ctttttctga aataaaagag ttaacaagtg aagatatagt
     3241 tgtagattat ataatggaga ctagagcatt actaaaaatg atgggcttaa acgttccggt
     3301 tcatgactat cctattgagc ttaaagagtt ttatggtcga aagatttatg ctggtatttt
     3361 aggagagatt gtgaatatac ctgataattg gggaaaattt attaagccta aagctggttc
     3421 aaaagtcttt actggaagag ttgttaatgg aacccatgat ttaataggta ttggtctacc
     3481 tttcgactat cctatatgga ttagtgaggt tgtagaattc atagctgaat ggcgttgttt
     3541 tgtgttagat ggtcgcgtat tagatgttcg accctataca ggtgattatc atgcacaatt
     3601 tgatgcaagt gtaattgatg aagcgatatc atgttggaaa gatgcgccaa tagcttatgg
     3661 actagatatc ggtgttactc gcgatggcag aacacttgtt gttgaagtaa atgatggtta
     3721 tgcattggga aattatggcc tatttccttt aaaatcaatc aattttcata gagctaggtg
     3781 gaaagaaatg gtaaaaccct attttgaaaa aaatgaaatt tttaaaatcc aacaggatgt
     3841 tattttctaa aataaatatg gtagataacc aatataaaaa gcatcaaaat gaacaaggag
     3901 gaatctgatg cttggagcaa ttattggaga tattgtcggt tctgtttacg aatggaacaa
     3961 tatcaaaaca aaggactttc ctttatttcg gaaggactgc ttttttacag atgacacggt
     4021 tatgacctgt gctgttgcag aagcaattat gaatggtgga caaaaagacg acttcattga
     4081 cgcgatgaag aaatatggca gaatgtatcc gaatgctgat tacggtgctc ggtttaatgc
     4141 atggctaaac agcgataatc gtgagcctta taatagcttt gggaatggat cagctatgcg
     4201 tatttctcca tgtgcttgga tcatggactg tggtttttat gcgaaaactg gtatatggcc
     4261 atcatctaga ggacttacga gtctttctgc agaggtaact cataatcatc cagaaggtgt
     4321 caaaggcgct atggctacag ctgatgctat ctttctatgt cgtttttact ttggtggtta
     4381 ttgtagagag tacgagcaat caattaacga caatcctaca gagtgtaaaa gacgaattaa
     4441 ggattatata gaaaaagaat acgattacaa tctatctcaa actttagatg aaatccgtcc
     4501 taactatcgt tttaacgaaa catgtcagga aactgtccct caagccatta tcgcctttct
     4561 tgaaagtaga gacttcgaag atgcgataag aaatgccatc tcacttggtg gcgacagtga
     4621 tactctcgca gcaatcactt gtagcatagc agaggcagct tacggtattc ctgattggat
     4681 caaggataag gcctattctt acttggatga acccttaaag gatgtagtta ggcgatggga
     4741 aaatagaata aaagcgtatt aatctccagt acgtcagatt tctcttttct gtattttcat
     4801 tgtataatag gattacattt acttgcatac gacgaaatgt aaacatttct gtttaaaatt
     4861 aaggataaaa tcaatgaaaa aagcaatggt aattatcaac cctacttctg gtggcgagaa
     4921 ggctttggat tacaaagaaa agctggagaa taaagcaaaa gaatactttg aatatgttga
     4981 aaccaaaatt accgaaaaag cgctggatgc aacacatttt gctgaagaag cttctcgtga
     5041 gcagtatgat gcagtggttg tgtttggtgg agatggaact gtcaatgaag tcatttcagg
     5101 tattgatgag agagactaca ttcctaagtt agggattatc ccaggcggta cgggtaacct
     5161 cattacaaaa cttttggaaa tcaatcaaga catcgatggc gcaattgacg aactggattt
     5221 tgatttaacc aataagattg atatcggtaa agcaaatgac aactattttg gttatatctt
     5281 tagtatcggt tctctgcctg aggcgattca caatgttgaa atagaggaca aaacaaaatt
     5341 cggtatttta acctatgctg taaataccat gaagtctgtc atgacagatc aggtctttaa
     5401 cattaaggtt gagacagaaa atggaaatta tgttggtgaa gctagccatg ttttggtcct
     5461 tttgacaaat tacttcgctg ataagaaaat ctttgaagaa aacaaggacg gctatgccaa
     5521 cattttgatt ctgaaagatg cctctatatt ctccaaatta tccgtcattc ctgatttatt
     5581 aaaaggggat gttgtcgcaa atgataatat cgagtatatc aaagcgcgta atattaaaat
     5641 ctcttcagat agtgaattgg agtcagatgt tgacggagat aaatcagata acctacctgt
     5701 agaaatcaaa gtcctagctc agcgagtaga agtattttca aaaccgaaag agtaagtttg
     5761 tcattcactg agaaataaag ttttatatca acgaaagtag ttacttgtaa gatttgctaa
     5821 attttgtaaa attaacatgt cgtttaaaat aattggataa tgggtttata taaaaatcag
     5881 gagaagttag aagaaaaaat gaaagacaaa gaaagttata ataaatttat tccaaagtta
     5941 tacaatgaag aacttttaaa gaatcatttt aaagaaacta tgtgtttttc taggaaggaa
     6001 ttaaataata agagtataat aaatcaaaag ctcattaaat tccccatagt tggtcatgaa
     6061 gtttgggcat tatttggtaa agaaaaaacg ggcgaatggc ggtgtttaca agttggacaa
     6121 agtaaaaata aagtaaaggc cgaaattgaa tcattaattg aatttatgtc ttatgattat
     6181 aatcaattag ttgatagtat taaagatggt gtaaaaaata gaaatagtac tttttatagt
     6241 aatatttatc aatcttctaa aaaaagcaag aatgaatttt tatacagtca tatagcaagc
     6301 caatacgatg aatttcaact ttgtctacta gatattgata aatatcttgg ctttaaaaac
     6361 ttaaaatttg aaaataaaca tattagtaat ataatgaaaa ttgcaaaacc attgtatgca
     6421 gaggctaaac ttgcatttga aacaaaatct atatattgga ctatgtataa ttctggagtt
     6481 gatggacaag caatcatgat ttttttagga gataacaaag attaattaaa tgtcttcttt
     6541 tctatcttca aatatatact actatcttga gtctatatga aattggaatt caaaattact
     6601 aattaaattt gaatagattg taccatactt gtaaacttta tatccttcta atcaatagta
     6661 gtaattcaaa gcctttttta aggctttttg tatactttaa aagatagttc ctttaacaac
     6721 ggacattcct tgcaaatagt ttacaaaaat agtatactgg attcattgag tttgaaaacg
     6781 tttgcgtaaa atttgaatga atactttagg agacaaattg atggaattga gtgctattta
     6841 ccataggcct gagtcggagt atgcctatct ttataaggat aagaaactcc atattcgaat
     6901 tcgaactaag aaaggggaca ttgaaagcat caacttgcac tatggggacc cttttatctt
     6961 tatggaggag ttttatcagg atacaaaaga aatggtcaag ataacttctg gtaccttatt
     7021 tgaccattgg caggttgaag tgtcagttga ctttgcacgt atccagtatc tctttgagct
     7081 cagagataca gaaggtcaaa atattttgta tggcgataaa gggtgtgtgg aaaattctct
     7141 agaaaatctt catgcaattg ggaatggatt taagttgcct tatcttcatg agattgatgc
     7201 ctgcaaggtt cctgactggg tttcaaatac ggtatggtat cagatatttc ctgaaagatt
     7261 tgccaatggc aatgctctat taaacccaga agggacttta gactgggatt catctgtcac
     7321 acctaagagc gatgatttct ttggtggtga tttacagggg attattgatc atatggatta
     7381 cttgcaagac ttgggtatta ctggactata tctttgtccc atctttgaat ctacaagcaa
     7441 tcacaagtac aatacgacag attactttga aattgaccgt cattttggag acaaggagac
     7501 ctttcgggaa ctggtggatc aagcgcatca tcgtggcatg aaagtcatgc tggatgcggt
     7561 atttaatcat attggttcgc aatctcttca atgaaaaaat gtcgtcaaaa atggtgaaca
     7621 gtctgcttat aaggattggt tccatattca acaattccca gtgacaactg aaaagctagt
     7681 taataagaga gacttaccct atcatgtttt tggtttcgag gactatatgc ctaagctaaa
     7741 tacagccaat ccagaggtca agaattatct tttaaaggtt gcgacttatt ggattgaaga
     7801 gtttaatatc gatgcttggc gtttggatgt ggctaatgag attgaccatc agttctggaa
     7861 ggattttcgt aaggcagttt tagctaaaaa tcctgatctt tatatcctag gagaagtctg
     7921 gcatacatct cagccttggc taaatggaga tgagttccat gccgtcatga attatccttt
     7981 atctgatagt atcaaggact atttcttacg aggaattaag aagacagacc agttcatcga
     8041 tgaaatcaat ggagagtcta tgtattacaa gcagcagatt tcagaggtca tgtttaatct
     8101 cttggattca catgatacag agcgaatcct gtggacggcc aatgaagatg ttcaactggt
     8161 taaatcagcc ttagcctttc tctttttaca aaaaggaaca ccgtgcattt attacggaac
     8221 cgagctagcc ttgactggag gaccagatcc agattgtcgt cgttgtatgc cttgggaacg
     8281 tgtatcaagt gacaatgata tgctgaactt tatgaagagg ctgattaaaa ttcggaaata
     8341 cgcgtcagta atcatttcgc atggcaagta tagccttcaa gaaatcaaat ctgatctagt
     8401 agctctggaa tggaaatacg aaggacggat cctcaaagta atattcaacc aatcaacaga
     8461 agattatctt ttagagaaag aagcagtagc actagcaagc aattgccaag aattggagaa
     8521 tcagcttgtc atctctccag atggatttgt gattttctaa aaactagttg atgaagatta
     8581 tggtacattt catatcttat atagtataat aaggctagtt actaaacttg taaaggagaa
     8641 cttaaatgaa ttgtagagga catgaaacaa gacaaagaat tgttagagat tttgaagttt
     8701 agcctaaagc acatattaag ctgttagcaa atcaacaaaa acatagtgat gcaggagcaa
     8761 ctattgaaga tgaatattat gtatttatcg ctgagagtaa aattgatggc aagaaggaag
     8821 ttattcagtg ttgcatgggt gcggcaaggg attttttaga actaattaat cacaaagggc
     8881 tacctctttt taatccgctt gtaggtgatt ctcatgtaaa taatagacaa gaatatgaca
     8941 atacagggag tggaaattta caacctgaaa agtggaatga aactgcaaag cagctttata
     9001 atattataat gtggttgatt attttatgga atgctaagcc ggatacacct ttatttaatt
     9061 ttaaagacga agtaattaag tataaaacat atgagccttt tgaaagcagt ataaaaagag
     9121 taaatactac tataaagaat ggtagtaaag ggaaaactct gactgagatg attaatggct
     9181 acagagcgga taacgatatt agagatgaaa tttgtaactt taatattctg aaaaataaaa
     9241 ttcgtgatat gaaagaccaa caaggaaata caatggaatc ttacttttag ttattgttga
     9301 attttgggta ttctataaaa tatcctaatt gagatttaaa tagtagacta tacaatatag
     9361 ttaaaatatc agtaaaaaca acactttatt gaggtattgg atacgctttg ctaatagcct
     9421 aataatcaca tgtggagtgt tgctacaacg aaaaaggtga taatccttga tttcaagcta
     9481 ttttataagc attttgtctt tgtagataaa ggcaattttg acaataaaaa tcctaaaagg
     9541 tgaatcgtta tagatgtatt tgtagatatc gtttgcgcat cgaaaaaatt aatacaagaa
     9601 taaatattta tggctcttta ggtgactttt atagaagtaa agtttaggat agaaaaacaa
     9661 gaaataacgc accatttttg gtgcgttatg ctttttgttg tctcccgaaa accactagct
     9721 atgctagtgg ttccatagag cttttagcgt ggtaacaaaa agaacctcct aaaatgataa
     9781 gatagaagtg gtttctccgc cactacaaca tatcatacag gaggtacctc atgagagagg
     9841 ataatcaaag tttatcacat accacatgga attgtaaata tcatattgtt tttgcaccca
     9901 aatatcgtcg tcaaatcatt tatggcagat acaaagctag tatcggaaga atcatacgtg
     9961 acttatgtga gcgtaagggt gtaataatcc atgaagcgaa tgcttgttca gaccatattc
    10021 acatgcttat cagtattcct ccgaaactta gtgtttcgtc ctttatgggc tatttaaagg
    10081 gcaagagcag tttgatgatt tttgataagc atgcgaattt aaaatacaaa tatggcaatc
    10141 gcaagttttg gtgtagaggc tattatgtag atacggtagg ccgtaatcag aaagtgatag
    10201 ctgaatatat tcagaatcaa ttacaagaag acagagtagc agaccagctc acgttattcg
    10261 agtcagtaga tccgtttact ggcgaaataa ataagaggaa gtaactaagg tgctttagca
    10321 cctgctcggg aaagtggtgc gcgaggaagc tatttcggtg ggcctttggc cctggccggt
    10381 agaagcggct tatagccgca gaacaaacca ccagttcaca ctggtggttt tgatttaaaa
    10441 aacttgatac ataaaaataa aagtctatat aaaggatggt aaaattcctg ttgtccgatt
    10501 tggacaatat cctaaatagt tacaatatat ggtctatact tttctcagga gaaagctaga
    10561 tgtacagacg tttgagagat ttgagggaag atcacgattt gacccaaaaa caaatagcta
    10621 aaatactttc gtttacaaac tcagcttatg ctaaaattga acggggtgag catacgttaa
    10681 cagcagatgt attggtaact ctctcaaact tttacgatgt cagtacagac tacctattgg
    10741 aattgacaga ttttcctgat aaaattcgct ttagaaaata atctcctcaa tttcatagag
    10801 tgtgaaaatg aggagatttt ttatttgttc tttgaaaatt aaatagtcca aaatggtact
    10861 ttcctcattt gtggagcaaa tttgaatggc tcgccatgat aagagcgatt ttaaaaccat
    10921 caataaaata gagcgatact ttatatgcca tgatacaaat gatatacaat gatacttctg
    10981 accgttcagc ctgccaacgt aaaagagcag caagtgaaat tcttatgatg acttcatcag
    11041 tcatgccacg ttgaatgtgt gagtttgtta gataaacgca attaatcctc aaaaggttcc
    11101 ccgaaccttt tgagttctac agacgcatca cgtggagtgt gtaagcttgt tgctaaaagc
    11161 gtaaaaacct tggaacgaaa ggaataatag acttcctgcg aaacaaaaat ataatacaat
    11221 aaaactatga atgatgaagc aagtaaacaa ttgagcgata gccgtttcaa gatccttgta
    11281 ggtgttcagc gcacgacttt tgaagagatg ttagctgtgt taaaaacagc ttatcaacgt
    11341 aaacgcgcaa aaggtggacg aaaaagcaaa ttaagcctag acgatctcct tatggtaact
    11401 attcaataca tgcgagaata gagcacttat gaacaaattg cggctgattt tggcattcac
    11461 gaaagcaact taatccgtcg gagtcaatgg gttgaagcaa ctcttattca aaatggtttt
    11521 acgatttcaa attctgcctt aattctgtaa aaacagtaaa attcgaagga ttgtaaggta
    11581 agagtttttt tctttctgaa aaaatggtat aatagcaatc aaaactagaa aataaaacgg
    11641 aatttggaac agatttgtct gtatcctagt agagtggtga tactatgaag attagtaaga
    11701 ggcacttatt aaattattcc atcttgattc cctacttgct tttatctatt ttgggcttga
    11761 ttgtggtcta ttcgaccacc agtgctattt taattgaaga aggcaagagc gccttgcagt
    11821 tggttcgaaa ccaaggaatc ttttggattg ttagtttgat actgattgcc ttaatttata
    11881 aattgagact agattttttg agaaatgagc gactaatcat tttagttata ttaatagaaa
    11941 tgcttttatt gttcttggct cgttttattg gtatttcagt aaacggggca tacggttgga
    12001 tttcggttgc aggagtaact attcagccag ctgagtactt aaaaatcatt attatttggt
    12061 atttagctca ccgattctcc aaacagcaag aagaaatagc tacttatgat tttcaagttt
    12121 tgactcaaaa tcaatggctt ccccgtgctt ttaatgattg gcgattcgtt ctcctagttc
    12181 tgattggaag tttgggaatt ttccctgatt taggaaatgc gactatttta gtcttggttt
    12241 ccttgattat gtatacagtt agtggaatcg cttatcgctg gttttcaacc attctggcgc
    12301 tcgtatctgc cgcttctgtc tttgtcttga ccactatcag cctaatcggt gttgagacct
    12361 tttcaaaaat tccagtattt ggctatgtag ccaaacgctt tagtgccttt tttaatcctt
    12421 ttgccgatcg tgctgatgca ggtcaccagt tagctaattc ttattttgcc atggtcaatg
    12481 gtggttggtt tggtctaggt cttggaaact cgattgaaaa acgaggttat ttgccagaag
    12541 ctcatacaga ctttgtcttt tctatcgtga ttgaagaatt tggctttgtt ggtgccagtc
    12601 ttattttagc tctcttgttt ttcatgattt tgcggattat cttggtcggt attcgagcgg
    12661 agaatccttt caatgccatg gttgcactcg gtgtcggagg gatgatgttg gttcaggtat
    12721 ttgtcaatat cggagggatt tcgggcttga ttccatctac aggagtaacc ttccccttct
    12781 tatcccaggg tggaaatagt cttctagtct tatcagtggc agtagccttt gtcttaaata
    12841 ttgatgccag tgaaaaacgc gctaagttgt accgagaatt ggaaaatcaa ccaatgaacc
    12901 gtctgttgaa gtaggataaa gaaaggatag tttatgtctc ttcaaaaatt agaaaattct
    12961 agtaataaaa gtgttgtgca agaagaagtc ttgattctaa cagaattact ggaagatatt
    13021 actaaaaata tgcttgcccc agagaccttt gaaaaaataa tacagttgaa agaattatca
    13081 acgcaggaag attatcaagg tctaaaccgt ctagtgacta gcttatcaaa tgatgaaatg
    13141 gtctatattt cacgctattt ctctatcttg cctcttttga ttaatatttc agaggatgtg
    13201 gatttagctt atgaaatcaa tcatcaaaat aatattgatc aggactattt aggtaaatta
    13261 tctacaacga ttaaattggt agcagaaaag gaaaatgccg ttgagatcct agaacacttg
    13321 aatgttgttc cggttttgac agcccatcca acacaagtgc aacgcaaaag tatgttggat
    13381 ttaacaaatc atattcatag tcttttgcgt aaataccgtg atgttaagtt ggggttgatc
    13441 aataaagata aatggtacaa tgatttgcgt cgttacatcg aaattatcat gcagacagac
    13501 atgattcgtg agaaaaaatt aaaagtgact aacgaaatca cgaatgctat ggaatattat
    13561 aacagctcct ttttgaaagc tgtacctcat ttgacgacgg agtataagcg cttagcgcaa
    13621 gcgcatggtc tgaatttaaa acaggctaaa ccaatcacca tgggtatgtg gataggtggt
    13681 gaccgtgatg gaaatccatt tgttacagca aagaccttga agcagtctgc actcactcag
    13741 tgtgaagtca tcatgaacta ctatgataaa aagatttacc aactttatcg tgaattttct
    13801 ctttcaacta gcattgtcaa cgtcagcaag caagtcagag aaatggctcg tcaatccaag
    13861 gataactcga tttaccgcga aaaagagctt taccgtcgtg ccttgtttga tattcaatca
    13921 aaaattcagg caactaaaac ctatctgatt gaggatgaag aagttgggac tcgttatgaa
    13981 accgccaatg atttctacaa ggatttgatt gccattcgag attctctact agaaaataag
    14041 ggcgagtcct tgatttcagg tgattttgtg gaattattgc aggcagtaga gatatttggt
    14101 ttttacttag catcaattga tatgcgacaa gactctagcg tctatgaagc ctgtgtggca
    14161 gaactcttga aatcagcagg aattcattct cgttatagcg agttgagcga agaagaaaag
    14221 tgtgaccttc tcttgaaaga attagaagaa gatccccgaa ttctttctgc gactcacgca
    14281 gaaaaatcag aattattagc aaaagaacta gctattttta agacggctcg tgttttgaaa
    14341 gataagttgg gagatgatgt catccgtcag accatcattt cacatgcaac cagcctttct
    14401 gatatgctag aattagctat tctgttaaaa gaagtaggac tggtggatac ggaaagggcg
    14461 cgtgttcaga ttgttcccct ttttgaaaca attgaagact tggatcattc agaggaaaca
    14521 atgagaaaat atctttctct tagccttgcc aaaaaatgga ttgactcacg aaataactac
    14581 caagaaatca tgcttggcta ctctgacagt aataaagatg gcggttactt gtcatcatgt
    14641 tggaccctct acaaggctca acagcaattg actgctattg gagatgaatt tggcgttaag
    14701 gttaccttct tccatggtcg tggtggtact gtcggtcgtg gtggtgggcc aacctatgaa
    14761 gccattacat ctcaaccgct caagtctatc aaggatcgta tccgcttgac ggagcagggt
    14821 gaagtaattg ggaataaata cggtaacaaa gacgccgctt actataacct tgaaatgcta
    14881 gtatcggcag ctattaaccg tatgattact cagaagaaga gtgataccaa taccccaaat
    14941 cgttatgaag ccattatgga tcaagtagtg gaccgtagtt acgatgtcta ccgtgatttg
    15001 gtctttggta atgagcattt ctatgattat ttcttcgagt caagtccaat caaggctatt
    15061 tcaagtttta atattggttc tcgtccagcc gctcgtaaga ctattactga aatcggtggt
    15121 ttgcgtgcca tcccttgggt attctcatgg tcacagagtc gtgttatgtt ccctggatgg
    15181 tacggggttg gttcaagctt caaggaattt atcaataaaa atccagagaa tattgctatc
    15241 ttacgagata tgtaccaaaa ttggcctttc ttccaatcgc ttctttcaaa tgttgatatg
    15301 gttttgtcaa aatcaaatat gaatattgct tttgaatatg ctaaactttg tgaagacgag
    15361 caagttaagg ccatctatga gattatttta aatgaatggc aagttactaa gaacgttatc
    15421 ttggctattg aaggacatga cgaactctta gctgacaatc catatctaaa agctagtctg
    15481 gattaccgta tgccttactt taatattctc aactatattc agttagagtt gattaaacgc
    15541 caacgtcgtg gagaattgtc cagtgatcaa gaacgattga ttcatatcac catcaacgga
    15601 attgcgacag gattgcgtaa ttcaggttga taattttcaa gagtgaatgc taaaagtgaa
    15661 tatcaaaaaa attctaatag actattgaca agtagtttaa aaatgatata atttaaccat
    15721 tcagaaaagt aatcatacaa actttttaga gagtctgtgg tagctgaaaa cagataagtg
    15781 gcaatgatga aaattgggct gaatgctatt tagaatttga aattataaaa attcggtaag
    15841 cacaccttac agtgcatctc gttattgcga gactgagcga tagggaaatt ccctataatt
    15901 gaggtggtac cgcgcatcga cgtcctcaca caagtttttt gtgtgaggat ttttttgatg
    15961 gaggttagta tggaaagaaa acgatggcgt cgcttgttta gataagtgaa atatgttaaa
    16021 ggaaataaaa aggagaaaca gaatgaaaaa taaacgttta attggaatta ttgctgcatt
    16081 agcagtctta gtagcaggaa gcttgattta ttcttcaatg aataaatcag aagctcagaa
    16141 taataaggat gagaagaaaa taaccaagat tggtgtgctt caatttgtga gccatccatc
    16201 ccttgatttg atttataaag ggatccaaga tggacttgca gaagaaggat ataaagatga
    16261 tcaagttaaa attgatttta tgaactcaga aggtgatcaa agtaaggttg cgacaatgag
    16321 taaacaattg gttgcaaatg ggaatgacct tgtggttggt atcgcaacac cagcagccca
    16381 agggttggct agtgcaacaa aagacctacc agttatcatg gccgctatta cagacccaat
    16441 tggtgctaac ttggttaaag atttgaaaaa accaggtggc aacgttacag gggtatctga
    16501 ccacaatcca gctcaacaac aaattgaact catcaaggct ctgacaccga atgtgaaaac
    16561 aatcggagct ctttactcaa gtagcgaaga caattcaaaa acacaggtcg aagaatttaa
    16621 ggcttatgct gaaaaagcag gtctgacagt ggaaacattt gcagttcctt caacaaatga
    16681 aattgcctca actgtcactg ttatgactag caaggtagat gctatttggg ttccaattga
    16741 taacaccatt gcatcaggat ttccaacggt tgtctctagc aatcaaagtt ctaagaaacc
    16801 aatttatccc agtgcgacag ctatggtaga agtaggtggt ttggcatcag ttgtaattga
    16861 ccaacatgac cttggtgtgg caacaggtaa aatgattgtg caagtcttga aaggtgcaaa
    16921 accagccgat accccagtca atgtcttttc aactggtaag tcagtcatca ataaaaaaat
    16981 agcacaagaa ctaggtatta ctattcctga gtctgttctc aaagaagcag gacaagtcat
    17041 cgaataaata aggtagggag gaggagactc ttcccatttt ttaaaaagat gaatatagaa
    17101 aggattaaat aaaatatgat tgtttccatt atttctcaag gatttgtctg ggctattcta
    17161 ggtctgggaa tctttatgac atttaggatt ttaaactttc cagatatgac gacagaaggt
    17221 tccttccctc ttgggggagc tgttgctgtc actttgataa ccaaaggcgt gaacccattt
    17281 ttagcgacac ttgttgctgt aggagcaggt tgtttggctg gaatggcagc aggccttctt
    17341 tatacaaaag ggaagatccc aaccttgctc tcagggattt tggtgatgac ttcttgtcac
    17401 tcaatcatgc tcttgattat gggacgtgcg aatttaggcc tgcttggaac caagcaaatt
    17461 caggatgttt tgccttttga ttcggatttg aatcaactct tgacaggtct catctttgtg
    17521 agtattgtta ttgctctcat gctctttttc ttggacacta aactcggaca agcctatatt
    17581 gctacagggg ataatcctga tatggctaga agtttcggga ttcatactgg acgcatggag
    17641 ctcatgggct tggtcttatc aaatggtgtg attgcccttg caggtgccct cattgctcag
    17701 caagaaggct atgccgatgt gtctcgaggg atcggggtta tcgttgtggg gcttgcaagt
    17761 ttgattattg gagaagttat tttcaagagt ttgagcttgg cagagcgttt ggttactatc
    17821 gttgtaggtt ctatcgctta tcaattttta gtgtgggcag ttatcgcact tggctttaat
    17881 acaagttacc ttcgtttata cagtgccttg attttagcag tctgcctcat gattccaaca
    17941 tttaagcaaa caatcttgaa aggagccaag ttaagcaaat gacagcaatt gtagaattaa
    18001 aaaatgcaac caaaatcgtt aaaaatggct ttgatgaaga aaagattatt ttaaatgatg
    18061 tttccttaga aatttttgaa cgggacttta tcacgatttt gggcggaaat ggtgctggaa
    18121 aatcaaccct ctttaacact atagcaggga ccttatcact aactagtgga actatccgta
    18181 ttttaggtga agaactcact aagttttcac ccgagaagcg tgccaagtac ctgtctcgtg
    18241 tcttccaaga tccaaagatg gggacagctc cccgtatgac ggtcgctgaa aatcttttaa
    18301 tcgccaagtt tcgtggtgaa aagcgtggat tgttaccacg acgcttgact agctataagg
    18361 atgaatttca ggcaaccatt gaaaaagtag gaaatggtct tgagaaacac ttgaatacac
    18421 cgattgagtt cttatcaggt ggacaaagac aggctttgag tctcttgatg gcaaccttga
    18481 agcgacctga attactcctg ttagatgagc atactgctgc cctggatcca aagactagtg
    18541 ttgctttgat ggaattgaca gatgaatttg ttaagaaaga tcagctaaca gcccttatga
    18601 ttactcatca tatggaagat gctctcaaat acggcaatcg cttgattgtc atgaaagaag
    18661 gacggattat ccaagattta aaccaagaag aaaaagcaaa aatgaaaatc tctgattatt
    18721 atcaactctt tgaataaagt agggaaaatg agtgaaatgg tttacttttt ttctgaaata
    18781 aagtatacta tataaagtaa actatgataa catggaggta ttgtgtatgg ttgacaaaca
    18841 agtcattgaa gaaatcaaaa acaatgccaa cattgtggaa gtcataggag atgtgatttc
    18901 tttacaaaag gcaggacgga actatctagg gctctgtcct tttcatggtg aaaaaacacc
    18961 ttctttcaac gttgtagagg acaagcagtt ttaccactgt tttggttgtg gtcgctcagg
    19021 tgatgtcttt aaattcatcg aggagtacca aggggttacc tttatggagg ctgtccaaat
    19081 attaggtcag cgtgtcggga ttgaggttga aaaaccgctt tatagtgaac agaagccagc
    19141 ctcgcctcac caagctcttt atgatatgca cgaagatgcg gctaaatttt accatgctat
    19201 tctcatgaca acgactatgg gcgaagaggc cagaaattac ctttatcagc ggggtttgac
    19261 agatgaagtg cttaaacatt tttggattgg tttagcacct ccagaacgaa actatctcta
    19321 tcaacgtttg tctgatcagt atcgtgaaga agatttactg gattcaggcc tgttttatct
    19381 ctcggatgcc aatcaatttg tagacacctt tcacaatcgc attatgtttc ccctgacaaa
    19441 tgaccaagga aaggtcattg ccttctcagg tcgtatctgg caaaaaacag attcacaaac
    19501 ttctaagtat aaaaacagcc gatcgactgc aatttttaac aaaagttacg aattatatca
    19561 tatggatagg gcaaaaaaat cttctggaaa aactagtgag atttacctga tggaaggatt
    19621 catggatgtt attgcagcct atcgggctgg aatcgaaaat gctgtggcgt cgatgggaac
    19681 ggccttgagt cgagagcatg ttgagcatct gaaaaggtta accaagaaat tggttcttgt
    19741 ttacgatgga gataaggctg ggcaagccgc gatattgaaa gcattggatg aaattggtga
    19801 tatgcctgtg caaatcgtta gcatgcctga taacttggat cctgatgagt atctacaaaa
    19861 aaatggtcca gaagacttgg cctatctatt aacgaaaact cgtattagtc cgattgagtt
    19921 ctacattcat cagtacaaac ctgaaaacag tgaaaatctg caggctcaga ttgagtttat
    19981 tgaaaaaata gctcccttga ttgttcaaga aaagtccatc gctgctcaaa acagttatat
    20041 tcatatttta gctgacagtc tggcgtcctt tgattatgcc cagattgagc agattgttaa
    20101 tgagagtcgt caggtgcaaa ggcagaatcg catggaagga atttccagac cgacgccaat
    20161 caccatgcct atcaccaagc agttatcggc tattatgagg gcagaagccc atctactcta
    20221 tcggatgatg gaatcccctc ttgttttgaa cgattaccgt ttgcgagaag actttgcatt
    20281 tgctacacct gaatttcagg tcttatatga cttgcttggc cagtatggaa atcttcctcc
    20341 agaagtttta gcagagcaga cagaggaagt tgaaagagct tggtaccaag ttttagctca
    20401 ggatttgcct gctgagatat cgccgcagga acttagtgaa gtagagatga ctcgaaacaa
    20461 ggctctcttg aatcaggaca atatgagaat caaaaagaag gtgcaggaag ctagccatgt
    20521 aggagataca gatacagccc tagaagaatt ggaacgttta atttcccaaa agagaagaat
    20581 ggagtaataa tggcaacaaa acaaaaagaa gtaacaacat ttgacgtaca ggtagcagaa
    20641 tttatccgta atcataagca aaaagggaca gcaacagatg atgaaatcaa tgcaagtctg
    20701 gttattcctt ttaccttgga cgctgatggg attgaagatc tcttgcaacg gattcaggat
    20761 gcaggcattt ctatcacaga taacgaagga aatccaagtg cgcgtgttct tagcaatgaa
    20821 gaagaaccag aactcagcga tgaggacttg attgggtcaa cttctgctaa ggtcaatgac
    20881 cctgtccgta tgtacttgaa agaaataggg gtcgttcctc tcttgaccaa tgaagaggag
    20941 aaagagttgg cactggctgt tgaagctggc gatatcgaag ccaaacaacg tcttgcggaa
    21001 gccaatcttc gtttggttgt ttccattgcc aaacgctatg tcggtcgtgg catgcagttc
    21061 cttgacttga ttcaagaagg aaatatgggc ttgatgaagg cggttgacaa gtttgactat
    21121 tctaaagggt tcaagttttc aacttatgca acttggtgga ttcgtcaggc tatcactcgt
    21181 gctattgcgg atcaagctcg taccatccgt atcccagttc acatggttga aactatcaat
    21241 aaattggttc gtgaacagcg gaatctcctt caagaattgg ggcaagatcc gacaccagaa
    21301 cagattgctg aacgaatgga tatgacacct gataaggttc gtgaaatctt gaagattgcc
    21361 caagaaccag tatctcttga aactcctatc ggtgaagagg acgatagcca ccttggagac
    21421 tttatcgaag atgaagtgat tgaaaatcca gtggattata cgactcgtat cgtcttgcgt
    21481 gagcaattgg atgaaatctt agatactctt acagaccgtg aagaaaatgt tctgcgtcta
    21541 cgttttggac tagatgatgg aaaaatgcgc acacttgaag atgtggggaa agtctttaac
    21601 gtaactcgtg agcgtatccg tcagattgaa gcaaaggctt tgagaaaact acgccaacca
    21661 agccgcagca aaccgcttcg tgattttatt gaagactaag agtgaggaaa atatggctta
    21721 tacagaagag caaattgaaa acatcaaaac acggatttta acagccttgg aagaagtcat
    21781 cgaccctgag ttgggaatcg atattgtcaa tcttggtttg atctatgaga ttcgttttga
    21841 cggtgacaca gggcaaacag agattgacat gactttgaca actatgggtt gtcccttagc
    21901 agaccttttg acagaccaga tttatgatgc tatgatagag gttccagaag taacggatac
    21961 tgaggttaaa ttagtctggt atccagcctg gacagttgaa aaaatgagtc gctatgcccg
    22021 cattgcccta ggcattaagt aaacatataa aagtatgtga gagactgttg gaaaagcgag
    22081 gaaatttccc ctcttttcct ctagtctctc ctttcttttg ctgattttat tcaaagaaaa
    22141 tgatataata gtagttatgg agaaaaagaa attacgcatc aatatgttga gttcaagtga
    22201 gaaagtagca ggacagggag tttcaggtgc ttaccgtgaa ttagttcgtc ttcttcaccg
    22261 tgctgccaag gaccaattga ttgttacaga aaatcttcca atcgaggcag atgtgactca
    22321 ctttcatacg attgattttc cctattattt atcaaccttc caaaagaaac gctcagggag
    22381 aaagattggc tatgtgcatt tcttgccagc tacacttgaa ggaagtttga aaattccatt
    22441 tttcttaaag ggaattgtga aacgctatgt attttctttt tacaaccgga tggagcactt
    22501 ggttgtggtc aatcctatgt ttattgagga tttggtagca gctggtattc cacgtgaaaa
    22561 agtgacctat attcctaact ttgtcaacaa ggaaaaatgg catcctctac cacaagaaga
    22621 ggtagtcaga ctgcgcacag atcttggtct tagtgacaat cagtttatcg tagtaggtgc
    22681 tgggcaagtt cagaaacgta aagggattga tgactttatc cgtctggctg aggaattgcc
    22741 tcagattacc tttatctggg ctggtggctt ctcttttggt ggtatgacag atggttatga
    22801 acactataag aaaattatgg aaaatccccc taaaaatttg atttttccag gcattgtatc
    22861 gccagagcgg atgcgcgaat tgtatgctct agcggatctt ttcttgttgc ctagttacaa
    22921 tgagctcttt cctatgacta ttttagaagc tgcgagttgt gaggctccta ttatgttgcg
    22981 tgatttagat ctctataagg tgattttgga gggaaattat cgggcgacag cgggtagaga
    23041 agagatgaaa gaggctattt tggaatatca agcaaatcct gctgtcttaa aagatctcaa
    23101 agaaaaggct aagaatattt ccagagagta ttctgaagag catctgttac aaatctggtt
    23161 ggacttttat gagaaacaag ccgctttagg gagaaagtaa aaagtgaggt aatctatgcg
    23221 aattggttta tttacagata cctattttcc tcaggtttct ggtgttgcga ccagtattcg
    23281 aaccttgaaa acagaacttg aaaagcaggg acatgctgtt tttatcttta cgacgacaga
    23341 taaggatgtc aatcgctacg aagattggca aattatccgc attccaagtg ttcctttctt
    23401 tgcttttaag gatcgtcgct ttgcctaccg aggttttagc aaggcacttg aaattgctaa
    23461 acagtatcag ctagatatta tccatactca gacagaattt tctcttggcc tgttggggat
    23521 ttggattgcg cgtgaattga aaattccagt catccatacc tatcacaccc agtatgaaga
    23581 ctatgtccat tatattgcta aggggatatt gatccggccg agtatggtca agtatctggt
    23641 tagaggtttc ctgcatgatg tggatggggt tatttgccct agtgagattg tccgtgactt
    23701 gctatctgat tataaggtca aggttgaaaa acgggtcatt cctactggga ttgaattagc
    23761 caagtttgag cgtccggaaa tcaagcagga aaatttgaaa gaactgcgta gtaaactagg
    23821 gattcaagat ggtgaaaaga cgttgcttag tctttcgaga atctcctatg aaaaaaatat
    23881 tcaagcagtt ttagcagcct ttgctgatgt tctgaaagag gaagacaagg ttaaactggt
    23941 agtagctggg gatggccctt atctgaatga cctcaaagag caagcccaga acctagagat
    24001 tcaagactca gtcatcttta cagggatgat tgctcctagt gagacggctc tttactataa
    24061 agcggcggat ttcttcattt cggcatcgac aagcgaaacg caaggtttga cctacttgga
    24121 aagcttagcc agtggaacac ctgtcattgc tcacggaaat ccttatttga acaacctcat
    24181 cagtgataaa atgtttggag ccttgtacta tggagaacat gatttggctg gtgctatttt
    24241 ggaagccctg attgcaacac cagacatgaa tgagcatacc ttatcagaga aattgtatga
    24301 gatttcagct gagaactttg ggaaacgagt gcatgagttt tatctggatg ccattatttc
    24361 aaataacttc cagaaagatt tggctaaaga tgatacggtc agtcagcgta tctttaggac
    24421 agttttgtat cttccgcaac aggtggttgc tgtacctgta aaaggatcta gacgcatgtt
    24481 gaaggcttca aaaacacagt tgatcagtat gagagactat tggaaagacc atgaagaata
    24541 gaaagaggaa cagctatgaa aaaaacaatt aatgagaagc ggtcgtgata aaaagattgc
    24601 gggtgtttgt gctggggtgg cccattatct ggatatggat ccgactatcg ttcaagtcat
    24661 ttggggtgtt cttacttgct gttacggagc tggaattgta gcttacatta ttttatggat
    24721 tatcgcacca gtagcaactg actattgaaa actagctcaa ttcatgctaa gataatagaa
    24781 aatagaatgt aacgaaggag agaaaaatgg catttggaga taatggaaat cgtaaaaaaa
    24841 ctatgtttga gaaaataacc ttgtttatcg tgattatcat gctagtagca agtttattgg
    24901 gaatttttgc aactgcaatt ggtgccctca gtaatctata aaatagattc aagaaaattt
    24961 agtgactggg atttcccagc ccttttttaa agtgagaaga aataatgagt atgtttttag
    25021 atacagctaa gattaaggtc aaggctggta atggtggcga tggtatggtt gcctttcgtc
    25081 gtgaaaaata tgtccctaat ggaggccctt ggggtggtga tggtggtcgt ggaggcaatg
    25141 tggtcttcgt tgtagacgaa ggactacgta ccttgatgga tttccgctac aatcgtcatt
    25201 tcaaggctga ttctggtgaa aaagggatga ccaaagggat gcatggtcgt ggtgctgagg
    25261 accttagagt tcgagtacca caaggtacga ctgttcgtga tgcggagact ggcaaggttt
    25321 taacagattt gattgaacat gggcaagaat ttatcgttgc ccacggtggt cgtggtggac
    25381 gtggaaatat tcgtttcgcg acaccaaaaa atcctgcacc ggaaatctct gaaaatggag
    25441 aaccaggtca ggaacgtgag ttacaattgg aactaaaaat cttggcagat gtcggtttag
    25501 taggattccc atctgtaggg aagtcaacac ttttaagtgt tattacctca gctaagccta
    25561 aaattggtgc ctaccacttt accactattg taccaaattt aggtatggtt cgcacccaat
    25621 caggtgaatc ctttgcagta gccgacttgc caggtttgat tgaaggggct agtcaaggtg
    25681 ttggtttggg aactcagttt ctccgtcaca tcgagcgtac acgtgttatc cttcacatca
    25741 ttgatatgtc agctagcgaa ggccgtgatc catatgagga ttacctagct atcaataaag
    25801 agctggagtc ttacaatctt cgcctcatgg agcgtccaca gattattgta gctaataaga
    25861 tggacatgcc tgagagtcag gaaaatcttg aagaatttaa gaaaaaattg gctgaaaatt
    25921 atgatgaatt tgaagagtta ccagctatct tcccaatttc tggattgacc aagcaaggtc
    25981 tggcaacact tttagatgct acagctgaat tgttagacaa gacaccagaa tttttgctct
    26041 acgacgagtc cgatatggaa gaagaagctt actatggatt tgacgaagaa gaaaaagcct
    26101 ttgaaattag tcgtgatgac gatgcgacat gggtactttc tggtgaaaaa ctcatgaaac
    26161 tctttaatat gaccaacttt gatcgtgatg aatctgtcat gaaatttgcc cgtcagcttc
    26221 gtggtatggg ggttgatgaa gcccttcgtg cgcgtggagc taaagatggg gatttggtcc
    26281 gcattggtaa atttgagttt gaatttgtag actaggagac tggtatggga gataaaccga
    26341 tatctttccg agatgcggat ggtaattttg tttccgccgc agacgtttgg aatgaaaaga
    26401 aattggaaga actatttaat cgtctcaatc caaatcgtgc cttgagattg gcacgaacta
    26461 aaaaggaaaa tccatctcag taaagaagct aaaaaatccc gtgcctcatc agacacggga
    26521 ttttgtggta cgacaggcat gtatagcaaa ctgaatctgg aatagcacag catatcttct
    26581 aaaatatagt aaaatgaaat gagaacagga caaatcgatc aggacagtaa aatcgatttc
    26641 taacaatgtt ttagaagcag agatgtacta ttctagtttc aatcaactat attgttataa
    26701 attgatttga atttcaaaat taaattgttt gattcttatt tcaatttgtt atagtatatc
    26761 tgatgtcaaa gttctcggcg agtcaaatag cgattcccaa gcctgactat cgtgaggtag
    26821 cggattaaaa tggtctgggg atagaccgtt ttaagtctga cgctggaaat aagaattgtc
    26881 agaagaaggg atagcgaaat cgtggctcta cgaacaggga cgtgataata aggcgtatat
    26941 agcggataag agggcatcaa actctaaagt ccaaaaaggt agtcgtaacc tatatgcgta
    27001 aatcacgaga gtaattgaat tcgtactaag attttctatt ttcactgtaa ccttttaacg
    27061 cccttatatc ttgtatacac gaggaaagat gtacgactta tcccgtgagg tctatcacta
    27121 taaagagaaa acgacagata gaagtgatcc tgagtcacgg ttatctgtct gataggacgg
    27181 tatgtataaa acgcttctgt gaactgagag aagggggaga agttcttgct aaaatttagt
    27241 tgaacagccg tattccgata cttagataag agatctagtc ttagctccta ctcagtttta
    27301 ggggataaaa aaggggcaat agcgattcga gaaagattat actcttcgaa aatctcttca
    27361 aatcacgtca atatcgcctt gtcgtatgtg taggatactg actacgtcag ttccatctac
    27421 aacctcaaaa cagtgttttg agcaacctgc ggctagtttc ctagtttgat ctttgatttt
    27481 cattgagtat tagtaattca gttactaact cgtcaactct gatttatcca ataaaattga
    27541 aaaggatgga aaaaaggata aatttatgat atactttatt ttgaagacct tattagaaat
    27601 cttgaaagag tattgaaaac ttagaatgag aaaaattgtt atcaatggtg gattaccact
    27661 gcaaggtgaa atcactatta gtggtgctaa aaatagtgtc gttgccttaa ttccagctat
    27721 tatcttggct gatgatgtgg tgactttgga ttgcgttcca gatatttcgg atgtagccag
    27781 tcttgtcgaa atcatggaat tgatgggagc tactgttaag cgttatgacg atgtattgga
    27841 gattgaccca agaggtgttc aaaatattcc aatgccttat ggtaaaatta acagtcttcg
    27901 tgcatcttac tatttttatg ggagcctctt aggccgtttt ggtgaagcga cagttggtct
    27961 accgggagga tgtgatcttg gtcctcgtcc gattgactta caccttaagg cgtttgaagc
    28021 tatgggtgcc actgctagct acgagggaga taacatgaag ttatctgcta aagatacagg
    28081 acttcatggt gcaagtattt acatggatac ggttagtgtg ggagcaacga ttaatacgat
    28141 gattgctgcg gttaaagcaa atggtcgtac tattattgaa aatgcagccc gtgaacctga
    28201 gattattgat gtagctactc tcttgaataa tatgggtgcc catatccgtg gggcaggaac
    28261 taatatcatc attattgatg gtgttgaaag attacatggg acacgtcatc aggtgattcc
    28321 agaccgcatt gaagctggaa catatatatc tttagctgct gcagttggta aaggaattcg
    28381 tataaataat gttctttacg aacacctgga agggtttatt gctaagttgg aagaaatggg
    28441 agtgagaatg actgtatctg aagacagcat ttttgtcgag gaacagtcta atttgaaagc
    28501 aatcaatatt aagacagctc cttacccagg ctttgcaact gatttgcaac aaccgcttac
    28561 ccctctttta ctaagagcga atggtcgtgg tacaattgtc gatacgattt acgaaaaacg
    28621 tgtaaatcat gtttttgaac tagcaaagat ggatgcggat atttcgacaa caaatggtca
    28681 tattttgtac acgggtggac gtgatttacg tggggccagt gttaaagcga ccgacttaag
    28741 agctggggct gcactagtca ttgctgggct tatggctgaa ggtaaaactg aaattaccaa
    28801 tatcgagttt atcttacgtg gttattctga tattatcgaa aaattacgta atttaggagc
    28861 ggatattaga cttgttgagg attaaaccgt agaggtgttt atgaatattt ggaccaaatt
    28921 agcaatgttt tctttttttg aaacggatcg cttgtatttg cgtcctttct tttttagtga
    28981 tagtcaggac ttccgcgaga tagcttcaaa tccagaaaat cttcaattta ttttcccaac
    29041 gcaggcaagt ctggaagaaa gtcaatatgc actggccaat tactttatga agtccccttt
    29101 gggagtgtgg gcaatttgtg accagaaaaa tcaacaaatg attggttcta ttaaatttga
    29161 gaagttagat gaaatcaaaa aagaagctga gcttggctat tttttgagaa aagatgcttg
    29221 gtcgcaagga tttatgacag aggttgttag aaaaatttgt cagctttctt ttgaggaatt
    29281 tggcttaaaa caattattta tcattaccca ccttgaaaat aaagctagcc aaagagttgc
    29341 tcttaagtct ggatttagtt tgttccgtca gtttaaggga agtgatcgtt acacaagaaa
    29401 aatgcgggat tatcttgaat ttcggtatgt aaaaggagag ttcaatgagt aagcatcagg
    29461 aaattctaag ctatttggag gaattaccag taggtaaaag ggtcagtgtt cgtagcattt
    29521 cgaatcatct aggagttagt gatggaacag cctatcgggc tattaaagaa gctgaaaacc
    29581 gtggaattgt ggagacccgt cctagaagtg gaacaattcg tgttaaatcc cagaaagttg
    29641 ctatagagag attaacgttt gctgaaattg cagaagtgac ttcttctgag gttctggctg
    29701 ggcaagaagg tttagagaga gaatttagta agttttcaat tggtgccatg actgaacaaa
    29761 atatcttgtc ttaccttcat gatggggggc tcttgattgt cggagaccga acccgtattc
    29821 agttgctagc cttggaaaat gaaaatgcag ttctggttac agggggattt caggttcatg
    29881 atgatgtgct taaactggcc aatcaaaaag ggattcctgt tctaagaagt aagcatgata
    29941 cctttaccgt cgcgaccatg atcaataaag ccttgtcaaa tgtccaaatc aagactgata
    30001 ttctgacagt tgagaaactt tatcgcccta gtcatgagta tggttttctg agagagacag
    30061 atacagttaa agattatttg gacttggttc gtaagaatcg tagcagccgt ttccctgtta
    30121 tcaatcaaca tcaggtcgtt gttggcgttg taaccatgag agacgctggt gataaatcac
    30181 caagcacgac aattgataag gttatgtctc gtagtctatt tttggttgga ttatcgacaa
    30241 atattgccaa tgtgagtcaa cggatgatcg cagaagactt tgaaatggta ccagttgttc
    30301 gaagcaatca aactttgctt ggcgttgtga cgcgacgaga tgtcatggag aagatgagcc
    30361 gttctcaagt ttcggctcta ccaacttttt ctgagcagat tggacaaaag ctctcttatc
    30421 accatgatga agtagtcatt acagtggaac cctttatgct agaaaaaaat ggagttttgg
    30481 ctaatggtgt attggcagaa attctgaccc acatgaccca agatttagtt gttaatagtg
    30541 gtcgcaatct cattatcgag cagatgctaa tctacttttt gcaggctgtt cagatagatg
    30601 atatattgcg cattcaggca cggattattc atcatacgag acggtcagct ataattgatt
    30661 acgatattta tcatggtcac cagattgttt caaaagcaaa tgtgactgtt aaaattaatt
    30721 agaaactagg agaaaagatg ataacattaa aatcagctcg tgaaatcgaa gctatggaca
    30781 aggctggtga ttttctagca agtattcata taggcttacg tgatttgatt aagccaggcg
    30841 tagatatgtg ggaagttgaa gaatatgtcc gccgtcgttg taaagaagaa aatttccttc
    30901 cacttcagat tggggttgac ggtgccatga tggactatcc ttatgctacc tgttgctctc
    30961 ttaacgatga agtggctcac gctttccctc gtcattatat cttgaaagat ggtgatttgc
    31021 tcaaagttga tatggttttg ggaggtccca ttgctaaatc cgacctgaat gtctcaaaat
    31081 taaacttcaa caatgttgaa caaatgaaaa aatacactca gagctattct ggtggtttag
    31141 cagactcatg ttgggcttat gctgttggta caccgtccga agaagtcaaa aacttgatgg
    31201 atgtaaccaa agaagctatg tacaagggta ttgagcaagc tgttgttgga aatcgtatcg
    31261 gtgatatcgg tgcggctatt caagaatacg ctgaaagtcg tggttacggt gtagtgcgtg
    31321 atttggttgg tcatggtgtt ggcccaacta tgcacgaaga accaatggtt cctaactatg
    31381 gtattgcagg tcgtggactc cgtcttcgtg aaggaatggt cttaaccatt gaaccaatga
    31441 tcaatacagg cgattgggaa attgatacag atatgaaaac tggttgggcg cataagacca
    31501 ttgacggtgg attgtcatgt cagtatgaac accaatttgt cattacgaaa gatggacctg
    31561 ttatcttgac tagccaaggt gaagaaggaa cttattaata aaaagtgaaa agactactgg
    31621 aagtttattt tgataaaaaa tccagtagat cttttcataa taaaacgcat tgtatcaagt
    31681 gttaggggct gatatcatgc gtttttctgc ttttaagatt ttttccaact ctgtttgtaa
    31741 gcgcatcata acaaagggtc taggattcag ggctctcctc ctatatacta ttagtaaagt
    31801 aaaactaagg gaggatattt tagtgtcgca gtctattgtt cctgtagaga ttccacaata
    31861 ttgtcgtttt gattctaaaa agagaaatgg aattctgttt aatgttcgta ttgccaatct
    31921 taaatttacc ttttttagat tagacttcct gcgaaataaa atatggtata gtagttctat
    31981 gaatgatgaa gcaagtaaac aactaaccga ttcacgattt aag
//
LOCUS       DBERMR010000024        31234 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.024, whole genome shotgun sequence.
ACCESSION   DBERMR010000024 DBERMR010000000
VERSION     DBERMR010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 31234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31234
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.024"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            484..1527
                     /locus_tag="ACL8A1_001166"
     CDS             484..1527
                     /locus_tag="ACL8A1_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HGS1201946.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGMGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            1694..3781
                     /gene="topA"
                     /locus_tag="ACL8A1_001167"
     CDS             1694..3781
                     /gene="topA"
                     /locus_tag="ACL8A1_001167"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HGS1201947.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            3887..4246
                     /locus_tag="ACL8A1_001168"
     CDS             3887..4246
                     /locus_tag="ACL8A1_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HGS1201948.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            4339..4971
                     /locus_tag="ACL8A1_001169"
     CDS             4339..4971
                     /locus_tag="ACL8A1_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HGS1201949.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDICLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLDNRQTFIDQVGVTQLHGTKVVF"
     gene            5015..5671
                     /locus_tag="ACL8A1_001170"
     CDS             5015..5671
                     /locus_tag="ACL8A1_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HGS1201950.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            5741..6784
                     /locus_tag="ACL8A1_001171"
                     /pseudo
     CDS             5741..6784
                     /locus_tag="ACL8A1_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261078.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
     gene            6796..7833
                     /gene="leuB"
                     /locus_tag="ACL8A1_001172"
     CDS             6796..7833
                     /gene="leuB"
                     /locus_tag="ACL8A1_001172"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HGS1201951.1"
                     /translation="MAKKIVALVGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAGPPLPDETLKASREADAILLVAIGSPQYDGVAVRPEQGLMALRKELNLYAN
                     IRPVKIFDSLKYLSPLKPERISGVDFVVVRELTGEIYFGDHILEERKARDINDYSYEE
                     VERIIRKAFEIARNRRKIVTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSA
                     AMLMITNPAKFDVIVTENLFGDILSDESSVLSGTLGVMPSASHSENGPSLYEPIHGSA
                     PDIAGQGIANPISMILSVVMMLRDSFGRYEDTERIKRAVETSLAAGILTRDIGGQAST
                     KEMMEAIIARL"
     gene            7830..8099
                     /locus_tag="ACL8A1_001173"
     CDS             7830..8099
                     /locus_tag="ACL8A1_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HGS1201952.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRAWRIPEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            8103..8462
                     /gene="leuD"
                     /locus_tag="ACL8A1_001174"
     CDS             8103..8462
                     /gene="leuD"
                     /locus_tag="ACL8A1_001174"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HGS1201953.1"
                     /translation="MAGSSREYAAWALADYGFKVVIAGSFGDIHYNNELNNGMLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            8624..9194
                     /locus_tag="ACL8A1_001175"
                     /pseudo
     CDS             8624..9194
                     /locus_tag="ACL8A1_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004915491.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
     gene            9288..9353
                     /locus_tag="ACL8A1_001176"
                     /pseudo
     CDS             9288..9353
                     /locus_tag="ACL8A1_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849983.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
     gene            9516..11429
                     /locus_tag="ACL8A1_001177"
     CDS             9516..11429
                     /locus_tag="ACL8A1_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HGS1201954.1"
                     /translation="MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEW
                     PLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNK
                     YKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
                     LLCIYSGGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKL
                     GTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEI
                     AKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKE
                     TQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLP
                     SKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEI
                     NRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDR
                     SVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENVQELNSH
                     YHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNDQ
                     TGQQDTGDNDADY"
     gene            11416..12732
                     /locus_tag="ACL8A1_001178"
     CDS             11416..12732
                     /locus_tag="ACL8A1_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HGS1201955.1"
                     /translation="MRITDNQYKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKILHINLTSLDVALSREERLYQLLVYLFPKYLQAAIRKGLYKEYHRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYVTREFTYDNPLMQLVRYTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLIFPSMEQSVNSEIGKV
                     AGYGAQLKKWSIRIPQNASFYSTFCKMMENSEENFKAIIDEEVGRK"
     gene            12907..13893
                     /locus_tag="ACL8A1_001179"
     CDS             12907..13893
                     /locus_tag="ACL8A1_001179"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HGS1201956.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHDQGLIASISVGVKDYEY
                     DFVRQLKTDAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            13957..14028
                     /locus_tag="ACL8A1_001180"
     tRNA            13957..14028
                     /locus_tag="ACL8A1_001180"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13990..13992,aa:Arg,seq:ccg)
     gene            14211..14909
                     /gene="rnc"
                     /locus_tag="ACL8A1_001181"
     CDS             14211..14909
                     /gene="rnc"
                     /locus_tag="ACL8A1_001181"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HGS1201957.1"
                     /translation="MKELQTVLKNHFAIEFTDKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            14900..18439
                     /gene="smc"
                     /locus_tag="ACL8A1_001182"
     CDS             14900..18439
                     /gene="smc"
                     /locus_tag="ACL8A1_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HGS1201958.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLATLEDKRNS
                     LSKEKYDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDNLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDMHYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDAIAVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAEEEASLGSEEATLKTLQDQMARLSELLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEELDRLSDGDWQADKEKCQDRLATIASDKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLNIEQEEIQRMLQEKVDN
                     LEKVDTELLSQQAEESKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQNQLTDQYQISYTEALEKAHELENLNLAEQEVQDLEK
                     AIRSLGPVNLEAIDQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            18436..19230
                     /locus_tag="ACL8A1_001183"
     CDS             18436..19230
                     /locus_tag="ACL8A1_001183"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGS1201959.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNEHVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKDQSVIAYMEGL"
     gene            19230..20048
                     /locus_tag="ACL8A1_001184"
     CDS             19230..20048
                     /locus_tag="ACL8A1_001184"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGS1201960.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSNQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAVANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            20052..21341
                     /gene="ftsY"
                     /locus_tag="ACL8A1_001185"
     CDS             20052..21341
                     /gene="ftsY"
                     /locus_tag="ACL8A1_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HGS1201961.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDIEPAFTEVEEVS
                     QEEAEVEIVEQAVFQEEEIQDTVEESQDSEPAVGVSQEEIEELPNSEELPEEEKLEHE
                     GTIEENNSEILEAERPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEE
                     LEELLIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDES
                     IHFQDNLTVMLFVGVNGVGKTTSIGKLAHRYKQAGKKVMLVAADTFRAGAVAQLAEWG
                     RRVDVPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGR
                     IIKRVVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAI
                     REELNIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(21387..22874)
                     /gene="zwf"
                     /locus_tag="ACL8A1_001186"
     CDS             complement(21387..22874)
                     /gene="zwf"
                     /locus_tag="ACL8A1_001186"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HGS1201962.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVCASWKLIDRIEKLWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDITYRQDGRLE"
     gene            complement(23023..23763)
                     /locus_tag="ACL8A1_001187"
     CDS             complement(23023..23763)
                     /locus_tag="ACL8A1_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGS1201963.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(23763..25928)
                     /locus_tag="ACL8A1_001188"
     CDS             complement(23763..25928)
                     /locus_tag="ACL8A1_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HGS1201964.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALSEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRIISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            26100..27053
                     /locus_tag="ACL8A1_001189"
     CDS             26100..27053
                     /locus_tag="ACL8A1_001189"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HGS1201965.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAYTLYGIG
                     LLALIGLARNFGEAGQNLATYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNFGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            27130..29118
                     /gene="uvrB"
                     /locus_tag="ACL8A1_001190"
     CDS             27130..29118
                     /gene="uvrB"
                     /locus_tag="ACL8A1_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HGS1201966.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAVAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            29128..29691
                     /locus_tag="ACL8A1_001191"
     CDS             29128..29691
                     /locus_tag="ACL8A1_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HGS1201967.1"
                     /translation="MIYLRKLKKEDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKETRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLVLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            29657..30145
                     /locus_tag="ACL8A1_001192"
     CDS             29657..30145
                     /locus_tag="ACL8A1_001192"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1201968.1"
                     /translation="MTVIIKSMETPEEIEGKSLVHWQTWRESYDDLLPAEFQDTMTLE
                     RCRFFSQKYPENTLIAMDGVKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SK"
     gene            30219..30683
                     /locus_tag="ACL8A1_001193"
     CDS             30219..30683
                     /locus_tag="ACL8A1_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HGS1201969.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGTLQS
                     SEEGEVSWVQKDQIPNLNLAYDMLPLMEMMEAPDKSEFFYPRRTDLTFPSLR"
BASE COUNT         9675 a         5502 c         6911 g         9146 t
ORIGIN      
        1 gtgcactgtt atagattcaa tacactataa tttagtgtaa ttgagaaagg agaaatgatt
       61 gtgattgatg ttggctaggt tatgttcaat gattcctacc gtctcaaatc ttgtcagtaa
      121 ggaaaaataa attcttcaaa agtagagatt acaaggcttg tttaagaaag aattcaaaga
      181 ccttgacaaa taaaaataaa atggttatta taaaaaatgg tctgaaatag atgatgatac
      241 ttttcgaaaa tctttttacg tcagctcagc tttgccttgc tgtgttttga gcaagctacg
      301 gttagcttcc gagtttgatt ttcatttact agaaatgaaa ctgatgagag atatcagtag
      361 acatctgagt caggatatta tggaaaatga taaaaagagc tcgtgagatt ggcatatcag
      421 actactaaag tattgagttt gttaggattt tagcgactag ttggctggga aaggaagata
      481 tttgtgacaa ataataaact gtattcgttg atagaattta gaaataaaat atatgaagaa
      541 ttagaacttt ccagaagtga tttagcgatt ttactatgtg ccatgcttat cgcctctatc
      601 ggattaaata tggattcgac tcccgtgatt attggagcca tgttaatctc tcctttgatg
      661 acacctattc tgggaatggg gctctctcta gctatatttg attttaaatt gttaagaaaa
      721 tcttttaaaa tattagctat tcaaattctt gccagtctaa tagcttcaac actttatttt
      781 tatctttctc ccatttcgta tgctagttcg gagattgttg ctagaacctc tccgactatt
      841 tgggatgttc tcattgcttt tgtaggaggg atagcaggta ttattggtgc taggaaaaaa
      901 gagaccaata atattgttcc tggtgttgct attgcaaccg ccttgatgcc tcctctttgt
      961 acagtaggtt atgctattgc ttctgctaat ctaaaattta tcataggctc ctcttaccta
     1021 ttcctcatca attgtagctt tattgtcatt gcgacttata taggtgttag gttgatgatg
     1081 gttaagaaac attattttaa agataatgaa gaagactcta aaatgcgtag gattttgctt
     1141 ctagttgctg ttttgctgat gattccgagt ttcatctctg caacgacttt agtgagagaa
     1201 acgttgaaaa aagagtccct taagaaattt atatcagagc agtttcaggg gtataatatt
     1261 ttgaaaaaaa cctattctaa aaagactcat accctaaagc taaccatttc aggaaattat
     1321 ttgacagaag aagaactcga tatgatttca agtaagagag gtgactatgg tttaagtgat
     1381 gtttctgttc aagtttcaca attgtctgat tcagaacaac ttagtaagga agagctggtg
     1441 gagtatttct tccagtatat caaggataag gaagcaaaag aaaaggagaa agccaataaa
     1501 tttgatacag agtctgagga gcaataattt cttgagaata gctggtttct ctcgtgagtc
     1561 ttctatgtat atcaaaggaa gactgaggtt ttaagtataa aacttttctt ctattatagt
     1621 agctaagtct tgacagggtt caaaaaatgg tttacacttt ataaagttta ttactttgaa
     1681 aaggtgtgat actgtggcta cggcaacaaa aaagaaaaaa tcaacagtta aaaaaaatct
     1741 agtcatcgtg gagtcgcctg ctaaggccaa gacgattgaa aaatatctag gcagaaacta
     1801 caaggtttta gccagtgtcg ggcatatccg tgatttgaag aaatccagta tgtccgtcga
     1861 tattgaaaat aattatgaac cgcaatatat taatatccga ggaaaaggcc ctcttatcaa
     1921 tgacttgaaa aaagaagcta aaaaagctaa taaagttttt ctcgcgagtg acccggaccg
     1981 tgaaggagaa gcgatttctt ggcatttggc ccatattctc aacttggatg aaaatgatgc
     2041 caaccgtgtg gtcttcaatg aaatcaccaa ggatgcagtc aaaaatgctt ttaaagaacc
     2101 tcgtaagatc gatatggact tggtcgatgc ccaacaagct cgtcggatct tggatcgctt
     2161 ggtagggtat tcgatttcgc ctattttgtg gaagaaggtc aagaagggct tgtcagcagg
     2221 tcgcgttcag tccattgccc ttaaactcat cattgaccgt gaaaatgaaa tcaatgcctt
     2281 ccagccagaa gaatactgga cagttgatgc tgtctttaaa aagggaacca aacaatttca
     2341 tgcttccttc tatggagtag atggtaaaaa gatgaaactg accagcaatg acgaagtcaa
     2401 ggaagtcttg tctcgtctga ctagtaaaga cttttcagta gatcaggtgg ataagaaaga
     2461 gcgcaagcgc aatgctcctt taccctatac cacttcatct atgcagatgg atgctgccaa
     2521 taaaatcaat ttccgtactc gaaaaaccat gatggttgcc caacagctct atgaaggaat
     2581 taatatcggt tctggtgttc aaggtttgat tacctatatg cgtaccgatt cgactcgtat
     2641 cagtcctgta gcgcaaaatg aggcggcaag cttcattacg gatcgttttg gtagtaagta
     2701 ttctaagcac ggtagcaagg tcaaaaacgc atcaggtgct caggatgccc atgaggctat
     2761 tcgtccgtca agtgtcttta atacaccaga aagcatcgct aagtatctgg acaaggatca
     2821 gctcaagcta tataccctta tctggaatcg ttttgtggct agccagatga cagcggccgt
     2881 ttttgatacc atggctgtta aattgtctca aaaaggggtt caatttgctg ccaatggtag
     2941 tcaggttaag tttgatggtt atcttgccat ttataatgat tctgacaaga ataagatgtt
     3001 accggacatg gttgttggag atgtggtcaa acaggtcaat agcaaaccag agcaacattt
     3061 cacccaaccg cctgcccgtt attctgaagc aacactgatt aaaaccttag aggaaaatgg
     3121 ggttggacgt ccatcaacct acgcgccaac cattgaaacc attcagaaac gttattatgt
     3181 tcgcctggca gccaaacgtt ttgaaccgac agagttggga gaaattgtca ataagctcat
     3241 cgttgaatat ttcccagata tcgtaaacgt gaccttcaca gctgaaatgg aaggtaaact
     3301 ggatgatgtc gaagttggaa aagagcagtg gcgacgggtc attgatgcct tttacaaacc
     3361 attctctaaa gaagttgcca aggctgaaga agaaatggaa aaaatccaga ttaaggatga
     3421 accagctgga tttgactgtg aagtgtgtgg cagtccaatg gtcattaaac ttggtcgttt
     3481 tggtaaattc tacgcttgta gcaatttccc agattgccgt catacccaag caatcgtgaa
     3541 agagattggt gttgagtgtc caagctgtca tcagggacaa attattgagc gaaaaaccaa
     3601 gcgtaatcgc ctcttctatg gttgcaatcg ctatccagaa tgtgaattta cctcttggga
     3661 caagcctgtt ggtcgtgact gtccaaaatg tggcaacttc ctcatggaga aaaaagtccg
     3721 tggtggtggc aagcaggttg tttgtagcaa aggcgattac gaggaagaaa agattaaata
     3781 agaggagggg cccgaaagta aatttcaggc tccttttggt tagaacttga caaaatacat
     3841 cattttatgc gaaactagaa gaagattatt ttaactagga gaagttatgc gtcttatcta
     3901 tctgataatt ggttttttat cactggcctt ggctattgtt ggggttgttt tacccttgtt
     3961 gccgacaacg cctttccttt tgttgtctat tgcttgtttc tccagaagtt ccaagcgttt
     4021 cgaagactgg ctttatcata ccaagctcta tcaaacatat gtagctgatt ttcgcgagac
     4081 caagtctatt acgcgtgaac gaaagaaaaa aatcatcgtc tctatctacg tcttgatggg
     4141 aatttctatt tattttgcac ctcttttacc agtcaaaatc ggtttgggtg ctttgaccat
     4201 ctttatcact tattatctct tcaaggtcat tccagacaaa gaatagttaa aacagtagtt
     4261 atttaccttg ataaaattga aagcatattc ataacaatat gatataataa atttgaagta
     4321 atattcaagg agaatcaaat gatttacgaa ttttgtgctg aaaatgtgac tttacttgaa
     4381 aaagcgatgc aggctggagc tcgtcggatt gaactctgtg ataatctagc agttggtggg
     4441 acaacaccta gctatggagt gactaaggca gcggttgaac tggcagctaa ctacgataca
     4501 accatcatga ccatgattcg gccacgtggt ggtgactttg tctataatga cctagaaatt
     4561 gctatcatgc tagaagacat ttgtttgact gctcaggctg gaagtcaagg ggttgtattt
     4621 ggagctttaa ctgctgataa aaagttggat aagcctaatc tggaaaagtt aattgctgca
     4681 tcaaaaggaa tggaaattgt ctttcacatg gcctttgatg aactaagtga tgaagatcaa
     4741 ccggaagcta ttgactggct cagtcaagcc ggtgtcactc gtatcctaac tcgtgctggt
     4801 gtgtctggcg actccttaga aaaacgtttt gttcactatc acagaatttt ggagtacgct
     4861 aaaggtaaaa ttgaaattct accaggtggg gggattgacc ttgacaaccg tcaaaccttt
     4921 attgaccagg tgggggtaac acaattgcat ggtactaagg ttgtttttta aaaaatagaa
     4981 aggaactgct agccttgggt agcagttttc acttatgttt gaaattttta aatcctatca
     5041 atttaatcaa gaaaaggctc atgattatgg ttttatagaa aatagcgaag tctggacata
     5101 tagttgccag attttgcaag gtgactttgt catgactgtg tccatcactg ctgataatgt
     5161 gaactttcaa gtctttgacc aagagactgg tgacctctat cctcacgttc atatggaaag
     5221 catgagggga agttttgtcg gaaatgtccg tgaggcttgt ctggagattc tttaccagat
     5281 tcggaaggct tgttttgatg tgcaagattt tatctgtcat cagactaagc gtatcatgac
     5341 tcaagttcag gaaaagtatg gaaaccagtt ggagtatctg tgggaaaaat cgcctgatac
     5401 agctgtattg cgccatgaag gcaatcaaaa gtggtatgcc gtcttgatga aaatctcttg
     5461 gaataagctg gaaaagggca gagaaggaca agtggaagca gtcaacctca agcatgacca
     5521 agtagctaat ttgctttcac aaaaggggat ttatccagcc ttccatatga gcaagcgcta
     5581 ctggattagt gtgtcccttg atgatacttt atcagatgaa gaagtactgg aattgataga
     5641 aaaaagttgg aacttaacct ctaaaaaatg aaatatttta ataattttca tgaactttca
     5701 attagctaaa tattctttac tgaagaaatt tttagaaaat ataggattta ccacactaga
     5761 ggaatatggt gccatcttca aatacctgat tgagaatgtc aagacggatc gtcagatcat
     5821 ctgttcgcct cactgtcatg atgacctcgg aatggcagtg gcaaatagcc ttgctgctgt
     5881 caagaatggt gcaggacgtg ttgaagggac tatcaatggt attagggagc gagctgaaaa
     5941 tgctgctttg gaagaaattg cagtggctct caatattcgc caagattact accaagtaga
     6001 aaccagtatt gtcctaaatg agaccatcaa tacgtcagaa atggtttctc gcttctctgg
     6061 tattccagtt cctaaaaaca aagccgtcgt tggtggcaat accttctccc acgaatctgg
     6121 tattcaccaa gatggagtcc ttaaaaatcc tctcacttat gagatcatca cacctgaatt
     6181 ggttggtgtt aagattccgc ttggagaatt atctggtcgc catgcttttg ttgagaaact
     6241 gagagaattg gccctagatt ttacagaaga ggatatcaaa ccactctttg ctaagttcaa
     6301 ggcactggcc gataagaagt aagaaatcac agatgcagat actcgagctt tggtagctgg
     6361 aaccatggtt gaaaatccag aaggcttcca ctttgatgat ttacaacttc aaactcatgc
     6421 agataatgac attgaagcgc tcgttagcct agccaatatg gatggtgaga aagtcgaatt
     6481 taatgcgaca gggcaaggtt ccgttgaagc aatctttaac gctatcgata agttctttaa
     6541 ccaatctgtt cgtttggtgt cctacactat caatgcggta acagatggaa tcgatgccca
     6601 ggatcgggtt ttggtcactg ttgaaaacag agatacagaa accatcttta atgcagcagg
     6661 gcttgatttt gatgtgttga aggcttctgc tattgcctat ataaacgcta atacctttgt
     6721 tcaaaaagag aatgcaggtg agatgggacg cagtgtttct taccacgata tgcctagtgt
     6781 gtaaaggaga aggctatggc aaagaaaata gtagctctag taggagacgg aattggccca
     6841 gaaatcatgg aggctggttt agaagttctg gaggctctag ctgaaaaaac aggttttgac
     6901 tatgagattg acagacgacc gttcggaggt gcagatattg atgcagcagg acctccctta
     6961 cctgatgaaa cccttaaggc aagtagggaa gcagatgcta tcctactagt agctatcggt
     7021 agtcctcagt atgatggagt agcggttcgc cctgaacaag gcctgatggc tctccgtaag
     7081 gaactcaatc tttacgctaa tattcgtcct gtaaaaatct ttgacagtct caagtatttg
     7141 tcaccactca aaccggaacg aatttctggt gtagactttg tcgtggtgcg tgaattgaca
     7201 ggcgagattt actttggaga tcatattctt gaagagcgca aagcgcgtga tatcaacgac
     7261 tatagctatg aggaagtgga gcggattatt cgcaaagcct ttgaaattgc aagaaatcgc
     7321 agaaaaatcg ttactagtat cgataagcaa aatgttctag cgacctcaaa actctggcgg
     7381 aaagtagctg aggaagtcgc acaggatttc ccagatgtaa ccttggaaca ccagctggta
     7441 gactcagctg ctatgcttat gattaccaat cctgctaagt ttgatgttat tgtaacggag
     7501 aatctttttg gagatatttt atctgatgaa tcaagcgtct tatctggtac acttggggtt
     7561 atgccatcag ccagtcattc tgaaaatgga ccaagtctct atgaacctat tcacggttca
     7621 gcacctgata ttgcaggtca aggaattgcc aatcctattt ccatgatttt atcagttgtc
     7681 atgatgttga gagatagttt cggacgttat gaggatacag agcgtatcaa acgtgctgtt
     7741 gagacaagtc tggcggcagg aattttaacg agagatatag gaggtcaggc ttcaacaaag
     7801 gaaatgatgg aagctattat tgcaaggtta tgaagttaga cgaaaaaatt actctagtcc
     7861 ttttgatttg gaatgtcatc attttcttga tttatggcat tgacaaattc aaggcaagaa
     7921 gaagagcttg gcgcatccca gagaaaatct tactcatttt agcccttact tgtggtggtt
     7981 ttggggcctg gttagctgga atcacttttc accacaagac tcgaaaatgg tactttaaaa
     8041 cagtttggtt tcttgggatg gtgaccacac tagtagcctt atattttatt tggaggtaat
     8101 ggatggcagg gtcttcgagg gaatacgctg cttgggctct agcggactat ggttttaagg
     8161 tcgtgattgc aggatctttc ggtgacattc attacaataa tgaactcaat aatggcatgt
     8221 tgccaatcgt tcagcctaga gaggttagag agaaactagc ccagctaaaa ccaaccgacc
     8281 aggtaactgt ggacttggaa caacaaaaaa tcatctcacc agttgaagaa ttcaccttcg
     8341 agatagatag cgagtggaaa cataaactcc taaatagttt ggatgatatc ggtattacct
     8401 tgcagtatga agagttgatt gctgcttatg aaaaacaacg accagcctac tggcaggatt
     8461 agaaaaaata gaaaaggaaa tatagtaaac tgaaataaga tgtaaacaaa tgaattggag
     8521 cttaacatcc atttccagca attttttaga aactacagtg gactattctg gattcaacac
     8581 attataaaat tatgacaaaa cacattcaca agaaggctac gacattttaa aaggtgaggg
     8641 cggatgtatc gtttgcccta ctaaagttgg ttacattatc atgaccagtg acaaggcagg
     8701 acttgagcgt aagttcgcag ccaaagaacg taagcgtaac aaaccaggtg ttgttctctg
     8761 cggtagcatg gatgaacttc gcgctttagc gcaactcaac ccagaaattg aagcattcta
     8821 ctaaaaacat tgggatgaag atattcttct tggttgtatc cttccttgga aaccagaagc
     8881 ctctgaaaaa ctcaaagcat acggggatgg ccgtgaagaa cttatgactg atgtacgtgg
     8941 tactagctgt tttgttatca agtttggaaa agcaggtgaa caattggctg ccaagctttg
     9001 ggaagaaggt aaaatggtct acgcctcatc tgcttcaatg acaaaacgat tgaaactcgc
     9061 tatgagagca aggtgtaatg gtgtctatgg tcgataagga cggcaaactc atcccagaac
     9121 aaggaggagc acgttcaact tcaccagcac cagttgtgat ccgtaaaggg cttgacattg
     9181 atatagtaaa atgaagtaag agcaggacaa attaatcagg acagtcaaat cgatttctaa
     9241 caatgtttta gaagtagagg tgtactattc tagtttcaat ccactataaa atcatgatgc
     9301 acctgtcaga tacttttaac tcatgggact accgtcaggt tgagtattat taggatagag
     9361 aagaagtcta gtgttaagga cattacagct cataatacta ggctttttta tagaaattct
     9421 tttctttttc tataggatat ggtattctat gtagaaaata tatgttaata agtaatgcca
     9481 atatttaaac atcattagta aaaggagtta gattgatgaa taaaagaaaa gttagtttag
     9541 aagattttta taaatggtat agtctaaata aagaagagtt attaaataag gcaactgttg
     9601 gtgaaaagtt taatgataaa ttaaaagaag agtttctcca ggaatggcct ttggatagga
     9661 ttttaacaat gtcaatcgat gaatatgtaa taggaaaggg acagcaaaat aagtctttat
     9721 gctacgctct tgagaaggga aaatacaaaa atctatttct tggaatttct ggtggctcag
     9781 cttcaaaatt tggtatttat tggaataaaa aaacaaacaa atataaagat caagctaata
     9841 atgagatttc agagttggat cagcgatttt caaaattaaa atcagatttg tatgaaatta
     9901 tcaaagaagg tattcgtttt aactttgaaa atcctatttt tgatatgaaa agatcaacaa
     9961 atgaatttat tggtcgttct gctatggtga caaaattact ttgtatctat tctgggggag
    10021 atcctttctt tggtgtaaat attaatagtc agaaagaatt ttggaaccac tttgtttctc
    10081 agacaaatca aggtggacct tatctgcaaa atcataaaat aattgaactg gtgtccaaaa
    10141 cttatcctga gttggagcca tcgaaattag gaactatgct ttttgagtat tctaagcttt
    10201 ttatggaaaa taaggaagac aatagtacaa tggattcatc aaacaatttt cgtcatcaat
    10261 taactcaatc tctattaaag tctccaaacc ttatccttcg tggtgctcct ggcacaggaa
    10321 agacttatct tgctaaagaa attgccaaag aattaacgga tggcaacgaa gaccaaatcg
    10381 gatttgtaca atttcaccca tcctatgatt atacggattt tgtagaaggt ttaagaccag
    10441 tatcaaatgg ggatggagct attgagttta ggctacagga cggtattttt aaagattttt
    10501 gtcagaaagc aaaagaaacc caattgattg gaggacaaga taattttgat gaggcttggg
    10561 attcttactt agaatatata aatgttgctg aagaaaaaga atatataaca aaaacatctt
    10621 acttatctgt taatagtaga caaaatttgt cagtaaatta tgatagtggt gttccaggat
    10681 ggtcactacc tagcaaatat gtttacgagt tgtataaaga taaaaattat aataagcaag
    10741 aatactacaa aagtggtgga aaaactgtcc tagaaacatt gagaaagaga tttggtttga
    10801 aagactatgt ttccccaaca gaaattgata ctgataagaa attcgtcttc atcatcgatg
    10861 aaatcaatcg tggggagatt tctaagattt ttggcgaact ctttttctct atcgaccctg
    10921 gctatcgtgg tgaaaaagga agtgtttcta cccaatatgc aaatctacac gaaactgatg
    10981 aaaagttcta tatccccgaa aatgtttaca tcatcggaac tatgaatgat attgatcgtt
    11041 cagtggatac ctttgatttt gctatgcgtc gtcgttttcg ttttgttgaa gttactgccg
    11101 agggtcaagt tggcatgttg gataaagagt tgaatatcca tgcagaagaa gcaaaaattc
    11161 gtctaagaaa cttgaacgct gctatcgaaa atgttcagga attaaacagt cattatcata
    11221 ttggaccaag ttattttctt aagttgaagg atgtagattt tgactatgaa ttactctggt
    11281 ctgattatat taagcctctc ctagaagact acttgcgagg ttcttatgat gaggttgaaa
    11341 ctttggaaac tttgaaaaaa gcatttgaac tgacaaataa tgatcaaact ggtcaacaag
    11401 atactggtga taatgatgcg gattactgat aatcaataca agattattaa agaaaaattt
    11461 gttgaagaat atcctaaact aagcaatctt cttttagaca gaaccttgga aagtctatcc
    11521 caagatgaac gtattttcat ttttccaaat gatttgactc atactcctga tttggataag
    11581 gaccaaaaga tttttgaaac agtcaatcag aaaatcaaga cagggaacgt gattggtttt
    11641 cttggatatg gtcaggaaag attaacgatt tcctcacgat tttctgatga gagtaatgac
    11701 cattttttgc attatctctt aaacaagatt cttcatatca atctcactag tttagatgtt
    11761 gctttgtctc gtgaagagag gctttatcaa cttttggtgt atctctttcc caagtatcta
    11821 caagctgcta ttcgaaaagg tctttataag gaatatcatc gattttctca taacgacagt
    11881 catgttaagg gagtgattga tgtaagaaac catctcaaga aaaatcttcc tttcacggga
    11941 aatattgcct acgtaacgag agagttcacc tatgataatc ccctcatgca gttggtccgt
    12001 tacactattg aatacattaa gaatcagaaa agcattggtc aaggggtact agataatctc
    12061 tcaactagtc gtgaaaacgt atctgaaatc gtgcgtgtaa caccctctta taaactagct
    12121 gatcgtgcta agattattcg gggaaatcaa tctaaaccta tacgtcatgc atactttcac
    12181 gagtacagaa acttacaaga actttgtctg atgatcctaa accaagaaaa gcacggttta
    12241 gggtatcaag atcaaaaaat ctatggtatt ctctttgatg ttgcctggct ttgggaagag
    12301 tatgtttaca ccttgttgcc aaaaggtttt gtacatccca gaaataagga taagacggat
    12361 ggaatttcag tattttctgt tgggaaacga aaagtatatc cagattttta tgacagagaa
    12421 cgaaagattg ttctagatgc aaaatataaa aaactggaat tgactgaaaa aggaatcaac
    12481 cgtgaggact tattccagct gatttcctat tcttatattt taaaagctga gaaggctgga
    12541 ctgatttttc ctagtatgga gcagtcagta aatagtgaaa taggaaaagt agctggctat
    12601 ggagctcaat tgaagaagtg gtctattcga atccctcaga atgcctcatt ctatagtaca
    12661 ttttgtaaaa tgatggaaaa ttcagaagaa aattttaaag cgattattga tgaagaagtg
    12721 gggagaaaat aatttcctct ctttctttta tttttaacac aagtaaaacc cgaacaatat
    12781 atttcaattc ataaaataat ttgacaaaaa ctgtactttg gtttataata aattaaggaa
    12841 acgtcggaaa agacgtagtg atgcgagagc ataggtaggt cattacaaaa gaaacgagac
    12901 atcgatatgt taaatgaatt tccaattttt gattacgaag atattcaatt gattccaaat
    12961 aaatgtgtca ttaaaagccg tgcagaagcg gatacaagtg tcactttagg aaatcacacc
    13021 tttaaactac ctgttgtgcc agcgaatatg cagacgattt tggatgaaaa tgtagcagag
    13081 caactggcta aaggtggtta cttctacatt atgcaccgtt ttgatgaggc aggacgcatt
    13141 ccttttatta aacgaatgca cgatcaaggg ctcattgctt ctatctctgt cggtgttaag
    13201 gactatgagt atgatttcgt tagacagctc aagactgatg ctcctgagta tatcactatt
    13261 gatattgctc atggtcatgc ggatagcgtg atttctatga ttcaacacat caagaaagaa
    13321 ttgccagata cctttgtcat tgctggaaat gtgggaacac cagaagctgt gcgtgaattg
    13381 gaaaatgctg gtgcggatgc tactaaggtc ggaatcggtc ctggtaaggt ttgtatcacc
    13441 aaggttaaga ctggttttgg tacaggtggt tggcagttgg ctgctctacg ctggtgtgcc
    13501 aaggctgcgc gtaaaccgat tatcgctgat ggaggaattc gtactcacgg tgatattgcc
    13561 aagtctatcc gcttcggtgc tagcatgatc atgattggtt ccctctttgc aggacacatt
    13621 gaaagtccag ggaaaacgat tgaagtcgat ggtgaacagt tcaaagaata ttatggttca
    13681 gcctcacaat atcagaaagg tgcttacaaa aatgtggaag gcaaacgtat cttgcttcct
    13741 gctaaaggtc atctgcaaga cactttaact gagatggaac aagaccttca aagtgctatt
    13801 tcttatgcag gtggacgtca ggttgctgac cttaaacacg ttgattatgt gatcgtgaaa
    13861 aactccatct ggaatgggga tgcttcccac taagacgggg tctttgacta aaaaacttgt
    13921 tattagagca aatttctgtt ataataaaac aagtttccac ccttagtgta atggatatca
    13981 cgtaagattc cggttcttga gatgggggtt cgattccctc agggtggatg taaacatcct
    14041 aaaaagcctt taatagggct tttttcttta cactgctcta aatttgccgc taaaactgag
    14101 aatctctgtc taaagaattc ccaaatgctt tccagtcccg ttttagagtg actcaggggg
    14161 ctttttttga tataataaaa aggactatta tcagttagaa agaggttggt atgaaagaat
    14221 tacaaactgt actaaagaac cattttgcaa tcgaatttac agacaaaaat ttactggaaa
    14281 ctgcctttac tcatacgagt tatgccaatg agcaccgcct cttaaaaatt tcacacaatg
    14341 aacgcttgga atttttagga gacgctgttc tacagttatt gatttcagaa tatctatata
    14401 aaaaatatcc taaaaagcct gaaggtgacc tatcaaaact ccgtgctatg attgtccgtg
    14461 aggagagttt agctggtttt gcgcgtgatt gccagtttga ccagtttatc aagttgggta
    14521 aaggggaaga aaaatctggt ggtcgcaatc gtgacaccat tcttggtgat gcctttgaag
    14581 cctttcttgg tgcccttctt ttggataagg atgtggccaa ggtcaaggaa tttatctatc
    14641 aagtcatgat tcctaaggtt gaagcaggcg agtttgagat gattacagac tataaaaccc
    14701 atctccaaga gttgcttcag gtcaatggtg atgtggctat tcgttatcag gtgatttctg
    14761 aaacagggcc tgctcacgat aaggtctttg atgtagaagt tcttgttgaa ggtaagagca
    14821 tcggtcaagg ccaaggtcgt tctaagaaat tagcagagca ggaagctgcc aaaaatgccg
    14881 ttgagaaggg gctggattca tgtatttaaa ggaaatcgaa attcaggggt tcaagtcttt
    14941 tgctgataag accaaggtcg tttttgacca aggtgtgacg gcagttgttg gacccaatgg
    15001 atctggaaag tccaatatta cagaaagtct gcgttgggct ttgggggagt ctagtgtcaa
    15061 gagtctccgt gggggcaaga tgccggatgt catctttgct ggaaccgaaa gtcgcaaacc
    15121 gctcaattat gcttctgtag ttgtgactct ggataatcat gacggattta tcaaggatgc
    15181 aggtcaagaa atcagggtgg aacgccatat ctatcgtagt ggagatagcg aatacaagat
    15241 tgacggcaag aaagtccgtc tgcgtgatat tcatgacctc ttcttggata ctggattggg
    15301 acgagattcc ttctctatca tttcccaagg gaaggttgag gagattttta attctaagcc
    15361 tgaggaacgc cgagctattt ttgaagaagc tgcaggagtt ttaaaataca agactcgcag
    15421 aaaagaaact gagagtaaac tgcaacaaac tcaggataat ctagaccgct tagaggacat
    15481 tatctacgag ttggataatc aaatcaagcc tcttgagaag caagctgaga atgcccgtaa
    15541 gtttttagac ttggaaggac aacgtaaggc tatttattta gacgttctgg ttgctcaaat
    15601 caaggaaaat aaggcagaac tagagtcgac agaagaagag ctggctcagg ttcaagaact
    15661 cttgatgagt tattaccaaa agcgtgaaaa attagaagaa gaaaatcaaa ctcttaaaaa
    15721 gcaacgccaa gatttacagg ctgaaatggc caaagaccaa ggcagtttga tggacttgac
    15781 tagtctgatt agtgatttag aaagaaaatt agccctatcg aaactggagt ccgagcaagt
    15841 agccctgaat caacaggagg cacaagctcg tttggctact ttggaggata agagaaattc
    15901 actcagcaaa gaaaagtatg ataaagaaag ctctttagct ctgttagagg gaaatctagt
    15961 ccaaaataat caaaaactca atcgtttaga agctgaattg ctggctttct cagacgatcc
    16021 tgatcagatg attgagctct tacgtgaacg ctttgtagct cttttacaag aagaagcgga
    16081 tgtctcaaac cagttgaccc gtattgagaa tgagttggaa aatagtcgtc agctttctca
    16141 aaaacaagca gatcaactag aaaagctgaa agagcaattg gctatagcta aagagaaggc
    16201 tagtcagcaa aaggaagagc ttgaaactgc caaggagcag gttcagaaat tattggctga
    16261 ctaccaagct attgccaagg agcaagagga gcagaaaact tcctatcaag ctcaacaaag
    16321 ccaactcttt gaccgtctgg ataatctcaa aaacaagcag gccagagctc aaagtttgga
    16381 aaatatcctg agaaatcata gtaactttta tgcaggtgtt aagagtgttc tccaagaaaa
    16441 agatcgccta ggtgggatta ttggtgcagt cagtgagcat ctgacctttg atatgcatta
    16501 tcaaactgcc ctagaaattg cgcttggagc gagtagccaa catatcatcg tagaagatga
    16561 agagtcggca accaaggcta ttgacttcct taaacggaac agagccggtc gtgcaacctt
    16621 tcttcctttg accactatca aggcgcgtac gatttctagt cagaaccaag atgctatcgc
    16681 tgtaagccca ggtttccttg ggatggcaga tgagttggtg acttttgata ctagactgga
    16741 agccattttc aagaacttgc tagctacgac ggctatcttt gatactgtag aacatgcgcg
    16801 tgcagcagcg cgccaggttc gttatcaggt tcgtatggtg acattggacg gaacagagct
    16861 gcgcacaggt ggttcttatg caggtggagc taatcgccag aataacagta ttttcatcaa
    16921 gccagaactg gagcaattac aaaaagaaat tgctgaagaa gaagcaagct tgggttcaga
    16981 agaggcgact ttgaagacct tacaagacca gatggctaga ttgtccgaat tattagaagc
    17041 catcaaatct caaggagaac aggcacgtat tcaggagcaa ggcttgtccc tcgcttatca
    17101 gcaaactagt cagcaagttg aagaactgga aactctttgg aaactccaag aagaggaatt
    17161 agatcgtctt tctgacggag attggcaagc agataaggaa aaatgccaag atcgtcttgc
    17221 tactatcgcc agtgacaagc aaaatctgga agctgagatt gaagagatta agtctaacaa
    17281 aaatgccatc caagaacgct atcaaaactt gcaggaagag ctagcgcaag ctcgtttgct
    17341 taagacagaa ctgcaagggc aaaaacgtta tgaagttgct gatattgaac gcttaggcaa
    17401 ggaattggac aatcttaata tcgaacaaga agaaattcag cgcatgctcc aagaaaaagt
    17461 tgacaatctt gagaaggttg atacagaatt gctcagtcaa caggcggaag aatccaaaac
    17521 tcagaaaaca aatctccaac aaggtttgat tcgcaagcag tttgagttgg atgatataga
    17581 aggtcaactg gatgatattg ccagtcactt ggatcaagct cgccagcaga atgaggagtg
    17641 gattcgcaag caaacacgtg ctgaagccaa gaaagaaaag gtcagcgagc gcttgcgcca
    17701 tctacaaaat caattaacag accagtacca gatcagctac acagaagctt tagaaaaggc
    17761 acatgaattg gaaaacctca atctggcaga gcaagaggtg caggatttag agaaggctat
    17821 tcgctcattg ggacctgtca acttggaagc tattgaccag tacgaagaag ttcacaaccg
    17881 tctggacttt ctaaatagtc agcgagatga tattttgtca gcgaaaaatc tgctccttga
    17941 aaccattaca gagatgaatg atgaggtcaa ggaacgcttt aaatcaacct ttgaagctat
    18001 tcgtgagtcc tttaaagtga ccttcaagca gatgtttggc ggaggtcagg cagacttgat
    18061 attgactgag ggcgaccttt taacagctgg tgtggagatt tctgttcaac ctccaggtaa
    18121 gaaaatccag tcgcttaacc tcatgagtgg tggtgaaaaa gctctatcgg ctcttgcctt
    18181 gcttttctcc attattcgtg tcaagaccat tccttttgtc atcttggatg aggtggaagc
    18241 tgcgctggat gaagccaatg ttaaacgttt tggggattac ctcaaccgct ttgacaagga
    18301 cagccagttt atcgtcgtaa cccaccgtaa gggaaccatg gcagcggctg attccatcta
    18361 tggagtgacc atgcaagaat cgggtgtttc aaagattgtt tcagttaagt taaaagattt
    18421 agaaagtatt gaaggatgac aattaaacta gtagcaacgg atatggacgg aaccttccta
    18481 gatgggaatg gacgctttga tatggatcgt ctcaagtctc tcttggcttc ctacaaggaa
    18541 aaagggattt actttgcggt ggcttcgggt cggggatttc tgtctctgga aaaattattt
    18601 gctggtgttc gtgatgacat tattttcatc gcggaaaatg gcagtttggt agagtatcaa
    18661 ggtcaggact tgtatgaagc gactatgtct cgtgactttt atctggcaac ttttgaaaag
    18721 ctgaaaacgt caccttatgt agatatcaat aaactgctct tgacgggtaa gaagggttca
    18781 tatgttctag atacggttga tgagacctat ttgaaagtga gtcagcatta taatgaaaat
    18841 atccaaaaag tagcgagttt ggaagatatc acagatgaca ttttcaaatt tacaaccaac
    18901 ttcacagaag aaacgctaga agctggtgaa gcttgggtta atgaacatgt ccctggtgtt
    18961 aaggctatga caactggctt tgaatctatt gatattgttc tggactatgt cgataagggt
    19021 gtagctattg ttgaattagc taaaaaactt ggtatcacaa tggatcaggt catggctttt
    19081 ggagacaatc ttaatgactt acatatgatg caggttgtgg gacatcctgt agctcctgaa
    19141 aatgcacgac ctgaaatttt agaattagca aagactgtga ttggtcacca taaggaccag
    19201 tcggttatag cttatatgga gggcttataa tggcagatat aaaattaatc gcattggact
    19261 tggacgggac cttgctgact actgataaaa ggctgacgga tcgtaccaag gaaaccttga
    19321 aagctgcgcg tgatcgtggt atcaaggtcg tattgacaac tggtcgtccc ttaaaagcta
    19381 tggatttctt tctccatgag ctagggactg acggtcagga agatgagtat accattactt
    19441 ttaatggtgg tttggttcag aaaaatacag gtgaaatcct tgataagaca gtcttttcat
    19501 atgatgatgt ggcacgcttg tatgaagaaa cagagaaatt atcactgcct cttgacgcta
    19561 tctcagaagg aactgtttat caaattcaat cggaccaaga aagtctttat gccaaattca
    19621 atccagcttt gacttttgtt ccagttgatt ttgaagacct gtctaatcaa atgacctaca
    19681 acaaatgcgt gaccgccttt gctcaagaac ccttggatgc agccattcag aagatttctc
    19741 cagaattgtt tgaccaatat gaaatcttta aatcacgtga aatgttgcta gaatggtcac
    19801 caaagaatgt tcataaagca acaggtttgg caaaactaat tagccatctt ggaattgatc
    19861 aaagtcaagt gatggcctgt ggtgacgagg ccaatgacct ctctatgatt gaatgggcag
    19921 ggctcggtgt tgccatgcaa aacgctgttc ctgaagtcaa ggcagtcgca aatgtggtaa
    19981 ctcctatgac caatgatgaa gaagctgtcg cctgggctat cgaagaatat gtgctaaagg
    20041 agaactaaga tatgggattg tttgaccgtc tattcggaaa aaaagaagaa cctaaaatcg
    20101 aagaagttgt aaaagaagct ctggaaaatc ttgatttgtc tgaagatatt gagcctgcct
    20161 tcacagaagt tgaggaagtt tctcaggaag aagcagaggt tgaaattgtt gaacaagctg
    20221 tgttccaaga agaggaaatc caagacacag ttgaagaaag tcaggattca gagccagctg
    20281 taggggtttc tcaagaagaa atagaagaat taccaaactc agaagaatta ccagaggaag
    20341 agaagcttga gcacgaagga actatagaag aaaataattc tgaaattctt gaagcagaaa
    20401 ggcctcaaac agaagaaact gttcaggaaa aatatgaccg cagtcttaag aaaactcgca
    20461 caggtttcgg tgcccgcttg aatgccttct ttgctaactt ccgctctgtt gacgaagaat
    20521 ttttcgagga actggaagaa ctgctgatta tgagtgatgt tggtgtccaa gtcgcttcta
    20581 acttaacgga ggaactacgt tacgaagcca agcttgaaaa tgccaagaaa cctgatgcac
    20641 ttcgtcgtgt catcattgag aaattggttg agctttatga aaaggatggt agctacgatg
    20701 aaagcatcca cttccaagat aacttgacag ttatgctctt tgttggtgtg aatggtgttg
    20761 ggaaaacaac ttctatcgga aaactagccc accgctacaa acaagctggt aagaaggtca
    20821 tgctggttgc agcagatacc ttccgtgcgg gtgcagtagc tcagctagct gaatggggcc
    20881 gacgagtaga tgttccagta gtaactggac ctgaaaaagc tgatccagcc agcgtggtct
    20941 ttgatggtat ggaacgtgcc gtggctgaag gtatcgatat tctcatgatt gatactgctg
    21001 gtcgtctgca aaataaggat aaccttatgg ctgagttgga aaagattggt cgtattatca
    21061 aacgtgttgt gccagaagca ccacatgaaa ccttcttggc acttgatgca tcaacaggtc
    21121 aaaatgccct agtacaggcc aaagaatttt cgaaaatcac acctttaacg ggaattgttt
    21181 tgactaagat tgatggaact gctcgaggag gtgtggttct agccattcgt gaagaactca
    21241 atattcctgt aaaattgatt ggttttggtg aaaaaatcga tgatattgga gagtttaact
    21301 cagaaaactt tatgaaaggt ctcttggaag gtttaatcta atcagaagca aaaatcctgc
    21361 aaggcataaa cttgcaggaa atttttttat tctaagcgac catcttgacg ataggtgata
    21421 tctggttgcc aagtccattt ggcaccgaat ttttcaagta ggtcaaagct ggcttgaggt
    21481 cccatgcttc cagctttata gtcatgaagt ggggcaccat tttcagccca gagcttttca
    21541 atacggtcaa tcaacttcca tgacgcacaa acttcatccc agtggctaaa gttagttgag
    21601 ttgttattta ggacatcata aatcaatttt tcgtatggtt ctggagaagc accagttgca
    21661 gtcgcatctg tacggtaatc aagtgagtta ggagccaagt taaattcttc tcctacttgc
    21721 ttcccattta ggctaagaga gaagccttct gttggttgaa tatagatggt caaaatattt
    21781 ggagcaagtg gttctccaaa gatagaatcc atttgtttaa agacgatgtt gacatgagtt
    21841 cctttttcag tcagtcgttt acctgtacgg aaaaagaaag gaacaccacg gaatcgatcg
    21901 ctgtctacaa agaaggcacc agatgtaaag gtttcagttg ttgattctgg attcacattt
    21961 ggctcgctac gataagagat gtatttcatg ccatcaatct taccagagcg gtattgccca
    22021 cggataaagt gttctttgag ttcttcgtca gttggatgat agaggttttt aaagacctta
    22081 atcttttcag cacgaatctc gtcttttgtg aagcttgctg gtttgtccat ggcgaggagc
    22141 gaaagaagtt gtagagtgtg gttttggacc atgtcacgga gggcaccgga ttggtcatag
    22201 tagccaccac gttcttctac acccaagcgc tccgcaaagg taatttgaac attgtcgata
    22261 aaatccttgt tccaaacgtt ttcaaaaatc aagtttgcaa agcgaactgc aaagatgctt
    22321 tggatcattt ccttaccaag ataatggtcg atacggaaaa tttgttcttc gtcaaatgtt
    22381 gctaggagtt cgtcattcaa cttgcttgca gttgcgtaat ctgtaccaaa tggtttttca
    22441 acgatcaagc gctcaaaacc tttgccatcg acaatgtttt cagatttgag gtgtttggca
    22501 atggttccaa agaactgagg tgccatagac aagaagaaga gcttattgtg ttcagcttgg
    22561 tatttttcat taagctcagc ttgtaattga cgcaaagcaa tataatgttc cgaatcattg
    22621 acatcatggc tttgatagta gaagtggcta gcaaattctt gggcttgctc ggtactatct
    22681 gccaaatcaa ggatggactc gacaactaca gattcaaaat attccttact ccaaggtcta
    22741 cgggcagttc caataacggc aaagtgcttg gaaagattgc cggatttata tagtctaaaa
    22801 agggaagggt agagcttgcg tttagccagg tctccactcg caccgaaaat tgtaacaata
    22861 accttagatg acatctagct acctaatttc tatttttatt cctagtcttg ctaggtatga
    22921 ggaaacctca tttcataaac ttaattctaa cataattttc cgaaaaatca aaaattccaa
    22981 tacgaagtag aaaaactgca aggaaatcct tacagtttga gtttagacgt ttaagacctt
    23041 atctaagaac tctttcagac gagggtgttg tgggttatca aagatttggt caggtgttcc
    23101 gtcttcaagg aactcgccat ctgcagtaaa gataacgcgg ttggcaacct gacgagcaaa
    23161 tcccatctca tgggttacga taatcatggt catgccttgc tcagccaatt ccttcataac
    23221 gttaagtacg tctccaacca tctcagggtc aagggcagaa gttggttcat cgaagagcat
    23281 gatgtctgga ttcattgcta ggccacgagc gatggccaca cgttgttttt gaccacctga
    23341 taggctatct ggattggcat tagctttatc tgctagtcca accttttcaa gcaactccat
    23401 tcccaattcc tcagcttctt ccttagtcat caacttgtgc tcaataggag caaaggtgat
    23461 gttgtccaat acagacatat gagggaagag gttgaagtgt tggaatacca tgccgatatt
    23521 ttcacggacg tggtcaacat tggttgtttt ttcagttaaa tcatagccgt tcacagtgat
    23581 gtgaccgcta gtgacttctt ctaaaagatt gaggctacgg aggaaagttg acttaccaga
    23641 acctgaagga ccgatgatac aaacaacatc tccttcatag aacttagtcg taattccttt
    23701 taggacttca ttttttccat agtgcttgtg taaatcattt acatcaattt ttaattttgc
    23761 cattaacgaa tcctcttttc taagcgtttc gctagtctag tcaaaagtgt gataattaca
    23821 agatagaaga tagcaaggat tgcatacatc ttgaaacttt ggtagttacg agcaatgata
    23881 atcttaccag tttggaagag ttcaaccaaa ccgatagcag atacgatagt tgtatcttta
    23941 agagcgataa cgaattggtt gacaaagttt ggcaacatca atttagttgc ttgtggcaag
    24001 ataatcttac gcatggtttt tccataagag atacccaagc ttcggctggc ttccatttgg
    24061 ccaactggaa cggcctgaat accaccacga acgatttcag cgatataagc agccgcattg
    24121 agtgagaggg caatggttcc agctacaaag tcgttaattg ggctttgttg gcctgtgata
    24181 gactcgatga agtttggaat tccccagaag atgaaggctg caagaatcat caatggaata
    24241 ccacgaataa cgtcaacgaa aatctcagag atgatgcgaa gagatttgta tgggctaacg
    24301 ctaaacatac cgaagataat tccgatgaca atggcaatag caaatgagat aagagctaga
    24361 gcaagagtga taccaagacc gctaaggagt tgtttgtagt tgttttgaag caagccccag
    24421 agcgttgttt cgtcaacagt acttgttgaa gcagttgaag attcgctagc taggtatttg
    24481 tcaagaatct tttggaattc accgtttgct ttaaggtttg caagtccgtt gttgaacatt
    24541 tcaatcagtt ctggatttgc tcctttttta acagcaaagg ctgtttcacc gattggagtt
    24601 ccagagattg gagttttcaa tttttgacct tggctgatag aatatttgag aacaggttca
    24661 tcatccataa cggcatcaat ggcaccagtg tttaaactgt catacattga agaaccatca
    24721 gcaaaggttt tgattttgta accgtatttg ctttgatttt ctgttaggaa ggtttgagaa
    24781 gcagttccgt ttttaacacc gactgtcttt ccttttagat cttcataaga agcaatattg
    24841 cttgattctt tgacaccaag aatggtatta gcagtgtagt atgattctga gaagtcaaaa
    24901 gttgccttac gagcatctgt gacagacata ccagcaatga taccatcggc ttgaccagct
    24961 tggacagcac tgatagcagc atcaaaacca gggttggtga tttcaatttc aaaaccttgg
    25021 tctttagcga ttgccttaat caattccata tcaataccag tgtattggtt gcttgaattt
    25081 tggaaaacaa aaggtgcaaa agaagaatcg ctggcaatga tatatttagc cttaacagga
    25141 atagctttta atcctgcgag agtagttgta gttggcactg ctgaagatga tgaagcagtc
    25201 catttcttga taattttatc aagactacca tcttttttca tttcagacaa ggcttggtta
    25261 aattcagtaa ccaggtgctc gtatttactt ccttttttca caccgaaagc aaaacttcct
    25321 acagcttcac catccatttc aatatggagg tcttgacctt ggttaatggc atattcgata
    25381 acaggtttgt catccatcat ggcatcgatg gcaccagcac tcaagctgtt gttcattaaa
    25441 tcaccagtgt caaatgtttt aatagtaaag ccgtatttat ctttgattgt ttcaaggaaa
    25501 cgttgagcgg cagttccgtt tttaacacca acggttttgc cagttaattg gtcgtacttg
    25561 ctaattttgt gtgactttgt agtagcaatg acaacttttg tatcatagta agtatcagac
    25621 atggtgaaga ctttttcacg ttctttagtc tttgtcatcc ctgccatgat agcgtcggct
    25681 tgcccagctt gaaccgcatt gactgctgcg tcaaatccag gataggacat ctgaatgttc
    25741 cagcctttaa tctcagcgac tttgttaata atgtcaacat caattccttt ataagtttga
    25801 tctgaatctt taaactcaaa aggtgcatag gcagtatcag aaacaatctt aatcgtttct
    25861 gctttggcaa tacctaatga gaaaattggg aataaaatta gcaaaaatgc tagaaatttt
    25921 ttcttcattt ttagtctcct tttccgaata ttctctcatt atatcagaaa aagtaaaaaa
    25981 aggcaaatgt tatatttttc gtgtcagaaa atataacatt gatttttcct tttctttctt
    26041 gaattgctat taaaaagtgc tataataata gtatatatag aagaaaagag gacggggata
    26101 tgaagaacaa aagaatattt aaagacttcc aagcttcaaa aatgagttta aacatttaca
    26161 caagcccctt gttagccttt gtttttgtct tcataggaga gtttgtggct tatactttgt
    26221 atggtattgg cttgttagct ctcatcggac ttgctagaaa ttttggagag gctggtcaaa
    26281 atcttgcaac ctacttgcag accttgcatc agagcttgac ggataaaaca agtgactttc
    26341 gtttaatttt aggattactg gcctttggtt ttattcttaa cactgtgttc agatggacaa
    26401 gaaaagttga gaaaagacct attcgaacct tgggatttta tagagagaat ttcctcagca
    26461 atcttctgaa aggatttagt ctaggtctgg cactttttct tctgaccttg ttaggtttag
    26521 tggtcttagg tcaatatcgt ttggaatcca ttcacttgaa tccttattct cttgcctttg
    26581 tcgtctttac tatcccattt tggattttac aggggacagc agaagaagtg gtggcccgtg
    26641 cttggctact tcctcaattg gcctcaagaa ccaatctaaa actagctatt cttatatcta
    26701 gcctgttctt taccctgctt catatgggca attttggcct cacccctcta tctctagtaa
    26761 atctcttttt attcggagtt gccatggctc tttaccttct caaaactgat acagtttggg
    26821 gtgttgcagg tattcatggt gcttggaatt ttgctcaggg taatctcttt ggtattttag
    26881 ttagtggtca accgtcagga acgtctctga tgaccttttt accacaaggc aatcaagatt
    26941 ggctatcagg tggttctttt ggcatagaag gttccattat gacaagtctg gtattactac
    27001 tgctgattgt ctatcttgct aataaattaa agaaagaaaa tgaaaggatg tgacttcggt
    27061 ccgtcctttt cttcgtgaaa atactataag tatgctaaaa taggaatagc acatggagag
    27121 aggattctta tgatcaatca cattacagat aatcaattta aactagtatc aaaatatcaa
    27181 ccatcaggag atcaacccca agctatcgag cagttggtgg ataacattga ggggggagaa
    27241 aaagctcaga ttctgatggg ggcgactgga acagggaaga cctatactat gagtcaggtc
    27301 atttctaaag tcaataaacc aactctggtt attgcccaca ataaaactct ggctggtcag
    27361 ctctatgggg agtttaagga atttttccct gaaaatgcag ttgagtattt cgtatcctac
    27421 tatgattatt accagccaga ggcctatgtc ccttctagcg atacctatat tgagaaggat
    27481 agttctgtca atgacgagat tgacaagctt cgccactcag ctacctcagc ccttttggag
    27541 cgtaatgatg ttattgtcgt ggcctcagtc tcttgtatct atggtttggg ttcgcccaag
    27601 gaatacgctg atagtgtcgt tagtctccgt cctggtctag agatttctcg tgataaactc
    27661 ttgaatgact tggtcgatat tcagtttgaa cgtaatgata ttgatttcca acgcggaaga
    27721 tttcgcgttc gtggggatgt ggtagagatt ttcccagctt cccgagatga acatgccttt
    27781 cgagtagaat tttttggaga cgaaattgac cgtatccgtg aggttgaggc tctgacaggt
    27841 caggtattgg gagaggtgga tcatttggcg attttcccag ctactcactt tgtgaccaat
    27901 gacgaccata tggaagttgc cgttgccaag attcaagcag aattagaaga gcagttagct
    27961 gtctttgaga aggaaggtaa attgcttgaa gcccagcgct tgaaacaacg gacagagtac
    28021 gatatcgaaa tgctgcgcga gatgggctat acaaatgggg ttgaaaatta ttctcgccac
    28081 atggatggac gtagtgaagg tgagcctcct tatacgcttc tcgacttttt cccagatgat
    28141 tttttgatta tgattgacga gagtcatatg actatgggac aaataaaagg catgtacaat
    28201 ggagaccgtt cgcgtaagga aatgctagta aattatggtt tccgtttacc gtctgccttg
    28261 gacaatcgtc ctctccgtcg tgaggagttt gaaagccatg tgcatcagat tgtttacgta
    28321 tcagcgacac ctggtgacta tgaaaatgaa cagactgaga cggtgattga gcaaatcatt
    28381 cgaccgacag ggcttttgga tcctgaggtg gaagtccgtc cgactatggg acagattgat
    28441 gatctcttag gtgaaatcaa tgcccgtgtt gaaaagaatg aacgaacctt tatcaccacc
    28501 ttgaccaaga aaatggcaga ggacttgacc gactatttca aggaaatggg catcaaggtc
    28561 aagtacatgc actcggatat caagaccttg gaacggacgg agattatccg tgatctccgc
    28621 ttgggtgtct ttgatgtctt ggttggaatc aatctccttc gtgaagggat tgacgttcct
    28681 gaggttagct tggttgccat tctcgatgct gacaaggaag gtttccttcg taacgagcgt
    28741 ggactcatcc agaccattgg gcgtgccgcc cgtaacagtg aaggtcatgt catcatgtat
    28801 gcagatacgg ttacccagtc tatgcaacgt gctatcgatg aaactgcccg ccgtcggaaa
    28861 attcagatgg cctataatga agagcatggt atcgtaccac agacaatcaa gaaagaaatc
    28921 cgtgacctga ttgccgtgac caaggcagtt gctaaggaag aggacaagga agtcgacatc
    28981 aatagcctca acaaacaaga gcgcaaagaa ctcgtcaaga aactagaaaa acaaatgcaa
    29041 gaagccgtcg aagtgcttga ctttgaactg gcggctcaga tccgtgatat gatgttggaa
    29101 gtcaaggcct tggattaggg gaatagtatg atttatttaa gaaagttaaa gaaagaagat
    29161 ttgatgtctt tatgggaaat ggcttattca cagcttaatc cagtttggaa acagtatgat
    29221 gctccctatt atgatgatta tcagtatttt tcaaatttta aagaattcga actacaaaaa
    29281 tcagaatcca ttttaagcaa ctcaaatcgc cttggtattt ttgttgatga taaactagtt
    29341 gggactgttt cgcgttattg ggtatgtaaa gaaacaagat ggatggaatt gggaattggt
    29401 atttatgata aaaaattctg gaacactggt attgggaaag ttgctatgtt gcagtggata
    29461 gataggacgt ttcaggatta cttggagttg gagcatctgg ttttgacaac ttggtcagga
    29521 aatcttggta tgatgaaact tgctgaaaaa ttaagaatga aaaaagaagc tcatattcca
    29581 aaagttcgtt attatcaagg taaatatttt gatagtatta aatatggtat tttgagagaa
    29641 gactgggaga aaataaatga cggttattat caaatcaatg gaaactcctg aagagataga
    29701 aggcaaatcc ctcgttcact ggcaaacgtg gagagagtct tatgatgacc ttttgcctgc
    29761 ggaatttcag gatacaatga cattagaaag atgtcgattc tttagtcaaa agtatccaga
    29821 aaatacattg attgcgatgg atggcgtgaa ggtggttggt tttatcagtt atggcaattt
    29881 tcgtgatgag actattcaag ctggtgaaat tattgcttta tatgttttaa aagactatta
    29941 tggaaaaggt atcgcacaaa agttaatgaa agcagctttg actgctctta atcatttttc
    30001 tgaaattttc ttatgggtat tgaaagataa caagcgcgcc attgctttct atcaaaaaat
    30061 gggttttact tttgatggac aagaaaaaat acttgaactt ggaaagccta taaaggaaaa
    30121 acgaatggtg ttctattcta aataatttta aaaaagtaaa agataatatc tctggtagta
    30181 agtctgaaaa tttaataaat tagaaagcga gtaaatttat gtcccgttcc caattaacaa
    30241 ttttaacaaa tatctgtctg attgaagacc tcgaaactca gcgcgtggtg atgcagtatc
    30301 gcgcccctga aaacaatcgc tggtctggtt atgcctttcc tggaggtcat gtagaaaatg
    30361 atgaggcttt tgcggagtct gtcattcgtg aaatctacga agaaacaggg ttgactatcc
    30421 aaaatcctca acttgtcggc attaaaaatt ggccactaga tacaggtggg cgctatattg
    30481 tcatttgtta taaggcgact gagttctctg gtacccttca atcttcagaa gagggagaag
    30541 tttcttgggt gcaaaaagac cagattccaa acttaaatct ggcctatgat atgctaccat
    30601 tgatggaaat gatggaagct cccgacaagt cagagttttt ctatcctcgc cgtacagatc
    30661 taactttccc atctttgaga tagcccttca caagatagac atgagtacgc ctttctttgt
    30721 tgttttaata aacgctctac tccattactc catatataac caatatacaa caaatagttg
    30781 ggaacaaact gtggtagatg tcgaacgtct tcgtgatgag gctatagctc taggagtagc
    30841 ttaatgacaa agagacagta ggagaggtac gattatgtta ctctttatgt tttcgttcaa
    30901 taatataata ataaatccta gtatgttcct attgttcgat agtacaggag cttactatcg
    30961 gcacatgttg agacggtagt attgatgtca aaagttgtac caactaagta aataaagtgt
    31021 agaaattatg gcttattcta aggctatcgt taaaaggtat cggtagtttg gagtaagcct
    31081 tgttttttta tttatcttgc caagattaca ggattagttg caggtgtctt cagagatata
    31141 tttgtaggaa gggatttgtt tttaattaaa acataattct attacccaga tatattttga
    31201 attaatatgt tcacaatagt gaaaaacaag caat
//
LOCUS       DBERMR010000025        30296 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.025, whole genome shotgun sequence.
ACCESSION   DBERMR010000025 DBERMR010000000
VERSION     DBERMR010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 30296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30296
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.025"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(14..655)
                     /gene="plsY"
                     /locus_tag="ACL8A1_001194"
     CDS             complement(14..655)
                     /gene="plsY"
                     /locus_tag="ACL8A1_001194"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HGS1201970.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            791..2734
                     /gene="parE"
                     /locus_tag="ACL8A1_001195"
     CDS             791..2734
                     /gene="parE"
                     /locus_tag="ACL8A1_001195"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HGS1201971.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTNE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVVNTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            2749..2953
                     /locus_tag="ACL8A1_001196"
                     /pseudo
     CDS             2749..2953
                     /locus_tag="ACL8A1_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014166.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
     gene            3154..5625
                     /gene="parC"
                     /locus_tag="ACL8A1_001197"
     CDS             3154..5625
                     /gene="parC"
                     /locus_tag="ACL8A1_001197"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HGS1201972.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLV
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEAILYVEVLDQFDDATTYFAATRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            5755..6777
                     /locus_tag="ACL8A1_001198"
     CDS             5755..6777
                     /locus_tag="ACL8A1_001198"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HGS1201973.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGIGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            6833..7537
                     /locus_tag="ACL8A1_001199"
     CDS             6833..7537
                     /locus_tag="ACL8A1_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS1201974.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKYIK"
     gene            7602..8291
                     /locus_tag="ACL8A1_001200"
     CDS             7602..8291
                     /locus_tag="ACL8A1_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0858 family membrane protein"
                     /protein_id="HGS1201975.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            8288..9211
                     /locus_tag="ACL8A1_001201"
     CDS             8288..9211
                     /locus_tag="ACL8A1_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0859 family membrane protein"
                     /protein_id="HGS1201976.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            9226..9870
                     /gene="pcp"
                     /locus_tag="ACL8A1_001202"
     CDS             9226..9870
                     /gene="pcp"
                     /locus_tag="ACL8A1_001202"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HGS1201977.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            10038..10268
                     /locus_tag="ACL8A1_001203"
     CDS             10038..10268
                     /locus_tag="ACL8A1_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HGS1201978.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            10318..10398
                     /locus_tag="ACL8A1_001204"
     tRNA            10318..10398
                     /locus_tag="ACL8A1_001204"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10352..10354,aa:Tyr,seq:gta)
     gene            10408..10479
                     /locus_tag="ACL8A1_001205"
     tRNA            10408..10479
                     /locus_tag="ACL8A1_001205"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10440..10442,aa:Gln,seq:ttg)
     gene            10641..11843
                     /gene="rpsA"
                     /locus_tag="ACL8A1_001206"
     CDS             10641..11843
                     /gene="rpsA"
                     /locus_tag="ACL8A1_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HGS1201979.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPVQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            11929..12426
                     /locus_tag="ACL8A1_001207"
     CDS             11929..12426
                     /locus_tag="ACL8A1_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HGS1201980.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
     gene            12426..14081
                     /gene="dnaX"
                     /locus_tag="ACL8A1_001208"
     CDS             12426..14081
                     /gene="dnaX"
                     /locus_tag="ACL8A1_001208"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HGS1201981.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIQEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNMFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNAGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            14109..14303
                     /locus_tag="ACL8A1_001209"
     CDS             14109..14303
                     /locus_tag="ACL8A1_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HGS1201982.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            14453..15223
                     /gene="sufC"
                     /locus_tag="ACL8A1_001210"
     CDS             14453..15223
                     /gene="sufC"
                     /locus_tag="ACL8A1_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HGS1201983.1"
                     /translation="MSVIEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELKVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            15251..16513
                     /gene="sufD"
                     /locus_tag="ACL8A1_001211"
     CDS             15251..16513
                     /gene="sufD"
                     /locus_tag="ACL8A1_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HGS1201984.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            16524..17750
                     /locus_tag="ACL8A1_001212"
     CDS             16524..17750
                     /locus_tag="ACL8A1_001212"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HGS1201985.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKVINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHVSNVLSVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            17737..18177
                     /gene="sufU"
                     /locus_tag="ACL8A1_001213"
     CDS             17737..18177
                     /gene="sufU"
                     /locus_tag="ACL8A1_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HGS1201986.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQDQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            18231..19643
                     /gene="sufB"
                     /locus_tag="ACL8A1_001214"
     CDS             18231..19643
                     /gene="sufB"
                     /locus_tag="ACL8A1_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HGS1201987.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(19842..21083)
                     /gene="pbp3"
                     /locus_tag="ACL8A1_001215"
     CDS             complement(19842..21083)
                     /gene="pbp3"
                     /locus_tag="ACL8A1_001215"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase PBP3"
                     /protein_id="HGS1201988.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            21186..22295
                     /gene="cozE"
                     /locus_tag="ACL8A1_001216"
     CDS             21186..22295
                     /gene="cozE"
                     /locus_tag="ACL8A1_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell elongation protein CozE"
                     /protein_id="HGS1201989.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVIFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
     gene            22829..23176
                     /gene="ssrA"
                     /locus_tag="ACL8A1_001217"
     tmRNA           22829..23176
                     /gene="ssrA"
                     /locus_tag="ACL8A1_001217"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(23359..23446)
                     /locus_tag="ACL8A1_001218"
     tRNA            complement(23359..23446)
                     /locus_tag="ACL8A1_001218"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23410..23412),aa:Ser,seq:gga)
     gene            23609..24349
                     /locus_tag="ACL8A1_001219"
     CDS             23609..24349
                     /locus_tag="ACL8A1_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGS1201990.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            24346..25257
                     /gene="pfkB"
                     /locus_tag="ACL8A1_001220"
     CDS             24346..25257
                     /gene="pfkB"
                     /locus_tag="ACL8A1_001220"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HGS1201991.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNISNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVEPVKLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            25254..27206
                     /locus_tag="ACL8A1_001221"
     CDS             25254..27206
                     /locus_tag="ACL8A1_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HGS1201992.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIEEMIKSLTGHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALTELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLAISGDAEVYRAANGAK
                     VATASNEKQSLGGAFYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALSVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            27343..29595
                     /locus_tag="ACL8A1_001222"
     CDS             27343..29595
                     /locus_tag="ACL8A1_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HGS1201993.1"
                     /translation="MERKEAIQRMLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGS
                     LAYLAIFGLLIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSILKGT
                     MAQVVTDLTGFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWS
                     VYDIAEFFSRGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPP
                     ADMETGEILTEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDQDVQVDFSAK
                     EALEYKLPSLQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVT
                     KYEVKPAVGVRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFR
                     ELWEQSQTKAENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASI
                     LMKARPDQVKFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYEL
                     FAKVGVRNIAGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRL
                     GQKARAAGIHMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLL
                     GRGDMLFKPINENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGE
                     FSDGDAGGDPLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEMAGVIGPAE
                     GTKPRKVLQQ"
     gene            complement(29792..30220)
                     /locus_tag="ACL8A1_001223"
     CDS             complement(29792..30220)
                     /locus_tag="ACL8A1_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6556 family protein"
                     /protein_id="HGS1201994.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTITTIGSILGLITAGITIMN
                     ALDNNNKNTKKEPTTSQTTTFVKEIQKESPQENTTPNKENNTSQEKTQQEETPKSSVK
                     EEKKEDQKTATQDSTTPATSKPATENEKQPNTPTSENNTQ"
BASE COUNT         9046 a         5502 c         6774 g         8974 t
ORIGIN      
        1 cagaactggc gttttatttt ttaggatctt gatgggttag gttcaatccc caagggacca
       61 aattttcagt tttatttttg atacgagcta tattgtcctt atgacgaatg ataatcaaac
      121 tagcaagtgc taagataata gcgatgaaga gagagtcata gttactcagg ataaaaccaa
      181 aaagtggaaa gagcagaacc ccgataacag ccgcgatcga tgctgtgaca ctagacagtg
      241 aaatcatact gccaagatag agagctccaa agaagataat cgcaaggtag agacagaaga
      301 taggcgcaaa tccgaaaatc actccagcac tggttgcgac agccttacca cctttaaatc
      361 ctgcaaagat agggaaggta tggccgataa cagccaaaag tccaaagatg agaggagaaa
      421 cgccttgtag atgaaaaata atcggaagca gcgttgctag ggttcctttg aaaaagtcaa
      481 tcacaaaggt tgccatacca gctttcttac ctaaaatgcg gaaggtattg gtcgttccag
      541 tgttaccaga accatgctcg cgtagattga tttgaaagaa tacttgtcca atccagagac
      601 cagatggaat cgaacccagc agataggcta ggattaataa aactattgta atcatactcc
      661 tattatatca tgaattggga aagaaaagca gagaatctct gctgaaattg tcacatcgga
      721 gaaagaaaaa ttttgcaaaa tccttggaaa acctgtagaa tagtaaagat gaacgaatag
      781 gaggttcctt gtgtcaaaaa aggaaatcaa tattaacaat tataatgatg atgctattca
      841 ggtgctagaa gggttggatg cggtccgaaa acgtccaggg atgtatattg gatcgaccga
      901 tggcgctggt cttcatcacc tagtttggga aatcgttgat aatgcagtcg atgaagcctt
      961 gtctgggttt ggtgatcgta ttgatgtaac tatcaataaa gacggtagtc taacggttca
     1021 agaccatgga cgtgggatgc cgacaggtat gcacgctatg ggaattccaa ctgttgaggt
     1081 tatctttacc attcttcatg ccggagggaa attcggtcaa ggtggctata agacatcagg
     1141 tggacttcac ggagtgggtt cttccgttgt taacgccctt tctagctggt tagaagttga
     1201 aattacccgt gatggcgcag tttacaagca acgtttcgaa aatggtggaa aacctgtcac
     1261 gactttgaag aaaatcggta cagcacccaa gtctaaaaca ggcaccaaag ttacttttat
     1321 gcctgacgcg actatatttt ctacgacaga tttcaagtac aataccattt cagagcgcct
     1381 taatgaatca gcctttctct tgaaaaatgt gaccttgtct ttaacggaca agcgaacaaa
     1441 tgaagcgatt gagttccact atgagaatgg agtacaagat tttgtttctt atctcaacga
     1501 agataaggaa atcttgacgc cagttcttta ctttgaaggg gaagacaatg gttttcaagt
     1561 ggaagtagcc ctccagtaca atgacggatt ctcagataac attctatcct ttgtcaataa
     1621 cgttcgcacc aaggacggtg gaacgcacga gacaggactc aagtctgcca ttaccaaggt
     1681 catgaatgac tatgcacgta aaacaggtct tctcaaggaa aaagataaaa accttgaagg
     1741 ttcagactat cgtgagggac tagcggccgt tctttctatc ttagttcctg aagaacactt
     1801 gcagtttgaa ggacagacca aggataaact aggaagcccc ctagctcgcc cagttgtgga
     1861 tggaatagtg gctgataagt tgaccttttt ccttatggaa aatggggaat tagcttctaa
     1921 cctcatccgc aaggctatca aggcccgtga tgctcgtgaa gcagcacgta aggcgcgtga
     1981 tgagagccga aatgggaaga aaaacaagaa agataagggc ttgttgtctg ggaaattgac
     2041 cccagcccaa tctaagaatc ctgctaagaa tgaactctat ctagttgagg gggactctgc
     2101 cggtggttct gccaaacaag gtcgtgaccg caagttccag gctattctac ctcttcgtgg
     2161 taaggttgtc aatacagcca aggccaagat ggcggatatc ctcaaaaatg aagagatcaa
     2221 taccatgatt tataccattg gtgcgggtgt tggagcagac ttctctattg aagatgccaa
     2281 ctatgataag atcattatca tgaccgatgc ggataccgac ggtgcccata tccagacctt
     2341 gctcttgaca tttttctacc gttacatgcg tccgctagtc gaggcaggtc atgtctatat
     2401 tgccctccca cctctttaca agatgtccaa aggtaaaggc aagaaagaag aagtggccta
     2461 cgcttggacg gacggagaac tagaagaact ccgtaaacag ttcggtaaag gcgctaccct
     2521 ccaacgatac aaaggacttg gtgagatgaa tgcggaccag ctctgggaaa caaccatgaa
     2581 cccagaaaca cgtaccctca tccgtgtcac aattgaagat ttagcgcgcg ccgaacgccg
     2641 cgtcaatgtt ctcatgggag ataaggtaga accacgccgt aaatggattg aagataatgt
     2701 caagtttacg ctagaagaag cgacagtgtt ttaatgaaag aaaataatat gttagtaaac
     2761 tggaaattag agtcggatgt gaatgactat gtgaaaaagc agtttgaaaa tcttggtttg
     2821 aaaaaattgc aggattatta atgaagaatc tgctatgagt gcttatctca aagaggcgtt
     2881 taagggagcg gctaagacac aaactaagac taattttgga aaaccatatt ttcatattga
     2941 aaaatacaaa caggatagtt tggttactta tcgtaactga acacgggacg aattcccgta
     3001 aaatatcttt tactgcataa aaacctactc tacattcttt gaaaggagtt gaacacgccc
     3061 tagatactgt gtgaaaaaga taaattctct tgtgagtttg cttacttcaa gaattttcta
     3121 ttttcacttg gtattttatg ggctttgtat cttatgtcta acattcaaaa catgtccctg
     3181 gaggacatca tgggagagcg ctttggtcgc tactccaagt acattattca agaccgggct
     3241 ttgccagata ttcgtgatgg gttgaagccg gttcagcgcc gtattcttta ttctatgaat
     3301 aaggatagca atacttttga caagagctac cgtaagtcgg ccaagtcagt cgggaacatc
     3361 atggggaatt tccacccaca cggggattct tctatctatg atgccatggt tcgtatgtca
     3421 cagaactgga aaaatcgtga gattctagtt gaaatgcacg gtaataacgg ttctatggac
     3481 ggagatcctc ctgcggctat gcgttatact gaggcacgtt tgtctgaaat tgcaggctac
     3541 cttcttcagg atatcgagaa aaagacagtt ccttttgcat ggaactttga cgatacggag
     3601 aaagaaccaa cggtcttgcc agcagccttt ccaaacctct tggtcaatgg ttcgactggg
     3661 atttcggctg gttatgccac agacattcct ccccataatt tagctgaggt catagatgct
     3721 gcagtttaca tgattgacca cccaactgca aagattgata aactcatgga attcttgcct
     3781 ggaccagact tccctacagg ggctattatt cagggtcgtg atgaaatcaa gaaagcttat
     3841 gagactggga aagggcgcgt ggttgttcgt tccaagactg aaattgaaaa gctaaaaggt
     3901 ggtaaggaac aaatcgttat tactgagatt ccttatgaaa tcaataaggc caatctagtc
     3961 aagaaaatcg atgatgttcg tgttaataac aaggtagctg ggattgctga ggttcgtgat
     4021 gagtctgacc gtgatggtct tcgtatcgct atcgaactta agaaagacgc taatactgag
     4081 cttgttctca actacttatt taagtacacc gacctacaaa tcaactacaa ctttaatatg
     4141 gtggcgattg acaatttcac acctcgtcag gttgggattg ttccaatcct gtctagctac
     4201 atcgctcacc gtcgagaagt gattttggcg cgttcacgct ttgacaaaga aaaggctgag
     4261 aaacgtctcc atatcgtcga aggtttgatt cgtgtgattt cgattttgga tgaagtcatt
     4321 gctcttatcc gtgcttctga gaataaggcg gacgccaagg aaaacctcaa agttagctat
     4381 gattttacgg aagaacaggc tgaggctatc gtaactttgc aactgtaccg tttgaccaat
     4441 accgatgtgg ttgtcttgca ggaagaagaa gcagagcttc gtgagaagat tgctatgctg
     4501 gtggctatta tcggtgatga aaggactatg tacaatctca tgaagaaaga acttcgtgag
     4561 gtcaagaaga aatttgcaac tcctcgtttg agttctttag aagacactgc gaaagcaatt
     4621 gagattgata cagctagtct tatcgctgag gaagatacct acgtcagcgt gaccaaggca
     4681 ggttacatca agcgtaccag tccacgttcc tttgcggctt ccaccttgga agaaattggc
     4741 aagcgtgatg atgaccgttt gatttttgtt caatctgcca agacaaccca gcacctcttg
     4801 atgttcacaa gtcttggaaa tgtcatctac agaccaatcc atgagttggc agatattcgt
     4861 tggaaggaca tcggagagca tctgagccaa accatcacaa actttgaaac gaatgaagca
     4921 atcctttatg tggaagtact ggatcagttt gacgatgcga caacctactt tgcagcgact
     4981 cgccttggtc aaatcaaacg ggtagagcga aaagaattca ctccatggcg gacctataga
     5041 tctaagtctg tcaagtatgc taagctcaaa gacgatacag atcagattgt agcagtggct
     5101 ccgattaaac tagatgatgt tgtcttggtt agtcaaaatg gttatgccct gcgtttcaat
     5161 atcgaagagg ttccggttgt cggtgctaag gcagcaggtg tcaaggctat gaatttgaaa
     5221 gaagatgatg tcctccaatc tggctttatc tgtaatactt cgtccttcta cctcttgacc
     5281 cagcgtggaa gcttgaaacg tgtttccatt gaggaaattc tagcaaccag ccgtgccaaa
     5341 cgaggattac aagtcttgcg tgagttgaaa aacaaaccgc atcgtgtctt cttggcagga
     5401 gcagttgcag agcaaggatt tgttggcgat ttcttcagta cggaagtgga tgtgaacgac
     5461 caaactctgc ttgtccaatc caataaagga acaatctatg aaagccgatt gcaagacttg
     5521 aacttgtcag aacgcactag caatggaagc ttcatttctg acacgatttc agatgaagaa
     5581 gtttttgacg cttatcttca ggaagtagtt actgaagata aataagtgaa atgaagaatc
     5641 agttctaaga tggtttttat tggagaaatt ttctgaaaat tacaaaatat acttgctatt
     5701 ttagaaaaat agtgtagaat ataagaaata ggtttttaga ataaggagtg gaatatgaca
     5761 gtaacgattg attgggaaaa cctcggtttt tcctatatga aattacctta tcgctatctt
     5821 gctcatttca aaaatggaca atgggatcaa ggagagctta cagaggatgc aactttgcat
     5881 atttcagagt cttctccaag tcttcactat ggacaacaag catttgaagg tttgaaagct
     5941 tatcgtacta aggatggcag tgttcaactg ttccgtcctg atgaaaatgc taaacgcctg
     6001 caacgtacat gtgaccgtct cttgatgcca caagttccga cagacatgtt tgtagaagct
     6061 tgtaaagcag ttgtccgtgc gaatgaagaa tacgtaccac catacggaat aggtggaact
     6121 ttatatcttc gccctctttt gattggtgtc ggagatatta tcggggtaaa accggcagaa
     6181 gagtacattt tcaccatctt tgctatgcca gttggaaatt actttaaagg tggtttggtc
     6241 ccaaccaact tcttgattca ggatgagtac gaccgtgcag caccaaatgg tacaggtgcg
     6301 gctaaggttg gtggaaacta tgctgcaagt ctcttaccag gaaaaatggc caagtcacgc
     6361 catttctcag atgttatcta tctggaccca tcaactcata caaagattga agaagtcgga
     6421 tcagctaatt tctttggaat tacagctgat aatgaatttg taacaccatt gagtccatct
     6481 atcttgccat ctattaccaa gtattccttg ctttatttgg cagaacatcg cttgggatta
     6541 actcctattg agggtgatgt tccaattgat aatcttgacc gttttgtaga ggcaggtgcc
     6601 tgtggtacag cagcggttat ttctccaatt ggaggtattc aacatggtga tgatttccat
     6661 gtattctata gtgaaacaga agtaggtcct gtgacgcgta aattatataa tgaattgacg
     6721 ggtattcagt ttggcgatat tgaagcgcca gaaggttgga ttgtaaaagt agattaaaat
     6781 aaaccaaagg agatttttta tgaaatagaa aaagtggctc ttaacagcag gagtggtcct
     6841 gagcacgtca gctattttag tggcttgtgg aaaaactgat aaagaagcag atgcaccgac
     6901 aacattttct tatgtctatg cagtagatcc agcatcattg ggctacagta tagcgactcg
     6961 aacatcgagg acagacgtta ttggaaatgt tattgatggt ttgatggaaa atgataaata
     7021 cggcaatgtt gctccttctc aaaaagacta tgatttgaac agtacaggat gggctccaag
     7081 ctatcaagat ccagcgtctt acttgaatat tatggatcca aaatctggtt ctgccatgaa
     7141 acaccttggc attacgaaag gaaaagataa ggatgttgta gctaaacctg gtttggataa
     7201 atataagaaa ttgttagaag atgctgtttc tgagaccact gacctagaga agagatatga
     7261 aaaatatgcc aaagctcaag cttggtcgac agatagttca ttattgatgc caacagcttc
     7321 atctggtggt tctccagttg taagtaacgt agtaccattc tcaaaaccat actcacaagt
     7381 tggtattaag ggggaaccat atatctttaa aggaatgaaa ttgcaaaaag atattgttac
     7441 aacaaaagaa tataacgagg tttttaaaaa atggcaaaaa gaaaaattgg aatccaatag
     7501 caaataccaa aaagaactag aaaaatacat taaataagga atggtattga tcttgataaa
     7561 atttttaaaa tactgtcatt ttgaatataa aggagtttga tatggagtgg attagattaa
     7621 taggaatagc aatcattgtt gtgggtttta ttttaaaatt tgatacaatt gcaacagtag
     7681 tcttagctgg tttggttaca gctttagttt caggtgtttc tctcgttgaa tttttggaga
     7741 ttttgggaaa agaatttagc aatcagcgag tgctcacgat ttttatggtt accttgcctc
     7801 ttgtggggct gtcagaaacc tttggactca agcaacgatc aatcgatttg attcgaaaga
     7861 ttaaaggtct gacagttgga aacttctata cagtttattt ctttattcga gagttagctg
     7921 gtttcttttc aattcgtcta ggaggacacc ctcagtttgt cagacctttg gttcaaccta
     7981 tgggagaagc agctgcagag tctcaattag gtagaaagtt aacagaggtt gaagatgaga
     8041 caataaaagc gcgtgcggct gcgaatgaaa attttggaaa tttctttgct caaaatacgt
     8101 ttgtaggtgc tgggggagtc ctcttgatag ggggaacatt agatcagtta ggctatgaaa
     8161 gcaattatgc agggattgct tctacatcta ttattgttgc tgttataaca cttattgtag
     8221 tggggattta caattattta tttgataaaa aattgatatc aaaaaagact aggggaggag
     8281 aacaaaaatg acagagttgg caaagcaact attagagttg acctatattg tgattggttg
     8341 tcaatttctc catacagcct attgtagtta taaagataaa acaaacccag ttcgacttgg
     8401 gacatctgca ttttggactc tattgtctat tacgtttata ggtggttcct atatgccaaa
     8461 tatgagtatt ggtattattg taatcctatt atcgctgtta acattgttta agcaagtccg
     8521 tatcggaacc ttgccatcct tagatgaaat gaaagccaat attgaatcta acaggttgaa
     8581 aaataaaatt tttattccag ttatgctgat ggcaatactt gcgttggtct tagcgcaaat
     8641 gattccagaa tttagcaaga tttcgattag ccttgccgcc ttgtttgcta caatttctgt
     8701 tcttgtgatt accaatagtc accctaagag tctgttatca gaaaataatc gaatgactca
     8761 gcaagtttca acaagtggga ttgttcctca attattaggg gctttggggg ctatttttac
     8821 tgtagcaggt gttggtgatg ttatctctca tctgattagc ggtattgttc cttcagatag
     8881 tcgctttata ggagttttgg cttatgttct tggaatggtt ctattcacaa tgattatggg
     8941 aaatgctttt gcagcattca ccgttattac agcaggtgtt ggagttccct ttgtatttgc
     9001 tctgggagct aatccaattg tggctggtgc tcttgccatg acagcaggtt attgtgggac
     9061 cttattgacc ccaatggctg ctaattttaa cgctctacca gcagcattga tggatatgaa
     9121 agatcagaat ggcgttataa aggctcaagc aggtgttgct ctagtaatga ttgttattca
     9181 catattctta atgtactttc tcgcatttta gtaaaggaaa ataaaatgaa aatattggtt
     9241 acaggtttta atccttttgg aggtgaaaag attaatccag ctttggaggc tgtaaaatta
     9301 ttaccatctg agattaatgg ggctgaagtt cgctgggtag aaattccaac ggttttttat
     9361 aagtcgtcag aagttttaga ggcagaaata ttacgatatc aaccagatgc tgtactttgt
     9421 attggacaag caggcggcag gaccggctta acacctgaac gagtggctat taatcaagat
     9481 gatgctcgca tacctgataa cgaaggcaat caaccaattg atacaccgat tcgtattgat
     9541 ggagcatcgg cctattttag tagtttacct atcaaagcga tggtacaagc tatcaaaaaa
     9601 cagggacttc cggcagttgt atccaatagt gcaggaacct ttgtttgcaa tcatttgatg
     9661 taccaagctc tttatttagt ggataaaaaa ttccctaata tgagagcagg tttcatgcat
     9721 attccatata tgatggaaca agtagtaaat aagccgaata ctgcaggaat gagtctatgt
     9781 gatattgtaa gaggcataga ggtagctatt gaagctattg tagactacaa agataaggat
     9841 ttgcagttag taggcggtga gactcactga taacctaaaa aggatagtca attatgcttg
     9901 tttactaact attaactatg ctaaatcaat tgaggttgtt tacataaaac tctatatcag
     9961 agaagcctga tatagagttt tttcttgcta gttttaggat tttcttgtaa aatagaaaaa
    10021 gtgaagagag gtatgaaatg agcaagaaag ataaaaaaat cgaaattcaa gtagcggatg
    10081 ccaaagttaa tgttggtaaa gacagttttg aaggttatac attgactatc ggtaaaaaag
    10141 ttatcggaga aattgccgaa ttagacggac aatttgccat tataaagaat gggaatgtcg
    10201 atagttttta taaaaaattg gaaaaagctg tggaaatttt gattgaaaat tataatttag
    10261 caaaataagt cttgtttttt tgaaattttc atgatataat agtccatgtt gattgtagga
    10321 gagatagcga agaggctaaa cgcggcggac tgtaaatccg ctccttcggg ttcgggggtt
    10381 cgaatccctc tctctccatt tcattaatgg ggtatagcca agcggtaagg caagggactt
    10441 tgactccctc atgcgttggt tcgaatccag ctaccccagt tcttaggtaa taatcaagat
    10501 agaaagcaaa atatcttagg gtattttatt tttataattg aaagacgtga atgatatgaa
    10561 catgtccttg cgggtgctta ggaaaaaaat tataagtatg tcaagtttaa gaaaaacttg
    10621 attgttggag gattttttag atgaacgaat ttgaagattt gctaaatagc gttagtcaag
    10681 ttgagactgg tgatgttgtt agtgctgaag tattgacagt tgatgcgact caagctaacg
    10741 ttgcaatctc tggaactggt gttgaaggtg tcttgactct tcgcgaattg acaaacgatc
    10801 gtgatgcaga tatcaatgac tttgttaaag taggagaagt attggatgtt cttgtacttc
    10861 gtcaagtagt tggtaaagat actgatacag ttacatacct tgtatctaaa aaacgccttg
    10921 aagctcgcaa agcatgggac aaacttgttg gtcgcgaaga agaagttgtt actgttaaag
    10981 gaacgcgtgc cgttaaaggt ggactttcag tagaatttga aggtgttcgt ggatttatcc
    11041 cagcttcaat gttggatact cgtttcgtac gtaacgctga gcgttttgta ggtcaagaat
    11101 ttgatactaa aatcaaagaa gttaacgcta aagaaaaccg cttcatcctt tcacgtcgtg
    11161 aagttgttga agcagctact gcagcagctc gcgctgaagt attcggtaaa ttggctgttg
    11221 gtgatgttgt aactggtaaa gttgctcgta tcacaagctt cggcgctttc atcgaccttg
    11281 gtggtgttga cggattggtt cacttgactg aattgtcaca tgaacgtaac gtatcaccaa
    11341 aatcagttgt aactgttggt gaagaaattg aagtgaaaat ccttgatctt aacgaagaag
    11401 aaggacgtgt atcactttca cttaaagcaa cagtaccagg accatgggat ggcgttgagc
    11461 aaaaattggc taaaggtgat gtagtagaag gaacagttaa acgtttgact gacttcggtg
    11521 catttgttga agtattgcca ggtatcgatg gacttgttca cgtatcacaa atttcacaca
    11581 aacggattga aaatccaaaa gaagctctta aagttggtca agaagttcaa gttaaagttc
    11641 ttgaagttaa cgcagatgca gaacgcgtgt cactttctat taaagctctt gaagaacgtc
    11701 cagtccaaga agaaggacaa aaagaagaaa aacgtgctgc tcgtccacgt cgtccaagac
    11761 gtcaagaaaa gcgtgatttc gaacttccag aaacacaaac aggattttca atggctgatt
    11821 tgtttggtga tatcgaactt taatcaaatt gaaaattcac aaaatccttt gtttactaaa
    11881 caagggattt ttcttttgtc tctgctcctt ttttgatata atagttctat gttaaaatca
    11941 gaaaaacaat cacgttatca aatgttaaat gaagaattgt ccttcctatt ggaaggcgaa
    12001 accaatgttt tggctaatct ttccaacgcc agtgctctca taaaatcacg ttttcctaat
    12061 accgtatttg caggctttta tttgttcgat ggaaaggaat tggttttagg tcccttccaa
    12121 ggaggtgttt cctgcatccg tattgcacta ggcaagggtg tttgtggtga ggcagctcac
    12181 tttcaggaaa ctgttattgt tggagatgtg acgacctatc tcaactatat ttcttgtgat
    12241 agtctagcta aaagtgaaat tgtggtgccg atgatgaaga atggtcagtt acttggagtt
    12301 ctggatctgg attcttcaga gattgaggat tacgatgcta tggatcgaga ttatttggaa
    12361 caatttgtcg ctattttgct tgaaaagaca acatgggact ttacgatgtt tgaggaaaaa
    12421 tcttaatgta tcaagcactt tatcgaaaat atagaagtca aaacttctcc cagttagttg
    12481 gtcaagaagt tgtggctaag actcttaaac aagcggtgga gcaagagaaa ataagtcacg
    12541 cttatctttt ttctggtcct cgtggaacgg gaaaaaccag tgttgctaaa atctttgcca
    12601 aggctatgaa ctgtcccaat caagtgggtg gcgaaccttg caataactgc tatatttgtc
    12661 aagcagtgac ggacggtagt ttagaagatg tcattgaaat ggatgcagct tctaataatg
    12721 gggtagatga aattcgcgaa attcgtgata aatctaccta tgcgcctagc cttgctcgtt
    12781 ataaggttta tatcatagat gaggttcaca tgctgtctac aggggctttt aatgccctcc
    12841 taaagacgct ggaagaacca acacagaatg tagtctttat tttggccact actgaattgc
    12901 acaagattcc tgctactatt ctatcccgtg tgcaacgttt tgagtttaaa tcaattaaga
    12961 cacaggatat tcaggaacat attcactata tcttagaaaa agaaaatatc agttctgaac
    13021 cagaggctgt ggaaatcatt gccagacggg cggaaggtgg aatgcgggac gccttgtcta
    13081 ttttggatca agccctgagt ttgacacagg gaaatgagct gacgactgct atctctgaag
    13141 aaattactgg caccattagc ctatcagcct tggatgatta tgtggcggcc ttgtctcaac
    13201 aggatgttcc caaagctttg tcttgcttga atcttctttt tgacaatggt aagagcatga
    13261 ctcgttttgt gaccgatctt ttgcactatt taagagactt gttaattgtt caaacagggg
    13321 gagaaaatac tcatcatagt tcagtctttg tagaaaattt ggcacttcct caaaaaaata
    13381 tgtttgaaat gattcgctta gcaacagtga gtttagcaga tattaagtct agtttgcagc
    13441 ccaagattta tgctgaaatg atgaccgtcc gtttggcgga aatcaagccc gaaccagctc
    13501 tatcaggagc ggttgaaaat gaaattgcta cgctgagaca ggaagttgcc cgtctcaaac
    13561 aagagctttc taatgcaggt gcggttccta aacaagttgc accagctcct agtcgaccag
    13621 ctacgggcaa aacagtctat cgtgtcgatc gcaataaagt gcaatctatc ttacaagagg
    13681 ccgtcgaaaa tcctgattta gcacgtcaaa atctaattcg tttgcagaat gcctggggag
    13741 aggtaattga aagtctaggt gggccggaca aggctctgct agttggttct caaccggttg
    13801 ctgccaatga acaccatgct attcttgctt ttgagtctaa cttcaatgct ggtcaaacta
    13861 tgaaacgaga caatctcaat accatgtttg gtaatatcct cagtcaggcg gcaggttttt
    13921 cacctgagat tttagctatt tccatggagg aatggaaaga agttcgcgca gccttttcag
    13981 ccaaagccaa atcttctcaa actgaaaaag aagtagaaga aagcctgatt ccagaaggat
    14041 ttgaattttt ggctgataaa gtgaaggtag aggaagacta aagaaagatt tcatgataca
    14101 ataagtttat gaataaacaa caatttatta ttatggcgct atttacagct gctgagacct
    14161 attttttcaa tgaagcctgg atgactggcc gctatattat ggcagccttt tgggcaattt
    14221 tactctttag aaatttccga gtcagttatg tgatgggtaa aatcgttgat gtcatcgatc
    14281 agcattttaa taggaaagac tagccctcag cttccagaca aaatcaaagc cttttaggct
    14341 tttttttgtt atactagaaa agtatattta tagaattttt gctctatttc tggggaaatc
    14401 agacgttttt ctagtaagta ctgtaaaagt tttgaaaaag aaaggaacta tcatgtcagt
    14461 aatagagatc aaagatcttc acgttgagat tgaaggaaaa gaaattttaa aaggggttaa
    14521 cctgaccctg aaaacaggag aaattgccgc tatcatggga ccaaatggta caggtaaatc
    14581 gactctttct gccgctatca tgggaaatcc aaactatgaa gtaactaaag gtgaagtttt
    14641 gtttgatggc gtaaacatcc ttgagttgaa agtggatgag cgtgcgcgta tgggactttt
    14701 ccttgctatg caatacccat cagaaatccc tggaattacc aatgctgagt ttcttcgtgc
    14761 cgctatgaat gcgggtaaag aagatgatga gaagatttca gttcgtgagt ttattactaa
    14821 gctagatgaa aaaatggaat tgctcaacat gaaagaagaa atggcagagc gttacctcaa
    14881 cgaaggcttc tctggtggtg agaaaaaacg caatgaaatt cttcaacttt tgatgttgga
    14941 gccaacattt gctcttttgg acgagattga ctcaggtctt gatattgacg ctcttaaagt
    15001 tgtgtctaaa ggtgtcaatg ccatgcgtgg tgaaggtttt ggtgctatga tcatcactca
    15061 ctaccaacgt cttttgaact atatcacacc tgatgtggta cacgtgatga tggaaggtcg
    15121 tgttgtcctt tctggtggtc cagaattggc tgcgcgtttg gaacgtgaag gatacgcaaa
    15181 attagctgaa gaacttggct acgactacaa ggaagaattg taattccctc gtatctttta
    15241 ggagaagtaa atgactagag aaaatattaa acttttttca gaaatgcacg ctgaaccaag
    15301 ctggttggct gatctccgtc aaaaagcttt tgacaagatt gagactttgg aattaccagt
    15361 tattgagcgt gtcaaattcc accgttggaa tctgggtgat ggaacgatta cagaaaatga
    15421 gccatcagca aatgttccag atttcacagc tttagatcat cacttgaagt tggtgcaagt
    15481 aggaactcaa actgttttcg aacaaactcc agttgagtta gctgaacagg gtgttgtctt
    15541 cacagacttt cactcagctt tagaagaaat tccagagctg atcgaagaat tcttcatgtc
    15601 atctgttaag tatgatgatg acaagttggc ggcttaccac acagcttact ttaacagtgg
    15661 tgctgtactc tatattccag ataacgtaga aattacagag ccaattgaag gaattttcta
    15721 ccaagatagc gatagcaatg tgccgtttaa caagcatatt atgattatcg ctggtaaaaa
    15781 ttctaagatt agttatctgg agcgtttaga gtcacgcggt gaaggaagtg acaaagcaac
    15841 tgccaatatc acagtggaag tgattgcacg ttctggtgcg caagtcaagt ttgctgctat
    15901 cgaccgtcta ggtgaaaacg tcactgccta cattagccgt cgtggtaaat taggcaacga
    15961 tgcaagtatt gactgggcta tcggtgtcat gaacgaagga aatgtcgttg ctgattttga
    16021 tagtgacttg attggtaatg gtagccatgc tgacctgaag gttgtagctc tttcaagtgg
    16081 tcgtcaggta caagggattg atactcgtgt aactaactat ggctgcaact ctatcggaaa
    16141 tatcctacaa catggggtta tccttgaaaa agcaactttg actttcaatg gtatcggcca
    16201 catcatcaag ggtgctaagg gagcagatgc ccaacaagag agccgtgttc tcatgctttc
    16261 agaccaagcg cgttcagatg ctaacccaat tcttttgatt gatgaaaatg atgtcactgc
    16321 aggacacgca gcgtctatcg gacaagttga tccagaagac atgtattacc tcatgagtcg
    16381 tggattggat aaggcaactg cagagcgttt ggttgttcgt ggtttccttg gatctgttat
    16441 cgtggagatt ccagtcaagg aagttcgtga tgaaatgatt gcaactatcg aagagaaatt
    16501 gtcaaaacgc taaggggaag cctatgttag atgtagaagc gattcgcaag gattttccaa
    16561 ttttagatca gattgtcaat gatgaacctc tggtctatct ggacaatgct gcgacgacac
    16621 aaaaaccact agtagttctg aaagttatta acagctacta tgagcaggac aatgccaatg
    16681 ttcaccgtgg tgtccatacc ttagcggaac gagcgacagc ttcttatgaa gctgctcgtg
    16741 aaaccattcg taagtttatt aatgcaggct ctacaaagga agttcttttt accagaggaa
    16801 cgacaaccag ccttaactgg gtagcacgct ttgctgagga aattctcact gagggagacc
    16861 aggtcttgat ttcagtaatg gaacaccatt ctaatatcat tccatggcag gaagcttgtc
    16921 gaaagactgg agcagagctt gtctatgtct atcttaaaga cggtgccttg gatatggagg
    16981 atttgcgagc taaattgact gataaggtta aatttgtttc cctagctcat gtctccaatg
    17041 ttcttagtgt ggtcaatccg atcaaggaaa tcactcaatt agcccaccaa gttggggcaa
    17101 tcatggtagt ggatggtgct caatctacac ctcatatgaa gattgatgtc caggacttgg
    17161 atctggactt tttcgccttt tcgggtcaca agatggctgg tccgactggt atcggtgtcc
    17221 tttacggcaa agaaaagtat cttgagcaaa tgtctccagt agaatttggc ggcgagatga
    17281 ttgattttgt ctacgagcaa tccgctagtt ggaaggaatt gccttggaaa tttgaggctg
    17341 gaacgccaaa tatggcagga gctattggac ttgcgactgc agttgattat ctggaaaaga
    17401 ttggtatgga tgccgttgaa gctcatgaac aggaattgat tgcgtacgtc tatccaaaac
    17461 tgcaggcaat tgagggattg accatttacg gttctcagga tttggctcaa cgttcgggtg
    17521 ttattgcctt taacctaggt gatctccatc ctcacgatct tgcgacggct ctggattatg
    17581 aaggagtggc tgttcgtgct ggtcaccatt gtgcgcaacc cttgcttcag tatttggaag
    17641 tcccagcaac agctcgtgca agtttttata tctacaatac caaggcagat tgcgacaaac
    17701 tagtcgatgc cctacaaaag acaaaggagt ttttcaatgg cactttctaa actagatagc
    17761 ctttatatgg cagtggtagc agaccattcg aaaaatccac atcaccaagg gaagttagaa
    17821 gacgctgagc aaatcagtct caacaatccg acttgtgggg atgtcatcaa cctctctgtc
    17881 aagtttgatg cagaggaccg tttggaagat attgcttttc taaattcagg atgcacgatt
    17941 tcaactgctt ctgctagtat gatgacagat gccgttttag gaaaaaccaa acaagaaatt
    18001 ttagaactgg cgactatttt ttctgaaatg gttcaagggc aaaaagatga gcgtcaagac
    18061 caacttggag acgcggcatt cttgtcaggt gttgccaaat tccctcaaag aatcaagtgt
    18121 gcaaccctag cttggaatgc ccttaagaaa acaattgaaa atcaagaaaa acagtaagac
    18181 aagtttcttt tgtcttatga attattagaa atgaagaaag aaaggatact atggctgaag
    18241 aaagagtaga accaaaacca attgaccttg gtgaatataa atttggtttc catgacgatg
    18301 tagagcctgt cttatcgaca ggaaaaggac tcaacgaagg tgttattcgt gaattatctg
    18361 ctgctaaggg tgagcctgag tggatgttgg agttccgttt gaagtcttat gaaaccttca
    18421 aaaaaatgcc catgcaaact tggggagcag acttgtcaga gattgacttt gatgacttaa
    18481 tctactacca aaaaccatct gacaaaccag cccgttcttg ggatgatgta cctgaaaaga
    18541 ttaaagaaac ctttgaacgt atcgggattc cagaagctga acgtgcttat ttagcagggg
    18601 cttctgccca gtacgagtca gaagtggttt accacaacat gaaggaagag ttccaaaaat
    18661 taggtattat ctttacagat acagattccg cactcaagga atacccagac ttatttaaac
    18721 aatactttgc gaagttggta ccgccgacag ataacaagtt ggcagccctc aactcagcag
    18781 tatggtcggg tggaactttt atctacgtgc caaaaggtgt caaggtagat attccacttc
    18841 aaacttattt ccgtatcaat aacgaaaata taggtcagtt cgaacgtacc ttgattatcg
    18901 ttgatgaggg agcaagcgtc cactacgtag aaggatgtac agcaccaaca tattcaagca
    18961 atagcttaca cgctgccatt gtagaaattt ttgctttgga cggagcttat atgcgttata
    19021 caactatcca aaactggtct gataacgtct ataacttggt aacaaagcgt gctaaggctc
    19081 aaaaggatgc cactgttgag tggattgatg gaaacttggg tgccaaaacg actatgaaat
    19141 atccatctgt ttaccttgat ggagaaggag cgcgtggtac catgctctct atcgcctttg
    19201 ctaatgcagg gcaacaccaa gacacgggtg ctaagatgat tcacaatgct ccacatacca
    19261 gctcgtctat tgtgtctaaa tccatcgcta aaggtggagg aaaggttgac taccgtggac
    19321 aagtcacctt taacaagaac tctaagaaat ctgtttccca cattgaatgt gataccatta
    19381 tcatggatga cttgtcagca tcagatacta ttccatttaa tgaaattcac aactcgcaag
    19441 tggctttgga acacgaagcc aaagtatcta agatttcaga agagcaattg tattatctca
    19501 tgagccgtgg attgtcagaa tctgaggcaa ctgaaatgat tgtcatggga tttgtagaac
    19561 cctttacaaa agaacttcca atggaatacg cagttgagct gaaccgcttg attagctatg
    19621 aaatggaggg atcagttgga taaaatttga ttttatactc ttcgaaaatc tcttcaaacc
    19681 acgtcagcat cgccttaccg tatgtatggt tactgacttc gtcagtttca tctacaacct
    19741 caaaacagtg ttttgagcaa cctgcggcta gcttcctagt ttgttctttg attttgaaga
    19801 tgaacttgta tcaaaaaatc gcggtttaaa atcgcgattt tttataattt ctcgttaaca
    19861 aagcggacaa actgattcca ccaaactttt aagaagaagg ctttttcaat tttcttgtct
    19921 gctaccattt cgaaactagg gcgctctgtg gtgatgtaac cttgaccaat caagtccttg
    19981 tcttcataag tcaaatggcc aaccactgtt ccagcttcaa gtggtgctgg gattgctttg
    20041 gaatcaggtg tgaattgaac agattgggaa gattgattcc caacacgttc gattagatag
    20101 atatcctctg gagccactgc agttactgta tcttcttttc catcttgtac aggggctttg
    20161 ctatcttgat aggcatcgcc ttgttgaacg attttgcgaa gtgtaaatgt agaagaaata
    20221 taatccatta gggaagatgt agctgtaaat cgagcgtaag gattattgtc ttgatgatct
    20281 gcatttaaaa caactgtgat gactctcatg cctttttcga cagtagtacc aacaaaagac
    20341 tctccagcct tatctgttgt tcctgttttt agcccatcaa aaccaccacg gtaagcaggc
    20401 ataccttcta acatgtagtt ggttgaagtg attgtcatcc cagcaaaagt agaagaaggt
    20461 tttttggtga tttctaagac ttgtgggtat tttttgatga gattgcgagc aacgatagcg
    20521 acatcataag cactaagctt attttcctca tcttttttag aacctgggta aatgttatcc
    20581 cctagagttt cattgttaag acctgtcgta ttgacaacag tggcatcctg aattccccat
    20641 tccaagagtt ttgcccgcat catatcgacg aaatcttttt ctgagccagc aattttctca
    20701 gctagggcaa tagcggcgct gttggcacta gataccagag ttgcttcaag caactcttcg
    20761 acagtataat tacgggcctc cataggaata ttactggctt cagaatttgt cgtcaattga
    20821 taaggataat cagaaatatc tacaggagtg gagagggtaa tacttccgtt ttccaaagct
    20881 tcatagacca gataaacagt aatcaatttt gttatggaag caatttcgac aggttgcgtt
    20941 gcatccttct catagagaat tttaccagta tttgcctcaa cagcaatcgc atgtttagcg
    21001 gcaatggtaa aatcttgagc aacagcagta gaagcacccc ctaaaagaga gacagttaac
    21061 aaagttaaaa atattttttt catagtagtc ttattctatc ataaagaaaa aaaatattct
    21121 tgctttaata attcatctgt taagcttttt gaaaatatgg taaaataaag tgagggaggt
    21181 aactcatgtt tcgtagaaat aaattatttt tttggaccac agaaatttta ctcttaacca
    21241 tcatctttta cctatggaga cagatggggt ctttgattaa cccttttgtt agcgtgctta
    21301 atacaattat gattccattt ttattagggg gctttcttta ttatttgaca aaccctattg
    21361 ttattttctt aaataaagtc tgtaaactca atcgtttgct tggtatttta attaccttgt
    21421 gtactttggt ctggggaatg gtcataggtg ttgtctatct cttacctatt ttgattaatc
    21481 agttatctag tttgattata tctagtcaaa ctatttatag tcgagtacaa gacttaatca
    21541 tagacttatc taattatcct gcgctccaga atttggatgt agaagctaca attcagcagt
    21601 taaacttatc ctatgttgat attcttcaaa atatcctaaa tagcgtatca aatagtgtgg
    21661 ggagcgtctt gtcagctctt atcagtactg ttttgatttt gattatgact ccagtttttt
    21721 tggtttattt cttattagat ggacataaat tcttgcccat gcttgaaaga acgattctaa
    21781 agagggatcg cttgcatatt gcaggcttat taaagaattt aaatgcgacg attgctcgct
    21841 atattagtgg agtttcgatt gacgcaatca ttataggttg tttggcttat attggctata
    21901 gtattattgg tttaaaatat gctttagttt ttgccatttt ttctggtgta gccaatttaa
    21961 ttccttatgt ggggccaagt attggtttga ttcctatgat catcgcaaat atattcactg
    22021 atccccatag actgctgatt gcagtgattt atatgcttgt tgttcagcag gtagatggca
    22081 atatcttata tcctcgaatc gtaggaagtg ttatgaaggt tcatccaatc acgattttag
    22141 ttttactttt gttgtcaagc aatatctatg gtgtagttgg aatgattgtc gcagtgccaa
    22201 cctattctat cttgaaagaa atttctaagt tcttatcccg tttgtatgaa aatcataaaa
    22261 taatgaaaga acgagaaaga gaattagcta agtaaaagtc aggagaaccc tgatttttct
    22321 ttactggaag tggcctttag attagaagac tgaaaataag ttaaagtctt aaactaattt
    22381 tcacagctaa gaatagtaga agttaatctg ataaaaatcg aaaaaaccag tggaattctg
    22441 tgtcagggta agttccactg gttttcatag tctattaaag ttcgaatgaa acctatttat
    22501 agtagattga aactagaata gtacacctct aattctaaaa cattgttaga aatcgatttg
    22561 actgtcctga tctattcgtt ctattcttat ttcattttac tatattttgg tgcaataagt
    22621 gaaaagtagt ccgaataata taaggattga ttttatagtt tttaaactca aatgaattga
    22681 aataaagaga gtacgaaaat tctcatctga aagtatttta gaataattct cttcgtgaat
    22741 ttcttcaaaa cagatagctt catcttaggt atgtgatttc tttttgcatt tttgagttag
    22801 ataaggtata atggttttat tgtcttttgg ggtcgttacg gattcgacag gcattatgag
    22861 gcatattttg cgactcgtgt ggcgacgtaa acgctcagtt aaatataact gcaaaaaata
    22921 acacttctta cgctctagct gcctaaaaac cagcaggcgt gacccgattt ggattgctcg
    22981 tgttcaatga caggtcttat tattagcgag atacgattaa gccttgtcta gcggtttgat
    23041 aagagattga tagactcgca gtttctagac ttgagttatg tgtcgagggg ctgttaaaat
    23101 aatacataac ctatggttgt agacaaatat gttggcaggt gtttggacgt gggttcgact
    23161 cccaccggct ccattattcc tttgcattct tttgcattcc ttggtaaaac gttgttaaat
    23221 caacgttttt tatttttatc tttggtattc ctttgcattc ttttgctaaa aagggagtca
    23281 caaacagacc ctattttaaa aaaaggatag aaaaaaggat acaacatttg tcgcatccta
    23341 aaaataatct tttttcgacg gaagacatgg gattcgaacc cacgcacgct attacacgcc
    23401 taccgcgttt ccaacacggc ctcttaagcc tcttgagtaa tcttccaata cttactcaaa
    23461 tagtctacca taaaggctct tatcttgcaa taaaaattct agaaataaga aaaaatgata
    23521 gattttgaaa gaaaatgata aaaaatgctt gacttcgaaa gaaagtatga tagaatgaat
    23581 agtgtaaacg ataacaggag gtgattcagt gttaaaaaca gaacgtaaac aactaatttt
    23641 agaggagtta aatcagcatc atgtagtttc tctagaaaaa ttagttagtt tgctagaaac
    23701 gtcagaatca acggttcgaa gagacttgga tgagttggaa gcggaaaaca agcttcgtcg
    23761 tgtgcatggt ggagcagaac tcccccactc cttacaggaa gaagaaacca ttcaagaaaa
    23821 atctgtcaaa aaccttcaag aaaagaaatt gctggctcag aaagcagcct ctctcattaa
    23881 agaaaaagat gtcatcttta tcgatgctgg aacaacaact gcttttttga ttcatgaatt
    23941 ggtcaataag aatgttacag ttgtgaccaa ctccattcac catgccgctc agttggttga
    24001 aaagcagatt ccaactgtca tggttggagg aaacgtcaag acggcgacag atgctagtat
    24061 cgggggcgtt gctcttaacc agattaacca attgcacttt gaccgtgcct ttatcggaat
    24121 gaatggtgtt gacgatggct attatacgac tcctgatatg gaggagggag ctgtgaaaag
    24181 agctattttg gagaatgcca agcagaccta cgtcttggtg gattcgtcaa aaattggaca
    24241 aacttgcttt gccaaggtag ccccactcaa acgcgctatc gttatcacta gtcaagggca
    24301 tgagctcttg caggttatta aggagaaaac ggaggtaata gaagtatgat ttatacagtc
    24361 acactcaatc catccattga ctatatcgtt cgtttggacc aagtcaaagt tggtagtgta
    24421 aatcgtatgg acagtgatga taagtttgct ggtgggaaag gaatcaatgt cagccgtgtc
    24481 ttgaaacgtt tgaatatatc aaatacagcg acgggattta tcggtggctt tactggtaaa
    24541 tttatcacag atactttagc agaggaagaa atcgagacac gttttgtcca ggtggcagaa
    24601 gatactcgta tcaatgttaa aatcaaagca gaccaagaaa cagaaatcaa cggaacgggt
    24661 ccaactgttg aaccggttaa gctagaagaa ttgaaagcta ttttatctag tctgacagca
    24721 gaagatacag ttgtctttgc aggttcaagt gctaaaaatc taggcaatgt tatctataag
    24781 gatttgattt ccttgacgcg ccagactggt gcgcaagtgg tctgtgactt tgaaggacag
    24841 accttaattg atagtttgga ctaccagcct cttcttgtaa aaccaaataa tcatgaactt
    24901 ggagcgattt ttggggttaa actcgaaagt ttagatgaaa ttgagaaata cgctcgtgag
    24961 ttactggcta agggtgctca aaatgttatt atctctatgg ctggtgatgg tgcccttctt
    25021 gtcacatctg agggagctta cttcgctaaa ccaatcaaag gaacagtcaa aaattcagtt
    25081 ggagctggtg attctatggt tgctggattc acaggtgaat ttgtcaaatc aaaagacgca
    25141 gtagaagcct tcaaatgggg agtggcttgc ggaacggcaa ctaccttctc agatgacttg
    25201 gcaacggcgg aatttattaa agaaacatat ggaaaagttg aggtagaaaa acgatgaaaa
    25261 ttcaagattt attgagaaaa gatgtcatgt tgctggattt gcaggcaact gaaaagactg
    25321 ctgtcattga agagatgatt aaaagcctaa caggccacgg ttatgtgaca gattttgaaa
    25381 cctttaaaga aggcattttg gcgcgtgaag ctttaacttc tactggtttg ggtgatggaa
    25441 tcgctatgcc tcacagcaaa aacgctgctg tcaaagaagc gacagttctc tttgctaagt
    25501 caaacaaggg tgttgactat gagagtttgg atgggcaggc aactgacctc ttcttcatga
    25561 ttgcagctcc agaaggtgcc aatgatactc acttggcagc attgacagaa ttgtctcaat
    25621 acttgatgaa agacggtttt gcagacaaac ttcgtcaagc aacatctgca gaccaagtta
    25681 tcgaactttt tgaccaagct tcagaaaaaa ctgaggaact tgttcaagca cctgctaatg
    25741 actctggtga ctttatcgta gctgttacag cttgtacaac aggtattgcc cacacttaca
    25801 tggcccaaga agcccttcaa aaagtagctg ctgaaatggg ggttggtatc aaggtcgaaa
    25861 ccaacggtgc tagcggtgtt ggaaatcaac taactgcaga agatatccgt aaggctaaag
    25921 ctattatcat tgcagcagac aaggccgttg aaatggatcg tttcgatggc aaacctttga
    25981 tcaatcgtcc agttgctgac ggtatccgta agacagaaga attgatcaac ttggctattt
    26041 caggagatgc ggaagtctac cgtgccgcta atggtgccaa agtcgcaaca gcctctaacg
    26101 aaaaacaaag ccttggtggt gccttctaca aacacttgat gagtggtgta tctcaaatgt
    26161 taccattcgt tatcggtggt ggtatcatga ttgcccttgc cttcttgatt gacggtgctt
    26221 tgagtgttcc aaatgaaaac cttggcaatc ttggttctta ccatgagtta gcttctatgt
    26281 tcatgaaaat tggtggagct gcctttggtt tgatgcttcc agtctttgcg ggttatgttg
    26341 cctactctat tgctgaaaaa ccaggtttgg tagcaggttt cgtggctggt gctattgcca
    26401 aagaaggttt tgcctttggt aaaattcctt atgccgcagg tggtgaagca acttcaactc
    26461 ttgcaggtgt ctcatctggt ttcctaggtg cccttgttgg tggatttatc gcaggtgcct
    26521 tggttcttgc catcaagaaa tacgttaaag ttcctcgttc actcgaaggt gctaaatcaa
    26581 tccttctatt gccacttctt ggaacaatct tgacaggatt tgttatgcta gctgtgaata
    26641 tcccaatggc agcaatcaac actgctatga atgacttcct aggcggtctt ggaggaggtt
    26701 cagctgtcct tcttggtatc gtccttggtg gaatgatggc tgttgacatg ggtggaccag
    26761 ttaataaagc agcttatgtc tttggtacag gtacgcttgc agcaactgtt tcttcaggtg
    26821 gttctgtagc catggcagca gttatggctg gaggaatggt gccaccactt gcaatctttg
    26881 tcgcaactct tcttttcaaa gataaattta ctaaggaaga acgtaactct ggtttgacaa
    26941 acatcatcat gggcttgtca tttatcactg agggagcgat tccatttggt gccgctgacc
    27001 cagctcgtgc gattccaagc ttcatccttg gttcagcagt agcaggtgga ctcgttggtc
    27061 ttactggtat caaactcatg gcgccacacg gaggaatctt cgttatcgcc cttacttcaa
    27121 atgctctcct ttacctcgtt tctgtcttgg taggagcaat cgtaagtggt gtggtttatg
    27181 gttacctacg caaaccacaa gcataaaaaa tagaaaaatg aaaagattgg accgtttggt
    27241 gcagtctttt tctcttcccg aaatgcctgt gaaatatggt ataatagaag aatggcaaac
    27301 aagaatacaa gtacaacaag acggagaccg tctaaagcag aactggaaag aaaagaagcg
    27361 attcaacgaa tgttgatttc gttaggaatt gcgattttat tgattttcgc agccttcaaa
    27421 ttaggggctg caggtataac cctttataat ttaattcgct tgctagtggg tagcctagct
    27481 tatctggcga tattcggcct attaatctat ctcttctttt tcaagtggat acgaaaacag
    27541 gaaggactct tatctggctt tttcaccata tttgctggct tactcttgat ttttgaggcc
    27601 tacttggttt ggaaatatgg tttggacaag tccattctaa aagggaccat ggctcaggtt
    27661 gtgacagatc tgactggttt tcgaacgact agctttgctg gagggggctt gatcggggtc
    27721 gctctttata ttccaacagc ctttctcttt tcaaatatcg gaacttactt tattggttct
    27781 atcttgattt tagtgggttc tctcctagtc agcccttggt ctgtttacga tattgctgaa
    27841 tttttcagta gaggctttgc caaatggtgg gaagggcacg agcgtcgaaa agaggaacgc
    27901 tttgtcaaac aagaagaaaa agctcgccaa aaggctgaga aagaggctag attagaacaa
    27961 gaagagactg aaaaagcctt actcgatttg cctcctgctg atatggaaac gggtgaaatt
    28021 ctgacagagg aagctgttca aaatcttcca cctattccag aagaaaagtg ggtggaacca
    28081 gaaatcatcc tgcctcaagc tgaacttaaa ttccctgaac aggaagatga ctcagatgac
    28141 caagatgttc aggtcgattt ttcagccaaa gaagcccttg aatacaaact tccaagctta
    28201 caactctttg caccagataa accaaaagat cagtctaaag agaagaaaat tgtcagagaa
    28261 aatatcaaaa tcttagaagc aacctttgct agctttggta ttaaggtaac agttgaacgg
    28321 gccgaaattg ggccatcagt gaccaagtat gaagtcaagc cggctgttgg tgtaagggtc
    28381 aaccgcattt ccaatctatc agatgacctc gctctagcct tggctgccaa agatgtccgg
    28441 attgaagcac caatccctgg gaaatcccta atcggaattg aagtgcccaa ctccgatatt
    28501 gccactgtat ctttccgaga actatgggaa caatcgcaaa cgaaagcaga aaatttcttg
    28561 gaaattcctt tagggaaggc tgttaatgga accgcaagag cttttgacct ttctaaaatg
    28621 ccccacttgc tagttgcagg ttcaacgggt tcagggaagt cagtagcagt taacggcatt
    28681 attgctagca ttctcatgaa ggcgagacca gatcaagtta aatttatgat ggtcgatccc
    28741 aagatggttg agttatctgt ttacaatgat attccccacc tcttgattcc agtcgtgacc
    28801 aatccacgca aagccagcaa ggctctgcaa aaggttgtgg atgaaatgga aaaccgttat
    28861 gaactctttg ccaaggtggg agttcggaat attgcaggtt ttaatgccaa ggtagaagag
    28921 ttcaattccc agtctgagta caagcaaatt ccgctaccat tcattgtcgt gattgtggat
    28981 gagttggctg acctcatgat ggtggccagc aaggaagtgg aagatgctat catccgtctt
    29041 gggcagaagg cgcgtgctgc aggtatccac atgattcttg caactcagcg tccatctgtt
    29101 gatgtcatct ctggtttgat taaggccaat gttccatctc gtgtagcatt tgcggtttca
    29161 tcaggaacag actcccgtac gattttggat gaaaatggag cagaaaaact tcttggtcga
    29221 ggagacatgc tctttaaacc gattaatgaa aatcatccag ttcgtctcca aggctccttt
    29281 atctcggatg acgatgttga gcgcattgtg aacttcatca agactcaggc agatgcagac
    29341 tacgatgaga gttttgatcc aggtgaggtt tctgaaaatg aaggagaatt ttcggatgga
    29401 gatgctggtg gtgatccgct ttttgaagaa gctaagtctt tggttatcga aacacagaaa
    29461 gccagtgcat ccatgattca gcgtcgtttg tcagttggat ttaaccgtgc gacccgtctc
    29521 atggaagagc ttgagatggc aggtgtcatt ggtccagctg aaggtaccaa gccaagaaaa
    29581 gtattgcagc aataaaaaat agcttctttc caaggttgga aggagctatt ttactggcta
    29641 ttgattactt ttatactctt cgcaaatctc ttcaaaccac gtcagctttg ccttgccgta
    29701 tatatgtgac tgacttcgtc agtcttatct acaacctcaa aacagtgttt tgagcagcct
    29761 acggctagct tcctagtttg ttctttgatt ttcattgagt attattttct gaagttggag
    29821 tattgggctg tttttcattt tcagtggcag gtttacttgt agcaggtgta gtagagtcct
    29881 gagttgctgt tttctgatct tcttttttct cttccttgac gctagatttt ggcgtttctt
    29941 cttgttgtgt tttttcttga gaagtgttat tttccttatt tggagtagta ttttcctgag
    30001 gggattcttt ttgaatttct ttgacaaagg ttgtcgtctg gcttgtcgta ggttcttttt
    30061 tagtattttt attattatta tccaaggcgt tcatgatcgt gatacccgct gtaatcaaac
    30121 caaggatact accgatagta gtaatggttt tttgaaaaac agtaaagcct tttttgatat
    30181 gttctgtttt cggttttgaa gttctacgat agttagacat acattctctc ctttgattat
    30241 gatttattat actctgtttc tttaaaaaaa tcttaaaaaa agaggagatt tttcta
//
LOCUS       DBERMR010000026        29562 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.026, whole genome shotgun sequence.
ACCESSION   DBERMR010000026 DBERMR010000000
VERSION     DBERMR010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 29562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29562
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.026"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..92
                     /locus_tag="ACL8A1_001224"
     CDS             <1..92
                     /locus_tag="ACL8A1_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGS1201995.1"
                     /translation="DYIDYYNNKKIKIKLKGLSPVQYRTKSFG"
     gene            complement(220..1242)
                     /gene="cbpF"
                     /locus_tag="ACL8A1_001225"
     CDS             complement(220..1242)
                     /gene="cbpF"
                     /locus_tag="ACL8A1_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpF"
                     /protein_id="HGS1201996.1"
                     /translation="MKLLKKMMQVALATFFFGLLGTSTVFADDSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDPLSGEMVVGWQYIPAPHKGVTIGPSPRIEIALRPDWF
                     YFGQDGVLQEFVGKQVLEAKTATNTNKHHGEEYDSQAEKRVYYFEDQRSYHTLKTGWI
                     YEEGHWYYLQKDGGFDSRINRLTVGELARGWVKDYPLTYDEEKLKAAPWYYLDPVTGI
                     MQTGWQYLGNKWYYLHSSGAMATGWYKEGSTWYYLDTENGDMRTGWQYLGNKWYYLRS
                     SGAMATGWYQEGSTWYYLNASNGDMKTGWFQVNGNWYYAYDSGALAVNTTVGGYYLNY
                     NGEWVK"
     gene            complement(1261..2082)
                     /gene="cbpG"
                     /locus_tag="ACL8A1_001226"
     CDS             complement(1261..2082)
                     /gene="cbpG"
                     /locus_tag="ACL8A1_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpG"
                     /protein_id="HGS1201997.1"
                     /translation="MNVEGRGSANFIKDNVLITAAHNYYRHDYGKEADDIYVLLAVSP
                     SQEPFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEKPIGAKLGTLGLPTSQKNLT
                     GITVTITGYPSYNFKIYQMYTDKKQVLSDDGMFLDYQVDTLERSSGSAVYDASHRVVG
                     VHTLGDGANQINSAVKLNERNLSFIYSVLKGYSLEGWKKINGSWYYYRQHDKQTGWQE
                     INDTWYYLDSSGKMLTDWQKVNGNWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            complement(2163..2402)
                     /locus_tag="ACL8A1_001227"
     CDS             complement(2163..2402)
                     /locus_tag="ACL8A1_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1201998.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPGSNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(2552..3321)
                     /locus_tag="ACL8A1_001228"
                     /pseudo
     CDS             complement(2552..3321)
                     /locus_tag="ACL8A1_001228"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(3322..3954)
                     /locus_tag="ACL8A1_001229"
     CDS             complement(3322..3954)
                     /locus_tag="ACL8A1_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGS1201999.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQDEF"
     gene            complement(3968..4963)
                     /locus_tag="ACL8A1_001230"
     CDS             complement(3968..4963)
                     /locus_tag="ACL8A1_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGS1202000.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSITCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQIQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFRLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(4960..5658)
                     /gene="liaF"
                     /locus_tag="ACL8A1_001231"
     CDS             complement(4960..5658)
                     /gene="liaF"
                     /locus_tag="ACL8A1_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HGS1202001.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(5736..6746)
                     /gene="fni"
                     /locus_tag="ACL8A1_001232"
     CDS             complement(5736..6746)
                     /gene="fni"
                     /locus_tag="ACL8A1_001232"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HGS1202002.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(6730..7737)
                     /locus_tag="ACL8A1_001233"
     CDS             complement(6730..7737)
                     /locus_tag="ACL8A1_001233"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HGS1202003.1"
                     /translation="MIAVKTCGKLYLAGEYAILEPGRLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLEICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYDVSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKAAAWLEEENLATVLERDWGFFISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLTSSKETVVSLVEALEQGKSEKIIEQVAVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(7724..8677)
                     /gene="mvaD"
                     /locus_tag="ACL8A1_001234"
     CDS             complement(7724..8677)
                     /gene="mvaD"
                     /locus_tag="ACL8A1_001234"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HGS1202004.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMAFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(8659..9537)
                     /gene="mvk"
                     /locus_tag="ACL8A1_001235"
     CDS             complement(8659..9537)
                     /gene="mvk"
                     /locus_tag="ACL8A1_001235"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HGS1202005.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVS
                     AESPWRLYEEDTLSMAVYASLEYLDITEACVRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(9659..9988)
                     /locus_tag="ACL8A1_001236"
     CDS             complement(9659..9988)
                     /locus_tag="ACL8A1_001236"
                     /inference="COORDINATES: protein motif:HMM:NF024736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGS1202006.1"
                     /translation="MTRKVYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMLPHDFPLYLMVWSFFRRSMTTGWFQVNGRWYYAYSSGALAVNTTVDGYFVN
                     YNGEWVQ"
     gene            10599..11474
                     /locus_tag="ACL8A1_001237"
     CDS             10599..11474
                     /locus_tag="ACL8A1_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HGS1202007.1"
                     /translation="MYPPELDSKILSIIKKAVTIRTTHTYEYQYSLLFGDAGYLWLLL
                     HLFSISKNQYYLQLANVTAKKLIENYDTLEEIDFALGKSGVLLSLIKYYQFTNDNTLK
                     IFIHNSIGEIYHYFLQRDTAKESILDYSFAHGYCGIAYALFAYSKVLEPSMFYNDLHT
                     FHTELKKLLEKVTSNTENLGNLQLSWCKGISGIILYLCMYDCDGNKDIISKYQEFVFN
                     HHLKMMTGYCHGITSLLQTTVYNQNKLLMKKIQQVILACSERDDHGLLMFQGDSGKAD
                     LFDFGIGSMGYIGVY"
     gene            11517..12749
                     /locus_tag="ACL8A1_001238"
     CDS             11517..12749
                     /locus_tag="ACL8A1_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGS1202008.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALWIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLLALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLTLITSILCQAYIVIFMTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(12867..13865)
                     /gene="cbpJ"
                     /locus_tag="ACL8A1_001239"
     CDS             complement(12867..13865)
                     /gene="cbpJ"
                     /locus_tag="ACL8A1_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpJ"
                     /protein_id="HGS1202009.1"
                     /translation="MKLLKKTMQAGLTVIFFGLLATNTVFADNSEVWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDSLSGEMVVGWQYIPFPSKGSTIGPYPNGIRLEGFPKS
                     EWYYFDKNGVLQEFVGWKTLEIKTKDSVGRKYGEKREDSEDKEEKRYYTNYYFNQNHS
                     LETGWLYDQSNWYYLAKTEINGENYLGGERRAGWINDDSTWYYLDPTTGIMQTGWQYL
                     GNKWYYLRSSGAMATGWYQEGTTWYYLDQPNGDMKTGWQNLGNKWYYLRSSGAMVTGW
                     VKDDSTWYYLNAGNGDMKTGWFQVNGRWYYAYSSGALAVNTTVDGYSVNYNGEWVR"
     gene            complement(13884..14906)
                     /gene="cbpC"
                     /locus_tag="ACL8A1_001240"
     CDS             complement(13884..14906)
                     /gene="cbpC"
                     /locus_tag="ACL8A1_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HGS1202010.1"
                     /translation="MKLLKKMMPIALAVFFFGLLATSTVFADTMGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIHGTGYRDDLFDNRPVFEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKKTYYFDNNYAVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDASGKML
                     TDWQKVNGKWYYFGSSGSMATGWKYVRGKWYYLDNKNGDMKTGWQYLGNKWYYLRSSG
                     AMVTGWYQDGSTWYYLDAENGDMKIGWTKVNGKWYYLNSSGAMVTGSQTIDGKVYNFA
                     SSGEWI"
     gene            complement(15005..15694)
                     /locus_tag="ACL8A1_001241"
     CDS             complement(15005..15694)
                     /locus_tag="ACL8A1_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGS1202011.1"
                     /translation="MGKRILLFEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVFIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            complement(15706..17130)
                     /gene="gndA"
                     /locus_tag="ACL8A1_001242"
     CDS             complement(15706..17130)
                     /gene="gndA"
                     /locus_tag="ACL8A1_001242"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HGS1202012.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVSDVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEDQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            complement(17206..18600)
                     /gene="mapZ"
                     /locus_tag="ACL8A1_001243"
     CDS             complement(17206..18600)
                     /gene="mapZ"
                     /locus_tag="ACL8A1_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HGS1202013.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVNRSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKSAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            complement(18613..19770)
                     /locus_tag="ACL8A1_001244"
     CDS             complement(18613..19770)
                     /locus_tag="ACL8A1_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HGS1202014.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKTLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            complement(19855..20235)
                     /gene="rnpB"
                     /locus_tag="ACL8A1_001245"
     ncRNA           complement(19855..20235)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACL8A1_001245"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(20256..20585)
                     /gene="gpsB"
                     /locus_tag="ACL8A1_001246"
     CDS             complement(20256..20585)
                     /gene="gpsB"
                     /locus_tag="ACL8A1_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HGS1202015.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            complement(20655..21182)
                     /locus_tag="ACL8A1_001247"
     CDS             complement(20655..21182)
                     /locus_tag="ACL8A1_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HGS1202016.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            21248..21844
                     /gene="recU"
                     /locus_tag="ACL8A1_001248"
     CDS             21248..21844
                     /gene="recU"
                     /locus_tag="ACL8A1_001248"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HGS1202017.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            21841..24000
                     /gene="pbp1a"
                     /locus_tag="ACL8A1_001249"
     CDS             21841..24000
                     /gene="pbp1a"
                     /locus_tag="ACL8A1_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP1A"
                     /protein_id="HGS1202018.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLTYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGRNPEDWNIPEGLYRNGEFVFKNGARSTWSSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(24167..29470)
                     /locus_tag="ACL8A1_001250"
     CDS             complement(24167..29470)
                     /locus_tag="ACL8A1_001250"
                     /EC_number="3.2.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033926 -
                     endo-alpha-N-acetylgalactosaminidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpGH101 family
                     endo-alpha-N-acetylgalactosaminidase"
                     /protein_id="HGS1202019.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKEDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKSPTTSTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYDDERTVVSVKTDNQERVKTEDTPTQKETGPVVDDRKVTYDTI
                     QSKVLKAVIDQAFPRVKEYTLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTEKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLAFNYVKAYAHNTRRDNATVNDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HYPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVADD
                     FASLTAPAQAQEGLANAFDGNLSSLWHTSWGGGDVGKPATMVLKEATEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TNNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLRSLASDQGKTPDYKQEIA
                     RPETPEQILPATGESQSDTALFLASVSLALSALFVVKTKKD"
BASE COUNT         8479 a         6789 c         5256 g         9038 t
ORIGIN      
        1 tagactatat tgattactac aacaataaga aaattaagat aaaactaaaa ggacttagtc
       61 ctgtgcaata cagaactaaa tccttcggat aaattaattg tctaactttt tgggggcagt
      121 acataagaac cttggtaata tgcgtttttt gattgtgaag acttttatcc acctattata
      181 caaagtcaag tatccatcac agtttacaat tagccttcat tacttaaccc attcaccatt
      241 atagtttaag tagtaaccac ctactgtggt attaacagct aaagcacctg aatcataggc
      301 atagtaccag ttaccattga cttggaacca gcctgttttc atatctccat tacttgcatt
      361 tagatagtac caagtcgaac cttcttgata ccagccagtt gccattgctc ctgatgaacg
      421 gagatagtac cacttgttcc caaggtattg ccagccagtt ctcatatcac cattttcagt
      481 atctaaatag taccaagttg agccttcctt ataccagcca gttgccatag ctcctgacga
      541 atggaggtag taccacttat tacctagata ttgccaacct gtttgcataa tgccagttac
      601 tggatctaga tagtaccatg gagctgcttt tagcttctct tcatcatagg taagagggta
      661 atccttaacc caaccacgtg ctagctctcc aaccgtcaat ctgttgatgc gcgaatcaaa
      721 gccaccatcc ttctgtaaat aataccaatg accctcttca taaatccaac cagtttttaa
      781 agtatgataa ctacgctgat cttcaaaata atagactcgt ttctctgctt ggctatcata
      841 ttcttcccca tgatgtttgt tggtattcgt agcagttttt gcttctaaaa cttgcttgcc
      901 aacaaattct tgtaatacac catcttgacc aaaataaaac caatctggtc taagagcaat
      961 ctctattctt ggagaaggac caatcgtaac ccccttgtgt ggagcaggta tatattgcca
     1021 gccgacaacc atctctccgg ataaaggatc aaaataatag tacttcccat ctatcactct
     1081 ccagtaggtt tcttttagat cccccttttt gtagtaggtt ctaccatttt cttggacaaa
     1141 ctgccatcct tcagaatcat ctgcaaatac tgtactggtc cctagcaaac cgaagaaaaa
     1201 tgtggctagt gcgacttgca tcattttttt caaaagcttc aatttatata tcctccaaca
     1261 tcaaatccac tccccagatg aagcgaagtt ataaacttta ccatcgatag tttggctacc
     1321 tgtaaccatt gctccatttg aattgagata ataccagttt ccatttactt tttgccaatc
     1381 tgtaagcatc ttaccggaac tgtctaaata ataccaagta tcatttatct cctgccaacc
     1441 cgtttgttta tcatgttgtc tataatagta ccaactacca tttattttct tccatccttc
     1501 aagagagtaa cctttaagaa ccgaataaat aaatgacaaa tttcgttcat ttaatttaac
     1561 tgcactgtta atttgattag ctccatctcc taaagtatgc actcctacta cgcggtgact
     1621 agcatcataa actgcagatc cactagacct ctctaaagta tcaacttggt aatccaagaa
     1681 catgccatca tcacttaaaa cttgtttttt atctgtatac atttgataaa ttttaaaatt
     1741 atatgatgga tagcctgtga tagtcacagt tattcctgtc aaattttttt gactagtagg
     1801 aagacccaaa gtccctaatt ttgcaccaat gggcttttct agaattaata aagccaagtc
     1861 atattccctt gcatccttag aatttaaatt tctaaattcc ttcaaataac gaacttcctt
     1921 tactttgatc tttccaaatg gttcttgact tggactaaca gccagaagaa cataaatatc
     1981 atccgcttct ttcccatagt catgtctgta gtagttgtga gccgctgtaa ttaacacatt
     2041 atctttaata aaattcgcac ttcccctccc ttctacattc attccatcgg ctactccata
     2101 atacttagat aaaaccatag ctgaagtcga atacggatac tgtgaagtat tatcaatttt
     2161 aatcaaatca tcattaccga taatacttct gattgctttt ggtagtatga accatacgtt
     2221 ggtgaaatct cagataatga agaatcatta gaccctggac ctttttctag tgtctcactt
     2281 acctcatatt cttcacctgt actagaaata acactcaaag cagatactgt cgataactgg
     2341 ctagccaata aagtactcgc aataattgaa atacccaatt ttttataaac agttttcttc
     2401 attattgtat cctcctaatg taattatagc gtactattct aaatttctta atctactata
     2461 gaatcaagaa atctaccacc ttctttaaat accctccatt atcacataaa caggtaaact
     2521 tttcaattaa tgactgcgct tttcaatcac gctagaggta cttgcttgct tctttgatac
     2581 taagttcagc cattctttcc ttgtttttct caataaagca tgttacccaa gtgggattcg
     2641 ttttggagta gtctcgcaga gtccagccaa tggctttatt gataaaaaat tctgtttggt
     2701 tcaagttatg aagtagaatc ttttccatta attgagtatt ggtcttctct tttcttaaca
     2761 actggtggtc aatagcgaca cgtctcagcc agatattatc tgataggctc cattttatac
     2821 tcaatgaaaa tcaaagagca aactaggaag ctagccgcag attgctcaaa acactgtttt
     2881 gaggttgcag atagagctga cgtggtttga agagattttc gaagagtatt aagattattt
     2941 cttctagttc agagtgttca tacaccaaac tccctactac tcgatctagg atatctaccg
     3001 tgtcccacca ggattttgtc acgactaact gctctagctt aggcaaatcg gtttccttta
     3061 gataagactg cattgctttc aaatagttag cagccacata ttggtattct ctaggatcct
     3121 tttcccagca agtgtctgca aaatcccaat cgataatctt tgtttttttc gcttctggaa
     3181 aatattttta tagagtttat ttctttcagg caccgcaata cctagaaaag aaaattgatg
     3241 gcgcatatag gcttccatgg accttgcttt tttagagtct tttgctgctt ctagctcctc
     3301 aagtaaatct gctaaactca tctaaaactc atcttgcccc accaaatggt gctgaaaggc
     3361 atagacagcc gcctgggtac gatcgctgac ttcaagtttg gcaagaatgt tggacacgtg
     3421 ggtcttgacc gtcttgagag agataaagag gtcatctgcg atgcgctgat tttcgtagcc
     3481 cttggcgatg agttggagaa catctcgctc acgcgcagtc aattcttcat gaagttccat
     3541 atgattgcgg tggtattcaa ccttcttgct aacctcttgc tcaatggcca gctcgccagc
     3601 agctacctta ctgacggcat gaagcaattc atctgcacta gaagtcttga gcatatagcc
     3661 tttggcacca gcatctaaga ctggcatgat tttttcattg tccaaataag aggtcacaat
     3721 caaaatcttg gcttcaggcc attctttaag gattgctaag gtcgcgtcaa tcccattcat
     3781 ctcaggcatg acaatatcca tgacaatgac atctggacgc agttccaagg ccaagtcaat
     3841 cccttgagac ccgttggacg cctcacccac aacttctaca tcgtcttgga ggtcaaagta
     3901 gcttttcaag cccaatcgga ccatttcatg gtcatctact agtaaaattt tcatctttac
     3961 tcctttatca ttccttatct aacaggggaa tacggatatc aactgccagc ccttgcttgg
     4021 gagctgttaa taactgaacc gtccctgcca tatcttcaac ccgctccttg atatttcgca
     4081 gtccataact caagtcgtct aagctcccta accggaaacc aatcccattg tccaccacct
     4141 tcagttgcaa ttcaacatct gtctgataga ggtagacatc taggcaagat gcctgggcat
     4201 ggcggagcgt attgctaatc aactcttgca gtatacggaa gatatgctcc tcgattttct
     4261 taggcaattt cgtcatattc tgcttgagac taaccctaag atcactcttg tcctcaagct
     4321 cttttaagag aatctgaatc ccttctatca agctcttctg ctccagttca actggtcgca
     4381 aatgcaagag caaaacccgc aaatccttct gggcagtttc taaaatagct gtgacactct
     4441 gcaactggat ctgcatcttt tctctatcca atttcaaagc ctgctgactg atacccgata
     4501 aaatcatgtg ggccgcaaac aactcctgac tgactgtatc gtgcaaatcc cgagcaattc
     4561 gcttccgttc tttctcgatg atttcctctt cctgagcaag gctatgattt tcagcttttt
     4621 gaagagcttc tgtcaaaagg ttaagtttac ctgataagga cttgaaactg gcatccaaat
     4681 ctggatctgc aacctgaacc acttcttgcc ctgccaataa acgcttgaga ttagcctgca
     4741 tttttcttag agaaagctct tcgatccctc gccaaaacag ggctaaaaga caggttatgg
     4801 acatgctgaa aaccaacaat aaaaagacaa acttttctgt tttttcgaca tcgtgcaaaa
     4861 agatagacca gtcaaaatca agtatttcca gcaagctgtg ggagaaaaaa aagacaaata
     4921 ggaaggaggt gagagcaata atgacatagg cttgtttttt catcctctaa ccacctccac
     4981 atcaccaatc atagtggtca agaaaatctt gacactcttg ttactcttga gatagtcttt
     5041 tgtttcttga tgatagtgtt cattgcggag ggctcgcttg ggctggttga aaaaagtcaa
     5101 atccccatag agacagttaa cgctgagact gacttccaca tctacaggta cgatgatttt
     5161 ggtcgttcct accatctttc tgaggataat gacattgtca tgattggtta agatgaccct
     5221 ctccagatga atagtgtcct tgcccatgaa gcgaaagaga ttgatatcat cgaattggca
     5281 agtctggtag cttgaaaaat gatgaagatt tccaaaccaa cgatttttct ccttcttaac
     5341 cgtcacgacc tcttcaaaaa ccaaattggt ctgctctttt tcctggttca tcatcgggta
     5401 aagaagaaag aggctataga taaccgcaac aaaaatagct agaatcacaa aaggattgag
     5461 cataacgatg aaaaagaaga gaatggttgc cgctactaaa agaagattat ttccctcttt
     5521 accagtgtag tagcgaatca aaagcaaaaa gaggaatagt atcagcagaa aacgcgaaaa
     5581 atgctctgat accatcaaaa tcagagctcc tgtcagaaga caggcttcga taaataaaaa
     5641 gattttaaat tttctcatag gttcatcctc tcccttctat tttatcacaa ttcaaaaaag
     5701 tcacctcagt ctgaggatgg aaaaaaaggc gctggttacg cctttttcat ctgatccttt
     5761 gcttctttta attttccata aagaagatag tctacttttt gtagatctgc tatggtggca
     5821 cagttaaggg aacacataat caagcgtaga tctgctttcc agccttggac aatgccaatc
     5881 acttcttcaa ctgtgtaggt ttcaaccaat tccagaacgg ttcgtgacaa tcccacagcc
     5941 ttagcaccaa aaaccaagca cttaatcata tccagcggat tccgaacccc tccactaacc
     6001 aagagttcga ccttatcttt ccattcttgg gcattgagaa gggcctgcat ggtagactga
     6061 ccccattgat tgaggtaatc acgctggcca ctacgacggt tttcgatata ggcaaagctg
     6121 gtgccaccac gacccgatag gtccactgta cgaacaccga attcataggc tctttcgatt
     6181 gtcttggcat ccattccaaa gcccacttcc ttgaggacaa taggaacggg aatttgcttg
     6241 ctataatctg ctaaatgcga ttgccagctt ctaaacttcc tttctccctc gggcatgagt
     6301 aattcctgca tgacattgac atgcacttgc aatagaacag gattcatctc ttctacagtc
     6361 tgaagtccta actcgacagg cttgtccaat ccaatattgg ttccaaggag gagattggga
     6421 tgactagact tgacagaaaa agaatcatcc gttggatttt tgagggctgc gctataagaa
     6481 cccgttacaa ataaaatacc acaggattcc gccacctgag ccagcttttg attgatttct
     6541 cttcccttat tacttccacc agtcatggca ttgatataaa aaggaaagtc ccactttcga
     6601 ccagcaaact ctgtcgaaag atcgatttca tccagattgt aaagaggcaa ggaagaatga
     6661 atcagctcca cctcatcaaa gctattatag gaacttttct gctcaagggc atagaggata
     6721 tgctcgtcct tacgatttgt cgtcatgtcc tatcctttct tgatataaga gctcaatccc
     6781 cagatcggcc caacgatttt ttaaggtttt ggttgattgc gcatcaaaac tcagggcgat
     6841 gccacagtca ccaccaccag caccactact cttggcaacg gcctgcaaat cttgactggc
     6901 ttctttcaac tgtctaagca aaggcgtgta aatatctgta ctcaagcctt ctaaaagctt
     6961 gctggctact gctacttgct cgataatctt ttctgatttc ccctgttcca aggcttcgac
     7021 caaagaaacc accgtttctt ttgaggaagt taaaaaattt tgattgatat tttgcttgat
     7081 ttgctgaacc atgtgactcg atacagccac ttccttggtc catcccacta agaaatcaca
     7141 ttctaaagtt ggtttcactt gtgagataaa aaatccccaa tcacgctcca gaactgtcgc
     7201 caagttttct tcttctaacc aagcagccgc cttctggcga tcaaatgact ggtagagaac
     7261 caaatcctct gccacaatac aggcaaggtc gcccatggaa ccattgtctc ctcgcttgag
     7321 caagacagcg ctagtcagct tgaacaagag ctcctgatca acagaaacat catacagagc
     7381 cagtaaagcc ttgacaacca agacaacgac gctgccacta gaacctagac caaacttttt
     7441 cccttctcgt tccattttgc cacagatttc tagagaaaaa ggtcttaaat tctgaccgcg
     7501 aacagcgagg aagtctccca tcaaagcaat cgtttcttga atcaagctgt agtcaggatt
     7561 gggccttaag tccactgcga aatcaaacat atctgaatag atacggtagc tgtcagaaaa
     7621 agcaatctca gccctcatat agatgggaat atcctttatc aaagctaacc gccctggctc
     7681 taaaatagca tattcacctg ccaaatagag ttttccgcaa gttttaacag caatcatctt
     7741 gactcaaatc ctttgttttt gacacaatca agcgataacg ctgaccgaaa atttctgaca
     7801 aatgctccaa gtctttctcc tgacagaaga ccttaacatt gggaccagca tccatggtaa
     7861 agtagcaggc ctctcctttc tcacgaagct gacgaacaaa ggccatagcc tcataagagg
     7921 catccgtcag ataagaaaag gctggactag cagtctttgt cgtagcatgc atagccaggg
     7981 catttttctc cgttaattct ccaatcttgg caaaatcatt ttccttgaga taaatcagca
     8041 tatcctgata gtccttctca gactgacgaa cccagtcgtc gaaagtcgtc gaggtttcca
     8101 cacaaagttt catcccgtca cggctagaga ttggtttttt cttgtcctct agcaccaaca
     8161 taatcatagc tagtttcaag tctgtctcta cagggtaaat ttctccacta tccttatccc
     8221 aggctcctag tggtccataa aaactccgag aagaagagcc tgaggcaaat ttggcttcct
     8281 gtgccaactg acttctatcc aatccaagtt tgaaataagc attacaagcc ttgaccaggg
     8341 cggacaaacc actagaactt gaggacaggc ccgctgccgt aggcatattg ttttgagtat
     8401 cgatacggac aaagccctca ccagctggac gataacggtc aataatctta ctcatcttgg
     8461 catgctcgac ctcattttgt agctgaccat tgatgtaaaa ttcgtcagct gttacattgg
     8521 ctggtaaagg cgacaaggtc gtctctgtat acatattttc caaagttaga gaaatactgc
     8581 tagtagcagg caccatctct ttttcttttt tctttcccca atatttgata atagcaatat
     8641 ttgcgtagga acgtactgtt acaggctctc tatccatgtc tgaacagctc ctttctcttc
     8701 taatctttct gctagttctt gtgcgtgtgt caaattggtt accaaggcta tgatacaacc
     8761 tcctagccca ccaccgctca tcttggcacc cagagcacca tggctaagag tcgtttcaac
     8821 caaaaagtct gcctcagggc tactgactcc aatttctttt aaatgtaaat gcgcttgact
     8881 gaggatttgt cccagtcctt cagcatcttt ttgtgaaatc gcaacttctg cttgctgggt
     8941 taattctccc aaggcatgca aaaacggtag ggcatccttg cccttatttt gaaccacttg
     9001 gatggcttca cgagtatgac cataaacacc cgtatcggca atcaccaaat aggcggataa
     9061 atccatctca agttctgtaa atcctacgtt cttgataaag cgaataggtt ggtcactgag
     9121 acaggtctta gcatccaaac cactaggatt catatgggca atcatctcag ctcgattgac
     9181 caagatttct agtacatcat gaggcagatc agcctgatag tagtcaaata ccgcacgaat
     9241 ggccgctatg ctgatagccg ctgacgaacc catcccccgt ttctcaggga tagccgagtc
     9301 aatctcacaa cgaacgcagg cttctgtgat atccaaatac tccagtgagg cataaaccgc
     9361 catggacaag gtatcctcct cataaaggcg ccaaggactc tctgcagaaa ctaccttaca
     9421 ggtcacctcc acctccaaaa gaggcaggga aatggcagga taaccgtaaa cgaccgcatg
     9481 ttcccctatt aaaattatct tactatgtgc ctgaccgaca ccaacttttt ttgtcatttt
     9541 ttccttttac tagacgaaaa aacgtcttat ttttcataca agtattaatt ctttcctatc
     9601 tattttatta tattttcaca aaaaaagcga ttgtttccat tcacaatcgc ttctttcatt
     9661 attgaaccca ttcgccatta taattgacaa aatagccatc tacggtcgta ttcactgcca
     9721 aggcacctga gctataagcg tagtaccatc tgccattgac ctggaaccaa cctgtcgtca
     9781 tagaacgacg aaagaaactc cataccatta agtaaagagg aaagtcgtga gggagcatgc
     9841 gccattgaca acctgtttta gtgacgtaca aagtctcatt aacaagtact cgtttcggcc
     9901 atttataggt gcggtgtttg gagaaatagg gttcaatctt agcccattct tgatcgttta
     9961 aatcagtatc atatactttg cgtgtcataa ctctagttta acattttttg tgaatacagg
    10021 ttctaaataa tcgaccacga aaatttctta agtggaaaac gccttatgaa gtatgctacg
    10081 ggaaagttat gcacttaatt tgacaattca agatgtaaaa atatatacta tagtagatta
    10141 aaactagaat agtacacctc tacttctaaa atattgttag aaatcgattt gactgtcctg
    10201 atcgatttat cctgttatta tctcattttg ctataatatt tgataagtta tcctaaaagt
    10261 attattatgt tgttgtgtta tagattgatt gaatctaact aaaggatcct attcaattac
    10321 tagaactatc acatactcaa ggtcagctca cagatgagca actattttgg ttacaatgtc
    10381 tactaaattt aagtcaaaca aataatttag tcaaaattaa aaaaatagag gaacataaat
    10441 atgattacaa aacagaatgt aatagtgttc tacaattttt actagataaa actgtaaatt
    10501 ctgaaggaag gatcacttct tcaacagaat ttggaaattt cgtaagtaat ttatcattcc
    10561 aacacggaat agctggacta ctgtttcctc taaataaatt gtacccccca gaactggatt
    10621 ctaaaatact ctctatcatc aagaaggcag tgacaattag aacgacacat acatatgaat
    10681 atcaatactc actgctattt ggtgatgcag gctatctatg gttactccta catttatttt
    10741 ctatcagtaa aaatcaatac tatctacaat tagcaaacgt caccgctaaa aaattaatag
    10801 agaattatga tactctagag gaaatagact ttgcattggg aaaatctggt gtcctattat
    10861 cattaataaa atactatcaa tttaccaatg acaatactct taaaattttc atccacaata
    10921 gtatagggga aatttatcat tatttcctac aaagagatac agccaaagaa agcattttag
    10981 actatagctt tgctcatgga tattgtggaa ttgcatatgc tttatttgct tattctaaag
    11041 tcttagaacc ttctatgttc tataatgatc tccatacatt ccatactgaa ttaaaaaaat
    11101 tattagaaaa agttacttct aatactgaaa atttaggaaa tttacaactt tcttggtgca
    11161 aaggaatttc cggaataatc ttatatcttt gtatgtacga ttgtgacgga aacaaagata
    11221 ttattagtaa atatcaagaa tttgttttta accatcatct aaaaatgatg acaggatatt
    11281 gccacggaat aactagctta ctacaaacca ctgtctacaa tcaaaacaaa ttactgatga
    11341 aaaaaatcca acaggtaatt ttagcatgtt ctgaacgaga tgatcacggt ttactgatgt
    11401 ttcaaggaga tagtggtaaa gcagatttgt ttgacttcgg aataggaagc atggggtata
    11461 ttggtgtcta ttaaataata aattcccatt cgatgtgcag acataaggag aaaagtatga
    11521 aattattttg gacaaacaac atatatagac agttgctgct aaacagctgt ttttcatcat
    11581 tcggcgacag tattttctac ctcgccatta tcaattatgt ggctcagtac aatttcgctc
    11641 cgctagcgat tttactgatt tccatttcag agatggttcc cctactatcg caactctttc
    11701 tcgggattct aggagatttt caagaaaata gagtcaaaca cgcactctgg attgccaaaa
    11761 tcaaaatcct gctctacgct attttgacag tatttctcgt cttgtcgccc ttttcattag
    11821 tttcagtcat tatgattgtc atcatcaacc tcatctctga caccttgagc tacctgtctg
    11881 cctacatgat gaacgccctc tacatcagtg taattaagga cgacctgcat gatgccatgg
    11941 ggttcaggca gtctctgatg agggttgtcc gtattgtcgc caatctggct ggcgcattcc
    12001 ttatcaatgt tataagtatt caaactattt cccttatcaa cactctgact tttgtcattg
    12061 cctttttggg cctgtatgtt attcgacaca ccttgtatga ggttgaaaaa agaattgaaa
    12121 tgtcacatac agcactgagt tttaagaaat attttcaaca tcttaaacag tcgctggctg
    12181 tgctcctgag gttaaaagat accgtcatac tactgtttct gacgaccagt atgattgcca
    12241 tcttggatgt gtcccctcgg ctgattgccc tccgcttcat ccaacagaca ctagcacaac
    12301 tgagcattgg gcaactcctc gccctgctct ccatcatcat gtcttgtgga gctatccttg
    12361 gcaatatgac cagcagtaat ctatttaaaa atatccgttt cacgcacctc ttggttttct
    12421 gtgagatttc cctattgact ctaataacta gtatcctttg tcaagcctat atcgtaattt
    12481 tcatgaccag tttcatcagt tctacgatta tcggcattct cagccctcgc ctacaagcag
    12541 ctgtctttgc ccatatcccc agtgacaaga tggggacggt gggctctgct ctgagcacag
    12601 tggacattct cgccccgtcc ctgctctccc tattagccct atccatagca tcgggcgttt
    12661 cggtgcagtt agcattgata tttttgtatc ttattttaat tgctcttatc ttttgtcaat
    12721 ggttagtcaa gttcaacact cataactaac gaaaaagcat gtgtagattt cacatgcttt
    12781 taatctcccc aatcgtcagg tcaagtacaa caaagtcact tctttgatta agcgagtgtt
    12841 ctaatataat tataagcgcc ctgtcattac cgaacccatt cgccattata gttgacagaa
    12901 tagccatcta cggtcgtatt cactgccaag gcacctgagc tataagcgta gtaccatctg
    12961 ccattgacct ggaaccaacc tgtcttcatg tctccattac ctgcatttag gtaataccaa
    13021 gttgagtcat ctttcaccca gccagtcacc atggcgcctg acgaacggag atagtaccat
    13081 ttgttcccaa ggttttgcca tcctgttttc atatcgccat ttggctggtc taaataatac
    13141 caagtggtac cttcctgata ccagccagtg gccattgctc ctgaggaacg gaggtagtac
    13201 cacttattac ctagatattg ccaacctgtt tgcataatac cagttgttgg atctaaatag
    13261 taccaagtcg aatcatcgtt tatccacccc gcacgtcttt caccaccaag gtagttttct
    13321 ccattaattt ccgtcttagc tagataatac cagttagact gatcataaag ccaacctgtc
    13381 tctaaagaat gattttgatt aaagtaatag ttcgtataat aacgtttctc ttctttatct
    13441 tctgaatctt cacgtttttc cccgtacttt cttccaacac tgtctttagt tttaatctct
    13501 aatgttttcc aaccaacaaa ctcttgtagc actccatttt tatcgaagta gtaccactct
    13561 gactttggaa aaccttctaa tctgatacca tttgggtaag gaccaattgt actaccttta
    13621 gatggaaacg ggatatattg ccagccgaca accatctctc cagatagaga atcaaaataa
    13681 tagtacttac catcaatcac tcgccagtag gtttctttga ggtccccctt tttgtaatag
    13741 gttctaccgt tttcttggac aaactgccat acttcagagt tatctgcaaa tactgtattc
    13801 gtagctagca aaccaaagaa aattactgtt agtccagctt gcatagtttt tttcaaaagc
    13861 ttcattttat atatcctcca acattaaatc cactcaccag atgaagcgaa gttataaact
    13921 ttaccatcga tagtttgact acctgtaacc attgctccag aggaattgag ataataccat
    13981 tttccattta cttttgtcca acctattttc atatcaccat tttcagcatc taaatagtac
    14041 caagttgaac catcttgata ccagccagtt accatagctc ctgatgaacg gaggtagtac
    14101 cacttgttac caaggtattg ccatcctgtt ttcatatcac catttttatt atctaagtaa
    14161 taccatttgc ctcgtacata tttccaacct gttgccatag aaccagagga gccaaaataa
    14221 taccattttc cgtttacttt ttgccaatct gtaagcatct tacctgaagc atctaggtag
    14281 taccatggag caggtttgct tctatcaatg tcaatagtaa catgaaaatc ttgagtccaa
    14341 cccttagcaa cttcaccaat tggtagtgga ttgtaagaat catcgccaaa atttcctagc
    14401 ttatttaaat aataccaatt gccgtcttca taaatccagc ctgtctttac agcataatta
    14461 ttatcaaaat aataagtttt cttttcagca gatagaggat attgttcacc gtatactttt
    14521 cctgtatttt cagacgtttt tgcctctagt acttgtttat ctgtttgttc tagcaaagca
    14581 ccatcttgtc caaaatagta ccacttctcc tgaaggccaa tttcgaagac tggacgatta
    14641 tcaaataaat catcacgata acctgttcca tgaatttcta agtattgcca accgacaacc
    14701 atctctccaa tatcaccaaa atagtaagtt ttaccgtcta ttttatgcca atagattgct
    14761 ttatgatcat cttttacata atattttcta ttatccttat caacaaatcg gccacccatg
    14821 gtatctgcaa atactgtact tgtcgctagc aaaccaaaga aaaagactgc taatgcgatt
    14881 ggcatcattt ttttcaaaag cttcatttta tatatcctcc taaaacgatt gtagtaaaaa
    14941 ctagattgta tgtcaatata taaaaactcc tcaaaaaagc agttacacaa ctgcaactgc
    15001 ttttctattc ttgcatggtg taaccaacac cacgcacggt tttaatgtag cttttttggc
    15061 ctttaacatc aagcttgctc cgtagatagc ggatatagac atctacgata tttgtctcgg
    15121 tcgcactttc atacttccaa acactttcca acaattgctc acgagtcaat actttcttac
    15181 ttcccataag tgtcgccaaa aggtcatact cacggcgggt cagagcaatc atctcttcac
    15241 cacgataaac cgtgtgatgt tcaacatcta tcctaagatt gcggtaggtc cttggaactt
    15301 tcatcagact gcagtgttga tcaatgaagt cccgacctcg gaagatcgcc gaaatacgcg
    15361 ctaccagatt ttcgataaag actggcttat agatgtatga aactgcaaaa cgctgaacaa
    15421 cttccagctc ttcttgcaag tcttcccaat gatctaaaat catgatgact gaggcaggtt
    15481 tagttcgact caatttttct gcaaaatcct gagccatcat atctcccaga ttaacgttca
    15541 ataaaatcaa atcatagtct gtctgaagag ccatggaaag ggctttttgc ccctcctcta
    15601 ccagatcaac ccgatactgc tctttctgga gttccaaact taaaaaatga gctagatttc
    15661 gttctttctc aaataataaa atccgtttcc ccatggctga cctacttatt tttcgtcata
    15721 ccaagagtag tggaaggttc cttctttgtc tttacgttgg taagtgtgag caccaaagta
    15781 gtcacgttgt gcttggatca agttagccgg aaggtcagct gaacggtagc tatcaaagta
    15841 agtaatagct gctgagaaag ttggcactgg cacaccagct tgaaccgcaa gagctacgat
    15901 atcacgcact gcttgttgat acttagcagt aacatccaag aagtactcgt ccaaaagaag
    15961 gttggcaaga tctgcatcgc ggttgtaagc atctgtaatc ttttgcaaga aacgagaacg
    16021 gatgatacag ccatcacgcc agatagatgc gatatctgca aatggcaagt tccagttgtt
    16081 ttctttagag gctacacgca attgagcaaa tccttgtgcg tatgaaatga tttttgagaa
    16141 gtaaagggct tgacggattt tttcaatcaa ttcagccttg tctccttcaa agttgaaggc
    16201 agctggtttt ggaagcacct tgctagcatg tacacgttct tctttgtaag ttgaaatgta
    16261 gcgtgcaaac actgactcag taatcagtga caatggtaca ccaaggtcaa gagatgattg
    16321 gctcgtccat ttaccagttc ccttgttacc tgcagcatca aggatgtagt ctacgattgg
    16381 tccatcttgg tcttcatcgt ctttacggct caagatatca gctgtgattt cgatcaagta
    16441 gctatccaat tcacccttgt tccactcagt aaagatttca gccatatcct ctgcagaaag
    16501 gcctagcaag tgttgcatca agtcatagct ttctgcgatc aattgcatat caccgtactc
    16561 gataccgttg tgaaccattt tcacatagtg accagctcca tcaggaccga tataagtcac
    16621 acatggtttg ccatcttctg gtgctttagc tgagatttct tcaagaacat ccgaaaccaa
    16681 ttcgtaggcc tctttttgtc caccaggcat gatagaagga ccttcaaggg cacctttttc
    16741 accaccagaa actccagtac cgataaagtt gatacctgag tttgccaatt cttcattacg
    16801 acggatagta tctttgtaga aagtgtttcc tccgtcaatc aagatatcac ccttgtcaag
    16861 gtgtggaaga agggcttgga tagtagcatc tgtaccaggt ccagcttgaa ccatcagcat
    16921 gatacgacga ggtttttcga ttgagtttac aaaactttca acgtcatagc ttggtacaaa
    16981 gttcttttca ggatggcaag caatcacatc ttccgttttt tctttactac ggttgtagat
    17041 agcaactgtg taaccacgag attcaatatt aagggcaagg ttacgaccca ttacggccat
    17101 acctacaaca ccaaagttag cttttgtcat ttgacactcc tcttgtttca tttgttttat
    17161 tttatcattt ttacttttga aaagaaagaa ttttgtaacg actgcttagt agtccaagtc
    17221 atccgcatga ccagcgccat ttccgacaaa gtagcctgtc ttacagttaa gggtaaagag
    17281 ataggttcca tctggcttat agaggttgta ataaccattg ccgttaacga tattgacacg
    17341 ttcaaggata tattggtctc cagtgatata gccacgtgaa cgtgaagtcg ctagaatttg
    17401 ttccaagaca ccatccgcaa aatcccaggc agagttatta ctatcatcaa tagcagactg
    17461 attataggca acacgactgg cacttctttg aacagcaacc cctgcactcg agagacccat
    17521 attgacttca ctgcgactac ttctagtctc atttgaacta cttggttttg gctcacttgt
    17581 cgtatttgag cttgcttgac tagagcttga gctgctagtt tgacttgaac ttgagctaga
    17641 agtacttgtt tgctggctct taccaagact gatagcctta tctagcactt tatcaagctc
    17701 cgtattccca gttttaatat ctgtaaattt agcatccgat ttagctttgg cattggtatc
    17761 caacacacca tccacaatag ctgatttttc aaattgtgca ttgacatctt gaatagcctt
    17821 gatttgcgtt gctagactat catatttaga tttggcaagc gtatgttcac gactaccttc
    17881 cagcttatca agcaaggttt tgagttgact cagtttatca aactggctat ttttcaaagc
    17941 cgttttattg ctatctgtgt aaaaggcgtc ataaagtgta ttaaaatcat ccacatctga
    18001 ttgattggct gtagttgatt gagaagtttc aatttccttg gtcgagcgat tcacttgacg
    18061 gtagacataa taagcactga cacaaataat tactgataca agcgccaaaa cggtcaaaat
    18121 aaagcctttt ttacttttct tttctgagtc ctcttgatcc agacgagaaa ggttttgctc
    18181 ctcttccact tcttcagtca tttctgttgg attttcatca tctagcaata gaggatccaa
    18241 gccttgctcc tcatcgtcca gaggcaaggg tagtaagatg tcttcagaag atggaacttc
    18301 ctccgaagaa gcttctgact tctctacagc ctcacgcatc tcttgaatta aatcatccag
    18361 actctgagtt tcaacgaact cctctttttt gtattgacga gtcgcaaact tatccgcctc
    18421 aatttcatct ctgtgttgct taacatactt gtccaaaatg gaatcctcag gcaagactcc
    18481 tgcttccact tcttcatttt tacgaatagc ttgaccaact gttagctctt ttgcttcatc
    18541 aaaatcaaat tgcggttctt gaccttcttt tttatgacga tttcgtcttt ttttactcat
    18601 gagtatccct ttctatttta cctcttgccg tttgacacgc tgaccaaaat attgatatag
    18661 atccactttc aaggttccgt tgtataactt acgcttctta tctgcttggc taccatactt
    18721 gctttcaaaa gcttcatcac tagtcaggat aaatttgctc caagttttca gcggtgcaaa
    18781 tacttgcccc atctcagcat agagcttggt cacccctgca tcatctgaca aacgttcacc
    18841 ataaggcgga ttggaaatga ttactccatt gattttatcg gaacgtaaat cctgcacgcg
    18901 catctgctta aaagtaatgt ctcctgcaac accagctgcc tgagcattgg ccttagcaat
    18961 ttccaccatg cgagcatcaa tatcacagcc catgatatcc agctcaagct cacggtctac
    19021 ttttttagcc gcttctgtgc gcacttcttg aatcaagcga tcgctgatcc agttccattc
    19081 ctcaaatgca aaagagcgac gaagacctgg cgccatcttt ctagcaatca taactgcctc
    19141 aatacagaaa gtccccgaac cacaggtcgg atcaatcaaa gtcttgtctg gataccaatt
    19201 agaaagttgt aaaatggctg ctgccatatt ttccttgata ggagcgccac ctttttcggt
    19261 acgataacca cgtttaaaga ggctagaccc ggtcgtatca atcatgacag ttgccacatc
    19321 tttgagaata gagacctcaa tcttaaactc tgggccattc tccatcagag gaaccccttc
    19381 tgggcgagca tagtgtttct gcaatttctt gacaacagct ttcttagaaa tagcctgaac
    19441 actgggctca ttgtgaagtt tggacttaac acatttagct tttgaaatcg ggaaccgagc
    19501 tccaagtggt aaataatttt cccaatccaa agcgaaaact ccctgaaata gctcttcaaa
    19561 agtcttagct gggaacgttc ctacgataat tttgatacga tctgctgccc gaagccaaag
    19621 gttggtttcg ataatagctc tcacgtctcc ttgaaaacga acacgtccat tttcaacctg
    19681 acaatcgtag cccaactctc gcacttcacg accaacgaca gcctcaagcc ctgctgccac
    19741 agttgcaatt aaattaaatt cttttttcat gttacagtct tgcaagacct ttctatatgt
    19801 ccactttaaa aaatgaggtt gagaaaattt ctcagcccca acctatatgc aattttctat
    19861 aagccatgtt ttgttccaga agtgactagg accacttcct tcgataatca tctgtctacc
    19921 actaacagct ctagctgata gcagtcagaa tactacgttc gtttccgcgt actccatgcc
    19981 ccgaccaaaa tttgggttgc tagcttgagg ggtttaccgc gttccactct ctctgtttcc
    20041 agaaagactc cgtcactgtg gcactttcaa gcctactctg gcctatccaa ggacttagcc
    20101 atttcaactg ccgtaacgat tcctcgtccc taggcttatg gtttcgccta gcacaaacac
    20161 tacaggcatc acagcctgtg ctagcatgga ctttcctcat gagaagcaat tctcctcacg
    20221 cgattatcca aaaattgcac atctcaaata actacttaaa aatctgagtt atctaaaatt
    20281 tgtttaccaa aaacttcttt ttccaatcta ttcaggcgtt tcaaaatatc aaaattcgtc
    20341 atagaacttg taattgccgc ttcaaggggt tctgcttgaa ctggtgaagg tttcggttta
    20401 cgagttaatt cttccttcaa atccgcaatt tcctgacgaa gtgacttgac caaggcagca
    20461 taggtttcat agtccttgat gacatcgtcc aaaaactcgt caacttctac tttattatag
    20521 ccacggactt cacgtccaaa ctcttgttca aaaatatctt tcgctgaaaa aataatactt
    20581 gccatgtctc tctccattct cgcttgctag tattattata taaaaaaagg caaacaaaaa
    20641 tcaaggtcaa agcttcaatt ttcggaaaaa ttttcagcaa gttcatttaa ttgatcaaat
    20701 gttaatctct ttataaaata gtcctcttga tttttcatct tttggtaaaa ataagctagt
    20761 tttgtttcat tttcttcatc ataaaaaaga taagaactag tcgtgttttc cagcaaaaac
    20821 tgctggtaat ctcttaactg ccccttgtgt tcatagcgag gataagcata tttgacaaag
    20881 tctacctgct taaagcgact cagtttcatc tgattgcctt cattccaatt ttccccatgg
    20941 gtttcaaaag caaaaatggt ggccaactgg aaaccgtatt cggttttcat ctcctgagca
    21001 acctctaaaa cccaatattc aaaacccaaa cttcctgtaa acacaagcca agacacccca
    21061 tctgctgcca tagcctctaa atctttacgt atcgcttttt taatcaattt aagacgagga
    21121 tccttatcag aaaataaacc caaatcaaaa gcagaatagc ctaaaaccaa agctgtagcc
    21181 atttttaacc ctttctgtgc aaattatggt ataataaagt gatgttattg taccaataag
    21241 gagaattatg gtcaactatc cacataaagt ttcatcacaa aaaagacaaa catctctttc
    21301 tcaacccaaa aatttcgcaa atcgaggaat gtcttttgaa aagatgatca acgctaccaa
    21361 cgactactat ttgtctcagg gcttggctgt tatacataag aaaccaactc ctattcaaat
    21421 cgtacaagtg gactatccac aacgaagtcg tgccaagatt gttgaagcct attttcgaca
    21481 agcttcaacg acggactatt ctggcgttta taatggatat tacatcgact ttgaagtcaa
    21541 ggaaacaaaa caaaaacgtg cgattccgat gaaaaatttt catccacatc agattcagca
    21601 tatggaacaa gtccttgccc aacaaggaat ctgctttgtc cttcttcact tttcttctca
    21661 gcaagaaacc tacttattgc cggcattcga tttgattcgc ttctatcatc aagataaggg
    21721 acaaaaatca atgccacttg aatatattcg agaatatgga tatgaaatca aggctggtgc
    21781 cttccctcaa attccttatc tcaatgttat caaagaacat ttattaggtg gtaaaacaag
    21841 atgaacaaac caacgattct gcgcctaatc aagtatctga gcattagctt cttaagcttg
    21901 gttatcgcag ccattgtctt aggcggagga gtttttttct actacgttag caaggctcct
    21961 agcctatccg agagtaaact agttgcaaca acttctagta aaatctacga caataaaaat
    22021 caactcattg ctgacttggg ttctgaacgc cgcgtcaatg cccaagctaa tgatattccc
    22081 acagatttgg ttaaggcaat cgtttctatc gaagaccatc gcttcttcga ccacaggggg
    22141 attgatacca tccgtatcct gggagctttc ttgcgcaatc tgcaaagcaa ttccctccaa
    22201 ggtggatcaa ctctcaccca acagttgatt aagttgactt acttttcaac ttcgacttcc
    22261 gaccagacta tttctcgtaa ggctcaggaa gcttggttag cgattcagtt agaacaaaaa
    22321 gcaaccaagc aagaaatctt gacctactat ataaataagg tctacatgtc taatgggaac
    22381 tatggaatgc agacagcagc tcaaaactac tatggtaaag acctcaataa tttaagttta
    22441 cctcagttag ccttgctggc tggaatgcct caggcaccaa accaatatga cccctattca
    22501 catccagaag cagcccaaga ccgccgaaac ttggtcttat ctgaaatgaa aaatcaaggc
    22561 tacatctctg ctgaacagta tgagaaagca gtcaatacac caattactga tggactacaa
    22621 agtctcaaat cagcaagtaa ttaccctgct tacatggata attacctcaa ggaagtcatc
    22681 aatcaagttg aagaagaaac aggctataac ctgctcacaa ctgggatgga tgtctacaca
    22741 aatgtagacc aagaagctca aaaacatctg tgggatattt acaatacaga cgaatacgtt
    22801 gcctatccag acgatgaatt gcaagtcgct tctaccattg ttgatgtttc taacggtaaa
    22861 gtcattgccc agctaggagc acgccatcag tcaagcaatg tttccttcgg aattaaccaa
    22921 gcagtagaaa caaaccgcga ctggggatca actatgaaac cgatcacaga ctatgctcct
    22981 gccttggagt acggtgtcta cgattcaact gctactatcg ttcacgatga gccctataac
    23041 taccctggga caaatactcc tgtttataac tgggataggg gctactttgg caacatcacc
    23101 ttgcaatacg ccctgcaaca atcgcgaaac gtcccagccg tggaaactct aaacaaggtc
    23161 ggactcaacc gcgccaagac tttcctaaat ggtctcggaa tcgactaccc aagtattcac
    23221 tactcaaatg ccatttcaag taacacaacc gaatcagaca aaaaatatgg agcaagtagt
    23281 gaaaagatgg ctgctgctta cgctgccttt gcaaatggtg gaacttacta taaaccaatg
    23341 tatatccata aagtcgtctt tagtgatggg agtgaaaaag agttctctaa tgtcggaact
    23401 cgtgccatga aggaaacgac agcctatatg atgaccgaca tgatgaaaac agtcttgact
    23461 tatggaactg gacgaaatgc ctatcttgct tggctccctc aggctggtaa aacaggaacc
    23521 tctaactata cagacgagga aattgaaaac cacatcaaga cctctcaatt tgtagcacct
    23581 gatgaactat ttgctggcta tacgcgtaaa tattcaatgg ctgtatggac aggctattct
    23641 aaccgtctga caccacttgt aggcaatggc cttacggtcg ctgccaaagt ttaccgctct
    23701 atgatgacct acctgtctga aggaagaaat ccagaggatt ggaatatacc agaggggctc
    23761 tacagaaatg gagaattcgt atttaaaaat ggtgctcgtt ctacgtggag ctcacctgct
    23821 ccacaacaac ccccatcaac tgaaagttca agctcatcat cagatagttc aacttcacag
    23881 tctagctcaa ccactccaag cacaaataat agtacgacta ccaatcctaa caataatacg
    23941 caacaatcaa atacaacccc tgatcaacaa aatcagaatc ctcaaccagc acaaccataa
    24001 catttatcat ccagattttt ctgggtgatt ttttcatagc actattgtat ttatgtgctt
    24061 aacaaaagcc tgagaagata tcttctcagg ctttttctgt actataaacc tcattggaaa
    24121 agatagagac tgcttccgta atcttgttaa gaggttttac taaatactag tctttcttcg
    24181 tttttactac aaagagagca gatagggcta ggctaacact tgctaggaag agggctgtgt
    24241 cagattgact ctcacctgtt gctggcaaga tttgttcagg tgtttctggt ctagctattt
    24301 cttgcttata atctggcgtc ttaccttgat cggaagctaa agatctaagt ttaaactcag
    24361 gtttctctac agttggagca tctggtttcg tagcagaatc ttttaattgg ttgagataat
    24421 ctgcaaagta ttccaccatt ctttccgtca agagatggtt attcgtcgca tatttcatgc
    24481 ttgcctgaac gctagctact tcctcttgat tgtctttgtc tgttaatttc tgagccttag
    24541 ccaaagctgc ttcatagcct gacaagtcaa gaggtgtttc tgctacctgt ggacgagtaa
    24601 agatgagttc cgctgcagat tggtatttat cgccaccatc tccgtaagtc ttagtacctg
    24661 taaggacaat tttcttagcc ttaattgtct taccaaaatc aatgtctttt ggcttattgt
    24721 tatctggcca atcagttgca gtaaaggtat gctccttgcc agactcatct gtcacaacaa
    24781 gtttcacatc acgcaagtta ccatttgaac ctgatccacg tggaacataa cgaagtccag
    24841 tgatttcagt tgcttctttc aagaccatgg ttgcaggctt gcctacatct cctccgcccc
    24901 atgatgtatg ccataaacta gataagtttc catcaaaggc atttgcaaga ccttcttgag
    24961 cctgagcagg agctgtaaga cttgcaaagt catctgctac caaagccgtt ttcttctgaa
    25021 ccaaagcatt cttcaaggct tcaatcttgg caatctctgc acgcgcttct tccacactga
    25081 tatcatcatc ggcctgactg aggttaaaga ccgcctcttt caaagcatcc atagactctt
    25141 tggtgtagtt agtcatggca accgttggca agtagttctt cagagcattt tctgtcaaca
    25201 tcttacctgt tagggtaatt tcttcgattt gaagattatc catcatgaag tcgttataac
    25261 cacggaagtt ggcatttcca ccagaatcac cacgagtatt acttgcattt ccagttgagt
    25321 agatacctac ccaagtatcc cctgtttctg cacctgtcac gaggaaggtt gccttcttgg
    25381 ctttcttaga atctgtccaa gtatttggca attcatgcat ttccaagttg cttgcttgag
    25441 tgccacgacg acctgactgg aattctccct taccgactac aaaagcatag gtattgtctg
    25501 aacctgcttc gtattcaaag gttacacggt aggtcttacc tgcttcaaaa cggaagtttt
    25561 gcggaatagt ttggtaaacc aagttacgac ggctcactag tccatttgtc ttgagtgacc
    25621 aatttccttc gataacatca tcgactttct taccattcca accacgttgt gtatatggat
    25681 agtgtttttc agacaagtga gtgcggttat cttcaacacc ttcaacacca cccactacaa
    25741 atgggaagat accctgagca acattttcaa agtcttgctt gaaggtgcct ttacctgtat
    25801 catgcttgtc tccatacatg cttgaattgt tttcaaaggt acgaatttca tcaaagtaag
    25861 ttgcttgatc accagcttca cgactcaatg tcagagtaac atttgagacg tccgatcctg
    25921 ttgtaaagaa ggcgtacatg ttttggaagt aacttgtatc gttaactgta gcattgtcac
    25981 gacgtgtatt gtgggcgtaa gccttaacat agttgaacgc gagagactta ttggtataag
    26041 tagtcacttc tttttcacca gtatttacag tgatgctcgc cttggcatta ctacggttat
    26101 cgacaccgac ataaacggca tacttggtat ttggtttcaa gccagttaat ttctgagtga
    26161 gactaacttt ttctttgttt ccttgaatac gaagcatatc gtttgcccct tgagacttga
    26221 caatttctgc cttagaagca tcgcctgaaa tggtccaatg tttcaaggta ccactgttaa
    26281 atccttggtc atagatgtgc atgccttcac tccatgacat ttcaggattg gtttgttttg
    26341 aacgatagag aacgtatggt tgatttgcta gaagatctag ggtaatttta ccatctttta
    26401 cagttagttc ttgctcttct gtcttacctt ggtcagttag cttgtaaagg taaaccttgc
    26461 tctttgccca atcgcttgga agggtccaag ttgttgcacc ggcctgcgta ttgaagtagt
    26521 acatcttttc cttctcagta gaaagtttct taccatttgc atcccagttc caaggagtca
    26581 agtaagctga accatcttgg atgacacgtc cgttgagagt tactgtacgt tcgcgatatt
    26641 gtgggctatt gacatcattt gacttacgag ttacaactac tttattattg tcagcatcta
    26701 ccaattccac tcgcatttct ggagtccatt tataggtgct accgttatcg gtcatagtca
    26761 ccggtgtacc attttcccat ttacttacag tgaagtgttg gaaatacttg gtcatgacat
    26821 catgggcaaa gaggttagtc acgtagccat tgtagtcgct ccgtccttgc cagccttcaa
    26881 agtctttcat gctgtagcca cctagcagtg gatagttggc tgcaccacca taacttctgt
    26941 agtccccaac ccaagcatct ttttggtggt tacggataaa gcgggtgatg gcactgttga
    27001 tacctttatt ggtgtagcca ccgtaggtca agtcagctgc ccagtgatgg aaggtagagt
    27061 cgtactcacc accatggccc cactcgatcg caaagcgcca gccttgtttg ttaatttctt
    27121 tggcaagaac gtgggtagcc caggcaccgt tatcacctga ttgaccatta ccccaaacgt
    27181 ccacatagat aaagtcgaga ccgtcaccaa gttttttctt caaatcttcc caacgtgcca
    27241 aacgaccatg agctaggtca taggcagcat caatgttgat accttgatct agccagttcc
    27301 aaccatagct atagcttcca tctggattct tacggagaat tttttcattg aagtatttag
    27361 actcaggata agtttctgaa gcgttaacgt ggatacctag atgagctcca tatttcttag
    27421 ccttctcaat tagggtcttg aagtcttcga caccaccgat acgcttacca atatcagcat
    27481 agttcaagtg accagagtca tggccttcgc taccatatcc tttaaggaga acaccttgcc
    27541 caagaccatc tgtgtggaga ttgattttct tgataccatc caaggtcata aggaatgggt
    27601 tttgtgcttg agaaccaaag ttcatcgcga tacggtaagc tgtgatatcc ttaacttttt
    27661 cccaaccttg agggttgttc ataatgctac gataagcaat ggcaccatct tgccaatcaa
    27721 ctttcttgtc tgcattggca tcttcagtga taacaacctt agcacttgga agttccttcg
    27781 tgtattctgg gaaaacaatg cccttataag ctttttccca ttgccattca gagctgtgga
    27841 ttcctacata gttggcattt ccgactgttt ctttataagc tgtcaaacga gtccagtcat
    27901 tcgaaccacc accatagcta ttttgagagt tactccaaac accagcagca agcttatctg
    27961 tagaaacaaa tccatacatg taacccttag ccaaatcctt cattggattg gttacatcga
    28021 tatgatcatc tccgctgaca tgcgtattgt ttgacatggt tgccccatca aacttagcac
    28081 cagtttgatc actagaaaca gagactaaag cattgccgag gaaactaata gaagaaagta
    28141 gttttctttc gtcatcaatc ttttgacctg gagtgacttg attgtggttg acaatcttgg
    28201 tcacatcaaa gtgcaattga ttgtccacaa cttgcaagcg tactgtcatt tccgcattga
    28261 ttaagtgagc atcatcgcga agcttcatca agtactctgc tgttgtctca ttgatttttt
    28321 tataagtgac ttcaggggtg attcggtggt tattgataaa gacttggttg aattgttgca
    28381 cctgtcctgg caaagtatgt ccattcaagg tgtattcctt gacacgaggg aaggcttggt
    28441 caattactgc cttaagtacc ttagactgga tcgtatcata agtcaccttt ctatcgtcaa
    28501 caaccggacc tgtttctttc tgggtagggg tatcctctgt ttttaccctc tcttggttat
    28561 ccgttttaac gctaacaact gttcgctcat cgtcataaga gcccgccttg agaagaatct
    28621 tcttctcatt tttaagatgg tcattgaccg cagctggtag agtcactgtg tcaaagagat
    28681 tgacatcgtt attgctggca tttagctgac cgtctgactt gagagtgata gagagacggt
    28741 ttgttgatcc tgtttcagga gcagcaacac gactacctct ataccaagtg ctagttgttg
    28801 gagatttata ctcccagaac cagccatcct tgtcataacc gacaaaaaca ttattattgg
    28861 tatctttaaa tttcaagaag acaccaaagc gtgatttgcc cttttcagaa tcttctttga
    28921 aggttaaatc aacagttgca tttccattgg catcaacggt caagcccttc ttttcaaaca
    28981 gagctggttt acctgcgtta tcattttgag cagttgagga tagttggttg tagcggacac
    29041 ctttttcttc tcggatagtg actgttccct gttgttcttt tttctctacc gtttgccatt
    29101 caggggttac cgtcttaggc gtttcaggtt tagcagctgc aggtttatcc tcttttggtt
    29161 tttcttcagc cggtttttct ttttcaagtg ctaatagttc ttcttctgtc ttaggtccat
    29221 ctgtaacttc tggagaacct tggtcttctc ccaccttagg cgcttcaaaa tcacgcccat
    29281 cattctcttt tgctgttgca agagcagtag ctaaatcagc tggtaagttc gccgtggaac
    29341 cagactgcac gctatctgca agaaccggac ttgtcccaaa gaatgcagct ccaatcataa
    29401 cagaagcaac acctaatgaa aatttccgaa tactatattt acaacgtttt tcaaataatc
    29461 ctttattcat aaacttctaa atcctttcat ttttgagcaa gcgtttacat tttcttttta
    29521 aaaaatactc cttcctctga aagtgcaatg cttaaccctg at
//
LOCUS       DBERMR010000027        24000 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.027, whole genome shotgun sequence.
ACCESSION   DBERMR010000027 DBERMR010000000
VERSION     DBERMR010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 24000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24000
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.027"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(303..2192)
                     /locus_tag="ACL8A1_001251"
     CDS             complement(303..2192)
                     /locus_tag="ACL8A1_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HGS1202020.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIHLDNLPAKRGVELHAYWPRTNFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSSFEKDCLNLAFSGKKEETLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGGEKALQVGMG
                     ALTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDQAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSTAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            2330..3895
                     /locus_tag="ACL8A1_001252"
     CDS             2330..3895
                     /locus_tag="ACL8A1_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HGS1202021.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            3895..4659
                     /locus_tag="ACL8A1_001253"
     CDS             3895..4659
                     /locus_tag="ACL8A1_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGS1202022.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IIAQTTVLKRERTEAEEIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLILNENQRAN"
     gene            complement(4859..5074)
                     /locus_tag="ACL8A1_001254"
     CDS             complement(4859..5074)
                     /locus_tag="ACL8A1_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202023.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
     gene            complement(5943..7193)
                     /gene="ilvA"
                     /locus_tag="ACL8A1_001255"
     CDS             complement(5943..7193)
                     /gene="ilvA"
                     /locus_tag="ACL8A1_001255"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HGS1202024.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(7243..7599)
                     /locus_tag="ACL8A1_001256"
     CDS             complement(7243..7599)
                     /locus_tag="ACL8A1_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HGS1202025.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIH
                     MAHKYHESVTEVFGDE"
     gene            complement(7837..8073)
                     /locus_tag="ACL8A1_001257"
     CDS             complement(7837..8073)
                     /locus_tag="ACL8A1_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202026.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMNFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKH"
     gene            complement(8180..9202)
                     /gene="ilvC"
                     /locus_tag="ACL8A1_001258"
     CDS             complement(8180..9202)
                     /gene="ilvC"
                     /locus_tag="ACL8A1_001258"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HGS1202027.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(9268..9744)
                     /gene="ilvN"
                     /locus_tag="ACL8A1_001259"
     CDS             complement(9268..9744)
                     /gene="ilvN"
                     /locus_tag="ACL8A1_001259"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HGS1202028.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(9737..11437)
                     /locus_tag="ACL8A1_001260"
     CDS             complement(9737..11437)
                     /locus_tag="ACL8A1_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HGS1202029.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDALPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            complement(11638..13305)
                     /locus_tag="ACL8A1_001261"
     CDS             complement(11638..13305)
                     /locus_tag="ACL8A1_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HGS1202030.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(13308..13673)
                     /locus_tag="ACL8A1_001262"
     CDS             complement(13308..13673)
                     /locus_tag="ACL8A1_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HGS1202031.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(13830..14018)
                     /gene="rpmB"
                     /locus_tag="ACL8A1_001263"
     CDS             complement(13830..14018)
                     /gene="rpmB"
                     /locus_tag="ACL8A1_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HGS1202032.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(14134..14310)
                     /locus_tag="ACL8A1_001264"
     CDS             complement(14134..14310)
                     /locus_tag="ACL8A1_001264"
                     /inference="COORDINATES: protein motif:HMM:NF013652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGS1202033.1"
                     /translation="MDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     NGMWRAENVTQDLI"
     gene            complement(14421..14618)
                     /locus_tag="ACL8A1_001265"
     CDS             complement(14421..14618)
                     /locus_tag="ACL8A1_001265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202034.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCIHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNV"
     gene            complement(15046..16590)
                     /locus_tag="ACL8A1_001266"
     CDS             complement(15046..16590)
                     /locus_tag="ACL8A1_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HGS1202035.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLETFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMNPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            16803..17105
                     /gene="gatC"
                     /locus_tag="ACL8A1_001267"
     CDS             16803..17105
                     /gene="gatC"
                     /locus_tag="ACL8A1_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HGS1202036.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVVEEGTDRDRLFKNVPEKDNYYIKVPAILDDGGDA"
     gene            17105..18571
                     /gene="gatA"
                     /locus_tag="ACL8A1_001268"
     CDS             17105..18571
                     /gene="gatA"
                     /locus_tag="ACL8A1_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HGS1202037.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            18571..20013
                     /gene="gatB"
                     /locus_tag="ACL8A1_001269"
     CDS             18571..20013
                     /gene="gatB"
                     /locus_tag="ACL8A1_001269"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HGS1202038.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYK
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            20137..21180
                     /locus_tag="ACL8A1_001270"
     CDS             20137..21180
                     /locus_tag="ACL8A1_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HGS1202039.1"
                     /translation="MATMKAARWHAAKDVRIQEIEVPEVLPHQVKVAVKFTGICGTDL
                     HEFLDGPIFIPTDEHVYSGQKAPVTLGHEFSGEIIEVGSDVTRVKVGDRVAVEPILAK
                     NNLVGDYNLDPNLNFVGLAADGGFAKYCVLDGDLVHVIPDSLSYEQAALTEPAAVAVY
                     AVRQSSLKAGDTAVVFGLGPIGLLIVEALRAAGASKIYAVELSPERQAKAEELGAIVV
                     RPEEGETAVEAIHRLTNGGVDVSYEVTGVPVVLGQALAAVHKAGECMVVSIWEREANI
                     NPNEFAIQEKTLKGIIAYRHIFPKVLELMEQGYFSAEKLVTKKIKLENIVEEGFIELT
                     QDKSQIKILVEPE"
     gene            complement(21205..21435)
                     /locus_tag="ACL8A1_001271"
     CDS             complement(21205..21435)
                     /locus_tag="ACL8A1_001271"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202040.1"
                     /translation="MVNVKEYPLYFFSYSMEIKEQTRKLAAGCSKHCFEVVDRTDEVS
                     NHIYGNVTLTWFEEIFEEYYISKGRSKFTPTC"
     gene            21558..22118
                     /gene="efp"
                     /locus_tag="ACL8A1_001272"
     CDS             21558..22118
                     /gene="efp"
                     /locus_tag="ACL8A1_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HGS1202041.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            22140..22529
                     /locus_tag="ACL8A1_001273"
     CDS             22140..22529
                     /locus_tag="ACL8A1_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HGS1202042.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            22522..22944
                     /gene="nusB"
                     /locus_tag="ACL8A1_001274"
     CDS             22522..22944
                     /gene="nusB"
                     /locus_tag="ACL8A1_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HGS1202043.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREYT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(22979..24000)
                     /locus_tag="ACL8A1_001275"
                     /pseudo
     CDS             complement(22979..24000)
                     /locus_tag="ACL8A1_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT         7122 a         5349 c         4445 g         7084 t
ORIGIN      
        1 aaggaaaaca ttacaatgta gccatcatcc accttgcaaa aaaacttatc cgaaccctgt
       61 tttatatcct aaaaaagagc tgccatttga cgaacaaaaa gtgatgtaat ttctgaaatt
      121 aacacaggat tacctttgaa aaaatggact ttttcaaggg ctttttgccg tgctctaaaa
      181 tcatcgccaa atcatcaaat tgaaataaga aatagtttag ataaaactat tgacttttct
      241 tatagtttgt cttttttcta tcttccatta tatcatactt tttcagtcta tggtagctct
      301 ctttaaaagg caccgatact gccgccacct ccgcctccag agaagccacc accagaactt
      361 ccacttccag aagatacaga gtaggtgctt gctgtatttg cgacactagc ataatggctc
      421 atttgtgctg ttgaatgata aaacatactg tgccagccat aagctacata gagattgata
      481 tctggatttt ccacttgaat ctgatgaacc ttcatcaaat gactaacctt gtccgcatag
      541 ccaaataagg tcgcatagac caagaggcga ttccagacca caatactttc cagttcagcc
      601 tgatccaatc gtgcaatctc acgcaacata ttttcaaaac tggtccagag atagtagacc
      661 tcagctcccg cttcatttag aacaccatca cgattatcta gtcgaagctt ccaataatag
      721 aaaacagaca aaactaaccc tagaaaacca agtattggca aagggaggta aagatagcca
      781 tgaacgtcta aactgtacaa gaacaaacca aatccgataa ataggggcag gatagtcaag
      841 gcacccatac ccacttgcaa ggccttttcc ccaccagtta aaggacgata ataatctggg
      901 agcccccaga aggaaactcg ttttctcact ccttcttgca tctggttcaa tacctcttca
      961 aaagaagatt tgagttgaag ccctcttgct tgaatccgtt tttcatcaga aactttggct
     1021 ctacgataaa gactatcaga taccttgtaa tccgcaaaca aattggaaag agtttcttct
     1081 tttttacctg aaaaagctag atttaggcag tctttctcaa agcttgacaa accatcttct
     1141 tttactagcc tcaaaccaac tgcatctcct tctgaaatga tagagacatt cccacggtct
     1201 atcacatcta gcaaggtagc ttgaataagt tgatcaaagg tgaattttcc agctcccttg
     1261 accaagggac tcacttcctc caaggaggtc gagtagactg cttctgataa aaccataggc
     1321 tctaattcca ttggtggttc atagagacga tgatttttgg catatttgac tgaaggagtg
     1381 gtctttcttc tataaataaa atagaagcag acactcaata acaaggagat agaaagtatc
     1441 gaagggagga cccaagtaac gagttgttta ctctgatctt tttctctaac aatcgagtct
     1501 tctatcttat taaactcttc taaacgattc cctttcaatc cctgatccct agcgctagca
     1561 aaattggtcc gaggccaata ggcatgcaac tcaactccac gcttagccgg aagattgtct
     1621 aaatggatag tataatcaag gttactcttt tcaatcgttc cctctctaaa aagtttccct
     1681 gtatggaaaa agagtttttc agcccccttg tctcccctta catgaaattc aaacttttca
     1741 atagactctg aactatctgt cagaggttgc caatttaatt cagcgatatc atcataaagg
     1801 aaaagtaaat tttttaagtt ccagacgagg tcaacttcaa ctatgtcgcc ctcctgacct
     1861 ggattataga ctctcacagt ataaccatcc gcttcttctg ttacttcgct agtcacatct
     1921 gctagttctg caccgttttt cgcggcctga acctttggat gagggtcaat gtcaaacccg
     1981 ctaggcatct taccagcacg tccaagtccc acgatttggc ccttaaagtc ctcctcaaac
     2041 tggtaaacta tcttctgtct aaactctgcc gtattatctg catgaatata caaatcccct
     2101 tgataagagt ttatcttgaa atcaatggca aaaacagaga gtggaagaag gcaaaacaag
     2161 cctaacacca gtaagaaaaa agtttttttc atcaactaag cccccttttt atctttctta
     2221 tcattatagc attttttcgt aagtaaaggc ttacttatgt tgaaaaatta cgcatttttc
     2281 gataaaatag gagacagtta ttttttaata gatcagaata ggagaaatca tgagaaaaat
     2341 atacttatct attttcacaa gtctcttgct gatgctagga cttgtcaatg ttgctcaagc
     2401 cgatgaatat ttacgcatcg ggatggaagc agcatatgct ccctttaact ggacccagga
     2461 tgatgatagc aacggagctg tcaaaatcga tgggaccaat cagtatgcca acggatacga
     2521 tgttcaaatc gccaagaaaa tcgctaagga cttaggtaaa gaacctttgg ttgttaaaac
     2581 caagtgggaa ggtctagtcc ctgcccttac ttctggtaag attgacatga ttatcgcagg
     2641 tatgagtcca actgcagaac gcaaacaaga aattgccttt tcgagcagtt actacactag
     2701 cgaaccagtt ttgcttgtca aaaaagattc tgcctacgca agtgctaaat ctttggatga
     2761 ctttaacggt gcaaaaatca cttctcaaca aggggtctac ctctacgact tgattgcaca
     2821 aatcccaggt gctaaaaaag aaacagccat gggagacttc gctcaaatgc gacaagctct
     2881 tgaggctggt gtcattgatg cttatgtttc cgaacgtcca gaagcactga ctgctgaagc
     2941 tgcgaactct aagttcaaga tgattcaagt agaacctggt ttcaaaactg gggaagaaga
     3001 tacagctatc gctatcgggc ttcgtaaaaa tgacaatcgt attagccaaa tcaatgccag
     3061 cattgaaacc atttcaaagg acgaccaagt tgccctgatg gatcgtatga tcaaggaaca
     3121 acctgccgaa gctacaacaa ctgaagagac tagcagtagt ttctttagcc aagtcgctaa
     3181 aattctttct gaaaactggc aacaactctt gcgtggtgct ggtatcactc ttttaatctc
     3241 tatcgtcgga accatcatag gtctcattat tggacttgcc attggtgtct tccgtactgc
     3301 tcctctctct gaaaacaaag ccatttatgg ccttcaaaaa ctagtcggct gggttctcaa
     3361 tgtctacatt gaaattttcc gtggtacgcc aatgattgtt caatcgatgg ttatctacta
     3421 tggaactgcc caagctttcg ggatcaacct tgaccgtaca ctggctgcta tcttcatcgt
     3481 ttcaatcaat accggtgcct acatgactga aatcgtccgt ggtggtatcc tagcagttga
     3541 caagggacaa tttgaagctg cgactgctct tggtatgacc cataaccaga ccatgcgtaa
     3601 gattgtccta cctcaggtag tccgtaacat cctacctgca actggtaatg aatttgtcat
     3661 caatatcaaa gatacatctg tattgaacgt tatctctgtt gtcgaacttt atttctcagg
     3721 aaataccgtg gcaacacaaa cctatcaata cttccagaca tttacaatca tcgccgtgat
     3781 ttactttgtc ctcaccttca ccgtaacacg tatcctacgc tttatcgagc gcagaatgga
     3841 catggatacc tatactacag gtgctaacca aatgcaaacg gaggatttga aataatgaca
     3901 caagcaatcc ttgaaattaa acacctcaaa aaatcctatg gacaaaacga agtgctaaaa
     3961 gacatttctc tcactgtcca caagggagaa gttatctcta tcatcggaag ctctggaagt
     4021 gggaaatcga ccttcctacg ctccattaac ctacttgaaa cgccaactga tggacaaatc
     4081 ctttatcatg gacaaaacgt cctggaaaaa ggctatgacc tcacgcaata ccgtgaaaag
     4141 ttaggaatgg ttttccaatc ctttaacctc tttgaaaatc tcaacgttct tgaaaacaca
     4201 atcatcgctc agacaactgt ccttaaacgc gaacgcacag aagctgaaga aattgccaaa
     4261 gagaacctgg aaaaggtcgg catgggagaa cgctactggc aagcgaaacc caaacaactc
     4321 tcaggtggtc aaaaacaacg tgtggccatc gctcgtgccc tttcaatgaa tcctgacgcc
     4381 attctctttg atgaaccaac atcagctctc gatccagaaa tggttggaga ggtcctcaaa
     4441 atcatgcagg acctggctca ggaaggcttg accatgattg tcgtaaccca tgaaatggaa
     4501 tttgcccgtg atgtctctca ccgtgttatc tttatggata agggtgtgat tgctgaagaa
     4561 ggtaaaccag aagacctctt cactaatcct aaagaagacc gtacaaaaga attccttcaa
     4621 cgttatctca tactcaatga aaatcaaaga gcaaactagg aagctagctg caggttgctc
     4681 aaaacactgt tttgaggttg cagatggaag ctgacgtggt ttgaagagat tttcgaagag
     4741 tatcaaataa aaagaaaagg ctgcatcaac cgtgcagtct ttttgctgtc ctcaaaatga
     4801 aaaggcagat tctctatctc tactacttga aggtcaagaa tccgctgtta tccaaagatt
     4861 aatcttcaaa tttttcataa tttttttcat agaaatcaat taggcctagg gctgtttcaa
     4921 atgaaatatt ttttactttt gcacgaccct gcgccagagc aatgatagac atttcacgcg
     4981 cattcgtttc tttagagata cggtagcctg tgattttctt atcacgaacc cagccaacaa
     5041 ctgattctac tttttcaaag tttgacttag ccatgttttt ctcctatttt cttctttata
     5101 atatttaatt gtatacgttt ttactacttt tgtcaataaa aaaaacatat attcaacgaa
     5161 aataattagt tcaaaatttt cttacttcct ttttaaatcc gataatatat aggttttgct
     5221 agcctctaca ccataaatat ttgctaataa aaatactgta ttattaccct cttaaggtgc
     5281 gaaattattc aagttcttaa aaaatgcttc tctatttccg taagatagct tatttcttcg
     5341 atttaagaac tgaagatgat agttctaacc tttagaaatc caagccttca ctagctcaaa
     5401 aactcctatc tttcttcctc aataaatcat ctgagagata ggtattcctt atatccttca
     5461 aattcatcac tcctttggat attgaggtgc tagcctagaa gcagactccc gattatctaa
     5521 accatcctta ataaagatga caagacagat aagctcagtt aattacttat atatcttttt
     5581 aatatggtat atttgtcaac aaaaaaatca aatcatacct tatactcaat gaaaatcaaa
     5641 gagcaaacta ggaagctagc cgcaagttgc tcaaaacact gttttgaggt tgcagataga
     5701 gctaacgtgg tttgaagaga ttttcgaaga gtattaaaga cagtgtgact atgcttgtca
     5761 atagatagga aatcaaaatg aaggatggaa atctgagcca aacatcataa gtgctataat
     5821 gaacagaagt aagaattgca cgataagttt cctaaaacca taggaatcgt cccctttctt
     5881 catcttcatt atagcacata tacatcagtt gtacaaataa tattatattt ttttattagt
     5941 cctcagacaa gcatattata aagcgtttca ttaccattta agttaatata agctggatca
     6001 aaaccttcca ttctacggat caaacctgca taatcatgct tatctgctaa agcgatccca
     6061 attaatactg ggcctgttcc cttgctagct cgtttgatat actcaaaacg tgtgatatca
     6121 tcatttggcc ccaggatatc atttacaaac tcacgcaaag ctcctggacg ttgtgggaaa
     6181 ttgaccacaa agtaatgttt gataccatca taaatcaagg cacgctcttc catttctggc
     6241 atacggttga tatcattatt tcctccagaa atgatacaac aaatggtttt ccccttaata
     6301 tattcagcta aaacctctaa agaggcgata ctagccgctc cagcaggttc tgcgactatc
     6361 ccttgcttag agtaaaggtc aatcaaggtt tcagaaatca atccctcatc gacacctacc
     6421 aaagtttgaa tatgttgacg agttgcttca taggtcaact gacctacctt ttgcacagca
     6481 atcccatccg caaatttatc aatttccttg agtttgactg gacctccagc ctcaaaggca
     6541 gctttcatgg aacgcgctcc attcgcctct actccgataa cctcaatctc tggacttgtt
     6601 tccttgatat aggtagaaac cccggcaatg agaccgccac caccaacagg aaccaagaca
     6661 gcatcaaaat caatcgattc ttttcgagct tcttctaaaa tctcataagc aactgttcct
     6721 tgacctgctt gaacatgagc atcatcaaaa ggatcaataa aggtacgatt ttcagagact
     6781 gtaaattctt gagctgcttt ggctgaggca tcaaaggtat ctccaactag tttaatagtt
     6841 acaaaatccc caccaaaaaa gcgaacctga ccaatctttt gttgtggcgt agtaataggc
     6901 ataaagatag tagcaggaat tttcatttca ttacaagtat aggctactcc ctgcgcatga
     6961 tttcccgcag aagcgcagac taccccacgt tcacgttctt ccttgctgag ctgggaaatg
     7021 gcataatagg caccacgaat tttaaaggag cgaacacgct gggcattttc ttttttcaaa
     7081 taaatcttag caccatactt ctccgataaa taatgatcgt aatccagtgg agtattcaca
     7141 accacaccgt tcaagacctt gtgagccttg attatatctt ttgaacttaa catataaaac
     7201 ctctttttct ataaaaaaca gtcactaatg actgtttttt agttattcat ctccaaaaac
     7261 ttcagtaaca ctttcatggt atttatgagc catatgaatc gaaaactctt taggaagttc
     7321 ataacgattt gtggaaatcc aatcatataa ccattctacc aaactttctt cactatcatt
     7381 acgatggaag ataaaatagt ttaaaatttc tttatattct acatcattca aatccaacgt
     7441 gtctacaaat tgactacgag gaacaaaatt agaatctaat tttcttagtt gatctgtcaa
     7501 ttctcccaat tgtaaatcca actgaaaaaa tagtttttgc agttgcctat cagtttcatc
     7561 acatcctgtc ttgatagaaa acaaattggt aatattcata tgatttcaac ccctaaataa
     7621 gtacatctaa tatcccacga atagctgaag ctaaagtttt cgcagtccag tcatttaaac
     7681 ccgattcttt caatccagat gcaataattt gctctatttg atataaaccg ccatctatcg
     7741 tctcatttat tctgcgaaga ggaccttcaa tataggataa agcttctccg aatctaacag
     7801 ctgatttagg tcctaatgat ttaccaacta tatttgttaa tgttttttat ttctcaaaat
     7861 ccatttcgct atataacgag cagatttagt cgcaccagct ctctcaaacg gagaatgaat
     7921 ttcaagacct tcttctctag ctatttgtgc caatttgaag aattgttcaa agttcatatg
     7981 caacctacca tcttcaaaat tagcatcatc ataaatgtta attacagcat tttcatcata
     8041 cgaccaatct gctttatttt ggtctgctag cactattacc gacggtgtaa ttacattata
     8101 caactagaac caataataac gatgcaattt ttctcataat ctttttcata aaatcatctc
     8161 cagcgctata tatttctaat tagttataga ttttgaatgc atcatcgtcg tttttaccaa
     8221 cgaatggcat tgctttacgc aattctgcac caactttttc aatttcaagg ttagctgctt
     8281 gttcacggta agcagtcaat tttggacgtc cagctttata gtcatttaca aagtcatttg
     8341 caaatttacc attttggatg tctgccaaga cagccttcat attttcttta acttgttcag
     8401 tgattacacg tggacctgat acatagtcac cgtattcagc agtgtttgaa atagattgac
     8461 gcattttctt gaatccacct tcgtagatca agtcaacgat caatttcatt tcgtgaagaa
     8521 cttcaaagta agccaattct ggagcgtaac ctgcttctgt caagacttcg aaacctgctt
     8581 cgataagggc agtcaaacca ccacaaagta cagcttgttc accaaacaaa tcttcttcag
     8641 tttcttcttt gtaagttgtt tcaagaagac ctacacgagc cgctccaaca cctttacacc
     8701 agtccatagc aatgttttta gcatttcctg ttgcatcttg gtatactgca taaagagctg
     8761 gaacaccaaa tccttcttcg taagtacgac gtaccaagtg tcctggtcct ttaggagcac
     8821 acatgaagac atctacatcc gcaggaactt tgataaattc aaagtggatg ttgaaaccat
     8881 gggcaaatcc aactgcgttt ccagcttcca agtttggagc gatttctgct tcgtacaatt
     8941 cttgttgaat ttcgtctggc gccaagatca tgataacatc agccaactta gtagcttctg
     9001 ctactgtgta agtatcaaat ccatcttctt ttgctttatc aaaagattta cctggacgta
     9061 caccgataat aacgtcacga cctgaatcac gcaagttttg agcatgcgca tgcccttgtg
     9121 aaccataacc gataacggcg atttttttac cgtcaagtgc tgctacttta acatcttttt
     9181 catattccat ttgaactgtc atagcttttc tctcttttct attatttatt gccttttaag
     9241 ctggtttaac aaatttaagt tggattttta atcgcgggta aatccagttg cacccgttcg
     9301 agcaatattg cgaataccgt atgggcgaat gactcgcaat agggcttcgc tcttttctgc
     9361 atttcccgtc atctgaatgg taatcgagct tggcgctacg tctaccactg ttgcacggaa
     9421 aggttgaata atcgctaaaa tctcagctct cttctcagct ggcgctgaca tcttaaccaa
     9481 aatcacctcg cgctccaaat gaggcttgtc tgtaatatct cgaatgcgaa tcacatcaat
     9541 ctgacgattg agctgtttga tgatttgctc cacttcatca tgagaagcaa catcaataat
     9601 aatagtgata cgcgatacat tcggatcttc tgttgctcca acagagatgc tttcaatatt
     9661 aacctgacga cgagatagga cacctgtaaa gcgattgagg actcctgaac gattttgtag
     9721 ttttgctgtt aacattctac gcatggaact gcacccccaa catctcatga ttactcttac
     9781 cagccggtac cattggtaac acctgttcct tacgagaaat atctacctca attagcatag
     9841 gaacatcctc agtgatgact tcaaggtctt gagccaaggt ctcaggattg tcaaacttat
     9901 agtttttaat accataagcc tgcgccatca attggaaatc aggaagggca tcaaagaccg
     9961 actctgatgt tctgccttca tagaaggatt cctgccactg gcgaaccatt ccaagtgaat
    10021 gattgttcag cataaccacc ttgattggca ccttgtaaat attcaaaata gccaactcct
    10081 ggttggtcat ttggaaacca ccatccccaa caaacaagac tacttcctta tctgggttag
    10141 caattttagc accgattgct gctggaattc caaagcccat tgttcccaaa ccacctgaag
    10201 tcactaactg acgctcattt tggtagggat aatactgagc tgtccacatt tggtgttgac
    10261 caacgtctgt taccacaatg gcatctccat tcgtcaattc accaattcgt tcaataactg
    10321 cttgcggttg aaccacacgc tctttcttgt cataagaacg aacacgattc ttgtctttag
    10381 tgactttctc aatccacttt tcagtattgt tgtgaactgt tggttctgct agcaacattt
    10441 gcaaggcctt cttagcatct ccaactacag gaatgtctgc actgataatc ttgccaatct
    10501 cagctgggtc aatatcaatg tgggcaacct tagcattctt agcgaaagtc ttaggattcc
    10561 ccgtcaaacg gtcatcgaaa cgagaaccaa tactaatcat aaagtccgct tccgtcatgg
    10621 caatatttgc tgcgaatgac ccgtgcatgc ctcccattcc aagaaagagt gggtgactcg
    10681 ttgcaatcgt tccttgtccc aaaagactgg ttaccactgg aatttgatag cgttctgcaa
    10741 attcatttag ttccgtagca gcctcagcat aactaattcc accaccagct aacaagactg
    10801 gctttttagc cttggacaat tgcttcaaga ttttcttgat ttgcatatca ttcggctcaa
    10861 gagtcggctg ataacttggt aaattcactt ctggtgaata aatgaagtct gtttctaaag
    10921 cagatatgtc ttttggtagg tcaattacaa ctggccctgg acggcctgta gttgcgatat
    10981 ggacagcttc cgtaatgata cgcggaatat cagctgtctc acgaacttgg taattgtact
    11041 tagtgattgg catggtaatt cccacgatgt ctgcctcctg aaaggcatcc ttcccaatcc
    11101 ctgctcgcgc cacctgacct gtaaagacca aaaggggaac gctatcgctc atggcatccg
    11161 caatccctgt aatggcattt gttgctcctg gtccactagt aacgacggca acacccaact
    11221 ttccagttga tttggcataa ccttcagctt catgcaaaca accttgctca tggcgcccta
    11281 gaatgtggcg aatgccttta aaattatata tcgcatcata aaaaggcaaa accgcaccac
    11341 caggataacc aaagatggta tcaactccta aatcacgaag tgtttccaaa actaggtccg
    11401 accccgtctt aggagattct aaactgattt tctccattgt tcccctttct ttcctcttaa
    11461 aaataacttg ttactatcat accatttttt caaaattttt caagacaaaa gaagaaattt
    11521 tctgaatttt ctattttaac gtttatttat gaatgttatt tttgttttta ttaaaaagat
    11581 accgatttca tttactaaaa aagaaaaaag aactgatttc tcagtccttc attaatctta
    11641 ttccacacta aataggtatg ggtaaacagg ttgttgacct tggtgaatct cgacttcaac
    11701 gtcttcgaat tcttctacga tttcttgagc gatttcattg gcaagttctt cgcttccgtc
    11761 ttcaccgaca tagaaggtta cgatttcact gtcttcatcc aacatatgtt tcaaggtttc
    11821 agtcaatgtt tggtgcatat cagggtttga cacaagaatt tttccatcca ccatacctag
    11881 attatcgttt tcatggattt ctaagccatc gatcgttgta tcacgcacgg ctgttgtgac
    11941 gcttccgcta acgacatcgc taagagcagc tgtcatacgc tcttggtttt cttcaatgga
    12001 cttgcttgga tcaaaggcaa gaagacttgt cataccttga ggaagagtgc gagcctctac
    12061 cactactgct ggttgctcca aaacttctgc cgcagattga gctgccatga agatgttctt
    12121 gttgtttggc aagaagatga tgttacgggc attgacctgt tcaacagcct tgataaagtc
    12181 ttctgttgaa gggttcatgg tttgaccgcc ttcgataaca taatccacgc cttgagaaca
    12241 gaagatatct gctagacctt taccagctac cacagcaatc aaagcatact ctttttcttc
    12301 agccgacttg ataacttgag tagcttcttt ctcaacctgt gcttcgtgtt ggttacgcat
    12361 attgtcaact tttaccttga ccaagctacc atatttgaga ccttcttgca taacaagtcc
    12421 tggatcttct gtatgaacat ggactttgac aatttcatca tcgttaacaa cgaggagaga
    12481 atccccaagc tcatccaagt agttacggaa ttcatcgtag tcaaaatctt tagcataggt
    12541 tggaccttgc ttaagagcta ccatgatttc agtacagtaa ccaaacgtga tgtcctcagt
    12601 cgctacgtga ccagctacag acttatgatg ctctacattg atcatctcac tcatgttggc
    12661 aggagtcgct acaaagtcct cagatgcaat atattcgcca gtaagggctg aaaggaaacc
    12721 ttcgtagatg aagaccaatc cttgaccacc tgagtccaca acgccaactt ctttcaatac
    12781 tggaagcatg tctggtgttt tagctagagc tgttttagca ccttccaagg ctgcgcgcat
    12841 gacttcaaca gtatcatctg tttgctcagc ttttttctta gcaccgatag cagctccacg
    12901 agaaactgtc aaaatcgttc cttcaacagg tttcatcact gccttatagg caacttccac
    12961 acctgattgg aaggccaggg ccaagtcttg acctgttaac tcgtctttat ccttgatagc
    13021 ttgggaaaat ccacggaaaa gctgagacgt aatcactcct gagttcccac gcgcacccat
    13081 caaaagccct ttggcaagaa tgctcgctac ttctccaact gtagaagctg gcttgtctgc
    13141 aacttcttta gcaccatttt caatggtcat tcccatattt gtcccagtat ctccatctgg
    13201 aactggaaag acgtttaatg aattgacata ttcagcttgc ttattcaagc gagttgatgc
    13261 agcctgaacc atttcttgaa ataagctagt agtaattttt gacacggtta ttctcctaca
    13321 actttgatat tttgaatgta gacatttaca gtctgagcag taattccaag ctggttttcc
    13381 aagctaaagg caacacgctc ttgaatgttt tttgacactt cactaatctt tgttccgtag
    13441 cttaacacgg tatatacatc aactgcaata ctgccatctt cggctgcctt tacgacgaca
    13501 cctttagaat aattttcctt acctagcagg gcttggaaat tatctttgag ggcattttta
    13561 ctagccatac cgaccacacc aaaaatctca gttgctgctc cacctacgac ggttgcaatc
    13621 acttcatctg ttagttcgat ttgaccatct tttgtattaa tttttacagt catccttttt
    13681 acctcaacta gttgatactc tattttatca tatttcagcc caagtgtaaa agcagaatga
    13741 tgtatcagcg gatatttact ctatttttca aacgatgtta tacccacaat aaaagaaaaa
    13801 agaccctaag gtctcctttg cttttattat taaacgcgtt caactttacc tgatttcaaa
    13861 gcacgagctg aagcccaaac ttttttaggt ttaccatcga taagaacagt aactttttga
    13921 aggtttggtt ttacggcacg ttttgtttgg ttcatcgcgt gtgaacggtt gtttcctgat
    13981 acagtcttac gacctgtaaa gtaacatact ttagccattg tgttttcctc ctattagatc
    14041 taatatagcg gatgtgctag caccacatac cgtactatgt tatcacactt tcttgttttt
    14101 tgcaagggaa ttggaagatt ttttatttgt gtcttaaatc aggtcttgcg tgacattttc
    14161 tgctctccac atgccattgt tgattaacag aacaccagaa ttaaaattat gtgtataaaa
    14221 atcatctcta actgcagcta agggtatagc cgtcaagtcc aaatcccaca gctcatctat
    14281 cgattttctt acaacaatat ctgaatccaa atacagtaca cgagactcgc ttacatactt
    14341 tggaataaaa tacctaaaaa agccgcatat gaaagtccct caaaggggag acgataacct
    14401 ttcagaatac tactgtcaat ctaaacattc acaatctcac tattcaaagt ctctaatctt
    14461 ttttccatca attggaacca ttctcgcgga aggtcatcat taaaaacata aaacttaaga
    14521 ttataatgat gaatacaaag agattttatt gttgtttcaa ctttatccat ataagcatta
    14581 tctgcaccta agacaatcgc ttttttctct tctttcactc tttatcttat ttctttttat
    14641 tcccatcata tgtttctacg taaccattat tttcgcctat tcgttcgtaa aaccatacca
    14701 gtggagattt tagatgaagt cccattacgg tttacaattt ttacattacg acacggagtt
    14761 ttacaaatcg atttcatttg ccaaacgtag ttagtaaggc atttagctag gctacctact
    14821 ttctgctacg acggaaaaat ccacaaatca ataccattcg tggatttttc ctagtgaagc
    14881 ggttaggtaa gccgccatag cggttaccga ttattagatt acgacacgga gtttggcaaa
    14941 tcgattctat ttgccaaacg tagttagtga ggcagttagc tagttcgcca aatagcgact
    15001 agcgtccaac aatttggaac tttagttcca attgttggta ctgagtcaca tcttctcctc
    15061 taactctacg tctggatact tgtccgcaaa ccagcggagg gcaaagtcat tttcaaagag
    15121 aaagactggt tggtcaaaac ggtctttggc taagatattg cgacttgacg acatccgttc
    15181 atccaagtcc tcaggcttga tccaacgaac ggtcttttta cccattgggt tcataactac
    15241 ttccgcattg tactcgcctt ccatgcggtg tttaaagact tcaaactgga gttgacctac
    15301 agcgcctagc atgtactcac ctgtttggta attcttataa agctgaacgg ctccttcttg
    15361 caccaattgc tcaatcccct tgtggaaaga tttttgcttc ataacattct tagcagaaac
    15421 tttcatgaaa atttcaggag taaaagttgg cagtggttca aattcaaact tgtttttacc
    15481 aactgtcaag gtatccccaa cttgataagt acctgtatcg taaaccccga taatatcacc
    15541 tgccacggca ttggtcacat tctcacgact ttccgccata aactgagtaa cattagacag
    15601 tttagccccc ttaccagtac gagggagatt aacactcatg ccgcgctcaa attcgccaga
    15661 tacgatacgg acaaaggcaa tacggtcacg gtgacgaggg tccatgttgg cttggatttt
    15721 aaagacaaag cctgagaaat ccttgtcata aggatccaca atttcaccgt ctgttttctt
    15781 gtgaccatgt ggttctggag caaacttgag gaaggtttca aggaaggtct gcacaccaaa
    15841 gtttgtcagg gctgaaccga aaaagacagg cgtcaattct ccagccagaa tagcttcctc
    15901 tgaaaactca ttcccagctt catttaaaag ctcaatgtca tccttgactt gctcgtaaaa
    15961 aggattgcta ccaaaaagtt tgtccccatc ttctaggcta gcaaaacgct catccccttt
    16021 ataaagttct aaacgttggt tatagaggtc atacaagccc tcaaaggctt tccccatccc
    16081 gataggccag ttcatagggt agctagcaat gcccaagatt tcttccaatt cttgcaagag
    16141 atccaaaggc tcacgaccgt cacggtccag cttgttcata aaggtaaaaa ctggaatgcc
    16201 acgatgtttc acaacctcaa acaatttctt ggtttgagcc tcgatcccct tggcagagtc
    16261 cacgaccatg accgcagcat ccaccgccat caaggtacga taggtatctt ctgagaagtc
    16321 ctcgtgccct ggcgtgtcta agatattcac gcgcttgccg tcgtagtcaa attgcataac
    16381 agatgaagta acagaaatcc cacgttgctt ctcgatatcc atccagtcag atttagcaaa
    16441 agtccctgtt ttcttccctt ttaccgtacc agcctcacga atctcacccc caaagtagag
    16501 taactgctca gtgatggttg ttttccccgc gtccgggtgg gagataatgg caaaggtacg
    16561 acgtttctta atttcttctt gaatattcat aagttctctt tctttgattc tctatttttc
    16621 ttgtttcaat agctgagaat gatttttaca ttggatttta ccattccttt caacactcca
    16681 ttatatcgga ttttagcatt tttttcaatt tctatttctt ttcacttccc cctcccttat
    16741 ttataggaaa atatggtaaa atagaacaga ctaaaaatca tcatttcacg aaaggatgca
    16801 agatgaaaat tacgcaagaa gaggtaacac acgttgccaa tctttcaaaa ttaagattct
    16861 ctgaagaaga aactgctgcc tttgcgacca ccttgtctaa gattgttgac atggttgaat
    16921 tgctgggcga agttgacaca actggtgtcg cacctactac gactatggct gaccgcaaga
    16981 ctgtactccg ccctgatgtg gtcgaagaag gaacagaccg tgatcgcttg tttaaaaacg
    17041 tacctgaaaa agacaactac tatatcaagg tgccagctat cctagacgat ggaggagatg
    17101 cctaatgact tttaacaata aaactattga agagttgcac aatctccttg tctctaagga
    17161 aatttctgca acagaattga cccaagcaac acttgaaaat atcaagtctc gtgaggaagc
    17221 cctcaattca tttgtcacca tcgctgagga gcaagctctt gttcaagcta aagccattga
    17281 tgaagctgga attgatgctg acaatgtcct ttcaggaatt ccacttgctg ttaaggataa
    17341 catctctaca gacggtattc tcacaactgc tgcctcaaaa atgctctaca actatgagcc
    17401 aatctttgat gcgacagctg ttgccaatgc aaaaaccaag ggcatgattg tcgttggaaa
    17461 gaccaacatg gacgaatttg ctatgggtgg ttcaggtgaa acttcacact acggagcaac
    17521 taaaaacgct tgggaccaca gcaaggttcc tggtgggtca tcaagtggtt ctgccgcagc
    17581 tgtagcctca ggacaagttc gcttgtcact tggttctgat actggtggtt ccatccgcca
    17641 acctgctgcc ttcaacggaa tcgttggtct caaaccaacc tacggaacag tttcacgttt
    17701 cggtctcatt gcctttggta gctcattaga ccagattgga ccttttgctc ctactgttaa
    17761 ggaaaatgcc ctcttgctca acgctattgc cagcgaagat gctaaagact ctacttctgc
    17821 tcctgtccgc atcgccgact ttacttcaaa aatcggccaa gacatcaagg gtatgaaaat
    17881 cgctttgcct aaggaatacc tcggtgaagg aattaaccca gaggttaagg aaaccattct
    17941 aaatgccgct aaacactttg aaaaattggg tgctattgtc gaagaagtca gccttcctca
    18001 ctctaaatac ggagttgccg tatactacat catcgcttca tcagaagctt catcaaactt
    18061 gcaacgtttt gacggtatcc gttacggcta ccgcgcagaa gatgcaacca accttgatga
    18121 aatctatgta aacagccgaa gccaaggttt tggtgaagag gtaaaacgtc gtatcatgct
    18181 gggtactttc agtctttcat caggttacta tgatgcctac tacaaaaagg ctggtcaagt
    18241 ccgtaccctc atcattcaag atttcgaaaa agtcttcgca gattacgatt tgattttggg
    18301 tccaactgct ccaagtgttg cctatgactt ggactcactc aaccatgacc cagttgccat
    18361 gtacttggct gacctcttga ccatacctgt aaacttagca ggtctccctg gaatttcgat
    18421 tcctgctgga ttctctcaag gtctacctgt cggtctgcaa ttgattggtc ccaagtactc
    18481 tgaggaaacc atttaccaag ctgctgctgc ttttgaagca acaacagact accacaaaca
    18541 acaacccgtg atttttggag gtgacaacta atgaactttg aaacagtcat cggacttgaa
    18601 gtccacgtag agctcaacac caattcaaaa atcttctcac ctacttctgc ccactttgga
    18661 aatgaccaaa atgccaacac taacgtgatt gactggtctt tcccaggagt tctaccagtt
    18721 ctcaataaag gggttgttga tgccggtatc aaggctgctc ttgccctcaa catggacatc
    18781 cacaaaaaga tgcactttga ccgcaagaac tacttctatc ctgataaccc caaagcctac
    18841 caaatttctc agtttgatga gccaatcggt tataaaggct ggattgaagt cgagctagaa
    18901 gacggtacga ccaagaaaat cggtatcgaa cgtgcccacc tagaggaaga cgctggtaaa
    18961 aacacccatg gtacagatgg ctactcttat gttgacctca accgccaagg ggttcccttg
    19021 attgagattg tatctgaggc agatatgcgt tctcctgaag aagcctatgc ttatctgaca
    19081 gccctcaagg aagttatcca gtacgctggc atttctgacg ttaagatgga ggaaggttcg
    19141 atgcgtgtgg atgccaacat ctcccttcgt ccttatggtc aagagaaatt cggtaccaag
    19201 actgaattga agaacctcaa ctccttctca aacgttcgca aggggcttga atacgaagtc
    19261 caacgtcaag ctgaaatact tcgctcaggt ggtcaaatcc gccaagaaac acgccgttac
    19321 gatgaagcta acaaagcaac catcctcatg cgtgtcaagg aaggggctgc tgactaccgc
    19381 tacttcccag aaccagacct gcctctcttt gaaatctcag acgagtggat tgaggaaatg
    19441 cggactgagt tgccagagtt tccaaaagaa cgtcgtgcgc gttatgtatc tgaccttggt
    19501 ttatcagact acgatgctag tcagttgact gctaataaag tcacttctga cttctttgaa
    19561 aaagctgttg ccctaggtgg tgatgccaaa caagtctcta actggctcca aggggaagtc
    19621 gctcagttct tgaatgctga aggtaaaaca ctggaacaaa tcgaattgac accagaaaac
    19681 ttggttgaaa tgattgccat catcgaagac ggtactattt catctaagat tgccaagaaa
    19741 gtctttgtcc atctagctaa aaatggcggt ggcgcgcgtg aatacgtgga aaaagcaggt
    19801 atggttcaaa tttcagatcc agctatcttg atcccaatca tccaccaagt ctttgccgat
    19861 aacgaagctg ctgttgccga cttcaagtca ggcaaacgta acgccgacaa agccttcaca
    19921 ggattcctta tgaaggcaac caaaggccaa gccaacccac aagttgccct taaactactt
    19981 gcacaggaat tggcgaagtt gaaagaaaac tagacagaac aaaaccagcc ctaaggttgg
    20041 ttttttcttg acaaatagaa agaaaacgtt tacaatattg cgtgtaaagt ttccaaacaa
    20101 gaaaactaaa aatcgcaaca ataaggagtt atttatatgg ctacaatgaa ggcagctcgc
    20161 tggcatgcag cgaaagatgt tcgtattcaa gaaatagaag ttcctgaagt acttccgcac
    20221 caagtaaaag ttgcagttaa gttcacagga atttgtggta ctgacctcca tgaattttta
    20281 gatggaccta tctttatccc aacagatgag cacgtctatt ctggtcaaaa agcaccggta
    20341 acactgggac atgaattttc tggtgaaatc atagaagtcg gaagcgatgt tacccgtgtt
    20401 aaagttggtg atcgtgttgc tgtggaacca attctagcta aaaataactt agttggtgat
    20461 tataatttgg atccaaacct taattttgtc ggtttggctg cagacggtgg atttgccaaa
    20521 tattgtgttt tagacggtga cttagtacat gtcattccag atagcttgag ctatgagcaa
    20581 gctgctctta ctgaacccgc tgctgttgca gtctatgctg ttcgtcaatc ttctttgaag
    20641 gctggcgata cagctgttgt ctttggttta ggcccgattg gtctattaat tgtagaagct
    20701 cttcgtgcag ctggtgcttc taagatttat gctgttgaac tatcacctga acgtcaagca
    20761 aaagctgaag agttaggagc tatcgttgtc cgtccggaag aaggtgaaac agctgttgaa
    20821 gctattcatc gtttaacaaa cggtggtgta gatgtttctt atgaagtaac gggtgtgcca
    20881 gtagttttag gacaagcctt agctgccgta cataaagcag gtgagtgcat ggtggtctct
    20941 atctgggaac gagaagcaaa tattaatccg aatgaatttg caatccaaga aaagacacta
    21001 aaaggaatca ttgcttatcg tcatatcttc cctaaagtat tagaattgat ggaacaaggc
    21061 tacttctctg ctgaaaaatt agttactaag aaaattaaat tggaaaatat cgtcgaagaa
    21121 ggctttatcg aattaactca agataagtca caaattaaaa ttttggtaga accagaataa
    21181 aaaatcaatc tttttaagac aatactaaca ggtcggagta aacttacttc ggccttttga
    21241 aatatagtac tcttcgaaaa tctcttcaaa ccacgtcagc gtcacattac cgtatatatg
    21301 gttactgact tcgtcagttc tatccacaac ctcaaaacag tgttttgagc agcctgcggc
    21361 tagcttccta gtttgctctt tgatttccat tgagtatgag aagaaatata gtggatactc
    21421 tttaacatta accactccac ctgactagcc aatattcttt ttcgctccta ccaactccca
    21481 ataactattt tggctttatt tccagagtat tttatggtaa aatgaagagt aataatattt
    21541 gttaaagagg taaaaacatg attgaagcaa gtaaattaaa agctggtatg acctttgaaa
    21601 cagctgacgg caaattgatt cgcgttttgg aagctagtca ccacaaacca ggtaaaggaa
    21661 acacgatcat gcgtatgaaa ttgcgtgatg tccgtactgg ttctacattt gacacaagct
    21721 accgtccaga ggaaaaattt gaacaagcta ttatcgagac tgtcccagct caatacttgt
    21781 acaaaatgga tgacacagca tacttcatga atacagaaac ttacgaccag tacgaaatcc
    21841 ctgtagtcaa tgttgaaaac gaattgcttt acatccttga aaactctgat gtgaaaatcc
    21901 aattctacgg aactgaagtg atcggtgtca ccgttcctac tactgttgag ttgacagttg
    21961 ctgaaactca accatctatc aaaggtgcta ctgttacagg ttctggtaaa ccagcaacga
    22021 tggaaactgg acttgtcgta aacgttccag acttcatcga agcaggacaa aaactcgtta
    22081 tcaacactgc agaaggaact tacgtttctc gtgcctaatc tctagaaaga ggtcattcta
    22141 tgggaattga agaacaactt ggcgaaatcg ttatcgcccc acgtgtactt gaaaaaatca
    22201 ttgctatcgc tactgcaaag gtagagggtg ttcactcttt ttcaaacaga tcagtgtctg
    22261 ataccctttc aaaactttca ctcggccgtg gcatttatct taaaaacgtg gacgaagaac
    22321 tcacagcaga tatctatctc taccttgagt acggagtaaa agttcctaag gtagcggttg
    22381 ctatccagaa agctgtcaaa gatgccgtcc gtaatatggc tgatgtagaa ctcgctgcta
    22441 tcaatattca cgttgcaggt atcgtcccag ataaaacacc aaaaccagaa ttgaaagatc
    22501 tatttgacga ggacttcctc aatgactagt ccactattag aatctagacg ccaactccgt
    22561 aaatgcgctt ttcaagctct catgagcctt gagttcggta cggatgtcga aactgcttgt
    22621 cgtttcgcct atactcatga tcgtgaatat acggatgtac aacttccagc ctttttgata
    22681 gacctcgttt ctggtgttca agctaaaaag gaagaactag ataagcaaat cactcagcat
    22741 ttaaaagcag gttggaccat tgaacgctta acgctcgtgg agagaaacct ccttcgcttg
    22801 ggagtctttg aaatcacttc atttgacact cctcagctgg ttgctgttaa tgaagctatc
    22861 gagcttgcaa aggacttctc cgatcaaaaa tctgcccgtt ttatcaatgg actgctcagc
    22921 cagtttgtaa cagaagaaca ataaggctct ttgtcaactg tagtgggttg aagaaaagct
    22981 aagctcgaga aaggacaaat ttcgtccttt cttttttgat gttcagagcg atgaaaatcc
    23041 gttttttgaa gttttcaaag ttccgaaaac caaaggcatt gcgcttgata agtttgatga
    23101 gattattggt cgcttccagt ttggcattag aatagtgtag ttgaagggcg ttgacaatct
    23161 tttctttatc tttgaggaag gttttaaaga cagtctgaaa aataggatga acctgcttaa
    23221 gattgtcctc aataagtccg aaaaatttct ctggttcctt attctggaag tgaaaaagca
    23281 agagctgata gagttgatag tggtgtttca agtcttccga ataactcaaa agcttgttta
    23341 aaatctcttt attggttaag tgcatacgaa aagtaggacg ataaaaacgt ttatcactca
    23401 gtttacggct atcctgttga atgagtttcc agtagcgctt gatagccttg tattcatggg
    23461 attttcgatg aaactgattc atgatttgga cacgcacacg actcatagca cggctaagat
    23521 gttgtacaat gtgaaagcga tcaagaacga ttttagcatt cgggagtgaa acagtctggg
    23581 agactgtttc agcctgagcc tagaaatttg aaagcgaagc tgtttagcca agtcatagta
    23641 agggctaaac atatccatag taatgatttt cacttgacaa cgaacggctc tatcgtagcg
    23701 aagaaagtga tttcggatga cagcttgtgt tctaccttca agaacagtga tgatatcgag
    23761 cttttcaaaa tcttgcgcaa taaagctcat ctccatctcc cgattgaaac agtcactccc
    23821 tggactgttt caacgtccca agacataatc ttaggcagat ttctaaaatt acactcaaag
    23881 tggaagtcat tgagctttcg aatgacagtt gaagttgaga tggaaagctg atgggcaata
    23941 tcagtcatag aaatcttttc aattaacttt tgagcaatct tttggttgat gatacgaggg
//
LOCUS       DBERMR010000028        23114 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.028, whole genome shotgun sequence.
ACCESSION   DBERMR010000028 DBERMR010000000
VERSION     DBERMR010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 23114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23114
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.028"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(157..381)
                     /locus_tag="ACL8A1_001276"
     CDS             complement(157..381)
                     /locus_tag="ACL8A1_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory protein Asp5"
                     /protein_id="HGS1202044.1"
                     /translation="MIEILIVLAIILSLALIVLVTIQPRQNQLFSMDATSNIGKPSYW
                     QSNTLVKVLTLLVSLALFVLLLTFMVITYK"
     gene            complement(386..565)
                     /gene="asp4"
                     /locus_tag="ACL8A1_001277"
     CDS             complement(386..565)
                     /gene="asp4"
                     /locus_tag="ACL8A1_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory system protein Asp4"
                     /protein_id="HGS1202045.1"
                     /translation="MSEEDLFYKDVEGRMEELKQKPIKKEKETRGEKISKTFSLLLGL
                     MILIGLLFTLLGILR"
     gene            complement(567..1904)
                     /gene="gtfB"
                     /locus_tag="ACL8A1_001278"
     CDS             complement(567..1904)
                     /gene="gtfB"
                     /locus_tag="ACL8A1_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031647 - regulation of protein stability
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HGS1202046.1"
                     /translation="MIELYDSYSQESRDLHESLGATGLSQLGVVIDADGFLPDGLLSP
                     FTYYLGYEDGKPLYFNQVPVSDFWEILGDNQSACIEDVTQERAVIHYADGMQARLVKQ
                     VDWKDLEGRVRQVDHYNRFGACFATTTYSADSEPIMTVYQDVNGQQVLLENHVTGDIL
                     LTLPGQSMRYFANKVEFITFFLQDLEIDTSQLIFNTLATPFLVSFHYPDKSGSDVLVW
                     QEPLYDAIPGNMQLILESDNVRTKKIIIPNKTTYERALELTDEKYHDQFVHLGYHYQF
                     KRDNFLRRDALILTNSDQIEQVEAIAGALPDVTFRIAAVTEMSSKLLDMLRYPNVVLY
                     QNASPQKIQELYQLSDIYLDINHSNELLQAVRQAFEHNLLILGFNQTVHNRLYITPDH
                     LFESSEVAALVETIKLALSDVDQMRQALGKQGQHANYVDLVRYQETMQTVLGG"
     gene            complement(1897..3408)
                     /gene="gtfA"
                     /locus_tag="ACL8A1_001279"
     CDS             complement(1897..3408)
                     /gene="gtfA"
                     /locus_tag="ACL8A1_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HGS1202047.1"
                     /translation="MTIYNINLGIGWASSGVEYAQAYRAGVFRKLNLSSKFIFTDMIL
                     ADNIQHLTANIGFDDNQVIWLYNHFTDIKIAPTSVTVDDVLAYFGGEESHREKNGKVL
                     RVFFFDQDKFVTCYLVDENKDLVQHAEYVFKGNLIRKDYFSYTRYCSEYFAPKDNVAV
                     LYQRTFYNEDGTPVYDILMNQGKEEVYHFKDKIFYGKQAFVRAFMKSLNLNKSDLVIL
                     DRETGIGQVVFEEAQTAHLAVVVHAEHYSENATNEDYILWNNYYDYQFTNADKVDFFI
                     VSTDRQNEVLQEQFAKYTQHQPKIVTIPVGSIDSLTDSSQGRKPFSLITASRLAKEKH
                     IDWLVKAVIEAHKELPELTFDIYGSGGEDSLLRDIIANHQAEDYIQLKGHAELSQIYS
                     QYEVYLTASTSEGFGLTLMEAIGSGLPLIGFDVPYGNQTFIEDGQNGYLIPSSSDHVE
                     DQIKQAYAAKICQLYQENRLEAMRAYSYQIAEGFLTKEILEKWKKTVEEVLHD"
     gene            complement(3420..5792)
                     /gene="secA2"
                     /locus_tag="ACL8A1_001280"
     CDS             complement(3420..5792)
                     /gene="secA2"
                     /locus_tag="ACL8A1_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HGS1202048.1"
                     /translation="MFRRLGQDFQLRKVKKILKQINALKGKMSSLSDQELVAKTVEFR
                     QRLSEGESLDDILVEAFAVVREADKRILGMFPYDVQVMGAIVMHYGNVAEMNTGEGKT
                     LTATMPVYLNAFSGEGVMVVTPNEYLSKRDAEEMGQVYRFLGLTIGVPFTEDPKKEMK
                     AEEKKLIYASDIIYTTNSNLGFDYLNDNLASNEEGKFLRPFNYVIIDEIDDILLDSAQ
                     TPLIIAGSPRVQSNYYAIINTLVTTLVEGEDYIFKEEKEEVWLTTKGAKSAENFLGID
                     NLYKEEHASFARHLVYAIRAHKLFTKDKDYIIRGNEMVLVDKGTGRLMEMTKLQGGLH
                     QAIEAKEHVKLSPETRAMASITYQSLFKMFNKISGMTGTGKVAEKEFIETYNMSVVRI
                     PTNRPRQRIDYPDNLYITLPEKVYASLEYIKQYHAKGNPLLVFVGSVEMSQLYSSLLF
                     REGIAHNVLNANNAAREAQIISESGQMGAVTVATSMAGRGTDIKLGKGVAELGGLIVI
                     GTERMESQRIDLQIRGRSGRQGDPGMSKFFVSLEDDVIKKFGPSWVHKKYKDYQVQDM
                     TQPEVLKGRKYRKLVEKAQHASDSAGRSARRQTLEYAESMNIQRDMIYKERNRLIDGS
                     RDLEDVVVDIIERYTEEVAADHYASRELLFHFIVTNISFHVKEVPDYIDVTDKTAVRS
                     FMKQVIDKELSEKKELLNQHDLYEQFLRLSLLKAIDDNWVEQVDYLQQLSMAIGGQSA
                     SQKNPIVEYYQEAYAGFEAMKEQIRADMVRNLLMGLVEVTPKGEIVTHFP"
     gene            complement(5785..6225)
                     /gene="asp3"
                     /locus_tag="ACL8A1_001281"
     CDS             complement(5785..6225)
                     /gene="asp3"
                     /locus_tag="ACL8A1_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HGS1202049.1"
                     /translation="MIITQRQSIYWGEVGGTYMYGTTVSYYLDKSVRLYNPLLPSGEI
                     LKTWFSSVNYQAARTQPQLPLLKRKQEYQLSLVFDCQPENGVYTKITFFDRYGDILEK
                     KVEKAKDFIFTYPEDSYTYQVSLLSAGFESLTFYHFSIKEIRSV"
     gene            complement(6222..7757)
                     /gene="asp2"
                     /locus_tag="ACL8A1_001282"
     CDS             complement(6222..7757)
                     /gene="asp2"
                     /locus_tag="ACL8A1_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HGS1202050.1"
                     /translation="MSKELNILQIGLANWENHYDIPENMSWYYFYPNSSKALREIIEK
                     EDINRFHAVLIEDGQYSRDLFSYVKYFEPYTLFYNQNLQINDREVVDFLKKRCAQAID
                     FLSPQQLINDLSKSLFGGGYGDKLFPPTIQVNPNFTGAISYQGLDYVSLEGEFGQDFA
                     QLAYWAYNIMVQKTLPIELWLEYEKEGNCDFRLVIRKMWSGSVDDFFEEVIVSEKDLE
                     QALFMDSRDGDYFLSISVEARGRGTIKLGNLHQRWSRKQFGKFVLGGNILHDSKRDEI
                     NYFFHPGDFKPPLTVYFAGYRPAEGFEGYFMMKTLGCPFILFSDPRLEGGAFYLGTDE
                     LEGKVKDTITHYLDYLGFDHKDLILSGLSMGTFPALYYGASFEPHAIIVGKPLANLGT
                     IASRGRLDAPGVSNLAFDCLIHHTGGTSSQDMTELDQRFWKIFKQANFSKTTFGLSYM
                     KDEEMDPQAYEQLVSYLCNTGAKILSKGTAGRHNDDTDTNISWFLHFYRMVLETGFGR
                     EKR"
     gene            complement(7741..9321)
                     /gene="asp1"
                     /locus_tag="ACL8A1_001283"
     CDS             complement(7741..9321)
                     /gene="asp1"
                     /locus_tag="ACL8A1_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HGS1202051.1"
                     /translation="MYYFIPAWYGSERTWHADITPWYFSHFRLEFDDTFHQIRLFQEQ
                     DIDSRLLVLAYQPHLRYFLYRHGVLETDTYSVFDVMQDFHNLHTQVLSIRDIEWDDDC
                     EFIYSPFTIIVQKNGKKFAKVEHGVEGFISDIQYFEPNGQIHMHHIMDDRGFVSSIIF
                     FEDGQAAYQEYLNPKGVWQFRERLKEGGQVEVNPILGYRFKMLTYQNMGDLVAEFFEN
                     YLQTYVKDQDIFMLPSHSHHDQLVLDRLPSTNPKLLSLFIGRNSQDTFRDLDVTFEKS
                     DLILVDREDSLRLLQELYPERMHQCYHLSSFDTRLRLGRSQTKKESIIYFQLDFEQGI
                     DNQALLQVLSFVAENKDTEVIFGAFAASQEQMNEVEGIVESFIQENIQSENLGKAIDY
                     GDAENPLEENQHQDLRLQCVNLNDELDLIKTLEFVRLIVDLNRHPHLYTQIAGISAGI
                     PQINLVETVYVEHLKNGYLLADVTEFSKAAHYYTDRLKEWNESLIYSIDKIKEHTGQQ
                     FLGKLEKWIEEVKNVKGT"
     gene            complement(9331..10548)
                     /gene="secY2"
                     /locus_tag="ACL8A1_001284"
     CDS             complement(9331..10548)
                     /gene="secY2"
                     /locus_tag="ACL8A1_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HGS1202052.1"
                     /translation="MTKIYSSITVKKGLFTLFLLFIYVLGSRITLPFVDLNSKDFLGG
                     STAYLAFSAALTGGNLRSLSIFSVGLSPWMSAMILWQMFSYSKKLGLSSTAIEIQDRR
                     RMYLTLLIAVIQSLAVSLSLPVQSSYSVILVVLMNTLLLIAGTIFLVWLSDLNASMGI
                     GGSIVILLSSIVLNIPQDVIETFKLVHIPTGIVLLLVFMTIIFSYLLALMYRARYLVP
                     VNKIGLHNRFKRYSYLEIMLNPAGGMPYMYVMSFLSVPAYLFTLLGFIFPNHSGLAAL
                     SKEFMVGKPLWVYVYISVLFLFSIIFAFVTMNGEEIADRMKKSGEYIYGIYPGADTSR
                     FINRLVLRFSVIGGLFNVVMAGGPMLFVLFDEKLLRLAMIPGLFMMFGGMIFTIRDEV
                     KALRLNDTYRPLI"
     gene            complement(10605..11282)
                     /locus_tag="ACL8A1_001285"
     CDS             complement(10605..11282)
                     /locus_tag="ACL8A1_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGS1202053.1"
                     /translation="MGISVAKGEYITIVDSDDIVKENMIETLYQQVQEKDADVVIGNY
                     YNYDESDGNFYFYVTGKDFCVEELAIQEIMNRQAGDWKFNSSAFILPTFKLIKKELFN
                     EVHFSNGRRFDDEATMHRFYLLASKIVFINDNLYLYRRRSGSIMRTEFDLSWARDIVE
                     VFSKKISDCVLAGLDVSVLRIRFVNLLKDYKQTLEYHQLTDTEEYKDICFRLKLFFDA
                     EQRNGKS"
     gene            complement(11287..11511)
                     /locus_tag="ACL8A1_001286"
     CDS             complement(11287..11511)
                     /locus_tag="ACL8A1_001286"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGS1202054.1"
                     /translation="MSELISVVVPIYNTGKYLVECVEHILKQTYQNIEIILVDDGSTD
                     NSGEICDAFMMQDNRVRVLHQENKGGGSTS"
     gene            complement(11511..12707)
                     /locus_tag="ACL8A1_001287"
     CDS             complement(11511..12707)
                     /locus_tag="ACL8A1_001287"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HGS1202055.1"
                     /translation="MRKSIVLAADNAYLIPLETTIKSVLYHNRDVDFYILNSDIAPEW
                     FKLLGRKMEVVNSTIRSVHIDKELFESYKTGPHINYASYFRFFATEVVESDRVLYLDS
                     DIIVTGELATLFEIDLKGYSIGAVDDVYAYEGRKSGFNTGMLLMDVAKWKEHSIVNSL
                     LELAAEQNQVVHLGDQSILNIYFEDNWLALDKTYNYMVGVDIYHLAQECERLDDNPPT
                     IVHYASHDKPWNTYSISRLRELWWVYRDLDWSEIAFQRSDLNYFERSNQSKKQVMLVT
                     WSADIKHLEYLVQRLPDWHFHLAAPCDCSEELTSLSQYTNVTVYQNVLHSRIDWLLDD
                     SIVYLDINTGGEVFNVVTRAQESGKKIFAFDITRKSMDDGLYDGIFSVGRPDDLVDRM
                     KNIEIE"
     gene            complement(12716..13936)
                     /locus_tag="ACL8A1_001288"
     CDS             complement(12716..13936)
                     /locus_tag="ACL8A1_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGS1202056.1"
                     /translation="MLQDKKSVVFAGDYAYIRQIETAMKSLCRHNSHLKIYLLNQDIP
                     QEWFSQIRIYLQEMGGDLIDCKLIGSQFQMNWSNKLPHINHMTFARYFIPDFVTEDKV
                     LYLDSDLIVTGDLTDLFELDLGENYLAAARSCFGAGVGFNAGVLLINNKKWGSETIRQ
                     KLIDLTEKEHENVEEGDQSILNMLFKDQYSSLEDQYNFQIGYDYGAAAFKHQFIFDIP
                     LEPLPLILHYISQDKPWNQFSVGRLREVWWEYSLMDWSVILNEWFSKSVKYPSKSQIF
                     KLQCVNLTNSWCVEKIDYLAEQLPEVHFHIVAYTNMANELLALTRFPNVTVYPNSLPM
                     LLEQIVIASDLYLDLNHDRKLEDAYEFVLKYKKPMIAFDNTCSENLSEISYEGIYPSS
                     IPKKMVAAIRSYMR"
     gene            complement(13961..14743)
                     /locus_tag="ACL8A1_001289"
     CDS             complement(13961..14743)
                     /locus_tag="ACL8A1_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGS1202057.1"
                     /translation="MKDLISVIVPVYNVEPFISSCLDSLSKQIYQNFEVLLVNDGSTD
                     NSGAICREYADRDSRFHYFEKENAGVADARNFGIERSKGDYITFVDSDGLFYIHVFDS
                     DYYVKNYNSKLLMEELPLLERYDMSFLTSWGILFKRELFQEVQFPFGRVCEDIGTNYK
                     LFMQVEKVTYINKVLYWYRVGKEGLSNSYSLKMMRDDCDFRLERIAVLALKGYDVSKY
                     LDQMKFYLKYRHDIAIQRELKENVETRRLEMLDYLLNGNKYN"
     gene            complement(14740..17193)
                     /locus_tag="ACL8A1_001290"
                     /pseudo
     CDS             complement(14740..17193)
                     /locus_tag="ACL8A1_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001044150.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
     gene            complement(17203..18212)
                     /locus_tag="ACL8A1_001291"
                     /pseudo
     CDS             complement(17203..18212)
                     /locus_tag="ACL8A1_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766254.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
     gene            complement(18241..19116)
                     /locus_tag="ACL8A1_001292"
     CDS             complement(18241..19116)
                     /locus_tag="ACL8A1_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGS1202058.1"
                     /translation="MENELISIVVPIYNVENYLRMCLDSIQNQTYQNFECLLINDGSP
                     DHSSKICEEFVEKDSRFKYFEKANGGLSSARNLGIECSGGAYITFVDSDDWLEHDALD
                     RLYGALKKENADISIGRYNSYDETRYVYMTYVTDPDDSLEVIEGKAIMDREGVEEVRN
                     GNWTVAVLKLFKRELLQDLPFPIGKIAEDTYWTWKVLLRASRIVYLNRCVYWYRVGLS
                     DTLSNTWSEKRMYDEIGAREEKIAILASSDYDLTNHILIYKNRLQRVIAKLEEQNMQF
                     TEIYRRMMEKLSLLP"
     gene            complement(19156..20034)
                     /locus_tag="ACL8A1_001293"
     CDS             complement(19156..20034)
                     /locus_tag="ACL8A1_001293"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HGS1202059.1"
                     /translation="MKKNHLVGDALILTVSDQIEELDYLLESLPNICFHIAAPVQFSE
                     KIRSLETNYNVRLRTITNEEQLNFLVDTCDFLLDINHFQEVDAIVSKFVQAGKSVFAF
                     DNTVHGNQGQEVFLSSTPDKLVSRVRDYLNEVRVGTNHQEKIIQDGTWNVFKIDDKAH
                     FIVGANVICRNFENFHVSSGKLILHDGVFINNSCSFNCMERIEIGAGTMMGEGVRFYD
                     HDHIYTAEKIEKWQWTTAPIRVGRDCWIGSNVTILKGVTIGDNTIIGAGCLIRNDIPS
                     NSVVYNNGNLVVKRRD"
     gene            complement(20031..22115)
                     /locus_tag="ACL8A1_001294"
                     /pseudo
     CDS             complement(20031..22115)
                     /locus_tag="ACL8A1_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000341103.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(22176..22904)
                     /locus_tag="ACL8A1_001295"
     CDS             complement(22176..22904)
                     /locus_tag="ACL8A1_001295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HGS1202060.1"
                     /translation="MSTNASASASTSASESASTSASASASTSASASASTSASASASTS
                     ASESASTSASASASTSASASASTSASASASTSASASASTSASESASTSASDSASTSAS
                     ASASTSASASASTSASASASTSASESASASTSASASTSASASASTSASASASTSASAS
                     ASTSASASASTSASASASTSVSNSANHSNSQVGNTSGSTGKSQKELPNTGTESSTGSV
                     LLGVLAAVTGIGLVAKRRKRDEEE"
     gene            22873..>23114
                     /locus_tag="ACL8A1_001296"
     CDS             22873..>23114
                     /locus_tag="ACL8A1_001296"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HGS1202061.1"
                     /translation="MLAEAEAFVDTEAEALVDAEADALVDAEAEALVDADSDALVDAE
                     ADALVDAEAEALVDAEAEVLVNAEAEALVDAEAEALV"
BASE COUNT         7332 a         4957 c         3642 g         7183 t
ORIGIN      
        1 gtgttttgag ctgacttcgt cagttctatc tacaacctca aaacggtgtt ttgagcagcc
       61 tgcggctagc ttcctagttt gctctttgat tttcattgag tataaaaaag aaaacttcag
      121 accaatccac aaaaggtgaa tatctgaagt tttcttttat ttataagtaa tcaccataaa
      181 ggttaatagt agaacaaata aagccaaact caccaataaa gtgagcacct tgaccaaggt
      241 gttgctctgc cagtagcttg gtttaccaat attactagtg gcatccatgg aaaatagttg
      301 attttgacgg ggttgtatag ttaccaatac aatcaaagca agagatagga taatagctaa
      361 aacaattagt atttcaatca tagatctacc tcaaaattcc cagcaaagta aagagcaaac
      421 caatcagaat cattaagccc agtaaaagtg aaaaagtctt actaatcttt tcccctcggg
      481 tttctttttc cttcttgatg ggtttttgtt tcaactcttc catgcggcct tcaacgtctt
      541 tgtaaaataa atcttcctct gacatgttag cctcctaaaa cagtttgcat ggtttcctga
      601 tatctcacca agtcaacata atttgcatgt tggccttgtt tgccaagtgc ctgacgcatt
      661 tgatcaacat ctgaaagggc caatttaatg gtctcaacca aagcagcaac ttcactactt
      721 tcaaatagat ggtctggagt gatataaagt ctattgtgca ccgtctgatt aaagccaaga
      781 atcaagagat tgtgctcaaa ggcctgacgc actgcctgta gcaactcatt actgtggttt
      841 atatccaagt aaatatccga cagttgatac agctcctgaa tcttctgtgg actagcgttc
      901 tgataaagga ccacattagg ataacgaagc atatctaaga gcttagaaga catctctgtc
      961 accgctgcaa tacggaaagt gacatcaggc aaggctcctg cgattgcttc tacttgctca
     1021 atctgatctg aattggtcaa gattaaggca tctcgtctta ggaaattatc acgtttgaac
     1081 tggtaatgat aacccaagtg cacaaactga tcatggtatt tctcgtcagt taactctaaa
     1141 gcgcgctcat aagtcgtctt atttggaatg atgatcttct tagtacgcac attatcactt
     1201 tccaaaatca actgcatatt acctggaatg gcatcataaa gaggttcctg ccataccaag
     1261 acatccgagc cagatttatc tggataatga aaggaaacca agaaaggagt cgctagagta
     1321 ttaaagataa gctgactggt gtctatttcc aaatcttgca aaaagaaggt gataaattca
     1381 actttatttg caaagtaacg catggactga ccaggcaaag tcaataagat atcacccgtc
     1441 acatggtttt ccagtaaaac ttgttgacca ttgacatctt ggtaaactgt cataatcggc
     1501 tcgctatctg cgctataagt cgttgtagca aaacaagctc cgaagcgatt gtagtggtca
     1561 acctgacgta ctcgaccttc taggtctttc cagtctacct gtttaaccaa gcgagcctgc
     1621 attccatcag cataatgaat gacagccctc tcctgcgtca catcttcaat acaagcagac
     1681 tgattatctc ctaaaatttc ccaaaaatct gaaacgggaa cttgattaaa atagagaggt
     1741 tttccatcct cgtaacctag ataataggta aaaggagaaa gcagaccatc aggcagaaaa
     1801 ccatctgcat cgatgaccac tccaagttga gaaagaccag tagcgcctag actttcatgt
     1861 aaatctcgac tttcttgact gtaactatca taaagttcaa tcatggagca cctcctctac
     1921 tgttttcttc cacttttcta aaatttcttt ggtcaagaag ccttctgcaa tttggtaaga
     1981 ataggcacgc atagcttcca aacgattttc ttgatacaat tgacaaatct tagcggcata
     2041 agcttgcttg atttggtctt ctacatggtc agatgaactt ggaatcaaat aaccattttg
     2101 cccatcctct ataaaggtct gattaccata aggcacatca aaaccaatta gaggtagacc
     2161 tgaaccaata gcttccatca aggtcagacc aaatccttcg ctggtagaag ccgttaagta
     2221 gacctcatac tggctataaa tctgcgaaag ttccgcatgc cccttgagtt ggatatagtc
     2281 ctctgcctga tgatttgcaa taatgtctct aagcagagaa tcttctccac cactaccata
     2341 gatatcaaag gttagttccg gtaactcctt atgagcttca atcacagctt tcacaagcca
     2401 atcaatgtgc ttttctttgg caagacgtga agctgtaatc aatgaaaatg gtttgcgccc
     2461 ttgacttgaa tctgtcaagg aatcaatact gcctacagga atggtaacaa tctttggctg
     2521 atgctgagta tatttggcaa attgctcttg tagaacttca ttttgtctat cagtagacac
     2581 gataaagaag tcaaccttat ctgcattggt aaactgatag tcataatagt tattccaaag
     2641 gatgtagtcc tcatttgtag cattttcact ataatgctcc gcatgaacaa ctaccgctaa
     2701 atgtgctgtc tgtgcttcct caaacacaac ctgaccaatg cctgtctccc tatcgagaat
     2761 gaccaaatca gacttattca aattcaaaga tttcataaag gcacgcacaa aagcttgctt
     2821 tccatagaaa atcttatcct tgaaatgata aacttcttcc ttcccttgat tcatcaagat
     2881 atcatagact ggagtcccgt cttcattata aaaagttcgt tggtataaga ctgcaacatt
     2941 gtccttggga gcaaaatact cgctacaata acgcgtataa gaaaagtaat ccttccgaat
     3001 caggtttccc ttaaaaacat actcggcatg ttgaaccaag tccttgttct catcaaccaa
     3061 ataacaggtt acaaacttat cttggtcaaa aaagaagaca cgtaaaacct tgccattttt
     3121 ttctctgtga ctttcttcac caccaaagta agccaagaca tcatccactg tcacgctagt
     3181 aggcgcaatt ttgatatctg tgaaatgatt ataaagccag ataacctgat tatcatcaaa
     3241 accaatattg gctgttaagt gctgaatatt atcggctaaa atcatatctg taaagataaa
     3301 cttagaggac agatttaatt tccgaaaaac accagcacga taggcttgag cgtattcaac
     3361 accgctacta gcccaaccaa ttcctaaatt tatattgtaa attgtcatat tttctcctct
     3421 tatggaaaat gagtcacgat ttcacctttt ggagtgacct caaccagccc catcaggaga
     3481 ttacgcacca tatccgcacg gatttgttct ttcatagctt caaagcccgc gtaggcttct
     3541 tgatagtact ctacgattgg atttttctga ctagcagatt gaccaccgat agccatggat
     3601 agctgttgta gatagtctac ctgctctacc cagttgtcat caatggcttt aagcagtgaa
     3661 agtcgtaaaa actgttcata taagtcatgt tgattaagta attctttctt ttcagaaagt
     3721 tctttatcaa tcacctgctt cataaagcta cgaactgcag ttttgtcagt tacatctata
     3781 taatctggaa cctctttaac atgaaaacta atattggtca caataaagtg aaacagtaat
     3841 tcacgactag catagtgatc agccgctacc tcttctgtat atctctcaat gatatccaca
     3901 acaacatcct ctaagtcacg agaaccatct attagacgat tacgctcctt gtaaatcata
     3961 tcccgttgaa tattcatact ttcagcatat tctagagtct gacgacgtgc cgaacggcca
     4021 gcactatcac tggcatgctg agccttttcg actagtttcc ggtatttacg acctttcaat
     4081 acttccggtt gagtcatatc ttgaacctga tagtctttgt actttttatg cacccaagat
     4141 ggaccaaatt tcttgataac atcatcctct aaggatacaa aaaatttact cataccagga
     4201 tctccctgac gaccagaacg gccacgaatt tgtaggtcga tccgctgact ttccatccgc
     4261 tcagtcccaa taacaatcaa gcccccaagc tctgcgactc ctttaccaag cttgatatcc
     4321 gtaccacgtc ctgccataga ggtagccact gtcacagccc ccatctgacc tgactcggag
     4381 ataatctgag cctcacgcgc cgcattatta gcatttagga cattatgggc aatcccttca
     4441 cgaaacaaga gagacgaata gagttgagac atttcaactg agcctacaaa aacgagtaag
     4501 ggatttccct tagcatggta ttgcttgatg tactccaagg atgcatacac tttttcaggt
     4561 aaagtgatat atagattatc tggatagtca atccgttgtc tcggacgatt ggttggaatg
     4621 cgtactacag acatattgta agtttcaata aactcttttt ccgcgacctt acctgtccct
     4681 gtcataccag atatcttatt aaacatctta aaaagactct gataggtgat cgaggccata
     4741 gcccgcgtct caggagataa tttgacatgt tccttggctt caatagcctg atggagacct
     4801 ccttgaagtt tagtcatttc cattagacgc cctgtgccct tatcaaccag taccatctca
     4861 tttccacgaa tgatatagtc cttatcttta gtaaagagct tatgagctcg aatcgcataa
     4921 accaaatgac gagcaaaaga cgcatgctct tccttgtata aattatcaat ccctaggaaa
     4981 ttctcagcag acttggcccc cttagtagtg agccaaacct cctctttctc ctctttaaag
     5041 atataatcct ctccttcgac caaggttgtt acaagtgtat taatgatcgc atagtaatta
     5101 gactgaacac gaggagaacc cgcaataatc agaggagttt gtgcactatc aagcaagata
     5161 tcatcaattt catcaataat cacatagtta aacggtcgta aaaacttacc ttcttcattc
     5221 gaggctaggt tatcatttag ataatcaaaa cctaaattac tattggttgt gtagatgata
     5281 tccgaagcat agataagctt cttttcttca gctttcatct ccttctttgg atcttccgta
     5341 aatggtacac caatggtcaa tcctagaaaa cgataaactt gacccatttc ctcggcatca
     5401 cgctttgata aatactcatt aggagtcaca accatcactc cttctcctga aaaagcgttc
     5461 aaatagacag gcatggtagc tgtcaaggtc ttaccttccc ccgtattcat ctcagcaaca
     5521 tttccatagt gcatgacaat agctcccatg acttgaacat cataaggaaa catccctaaa
     5581 atccgcttat ctgcttcacg caccacagca aaagcttcaa ccaaaatatc gtctagactt
     5641 tctccctcgg aaagacgctg acgaaactct actgttttag ctactaattc ttgatccgaa
     5701 agagaggaca tcttgccttt cagggcatta atctgcttta aaatcttttt cactttccta
     5761 agctggaaat cttgacctaa acgtctaaac acttctgatc tccttgatag aaaaatgata
     5821 aaaagttaag gactcaaatc cagcacttaa aagagaaact tgataagtat aactatcttc
     5881 tggataagta aatatgaaat ctttcgcttt ttctaccttt ttttctaaaa tatcaccata
     5941 gcggtcaaaa aaagttatct tggtataaac tccattttca ggctgacaat caaaaaccag
     6001 tgatagttga tactcctgct ttctttttaa taagggaagc tgaggttggg ttcgtgcagc
     6061 ctggtaattg acactagaaa accaagtctt tagaatctca ccagatggca atagaggatt
     6121 atacagacga acacttttgt caaggtaata agatacagtt gttccataca tataagtccc
     6181 accaacttct ccccaataaa tagactgtct ctgtgtaata atcatctttt ctcccttcca
     6241 aaaccagtct ctaagaccat tctataaaag tgcaaaaacc aagaaatatt ggtatctgta
     6301 tcatcattgt gtcgtccagc agttccttta gataaaatct tcgcacctgt attacacaga
     6361 taagacacta attgttcata ggcttgtgga tccatttctt catctttcat ataggataaa
     6421 ccaaaggttg tctttgaaaa atttgcttgt ttaaaaattt tccaaaaacg ctgatccaac
     6481 tccgtcatat cttgagaact tgtcccacct gtatgatgaa ttaaacaatc aaaagctaag
     6541 ttagagacac ccggtgcgtc caaacgtcca cgactagcta tagttcctaa attagccaag
     6601 ggcttaccaa ctatgatggc atggggttca aaagaagcac cataatagag agccggaaac
     6661 gttcccatag aaagacctga taaaatcaaa tccttatgat caaagcctaa ataatcaaga
     6721 tagtgagtga tcgtatcctt tacttttccc tctagctcat ccgttccgag ataaaaagct
     6781 cccccctcta aacgtggatc agaaaataaa atgaagggac atccaagagt tttcatcata
     6841 aagtaaccct cgaatccttc tgcaggtcga taacctgcaa aatagacagt caaaggcggt
     6901 ttaaaatcac ctggatggaa gaaatagttt atttcatcac gcttggaatc atgtaggata
     6961 tttccaccaa gtacaaactt accaaattgt tttcgactcc atcgttggtg aagattacct
     7021 agtttgatag ttccacgacc tcttgcttca acagatatcg agagaaagta gtctccgtct
     7081 cgactatcca taaaaagtgc ttgctccaag tctttttcag atactattac ttcttcaaag
     7141 aaatcatcaa cagacccact ccacatttta cggatgacca aacgaaagtc acaattgcct
     7201 tccttctcat attcaagcca caactcaata gggagtgttt tttgcaccat aatattataa
     7261 gcccaatagg caagctgggc aaaatcttgc ccaaactcac cttccaaact gacataatcc
     7321 aatccttgat aagaaatagc tcctgtaaaa tttggattga cttgtatcgt cggaggaaag
     7381 agtttatcac catacccacc gccaaaaaga gatttactta aatcattgat tagttgttgg
     7441 ggacttaaaa aatcaattgc ttgtgcacat cgttttttta gaaaatccac aacctctcta
     7501 tcatttattt gtagattctg gttatagaac aaagtataag gttcaaaata ttttacatag
     7561 gaaaataagt ctctggaata ctgaccatct tctattaaaa ctgcatgaaa acggttaata
     7621 tcctcttttt caattatttc acgaagggct tttgatgagt ttgggtaaaa ataataccaa
     7681 ctcatatttt caggtatgtc atagtgattt tcccaattgg caagtcctat ctgtaaaata
     7741 ttaagttcct ttgacatttt taacctcctc tatccatttc tctaattttc caagaaattg
     7801 ttgtcctgtg tgctccttaa tcttatcaat tgaatatatc aaggactcat tccactcctt
     7861 caacctatct gtgtaataat gtgcagcctt agaaaattct gtaacatctg ctaacaaata
     7921 accatttttt aaatgttcaa catagacggt ttcaactagg ttgatttgag gaattcctgc
     7981 actaatccca gcaatctgtg tgtagagatg aggatgtcta tttaaatcca caatcaaacg
     8041 gacaaattct agtgttttta tcaaatctaa ctcatcattc aagttaacac actgtaagcg
     8101 taagtcctgg tgttgatttt cttccagagg attttctgca tcaccataat caatcgcctt
     8161 tcccagattt tcggattgaa tgttttcttg gatgaaagac tcaacaatcc cttcaacctc
     8221 attcatttgc tcctgactag cagcaaaggc tccaaaaatc acctcagtat ccttattttc
     8281 agcgacaaag gacaagactt gaagcagagc ttgattatca atcccctgct caaaatccag
     8341 ttgaaaataa atgatggatt ctttcttagt ttggcttcgt cccaatcgta atcgggtgtc
     8401 aaaagatgat aaatgataac attgatgcat tcgttcagga tacaactcct gcaacaatcg
     8461 taaactatcc tctctatcca ccaaaatcaa atccgatttt tcaaaagtta catctaaatc
     8521 cctaaaggta tcttgagaat tacgtccaat gaacagactc aacagtttag gattagtact
     8581 aggtaaacga tctagtacca actggtcatg atgagaatga gaaggaagca taaaaatatc
     8641 ctgatccttc acatacgttt gcagataatt ctcaaaaaat tctgccacca gatctcccat
     8701 attttgatag gtaagcattt taaagcgata acccaaaatt ggattgactt ctacctgtcc
     8761 tccttctttt aaacgctctc taaattgcca aactccctta ggattcaaat attcttgata
     8821 ggctgcttgc ccatcttcaa aaaagataat gctcgataca aaccctcgat catccatgat
     8881 atggtgcata tgtatttgac cattcggttc aaaatactgt atatcactga tgaagccttc
     8941 aactccatgt tcaaccttag caaatttctt cccatttttt tgaacgataa tcgtaaaggg
     9001 actataaata aattcacagt catcatccca ctcaatatct ctaatgctta aaacttgggt
     9061 atggagatta tgaaaatctt gcataacatc aaaaacggaa taagtatccg tttctaacac
     9121 accatgtcta tataaaaaat aacgtagatg aggctggtaa gctaatacta atagacgaga
     9181 atctatatct tgctcttgaa agagccggat ctggtgaaag gtatcatcaa actctagacg
     9241 aaaatgagaa aaataccatg gagtgatatc tgcatgccat gttctttctg acccatacca
     9301 agctggaata aaataataca taaaagtctc ctaaatcaag ggtctatagg tatcatttag
     9361 ccttaaagcc ttgacctcgt ctctaatcgt aaaaatcatg cccccgaaca tcataaataa
     9421 gcctggaatc attgccaatc gtaataactt ttcatcaaac aaaacaaaaa gcatgggacc
     9481 acctgccata accacattaa agagaccacc tatgactgag aaacgaagga ccaatcgatt
     9541 aataaatcga ctagtatccg cacctggata aataccataa atgtattctc cagatttttt
     9601 catacggtct gcaatctctt ctccattcat cgtaacaaaa gcaaaaatga tactaaataa
     9661 aaataagacc gaaatataaa catagaccca caaaggctta ccaaccataa attcctttga
     9721 taaagccgct agccctgaat gattagggaa aataaatccc aacaaggtga acaaataagc
     9781 tggtacacta agaaaactca tcacatacat ataaggcatc ccacctgcag gattcaacat
     9841 gatttcgaga taagaatagc gtttaaatcg attgtgtaag ccaattttat taacaggaac
     9901 caaatagcga gctcgataca taagggcaag caagtaagaa aatataatgg tcataaaaac
     9961 aagcaagaga acaatccctg tagggatatg aaccagttta aaagtttcaa taacatcctg
    10021 agggatattt aaaacaatgc tggataggag gattacaata gaacccccaa ttcccatgct
    10081 agcatttaaa tctgacaacc aaaccagaaa aattgttcct gctatcagca acaaggtatt
    10141 cattagaaca accaaaatta ccgagtagga ggattgtact ggcaagctca agctaactgc
    10201 caaggattga atcacagcaa ttagcaaggt cagatacatt cttcgacgat cttgaatttc
    10261 aatcgctgta gaacttagcc ccaatttttt agaatatgaa aacatctgcc acaaaatcat
    10321 agctgacatc caaggggata atccaacaga aaaaattgac aaacttctta gatttccacc
    10381 tgtaagggct gctgaaaaag ctaggtaggc tgttgaacca cctaagaaat ctttggaatt
    10441 caaatcaaca aaagggagag taatacgact tcccaataca tagataaaca gtagaaataa
    10501 ggtaaataat ccttttttta ctgttataga cgaataaatc ttagtcataa aacacgcccc
    10561 tcaccttcat tgtttgtgat atggtaaata acaattcttt tatttcaact tttaccattt
    10621 ctttgttctg catcaaaaaa caactttaat ctgaaacaaa tatctttata ttcctcagta
    10681 tctgttaatt gatggtattc taaagtttgc ttataatctt ttaaaagatt gacaaatcga
    10741 atacgcagaa cggagacatc caaaccagcc aagacacaat ccgatatttt cttagaaaac
    10801 acttcaacaa tatctcttgc ccaggaaaga tcaaattccg ttctcatgat gcttcctgaa
    10861 cgtcttctat acagatagag attatcgttt ataaagacga ttttagaggc taaaagataa
    10921 aagcgatgca tagttgcttc atcatcaaag cggcgaccat ttgaaaagtg aacttcattg
    10981 aataattctt ttttaatcaa cttaaatgtc ggcaatataa aggccgagct attgaatttc
    11041 caatctcctg cttgacggtt cataatttct tgtatagcta attcttcgac gcaaaaatct
    11101 ttccctgtta cataaaaata aaaattcccg tcactttcgt cataattata gtaattccct
    11161 ataacaacat ctgcatcctt ttcttggact tgctgataaa gagtttcaat catattttct
    11221 tttacgatat catctgaatc aacaatcgtg atgtactctc ccttagctac actaatcccc
    11281 atatttttag cttgtgctgc cccccccctt attttcttga tgcaatactc gcacacgatt
    11341 atcttgcatc ataaaagcat cacaaatttc cccagaatta tccgtagaac cgtcatcaac
    11401 taaaataatt tctatatttt gataggtttg cttcagaata tgctcgacac actccactaa
    11461 atattttccc gtattgtata tcggtaccac aacactaatt aattcactca ttactctatc
    11521 tctatattct tcattctatc cactaaatca tctggtctcc ccacagaaaa aataccgtca
    11581 tagagtccat catccatact tttacgtgtg atatcaaaag cgaagatttt cttgccactt
    11641 tcttgtgccc ttgtaactac attaaaaacc tctccacctg tattaatatc taaataaact
    11701 atagaatcgt ccaatagcca atcaattcta ctatgtaata cattttgata tactgttaca
    11761 ttcgtatact gtgatagaga ggtcagctcc tcagaacaat cacacggtgc agccaaatga
    11821 aaatgccaat caggtaaccg ttgtactaaa tactctaaat gttttatatc tgcactccat
    11881 gtcacaagca tcacttgttt tttagactgg ttgcttcttt caaaataatt taaatcggaa
    11941 cgttgaaaag caatctctga ccaatccaaa tctctataaa cccaccataa ttcacgtagt
    12001 ctagatatac tatatgtatt ccaaggttta tcatgactag catagtgaac aattgtaggt
    12061 ggattgtcat ctagacgttc acattcttga gcaaggtgat aaatatcaac acccaccata
    12121 taattatatg ttttatctaa ggctagccaa ttatcctcaa aataaatatt taaaatactc
    12181 tgatccccaa gatgaacaac ttgattctgc tcggccgcta attccaataa actattgaca
    12241 atagaatgtt ctttccactt tgcaacatcc attagtaaca taccagtatt aaatccagat
    12301 tttcgtcctt cataggcata gacatcatca acagcaccaa ttgaatatcc tttgagatct
    12361 atctcaaaca aagtagctag ttccccagtt acaatgatat cggaatccag atacaatacc
    12421 ctatcagatt caaccacttc tgtcgcaaaa aatctaaagt aagaagcata atttatatga
    12481 ggtcctgttt tatagctttc aaaaagttct ttatcaatgt gtacactgcg aattgtagaa
    12541 ttcacaactt ccatttttct ccccaataat ttaaaccatt caggagctat atcactgttg
    12601 agaatataaa aatcaacatc tctattgtga tacaatacag actttatagt cgtctctaaa
    12661 ggaataagat aggcattatc tgccgctaat actattgatt ttctcatact gttctctacc
    12721 tcatgtaaga tctgattgct gcaaccattt ttttcggaat ggagcttgga tagatacctt
    12781 catatgaaat ctcagaaaga ttttcagagc aagtattgtc gaaagctatc attggttttt
    12841 tgtacttaag cacaaactca tatgcatctt ctaattttcg atcatgattc aaatccaaat
    12901 acaaatctga agctattact atttgttcca ataacattgg taaggaattt ggatatacgg
    12961 taacattagg aaaacgcgtt aaagctagta gttcatttgc catatttgta taagcaacaa
    13021 tatgaaaatg aacttctggc aattgctccg ccaaataatc gattttctcg acacaccaag
    13081 aattcgttaa attaacacat tgtaacttaa atatttgtga tttactagga tacttcacac
    13141 tctttgaaaa ccattcattt aaaataacag accaatccat caaagagtat tcccaccaaa
    13201 cttctcttag acgtccaaca gaaaattgat tccaaggctt atcctgagaa atatagtgta
    13261 aaattagtgg cagtggttcg agcggaatat caaaaatgaa ttgatgttta aaggctgccg
    13321 ccccataatc atatcctatt tgaaaattat attgatcttc aagggaacta tattgatctt
    13381 taaacaacat attcaaaatt gactggtctc cttcttccac attctcatgt tctttttctg
    13441 ttaagtcaat caatttttgt cgaatagttt cagatcccca ttttttgttg ttaatcaaga
    13501 gaacaccagc attgaagccg actcctgctc caaagcaaga acgagctgct gccaaataat
    13561 tttcacctaa gtctaattca aacaaatcgg tcaaatcacc agtcacaatc aaatcactat
    13621 ctagatagag aactttatct tctgttacaa aatctggaat aaaatagcgt gcaaatgtca
    13681 tatgattgat atgaggtaat ttattagacc aattcatttg aaactgtgag ccaattaact
    13741 tgcagtcaat caagtcgccc cccatctctt gtaaatatat tcttatttga ctaaaccatt
    13801 cctgaggaat gtcctgattt agcagataaa ttttcaaatg actattgtgt ctacagagtg
    13861 acttcatcgc cgtttcgatt tgtcgaatat aagcgtaatc acctgcaaat actacagatt
    13921 tcttatcttg taacatttta tcgtctctct atcttaattt tcagttatat ttattaccat
    13981 tcaataaata gtctaacatt tctaaacgtc ttgtttccac attctcctta agttctcgtt
    14041 gaattgcaat atcatgacga tatttcaaat agaacttcat ctggtctaaa tatttactga
    14101 catcatatcc ttttagcgct agcacggcaa ttcgttctaa cctaaaatca caatcatcac
    14161 gcatcatctt cagtgaataa gaattagaaa gtccttcttt cccaacccta taccaataca
    14221 aaactttatt aatataggtt actttctcaa cctgcataaa taatttataa ttggttccta
    14281 tatcctcaca cacccgacca aacggaaact gaacttcttg aaataattcc cttttaaata
    14341 gaattcccca agaagttaaa aagctcatat catacctctc taataaagga agttcttcca
    14401 ttagaagttt tgaattataa tttttcacat aataatcgga gtcaaatacg tgaatataga
    14461 ataagccatc tgagtctaca aaagtaatat aatctccttt agatctctcg attccaaaat
    14521 tacgtgcatc cgctacccca gcattttctt tttcgaaata gtgaaaacga ctatcacgat
    14581 cagcatactc tcgacatatt gctcctgaat tatctgtaga gccatcattt actaataaaa
    14641 cttcaaaatt ttgataaatc tgtttcgata aactgtctaa gcagctagaa ataaatggtt
    14701 ctacgttata aactggtact ataacactta ttaaatcttt catacttctc tttttgataa
    14761 atcaactaaa atctgactgt tataggtttc atcatcaata aattcaatat cttggtcgaa
    14821 attatgttct gcagtatgtt tatgagaaaa tttaacctta gttttagctc ccattttcat
    14881 ccattcatac tctgagtcaa tatggcctac atccaaaact tgatagccca tctggcatag
    14941 attataactc agaacttttg ctgtaggacc cagcatacat aaaatcagtc ttccttcagc
    15001 atgtttcata atttcctctt gaatgttatc gacaacggaa taagcattat gagatggaca
    15061 aataatccgt ttcacagatt tgaccttatc aaataaatca tttccaaccc cagagcgaga
    15121 tgtaatcccc tctacaatca gaatatccct attctcccaa atactcttta gcttttcaaa
    15181 ttgtcctttt gccttactct tatcttcata atcaatgtat ggacgcgaga taaaggtaga
    15241 gccataccat gaacccttac acaactcttt ataaaaatcc atataaacat tcacatgttc
    15301 tttccagaat ggtgtagctc accaagtaaa gacaaaacga tcgtcaaaag catcaggtaa
    15361 acaaaccact aatttctcgt cactctcaag acccacaata tgttttaatt ggttagctaa
    15421 ctctagatca tgattttgat aaggtatagg caaaccccac atcaaattga cttctccgtc
    15481 tccaaaacga actaaagaag agttgtgttc aataatataa tccaaggttt catctatccc
    15541 tttcacctgg attatatcaa gcggtttttc tagcatatcc ttagttatca atttttgcaa
    15601 atcagccaac attcgttcag gatgttcact tgaatacaac ctttggccat tattcccatt
    15661 ttgagattta taaaaggcta gtataggttt agatagatga gccatttctg caacgatatc
    15721 ggatgttgaa gtcagtaggt tgatatctaa ataagcatcc atcttctcct tcaactcgtc
    15781 tagcttatct ctagaaattg ccggatagag ctgaatatta ggataacgat tagacaaatc
    15841 atataatttt ggccccatag ctgtccaagc agcaacttga aaaacaacat ttggcaactg
    15901 tccagccaag taatccaatt gttcgatatc ctgagaagcc gtaaacgtta aacaagttaa
    15961 acttggaagt tgtcgaaaac aggtaagatg tccgtttttc ttaaaatctt taatcgtatc
    16021 aatcataaag gaaactttcc cattttcgaa aacgatttgt cctaactctc cgtgctgagt
    16081 cgattgaaaa gctagtagcg ttttttcttg ttcttgcaaa gattttaaaa agttctcatc
    16141 tgcactacca tgattgatat ccaaatatag atttgttgac tttttcagct tatctaatac
    16201 aggaggaaca atctgtggat gcaatctcac attattatat acagctaatt tttttaattt
    16261 atctcccata tccgtccaag ctgcgatatg aaataccacc tcaggtagag ctgtaactag
    16321 ctcttctatt ccttctaaat tttgggaatt cgttaaggtt aagcaagaaa attccttatc
    16381 tagaggcgat attagttcaa attctcccat atggtgttgt aaaatctgac tccactccaa
    16441 atcatggaat tcccaccata aatcacgata acgattagct gtcaaggtgg tccagggttt
    16501 tctgtaggtc gtaaaatgaa tcaccactgg tttatcgtta aaagccagat gttcctgcca
    16561 attgttatac aaagccacaa tatcatgccc tacttgtaaa ttataagctc gacctagctc
    16621 taaccaatca tcttgtaaga cctgattaaa aatcgtttga tcaccattaa aatgctcgaa
    16681 acggccttct tcaacttcct tcattgtaac aatgctctgt tcaattagtc gttcttttaa
    16741 tttctcttga cgccattttt tattgttgat taatagaaca cctgtattaa atgtaattcc
    16801 atctgtatct ttaactgctg cgagtgattt ttcttctaaa caaatactaa ataatttctc
    16861 caaagaagta tttacaatca aatcactgtc taaatataaa accttatctt cttggatata
    16921 atctgcaata aaatatctag cataggtaat gctactaatg tgatcttgct tttcccaatc
    16981 ttgaaacaca gtttgttcag gtagtttaac atcgataatc tcactaccta acatgcgagc
    17041 tatttttcgt ggtttgcgaa accaatctgg catgatatct tgattcaaaa tataaatctt
    17101 aacatctcga ttgtggtata aaatagattt tatggttgtt tctaactgcc tggtgtaatt
    17161 gcgatcccct gctagtacaa ttgttttgtt catttcattc ccttaatcaa ttcccaagtg
    17221 atagattgca tctaccaata actttttagt gaaatacccc tcttttaaca aatagctaaa
    17281 ggtcttgata cgattcgtca tttcttgata ttcttgtaga ttcattttat caactatctc
    17341 atgaacctct tccagactat ccgccataaa gcctaagcct tgatctacta taaatttagc
    17401 agttgacaag ctacttggta caatgactgg gatgcccgct gtcagatagg tactcacctt
    17461 atgagatata ttcaaggtat agtattggtt actttctccc tcattttgat gggttcccca
    17521 gacaaggcca aatccaccct ttgataattc tagcaacaat tcctcatctt ttttccatcc
    17581 ttcgatgctg agatttctag cactagaact agcttcccct ttatttgaaa atactctcaa
    17641 aggcgtatct tgagaccaat tttgtaagtc tggaaaacgc tctaaacttc cagcaaaaaa
    17701 agttcttttt taaaagcagg ggtgtataag gataaatcat gaggatgatc ccacatccct
    17761 tgaacaagaa tcttcttagt cgtcaatcct tcttccatca ggcgtgtttt cattctctct
    17821 gatggcacta tcaaaacatc tgatagatta tacatgtaca tatagtcttt catgagataa
    17881 tagttactat caaacatgag gggaacaaca tcatggataa agcaaataat tttcacctgc
    17941 atatccttta gcttgtcaaa caagagacga tcaaattcaa agccattcca ggttggagac
    18001 tgaaagacta agatatcacc aatagagata ctggccataa taccatccag acgcttattc
    18061 atttcagaag gactatctga agcaatgttg taaaaataaa taccaacctc tctaaatccc
    18121 agttgactgg ctatcttttg aacagcattt tgagctaaga taaccgtact atcaccagcc
    18181 atgccgtata aatttgttaa atgtaatttc atattatgct atctcatttt ttattactat
    18241 ctacggaagt aaagacaatt tttccatcat tcttctataa atctctgtga actgcatatt
    18301 ttgttcttct aattttgcta tcactctttg taatctattt ttataaatca aaatatgatt
    18361 ggtcaagtca tagtctgaac ttgctaaaat agctatcttt tcttccctag ccccaatttc
    18421 atcatacata cgcttttcac tccatgtatt cgataaagta tcagataaac caacacggta
    18481 ccagtaaaca caacgattca aatagactat cctcgaagct cttagaagta ccttccatgt
    18541 ccagtaagta tcctctgcaa tttttcctat aggaaatggt aaatcttgta gtaactctct
    18601 cttgaataac ttcaagacag ctacagtcca gttcccattt ctgacttctt cgacaccttc
    18661 cctatccata attgcttttc cttctatcac ttccagagaa tcatctggat ccgtaacata
    18721 agtcatatat acatagcgtg tttcatcata agaattataa cgtccgatac taatatctgc
    18781 attttctttt ttcaaagcac catataatcg gtctaaagca tcatgttcca accaatcatc
    18841 cgagtctaca aaagtaatgt acgccccccc cgaacattca atacctaggt tacgagctga
    18901 tgaaagaccg ccgtttgctt tctcaaaata tttgaaacga gaatctttct ctacaaattc
    18961 ttcacatatt ttggatgaat gatctggaga gccatcattg attaataaac actcaaaatt
    19021 ttgatacgtc tgattctgaa tgctatccaa acacattcgc aaataattct caacattgta
    19081 gattggaact acaatactaa ttagttcatt ttccatatac taactttcct agacttgatt
    19141 tattctattt ccctcttaat ctcttctctt tacaacaaga tttccattat tataaactac
    19201 actgtttgat ggaatatcat tgcgtatgag acaacctgct ccaataatgg tattatcccc
    19261 aatcgtcact ccttttaaaa tcgtgacatt acttcctatc cagcagtctc tgccaactct
    19321 aatcggcgct gtagtccact gccacttttc aatcttttca gcagtataaa tatggtcgtg
    19381 atcataaaaa cgaacaccct ctcccatcat ggtaccagct ccgatttcaa ttctttccat
    19441 acagttaaaa ctacaagagt tgttgataaa aacaccatca tgcagtatta acttaccaga
    19501 agagacatga aaattttcaa aatttctaca gatgacattg gctcctacaa tgaagtgagc
    19561 cttatcatcg attttaaaga cattccaagt cccatcttgg atgatttttt cttgatgatt
    19621 ggtgcctact ctgacttcat tcagatagtc tctcactcta gaaaccaatt tgtctggtgt
    19681 acttgataga aaaacttcct gcccctgatt tccatggact gtattgtcaa atgcaaagac
    19741 agattttcca gcctgtacaa acttggagac aatagcatct acttcttgaa agtgattgat
    19801 atctagtaaa aagtcacagg tatcgactag aaagtttagc tgttcttcat ttgtaatcgt
    19861 tcggagacgg acattatagt tcgtttctaa gctacggatt ttctcggaaa attgaacagg
    19921 tgctgcaata tgaaaacaga tatttggcaa ggattcaagg agataatcca actcttcaat
    19981 ctgatcacta accgtcaaaa tcagagcatc tcctactaaa tgatttttct tcatttgctt
    20041 atttctctca atttctcaat cattgcttga acttggtaaa cagcatatgc ctcctgacct
    20101 acttcatagg ttttagtatt ttcaaaggat aagataggct tgccaagatt agcaaattga
    20161 tcgagaattt cttctgtctt ttcgccatga ttaatatcta aaagtacttg actggtttct
    20221 accaattcat tatcgacatc taccaaatag tgaattccgt taaatatagt cacgtttgga
    20281 taaattgtca tctgagccaa tcgatcacta actattactc tagctgctat cttaaactga
    20341 atatcaggca aggattgaac caatgtctca atttgttcaa tatggtctga ggcagtatag
    20401 attagacaag tgaaaggttc ctttattgga tagatgtgag atctttgtaa tggatgtaaa
    20461 tgatggtttt gtcccaattc tgtccattca agcccatgat agtaccacca aacttcacga
    20521 taggtttggg ccgccaaatc tttccacggt ttccgatgag aaagatagtg aataatagca
    20581 ggataatcct gaccctcagg caattgataa tcagcgaact gtttatgaat gacaatatga
    20641 ttataatcaa agtccaattc caaccattta tgttcaaaaa gcatattcaa aatactttga
    20701 tcagcctgtt ccaccttatt atgccattca ttagtcaaat caattaattt ttgggtcata
    20761 ttctcttttt tccaaaaagc attgtttacc aagagaacac cggcattaaa gatttcttga
    20821 ccaaaataag ctctgccccc aaaatctcta acagcagcca aaggataatc ttgtaagtct
    20881 gtagcaaaca aatcatcaag atttttcgtt acaagtaaat cacagtccaa gtagagggcc
    20941 ttatcttctt gcacgaaatc agctatgaaa tagcgtaaaa aaactgtgta actaatatcc
    21001 gttttataac atgaaatttg ctcagaagtt acccgacaat taataatttc tgagtcaaac
    21061 ttctctaagc gcttatttaa ttgcttaatc cattcatttg gaaaatctct atgaatcaaa
    21121 taaaaacgaa gcgagcgatt gtggtaacag atagacctga ttgtcgttaa aacttggtct
    21181 acataggcat aatttgccgc taggacaatg gctttctttt cacttgattc ttgtaccgtt
    21241 aactgattta aaagtctttt tttcatttca aactctttat acattgctgt gtcagataaa
    21301 ccactagctt aaccgttggc gagactgact tccaacatct gacgataaac tgccaagtgt
    21361 ttctctagag gatatcccat attagctagt agcgtaatac gttcagacat agcatcaact
    21421 aaggcgtgca tccacttttc tgtccaaact cttgatagac taccttttct aatccgataa
    21481 gcataaagac ctttatttaa ataaattacc ttttccgata ataaatatac cttttgattg
    21541 aggtaaccat cttctcctaa tttacccatg tcaaagcgca actgctcaaa caatctcgcc
    21601 ttatagagtt taccccaagc agatatcaaa gcgaaactcc tcatttcttg agtttcatac
    21661 aaattctcaa agatggaaac attgtcatat accttctcat aataggagtc tcctgatata
    21721 tgaaagtaga acattccttc actttcgttg aaagaatagt aattcccaac tgcaatatca
    21781 gcttgatatt ctgttatttt tttatataga gtttctacat aatcttgctc aatccaatca
    21841 tccgagtcca caaaggtcac ataatttccg gacatattat tcagaccggt gtttcgtgcg
    21901 gcagaaagac cagcattttc ttgttctata tagagaattc ggtgatccat ttctgaaaat
    21961 tctttacaaa tttcacctga agcatccgta gaaccatcat taacgacaac aatctcaata
    22021 tttttatatg tttgagaaat aatactatct agacatttcc tcagatagtt ttctacattg
    22081 tatactggta caatgactgt tattttatca tccactagca atcttctcct tgatttatgt
    22141 gcgcgagtta gtttagccta actttacagg ttgtcttact cttcttcatc acgtttacgg
    22201 cgtttcgcaa ccaatccaat acctgtaaca gctgctagaa ctccaagtaa cacagatcca
    22261 gttgacgact cagtacctgt attaggcaat tctttttggg atttacctgt cgatccagaa
    22321 gtatttccaa cttgtgagtt cgaatggttt gctgaatttg aaactgatgt acttgctgaa
    22381 gctgaggcac ttgttgacgc tgaagccgac gcactcgttg atgctgaagc cgaggcactc
    22441 gttgacgctg aagccgaggc actggttgac gctgaggccg acgcactggt gcttgctgaa
    22501 gctgaggtgc ttgctgaagc cgattcagac gctgaggtgc ttgctgaagc cgaagcactt
    22561 gttgatgctg aagctgacgc actcgttgag gccgaagctg aggcacttgt tgaggctgag
    22621 tctgaggcac tggttgatgc cgattcagac gctgaggtac ttgctgaggc cgacgcactg
    22681 gtacttgctg atgccgacgc actcgttgat gctgaggctg aggcgctggt acttgctgag
    22741 gctgacgcac tggttgacgc tgattcagat gctgatgtgc ttgctgaagc tgatgcactc
    22801 gttgatgctg aagctgaagc acttgtcgat gctgaggccg acgcacttgt tgatgcggat
    22861 tcagatgcac tggtgcttgc tgaagccgag gcattcgttg acactgaagc cgaggcactc
    22921 gttgatgctg aagctgacgc actcgttgat gctgaagccg aagcactcgt tgatgcggat
    22981 tcagacgcac tggttgatgc tgaagctgac gcacttgtcg atgctgaggc tgaagcactt
    23041 gttgatgctg aagctgaggt acttgttaat gctgaagccg aggcacttgt tgatgctgaa
    23101 gctgaggcac tagt
//
LOCUS       DBERMR010000029        23078 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.029, whole genome shotgun sequence.
ACCESSION   DBERMR010000029 DBERMR010000000
VERSION     DBERMR010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 23078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23078
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.029"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            161..1642
                     /gene="mgaSpn"
                     /locus_tag="ACL8A1_001297"
                     /gene_synonym="mgrA"
     CDS             161..1642
                     /gene="mgaSpn"
                     /locus_tag="ACL8A1_001297"
                     /gene_synonym="mgrA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor transcriptional regulator
                     MgaSpn"
                     /protein_id="HGS1202062.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENRDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVISNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSP
                     YDIIISNFIIPSIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            2004..3005
                     /locus_tag="ACL8A1_001298"
     CDS             2004..3005
                     /locus_tag="ACL8A1_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGS1202063.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     TVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILKKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
     gene            3155..4072
                     /locus_tag="ACL8A1_001299"
     CDS             3155..4072
                     /locus_tag="ACL8A1_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1202064.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAARLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            4083..4973
                     /locus_tag="ACL8A1_001300"
     CDS             4083..4973
                     /locus_tag="ACL8A1_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS1202065.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            5002..6618
                     /locus_tag="ACL8A1_001301"
     CDS             5002..6618
                     /locus_tag="ACL8A1_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS1202066.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVEDLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFSFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYDQYQANKN"
     gene            6628..7947
                     /locus_tag="ACL8A1_001302"
     CDS             6628..7947
                     /locus_tag="ACL8A1_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HGS1202067.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEETGVRQVQNMAFSDDGIHFEKISQNPVATGSDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWREKRFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDTDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(8157..8229)
                     /locus_tag="ACL8A1_001303"
     tRNA            complement(8157..8229)
                     /locus_tag="ACL8A1_001303"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8194..8196),aa:Lys,seq:ctt)
     gene            complement(8494..9765)
                     /locus_tag="ACL8A1_001304"
     CDS             complement(8494..9765)
                     /locus_tag="ACL8A1_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HGS1202068.1"
                     /translation="MPDNLALRMRPKTIDQVIGQDHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYAARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKASGHLPIPRHLRDGHYSGSKELGNAQNYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDHLRKI"
     gene            10035..10274
                     /locus_tag="ACL8A1_001305"
     CDS             10035..10274
                     /locus_tag="ACL8A1_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202069.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAYQEVHSNNGLKKYEK"
     gene            10439..10618
                     /locus_tag="ACL8A1_001306"
     CDS             10439..10618
                     /locus_tag="ACL8A1_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202070.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSGPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            10755..10934
                     /locus_tag="ACL8A1_001307"
     CDS             10755..10934
                     /locus_tag="ACL8A1_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HGS1202071.1"
                     /translation="MPMTQKEMVKLLTAHGWIKTRGGKGSHIKIEKQGERPITILHGE
                     LNKYTERGIRKQAGL"
     gene            10971..11423
                     /locus_tag="ACL8A1_001308"
     CDS             10971..11423
                     /locus_tag="ACL8A1_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HGS1202072.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            11714..12184
                     /locus_tag="ACL8A1_001309"
     CDS             11714..12184
                     /locus_tag="ACL8A1_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HGS1202073.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNAEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDVLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            12203..13291
                     /locus_tag="ACL8A1_001310"
     CDS             12203..13291
                     /locus_tag="ACL8A1_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HGS1202074.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAILFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQFKVRKDVVQTHKAMKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            13272..13406
                     /locus_tag="ACL8A1_001311"
                     /pseudo
     CDS             13272..13406
                     /locus_tag="ACL8A1_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-dihydro-8-oxoguanine-triphosphatase"
     gene            13540..14574
                     /locus_tag="ACL8A1_001312"
     CDS             13540..14574
                     /locus_tag="ACL8A1_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGS1202075.1"
                     /translation="MKKSDVLDLIKYHYEGRETEFRNQSIAIARNFNKHGDTQIAQYI
                     MGLMSQSDRFMPQIENPSEYLTPAKLDIGPLPLPLSIMNDLKGIINAVNHHIGINKFL
                     FVGSPGTGKTESVKQVARLIGKELLVVDFSHLVDSKLGQTVKNLATLFNEINNLPFKQ
                     NYIILFDEIDSIVLDRVNQNDLREMGRVTSAFLKELDRLSPEIVLIATTNLFENLDKA
                     VTRRFDAIIDFDRYTDEDKVEVATIILNELLKQFKNVARDLKLFKKIINSANVIPNPG
                     DLRNSIRTSLAFSDPSDPHDYQKRLLRSLHNGRNLSISKLSKLGFTVREIEILTGISK
                     SSVSRELSED"
     gene            14576..16819
                     /locus_tag="ACL8A1_001313"
     CDS             14576..16819
                     /locus_tag="ACL8A1_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008288790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HGS1202076.1"
                     /translation="MNTLLTLRGKSFTPKSAKGRGPITIPADSIVTLNHLNELYTSLE
                     RVKEYWSNNTIIDGVLISVYYNRVLAKSNRIDGFFNVGGGNAIPNETIVGAKFSNDKS
                     RHIITHYISKEVLDKTIEHTKKVITIFKDYFASAPMTSMVFSDSSKFNQIDYSKYGLS
                     KSKFKQYLRDACFVKGFGIEQADMSTIRNSIVTIYDVKTNLLTLLKQLDIDVSEANIM
                     DQTTVLLDEKNIELLLLKAPYLVSMIVEDFSSLSPENFYNDGNTFYNNLPFPTNEPII
                     GVIDTLFDDRVYFNEWVEYHDLISPDISKGSQDYKHGTAVSSVIVDGASLNPKLDDGC
                     GRFRVRHFGISLQSGFNSFTIIKQIREIVSQNPDIKVWNLSLGSNDEIRENFISVEGS
                     LLDEIQFDNDVIFIIAGTNGIAPDGQRKKIGAPADSLNSIIVNSVDFSNQVVSYSREG
                     VVLSFFIKPDVSYYGGGNGDFINVCEPLGLARVAGTSFAAPFIARKMAYLIHIMGLSR
                     EEAKALLIDSAIPWNDKKTFADISLLGYGVVPIKIEDILSTPDDEIKFIVSDVSRAYE
                     TYNYDFPVPISKEHYPYVAKATMCYFPKCSRNQGVDYTNTEMQLSLGVLDKKGIKTIN
                     NDNQYSENAPGYVREKSARNIFRKWDNVKHLGEKFSSRKKAKPILNPSNPQWGMSVKT
                     IERLRNDDGYGIRFGVVVTLKELNGVNRIEDFIQQAELRGWLVNRLQVEAQVDLFNSL
                     NEEIEFE"
     gene            16870..17889
                     /locus_tag="ACL8A1_001314"
     CDS             16870..17889
                     /locus_tag="ACL8A1_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbiH family protein"
                     /protein_id="HGS1202077.1"
                     /translation="MNITYIVGNGLDLQYGLKTRYKDFYEFQNRTYISRKENEEEYSN
                     LIYESLFSDTVNDYENWSDFELSIGKLTKDNDLISSSIEIKEKFIDDFSEVVDDLREY
                     LRIQQEKNLEKGNAIDFISTLDNMRTSLPVINQPAIDKKYNENLYQHDIVNILTLNYT
                     NVIDKLYNESAKSFSNQLRTNNYKFHIAPPIHAHGTVDICTVLGVSDETQISNSFDEE
                     QKESLIKNLVLKNYRENMDVKNSDIIKNSDIIILYGVSLGETDGYIWNQIAEQSIRSS
                     VPVIIYHYVPHFDAGNPTRVKRLYRNVEDKFIQNSGIDLELEKKLRDNLIVVIGKTIF
                     NLMER"
     gene            17902..18852
                     /gene="prmA"
                     /locus_tag="ACL8A1_001315"
     CDS             17902..18852
                     /gene="prmA"
                     /locus_tag="ACL8A1_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HGS1202078.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDVATVESDLQARLAELTDFMDLGEVKMGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNPG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLTEDAYRLVKDEGYLIMSGIIKDKW
                     DMVRESAESAGFFLETHMVQGEWNTCVFKKTKDISGVIGG"
     gene            18854..19597
                     /locus_tag="ACL8A1_001316"
     CDS             18854..19597
                     /locus_tag="ACL8A1_001316"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HGS1202079.1"
                     /translation="MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDSIIVAYEESAKEGEAAALLQAITGLDTGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     VGLGPRILRAETAPLYALSALSVLVELEK"
     gene            19608..21404
                     /gene="pepF"
                     /locus_tag="ACL8A1_001317"
     CDS             19608..21404
                     /gene="pepF"
                     /locus_tag="ACL8A1_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HGS1202080.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVVADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFITYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDATLTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKHSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIVAYSAELGE"
     gene            21650..22204
                     /locus_tag="ACL8A1_001318"
     CDS             21650..22204
                     /locus_tag="ACL8A1_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HGS1202081.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLFDHLGNPSPEIRNVLLNQGL
                     HYLSKEKDTTGFSSQYGWVHSFAHGADLLTEVVCHPDFPINRVHEVFDILGQLFKRMS
                     IRFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVDFPIEEREDFYKFSNFRSCLVE
                     VYVQLDQRNSLQDELKEAIQSFQY"
     gene            22315..22974
                     /locus_tag="ACL8A1_001319"
     CDS             22315..22974
                     /locus_tag="ACL8A1_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HGS1202082.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLGHLGIAFAFGLAIVVA
                     AYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLGNSGM
                     STASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLSLMA
                     MILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFLGTE
                     E"
BASE COUNT         7345 a         3828 c         4866 g         7039 t
ORIGIN      
        1 tagacggtaa aataagaaaa ttgtttatat attgtctccg tagtgttatt atacgaaata
       61 aaagatttta ggaaaagtcg ttatatcatg ctatacctat tctttgtggt ataattgcaa
      121 gaggtttaat ccgataattt ataaagagag aaagatattc atgagagatt tattatctaa
      181 aaaaagtcat aggcaattag aattattaga attattattt gaacataaac gttggtttca
      241 tcgttctgaa ctagcagagt tattaaattg tacagaacgt gcagtcaaag atgatctatc
      301 ccatgttaaa tctgcttttc ctgacttgat ttttcattct tctactaatg gtatacgcat
      361 tattaatacc gatgatagtg atattgaaat ggtttaccat catttcttta aacattcaac
      421 tcatttttcg attttagaat tcatcttctt taatgaaggt tgtcaagctg agagtatttg
      481 taaagaattt tatatcagtt catcttcgct ctatcgtatt attagccaaa tcaataaagt
      541 gattaaaagg caatttcaat ttgaagtcag tctgacccct gttcaaatca ttggaaacga
      601 gagagacatt cgttactttt ttgcacaata tttttcagaa aaatattatt tcctagaatg
      661 gccgtttgaa aatttttcat cagagccact atctcaattg ttagaattgg tttataagga
      721 aacaagcttt ccaatgaatt tatcaacgca tagaatgcta aagttgctcc tagttacgaa
      781 cctatataga ataaagtttg gtcattttat ggaagtagat aaagattctt ttaacgacca
      841 aagtttggat tttttaatgc aggcagaagg aatagaaggt gttgctcaga gttttgaatc
      901 agaatacaat atctctttag atgaagaagt tgtttgccaa ttatttgtgt cttactttca
      961 aaaaatgttt ttcatagatg aaagtctctt tatgaaatgc gtaaaaaagg atagttatgt
     1021 tgaaaaatct taccatctat tgagtgattt tattgatcag atttcagtca agtatcagat
     1081 tgaaatggag aatagggata atctgatttg gcatctgcat aataccgcac atctgtatcg
     1141 tcaagagttg tttactgagt ttattttatt tgatcaaaaa gggaatacaa tcaggaactt
     1201 tcaaaatatt tttccaaaat ttgtttcaga tataaaaaaa gagctttctc actatttaga
     1261 gactcttgag gtttgttcta gttcaatgat ggtaaatcat ctatcttata ccttcatcac
     1321 tcataccaaa catttggtga ttaatttgct acaaaatcag cctaaactaa aggttttggt
     1381 tataagtaat tttgatcagt atcatgcaaa attcgttgca gagacacttt cttattactg
     1441 tagcaataat tttgaactgg aagtttggac tgaattagaa ttatcaaagg aatctttaga
     1501 agattcacct tatgatatca ttatttctaa ttttattatt ccttctattg aaaataagag
     1561 actcatctat tcaaataata taaacacggt ctcactcata tacttgttaa atgccatgat
     1621 gtttattcga ttagatgagt aaccaaaaat aacaaaaaaa cgctatttcg cgtttgaact
     1681 gtacctcaaa agttagacag aaaaagtcta atttttgggg tgtttttgtt aagaaaagac
     1741 cttcaatcat ttaagcaaaa tgtacatcta gatttatagc cctctaaaac tcttgctttt
     1801 tttgtagaag atacgaaaaa agccatgaaa ccaaggcttt tcctgtttta tgtagatgtt
     1861 tacacatgaa aattgaagta ttttcaaaag ctaattggag tgattttatt catgatggga
     1921 taaatgatct ggccaaaaat gattgagaaa tagttgctta aaaagcattt gcatgatact
     1981 atatattgaa aggaggtgat aatatggtag aacaaagaaa atcaattacc atgaaagatg
     2041 ttgctttaga agcaggagtt agtgttggaa ctgtttcacg tgtaattaat aaagaaaaag
     2101 gcattaaaga agtaactttg aaaaaagtgg aacaagcgat taaaactttg aattacattc
     2161 cagattacta cgctagagga atgaaaaaaa atcgaacaga aacgattgca atcattgtac
     2221 caagtatctg gcatcccttc ttttcagaat ttgctatgca tgtggaaaat gaagtctata
     2281 agagaaataa caaattactc ttatgttcta tcaatggtac aaatagagag caagactatc
     2341 tggagatgtt gcgtcataat aaagttgatg gagtggttgc cattacctat agtccaattg
     2401 aacattactt gacgtcagga attccctttg ttagtattga ccgcacatac tcagatattg
     2461 ccattccttg tgtttcatca gataatgatg caggtggaag agaagcagct aaacaattaa
     2521 ttagtaaagg atgtcagcat ttagcctttg tgggcggaca taatacaacc attaatgaaa
     2581 ccaaacgtcg tagaatttca tttgaaaagt atgtccaaga gcataaaata cctcttagta
     2641 tttttgattt ggatgagaca gttactgact attatggaaa gctagagagt ttcttggaag
     2701 aaaattcttc tatagatgga atttttacaa tcaacgattt tacagctttg gatgtgattg
     2761 aaatactaaa aaaaaatggc aaacgtattc ctcatgatgt ccaaattatt ggatacgatg
     2821 gaattaaaat ggctggagat agagattatc tactttcaac tattgaacaa ccattggaag
     2881 aaatggcaaa agaagcggtt cgtattttgt ttgatataat tgatggaaag actgttaatt
     2941 tgcagacaat cctaccagta aaatttgttg aaggaaaaac aacaaaaaat gaaaataagt
     3001 cttgacagaa agcgctatca atgatagaat gaattcagat aaaaagattt atttttaaaa
     3061 caaaaatgaa acgtttcaaa aaaagaaata aagagacagc gtcaagcgct agcttttcta
     3121 gaaaaaaatg aaacgtttca aaaaggaggt tgctatgaat agcaaagcga agcaagtttc
     3181 tctttgggaa agaatcaaga aacaaaaact cttgttattg atgactgtcc ccggtttagt
     3241 tttaaccttt atctttaaat acatccctat gtatggggtt ttaatcgcat ttaaagatta
     3301 caatccttta aaaggaattt tagggagtga ttggattggt ttttctgagt ttacaaaatt
     3361 catatcctct cccaactttg gtatcttgtt agccaacaca ttaaaattaa gtatctatgg
     3421 tttattgctt ggctttttac caccaatcat tcttgctatt atgctcaatc aactcttgag
     3481 tgaaaaagtc aaaaaacgaa ttcagctcat tttatacgca ccaaacttta tctcagtcgt
     3541 tgttattgtc ggtatgattt tcctcttctt ttcagtggga ggaccaatca acaattttct
     3601 ttctatgttt ggaatgaagg ctgacttctt gacaaatcca gacttcttta gacccttata
     3661 catctttagt ggtatctggc aaggaatggg ctgggcttca acgctctaca cggcaacatt
     3721 ggtaaatgta gatccagcct tagtagaagc agcccgactg gatggagcca atatcttcca
     3781 acgaatctgg cacattgata ttccagctct taagcctatt atggttatcc aatttgtttt
     3841 agctgcaggt ggaattatga atgtcggata tgaaaaagca ttcttgatgc agacatcgtt
     3901 aaatttgcca acttctgaaa ttatctcgac atatgtctat aaagttggtc ttgtatcagg
     3961 agactattct tactcaacag cggttggttt gtttaatgca gtgattaacg tagtattgct
     4021 tgttgcagtt aaccaaatcg ttaaacgcat gaataatggt gaaggaattt aaggagggaa
     4081 gtatgaaaaa ttcgattatg gatacaaaat ttgatagacg tatcttactc ttaaataaaa
     4141 tcattattgt ctttatcgtt ttgatgactt tgcttccttt actttatatc gtcgtagcat
     4201 cctttatgga tcctaaggtt ctggttagta gagggattag ctttaatcca gccgattgga
     4261 ctgtagaagg ttatcagcgt gtattcagtg accaatctat tctaagaggt tttatcaatt
     4321 ctctactata ctcttttgga tttgcagctt taacagtatt gctatctgtg tttacagctt
     4381 atcctctttc taagaaagac ttggttggac gtcgttggat taactacttc ttgattgtga
     4441 ctatgttctt tggtggtggt ttagtcccaa cttacttgct cgtaaaagaa ttgggaatgc
     4501 tcaatactcc atgggctatc attgttccag gtgctgtcaa cgtttggaat attattcttg
     4561 ctagggccta tttccaagga ttgcctgaag aattagttga agctgctgtc attgatggtg
     4621 caaatgattt acagattttc ttcaaaatca tgcttcctct tgcaaaacca attatgtttg
     4681 ttctcttcct ttatgctttt gtaggacagt ggaactcata ctttgatgca atgatttata
     4741 tcaaggatcc aaacttggaa ccattgcaac ttgtacttcg taaaattctc attcagagcc
     4801 aaccaggtca agacatgatt ggagcacaag cggctatgaa tgaaatgaaa cgtttagctg
     4861 aattgattaa atacgcaact attgtcattt ccagcttgcc attgattgtt atgtatccat
     4921 tcttccaaaa atactttgat aaaggaatta tggctggttc acttaaagga taaaaaaaga
     4981 aaaaataaaa ggagttttct catgaaattc aaaacattct caaaatcagc agttttgttg
     5041 acagctagtt tagcagtact tgcagcctgt ggctcaaaaa atacagcttc aagtccagat
     5101 tataagttgg aaggtgtaac attcccgctt caagaaaaga aaacattgaa gtttatgaca
     5161 gccagttcac cgttatctcc taaagaccca aatgaaaagt taattttgca acgtttggag
     5221 aaggaaactg gcgttcatat tgactggacc aactaccaat ccgactttgc agaaaaacgt
     5281 aacttggata tttctagtgg tgatttacca gatgctatcc acaacgacgg agcttcagat
     5341 gtggacttga tgaactgggc taaaaaaggt gttattattc cagttgaaga tttgattgat
     5401 aaatacatgc caaatcttaa gaaaattttg gatgagaaac cagagtacaa ggccttgatg
     5461 acagcacctg atgggcacat ttactcattc ccatggattg aagagcttgg agatggtaaa
     5521 gagtctattc acagcgtcaa cgatatggct tggattaaca aagattggct taagaaactt
     5581 ggtcttgaaa tgccaaaaac tactgatgat ttgattaaag tcctagaagc tttcaaaaac
     5641 ggggatccaa atggaaatgg agaggctgat gaaattccat tttcatttat tagtggtaac
     5701 ggaaacgaag attttaaatt cctatttgct gcatttggta taggggataa cgatgatcat
     5761 ttagtagtag gaaatgatgg caaagttgac ttcacagcag ataacgataa ctataaagaa
     5821 ggtgtcaaat ttatccgtca attgcaagaa aaaggcctga ttgataaaga agctttcgaa
     5881 catgattgga atagttacat tgctaaaggt catgatcaga aatttggtgt ttactttaca
     5941 tgggataaga ataatgttac tggaagtaac gaaagttatg atgttttacc agtacttgct
     6001 ggaccaagtg gtcaaaaaca cgtagctcgt acaaacggta tgggatttgc acgtgacaag
     6061 atggttatta ccagtgtaaa caaaaaccta gaattgacag ctaaatggat tgatgcacaa
     6121 tacgctccac tccaatctgt gcaaaataac tggggaactt atggagatga caaacaacaa
     6181 aacatctttg aattggatca agcgtcaaat agtctaaaac acttaccact aaacggaact
     6241 gcaccagcag aacttcgtca aaagactgaa gtaggaggac cactagctat cctagattca
     6301 tactatggta aagtaacaac catgcctgat gatgccaaat ggcgtttgga tcttatcaaa
     6361 gaatattatg ttccttacat gagcaatgac aataactatc caagagtctt tatgacacag
     6421 gaagatttgg acaaaattgc ccatatcgaa gcagatatga atgactatat ctaccgtaaa
     6481 cgtgctgaat ggattgtaaa tggcaatatt gatactgagt gggatgatta caagaaagaa
     6541 cttgaaaaat acggactttc tgattacctc gctattaaac aaaaatacta cgaccaatac
     6601 caagcaaaca aaaactagag gttgattatg ggagataaga aatacacagt agaaaaagcc
     6661 aatcgtttta tagcagaaaa taaacatctc gttaatactc aatataagcc tgaagaacat
     6721 ttttcagctg agattggttg gatcaatgat ccaaatggat ttgtctattt tcgtggagaa
     6781 taccatctct tttatcaatt ctatccatat gatagtgttt gggggcctat gcactgggga
     6841 catgctaaaa gtaaggactt ggtgacttgg gagcacttgc cagtggcact tgctcctgac
     6901 caagattatg accgaaatgg ttgtttctca ggctctgcca ttgtcaagga tgatcgcctc
     6961 tggctcatgt acactggaca tatcgaagaa gaaaccggtg tccgccaagt gcaaaatatg
     7021 gcattttcag atgacgggat tcactttgaa aagatttccc aaaatccagt tgcaactgga
     7081 tcagacttgc cagatgagtt gattgctgct gatttccgtg atccaaaact ctttgaaaaa
     7141 gatggacgct attactccgt agtagctgcc aaacacaagg ataatgtggg ctgtatcgtt
     7201 ctactagggt ccgataacct agtagaatgg cagttcgaat ccatcttttt aaaaggagga
     7261 gaacaccaag gctttatgtg ggaatgccca gattacttcg agttagatgg gaaagattgc
     7321 cttattatgt cacccatgcg ttatcagcgt gagggagact catatcataa catcaactca
     7381 tcgcttttgt tcacgggtaa ggtagattgg agagaaaaac gttttatccc agaatcagtt
     7441 caagaaattg atcatggcca agacttctat gcgcctcaaa cattgttgga cgatcaaaat
     7501 cgtcgtatcc tgattgcttg gatgcagaca tgggggcgta cccttccaac ccatgaccaa
     7561 gaacacaaat gggcatgtgc catgacttta cctagaattc taagattgga agatggcaaa
     7621 ctaagacaat tccctgttaa aaaaggccaa tatcaaatcc aaatagataa agattgtcat
     7681 taccacttag gaaatgatac agattatctt gaatttggtt atgacagtaa tgcgcagcaa
     7741 gtttacattg atcgtagcca tcttattcaa aaaattctag gtgaagaaga acaggacact
     7801 agtcgacggt atgtagatat tgaagctaaa gaattggaag ttgttctaga taaaaattcc
     7861 atcgagattt ttgtcaatca aggtgaagca agcttgactg caacttatta cttaacggtg
     7921 ccagctgagc tatcacgaat tgattaaaaa ttaagttatt tctcctaaag aaaaagttct
     7981 ctttctaaaa tagtggaaag aggacttttt gtgttttggg tatataagct tagtttatgg
     8041 tatttgtaaa attggtgttg gattatgatt taaactagtt ttctaaagaa tttgaaaaaa
     8101 attttattta agcaaaaaaa gccctatatt ataaggcttt tcctgttgta tttagatgcc
     8161 ccctacaggg atcgaacctg tgacccacgg attaagagtc cgctgctctg ccagctgagc
     8221 taaggaggca aagaaaaaag ctgtattggt gccgaaactt cacggtttgt attgaacccg
     8281 cgcaattaag caggtgggca actcgctcta actgaagctg tttccgtgtg agacggccta
     8341 catgctgtta gaagactttt gtttccctaa taatacaaaa aatagtcggt caacacttaa
     8401 gtgtgaagtc gtacaccaca gcgtttctat atttatatga taccactttt tcaaaaaaaa
     8461 tcaagaggaa agtgcagttt tttgaaaagg atttcagatt tttcgcaaat ggtcgatagc
     8521 ttcctttctt tgagccaaag cccgttcata tttaccagta tcttctgctt ggaaatagtg
     8581 atgattacga attttttctg gcagatagtc ttgcttgacc caatttccag gatagttgtg
     8641 tggatagaga tagttttggg cattccccag ttccttgctt ccactgtagt gcccatcacg
     8701 caggtgtcgc ggaataggca agtgccctga tgctttgagg tcagcaagtg ccttatccat
     8761 agctacatag gctgagttgg attttggaga aagggccaaa tcaatcacga cattggcgat
     8821 gagaatgcgg gcttctggga aaccaatcct ttgtgcagca tccagagcag tcacggtatg
     8881 aatctgggcc tcaggattgg ctaaaccgat atcttcatag gcgataacag tcaagcgacg
     8941 agcgagacta ggcagatccc cagcctcaat caagcgggca gcatagtgga gactggcatc
     9001 cacatctgag ccacggatag acttttgcag ggcagataga acatcatagt gaccgtctcc
     9061 atccttgtcc atagtgatgt agcttctctg aagactattt tccatgatgt ctaaggtgat
     9121 gtggcgaatg ccctcgtcat tctcaggggt agagagaaca gccaagtcca gtgagttaaa
     9181 ggcagagcga aggtctccgt ttgtagaggt tgcgatgaaa tccagcgcat cctcatctag
     9241 ttctattgga aaatcaaaac cacgttcagg gttacttaga gctatctgca gggcctcttt
     9301 gacgtcttgg ttagacagag gttccaactc aaaaatttga acacggctac gaatggcagg
     9361 agtgacggaa aagaaaggat tttcagtcgt tgctccaatc atgatgacca gtccactttc
     9421 caagagaggc aagaggaagt cttgcttggt cttatctagt cgatgaattt cgtctagcaa
     9481 taggacgaga ccaccagaaa atttagcttc ttccgagatt tcttgcagtc gctttttact
     9541 atcaactgtc gcattgaaag ttcgaaaggc atacttggtc gttccagcga tggcagaggc
     9601 aatactggtt ttgccgattc ccggagggcc atatagaatc atggaggaca ggcggttggc
     9661 ttccaccatg cggcggatga tttttccagg tccgaccaga tgatcctgac cgatgacctg
     9721 gtcgatggtt ttagggcgca tgcgaagcgc gagattgtct ggcatagcag tcctttctaa
     9781 cgtggatttg agtagaatga ctcatctact atatcttact atgtccattc tagcctatta
     9841 gttgtgatat ttcaatggtt ttgtaataat cagggagtga gatagaatct tttttactat
     9901 ttaatttaat gaggagaggt atcagcatga gagatttgat tttccttaaa gctcaaaata
     9961 ggatagttga gcctaaagaa gtggaagtgc tgacttgaga aataatattg ctaaatgtca
    10021 ttgtatgaac tataatgaaa agagaggtga taagcaatgg aaatgatgga ccaagtcagg
    10081 aaattttgat ttttacaaaa caaattcgtc attggattct tagtgatcaa gttatttcag
    10141 ggaaaagaaa acttttcttc cgggaagata ctccaaaaga gattttagat ttgtacgaaa
    10201 acattaaatc taaacttgat tttgcttacc aagaagttca ttctaataat gggttgaaaa
    10261 aatatgaaaa atagaataat tgatgttttt gaagtagtaa atcgcctcct tgtaataact
    10321 gttgaaaatc ctgattttga agacttgaga gttaatcata ctcaatgaaa atcaaagagc
    10381 aaactaggaa actagccgca ggctgtactt gagtacggta aggcgacgtt gacgtggttt
    10441 gaatttgatt ttcgaagagt atcagtttgt aaaaattgga gataaaaaat atagagtacg
    10501 cagtggtcct atgattcatt caactccgcc tcaatctgtc ttagatcgag atacttttac
    10561 aattgactat acagatgatg aattgctcga taaagaagca gtttttacta ctcattagaa
    10621 aaataggtct gaataaattg gcttccctta gaaccacatc taggggcttt tatgtataat
    10681 agaataataa gtatcactcc tttacaatac gtgtaacacg tgttataatg gtgttgtcag
    10741 gaggtaaaag cgctatacct atgacacaaa aagagatggt taaactcctt acggcccatg
    10801 gttggataaa gactagaggc ggtaaaggct cccatattaa aatcgagaag caaggggaaa
    10861 gacctatcac aatccttcat ggtgagctga ataagtacac tgaaagaggg ataagaaagc
    10921 aagctgggtt gtaaaattca gtccctgctt tttgatatgg aggtagtgaa atgttagtta
    10981 cgtatccagc tttattttat tatgacgaca cagacggaac agaagcgact tattttgtcc
    11041 atttcccaga ttttgaatac tcagctacac aaggagaggg gatttctgag gctttggcta
    11101 tggggtcgga gtggctaggg ataactgttg cagatttgat agagagtgat ggagagttac
    11161 ctcagccatc agatatcaat agcttgtctt taattgataa tgatcctttt aaagatgatg
    11221 aagatttcgt gtcaacctat gaccttgata aatcttttat ttctatggta tctgttgatg
    11281 tatcagaata cctaggaagt caggaaccca ttaaaaagac tttgactata ccaaaatggg
    11341 cagataagtt gggacgagaa atgggactta acttttctca gactttgaca gacgctattg
    11401 cagataagaa agttcaagcc taagattaag cgtggtatcg tggtaatagt caataaaaaa
    11461 gcacgtccac ttgtgctagt tactcgccta ttgattttat agatttatta gccctttcga
    11521 gggcttttta tattgatttt tatgagaaat gaagaaatca gacttttaag aaaatcactg
    11581 atatcaaggg tttaaatgag gtaatatggt ataattagga cataaaataa ttttgtggta
    11641 agatggtagt atctatttta gcatatttcc gagcaatcgg ggcgattaaa gagtcgcata
    11701 gaaagaggac aaaatggcaa catacggatt tttagatgtt ttagaggaag agttggacaa
    11761 gaactttccc tttgactttg agattagctg ggataaacgc aaccatgcgg tagaagtgag
    11821 ttttctattg gaagcgcaaa atgctgcagg tgtggagatg gtggatgaag acggagaggt
    11881 ttcatcagat gacattcttt ttgaggaagc ggtccttttc tacaatcctg ctaagtcaac
    11941 cgtcaatgca gaagactatt taacggttat cccttatcta cctaaaaaag gattttctcg
    12001 cgagttttta gcttattttg cccttttcct taaagatact gctgaggttg ggctagatgt
    12061 cctcatggac tttttggaag atccagaagc agaggaattt gttatggaat ggaatcaaga
    12121 agtctttgaa gaaggaaaag ttggcttgga agagggagaa ttttatcctt atccaagata
    12181 ctaggagttg gttggaggtg ttatgaagaa aattgggact tatttggttt atgtgctagc
    12241 tgttgtcttt attatgctag cttttgcttg tggaacaatc gcatttgcag agttgggata
    12301 ttccgcagtt ttagccttta cttttggtta tgccttcgct cttctaagca tgtatttaat
    12361 ctttattctt catgagctgg gtcatgcttt ttgtggctac ttgacaggct atcggctggt
    12421 ggcttttgga ttaggacatt ttattttgac caaaaagtca ggcaggtttc atcttagtag
    12481 aacagccatt ctgaaaaatg ttggtgctca atacattggt ttaaaagagg atgaaagcga
    12541 tcaaagaatc atcctgatgc tttcaggagg cttgatggtt catctcggct tgatattatt
    12601 ggcgatattg tttggatttt tgacaagaag ctggtatttt gcagggactt ggatttttct
    12661 taatttatct ttcttcctaa ataacatttt accagtcgac atcaccgatg gagcaaaaat
    12721 ttgggaatta ctacaacacc ctgaaaatac caaatacgcc tacctggtat tgagacattc
    12781 tgcccagacc ttgctggctc ctcaagaata tgatttgaaa gactttgttc aagcagttcc
    12841 tgaggatgca cgagggagtt ttgcggatgg tgtattaggt atgcaaggtg aagtttccat
    12901 tttggaaggg aaggaagaag tagctaaaca gcagtttcaa gcattacttg agaggacaac
    12961 tactccgatg atgcagacag ttgcccaact atccctgctt catatcgcct tgttggaagg
    13021 agattttgag caagcggagc aatatgccag tattcgacag gtcaaatcgt ttctgtctct
    13081 caaactgtcc aatcttcaag tgatacaagc ctggtatcaa tttaaggtaa ggaaagatgt
    13141 ggttcaaact cacaaggcta tgaagattgc tagacagaaa atggatagta gtcgaatgtt
    13201 gagggacgag aaacgctatt atgaaaactt gttagcagag ctggaaaagg aaatgttaga
    13261 aggaactgaa gatggaacta gaaatttctg atttcccagg ctgtaagatt gctttgtttt
    13321 gtggggataa gcttttgact atcttacgcg atgataaggc aagcattccc tgggccaata
    13381 tgtgggaact gccaggtggg ggaaagagtg tcagggaaaa tctgatatag ttttgcatta
    13441 aaagaatatc ctttcaaggt tattcttttt taattttgtc ccaaaataat tgactttgtc
    13501 ccgaaaatat agtattattt gtataaggag gaatgaatca tgaaaaaatc agatgttctt
    13561 gatttaataa agtatcatta tgagggcaga gagacagaat ttagaaatca atccatagca
    13621 attgctcgga acttcaataa acatggtgat acacaaatag cacaatatat tatggggtta
    13681 atgtctcaaa gtgatagatt catgccacaa atagaaaatc ctagtgagta tttgactcct
    13741 gctaaactag atattggtcc tctcccattg cctctttcaa ttatgaatga tttaaaagga
    13801 ataataaacg ctgtcaatca tcatattgga ataaataaat ttttatttgt gggatctcca
    13861 ggtactggaa aaactgaaag tgtaaagcag gtggctagac ttattggcaa ggaattactt
    13921 gtagtagatt tcagtcactt agtagatagt aagctagggc aaacagtcaa aaacttagca
    13981 actcttttta atgaaatcaa taatcttcct ttcaagcaaa attatatcat cctatttgat
    14041 gaaattgatt ccatagtgtt ggatagggtt aatcagaatg atttaagaga aatgggacga
    14101 gtgacttctg cctttttaaa ggagttggat aggctgtcac cagaaattgt attaattgca
    14161 acaacaaatc tttttgaaaa tcttgataaa gcagttacga gaagattcga tgccataatt
    14221 gactttgacc gttatactga tgaagataaa gtagaagtag ctactattat tttaaatgag
    14281 ttattaaagc aatttaagaa tgtagctaga gatttaaaat tatttaaaaa aattatcaat
    14341 agtgccaatg taataccgaa tcctggtgat ttgagaaact caataagaac gtcattggca
    14401 tttagtgacc catcagatcc gcatgattac caaaaacgcc tgctaagaag tttgcataat
    14461 ggtagaaatt tatctatttc taaattatcg aagctaggtt ttactgttag agaaattgag
    14521 attttgacag gtatttctaa gagtagtgta tcacgagagt taagcgagga ttaaaatgaa
    14581 tacattattg acattacgag gtaaaagttt tacaccaaag tcagcaaagg ggaggggacc
    14641 gataacgatt cccgcagatt ctattgttac gctaaaccat ttaaatgaac tttatacatc
    14701 ccttgaacga gtaaaagaat attggtctaa taacacaatc attgatggag tacttattag
    14761 tgtgtattat aatcgtgtcc tagctaagag caatcgtatc gatggttttt ttaatgtggg
    14821 tggcggaaat gcaattccaa atgaaacgat tgtgggtgca aaatttagta atgataagag
    14881 taggcatatt attacacact acatttcaaa ggaagtgtta gataagacaa ttgagcatac
    14941 taaaaaagtt ataacaatat tcaaagatta ttttgctagt gctcccatga cgagtatggt
    15001 gttctccgac tcttcaaaat ttaatcaaat tgattattct aagtatggtc tgtcgaaatc
    15061 aaaattcaaa caatacttaa gagacgcttg ctttgtcaaa ggatttggta ttgaacaggc
    15121 agatatgtct actatacgta attctattgt aacaatctac gatgtaaaaa ctaatctctt
    15181 aacattactt aaacaacttg atattgatgt ttcagaagct aacatcatgg accagacaac
    15241 tgttttactt gacgaaaaaa atattgagtt actactgcta aaagcacctt atctagtatc
    15301 aatgatagta gaagattttt ctagcctgtc accagagaat ttttataatg atgggaacac
    15361 tttttataat aatctccctt ttccaactaa tgaacctatt attggtgtca tcgatacgtt
    15421 gtttgatgat agagtttatt tcaatgagtg ggtagagtat catgatttaa tttcaccaga
    15481 tattagcaaa ggaagtcaag actataagca cggtacagca gtttcctcgg taatagtaga
    15541 tggagctagt ttgaatccaa aactagatga cggatgtgga agatttagag tcagacattt
    15601 tgggatatct ttacagagtg gtttcaattc cttcacaatt atcaaacaga ttagagaaat
    15661 tgtgtctcaa aatcctgata ttaaggtttg gaacctttcg cttggatcaa atgatgaaat
    15721 tagggaaaac tttatttctg tcgagggttc gttgttagac gagattcaat ttgataacga
    15781 tgtaattttt attattgcag gcactaatgg gattgcccct gatggacaac gaaaaaagat
    15841 tggagcacca gcagattcac tcaattcaat tattgtaaat tctgttgatt ttagcaatca
    15901 agtcgtttct tattcacgcg aaggagttgt cctgtctttc tttatcaaac ctgatgtttc
    15961 atactatggc ggggggaacg gagacttcat aaatgtttgt gaaccactag ggctggctcg
    16021 agtagctggg accagttttg cggctccatt tattgctaga aaaatggctt acctcattca
    16081 tattatggga ttaagtcgtg aagaagcaaa ggctttatta attgattcag cgataccatg
    16141 gaatgataaa aaaacattcg cagacatttc tttacttgga tatggggttg tgccaatcaa
    16201 aatagaagat attttgtcta cacctgatga tgaaattaaa tttatagtct cagatgtttc
    16261 tagagcatat gaaacttata attatgattt cccagttcca atttctaaag aacactatcc
    16321 atatgtagca aaggccacta tgtgctattt tccaaagtgt tctagaaatc aaggagttga
    16381 ctatactaat acggaaatgc aacttagttt gggagtttta gataagaaag gcattaaaac
    16441 aattaataac gacaatcaat attctgaaaa tgcaccagga tatgtgagag aaaagtcggc
    16501 acgaaatatt tttaggaaat gggataatgt caaacattta ggagaaaaat tttcttctcg
    16561 taaaaaagct aagccgattc tgaatccttc taatcctcaa tggggaatga gtgtaaaaac
    16621 aattgaacgg ttaagaaatg atgatggtta tggtattcgt tttggagtag ttgttactct
    16681 aaaggagttg aatggtgtca atagaatcga agattttatt caacaagccg aattacgcgg
    16741 ttggctagtt aatagattgc aagtagaagc tcaagtagac ctctttaata gtttaaatga
    16801 agaaattgaa tttgaatagt ggatatttta tccttattct agatcttagg aacacaagag
    16861 ggcaaagata tgaatattac ttatatagtt ggaaatggtt tggatttgca atatggtttg
    16921 aaaacaagat ataaagattt ctatgaattt caaaatagga cttacataag taggaaagag
    16981 aacgaggaag aatattcaaa tctcatttat gagtctctat tttctgatac agtaaatgat
    17041 tatgaaaatt ggtcagattt tgaattgtca attggtaaat taacaaaaga taacgacctc
    17101 atctcctcaa gtatagaaat aaaggagaaa tttatagatg attttagtga agtagttgat
    17161 gatttaagag aatatctacg catacaacaa gaaaaaaatc ttgagaaagg taatgcaatt
    17221 gactttataa gcactctaga taatatgagg acctctctcc ctgtaattaa tcaacctgct
    17281 atcgataaaa aatataatga aaatctatat caacacgata tcgtgaatat attaactctt
    17341 aattacacaa atgtaatcga taaattatat aatgagtcag caaaatcatt tagcaatcaa
    17401 ttacgaacta ataattataa atttcacatt gcgcctccaa ttcatgcaca tggaacggta
    17461 gatatttgca cagttttagg agttagtgac gagacacaga tttcaaatag ttttgatgaa
    17521 gaacaaaaag aatccttaat aaaaaattta gttttgaaga actatagaga aaatatggat
    17581 gttaaaaata gcgatattat aaaaaattct gacatcataa ttctttatgg ggtttctttg
    17641 ggagagactg atgggtatat ctggaatcag attgctgaac aatcaatcag aagttctgtt
    17701 cctgtcataa tttaccatta tgtacctcat tttgatgcag gaaaccccac tagagtaaaa
    17761 cgcttatata gaaatgtaga agacaaattc atacaaaata gtggaattga tttagagtta
    17821 gagaagaaat taagagataa ccttattgtt gttattggaa agactatttt taatctgatg
    17881 gaaaggtaag caaaatacat aatggaaaca tggcaagagt taaaagttac agtgaagcgt
    17941 gagggggagg aattggtttc caatctcttg atcgagctgg gagcgcaagg cgttgcgatt
    18001 gaagacagca tggactatgt gggaaatgtt gaccgcttcg gcgagatttt cccagaggtt
    18061 gagcagcaag aagaaatcgt agtgacagcc tattaccctg atacggttga tgtagcaacg
    18121 gttgagtcag atttgcaggc tcgtctagca gaattgacag attttatgga tttgggtgaa
    18181 gtcaagatgg ggacgactgc cttggctgag gaagactggg cagacaactg gaagaaatac
    18241 tatgagccag ctcgtatcac tcatgatttg accatcgttc cctcttggac agactatgag
    18301 gcgactgcgg gagaaaaaat tatcaagcta gatcctggga tggcttttgg tactgggacc
    18361 catccaacta ccaagatgag cctctttgcc ttggaacagg ttcttcgtgg tggcgaaacg
    18421 gtgctagatg tgggaactgg ttcaggggtt ctctctattg ccagctcgct tctgggggct
    18481 aaagaaattt tcgcctatga cctggatgat gtggcagttc gtgtggctca ggaaaatatt
    18541 gagctcaacc ctggaatgga aaatatccat gtagcggcag gcgatttgct taaaggcgtg
    18601 gagattgaag cagatgtcat cgtggcaaac atcttggcgg atatcctcat tcatctgaca
    18661 gaggatgctt atcgcttggt taaggacgaa ggctacctga tcatgagtgg cattatcaag
    18721 gataagtggg acatggtgcg cgagtcggct gaatcagctg gatttttcct cgaaactcac
    18781 atggttcaag gggaatggaa tacctgtgtc tttaagaaaa caaaggatat ctctggtgtg
    18841 attggaggct agcatgcagc agtattttgt aaaaggcagt gctatctctc ctgtcactat
    18901 cgaggacaag gaaaccagta agcatatgtt tcaggttatg cgcttgaaag aagaggatga
    18961 ggttacctta gtctttgatg atggcatcaa gcgcttggcg cgcgtgctgg atatggaaaa
    19021 tcgtcagttt gagttggtcc aagaattaaa tgacaatgtg gaactaccag tccaagtgac
    19081 catcgcatct ggatttccca agggagacaa gttggagttc atcgctcaaa aagtaaccga
    19141 actgggtgct agccaaatct gggcctttcc tgcagactgg tcagttgcca agtgggatgg
    19201 caagaaattg ggtaaaaaag ttgaaaaact agaaaaaatt gcccttggag cagccgagca
    19261 aagcaagcgt aatattgtac caagtattaa acttttcgag aaaaaagcag attttctagc
    19321 tcagctggac cagtttgact ctatcatagt agcctatgaa gaatcagcta aagaaggaga
    19381 agccgctgcg cttctgcaag caatcactgg tcttgacaca ggagccaaac tgctctttat
    19441 ctttggtcca gaaggcggtc tctcacctgc agaaatcgaa agttttgaag ctaaaggagc
    19501 agttttggta ggcctaggtc ctcgtatttt gcgagcagaa acagcaccgc tttacgcctt
    19561 atcagccctt agtgttttag tagaattaga gaaataagag gaagaaaatg gaacaaaaac
    19621 accgttcaga atttccagag aaggaactct gggacttaac agccctatac caagaccgtg
    19681 aggatttctt gcgtgcaatc gagaaagctc gcgaagacat caaccagttt agccgtgatt
    19741 acaagggaaa tcttcacact tttgaggatt tcgagaaggc ctttgcggaa ttggaacaga
    19801 tctacattca gatgagccat attggcaact atgcttttat gcctcagacg acggactata
    19861 gcaatgacga atttgccaat attgcccaag ctgggatgga atttgaaaca gatgccagcg
    19921 tagccttgac cttctttgac gatgccttgg tggtagcaga cgaggaagtc ttggaccgtt
    19981 tgggtgaatt gcctcacttg acggcagcta ttcgtcaggc caaaatcaaa aaagcccact
    20041 acttaggggc tgatgtggag aaggccttga ccaatctcgg tgaagttttc tatagtccgc
    20101 aggacattta tactaagatg cgagctgggg attttgaaat ggctgacttt gaagcccatg
    20161 gcaagaccta caaaaacagc tttatcacct atgagaattt ctaccaaaat catgaggatg
    20221 ctgaggttcg tgagaaatcc ttccgttcct tctcagaggg acttcgtaag caccaaaata
    20281 cggctgcagc agcctatctg gctcaggtca agtctgaaaa actcttggct gatatgaagg
    20341 gatacgactc tgtctttgac tatcttctag ctgaacaaga agtggaccgt gtcatgtttg
    20401 accgccagat tgacctcatc atgaaggact ttgcaccagt cgctcagaga tacctcaagc
    20461 atgttgccaa ggtaaatggt cttgaaaaga tgacctttgc agactggaaa ttggacttgg
    20521 acagcgccct gaatcctgaa gtgactattg acgatgccta tgatttggtc atgaagtcgg
    20581 tagaaccttt ggggcaagaa tattgtcagg aagttgctcg ttaccaagaa gagcgctggg
    20641 tggactttgc tgctaacagt ggcaaggatt ccggtggtta tgcggcggac ccatatcgcg
    20701 tacaccctta tgtcctcatg agctggacag gccgtttgag cgatgtctat accttgattc
    20761 atgaaatcgg gcattctggt caattcatct tttcagacaa tcatcaaagt tacttcaatg
    20821 cccatatgtc gacctactat gttgaagcac cgtcaacctt caatgaattg ctactcagtg
    20881 attacttaga gaaccagtct aatgacccac gtcaaaaacg cttcgctctg gctcatcgct
    20941 tgacagacac ctacttccat aatttcatca cccacctctt ggaagccgcc ttccagcgta
    21001 aggtgtatac attgattgaa gaaggggaga cctttggagc aagcaagctc aacagcatta
    21061 tgaaggaagt tttgacggat ttctggggag atgctattga aattgatgat gatgcaactc
    21121 tgacttggat gcgccaagct cactactaca tgggcttgta tagttacact tactcagcag
    21181 gactagtcat ctcgactgct ggttaccttc atctaaaaca ttctgaaact ggagctgaag
    21241 actggctcaa tctccttaaa tcaggtggta gcaagacacc gcttgagtca gccatgatta
    21301 tcggtgctga tatttcaaca gacaaaccac tccgtgatac catccaattc ttgtctgaca
    21361 cagttgacca gattgtcgcc tatagtgctg agttgggaga gtaagggaat agaaaaattt
    21421 tttagaatga tgtttatggg cttgttaagc gtcattctat ttgtcttagt tgtagctatt
    21481 ttcaaaaatg tcgttgaagg tcgtgatgtt aaatttgaac ttactgtaga actggtattg
    21541 atagttattt acgtcatttt atatcagatt gtatttaatt gagaattcaa tattgagtca
    21601 tttaagactg cctttttgaa cagttgctag aaatgtagag gtattgacca tgtatcaaga
    21661 tttactaaga aaaatagcag aagaaaaacc aaattataac caggaagaaa tccagtggtt
    21721 gtttgatcat ttgggaaatc cttctccaga aattcgtaat gtccttttaa atcaaggctt
    21781 gcactatctt tcaaaagaaa aggatacaac aggtttctca agtcagtatg gttgggttca
    21841 ttcttttgca catggagccg atttactgac agaggtggtt tgtcatccag actttcctat
    21901 aaacagagtt catgaagtat ttgatatact tggccaacta tttaaaagaa tgtcgattcg
    21961 ctttacagat gatgaggatt ggcgtttagc aagagtgatc tatgaaccta ttttacaagg
    22021 gaagttggag caagaacaag tagcttcttg gataaaaact gttgattttc cgatagaaga
    22081 aagggaggat ttttacaaat tttccaactt tagatcctgt ctggtggaag tctatgtcca
    22141 acttgaccag agaaatagtt tacaagatga gttgaaagaa gctatccagt cttttcaata
    22201 ctagagatta gctaatagtt ctatgatttt caaaaaattt ccaatcaatt ttcttgaaac
    22261 cctttcctac ttttgataga ctagtatcta gtttttgaaa aaaggagaaa gaaaatgaaa
    22321 aaatttgttg ctgagttaat cggtacgttc atgcttgtgt tcgttgggac aggagctgtt
    22381 gtttttggaa atggccttgg tcaccttgga atcgcctttg cctttggttt ggcaatcgtg
    22441 gtggcagcct actcaatcgg aactgtttca ggtgctcact tgaacccggc tgtttcgatt
    22501 gctatgtttg taaacaaacg tttgtcatct tcagaacttg taaactacat ccttggtcag
    22561 gttgttggag ctttcatcgc ttctggcgca gtcttcttcc tcttgggtaa ttcaggtatg
    22621 tcaactgcta gtcttggtga aaatgccttg gcaaacggtg tcactgtctt tggtggtttc
    22681 ttgtttgaag tcatcgcaac tttcttgttt gtcttggtta tcatgactgt gacttcagaa
    22741 agcaagggca atggcgcgat tgctggtttg gtaatcggtt tgtcattgat ggcgatgatt
    22801 cttgtcggat tgaagattac tggactttca gtaaacccag ctcgtagctt ggcaccagct
    22861 gtcttggtag gcggcgcagc ccttcaacaa gtttggattt tcatccttgc accaatcgct
    22921 ggtggagttc ttgcagccct tgttgcaaaa aatttccttg gaacagaaga ataattgaaa
    22981 ctcaaaaagc cttgctcctc atcttgagga acagggcttt ttcgtatgat actcttcgaa
    23041 aatctcttca aactacgtca gcttccatct gcaacctc
//
LOCUS       DBERMR010000030        23075 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.030, whole genome shotgun sequence.
ACCESSION   DBERMR010000030 DBERMR010000000
VERSION     DBERMR010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 23075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23075
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.030"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(1..384)
                     /locus_tag="ACL8A1_001320"
                     /pseudo
     CDS             complement(1..384)
                     /locus_tag="ACL8A1_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(446..895)
                     /locus_tag="ACL8A1_001321"
     CDS             complement(446..895)
                     /locus_tag="ACL8A1_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HGS1202083.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(897..1655)
                     /locus_tag="ACL8A1_001322"
     CDS             complement(897..1655)
                     /locus_tag="ACL8A1_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HGS1202084.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(1770..3749)
                     /gene="stkP"
                     /locus_tag="ACL8A1_001323"
     CDS             complement(1770..3749)
                     /gene="stkP"
                     /locus_tag="ACL8A1_001323"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase StkP"
                     /protein_id="HGS1202085.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSAE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(3746..4486)
                     /locus_tag="ACL8A1_001324"
     CDS             complement(3746..4486)
                     /locus_tag="ACL8A1_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HGS1202086.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDAE"
     gene            complement(4501..5814)
                     /gene="rsmB"
                     /locus_tag="ACL8A1_001325"
     CDS             complement(4501..5814)
                     /gene="rsmB"
                     /locus_tag="ACL8A1_001325"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HGS1202087.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLSAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIANIKRKNKRDSIAYSLPVWLVTKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVADRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(5807..6742)
                     /gene="fmt"
                     /locus_tag="ACL8A1_001326"
     CDS             complement(5807..6742)
                     /gene="fmt"
                     /locus_tag="ACL8A1_001326"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HGS1202088.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGALFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(6755..9151)
                     /locus_tag="ACL8A1_001327"
     CDS             complement(6755..9151)
                     /locus_tag="ACL8A1_001327"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HGS1202089.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNPQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVVKRAYEVMNILRSSLSETSSILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(9217..9531)
                     /gene="rpoZ"
                     /locus_tag="ACL8A1_001328"
     CDS             complement(9217..9531)
                     /gene="rpoZ"
                     /locus_tag="ACL8A1_001328"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HGS1202090.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(9556..10182)
                     /gene="gmk"
                     /locus_tag="ACL8A1_001329"
     CDS             complement(9556..10182)
                     /gene="gmk"
                     /locus_tag="ACL8A1_001329"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HGS1202091.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(10312..11925)
                     /locus_tag="ACL8A1_001330"
     CDS             complement(10312..11925)
                     /locus_tag="ACL8A1_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HGS1202092.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(12116..12370)
                     /locus_tag="ACL8A1_001331"
     CDS             complement(12116..12370)
                     /locus_tag="ACL8A1_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HGS1202093.1"
                     /translation="MLLKFTEDAWADYCYWQNQDKKTLKRINKLIKDIQRDPFTGIGK
                     PEPLKYDYQGAWSRRIDAENRLIYMMDGDSVAFLSFKDHY"
     gene            complement(12374..12628)
                     /locus_tag="ACL8A1_001332"
     CDS             complement(12374..12628)
                     /locus_tag="ACL8A1_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HGS1202094.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMAQVRAGSTQVHVIEE"
     gene            complement(12866..13963)
                     /locus_tag="ACL8A1_001333"
     CDS             complement(12866..13963)
                     /locus_tag="ACL8A1_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HGS1202095.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVNILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGAEMEKFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLSYILVQARESNLPEAIHVLGFTEKGRQHLKSLKGQVSLVSRIGKEPWDA
                     MTQKADQIYQLGKPSIAEQNFGRVPIRIETN"
     gene            complement(13973..14713)
                     /locus_tag="ACL8A1_001334"
     CDS             complement(13973..14713)
                     /locus_tag="ACL8A1_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGS1202096.1"
                     /translation="MATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLELA
                     CGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGKYD
                     FVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHENA
                     EDFAMLWDTYEDEVPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQAG
                     FKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            complement(14822..15175)
                     /gene="rsfS"
                     /locus_tag="ACL8A1_001335"
     CDS             complement(14822..15175)
                     /gene="rsfS"
                     /locus_tag="ACL8A1_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HGS1202097.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            complement(15188..15688)
                     /locus_tag="ACL8A1_001336"
     CDS             complement(15188..15688)
                     /locus_tag="ACL8A1_001336"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HGS1202098.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLGKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            complement(15689..16282)
                     /gene="yqeK"
                     /locus_tag="ACL8A1_001337"
     CDS             complement(15689..16282)
                     /gene="yqeK"
                     /locus_tag="ACL8A1_001337"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HGS1202099.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLDLIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKEN"
     gene            complement(16282..16911)
                     /locus_tag="ACL8A1_001338"
     CDS             complement(16282..16911)
                     /locus_tag="ACL8A1_001338"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HGS1202100.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRAFLAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            complement(16963..17274)
                     /gene="yhbY"
                     /locus_tag="ACL8A1_001339"
     CDS             complement(16963..17274)
                     /gene="yhbY"
                     /locus_tag="ACL8A1_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HGS1202101.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            complement(17489..18595)
                     /gene="yqeH"
                     /locus_tag="ACL8A1_001340"
     CDS             complement(17489..18595)
                     /gene="yqeH"
                     /locus_tag="ACL8A1_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HGS1202102.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGIAKVAVWAPEGVAVVTRKAII"
     gene            complement(18598..19125)
                     /locus_tag="ACL8A1_001341"
     CDS             complement(18598..19125)
                     /locus_tag="ACL8A1_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HGS1202103.1"
                     /translation="MAIENYIPDFAVEAVYDLTVPSLQAQGIKAVLVDLDNTLIAWNN
                     PDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVEKFGIDYVYWALKPFTFGIDRA
                     MKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKPLVQHDSIKTQINRTRERRVMR
                     KITEKYGPITYKKGI"
     gene            complement(19140..20048)
                     /locus_tag="ACL8A1_001342"
     CDS             complement(19140..20048)
                     /locus_tag="ACL8A1_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HGS1202104.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            complement(20105..20665)
                     /locus_tag="ACL8A1_001343"
     CDS             complement(20105..20665)
                     /locus_tag="ACL8A1_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HGS1202105.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            20804..22015
                     /gene="sstT"
                     /locus_tag="ACL8A1_001344"
                     /pseudo
     CDS             20804..22015
                     /gene="sstT"
                     /locus_tag="ACL8A1_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            22083..23036
                     /locus_tag="ACL8A1_001345"
     CDS             22083..23036
                     /locus_tag="ACL8A1_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HGS1202106.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
BASE COUNT         6186 a         5446 c         4540 g         6903 t
ORIGIN      
        1 ccttgataac cactgtcagc caagatttta ccagcttgtc cgatatttct gcaactcatt
       61 ttgaacaact tcatatcatg acaatagttc acagtgatat ccaaagaaac aattctccct
      121 tgacttgtga caatcgcttg agtcttcata gcgtgaaatt tctttttacc agaatcattc
      181 gctaattctt tttttagggc gattgatttt tactttcgtc gcatcaatca ttaccgtgtc
      241 ctcagaactg agaggagttc ttgaaatcgt aacaccactt tgaacaagag ttacttcaac
      301 ccattggctc cgacggagta agttgctttc gtggatacca aaatcagccg caatttcttc
      361 ataagttcga tattctcgca catatagaaa gcgttgttaa tttatttatc tcatttttca
      421 gaaaattctt ttatttctgt aaagtctacg atactcgatg tgtttttata taatgataca
      481 gtctgagaat cactgttccg ctagccattc caatagagat taccagagcc aacatgacaa
      541 ccaaggtcgc acttgccagt gctttattat agtcccctgt cacaaaaaag gcagttgttc
      601 ggtaggagag ataacctgga accagcggtg ccaaaatggc caagataaag accacagcag
      661 gtgtcttata aagaatactt aaaatctggc tgacacaaga accaataatg gctgcaatga
      721 aggtagctac aatgacattg gtcggttcct tgagcaagag atagattagc cagacagtca
      781 tgcccaaaat ccctccaggc aagagcatag accgttgcac attgagtacg attaaaaaag
      841 tgataatggc aagaaaactt gctactgctt gtaataaaaa ggttgttagt gtcatattag
      901 ttcatcaata ccaaggcgac agaagttcct gcccctaaag cgagggtaat gagcagggat
      961 tcaaacatct tactcatacc agagtttatg tggttggtca taatatcacg gaccgcattg
     1021 gtcaaggcaa tacctggtac aaacggcatg accgcaccag ctataatcaa atctgccgtt
     1081 gaaggaaaac ctgtgtagcg agcccaaaac tgggcaatta tcccaaagac aaaggctcca
     1141 gcaaaggctg tcacaaaggg aattcggata aatttttcca catagaggga aaaggcaaaa
     1201 ccaaataagg tcgccactcc tgccccaagt gcgtcgtaga tatttccgct aaacataact
     1261 gaaaagaaag gagcactaaa ggtcgcagcc agagttacct gcaacttagt atagggaagg
     1321 ggttgagctt gcaaggccgt caattgctta aaggctgttt ctaagtcaat ctgcccccca
     1381 actagctgac gagaaatctg gttcacatcg cagacttttt cgatgttata agaagaggag
     1441 gtcacgcgct tcatgcgcga aatattggta ttttcaatag agaaaaagat agcggcaggc
     1501 atggcaagga cattgcaatc cacaatcccc tgcgaatgcg cgattcgaat catggtatct
     1561 tctacacgat ggatttctga gccactttta aggagaatag tccccgctag cataatcaca
     1621 tcaatgacgg catttaattc ttttgattct tccatgcttt cctcctttta tcaactccct
     1681 ctattctatc acaaatctgg actcaaaaaa aatctttgcc atgaaatcat gacaaagatt
     1741 gattactcat tttgattatc catctgcttt taaggagtag ctgaagttgt tttaggtttg
     1801 tagattgaaa tcttgactct agtcttattt aggtctacct tttcacctgc tctaggactt
     1861 tgttcaacaa ccatgccttc tgcactacct gcaggcgctg tcgtcacttc tacaacttct
     1921 atattagctt ccttaatccc aacaatttga atcaaattgt tcttagtaaa ctccaagcta
     1981 gaaccaatgt aactcggcat ggcaacactt gtaacttttt tagctactgt caagacaatt
     2041 tgagtaggtt tactcacatc ataagtcgtt ccggcacctg gactttgttt cataatcgtt
     2101 cctggttcgc tttcgctgga ctcttcttcc tctatcttaa tcaaattctc aggaaccttc
     2161 ttctgcttga gttctgagat tacttctgta gagttccgtc caatatagtt ccctaattga
     2221 atcgtcgtag cttttttagc tactgtcaaa acaatttgag ttgccttgct caagtcatag
     2281 gtcgtacctt ctggtagact ttgcttcagg accgttccag cctcactctc attcgactct
     2341 tcttcctcaa ttttaatcaa attatctgga acttttttct cttttaattc cgcaatgaca
     2401 tcagaggatt tccgaccgac ataattacta atttggaaag attgcttgcc tgatgagaca
     2461 accaaattga ttttcgttcc ttcttttcga ccagttccag cgccaggatc tgtacggata
     2521 atccgccctt cttccacctt ttcactagcc tctgtcttct cctcaccaat ctcaaaattg
     2581 gcttttttga gcgttgcctt ggcctctgca actgtctgac ctgccacatc tggaatggca
     2641 atggttgcag gagttctgga taatatccaa ataagagaag ctgccaccaa tacaaggctg
     2701 gccaacaaaa tcaggtaacg catcttaaat ctatgttttt tcggtgcttg tggttggtaa
     2761 gtttcctctg tcacagcctg gcttgggttt ttgattgatt tgtgttctgt ctgcgcttga
     2821 accttaggaa tagatgtcaa ggtactctga gaaaccttcg gcaaggtctt ggtatctgcc
     2881 ttgctcgttt catcaaagat taacttactt tcatttctac gattgtagga caagctacta
     2941 gacaagtcca catacatctc tgaaaccgag cggtagcgat tggtcaactt tttagcagtt
     3001 gccttgataa taacattttc taaagcctga ggtacagatg gattttctgc aataacggac
     3061 ggcaggggtt tctggaaatg ctggagggca atggtcaccg cgctatcccc gtcataaggg
     3121 atatggcctg tcaacatctc atagaaaata atccccatgg catagatatc actctgcaca
     3181 gtcgccttcg aaccacgcgc ctgctctggt gacaagtaat gaactgagcc caacatcgag
     3241 ttagtctggg tcagacttgt ctctgcaaag gctacagcaa tcccaaagtc tgtgaccttg
     3301 gcagtcccat ctggtgtcaa aaggatattt tgaggtttca agtccctgtg aacaattcct
     3361 cgagtatggg ccaagcgcat agccaagaga atttgtccca tgatacggac ggcttcttca
     3421 ttagaaagag gataatgttc cttgatatag cgtttgaggt ctagtccagc aacatactcc
     3481 attgcaagat actgttgacc gtcttcctca ccaatatctg ttatccgaac gatatgagga
     3541 tggtctagat ctgccatagc tctcgcttca cgctgaaaac gagctacagc tatcgggtcc
     3601 gtctggtagt tggtcctcag aaccttcact gccacttctt ccccatctaa gattaagtct
     3661 ttggctaggt agacatccgc catgcctcct cgaccaatct gtttgacaat ccgatagcgt
     3721 ccggcaaaaa tcttgccgat ttggatcatt ctgcatcctc ctcgttcata gaaacaaggg
     3781 caaccgtaat gttgtctaaa cctcctgcat tgttagcaaa acgaacaagt gtctccgttt
     3841 tatctgctaa aggaatatca ctggttacaa tatcacgaat ctcactgcct gaaatcatgt
     3901 tggtcaagcc gtcactattg agcaagagat agtcacctga ctcaaggata actgtcccaa
     3961 aatcaggctg aatttcatct ttttgcccaa tagactgggt gataatattt ttttgcggat
     4021 gagcttctgc ctcttctggt gtcaattgac cagccttgag caattcatta accaaggaat
     4081 gatcgctcgt caactgatgg tattcttctc cacgaatcaa gccgatacgc gaatcaccaa
     4141 tatgagcata gatagcctga ttatcaataa tagcaaggac ttccaaagta gttcccatgc
     4201 ctctgtaagc ttcatcctga ccaagctggt gaatcttttg attttcaatt tctaggtaat
     4261 gggcgaacca ttcacgcact tcattgactg tatcgatctg ggtatcaacc caagctacac
     4321 ccaggtctgt caccgccatt tcactagcga tattccctgc gcgatgacct cccatcccat
     4381 cagctaaaat aatcatggta cgtccagctc tattgacata gtggttgaca tagtcttggt
     4441 tatttgttcg tttctgacca acatctgtta ataatgaaat ttccatgtgt cagttccttc
     4501 ctaatccgat atcttgcgaa attgactgat gaagaatcca tcacttccat acaattcagg
     4561 tgtaatgagg atacagccgt ctttcatgat atccttacat tcatgttcta gttttacctg
     4621 ctcgaactcg ggatgactct ctaaaaaggc cttaacgact tgaaaattct cctctgagac
     4681 gatagtgcag gtgctataag ttattatacc acctttgcct agtatttgac aaacactacc
     4741 taatatttct aactgaattt cctgcaagga cgcgaaatct gccgtttctt tattgtattt
     4801 gatatctggt tttcggcgca aaagaccgat tcctgaacaa ggagcatcca ccaaaatctt
     4861 atcaaaggaa tcctggtcaa aaaactcatg cacctttctg gcatccaatt tttgagtttg
     4921 aacccgatct gcaactccca gacgttgggc attttcttga attaaatcca acttgtggtc
     4981 gtacaagtcc agagcagtaa cctgacctgt cgtaagataa gaggctatat gggctgtttt
     5041 cccacctgga gccgcacagg catcaagcac tcgctcatca ccttgtaaat caagcgtcgg
     5101 agcaaccagc tgactggact cgtcttggat ggtaatggct ccatccgcaa acaaattatg
     5161 ccctgcaaaa tgcccctgct ccttaaccag accagtggtt gctaaaaggg aattattcgc
     5221 ctccaacaag gcttggattt cctcttttcg acttaggtct gttacacgaa tactagcttt
     5281 gtttcgcact aacaggcttt caaagatggc ttttgctctc tcctctccgt attcttcctt
     5341 gagtttggta actagccaaa ctgggagaga ataggcaatg gagtcacgct tgttttttcg
     5401 cttgatgtta gcaatatctg gccagccttc acgcaagata cggcgaagga cagcgttgac
     5461 caatttttca ctgccttttt tacggagttt ggccaattcc actgcttcat tgaccacagc
     5521 atgatctgga atcttgtcca aatagcggag ttggtaggca ctcatgagaa gaaggacata
     5581 gagccagctg tctaactggt ctctgtcttc gataaagtgg gataggtacc attccagagt
     5641 cagtttacgg gctaccgttc catagactag ctcggtcact aagcccttgt ctgctgcaga
     5701 gagctgactc cccttgaggt gtttatttaa ggcgatattt gaatatgctt ggttcacaaa
     5761 aacatcctct agcactgcta gagctaaact tctagccgtt tctactttag tcaccaaatc
     5821 gttctcctac agtcaatgta cgtccaactc cgttgaggaa ggaagcaatg tccatcttag
     5881 gcttaccagc tggctgcact tgtttgaggg atagagcccc ttcagccgtt gcgacaatca
     5941 attctttctt gccgatagag agaatctcac ctggatttcc ctgaccttct actggtaggg
     6001 cttcataaat cttaaagcgg tcgcccttaa ggaaagtatg ggcaacaggc caggggttca
     6061 ttccacgaat ttggttaaag agttgacgat tggttttgtt ccagtccagt ttttcttcct
     6121 ctggctttat atttggagag aaggtaacct gactcgtatc ctgcggttca ggtttgatat
     6181 caccagcaat ataggcaggc agagtgtcca aaagcaaatc acgaccaact agcgccaatt
     6241 tttcaaacaa ggcgccaaca ttgtcctcat ctgtgatcgg aatgctgcga cgagaaatca
     6301 tatctcctgc atccatttcc ttaaccattt ccatgatggt cacaccagct tcctcatccc
     6361 cttgaatcaa ggcataatgg ataggcgcac caccacggtg tctaggaagg agggaggcat
     6421 gaacgttgac agcaaagtcc atgctatcaa ggagtttgct tgggagaaac tgcccaaaag
     6481 cagcagtcac aattccatct gctcctagct tcataagatc ttccatctct ggacttccag
     6541 ataatttttc aggttggtag atagatagtc ctgcttcctt tgcagcctgc ttgactgggg
     6601 tttcttggat aactttttta cgaccaacag cacggtctgg ctgggtcaca acggctagaa
     6661 tttcgtaacg gtcatctgtc aaaagtcctt ttaagactgt tgctgaaaag tcgggggtcc
     6721 ccataaagat tagttttgtc atatcttctc cttcttataa aaattgctgc ggctcatggt
     6781 caatgctgag acggagctca ctattttccc gttcttgagt caaggccagg acctggttga
     6841 gggtcggccc cagctcatct tctaaacggt atttaattaa aatctggtaa tgatagaggt
     6901 tgtgggtacg ggcaatcggt tttggcgttg gccccagaat ggaactggtc tctgacaagc
     6961 ttgaccgcaa aatattcatg acttcatagg cacgtttgac cacctcttct tctttcttgt
     7021 gagaaagggt aataccaatc gtgaaatagt aaggtggata gccgagttgt cgtctgattc
     7081 ccatttcata ggcataaaag ccttcgtaat cttgatcctt ggcaaatcga atagcatagt
     7141 gctgcggatt gtaggactgt atcaagactt gacctgcctt ttcagcccga cctgctcgac
     7201 ctgccacctg agtcaagagc tggaaggttc tctcagaaga acggaaatca ggcagattca
     7261 aggccgtatc cgcatttaga actccgacta gggtaacatt gggaaaatcc aacccctttg
     7321 caatcatctg agtaccaagt aaaatatccg cttcccctcg cccaaactgg tcaagcaagg
     7381 cttggtgact gcctttcttt cgagtcgtat ccacatccat cctcagaatg cgagcttggg
     7441 gaaagagttc tgctagctca tcataagcct tctgagttcc cgtcccatag taacgaatac
     7501 tgcggctctt acagttagga cagacctgag gaatatcctt cgagaaacca caataatggc
     7561 agttcatagt cttggtatcc atatgcaagg tcagagaaat atcgcagttg ggacaagtat
     7621 ccaccgtccc acactcccga cacatgacaa agctagaata accacggcga ttgagcatga
     7681 gaaccacctg ctctttttta accagacggt cttggatagc ctctagcaaa ggaggcgtaa
     7741 agtttgacgt ctcattttgt ccgatatagt ctcgaaagtc aatcacttga acctcaggga
     7801 ttgtagccaa aggattggca cgttgggtta gacgtaagtg ttgatagacg cctttgccag
     7861 cacgtgcccg gctctctaag ctcggcgttg cagatccaag taccagagtt gcttgattat
     7921 actgagcccg taaaatagct acctctctgg catggtaacg gggattgctg tcctgcttat
     7981 aagccgcttc atgctcttca tcaataatca tgacacccag atttttcaga ggagcaaaga
     8041 tagcagatct ggcaccaaca acaacttggg catcgccacg ctccaccttg cgccattcat
     8101 catacttttc accattggat aatcctgagt gaagaatggc taccttgtcc ccaaaacgtg
     8161 ctataaaacg ctcggtcatc tgaggagtca aggaaatctc aggtaccagc aaaatagctg
     8221 tcttgccctt atccagggca ccttggataa tctgcaagta aacctcggtc ttcccacttc
     8281 ctgtaatccc ttgaagtaga aagggaggtt gagaactgcc aatagaactc acaaccgcat
     8341 cacgcgcctg tctttgttct ggatttaact ccaaaggtct acttgcttca attccttcaa
     8401 aataagcagc cgagcgttga acttcctttt ggactatggt aacagcacct tgatccacaa
     8461 agaagttgac ttgctctcgc gagtaggact ctaacaagct agccaaggaa gcgctctctg
     8521 gatgagacag cagataatct ctcagttcca actttttctt ggcacgtgta gaaatctcaa
     8581 caccttctaa ttgagcatgg tcaacctcat accaagactg ggtcttgacc ttcttttgat
     8641 cgactgcctg atattccaga ccaagcaggc cttttctagt caaacgcatc atttcagctt
     8701 gcttggcaag gtctagtgaa gaaaaggcta gcgaatcttc tgaaccaaac aggcgcactc
     8761 gttcttcctg actcaagcct tccagaggat agagaatctt gtcatagcta gaattcagaa
     8821 atcctggaag catggccttg aggatagaaa ttttgtagga gaagacagat ttgcgtaact
     8881 cctcagccag ccagagttgt tctggcgtga gaacaggaga aaaatccagc acctctgcaa
     8941 tatcttttaa atcttgctcc atctcttctc catctgattg ggactccaaa ccaagaacaa
     9001 tcccttgaat caggcgatta cccttaccaa aaggcacatg aacccgcatc ccaacttcca
     9061 gcattccctc aaattcctcc ggaatcctgt aactataggg ctggtccgtc tgcatcaagg
     9121 gcacatctac gataatctta gctagggcca tcttctcacc tcctccttgt cagtacattc
     9181 ttgcaataga aaaaataaga ttgagtcccc ccaaccttaa attttttcac catcttcttt
     9241 ttctttagca atttgctctt tgattttctt ttcttcttct tctttgcggc gtttttcttc
     9301 ttcgatacgg cgacgcactg cttcacgttt tccttctgga tctgggtgaa ttgtaacgtt
     9361 tcctgattcg atttcttcta aagcgcgaag agttgatttt tcagacttga aaccttgagt
     9421 tgctggggca cctgcttcca attcgtgggc acgttttgct tccaagatta cgagtgaata
     9481 ttttgaagga accttgtcga gcaaggtatc aatagagggt tttaacatca tttgcttgta
     9541 cctattttct aaattttatc gggtagttgg agattttggt aacatctcct gatagtgacc
     9601 aatgacacga tccacacaga agtgttctgc ttcaatcaca catttgacac gttcagcagc
     9661 taggggcacc tgatcgttga caatcgcata atcatactca cgcatgaggg caatttcttc
     9721 cttggccttt tcgattcgtt gggcaatcac ttctgcacta tctgttccac gacctaccaa
     9781 gcgatcttgc aattcatcca aatctggtgg tgtcaggaag ataaagacag catctggaac
     9841 ctttttcttg acctgaagag caccctgaac ttcaatttca aggaaaacat cgattccctt
     9901 gtccaaggtt tcattgacat aggtcagagg agttccatag tagttgccga catattctgc
     9961 gtattccaac atctgtcctt gacgaatcag ctcttcaaat tcttcacgag tacggaagaa
    10021 atagtcaaca ccgtccactt ctccaggacg ttgtgcgcgt gtcgtcatcg atacagaata
    10081 ttgaaattgg ttttcagaac tctcaaaaat ctctcttcta accgttcctt ttccaacccc
    10141 tgaaggacca gaaaaaacga ttagtaagcc tcggtctgcc attgtgtctc cttttagtca
    10201 atctgtgaaa taacatttct ctagaataat ggcaaaaagc cagattatcc tttacagtct
    10261 ttctatctag tgtaacaaaa aagcagtaat ttttcaactg ctctttctta tttatttagc
    10321 ataatctact gcacgaagct cgcgaatcac ggttaccttg atatttcctg gataatcgag
    10381 attgttttca attttcttac gaactttgtg agccaagatt gtgactttgt cgtccttgat
    10441 ttttcctgga ttgaccatga tacgaatttc acgtcctgct tgaagggcaa agctagtttg
    10501 cactccttca aagccgttag caatttcttc caaatcatgg agacgcttga tgtagctttc
    10561 aagagactca ctacgagcac ctggacgggc tgcgctcaag gcatctgctg cagcgacgat
    10621 aactgctatc acgctctcag cttcaacatc tccgtggtga ctagcaatcg tattcaccac
    10681 aactgggggt tccttgtact tacgggccaa ttccataccg atttcaacgt ggctaccttc
    10741 aacctcatgg tcaatggctt tcccgatatc gtgaaggaat ccagcacgac gggcaagagc
    10801 cgcattttca ccaagttcgc tcgccatgat accagccaac ttagcaacct caatcgaatg
    10861 gcgcaaaaca ttttgtccat atgaagtacg gaactgcaaa cgtcccataa tcttcatcaa
    10921 gtctggatga aggtttggcg caccaatttc ataggcagca gcctcaccgt attcacgaat
    10981 cttattgtca atctcttgac ggtttttctc aaccaactct tcgatacgag ctggatgtat
    11041 acgaccatct ttgagcaaca tttccatagt catacgggca atctcacgac gaatcggatc
    11101 aaatcctgac aaggtcacca cttctggtgt atcgtcgata atcacatcga cccctgtcaa
    11161 actttcaaag gtacgaatgt tacgaccttc acgaccaata atgcgtccct tcatagtatc
    11221 gtctggcaga tgaactgttg agtttgttga ctccgctaca tattcaccag cgatacgttg
    11281 catagcttga accaagatgt ccttggccat tttgtcagaa cgttccttga cctcttgctc
    11341 agcttcgcga atgcgactgg caatctccct ggtcaagttt tcctctgtct gagccaagat
    11401 aatatctcgt gcttctgcct gagacagagc accaatacgc tctagttctg cttctttttg
    11461 tctttcgact tcctctaatt gctcttcacg cgcatcaagg tttttcgctc tatcagaaat
    11521 actttgttct ttttgttcaa gtgtttgttc tttactcgtc aaattgtcgt ccttacggtc
    11581 aaggctagta gctctctctg tcaaacgact ttcgatttgt ttgagttctt gacgttctga
    11641 tttgaattca gcgtccactt cttcacggta ttttctggct tcttctttgg cctccaatag
    11701 tgcttctttt ttaagagact tgctttcacg tttggcttca ttaacaagta aatccgcttc
    11761 acgctcagct tgtccacgta aattagttgc ttcttgttca gcatttaaaa gcatcaactc
    11821 tgcagcttcc tgagatgatt tcatcttagc tgagatgctg acatatccaa tgactaaacc
    11881 aatgatgacg gcaaaaacag caatcgcaag cgacatgatt tccatgtttt tacctcattt
    11941 tattgttatt ccgaatgaca tacattcttt tacattctac cataaaaaag tgattttcac
    12001 aaacctaaaa tagaatatgt tttggggaat ttggaacaca tttgcaaaaa taaacttgtt
    12061 gtttagaaat agtagtttag tagagacttg agaaaaagcc tactttacag tagacttagt
    12121 aatgatcttt aaaggacaag aaagccacgc tatctccatc catcatataa atcaagcgat
    12181 tttctgcatc aatacgccgt gaccaggctc cttggtaatc atatttgagt ggctctggtt
    12241 ttcctattcc tgtaaatgga tcgcgttgaa tatccttgat taatttattg attcttttta
    12301 gtgttttttt atcttggttt tgccagtagc aataatctgc ccaggcatct tctgtaaact
    12361 tgagtagcat tcatcactcc tcaatcacat ggacctgagt gcttccagca cggacttgag
    12421 ccattcctcg taaaaccttg tctgataact ccttattttg ggcgattctc agggtttcct
    12481 gaatactgtc ccattcgctc tttgaaagaa ctacaatgtc ctcatctgga tttttattga
    12541 ccaccgtcaa aggctcaaat tcatcattta ccttcttcat gtagtccttt aaatgatttc
    12601 ggaatgttga gtaaaggact gcttccataa ccatacctcg ttttagctct tttccactat
    12661 tatacacgaa aagaaagaaa ttgtcaggaa cttgtacaag attttctttt ctatctattt
    12721 atactcaatg aaaatcaaag agcaaactag gaaactagcc gcaggctgta cttgagtacg
    12781 gcaaagcgac gttgacgcga tttgaatttg attttcgaag agtattattc gtaaaaaatc
    12841 tcaaaaagcc tacctttcag tagacttagt ttgtttctat tctaattggc actctgccaa
    12901 aattttgctc tgctatactt ggctttccta gttggtaaat ctggtcagcc ttttgagtca
    12961 tagcatccca aggttctttg ccaattcggc tgactagact gacctgacct ttcaaagact
    13021 tgagatgttg cctgcctttt tcagtaaatc caaggacatg aatggcttct ggcaagttac
    13081 tttctctagc ctgcaccaag atataggaca agaggcgtct gacacgcgcc ttggtgtaac
    13141 gtttggtagt cactaactcg accaattctt ccacagactg ggctgtttta atagcttcct
    13201 taatgcgcac agccatttct tgattgacct gatagatggt ggttaggtcg ggatttgaca
    13261 agatttgata gcggagcaag ggaaaatagt cttcccagat caccttactg gcctgctcaa
    13321 atagggcaac agaaggcata aaacgttcta agaaatcttg gtccttctga tgttgacgga
    13381 gggctgtcgc cgaggcaaag tccacatctt tattcacaga atggtaacct gccccctgac
    13441 gctgaatcgg atgcagcttg atatttcgtc ctgcaaccgc cttggcatag gccagagcaa
    13501 gaacatgatt gggggtatta cctgaaaaat caagaccagc aaattccttc cacatagctt
    13561 gggttttctg gggataggaa agggaatcag gcagattttc cacaaatttc tccatctcag
    13621 cacctttctc tgtgtataag tcagcgattt tctgataatc tcgaacttcc tctgttccaa
    13681 aaactagact atcaatgccc aagcgatcca agatattcac agctccttga ccaaagaaat
    13741 cagctgcctg aacactgact aaaaagggca attccactac caagtctgcg ccattttcca
    13801 gcgccatctg ggcccttgtc cacttatcca caatagcagg ctctccacgc tgcatgaaat
    13861 tccctgacat ggcaacaatt ttcagtccct ctgcctgatc cagcaggtat ttatggccat
    13921 tatgaaaagg attgaactct gcgataatac ctgtgatggt catgatgttc tcctacttct
    13981 gcgccacaaa aaaccaacgg gtgctagttt ctgttggctc cttatcctca aagtctgcat
    14041 agagtttgaa ggacttaaat ccagcctgtt ccagcaagat atcataggtc aagatctcat
    14101 aggtcctctc ctcatgtact tcatcgtggc gactaaagga accgtcagcc tccttgataa
    14161 agaaagtcag ctcatgcacg atggagtgag gaacttcgtc ctcatatgta tcccaaagca
    14221 tggcaaaatc ttccgcattt tcatggtagg aatagccagg gaatacttca tctgtctggt
    14281 aggtcgagtg cacgtcaaag atgaaaactc cctcttcatt cagcgcattg tacacgtcct
    14341 taaagacgtc ccctacttcc acctcatcct gcatatagca gatagagtcc gaataacacg
    14401 tgacaaaatc gtatttccct gccttggaca aattcagcat attaccttct ataaaagcaa
    14461 tcttttgctt ggctgaagtt gctctcttct ccgcaatctt caacatatcc gcactcaagt
    14521 caagtccagt cacatcaaaa ccagcctgag agaagcgcac tgattgaatt cctgtcccac
    14581 aagccaattc caagagtttc tttctctcct tggtcttagg caaatgacgc agagaaaaat
    14641 tcgtccattt gtcgtataaa ctatcgtcca tgaccgcatc atagaccgcc gcaaaggttt
    14701 cataagtcgc cataatcatg atactaagag ctccatggta aacaccacta gccctttacc
    14761 tttctatcaa tttttctaaa ataagcaaag aaacccagtt gactacaact gagttcatct
    14821 tctaagcaag agcttctgaa atatctactg aattcgcctc atgccatagc ttctctagat
    14881 tataatgggc acgcatttct tctgaaaaga tatgcacgac aacagcacca aggtccagta
    14941 agacccagcc tccagctgca tcgccttcga catggctacc tttaaagcct gcttgagcta
    15001 ctttttcacg gatattagct gcgatagcgt ccaactgacg gctattcatt gagctagtga
    15061 tgacaaagta gtccgtcaca ctagtcaaat cttgtacatc aagtgcgagg atatcctccg
    15121 cacgtttctc atcagccgct ttcacgacta gttctagtaa ttctttttcg ttcatttaat
    15181 cctcttttta gaaaatatgt ttgtaatcta gaacatctgc aaagcgctcc tcccaaatgt
    15241 cctcataaaa ttcattaatc gtttctgctg gaatgtcatc cccatcaaag gttgtgacag
    15301 caccttctgg aacaataagc tgatagccat attcaaaggc aaccttgaca gaggtatcca
    15361 cacaatattc tgtctgcata ccacataaaa ctaatttttc aatcccctgc ttatccaagt
    15421 attcttttaa gccagtttct ttgaaaatac tgttatattt cttctgaaag accttttcag
    15481 caggttttcg atttaaaagc gcagataact gccaatcttc tgatgtttga gcttcagagt
    15541 tctcaatatg ttgaacatag ataatttcaa tattcttgct tctagcctgg ttttgtaaat
    15601 aagaaatttt tcccaataga ctttttgtct ggaatccagt ttctactaaa acattctgaa
    15661 catcaatgat tataaaagcc gtcttcattt agttctcttt caaatagtgc acaaaggcgt
    15721 tataggtttc aagggtttgg ggatagatgg ggaatccctg atgagctaga tactccacgg
    15781 tacgagctgt ttcgtaggcc accgccttat tgagcgatag acttgcaatc tcacgcgcca
    15841 catctactcc aggaaaggct cgattgtgct cgatatagtc tgcgacgtag atgaccttat
    15901 ctaggtctgt catttgacca gcaccgactg tatggacttc aatggctcgc aggatttctg
    15961 aatcatgcaa atccaaatct tcctgaattt tgtaaattcc gaccatacca tgccagacat
    16021 tattgcccca gtttttgagg tcagggtcta gctggtaacg gtcaatcaag tctagaaatt
    16081 cctgatctga caacttttta gcatagtcat gaagaagacc tgctagactt gctttctcaa
    16141 catctacacc aaagcgctga gctaattcca tagcggcacg ctccacacct agacaatggg
    16201 ttagacgttt ctcaggtaga agctctgcca ttttttccaa caaggcctca cgggagcagt
    16261 tgatatagtc ttgataggac atcagtagag cccctccttc tcgatgtagt ctagcactgg
    16321 ctgaggtagg agaaagttgg gtttccgacc ttgggcaagg aaggcacgca ccatgctgga
    16381 cgagatatcc atgagcggta cgtccaccca gataactgga taggaagtcc ctgccttgta
    16441 gcgtggacgc tgaaccccca caaactgaac catgtcaacc agttcatcaa ttcggtacca
    16501 cttaggcaga tagtcaacca tgtcggcacc gatgataaag taataatccg tatctggatt
    16561 cttctctgtc aaaatcttca tggtgtcgta ggtgtaggaa atacccttac gctccaactc
    16621 aatggtttca atgactaggc cgtcaattcc ctcaattgcc aactcaagca tcttgagacg
    16681 atggtgttca gggatggttt cctttttatc aacgtgagga ggttggtatt caggcatgag
    16741 aagaacttga tccagtccca actgttgccg tacttgatcc gcaacaatga gatgggcatt
    16801 gtgaacaggg ttaaaattcc cccctaaaat cccaacttgt ttgcgttttt tctccttgat
    16861 ttctggctcc aactctacct tggtaaaggg agtcaatagt tcgattgtca taggctagtc
    16921 ccctttattt ctctataaaa acagttattt ggagtttcgg tcttagattt ctttgacttt
    16981 cttagaaatc ttgcgatttt ctttcttgct agattgttta aacaaaatca agatgcgtcc
    17041 tattttttgg actgtatcca caccgatttc ttcttccaaa atttcagcta cttcgtggat
    17101 gttttcatct gtgttttgta agagagtaac cttgattaat tcacgcgcat caagagcttg
    17161 acggacgctg gttttgattt ggtcgttgag tccatttttc ccgatttgga tgataggttt
    17221 gagggtgtgt gcctggctgt tgaggaaggc acgttgtttt gatgttaatg acataatttc
    17281 tttaaaagag tttcttttta tacttttctg aagtggtgac ggacgtcagc aaagtccttc
    17341 ggactttcat gactaaaatt tgagcctaag gtctcaaatt ttccgcagtt ggtacaatca
    17401 cttgtaccaa cttacaccac agcgaaaagt attctctatg gggctcgcct tgctcgccca
    17461 aattcagaca accctttcct ttctgtgctt aaataattgc ttttcgtgtg acgacggcga
    17521 cgccttctgg tgcccagacg gcgacttttg ctatgcctgt tacacgaatc catcctaggc
    17581 ctgagatgac taggtctgtc ttatctttaa tggtaaagac atgctggact agccttggga
    17641 aatcttcttt ttccttgcta tttggtggtg tcagaagagt tcccaggtgc ttatcgtaga
    17701 aagcactagc tccttcaagc ttgctacgat ggagtttgag ttcattatca aagaaagcag
    17761 taaatccttg cttttctcct gctataaagt caaagcgtcc caaaccacct aaaaataggg
    17821 tttgctcagg attaagctga taggtcttag gcttgatttc ctttttagga ctgacatact
    17881 tgaggttttt ggccgtcaag tagtgagcca tctggtggcg gtggataatt cccggcgtat
    17941 cgtaaatata agatccgtcg tcaagcggaa tctctatttt gtccaaggtt gtccctggga
    18001 agcgtgaagt agtgatgaca ttctgatcac ccgtgatttc ttggataata gcattgatta
    18061 gagttgattt tccaacgttg gtcacaccga ccacatagac atcgcggccc ttacggtagt
    18121 gttcaatctt gtcaatgact tccttaatgg catgtttatt ttgtgctgaa gttaggacca
    18181 catcgactgg acgaagacct tcttcatggg cacgtttcat gagccactgg ctaatcttac
    18241 cagacttaac tgacttagga aggatatctt ttttatttcc taccaagagg acatcattgc
    18301 ccgagacgaa acgtggtaaa cctgggatga cagatccatt aaaatcaaag atatcaatga
    18361 cattgaccac taaagcatca ctgtctccca cctcgtgcaa gagcttgagg aaatcatcgt
    18421 tcgtcaactg gacatctgtg atttcattgt agtggcggag acggaaacag cgttggcaat
    18481 agacttcgcc agtctccaaa cctttttcaa gtgccgactg gggggtaaaa ccaagaccag
    18541 ctttatctgt cgtctgaatg gttgctccac aaccaataca gagaatttct tccatagtta
    18601 aattcctttt ttatatgtaa tcggtccgta cttttcagtg atttttctca taacacgacg
    18661 ctcacgagtt cggttaatct gcgttttgat tgagtcatgt tggaccaagg gtttgactaa
    18721 aattgaccga atccctgcac ggtgggctgc tcgtatatct gtcatgagct ggtcaccaac
    18781 catgaccact tcctttttgt catagtggaa ttccttcata gcacggtcaa taccaaatgt
    18841 gaagggcttc aaggcccagt aaacgtaatc aatcccaaat ttctcaactg ctcgttgaac
    18901 gcgttttttg gtgttatttg acactacgat aatgccaata cccgcgtccc gaaggtcatg
    18961 tagccattgc ttcatctctg gcgttccatc agggttgttc caagcaatga gggtattatc
    19021 caaatcgacc aaaacagcct tgattccctg cgcctgcagg cttgggactg tcagatcata
    19081 gactgcttcc acagcaaaat ctggtatata attttcaatc gccattctgg ctccttttct
    19141 taactttttt tcgcaatttt ccttagtaac aaggataaaa caatccacaa acctgcacta
    19201 gccaaactga tatagagcca agcatggggc tcatctgtta aaggtaaggg aacattcatt
    19261 ccgaaaaagc ctgtcacgac tgccaaaaca gctagcaaga ctgaaatgat agtcaaggtt
    19321 gtcaaattgt cattcagatt attgtttaga atattgttgt aagaggctga aagctgctgt
    19381 aaaatctgag agattaggtc tgtcatggat accagctgat gagcctcaat catggcatca
    19441 tcaaactgtt ctctctcaat ctcatcaaaa ctacgataca aggcatgacc ttgaatatgc
    19501 tctaacaaaa tccgattttg tttggcagct gccgtcagat aaaccatacc agtctccaaa
    19561 tcagacaggg caaagaggtt tttcttggta gttcgctggc gcaagaggtc attgacctca
    19621 tccctactct tgtccatctg ctcaatgaca ggatagtagg cattgctgat aatttccaga
    19681 ctggcaaaga gaaacttata aatcgaaagc gtgtcatggc tctccagata acgagtcatc
    19741 tgttcaatga cataggcgtt cttggtatta ctaatggtaa tcaggcgacg atgctcgaca
    19801 ataaaggtca tgggaaaagt ctcatagtag gccttgtcct tttttacgtc taggacatta
    19861 tagataaagg taaccgtccc actttcacgg tggtaatcca tgtgggcgcg ctcgtttcta
    19921 tccagtgcgt attcaatggt ttccttgtcc aaaccgtaaa tttcagaaag gtcttctagt
    19981 ttattcaact ttcctaggtc taggtctatc caggtacaac cattgcccaa ctgcttttct
    20041 aaaaccatct tttctcctta ttaaactccc tctattgtac cacaaattac ttaaaataac
    20101 aggtctagtc tgtgcgtgag aaattgctaa caactgtgat atttcggtta gggacaaagt
    20161 gaagggcttg ctcctcgcta cgaagctgtg tcgtacgaat tcccacactg acaaccttac
    20221 ccgatacagt aatcggtcca tttgtcagaa cgacctcatc tcccacatcc agttgacgtt
    20281 caaagaggat gaaaaagcca ttgatgacat cagacagaaa gccttgggct cccataccaa
    20341 tcgctacccc agcaatacca gctccagcca gcaaactaga aactggcaaa cctaaaatcg
    20401 acaaaatgca gtagagtaaa aagaaatata gcgtataatt aaacacattt tctagtaaac
    20461 gtgagatggt tttttggcgt ccgacatcat gacgagacat ttttagagaa ggtttgacaa
    20521 ttctctgcac catggtatga agcatttttt tagcaatata aaatacaatc aaaagcagta
    20581 aaagagaaat gaccttggtt agaatattct cgataatggt tgtcacatct agcttttcaa
    20641 tataagcctg aataaatttt tgcatataaa actcctttat ttcattatac catattttca
    20701 tcagtctcat tctccataaa tattcaaaaa atagacagaa aattcttgat tttgtctatc
    20761 atttatacta taatcataat cactacacaa aaaggagatt attatgaaaa gaatcattca
    20821 tgcttggaat aaggcaagcc ttgtcaagcg tatcttgatt ggtatgcttt gtggggaatc
    20881 ctaggactga cccttcctaa tctctcaggg attgggctac tcggggattt attcgttgga
    20941 ggtctaaaag ctgttgctcc tatcctagtc tttgctctcg ttgccaatgc cctttcccaa
    21001 catcaaaagg gacaagatag caatatgaaa actgttgttt tcttgtatat cctatgatag
    21061 ggactttcgc cgctgctctt gtagctgtac tagcaagttt catcgtccct attgaaatta
    21121 ccctaaatag tgccaatact gaaattgcac caccagatgg gattgggcag gtcctcagca
    21181 acctcttgct caaactggtt gacagcccag tcaacgccct gcttactgct aactatattg
    21241 gaatcttatc ttgggcagtc atttttggaa tcgctatgag agaagccagt aaaaatagta
    21301 aagaattgct aaaaactatc gctgacgtga cttctaaaat tgtcgaatgg atcatcaatc
    21361 tggctccatt tggaatcctt ggtcttgttt ttaaaaccat ttctgacaag ggagtcggaa
    21421 gccttgccaa ctacggtatt ttattggttc tattagtaac gactatgctt tttgttgccc
    21481 ttgtggtcac cctttgattg ccttcttctt tatgagacgc aatccttacc ctctagtttg
    21541 gaactgcctc cgtgtcagcg gtgtgacagc ctttttcact cgtagttctg cggctaacat
    21601 tcctgtcaac atgaaactct gccatgacct tggactcaac ccagatacct attctgtttc
    21661 tatcccactc ggttctacta tcaatatggc tggagtagcg attaccatta accttttgac
    21721 ccttgctgca gttaacactc ttggaattcc tgttgacttt gccacagcct ttgtcctcag
    21781 tgtggtagca gctatctcag cctgtggtgc ttcaggtatt gccggaggtt ccctccttct
    21841 tatcccagtt gcttgtagcc ttttcggtat ttctaacgat attgccatac aagttgtttg
    21901 ggttggtttt gtgattggtg tcatccaaga ctcatgtgaa acagccctta actcttctac
    21961 agaagatgtc ctctttaccg ccgttgccga atacgcagca acccgtaaaa aataactcat
    22021 caaggcaagc ctgcttatgt cttgtctttt acgcttttat tctaacttat taggaaattc
    22081 ttatgtctat tagccaacgt acgaccaagc tcatcttagc tacctgtctt gcctgcctgc
    22141 ttgcttattt tctcaatctt tcatcagcag tttcggctgg aattatcgct ctcttgagcc
    22201 tatctgatac gcgtagaagt actttaaaac tggctcgcaa tcgtcttttt tctatgcttc
    22261 tagctctggc tatcggtgtt ctagcttttc acttgagcgg atttcatatc tggagtctcg
    22321 gcctctatct ggccttctac gttcctttag cctacaagat gggctgggaa attggcatca
    22381 caccaagcac tgttttggtt agccatctct tggttcaaga gtcaacctct ccagaccttc
    22441 tagtcaatga attccttctc tttgctattg gtacaggatt tgccttgctt gttaatctct
    22501 atatgccttc acgagaagag gaaatccagc actaccacac gctggtggaa gaaaagttaa
    22561 aagatatcct ccagcgcttc aaatactatt tatccagagg agacggacgc aaccgagcac
    22621 agctggtagc agaattagac acgcttttga aagaagccct cagactggtc tatttggatc
    22681 actctgacca cctctttcac cagaccgact accatatcca ctactttgag atgagacaac
    22741 gacaaagtcg tatcctgaga aacatggccc aacagattaa cacttgtcac cttgccgcca
    22801 gtgaaagcct gatcttagcg caactctttt caaaaattgc aggtcaactg agccagacca
    22861 atcctgcttc tgatttgcta gatgaaattg aacgttatct ggaagtcttc cggaaccgca
    22921 gtctgcccaa gacaagagaa gagtttgaaa cccgcgccac tcttcttcaa ctcctacgtg
    22981 aagccaaaac cttcatccaa gtaaaagttg acttttatca aaaatataga cagtaatact
    23041 cttcaaacta cgtcagcttc accttgctgt atata
//
LOCUS       DBERMR010000031        23057 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.031, whole genome shotgun sequence.
ACCESSION   DBERMR010000031 DBERMR010000000
VERSION     DBERMR010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 23057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23057
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.031"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            377..2530
                     /gene="comA"
                     /locus_tag="ACL8A1_001346"
     CDS             377..2530
                     /gene="comA"
                     /locus_tag="ACL8A1_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter ComA"
                     /protein_id="HGS1202107.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPYKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTGINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNINLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLIVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2543..3892
                     /gene="comB"
                     /locus_tag="ACL8A1_001347"
     CDS             2543..3892
                     /gene="comB"
                     /locus_tag="ACL8A1_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence pheromone export protein ComB"
                     /protein_id="HGS1202108.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTVKSAIETGASLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4062..4769
                     /gene="purC"
                     /locus_tag="ACL8A1_001348"
     CDS             4062..4769
                     /gene="purC"
                     /locus_tag="ACL8A1_001348"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HGS1202109.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQELK"
     gene            4771..4914
                     /locus_tag="ACL8A1_001349"
     CDS             4771..4914
                     /locus_tag="ACL8A1_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HGS1202110.1"
                     /translation="MFATENLRLSTKRTQAEKVPQTFSLCRELGELTDVYEIVWEKLQ
                     GLK"
     gene            4971..8696
                     /locus_tag="ACL8A1_001350"
     CDS             4971..8696
                     /locus_tag="ACL8A1_001350"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HGS1202111.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLTEDLFAPAEKHIFSEQGTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDAFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTVFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            8789..10231
                     /gene="purF"
                     /locus_tag="ACL8A1_001351"
     CDS             8789..10231
                     /gene="purF"
                     /locus_tag="ACL8A1_001351"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HGS1202112.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     CDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDSLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10268..11290
                     /gene="purM"
                     /locus_tag="ACL8A1_001352"
     CDS             10268..11290
                     /gene="purM"
                     /locus_tag="ACL8A1_001352"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HGS1202113.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKTLEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11287..11832
                     /gene="purN"
                     /locus_tag="ACL8A1_001353"
     CDS             11287..11832
                     /gene="purN"
                     /locus_tag="ACL8A1_001353"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HGS1202114.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKADYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            11916..12425
                     /locus_tag="ACL8A1_001354"
     CDS             11916..12425
                     /locus_tag="ACL8A1_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HGS1202115.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLIFFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVRVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12450..13997
                     /gene="purH"
                     /locus_tag="ACL8A1_001355"
     CDS             12450..13997
                     /gene="purH"
                     /locus_tag="ACL8A1_001355"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HGS1202116.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            14119..15381
                     /gene="purD"
                     /locus_tag="ACL8A1_001356"
     CDS             14119..15381
                     /gene="purD"
                     /locus_tag="ACL8A1_001356"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HGS1202117.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVKKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            15638..15754
                     /locus_tag="ACL8A1_001357"
     CDS             15638..15754
                     /locus_tag="ACL8A1_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HGS1202118.1"
                     /translation="MKERLDDNPIVQGNWKTLGFQFRHETPLVAAAVPHKLR"
     gene            15784..16272
                     /gene="purE"
                     /locus_tag="ACL8A1_001358"
     CDS             15784..16272
                     /gene="purE"
                     /locus_tag="ACL8A1_001358"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HGS1202119.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTILPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16259..17350
                     /gene="purK"
                     /locus_tag="ACL8A1_001359"
     CDS             16259..17350
                     /gene="purK"
                     /locus_tag="ACL8A1_001359"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HGS1202120.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLVDKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            17360..17587
                     /locus_tag="ACL8A1_001360"
                     /pseudo
     CDS             17360..17587
                     /locus_tag="ACL8A1_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
     gene            17650..18948
                     /gene="purB"
                     /locus_tag="ACL8A1_001361"
     CDS             17650..18948
                     /gene="purB"
                     /locus_tag="ACL8A1_001361"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HGS1202121.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(19003..22929)
                     /gene="strH"
                     /locus_tag="ACL8A1_001362"
     CDS             complement(19003..22929)
                     /gene="strH"
                     /locus_tag="ACL8A1_001362"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored beta-N-acetylhexosaminidase StrH"
                     /protein_id="HGS1202122.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKKSARTVDLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVTAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLDDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYILGQKPEDGGG
                     FLKKAIENTGKTPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEILSQPAPAEESK
                     ALPQDPAPVVIEKKLPETGTHDSAGLVVAGLMATLAAYGLTKRKED"
BASE COUNT         6233 a         4631 c         5526 g         6667 t
ORIGIN      
        1 tactaaaatt tttttcttta cttaataatg atatgaaatc atttcggttt tgttaacttg
       61 taattcttat attttctagg tagaacgttt gttctgccta tttttttatc caaaaagtgc
      121 agttgggagg gagataggct catttgggaa ggaagtccag tttttgttta gtgattgggg
      181 taagatagtt gttatcagat gagtttatac tcttcgaaaa tcaaattcaa atcacgtcaa
      241 cgtcgccttg ccgtatatat gtgactgact tcgtcagtcc tatctacaac ctcaaaacag
      301 tgttttgagc agcctgcggc tagtttccta gtttgctctt tgattttcat tgagtattag
      361 ggaaaaggag atgaatatga aatttgggaa acgtcactat cgtccgcaag tggatcagat
      421 ggactgcggt gtagcttcat tagccatggt ttttggctac tatggtagtt attatttttt
      481 ggctcacttg cgagaattgg ctaagacgac catggatggg acgacggctt tgggcttggt
      541 caaggtggca gaggagattg gttttgagac gcgagccatt aaggcggata tgacgctttt
      601 tgacttgccg gatttgactt ttccttttgt tgcccatgtg cttaaggaag ggaaattgct
      661 ccactactat gtggtgactg ggcaggataa ggatagcatt catattgccg atccagatcc
      721 cggggtgaag ttgactaaac tgccacgtga gcgttttgag gaagaatgga caggagtgac
      781 tctttttatg gcacctagtc cagactataa gccttataag gaacaaaaaa atggtctgct
      841 ctcttttatc cctatattag tgaagcagcg tggcttgatt gccaatatcg ttttggcaac
      901 actcttggta accgggatta acattgtggg ttcttattat ctgcagtcta tcattgatac
      961 ctatgtgcca gatcagatgc gttcgacact agggattatt tctattgggc tggtcatcgt
     1021 ctacatcctc cagcaaatct tgtcttacgc tcaggagtat ctcttgcttg ttttggggca
     1081 acgcttgtcg attgacgtga ttttgtccta tatcaagcat gtttttcacc tccctatgtc
     1141 cttctttgcg acacgcagga caggggagat cgtgtctcgt tttacagatg ctaacagtat
     1201 catcgatgcg ctggcttcga ccatcctttc gattttccta gatgtgtcaa cggttgtcat
     1261 tatttccctt gttttatttt cacaaaatat caatctcttt ttcatgactt tattggcgct
     1321 tcctatctac acagtgatta tctttgcctt tatgaagccg tttgaaaaga tgaatcggga
     1381 caccatggaa gccaatgcgg ttctgtcttc ttctatcatt gaggacatca atggtattga
     1441 gactatcaag tccttgacca gtgaaagtca gcgttaccaa aaaattgaca aggaatttgt
     1501 ggattatctg aagaaatcct ttacctatag tcgagcagag agtcagcaaa aggctctgaa
     1561 aaaggttgcc catctcttgc ttaatgtcgg cattctctgg atgggggctg ttctggtcat
     1621 ggatggcaag atgagtttgg ggcagttgat tacctataat accttgctgg tttactttac
     1681 caatcctttg gaaaatatca tcaatctgca aaccaagctt cagacagcgc aggttgccaa
     1741 taaccgtcta aatgaagtgt atctagtagc ttctgagttt gaggagaaga aaacagttga
     1801 ggatttgagc ttgatgaagg gagatatgac cttcaagcag gttcattaca agtatggcta
     1861 tggtcgagac gtcttgtcgg atatcaattt aatcgttccc caagggtcta aggtggcttt
     1921 tgtggggatt tcagggtcag gtaagacgac tttggccaag atgatggtta atttttacga
     1981 cccaagtcaa ggggagatta gtctgggtgg tgtcaatctc aatcagattg ataaaaaagc
     2041 cctgcgccag tacatcaact atctgcctca acagccctat gtctttaacg gaacgatttt
     2101 ggagaatctt cttttgggag ccaaggaggg gacgacacag gaagatatct tacgggcggt
     2161 cgaattggca gagattcgag aggatatcga gcgcatgcca ctgaattacc agacagaatt
     2221 gacttcggat ggggcaggga tttcaggtgg tcaacgtcag agaatcgctt tggcgcgtgc
     2281 tctcttgaca gatgcgccgg tcttgatttt ggatgaggcg actagcagtt tggatatttt
     2341 gacagagaag cggattgtcg ataatctcat ggctttggac aagaccttga ttttcattgc
     2401 tcaccgcttg actattgctg agcggacaga gaaggtagtt gtcttggatc agggcaagat
     2461 tgtcgaagaa ggaaagcatg ctgatttgct tgcacagggt ggcttttacg cccatttggt
     2521 caatagctag aaagaggaga ggatgaaacc agaattttta gaaagtgcgg agttttataa
     2581 tcgtcgttac cataattttt ccagtagtgt gattgtaccc atggcccttc tgctcgtgtt
     2641 tttacttggc tttgcaactg ttgcagaaaa ggagatgagt ttgtccacta gagctactgt
     2701 cgaacctagt cgtatccttg caaatatcca gtcaactagc aacaatcgta ttcttgtcaa
     2761 tcatttggaa gaaaataagc tggttaagaa gggggatctt ttggttcaat accaagaagg
     2821 ggcagagggt gtccaagcgg agtcctatgc cagtcagttg gacatgctaa aggatcaaaa
     2881 aaagcaattg gagtatctgc aaaagagcct gcaagaaggg gagaaccact ttccagagga
     2941 ggataagttt ggctaccaag ccacctttcg cgactacatc agtcaagcag gcagtcttag
     3001 ggctagtaca tcgcaacaaa atgagaccat cgcgtcccag aatgcagcag ctagccaaac
     3061 ccaagccgaa atcggcaacc tcatcagtca aacagaggct aaaattcgcg attaccagac
     3121 agttaagtca gctattgaaa caggtgcttc cttggccggt cagaatctag cctactctct
     3181 ttaccagtcc tataagtctc agggcgagga aaatccccaa actaaggttc aggcagttgc
     3241 acaggttgaa gcacagattt ctcagttaga atctagtctt gctacttacc gtgtccagta
     3301 tgcaggttca ggtacccagc aagcctatgc gtcagggtta agcagtcaat tggaatccct
     3361 taaatcccaa cacttggcaa aggttggtca ggaattgacc cttctagccc agaaaatctt
     3421 ggaggcagag tcaggtaaga aggtacaggg aaatctttta gacaagggga aaattacggc
     3481 aagtgaggat ggggtgcttc atcttaatcc tgagaccagt gattctagca tggttgcaga
     3541 aggtgcccta ctagcccaac tttatccatc tttggaaaga gaagggaaag ccaaactcac
     3601 agcctatcta agttcaaaag atgtagcaag aatcaaggtc ggtgattctg ttcgctatac
     3661 tacgactcat gatgccggga atcaactttt tctagattct actattacaa gtattgatgc
     3721 gacagctact aagactgaga aagggaattt ctttaaaatc gaggcggaga ctaatctaac
     3781 ttcggagcag gctgaaaaac ttaggtacgg ggtggaaggc cgtctacaga tgattacggg
     3841 caagaaaagt tatctacgtt attatttgga tcaatttttg aacaaagagt aatgttcgtg
     3901 tttttagagt taaataattt ttaaactgtg agaaagattc ttcttgcagt tttttcttta
     3961 caatttttga aaaacatctg ctatttattc ggttaaattc ttgtgttttt tggttttttg
     4021 tggtaaaatg tgctcaagta atacgaaagg cgaactttaa aatgtcaaaa caattgatct
     4081 attcgggaaa agctaaagat atctatacaa ctgaggatga aaatcttatt atttcaactt
     4141 acaaggacca ggcgactgct ttcaacggtg tcaagaagga gcagattgca ggtaagggag
     4201 tcttgaataa tcagatctca tcttttattt ttgagaaatt aaatgcggct ggtgtggcga
     4261 ctcactttgt ggagaaactt tcagacacgg aacaactcaa taaaaaggtt aagattattc
     4321 ctttggaagt cgtgctccgc aactatactg ctggttcctt ttcaaaacgt tttggtgtgg
     4381 atgagggaat cgccttggag actccgattg tcgaatttta ctacaaaaat gatgatttgg
     4441 atgatccatt tatcaatgat gagcatgtga aattcctaca gattgcgggt gaccagcaga
     4501 ttgcctactt gaaggaagaa acgcgtcgta tcaatgaact attgaaagtc tggtttgctg
     4561 agattgggct taaattgatt gactttaagc tagagttcgg ttttgacaag gatggcaaga
     4621 ttatcttggc agacgaattt tcaccagata actgccgctt gtgggacgct gatggcaacc
     4681 acatggataa ggatgttttc cgtagaggat tgggagaact aaccgacgtt tatgagattg
     4741 tttgggaaaa gttgcaggaa ttgaaataat ctgtttgcaa cggaaaacct tcgtctctca
     4801 actaaaagga ctcaggctga aaaggtcccc cagacctttt cactctgtag agaactaggt
     4861 gaactaacag atgtttacga aattgtctgg gaaaagttgc agggtttaaa ataacaacct
     4921 caaggctgtt tgggaatatt gcaagagctg aaataaagga ataagaattg atggataaac
     4981 gtatttttgt tgaaaaaaag gctgattttc aggtcaagtc agagagtttg gttagagagc
     5041 tccagcacaa cttgggactg tcaagcttga aaagtattcg tattgtgcaa gtatatgatg
     5101 tatttgactt gactgaggac ttgtttgcac ctgcagagaa gcacattttc tctgagcagg
     5161 gaaccgacca tgttttagat gaagtatctg tgcaggcgga tcttgctaac tatgctttct
     5221 ttgccattga aagtctgcca gggcagtttg accagcgtgc agcttcgtca caggaagcct
     5281 tgcttttgtt gggaagttcg agtgacgtga cagtcaacac agcccaactt tacttggtga
     5341 ataaagatat tgatgcgact gagttggaag ctgtcaaaaa ctacctgctc aatccagttg
     5401 attctcgttt caaggatatc acgacaggga ttgccaagca ggagttttca gagtcagaca
     5461 agaccattcc aaaattgact ttctttgaaa gctatacagc agaagacttt gctcgctaca
     5521 aggccgagca agggatggcc atggaagtgg atgatttgct ctttatccaa gactacttta
     5581 agtcaatcgg gcgcgtgcca actgagactg aactcaaggt tttggacact tactggtctg
     5641 accactgccg tcatacgact tttgagacag agttgaaaca catcgacttt tcagcttcta
     5701 aatttcaaaa gcaattgcag tcaacctatg acaagtatat tgccatgcgc gaggagttag
     5761 gtcggtctga aaaaccacaa accttgatgg atatggcgac tattttcggt cgttatgagc
     5821 gtgctaacgg acgattggat gatatggaag tctctgacga aatcaatgcc tgctcagttg
     5881 aaattgaagt ggatgttgat ggtgtcaagg aaccttggct cctcatgttt aaaaatgaaa
     5941 cccacaacca tccaacagaa attgagccat ttggtggagc ggctacctgt atcggtggag
     6001 ctattcgtga tccgttgtca ggccgttcct atgtttacca agccatgcgt atttcaggtg
     6061 ctggtgatat tacagcaccg atttcggaaa ctcgtgctgg gaaattgcca caacaagtca
     6121 tttctaaaac agcagctcat ggttattctt catatggtaa ccagattggg cttgccacaa
     6181 cctacgttcg tgaatacttc cacccaggct ttgtagctaa acgtatggaa cttggtgccg
     6241 ttgttggagc ggctcccaag ggcaatgttg tccgtgaaaa acctgaagca ggtgatgtga
     6301 tcatccttct cggaggcaaa acaggtcgtg atggtgtcgg tggtgcgacg ggctcttcta
     6361 aggttcaaac agttgagtct gtagagactg ctggtgctga ggttcaaaaa ggaaatgcca
     6421 tcgaagaacg caagattcag cgcctcttcc gtaatggcaa tgtcactcgt ctgatcaaga
     6481 agtccaatga ctttggggca ggcggcgtct gtgtggctat cggtgaattg gcagacggtc
     6541 ttgaaatcga cctcaacaag gtgcctctta aataccaggg cttgaatggt acagaaattg
     6601 ccatctctga atcacaagaa cggatggcgg tcgtggttcg tcctgaagat gtggatgcct
     6661 tcgttgccga atgtaacaaa gaaaatattg atgctgttgt ggtggcgaca gtaactgaaa
     6721 aaccaaatct tgtcatgcac tggaatggtg agacaatcgt tgacttggag cgtcgtttcc
     6781 ttgacaccaa tggtgtgcgc gtggttgtcg atgccaaagt tgtggacaag gatgtcaaac
     6841 tcccagaaga gcgtcaaaca tctgctgaaa cactggaatc agataccctt acggttctat
     6901 ctgacctcaa ccatgcaagt caaaaaggat tacagactgt ctttgactgc tctgttggac
     6961 gctcaacggt taatcaccca cttggtggtc gttaccaact cacaccaact gaggcatctg
     7021 tgcagaaatt gccagttcaa cacggtgtga ctcatactgc gtcggtcatt gctcaaggtt
     7081 tcaacccata tgtagctgaa tggtctccat accacggtgc tgcttacgcg gttatcgaag
     7141 caactgctcg tttggtggct gctggtgcca actggtctaa ggctcgtttc tcttaccaag
     7201 aatatttcga gcggatggac aagcaagcag agcgttttgg tcagccagta gccgcccttc
     7261 taggctctat tgaagctcag attcaacttg gcttgccatc tatcggtggt aaggactcca
     7321 tgtctggtac ctttgaagaa ttgaccgttc cgccaacctt ggttgccttt ggggtgacga
     7381 cggcagatag ccgtaaggtg ctctctccag aatttaaagc tgttggggaa aatatctact
     7441 acatcccagg tcaagccctc tctgcagaga ttgattttga cttgattaag aaaaattttg
     7501 ctcagtttga agcaagccaa gctgaccata aagtgacatc tgcatcagct gtcaaatacg
     7561 gtggtgtagt tgaaagtttg gctcttgcta cctttggaaa ctatattggt gcagaggtga
     7621 ccttgcctga acttaaaaca gctttgacag ctcaattagg cggctttgtc ttcacatctc
     7681 ctgaagaaat tgctggagta gagaagattg gacaaacgaa agcagacttt acactgactg
     7741 tcaacggtgt gaagctagat ggacacaagc ttgacagtgc atttcaaggg acattggaag
     7801 aagtttaccc aacagaattt acccaagcga aagaactaga agaagtacca gctgtggcat
     7861 cagatgttgt gattaaagcc aaagaaaagg ttgaaaaacc tgtggtttac atcccagtct
     7921 ttccaggaac caactcagaa tatgattcag ctaaggcctt cgaaaaagaa ggtgcagagg
     7981 tcaatttggt gccattcgtg accttgaatg aagaagctat tgtcaagtca gttgaaacta
     8041 tggttgacaa tatcgacaag actaatattc tcttctttgc cggtggattc tcggctgcgg
     8101 atgaaccaga tggttcagct aagtttatcg tcaatatcct gcttaatgaa aaagtgcgtg
     8161 tggctattga tagctttatc gcccgtggtg gcttgattat cggtatttgt aatggattcc
     8221 aagccttagt caaatcgggt ctcctaccat acggaaactt tgaagatgct aacagtacta
     8281 gtccaaccct cttctacaat gatgccaacc aacacgtggc caagatggtg gaaactcgca
     8341 ttgccaatac caactcacca tggttggctg gtgtgcaagt gggcgatatc cacgctattc
     8401 ctgtttcgca cggtgaaggg aagtttgtcg tgacggctga ggaatttgca gagctccgtg
     8461 acaatggaca aattttcagc caatacgttg actttaacgg taaaccaagt atggattcta
     8521 agtacaatcc gaatggttct gtccatgcca tcgaaggaat taccagcaag aatggtcaaa
     8581 tcatcggtaa gatgggccac tcagaacgtt atgaggatgg tcttttccaa aatatcccag
     8641 gcaataaaga tcaacacctg ttcgcatcag cggttaaaca tttcactgga aaataagact
     8701 tacagatttt ctaatagata gtatcagtaa tgtaaaagtc atgtaaatct agctcttgat
     8761 gattacaaat gaaaattagg tataaaaaat gacatacgaa gtaaaatctc ttaatgaaga
     8821 atgtggtgtt ttcggtattt ggggacatcc agatgctgct aagttgacct attttggact
     8881 ccacagtctt caacaccgtg gtcaggaggg ggcaggaatc ctctccaacg atcaaggaca
     8941 actgaagcgc catcgtgaca tggggctttt atcagaagtt ttcagaaatc cagctaattt
     9001 ggataaattg acaggagctg gtgcgattgg gcatgtgcgt tatgcgactg ctggcgaagc
     9061 ttctgtagat aacatccagc ccttcctctt ccgttttcac gatatgcagt ttggcttggc
     9121 tcataatgga aatctgacca atgcagcctc tctcaagaaa gaattggaac aaagaggagc
     9181 aattttcagc gcgacttcgg actctgaaat cttggctcac ctcattcgtc gcagtcataa
     9241 tcctagcctg atgggcaaaa tcaaggaagc gctcagcctt gtcaaaggtg gttttgccta
     9301 tatcttgctg tttgaggaca agttgattgc ggctcttgac cctaatggct ttcgtccgct
     9361 ttctatcggg aaaatggcca acggagcggt ggttgtttcc tctgaaacct gtgcttttga
     9421 ggtcattggt gccgagtgga tttgtgattt gaagccaggt gagattgtga tcattgatga
     9481 cgagggcatt cagtatgaca gctatacaga tgatacccag ttggcgattt gttctatgga
     9541 gtatatctac tttgcccgcc ctgattctaa tatccacggt gtcaatgtcc atacggcacg
     9601 taagagaatg ggagcgcaat tggcgcgaga atttaagcat gaggcagata ttgtagttgg
     9661 tgtgcccaat tcttccctaa gcgcggctat gggatttgcg gaagaatcag gcttaccaaa
     9721 tgaaatgggt ctgatcaaaa accaatacac ccagcgaact tttatccaac cgactcaaga
     9781 attgcgggag caaggagtgc ggatgaaact gtctgctgtt tcgggtgttg tcaaaggcaa
     9841 acgtgtggtc atggtggatg attccattgt acgtggaaca acttctcgtc gtatcgttca
     9901 gctcttgaaa gaagcgggtg cgactgaggt tcacgttgcc attggaagtc ctgcactagc
     9961 gtatccatgt ttctacggga ttgatatcca gacccgtcag gagctgattg cagccaatca
    10021 tacggtcgaa gaaactcgcc aaatcattgg tgcggacagt ctgacttatc tttcaattga
    10081 tagcttgatt gagtcgattg gtatcgaaac agatgcgccg aacggtggtc tctgtgtcgc
    10141 ttactttgac ggtgactacc caacgcctct ctatgactac gaagaagact atcgtagaag
    10201 tttggaagaa aagaccagtt tttacaagta ggcgacagat tctccattaa agaaaaggaa
    10261 aaaacaaatg acaaataaaa atgcatatgc ccagtcgggt gtggatgttg aagcgggtta
    10321 tgaagttgtt gaacggatta aaaagcacgt ggcccgtacg gagcgtgcag gtgtcatggg
    10381 agctcttggt ggctttggtg gtatgtttga cctttccaag actggggtta aagaacccgt
    10441 cttgatttca gggactgacg gtgtcggaac caagctcatg ttggctatca agtacgacaa
    10501 gcacgatacc atcgggcagg actgtgtggc catgtgtgtc aacgacatca ttgctgcagg
    10561 tgcggaacct ctctattttc tcgactacgt agcgacaggg aagaatgaac cagctaagct
    10621 agaacaagtg gttgctggtg tggcagaagg ttgtgtgcag gctggtgctg ccctcatcgg
    10681 tggggaaacg gctgaaatgc cgggcatgta cggcgaagac gactatgact tggctggttt
    10741 tgcggtcggt gtggctgaaa aatctcaaat cattgacggt tcaaaggtgg tagagggaga
    10801 tgttcttctc ggacttgctt caagtgggat tcactcaaat ggttactctt tggttcgtcg
    10861 tgtctttgcg gattacacag gtgaggaagt cctaccagaa ttggaaggca agaaacttaa
    10921 ggaagttcta cttgagccga ctcgtatcta tgtcaaggct gtcttgccac tcatcaaaga
    10981 agagttggtc aacggcattg cccacatcac aggtggtggc tttatcgaaa atgtccctcg
    11041 tatgtttgca gatgacctag ctgctgaaat tgatgaaagc aaagttccag tgcttccaat
    11101 tttcaaaacc cttgaaaaat acggtcagat taaacacgaa gaaatgtttg aaatcttcaa
    11161 tatgggtgtg ggacttatgt tggcggtcag ccctgaaaat gtagagcgtg taaaagaatt
    11221 gttggatgaa gcagtctatg aaattggtcg catcgtcaag aaagaaaacg aaagtgtcat
    11281 tatcaaatga aaaaaatagc ggtttttgcc tctggtaatg gctcaaattt tcaggtgatt
    11341 gccgaagaat ttccagtgga gtttgtcttt tcagaccatc gtgatgccta tgtgcttgag
    11401 cgtgcaaagc agctcggcgt tctgtcctat gcttttgaac tcaaggagtt tgagagcaag
    11461 gcagactacg aagcagccct tgtcgaactc ttggaagaac accagattga cttggtttgc
    11521 ctagcaggct acatgaaaat cgttggacca accttattgt cggcttatga aggtcggatt
    11581 gtcaacattc atccagccta cttgccagaa tttccaggag ctcatgggat tgaggatgct
    11641 tggaatgctg gcgtgggtca gtctggtgtg accattcact gggtggattc gggtgtggat
    11701 acaggccagg tcatcaaaca ggttcgtgtg ccacgactag ctgatgatac cattgacaga
    11761 tttgaagctc gcatccatga agcagagtac aggttgtatc cggaagtagt gaaggctcta
    11821 tttacagatt gactttttga tgattcatat gatatctttg attttaaatt ggagtcagtg
    11881 tttgttgaag acggcttcaa acggaggtat ttgtaatgtt agaatctaaa aaaacaactc
    11941 gatatgtatt ttatgtctat ctgatgttat taacttgggg aatcttattt aagtttgaaa
    12001 caaatcctga atttatagca tttttcttag ctccaaggta tatcaattgg attccatttt
    12061 cagaaccact aatagtcgat ggaaaaattg tttttgctga aatgttattt aatctgattt
    12121 tctttattcc attaggtgtt tgtttccctt tgataaaaac taatttatct agtttaagaa
    12181 tagtcgggac aggtttcttg attagtttat tgtttgagtg cttacagtat attttagcaa
    12241 taggtataac agatataacg gatttgactt taaatacgct aggtgtccgt gtaggcttac
    12301 tgatttatca aatttttata agagtgttca aatcacagac tagaaaatgg atcaatatct
    12361 taggtatgct tagccttggt tttgcttatc ttgttttact gttactgcat ttaattggtg
    12421 tttaactaat gattaaaaag gagaatataa tgactaaacg cgtcttaatc agcgtctcag
    12481 acaaagcggg cattgttgaa tttgcccaag aactcaaaaa acttggttgg gagattatct
    12541 caacaggtgg aactaaggtt gcccttgata atgctggggt ggataccatt gctatcgatg
    12601 atgtgactgg tttcccagaa atgatggacg gtcgtgtgaa gaccctccac ccaaatatcc
    12661 acggagggct tctcgctcgt cgtgacttgg atagccactt ggaagcggct aaggacaaca
    12721 agattgagct cattgacctt gtggtggtca acctttaccc atttaaggaa actatcctta
    12781 aaccagatgt gacttatgct gatgcagttg aaaatatcga tattggtggg ccatctatgc
    12841 ttcgttcagc agcgaaaaat catgccagtg ttacagttgt ggtagatcct gctgactacg
    12901 ctgtggtttt ggacgaattg gcagcaaacg gcgaaacctc ttatgaaact cgccaacgtt
    12961 tagcagccaa agtatttcgt cacacagcgg cttatgacgc cttgattgca gaatacttca
    13021 cagctcaagt gggtgaaagc aagcctgaaa aactcacttt gacttatgac ctcaagcaac
    13081 caatgcgtta cggtgagaat cctcaacaag acgcggactt ttaccagaaa gctttgccta
    13141 cagactactc cattgcttca gccaaacagc tcaacgggaa agaattgtca tttaataata
    13201 tccgtgatgc agatgctgct atccgtatca tccgtgactt caaagatagt ccaaccgttg
    13261 tggctctcaa acacatgaat ccatgtggaa ttggtcaagc tgatgacatc gagactgctt
    13321 gggactacgc ttatgagtct gacccagtgt ctatctttgg tgggattgtc gtcctcaacc
    13381 gtgaggtgga tgctgcgaca gctgagaaga tgcacggcgt tttcctcgaa atcatcattg
    13441 caccaagcta tacggatgaa gcgctagcca ttttgatcaa taaaaagaaa aacttgcgta
    13501 tccttgcctt gccatttaat gctcaagagg ctagcgaagt ggaagcagaa tacacaggtg
    13561 tagtcggtgg acttctcgtg caaaatcaag acgtggtcaa ggaaagccca gctgactggc
    13621 aagtggtgac taaacgtcag ccaactgaga cagaagcgac tgctcttgag ttcgcttgga
    13681 aggctatcaa gtacgtcaaa tcaaatggta ttatcgtgac caacgaccac atgacacttg
    13741 gtgttggtcc aggtcaaacc aaccgtgtgg cttctgttcg ccttgccatt gaccaagcca
    13801 aagatcgtct ggacggggcg gtccttgctt cagatgcctt cttcccattt gcggataacg
    13861 tggaagaaat cgccaaagca ggaattaagg ccatcatcca gcccggtggc tctgtccgtg
    13921 accaagaatc tatcgaagcg gcagacaaat atggcttgac catggtcttt acaggtgtga
    13981 gacattttag acattaagaa gataaaaggg aagaaaacag tttctttcct tttttggctt
    14041 aaaatgctaa ctgaaacaag attaaaacga acttttttga tataatgttg gtaaataatt
    14101 cgcaaaagag gttgaggaat gaaactgctt gttgtcggtt ctggtggtcg tgagcatgcg
    14161 attgctaaaa agttacttga atcaaaagac gtgaaaaaag tctttgtagc tcctgggaat
    14221 gatgggatga ctctggatgg tttggaattg gtaaatatct ctatttccga acattctaaa
    14281 ttgattgact tcgcaaagac caatgatgtt gcttggacct ttatcggtcc agatgatgcc
    14341 cttgctgctg gtatcgtgga tgattttaac caagctgggc ttaaggcctt tggtccgact
    14401 agggctgcag cggagctgga gtggtccaag gatttcgcca aggaaatcat ggtcaaatat
    14461 ggtgtttcga cagcagccta tggcacattt tcagatttcg aggaagcaaa ggcctatatc
    14521 gaaaagcatg gtgcgcctat cgtagtcaag gcggatggct tggcgcttgg gaagggtgtc
    14581 gtcgttgctg agacggttga gcaagcggtc gaagccgctc atgagatgct tttggacaat
    14641 aaatttggtg actcaggtgc gcgcgtggtt attgaggaat tccttgaagg agaggaattt
    14701 tcactctttg cctttgtcaa tggtgataag ttctacatca tgccaacggc tcaggaccac
    14761 aaacgtgcct acgatggcga caaagggcct aacacgggtg gtatgggtgc ctatgcgcca
    14821 gttccacact taccacagag tgtagttgat acagcggttg acaccattgt caagccagtt
    14881 ctagaaggga tgattaaaga aggtcgccct tatctgggag ttctttacgc agggcttatc
    14941 ctgacagctg atggaccgaa agtcattgag ttcaacgctc ggttcggaga tccagaaact
    15001 cagattatct tgcctcgctt gacctctgac tttgctcaaa atatcacaga tatcctggat
    15061 agcaaggagc caaatatcat gtggacggac aagggtgtga ctctgggtgt ggttgtcgca
    15121 tccaagggct acccgctaga ctatgaaagg ggcgttgagt tgccagccaa gacagaaggc
    15181 gatgtcatca cctactatgc aggggctaag tttgcggaaa atagcagagc actgctctca
    15241 aacggcggac gagtttatat gctggttacc acagcagata ccgtcaaaga agcccaagcc
    15301 agcatctacc aagaactatc ccaacaaaaa atagaaggac tcttctaccg aacagatatc
    15361 ggaagcaagg caattaagta aagatataag aataacgcgc cgtagtcgcc aaacacgata
    15421 atggtcgtcg tggtgaaaag accagaacag tgaatgttct ggtcaggggg aaacttggag
    15481 accgtaggct caaagtttag gaatgaaacc gaaggtttgc ttccgcctcc atcacctaag
    15541 accattatca aaaagaaaaa taaaaattca caaaatacgt taatgatcgt atggtttgcg
    15601 agcgttaacg agctaatata gaacaatcac cgccgttgtg aaagaacgat tggatgataa
    15661 tccaatcgtt cagggaaatt ggaagacctt gggtttccaa tttaggcatg agacaccttt
    15721 ggtggctgct gccgtccctc acaagctaag gtgattgttg aaaaagagga aaaaggagaa
    15781 gaaatgaaac cagtaatttc catcatcatg ggctcaaaat ccgactgggc aaccatgcaa
    15841 aaaacagcag aagtcctaga ccgcttcggt gtagcctacg aaaagaaagt tgtttccgcc
    15901 caccgtacac cagacctcat gttcaaacat gcagaagaag cccgtagccg tggcatcaag
    15961 atcatcatcg caggtgctgg tggcgcagcc catttgccag gtatggtcgc tgctaaaaca
    16021 atccttccag tcattggtgt acctgtcaag tctcgtgctc ttagtggagt ggattcactc
    16081 tattctatcg ttcagatgcc gggtggggtg cctgttgcga ccatggctat aggtgaagct
    16141 ggagcgacta acgcagctct ctttgccctc cgtctcctct ctgtagaaga taagtccatt
    16201 gcggatgcac ttgccaactt tgctgaagaa caaggaaaaa tcgcagagga gtcgtcaaat
    16261 gagctcatct aaaacaatcg gaattatcgg tggcggtcaa ctgggtcaga tgatggccat
    16321 ttctgctatc tacatgggcc acaaggttat cgcgctggat cctgcggcgg attgcccggc
    16381 ctctcgcgtg gcggaaatca ttgtggcacc ttataacgat gtggacgccc tccgtcagtt
    16441 ggcagaccgt tgcgatgtcc tcacttacga gtttgaaaat gtcgacgctg acggtttgga
    16501 tgccgttatc aaggatggac aactccctca aggaacagat ctgctccgca tttcgcaaaa
    16561 tcgtattttt gaaaaggact ttttgtcaaa caaggctcaa gtcactgtgg caccctacaa
    16621 ggtcgtgact tctagcctag acttggcaga tatcgacttg tcgaaaaact atgtcctcaa
    16681 gactgcgact ggtggctacg atggtcatgg acaaaaggtt attcgttcag aagcagactt
    16741 ggaagcagcc tatgcgctag cagactcagc agactgcgtc ttggaagaat ttgtcaactt
    16801 tgaccttgag atttctgtca tcgtgtcagg aaatggcaag gaggtgacgt ttttcccagt
    16861 tcaggaaaat atccaccgca acaatatcct gtctaagacc atcgtaccag cccgcatttc
    16921 tgaaagtcta gtagacaagg ctaaagctat ggcagtgcga atcgcagaac aactcaactt
    16981 gtctggaact ctctgtgtgg aaatgtttgc gactgatgat gacgttattg tcaatgaaat
    17041 cgccccacga ccacataact ctgggcacta ttctattgaa gcctgtgatt tctctcagtt
    17101 tgacacccat attctcggtg ttctcggagc accattacca gtcatcaaac tccatgcgcc
    17161 agccgttatg cttaatgtcc tcggtcagca tgtcgaggct gctgagcaat atgtgacaga
    17221 aaattcaagc gcccacctcc acatgtatgg taaaatagaa gcgaagcaca accgcaagat
    17281 gggacatgtg accttgttta gtgatgtacc ggatagtgtg gatgagcttg gggaagggat
    17341 tgatttttag gacaagtcta tgatacaaat tatcgttaat acatttattg aaaaggataa
    17401 gactggagca gttgttgaag tgttgtatgc cagtgctgac caagataagg tacaagctaa
    17461 atatgaagaa ctagctgcat aataccccga aaattattta gctatctatg atgtaccgct
    17521 ggatacggat ttgaatacac tagatcatta cccgtctgtg tttattgaaa aagaggagtt
    17581 tgagtagaaa tcttggttta cctagatagc ttattcccaa cagcttaaga agaaaggaaa
    17641 aattaacaca tgatcaaccg ttactctcgc cctgagatgg cgaatatttg gagtgaagaa
    17701 aataaatacc gtgcttggct tgaggtggaa atcctctctg acgaggcatg ggctgagttg
    17761 ggggaaatcc ctaaggaaga tgtggctttg attcgcaaga aggcggactt tgacatcgac
    17821 cgtattttgg aaattgagca ggagacgcgc cacgatgtgg tggctttcac gcgtgcggtt
    17881 tctgagactc ttggtgaaga gcgcaagtgg gttcactatg ggttaacttc taccgacgtg
    17941 gtggatactg cttatggtta cctctacaag caggccaacg acatcatccg tcgtgacctt
    18001 gaaaacttca ctaatatcat cgctgacaag gccaaggagc acaagttcac catcatgatg
    18061 gggcgtactc atggtgtgca cgctgagccg acaacctttg gtcttaaatt ggcaacttgg
    18121 tacagcgaaa tgaaacgcaa tatcgagcgt ttcgagcatg cagctgctgg tgtagaagct
    18181 ggtaagattt ctggtgcggt tgggaacttt gccaatatcc caccatttgt agaggagtat
    18241 gtctgcgata aacttggcat ccgtgcccaa gaaatctcta cacaagtcct tcctcgtgac
    18301 cttcacgctg agtactttgc ggttcttgcc agcattgcga cttcaatcga acgtatggcg
    18361 actgagattc gtggtctaca aaaatctgag caacgcgaag tagaagagtt ctttgctaaa
    18421 gggcaaaaag ggtcttcagc aatgcctcac aaacgcaacc caatcggttc tgaaaatatg
    18481 actggtctgg cgcgtgtcat tcgtggtcac atgattacgg cttatgaaaa cgtcgctctc
    18541 tggcacgaac gcgatatttc ccactcatca gctgagcgta tcatcacacc agatacgacc
    18601 attttgattg actacatgct caaccgtttt ggaaatatcg ttaagaactt gacagtcttc
    18661 ccagaaaata tgatccgaaa catgaactcg acttttggtc ttatctttag ccaacgggct
    18721 atgttgacat tgattgaaaa aggcatgacc cgtgagcaag cctatgactt ggtgcaacca
    18781 aaaacagcct actcttggga caaccaagta gactttaaac cacttcttga ggcagattca
    18841 gaagtaacat cacgtctcac acaagaagaa atcgatgaaa tcttcaaccc agtttattac
    18901 accaaacgag tggatgatat ctttgaacgt cttggactag gtgattaatt aaaaaataaa
    18961 cagcgagctt caatctcgct gtttattttt tatcgaaaag acttagtctt cttttctttt
    19021 agtgagtcca taggctgcta gtgtggccat gagtcctgcg actactagtc ctgcagaatc
    19081 gtgagttcct gtttcaggaa gttttttctc tattaccaca ggagctggat cttgaggaag
    19141 agctttgctt tcctcagcag gagctggttg gcttaggatt tctagttttg gtttttcttc
    19201 agcaatagcg gcttgtccgt tttcatcgcc tacatgtgtt accatagttc cgacttcgac
    19261 tatttgagta acggcttcct gtgctacgac actatttaca agtgttttca cttccttacc
    19321 atcggcagaa gtgctcacag agtagaagtt gctacgatgt ccattgacac ccttagtaat
    19381 gacttgtgtt tttcctttga gtaagagtgg attttcacga gtcactgtgg taaatggaat
    19441 ttcttctttt tggatatcca gtctaggttt tacctcagta gttggtgcaa gaccactttc
    19501 atcacccttg tgagttacag gagcgccaac ttcaaccact tggtttataa cttctttggt
    19561 tacctggcta tcaaggactg tttctgttgt ttttccattt tcagtgagta cagagatgta
    19621 atgagttcgt tcacctttga ctcctgctgt gataatattt tcctgaccag ctgggaggtt
    19681 aggattttct ttcttgataa cttcaaatgg aatttcttca gttcttgtga tgagttctgg
    19741 tctggtttca acattggcag ccacttcatt ttcatctagg cttcctgaat gagttgcagc
    19801 tggtttgagg ccttgaagag cggcttttag gttggctaca agtgtgtcaa gctcagcttg
    19861 tttattacgg ttgaggttgt aatttagagc tgttttagct gcgtcaaggg cctcaagact
    19921 ttctttacta tatccttcta agtttgtagg aattttagct aattcttcgc ggagagcatt
    19981 ataattagca cggaagtagt ctttgttgtg gtctgcaaag gcagtcatga gttcaaagat
    20041 ttcctcttcc ttgtattcag cgcttggtct atctgcccag attgaaagca tacttccgac
    20101 tgttggaaga tctacttcag gatatttggt agaagctagt tgattgaatg gtgtttttcc
    20161 agtattctca atagctttct tgaggaaacc accaccatct tctggttttt gaccaagaat
    20221 gtagtaccag tcaccgttgg tattcaagaa tttatagcct ttgcttgcta ggtattgagg
    20281 tgatgcgagg ttatatcccc accagccttt agaccagtaa gaaatcaaga catctttgtc
    20341 aaactgaaca tcgtccttgt cttcatagta gaagccatcg ttgaaggcca ttggttgaag
    20401 ccctctttct ttggccatag ctgcgagggt gttggcatat tcggcaaatt tgccatagag
    20461 ttgataccac ttgaggtagt accagccttg ggcactagtc gcatcgttgg cgtattcgtc
    20521 agtaccaaag ttgaaaatct ttgttttacc tgcaaagaag tccatgtatt taccgatgag
    20581 ggcttttaca aagttcatcg cttcttcgtt tttcaagtcc atagttgttt ttgaaacttt
    20641 atcaaagtgg gcttgaggat ttttaatacc taatttttcc atggcaacca gcatagcatc
    20701 catgtgacct ggactgttaa tagctgggat gagaccgatg tccttagatt tagcgtattc
    20761 aattagctct gttacttctg cctgtgttag tgcagtaccg tttggatcgt cgtagtaagc
    20821 tttagttcct tcgataatag cttttttaac gtcatcacta gcataggttt ttccgttggc
    20881 agtaatggtc atatcatcga gtagaaagcg aagtccgtca tttcctagaa ggagatggac
    20941 atcagaatat ccgagctcac tggccttgtc tacgatgcgt ttgagctggt tcagagtaaa
    21001 gtatttgcgt ccagcatcga ttgagattac cttgtttttg gcaagttttt caacctcacg
    21061 tttagcttct tcttcttttt gagcttcagg cgtgagggtc aagttgttga cagtttcttg
    21121 aagtttagca atggcttgat caatcgtatc ttgttgggca cggctaaggt tgctatcgag
    21181 agagcgaata gctttttcag cttcttttac ggccgtgacg ctttctgcag tataacggtt
    21241 caggtctttt ggtacctcgt taagtgcttg ctctgcagat tcataatcag ctgcgaagta
    21301 ttcagcgttg gcatttgcaa aatgacgcat gagtttgaag aggcgtgatg gtgaataacg
    21361 tgcagatgga gtgtcagccc aagcagctac cataccaccg atgattggga tatcagctcc
    21421 ttctgttttt ggtacagaag tgattggtgt gtttttaata ccattgagcc cctgatcgag
    21481 attgtaccag ccttggccat cagcgtttcg tccaagaacg tagtaccaag catcattggt
    21541 attaaggatt tggtgacctt tttcagctag tagtttagaa gaagcgacat cgtagcctcc
    21601 ccaaccacca gtccacatag aaacgatgat gtctttgtca aaactaccaa agcttgtgtc
    21661 gctattgtag tagataccgt cgttaaaggc cattggtttg agaccgtgcg attttacaat
    21721 acgagcgagg tcattggcgt aggcaataaa tttttcatag ccttttacag ggtagccttc
    21781 gtttggatag tatttatcag cttgaagcac actccaacct ttagcatctg tcgcatcatt
    21841 ggcatattca tcaagtccga tgttgaagat ttcagtcttt ttcgcgaaat aagcagcata
    21901 cttgtcgata agggcttttg taaaagcgac agcttgttcg ttgtcaagat cgacagtacg
    21961 ggctgatttc ttcccaaaat agctaaagtt agggttttgg attcccaatt ctttcatggc
    22021 attgagaatc gcatccatgt gtccaggact atttactgtc ggaatgagac cgataccttt
    22081 atctttggca tagttaatca gatctgtcat ttgactttct gttaagtgat tgccgtttgg
    22141 atcgttgtaa taatcatttg tacctttttc aatggcgcgt ttgacatcgt cactggcata
    22201 ggtcttgccg ttagctgtga tgctcatatc gtccaacatg aaacggagtc catcatttcc
    22261 gactaatagg tgtaaatcag tgtagccata atgtttcgct ttatcgatga tttccttgag
    22321 ctgttctggt gagaaatatt tacgtccagc atcaatagaa acaattttct ttttcgctag
    22381 tttttcattt acagttgcag cacgttcctt tcctgcctct gtttccggtt tgtcagcctc
    22441 tgctttcgct tcatcttttt tagctggttt atccttgtca gtcttgtctg tatttgactc
    22501 tttagaatca acctctttcg cttcttcctt tttagggcta gcttcttctg cctttttatt
    22561 agcagtttct ttttcagcag aagttggagt taccacttct gctttatcac taggagttga
    22621 actaacttcc tcttgtggtt tttcttctgt ttttggaaga ctagctacct tatcagtagc
    22681 tggagtttct gtttctacag tttttggagc ttctggttga agctctgctt taggtgtttc
    22741 ctcagtccga ttttcggatg attgagggga atcagaaacc gtatggatag tcggttggtt
    22801 ttctgtagta gtaggagtaa ctccatcggc tgcaacagtc tgtgcttgga aggcaaatcc
    22861 aattagaaca gaagctgctc ctacagcgta tttacgaata gaaaaacgct gttgtttttc
    22921 atgtttcatt gcaaaacctc ctgattgcat tgttatattg atagcgatta tataaatcaa
    22981 cgcctttatt ttatttctta tattaacgag agtcaagagg agatgacaaa aaactataat
    23041 aagtataaaa aaatata
//
LOCUS       DBERMR010000032        22344 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.032, whole genome shotgun sequence.
ACCESSION   DBERMR010000032 DBERMR010000000
VERSION     DBERMR010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 22344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22344
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.032"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            237..1100
                     /locus_tag="ACL8A1_001363"
     CDS             237..1100
                     /locus_tag="ACL8A1_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS1202123.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYLRQHGAGELSKHAEQVVKKLWKKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICTDNAKLIQKGFSLLELTEETSMLSHLKK
                     EVETHYQPKKL"
     gene            complement(1153..1740)
                     /locus_tag="ACL8A1_001364"
     CDS             complement(1153..1740)
                     /locus_tag="ACL8A1_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HGS1202124.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            1982..2653
                     /gene="sdaAB"
                     /locus_tag="ACL8A1_001365"
     CDS             1982..2653
                     /gene="sdaAB"
                     /locus_tag="ACL8A1_001365"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HGS1202125.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            2662..3534
                     /gene="sdaAA"
                     /locus_tag="ACL8A1_001366"
     CDS             2662..3534
                     /gene="sdaAA"
                     /locus_tag="ACL8A1_001366"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HGS1202126.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            3556..4188
                     /locus_tag="ACL8A1_001367"
     CDS             3556..4188
                     /locus_tag="ACL8A1_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGS1202127.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAMQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            4238..4450
                     /locus_tag="ACL8A1_001368"
     CDS             4238..4450
                     /locus_tag="ACL8A1_001368"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202128.1"
                     /translation="MKIKEQTRKLAAGCSKHCFKVVDGTDEVSSKHCFEVADRTDEVS
                     SKHCFEVVDRTDEVSNHIRQGDVDVV"
     gene            complement(4535..6385)
                     /locus_tag="ACL8A1_001369"
     CDS             complement(4535..6385)
                     /locus_tag="ACL8A1_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HGS1202129.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVARAVDEAKVPKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(6424..7584)
                     /locus_tag="ACL8A1_001370"
     CDS             complement(6424..7584)
                     /locus_tag="ACL8A1_001370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGS1202130.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTESFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGFGTIVGLIRGFKWKKEYKGTIIYLDKISQINQNMLQ"
     gene            7642..8808
                     /locus_tag="ACL8A1_001371"
     CDS             7642..8808
                     /locus_tag="ACL8A1_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HGS1202131.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTRLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPSVAALLLIDILMTVALFTVDRADKIL"
     gene            8947..9273
                     /locus_tag="ACL8A1_001372"
     CDS             8947..9273
                     /locus_tag="ACL8A1_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HGS1202132.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWALYTDTINGIIEGS
                     IRHDKN"
     gene            9260..9853
                     /locus_tag="ACL8A1_001373"
     CDS             9260..9853
                     /locus_tag="ACL8A1_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HGS1202133.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLFNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTILAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            9846..10757
                     /locus_tag="ACL8A1_001374"
     CDS             9846..10757
                     /locus_tag="ACL8A1_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HGS1202134.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHALTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILQLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            10820..11884
                     /locus_tag="ACL8A1_001375"
     CDS             10820..11884
                     /locus_tag="ACL8A1_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6574 domain-containing protein"
                     /protein_id="HGS1202135.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADLDTPIEK
                     NTQLEEEVSQAEVELESQQEEKIEAPEDSEARTEIEEKKASNSTEEESDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIVSILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPNQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(11956..12816)
                     /locus_tag="ACL8A1_001376"
     CDS             complement(11956..12816)
                     /locus_tag="ACL8A1_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 family protein"
                     /protein_id="HGS1202136.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIYLVQITGDGSKPEDYESIARLDYAKFLEVLPPSFYHQLDANQIEIQPILG
                     QDFKTLAQEK"
     gene            complement(12938..13924)
                     /locus_tag="ACL8A1_001377"
     CDS             complement(12938..13924)
                     /locus_tag="ACL8A1_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HGS1202137.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEDLWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(14196..15671)
                     /locus_tag="ACL8A1_001378"
     CDS             complement(14196..15671)
                     /locus_tag="ACL8A1_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS1202138.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(15929..16852)
                     /locus_tag="ACL8A1_001379"
     CDS             complement(15929..16852)
                     /locus_tag="ACL8A1_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS1202139.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(16866..17795)
                     /locus_tag="ACL8A1_001380"
     CDS             complement(16866..17795)
                     /locus_tag="ACL8A1_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1202140.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(18114..18335)
                     /locus_tag="ACL8A1_001381"
     CDS             complement(18114..18335)
                     /locus_tag="ACL8A1_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202141.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSQEELQKAVLF
                     LADEKGSEHTAAELIDNLKEVIAKLKANA"
     gene            complement(18289..18549)
                     /locus_tag="ACL8A1_001382"
     CDS             complement(18289..18549)
                     /locus_tag="ACL8A1_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202142.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKALTDDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
     gene            18629..18766
                     /locus_tag="ACL8A1_001383"
                     /pseudo
     CDS             18629..18766
                     /locus_tag="ACL8A1_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(18870..19025)
                     /locus_tag="ACL8A1_001384"
                     /pseudo
     CDS             complement(18870..19025)
                     /locus_tag="ACL8A1_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 transposase-related protein"
     gene            complement(19211..19822)
                     /gene="rpsD"
                     /locus_tag="ACL8A1_001385"
     CDS             complement(19211..19822)
                     /gene="rpsD"
                     /locus_tag="ACL8A1_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HGS1202143.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(20017..21069)
                     /locus_tag="ACL8A1_001386"
     CDS             complement(20017..21069)
                     /locus_tag="ACL8A1_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGS1202144.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVTKLEKGQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(21066..21764)
                     /locus_tag="ACL8A1_001387"
     CDS             complement(21066..21764)
                     /locus_tag="ACL8A1_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGS1202145.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(21931..>22344)
                     /locus_tag="ACL8A1_001388"
     CDS             complement(21931..>22344)
                     /locus_tag="ACL8A1_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat-containing
                     protein"
                     /protein_id="HGS1202146.1"
                     /translation="ATVNVYGNKDGKPDLDNIVATKKVTIKINVKETSDTANGSLSPS
                     NSGSGVTPMNHNHATGTTDSMPADTMTSSTNTMAGENMAASANKMSDTMMSEDKAMLP
                     NTGEAQTSMASIGFLGLALAGLLGGLGLKNKKEEN"
BASE COUNT         6632 a         4796 c         4176 g         6740 t
ORIGIN      
        1 agactaatca ataatataat acatactaga atacaaaata tttttttatt catacttttt
       61 ctcctatctt tattttctac ctacatagca cctcttatta agctcatttt aaaattacgt
      121 taactttttt cacaaattta tttcgacttt cttttttact ttttacattg ctatgataca
      181 atatttttaa caaaatttta ttgtttgtta acttttttca cgaaaaggag aaaatcatgc
      241 gttatgattt cggaaaggtc tataaagaaa tacgtgagtc aaaggggctg acccaagaag
      301 aggtctgtgg gggtgttctc tcaagaacca gcttatcaaa aatcgaaagt ggcaagacaa
      361 ccccaaaata tgaaaatatg gaatttcttc tccgtcaaat caatatgagt tttgaagagt
      421 ttgaatatat ctgtcaactt tatcaaccaa gtcaacgcac agagattatg caaacctatc
      481 tcaatatgcg ttccattatc gggaccagcg atcttgtcaa tttatttcaa aaatgccaag
      541 actacctcaa gacccaccac gatctcccta tagaagaaat cagagatatg cttgaagttg
      601 ttatttatct ccgtcaacac ggggcagggg aactatcaaa acacgcagaa caggttgtca
      661 aaaaactttg gaaaaaaatt gaaaaacaag atacatggta tgagagtgat ctaaaaatcc
      721 tcaataccat tcttttcagt tttcccattg aatacctcca tctcatcact ggaaaaatct
      781 tgcaacgctt agaagtctat aaaaactatc aacatttata tgacttgcga atagcaatcc
      841 tgctaaacct ttccacactc tatctataca atcaagataa aaacatgtgt aagcaaatct
      901 gctacacttt actggaggac gccaagaata agaaaagcta cgataggctt gctatctgct
      961 atgtccgtat tgggatttgt acagataatg ctaaacttat ccaaaaaggt ttctcacttc
     1021 tggagctgac cgaggaaact tctatgctgt ctcatctcaa aaaagaagta gagacccatt
     1081 atcaaccaaa gaaattataa aaaaagtcga gggagctcct cgacctttta catattattt
     1141 gttcgttagt tcttaatacc agccattgtt aagccagaag tttttagcag cagtccatga
     1201 accgtaacgt cctgcaacgt aggcatctgc tacacgttct tggttttcag ctgagtagtc
     1261 accgttcagg tatgaatctg ttaattggta acgtccgata taacgtccat ttgtagctgt
     1321 atagctacca cctgattctt tttgagcgat ccattctttg gcttctgctt cagatccgct
     1381 tacagttgaa acaactgttt cacttactac tggagtttct gccactggag ctgaaacagt
     1441 ttcatcttga gcggctggtg ccgcataagt agctggcgct ggtgttgcaa caggcgctac
     1501 agggccatcg ataaccaact cttgatcaac ataaatcaaa tgaatgttat caatgtggtt
     1561 gttttctgcc aatttttcaa ctgttgtgtt gtgagtttca gcgatttctg aaagtgtatc
     1621 accttcttta acagtgtaag ttgatgattc ttgagcagat acaaatgatg gagcaaatac
     1681 tgcaaacaag gcagctactc ctgcaagagt tgctttaatc tttttagtta ttgatttcat
     1741 attcgaaaat tctccttctt tctatagttc tatcataccc tttaaatatt accgtttctt
     1801 gacactttta tgtagaaata ttacaaaatt attttttatt tccctaaata aggtgttttt
     1861 tgtcataaaa gatactattt ttatgctatt tgaggtatta gaaaggtttt catccacaat
     1921 taaagccaag cccctcattc tttgatataa tagagataag aatttttata aggggaaact
     1981 gatgaaatca cttcgttttc aatctgtctt tgatatcatc ggaccagtta tgattggccc
     2041 atctagtagc cataccgctg gtgctgttcg tattgggaag attgtctctt ccatttttga
     2101 tgatactccg acagaagttg aattccaact ttttaactca tttgccaaga cctatcgtgg
     2161 tcacggaaca gacctagccc ttgttgcagg tattttagga atggatacag atgatcctga
     2221 aattccaaat agtctggaaa ttgcccacaa gcgtggtatc aagattgtct ggaccattca
     2281 gaaagacagc aatgcccctc accctaacac cactaaaatt actgttaaaa atgcccacaa
     2341 gaccatcagc gtgactggta tttctatcgg tggaggaaat attcaggtaa ccgaactcaa
     2401 tggttttgcc gtctctctca atatgaatac accgactatc atcatcgttc atcaagatat
     2461 tccaggtatg attgcccttg ttacagaggc gctttcccgc tatggtatca atatcgccca
     2521 gatgaatgtc actcgtgaaa aagctggtga aaaagccatt atgattatcg aagttgacag
     2581 tcgcaactgt gatgaggcta tcgaagaaat tcgaaaaatc cctcatctcc acaatgtcaa
     2641 tttctttaaa taggaggaag catgttttat tctatcaaag aattggtcga gcaagcagat
     2701 ctagactttc aaggaaatgt cgcagaactc atgattacaa cagagtttga attgaccggt
     2761 cgcgaacgtg aagaagtctt ccttctcatg gaacgcaatc tggaagttat gaaagcctct
     2821 gtccaacttg gcctcaatga aaataaatct cgtagtggcc tgacaggtgg agatgctgcc
     2881 aaattggatc actacatcga aaacggaaaa actctgtcag attatacgat tctctcggct
     2941 gcccgaaatg ccatcgcagt caatgaacac aatgccaaaa tgggcttggt ctgtgccact
     3001 ccaaccgctg gaagtgctgg ctgtctgcca tccgttctca ctgctgctat tgaaaaatta
     3061 gacctcagcc acgagcaaca attggatttc ctctttgctg ctggtgcctt tggactagtc
     3121 atcgcaaaca atgcctccat ctcaggtgct gagggtgggt gtcaagctga agttggttca
     3181 gcctctgcta tgagtgctgc cgccttgact ctggctgcag gtggaacacc ttatcaggcc
     3241 agtcaagcta ttgcctttgt cattaaaaat atgctaggcc tcatctgtga ccctgttgca
     3301 ggtttggtcg aagttccctg tgtcaaacgt aatgccatgg gagctagctt tgctttcatc
     3361 gcagcagaca tggccttggc aggtatcgaa tctaaaatcc ctgtggatga agtgatcgat
     3421 gccatgtacc aagtaggagc aagcatgcca actgcctttc gtgaaacagc tgaaggtgga
     3481 ctcgctacca cccctactgg tcgtcgcctc caaaaagaga ttttcggaga ataagcttat
     3541 caaataggag aaaccatgac ctctatcaca gcgatttttt tcgatttgga tggaaccctc
     3601 gttgatagtt ctatcgggat tcacaatgcc tttacctata cctttaaaga gctgggggtg
     3661 cctagccctg atgccaaaac tattcgtggt tttatgggac cacctctcga aagtagtttt
     3721 gcgacctgcc tgtccaaaga ccaaatttct gaagccatgc agatatatcg ttcttactat
     3781 aaggcaaaag gcatctatga agctcaactc tttcctcaga ttatagactt gcttgaggag
     3841 ttatcgagca gttatccact ctacatcacc acgacaaaga atacatctac tgctcaagac
     3901 atggccaaaa acttggaaat ccatcatttc tttgatggta tttatggttc tagccctgaa
     3961 gcaccccata aggcagatgt cattcaccaa gccttacaga cacatcaact agcaccagaa
     4021 caagccatca tcatcggaga taccaagttt gatatgctgg gagctcgaga aacaggtatt
     4081 cagaaattgg ccgtcacttg gggatttgga gagcaagcag atctactaaa ctatcaacct
     4141 gattatatcg ctcacaaacc cttagaagtt ttggcgtatt ttcaatagca tagactgggc
     4201 gacaactcta tttcagaaga aaatgaagta atactcaatg aaaatcaaag agcaaactag
     4261 gaaactagct gcgggctgct caaaacactg ttttaaggtt gtagatggaa ctgacgaagt
     4321 cagctcaaaa cactgttttg aggttgcaga tagaactgac gaagtcagct caaaacactg
     4381 ttttgaggtt gtggatagaa ctgacgaagt cagtaaccat atacggcaag gcgacgttga
     4441 cgtggtttga agagattttc gaagagtata agtctctaca taataaaacg catattacca
     4501 aggttgttaa cttgataata tgcgtttttc ttttttattc aatgtgggtt gtttgattag
     4561 caaaggcgat aatagcttgc ttcaactcat ctccactgag agtgcggaac tctccaatct
     4621 tttgattgat ggattctaaa ggcatgacat taaccttacc aacgaaaatc ttatccataa
     4681 cttgattgtc aacgagttcg gttgatacca agagttcttc gtagagtttt tcacctgggc
     4741 gtattccaac ttcaacgatt ggaatttcac tttcagtgtg gccacttaga agcaccatct
     4801 tcttggccaa gtcataaatc ttgactggtt tgcccatatc aaggataaag acttccccat
     4861 ctttggcata agcaccagca tggataacca gacggctagc ttctggaatg gtcataaagt
     4921 aacgggtcat gcggaagtct gtcaccgtta caggcccacc ttcagcaatc tgacgttcaa
     4981 agactggaat gacactacca cggctaccaa gaacattccc aaaacgaact gcacagtagg
     5041 ttgattggct acgttggtta aagccagtga caatcaactc cgccacgcgc ttggttgctc
     5101 ccataacatt tggtggatta actgccttat ctgtcgaaat cataaccatc ttaggcacct
     5161 tggcttcatc aacggctcta gcaacattgt aagttccacg gatattgttt ttgaaggctt
     5221 cttttggatt gcgctccatc ataggaacat gcttgtgggc tgccgcatga taaacaatag
     5281 caggtttgta ctgctcaaag acttgcaaca aacgatcata gtcttgaatg tccgcaatca
     5341 caggtacata atcaatccct tggaacttac gaatcaattc atgataaaca aggtagattg
     5401 agttttcccc atgaccgagc aagacaatgc gttcaggatt gaagcgacta acttgacgac
     5461 agatttcaga accgattgaa cctccagctc ctgtgactaa gatggtctta cctgtcagtt
     5521 ctgcacccag acgcgattcg tcaagacgga tttcctgacg acccaaaagg tccgtaatat
     5581 caattttttg gaagccagta cctgcttggt gaaggccctg aacaacagtt tcaaccttag
     5641 gcatcttgta acatttgaca cccagcttat tacacatctg caagatacgc tcatattctg
     5701 acggatccag cgacggaatc gcaacgatga cacgctcgat ttgatggcgt ttggctaatt
     5761 caggcagatt gtcataagag cccaaaacag gaataccacc aagtttttga ccctttttct
     5821 tagaatcctt atccaaaata ccgaccagtt ctaattcact ggttggatgt tggtaactat
     5881 ccataaagag agccccacca tcaccggcac caatcaagaa ggtccgacgg tgttctccat
     5941 caccactacc ttttttacgt ctggagtaga ttaactgcca agtaatccgt ggcaataaaa
     6001 tcaagaaggt actcaacaag ataaagagaa tgatgaaacg gatggagaag agtggcaaga
     6061 aggcataaca gatactatat gacaagacac tgctagcagt cacaccaaaa aagattttca
     6121 tgaaatccgt aatcttgctg taacgactaa tgctcgcgtt caacccccaa aaaccaatca
     6181 tcaattgata gaacaagaag gccaaactcg tatagataat gtagtcaaca ggtgctggat
     6241 taatcagccc ataaaataaa atataagata caatgatgga aaccaccata ctgaaaatat
     6301 caaatattcc ccagaaaacc tgcttttgtt gtttatttaa aatttccacc agatcaatca
     6361 cataatctgt tagtttttta ttcatagaaa tctactcctc cttaaaagag ttttactata
     6421 ttgttattgt agcatatttt gatttatttg gcttatttta tccaaataaa ttattgttcc
     6481 cttgtactcc ttcttccatt taaatcctct aattaaacct acaatcgtcc caaagccata
     6541 agcaaagtga atggaaaata aaagaaaggg catcacaatt agaaatccat ttttatgttt
     6601 taataaagtc agcaaagtga gtagtgacaa aagtagaaaa taggcaccta atagtaaagt
     6661 tatgaatacg aatgtgatcg gtaacaatgc tagactaaac acaagactca aaacaaataa
     6721 acaaggaaca tagtgaaata atgataaaca cttaaactga acatgacttg tcaagccaat
     6781 ccacaaacca tttgaatact tttgatgcag cattttcttg aatgttggtc gaatatactg
     6841 ataagataga atacttgggc tatagcggat tttataacca tgttctcgaa ttctataatg
     6901 aatatcatta tcttcagttc ggccaagttg ctcatttact aaaccaacct tctggaaaac
     6961 ctctcgttta tacatcccat gaaaaataga agaaacatat ctatcctcgg aactatttcg
     7021 ataattggca atgctactgc caaacatatt ttcctcaaca agatgcaagg tctctgccca
     7081 ttttcctttt ccttcgacaa tcgtcggtct aggcccccca cagacaaatt caccttgttg
     7141 aataatagcc acattgttca ttacaaaact ctcagtaact tttgaatgag catcaatttt
     7201 taaaataagg tcccctacag aatgtttaac tcccaggtta aaaccactag cttgattttt
     7261 cttaggattg ttatacaatc taattgagtt aaactctgta tcttccttta taaattgctg
     7321 aatgatagct gtggtcccat ctgtggacat agcatttata aatagaattt caatattctc
     7381 tttaggatag gtttgatttt ttaagtcttc aattagacca ggcagatatt tttcttcatt
     7441 gtatgcactg attacaatgg atacgagaag actttcaaca ttttgagtca ctactactct
     7501 cctgaatttt tccttccacc attctatcat aattttcaag actttcccat ttttatcttg
     7561 aaagccagaa accttacttg acattatagt gaaaaagcct taaaataagc tgttattaaa
     7621 atcagctaga agaggtatta tatgaaaaag caatcactct tttttgttcc aggtattatc
     7681 ctgattggtg tttccttgcg aactcctttt actgttttac ccattatttt gggaaatatt
     7741 tcgcaaggtc tggaggtaga agttagttcg cttggtgtct tgaccagcct gcctctcctt
     7801 atgtttaccc tcttttcacc attttctacc cgactggctc agaaaatcgg cttggagcat
     7861 ctcttcacct acagcctctt cttcttgacc atcggctcac ttattcgact aatcaatctg
     7921 cccctgctct atctaggaac cttgatggtt ggggcaagtg tcgcagtcat caatgtcctg
     7981 cttcctagtc ttatccaagc caatcaacca aagaaaattg gttttctgac caccttatat
     8041 gtaacgtcta tggggattgc aacggctctg gcttcctatc tagctgtgcc cattacacaa
     8101 gccagttctt ggaaaggact tatccttctc ctcacgttac tctgtctagc aacttttttg
     8161 gtctggctcc caaatcaccg ctataatcat agactagctc cacaaaccaa acaaaaaagt
     8221 caaataaagg tcatgcgtaa taaacaggtt tgggcaatta ttatcttttc aggttttcaa
     8281 tccttgatct tttacaccgt catgacctgg ttacctacca tgtctatcca tgcaggtcta
     8341 tccagtcacg aagctggctt gctgacttct atcttatctc tgattagcat tcctttttca
     8401 atgaccatcc caagcctgac aaccagttta tctactcgca accgtcagct catgctcact
     8461 ctggtttcac tagctggtgt ggtcggcatt tccatgctct ttttcccaat caataatttc
     8521 atttactggc ttgccatcca tctcctcatc ggaaccgcaa ccagcgccct cttcccttat
     8581 ctcatggtca acttttcact caagacaagc gcccctgaaa agacagccca attgtccggc
     8641 ctatctcaaa caggaggcta tatcctagca gcctttgggc caaccctctt tggttacagt
     8701 tttgacctgt tccactcttg ggtaccatct gtagctgccc tcttgctcat cgatatcctg
     8761 atgactgtgg ccctctttac agtggacaga gcggataaga tcctttaaac ttctagtttt
     8821 agctagaagt tttttatata taaaaatttt acacatttct cttgacaggg ctttctttta
     8881 gatgtacaat gtatgtgtag aaaaattata tataaaaatc ttacacatta gaaaaggagg
     8941 tttcccatgt actttccaac atcctctgcc ttgattgaat ttctcatctt ggccgtactg
     9001 gagcagggtg attcttatgg ttatgagatt agccaaacca ttaagctaat cgctaatatc
     9061 aaagaatcca cactctatcc cattctcaaa aaattggaag gcaatagctt tctgacaacc
     9121 tattctagag agttccaagg tcgcatgcgc aaatactact ccttgacaaa cggtggtata
     9181 gagcagctct tgaccctaaa agatgaatgg gcactctata cagacaccat caatggcatc
     9241 atagaaggga gtatccgcca tgacaagaac tgaatacctg actcagctag aactctatct
     9301 caagaaacta cctgaagctg accgtatcga agccatggac tatttcagag agctctttga
     9361 cgatgctgga gtcgaaggag aagaagaact catcgctagt ttgggaactc ccaaagaagc
     9421 ggcccacgaa gttctattca atcttctcga taaaaaaatc aatgaagcac ccgctcaaaa
     9481 aaataaccga caaattttac atatcgcctt gttagccctc cttgcagcac ctatcggcat
     9541 tcctctggga atcgccatcc tcgtgaccct gttcgcaatc cttgtagccg ctttgactgt
     9601 cattctggct ttctttgcag tttccatact gggtatcatc ggcggattcc tatttttagt
     9661 tgaaagtttc actatcctcg cccaagccaa atcagccttt atcttgattt ttggttctgg
     9721 tttactggct atcggtgctt cttcgctagt tttacttggc atttcctatg tagctcgctt
     9781 cttcggtcta ctcattgttc gtctggtaca atttgttctt aaaaaaggaa agagaggtaa
     9841 tcagcatgcg taaatggaca aaaggatttc tcatctttgg tgtggtgact accgttatcg
     9901 gctttatcct gctttttgta ggtatccaat ctgacgggat taagagccta ctttccatgt
     9961 ccaaagaacc tgtctatgat agccgtacgg aaaagctaac ctttggcaag gaagtcgaaa
    10021 acctagaaat tactctccac caacacgcgc tcaccatcac agactctttc gatgatcaaa
    10081 tccacatttc ttaccatcca tctctttctg ctcaccatga tcttatcacc aatcagaacg
    10141 atagaactct gagtctcact gataagaaac tgtctgaaac tccgtttctc tcttctggaa
    10201 ttggtgggat tcttcatatc gcaagtagct actctagtcg ttttgaagaa gttattctcc
    10261 agctaccaaa agggagaact ctaaaaggga tcaacatctc agccaatcgc ggacaaacca
    10321 ccatcataaa tgctagcctt gaaaatgcga ccctcaatac aaacagctat atcctccgaa
    10381 ttgaaggaag tcgtatcaaa aacagtaaac tcacaacgcc caatatcgtt aatatctttg
    10441 atacagttct tacagatagt cagctagagt caacagagaa tcacttccac gctgaaaata
    10501 tccaagtcca tggcaaggtt gaactgactg ccaaagatta tctcagaatc atcctagacc
    10561 agaaagaaag ccaacgaatt aactgggaca tctcaagcaa ctatggttct atcttccaat
    10621 tcacaagaga aaagcctgaa tcaagaggta cggaattaag caacccttac aaaactgaaa
    10681 aaaccgatgt caaggatcaa ctcattgcga gatctgatga taatattgat ctaatatcca
    10741 caccaagcag acgttgacaa aaacgcttac atctgctact atggaagcat atttatctaa
    10801 ctaaaaagga ggttctacca tgaaacaaga atggtttgaa agtaatgatt ttgtaaaaac
    10861 aacaagcaag aacaagcctg aagagcaagc tcaagaggtt gcagacaagg ctgaagaaac
    10921 gatagccgat ctcgatacac caattgaaaa aaatactcag ttagaggagg aagtctctca
    10981 agctgaagtc gaattggaaa gccagcaaga agagaaaatt gaagctcctg aagacagtga
    11041 agcgagaaca gaaatagaag aaaagaaggc atctaattct actgaagaag agtcagacct
    11101 ttctaaagaa acagaaaaag tcactatagc tgaagagagc caagaagctc ttcctcagca
    11161 aaaagcaacc acgaaagagc cacttcttat cagtaaatct ttagaaagtc cttatatccc
    11221 cgaccaagct ccaaaatcta gggataaatg gaaagagcaa gtgcttgatt tttggtcttg
    11281 gctagtggaa gcgatcaaat ctcctacaag taagttggaa acaagtatca cacacagtta
    11341 cacagccttt ctcttgctca ttctgttttc tgcatcttcc tttttcttta gtatctatca
    11401 catcaaacat gcttactatg gacatatagc aagcattaac agtcgcttcc ctgagcagct
    11461 agctccttta actctttttt ctatcgtctc tatcctagta gcgacaacac tcttcttctt
    11521 ttcattcctc ttgggtagtt tcgttgtgag acgatttatc caccaggaaa aggactggac
    11581 gctagacaag gttctccaac aatatagtca actcttggca attccaatct tcctcactgc
    11641 tattgctagt ttctttgctt tctttgatag cctacgattt acagccctct tgtgtgtgat
    11701 tagcattgga atcattctgc ttgccagtct ccatatcatt acaagaccaa atcaagcaag
    11761 tgaaaccgac tccttctatc aattattctt gtctgtcctt gtgaacggag ttattatcct
    11821 cctcttcttt gtagctgaag tggcactgat tggagattat cttcgtatct tggcctttct
    11881 ttaaaattca atcctgtcat ccttggcagg attttttcta tgtcaaaaaa gcaagtcgat
    11941 tgacctgctt ctgctttact tttcttgtgc taatgtttta aaatcttgtc ctaggatggg
    12001 ttgtatttct atttgattgg cgtctagttg gtggtaaaaa gatgggggta atacctctaa
    12061 gaattttgca tagtccaagc gagctatgga ttcatagtct tctggtttag agccgtctcc
    12121 tgtgatttga accaagtaaa tctcccactg gacttcctta tccaccaatt gctcaatata
    12181 ggctgcagga acaaacggtt ctctcggtaa aactgtcttg actccttgag ctaaatggta
    12241 aacgccatgc acttttcctt ctccatcctg atagaaggaa actcttgcaa agtaggcatc
    12301 tgtctcttga accgcacgca cacgcgcttc aaactgagcc aagccatctg cctctaaaaa
    12361 gcttttcaag ttctcacgac taaagaaaat aggattaaaa attccttggc aaatcgtaaa
    12421 acgagctaca gggtctgcta gataggcttg aatacttgct tggaaataag cttcaaaatc
    12481 aaaaccatct agcccttcaa tatcagtccc tgtataagca agcaaagcat ctaaaaatga
    12541 cttgataatc tgccagtctg tctttgttag taggtcagga agatcgacac ggtaagcctt
    12601 ttgaccatca gcataactga ccttaaagag aaattgtgat tgccccacta tcgcacactc
    12661 aatatactcg agacgattaa gaggctgacg aagatagact acatcatagc tatgtgactc
    12721 caaaccgtct agcaaggcca agatcgactt ggcattcaaa atctcttgtt ctcctaaaat
    12781 gctctgttta tttggaataa aaaatgtttt cgccataact ggaccccttt gaagtcgttt
    12841 acatacagta taccttattt tcctgaaaga tacaaaaaca aactttagat ttttgagtac
    12901 aaagcataga aaaacagccc ctgaggactg tttgatctta tacagtagct gcctgatcca
    12961 agctttcacc gatagcggct aggcgctcga tcacttcagc ttgtgtcaat tcattttttg
    13021 aaacatagcg gttacgtggg tgaacacggc actcgtgtga gcatccacga aggtacttgt
    13081 cttcattttc ttctgatgtc aagatacgac ggttacagaa gggatttcca cagttgacat
    13141 aacgttcaca tggcgttcca tcaaaccagt ctttccctac gatagttggg ttgacatggt
    13201 tgacatcaac ggcaatacgc tcgtcaaaga cgtacatttt cccatcccaa agctcacctt
    13261 gaacttctgg gtctttaccg taagttgcga ttcctccgtg caattggcca acatctttgt
    13321 aaccttcacg aaccatccag cctgagaatt tctcacagcg aacaccacct gtacagtaaa
    13381 ccacgacacg cttgtccatg aatttttctt tgttgtcacg gacccattgt ggtaactcac
    13441 ggaagttgcg aatatctggg cgaatagccc cacggaaatg tcctaggtcg tactcataat
    13501 cgttacgtgt gtcaaggaca acggtatctt tatcaagaag cgcttctttg aactcttttg
    13561 gagacaagta agcacctgtt gtttcaagtg ggttgatgtc attgtcaaag tcgttgtctt
    13621 ccaaaccaag gtggacaatt tctttcttgt agcgaacaaa catcttcttg aaggcttgtt
    13681 cattttcttc gtcaatcttg aaccagagat cttccattcc tggaaggctg tgaacgtagt
    13741 ccatgtattt ttgagttgtt tcatagtcac ctgaaactgt tccgttaatt ccctcgtcag
    13801 cgactaggat acggccttta aggccgattg atttacagaa agccaagtgg tctgcagcaa
    13861 attgctctgc attttcaatt ggagtataaa ggtagtaaag taagacacga atatcttttg
    13921 ccataagatt tgttctcttt tctattctta aattttctaa attttttatt tatccatact
    13981 agtatacctg cttgagaaaa cgaatgcaat ttatttgact ggaaattgta gagggttctc
    14041 gaaatttcct gaatggttaa aataaggaca agagaaaaca tggatatcta tatccttgtg
    14101 ccaaaaaaac cactgccctc cccagaccaa cctgaggaaa gcagtgattc ttattttagg
    14161 agttaggaat gaatacacga aatcaattta gctgattatt ttttgttttt caagaattca
    14221 tcgtattgtt tttgcatttc gttcaatact ttttcgtagg caccttcaga tttcaatttt
    14281 tccatcaatt ctggaatcgc tttatctggg tctacagtac cagtgttgat agctgtatca
    14341 aattgttgca ttgtgttagc aatagctgag atttcagatt tcacattgtc agtattgaag
    14401 ataaatccaa gcgctggaga ttctttagct tctgccaatt ctttcttaga attttcgatt
    14461 tgttggtctg taacgttttc gttgatgtaa aggatccagt tgttaccagt gttccatcca
    14521 cccatgtgag tgtttccttt gtagccatca agaacgcgaa cacggttttc tttaccttca
    14581 attttttccc agttcttgcc ttctggaccg taaacaagac cgttcaagag ttctggattc
    14641 gtattcaaga ggttcaagat ttccattgat ttttctttgt tcttagagtt gtttgagatg
    14701 acaaagttag caacttgtgt tgtttggttt ttcttgatga agttagtaat tggtttgatt
    14761 tggatatctt tgttggcaac acgtgaaagc aagctgttac cgtagtcagc tggtcctact
    14821 gtttcttcac gaacgaacca agtatcttgt tgaaggtcaa aggaagtatc gcttgttgcg
    14881 acgtcttttg gaatgtagcc agcttcatag aatttgtgaa gagtcttcaa gtgttctttg
    14941 aaacgaggca cttcgtaacg gtttacaact ttagtagtat cgccttcaag gtcgataacg
    15001 aatggaagac cgtttgctac tgggtagtca aagttatcag atgggatgaa aactttacca
    15061 atagcaaatg gtactacgtc tggagctttt tctttaattt gtttcaagac tggctcaaga
    15121 gtttcgtaag aagtaacacc tgaaatatcg ataccatatt tagcaaggag agttccgttg
    15181 aaggcaaagt tttgagatga tgcaacgttg gctgcaactg gaacagcgta aatcttacca
    15241 tttacagtat tacccttgat gtaagctggg tcaagtgctt tgtaaaggtc tttaccttct
    15301 tttttgtaca attctgtcaa gtcagcgtaa gcaccttttt gagcatttac aatatagtta
    15361 tctgcaaagg caatatcata gttttcacca gatgatgtga taactgacat tttcttacca
    15421 tagtcacccc agccaaggta ttggatatcc aatttggcac caactttttc ttcaatgatt
    15481 ttgttggcat ttgctaacaa ttcatccaag ttgtctggtt tgtcaccgat ttggtacatt
    15541 ttgataacag gtttgtcacc tgaatcagca gcttttttgc tgttacctgt caaatttcca
    15601 caagcagcaa gacctgcagc caaagcgact acactagcag atgcaaaagc atattttttc
    15661 cagtttttca tgataaaaac tccttttttt atttttaaac ttataaacaa tgtaatgatc
    15721 ttatactcaa tgaaaatcaa agagcaaact agaaaactag ccgcaggctg ctcaaagcac
    15781 tgctttgagg ttgtagataa gactgacgaa gtcagttaca tatatctacg gcaaggcgac
    15841 gttgacgcgg tttgaatttg attttcgaag agtattaact tcacacaagg gaagttggga
    15901 actgagaaat gttctttttc aataagcact attctttcac accaccgata gtcaaacctt
    15961 ttacaaagta gcgttggaag aatggataca aaatcgcgat tggaagggtt gctaccacaa
    16021 ccatggccat acgacctgtt tctttcggta gagcaactcc cagttgacca gtcaagccga
    16081 ccgctttggc aatgtagtcc atattttgtt ggatttgcat gagtaaatat tgcaatggat
    16141 acaagttgtc actcttgatg taaagaaggg cgttgaacca gtcattccag aaaccaagag
    16201 ctgtcaaaag cgtgatggtt gcgatacctg gtagtgacaa tggcaaacag atttggaaga
    16261 aaatccgggc ctcactggca ccatcgatac gagccgattc gagaatggct tctggaatgg
    16321 tcttcttgaa aaaagaacgc atcaagatga tattgaatgg tgagagaagc attggaacaa
    16381 tcaaggccca aacagtgtca ccaagctgaa gtacacgggt caccatgata taacctggta
    16441 ccaaaccagc gttgaacaac atactgagaa ggacgaagat ggtaaaaaat ctgcgatact
    16501 taaaggttgt ccgtgaaata gcgtaggcat aggttgttgt gataaagaca tttgtcaatg
    16561 tcccaactac ggttacaaag acagagatga agagggcttg taggatttta tccttaaact
    16621 gtgccaaaaa ctcaaaaccg tctaagccaa attgggatgg gaagaagcta tagccgtatt
    16681 ggaggaggct tttctcgtct gtcactgaaa taatgataac gaatacaaat ggtaggatac
    16741 aagagagggc aatcaaaccc gaaatgatac tgaagaagat atctgctttc ttactgaagg
    16801 agtgaatgcc gacattatca attttttctt ttttaatttt cttttttgcc atattctcct
    16861 cctttctaga acaaagctga gtttggatcg actcgtcttg caagcaagtt tgataggata
    16921 accagaatca aaccaacaac ggattggtaa agaccggctg ctgcagccat accgatatct
    16981 gctgtctgag tcaaaccatt aaagacatat acgtccaaaa cgttggttac attgtaaagc
    17041 tgaccagcat tgtgtgggat ttgatagaag agaccaaagt ctgcgcggaa gatatttccg
    17101 actgcaagga tggtcaatac agttacaagc ggagtcaact gaggaatggt tacgttgcga
    17161 atacgttgcc acttgctagc tccgtccact gtcgctgctt cgtagtaggt tggatcaatt
    17221 cccatgatcg tcgcatagta catgacactg ctatatccaa agcctttcca aatacctagg
    17281 aaaagtagga gatagggcca gatgcccagg tcagcgtaaa aattgacttc tttgagacca
    17341 agactttcca atagatgatt gaacacccct ttatcaatat ttaggaaggc atctgtaaag
    17401 aaactgatga taacccaaga caagaagtaa gggaacaaca tagaagtttg gaaaatcttc
    17461 accattctct tagaacggag ctcgctgagg ataatggcaa tccctacaga tacaactaaa
    17521 cctagaaaga taaagccaag attgtagagg acagtatttc gtgtgataat aaaggcgtct
    17581 cttgaactaa ataagaatct gaaattatcg agtccgaccc atttactatt tatgatacta
    17641 tctatgaaac cattactggt catgtggtag tctttgaagg caaccacgtt cccaaatact
    17701 ggaatgtaaa agaatagaat caaccagagt gcccctggca aaaccatcaa gagaaagatc
    17761 cagttgtctc tcaatgtttt tgaaaacttt ttcataattt cctccctttt tattttgata
    17821 tccatctaaa aattcttttt tagacttttg ataacgatta cattattagt atactcctat
    17881 ttgcaggtta ggttaaactc ctaattatag aaaaaactcc acaaattatg tagcagattt
    17941 aaaactttat caccactatc aaacaaatgt cctaaatcaa ttgtttattt tatctctatt
    18001 agcccagtga tggcgtcact ctgttataag catccaacaa cggggtatac tgaaaaatct
    18061 ccagactagg gaactcagcg atagttccta atctggagat ttttaatatg ttattaggcg
    18121 tttgctttca acttagcaat aacctcttta agattatcaa tcaactctgc tgcagtatgc
    18181 tcagagcctt tttcatctgc caagaacaaa actgcttttt gaagttcttc ttgagagttt
    18241 tcaaggacat ccttatctac tgtttcaagg tttgagtctt taagaagttt acttaattcc
    18301 ttggctaatt tcttgagttt gatttgcaga ctcatcttct cctgctgttt ctttgcccgc
    18361 tgtttgtcct ccatccttag ttgctgactg gctttcctta atggactcta gggaagcaat
    18421 ggcatctttg actgtttgca agatatcacg taaaccttgc tctgtcaaac tatcatctgt
    18481 aagagctttc ttagcctctg ccaaaaccag acgtgctgaa tctgtggtag gattcgatac
    18541 acctgtcaat gatctcaaaa gattttctaa agtttgagtc tgcttactaa tactagacta
    18601 aaatcaaaaa gcattatata acagtgatat gaaatcaact aaagaagaaa tccaaactat
    18661 caaaacactt ttaaaagact ctcgtacagc taaatatcat aaacgccttc aaatcgttct
    18721 atttcgtctg atgggcaaat cttataaaga gattatagaa cttttatagt agtttgaaat
    18781 aagatgtgaa caactctatc aggaaagtca aattaattta tagaaatatt ttagcagcta
    18841 aggtgtactg ttatagattc aatacactat acttggtggt ttagctctcc tgttttctct
    18901 tttagcttta accagccata aatggtatta cgtgagattt ggaaaacgtg tgatgcttct
    18961 gttatactac ctgttcgctc acaataagag agaacttttt tacgaaaatc tattgaatat
    19021 gccataaaag gattatacca cattgtgtac tatttttggt tcattttact atatcacttt
    19081 ggactatatc ggtctagtta tgtctaaacg attatagctg gatactaaga gaaatcaaaa
    19141 aagcactaaa ggagaacgtc ccaaagtgct taatatcaag gcctcaaagc ctgtcttatt
    19201 caataaaata ttacaacatc ttgttgtaga attcaacgac aagtgcttcg ttgatttctg
    19261 ggttgatttc gtcgcgttct ggcaagcgag tcaatgaacc ttccaatttt tcagcgtcga
    19321 atgatacgaa tgctggacgt ccaagagtag cttctactgc ttcaaggatt gctggaactt
    19381 tcaatgattt ttcacgaact gagatcactt gacctggagt tacgcggtat gatgggatat
    19441 caacgcgttt cccgtcaaca aggatgtgac cgtggtttac gaattgacga gcttgacgac
    19501 gagtagtcgc gagaccaaga cggtaaacaa cattatccaa acgacgttcc aaaagaagca
    19561 taaagttgaa acctaggatt ccacctttga tttttgtagc ttgtacgaac aagttacgga
    19621 attgtttttc acctacaccg taagtgaaac gaagtttttg tttttcagcc aattgcaaac
    19681 catattctga caatttagaa cggttgtttg gtccgtgttg tcctggtacg tagttacgac
    19741 gtgccaattc tttacctgta cctgtaagtg aaaggccaag gcgacgagct tgtttccaag
    19801 atggtcctgt ataacgtgac atatgaatgt cctcctgata taaataatat ttcggtggaa
    19861 atagtcactt agaaagccct gattcgtgca gatgcccttc gcctaaacag ccaaggttac
    19921 ttgtcataag acacctgttg acgagcttca tgctttcctg ctgatatttc acacaaaatc
    19981 cattttacca tgaatagctg gatttgtaaa ggggttttag gctttatttt cactaccaga
    20041 gagattgaga acgagggtaa aggtacttcc tagaccgtac tggctgctga ctgtgatttc
    20101 cccacccaat tgatgggcca attcacgcgc aatcgcaagt cctaatccat gaccacctgt
    20161 cttcatgtta cgcgaagttt cgacacgata aaggcgtttg aaaatatttt ccaaatcctc
    20221 tggggcaatg ccctgccctt catcggtcac actgattgaa agctggccct tctccagctt
    20281 agtcaccact tccagcttgg ttcctggagc agaatattta aaagcgttat cgaccagatt
    20341 caccaagata cgagaaagct tagcataatc tccctcaatc cgggcagact ctgggattac
    20401 ttgcaagtgg acatctcttc tctcctgctc aatcaaaaac tgaaattcac tcatgcactc
    20461 aattaagagc ttgtccagaa aaatactgtc tttactggta gtttccacct gatttctagc
    20521 tgtatttagg gtcaaaaaat tcaactcctc aaccagttta ttgagcctct ccgtctggcg
    20581 tccaatggtt gctagataat gagcttgctc cgactccttg ataatcccat ccaaaatccc
    20641 ttctaccgtc gcttggatcg aagtgatagg agtcttaata tcatgcgaca actgggcaat
    20701 catcaagccc ttttctcgtt cgctttcttc caaggaatca aagcttacct gcaaatcatg
    20761 ggacatctca ttaaaagttt gccctaattg ctgaaattct acaggacctt gaacctccaa
    20821 atttgaagga aaatccttgg ccgctacccg cttggcatgc tccttgagtt tgcccaacga
    20881 cgtaaagact ggcaatagga gaaagagact aatcccagca ccgacaaggc tggcaacgat
    20941 ggtcatccca agcaaaaagt aaatctcgcc tttcgcaatc agcatttttt gaacagccca
    21001 aaaaacaacc aaaatggtta agagggttga aataatatat ccaaccaaaa tataactttt
    21061 tagtttcatg tttgtcctct cggtttctct atcttatatc ccaaccccca aactgtctta
    21121 atagtgggcg tttggtcact actatatttt gccagctcct gtcgaagagc atggatatgc
    21181 acattcaagg tattggtgtc atccacgtag tcttctttcc agatcttttc atagaggtct
    21241 gtcttggaga aaactcgctc tggattgcta gctaaaatcc acagcaattc aaatgattta
    21301 acagttaaat ccagcatttc ttctcctatt tgaacttcat gactactatg attcattttt
    21361 agattgccaa gggaaatcag ctctgtttcg cccccacgat gaaggcggcg caaaatattg
    21421 tggacacgca aaaccagctc acgtgggcta aaaggcttag caataaaatc atctgccccc
    21481 aagctcaggc cgtaaatctt gtcctgttca ctggtcttag cagtaataaa taggaaaggc
    21541 tgctctggtg ataagtattg aacctcactg attaaatcat aaccatccat ccgaggcatc
    21601 atgacatctg tgataatcaa atcaatcggt tttttcttga acagctctag cgcttccagt
    21661 ccatcatggg ctaccaagac ctgataacct gcctgaatta agtatctctg atgaatatct
    21721 gtgatttcta cctcgtcgtc aacgagtaaa attgtctttc ccatctgtct ctcctttgat
    21781 aaaaacagtg ctatactgct ttaagtataa cactattttt attgttttac gatgatttta
    21841 atctgctatt gaaagaaagt cctgattgct gattgagttg ttaaactaat cttagtcagc
    21901 ccactatcca tcatttattt ccttagctga ttagttttct tcttttttgt ttttcaaacc
    21961 tagaccaccg agtaaacctg caagcgcaag tccaaggaaa ccaatacttg ccattgatgt
    22021 ttgagcctca ccagtatttg gtagcatagc tttatcctct gacatcatcg tatcagacat
    22081 cttgttagca gaagcagcca tattttcacc tgccatcgtg ttggtagaac ttgtcatggt
    22141 gtcagcaggc atgctatctg tagtacctgt agcatgattg tgattcatcg gagtcacgcc
    22201 agaaccagag ttagaaggtg ataatgaacc atttgctgtg tctgatgttt ctttaacatt
    22261 tatcttaata gtgacttttt tagttgctac gatgttgtcc aagtctggtt taccgtcttt
    22321 gttaccatag acattgactg tagc
//
LOCUS       DBERMR010000033        22271 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.033, whole genome shotgun sequence.
ACCESSION   DBERMR010000033 DBERMR010000000
VERSION     DBERMR010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 22271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22271
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.033"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(515..1363)
                     /locus_tag="ACL8A1_001389"
     CDS             complement(515..1363)
                     /locus_tag="ACL8A1_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative RNA methyltransferase"
                     /protein_id="HGS1202147.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            1515..1793
                     /locus_tag="ACL8A1_001390"
     CDS             1515..1793
                     /locus_tag="ACL8A1_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6110 family protein"
                     /protein_id="HGS1202148.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            1795..3858
                     /locus_tag="ACL8A1_001391"
     CDS             1795..3858
                     /locus_tag="ACL8A1_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HGS1202149.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRGFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWLSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(3936..5192)
                     /gene="tyrS"
                     /locus_tag="ACL8A1_001392"
     CDS             complement(3936..5192)
                     /gene="tyrS"
                     /locus_tag="ACL8A1_001392"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HGS1202150.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEESYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADEHNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            5334..7799
                     /gene="pbp1b"
                     /locus_tag="ACL8A1_001393"
     CDS             5334..7799
                     /gene="pbp1b"
                     /locus_tag="ACL8A1_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP1B"
                     /protein_id="HGS1202151.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGVGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            7864..8775
                     /locus_tag="ACL8A1_001394"
     CDS             7864..8775
                     /locus_tag="ACL8A1_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGS1202152.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVSVISASEPLSSALLSVLFLGLVV
                     DWSFLLAIALIILPMIFLSIEEAKESR"
     gene            9005..9703
                     /gene="dapD"
                     /locus_tag="ACL8A1_001395"
     CDS             9005..9703
                     /gene="dapD"
                     /locus_tag="ACL8A1_001395"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HGS1202153.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNVVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            9771..10901
                     /locus_tag="ACL8A1_001396"
     CDS             9771..10901
                     /locus_tag="ACL8A1_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HGS1202154.1"
                     /translation="MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEAGAFGDWLPDQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            10913..11452
                     /locus_tag="ACL8A1_001397"
     CDS             10913..11452
                     /locus_tag="ACL8A1_001397"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HGS1202155.1"
                     /translation="MKSELRKQVLQGMKAISQEQKQFIDQTLTERLLHHPFYQEAKVI
                     ATYLSFPHEFQTQELIEQALKDGKKVLIPKTYPKGRMDFVVYDPQQLVKTSFGLLEPQ
                     GDLEVVDASQIDLIHVPGLAFTTKGYRIGYGGGYYDRYLEHFSGHTLSTVHPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            11436..12113
                     /locus_tag="ACL8A1_001398"
     CDS             11436..12113
                     /locus_tag="ACL8A1_001398"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HGS1202156.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIILRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(12180..13079)
                     /gene="galU"
                     /locus_tag="ACL8A1_001399"
     CDS             complement(12180..13079)
                     /gene="galU"
                     /locus_tag="ACL8A1_001399"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HGS1202157.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDETTGMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FEILEKQAPGAGNEIQLTDAIDTLNKTQRVFAREFTGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(13101..14117)
                     /locus_tag="ACL8A1_001400"
     CDS             complement(13101..14117)
                     /locus_tag="ACL8A1_001400"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HGS1202158.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKNVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            14365..14469
                     /locus_tag="ACL8A1_001401"
                     /pseudo
     CDS             14365..14469
                     /locus_tag="ACL8A1_001401"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            14634..14768
                     /locus_tag="ACL8A1_001402"
     CDS             14634..14768
                     /locus_tag="ACL8A1_001402"
                     /inference="COORDINATES: protein motif:HMM:NF037495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202159.1"
                     /translation="MFEEYYDRLPEEERFIIPNYSYLALTNYTVQKLPEKLVEILSFW
                     "
     gene            complement(14973..15314)
                     /locus_tag="ACL8A1_001403"
                     /pseudo
     CDS             complement(14973..15314)
                     /locus_tag="ACL8A1_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(15376..16026)
                     /gene="phoU"
                     /locus_tag="ACL8A1_001404"
     CDS             complement(15376..16026)
                     /gene="phoU"
                     /locus_tag="ACL8A1_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HGS1202160.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELACARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(16041..16793)
                     /gene="pstB"
                     /locus_tag="ACL8A1_001405"
     CDS             complement(16041..16793)
                     /gene="pstB"
                     /locus_tag="ACL8A1_001405"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HGS1202161.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(16795..17610)
                     /gene="pstA"
                     /locus_tag="ACL8A1_001406"
     CDS             complement(16795..17610)
                     /gene="pstA"
                     /locus_tag="ACL8A1_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HGS1202162.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(17603..18466)
                     /gene="pstC"
                     /locus_tag="ACL8A1_001407"
     CDS             complement(17603..18466)
                     /gene="pstC"
                     /locus_tag="ACL8A1_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HGS1202163.1"
                     /translation="MTKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFIANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(18584..19459)
                     /locus_tag="ACL8A1_001408"
     CDS             complement(18584..19459)
                     /locus_tag="ACL8A1_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HGS1202164.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(19626..20957)
                     /gene="pnpS"
                     /locus_tag="ACL8A1_001409"
     CDS             complement(19626..20957)
                     /gene="pnpS"
                     /locus_tag="ACL8A1_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system histidine kinase PnpS"
                     /protein_id="HGS1202165.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(20958..21665)
                     /locus_tag="ACL8A1_001410"
     CDS             complement(20958..21665)
                     /locus_tag="ACL8A1_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGS1202166.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(21749..22235)
                     /locus_tag="ACL8A1_001411"
                     /pseudo
     CDS             complement(21749..22235)
                     /locus_tag="ACL8A1_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855144.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
BASE COUNT         6445 a         4236 c         4824 g         6766 t
ORIGIN      
        1 ggacggattt attttggatt ctttttgtaa ttggggcatg cctcttgacg ggtaatagta
       61 gtcttttccc ccttacccat caagaattct cttttcgtgg tagcgtttgg ggtctggtac
      121 tggccttata tcacttattg ttcattgata agtttgtaat ttcgaatcga aaataaagat
      181 tagaactatg attgactggc tacttttaat attgattttg gaggaagatt ttgtctctat
      241 tatttattat tttaaattta tctattttgt acaagatcta ttctttgaga cgggagaaat
      301 cgaaatactt gatttatacg gcctatatca tatttggggt aaatgtagta tatggtattc
      361 aatggttatt aagagaactg atttcaacta tttcccctta atgtgtatgg aaactataac
      421 aaaacgcata agattaaggt ttgtgtacct gttcttatgc gtttttgtaa tattgaagat
      481 tttcggtaag ttttatacct ttgggaagtc tagtttagaa tgctttccca accagaatct
      541 ctgcttcgat ggtaatctct gtcagttggc tccagtcaat cttgctctgg tcaacgtgaa
      601 agaggagagg agtcatactg agtagggctt ggagttgttc tgctgtgata gtcttagtca
      661 gagaggcagt ttgactagat aggatggtaa agtgttcctg gaaatgctcc ttgatatctt
      721 ggttagaata ctccttgttt gtcagctggt cctgtactct ttgacggatt tctttgaggt
      781 gattttcagt tgggataacc tttatcaaga taccgtcttt ggataaaacg cgacgaaatt
      841 ctccatagtt ggcaggtgag aagatatcaa gcagaatatc catgttagcg tcttttatag
      901 gaagtcgtgc caagtcgcca acgaaccaat tgactgccca gttgggttca cttttagcag
      961 cgatttggac tgaatctttg gagatgtcaa aggcatagaa agttttttca gagtgacttt
     1021 cttgtagttt gcgagaatag aatccttcac cacaaccgat atccaaaatt gttgtggtag
     1081 tttttgagct tgcaagcaag tcagatacag catctaagat agcttggtaa aagccggctt
     1141 ctaggatttg ttgacggttt tgaaaatttt ccttgtcata gttagcagat tgcttgattt
     1201 gaggtgctag attgacatag ccaaatttcg ccaagtcaaa agaatgacgg ttgcagcact
     1261 tgaaattagt ctctaacaga gtcagatttt cttgacagat aggacaggca aaggcagtcg
     1321 cagaagcaaa acgctgaagt ttgggcttga gatttgtatt catagtttaa gtgtatcaaa
     1381 agaggcaaat gaaagcaact ttaggaataa gtagatggga gattcagtag aaataaaact
     1441 aattctccaa tttatagaat gaaattgctt ttatatctaa attgctatat aataatgata
     1501 aggaggaaat aagtatgtta aaagaagtat taaccgttgc aaaagttgcg aaaaaatctt
     1561 cattattttt gggtggtgtc gcatttggta cccttggttt gaaaatctta gcaagtaagg
     1621 aagctaaaaa aggttattct aaagctttgg ctaaggctta caagttgaaa gacgagctag
     1681 atgcatctgt ttctgttgtg aagcaacatg gagacgatgt cttgcaagat gccaaatatt
     1741 tgtacgagca agagaaaaaa gaagagcaat tagatagcct tataggtgaa taatatgtct
     1801 tttaaagtgc tacatagagg ataccaacat atccgactat catcttcttt ttcacttacc
     1861 ttggatattc aagactatct tcgttccttg gcgagagatg aaaaggggat tgagtctatc
     1921 cagttttaca tggatcaaca gcactttact ctacgcataa aagaaggctt ttctgtatta
     1981 gataatgcag aagccttttt aaaaagaatt gataaaggga aagtttctga gttgatgact
     2041 cttcccattc gtagagaaga gagtgcttat tctattgttt caggtgcagc ggttaagcgt
     2101 gtactttttc gtggttttgt gccgtatcct attcgctata tatggacttg ttatcaggct
     2161 ttgggttata ttagagaagc ctatcaaaca ctagcgcgta aggaactaac gatggaagtc
     2221 ttggactgtt cggctatttt attgtctttg tttatgaacc aatccaagac agctagcaat
     2281 atcatgttta tgcttgattt ggggaatcat ttagatcagt ggtccttgaa aaaaactgca
     2341 acagatttag aacagagtct tcttgcaaaa gagagcgatg tattcctagt acagggcgat
     2401 acggttgtta gtatcaagag ttccgatgtt caaataggag atgtcttgat cttatctcaa
     2461 ggaaatgaaa ttctgtttga tggacaagta gtttcaggtt taggtatggt caacgaaagt
     2521 tccttgacag gagagagttt tccagttgaa aaaagagagt ctgatttggt ttgtgcaaat
     2581 acagtattag aaactggaga gttacgcatt cgtgtaacag ataatcagat gaacagccgt
     2641 attttacagc tgattgagtt gatgaagaaa tctgaagaaa acaagaaaac gaaacaacgc
     2701 tatttcatca agatggcgga taaagtcgtt aaatataatt tcttgggggc tgggctgact
     2761 tacctattga caggttcttt ttctaaggct atttctttcc tattggtcga tttctcctgc
     2821 gctttgaaaa tctctactcc tgtagcttat ttgacagtta tcaaggaagg gttgaaccgt
     2881 gaaatggtga ttaaggatgg agatgttctg gagaaatatc tggaagttga tactttcttg
     2941 tttgataaga caggaacaat cacaactagt tatcctatag ttgaaaaggt gttacctttt
     3001 ggagactata gtgaggaaga tattctcaga atcagtgcct gtcttgagga acacatttat
     3061 catcctattg ctaatgccat cgtcaagcaa gctgagatag agggaattga acatgaggaa
     3121 atgcatggga aactccaata tatcgcaagc aaggggatca aatctcatat agatggccaa
     3181 ccagttctta ttgggaatta tgtcttgatg caggatgagc agattcatat cagttcagaa
     3241 caaaatgctt taattgaaga gtacaagagt cactacaatc tcttattctt ggcttatcaa
     3301 aatgaattga ttggaatgtt ctgcattcat actcctttga gaaaagaagc aaaaacagcc
     3361 ttggataaac ttaaggcaca ggggaaaaaa ttgattctgg caacaggaga caccttgatt
     3421 aggacagagg aattagtcaa agatttgccc tttgatcagg tctatacaga cttgaaacct
     3481 gatgggaaat ttgagttagt agagaaactg cagaaagcag gtcacactat tttgatggtt
     3541 ggagatggat tgaatgactc agcggcttta actctatcag atatcggtgt ggtgatgaat
     3601 gagagtgcag atatttctaa gcagatgagt gatatcttat tgttagataa tcgtttggat
     3661 ttcttccaag agttggattc gttatcatca tctttgcaaa cactcatcaa gaaaaatatt
     3721 caagataccg ttgtcgtaaa tagtagtttg attggctttg gcttatttaa ttggctcagt
     3781 ccttcaaatc tctctatctt acataatcta acaaccttgc gcattgtctt gcgtagcctg
     3841 tctattaaaa atagatagat gagagttatt aacagcaaaa aggagttacc tttctcgagg
     3901 ttaactcctt tgtttataga taaatgttga atagtttagt aagtcaatac aaagtatttt
     3961 ttcttcccac gacggataac agtcagttca ttttctaact tatcagcgtc actcaagaca
     4021 tagtcaaggt cttggatgcg gtcgccgttt acgtagatgg ctccgttttg gacgtcttca
     4081 cgggcttggc gttttgagtt aactatacca gatgagacga gcagttccac gatattgttg
     4141 tgttcgtctg cctgtacttg gtagtttggc acaccacgaa gtccttgttt gagctctttg
     4201 acagaaaggt ttttgatgtt tcctgcaaag agttgctcag tgatgttaag tgcttctttg
     4261 taggattctt ctccgtgaac aagtgtaaca acttcacgag ccaagacttt ttgagccaag
     4321 cgttcgtgtg gcgctgcttc aaattgtttg cgaatatctt caatctcatc aagtgacaag
     4381 aaagtaaaga ttttcaagaa gcgaacagcg tcagcgtcca tcacgttcat ccagaattgg
     4441 tacatttcgt atggagaagt cttttcggga ttgagccaga cggcatttcc ttctgattta
     4501 ccaaatttct taccagttgc atctgtgatt agtggaacag tgataacgtg accagtcttg
     4561 tccgccttac gacgaagcaa ttcggtacca gctgtcatat ttccccactg gtcagaacca
     4621 ccgatttgaa gagtgacatt atggtcttgg ttaaggacga agaagtcata cccttgcatg
     4681 atttggtaag cgaactcagt gtaagaaatt cctgtttcga tccgtttttt aacagattcc
     4741 ttactcatca tgtagttgac cgtgaagtat tttccaatat cacggaggaa gtcaatgaag
     4801 ctgatgctgc caaaccagtc gtagttgttg accatgacag ccttgttttc gccattttca
     4861 aagtcaagaa aacgagaaag ttgtccttgg atagacttga cccagccatc tactgtgtct
     4921 tttgtttgga gactacgttc agcatctttg aaggacggat ctccgatgag acctgtagca
     4981 ccgccaacga gcgcataagg tttgtgacct gctagttgca agcgacgact tgtcaagatt
     5041 gcgacaaggt ggcctaggtg aaggctgtca gcagttggat cgtagccagt atagtaagaa
     5101 acttgacctt cttctagggc tttacgcaaa gcttcttcat cagtcgtttg aaatatcaaa
     5161 ccacgctctt ttagctcatc aaaaatgtgc atgtgtcttt tctccttttt aaaattattg
     5221 tttctaccta ttgtatcaca aacttgggca atagcctagc agaataggga agaaagtggc
     5281 tattttattg aaaatttccc ttttgtggta taatagatag agtgaggaaa tccatgcaaa
     5341 atcaattaaa tgaattaaaa cgaaaaatgc tggaattttt ccagcaaaaa caaaaaaata
     5401 aaaaatcagc tagacctggc aagaaaggtt caagtaccaa aaaatctaaa accttagata
     5461 agtcagccat tttcccagct attttactga gtataaaagc cttatttaac ttactctttg
     5521 tactcggttt tctaggagga atgttgggag ttgggattgc tttggggtac ggagtggcct
     5581 tatttgacaa ggttcgggtg cctcagacag aagaattggt gaatcaggtc aaggacatct
     5641 cttctatttc agagattacc tattcggacg ggacggtgat tgcttccata gagagtgatt
     5701 tgttgcgcac ttctatctca tctgagcaaa tttcggaaaa tctgaagaag gctatcattg
     5761 cgacagaaga tgaacacttt aaagaacata agggtgtagt acccaaggcg gtgattcgtg
     5821 cgaccttggg gaaatttgta ggtttgggtt cctctagtgg gggttcaacc ttgacccagc
     5881 aactaattaa acagcaggtg gttggggatg cgccgacctt ggctcgtaag gcggcagaga
     5941 ttgtggatgc tcttgccttg gaacgcgcca tgaataaaga tgagatttta acgacctatc
     6001 tcaatgtggc tccctttggc cgaaataata agggacagaa tattgcaggg gctcggcaag
     6061 cagctgaggg aattttcggt gtagatgcca gtcagttgac tgttcctcaa gcagcatttt
     6121 tagcaggact tccacagagt cccattactt actctcctta tgaaaatact ggggagttga
     6181 agagtgatga agacctagaa attggcttaa gacgggctaa ggcagttctt tacagtatgt
     6241 atcgtacagg tgcattaagc aaagacgagt attctcagta caaggattat gaccttaaac
     6301 aggacttttt accatcgggc acggttacag gaatttcacg agactattta tactttacaa
     6361 ctttggcaga agctcaagaa cgtatgtatg attatctagc tcagagagac aatgtctccg
     6421 ctaaggagtt gaaaaatgag gcaactcaga agttttatcg agatttggca gccaaggaaa
     6481 ttgaaaatgg tggttataag attactacta ccatagatca gaaaattcat tctgccatgc
     6541 aaagtgcggt tgctgattat ggctatcttt tagacgatgg aacaggtcgt gtagaagtag
     6601 ggaatgtctt gatggacaac caaacaggtg ctattctagg ctttgtaggt ggtcgtaatt
     6661 atcaagaaaa tcaaaataat catgcctttg ataccaaacg ttcgccagct tctactacca
     6721 agcccttgct ggcctacggt attgctattg accagggctt gatgggaagt gaaacgattc
     6781 tatctaacta tccaacaaac tttgctaatg gcaatccgat tatgtatgct aatagcaagg
     6841 gaacagggat gatgaccttg ggagaagctc tgaactattc atggaatatc cctgcttact
     6901 ggacctatcg tatgctccgt gaaaagggtg ttgatgtcaa gggttatatg gaaaagatgg
     6961 gttacgagat tcctgagtac ggtattgaga gcttgccaat gggtggtggt attgaagtca
     7021 cagttgccca gcataccaat ggctatcaga ccttagctaa taatggagtt tatcatcaga
     7081 agcatgtgat ttcaaagatt gaagcagcag atggtagagt ggtgtatgag tatcaggata
     7141 aaccggttca agtctattca aaagctactg cgacgattat gcagggattg ctacgagaag
     7201 ttctatcttc tcgtgtgaca acaaccttca agtctaacct gacttcttta aatcctactc
     7261 tggctaatgc agattggatt gggaagactg gtacaaccaa ccaagacgaa aatatgtggc
     7321 tcatgctttc gacacctaga ttaaccctag gtggctggat tgggcatgat gataatcatt
     7381 cattgtcacg tagagcaggt tattctaata actctaatta catggctcat ctggtaaatg
     7441 cgattcagca agcttcccca agcatttggg ggaacgagcg ctttgcttta gatcctagtg
     7501 tagtgaaatc ggaagtcttg aaatcaacag gtcaaaaacc agggaaggtt tctgttgaag
     7561 gaaaagaggt agaggtcaca ggttcgactg ttaccagcta ttgggctaat aagtcaggag
     7621 cgccagcgac aagttatcgc tttgctattg gcggaagtga tgcggattat cagaatgctt
     7681 ggtctagtat tgtggggagt ctaccaactc catccagctc cagcagttca agtagtagtt
     7741 ctagcgatag cagtaactca agtactacac gaccttcttc ttcaagggcg agacgataat
     7801 tctctaaatg aagtggccaa tcaatggatt gccacttttt tcctttcgtg ttacaataag
     7861 ggtatgaatc agtatcagaa aaagattgtt aacggaaaaa tttattcgct cctatccggc
     7921 ttaatatggg gaatctgtgg aattttagga gagtacttct ttactcatta tcaggtgtct
     7981 tcgggctgga ttacctctat gcgtttgaca ctggcaggga gtcttgtact tatttggtct
     8041 gcaatacaat taaaatcgca agtgctagat atttggcgag acaagaaaaa ttacctgccc
     8101 tttttagcct atgctatttt ggggattttt tcagttcagt attttttcta tctctgtgta
     8161 gaatactcaa atgctacgac agcaactatt ttacagttta ttagccctgt ctttatcctc
     8221 ttttacaatc gcttggttta tcaaaaacga gcgtcaaaaa gcgctgtttt ctatgttttg
     8281 gttgccatgc tgggtgtttg cttgatggcg acaaagggag atctctctca gttatccatg
     8341 acgccgctag ctcttataac aggtttgctg agtgccatgg gtgttatgtt taatgttatc
     8401 ttgccccaac cttttgctaa gcgttatggt tttgttccta cggttgggtg ggggatgatt
     8461 ttggcaggtt tgtttagcaa tgtcctctcg ccggtttatc agctttcctt tactcttgat
     8521 atttggagta tcttgatttg cctcattatc gctttctttg gaacggcttt tgcttttttc
     8581 atttccatga aggctgtgtc cttggtttct cctttggtgg tttccgttat cagtgccagt
     8641 gaacctctct cttctgctct cttgagtgtt ttgttcttag gattagtagt ggattggtcc
     8701 ttccttctag ctatagcctt gattatttta cccatgatat ttttgtctat agaagaagcg
     8761 aaagaaagta gataaaaagt cttttcttaa ttctaaaagt gtgctatact agaatagtca
     8821 ataaaacacg gggagatagt tgtgaagagt gtttttaggt tgtatcggta ggggcttgct
     8881 tttaccgaca gcacgtttta tctcacatcc ctaaaaaatc atacctaaaa acagacaaaa
     8941 aggtttcaaa tttgaggcct tttttggtag aataggtatc attataacga accaggaggc
     9001 acctatgact gctacaaaaa tgaacgctca agaaattatc caatttatcg ccaatgctga
     9061 aaagaaaacc agtgtcaaag taacctttga ggggcaactc gcaactgctg tgcctagctc
     9121 tgttgtcaaa ctagggaatg tcctattcgg ggattggaag gacgtggctc cgcttcttga
     9181 aggtttagta gaaaatcaag attatgttgt cgagcaagat gctcgtaatt ctgcagttcc
     9241 tttgctagat aagcgtgcta tcaacgctcg tatcgagcca ggtgctatta tccgtgacca
     9301 ggtggaaatt ggtgacaatg ttgttatcat gatgggagct gttatcaata tcggtgctga
     9361 aatcggtgct ggaaccatga ttgacatggg tgccatcctt ggtggccgtg ccatcgttgg
     9421 aaaaaatagc cacgttggtg caggtgcagt tttggcaggt gtgattgagc cagctagtgc
     9481 tgaaccagtc cgtgtcggag acaatgttct catcggggct aatgcagtgg ttatcgaagg
     9541 agtccaaatc ggtagtggtt cagttgtcgc agcaggagct attgttaccc aagatgtccc
     9601 agaaaacgtg gtagtagcag gtgttccagc tcgtattatc aaagaaattg atgcccaaac
     9661 tcaacaaaaa acagcgctag aggatgcgct tcgtaccttg taattgtaaa agtaaaaaag
     9721 aggcggaacc ctttttccag cctctttctg ctatatcgga ggacagatag atgttagatt
     9781 tgattcagac tagacgagat ttacaccaga ttccagagat tggcttggag gagttcaaga
     9841 ctcaggctta tttgctggat gtgattgaga aattgactac gggcaaggat tttgttcaaa
     9901 ttcgtacttg gcggacaggg attttggtct acctgcaggg aagtcagccg gagcgaacca
     9961 ttggttggcg gacagatatt gatggcctgc ctatcgtcga acaaacaggt ctaccttttg
    10021 cttctcaaca ccaaggtcgc atgcatgcct gtggccatga ttttcatatg accattgcct
    10081 taggctgtct tgagcgcgcc cttgaggagc aacctaaaaa taatctgctc tttctatttc
    10141 agcctgctga agaaaatgaa gctggtggta tgctcatgta tgaggctggt gcttttggag
    10201 attggttgcc agatcaattt tatggcctcc atgtccgtcc agatttgaaa gtcggccaga
    10261 ttgcgactaa tactcataca ctctttgcag gaacttgcga ggtgaagatc cgtttcaaag
    10321 gtaaaggtgg ccacgcggct tttcctcatg aagccaatga cgccttggtg gcggctagtt
    10381 actttgtgac ccaagtgcag tcagttgtta gccgcaatgt caacccaatc gagggagcag
    10441 tggtgacctt cggcgttttt caagctggaa ccaccaacaa tgtcatcaca gacacagctt
    10501 ttttacatgg aactattcgt gccttgacac aggacatgag tctcttggtg caaaagaggg
    10561 taaaaacagt tgcagaaggg gttgcagctg cctttgatat ggaagtcgaa gtggaactca
    10621 aacaaggtgg atacctacct gttgagaaca atccagcctt ggcgcgtgaa ctgatggact
    10681 tctttgacga aaaagacgga atcgagttga ttgatatcga acctgctatg acaggcgagg
    10741 actttggtta tctcctttca aaggttgatg gcgtgatgtt ttggctaggt atcgatagtc
    10801 cctacgctct tcatcaccct caaatgagtc ctaaggaaga agtcttagct attggggtgg
    10861 ctgcggtgtc tagtttcctg aaaaagaagg cagcagaata gagggcttgt ctatgaaatc
    10921 ggaattacgc aagcaagtct tgcaaggaat gaaggctata tctcaagaac aaaaacagtt
    10981 tatagatcaa actttaaccg agcggctttt acaccatccc ttctatcaag aagccaaggt
    11041 catcgcaacc tatctctctt ttcctcatga gtttcaaacg caggaactga ttgagcaggc
    11101 gctgaaggac ggcaagaagg ttttaatacc caaaacttat cccaaggggc gcatggactt
    11161 tgtggtctat gatccgcaac agttggtaaa aacttccttt ggattactgg agccacaggg
    11221 agatttggaa gtggtggatg catctcagat tgatttgatt catgttcctg gcctggcttt
    11281 tacgacaaaa ggatatcgga taggatacgg tggaggctat tatgaccgct atctggaaca
    11341 tttttctggt catactttga gtacggttca tccttgtcaa attcaggact ttatacctga
    11401 aaaccatgat attcctgttc aggaggtatt aattgatgaa ggaaatcttt gatagacgtt
    11461 accctgtgac gagtttcttc ctcttagtga cggccttggt atttttacta atgttggtca
    11521 ctgcaggcgg aaactttgac agggcagata cattatttcg atttggagcc atgtatgggc
    11581 cagctattcg cctctttccc gagcaggttt ggcgtctctt gtctgccatt tttgttcata
    11641 ttgggtggga acatttcatt gttaatatgc tttcacttta ttatcttgga aggcaggtag
    11701 aggagatttt cggttctaag cagtttttct ttctctatct tttatcagga atgatgggca
    11761 atctctttgt ttttgtattt agtcctaaat ccttagcagc aggcgcctct acctctcttt
    11821 atgggctatt tgccgcgatt atcattcttc gctatgcaac tcgcaatcct tatatccaac
    11881 agctagggca atcttatctg acactttttg tggttaacat tattggaagt gttctgattc
    11941 caggaatcag cctagcaggc catatcggtg gtgcagttgg tggcgcattt ctagcagtta
    12001 tctttccagt tagaggagaa aaacggatgt ataacaccag ccagagatta ggagcggtag
    12061 tcttgttcgt aggactcgcc attttgcttt tctacaaggg aatgggaatg tgacgaagtt
    12121 gaaagaaaat ttgtgtaatg aaaaaagata aggcattttt tgaaccttat ctttttattt
    12181 tattccttct cagtcaattc ttttccaagt tggatgaggt aattcttcaa atcatctttg
    12241 acttgtggat gtttgagggc gtagtcaata gatgttttca taaagccaaa cttatcccca
    12301 acgtcgtaac gagctcctgt aaattcacgg gcaaaaacac gctgtgtttt attaagagta
    12361 tcgattgcat cagtaagttg gatttcattc ccagcaccag gagcttgctt ttcgagaatc
    12421 tcaaaaattt caggcgtgag gaggtagcgt ccgataatag caaggtcgct aggagcgtcc
    12481 tctggagctg gtttttcaac aaaggtttca acactgtaaa gaccatcttt tccttcgcct
    12541 tgcggagcaa taaccccgta agcagatact tcgtcatgag ggactggcat gacagcgata
    12601 gtagacgcgt gggtacgctc gtagtcatcc atgagttgtt tggtaagtgg aacagccttt
    12661 tcgtctgtga tatccatcaa gtcatcacca agcataacga caaaaggttc atttccgacg
    12721 aaagccttgg cttgcaaaac agcatctccg agaccgcgtg gatgagtttg gcggataaaa
    12781 tgcagacgca tgccagttgt ttcatcaact agcttcaaaa gatctgtttt ccctttttct
    12841 ttgaggttat attccaattc gaagtttgaa tcgaagtggt cctcaataga acgttttgat
    12901 ttaccagtga caactagaat atcttcaata cctgatttga gagcctcttc cacgataaac
    12961 tggatagttg gtttgtctac gattggcaac atttctttgg caagggcctt ggttgctggt
    13021 aaaaatcgag ttcctagtcc agcagcaggg ataactgcct ttctaacttt tgatgtcata
    13081 aaaatccttt ctatagaggg ttaagaccac tcattttctg ctttaaattc attgttcatg
    13141 atgtcataaa tggcatcttt gatattggtt ccgtgataaa taacttggta aatagcctgt
    13201 gtaatgggca tatagactcc aagttcttgg gctagttcat aggctgctcg agtcgttgaa
    13261 attccttcga ttaccatgcc catattagct tctatatcag ctagggattc tcctcgtccg
    13321 agagcatctc cagctctcca gttacgagag tggatggaag ttcctgttac gattaaatct
    13381 cccacaccag ataagccgct ataggtcaat ggactggccc cgagtgctac ccctaggcgg
    13441 gtgatttctg ctaaacctcg agcgatgatg gctgccttag cattatcacc aaatccaaga
    13501 ccatgtaaag ctccagcacc gacagcaata atatttttaa gagcaccagc agtttcaacc
    13561 ccgataacat ccgtattggt ataaagtcgg aagtagtgat tactaaatag ctcctgaacg
    13621 tattgagctg tttgtaaatc tttagaagca gcagttatta aagttaggtc acgcacaatg
    13681 gtctcttctg catgactagg ccctgaaaca acgacgatat cactacggag atgttcagga
    13741 atttcttctt caagaatggt tgataatcgt ttatggctat caggttctaa tccctttgat
    13801 gcgtgcatga tgataacctt atggtccaag gtttgtgcaa cttgctgggc aacaagtcgt
    13861 gtcacttttg ttgggacaac aaacaaaatc gcatccacat ttttcaatgt ttctgctaag
    13921 tcggtgtagg caatgatatt ttcgtctaga acgacatctt taaagtagtg cttattagta
    13981 tggtgtgtat taatttcatt gatttgctcg ggaagatttc cccaaatacg tacctcgtgt
    14041 ccattgtcat ttaagacttg tgaaagggcg gttccccaag aaccaggccc caagacggcg
    14101 acggtttgtt tttccatgat ttcctccttg atagagttct ttctcttatt ctatcatatt
    14161 ctaggggatt ttgcacttgc attactgggg tatggtggaa aaatacacaa aaaccgagat
    14221 gaagggataa actcttagtc tttagttaaa gtgagaagaa atctgataga tagtttgatt
    14281 cagtatatgc tataaataga ggtaaacaac tcaggaggtt cttgtaggtt gcgagagttt
    14341 ggcgaaaaaa ttaaaagatt acgtttggct aaaaaaatca gtcgttcaga attttgtggt
    14401 gatgagtctg aattaagtat ccgtcaatta attagaattg aaaatggaga atccagacca
    14461 acactaacaa agttaaaata tatcgctgaa cgtttggggg ttgaagatta caagttgatg
    14521 ccaagttata tagagttgga taaggaatac ctagaattga agtatttctt gatgaggact
    14581 cctacatacg aagatgaaac tatcgcccaa aagaaagaga gcgttttgat aagatttttg
    14641 aagagtatta tgataggcta cctgaggaag aaagatttat catcccaaat tattcatatc
    14701 tggcactaac gaactacaca gttcaaaaat taccagaaaa gctagttgaa atactgtcct
    14761 tctggtgatt ttgcttaatt tctgatgttc gagacatgat tgaggagatt tgtcttgatt
    14821 ttttcctaaa aaagggcaga ctcctccctt ggtttgatgc tcgattttta aagtaaggtg
    14881 acttattctg ttaggcatgt tttcccctgc cctctagctc tatatggaac agggagtatc
    14941 agatttatct ggcagattgt atcaaataag gcttaaaact gttttgaata ggcatataga
    15001 cttgagaatt tgagaatatg tcgtctagca gtagagacat attttccggc aatatgaagg
    15061 aagagacgtc gaaaacgctt gatagtcaag gcctttactt catcaccagc tagctgcttt
    15121 aaaaagaggt agagattata agccagacag cccatcatca tacgtacttc attcttaatc
    15181 atggtcgaac tatctgtctt atccccaaag aatcctgctt tcctttcttt gataaagttt
    15241 tcagcattcc ctcgttcacg atagaattga aacagctcct cactatctac actgcttaaa
    15301 ttgttcaaat gaactttttt acaaccataa aaaagagcac ttaatcgtac tcttctcttt
    15361 taaggattag ttgaattaat tcaaatccac tagttctcct gtttctaggt agactaggcg
    15421 ttcacaaatg ttagcaatgt aatcagcgaa gcgttccaga tgccctatga tataaaggta
    15481 ttgtgttcca ttgggaattg aggtttcttg gtctttcata agtccaatga tttcctttga
    15541 taaggcataa taatattggt caatctgttc atctttttga gcaatactaa tagcttttga
    15601 ggcttggtgc aaaggaaagg caaccaataa atcggctagc atgctgaggg ataatttacc
    15661 catttggtgt aactgttctt cgtcaggggc tagttgattt tcttttagtt gcaaaacagc
    15721 tttggcaatg cctgccatat ggtctcccat acgttcaagg tctgaacaag aagacatgat
    15781 gctaattaca aatcgaaggt cagacacttg tggctgctgc aaggctaaca aacgggcaca
    15841 ggccaattcg atagcgcttt gaccttggtt gatagcatga tccttattga taattagctc
    15901 tgccatctcc ttgtctttgg aggctaaggc cagtaaggct tttgaagctg tttcaaggac
    15961 aagttgccct agtcctaaaa aggattgttc taattcatgc aattctaagt caaattgatt
    16021 tctcataggt ttttccttcc ttatccgaac cgtcctgaaa tatagtcttc tgtgcgctga
    16081 tcttttggat tggtaaacac gtcaacggta tctccaaatt cgcaaatttc tcctgttaag
    16141 aaaaaagcag ttttatctga aatacgtgaa gcttgttgca tgttatgggt aacaatgata
    16201 atcgtataat ccttttttag ttgctgaatg aggtcttcaa tttttaaagt ggagataggg
    16261 tctaaggctg aagtcggctc atccattaac agaatatcag gttctactgc taaagctcgc
    16321 gcaatgcaaa ggcgttgctg ctgaccgcca gataaggaca tggcactctt tttaagatca
    16381 tctttgactt cttcccaaat ggctgcccct tttaaagatt tctccactaa ggcatctaat
    16441 tgttttttgt ctcgaatacc atgtgtcctt ggaccataag ccacgttatc atagatagac
    16501 atggcaaagg gattaggctg ttgaaaaacc atccctacac gcttgcgtag ctgattaagg
    16561 ttgaatttgc tactataaat atcttgctca tctaagagga cttggccttc aatatggcaa
    16621 gaaggaacca aatcgttcat ccgattaagg gtttttagaa aagttgattt gccacaacca
    16681 gatgggccta tcaaggcagt aatctgtctt tctggtaatt gaatcgaaat attttttaag
    16741 gcttgaaaat ccccgtaaaa taagtctagg tgtctaactg aaaatgttcc catactagga
    16801 agctcctttc acaagtttcc gagataataa gcttgataga gtatttatca ttaaaacagt
    16861 aataatcaaa atcacgccgg tagcataggc ttcattgaca tgtagcccct cacttgacag
    16921 catatacata tgtagggcta gagaacggcc tgaagacatg agactacttg gcgtattggt
    16981 agaggtacct aatgtataca tgagggcagc tgtttcacca acgatacggc caatagctag
    17041 tatcactcca gctaaaatac ctggcatggc aactggtaga acaattctaa aaacagtccg
    17101 taacttacct gccccaagtc cataacttgc ttgacgcata ctatcactaa cagataaaag
    17161 ggcttcttct gttgagcgaa taatgactgg caacaccatg ataactgagg ttaagattcc
    17221 tgataacaga gagtattgaa aacctaagaa gactacaaag aagagcatgc caaacagacc
    17281 aaaaacaatg gaaggaatcc cagataaggt atctgaggcc aatcgcatga ttttaacaca
    17341 aagggaatct ttttttgtat attccacaag ataaaaacca gcaaaaatcc ctatgggcaa
    17401 ggctaaaaga agagcaccaa agaccagaat aacggtggaa ataatcgctg gcataaggga
    17461 aatgttctca gaagtataag tccaagaaaa gagggataga cttagatgag gtaagccttt
    17521 gatgaggata aaaccaatga ttaaaaagag agagccaaag gttaaagctg aaaaacaata
    17581 aacgagaagt tttagcaggt atttactcat aagatgattt tcctttcaag taggcaaagt
    17641 aggcattaat caagagaata aggaaaaaga gaactgctga ggttgcaata agggcttccc
    17701 tatgctgacc tgatgcgtaa gccatttcca gaacaatatt ggttgttaag gttctggttc
    17761 ctgaaaagag tccacttgga ataatcggct ggttgcctgc caccaaaata actgccatgg
    17821 tttcacctac tgcgcgaccg attcctaaaa taactgctga taaaatacca gatctcgcag
    17881 ctggcaagat gacactaaaa atactccgtt catgactagc tcctagagcc aagctaccag
    17941 aataatacgt tttgggaact gttcggatag cagattctga caaactgata atggttggca
    18001 aaatcattat tcctaatagt aacgaagcgg ttaggacact catgccattt cctaaaaagc
    18061 ttctaatcca aggcaccaat aattgtaggc cgaaaaaacc ataaacaata gatggaatgg
    18121 ctgccatcaa gttgatagct gattttaaga agccatagac gggctttgga caataataaa
    18181 ccataaacac cgatgtcaag atgcctgttg gcaccccaat cacaatcgct cctaaggtaa
    18241 ttaataagga accaacgatc attggtaaaa taccatagct tgccggaatg ttcgttggcg
    18301 accaatcact gcctaataaa aaacgggcaa agccgtagtt agctatgaaa ggtaagccat
    18361 tactaaaaat aaagaaacag attagcaaaa tagctacaac agctactgtt gcactcatga
    18421 aaaaaattgc cctaaaaact gcttctttga aggcttgttt tgtcacatct tgtcctttct
    18481 agtgaagaaa gtaagggaga tacgacacct ccctacttgc cttctttatc ttattgtacg
    18541 atgaaacgtc tgcatctctt tagagattta tggagcaaac attttattta atcttgtccc
    18601 aggtggttaa tttgccacta aaaacgtctg caagttcagc catactgact tggcttgcct
    18661 tattgtcatt attgaccaca acagcaatac cgtctaaagc aatagcatca tgggtgagac
    18721 tcttaccttc ttcaggagtt aattccctag aaaccatacc aatatcagcg gttttctcct
    18781 taacagcggt aatacctgct gaagacccat tagaggtaat atcaatcgta acttctggat
    18841 tttctttttt ataagcttct gctaattttt ccattaaaga agatactgaa gtggaaccta
    18901 caacagacaa cttgcctgat aagtgttggc ttgtatattc tgtggtttcg gttttagctt
    18961 caataaattt attatctgtg accacttgtt gaccttgttt ggagtggata aagctgataa
    19021 aatcttgacc tagcttggaa agattagaag accaaacaat gttgaaggga cgttgaagag
    19081 ggtattcacc atctaaaact gtgtctcgac tagccttgac accatcaatc tctaaagcct
    19141 tgacagattt cgttaaagat cccaaggaga tgtagccgat agcattagca ttcccttgaa
    19201 ctgctgagag aacaccttct gtactatttt gaatcacagc tgttttggca gtgttgtcaa
    19261 tttttttatc accgtctttt ttgagaatcc ctgtgatttc tgtgaaggca ccccgtgttc
    19321 cagagccatt ttctcgtgaa atcacctcaa tcgttcctga agctgactgt ttggaagcag
    19381 ctgactgatt gccacaggca acaagcccaa atcctgataa gccaatggct gcaagagtaa
    19441 gcattttttt gaatttcata ataatcacct ttatctctat gtatttttct tgtgtaggct
    19501 tactacattt atagtttaac aagtctttgt aaaggtttat ccctgattca tgtaaagatt
    19561 gtgtaaagaa tcaaaaaaag ccacttttga aaaatggctg ctcctaaaaa tagctttaaa
    19621 aattattagt cctgtgcgaa agattggtta ggaagaaaaa tcgtgaagca actgcctctg
    19681 ccaagctgac tcgtcaccgt gacttggcca cctaataatt gactgagttc tttgacaatg
    19741 gcaaggccaa gaccagtgcc accagtttgt ctgcttcgac ctttattaac tcggtaaaaa
    19801 cgttcaaaaa tacgatcctg ctctaattga ctaataccaa tccctgtatc tgatacagaa
    19861 atcttaatgc cttcgttcac cttttgggtc ttgacctcaa tttttccccc ttgttcagtg
    19921 taacggatgg cattggataa aagattgagt aagatttggg aaagtaattg actatctgat
    19981 acgagggtga catcatctgg cacctgcacc tttagctgta aatccttctt cttgagctga
    20041 ggttgcaagc tttgagtcaa atcctgtaca aattctgcca aagaaagggt cgtccattgt
    20101 ataggcattt gttgagcctt agataaggta agaagatgct caacaatatg ctcaagacgc
    20161 aaactttctt tgtaaataat gtctagaaag tcatccttga gcgcttcttc ttcagctgac
    20221 atccccttaa tggtttcagc aaagccctta attgaagtaa ctggtgtcct caattcatgg
    20281 gaggcatttg agacaaaggc taaatttaac ttttcataag ttctaatcgt tgttaaatca
    20341 tatagcaaga cgagcacagc ttccacagat tgggtggggc taaaaacggg aactgctgtc
    20401 acttctaaaa tcaagtcacc ctcatgaaac ccacttactt cttgttttaa ccttgttttt
    20461 tgatcaaagg cttggtgaac taaattccga atatccatcc gtttgaggtc atcaagtgaa
    20521 cttatgtcgc cgtccacatc gggaaaataa tgaggcagag agcgactgga taataacatc
    20581 tgaccttgag cggaaactaa aaacgtcccc atggttaggt gcgacagaag aacctccatt
    20641 gtttcggcta ggtccttgta ttgctgatcc tgttgggaga ctttggtttt taggccagac
    20701 acatactgag ccaaagactt taagtcttct tgcccttttt ctaaaaagta ttcactactg
    20761 gtcaagagag gttggtgcaa ggtctcaaaa gcaacttccc atttccaaag gcaaaagagc
    20821 cagtagccac cttgtcccaa agaaagggct agaagaaaga gaccgatgcc tttactgatc
    20881 caagttaatg ccatccctgc aatcagaatg aggctaacac ttagattgac tagccaaaat
    20941 tgaaggtagc gtttcatcta taactccttg aacttataac cataaccccg aatggttcga
    21001 ataaattgag gggctttagg attgtcttca attttttccc tcaacttacc aatatgaacg
    21061 tccaccaaac gtgtttcctg cccaaagtca tacccccaga tacgttccaa aagacgctct
    21121 ctagtcagtg tcatgttggg atgtttcata agatagagca agagttcaaa ttcttttggg
    21181 gtcaaactca gtaacttatt cgccttgtag acttcatgac gctcagggta tactttcaag
    21241 gtcccaaata gccaagaatc gtcagcgata ttatctgaat catctccttc ttgttctcct
    21301 ttagttcgcc tgaggacagc cttgacacgc gccagcaatt ctctagggct aaaaggcttg
    21361 gtcaggtagt catcagcccc taattccaag gccaaaacct tatcaaattc atcacttttc
    21421 gcagaaacca tcataattgg agttttgacg cctttggctc tcagccgctt acaaacttcc
    21481 atgccatcta attgtggtaa catgatatca agcaagataa aatcaaaggg ttctgtttct
    21541 gccaaagcta aggccttccg tccatttgtt accaattgag tagaaaagcc ttccttactt
    21601 aaatggtagt caagcaattt cagaatgtgt tcttcatcat ccactaataa gacttgtttt
    21661 gtcatctatt atctcctatt ggtaacatta taacacaatt atcagaaatc ctaacattgc
    21721 taaatcagat taaatttgct tatcaagact agtatctggt caaacgctca atcatctcct
    21781 tgtgctctgg ataggtcgcc agtagatcta ccctttcaaa taattcaaaa tcctcaaatt
    21841 caaaaccagg agcaacaaga caagaaacca gagcataatc cttatcaact gttgatcccc
    21901 aaatagtgcc cttaggaaca cagtagtgaa gttgttgccc tttggatatg tccaggccta
    21961 aagtgactac ttcgtagtga ccatctgctg taatcatgtg aacagtaagt ggggatcctg
    22021 catgaaaata ccagatttca tctgctgtca atcggtgaaa atgtgaagga ttcgtttctt
    22081 cttaataaga aataaatact ggtataaagc gcccttccct taccagcaag gtttatagtg
    22141 tctgaagctt tttttgtttg tctaaaatag ccaccttcaa catggggagc taactctaga
    22201 gttcttatca agtcttcttt atccgtcgga gccaatgggt tgaagtaact cttgttcaaa
    22261 gtggtgttac g
//
LOCUS       DBERMR010000034        22207 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.034, whole genome shotgun sequence.
ACCESSION   DBERMR010000034 DBERMR010000000
VERSION     DBERMR010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 22207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22207
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.034"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..240
                     /locus_tag="ACL8A1_001412"
     CDS             <1..240
                     /locus_tag="ACL8A1_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HGS1202167.1"
                     /translation="PEKPKPDNSKPQADDKKPSTTNNLSKDKQPSNQASTNEKATNKP
                     KKSLPSTGSISNLALEIAGLLTLAGATILAKKRMK"
     gene            complement(459..1439)
                     /gene="dusB"
                     /locus_tag="ACL8A1_001413"
     CDS             complement(459..1439)
                     /gene="dusB"
                     /locus_tag="ACL8A1_001413"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HGS1202168.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKV"
     gene            complement(1426..2298)
                     /gene="hslO"
                     /locus_tag="ACL8A1_001414"
     CDS             complement(1426..2298)
                     /gene="hslO"
                     /locus_tag="ACL8A1_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HGS1202169.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            2463..3890
                     /locus_tag="ACL8A1_001415"
     CDS             2463..3890
                     /locus_tag="ACL8A1_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS1202170.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELTIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLFY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSVGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLVAIRQLLFT"
     gene            4049..5557
                     /gene="glpK"
                     /locus_tag="ACL8A1_001416"
     CDS             4049..5557
                     /gene="glpK"
                     /locus_tag="ACL8A1_001416"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HGS1202171.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYIVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEIDD"
     gene            5704..7530
                     /gene="glpO"
                     /locus_tag="ACL8A1_001417"
     CDS             5704..7530
                     /gene="glpO"
                     /locus_tag="ACL8A1_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HGS1202172.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLVDTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            7600..8304
                     /locus_tag="ACL8A1_001418"
     CDS             7600..8304
                     /locus_tag="ACL8A1_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HGS1202173.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTIGVALKGGLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            8601..8804
                     /locus_tag="ACL8A1_001419"
     CDS             8601..8804
                     /locus_tag="ACL8A1_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202174.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEDKKQELLNRLNK"
     gene            complement(8891..9478)
                     /locus_tag="ACL8A1_001420"
     CDS             complement(8891..9478)
                     /locus_tag="ACL8A1_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202175.1"
                     /translation="MFVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNILSDEVLE
                     VVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVNTIHGNKQ
                     ANTFANFMTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSPYPYYITS
                     TYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
     gene            9632..9823
                     /locus_tag="ACL8A1_001421"
     CDS             9632..9823
                     /locus_tag="ACL8A1_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202176.1"
                     /translation="MLNAKIFYIFLCLIVIETTGLLLLKRGLEMGYLDTEAIILAVFS
                     FAFYNLCSFAWVCSTIKKQ"
     gene            10591..11850
                     /gene="ltrA"
                     /locus_tag="ACL8A1_001422"
     CDS             10591..11850
                     /gene="ltrA"
                     /locus_tag="ACL8A1_001422"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HGS1202177.1"
                     /translation="MSKLLDKILSRENMLEAYNQVKSNKGSAGIDGMTIEEMDNYLRQ
                     NWRLTKELIKQRKYKPQPVLKVEIPKPDGGIRQLGIPTVMDRMIQQAIVQVMSPICEP
                     HFSDTSYGFRPNRSCEKAIMKLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     EDGDTESLIRKYLHSGVIINGQRYKTLVGTPQGGNLSPLLSNIMLNELDKELEKRGLR
                     FVRYADDCVITVGSEAASKRVMYSVSRFIEKRLGLKVNMTKTKITRPRELKYLGFGFW
                     KSSDGWKSRPHQDSVRRFKFKLKKLTQRKWSIEQLNLSIRGWINYFSLGNMKSIVASI
                     DERLRTRLRVIIWKQWKKKSRRLWGLLKLGVPKWIADKVSGWGDHYQLVAQKSVLKRA
                     ISKPVLEKRGLVSCLDYYLERHALKVS"
     gene            12306..13484
                     /locus_tag="ACL8A1_001423"
     CDS             12306..13484
                     /locus_tag="ACL8A1_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ryptide export MFS transporter"
                     /protein_id="HGS1202178.1"
                     /translation="MSNLFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFTAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTFIILVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEISSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            13798..13929
                     /locus_tag="ACL8A1_001424"
     CDS             13798..13929
                     /locus_tag="ACL8A1_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HGS1202179.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            13950..15500
                     /gene="dltA"
                     /locus_tag="ACL8A1_001425"
     CDS             13950..15500
                     /gene="dltA"
                     /locus_tag="ACL8A1_001425"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HGS1202180.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMKNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            15497..16741
                     /gene="dltB"
                     /locus_tag="ACL8A1_001426"
     CDS             15497..16741
                     /gene="dltB"
                     /locus_tag="ACL8A1_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HGS1202181.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMRGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            16755..16994
                     /gene="dltC"
                     /locus_tag="ACL8A1_001427"
     CDS             16755..16994
                     /gene="dltC"
                     /locus_tag="ACL8A1_001427"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HGS1202182.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            16987..18255
                     /gene="dltD"
                     /locus_tag="ACL8A1_001428"
     CDS             16987..18255
                     /gene="dltD"
                     /locus_tag="ACL8A1_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HGS1202183.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            18410..18850
                     /gene="adcR"
                     /locus_tag="ACL8A1_001429"
     CDS             18410..18850
                     /gene="adcR"
                     /locus_tag="ACL8A1_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HGS1202184.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            18850..19554
                     /locus_tag="ACL8A1_001430"
     CDS             18850..19554
                     /locus_tag="ACL8A1_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HGS1202185.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYCVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            19547..20353
                     /locus_tag="ACL8A1_001431"
     CDS             19547..20353
                     /locus_tag="ACL8A1_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HGS1202186.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            20363..21868
                     /gene="adcA"
                     /locus_tag="ACL8A1_001432"
     CDS             20363..21868
                     /gene="adcA"
                     /locus_tag="ACL8A1_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HGS1202187.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNIAPVKAEHFHIFFGGTSQETLFEEMDNWPTYYPDNLSGQEIAQEMLAH"
BASE COUNT         6513 a         4224 c         4910 g         6560 t
ORIGIN      
        1 ccagaaaaac caaaaccaga taatagcaag ccacaagcag atgataagaa gccatcaact
       61 acaaataatt taagcaagga caagcaacct tctaaccaag cttcaacaaa cgaaaaagca
      121 acaaataaac cgaagaagtc attgccatca actggatcta tttcaaatct agcacttgaa
      181 attgcaggtc ttcttacctt ggcgggggca accattcttg ctaagaaaag aatgaaatag
      241 tgattgattc attcaaaaat gtttgtaaaa agtattcggc ttaaaagaaa agagtctggg
      301 ataaaaagat tttgatttta ggaattcttt attataaatt tttaaaatcg atagattaga
      361 caaaaaagcg aacaagacag aattctgagt gtcagataac tgattttgtt cgctttttat
      421 atttaatatt atacttttgt cctaggctct tttttgcatt atactttttc caattgcaag
      481 agggcttcaa tctctgccag ggtgctagct tgcgaaatgg ctccacggag tttggcagcg
      541 ccagatgttc cccggagata gtgaggagcg aggccgcgga attcacgaac tgcgacgttt
      601 tctcctttga ggttaatcaa tcgtttcaag tgttcgtagg cgatcttcat cttgtcttca
      661 aaggtcaaat caggtaggat ttctcctgtt tcaaagtaat ggttgatttg gttgaagagg
      721 taaggatttc ccatggcagc tcggccaatc atgactgcgt cagcaccaac ttcttcgatg
      781 cgttgcttgg cttcttggac agtacggata tcaccgttgg cgatgaatgg aatcttggtt
      841 agagcttggg caaccttgta aagggtctca aggtctgcgt ggccagtata catttgttca
      901 cgggtacggc catgcatggc gagggcagaa acacctgcag cctcagcagc gagggcattt
      961 tctactgcaa gagatgggtc cgcccagccg gtacgcattt tgacagtaag tgggatatca
     1021 aggacagact ggaccttgtt gatgatagag tagatcttgt caggatcctt gagccacata
     1081 gcaccagctt cgttcttcac gattttgttg acagggcagc ccatgttgat atcgacgata
     1141 tcggtcttgg tgttttcttg gatgaattct gctgcgcgtg ctaggctgtc ttcatcgcta
     1201 ccaaaaagtt ggatagagac agggttttcg ccctcatcga tatgaagcat gtgcagggtt
     1261 ttttcgttgt tgtattggat tcccttgtca gagaccattt ccattacaac gagtccagct
     1321 ccgagctctt ttgcgatggt acgaaaggct gagttggtca cgccagccat aggcgctaaa
     1381 acggtacgat tgggaatctc aacattgcca atcataaaag gtgtattaag atttgtcacg
     1441 aatgagttcc tccaggtcct tttcatcaaa gttgtaagta gtttggcaga attgacaagt
     1501 gatttctgcc ccgtggtctt cctctttcat ttcctgtaag tctgagcttg gaaggctggc
     1561 aagagcgttc ataaagcgtt catggctaca gtcacattgg aaacggattt cttcttcaga
     1621 aagacgcttg taggcttcgt ccccgtagat agccttgagg agggcttcga tatggtcgtc
     1681 gctttcgaga agagtagaga tagctggcat ttcttggatg cgtttttcaa agcgagcaat
     1741 ctcttctttc ttggctcctg gcaagacttg aactaggaaa ccacctgcaa ccttgacctt
     1801 gtcttcctcg tccaaaagga cattgaggcc gaccgctgaa ggcgtttgtt ggctttcagt
     1861 aaggtaaaag gcaaggtctt caccgatttc tccagagatg aggggagtta tagagttgta
     1921 aggatttcca gtaccgtagt ctgtgataac gaggaattga ccatttccaa caaaaggtcc
     1981 gactaggact tcaccagtcg cagtcttttt gatgtcaaca ccaggatttt gaacatagcc
     2041 tttgacgttc cccttggtat cagcgacggt gataatagca cctagagagc tagagcccaa
     2101 caccttaact gtaagtttgg tatttccttt ttcattggct gcgagaatct ggctggcgat
     2161 aagagttcga ccaagcgcta cagttgagct agcttgggtt tgatgttttt cttgagcagt
     2221 gcggacggtt tccgtgctat caaggacaaa agcacgaaag gctccgcttt ctgatatagt
     2281 tttaataatt ttatccatag ctactatttt agcataaaaa tgcccaaagg gggagccgtg
     2341 tgtttactga ttttcaggat aatggaccag gaaatcagca tgaaaataaa aagagaaaca
     2401 gattatttta gcatttgtaa gatttatgct atgcttaagg tagaaaatga aaggggtaac
     2461 aaatgtattt aggagatttg atggagaaag ccgagtgtgg tcaattttca atcctttcct
     2521 ttctattaca agagtctcag acgaccgtca aggctgtaat ggaagaaaca ggattttcaa
     2581 aagcaaccct aaccaaatat gtcaccctgc tcaatgacaa ggctttggat agtggcttag
     2641 aactgactat tcactcagaa gatgaaaatc tgcgtctgtc tatcggtgca gctaccaagg
     2701 ggagagatat tcggagcttg tttttggaga gtgctgttaa ataccagatt ttggtttatc
     2761 ttttctacca ccaacagttt ttagcccatc agctggctca agaattggtg attagcgagg
     2821 ctacgcttgg tcgtcacttg gctggtttaa atcagatttt gtcagaattt gatttatcca
     2881 tccaaaatgg acgttggcga ggtccagagc atcagattcg ctatttctat ttctgtcttt
     2941 tccgaaaggt ctggtcgagt caggaatggg aaggtcacat gcagaaacca gagagaaaac
     3001 aggagattgc caatttagag gaaatctgcg gtgcaagttt gtctgtgggg cagaaattgg
     3061 acttggttct ctgggctcac atcagtcaac aacgtcttcg ggtcaatgct tgtcagtttc
     3121 aagtcataga agagaaaatg cgagggtatt ttgacaatat cttctatctt cgtttgctga
     3181 gaaaggttcc gtcctttttt gctgggcaac atattccact aggagttgag gatggtgaga
     3241 tgatgatatt cttctctttt ctcctatctc atcgcattct tcctcttcat actatggagt
     3301 atattcttgg ttttggaggg cagttggcag atttactgac gcaattgatt caagaaatga
     3361 agaaggagga actattgggg gattatacag aggaccatgt cacctatgaa ctcagtcagc
     3421 tttgtgctca agtctatctc tataagggct atattttaca ggatcgctac aagtaccagt
     3481 tagagaatcg tcatccatat ttactgatgg aacatgattt taaagagaca gcagaggaga
     3541 tttttcatgc tctacctgct tttcaacagg ggacagattt agataagaag attctctggg
     3601 aatggctcca gttaatcgaa tatatggctg aaaacggtgg tcagcatatg cggattggtc
     3661 tggatttgac atctggtttt cttgtctttt caaggatggc agccattttg aaacggtatt
     3721 tggaatacaa tcgttttatt accattgaag cttatgaccc tagtcggcat tatgatttgc
     3781 tggttaccaa taacccgatt cataagaagg aacagacacc agtctattat ttaaaaaatg
     3841 acttggatat ggaggatttg gtagcgattc gccagttatt attcacttaa aaggcttggt
     3901 taatccaggt cttttttgtg aaattcacac aatctcctca cattttttta aaaattaaaa
     3961 aaagttgata aacaagaaag cgctttattt tgtatactag taagtgtaaa gaggaaacac
     4021 ctcaagatct ttatcaggag gacagtatat gtcacaagaa aaatacatca tggccattga
     4081 ccagggaact acaagttctc gtgccatcat tttcaacaaa aaaggggaaa aggttagctc
     4141 gagtcaaaaa gagtttactc agattttccc acaagcaggt tgggttgagc acaatgccaa
     4201 tgaaatttgg aactcagtac agtcagttat tgcgggtgct ttcatcgaaa gtggtgtcaa
     4261 gccaaatcaa atcgaggcaa tcgggattac taaccagcgt gaaacaacgg ttgtctggga
     4321 taagaaaaca ggacttccta tctacaatgc tatcgtttgg cagtcacgcc agacagcacc
     4381 tttggctgag caactaaaaa gccaaggtta tgtggaaaaa ttccatgaaa agactggttt
     4441 gattattgat gcttacttct ctgctaccaa ggttcgttgg attttggatc atgtagaagg
     4501 tgctcaagag cgagcagaaa aaggggaatt gctctttggt actatcgata cttggttggt
     4561 ttggaaattg actgacggtg cggctcacgt gactgactac tcaaatgcag ctcgtaccat
     4621 gctttataac attaaagaac tcaaatggga tgatgagatt ttggaaatcc ttaacattcc
     4681 gaaggctata cttccagaag ttcgttctaa ctccgaaatc tacggcaaga cagctccatt
     4741 ccatttctac ggtggagagg tgccaatctc aggtatggct ggggaccaac aagcagccct
     4801 ctttggacag ttggcttttg agccaggtat ggttaagaat acttatggaa caggctcttt
     4861 catcatcatg aatactgggg aagagatgca gttgtctgaa aacaacctct tgacaaccat
     4921 tggttacgga atcaacggta aggtttatta tgccttggaa ggttctatct tcatcgcagg
     4981 aagtgctatt cagtggcttc gtgacggtct tcgcatggtt gaaaattcac cagaatctga
     5041 aaaatacgct cgtgattctc acaacaacga tgaagtttat atcgttccag cctttacagg
     5101 tctaggcgct ccatactgga accaaaatgc tcgtggttcc gtctttggtt tgactcgtgg
     5161 aacaagcaaa gaagacttta tcaaggcgac tttgcaatct attgcttatc aagtgcgtga
     5221 catcatcgac accatgcaag tggatactca gaccgccatt caagtactga aggtggatgg
     5281 tggtgcagcc atgaacaact tcctcatgca gttccaggcg gatattttag gcattgatat
     5341 tgcacgtgct aaaaacttgg aaacaacagc tctaggagcg gccttcctag caggtttgtc
     5401 agtagggtac tggaaagact tggacgagtt gaaactcttg aacgagacag gagaactctt
     5461 tgagccatct atgaacgaat ctcgcaagga acaactctat aagggctgga agaaggctgt
     5521 gaaagcaact caagtctttg cggaaatcga tgactaatac tcaatgaaaa tcaaagagca
     5581 aactaggaag ctagctgcaa gctgtacttg agtacggtaa ggcgacgctg acgtggtttg
     5641 aagagatttt cgaagagtat aatactggca gaataaagcg atttagttag aaagtgtgta
     5701 aatatggaat tttcaaagaa aacacgtgaa ttgtcaatta aaaaaatgca ggaacgcacc
     5761 ctggacctct tgattatcgg tggaggaatc acaggagctg gtgtagcctt gcaggcggca
     5821 gctagcggtc ttgagactgg tttgattgaa atgcaagact ttgcagaagg aacatctagt
     5881 cgttcaacaa aattggttca cggaggactt cgttacctca aacaatttga cgtagaagtg
     5941 gtctcagata cggtttctga acgtgcagtg gttcaacaaa tcgctccaca cattccaaaa
     6001 ccagatccaa tgctcttacc agtttacgat gaagatggag caacctttag cctcttccgt
     6061 cttaaagtag ccatggactt gtacgacctc ttggcaggtg tgagcaacac accaactgcg
     6121 aacaaggttt tgagtaagga tcaagtcttg gaacgccagc caaacttgaa gaaggaaggc
     6181 ttggtaggag gtggagtgta tcttgacttc cgtaacaacg atgcgcgtct cgtgattgaa
     6241 aacatcaaac gtgccaacca agacggtgcc ctcattgcca accacgtgaa ggcagaaggc
     6301 ttcctctttg acgaaagtgg caagattaca ggtgttgtag ctcgtgatct cttgacagac
     6361 caagtatttg aaatcaaggc ccgtctggtt attaatacaa caggtccttg gagtgataaa
     6421 gtacgtaatt tgtctaataa gggaacgcaa ttctcacaaa tgcgcccaac taagggagtt
     6481 cacttggtag tggattcaag caaaatcaag gtttcacagc cagtttactt tgacacaggt
     6541 ttgggtgacg gtcgtatggt ctttgttctc ccacgtgaaa acaagactta ctttggtaca
     6601 actgatacag actacacagg tgatttggag catccaaaag taactcaaga agatgtagat
     6661 tatctacttg gcattgtcaa caaccgcttt ccagaatcca acatcaccat tgatgatatc
     6721 gaaagcagct gggcaggtct tcgtccattg attgcaggga acagtgcctc tgactataat
     6781 ggtggaaata acggtaccat cagtgatgaa agctttgaca acttgattgc gactgttgaa
     6841 tcttatctct ccaaagaaaa aacacgtgaa gatgttgagt ctgctgtcag caagcttgaa
     6901 agtagcacat ctgagaaata tttggatcca tctgcagttt ctcgtgggtc tagcttggac
     6961 cgtgatgaca atggtctttt gactcttgct ggtggtaaaa tcacagacta ccgtaagatg
     7021 gctgaaggag ctatggagcg cgtggttgac atcctcaaag cagaatttga ccgtagcttt
     7081 aaattgatca attctaaaac ttaccctgtt tcaggtggag aattgaaccc agcaaatgtg
     7141 gattcagaaa tcgaagcctt tgcgcaactt ggagtttcac gtggtttgga tagcaaggaa
     7201 gctcattacc tagcaaatct ttacggttca aatgcaccga aggtctttgc acttgctcac
     7261 agcttggaac aagcgccagg tcttagcttg gtagatactt tgtcccttca ctatgcaatg
     7321 cgcaacgagt tggctcttag cccagttgac ttccttcttc gtcgtaccaa ccatatgctc
     7381 tttatgcgtg atagcttgga tagcatcgtt gagccagttt tggatgaaat gggacgattc
     7441 tatgactgga cagaagaaga aaaagcaact taccgtgctg atgtcgaagc agctctcgct
     7501 aacaacgatt tagcagaatt aaaaaattaa gaaaaaataa aagaggtgga gggcagcatt
     7561 ccttgtcgcc cgtcccttct ttttaatgga gacagaaaga tgatgaatga attatttgga
     7621 gaatttctag ggactttaat cctgattctt ctaggaaatg gtgttgttgc aggtgtggtt
     7681 cttcctaaaa ccaagagcaa tagctcaggt tggattgtga ttactatggg ttgggggatt
     7741 gcagttgcgg ttgcagtctt tgtatctggc aagctcagtc cagctcattt aaacccagct
     7801 gtgaccatcg gtgtggcctt aaaaggtggt ttgccttggg cttccgtttt gccttatatc
     7861 ttagcccagt tcgcaggggc catgctgggt cagattttgg tttggttgca attcaaacct
     7921 cactatgagg cagaagaaaa tgcaggcaat atcctggcaa ccttcagtac tggaccagcc
     7981 atcaaggata ctgtatcaaa cttgattagc gaaatccttg gaacctttgt tttggtgttg
     8041 acaatctttg ctttgggtct ttacgatttt caggcaggta tcggaacctt tgcagtggga
     8101 actttgattg tcggtatcgg tctatcacta ggtgggacaa caggttatgc cttgaaccca
     8161 gctcgtgacc ttggacctcg tatcatgcac agcatcttgc caattccaaa caagggagac
     8221 ggagactggt cttacgcttg gattcctgtt gtaggccctg ttatcggagc agccttggca
     8281 gtgcttgtat tctcactttt ctagtttata ctcttcgaaa atcaaattca aaccacgtca
     8341 gcgtcgcctt accgtactca agtacagctt gcggctagct tcctagtttg ctctttgatt
     8401 ttcattgagt attagaaaac aattatgttg atagagcttg ggcaagagcc caatttcagc
     8461 aaaaaatgaa gtaaatcttc tcataataaa acgcatcata tcaagcacga aaattccacg
     8521 aggtcaacta cagtcagaaa gctgaacaac aagccaaaac gcccaaaaaa ggcggcaaaa
     8581 agcaagcacc tgcaagcaac gtgccgaaat ggtcaaatcc tgattatgtc aacgaattag
     8641 acccaaaaat cgttgatatg ctagtagaat ttcacaagtc acaaggcact ttggaaactc
     8701 ccgaggcgca agcagaaatc gcccaaaaac gtgaagaaat cgagcaaagg agagctgagc
     8761 ttgaggataa aaaacaagag cttttgaacc gcttgaacaa atagagtttc gcaagtatta
     8821 tgcttacaaa ttacttgagc aattaactaa aatataaacc ctgcctttat atctaggcag
     8881 ggtttatatt ttagaaattc acgtaggttg ttacggtttt tacataccca gtatagtttg
     8941 agtttctata gtattcagtg ataaacttcc attttctttg agcaacatgg atataagtac
     9001 ttgttatgta gtatggatat gggctttgtg aatccaagta agactgataa gcttgtatac
     9061 caaaatatgc tccaccaatt attgcacccc atggaccccc caataaagca cctatcctac
     9121 caatcatata actgattcca gcaccagtca tgaagttagc gaatgtgtta gcttgtttat
     9181 tcccatgtat tgtgttgacg taattccaaa cattaggatc gtatgatcta aaagatatat
     9241 ttaggtcgat ttcattcttt tgataagcca tataaaatgc cccattgata tagacgccgt
     9301 cagcacgtcg ttcaatagtg tctacacttc catctggatt gacaacctca agaacttcat
     9361 cgcttaaaat atttacttgc gtatctccga accgcactga tgagccattc tcaaactgag
     9421 cctcaccaga tacaacttta gagtttgccg ataagctatc atcagcaaaa acaaacaagc
     9481 gacggggaaa tgctagacat acagaaaaca gacataacta gcaaacacat gcatttaaac
     9541 atcttagaca taacggaaac tcctttgtat ttttgatttt tttcaacttt tattatacaa
     9601 taaaaccaaa taaaaagaaa gcggtaacaa tatgcttaat gcgaaaattt tttatatatt
     9661 tttatgtttg atcgttatcg aaactacagg cttgttgttg ttgaaaagag gtctcgaaat
     9721 gggttattta gacacagaag ctattatcct cgcagttttt tcatttgctt tttacaacct
     9781 atgttcattc gcttgggtct gctctacaat aaaaaaacaa taaaaaataa atagacgtat
     9841 tttcaaaaaa aaaaaaaatg catatttata ttagcaaaac gacgatttaa atcgtcgttt
     9901 ttttgtagta cgacgggcat gtcgtatatc tgaggtgtaa gtcctcggtg ggcacctgct
     9961 accggtgaac ccaatagcga ttcccaagcc tgactatcgt gaggtagcag ggagaggaag
    10021 ggatagcgaa atcgtggctc tacgaacagg aacgtgatag taaggcgtat atagcggata
    10081 aggaggcttc aaactctaaa gtccaaaaag gtagtcgtaa cctatatgtg taaatcacga
    10141 gagtaattga attcggacta aggtttgtgt gaaaaagata aatctttcta gagtctaaag
    10201 actctgcgtc agatttccta ttttcactgt aaccttttaa cgtcctcata tcttgtataa
    10261 acgaggaaag atgtacgact tatcccgtga ggtttcatga gcgctgaaag cgtagtaaca
    10321 acgaatcatg agaagtcagc cgaacccata gtagtgagga aacttctgta gtggaagtgg
    10381 agcgaagggg tgaatactca aacagtctgg ggagagactg tttgaggtct gtcgctagaa
    10441 agagaaaacg acagatcgaa gtaatcctac ttcacttgtg tctgtaaaat gagtggtctg
    10501 atagaactgg actttgacac gtaatgacta gatgaaggtt caccaatata agatgtccct
    10561 caggcaccaa aacaagaaag gaatacgcac atgtcaaaac tgctagataa gatattatca
    10621 cgcgaaaata tgctggaagc ctacaatcaa gtaaaatcca ataaaggctc agctgggatt
    10681 gatggaatga ctatcgaaga gatggataat tatctcagac aaaactggcg cttgactaag
    10741 gaactgataa aacagagaaa atataagcct caaccagttc ttaaagttga gatacctaaa
    10801 ccagacggag gcatccgtca actaggaatt ccaacagtta tggatagaat gattcaacag
    10861 gccattgtcc aagtcatgag ccccatttgt gaaccccatt tctcagatac gagttatggt
    10921 ttcagaccaa ataggtcatg tgaaaaagcc atcatgaagc tcttagaata cttaaatgac
    10981 ggctatgagt ggatagtgga tatagaccta gagaaatttt tcgatacagt tcctcaagat
    11041 agattgatgt ccttagtaca taacattatc gaagacggag atacggaatc cttgattcgt
    11101 aagtatcttc attcaggtgt tatcattaat ggtcaacgtt ataaaacact agttggtaca
    11161 ccacagggag gaaatttatc tcctctctta tccaatatca tgcttaatga attggacaag
    11221 gaattagaaa agaggggact tcgatttgtg cgctacgcag atgattgtgt gattacggtc
    11281 ggaagcgagg cagcctctaa gcgtgtgatg tattcagtca gtcgttttat tgagaaacgg
    11341 ctaggtttga aagtaaacat gaccaagact aagattacca gaccaagaga gttgaaatat
    11401 ctaggttttg ggttctggaa atcatcagat ggttggaaaa gccgtccaca tcaagatagt
    11461 gttcggagat ttaagtttaa attgaagaaa ctaacacaga ggaaatggag tattgagcaa
    11521 ctgaatttgt ctattcgagg atggataaac tatttctcat tgggaaatat gaaaagtata
    11581 gtcgccagca tagatgagcg cttgcgtact cgcctacgag tgattatctg gaagcaatgg
    11641 aagaagaaat cgagacgatt atggggattg cttaagttag gagttcctaa atggatagca
    11701 gataaggtat ctggctgggg cgaccattat caattagtag ctcagaagtc ggtacttaaa
    11761 cgtgctatat caaaaccagt cctggaaaaa cgtggactgg tttcgtgttt ggattattac
    11821 cttgaacgac atgcgttaaa agttagttga accgccgtat gccgaacggc acgtacggtg
    11881 gtgtgagagg ggctagagat tatcccctac tcgattaact cccctgaaat ttattttaat
    11941 tatgcaaatt tcacgtattt ttgatgctga gacgacgatc ctgggaactt ttcagatatt
    12001 tttttgacta tctaaatcta tcattagaaa agcttagagc gccaaaggat ttgagcgttt
    12061 ttctgatttt taagactttt tccagtctct ttttcgattg aagatgtaat tattctacta
    12121 actaactaac ttcttagtac tagccaacaa cgataatcat aattcctcct aaaattagga
    12181 ataataaagg caatagtttt tgttttttca tgtaaaaaac ctcacttttg ttttctgcta
    12241 ttttatgcta aaatattaaa aatcaaattt aattccaaag tttgtaacta aagggggagc
    12301 gctacatgtc taatttattt gtcaagttgt tagtctctca attatttgca aatttagcag
    12361 atattttctt tagagtaaca atcattgcta acatatacat tatttcaaaa tcagtaattg
    12421 ccacatcact agttcctatc ttaataggaa tatcctcttt tgttgcgagt cttttagttc
    12481 cgttggttac taaaaggtta gcgctaaata gggttttatc tttatctcaa tttggaaaga
    12541 ctatattatt ggcgatactg gtaggaatgt ttaccgtaat gcaatccgta gcgcctttgg
    12601 tgacctatct atttgttgtt gcaatttcca tactagatgg ttttacagca cccgtttcct
    12661 atgctattgt gccacgctat gcgaccgatt tgggtaaggc taattcagcc ttatcaatga
    12721 ctggtgaagc tgttcaattg ataggttggg gattaggtgg actcttgttt gcaacaattg
    12781 gtctgttacc taccacgttt atcattttag tcttgtatat catttctagc tttctgatgt
    12841 tatttcttcc taacgctgaa gtggaggtgt tagagtcaga aactaatctt gaaattttgc
    12901 tcaaaggttg gaagttagtt gctagaaatc ctagattaag actttttgta tcagcaaatt
    12961 tattggaaat ttcttcaaat acgatttggg tttcttccat tatacttgtt tttgtaacgg
    13021 agttattaaa taaaacggaa agttactggg gatattctaa tacagcatac tctattggta
    13081 ttataattag tggcttaatt gcttttaggc tatctgaaaa gttccttgct gctaaatggg
    13141 aaagtattct ttttcctctt gtagctatgg caatagtgac actgactatt ctatattttc
    13201 caaacgcaca gatgttttta ttattttcag ctttagttgg tatgttatcg caactaaaag
    13261 aagttcctga aagtgtattt cttcaggaaa cagtagagga aaatcattta gttaatgtct
    13321 attccgttct tgaagtgatt tctacattag cattttcagt atttgttttg ctaatgagct
    13381 atattactga gagttttggt attagcatca gtttttggct atcagccatt tgtctgatga
    13441 tagaagctat tttaatttat atcagacgag attattttaa atgagagagt ggttaaaaag
    13501 tctttaaaaa tcagaaaagc gcatagtatc aggtgttgaa aaaacttgat attgtgtgtt
    13561 ttattatggg aagatttact tcattgtctc ctgaaatgga gttttgaagc aggatcactc
    13621 tatttgaaaa tatacataga aaaactgtat aatccttaaa ataagctgaa atctattgga
    13681 aacatacggt aaacaattga aaagatacta tataaagtgg tacaatggta gaaaaataag
    13741 ctagtaagaa tcactcttat tttagcgaaa aattacagaa atgaatggtt tttcttgatg
    13801 aaacagagaa aagaattgta cctctttctt ggtcggacag ccttgtattt tcttatcttt
    13861 ctagggctgc tttacttctt tagctatctt ggtcagggtc aaggaagctt tatctataat
    13921 gaattttaac ttgaaggaga atccctattg tgtcaaataa accaatagca gatatgattg
    13981 aaaccattga gcattttgct cagacacagc ctagctatcc tgtctataat gttttggggc
    14041 aggaacacac ttatggcgat ttaaaggctg attcggatag tttggctgca gtcattgacc
    14101 aactaggctt gcctgagaag tctcctgtgg ttgtttttgg tggccaagaa tatgaaatgt
    14161 tggcaacctt tgtagcgctg actaagtcag gtcatgccta cattccaatt gatagccatt
    14221 cggccttgga gcgagtttca gctattttag aagtagcaga gccaagcttg attattgcca
    14281 tttcagcctt tcccttggag caggtttcta caccaatgat aaatctagct caggttcaag
    14341 aagcctttgc ccaagggaac aactatgaaa tcacgcatcc agtcaaggga gatgataatt
    14401 actacattat ctttacttct ggtacgactg gtaagcctaa gggagtgcag atttcacatg
    14461 ataatctcct cagctttaca aactggatga ttacggataa ggaatttgcg acaccgagtc
    14521 gtccgcaaat gctggcacag ccaccttatt cttttgactt gtctgtcatg tattgggcac
    14581 cgaccttggc acttggtggt acgcttttca ctcttccttc agtcatcact caggacttta
    14641 agcaactctt tgcggctatc ttttcattgc caatcgctat ctggacatca acaccatcct
    14701 ttgcagatat ggccatgttg tctgaatact tcaacagtga gaaaatgcct ggaatcacgc
    14761 atttctactt tgatggtgaa gaattgacgg tcaaaacagc tcaaaaactg cgcgagcgtt
    14821 tcccaaatgc ccgtatcatc aatgcttacg gcccaacaga agcgacagta gctttgtcag
    14881 cagttgccgt gacagacgag atgttagcga ctctcaaacg cctaccaatc ggctatacca
    14941 aggctgattc tccaaccttt atcattgacg aggaaggaaa taaactgcca aatggtgagc
    15001 agggagaaat cattgtttct gggccagctg tttcaaaagg ttatatgaaa aatcctgaaa
    15061 aaacagcaga agccttcttt gagtttgaag gtctgccagc ctatcacaca ggcgatgtgg
    15121 gaaccatgac agatgagggc ttgcttctct acggcggacg catggacttc cagattaagt
    15181 ttaacggtta ccgcattgag ttagaagatg tctctcaaaa cctcaacaag tctcgcttta
    15241 tcgaatctgc tgtcgcagta ccgcgctata acaaggacca caaggtacaa aatctattgg
    15301 cttatgtcat cttaaaagac ggtgttcgtg agcagtttga gcgagatatc gatattacca
    15361 aggccatcaa ggaagacctg acagacatca tgatgtccta tatgatgcca tctaaattcc
    15421 tttaccgaga cagtttgcca ctaactccaa atggaaagat tgacatcaaa ggattgatta
    15481 acgaggtgaa taagagatga tggagttttt tcaacagctt cctcatttag agccatatgg
    15541 taatcctcag tattttgttt atgtgattgc tgcaaccttg cccatcttta taggtctctt
    15601 tttcaagaaa cgctttgcct ggtatgaagt gttggtaagt ctcttcttta ttgtcaccat
    15661 gttggtgggt ggaaagacca atcaactagc tgccttgggt atttaccttt gctgggaaat
    15721 attgctcctg cttttctaca agcattatcg aaaaagcaag gatggcaagt gggtcttcta
    15781 cttagttagt tttctgtccc tacttccgat tatctttgtc aaggtgcaac cagctatcaa
    15841 tggaacgcag tctttgcttg ggttcttggg aatttcttac ctgacctttc gttcggttgg
    15901 gattatcatc gagctgagag atggagtgat taaggatttt accctctggg aattcctccg
    15961 tttccttctc ttcatgccaa ctttctcgag tggtccaatc gatcgcttta agcgatttaa
    16021 tgaaaattat caggctattc ctgagcgaga tgagttgatg gatatgctgg atgaatctgt
    16081 ccgctatatc atgcggggct ttttgtataa gtttatccta gctcatgttt taggagagac
    16141 cttactacct cctctgaaga atttagcctt gcagtcaggt ggcttcttta atctctatgc
    16201 cttggcagtt atgtatactt ttggtctgga gctcttcttt gactttgcag gttattctat
    16261 gtttgctttg gccatctcaa acttgatggg aatccgtagc cctatcaact ttaacaagcc
    16321 ctttttatca agggatttaa aggagttttg gaatcgctgg catatgagtc tgtccttctg
    16381 gttccgtgac tttgtcttta tgcgaatggt gatggtgtta accagaaaga aagtctttaa
    16441 aaatcgtaat gtaacctcaa gcatggccta cattgtaaat atgctgatta tgggattttg
    16501 gcatggcgtg acctggtact atatcgccta tggactcttt catggactag gcttggtcat
    16561 caatgatgcc tgggttcgca agaaaaaaac gctcaataag gaacggaaaa aagcagggaa
    16621 ggctgcccta cctgagaatc gctggattca gttgcttggc atggttgtca ctttccatgt
    16681 tgtcatgttg tcattcttaa tcttttctgg attcttgaat aatctatggt ttaaaaaata
    16741 aaaggaaata aaatatggat atcaaatcag aagttattga aattattgat gagttgttta
    16801 tggaagatgt ttctgacatg atggatgaag atctttttga tgcaggtgtc ttggatagta
    16861 tgggaacggt tgagttgatt gtggagattg agaaccgttt tgacattcgt gtccctgtaa
    16921 cagagtttgg tcgtgacgac tggaatacag ctaataaaat catagctggt attgtggagc
    16981 tacaaaatgc ttaaacgctt atggatgatc ttcggaccgg tcttgatcgc tggtttgttg
    17041 gtttttctgc tcattttctt ttatcctact gagatgcatc ataatctagg agctgaaaag
    17101 cgttcagcag tggctactac tatcgatagt tttaaggagc gaagtcaaaa agtcagagca
    17161 ctatctgatc caaatgtgcg ttttgttccc ttctttggct ctagtgaatg gcttcgtttt
    17221 gacggtgctc atcctgcggt attagctgag aaatacaatc gttcctaccg tccttatctt
    17281 ttaggacagg ggggagctgc atcgcttaac caatattttg gaatgcaaca gatgttacca
    17341 cagctggaga ataaacaagt tgtgtatgtt atctcacctc agtggttcag taaaaatggc
    17401 tatgatccag cagccttcca gcagtatttt aatggagacc agttgactag ttttctgaaa
    17461 catcaatctg gggatcaggc tagtcaatat gcagcgactc gcttactgca acagttccca
    17521 aacgtagcta tgaaggacct ggttcagaag ttggcaagta aagaagaatt gtcgacagca
    17581 gacaatgaaa tgattgaatt attggctcgt tttaatgaac gccaagcctc cttttttggt
    17641 cagttttcgg ttagaggcta tgttaactac gataagcatg tagctaagta tttaaaaatc
    17701 ttgccagacc agttttctta tcaagcaata gaagatgttg tcaaagcaga tgctgaaaaa
    17761 aatacttcca ataatgagat gggaatggaa aattatttct ataatgagca gatcaagaag
    17821 gatttgaaga aattaaagga ttctcagaaa agctttacct atctcaagtc gccagagtat
    17881 aatgacttgc agttggtttt aacacagttt tctaaatcta aggtaaaccc gatttttatc
    17941 attccacctg ttaataaaaa atggatggac tatgctggtc tacgagagga tatgtaccaa
    18001 caaacggtgc agaagattcg ctaccagtta gaaagtcaag gttttaccaa tatagcagat
    18061 ttttctaagg acggcgggga gcctttcttt atgaaggaca ccattcacct tggttggttg
    18121 ggttggttgg cttttgacaa ggcagttgat cctttcctat ccaatcccac accagctccg
    18181 acttaccatc tgaatgagcg ctttttcagc aaagattggg cgacttatga tggagatgtc
    18241 aaagaatttc aatagataat ataatataga agagttcaag aatgaaacta gttttcacgt
    18301 tttttcttga ctcttttttt gcttgtgata taattaactg gtaaacaaaa tgtcaaagaa
    18361 tagtattttt ctcttaaaaa agagaagtct aaaaggaaag gagtcagata tgagacagct
    18421 agcaaaggat atcaatgctt ttttgaatga ggtgattttg caggcggaaa atcagcatga
    18481 aatcctaata ggtcattgca ctagcgaggt ggccctgacc aatactcagg agcatatcct
    18541 tatgctcttg tcagaggaat ctttaacaaa ttcagaattg gcccgtcgtc tcaatgtcag
    18601 tcaggcggca gttaccaagg ccattaagtc tttggtcaag gaagggatgt tggaaacatc
    18661 taaagattct aaagatgcgc gtgtgatttt ttatcagttg actgacttgg ctcgtccaat
    18721 cgctgaggag caccaccatc accatgagca tacactttta acctatgaac aagtggcgac
    18781 tcagtttact ccaaatgaac aaaaagtgat tcagcggttt ttgactgctt tagtaggaga
    18841 aatcaaataa tgagatatat tacggtagag gatttgtcct tctattatga taaggagcct
    18901 gttcttgaac atatcaatta ttgtgttgat agtggggaat ttgttacctt gactggggaa
    18961 aatggagcgg ctaagacgac gctcatcaag gctagtcttg gaattctgca accacgcatt
    19021 ggaaaggtgg ctatttcaaa gacaaatacg caaggtaaga aattgagaat agcctatctt
    19081 cctcaacaaa ttgccagttt taatgctggt tttccaagta cggtctatga atttgtcaag
    19141 tcgggtcgct atccgagaaa aggctggttc cgtcgtttga atgctcatga tgaggagcat
    19201 atcaaggcta gtctggactc agttggcatg tgggaacatc gagacaaacg cttggggtct
    19261 ctatctgggg gacaaaagca gcgagcggtg attgcgcgta tgtttgcttc tgaccctgat
    19321 gtgtttatcc tagacgagcc gacaacgggg atggatgcag gaagtaaaaa tgaattttac
    19381 gaactcatgc accacagcgc ccatcatcat ggcaaggctg ttttgatgat tacccatgac
    19441 cctgaagaag ttaaggatta tgcggatcgc aatattcatc tagtccgtaa ccaagactcg
    19501 ccatggcgtt gtttcaacgt tcatgagaat ggccaggagg tgggtcatgc ttagtttatt
    19561 atcttacgac tttatacaac gcgccttttt ggcggttatt gctatgagtc ttttctcacc
    19621 ggtattggga accttcctca tcttgcgtcg tcagagtttg atgagtgata cccttagcca
    19681 cgtctcactt tcaggtgtag cctttggtct ggttttgggg atttctccaa ctgtttctac
    19741 tattgccatt gtcttgattg cggcggtctt tctggagtat ctccgtacgg tttacaagag
    19801 ctttatggaa atcgggacag ctatcctcat gtcaacaggt ctggctgttt ctctgattgt
    19861 catgagcaag ggtaaaagct cgagttcaat gagtttggac caatatctct ttggttcgat
    19921 cgtgactatc agtgaagaac aggtcatttc cctctttgtc attgcggcgg ttgttttgat
    19981 tttgaccttt ctctttcttc gtcctatgta tatcttaact tttgacgaag atacggcctt
    20041 tgtggatggc ttgccagttc gtaccatgtc cattcttttt aacatggtga caggggtggc
    20101 tattgccctt atgattcctg cagcaggagc tcttctggta tcgaccatta tggtcttgcc
    20161 agctagtatt gccctgcgtc tggggaaaaa ctttaaatcg gttatgctgc ttgccagtgc
    20221 gattggcttt ttgggaatgg tagcaggact ttatatttcc tactatgcag aaacacctgc
    20281 aagtgcaagt attaccatta tttttgtaac tgtctttata ctaatcagtt tagtaagacg
    20341 ttttatcaaa taggagacga acgtgaaaaa aattagctta ttactagcca gtctatgtgc
    20401 cttgttttta gtggcttgtt ccaatcaaaa acaggcagat ggtaaactca atatcgtgac
    20461 aaccttttac cctgtctatg aatttaccaa gcaagtcgca ggagatacgg ctaatgtaga
    20521 actcctaatc ggtgctggga cagaacctca tgaatacgaa ccatctgcca aggcagttgc
    20581 caaaatccaa gatgcagata ccttcgttta tgaaaatgaa aacatggaaa catgggtacc
    20641 taaattgcta gataccttgg ataagaaaaa agtgaaaacc atcaaggcga caggcgatat
    20701 gttgctcttg ccaggtggcg aggaagaaga gggagaccat gaccatggag aagaaggtca
    20761 tcaccatgag tttgaccccc atgtttggtt atcaccagtt cgtgccatta aactagtaga
    20821 gcacatccgc gacagcttgt cagcagatta tcctgataaa aaagagacct ttgagaagaa
    20881 tgcagctgcc tatatcgaaa aattgcaagc cttggataag gcttacgcag aaggcttgtc
    20941 tcaagcaaaa caaaagagct ttgtgactca acacgcagcc tttaactatc ttgccttgga
    21001 ctatggactc aaacaagtcg caatctcagg actttctcca gatgcagagc catcagctgc
    21061 tcgcttggca gaattgacag agtatgtcaa gaaaaataaa atcgcctata tctattttga
    21121 agaaaatgcc tcacaagccc ttgctaacac actttcaaaa gaagcaggtg tcaaaactga
    21181 tgtcctcaat cctttagaaa gtctgacaga agaggacacc aaggctggag aaaactacat
    21241 ttccgtgatg gagaaaaacc tcaaggcttt gaaacaaaca acagaccaag aaggcccagc
    21301 aatcgaacct gaaaaggcag aggataccaa gacagtccaa aatggttact tcgaggatgc
    21361 agctgtcaag gaccgcacct tgagtgacta tgcaggtaac tggcaatcag tttatccttt
    21421 ccttgaagac ggcacgtttg accaagtctt tgactacaag gctaagttga ctggtaagat
    21481 gacccaggct gagtacaagg cttactatac aaaaggctat cagacagatg tgactaagat
    21541 taacattact gataatacta tggaatttgt tcaaggtgga caaagcaaga aatacactta
    21601 caagtatgtc ggtaagaaaa ttttgactta caagaaaggc aatcgtggcg tgcgtttcct
    21661 ctttgaagcc acagatgctg acgctggaca attcaagtat gttcagttta gtgaccacaa
    21721 tatcgcccca gttaaggcag aacatttcca tatctttttt ggaggtacaa gccaagaaac
    21781 tctatttgaa gaaatggata attggccaac ctactaccca gataacctat ctggccaaga
    21841 aatcgcccaa gaaatgttgg cgcattgatg agaaatcgac tagttcataa gagctagtcg
    21901 gttttttgat actagatggc taacttcatt atagaccttt cagaactaag ggaacctgaa
    21961 aaaataaata aaactcttga aatatctggg cttatatgct agaatgtagt tagtgtataa
    22021 ggttcattag aacaccaaag cccctaacta gtgccatagt taagggcttt tttgacgtat
    22081 cttagcgatt taacgggtgt ttataggcta gtcattcggt ctacttctta ccatctagcc
    22141 atttgcagac gaaatacaag ataattccag ctataacatc tgctataata gtaagagcaa
    22201 gctctgg
//
LOCUS       DBERMR010000035        21885 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.035, whole genome shotgun sequence.
ACCESSION   DBERMR010000035 DBERMR010000000
VERSION     DBERMR010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 21885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21885
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.035"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     misc_feature    80..211
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            227..757
                     /gene="infC"
                     /locus_tag="ACL8A1_001433"
     CDS             227..757
                     /gene="infC"
                     /locus_tag="ACL8A1_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HGS1202188.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            790..990
                     /gene="rpmI"
                     /locus_tag="ACL8A1_001434"
     CDS             790..990
                     /gene="rpmI"
                     /locus_tag="ACL8A1_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HGS1202189.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            1042..1401
                     /gene="rplT"
                     /locus_tag="ACL8A1_001435"
     CDS             1042..1401
                     /gene="rplT"
                     /locus_tag="ACL8A1_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HGS1202190.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            1459..1839
                     /locus_tag="ACL8A1_001436"
     CDS             1459..1839
                     /locus_tag="ACL8A1_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGS1202191.1"
                     /translation="MVSKMLHTCLRVENLEKSIAFYKDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            2089..2889
                     /locus_tag="ACL8A1_001437"
     CDS             2089..2889
                     /locus_tag="ACL8A1_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HGS1202192.1"
                     /translation="MNLTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            2852..3838
                     /locus_tag="ACL8A1_001438"
     CDS             2852..3838
                     /locus_tag="ACL8A1_001438"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HGS1202193.1"
                     /translation="MVLFSAQEQLYYKEKIMTTNRLQVSLPGLDLKNPIIPASGCFGF
                     GQEYAKYYDLNLLGSIMIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAE
                     KLPWLEREYPNLPIIANVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGL
                     LIGQDPDLAYDVVKAAVEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVG
                     MRFDLKTRKPILANGTGGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEM
                     YLAGASAIGVGTANFTNPYACPDIIENLPKVMDKYGISSLEELRQEVKEFLR"
     gene            3880..5598
                     /locus_tag="ACL8A1_001439"
     CDS             3880..5598
                     /locus_tag="ACL8A1_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HGS1202194.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGTMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYMTANEWIWDKESWFYLKSDGKIAEKEWVYDSHSQ
                     AWYYFKSGGYMTANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYMAKN
                     ETVDGYQLGSDGKWLGGKATNKNAAYYQVVPVTANVYDSDGEKLSYISQGSVVWLDKD
                     RKSDDKRLAITISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIPVAS
                     HLSDMEVGKKYYSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINNSLL
                     ENKGATFKEAEEHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYLSAK
                     TFDDVDKGILGATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMKINE
                     KLGGKD"
     gene            complement(5659..7314)
                     /gene="pavA"
                     /locus_tag="ACL8A1_001440"
     CDS             complement(5659..7314)
                     /gene="pavA"
                     /locus_tag="ACL8A1_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HGS1202195.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKIIEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTKTSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            7419..7916
                     /gene="ybeY"
                     /locus_tag="ACL8A1_001441"
     CDS             7419..7916
                     /gene="ybeY"
                     /locus_tag="ACL8A1_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HGS1202196.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            7897..8292
                     /locus_tag="ACL8A1_001442"
     CDS             7897..8292
                     /locus_tag="ACL8A1_001442"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HGS1202197.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFALTGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLNIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            8309..9208
                     /gene="era"
                     /locus_tag="ACL8A1_001443"
     CDS             8309..9208
                     /gene="era"
                     /locus_tag="ACL8A1_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HGS1202198.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            9257..10081
                     /gene="mutM"
                     /locus_tag="ACL8A1_001444"
     CDS             9257..10081
                     /gene="mutM"
                     /locus_tag="ACL8A1_001444"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HGS1202199.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQAALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            10081..10686
                     /gene="coaE"
                     /locus_tag="ACL8A1_001445"
     CDS             10081..10686
                     /gene="coaE"
                     /locus_tag="ACL8A1_001445"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HGS1202200.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKPGG
                     RLFEALVQHFWQEIILENGELNRPLLASLIFSNPDEREWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            10673..11872
                     /locus_tag="ACL8A1_001446"
     CDS             10673..11872
                     /locus_tag="ACL8A1_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HGS1202201.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSEQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            11872..12021
                     /gene="rpmG"
                     /locus_tag="ACL8A1_001447"
     CDS             11872..12021
                     /gene="rpmG"
                     /locus_tag="ACL8A1_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HGS1202202.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHIDKIEVLKYCPKERKVTL
                     HLESK"
     gene            12061..12294
                     /gene="secG"
                     /locus_tag="ACL8A1_001448"
     CDS             12061..12294
                     /gene="secG"
                     /locus_tag="ACL8A1_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HGS1202203.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            12396..14750
                     /gene="rnr"
                     /locus_tag="ACL8A1_001449"
     CDS             12396..14750
                     /gene="rnr"
                     /locus_tag="ACL8A1_001449"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HGS1202204.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEAKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLELRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKKYHK
                     IVPSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGESYADVLSMMLLRSMQQARYSEHNYGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            14713..15180
                     /gene="smpB"
                     /locus_tag="ACL8A1_001450"
     CDS             14713..15180
                     /gene="smpB"
                     /locus_tag="ACL8A1_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HGS1202205.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKRQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            15196..16056
                     /gene="tehB"
                     /locus_tag="ACL8A1_001451"
     CDS             15196..16056
                     /gene="tehB"
                     /locus_tag="ACL8A1_001451"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HGS1202206.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMETVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            16129..17082
                     /gene="coiA"
                     /locus_tag="ACL8A1_001452"
     CDS             16129..17082
                     /gene="coiA"
                     /locus_tag="ACL8A1_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HGS1202207.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            17101..18903
                     /gene="pepF"
                     /locus_tag="ACL8A1_001453"
     CDS             17101..18903
                     /gene="pepF"
                     /locus_tag="ACL8A1_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HGS1202208.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQMEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYDPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLKGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALVVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHLYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            18905..19618
                     /locus_tag="ACL8A1_001454"
     CDS             18905..19618
                     /locus_tag="ACL8A1_001454"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HGS1202209.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGSLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            19685..20626
                     /gene="prsA"
                     /locus_tag="ACL8A1_001455"
     CDS             19685..20626
                     /gene="prsA"
                     /locus_tag="ACL8A1_001455"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HGS1202210.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIVG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(20745..21885)
                     /locus_tag="ACL8A1_001456"
                     /pseudo
     CDS             complement(20745..21885)
                     /locus_tag="ACL8A1_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         6586 a         4064 c         4879 g         6356 t
ORIGIN      
        1 caaaatacaa acacaaactt ttcaaaaaaa taacttttta tcttgacaag agctagaaaa
       61 cttggtatca tttaaaagtt gagaaaagca gaagtgagag cttctcgcct tgtgacatta
      121 agttgcctgg ccctacggat gaaaagtttc gaagaaacgc tatcataacg tgcgggcttg
      181 tatatttacg agtccgctat tgtttttctc taataaaaca aaagaggtga aaaccatagc
      241 aaagcaagac ttattcatca atgatgagat tcgtgtacgt gaagttcgct tgattggtct
      301 tgagggagaa cagctaggca tcaagccact cagtgaagcg caagctttgg ctgataacgc
      361 taatgttgac ctagtattga ttcaacccca agccaaaccg cctgttgcaa aaattatgga
      421 ctacggtaag ttcaaatttg agtaccagaa gaagcaaaaa gaacaacgta aaaaacaaag
      481 cgttgttact gtgaaagaag ttcgtctaag tccaactatt gacaagggtg actttgatac
      541 aaaacttcgt aatgcacgta agttccttga aaaaggaaat aaagttaagg tatctattcg
      601 ctttaagggt cgtatgatta cccataaaga gattggtgca aaagttttag ccgagtttgc
      661 tgaagcaact caagatattg ccatcatcga acaacgtgct aaaatggatg gacgtcaaat
      721 gttcatgcaa ttggcgccag cgactgacaa aaaataattg tcagaaagtt aaataaagga
      781 gaaaaaatca tgccaaaaca aaaaacacac cgcgcatcag ctaaacgttt caaacgtaca
      841 ggttctggtg gacttaaacg tttccgtgct tacacttctc accgtttcca cggaaaaact
      901 aagaaacaac gtcgtcatct tcgtaaagca tctatggtgc attcaggaga ttacaaacgt
      961 atcaaagcaa tgcttactcg cttgaaataa tagcttgact gtaagtaaac aattctagga
     1021 aatatttgga ggaaataaaa catggcacgt gttaaaggtg gcgttgtatc acgcaaacgt
     1081 cgtaaacgta ttcttaaatt agcaaaaggt tactatggag ctaaacacat cttgttccgt
     1141 actgcaaaag aacaagtaat gaactcttac tactatgcat accgtgaccg tcgtcagaaa
     1201 aaacgtgact tccgcaaatt gtggattact cgtatcaatg cggcagctcg tatgaacgga
     1261 ctttcatact cacaattgat gcatggtttg aaattggctg agattgaagt taaccgtaaa
     1321 atgcttgctg acttggctgt taacgatgca gtagctttca cagctcttgc agatgcagct
     1381 aaagcaaaac ttggtaaata acaactgatg agactggaac aggattgtcc agtcttttta
     1441 aaaataaagg agaaagatat ggtttcaaaa atgctacaca cttgcttacg agtagaaaat
     1501 cttgaaaaat caatcgcatt ttataaagat gcttttggtt ttaaagaatt gcgtcgcaga
     1561 gattttccag accatgcctt cacgattgtc tatctaggtc ttgagggtga tgattatgag
     1621 ttggagttga cttataacta cgatcacggt ccgtatgtgg ttggtgatgg gtttgcccat
     1681 atcgccctca gtacacctga tcttgaggca cttcatcaag aacacagtgc aaaaggctat
     1741 gaagtgactg agccaaatgg tctaccagga actacaccaa actattactt tgttaaagac
     1801 cctgatggat ataaggtcga agtcattcgt gaaaaataat ttcgtgcaag ttaagtagaa
     1861 tattcgtttt ctacttgact ttttttagct gaaagagtat actaataaaa attcaacctt
     1921 taaggggagt ccagagagac tcacaaggtg tcagataaaa gaatagtgca attttctaga
     1981 ggagaccttt tgagtgtgct ctcttgtgtt gtacgatttt aactgaggcc ttgcactagc
     2041 aaggtctttt ctttatctgg tccccttaaa atttaaggag gaaaagttat gaatctcaca
     2101 tgtaagaagc gtttgggtgt cattcggttg gaaaccatga aggtggttgc acaagaggaa
     2161 atcgcgccag caatctttga attagtccta gaaggagaaa tggttgaagc catgcgagca
     2221 ggccaatttc ttcatctgcg tgtaccagac gatgcccatc tcttacgtcg tcctatttca
     2281 atttcgtcta ttgacaaggc aaacaagcag tgtcacctca tttatcggat tgagggagct
     2341 gggactgcaa ttttttcaac cttaagtcag ggagacactc ttgatgtgat ggggcctcag
     2401 ggaaatggtt ttgacttgtc tgaccttgat gagcagaatc aggttctcct tgttggtggt
     2461 gggattggtg ttccaccctt gcttgaggtg gccaaggaat tgcatgaacg tggagtgaaa
     2521 gtagtgacag tcctcggttt tgctaataag gatgctgtta ttttgaaaac ggaattggct
     2581 cagtatggtc aggtctttgt aacgacagat gatggttctt atggcatcaa gggaaatgtt
     2641 tccgttgtta tcaatgattt agatagtcag tttgatgctg tttactcgtg tggggctcca
     2701 ggaatgatga agtatatcaa tcaaaccttt gatgatcacc caagagccta tttatctctg
     2761 gaatctcgta tggcttgtgg gatgggagct tgctatgcct gtgttctaaa agtaccagaa
     2821 agcgagacgg tcagccaacg cgtctgtgaa gatggtcctg ttttccgcac aggaacagtt
     2881 gtattataag gagaaaatta tgactacaaa tcgattacaa gtgtctctac ctggtttgga
     2941 tttgaaaaat ccgattattc cagcatcagg ctgttttggc tttggacaag agtatgccaa
     3001 gtactatgat ttaaaccttt taggttctat tatgatcaag gcgacaaccc ttgaaccacg
     3061 ttttgggaac ccaactccaa gagtagcaga gacgcctgct ggtatgctca atgcaattgg
     3121 cttgcaaaat cctggtttag aggttgtttt ggctgaaaag ctaccttggc tggaaagaga
     3181 atatccaaat cttcctatta ttgccaatgt agctggtttt tcaaaacaag agtatgcagc
     3241 tgtttctcat gggatttcca aggcaactaa tgtaaaagct atcgagctca atatttcttg
     3301 tcccaatgtt gaccactgta atcatggact tttgattggt caagatccag atttggctta
     3361 tgatgtggtg aaagcagctg tggaagcctc agaagtgcca gtttatgtca aattaacccc
     3421 gagtgtgacc gatatcgtta ctgtcgcaaa agctgcagaa gatgcgggag caagtggctt
     3481 gaccatgatc aatactctgg ttggaatgcg ctttgacctc aaaaccagaa aaccaatctt
     3541 ggccaatgga acaggtggaa tgtcaggtcc agcagtcttt ccagtagccc tcaaactcat
     3601 ccgccaagta gcccaaacaa cagacctgcc tatcattgga atggggggag tggattcggc
     3661 tgaagctgcc ctagaaatgt atctggctgg ggcatctgct atcggagttg gaacagctaa
     3721 ctttaccaat ccttatgcct gccctgacat catcgaaaat ttaccaaaag tcatggataa
     3781 atacggtatt agcagtctgg aagaactccg tcaggaagta aaagagtttc tgaggtaaac
     3841 tgcaatcaat ctgttcttga ttttttatta gtttgtaata tgaatttagg agaattttgg
     3901 tacaataaaa taaataagaa cagaggaaga aggttaatga agaaagtaag atttattttt
     3961 ttagctctgc tatttttctt agctagtcca gaaggtacaa tggctagtga tggtacttgg
     4021 caaggaaaac agtatctgaa agaagatggc agtcaagcag cgaatgagtg ggtttttgat
     4081 actcattatc aatcttggtt ctatataaaa gcagatgcta actatgctga aaatgaatgg
     4141 ctaaagcaag gtgacgacta tttttacctc aaatctggtg gctatatggc caaatcagaa
     4201 tgggtagaag acaagggagc cttttattat cttgaccaag atggaaagat gaaaagaaat
     4261 gcttgggtag gaacttccta tgttggtgca acaggtgcca aagtaataga agactgggtc
     4321 tatgattctc aatacgatgc ttggttttat atcaaagcag atggacagca cgcagagaaa
     4381 gaatggctcc aaattaaagg gaaggactat tatttcaaat ccggtggtta catgacagcc
     4441 aatgaatgga tttgggataa ggaatcttgg ttttatctca aatctgatgg gaaaatagct
     4501 gaaaaagaat gggtctacga ttctcatagt caagcttggt actacttcaa atccggtggt
     4561 tacatgacag ccaatgaatg gatttgggat aaggaatctt ggttttacct caagtctgat
     4621 ggcaaaatgg ctgaaaaaga atgggtctac gattctcata gtcaagcctg gtactacttc
     4681 aaatctggtg gctacatggc gaaaaatgag acagtagatg gttatcagct tggaagcgat
     4741 ggtaaatggc ttggaggaaa agctacaaat aaaaatgctg cttactatca agtagtgcct
     4801 gtcacagcca atgtttatga ttcagatggt gaaaagcttt cctatatatc gcaaggtagt
     4861 gtcgtatggc tagataagga tagaaaaagt gatgacaagc gcttggctat tactatttct
     4921 ggtttgtcag gctatatgaa aacagaagat ttacaagcgc tagatgctag taaggacttt
     4981 atcccttatt atgagagtga tggccaccgt ttttatcact atgtggctca gaatgctagt
     5041 atcccagtag cttctcatct ttctgatatg gaagtaggca agaaatatta ttcggcagat
     5101 ggcctgcatt ttgatggttt taagcttgag aatcccttcc ttttcaaaga tttaacagag
     5161 gctacaaact acagtgctga agaattggat aaggtattta gtttgctaaa cattaacaat
     5221 agccttttgg agaacaaggg cgctactttt aaggaagccg aagaacatta ccatatcaat
     5281 gccctttatc tccttgccca tagtgcccta gaaagtaact ggggaagaag taaaattgcc
     5341 aaagataaga ataatttctt tggcattaca gcctatgata cgacccctta cctttctgcc
     5401 aagacatttg atgatgtgga taagggaatt ttaggtgcaa ccaagtggat taaggaaaat
     5461 tatatcgata ggggaagaac tttccttgga aacaaggctt ctggtatgaa tgtggaatat
     5521 gcttcagacc cttattgggg cgaaaaaatt gctagtgtga tgatgaaaat caatgagaag
     5581 ctaggtggca aagattagta ctataagtga atatgatttg agtgaatagt aagttaaaaa
     5641 tcctgatttc aagtaaaatc aggatttttt catggatgca atttttttgg agtctggtgt
     5701 gacgcggagg gtcttttgtc ctgtgtaagt gacaaagccg ggttttccac cagttggttt
     5761 attgagtttt ttgacttcaa tcatatctac ctgcaccaga ttcgacaggc gcccttgaga
     5821 gaagtaggca gctaactctg ctgcgtctgt cttgactgca tcagatgggt caagatttcc
     5881 tgagatgaca acatggcttc caggaatgtc cttagcatgg aaccaaagtt cctccttgcg
     5941 ggccatttta aaggtcaatt cctcattttg aagattgttt cgtccgacat agatgatggt
     6001 tttgccatcg cttgctagat attgttctag ttttttgcgt ttctggattt tctcccgttg
     6061 tcttctgcgg ataaaacctg tttgaatcaa ttcttcacgg atttcagcga tttcttccag
     6121 tccagcttgg ttgaggacgg tttctacact ttccagatag agaatagtgg ctttggtttc
     6181 ttcaatcaaa tcagtcaagt atttgacagc ttctttgagt ttctgatacc gtttaaaata
     6241 gcgttgggca ttctggttgg gagtcagagc cttatcaagc gcaatcatga taggttggtt
     6301 ggtatagtag ttgtctagga taacctggtc ttggtcgtta ggcacttggt gtaggaaggt
     6361 tgtcagcaat tctccttttt gacgaaattc ttcagcgttg tctgtcgcca ataactcttt
     6421 ttcctgtttt ttgagtttgt gtcggttttt ctgaagttca ttttcaacac gacgaatcag
     6481 ttcactggcc tgctgtttga cgcggtcgcg ctcagcctta tccttatagt aggtgtccaa
     6541 caaatcagaa agatttgcaa aaggctctcc cacctgattt gcaaaaggaa ctggactgaa
     6601 ggaagtctta gtcaagcatg gcttggtttc ttgattgaaa aaatttcgga aagcggaaag
     6661 tttttcacta accagtatcc tttccaattc atttgccgta tcgcgtccca gaccttgaaa
     6721 gaggctttga agattttttg ctgttagttc ttgggtttgc aggatttcaa agagcttttc
     6781 atccttgata gtaaaaggat tgagagattc tgtacttggc ggagcgatat aggtcgatcc
     6841 tggaagtaag gtgcggtagc tattttgtga aaaaccgacg tgtttgataa cttcgatgat
     6901 tttatgactg cttttatcga ccagtagaat attactgtgt ttccccataa tttcaataat
     6961 caaggtagcc tggatatggt ctccaatctc gtttttattg gaaactgtaa tttccacaat
     7021 acggtcattt tccacttgct caatcgactc aatcagggcc ccctgcaaat actttctcaa
     7081 aaccatgata aaggtagaag gttgagctgg attttcaaaa gtcgtttggg tcagctgaat
     7141 gcgtccaaaa actggatggg cagaaaggag caggcgatgg ctttggcgat tgctgcggat
     7201 ttgcaagacc aactcttgtt caaaaggctg attgattttc tggatgcgac cattcactaa
     7261 ttcgcttcgc aattcctcaa ctatgtggtg taaaaaaaat ccatcaaatg acatcgttct
     7321 ctccttgtga ttgtattcca tagtattata tcaaaaaggt agaataaaat catagaaatg
     7381 tggtataata aagccaagta aagagaaacg agaagcacat gtatattgaa atggtagatg
     7441 aaactggtca agtttcaaaa gaaatgttgc aacaaaccca agaaattttg gaatttgcag
     7501 cccaaaaatt aggaaaagaa gacaaggaga tggcagtcac ttttgtgacc aatgagcgta
     7561 gtcatgaact taatctggag taccgtgaca ccgaccgtcc gacagatgtc atcagccttg
     7621 agtataaacc agaattggaa attgcctttg acgaagagga tttgcttgaa aatccagaat
     7681 tggcagagat gatgtctgag tttgatgcct atattgggga attgttcatc tctatcgata
     7741 aggctcatga gcaggctgaa gaatatggtc acagctttga gcgtgagatg ggcttcttgg
     7801 cagtacacgg ctttttacat attaacggct atgatcacta cactccggaa gaagaagcgg
     7861 agatgttcgg tttacaagaa gaaattttga cagcctatgg actcacaaga caataaacga
     7921 aaatggaaaa atcgtgactt gatatccagt ttagaatttg ctttgacagg tatttttact
     7981 gctatcaagg aagaacgcaa tatgcgaaaa cacgcagtga cggctctagt ggtcatcctt
     8041 gcaggttttg tttttcaggt gtcacgaatc gaatggctct ttctcctatt gaatattttc
     8101 ttggtagtag cctttgagat tatcaactct gctattgaaa atgtggtgga tttggccagt
     8161 cactatcact tttccatgct ggctaaaaat gccaaggata tggcggccgg cgcggtatta
     8221 gtggtttctc ttttcgcagc cttaacaggc gcattgattt ttctcccacg aatctgggat
     8281 ttattatttt aaacagtaag aggaaattat gacttttaaa tcaggctttg tagccatttt
     8341 aggacgtccc aatgttggga agtcaacctt tttaaatcac gttatggggc aaaagattgc
     8401 catcatgagt gacaaggcgc agacaacgcg caataaaatc atgggaattt acacgactga
     8461 taaggagcaa attgtcttta tcgacacacc agggattcac aagcctaaaa cagctctcgg
     8521 agatttcatg gttgagtctg cctacagtac ccttcgcgaa gtggacaccg ttcttttcat
     8581 ggtgcctgct gatgaagcgc gtggtaaggg ggacgatatg attatcgagc gtctcaaggc
     8641 tgccaaggtt cctgtgattt tggtggtgaa taaaatcgat aaggtccatc cagaccagct
     8701 cttgtctcag attgatgact tccgtaatca aatggacttt aaggaaattg ttccaatctc
     8761 agcccttcag ggaaataacg tgtctcgtct agtggatatt ttgagtgaaa atctggatga
     8821 aggtttccaa tatttcccgt ctgatcaaat cacagaccat ccagaacgtt tcttggtttc
     8881 agaaatggtt cgcgagaaag tcttgcacct aactcgtgaa gagattccgc attctgtagc
     8941 agtagttgtt gactctatga aacgagacga agagacagac aaggttcaca tccgtgcaac
     9001 catcatggtc gagcgcgata gccaaaaagg gattatcatc ggtaaaggtg gcgctatgct
     9061 taagaaaatc ggtagcatgg cccgtcgtga tatcgaactc atgctaggag acaaggtctt
     9121 cctagaaacc tgggtcaagg tcaagaaaaa ctggcgcgat aaaaagctag atttggctga
     9181 ctttggctat aatgaaagag aatactaagt agaggtaggc tcatgcctgc ttcttgtttt
     9241 tacagaagga ggacttatgc ctgaattacc tgaggttgaa accgtttgtc gtggcttaga
     9301 aaaattgatt ataggaaaga agatttcgag tatagaaatt cgctacccca agatgattaa
     9361 gacggatttg gaagagtttc aaagggaatt gcctagtcag attatcgagt caatgggacg
     9421 tcgtggaaaa tatttgcttt tttatctgac agacaaggtc ttgatttccc atttgcggat
     9481 ggagggcaag tatttttact atccagacca aggacctgaa cgcaagcatg cccatgtttt
     9541 ctttcatttt gaagatggtg gcacgcttgt ttatgaggat gttcgcaagt ttggaaccat
     9601 ggaactcttg gtgcctgacc ttttagacgc ctactttatt tctaaaaaat taggtcctga
     9661 accaagcgaa caagactttg atttacaggt ctttcaagct gcccttgcca agtccaaaaa
     9721 gcctatcaaa tcccatctcc tagaccagac cttggtagct ggacttggca atatctatgt
     9781 ggatgaggtt ctctggcgag ctcaggttca tccagctaga ccttcccaga ctttgacagc
     9841 agaagaagcg actgccattc atgaccagac cattgctgtt ttgggccagg ctgttgaaaa
     9901 aggtggctcc accattcgga cttataccaa tgcctttggg gaagatggaa gcatgcagga
     9961 ctttcatcag gtctatgata agactggtca agaatgtgta cgctgtggta ccatcattga
    10021 gaaaattcaa ctaggcggac gtggaaccca cttttgtcca aactgtcaaa ggagggactg
    10081 atgggaaaaa tcatcggaat cactggggga attgcctctg gtaagtcaac tgtgacaaat
    10141 tttctaagac agcaaggctt tcaagtagtg gatgccgacg cagtcgtcca ccaactacag
    10201 aaacctggtg gtcgtctgtt tgaggctcta gtacagcact tttggcaaga aatcattctt
    10261 gaaaacggag aactcaatcg ccctctccta gctagtctca tcttttcaaa tcctgatgaa
    10321 cgagaatggt ctaagcaaat tcaaggggag attatccgtg aggaactggc tactttgaga
    10381 gaacagttgg ctcagacaga agagattttc ttcatggata ttcccctact ttttgagcag
    10441 gactacagcg attggtttgc tgagacttgg ttggtctatg tggaccgaga tgcccaagta
    10501 gaacgcttaa tgaaaaggga ccagttgtcc aaagatgaag ctgagtctcg tctggcagcc
    10561 cagtggcctt tagaaaaaaa gaaagatttg gccagccagg ttcttgataa taatggcaat
    10621 cagaaccagc ttcttaatca agtgcatatc cttcttgagg gaggtaggca agatgacaga
    10681 gattaactgg aaggataatc tgcgcattgc ctggtttggt aattttctga caggagccag
    10741 tatttctttg gttgtacctt ttatgcccat cttcgtggaa aatctaggtg tagggagtga
    10801 gcaagtcgct ttttatgcag gcttagcaat ttctgtctct gctatttccg cggcgctctt
    10861 ttctcctatt tggggtattc ttgctgacaa atacggccga aaacccatga tgattcgggc
    10921 aggtcttgct atgactatca ctatgggagg cttggccttt gtcccaaata tctattggtt
    10981 aatctttctt cgtttactaa acggtgtatt tgcaggtttt gttcctaatg caacggcact
    11041 gatagccagt caggttccaa aggagaaatc aggctctgcc ttaggtactt tgtctacagg
    11101 cgtagttgca ggtactctaa ctggtccctt tattggtggc tttatcgcag aattatttgg
    11161 cattcgtaca gttttcttac tggttggtag ttttctattt ttagctgcta ttttgactat
    11221 ttgctttatc aaggaagatt ttcaaccagt agccaaggaa aaggctattc caacaaagga
    11281 attatttacc tcggttaaat atccctatct tttgctcaat ctctttttaa ccagttttgt
    11341 catccaattt tcagctcaat cgattggccc tattttggct ctttatgtac gcgacttagg
    11401 gcagacagag aatcttcttt ttgtctctgg tttgattgtg tccagtatgg gcttttccag
    11461 catgatgagt gcaggagtca tgggcaagct aggtgacaag gtgggcaatc atcgtctctt
    11521 ggttgtcgcc cagttttatt cagtcatcat ctatctcctc tgtgccaatg cctctagccc
    11581 ccttcaacta ggactctatc gtttcctctt tggattggga accggtgcct tgattcccgg
    11641 ggttaatgcc ctactcagca aaatgactcc caaagccggc atttcgaggg tctttgcctt
    11701 caatcaggta ttcttttatc tgggaggtgt tgttggtccc atggcaggtt ctgcagtagc
    11761 aggtcaattt ggctaccatg ctgtctttta tgcgacgagc ctttgtgttg cctttagttg
    11821 tctctttaac ctgattcaat ttcgaacatt attaaaagta aaggaaatct agtgcgagta
    11881 aaaattaatc tcaaatgctc ctcttgtggc agtatcaatt acctaaccag taaaaattca
    11941 aaaacccata tagacaagat tgaggtttta aagtattgtc ccaaggaaag aaaagtaacc
    12001 ctacatcttg aatctaagta gattttatgg taaaataata gggattttaa ggagtttgat
    12061 atgtataacc tattattaac cattttatta gtattatctg ttgtgattgt gattgcaatt
    12121 ttcatgcaac caaccaaaaa ccaatccagc aatgtatttg atgccagttc aggtgatttg
    12181 tttgaacgca gtaaagctcg cggttttgaa gctgtaatgc agcgtttgac agggatttta
    12241 gtctttttct ggctagccat tgccttagca ttgacggtat tatcaagtag ataagaaaat
    12301 aatgggcagg actaggtctt tgcctctttt tatttttaaa ggatgtttga gaaggtttta
    12361 cagtaaaaga aaattaaaaa atctagaaag aaaatatgaa agatagaata aaagaatatt
    12421 tacaagacaa gggaaaggtg actgttaatg atttggctca ggctttggga aaagacagtt
    12481 ccaaggattt tcgtgagttg attaaaacct tgtccttaat ggaaagaaag caccagattc
    12541 gctttgaaga agatggcagt ctgaccttgg aagccaagaa aaaacatgag attaccctca
    12601 aggggatttt tcatgcccat aaaaatggtt ttggttttgt cagcctagaa ggggaagagg
    12661 acgacctttt tgtagggaaa aatgatgtca actatgctat tgatggtgat accgtcgagg
    12721 tagtgattaa gaaagtcgct gaccgcaata agggaacagc agcagaagcc aaaattattg
    12781 atatcctaga acacagtttg acaacagttg tcgggcaaat cgttctggat caggaaaagc
    12841 ctaagtatgc tggctatatt cgttcaaaaa atcagaaaat cagtcaaccg atttatgtta
    12901 agaaaccagc cctaaagtta gaaggaacag aagttctcaa ggtctttatc gataaatacc
    12961 caagcaagaa acatgatttc tttgtcgcga gtgttctcga tgtggtggga cactcaacgg
    13021 atgtcggaat tgatgttctt gaggttttgg aatcaatgga cattgtatcc gagtttccag
    13081 aagctgttgt taaggaagca gaaagtgtgc ctgatgctcc gtctcaaaag gatatggaag
    13141 gtcgtctgga actaagagat gaaattacct ttaccattga cggtgcggat gccaaggact
    13201 tggacgatgc agtgcatatc aaggctctga aaaatggcaa tctggagctt ggggttcaca
    13261 tcgcagatgt ttcttattat gtgaccgagg ggtctgccct tgacaaggaa gcccttaacc
    13321 gtgcgacttc tgtttacgtg acagaccgag tggtgccaat gcttccagaa cgactatcaa
    13381 atggcatctg ctctctcaat ccccaagttg accgcctgac ccagtctgct attatggaga
    13441 ttgataaaca tggtcgtgtg gtcaactata ccattacaca aacagttatc aagaccagtt
    13501 ttcgtatgac ctatagcgat gtcaatgata tcctagctgg cgatgaagaa aagagaaaaa
    13561 aatatcataa aattgtacca agtatcgaac tcatggccaa gcttcatgaa actttagaaa
    13621 acatgcgtgt gaaacgtgga gctctcaatt ttgataccaa tgaagcgaag attttagtgg
    13681 ataaacaagg taagcctgtt gatatcgttc ttcggcagcg tggtattgcc gagcggatga
    13741 ttgagtcttt tatgttgatg gctaatgaaa cagttgccga acatttcagc aagttggatt
    13801 tgccttttat ctatcgaatt cacgaggagc ctaaggctga aaaggttcag aagtttattg
    13861 attatgcttc gagttttggc ttgcgcattt atgggactgc cagtgagatt agtcaggagg
    13921 cacttcaaga catcatgcgt gctgttgagg gagaatctta tgcagatgta ttgtccatga
    13981 tgcttcttcg ctctatgcag caggctcgtt attcggagca caattacggc cactatggac
    14041 tagctgctga ctattatact cactttacca gtccaattcg tcgttatcca gaccttcttg
    14101 ttcaccgtat gattcgggat tacggccgtt ctaaggaaat agcagagcat tttgaacaag
    14161 tgattccaga gattgcgacc cagtcttcca accgtgaacg tcgtgccata gaagctgagc
    14221 gtgaagtcga agccatgaaa aaggctgagt atatggaaga atacgtgggt gaagagtatg
    14281 atgcagttgt atcaagtatt gtcaaattcg gtctctttgt cgaattgcca aacacagttg
    14341 aaggcttgat tcacatcact aatctacctg aattttatca tttcaatgag cgtgatttga
    14401 ctcttcgtgg agaaaaatca ggtatcactt tccgagtggg tcagcagatc cgtatccgtg
    14461 ttgaaagagc ggataaaatg actggagaga ttgatttttc attcgtacct agtgagtttg
    14521 atgtgattga aaaaggcttg aaacagtcta gtcgtagtgg cagagggcgt ggttcaaatc
    14581 gtcgttcgga taagaaggaa gacaagagaa aatcaggacg ctcaaatgat aagcgtaagc
    14641 attcacaaaa agacaagaag aaaaaaggaa agaaaccttt ttacaaggaa gtagctaaga
    14701 aaggagccaa gcatggcaaa gggcgaggga aaggtcgtcg cacaaaataa aaaggcacgc
    14761 cacgactata caatcgtaga tacgctagag gcagggatgg tcctgactgg aactgaaatc
    14821 aagagtgtac gagctgctcg aattaatctc aaggatggct ttgctcaagt gaaaaatgga
    14881 gaagtttggc tgagcaatgt tcatatcgcg ccttacgaag agggcaatat ctggaaccag
    14941 gaaccagaac gtcgtcgtaa actcctgctc cataaaaggc aaattcaaaa attggaacaa
    15001 gagaccaaag ggacaggaat gaccttagtt ccccttaagg tctatataaa agatggctac
    15061 gctaagcttc ttttaggact tgccaaaggg aagcatgact atgacaaacg ggagtctatc
    15121 aaacgtcgtg agcaaaatcg agatatcgcg cgtgtgatga aagctgttaa tcagcgataa
    15181 aaagaggaat tgaaaatgga aaaattagtt gcctataaac gcatgccttt gtggaataaa
    15241 caaacaatgc ctgaagctgt tcagcaaaag cacaatacaa aagttgggac ttgggggaaa
    15301 attactgtct tgaagggagc tctcaagttt attgaattga cagaagaagg ggaagttcta
    15361 gctgaacacc tctttgaagc aggggcagac aatccaatgg cccaacctca agcctggcac
    15421 cgagtggaag ctgccacaga tgatgtggaa tggtacttgg aattttattg taaacctgag
    15481 gattattttg ctaaaaaata caataccaat cctgttcatt cagaggtcct agaggccatg
    15541 gagacagtga aacaagggaa agctttggat ttgggttgtg gtcaggggcg taattctctt
    15601 tttctagccc agcaagattt tgatgtgacg gctgtagatc aaaatggact agctcttgaa
    15661 atcttgcaaa gcattgtgga gcaggaagat ttggacatgc ctgttggcct ttacgatatc
    15721 aattcagcta gcattgaaca agaatatgat tttatcgttt caacagttgt tctcatgttt
    15781 ctacaagcgg accgcattcc agctattatt caaaatatgc aggagaaaac cagtgttggt
    15841 ggttacaacc ttatcgtttg tgccatggac acggaggatt atccttgctc ggttaacttc
    15901 ccattcacct ttaaagaagg agaactggca gactattaca aggattggga attggttaag
    15961 tacaatgaaa atccaggcca tttgcatcgt cgcgatgaga atggcaatcg tattcaacta
    16021 cgctttgcga ccttactagc taagaaaatc aagtaaacac acatgaagat taggaatttt
    16081 cctgatcttt tttctttttt acgaatgata tagaaaagga gggaattcat gtttgttgcg
    16141 agagatgcta ggggagaatt ggtaaatgtg ttagaggata aacttgagaa gcaagcatac
    16201 acctgcccag cttgtggagg ccagctccat ttgcgtcaag gaccaagtgt acggacgcat
    16261 tttgcccata aatccttaaa agactgtgat tttttctttg aaaatgaaag tccagaacac
    16321 ctggccaata aggaatccct ctatcactgg ttgaaaaaag agacaaaggt tcaattagag
    16381 tacccgcttt cagaacttaa acagattgcg gatgtatttg taaatggcaa tctagctcta
    16441 gaagttcagt gtagtccctt gcctcagaaa gtccttaaag agcgaagtga gggctatcgt
    16501 agtcagggtt accaagtact gtggttgctg ggtcaaaaac tgtggctcaa ggagcgtttg
    16561 actcgtctac agcaaggttt tctttatttc agtcaaaaca tgggctttta tgtttgggaa
    16621 ttagacaagg aaaaacaagt tttaagactc aaatacctga tttaccagga tctccgcggt
    16681 aaactccatt atcaaatcaa ggaattttcc tatggtcaag gtagtttatt ggaaatattg
    16741 cgtcttccct ataagagaca aaaaatatct cattttacag tttctgagga caaggacatc
    16801 tgtcgctata tccggcaaca actttattat caaaatctct tttggatgaa agaacaagca
    16861 gaagcctatc aaaagggaga aaatatcctg acttatggac tgaaagaatg gtatccacaa
    16921 attcgaccaa tagtgggcaa atttttccag attgaacaag acttgactag ctattatcag
    16981 cacttttata cctattacca aaaaaatcct caaaatgatt ggcaaaagct ttatccacca
    17041 gccttttatc agcaatattt cttgaaaaat atggtagaat agaaaggatg gaggaatcta
    17101 atggtattac aaagaaatga aataaatgaa aaagatacat gggatctatc aacgatctac
    17161 ccaactgacc aggcttggga agaagcctta aaagatttaa cagaacaatt ggagacagta
    17221 gcccagtatg aaggccatct cttggatagt gcggataacc tactagaaat cactgaattt
    17281 tctcttgaaa tggaacgcca gatggagaag ctttacgttt atgctcatat gaagaatgac
    17341 caggatacac gtgaagctaa gtatcaagag tactatgcca aggccatgac actctacagc
    17401 cagttagacc aagccttttc attctatgat cctgaattta tggagattag cgaaaagcag
    17461 tatgctgact ttttagaagc tcaaccaaag ctgcaggttt atcaacacta ttttgacaag
    17521 ctcttgaaag gcaaggatca cgttctttca caacgtgaag aagaattatt ggctggagct
    17581 ggagaaatct ttggttcagc aagtgaaacc ttcgctatct tggacaatgc ggatattgtg
    17641 ttcccttatg tcctagacga tgatggtaaa gaagttcagc tatctcatgg gacttacaca
    17701 cgtttgatgg agtctaaaaa acgtgaggtt cgccgtggtg cctatcaagc tctttatgcg
    17761 acttacgaac aattccaaca cacctatgcc aaaaccttgc aaaccaatgt taaggtgcaa
    17821 aattaccgtg ctaaagttcg taactacaag agtgctcgtc atgcagccct cgcagcgaat
    17881 tttgttccag aaagtgttta tgacaatttg gtagcagcag ttcgcaagca tttgccactc
    17941 ttacatcgct atcttgagct tcgttcaaaa atcttgggga tttcagatct caagatgtac
    18001 gatgtctaca caccgctttc atctgttgaa tacagtttta cctaccaaga agccttgaaa
    18061 aaagcagaag atgctttggt agtcttgggt gaggattact tgagccgtgt taaacgtgcc
    18121 ttcagcgagc gttggattga tgtttacgaa aatcaaggca agcgttcagg tgcctactct
    18181 ggtggttctt acgataccaa tgcctttatg cttctcaact ggcaggacaa tctggacaat
    18241 ctctttactc ttgttcatga aacaggtcac agtatgcatt caagctatac tcgtgaaact
    18301 cagccttatg tttacggaga ttactctatc tttttggctg agattgcctc aactaccaat
    18361 gaaaatatct tgacggagaa attattggaa gaagtggaag acgacgcaac acgctttgct
    18421 attctcaata acttcctaga tggtttccgt ggaacagttt tccgccaaac tcaatttgct
    18481 gagtttgaac acgccattca ccaagcagat caaaatgggg aggtcttgac aagcgatttc
    18541 ctaaataaac tctacgcaga cttgaaccaa gagtattatg gtttgagtaa ggaagacaat
    18601 cctgaaatcc aatacgagtg ggctcgtatt ccacacttat actataacta ctatgtatat
    18661 caatattcaa ctggatttgc ggccgcctca gccttggctg aaaaaattgt ccatggtagt
    18721 caagaagacc gtgaccgcta tatcgactac ctcaaggcag gtaagtcgga ctatccactt
    18781 aatgtcatga gaaaagctgg tgttgatatg gagaaggaag actacctcaa cgatgccttt
    18841 gcagtctttg aacgccgttt aaatgagttt gaagcccttg ttgaaaaatt aggattggca
    18901 taaaatggtt gaatcgtata gtaagaatgc taaccataac atgcgtcgtc ctgtcgtcaa
    18961 agaagaaatt gtagacttga tgcgtcagcg tcaaaagcaa gtcacaggtt ccttgaaaga
    19021 attggaagac tttgcccgca aggaaaatat tcctattatt ccccatgaaa cggttgctta
    19081 tttccgtttt cttatggaaa ccatgcagcc taaaaatatt ctggaaattg ggacggctat
    19141 cggtttttca gctctcttga tggctgaaca tgcgccaaat gctaagatta caactattga
    19201 tcgtaatcca gaaatgattg gttttgccaa ggaaaatttt gcccagtttg acagtcgcaa
    19261 gcaaatcact ctcctagagg gagatgcggt ggatgtctta tctacactga cagagtctta
    19321 tgatttcgtc tttatggatt ctgccaagtc taaatacatc gtctttctgc cagaaatcct
    19381 caaacatttg gaagttggtg gtgtggttgt cttggatgat atttttcaag gtggtgatgt
    19441 tgccaaggat attatggaag tccgtcgtgg tcagcgaacc atttatcgag gccttcaaaa
    19501 attatttgat gcaaccttag acaatccaga actcaccgca acattagtgc ctttaggaga
    19561 tggtattctc atgcttcgta aaaatgtagc agatgttcaa ctgtctgaaa gcgaatgatt
    19621 ttcagaaaaa tttaagaaaa aatagtaaaa tagatagagt aacacttatc tcaaaggagt
    19681 agacatgaag aaaaaattat tggcaggtgc catcacacta ttatcagtag caactttagc
    19741 agcttgttcg aaagggtcag aaggagcaga ccttatcagc atgaaagggg atgtcatcac
    19801 agaacatcaa ttttatgagc aagtgaaaaa caacccttca gcccaacaag tcttgttaaa
    19861 tatgaccatc caaaaagttt ttgaaaaaca atatggctca gagcttgatg ataaagaggt
    19921 tgatgatact attgccgaag aaaaaaaaca atatggcgaa aactaccaac gtgtcttgtc
    19981 acaagcaggt atgactcttg aaacacgtaa agctcaaatt cgtacaagta aattagttga
    20041 gttggcagtt aagaaggtag cagaagctga attgacagat gaagcctata agaaagcctt
    20101 tgatgagtac actccagatg taacggctca aatcatccgt cttaataatg aagataaggc
    20161 caaagaagtt ctcgaaaaag ccaaggcaga aggtgctgat tttgctcaat tagccaaaga
    20221 taattcaact gatgaaaaaa caaaagaaaa tggtggagaa attacctttg attctgcttc
    20281 aacagaagta cctgagcaag tcaaaaaagc cgctttcgct ttagatgtgg atggtgtttc
    20341 tgatgtgatt acagcaactg gcacacaagc ctacagtagc caatattaca ttgtaaaact
    20401 cactaagaaa acagaaaaat catctaatat tgatgactac aaagaaaaat taaaaactgt
    20461 tatcttgact caaaaacaaa atgattcaac atttgttcaa agcattgtcg gaaaagaatt
    20521 gcaagcagcc aatatcaagg ttaaggacca agccttccaa aatatcttta cccaatatat
    20581 cggtggtgga gattcaagct caagcagtag tacatcaaac gaatagtcca aatcaatgag
    20641 tcagggaaaa aactcgactt caggaaaaaa tgaagcaaac attcccacaa taaaacgcat
    20701 agtacaaggt ttgtactgcc ccccccaaaa gttagacaat taatttatcc gaaggattta
    20761 gttctgtatt gcacagagct aagtcctttt agttttatct taattctctt gttgttgtaa
    20821 taatcaatat agtctataat ggcttgttcc aattgattaa gtgatttaaa tgttttctca
    20881 tagccataaa acatttcgga ttttaaaata ccaaagaaag attccatcct accgttgtct
    20941 tggctgtttc cctttcgtga catagatgct tgaattccct tactctctag gaagcgatga
    21001 taagaatcgt gttggtattg ccagccttgg tcactatgga gaatcgtatt ctcatagtgc
    21061 ttctctttga atgcctgttc caacattgtt tgtacttgtt ctaagttggg tgaagttgaa
    21121 agattatagg cgataatttc actgttaaag ccatctaaaa ctggtgataa gtaaagcttt
    21181 tgagtacttg ctggaatggc aaattctgtc acatctgtgt agcacttttc cattgtttta
    21241 gagccttcaa attggccttg aatgagattc tctgctttct taccaacgtc tcctttatga
    21301 gaagaatatt ttcgtttctg tcgcatttta gcttgtaaat tgagtacttt catcaagcct
    21361 tgaactcttt tatgatttac cagataagca cgatttctta gttctaaatg aatacagcga
    21421 taagcataat ttcccttgtg ttcgataaaa atggattgaa tttcagcttt aagctcttgg
    21481 tccttatctg ttttgtctag ctgtttcaag tgatagtagt aggtccaacg agctagttta
    21541 atggctttta gaagaagatc taacgaaaac tcagtcatta attcttgaac aatttctgtc
    21601 tttctctttt tcctccttca atcggagttc tcttaacttt tttaggatgg cattctccgc
    21661 tctcaggtat tcattttctg cttgaagacg ttctaattct gtcctctctt caggtctcgt
    21721 ttttggctta cgtcccattt taggtactct ccctcttggt ttctcaacaa tagtataccc
    21781 gtttttctgt attgtgctag ccagttaaga agtatcgtac gacttgggag accgtattca
    21841 agagaaactc tatctttagt ccagccttca tgtcagactt tatta
//
LOCUS       DBERMR010000036        21424 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.036, whole genome shotgun sequence.
ACCESSION   DBERMR010000036 DBERMR010000000
VERSION     DBERMR010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 21424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21424
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.036"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(331..1179)
                     /gene="dprA"
                     /locus_tag="ACL8A1_001457"
     CDS             complement(331..1179)
                     /gene="dprA"
                     /locus_tag="ACL8A1_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HGS1202211.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            complement(1276..1965)
                     /locus_tag="ACL8A1_001458"
     CDS             complement(1276..1965)
                     /locus_tag="ACL8A1_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HGS1202212.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYVSGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            complement(1977..2855)
                     /locus_tag="ACL8A1_001459"
     CDS             complement(1977..2855)
                     /locus_tag="ACL8A1_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGS1202213.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAATHD
                     FLSIFILLAFLLVKEGKVRVSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFVAVANGQL
                     LGLMIVFVAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAALFLGTSLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(2845..3714)
                     /gene="licA"
                     /locus_tag="ACL8A1_001460"
     CDS             complement(2845..3714)
                     /gene="licA"
                     /locus_tag="ACL8A1_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HGS1202214.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGADFGYYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            complement(3731..4753)
                     /locus_tag="ACL8A1_001461"
     CDS             complement(3731..4753)
                     /locus_tag="ACL8A1_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribitol-5-phosphate dehydrogenase"
                     /protein_id="HGS1202215.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPEAEIVVIGRHWEKLELFSFAKECYITDNI
                     PEDLAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFENAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(4758..5465)
                     /locus_tag="ACL8A1_001462"
     CDS             complement(4758..5465)
                     /locus_tag="ACL8A1_001462"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HGS1202216.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKKIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            5801..7288
                     /gene="tacF"
                     /locus_tag="ACL8A1_001463"
     CDS             5801..7288
                     /gene="tacF"
                     /locus_tag="ACL8A1_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HGS1202217.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVAPEYYLLTTML
                     SETSLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            7298..8101
                     /locus_tag="ACL8A1_001464"
     CDS             7298..8101
                     /locus_tag="ACL8A1_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HGS1202218.1"
                     /translation="MKQLTIEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLTFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            8103..8912
                     /locus_tag="ACL8A1_001465"
     CDS             8103..8912
                     /locus_tag="ACL8A1_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HGS1202219.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGSIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
     gene            complement(9188..12364)
                     /gene="carB"
                     /locus_tag="ACL8A1_001466"
     CDS             complement(9188..12364)
                     /gene="carB"
                     /locus_tag="ACL8A1_001466"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HGS1202220.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKEGPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDTLIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDKVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
     gene            complement(12677..13756)
                     /locus_tag="ACL8A1_001467"
     CDS             complement(12677..13756)
                     /locus_tag="ACL8A1_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HGS1202221.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            complement(13806..14729)
                     /locus_tag="ACL8A1_001468"
     CDS             complement(13806..14729)
                     /locus_tag="ACL8A1_001468"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HGS1202222.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGSELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            complement(14748..15269)
                     /gene="pyrR"
                     /locus_tag="ACL8A1_001469"
     CDS             complement(14748..15269)
                     /gene="pyrR"
                     /locus_tag="ACL8A1_001469"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HGS1202223.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            complement(15480..16109)
                     /gene="nth"
                     /locus_tag="ACL8A1_001470"
     CDS             complement(15480..16109)
                     /gene="nth"
                     /locus_tag="ACL8A1_001470"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HGS1202224.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            complement(16109..16651)
                     /locus_tag="ACL8A1_001471"
     CDS             complement(16109..16651)
                     /locus_tag="ACL8A1_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HGS1202225.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNHLNQEILDDD
                     LVLPIENEALDLAESVSDNILLNIPIKVLTAEEEAGQGFVSGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            16821..17138
                     /locus_tag="ACL8A1_001472"
     CDS             16821..17138
                     /locus_tag="ACL8A1_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HGS1202226.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EKLNNSILIDLEKFYQPTSLLIGLGSLKLNDQARTAWRNYDKFHYEHIKHVLSLYGPV
                     FGF"
     gene            17377..18936
                     /locus_tag="ACL8A1_001473"
     CDS             17377..18936
                     /locus_tag="ACL8A1_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS1202227.1"
                     /translation="MHYEHSRKGNHMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKILMGEALSDFTIKGNIQSDYQSLAYIPQKLPEELKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQKIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRTYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLKQLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYISKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEICSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(19085..19984)
                     /gene="htpX"
                     /locus_tag="ACL8A1_001474"
     CDS             complement(19085..19984)
                     /gene="htpX"
                     /locus_tag="ACL8A1_001474"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HGS1202228.1"
                     /translation="MLFDQIASNKRKTWILLVVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALVSAITMLSGMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(19986..20546)
                     /locus_tag="ACL8A1_001475"
     CDS             complement(19986..20546)
                     /locus_tag="ACL8A1_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HGS1202229.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNIKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            20640..21353
                     /gene="rsmG"
                     /locus_tag="ACL8A1_001476"
     CDS             20640..21353
                     /gene="rsmG"
                     /locus_tag="ACL8A1_001476"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HGS1202230.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
BASE COUNT         6532 a         4527 c         3949 g         6416 t
ORIGIN      
        1 tcactatact ttcccttata ctcaatgaaa atcaaagcgc aaactaggaa gctagccaca
       61 ggttgctcaa agcactgctt tgaggttgta gataagactg acgaagtcag ctcaaaatac
      121 tgttttgagg ttacagatgg aagctgacgt agtttgaaga gattttcgaa gagtattata
      181 tgagaaaaat cctctactct actaatccac ttagaaaaaa cgcatattat caagtgttaa
      241 aaacttgcta atatgcgttt tattatggaa atgttgggtt cattttctct taaaattgat
      301 tttttcttag aattctagct taggtcattt ttaaaattca aattccgcaa gaacatcttg
      361 cccactggtg accaattttg ctccttcttg aatcaaatga tggcaaccgt ctgatagtcc
      421 atctaaaatg ctaccaggaa tagcaaaaac atcgcgtcct tcttccattg ctcgctcaca
      481 ggtaatgaga ctacctgaac gcatcttagc ctctgctaca atcacaccac gacaaagtcc
      541 agcaatgatg cgattacggg caggaaaatg aaatttcaga ggttgttcgc caggtccata
      601 ttcacttaga accagatggt cattgccgat gtagtcttgc aagcgtttat tggctttagg
      661 ataaaacaca tccagtcctg ttccaatcac tgcaatggtt tttccgccat tctgaagagc
      721 tgccatatga gctgctgtgt caatgccctt ggccagacca ctgacaataa ccagttcatt
      781 ttccaagcct tgaatgactt tttcaactga tttagctccc tgtttgctac aagcacgact
      841 gcccacgacc gctaccttcg ggaatttcag gaggtcaaga tttcccttgt aaaataaaag
      901 tacaggcgca tcatatattt cactcaaatc ccaaggataa cagtcatcta aaatagagaa
      961 agatggaaat ttttgaaact ctttcgacaa atgcgcatcg tctatctgaa aataacgctc
     1021 cataaaaaca gctggatttc ggcaacctga gatatctgca atatcaccca acaaaagctc
     1081 ctgatcaaca ttttcaccgt attctagcac tttcaaaatc tgttgattgg tcaaacctga
     1141 tttttttaac ttatagattt catagtttgt gattttcata aataactcca tttctttttc
     1201 tactcatcta tctattcgta aaagttcgta aaaaatcaaa aaaacatccg actattttgt
     1261 cagatgttgg aatctttaat tttcgttttt aagaatttct tctaatttac gatagtcttg
     1321 gacactatcg atctcataaa tgctattgcc ttctaattct tcaacataga catctagctc
     1381 tttgatatta tccttaacca tattgtccca gtagagatca acaaattcac cacttacata
     1441 agccttgtcg ataaagctga caatcttttc tgcagttgga gcatcccaga aggatacacc
     1501 actaaggatg cgacctgcct tgctatcaac aataatgtct tgaaccttgt agtcatctcc
     1561 atagaccaag aaccattcgt tggtacaatc ttcacgataa acactaaaat aagtcgaacg
     1621 tgtcaaatca ttgcggaaca tatttttaaa gagataattg tcagcatcaa taacatagct
     1681 gttggccaat tcttctttta caagatagag agagtaaaag ttattgtagt cagcgtattt
     1741 atcattgaaa acgaggcgaa caccgtattt ttcttttaag taatcgaatt gttctttaag
     1801 ataaccaaca atgatgatga tgtcattgat tcctttttct ttgagaaact caatttggta
     1861 ttcaatcaaa ggtttttgat taacctgaac caaggcttta ggggtatttt cagtcatagg
     1921 acgcaagcga gttcccaatc ccgctgctaa gataatggct ttcacacgaa tctcctttat
     1981 tctttaataa taatataaac tccagcaatg acgacaagtg acgtcataat ggtcagcata
     2041 tctaatgatg ttcccaagaa aagagctgcg aacaagacag tccatactac atagctcacg
     2101 ttcaagcctg tagccttggc tggttgcaag cgattgatag cgatataata agccaaatag
     2161 gaaatcatat caaaggctac aaagacaatc atgagaccta gcaattgtcc attggctact
     2221 gcaacaaatg actgatgaga gaagagcaca atcacaagat aggacaagaa cgaagttact
     2281 tgacggatta agagggcttc gatttcactc aattcacttt ccatggcaaa agagctaaga
     2341 acactctcac ttccccatgc aatagcacaa accaaagcac aaagaatccc aatgtagaaa
     2401 gaattaacct gttcaacctt ataggtctgg gcaataatcc ctccaataat caagacaatc
     2461 ccaaatacag tatttttcga aatcttgtgc ttcaaaaaga agaaagccaa tagaactgaa
     2521 atcgcggggt aaatagccga tacagatgaa gctaaagaac ttccgatata cttaactgca
     2581 taaagattgg cctgcatacc gatagggcct gctagcaagg ctccgatgat aacactgaca
     2641 ttgcgaatat ttaagaaaat tgagacgcga actttccctt cttttaccaa gagaaaagct
     2701 agtaagataa agatgctcaa aaaatcatga gttgcagcca ccacaaaggg tgacaaatct
     2761 gtaaaaatcg aaaagatata agcactaacc gttagaccca agccccagaa aatacctgag
     2821 agaaggccaa aaggaactcc gtttttactt ttcatctgaa cctccataag aagccaaacc
     2881 tttaacagct ctttggtaac gattcacacc atagtaacca aaatctgcac cttgctcttc
     2941 cttatataca gtccatagac tccaaatagt atcttgtaaa attttataaa tagcaatctt
     3001 ttcatgagaa accggtgttt ggtcactctc atagtgagat aagaaagttt cttcctcttg
     3061 ggaagtgaat tcagactcta aaaagagggc agccaaatcc cacattggat cattcattga
     3121 tgaatattcc cagtcaatca aataaagtcg tccttgaggt gattcgataa agttttcagg
     3181 caccaaatcg atatgacaag attttctgtc aacacctaag tcagccagtc ttttctctaa
     3241 ggagaagact gcatttctaa cagattcata gttggcataa ggaatttgtt cttcaatcaa
     3301 ggattcgtat tttttgattt cttcaaaagg agcaaattct cctcttaatt ccttagcaga
     3361 tgtatgaata gtttgtaata ttggagcaat tttgtcgaac ttggtcttga ttgacgttga
     3421 atcaagcgta atcgcagatt cgatatactc atttactttg ataccagctt caatatcaaa
     3481 aagataattt tttacatcta agcctaaatc ctttagtagt tcaagattgt acttttcatc
     3541 ttgtcgattg ataagctttt ctgtcccttt accaaagaat ttaacaatgt attgcttatt
     3601 tgttgttttg gccaaatagt tttgattggt cattccaccc agttgttcaa cactgaggac
     3661 ttcctcttct tgactaagta aggaagaaat tttttcttta atgattttct ccacaattaa
     3721 cctccagtac ttatacttcc cacttaaaca ctgttttaaa ggctgtgttt aaatcggttg
     3781 caaagacacg atgaatatct ttaatttctc ttacaggttc ttctagataa aggatatttt
     3841 taagacgatt ggcaaatttc ttgacttcca tcatttggat agcattttca aaatcaatgc
     3901 gaccagaacg agatgaccca accaaaatca agcccttttc taaggcatcg cgagtattga
     3961 gattgacttt atattcgcta actcccatca tgagaatcgt tccctgagga cgaatgtagc
     4021 gaatcaagtc attaatagct ggtccagtac catcaccacc acaacattca aaagcatggt
     4081 caaaggccaa atcttcagga atattatccg taatatagca ttctttggca aatgagaaga
     4141 gttccaactt ttcccaatga cgaccaataa ccacaatctc tgcttctggc aaagtatagt
     4201 tgataatatt ggcaaccaca aaagctaaac ttccatctcc aataacggcg atccgctccc
     4261 gcttgctatg agcaagagtc aatagacgat tcatagcgtg catgcccaca ctgacaaact
     4321 ctgtaatggc tgcaaccgta tcttcaatag catcataagc caccacacga tctttaggga
     4381 gagaaacaaa ctctctcata aagccatcaa atccactaga caagaaatgg gtccctgtca
     4441 tgtagttttc atagaattct tcatcactct gcataggaga ctgattggga atcatgacaa
     4501 ctttttgacc aacctcgtag gttccggtcg ggtcagaaat gacggttcca catgactcgt
     4561 gaatcattgc cattggaagc tttttattca aaatcttggg atcacgtttt ccctgatagt
     4621 aacgctgatc cgcatgacag acagccatgt agttgggacg gataaggata tgattctctt
     4681 ggtcaatagc ctcttcctga tatttgacat tgataaactt aggcttagtt agttgataaa
     4741 tttgattaat cattttacta gtctttctca atcatacttt ttgcaatctt caaatctgtt
     4801 acggttgtaa tcttcagatt tgagtattca cctttggcca aggccacatc ttttcctttg
     4861 atcacaaaga ttttacatgc atctgtcaag atttccttct cttcatcaga aagagatcca
     4921 taaaggtcca tgaagtcctt gcaacggaat gtttgaggtg tttgtccttg ataaaggtga
     4981 gcacgatttg gaatatctgt aataaattga ccattggtac tttcaacgat agtatcaacc
     5041 gcttctacca ctgtgtccac tgcgtcatga ttttgggcaa gttggatatt gtcctgaatc
     5101 atgcgaagtg taataaatgg acgaacagaa tcgtgggtaa caacgatatc ctctggagta
     5161 agcggacgat aagcatcaat ggcttcaatg attttcttaa tacttgtatt gcggtcagca
     5221 ccaccctttg taatgatgat acgttcctta taaagaggaa gatatttatc tacaagatct
     5281 tctgcatgag aaacccagtc tccatgaaca ccaactacaa ttttttcaat acttggctcc
     5341 aagacaaatt tttcaattgt atgaatcaaa ataggtcgat cacctagctc taaaaattgt
     5401 tttggcaagt tactgatccc catgcgtgtg ccagttccac cggcaagaat tcctgcataa
     5461 atcatctatt ttctcctttg tcttactaaa aaaacttatt ctctctatta taccacaatc
     5521 tagtcctatc ataaaagaaa tactggtcct ccccttctag aataggagaa ttatgcagtt
     5581 ctattattca aagaaactag tccatttcta gtaatgacta ggaattctgt agcaattact
     5641 aataaatggc tgcgaaattt tagagtcctt tagaataata tactttcctt aaagaaaact
     5701 taaaacttaa gatttgaaac ttcaaagtga ttacttgact tactgccctt catttcttat
     5761 tagctaactt tatgatataa tgaaaaacga ggtaaattga atgaaaagta taaaattaaa
     5821 tgctctatct tacatgggaa ttcgtgtctt gaatattatt tttcccatcc taactggaac
     5881 ctatgtcgcg cgtgtcttgg accgaactga ctatggttac ttcaactcag tcgacactat
     5941 tttgtcattt ttcttgccct ttgcaactta tggtgtctat aactacggtt taagggctat
     6001 cagtaatgtc aaggataaca aaaaagatct taacagaacc ttttctagtc ttttttattt
     6061 gtgcatcgct tgtacgattt tgaccactgc tgtctatatc ctagcctatc ctctcttctt
     6121 tactgataat ccaatcgtca aaaaggtcta ccttgttatg gggattcaac tcattgccca
     6181 gattttttca atcgaatggg tcaatgaagc tctggaaaat tacagttttc tcttttacaa
     6241 aactgccttc atccgtatcc tgatgctggt ctctattttc ttatttgtta aaaatgaaca
     6301 cgatattgtt gtctatacac ttgtgatgag tttatcgacg ctgattaact acctgattag
     6361 ttatttttgg attaaaagag acatcaaact tgttaagatt cacctaagtg attttaaacc
     6421 actctttctc cctctgacag ccatgttagt ctttgccaat gccaatatgc tcttcacttt
     6481 tttagatcgc ctcttcctcg ttaaaacagg gattgatgtc aacgttagtt actataccat
     6541 agctcagcga attgtgaccg ttatagctgg ggttgtaaca ggtgcaattg gagtgagtgt
     6601 gcctcgtctc agttactatc tggggaaagg agacaaagaa gcctatgttt ctctggttaa
     6661 tagaggtagt cgaatcttta acttctttat cattccactg agttttggac tcatggtttt
     6721 aggaccaaat gccatcctac tttacggtag tgaaaaatat atcggaggcg gcatcttgac
     6781 ctctctcttc gcttttcgta cgattatcct ggccttagat accattcttg gttcccaaat
     6841 tctctttacc aatggctatg aaaaacgtat cacagtctat acagtctttg ctgggctact
     6901 caatttgggc ttgaatagtc tcctcttttt caaccatatc gtggctcccg aatactactt
     6961 actgacaact atgttatcag agacttctct acttgttttc tatatcattt tcatccatag
     7021 aaaacaactc atccacttgg gacatatctt tagctatact gttcgatact ctctcttttc
     7081 actttccttt gtagcaattt atttcctgat taatttcgtg tatcctgtgg atatggtcat
     7141 taatttgcca tttttgatta atactggttt gattgtcttg ttatcagcta tctcttatat
     7201 tagtctactt gtcttcacaa aagatagcat tttctatgaa tttttaaacc atgtcctagc
     7261 cttaaaaaat aaattcaaaa aatcatagga gtttaaaatg aaacaactaa ccattgaaga
     7321 tgccaaacaa attgaattag aaattttgga ttatattgat actctctgta aaaagcacaa
     7381 tatcaactat attattaact acggtactct gattggggcg gttcgacatg agggctttat
     7441 cccttgggac gacgatattg atctgtccat gcctagagaa gactaccaac gatttattaa
     7501 catttttcaa aaggaaaaaa gcaagtataa gctcctatcc ttagaaactg ataagaacta
     7561 ctttaacaac tttatcaaga taaccgacag tacgactaaa attattgata ctcgaaatac
     7621 aaaaacctat gagtctggta tctttatcga tattttccct atagatcgct ttgatgatcc
     7681 taaggtcatt gatacttgtt ataaactgga aagcttcaaa ctgctgtctt tcagtaaaca
     7741 taaaaatatt gtctataagg atagcctttt aaaagattgg atacgaacag ccttttggtt
     7801 gctccttcga ccggtttctc ctcgttattt tgcaaataaa atcgagaaag aaattcaaaa
     7861 atatagtcgt gaaaatgggc agtatatggc ttttattcct tcaaaattta aggaaaagga
     7921 agtcttccca agtggtacct ttgataaaac aatcgattta acctttgaga atttaagcct
     7981 tcctgcacct gaaaaatttg atactatttt gacacaattt tatggagatt atatgaccct
     8041 accaccagaa gaaaaacgct tctacagtca tgaatttcac gcttataaat tggaggatta
     8101 ggatgcaata tttagaaaaa aaagaaatta aagaaattca actagccctg ctggactata
     8161 ttgatgagac ttgtaagaaa catgatattc cttattttct cagttatgga accatgcttg
     8221 gatccatccg ccacaaaggt atgattcctt gggatgatga tattgatatt tccctttatc
     8281 gtgaggatta tgagcgttta ctgaagatta ttgaagaaga aaatcaccct cgctacaagg
     8341 ttctttccta cgatacatct tcttggtact tccataattt cgcatcgatt ttggacactt
     8401 ctactgttat agaagaccat gttaagtaca agcgtcatga taccagcctt ttcatcgatg
     8461 tcttcccaat tgatcgattt acagacttga gcattgtcga caagagctat aagtatgtgg
     8521 ctcttcgtca actagcttat atcaaaaaat cacgagcagt tcacggtgat agcaaactaa
     8581 aagattttct tagattatgt agctggtacg ctctccgatt tgtcaatcct cgctactttt
     8641 acaagaaaat tgatcaacta gtcaaaaatg ctgtaaccaa cactcctcaa tatgaaggag
     8701 gagttgggat cggtaaggaa gggatgaaag aaatcttccc agttgatacc tttaaagaac
     8761 tgattttaac tgagtttgag ggccgtatgt tgcctgttcc caaaaaatat gaccaatttt
     8821 taacccagat gtatggcgat tatatgacac caccatcaaa agaaatgcaa gagtggtata
     8881 gtcatagcat taaagcttat cgcaaaaact gattgagggg gattatacag actactaaga
     8941 tagaggttat tcaaaaacat aattttagta gaaaatgaaa tacatattcc cacaataaaa
     9001 cgcatcatat caagtttttt gaaaaacctt gatatgatgc gttttataat tttaaagact
     9061 tttttctata gtagattgaa ataagatgcg aacaaatcaa ttagaaaatt caaattaatt
     9121 tctagaaata ttttagtatc cctgtgtact gttctaaatt cagtctgcta tatcttattt
     9181 tttctattta aatcgcttct gtaacaaagc tacgactttc aagtacctta agcatggcat
     9241 tagctgtatc tagcgctgtg aagaggggca ccccgtgttc aatggctgaa cgacgaattt
     9301 gctcaccatc ttcgtcagca gttcgttttg ttcctactgt gttaatgata gcttgaattc
     9361 ttcctttgcg tacaaaactt gggatatcct tatcgtcatc accaatctta ccaacaggtt
     9421 gggcttgcaa gccatgacta gcaaagaagg ctgctgtccc ttctgtcgca aggattccat
     9481 aaccaatgtt ttggaaacga cgagccaagt tcaaggcttc ttctttggca tcatcagcga
     9541 tggtaaagac gacattacca aaagttggca agtgtagata agaagcttca aaggctttat
     9601 agagagcttt ttccaaagta gcatcagaac ccataacttc acctgttgac ttcatttcag
     9661 gaccgagcaa gctgtctacc ttagctagtt tggtaaagga gaagacaggt gccttgatat
     9721 gaacacgggt gctttcaggg taaagtccat tttggtagcc aagttctgat aaactttgac
     9781 caagaatgag tttggtcgct acttgagcca taggaatatt ggttacctta gataggaatg
     9841 gaacagtacg gctggcacgt ggattgacct caataacgta gactttttca tccttgataa
     9901 caaactggat gttcatcatt ccaaggcagt gaagaccgat tgctaagcgt ttggtgtagt
     9961 ctgcgatggt ctcctgaacc ttttgcgaca aggtttgtgg tgggtaaaca gccattgagt
    10021 cacctgagtg gacaccagca cgttcgatat gctccatgat accaggaatg agtacatttt
    10081 taccatctga aatggcatca acttcgcact cttgcccaac gatataagag tcgacaagaa
    10141 ctgggtggtc tggactagcc ttaacagcag ttcgcatgta agaacgaagg tcttcttcgt
    10201 tttcaacgat ttccatggca cgtccaccaa gtacataaga tgggcggaca agaactggga
    10261 agccaatctt acgagctgca agagctgctt cttcttcatt ggtagccgtt tgtcctggtg
    10321 gctgtggaat atccaattct ttgagagctt gctcgaagag gtcacggtct tcggcacgat
    10381 ctaggtcagc aacctgtgta ccaaggatgg tcacacctgc ttttgccaat ggctccgcaa
    10441 ggttgatggc tgtttgacca ccgaactgaa cgataactcc ctttggttgt tccaagtcaa
    10501 tgacgttcat aacatcttcg aatgtcaatg gctcaaagta aagcttatct gatacagaga
    10561 agtctgttga aacggtctct gggtttgagt tcatgatgat agcttcataa ccagctgcct
    10621 ggatagcctt aacagagtga acggttgcgt agtcaaactc aaccccttga ccgatacgga
    10681 ttggacctga acctaggaca agtacagatt ctttatcaga tctgatagat tcattttccc
    10741 aaccataggt tgaatagaaa tatggcgttt cggagtcgaa ctctgccgca caagtgtcta
    10801 ccatcttata aactggaaca atcttgtttt ccaagcgaag ttggcgaact ttatcatcag
    10861 tcgttcccca gagttcagca atcttacggt ctgaaaaacc attaagtttg gctgttttca
    10921 aaacttctaa atcttgtgga tgagcaccca attcttgctc aatttcaaag atatgcaaga
    10981 gtttatcaag atagaagata tcaatttttg taagctctgc aatttcttca ggtgtgtagc
    11041 cacgacgaat ggcttctgat acgtagaaga gacggtcatc ttgggctttg acaacctttt
    11101 caatcaaggt atcatcagaa actgctgcaa gttcaggtat ttcattgtgg tgcaccccaa
    11161 tttcaaggga gcggcaggcc ttgagaagag attcctcgat gttacgaccg attgccatga
    11221 cttctccagt cgccttcatt tgtgtaccga gacggcgttc acccttttca aacttgtcaa
    11281 atgggaaacg tggaatctta gcaactacgt agtcaagggc tggttcaaac atggcatagg
    11341 ttgaacctgt aactgggttt ataacctcat ccaaggtcaa acctactgca atcttggcag
    11401 ccaacttagc aatcggatat cctgtcgctt tagaagcaag ggctgacgaa cgtgatacac
    11461 gagggtttac ttcgataaca taatacttga agctgttagg atcaagagct agctgaacat
    11521 tacatccacc ttcaatcttg agggcacgaa taatgctcaa gctcgcatca cgaagcattt
    11581 ggttttcata gtctgacatg gtttgcgcag gggcaaatac aatggaatcc cccgtgtgaa
    11641 tcccaactgg gtcaaagttt tccatgttac aaacaaccaa ggcattgtca gctgagtcac
    11701 gcatcacttc gtattcaatt tccttgaaac cggcaatcga acgctcaatc aaacattggg
    11761 taacaggtga caatttcaaa ccattttcag tgatttcacg caattctttc tcgttggcac
    11821 acataccacc accagtacca ccaagggtaa aggctggacg aacgatgact gggtagccaa
    11881 ttgtcgctgc aaaggcaact gcttcttcta ctgtgttaac aatttcagat tctggaatgg
    11941 gttgctcaag ctcttccatc aattgtttaa agagatcacg gtcctccgct tggtcaatgg
    12001 cagataattt ggtacccaga agttcaacgc caagctcgtc taggatacca tttttagata
    12061 attccatggc catgttgaga cctgtctgac caccgagtgt tggtagcaag gcatctggac
    12121 cttccttacg aagaatacgt gtcacaaact caagtgtaat cggttcaatg taaaccttgt
    12181 cagcaatttc cttgtccgtc atgatggttg caggatttga gttaaccaaa acaacctcat
    12241 aaccttcctc tttcaacgac aagcaagcct gagtcccagc gtagtcaaac tcagcagcct
    12301 gaccaataat aatcggacca gaaccaatca ccataatttt ttgaatatca gtacgtttag
    12361 gcatatataa gatattaagg gtgtcaagcg gacaaagcta aaataggagt tatgacgaag
    12421 aactgtcagt tctaggaata actatctttt tagcaccgtc cgtagcccgt attcagttca
    12481 gcaaatacgg agctcccttc tcctttctat tcgtcgcctc tcagggcgac attaaataag
    12541 atacaaagga cgaatagaaa gcgattgaat tttaggaaat caaggaagga ttgacaatcc
    12601 aagttggttt ctctacattc tgagctttcc gtccgtgttc agttacataa attctccgac
    12661 gagcttttac tcgttcttag tttgattgtt taaaaacttc catcatctcg ataaactcgt
    12721 caaataggta gctagcgtcg tgtggcccag gagctgcatc tgggtggtat tgaacagaga
    12781 aagcaggttg gtatctgtgg cgcacacctt ccactgactt gtcattgatt tcttcgtggg
    12841 taataatcaa gtgctctggc aaatcctcgc ggctgactgc ataaccatgg ttctggctgg
    12901 tgaagtctac tcgtcctgtt gcgatttcac gtaccgcatg gttgaatcca cggtggccaa
    12961 acttcatctt ataggtctta gccccgtttg ccattgcaaa gagttggtgt cccatacaaa
    13021 taccaaagat tggaattttt ccttgtacac cgcgaatcat gtcgagtgct tgtggaacgt
    13081 cttctgggtt acctggacca tttgacaaca taactccgtc aggattgaga tggagaattt
    13141 cttcagccgt tgtcgaataa ggaacaactg tcacgttaca gttgcgttta gaaagttcac
    13201 gtaggattga gtgcttgaga ccaaagtcca ctagcaccac gctcaaacca actcctggag
    13261 ctggataaga cgttttagta gaaacctgtt tgatattgtc tgtcggtaaa actgttgctt
    13321 ggagctggtc cgtcacatgg tccatactgt ccccaacatg ggtcaaggtt gcacgcatag
    13381 taccatgctt acggataatc ttggtaagag cacgcgtatc aattcctgaa atccctggaa
    13441 ttttcttggc tttcaaaaat tcatccaagg tcatttggtt gcgccagttg ctagctctac
    13501 gcgcttcttc aaaaacaacg actcccttac aagttggaat aatggattca taatcatcac
    13561 gattaatacc ataatttcct accaaaggat aagtaaaggt caagatttgt ccattataag
    13621 actggtctgt aatggattct tggtagccgg tcatccctgt attaaagacg atttcgcctg
    13681 ttacatcaat atctgctccg aaggccttgc cttcaaaaac tgtgccatct tctaatacta
    13741 gaattctttt tgtcatattt tcacctctcg tggacgctca ctggcgtctt ttaacgtctt
    13801 gtgttttagt tggcgtttct actcgctagt acggattcta agattgccat tcgaacaaag
    13861 acaccattgg tcatttgttg gacaatccgt gattttggtg cttcaaccaa gtggtctgct
    13921 atttctacat cacgattgat tggagctggg tgcatgagga ttgctgtttc tttcaaacga
    13981 tcgtaacgtt cttgagtcaa gccatgttgg gcatggtagt cttcttttga aaatacagct
    14041 ccactatcat ggcgttcgtg ttgcacacgg agaaacatca tgacatcaac ctgatcaatg
    14101 atttcatcaa tggttacaaa ctgtccatag tctgcaaact cttgacttct ccattcctca
    14161 ggtccagcga aaaagagttc agatcccaag cgtttcaaaa tctgcatatt ggatttggca
    14221 acgcgtgagt ggtccaagtc acctgcaata gcaactttaa gaccctcaaa gtggccaaat
    14281 tcctcataaa tggtcatcaa atcaagcaag ctctggctag ggtgttggcc cgaaccatct
    14341 ccaccattga tgatggaagt cgtaatcgtt ggactagcaa tcaattctct atagtagtcg
    14401 acctctggat ggcgaatcac acagacatcc actcctaaag cagacagagt caaaatggtg
    14461 tcataaagtg tctcaccctt attaaccgaa ctagtcttca catcaaagtc aagtcgttcc
    14521 aatccaagtt taatctctgc gacttcaaag gacttatgtg tccgtgtaga atcctcaaag
    14581 aagagattgg aaacaatcgg atggtcttca tagggaagct gggctccatt tttaaactca
    14641 attcctcgct tgatcaattt cattacttga tcgacagtga ggtcttccat ggacaccaca
    14701 tggttcaatg cttgttgatt ttctgacatg gctactcctt taactttcta agcttcttca
    14761 gtaatcagaa ctctgtcttg gtcatcaagt tctgtcatct ctacgatgat ttcttcagaa
    14821 cgactggttg ggatattttt tccaacgtaa tctggacgga ttggcaattc tctatgtcca
    14881 cgatcgacta gaactgctaa actcacacgc gcaggacgac catgaccgac aatattatca
    14941 atagcagcac ggatggtacg acctgtatag agcacatcat ccaccaagat aacttcgcgg
    15001 tctgtcacat cgacagaaac caaagaagta tcttctccac ttttaacatc atcacggaaa
    15061 ggtttagtat ccaattccac aacaggaact gaaagatttt ctaactgctt caaacgttct
    15121 tggattcggt gggcaataaa gacaccacga gttttaatac cagccaagac gatcttattc
    15181 aaatctttgt tgcgttcgat aatctcataa gtaatacgcg taatcgctcg tttgacggtc
    15241 aattcgtcta caacttcttt tgttttcatg acaaacctcc aaaaagaaaa gtctccttaa
    15301 acaaggagac ttgaaattta tagccaagcg agccctactg cacacagtat agacttcacc
    15361 cttctacttt atcgcgctcc ttgcctgcct cacgggacag gtttaaagga atatttagtt
    15421 atcatttact atagcacaaa gcatgcttaa aatcaagcaa aaagtttcaa tgtagcatct
    15481 tacaaattgc taaaatcata taattgtggg tactggtcac actctggatt ttttggatgg
    15541 caaatggctc ttccaaaata aatcatggcc tgatgggcag ctaaccactg ctcaggcggc
    15601 aagatatcca tgacccgctt ttccacctca agtggcgtcg ctgatttttt gacaatatcg
    15661 tggtgtttgc aaatacgctc cacatgagta tccactgcaa aggctggaat cccaaatcct
    15721 acactcatga caacattggc tgtcttgcga ccaacacctg ccaaactctc caattcttca
    15781 cgtgtctgag ggacttgacc atcaaaatcg tctagtaact gttgggcaca ttttttaagg
    15841 aatttagctt tattccgata caatcccaag cgagaaatat gtgaagcaat ctcactctct
    15901 gtcgctacag acatagcttg gggtgttgga aaggcaacaa agagacctgg tgtggcctta
    15961 tttaccgctg catctgtcgt ctgggctgat aacatgaccg caaccaggag ttcaaaatga
    16021 ttggtaaaat caagactagg cttggcatct gggaagaggg caatgatttc ttctagcacc
    16081 tttcgtgctc gtttttttga caagaccatt attcatctcc gtcaaatagt ccttgtaagc
    16141 cagcaaaagg actgttttct tctttcttta ctgcttgttg agcttggtat tcttcctctg
    16201 tcatgatttg ccagtcattt cctgagacaa atccttgacc agcttcttct tcagccgtca
    16261 agaccttgat aggaatgttt agcaagatat tgtctgatac actctcagca aggtcaagcg
    16321 cctcattttc gatgggcaag accaagtcat catctaaaat ttcttgattt agatggttag
    16381 ttgcgccttc catgaaaact tccgtgactg gataagattc aactaactca actggctcca
    16441 tactgcgact cgaagcaaga acaatggtat aagatagttg ataatctaag aaatacatac
    16501 ggtcttcata ttgtactttc ccaactgcaa ggatatcttt tacatctaaa atttcttgat
    16561 tacgtgcacg caggtcatca actaaatcta acgtttgttc aaagttcaaa ccttcagact
    16621 gcttacgaat ttcttgaata tttaatttca tacttcctcc ataaagattt actctcttga
    16681 ttataccatg aaaaggctac aaatcagcac accaaacttt gtaattaaaa ttcagaattt
    16741 taacatattt aatatgacag ttttgttttt tagtgctata ctatagggaa agagtacatc
    16801 agatcaagga ggatgctcac atggaagaca agaaactcat tcaactccta tccaagttaa
    16861 ataaaagcta ccaaaactgt aaacagggta cggcagatga tattcgacta caagagctgc
    16921 taaacactac tatgcaagag ctcaaaaaaa cggaaaagtt gaacaacagt atcttaattg
    16981 atcttgagaa attttaccaa cccaccagtc ttctgattgg actgggtagc ctaaaactaa
    17041 acgatcaagc acgcactgct tggcgaaact atgataaatt ccattacgag catatcaaac
    17101 acgtactaag tctctatgga cctgtttttg gattttagag catagaattt ccagttttct
    17161 gttgacaaaa tttccttaaa ggtataatat aaagatacta atactcggag gtaagggaga
    17221 catgaacaac taagtctatc aaataaagaa cctttattta gtagatcttg tttttgtctc
    17281 tttttgtgtg ctcttttatg ctctttttct ggcatgttaa tagagttttt ttgacataga
    17341 ctttgggctc tactaggtaa agtagagctt tttgttatgc actatgaaca ttctagaaag
    17401 ggaaatcata tgataaaaat caatcatcta accatcacac aaaacaaaga tttacgagat
    17461 cttgtatctg acctaagcat gaccatccaa gacggggaaa aggttgctat tattggtgaa
    17521 gaaggaaatg gcaaatcaac cttacttaaa attttaatgg gggaagcttt gtctgatttc
    17581 actatcaagg gaaacatcca atctgactat cagtcactgg cctacattcc tcaaaaactt
    17641 cccgaggagc tgaaaaagaa aactttacac gactacttct ttttagattc tattgattta
    17701 gactacagta tcctctatcg tttggcggag gaattgcatt ttgatagcaa tcgtttcgca
    17761 agtgaccaaa agattggcaa tctatcaggg ggagaagctt tgaaaattca gcttatccat
    17821 gagttagcca aaccctttga gattctattt ttagatgaac cttcaaatga cctagacctt
    17881 gagacagttg attggctaaa aggccagatt caaaagacca ggcaaaccgt tattttcatt
    17941 tcccatgatg aagactttct ttctgaaaca gcagacacta ttgttcactt gcgactggtc
    18001 aaacaccgta aagaagcgga aacgctagta gagcatttag actatgatag ctatagtgag
    18061 cagagaaagg ctaattttgc caaacaaagt cagcaagctg ctaacaacca aagaacctac
    18121 gataaaacca tggaaaaaca tcggagagtt aagcaaaatg tagaaactgc gcttcgagct
    18181 accaaagata gtactgccgg tcgcctattg gctaaaaaga tgaaaactgt cctctcacaa
    18241 gaaaaacgct acgaaaaggc agctcagtcc atgactcaaa agccacttga agaggaacaa
    18301 atccaacttt tcttttcaga catccaacca ttaccagctt ctaaagtctt agtccaactg
    18361 gaaaaagaaa atttgtccat tgacgaccga gttttggttc aaaaactaca actaactgtc
    18421 cgtgggcaag aaaaaatcgg tattatcggg ccaaatggtg ttgggaaatc aactctgtta
    18481 gccaaattaa agcaacttct gaatgataaa agagagattt cccttggttt tatgccacaa
    18541 gattaccaca aaaaactgca attggattta tcaccaatag cttatatcag caaaactggg
    18601 gaaaaagagg aacttcagaa aatccaatct cacctagcca gtctcaattt cagttatcca
    18661 gaaatgcagc atcaaattcg ctccttatct ggcggacaac agggaaaact cctgcttttg
    18721 gatttagtcc tgcgcaaacc aaactttctc ctgctggatg aacccacacg aaacttttct
    18781 cccacttctc aaccccaaat cagaaaactc tttgcaacct atccaggcgg tctcatcact
    18841 gtttcgcatg accgtcgttt cttaaaagaa atctgctcga tcatctatcg catgacagaa
    18901 cacggtttga agctagttaa tttagaagat ttataaattt gcaacatagc agataggtgg
    18961 ggcaaaaaca caattttagg agaaaaagaa gtaaatcttc ccatagtaaa acgcataata
    19021 tcaagttttc acacctgata ttatgcgttt ttcagatttt aaagactttt ttccagcctt
    19081 cattttacat ctgttttaaa cgttcaatcc gttctgagat aggtgggtgg gtataaaaga
    19141 gtttttggaa ccccccacct ttcttgggat cattgatata aagagcactg ctagcatcat
    19201 cgacgtggcg actcataggt ttgctattgt ccaacttatc tagggcatta atcattccct
    19261 ggggattgcg agtcagctcg acactagatg catctgccag aaattccctc tgacgagaaa
    19321 tagcgagctg aaccaaggtt gcagcgagag gtgccagtac aatagctagt agggaaacca
    19381 ctagcataat gatttcaaga ccatttccat ctcggtcatc atcacttcgt ctgcgacctg
    19441 ctccacccca ccacatcata cgacctgcca taccagaaag catggtgata gcactaacaa
    19501 gggcaactgc aatagtcgaa atacggatat cataattacg aatatgactg acttcatgtc
    19561 ccataacagc ttctagttct tcacgattca tgatagctag tagacctgaa gtcgcagcaa
    19621 cagccgcatt ttgaggatta gaacctgtcg caaaggcatt taaggctgga tcatcaatga
    19681 tgaaaacacg gggcatagga atctgagcga ccagagccat atcttccact acatggtaga
    19741 ggtctggtgc cgtttgctca tccacctcac gcgctccatt catggacatg acaatctctg
    19801 tcgattgaaa aatcatagac aaagcgtaga taaagccgat aatcagtgcg ataaccaaac
    19861 caccaagtcc agatcttata aagagataac caaccgcata accaacaaga gctaagagta
    19921 ggaaaaatac caccaacaaa atccaggttt ttcgtttatt gcttgcaatt tgatcaaaca
    19981 acatcttagt cacctaaacc gctaaaatca actttaggaa ccgacttttc ctcttcaggt
    20041 gtttgaagga aatctgccgc tttaaatcca aacattccag cgataatatt gctcgggaaa
    20101 gtttctaatt ttatattgta gttgctgaca acactgttat agagttgacg agagtaagaa
    20161 attttatttt ctgtgtttgt caactcctct tgcaatttaa caaagttagc actagctttc
    20221 aaatctggat agctttctgc aactgcaaaa atacctgaaa cctgacgagt gagggcatca
    20281 ctggctttca tagcttctgc tggtgaagtc gctgccgcca cttggttacg tagttctgcc
    20341 accttttcaa gggtagaacc ttcatatttg gcataacctt ttacagtctc aatcaagttt
    20401 ggcaagaggt catttcgacg tttcaactga acatcaatct gactccaagc ctccttggtt
    20461 tgcatacgat ttttaaccaa accgttatag ctaacaatca caaaaataac aataagagcg
    20521 ataactccaa gaataatcca agtcataata taagtccttt ctgcttttag attagtacca
    20581 gtatatcaaa ttttctatga ttgtggtaaa ataagatgat actaaagaag gaaataacta
    20641 tgaaaccaaa aacattttac aacttgcttg ccgagcagaa tcttccactt tcggaccagc
    20701 aaaaagaaca atttgaacgt tattttgagc tcttggtcga gtggaatgag aagattaatt
    20761 tgacggcgat tacggacaag gaagaagttt atctcaaaca tttttacgat tcgattgcac
    20821 ccattcttca aggtttgatt cccaatgaaa ctatcaaact tcttgatatc ggggctgggg
    20881 caggatttcc tagtctacca atgaaaattc tctatccgga gttagatgtg accattattg
    20941 attcactcaa taagcgcatc aacttcctac aactcttggc tcaagaactg gatttgaacg
    21001 gagttcattt ctaccacgga cgtgccgaag attttgccca agacaagaac ttccgtgctc
    21061 aatatgattt tgtaacagct cgtgcggttg cccgtatgca ggtcctatct gaattgacta
    21121 ttccctacct taaggttggt ggcaaactat tagcactcaa ggctagcaat gcgcctgagg
    21181 aattattaga agctaagaat gccctcaatc tcctttttag taaggtcgaa gacaatctca
    21241 gctacgccct accgaataga gatccgcgct atatcacagt ggtagaaaag aaaaaagaaa
    21301 caccaaataa atatccacgt aaggctggta tgccaaataa acgcccactt taaatttttt
    21361 agtaaacaaa tgtttactaa atcagcctcg ctcttttatt tctaggcttg ggaaaaaatg
    21421 attt
//
LOCUS       DBERMR010000037        21314 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.037, whole genome shotgun sequence.
ACCESSION   DBERMR010000037 DBERMR010000000
VERSION     DBERMR010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 21314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21314
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.037"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(300..518)
                     /locus_tag="ACL8A1_001477"
     CDS             complement(300..518)
                     /locus_tag="ACL8A1_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS1202231.1"
                     /translation="MALNYKPLWIQLAKKGLKKTDVIAMAGLTTNVMAQMGKDKPITF
                     KNLERICKALSCTPNDIISFEDNFSDEE"
     gene            complement(764..1042)
                     /locus_tag="ACL8A1_001478"
     CDS             complement(764..1042)
                     /locus_tag="ACL8A1_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202232.1"
                     /translation="MKKDKEVYDKVCEYLSPHLNKDMEVLELACWFRVITVIEANSYV
                     NIRSSRLLPKFKTQKINSWCAAYNIHFNNGYCKQHTNEFKDSLKSLIL"
     gene            complement(1256..2887)
                     /gene="rlmD"
                     /locus_tag="ACL8A1_001479"
     CDS             complement(1256..2887)
                     /gene="rlmD"
                     /locus_tag="ACL8A1_001479"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HGS1202233.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHDLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(2961..3935)
                     /locus_tag="ACL8A1_001480"
     CDS             complement(2961..3935)
                     /locus_tag="ACL8A1_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HGS1202234.1"
                     /translation="MRKKLFLTSAAVLWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDVRKIVENTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(4009..4623)
                     /gene="pmp23"
                     /locus_tag="ACL8A1_001481"
     CDS             complement(4009..4623)
                     /gene="pmp23"
                     /locus_tag="ACL8A1_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HGS1202235.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVQLNLYIIKCFTLFSTSG"
     gene            complement(4623..5597)
                     /locus_tag="ACL8A1_001482"
     CDS             complement(4623..5597)
                     /locus_tag="ACL8A1_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HGS1202236.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSSKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDVSRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(5605..6861)
                     /gene="glyA"
                     /locus_tag="ACL8A1_001483"
     CDS             complement(5605..6861)
                     /gene="glyA"
                     /locus_tag="ACL8A1_001483"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HGS1202237.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYESLSPFKTSGIRIGAAAITARGFGEEESRKVAEFIIKTLKN
                     SENEAVLEEVRSAVKELTDAFLLYED"
     gene            complement(6923..7354)
                     /locus_tag="ACL8A1_001484"
     CDS             complement(6923..7354)
                     /locus_tag="ACL8A1_001484"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1202238.1"
                     /translation="MLRDLQETDVKAICDINQETLGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEQEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRCIRIF"
     gene            complement(7356..7958)
                     /locus_tag="ACL8A1_001485"
     CDS             complement(7356..7958)
                     /locus_tag="ACL8A1_001485"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HGS1202239.1"
                     /translation="MMDRIRQELEKGGAVILPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNVASLEDILHFSKNQPAYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(7942..8781)
                     /gene="prmC"
                     /locus_tag="ACL8A1_001486"
     CDS             complement(7942..8781)
                     /gene="prmC"
                     /locus_tag="ACL8A1_001486"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HGS1202240.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASDQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKKVRTLKDQFGQDRMVVVDD
                     GQD"
     gene            complement(8781..9860)
                     /gene="prfA"
                     /locus_tag="ACL8A1_001487"
     CDS             complement(8781..9860)
                     /gene="prfA"
                     /locus_tag="ACL8A1_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HGS1202241.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEELAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(9857..10447)
                     /locus_tag="ACL8A1_001488"
     CDS             complement(9857..10447)
                     /locus_tag="ACL8A1_001488"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HGS1202242.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRGLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            10570..10752
                     /locus_tag="ACL8A1_001489"
     CDS             10570..10752
                     /locus_tag="ACL8A1_001489"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HGS1202243.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            10889..12262
                     /gene="mnmE"
                     /locus_tag="ACL8A1_001490"
     CDS             10889..12262
                     /gene="mnmE"
                     /locus_tag="ACL8A1_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HGS1202244.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGVRLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNIRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEANLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLIEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(12309..13244)
                     /gene="dapA"
                     /locus_tag="ACL8A1_001491"
     CDS             complement(12309..13244)
                     /gene="dapA"
                     /locus_tag="ACL8A1_001491"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HGS1202245.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKTIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(13300..14376)
                     /locus_tag="ACL8A1_001492"
     CDS             complement(13300..14376)
                     /locus_tag="ACL8A1_001492"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HGS1202246.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGGSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIASF
                     PGAVLEDDVVHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(14962..15034)
                     /locus_tag="ACL8A1_001493"
     tRNA            complement(14962..15034)
                     /locus_tag="ACL8A1_001493"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14999..15001),aa:Thr,seq:ggt)
     gene            complement(15134..15643)
                     /locus_tag="ACL8A1_001494"
     CDS             complement(15134..15643)
                     /locus_tag="ACL8A1_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HGS1202247.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(15641..15737)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            15766..16197
                     /locus_tag="ACL8A1_001495"
     CDS             15766..16197
                     /locus_tag="ACL8A1_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HGS1202248.1"
                     /translation="MKKPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            16279..16656
                     /gene="mscL"
                     /locus_tag="ACL8A1_001496"
     CDS             16279..16656
                     /gene="mscL"
                     /locus_tag="ACL8A1_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HGS1202249.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(16698..17792)
                     /gene="hemH"
                     /locus_tag="ACL8A1_001497"
     CDS             complement(16698..17792)
                     /gene="hemH"
                     /locus_tag="ACL8A1_001497"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HGS1202250.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            17947..19167
                     /gene="pepT"
                     /locus_tag="ACL8A1_001498"
     CDS             17947..19167
                     /gene="pepT"
                     /locus_tag="ACL8A1_001498"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HGS1202251.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEA
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSNRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVRSL"
     gene            complement(19210..19863)
                     /locus_tag="ACL8A1_001499"
                     /pseudo
     CDS             complement(19210..19863)
                     /locus_tag="ACL8A1_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672614.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family hydrolase"
     gene            19861..20025
                     /locus_tag="ACL8A1_001500"
                     /pseudo
     CDS             19861..20025
                     /locus_tag="ACL8A1_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057566.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(20296..21308)
                     /locus_tag="ACL8A1_001501"
                     /pseudo
     CDS             complement(20296..21308)
                     /locus_tag="ACL8A1_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1,RefSeq:WP_007521835.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
BASE COUNT         6146 a         4452 c         3956 g         6760 t
ORIGIN      
        1 aaaaaaggat ctagttttta tttttagtta aattatgtct atatttttca ttaactcatc
       61 aataaaaatt tgtttgctaa tatctctttc ttctgatttt gtttcaagga ttattgctac
      121 atcattcata tttttaggta aataccaacc atctggctta tctgaatatc ccttgaagcc
      181 taattgatta aaagtagtta tttgaccagt accttgattg atgttttctt caacttcatt
      241 aaagcctata cttctcttgc ataatcccta acttgatctt ctgtccttaa agtcattttc
      301 tattcctcgt cactaaaatt atcttcaaaa ctaataatat cattaggagt gcaagataaa
      361 gccttacata ttctttctaa attcttaaat gtaattggtt tatcctttcc catttgtgcc
      421 ataacatttg ttgtaagtcc tgccatagct attacatctg ttttctttag tccttttttt
      481 gctaactgta tccataatgg tttatagtta agtgccataa tatccctctt tttttcttca
      541 ttagatttat ttaattatag cataaatatt gatttcattc aatcttacat tgatttagtg
      601 atttgtttta gagaatttgt tgtgttattc cgtccttatt ttgccataaa ttgttttaag
      661 tgggacctct tatccttacc atttttacct cctaattttg agtataaaaa agacgataga
      721 gtttttaatt tctatcgtct gagatttaca tattaaattt tcatcacaaa ataagactct
      781 ttaaagaatc tttaaattca ttggtatgct gcttacaata tccattatta aaatggatat
      841 tgtaagcagc acaccaacta tttatttttt gagttttaaa ctttggtaga agtcttgaac
      901 tccttatatt tacataacta tttgcctcta taactgttat gacacgaaac caacaagcaa
      961 gttcaagcac ctccatatct ttattcaaat gaggactaag atattcacaa actttatcat
     1021 aaacctcttt atcttttttc ataaaagggg catacaactt agcaaattta tcctaaaatt
     1081 ttttgttgcc tttatcaatc atttcaacgc tcctttaaat ataaaaatga tcctatcaat
     1141 ctttatattg agcctatttt ctaattgata ccttgcaaga aaggaataat agctgttcct
     1201 ataccaagta ggaatgtaat aaatgcagaa agtcatatac tgtcattctt ttctattaaa
     1261 tcattttgaa gtgtctcaaa gcatccatga tggcttcttc tttttcaggt gtacactgtg
     1321 gaataatttg tttatccttt ttagacttgt tgtaatgttc tcgtaattct attccacatt
     1381 tcttttttat ctgtgcaata tataatgtcg aaacttttaa ttcaaattta ttccaaacat
     1441 attctttgat ttgagcatat gttgctttgc tctccgcact tgtcaaatcc atctcatcca
     1501 gctcaatttc aacacttatg tgcttatcga catcgagttt ggacaaaagt gctaccgtct
     1561 cgacgtgatg cgtttgagga aatagatcca ccggctggac tttcttcaat tcatatccca
     1621 attcttggta gagtttgata tcacgcgcca tggttgcgac attgcaggag atataggcga
     1681 tgcgatcggc tcctgtttgg gcgcttgctt tgataaagct ttctgtcaag cccttgcgtg
     1741 gaggatcaac caagataacg gttggttgaa tcccatcttt aagccaattc ttcatagcat
     1801 tttcagctgt gtcacagaca tagtgggcgt ttgaaatatt gttcagctga gcattttttt
     1861 tactattctc aaccgcttct ggaatcagtt caacaccgta gacttctttg acatgcttgg
     1921 cgactgataa accaatggtt ccaataccag aataagcatc aataaccacg tcatcttttt
     1981 ttaactctgc aaagtcaatg gctgtttgat agagtttctc cgccatttca gtattgactt
     2041 ggtaaaaggc tgggccagcg atttggaagt catttcccaa catctggtcc gtaatatagt
     2101 cttgaccata aagagtgcgc cactccttac caaaaatcgc attggtattc tggtcgttga
     2161 tattttgcat gacagacaca atctctggga actgcttgat aacttgttca atcaattggt
     2221 caacacgaaa aacttttgga cgagttgtca ccaaaacgac catgatttgt cctgaatagt
     2281 gaccacgacg caccacaaga ttccgaatca atccagactg ttccttttcg tcataaggtt
     2341 ttaaatcaaa acgacggagc aggtctcgaa gagctactac gacttggtca atgacaggat
     2401 cctggataaa gaaatcttca aggggcatga ggtcgtgcga attcttacgg aaaaatcctg
     2461 tttccaagac accattcact cgacgaacgg gcacctgcgc cttattgcga tacttgactg
     2521 gatgttccat accaagcgtt tcagcaactt ctacatctgc aattccagca atcttgtaga
     2581 gactgtcctt gacttgcttg gttttaaact tgagctgttc tggataagaa aggtgtccta
     2641 aatccgcgat tcctgaacgc aggtaagcca aatctagatc ttgattacgg tgtggtgact
     2701 ggacaaggta tttttcaact tttccaaagc caatcttttt attgaccttg aggacacgca
     2761 tgagaatttt ttcactcggt aaagcattct ctacaaaaaa gaccaaacca tctaccttgg
     2821 caactcctgc cccttcatgg gtcaaatcaa caatttcaac ttctacaata tcatttttct
     2881 ttaacattct tttctctttc tattggccga caaaaaagac ggcaacgtta cggttaccat
     2941 ccattatacc atgaaatttc ttaagaacca aaactcttga agaagttgac aagggcttgc
     3001 cagagggagc ttaagaagtt accaccgtcc tctagcgcct tatccgcatc aaagttaagg
     3061 ttgatatttt taaaactgtc gccagcttgt gatacgatgc tttgtttaag gtcatttagg
     3121 gttttagtga aatctgcatt gctgaggata tcactctttg agagattcaa ggcaaaattg
     3181 atgatgatat tgatctggtt tcctgttatg acctgatcaa gtttgtaatt ttttaaggta
     3241 ttttcaacaa tcttgcgtac atcttcttct gtcagatttc ccttgctttc tttagctttg
     3301 gcgagtcctg acttgatatc agctagggca acgtttaatt tattagcatc atagcctgat
     3361 ttgtccttgt tttcagcatt gatatctgac aaagccttta gctcttcttg agccaaatct
     3421 ttattggctt gcggcacctt ggctccatta gcttctagcg aatagtagat cccagccaaa
     3481 gcactctcac ctgtaactgg aataggggct gctacagtga ttttggcatg ttccataccc
     3541 agcgttactg ctgcgtttcg gtacatatcc tgagtcacct tagtgatatt ttctggtgtt
     3601 tcaatcttga cctcaagtgg cgatttgtca cctagctttt gaatcttggc tgatgaatac
     3661 aactgtaagc tagagtcatt ggccacattc atgattttag aataaacatc aggtgtcatg
     3721 gtcttgagtt ctttggtatc tgttgaggca ttgtagccca gttttttaag agtttgattt
     3781 ttttggtctt cagataggga ggaacctagg acatattcag gttggacata ggtttcatcg
     3841 ataacttttt gaacatctgt tgctgcatgg acgctattca tagctgttac tgcccacaag
     3901 accgcagcac tagtcagaaa gagtttcttt ctcataggga atttcctcct ttacctttct
     3961 agagtaatat atctatctta aagaaaactt ataacaaaaa cacctgaact agccagatgt
     4021 tgaaaagaga gtgaaacatt tgatgatgta aaggttgagt tgcacctgtc tagaataata
     4081 atagtttcct ccatttacat agagttcggc accgtgaaaa atggaaatgg gatgaatata
     4141 actataagtc tttccagtcg tattaccaag caagggggca acagtctcac gagagtactg
     4201 tttggctaga gccaaggtat tttccttgcc attttgggcg ataaaatcga tataggcagg
     4261 tccaaaatta taggcttgaa cagctgtcca gatatctaca cccttcttct gcgccagata
     4321 gagattgcct gtcagagttt gaatgccttg ccgaatgcta gaggcattat cattgatggt
     4381 gttggtggaa ccacttgcag actcactaga ctgcataaca tcgccttctt ttccttttgt
     4441 ttcagtataa atcatagcaa gcacaagctc ttcgtttgct ggggtgtctt tttcactcaa
     4501 tatttctcgc accatgggtt gataggtcat gacttgtttg acatcttgat gaacgcggta
     4561 agctttatag ccagcaaaaa ggaagactgc tagtacaagc actcttcgaa ttcgtttaaa
     4621 cattatttac tttggatatc ctcgatattt ttgattaaga tagagtaggt tccattttcg
     4681 ttttggataa actcaacaga ctcggcgtct tgatagacgt tattgggaac gatgagctca
     4741 attccatttg ataaggagag tttttggttt tcaaatttct ttaattggcg actgacatca
     4801 atttcatcaa attgaacagg ttctggtacg gcttctttga cttggtcaat aaagctcaaa
     4861 cgagccgtca gattgttgtc aaaaaggtca ttagccaatt tctcaggtga caattcattg
     4921 ctttcttcta ggttgttgaa aatagctgat ttgaccttgg attgaaattg aaaatcatct
     4981 gtgttaaaag attcagcaat tctctgggct gttttttcca gttccttgat agatttttta
     5041 ggagaaatct taggagcgac agcaagaaga ttatctgaaa aatagttcaa aaaagtcccg
     5101 ttgtacttga ttcgtttttc aatcaggtga tacttgctac tctgaagatt gaccaccaag
     5161 gcctcatcag ctcctgttcc aaatccaggc aggttattct gagttagctt gattggatta
     5221 tcaacttctc ctccgaggtg ggtcaaggtc tcccgcaggg caattcgcaa gaaagcgaaa
     5281 tgttctacac cttctttaga aaattgaaca aaaatcaagt cattggtctt gagattttca
     5341 gaaatgctaa actcctcttt ccagagatta gccagcgtta ctgatgtctc caacaaatcg
     5401 tctgtaatat gattgaagaa gggattttct tcttcgaaaa tcccagtctt ggcttcatct
     5461 gaatacacat gttcaatttt tttacgcagg tattcttcga tttttggagt aatattgaga
     5521 aacttatctg ctaagaacag ttcggtatca tccggactga actggtgaat aatggctttc
     5581 ttaatataaa tgtccataaa agttttagtc ctcgtatagt aggaaggcat ctgtcaattc
     5641 tttgactgca cttctcactt cttctaatac agcctcattt tctgaattct taagggtttt
     5701 aatgatgaat tcagccactt tgcgactttc ttcttcacca aatccacgtg cagtaatggc
     5761 tgctgctccg atacgaatcc cacttgtctt gaatggtgac aagctttcgt aagggattga
     5821 gtttttattt aaggtaatat tgacttcatc caacaagttt tgagcaactt tgccgttttc
     5881 tacaacttta gtcacatcaa caaggaagag atggttttca gttccacctg aaataatacg
     5941 gaaatcaggg tcttgcaaga agacatctgc catagccttg ctgttcttaa ttacattggc
     6001 agcatattcc ttgaaggctg gatccaaaac ttctttgaag gaaactgcct tagccgccac
     6061 aacatgctct aaaggaccgc cctgaatacc tgggaaaata gctgaattga tttttttagc
     6121 aagttcttcg tcattggtca aaatcaaacc accacgagga ccacgaaggg ttttgtgggt
     6181 cgttgttgtt gtgatatgag cgtatggaac tgggcttgga tgaaggccag ccgcaaccaa
     6241 accagcgata tgggccatgt ccaccatgag cttcgcccca acagcatctg caatttcacg
     6301 gaattttgaa aaatcgataa tttgagaata ggctgaagca ccagctacaa tcagttttgg
     6361 ttttacttct tgggcttgtt tcaagatagc atcaaagtct aagagttccg tttcaggatc
     6421 cacactataa gaaacaaagt tgtaggtttg accagagaag ctaacaggag ccccatgagt
     6481 caaatgacca cctgatgcca aatccattcc cataaccgta tcacctggct caatcaagga
     6541 catgtaagcc gcacagttag cttggcttcc tgaatgtggt tggacattgg caaatttagc
     6601 accgaaaatt tcttttgcgc gttcaatagc aagagtctct acaacgtcta ctacatcagt
     6661 tccaccataa taacggcgtc ctgggtaacc ctcggcatat ttatttgtca agatagaccc
     6721 ttgagctgcc ataacagcct tggaaactac gttttccgaa gcaattaact cgatattatt
     6781 ttgttggcgt tcttcttctt tggcaatagc attccagaga tcagcatcat atgctttaaa
     6841 atcatctttg tcaaaaatca taggtcttct cctttattgt gtgactagtc cattagtttg
     6901 attttacaat aagaaaatca aactaaaaga tgcgaataca ccgtttctgc attttatcac
     6961 aagtatagcc aactttttca taaaatgcat gagcacccag acgatgattg gcagaattta
     7021 agcggataaa cccataacca catcttttgg cttcttgttc caacccttgt agtaaacttt
     7081 taccgatacc ttgaccttgc gcttgaggtg aaactgctaa agctaagata ttaaatcctg
     7141 ctttggaata gagtgattcg taaacttcag cgtggacata tccaagtaag acatgattag
     7201 ctgcatcctc atagccaagt aggaaatgat gggaatcctg agacagtcta gctagttggc
     7261 tagccgtttc ctctggacta aaagtataac ccaaagtctc ttggttgatg tcacatatcg
     7321 ctttcacatc tgtttcttgc aaatctctta gcatttcaag cctcctcaaa agaaatctct
     7381 ggcaaccgag caagaatatc ttctcgttta attgcgcctt ggcgtaagat tttcaccttg
     7441 tctccagaca aatccacaat agttgaatcc tgtccagtta gaaaagcatc gtcttccaga
     7501 cccagaacct cttggtcaaa atccttcaga atttgttcaa aggttacacc acttgcctga
     7561 cctgagatat tggcagacgg cccaatcaag ggacctgtct ctcgaattaa atccagtgtg
     7621 atagggtgac tgggcatccg aaatccaata gttgcaaggt cagaatttac ccaataggga
     7681 actcggtcat tggcttcgag aataatggtc aagggacctg gcaaaaaggt ctctacaagt
     7741 ttttgtagat aagctggctg attcttagaa aagtgcaaga tatcctctaa agaggcaaca
     7801 ttgagattga gcgccttgtc tctaggacga cgtttgagct ggtaaacatg gtcaactgct
     7861 ttttcgtcta aggccttggc aaaaagacca taaaccgtct ctgtaggcag aatgacagct
     7921 ccaccctttt ccaactcttg tctaatcctg tccatcatca accacgacca tcctatcttg
     7981 accaaattgg tctttgagtg ttcgaacttt tttttcagga agatgttttc taaaaagttc
     8041 gggaacactt tgaccttgct tgtatccaat ttcaaggtaa atcttaccac catccttgag
     8101 atagtctttt gcatcttccg caattctaca gtaaatagct aggccatcct cgtctgcaaa
     8161 gagagctaga tgaggctccg aatgcaaaac attcaagcct acctctgact catcttcacg
     8221 agagatatag ggtggattgg atacaattat atcatatttt tcagaaattt ctgcaaaaca
     8281 atcagatttt ttaaaaaata tattaagatt ttgatcgcta gcattctctg atgcaagctc
     8341 taaggcctct tgagaaacat ctgctgctgt cactgaccaa tctggtctat tttttgctag
     8401 agcgagagca atagctccac tacctgttcc aatatccaga actgagagat tcgtctcaag
     8461 attttcagtt agaataagct ccactaactc ctctgtttct ggacgaggaa tcaaaacccg
     8521 ttcatccacc tttaaatgca ttccataaaa atctgcctgt ccaatgatgt actgagctgg
     8581 cttgtgagct gctagttgct ggtaaatatc ttctacaaat tgtttttctt cctctgttgt
     8641 cacctcctgc tggagggcaa aaataaagtc tgtaaaagat agatttttca gactacgata
     8701 gacaaaagag aggctttccg cttcctctcc ttgtcttatc aactcttctt caaaatttga
     8761 aaataattga gctaatttca ttatttgttt aattcttcta gtttttgtgt ttggtcataa
     8821 agcaccaagg catccacaac ttcgtccaat ttaccagaca aaatcgtatc tagtttttgg
     8881 agggtcaagc cgatacggtg gtctgtgaca cggttttgtg ggaagttata agttcggatc
     8941 cgttctgaac ggtcaccagt accgattgtc gacttacgct cagcgtcttg ttcatcctga
     9001 gcaatctgag caaagtggtc agcgacgcgt gcacggataa tcttcatagc cttctcgcgg
     9061 ttcttctgct gggtacgttc ttcctgcatc tcaaccttga tattggttgg caagtgaacg
     9121 atacgaacgg cagtcgcaac cttattgacg ttctgtccac cagcaccaga ggcgtgatag
     9181 atgtcgacac gaaggtcttt tggatcaatg tcgtattcaa cctcttcaac ttctggcata
     9241 acaagaactg tcgctgtcga agtatgaaca cggccttggc tttctgtcac aggaacacgt
     9301 tgcacacggt gggcacctga ttcatactta agcttagagt atacagactg acctgaaacc
     9361 atagcaacca cttctttaaa accaccgaca ccattcatag aggcttccat gacttcaaag
     9421 cgccaacctt gggcttccgc atacttttgg tacatagtta gcaaatctcc agcgaaaagt
     9481 gccgcttcgt ctccaccagc tgctccacgg atttcaagga tgatattctt gtcatcgttt
     9541 ggatcctttg gaaggagcaa aattttcagt ttttcttcat attcttcttt ttcagccttg
     9601 gcatctttga gttcttgctt ggccaattct tccaagtccg catctccgcc tgattcctta
     9661 atcatctctt cggcatcgac gatattttga aggacttgtt tatactcacg gtaggctatt
     9721 acggtgtcac gattggaagc ttcttctttt gaaagctcca taaaacgctt ggtgtctgaa
     9781 acgacatcag ggtcactcag caattctcct agttcttcat aacggtcttc tacagcttgt
     9841 agttgatcat agatgttcat ttttcttcat tctccttatt gatttcaggg gcaaaataat
     9901 gtttacggca aaccgagata taggtttcat taccaccaat ttggatctgt tctccatcgt
     9961 aaacgggcag tccatcctgt gttcgcaaca ccatggtcgc ctttttcttg caatactgac
    10021 agatggtctt gatttcgtca atcttgtctg ctaaaagcaa gagatatttg gaaccttcga
    10081 acaattcatt gcgaaagtca tttttcaagc caaaagccat gacgggtatg tctaactcgt
    10141 ccacaacacg agctaggtca taaacatggt ggcgtttgag aaactgggct tcatcgacca
    10201 acacacagta aggtttttct ggtaggcctc ggatatagcc aaagatatcc gttgtttcct
    10261 caatcgcaag ggcagggcgt ttcatgccaa ttcgactcga cacatagcca acaccgtcac
    10321 gcgtatccag agctgaagtc ataatcacaa caccttttcc ttgctcctca tagttatagg
    10381 ccaccttgag aatctcgatt gtctttccag agttcatggt cccataacga taatacaact
    10441 gtgccatgtt tcttgcttca cgtccatttc taaatttttg ctacattcta gtatatcata
    10501 attttcttaa gctttaaacg gcaaaatgtg gtaaaatgga agaaatcaaa actagtggag
    10561 gaagctatta tgccatttgt acgcatcgat ttatttgaag gacgcacgct cgagcaaaag
    10621 aaagctcttg ctaaggaagt aacggaagca gttgtccgca acactggagc ccctcaatct
    10681 gctgtccatg tcatcatcaa cgacatgcca gaaggaactt acttcccaca aggggaaatg
    10741 cgtactaaat aagctagctt aagcagaatt gcttaggctt tttcaatctc caagtagcat
    10801 tcattgaaga aatatcctaa atttgttaca atttgaaaag aaacttggag aatttccaag
    10861 aaaatagcta ttaattaaag gaaacattat gattacacgt gaatttgata ccatcgctgc
    10921 tatctctact ccactaggtg aaggggctat tggtattgtc cgcctgagcg gaacagacag
    10981 ttttgctatt gcgcaaaaga tttttaaagg aaaagacttg aacaaggttg ccagccacac
    11041 tctcaactac ggtcacatta ttgatcctct gactggtaaa gtcatggacg aggttatggt
    11101 tggggctatg aagtctccaa agaccttcac tcgtgaggat attatcgaga ttaacaccca
    11161 cggtggtatt gcggtgacca atgagattct ccagctagct atccgtgaag gagttcggtt
    11221 ggcagaacct ggtgaattta ccaaacgtgc ctttttaaac ggtcgcgtag atttgacaca
    11281 ggccgaggcg gtgatggata tcatccgcgc caagacagac aaggccatga acatcgcggt
    11341 caaacaactt gatggttccc tttctgatct tatcaataat atccgtcaag aaatccttaa
    11401 cacacttgcc caagttgagg tcaatatcga ctatcctgag tatgacgatg ttgaggaagc
    11461 cactactgct gttgtccgag agaagacaat ggagtttgag caattactaa ccaagctcct
    11521 taggacagca cgtcgtggta aaatccttcg tgaaggaatt tcaacggcta tcattggacg
    11581 tcccaacgtt gggaaatcaa gccttctcaa caacctcttg cgtgaggaca aggctatcgt
    11641 aaccgatatc gctgggacaa cacgagatgt catcgaagag tacgtcaaca tcaatggtgt
    11701 acctctcaaa ttgattgata cagctggtat tcgtgaaacg gatgatatag tggaacaaat
    11761 cggtgttgag cgttcgaaaa aagccctcaa ggaagccaac ttggttctac tagtgctaaa
    11821 tgccagtgaa ccactgactg cgcaagacag acaacttctt gaaattagcc aagataccaa
    11881 tcgcattatt ctacttaata aaaccgacct gccagaaacg attgaaactt cgaaactacc
    11941 tgaagacgtt atccgtattt cagtccttaa aaaccaaaac atcgacaaga ttgaagagcg
    12001 aatcaacaac ctcttctttg aaaatgctgg cttgattgag caagatgcta cttacttgtc
    12061 aaacgcccgt cacatttccc tgattgaaaa agcagttgaa agcctacaag ccgttaatca
    12121 aggtcttgag ctggggatgc cagttgattt gcttcaagtt gacttgactc gtacttggga
    12181 aatcctcgga gaaatcactg gggatgctgc tccagatgaa ctcatcaccc aactctttag
    12241 ccaattctgt ttaggaaaat aagaaaaatc catgatcctt cattcggtca tggattttat
    12301 tgtctttatt agtaatctgg tcttaagacc cctgttacag ttgccttagt tgcttcgtag
    12361 tcgccatcta cgacaacctt gataatgcgt ttggcatctt cttctggtgc tggaacaaga
    12421 ggtagacgag tgggtccagc ttcaaatccc atatagttaa gaattgcctt aactggagca
    12481 ggacttggat aagagaagag agcattaacc ttaggaatga atttacgctg aattgctgcg
    12541 gctttcttca tatcgctttc tgcaatggca gtaaacatct cgtgcatttc atccccattt
    12601 gtatgagagg caacagaaat aaccccatcc gccccaaggt tcatggcatg gaaagcatct
    12661 ccatcctcac ctgtataaat caagaactct tcaggcttgt gctcaatcaa gtaagccata
    12721 ttagccaagc tagtacattc tttgacaccg ataatatttg gatggtcagc caagcgaagc
    12781 atggtttctg gagtcaattc gacaactaca cgccctggaa tgttatagat aataattggt
    12841 aggtcagaag catctgcaat agtcttaaag tgctgataca tcccttcttg agaaggtttg
    12901 ttgtagtaag gaacaatagc aagcccagct gcgaaaccac caaattccgc tacttctttg
    12961 acaaactcaa tagagtcacg cgtatcatta gtacctacac ccgcaatcaa aggaacgcgt
    13021 ccattgacaa ccttttgtac agccgcaaac aactccaact cctcatcgtg ggtcaaagtt
    13081 ggactctcag cagtcgttcc tgcgagaaga attccatccg tatgatgggc caataaatgc
    13141 tcaatcaagg ctggaatagc atcaaagtta atagaaccat cctcatgaaa gggggtaata
    13201 aaggctgtaa tgattttaca ttcttttaaa tcttgataag acatgaaaac acctctctat
    13261 ttcaaagaag gagttcatct gaactcctaa acgatatgac tattttaatt caaatttcaa
    13321 ttcagctgtt ggacgaacca atccacgttc atgaagagtt tcagcaatct gaactgagtt
    13381 ccaagcagca cctttgagaa ggttatctga aacaacccac atgtgaattc ctttttctgc
    13441 atccaagtct ttacggatac gaccaacaaa ggtatcacgt gaaccaactg cattgatggc
    13501 ttgaggatag atttgatgaa ctacatcatc ttcaagaaca gcacctggga aggatgcgat
    13561 agctgctttg acttcttcaa ttggagccac ttcctttgtt tcaatataaa ctgactcaga
    13621 gtgagctgac aagactggaa tacgcacaca tgtcgcagat actgcaatgc tatcatcttc
    13681 cataattttc ttagtttcct tggtcatctt catctcttcg tacgtgtaat cattatcagt
    13741 gaagacatca atttgtggaa gagcgttaaa ggcgatagga taatgtttct tgtcaccacc
    13801 tgaaggcaag atttccgcat gcaaatcacg tggtttcact ccatcattca agacctcacg
    13861 aagttcacgt tgtgtctcaa gaattgctcc cataccagca cctgaaacgg cttgataagt
    13921 tgaaacaatg atacggtcca agccccattt ttggcgaacc ggctcaagag ccaccatcat
    13981 ttggattgtt gaacagttag ggcaggcaat gattccgttg tgggcatcaa gtgcatgagc
    14041 attgacctct ggaacaacca aaggaacatc tggattttga cggaaataag atgtattatc
    14101 cactactacc acgccagctt ttactgcgta tggtgcatac ttagctgatg tcgaaccacc
    14161 tgctgaaaag agagcaatat caactccttc aaaagctgtt tcagtcgttt cttcaatcgt
    14221 aatatcttga tctttaaatt tcaatgactt acctgctgaa cgtgcagaag caaggtaacg
    14281 gattttatcg attggaagtg ttgattcttc caacattttt atcatctgag caccgacagc
    14341 acctgtcgcg ccgactacag caactgtata tcccataaat aacctctttc ggaattttct
    14401 aaaaatttct ataatagaag tattatacta ctttttccat gaattgcaaa gctttttcat
    14461 cattttttgg aaaataaaaa tccccaatcg ctagactagg gattaagagg catcttcatg
    14521 tgatgagcag gttcacacaa ctcatcaagg tccgctcctg cgttatgacc tccttatgct
    14581 caatagtagc ccgaagacta cctatcgact catcgcatct actattctac taaagagaat
    14641 gacttttgtc aacagacgaa actctttata ctcttcgaaa atctcttcaa acttcgtcag
    14701 cgtcgccttg ccgtatgtat ggttactgac ttcgtcagtt tcatctacaa cctcaaaaac
    14761 atgttttgag ctgacttcgt cagttctatc tacaacctca aagcagtgct ttgagcaacc
    14821 tgcggctagt ttcctagttt actctttgat tttcattgag tattagtttt atttacacag
    14881 gtgcataaga aatatattgg aaaaattggg ggcagacata acaaaaaccc ttgtaaatca
    14941 agggcttttc tgtctattta atgctaattg ccgggattga accggcgacc tcatccttac
    15001 catggatgcg ctctgccaac tgagctaaat cagcttactt aagaagtata ctataatcag
    15061 aaaaacttgt caagtttcct tagaaatttt ccataggaaa aagccaggta agaactacct
    15121 agcttatctt cttctattta cttaaatcaa ttcgacgacc aattttacca ataataatcg
    15181 ctccaataat caatgaagca aattcaccga ttcctgttgt aaaccaagta aggaagaatg
    15241 gtaattctgc tacaatattt aactctgcag cgatagtaaa catggaaatt gaaaagagga
    15301 ttgaaaagaa gaaatgatct tttcgaatca atccgttaaa gaggtaatcc ttgctatatt
    15361 ttgcaaaaag ccaaacacct aaactgagga atactaaggt tgaaccacca ccaacaaaga
    15421 catctagcag tccgaagcta aagaaattag caatcataca accgatggta actccgatga
    15481 tgtacttagg attgtaaaaa gccataaagt tcatcatttc tgaaatacga aactgataag
    15541 caccatagct gatggcattt aggggcggtg tgacagtcaa aacgacgtag atagcagcga
    15601 cgattgcaat atctgcaaca tcacgaatag ttaatttttt catcttttct cctttggcgg
    15661 ttatccgcgt gtaatatgct tggtgaaaga agctaagcac caagggttga ttcattcaac
    15721 cttactagta tagcacaata gcctgcttta tgctatactt aaagcatgaa aaaacctatt
    15781 caaaaatttt ttaacaatga aatcttagcc tatctctttt ttggccttgc gacaactcta
    15841 gtttcgattc tctcacgact agtcatttat caactaagtc atcaggaact ccttgctacg
    15901 gccttggcaa atattatcgg tatcttattt gcctttatca caaatgatac gattgtattt
    15961 aagcaaaaaa agaaaaatag acttatccgt ttagtgaaat tttctcttgc tcgcctttct
    16021 acttttctgt tagacttgtt tttaactttt ctctttgtaa cccaatttcc tcacatcata
    16081 gggcaattcg taactgaaaa cattgataag ataaatgcta ttgaaacaat aattgcacaa
    16141 atattcatta ttatcatcaa ttatatcttg agcaaggtct atatttttag aaaataatgc
    16201 tccatttatg agccttttaa ttgccttcta taatgattta ggatacaatg aaacacgaaa
    16261 cattgaaagg aaaacagtat gttaaaaaat ctaaaatcgt tcttgcttcg aggaaatgtt
    16321 attgaccttg ctgtcggtgt tgtcattgcc tctgcttttg gtgctatcgt tacttcactt
    16381 gtaaacgaca ttatcactcc tcttatttta aatcccgctt tgaaagctgc taaagtcgaa
    16441 cgtatcgctc aactttcttg gcatggagtc ggctatggta actttttaag tgctattatc
    16501 aattttatct ttgtgggtac cgccctcttc tttattatca agggcattga aaaagcacag
    16561 aaactgactg gcataaagaa agaaaaaact gccgaaaaaa aaccaaccga attggaagtc
    16621 cttcaagaaa taaaagctct ccttgagaaa aaataatcga ttaaaaggat ctagcgcaaa
    16681 gctaaatcct tttttcttta ctctttgggt aacttgcctg ctttttcaag catttttttg
    16741 ataagataag gcatcttaac attctcccgc ccttttttct caatcagttt tttcacaaat
    16801 tctggcattt gtaaatcttc agattcagct aaaatgtttt tcgtctcgtc tgaaggaact
    16861 aactcatcaa aggtctcttc ttctgggaga aattccttaa actcttgatt ttcattgact
    16921 ctaactgtat tgaccaaagc atcaatcaaa cgagaatctg tatttggcat tggtggacga
    16981 tggtagttta ccccaaattc ctggcataag tcataacatt ctacatcatt gtcaaacaag
    17041 acctcaatat gctcactgat gaaactaata ggtacaaaaa tataatggtc tggatgttct
    17101 gtctgttctc tgagatactc caaaacatct ggtttaatcc atggaatacc aatatcactc
    17161 tcactctgcc aagtgttggt atattgttct gaactcaagc ccagtttttc agcgactagc
    17221 ttgctatttt cgaaaatttg gtctatataa ggatcaccaa agtccaaggc aaaaatgggc
    17281 acactatggg ctgaaaagat gactttaaag ctgtcttgct ttacttcttc ttttaaaatc
    17341 ttagcaattt catctgccca atagtttaag agcgcttctt cctgatacca gtccttaatg
    17401 accaaaaatt gaatttgttt gctttctaaa aatttttcat agcccatgac agagtagaaa
    17461 gaataatggg gctccaaaat caagcaaata cactgttcaa ttccgtctgc ttccatttga
    17521 ccgatcacat ctggaataaa gggactggaa aacttattag caaagtagac actgtattca
    17581 ttccccagcc tagcttctac caaggcaacc tcttgacggg taattttttg cagaggcgtg
    17641 cctccaattc gtacataatt atcatagaga gtctgaatct cgtggtcttg gggtctcacc
    17701 ccacgacgaa tatttgtgaa aaaatcagct acaccttcaa aggtaatctc ttctggtgaa
    17761 ccgaaggtca tcattaaaat tgcttttttc atgactgctt ccttgtgatg tttttatcaa
    17821 gtttatttta tcattaattc atcgataagt aaacgctaac atagggacat tctcaatctt
    17881 ctgtcttttg tttttctctt ctttctatga tacaatggaa aaaataaatt caaaaggagt
    17941 ttttttatga cttatccaaa tctcttggac cgcttcttaa cctacgttaa ggtcaacacg
    18001 cgctctgatg aacactctac tactactcca agtacacaga gtcaggttga cttcgcaaca
    18061 aatgtcctaa ttcctgaaat gaaacgtgtt ggactgcaaa atgtttacta tctaccgaat
    18121 ggttttgcta ttggaacctt gccagccaac gatccgtctt taacacgtaa gattggtttt
    18181 atatcgcaca tggatactgc tgattttaat gctgaaggag tcaatccaca ggtaattgaa
    18241 aactacgatg gtggtgtgat tgaactaggg aattctggtt tcaaactcga tccagctgac
    18301 ttcaagagtc ttgaaaaata tccaggacaa acgctcatca caacagatgg aacaaccttg
    18361 ctaggtgctg atgacaagtc aggaattgct gaaattatga cagccattga atatctaact
    18421 gctcatcctg aaattaagca ctgtgagatt cgtgttggtt ttggtccaga tgaagaaatc
    18481 ggtgttggtg ccaataaatt tgatgcagaa gattttgatg tggattttgc ctacactgtt
    18541 gatggtggtc cactaggtga acttcagtac gagactttct cagccgctgg tgctgaattg
    18601 catttccaag gccgtaatgt ccaccctggt actgccaaag ggcagatggt caatgccctt
    18661 cagctagcaa ttgattttca taatcaactt ccagaaaatg accgacctga gttaactgaa
    18721 ggttaccaag gtttttacca tctaatggat gtgacaggta gtgttgagga ggcgcgtgca
    18781 agctacatca ttcgtgattt tgaaaaagat gcctttgaag cgcgtaaagc atccatgcaa
    18841 tctatcgctg ataagatgaa tgaagaactt gggagcaacc gtgtcactct caacttgaca
    18901 gaccagtact acaatatgaa agaagtcatt gaaaaagata tgactccaat taccattgct
    18961 aaagccgtta tggaagatct aggtatcacg cctattatcg aaccaatccg aggtggaaca
    19021 gacggctcta agatttcctt tatgggaatc ccaactccga atatctttgc aggtggcgaa
    19081 aatatgcacg gacgttttga atacgttagc cttcagacta tggaacgtgc agttgatacc
    19141 atcattggca tcgtacgtag cttataaaag ctaaaaagac gaggtagctc agctacttcg
    19201 cctttctttt tattctactg gtttttcttg atttccagta gttgtagaag attctgttgt
    19261 tttattttct gaagttgatt cagcaggttt agaatctctt gtattgcttg gtttgttttc
    19321 gtcgctagca gtttcaatgt tagattctgc agttgcgttt ggttggttct cagcactggt
    19381 gttatcacca tttgcttcag catttcttgc tggacttgtt tcttcacttg cgctagcttt
    19441 tgactggatt tgatgattca aaactagaat agcttttgtc gattcaagta aagctgtttt
    19501 gtctttactc ttagcagaaa gttgatctaa taatgcatcc accttatcaa agtccgcatc
    19561 agatccatta ttactttcta aataagagtg aagcgacatg agaatatcgt agagtttttg
    19621 atagagtaca agtgtctgag gatcttgctc agcattttcc ttttcttgtt gaagggcgct
    19681 agcgatacga gtcaaaacat cttttacctg actgtttact tcatccaagt ctgcatcagc
    19741 cttgtttgtg gcagctttta gattttctac ttcttctgcc aaggattgtc tgattccttc
    19801 ttcatggatt tgttccaaga gttgatttgc cttgttcaaa agactttcta cttcttcctt
    19861 gctatctgtc gcagattatt ggttgctatc taccatgtac tcctaaaaca ggagagttat
    19921 aatccaagat tacaaggcct tacagaaata agaaatccag ataagacaat gttcgtccaa
    19981 gacgctattc gcttcgcaca gcagcacgga ttcaatatgc tttaatttta aagtttaggt
    20041 gtcaagacct ctttttagtg tgcccaaaat ttagagaagt aatcaatcaa ctaactttta
    20101 tttttttcaa actttcagta aactgaccta aagctaactc aatctgtctt tgtagatgct
    20161 tctgctatca gctagaagtt gatctacttt tgccaagact gccttctcat caaaagttcc
    20221 aggttgatag ttggattgca gggatggaat cttgtttttc aaagccgctt catatccctt
    20281 agtttgaacc ttgatgtagt gattgtggtc gccatgagga atcacaaaac cttctgaatc
    20341 ttcacttata attcgattgg catcaaaaac catgaccatc ttcttcctca tggtggacat
    20401 gtagtgacgg attacttaat acagaactag aagaacttcc tacctcttcc gtgttagagt
    20461 gtgatgggag attgttaaga gatgacttag gaatatagtg atagtgttcc ccatgtctta
    20521 ctatataagc atcacctata tctctgacaa tatcattagg gttaaaaaca tatgtggctg
    20581 ctaattcacc tgccgacaag tcactctcag gaatgaaatg atagtgacca ccatgtggta
    20641 ctatagtagg ttgaaataga atatgagcaa attgataagg ggattttaaa gtaatttcta
    20701 acaatgattt agaaactatg atgtgctatt ctaaattcaa ctcactatat ataaccatca
    20761 tcggtagtat aacgtccctg taattttgct acagatactt ctgcactagc tcctttatcg
    20821 tctttaccat gttcttgttt ttggcgattg atttcatctt ttgttcgtac attttctgca
    20881 tgagcttaat ctttaaggta aacataatac tttccatcta ccttaatact atatcctccc
    20941 ttaacctaac tgacgatatc ttgatctttc ggctgatagt tgggggcttt cattaatggc
    21001 tcttcactaa agagcgcatc aaaaggaact ttaccattat agcagtgata atgatcgcca
    21061 tgagaagtta cataaccttg acctgtaatc ttaataacaa tttgttttgc ttgaattcct
    21121 tctttttgac taacctagtc tggagtcaaa ttttcagtct tcttagtgtc tttattactg
    21181 tttacatatg aaacacgatt tttatctgta ttggcctgtt agctatgttg gttcagagca
    21241 taaacacaca gacttaagga aaggataaca acagatccag ctgctatata tttcttttta
    21301 aatttcataa ttac
//
LOCUS       DBERMR010000038        19032 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.038, whole genome shotgun sequence.
ACCESSION   DBERMR010000038 DBERMR010000000
VERSION     DBERMR010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 19032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19032
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.038"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..374)
                     /locus_tag="ACL8A1_001502"
     CDS             complement(<1..374)
                     /locus_tag="ACL8A1_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HGS1202252.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRYQAGQDKKESNRVAYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGG"
     gene            complement(532..2982)
                     /gene="phtA"
                     /locus_tag="ACL8A1_001503"
     CDS             complement(532..2982)
                     /gene="phtA"
                     /locus_tag="ACL8A1_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HGS1202253.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQTSQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGDHYHF
                     IPYSQMSELEERIARIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPGPQPAPNLKIDSN
                     SSLVSQLVRKVGEGYVFEEKGISRYVFAKDLPSETVKNLESKLSKQESVSHTLTAKKE
                     NVAPRDQEFYDKAYNLLTEAHKALFENKGRNSDFQALDKLLERLNDESTNKEKLVDDL
                     LAFLAPITHPERLGKPNSQIEYTEDEVRIAQLADKYTTSDGYIFDEHDIISDEGDAYV
                     TPHMGHSHWIGKDSLSDKEKVAAQAYTKEKGILPPSPDADVKANPTGDSAAAIYNRVK
                     GEKRIPLVRLPYMVEHTVEVKNGNLIIPHKDHYHNIKFAWFDDHTYKAPNGYTLEDLF
                     ATIKYYVEHPDERPHSNDGWGNASEHVLGKKDHSEDPNKNFKADEEPVEETPAEPEVP
                     QVETEKVEAQLKEAEVLLAKVTDSSLKANATETLAGLRNNLTLQIMDNNSIMAEAEKL
                     LALLKGSNPSSVSKEKIN"
     gene            complement(3236..4969)
                     /gene="ptsP"
                     /locus_tag="ACL8A1_001504"
     CDS             complement(3236..4969)
                     /gene="ptsP"
                     /locus_tag="ACL8A1_001504"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGS1202254.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            complement(4975..5238)
                     /locus_tag="ACL8A1_001505"
     CDS             complement(4975..5238)
                     /locus_tag="ACL8A1_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HGS1202255.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            5589..5807
                     /gene="nrdH"
                     /locus_tag="ACL8A1_001506"
     CDS             5589..5807
                     /gene="nrdH"
                     /locus_tag="ACL8A1_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HGS1202256.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            5888..8047
                     /gene="nrdE"
                     /locus_tag="ACL8A1_001507"
     CDS             5888..8047
                     /gene="nrdE"
                     /locus_tag="ACL8A1_001507"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HGS1202257.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            8235..9197
                     /gene="nrdF"
                     /locus_tag="ACL8A1_001508"
     CDS             8235..9197
                     /gene="nrdF"
                     /locus_tag="ACL8A1_001508"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HGS1202258.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            9379..10140
                     /locus_tag="ACL8A1_001509"
     CDS             9379..10140
                     /locus_tag="ACL8A1_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGS1202259.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNISNLDTIVSDSKLSDSILFKLSKHIKVIKP"
     gene            complement(10419..10526)
                     /locus_tag="ACL8A1_001510"
     CDS             complement(10419..10526)
                     /locus_tag="ACL8A1_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HGS1202260.1"
                     /translation="MTSEELKSLGKWYVSTGKEWDTISFDSDFMPFQQS"
     gene            complement(10649..12055)
                     /gene="lacG"
                     /locus_tag="ACL8A1_001511"
     CDS             complement(10649..12055)
                     /gene="lacG"
                     /locus_tag="ACL8A1_001511"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HGS1202261.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHACAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGIGRR
                     VAPDYVSRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            complement(12270..13961)
                     /locus_tag="ACL8A1_001512"
     CDS             complement(12270..13961)
                     /locus_tag="ACL8A1_001512"
                     /EC_number="2.7.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0019197 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase complex [Evidence IEA];
                     GO_function: GO:0015155 - lactose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose-specific PTS transporter subunit EIIC"
                     /protein_id="HGS1202262.1"
                     /translation="MDKLIIFIEKGKPFFEKLSRNIYLRAIKDGFIAGMPVILFSSIF
                     ILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQ
                     INYMSTLLAAIVGLLMLAADPIENGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
                     TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARHFVGASVAESIGKFFAPLFSA
                     ADGYLGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLV
                     LNPIFFIPFIFAPIANVWVFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFI
                     LAALLIVVDVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAG
                     VDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLPE
                     FDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQEQFD"
     gene            complement(13963..14280)
                     /locus_tag="ACL8A1_001513"
     CDS             complement(13963..14280)
                     /locus_tag="ACL8A1_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGS1202263.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            complement(14316..15152)
                     /locus_tag="ACL8A1_001514"
     CDS             complement(14316..15152)
                     /locus_tag="ACL8A1_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HGS1202264.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEKVIVIGKGIAFGKKKGDLIAENH
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            complement(15386..16366)
                     /gene="lacD"
                     /locus_tag="ACL8A1_001515"
     CDS             complement(15386..16366)
                     /gene="lacD"
                     /locus_tag="ACL8A1_001515"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HGS1202265.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQYQTEEP
                     TVAQMEELKILVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAVFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            complement(16368..17297)
                     /locus_tag="ACL8A1_001516"
     CDS             complement(16368..17297)
                     /locus_tag="ACL8A1_001516"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HGS1202266.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDDQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKLVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLEELKEVLQESLFTGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAGLLIKA
                     NVLGMLNAQEKMTGHVNMANYQALYDQLIVKEV"
     gene            complement(17308..17823)
                     /gene="lacB"
                     /locus_tag="ACL8A1_001517"
     CDS             complement(17308..17823)
                     /gene="lacB"
                     /locus_tag="ACL8A1_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HGS1202267.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHLESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            complement(17854..18279)
                     /gene="lacA"
                     /locus_tag="ACL8A1_001518"
     CDS             complement(17854..18279)
                     /gene="lacA"
                     /locus_tag="ACL8A1_001518"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HGS1202268.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            complement(18436..18933)
                     /locus_tag="ACL8A1_001519"
                     /pseudo
     CDS             complement(18436..18933)
                     /locus_tag="ACL8A1_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234238.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
     gene            complement(18949..>19032)
                     /locus_tag="ACL8A1_001520"
     CDS             complement(18949..>19032)
                     /locus_tag="ACL8A1_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HGS1202269.1"
                     /translation="TTYRNHRKRFGLRMNLSAGIINHELGF"
BASE COUNT         5622 a         3949 c         3442 g         6019 t
ORIGIN      
        1 ccacccttga tttcattgac aatgtctgaa tccttcaact gataattcgg atctttcatg
       61 aggagctctt cactgatgat ggcatcataa ggaaccttgc cattatagta atgataatgg
      121 tctccatgag aggtcacata accttgatcc gtaatcttga taacaatttg ttcggcgttg
      181 atcccctccc tcttactgac ttcatctggt gtcaagtttt ctgccttttg accagcctga
      241 tcaccatcta tataagcaac tcgattagac tctttcttat cctgaccagc ttggtaacgt
      301 ccaagctcat aggaacaaac acttagggca aggactgcca ctgaacctgc tagatatttt
      361 ttattgattt tcattctttc ctcttttctt aagtatattt gattcactat aaagtaataa
      421 accggttaat taactagtta atagtttact ctttttaata ttgcttgtca agaattttac
      481 atatatacat aataaaaatg aaagcctctt tatcgagact ttcatttttc attagtttat
      541 tttttcctta cttacagatg aaggattact tccttttaac aacgcaagta atttttctgc
      601 ttctgccatg atactattgt tatccataat ttgaagagtc aaattatttc gtaaaccagc
      661 tagagtttct gttgcattgg ctttcagact agaatccgtt actttcgcaa gcaaaacttc
      721 tgcttctttg agttgggctt ctactttttc agtctctact tgagggactt ctggctcagc
      781 aggtgtttcc tctactggct cttcatccgc tttgaagttc ttatttggat cttcactgtg
      841 gtctttcttg cctaacacat gctcactggc attgccccat ccatcattag aatgtggacg
      901 ttcgtcaggg tgttctacgt agtacttaat cgtcgcaaac aaatcttcca aggtatagcc
      961 atttggagct ttgtatgtgt gatcatcaaa ccaagcaaat ttaatgttat ggtaatgatc
     1021 cttatgagga ataatcaaat taccgttttt aacctcaact gtatgctcaa ccatatatgg
     1081 aagtcgaacg agtggaattc gtttttcccc tttcacacga ttgtaaatag ctgctgcact
     1141 atctccagtt ggatttgctt taacatctgc gtctggagat ggaggtagga tacctttttc
     1201 tttagtatag gcttgagctg caactttttc cttatcagaa aggctgtctt ttccaatcca
     1261 gtgactatgg cccatatgag gcgttacata tgcatctcct tcatcactga ttatatcatg
     1321 ttcatcaaaa atataaccat ctgacgttgt atacttatca gctaattgag caatacgaac
     1381 ttcgtcttca gtatactcaa tttgagaatt tggcttgcca agtcgctctg gatgggtaat
     1441 tggtgctagg aatgccaata aatcatctac caatttttct ttattagtcg attcatcatt
     1501 caagcgttct aataatttgt ctaaggcttg gaaatcagaa ttacgaccct tattttcaaa
     1561 caaggcttta tgagcctcag ttaacagatt atatgcttta tcataaaatt cttggtcacg
     1621 aggagcaaca ttttcttttt tagcagttaa agtgtgtgaa acactctctt gttttgataa
     1681 cttgctttca agatttttaa cagtttcaga tggtaaatct ttcgcaaaga cataacgaga
     1741 gatgcccttt tcttcgaata catatccttc cccaactttt cgtaccagct gactaaccaa
     1801 agaagaattt gagtctattt taagatttgg tgcaggttgc gggcctggac taggttccgg
     1861 agtcggttgt ggacttggtt gttctggcct tgaatctggt acccaatggt ttgaacgata
     1921 acgaagggga ataatacgag cgattcgttc ttccaattca gacatttgag agtaagggat
     1981 gaagtggtaa tgatctccgt gtggcactgc aacacctcta gctgttcgac ttgtgatttg
     2041 cgctgggtcg aaaacaaggc catcagattc tacatgtcgt tgactcaaag gcagtttgta
     2101 gagctgtttc aagagactat caatgtcatt actttgactt gtttgactgt tagtgttgct
     2161 gttgttgctt gtgttagtat ttgtagttcc tggattgctt acagaaggta cccagtttgt
     2221 tcttgaagtg ttatcgctat tttgtcggcg ataggttctt gaatttgaca gatttccccg
     2281 accagatagg aaggcttctg cagcagccaa ctcgctagct gataactcat tcttaggaat
     2341 gtaatggtaa tgatctccat gaggaacgat ataagcatca ccagtatcct ctatgatatc
     2401 agaagcatta aagatataac catcatctgt agtatagcgt ccttgcgaac gtgccaaggc
     2461 aacagcacca tcgtttcttg gagtcccacc ttcacgatgt tgactatgtt cttgtttttg
     2521 tcgattgatt tcctcttttg tacggacgtt atccgcgtgg gcagcatcct taaggtaaac
     2581 atagtatttt ccatctacct tgataacata tccacccttg acctcattaa caatatcctc
     2641 atcttttagc ttatagttcg gatctttcat gagtaattct tcactgaaaa tcgcatcata
     2701 aggaacctta ccattgtaat aatgatagtg gtcgccatgt gaagtgacat agccttggtc
     2761 tgttatcttg atgacgattt gctcagcatt gattccttca cgcttgctaa cctcatcagg
     2821 agtcaaattc tccgtttttt gcgtcgcttg ttttccatct atataggaaa cacgattatt
     2881 ttccttaacc gttctagctt gatacagtcc caactcgtaa gaacaaacac ttaaaatcaa
     2941 agctgccgca gaaccaacaa ggtatttctt attaattttc atcgaaactc tctttctatt
     3001 tgatatttct agttgcaatt catttctaaa ttgcagaaac tcctctctat tacttaactg
     3061 gttaatagtt tactcctaaa tctccaactt gtcaagaatt ttatgaacca tttaagtatt
     3121 ttttgttctg actactactt tttcaaggtt ggactttcta taagaaagat tttcttgaaa
     3181 attcttttta aaatatcaaa aaaatccctg taactgagtt gcagggactt ttcgattaat
     3241 caaaattaac gtattctttt tgaagttcaa gaacttcttc cattgttgag cattctgtaa
     3301 gggcacggtt tgcgtactct tccatcttag ctgtgtcgag tttcttcatc aagctgcgtg
     3361 tacgaagtac agatgttgct gacatagaga actcatccaa gcccattccg acaagaagtg
     3421 gaacagcttg ttggtcacca gccatctcac cacacatacc agcccattta ccttcagcgt
     3481 gagctgcttt gatcacattg ttaatcaagc gtaggattga tgggttgtat ggttggtaaa
     3541 ggtatgaaac ttgttcgttc atacggtctg ctgccattgt atattggatc aagtcgtttg
     3601 taccaattga gaagaagtca acttctttag caaattggtc tgcaagcata gccgctgcag
     3661 gaatctcgat catgatacca acttggatgt tatccgcaac tgcaacacct tcagcaagaa
     3721 ggtttgcttt ttcttcatca aagactgctt tcgctgcacg gaattctttc aagagcgcaa
     3781 ccattgggaa catgatacgc aattgaccgt gaacagacgc acgaagaaga gcacggattt
     3841 gtgtgcggaa catagcatct ccagtctcag agatagagat acgaagagca cggaatccaa
     3901 ggaatgggtt catttcgtga ggcatatcga agtaaggaag ttccttatct ccaccgatat
     3961 ccattgtacg aacgacaaca ggtttaccgt tcattccttc aagaacagcc ttgtatgctt
     4021 catactgctc atcttcagtt gggaagtctt gagaatccat gtacaagaac tctgtacggt
     4081 aaagtccaac agcttctgca ccgttgttgt taacaccttc aacgtctttt ggagtaccga
     4141 tattagcagc caactcgaag tgtttaccgt cagcagtcac tgtttgagca tctttcaaga
     4201 gcgcccattc agctttttgt ttcgcataag cttcaccagc tgctttaaat tctgccgctt
     4261 gttcatcagt tgggttgata ataacctcac cagtgatccc gttaacagca aggatgtcac
     4321 cgtctttaac gatttcagtg atgttgtttg tacctaatac agcagcaatt tcaagtgtac
     4381 gtgccatgat agctgagtgg cttgtacgtc caccgatgtt ggttacaaaa gcttttacaa
     4441 agtttttgtc caattgagct gtatctgaag gagtcaagtc atgcgcaatc acaatcactt
     4501 cttcattgat agaagctggg tttggcaatt ttttaccaag aaggtttgcc aatacacgtt
     4561 ttgtcacgtc gcggatatcc gctgcgcgtt cttgcatgta tgggttgtct tccatgcctt
     4621 caaagatagt gataaacata tctgtaactt ctttcagacc tgcttctgca ttcactttct
     4681 tcgcacggat agtttccttg atttggctga tcatttctgg gtcagcaaga accattaagt
     4741 gagcatcaaa aacttgagct gcttcttcac cgagcgtacc tactgctttc tcgcgaataa
     4801 cagaaagctc gtcttgtgat gcctgtagag cggcatcaag gcgagcttct tctgcgtttg
     4861 tatcttcgac tgtaatagtc tcaaatgaca aatccggctg aacgagtaga tatgcttttg
     4921 caactgcaac accgtcagat gctgcgattc ctttaagcat ttctgtcatt tcccttatgc
     4981 caatccttct ttttccattg tttctgagat tgcagcgata gcgtcatctg catctgcacc
     5041 ttcagctgag atagttacgt cagcaccttg accaacacca agactcataa cacccataat
     5101 tgatttaagg ttaactgatt tacctttgta ctcaagagtg atatctgaag caaatttgct
     5161 agcagtttgt accaacaatg ttgctggacg tgcgtgaata cctgtttctg ccactacgtg
     5221 gaaatcttta gaagccatag tttgactctc cttttgttct ttcttttttt gagttatatg
     5281 tgataaccct tacaatttag tattatacca tcttctagga tttttttcaa gcattttctg
     5341 gattttcctt caatttcctt tcagataact tgctgaaaat cttctatcct agataacaaa
     5401 acacaggata tagtttcttc aaaaacaatt tataggataa tcattgctat ttcacaatac
     5461 aaacactata tattgtgttt atatatgaaa acaacaaaag aacaccacta tatttagttt
     5521 aaatcattga caaaatttta ttttctgata tactaagata gtattaaatt ttgaagagga
     5581 gttacacaat ggtaaccgtt tattctaaaa acaattgtgt ccaatgtaaa atgaccaagc
     5641 gtttcttgga cagtaataat gtctcttatc gtgaaatcaa tcttgatgag caacctgagt
     5701 acgtcgatca agttaaagag ctcggtttta gcgcagctcc tgttatccaa acaccaactg
     5761 aagtcttttc aggtttccaa ccaggaaaac tgaaacaatt agcataatct tagtacatca
     5821 tccagaagaa actgctttta gggctaactt agaagccttt cttttgtaat tagataaggg
     5881 aaattttatg ggattaaaac atcttgaaga cgtgacttac ttccgtctta ataacgaaat
     5941 taaccgtcct gttaatggac aaatcatgct tcataaagat aaggaagcct tggatgcttt
     6001 ctttaaagaa aatgtagttc caaacactat ggtttttgat tcaatcaaag acaaaatcaa
     6061 ttacctcatt gaacacaact acatcgaaac agcctttata aagaaatacc gtccagagtt
     6121 cttggaagaa ttggctcaat ttatcaaaga ccaaaacttc caattcaagt cattcatggc
     6181 agcttataaa ttttacaatc aatatgcctt aaagactaat gacggtgaat actatcttga
     6241 aaatatggaa gaccgtgtct tcttcaacgc cctttatttc gctgatggga atgaagctgt
     6301 tgcaatcgat attgccaatg aaatcatcca ccaacgctac caacctgcta ctccttcctt
     6361 cttgaatgct ggacgtgctc gtcgtgggga gttggtatct tgtttcctaa tccaagtgac
     6421 ggatgatatg aactctatcg gacgttctat caactcagct cttcaacttt cacgtattgg
     6481 tggtggtgtg ggaattaccc tcagcaacct tcgtgaagct ggtgcaccta tcaaaggcta
     6541 tgaaggagct gcttcaggtg tcgttcctgt catgaagctt tttgaagaca gcttctctta
     6601 ctccaaccaa ttggggcaac gtcaaggtgc cggtgttgtc tacctcaacg tctttcaccc
     6661 agatatcatc gctttccttt caactaagaa agaaaacgct gatgaaaaag tacgtgtcaa
     6721 gactctatca cttggtgttg tagtacctga taaattctac gaattggctc gtaaaaatga
     6781 agaaatgtac ctcttcagcc cttactctgt agaaaaagaa tacggtgtgc cattcaatta
     6841 catcgacatt actgaaaaat acgatgaatt ggtcgcaaat ccaaatattc gcaagacaaa
     6901 aatcaaggcg cgggatttgg aaactgaaat ctctaaattg caacaagagt ctggctatcc
     6961 ttatgtagtc aacatcgata cggctaaccg tgcaaatcct gttgatggaa agattatcat
     7021 gagtaacttg tgttctgaga ttcttcaagt tcaagaacca agcttgatca acgatgctca
     7081 agaattcctt caaatgggaa cagatgtttc atgtaacctg ggttcaacca acgtggttaa
     7141 catgatgact tcacctgatt ttggtcgttc tatccgtgct atggttcgtg cccttacttt
     7201 cgttacagat agttcacata tcgtagctgt accaacgatt gaccatggaa atagccaagc
     7261 ccacaccttt ggtctcggtg ccatgggact gcacagctac cttgcccaac aactcattga
     7321 atatggttca cctgagtctg ttgaatttac aagcatctac tttatgctta tgaactactg
     7381 gaccttggta gagtcaaaca atattgcgcg tgaacgtggt attaccttcc acaactttga
     7441 aaaatcagac tatgctaacg gaagttactt tgacaagtat gtaactggtg aatttgttcc
     7501 aacatcagac cgtgttaaag aactcttcaa aaatgtcttt atccctggtg ttgctgattg
     7561 ggctgaactt cgcgacaagg ttcaagaaga tggtctttac caccaaaatc gccttgctgt
     7621 agcgccaaat ggttctatca gctatatcaa cgacgtttct gcttctatcc acccaattac
     7681 gcaacgtatc gaagaacgcc aagagaagaa aattggtaaa atctactacc ctgctgctgg
     7741 cttgtctaca gaaaccattc cttactacac ttctgcctac gatatggata tgcgtaaagt
     7801 catcgatgtt tacgctgctg cgactgaaca cgtggatcaa ggactttcac tcaccctctt
     7861 catgcgtagt gacattccaa aaggccttta cgaatggaag agggaaaata aacaaacgac
     7921 acgtgacttg tccatccttc gtaactatgc ctttaacaag ggaatcaagt ctatctacta
     7981 cgtccgtacc tttacagatg acggtggaga agtcggcgcc aaccaatgtg aaagctgtgt
     8041 gatttagttt ctatcataga aacactattt ttccctaagc agcactgata aaatcaaatc
     8101 tactatgtca taacataatt tatgtaacat tttaaaagtc gggcttccga ctttttgtgc
     8161 gatactccta tagaattatg gtaaagtaga aataattgtg agttcgcaat actaaacaca
     8221 gaaagagatt ttaaatggaa acttactaca aagccattaa ctggaatgcc atcgaagatg
     8281 tcatcgacaa atcaacttgg gaaaaactga cggagcaatt ttggctcgat acacgtattc
     8341 ccttatcaaa cgacttggat gactggagaa agctatctaa caaagagaaa gacttggtcg
     8401 gaaaagtttt tggtggttta acccttcttg acactatgca atctgaaact ggggttcaag
     8461 cccttcgcgc agacatccgt acaccacatg aggaagctgt tttcaataac atccaattta
     8521 tggaatctgt ccacgctaaa tcttactcat caatcttttc taccttgaat actaaggctg
     8581 agattgaaga aattttcgaa tggaccaata ccaatcctta cctacaaaag aaggctgaga
     8641 ttgtcaacga aatctaccta aacggcagcc cacttgaaaa gaaagttgcc agcgtcttcc
     8701 tcgaaacctt cctcttctac tctggtttct tcactcccct ctactatctc ggtaacaaca
     8761 aactagccaa cgttgcagaa atcattaaat tgattattcg tgacgagtct gttcacggaa
     8821 cctacattgg ttacaaattc caacttggtt tcaatgaatt gcctgaagaa gagcaagaaa
     8881 aactcaaaga atggatgtac gacctactct atactcttta tgaaaatgaa gaaggctaca
     8941 cagagagtct ttatgacggt gttggttgga ctgaagaggt taaaaccttc cttcgctaca
     9001 atgccaataa agctctcatg aacatgggac aagatccact tttcccagat tcagctgaag
     9061 atgtcaaccc aatcgttatg aacggtattt caacaggaac ttccaaccat gacttcttct
     9121 ctcaagtcgg aaatggttac cttcttggcg aagttgaagc catgcaagac gatgactata
     9181 actacggttt agactaattc tccatcactc tcaatcatca aaaagtaaac tagaaacatc
     9241 aaagttataa cccaacaaaa atatcgaaag ttttgaaaac aaacttcgat atttttgttt
     9301 ttgtttcatt ttgttgtttt tgtatttgaa tgatgagttt gtttatggta agatagtagt
     9361 aggaaacgag gtgataatat gctcaaacaa gaaaaattaa ccaaaatttt agagatagta
     9421 aatagtaaag gaaccataac agttaaacag attatggacg aaatagccgt ttcagatatg
     9481 actgcaaggc gctatttaca ggaattagct gataaagatt tgctgattcg tgtgcatggt
     9541 ggagctgaaa aacttcgaac caactccctt ttgactaatg agcgatcaaa tattgaaaaa
     9601 caagccctcc aaacggcaga aaaacaagaa atagcccatt ttgcaggcag tctagtagaa
     9661 gaaagagaaa ctattttcat tggaccagga acaacattag agttttttgc gcgtgagttg
     9721 cctattgaca atatccgcgt cgtaaccaac agtctacctg tttttctgat tttaagcgaa
     9781 cgaaaattaa cagatttgat tttgataggt ggaaattatc gcgatattac aggtgctttt
     9841 gttggcacat tgaccctaca aaatctctct aatctccaat tttctaaagc tttcgttagc
     9901 tgtaatggta ttcaaaacgg agctctagct acttttagcg aggaagaggg agaggctcaa
     9961 cgcatcgctt taaataattc taataaaaaa tatttactcg cagatcatag taagttcaat
    10021 aagtttgatt tttatacttt ttataatata tcaaatcttg atactattgt ttcagattct
    10081 aaactaagtg attcaatcct ttttaagcta tctaaacaca ttaaagtcat caagccttaa
    10141 taaactgaaa gagcctgggt aacaactcaa tttcagaaga aaaaaagtaa atcttcccac
    10201 aataaaacgc atagtatcag gtgtttcaac acctgatact atgcgttttt ctatatcaaa
    10261 gactttttgc ccaattcctc aaagctatgc attgagaaat ctgcgactcg cttcctagtt
    10321 taaagttgct ctttgatttt cattgagtat aagcaacgtg attagataag agttttacag
    10381 agtactaaat ataagattta acttttttga gaaattggtt acgattgttg aaacggcata
    10441 aaatctgaat caaaggagat agtatcccat tctttaccag tcgagacata ccattttcca
    10501 agacttttca attcttcact cgtcaaacac ggtatttgag agcatttttc gaaatttata
    10561 atataaactt tttttcataa atagattgga taaaatcttt gaacatcttt ttgactcact
    10621 aaaattctat atctaatgac taattctact agtctataat ctgagtttcc gctactttct
    10681 tgtaccagtg agctgatttc ttaggataac gttcttgagt ttcaaaatct acgtagaaga
    10741 gaccataacg tttctcataa ccgtttgacc atgagaagac atccattaat gaccaaatga
    10801 agtaaccttt tacattagct ccatctgcaa tcgcatcaga caagatctcc aagtgttgtt
    10861 tcacgtaatc aatacgacca tcatcgtaaa cagtgttatc aacgaactca tctttatagc
    10921 cgagaccatt ttcagtgatg taaattttct tgtagttagg ataatctttc ttcacacgca
    10981 tgatttggtc atacaaacct tgagggtaga taatccaatc ccaatccgtg cgtgatacat
    11041 agtcaggagc tacacgacga ccaatacctt tgatttgata cttagagctt cctttttcac
    11101 ctttaccatt atggataatt tcagtttctc catcaaaggc ttccatccag tcactcatat
    11161 agtagttgat tcctaggaaa tcattcaagt cttttgcagc ttctaatgct gtaaaatctt
    11221 cttcacgaag atccaaacta ccaccattga ctgataagat atggttgaca ccttccatgg
    11281 tttcagctga atagcgacct agataagttg cgtctaagat gaatttattg tggatgatat
    11341 cttccaactc tgctgcacga acatctgctg gattttcagg atctagagga tatttagtag
    11401 gcagggcgtg aacaacacca atttcccctt tatagccttt ctctttgtac aattttaccg
    11461 cgcatgcatg agacaccatc atattgtggt gtgattgaaa gactttggca aggtcgtact
    11521 ggatacctgg agggaatttc ccaaccaaat attgaccatc accgattggt ccaatttcat
    11581 tgaaggttgt ccaatagttt acttctggaa attcttcaaa acagaaggca gcgtagtcta
    11641 caaaatgttc gatattttca cggtttaaga agtctccatt tgagtggaga gcttctggcg
    11701 tgtcaaagtg atgaagagtt acaaagggct caacatgacg tttgtgacac tctgcaaata
    11761 aattatgata aaactcaaca cctttagcat ttacttgacc ataaccagtc gggaaaatac
    11821 gtgaccaggc aatagaaatt cgaatgccat tgacaccata ctcttctgct agcttgagat
    11881 caactggata tcgattgtaa aaatcactgg ctggttctgc agtgtaccag tagttatctt
    11941 caagatattt atcccacgct actggtcctt taccatcagt atgtgtagca ccttctgctt
    12001 gataggcagc tgttgcgcca ccaaaaataa agtcttttgg aagtgttttt gtcatttttt
    12061 tcacctattt cttgatttaa ataaagtaaa agtgagagga gaggagtcag tgagccatcc
    12121 ttctccatct cacttcttgt accaagtcac cgaattgtga catgaggagg taaaaacgat
    12181 atctttttac cgacgaatta ataaggcgat taggaaattc gccatagcga tttccgtaac
    12241 gataggagac tgtttcacag ttcctagcct taatcgaatt gttcttgtac gaatgctagg
    12301 gcaccttttc catcacgagt taatttgatg tattgagcgc cttctgtttt cgctagttta
    12361 ataccgagct tatctgtttc tgccttcata tcttcaaagt ttgaagcaac ttgaggagca
    12421 aggataacca aatcaaactc tggtaacatt tcacggtgag caccatagcc gcctgctgct
    12481 gctttcacag ggacattgta ttctgctgct gccttattca aagcatttgc aaggagtcca
    12541 cttgtacctc caccagcaca gagaacgagg acatttgttt cttcagtgat ggtattttgt
    12601 gctgcatcga cacctgcttt ttcaagaata gcatccgctt ttgcagtgtt gaagtttgca
    12661 gcaacttttt ctttcaattc atcattagac ttacctgaac gttcttcttc aagaatttgt
    12721 tcatcataga ccttaaggaa tggatagtaa atgacaacgt caaccacgat tagaagagca
    12781 gcaagaatga atgatagaac ttggaagtta gttccaagaa ctagacctag tggagctggg
    12841 gttgtccatg gtagattagc agtaaatgaa ttcattccaa gtgtttcaat aaagaatttg
    12901 aaaacccata cgtttgcgat aggagcaaaa ataaatggaa tgaagaagat tggatttaat
    12961 acaagtggtg caccaaacaa gattggttca tttacaccga agaaggtagg aactactgaa
    13021 gcacgtccga ttgcacggtt acgttttgat tttgttaacc acataaacat gaatggaaca
    13081 accagtgtcg caccagtacc acccatggta acgataaaca tttgtgtacc agaagtaaga
    13141 atcttgtcag cgtgcatacc ttgttggaga aggttcaagt tgacttcagc atttgcatag
    13201 gtaatcgctg cgatagcagg ttcaacgata gatggaccat gaatcccaac aaaccagaag
    13261 aaggcaaagg caccaaagat aatggtaata ccaaggtagc catcagctgc agagaagagt
    13321 ggtgcaaaga atttaccgat tgattcagct acgcttgcac caacaaaatg acgagctagt
    13381 aaatcaagag cataaagtga aacaactgag agagtgaatg gaatcacatc tttaaagact
    13441 tgtgagatat ttggtggaac ttcgtcaggc atacgaatag tgacgttgtt cttaacacaa
    13501 accttataga tggctacagt aacaaaggca gcaaggaagg ctgaaagcaa accttttgtc
    13561 cccaagaatc cagtagctag accattttcg ataggatcag ctgccaacat caacaaacca
    13621 acaatcgctg ctaacagcgt tgacatatag ttgatttgat tggttttctc catgctccgg
    13681 tttactgagt cggtcaatga cttagctgtt gtaccagcta ccaagagagc cagaataccc
    13741 atagaatagc tataaggttt catcagaaag gctacaactt catcagacca tttaaagccc
    13801 catgaatttg gtacaaaggc aatcaagata aagatacttg agaagagaat aacaggcata
    13861 cctgcaatga aaccatcttt tatcgctctt aagtatatat ttctcgataa tttctcaaag
    13921 aaaggctttc ctttctcaat aaaaattatt aacttatcca ttttatttaa ctcctctctt
    13981 gtaaagttca attaaatggt gcattaggtc ttttaacaag atagttgtca tcaagtgatc
    14041 ttgaccatgc atcatggtca cactatacgc taagtcctca ccagaagctt ccttagtcaa
    14101 tagacttgtt tgcgcgtggt gagcctctgc aatacagcta ccagcttcct ctaccagagc
    14161 gtccgctttt tcaaaatcac cagcttcagc agccttcaag gcttccaata gttttgaacg
    14221 agcatcgcca gcataggcta cgatttcaaa acctaacaat gttacttctt ctctattcat
    14281 gatagaattc tccttatata gttttaaata aatttttatg acaataaacg ttggatatgt
    14341 agaactagat aaactcgttc acttttatac aaatcaagac ccgtatgttg cgtaatcaca
    14401 tcatagatct tggaaccaat ctcgaaggct tttggatagg attgtttaat atgatcttcc
    14461 atatccagaa gtgattggtt atcatctcta gatctgtcta aataatccaa gaaataattc
    14521 aaatggatca taaagcgatc atagaaatgg ttattctttt tagttcgttg aattgcatag
    14581 tccgttaaaa cttcttcaac attcctgaga atttctttcc tcttatcaat cgactccaca
    14641 agttccactt cattttcacc ttcagcatta atgaaatgat aagcaatccg aataatttcg
    14701 tcctcaggaa aatgatctgc tagcttctga cggtaaattt caaaagcttc atttgcgatt
    14761 tgaaaagcga caggatactt agcagaaata tctggcagat tactatcctt gtaccttcct
    14821 tgagttagag cttgataaga acagtaaata tgatctgtca aggttacata gagatactct
    14881 tgaatcggat aatgatattt ctttgatagc ttatcaatga tttcataggt cactgtgata
    14941 aaatcaagcg gaacatcttt gagaagagcc ataaagtttt ctctggactc ttcggtcttc
    15001 atccgaaaga ttttctcaac atgattttca gcaatcaaat cccccttctt ctttccgaat
    15061 gcaatcccct taccaattac aatcaccttt tctcccttat catttctgac aagcgagaca
    15121 ttgtgattca ttggatttag aattcgatac atgtcaacct ccttttatat cttaaaggta
    15181 tataagtaca aaaaatgacc acaaatgaac tagaaaatca gctgatttct aattcacctg
    15241 tgatcatgcc taatattact tagtaacact caccttgtat taacttgtga tttaagtata
    15301 gcacaagtaa gttattttgt aaacctttac atacaacttt ttttaaattt ttttagattc
    15361 atgttcctaa actaggagga ctttcttaca cacgttcttt ccatgaagtc gctgttgttt
    15421 gaagaacttt attgagctca tcaatgtttt caaatccagt tgtgcgaagc cattcgcgag
    15481 ctgctgcttc accatctttg atgtaagctt caactgaccc tgcccatgta gcacgaccac
    15541 aaagaactcc gttaaagttt gcacctgatt catgagcaaa tacaagagta tcttggaaga
    15601 gtttagctga tacaccagca ctcaagtaga tgtatggcaa gttcgttgct tcatcttgtg
    15661 ctttgaagaa gactgctgct tcttcacgtg tgtaaaccac ttcaccctca gcgaagcctt
    15721 caacatattt aatgttaaca ggaacttcaa ctttcaagac atcaatgtta aaacgtgggt
    15781 ctgaaaagac tttcatagca ccgataactt tgtgtggttt tactttagcg tattctacag
    15841 aacctgcgtc cgcaattttt tcatcgtaag caaggatttc aaggaagaat gggatatctt
    15901 cagccacaca ctcagaaccg atgcgttcga tgtaggcttg tttttcttga ttgagttcgt
    15961 ctgagctatc tacatcatag taaagcaaga atttaactgc atctgcacct tcttctttaa
    16021 tacgttttgc agaccaaaca tccaagcagt ctggcaagcg ttttgtgctt gttgtgtcat
    16081 aacctgtttt ttcataagca aggagaagac cagctttttc atcaagagcc ttagttgctg
    16141 gaagtccata ctcagggtca agaagcatag atgaagcgta tttagtcaat tcatctgcta
    16201 ccaagatttt aagttcttcc atttgggcca cagttggttc ttctgtttgg tattgagcca
    16261 tgaggcgttt caaagcacca cgttggtcaa atgcaagagc tgagatgata ccattttcat
    16321 cagaaagttt ttctaagcgt acacgttttt gttctgttaa agccatttta tacctctttt
    16381 actattaatt gatcatatag agcttgatag ttggccatgt tgacatgacc agtcattttt
    16441 tcttgagcat tgagcatacc aaggacattt gccttgatga gtaatcctgc atccgattct
    16501 ttgtgaagaa gtcctgaaga aattcctgcc acagtagagt ctccagatcc aacaggattt
    16561 accacctgaa ttctaggaat atctaccttg tagaaagtgt caccatgttt ggcaaaagta
    16621 ccgttggcac caagtgaaac gataatccat tcaatccctg taaacaaaga ttcttgaagg
    16681 acttctttta attcctccaa atcctcagaa acttctcttc ctagaagctg agacaattcc
    16741 tcattattgg gtttgattac tgtcggttta tggggtgatt caagaaccgc ctgaagtgct
    16801 gcaccagagc agtccaagac tactagcttg ccagcttgat tagcaagctc taccaagctc
    16861 gcatagtaat caactggaag gccagctggc agactacctg agatggctac tacttcaact
    16921 gactccaaga gttttttgaa atgttccaaa aagtcttgac cttcctgttc caatacttca
    16981 ggaccttttt caagaacttc tgtttggttg tcaccgtgga gaatagcgat acagttacga
    17041 gtttctccct gaatagaaaa gaaatctttc tttacttgat catcgatatg ttcaaccaaa
    17101 aactcaccaa gtttgccacc cactaaacca gtagcaagaa cagaatcacc aaattctgaa
    17161 agtactcggg taacattaag tcccttacca ccagccgttt tggttacatc caccacacga
    17221 ttgacagtat caatcttcaa ctcatccaag ggataggaaa tatcaatgga tgggttcatt
    17281 gtgactgtta aaatcatagg tcacctctta gtcgtggtat tctccgcgat cccatttttc
    17341 aaggaattct gtaaagaagt ttgcgtctgt ttgttgagca ttgtgacttt caaggtgttc
    17401 aattttcgca atcaatttct tgttttcttc agatggtttg tattcagcat gaatgaaagc
    17461 ttcgatgata tcacacataa gcaattcacc agtaatctta ccaccaaagc cgataacgtt
    17521 agcgttcaat tgttctttag cataaagggc tgttgtcata tcacgaacca aggcagaacg
    17581 aacacctgga actttattta cagcgttgtt gataccaaca ccagtaccac agatacatac
    17641 cccaagatca gcttggccgc tagttacagc ttcccctact tttttaccaa agattgggta
    17701 gtgagtccgt gtatggtcat atgtaccaaa gtcaatgact tcatatcctt ttgatttcaa
    17761 aaattctgaa accgccattt tttcatctgt tacgatgtgg tcgcatccaa ttgcaattct
    17821 catttttaac ttacctttct tactgtaatc taattagccc attttgttca acatgtcaac
    17881 ccggatttga tgacgtccac cgtcgtattt accattaaca aatcctttag cgatgttttt
    17941 agccaattca tcaccaacaa gttgtgcccc catagtgatc atacgtgagt tgttgtggcc
    18001 acgagtcata taagctgaac gttcgtcaga tacttctgca gcaaccattc ctttgatctt
    18061 agttgcaacc ataaatggac cagctccata agcatcaatc acgataccaa ggttttgttc
    18121 ttctttgttt acttctgcag caacagcgag agtcacatca acaaagtctt gaccttcagc
    18181 tgtaacatcc acaaggtgga agttttcttt ttctaagaag tctttcacaa cttctttcaa
    18241 tctcaaacct gcagcatctg caccgataac aatagacata ttgtatactc ctttttattt
    18301 tttctatgct ggattccagc agtgtattgg ctgtctttaa aaaacagtca attagtcctc
    18361 acaaacataa tatactacta ttgtttgaag aagtcaatag tttgtgtttg tttttgttat
    18421 ttatttttta cttttttata aaacttctat ctcaaaccct aattcttcaa cctgattggc
    18481 ttctaagaga caaacattct tcttatcttc taaatgatct ccttcttcaa ggaatgttga
    18541 caagccagac catggttcaa aggtaatgaa aggtcccttg ttcaaagttg accagatgat
    18601 gaggtttgga aattctgcaa aatgcacttt caatccctta tcatgtttaa gagaacgaag
    18661 ggcaattgtt cgagattgca attcatccaa agtcactgca tctgtgctga aaagatcata
    18721 actaagatct atttcttttt gtccctctag ccatggactt ctatcttgga aatccaacat
    18781 acctgtttct gggaaaggac gtggaacaga gcaagtctct tctttctcag actctagata
    18841 ataatcttca tagactccat catcaagtag aggacaatta aatcctggat gtccaccaat
    18901 aaagtaaggc atgaccttgc tagtttcttt attagacttc ctgcaaaact agaatcctag
    18961 ttcatgattg ataataccag cactcaaatt cattcgtaat ccgaagcgtt tacgatgatt
    19021 tcgataggtt gt
//
LOCUS       DBERMR010000039        18833 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.039, whole genome shotgun sequence.
ACCESSION   DBERMR010000039 DBERMR010000000
VERSION     DBERMR010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 18833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18833
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.039"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(1..473)
                     /locus_tag="ACL8A1_001521"
                     /pseudo
     CDS             complement(1..473)
                     /locus_tag="ACL8A1_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            complement(576..713)
                     /locus_tag="ACL8A1_001522"
                     /pseudo
     CDS             complement(576..713)
                     /locus_tag="ACL8A1_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            755..1075
                     /locus_tag="ACL8A1_001523"
                     /pseudo
     CDS             755..1075
                     /locus_tag="ACL8A1_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884744.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
     gene            1196..2140
                     /gene="manA"
                     /locus_tag="ACL8A1_001524"
     CDS             1196..2140
                     /gene="manA"
                     /locus_tag="ACL8A1_001524"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HGS1202270.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLHDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDDLLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTVKADDLRSTLLVSNDFFAVYKWEITGKVDFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGSHFILPSDVEAWTLEGQGLELIVSHP"
     gene            complement(2161..3639)
                     /locus_tag="ACL8A1_001525"
     CDS             complement(2161..3639)
                     /locus_tag="ACL8A1_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HGS1202271.1"
                     /translation="MIFLTDLRKHDRIVKSINQTEGYLTTQVAFSYFEKGDQSLTMSE
                     KSQWGSKLGFILASAGSAIGLGSVWKFPYMTAANGGGGFLLIFLISTILIGFPLLLAE
                     FALGRSAGVSAIKTFGKLGKNNKYNFIGWIGAFALFILLSFYSVIGGWILVYLGIEFG
                     KLFQLGGTGDYAQLFTSIISNPAIALVAQAAFILLNIFIVSRGVQKGIERASKVMMPL
                     LFIIFVVIIGRSLSLPNAMEGVLYFLKPDFSKLTSAGLLYALGQSFFALSLGVTVMLT
                     YASYLDKKTNLVQSGISIVAMNISISIMAGLAIFPAMSAFNIQSEGGPSLLFIVLPQL
                     FDKMPFGTIFYVLFLFATVTSSVVMLEINVDNITNQDNSKRAKWSVILGILTFVFGIP
                     SALSYGVMADVHIFGKTFFDAMDFLVSNLLMPFGTLYLSLFTGYIFKKALAMEELHLD
                     ERAWKQGLFQVWLFLLRFFVSSFQSSSLWSSLPNLCNQKGLE"
     gene            3744..4493
                     /locus_tag="ACL8A1_001526"
     CDS             3744..4493
                     /locus_tag="ACL8A1_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HGS1202272.1"
                     /translation="MYHIKEAAQLSGVSVKTLHHYDKIGLLVPLKSENGYRTYSQEDL
                     ERLQVILYYKYLGFSLEKIAELLKEERTDLLPHLTRQLDYLTRERQHLDTLISTLQKT
                     IQEQKGERKMTIEEKFAGFSYQDNQKYHQEAVEKYGQEVMGQALERQKGHEDEATAAF
                     NQVFQTLAQNLQAGLPATATENQEQAAKLLQAIRTYGFACSIEVFGHIGKGYVYNPEF
                     KENIDKFGSGIAQYTSDAIAAYVQTNAEKIG"
     gene            complement(4513..4968)
                     /locus_tag="ACL8A1_001527"
     CDS             complement(4513..4968)
                     /locus_tag="ACL8A1_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HGS1202273.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWPGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEELSEFFYYKNGDDWLYDLK"
     gene            complement(4995..6170)
                     /locus_tag="ACL8A1_001528"
     CDS             complement(4995..6170)
                     /locus_tag="ACL8A1_001528"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGS1202274.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITELEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVLDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(6229..7074)
                     /locus_tag="ACL8A1_001529"
     CDS             complement(6229..7074)
                     /locus_tag="ACL8A1_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HGS1202275.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            7199..7756
                     /locus_tag="ACL8A1_001530"
     CDS             7199..7756
                     /locus_tag="ACL8A1_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGS1202276.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            7775..8242
                     /locus_tag="ACL8A1_001531"
     CDS             7775..8242
                     /locus_tag="ACL8A1_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HGS1202277.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMNDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            8331..8960
                     /gene="upp"
                     /locus_tag="ACL8A1_001532"
     CDS             8331..8960
                     /gene="upp"
                     /locus_tag="ACL8A1_001532"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HGS1202278.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            9134..9724
                     /gene="clpP"
                     /locus_tag="ACL8A1_001533"
     CDS             9134..9724
                     /gene="clpP"
                     /locus_tag="ACL8A1_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HGS1202279.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            9803..10051
                     /locus_tag="ACL8A1_001534"
     CDS             9803..10051
                     /locus_tag="ACL8A1_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HGS1202280.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            10152..11312
                     /locus_tag="ACL8A1_001535"
     CDS             10152..11312
                     /locus_tag="ACL8A1_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS1202281.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            11580..12449
                     /locus_tag="ACL8A1_001536"
     CDS             11580..12449
                     /locus_tag="ACL8A1_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HGS1202282.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILGISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            12453..13409
                     /locus_tag="ACL8A1_001537"
     CDS             12453..13409
                     /locus_tag="ACL8A1_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HGS1202283.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILSVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKSPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            13409..14173
                     /locus_tag="ACL8A1_001538"
     CDS             13409..14173
                     /locus_tag="ACL8A1_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1202284.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLDYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            14173..14883
                     /locus_tag="ACL8A1_001539"
     CDS             14173..14883
                     /locus_tag="ACL8A1_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1202285.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRRSSGKIEFLGQEIQKMPAQKIVASGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            15191..15847
                     /locus_tag="ACL8A1_001540"
     CDS             15191..15847
                     /locus_tag="ACL8A1_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HGS1202286.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKISILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            15955..17050
                     /gene="prfB"
                     /locus_tag="ACL8A1_001541"
     CDS             join(15955..16026,16028..17050)
                     /gene="prfB"
                     /locus_tag="ACL8A1_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HGS1202287.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            17068..17760
                     /gene="ftsE"
                     /locus_tag="ACL8A1_001542"
     CDS             17068..17760
                     /gene="ftsE"
                     /locus_tag="ACL8A1_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HGS1202288.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            17753..18679
                     /gene="ftsX"
                     /locus_tag="ACL8A1_001543"
     CDS             17753..18679
                     /gene="ftsX"
                     /locus_tag="ACL8A1_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HGS1202289.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
BASE COUNT         5763 a         3559 c         4075 g         5436 t
ORIGIN      
        1 cctactccta ttggagacca acaagatccc agtttactga ttctaccgaa accagcctca
       61 tcttggtaca tcaagcgaac cttacgaaaa cgtctactgg ttttaaagcg ctttcttgtc
      121 ttcttgaatt gagattttat ttttagacgc aacaatggtt tgagcgtctg ctttcttagg
      181 atgttctgga cgtggcataa tatttcgcca accatggcgc ttcaacagtt gatagaaggc
      241 atcacgtgtg taggaacgac ctaacttctt tttataagcc tgaaataagg catcaattgt
      301 aacaaattct cctgcctctg tagccttcaa atggcgagca agaaaggctt tctcttcctc
      361 aactgtcata tatgcatggt tacgaccacc acgtgtttct tgaaggagag agtcgagtcc
      421 gaactcctca tattttttac atttcgccaa atcgttgttt gattacagtc tatagtgtat
      481 tgaatctata acagtacacc ttagctgcta aaatatttct ataaattagt ttgactttcc
      541 tgatagagtt gttcacatct tatttcaaac cactataaaa gctctataat ctctttataa
      601 gatttgccca tcagacgaaa tagaacgatt tgaaggcgtt tatgatattt agctgtacga
      661 gagtctttta aaagtgtttt gatggttttg atttcttctt tagttgattt catatcactg
      721 ttatataatg ctttttttat ttgagtctag tattatcgta gaaaatggac tcggtgctat
      781 ggatacaccg gatgagaatg gttatgtagc agatgactat cggattactt acttagaggc
      841 ccacatcaag gccatgcgag atgccattta ccaagacggg gttgacttgc ttggttatac
      901 gacttggagc tgtatcgatc cggtttcagc tggcacaggg gaaatgaata agcgctatgg
      961 ctttatctat gtagatcgag ataatgtagg aaatgggact ctcaaacgta gcaagaagaa
     1021 gtccttctat tggtacaagg atgtcattga tagcaatggt gcaagcatcg agtagagcac
     1081 atttgtttac tatttttata aaatcttctc cctgctttat aagatgtaaa aaagagttca
     1141 aatggaactc tttttttgct ataatgaggg gagaaaaatc agacaggaga ttaagatgtc
     1201 agaaccatta tttttacaat cagttatgca agaaaaaatc tggggtggta ccaagctaca
     1261 tgatgagttt ggctacgaca tcccaagtga aaaagtcgga gaatactggg ctatctcagc
     1321 tcatctaaat ggtgtttcta aagttgccaa tggtcgttac gagggaacag atcttgctac
     1381 tttgtatgcg gaacaccgcg aattgtttgg caatcgtcct gagcctgtat ttccgctttt
     1441 gactaagatt ctggatgcta acgactggct cagcgttcaa gttcacccag acgatgctta
     1501 tggactagag catgaaggcg aacttggaaa aacagaatgc tggtacatca tcgctgcgga
     1561 tgaaggttca gagattatct acggtcacaa tgccaagtca aaagaagaac tccgccagca
     1621 aatcgaggac aagaactggg atgacttgtt gaccaaagtc cctgttaagg ctggagattt
     1681 cttctatgta ccaagcggga ctatgcacgc tatcggggcg ggtatcttaa tccttgaaac
     1741 ccagcagtct agcgatacca cctatcgcgt ctatgacttt gaccgcaagg atgacaaagg
     1801 caacttgcgt gaacttcatc ttgaaaaatc catcgatgtc ttgaacattg gtgagccagc
     1861 aaatagccgt cctgtaactg ttaaagcaga tgatttgcgt tccactctcc ttgtatctaa
     1921 tgatttcttc gcagtttaca agtgggaaat tactggaaaa gttgactttg aaaagacagc
     1981 tgactacagc ttattgagtg tcttagctgg tcaaggtcag ctaactgttg acgggaaaaa
     2041 ttatccaatt caaaaaggca gccactttat cctaccaagt gatgttgaag cttggactct
     2101 ggaagggcaa ggtttggaat tgattgttag ccatccataa aaagaaaggg cctgagttca
     2161 ctactcaagt cctttttgat tacataaatt gggcaatgaa gaccacaatg atgatgattg
     2221 gaatgacgaa acgaagaaac gaagaaggaa gagccagact tggaacagtc cttgtttcca
     2281 tgctctttca tcgagatgga gttcctccat tgcaagagcc tttttaaaga tatagcctgt
     2341 aaaaagtgaa aggtagagag ttccaaatgg catgaggaga ttggaaacca agaagtccat
     2401 agcgtcaaag aaggtcttac caaaaatgtg aacatccgcc atgacaccgt aagatagggc
     2461 tgaaggaatg ccaaagacaa aggtcaaaat tcctaaaata acactccatt tagcacgctt
     2521 gctgttgtcc tgattggtga tattgtctac attgatttcc agcatcacga cagaagaagt
     2581 gacagtcgca aagaggaaga ggacgtagaa aatggttcca aaaggcatct tgtcaaagag
     2641 ttgaggcaag acgataaaga gcaggctggg tcccccttca gactggatat tgaaggctga
     2701 catggctggg aaaatggcta gacctgccat gatggatatc gagatattca tggctacgat
     2761 ggagattcct gactggacta gattggtttt cttgtccaag taagaagcat aggtcaacat
     2821 gactgtaacc cctagtgaga gggcaaagaa agattgtccc agagcataga ggagaccagc
     2881 gctagtcagt tttgaaaagt ctggtttgag gaagtaaaga accccttcca tggcatttgg
     2941 caaactgaga gagcgaccga tgataacaac aaagatgata aagagcaggg gcatcatgac
     3001 tttcgaagct ctttcaatcc ctttttgaac cccacgtgat acaataaaga tatttaataa
     3061 gataaaggct gcttgagcta ctagggcaat ggctggattt gaaatgattg aagtaaataa
     3121 ctgagcataa tcacccgttc caccaagttg gaacaatttc ccaaactcaa tacctagata
     3181 gactagaatc catcctccga taacactgta aaaagataag aggataaaga gggcaaaggc
     3241 gccaatccaa ccgataaagt tgtacttgtt attcttgccc agttttccaa aggttttgat
     3301 agcggaaacg ccagcactac ggccaagggc aaactcagcc agcaggagag ggaaaccgat
     3361 taaaatagtg gaaatgagaa agattagtaa aaagcctcca ccgccattag cagcagtcat
     3421 gtagggaaac ttccaaacgg aaccaagccc gatggccgag ccagcagatg ctagaataaa
     3481 accaagtttc gacccccatt gtgatttttc tgacatagtt aaactctgat ctcctttttc
     3541 aaaataagaa aaagctactt gagttgtcaa atagccttct gtttgattga tagacttgac
     3601 tatcctatca tgttttctaa ggtctgtcaa gaaaatcatt ttcaagtttt cacacctttc
     3661 tcaaaaaagt taaaaaattt tctcaaaaac gcttgactct gacctaaggg gaggggttat
     3721 actatcattg taaggaggaa atcatgtacc atataaaaga agctgcgcag ctttcaggtg
     3781 tctctgtcaa gaccctgcat cactatgaca agataggact cttggtccca ttaaagtcgg
     3841 aaaacggcta tcgaacctat agtcaagagg atttggaacg ccttcaggtc attctttact
     3901 acaaatatct aggcttttct ttagagaaaa tagcagagct gttaaaggaa gaaaggacag
     3961 atttattgcc ccatttgact aggcagttgg actatctaac tcgcgaaagg caacatctgg
     4021 ataccttgat ttccaccttg caaaaaacta ttcaagaaca aaaaggagaa agaaaaatga
     4081 ccattgagga aaaattcgcg ggatttagct atcaagacaa tcaaaaatac caccaagaag
     4141 cggtagagaa atatggtcaa gaagtcatgg gacaagcgct tgaacgccaa aaaggtcacg
     4201 aagacgaggc tacggccgcc tttaaccaag tctttcaaac tttggcacaa aatcttcaag
     4261 ctggtttacc tgcaacagca accgaaaacc aagagcaagc agccaagctc ttgcaagcca
     4321 ttcgcactta tggatttgcc tgctctattg aggtattcgg tcatatcggt aaaggttacg
     4381 tctacaaccc agagtttaag gaaaacattg acaagtttgg ttctggaata gcccagtaca
     4441 cgtcagatgc cattgcagct tacgttcaga caaatgcaga aaaaataggc taggaatttc
     4501 ctagcctatt ttttacttca aatcataaag ccagtcgtca ccgtttttgt agtaaaagaa
     4561 ttcactgagt tcttcttcta gaaacacacg aagcatatca gacatatcat cggttgcaag
     4621 ttttagatga gaaagatttt caaagtcctc ccaccaaact ttcccttcgt ctgaagactg
     4681 gagttcacca gtaaagtgtt ctgtcttgta aaaaaggaca acataacgat aatccttgtc
     4741 gtcataccag tttttgatac cacagagttg gggtttggaa atgatcagac cagtttcttc
     4801 tttcacttca cgaatgacag catcgacaaa ggattcgcca cgttcaacat gaccaccagg
     4861 aaaagtaatg ccaggccagt cgggactaac tcggtcttgg accaggacct tatctccgtt
     4921 tttaatcata cacatgttaa caaattcgac tgcctctctt ctgttcattc ttcacaacct
     4981 ttaatcttta atctttaatc ataatgcaga cttcccgcca cccatccggt acagagggca
     5041 gaagtaatgt taaagccgcc cgtgtgggca ttgatatcca gtacctcgcc agcaaagtgg
     5101 agcccaggaa cgagtttgct ttccagagtt ttaggattga tttccttgag actgactcca
     5161 cccttggtaa caaaggactt tgcaagggac atttttccag ttacaggaat tttaagttct
     5221 ttaatggact ggacaagttg ttctcgttcc ttttcagtca gctgtttgac tttttcagga
     5281 tatccttgta caaaaaattc ggccaagcgt tctggtaaca aggtttttaa agcgtttttc
     5341 aaggattttt cccgattttc ttctagaaat gtaaccaagt ccttctcaga aagttgaggc
     5401 aaaacatcga gtgagagaac ctccccacct ttgacaaagc tagacatgcg tagggcagca
     5461 ggacctgaca aaccaaagtg ggtaaagagt aaatcatgag tgatgacatg cttaccataa
     5521 cttagggtca catcgtccag agaaatccct tgtaaggctt tatgtggaaa atctgttaat
     5581 aaaggacttt cagcagcctc aagctcggtg atggtatgct taaaatggcg agcaatctcg
     5641 tgaccaaaac cagtcgaacc agtcgaagga taagacttac cacctgttgt gacaatgagt
     5701 ttctcacaag tgaaggtttg atccgctgac ttaaggacaa actggtcatc tactttttta
     5761 acagaaacga tttctatttg agtagcaact tgaccaccta gttcagtgat tttcttttcc
     5821 aaagcttcga taatagtccg agacttgtca ctggctggaa agacgcgtcc gtggtcttcg
     5881 accttaagtt taacaccatt ttctgtaaaa aagttgatga tgtcatgatt atcgaactgg
     5941 gagaaaacac tgtaaagaaa gcgtccgttt ccaggaattc cagctagcag gttgtctaag
     6001 ctaccattgt tggtcacatt gcaacgtccc ccaccagtcc cagctaattt ttttccaagt
     6061 ttccgatttt tttcgatgag gagggttttc tgtccataaa agctactgga aatcgtagcc
     6121 atcataccag caggtccccc accgatgaca atagtatcaa aatgtttcat agctctattg
     6181 taccacaaaa aaacaagaga tgatggtcac ctcttgtcaa gaatgcaatt aatcaatttc
     6241 atagcccatc agcaaaccgc cctcttctgc atagaaactg cagagaccag aggttggtag
     6301 aattttaata tccgcttgtg ggaaggtttc acggattcgc tctgagagct gttgacaaca
     6361 tttttcgtta ttgcgttggg ccatgacaat acggccacca gcatatccag cttttactaa
     6421 ctcatcatag gcagcttgaa ctgatttctt tgatcccctt gctttttgta gcaattcgag
     6481 agttccagtt tcactagctt ttccgaccat acgaatgttg agaaggccaa cgaccgtacc
     6541 gataagcttg ctcaaacggc cgttcttcac caagttatcg actttggcta ggacaaagag
     6601 caacttagtt ttttcttgat aggcggtgat agcttcaacc acttcttcaa aagacaagcc
     6661 ctggtcaatc aagtcattca atttttctac gagtaggtca acttcaccac cagcagataa
     6721 actatcaatc acatgaatct tagtgtcagg atggtcttcc agataaatat tctttgctag
     6781 ttgagcacta ttgtgactgc cagaaagggt acctgtgatg gttactagga aaatgttttt
     6841 ggcaccttca aatgctcgca aatagtcatc tgggcttgga caagccgatt ttgaagcttc
     6901 tgcagttgca tacatggttt ccatcatttg gtcaatatcg agactggcgt catcaacaaa
     6961 gacctgatca gctacttgaa tggttaaggg gacacttaca aaggttgtgt taatagctgg
     7021 tgttggcagt tgacgataat cacaaccaga gtcagcaata atcttccaag tcatagaaat
     7081 tctccatctt tgtcagttat acattgacaa aggttctgtc tttttttaca attatatcat
     7141 gaaaaccctt gaaacaaaag catcatccgt ctgttgtgac aagtagaaag aaaatgttat
     7201 gtctgaacgt agaatctctg aaaagtctct tgaaaatctc agaaaatcaa accaagaatc
     7261 caatttatta accagagaag ccattgaaac agccctcttg caactcttgg aaaaaaagga
     7321 gctgaccaag attagtattt ctgaattggt caaacgtgca ggtgtttcgc gtgcggcctt
     7381 ttatcgcaat tatgactcca aagaggaaat tttagagagc gtctttaaac gaactgtcca
     7441 caatattatg gaacagctgc atcattacga tttaaagaca gacctttatt tggtttgggt
     7501 tcaccttttc cgggaggcca gaaaggaagc tagagtaatt caattggcct tggattacca
     7561 tctggaaaaa atctttgtcc aagccatgca ggaatttcta gaaaaatacc atgggaaatc
     7621 aaaaggtgtc agctcttatc ttcattcctt ctggagctca gccatcgtct ctgtccttct
     7681 aaaatggatc aaggatggca tgaaggtacc agctgaaaag attgcagatt taggtttacc
     7741 attttttaaa aaatagagaa aaaggagaaa agagatgact gaaaaaagac tggcatggga
     7801 tgagtatttt gcagcccaag ccctactaat tgcgaatcgt tccacttgta aacgtgctaa
     7861 agtgggcgcg attctggtaa aagataataa ggttatttcc actggttaca atggttcggt
     7921 gtcagggact gagcattgta ttgatcacga atgtttggtc attgaaggcc actgtgttcg
     7981 cacccttcac gctgaggtca atgctatcct tcaaggtgct gagcgtggtg ttcccaaagg
     8041 atttacagcc tatgtaaccc attttccttg tctcaactgt acaaaacaat tgcttcaggt
     8101 cggctgcaag cgcgtggttt atatcaacca gtaccgaatg aacgactacg cccaatacct
     8161 ttatcaagaa aaagggacag aattgaccca tttaccactt gagacagtac agacagctct
     8221 taaagaggct gatctaatgt aaaaattatc aaaaataaat ggtttagaaa gatttttaaa
     8281 ccgttttttg gtataatgag aagaataaat tgaaagaagg aattccaaaa atgggaaaaa
     8341 ttgaagttat taatcaccca ctgattcaac acaaattgtc aatcttgcgt cgtacagata
     8401 cttctacaaa agcttttcgt gagctagtag atgagattgc aatgttgatg gggtatgaag
     8461 tacttcgtga tcttccacta gaagatgtgg aaatcgaaac accaattaca aaaacagttc
     8521 aaaaacaatt ggcaggtaag aaattggcca tcgtcccaat cttgcgtgca ggtatcggga
     8581 tggttgatgg tctcttgagc ttggttccag ctgctaaagt tggccacatc ggtatgtacc
     8641 gtgatgaaga aacacttcaa ccagttgaat acttggtgaa attgcctgag gacattgacc
     8701 aacgtcaaat ttttgtagta gacccaatgt tggcaacagg tggctcagca atcttggctg
     8761 ttgattctct taaaaaacgt ggcgcatcaa atatcaaatt tgtctgcctt gtatctgctc
     8821 cagagggtgt aaaagccctt caagaagctc atccagatgt agaaatcttt acagcagcct
     8881 tggatgaacg tttgaacgaa cacggttata tcgttccagg tcttggagat gctggagacc
     8941 gcttgttcgg tacaaaataa gatcgaaaag aagaatgact tggaaaagta tttccagtca
     9001 cgaaaggagg ttgggttttt gtttctgtct aatgaaagca gagcaaaaat ttgacctttt
     9061 ttgaccaaga tattataata gtcttatctt cagtcatttg accaacaaaa ttaaaactca
     9121 aaaggagaaa tgaatgattc ctgtagttat tgaacaaaca agccgtggag aacgttctta
     9181 cgatatttac tcacgtcttc tcaaagaccg catcattatg ctgacaggtc cggttgaaga
     9241 caatatggct aactctgtta ttgcccaatt gcttttcttg gatgcccaag atagtacaaa
     9301 agatatttac ctttatgtca atacaccagg tggttctgtt tcagctggtt tggcaatcgt
     9361 agataccatg aactttatca aggcagatgt ccaaaccatt gttatgggaa tggctgcatc
     9421 tatgggaaca gtcatcgcat caagtggagc aaaaggcaaa cgtttcatgc ttccaaatgc
     9481 tgaatacatg attcaccaac caatgggcgg tacaggtggt ggtacccaac aaactgatat
     9541 ggctatcgct gcagaacact tgctcaaaac tcgtaatacc ttggaaaaaa tcttggctga
     9601 aaattcaggt cagtcaatgg aaaaagtcca tgcagatgca gaacgtgata actggatgag
     9661 cgcccaggaa acacttgaat atggctttat tgatgaaatt atggccaaca attcattgaa
     9721 ctaatgatga tagaaggcaa actcgactgg gtttgctttt tttggtataa tagggagaga
     9781 tttcttagaa agaggattta tcatgtttga aaaagtcaat cgctctggct tgattatcta
     9841 tctttactat aatcgtgatg ccaaaaaact gcaggattat ggagatatta cctatcattc
     9901 caagaaacat cgttacttac aactctatgt tccaactcaa gaagtggagc aattggtcgg
     9961 acgcttgagc aaggaaaaat ttattaaaaa agttagggtt tgtcatatcc aagagttgga
    10021 aacacccttt gtgggcaatc tttatcgata ggaaaacgtt atcatagaaa aagttcaagg
    10081 aaaacattga caattttctg ataattcggt atattcttaa cagactattt aagaaataag
    10141 gagacaaaaa gatgaagaaa aaatttgccc tatcgtttgt ggcgcttgca agtgtagcac
    10201 ttcttgcagc ctgtggagaa gtgaagtctg gagcagtcaa cactgctggt aactcagtag
    10261 aggaaaagac aattaaaatc gggtttaact ttgaagaatc aggttcttta gctgcatacg
    10321 gaacagctga acaaaaaggt gcccaattgg ctgttgatga aatcaatgcc gcaggtggta
    10381 tcgatggaaa acaaatcgaa gtagtcgata aagataataa gtctgaaaca gctgaggctg
    10441 cttcagttac aactaacctt gtaacccaat ctaaagtatc agcagtcgta ggacctgcga
    10501 catctggtgc gactgcagct gcggtagcga acgctacaaa agcaggtgtt ccattgatct
    10561 caccaagtgc gactcaagat ggattgacta aaggtcaaga ttacctcttt attggaactt
    10621 tccaagatag cttccaagga aaaattatct caaactatgt ttctgaaaaa ttaaatgcta
    10681 agaaagttgt tctttacact gacaatgcca gtgactatgc taaagggatt gctaaatctt
    10741 tccgcgagtc atacaagggt gaaatcgttg cagatgaaac tttcgtagca ggtgacacag
    10801 acttccaagc agcccttaca aaaatgaaag ggaaagactt tgatgctatc gttgttcctg
    10861 gttactataa tgaggctggt aagattgtaa accaagcgcg tggcatggga attgacaaac
    10921 caatcgttgg tggtgatgga ttcaacggtg aggagtttgt acaacaagca actgctgaaa
    10981 aagcatcaaa catctacttt atctcaggct tctcaactac tgtagaagtt tcagctaaag
    11041 ctaaagcctt ccttgacgct taccgtgcta agtacaatga agagccttca acatttgcag
    11101 ccttggctta tgattcagtt caccttgtag caaacgcagc aaaaggtgct aaaaattcag
    11161 gtgaaatcaa ggataacctt gctaaaacaa aagattttga aggtgtaact ggtcaaacaa
    11221 gcttcgatgc agaccacaac acagtcaaaa ctgcttacat gatgaccatg aacaatggta
    11281 aagttgaagc agcagaagtt gtaaaaccat aatagaaaaa tgttgaaata gggaatgagc
    11341 ctttgactca ctccctgttt cgatatttaa tactcttcga aaatctcttc aaactgcgtc
    11401 aacgtcgcct tggattatat atgtgactga cttcgtcagt cttatctaca acctcaaagc
    11461 agtgctttga gcaacctgcg gctagtttcc tagtttgctc tttgattttc attgagtata
    11521 agaacctatc aaaaagtgag ggaaaaccct cggaattata aatagaaaga gtgaatctta
    11581 tgctccaaca actcgtaaat ggtttgattc taggtagtgt ttacgcgctg ttagccctag
    11641 gatataccat ggtttacgga attatcaagc tcatcaactt cgcccatggt gatatttata
    11701 tgatgggagc ctttatcggt tatttcttga tcaattcttt ccaaatgaat ttctttgtag
    11761 cgcttattgt agctatgcta gcgacagcta ttcttggtgt cgtgattgag tttcttgctt
    11821 accgaccttt gcgccactct actcgtattg ctgttttgat tacggctatt ggggtttctt
    11881 tcctattgga gtatggaatg gtctatctgg ttggtgccaa tacccgtgcc ttccctcaag
    11941 cgattcaaac agttcgatat gatttgggac caattagctt aacaaatgtg cagttaatga
    12001 ttttgggcat ttccttgatt ttgatgattt tgttacaagt cattgtccaa aagactaaga
    12061 tggggaaagc catgcgtgca gtatcagtag atagcgacgc ggcgcaattg atggggatca
    12121 atgtaaaccg tacgattagc tttaccttcg ctttgggttc tgctcttgcg ggtgcggctg
    12181 gtgttctgat tgctctttat tataactctc ttgagccttt gatgggggtt actccaggtc
    12241 ttaaatcttt cgttgccgca gtacttggtg gtatcggaat tattcctggt gctgctcttg
    12301 gtggctttgt gattggtcta ttggaaacct ttgcgacagc ctttgggatg tcagatttcc
    12361 gtgatgccat tgtttatgga atcttgttgt tgatcttgat tgtccgccca gctggtatcc
    12421 ttggtaagaa tgtgaaagag aaggtgtaaa cgatgaagga aaatttaaaa gttaatattc
    12481 tatggttact ccttttgtta gctggctata gcttgattag tgtactggtt tcagtcggag
    12541 tacttaatct attctatgta cagattttac aacaaattgg aattaatatt attttgtctg
    12601 ttggtctcaa cttaatcgtt ggtttttcag gacaattttc acttggtcat gctggtttca
    12661 tggcgattgg tgcctatgca gcagctatta ttggttctaa atcaccaacc tacggtgcct
    12721 tctttggagc tatgcttgta ggggctttgc tttcaggagc agttgcctta cttgtcggca
    12781 ttccaacctt gcgcttgaag ggggactatc ttgcggtagc aactctaggt gtttctgaaa
    12841 ttatccgtat ctttatcatc aatggtggaa gccttacaaa tggtgcggca ggtatcttag
    12901 ggattcctaa ctttacaact tggcaaatgg tttacttctt tgtcgtgatt acaaccattg
    12961 caaccttgaa cttcttgcgt agcccaattg gccgttcaac cctctctgtt cgtgaagatg
    13021 aaatcgctgc tgagtcagtt ggggttaata cgactaaaat taaaatcatc gcttttgtct
    13081 ttggtgccat tactgcaagt attgctgggt cacttcaggc aggatttatc gggtctgttg
    13141 taccgaaaga ttacaccttc atcaactcaa tcaacgtttt gattattgtt gtatttggtg
    13201 gactcggttc cattacaggt gcgattgttt cggctattgt tctgggaatt ttgaatatgc
    13261 ttctccaaga tgttgctagt gtgcgtatga ttatttacgc tttggccttg gtattggtaa
    13321 tgattttcag accaggtgga ctccttggaa cgtgggaact gagcctatca cgtttcttta
    13381 aaaaatctaa gaaggaggaa caaaactaat ggcattactt gaagtaaaac agttaaccaa
    13441 acattttggt ggtctaacag ctgttggaga tgtgactctt gaattgaacg aaggggaact
    13501 ggttggatta atcggtccaa acggagctgg gaaaaccacc cttttcaacc ttttgaccgg
    13561 tgtttatgaa ccaagcgagg gaacagtaac cctagatggt caccttttga atgggaaatc
    13621 accttataag attgcctctt tgggacttgg acgtactttc caaaatatcc gtctttttaa
    13681 agatttaaca gttttagaca atgttttgat tgcttttgga aaccatcaca aacagcatgt
    13741 ttttactagt ttcttacgct taccagcttt ttacaagagt gaaaaagaat taaaggctaa
    13801 agctttggaa ttgttgaaaa tctttgattt agatggtgat gcagagactc ttgctaaaaa
    13861 tctttcctac ggacaacaac gtcgtttgga aattgttcgt gcccttgcta cggaacctaa
    13921 aattctcttc ttagatgaac cagcagcagg tatgaaccca caggaaacag ccgaattgac
    13981 tgagttaatt cgtcgtatca aagatgagtt taagattaca atcatgttga ttgaacacga
    14041 tatgaatctg gtcatggaag taacagaacg tatctacgta cttgactatg gccgtttaat
    14101 cgctcaagga actccagacg aaattaagac caataaacgc gttatcgaag cttatctagg
    14161 aggtgaagcc taatgtctat gttaaaagtt gaaaatcttt ctgtgcatta cggtatgatc
    14221 caagcagttc gtgatgtaag ctttgaagtt aatgaaggag aagttgtttc ccttatcggt
    14281 gccaacggtg caggtaagac aactattctt cgcaccttgt caggtttggt tcgacgaagt
    14341 tcaggaaaga ttgaattttt aggtcaagaa atccaaaaaa tgccagctca gaaaatcgtg
    14401 gcaagtggtc tttcacaagt tccagaagga cgccacgtct ttcctggctt gactgttatg
    14461 gaaaatcttg aaatgggagc tttcttaaag aaaaatcgtg aagaaaatca agctaacttg
    14521 aagaaggttt tctcacgctt tcctcgtctt gaagaacgga agaaccaaga tgcagccact
    14581 ctttcagggg gggaacaaca aatgcttgcc atgggacgcg ccctcatgtc aacaccaaaa
    14641 cttcttcttt tagatgaacc atcaatggga cttgccccaa tctttatcca agaaattttt
    14701 gatatcattc aagatattca gaagcaagga acaacggtcc tcttgattga acaaaatgcc
    14761 aataaagcac ttgcaatctc tgaccgagga tatgtactgg aaacagggaa aatcgtccta
    14821 tcaggaacag gaaaagaact cgcttcatca gaagaagtca gaaaagcata tctaggtggc
    14881 taaaacaatc cagtggattg ttttagtcgg cagatggaga ttacgaagta atcatcaata
    14941 tagtccgggg gaccttttta gtcggtagat tgagattgca aacaaatctg catctacatt
    15001 gaaagcttaa tttctaataa ttgaaaaaat cgaatgaaaa atttcttacc ttcattcaca
    15061 gagctcgatt tcagagctct ttttgctagc ttattcatac ttttctgaat ttcgaaaaag
    15121 aaatgtaagc gtttgataga tttacaaaaa gattgtataa tagggataag aatagaaaag
    15181 gagaagtctc atggcagtta aagattttat gacccgcaag gtagtttata ttagtccaga
    15241 tacaacagta tctcatgcag cagatttgat gagagagcaa ggtttgcacc gtctgcctgt
    15301 tatcgaaaat gatcaattag ttggtttggt gactgaggga accattgcac aagcaagtcc
    15361 atctaaagca acaagtcttt ctatctatga gatgaattat cttctgaata agacaaaagt
    15421 aaaagatgtc atgattcgcg atgttgtcac tgtctcaggc tatgctagtc tagaagatgc
    15481 aacttatctg atgttgaaaa ataagattag tattctccct gtcgtagata accatcaagt
    15541 atacggagtt attactgacc gtgacgtttt ccaagctttt cttgaaattg caggttatgg
    15601 cgaagaaggg attcgtgtac gctttgttac agaagatgaa gttggtgttc ttggaaaaat
    15661 tgtttctttg attgtagaag aaaatttgaa tatctcccat acagtcaata ttccgcgtaa
    15721 ggatggtaag gtcattatcg aagtgcaaat cgatggatca attgatttac cagccttgaa
    15781 agaaaaattt gaagcaaatg gtattcaagt ggaagaaatc gctcgcactt cagcaaaagt
    15841 cttgtaagaa gggaagccca aaggcttctt ttttcatgaa aaggggatta gagaaaaaga
    15901 tggaaagaaa tgataaaata tgctataatg aaataatgta aaaaaggagt atttatggac
    15961 atttcagtaa ttcgtcagaa aattgacgca aatcgtgaaa aattagcttc tttcaggggg
    16021 tctctttgac ctcgaagggc tagaggaaga gattgccatc ttggaaaaca agatgacaga
    16081 acctgatttt tggaacgata atattgcggc ccaaaaaacg tcgcaagaat taaatgaatt
    16141 aaaaaacact tacaatacct tccataagat ggaagagttg caggatgaag tcgaaatttt
    16201 attggatttt ttggctgaag acgagtcagt gcatgatgaa ctggtagcgc agttagccga
    16261 acttgataag ataatgacca gctacgagat gactctactc ttgtcagaac cttatgacca
    16321 caacaatgcc atcttggaaa tccatccagg ttctggtggt actgaggcgc aggactgggg
    16381 tgatatgttg cttcgtatgt atactcgtta tggtaatgct aaaggcttta aagtggaagt
    16441 gttggattac caagcaggtg atgaggctgg tattaagtcg gtaactttat catttgaagg
    16501 gcctaatgcc tatggtctcc tcaagtcaga aatgggtgtt caccgcttag tgcgaatctc
    16561 accatttgac tctgccaaac gtcgccatac ctctttcaca tctgtagaag tgatgccaga
    16621 attggatgat actattgaag tggaaatccg tgaagatgat atcaagatgg ataccttccg
    16681 ttcaggtggt gccggtggac aaaacgtcaa taaggtttca acaggtgtac gtttaaccca
    16741 cattccaact ggaattgttg tccaatcaac agtggatcgt acccagtatg gaaatagaga
    16801 tcgtgccatg aagatgttgc aggctaagct ctatcaaatg gagcaagaga agaaggctgc
    16861 agaggtagat tctctcaaag gtgagaaaaa ggagatcact tggggaagcc aaatccgttc
    16921 ttatgtcttc acgccttata ctatggtaaa agatcaccga actagctttg aggttgctca
    16981 ggtagataag gttatggatg gggacctaga tggttttatc gatgcttatc tcaagtggcg
    17041 aattagctaa gatagaaagg aactcacatg tcaattattg aaatgagaga tgtcgttaaa
    17101 aaatacgaca acggaacgac tgctctacgc ggtgtttcgg tcagcgttca accgggggaa
    17161 tttgcttaca tcgtaggacc ttcaggagca gggaagtcaa cttttattcg ttctctgtat
    17221 cgtgaagtaa aaatcgataa aggaagccta tcagttgctg gttttaatct ggttaagatc
    17281 aaaaagaaag atgtcccgct tctacgtcgt agtgttgggg ttgtcttcca agattataaa
    17341 ttgttaccaa agaaaactgt ctatgaaaat attgcttacg ctatggaagt aatcggggaa
    17401 aatcgccgta atatcaaaag acgagtgatg gaagttttgg acttggttgg attgaagcat
    17461 aaggttcgtt ctttcccaaa tgaactctca ggtggggagc aacagcggat tgcgattgcg
    17521 cgtgcaattg taaataatcc caaagtattg atagctgatg agccaacagg aaatctggat
    17581 ccggataatt catgggaaat tatgaatctc ttggaacgga ttaacctaca aggaacaact
    17641 attttgatgg cgactcataa tagccagatt gtaaatacct tgcgccaccg tgtcattgcc
    17701 attgaaaatg gccgtgtcgt tcgtgacgaa tcaaaaggag agtatggata cgatgattag
    17761 tagatttttt cgccatttat ttgaagcctt aaaaagtttg aaacgaaatg gttggatgac
    17821 agtagctgct gtcagttcag tcatgattac tttgaccttg gtggcaatat ttgcatctgt
    17881 tattttcaat acagcgaaac tagctacaga tattgaaaat aatgtccgtg tagtagttta
    17941 tatccgaaag gatgtggaag ataatagtca gacaattgaa aaagaaggtc aaactgttac
    18001 aaataatgac taccacaagg tatatgattc tttgaagaac atgtctacgg ttaaaagtgt
    18061 taccttttca agtaaagaag aacaatatga aaaattaacc gagataatgg gagataactg
    18121 gaaaatcttt gaaggagatg ccaatcctct ctatgatgcc tatattgtag aggcaaacac
    18181 tccaaatgat gtaaaaacta tagccgaaga tgctaaaaaa attgaaggtg tctctgaggt
    18241 tcaagatggc ggtgccaata cagaaagact cttcaagtta gcttcattta tccgtgtttg
    18301 gggactaggg attgccgctt tgttaatttt tatcgcagtt ttcttgattt caaataccat
    18361 tcgtattacc attatttccc gcagtcgcga aattcaaatc atgcgcttgg tcggagctaa
    18421 aaacagttat atccgtggac cgttcttgtt agaaggagcc tttatcggtt tattgggagc
    18481 tatcgcacca tctgttttgg tctttattgt ttatcaaatt gtttaccaat ctgtcaacaa
    18541 atcgttggta gggcaaaatc tatccatgat tagtccagat ttatttagtc cgttgatgat
    18601 tgccctacta tttgtgattg gggttttcat tggttcattg ggatcaggaa tatccatgcg
    18661 ccgattcttg aagatttagg taaaatagct gcttttatga ggagattgta aaatctcctt
    18721 ttttgctaca agagtttttg aaaagagatg cgcagaagaa aagagcttcc aaagaagtcc
    18781 cccagagaag acttcttgat ggctaatttg aacttgaaat ttggcgcact aga
//
LOCUS       DBERMR010000040        18666 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.040, whole genome shotgun sequence.
ACCESSION   DBERMR010000040 DBERMR010000000
VERSION     DBERMR010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 18666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18666
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.040"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(114..389)
                     /locus_tag="ACL8A1_001544"
     CDS             complement(114..389)
                     /locus_tag="ACL8A1_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HGS1202290.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(495..1334)
                     /locus_tag="ACL8A1_001545"
     CDS             complement(495..1334)
                     /locus_tag="ACL8A1_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HGS1202291.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRIGRVTGLLSSLLNIRAVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1476..2324
                     /locus_tag="ACL8A1_001546"
     CDS             1476..2324
                     /locus_tag="ACL8A1_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HGS1202292.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSGWLAFFEHIPLHIDLQ
                     DDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(2417..3334)
                     /locus_tag="ACL8A1_001547"
     CDS             complement(2417..3334)
                     /locus_tag="ACL8A1_001547"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HGS1202293.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVEQLKADEEVTRNWS"
     gene            3829..3962
                     /locus_tag="ACL8A1_001548"
                     /pseudo
     CDS             3829..3962
                     /locus_tag="ACL8A1_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
     gene            complement(4288..4581)
                     /gene="rpmA"
                     /locus_tag="ACL8A1_001549"
     CDS             complement(4288..4581)
                     /gene="rpmA"
                     /locus_tag="ACL8A1_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HGS1202294.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(4598..4942)
                     /locus_tag="ACL8A1_001550"
     CDS             complement(4598..4942)
                     /locus_tag="ACL8A1_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HGS1202295.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENS
                     SEFVQTRVITEN"
     gene            complement(4958..5272)
                     /gene="rplU"
                     /locus_tag="ACL8A1_001551"
     CDS             complement(4958..5272)
                     /gene="rplU"
                     /locus_tag="ACL8A1_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HGS1202296.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(5308..5395)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(5454..6157)
                     /locus_tag="ACL8A1_001552"
                     /pseudo
     CDS             complement(5454..6157)
                     /locus_tag="ACL8A1_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241810.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
     gene            6559..6858
                     /locus_tag="ACL8A1_001553"
     CDS             6559..6858
                     /locus_tag="ACL8A1_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HGS1202297.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
     gene            complement(7200..8174)
                     /gene="pta"
                     /locus_tag="ACL8A1_001554"
     CDS             complement(7200..8174)
                     /gene="pta"
                     /locus_tag="ACL8A1_001554"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HGS1202298.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(8218..9114)
                     /locus_tag="ACL8A1_001555"
     CDS             complement(8218..9114)
                     /locus_tag="ACL8A1_001555"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HGS1202299.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIYIPAEEGFETLEAIEHPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGNGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            complement(9111..9929)
                     /locus_tag="ACL8A1_001556"
     CDS             complement(9111..9929)
                     /locus_tag="ACL8A1_001556"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HGS1202300.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIECIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            complement(9913..10584)
                     /locus_tag="ACL8A1_001557"
     CDS             complement(9913..10584)
                     /locus_tag="ACL8A1_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HGS1202301.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            10712..11281
                     /locus_tag="ACL8A1_001558"
     CDS             10712..11281
                     /locus_tag="ACL8A1_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HGS1202302.1"
                     /translation="MKHLEIELKILLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            11394..12353
                     /locus_tag="ACL8A1_001559"
     CDS             11394..12353
                     /locus_tag="ACL8A1_001559"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HGS1202303.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            12363..13478
                     /locus_tag="ACL8A1_001560"
     CDS             12363..13478
                     /locus_tag="ACL8A1_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HGS1202304.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLKAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            13483..13830
                     /locus_tag="ACL8A1_001561"
     CDS             13483..13830
                     /locus_tag="ACL8A1_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HGS1202305.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            13838..14053
                     /locus_tag="ACL8A1_001562"
     CDS             13838..14053
                     /locus_tag="ACL8A1_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HGS1202306.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGKKVYQGLVGDLYRFLSHADFLHQN"
     gene            14136..14777
                     /locus_tag="ACL8A1_001563"
     CDS             14136..14777
                     /locus_tag="ACL8A1_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HGS1202307.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            14795..15484
                     /locus_tag="ACL8A1_001564"
     CDS             14795..15484
                     /locus_tag="ACL8A1_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HGS1202308.1"
                     /translation="MKKPVIGITGNEKTHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(15481..16161)
                     /gene="radC"
                     /locus_tag="ACL8A1_001565"
     CDS             complement(15481..16161)
                     /gene="radC"
                     /locus_tag="ACL8A1_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HGS1202309.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(16204..18495)
                     /gene="pcrA"
                     /locus_tag="ACL8A1_001566"
     CDS             complement(16204..18495)
                     /gene="pcrA"
                     /locus_tag="ACL8A1_001566"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HGS1202310.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGPGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KTAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDSDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKPASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
BASE COUNT         5724 a         3935 c         3407 g         5600 t
ORIGIN      
        1 acaatatttt cggaacattc aactttttaa ctctatttat tactagattt cataattaaa
       61 aagcctactg accaagctag aaagcttgat acaataggct ttttaaagac tgattattta
      121 acagcgtctt taagagcttt accagctttg aatgctggta ctttagaagc tgcaattgtc
      181 atttctttac cagtttgtgg gttgcgacct ttacgttctg cgcgctcacg aacttcaaag
      241 ttaccaaaac cgatcaattg aactttttca ccagctgcaa gatagtcagc tactgctgca
      301 aatacagctt caactgctgc tgctgagtct ttcttagtca attctgtagc ttctgctact
      361 ttagcgatca aatcttgttt gtttgccatg ttaatgattc ctccaaataa tttctaatta
      421 acaaatataa tcatatccta aaatcccttt taggtcaagt taaaaacgct atttcttccc
      481 attttattta ttttttagga gtggtaacgt atcaaaatag cccaagcgtt ctcacccgtg
      541 tgagtttgaa taatggaacc cgtttccaaa acagaaattg gcttttcaac ataagcttgt
      601 aagctttctt tcatctcttt tgcccaatca tcactaccag aatatgaaat tccaatctct
      661 gctacagcac gttcagaaag cgatgttatc aactcatcta accatttttt aaatgtttta
      721 gttccacgac ctttaaccat tggctgcaat tcatggtctt tcatttgcat gacagcacgg
      781 atattgagaa gagagctcaa caagccagtt acacggccaa ttcgtccacc tttgacaaga
      841 ttttccaaag ttgaaacacc aatataaagc tctgtatggt ttttaacctc ttctacatga
      901 gataaaattg cctccatatc tttaccttct tgagctaact tcgcagcctc aacaacttgg
      961 aatttcaggg cttggtcagt gaaggaacta tcaacaacag tcacatctgc agtagatagg
     1021 ctagcacctt ggcgtgctgc ttctaccgta cccgaaagag catgggacat atgaatagca
     1081 agaatctggc caccatcttt gcataggtct tcaaaaatct cagcaaagac acctacaggt
     1141 ggctgacttg ttttcggaag attcttactt tcttgcatca actgaagaaa tttaccttct
     1201 tctttcaaat ccgcatcaga ataaacaaca ttatcaatca ttacagataa tggaacaatt
     1261 gtaatatcta attgctttac tagttcaggt tcaatagtaa cagatgaatc ggttacaatc
     1321 ttaatttttg tcatagtatc aatctttcta ttttaggatt cagattggtt tccttacttc
     1381 taattatatc aaaaaaaaga ttaaaaatcc taatggagtc aatcaaattt tccgtaaaat
     1441 ttgatataat caacttataa gaaaagaggt gtcctatgat taaaaaaatt taccccattt
     1501 ttaccatttt actaggtgct gctatttatg cttttggact gacttatttt gtagttcccc
     1561 atcatctctt tgaaggaggg gcgacaggca ttaccctcat caccttttat ctttttaaaa
     1621 tccctgtttc cctcatgaac ctgctgatta atattcccct tttcatccta gcttggaaga
     1681 tttttggagc caaatccctc tattctagtt tactaggaac cttagctttg tccggctggt
     1741 tagctttttt tgagcatatt ccccttcata ttgatcttca agatgattta ctaatcacag
     1801 cccttatagc gggaatccta ttgggaattg gccttggaat tatttttaat gctggaggta
     1861 caactggcgg aactgatatt ctagctcgta ttctcaacaa atacactcat atatccatag
     1921 gaaaactgct ctttatctta gatttttgta ttctcatgtt gattctccta atcttcaagg
     1981 atttgagatt ggtttcctac acgcttttgt ttgattttat tgtttctcgt gttattgatt
     2041 tgattggtga aggaggatat gccggcaaag gctttatgat tatcacaaaa cgtcctgacc
     2101 aacttgctaa ggcgattaat gatgacctcg gaagaggtgt tacttttatt tctggtcaag
     2161 gctactatag taaagaaaat ttgaaaatca tttactgtat tgtcggaaga aatgaaattg
     2221 tgaaaacgaa ggaaatgatt catcgaatcg atcctcaagc ctttataact attacagaag
     2281 cccatgaaat cctaggagaa ggcttcacct ttgaaaaaga ataaaaagag gtagtgtcgt
     2341 gacctcaaaa gttagactaa atcatctatc ttttgggtta cagacaacct cttttttgtt
     2401 ttatttactc aagctcttaa gaccaattcc gagttacttc ttcatcagcc tttaactgtt
     2461 ccactaattg gtcaactgag tcaaatttgg tcatatcacg aatgcgatca agccaataaa
     2521 ccatgacggt ttccccataa atatcttgat taaaatcaaa aatattgact tcaaaacgtg
     2581 cttcttctcc atcaaaggtc acatttttcc cgacactagc catagcacga tacttctgtc
     2641 tttgaatctc aacatcaacg acataaacgc catctgctgg catataagta cggtctaaaa
     2701 gcactaaatt cgctgtcgga taaccaattg tacgaccacg agcattacca tgaaccacca
     2761 tacctcttga tggaagcggt gcccccaaaa gttttcctgc ttctttcaca tttccatcta
     2821 aaatagcttg acggatacga gttgaactaa tctttccttt ctcatcttct acaggtggaa
     2881 caatgataac ttctccatca aagtaatcct ttaaatcttc tgctgttttt ttgtcagaac
     2941 caaatgtata atcaaaacct gcaacaataa ttttggcatt catagccttg atataagttg
     3001 caaagaattc ttgtgcagtg agactagcga attgactact aaaatcaagg agatataatt
     3061 cttctacacc ttcgcgcttt aattttcttt cacgttcagc agggttcaaa atatgcaaaa
     3121 acaaatctgg atgataaggc tctaaagcga tctttggaga ttcattaaag gtcataacga
     3181 cgataggtaa taaatccttt ctcgcagcct tgttggcaac acgaaataat tcttgatgcc
     3241 ccttatgtat gccatcaaaa tagccgagaa caacgactga atcagatggt gtgccaatat
     3301 ctttttggtt ttttatagga atagtaataa tcataaaata attatatcat agcgatagct
     3361 atttctggaa cagaaaatct gaaatgttgt ttttttcaca tgaagtgtac ctgttttcaa
     3421 aaagcacttt attctatcgt tgcttaacta tgaactttgc aatattcttc tcaaaaactt
     3481 gtaggacatc ttcaaaattt tgcaaggagt gattagactt gttcggtaac cataaagtgt
     3541 catactatgc ttatgtatga aaaagcaatg caactaactc ctgagaactt taaattacta
     3601 attggtgccg aaaaggtaga atttagaatc gaggtacacc tatggctgta aaatttacaa
     3661 aatgagacaa cttgagcaag atgtttgaag aatttcctaa actccctgat ttgaagcaag
     3721 tcactttccc tgatgacaaa gaaaaaagcc aaaacagcaa agaaaaacta gatgactgct
     3781 ttccaacaac tcccatctag tgtgcttcag actaggctat ttttctctcc atctgttagc
     3841 ttggattctc agaccgtttc agctaaagaa tatcttttcc cttatcagaa ggaacggctc
     3901 aaaccattca gacaagtgaa gggacgacaa gccaatattt gaaaccagat agcagttctt
     3961 atagtcaatt gaaataaaat ctgaagaaat cgagtaggaa actcatatca atttttaaca
     4021 gtgttctatt ccagattcat actcaatgaa aattaaagtg caaactagga agttagccgc
     4081 aggctgtact tgagtacggc aaggtgaaac tgacgtggtt tgaatttgat tttcgaagag
     4141 tatcaatcca ctatacatat taaaaagtat ggatatcaaa acaaaaaaac aggctctccg
     4201 aaaactcgga aagccttatt ttaagctgtt tctagcttcc tcacctttac atttcaaggt
     4261 tcgggataaa aggttcaatg gaccttttta tttagcgatt gggtaaacag aaacttgttt
     4321 tttatcgcgt cctttacgtt caaagcgtac tacgccttca actttagcga acaaagtatc
     4381 gtctccacca cgaccaacgt ttacacctgg atagatgtgt gtaccacgtt gacggtaaag
     4441 gattgatcca cctgttacag tttgtccgtc agctgcttta gctccaagac gtttcgcttg
     4501 tgaatcacgt ccgtttgatg tagaacctcc accttttttg tgggcgaaaa gttgcaagtt
     4561 gttaagagtc atttttaaca taatgttttc ctccgtgtta gttttctgtg ataactctgg
     4621 tttggacgaa ctcagaagag ttctccgata agtttgccat acctaagaaa aatgattcaa
     4681 agaataactg ggtcatttct ctctggtgtg aaggaagatc ttttggtatt tcaaccatca
     4741 gatagccacc ttcatcttcg tttaattcta ggattggttc atagcctgca aatttctcaa
     4801 tagaattgat aaagttaatg gcaagcgtag aaaccgatgc acacacgaca tctaagccgt
     4861 attcgccact ctcggcgtgt ccagtaattt ccgcactcct cagctcgcca tcttcggctc
     4921 tctcaaagac tgcctgtatc atgtgttctc cttaaaatta agcgttgatt gcgttgatga
     4981 caacttttgt atatggttga cggtgacctt gtttacggtg gctacctttt ttaggtttgt
     5041 acttgtaagt aaccactttc ttttgttttc cttgtttttc aacagttcca actacagtag
     5101 ctccagcaac aagtggagtt ccgacaacag tgttttcacc accaacaaga acaacttcgt
     5161 taaaagtaac ttcttgacca gcttcaacgt tcaatttttc aacgtaaact gcttgaccaa
     5221 cttcaacttt aacttgtttt ccgccagttt tgataattgc gtatgtgctc attatgcacc
     5281 tcctatgatt tttatgggtt tccccgattt tttgtgaaga ctcgcctagc atcgtgggac
     5341 gaaccactta aacttgaata atcaagcaac gatgttcgtg cggttgcaca ggatcgtgca
     5401 tagtcaactc ttcaagtata gcatatctcc tattttctta caagtaataa cacctaaaat
     5461 gaagcttttt cttttacttt tttctgccaa gaggcaaaaa gcatgctgag gtaaaaaacg
     5521 ctcatcataa taggaacacc aagaatggtc ttttcatgat agaaaatcgt caaataggct
     5581 gaaaagacaa cgccaaggac aaaactacta agcaggctaa caaatatgaa tccttcacgc
     5641 aaaaaagagt gtgcttggtt cggaaataat ctccaaaagc cagcatggtc cgtttgatat
     5701 tccctgtcat aaaagcgtta ttataggcaa tacccgacac ttctccaaaa gcagttgtca
     5761 ccagtcccat acagaaggcc aagggcggca ctagatagat attatccaca gtttgcggca
     5821 caaaagcaat aatgattgat aagattgcca agggaatcaa ggacagaata ggttttttca
     5881 caattctcaa tttttcctta taaatcgtta ataaaaagac tcccatcata aacgctagca
     5941 aggtgagaac cttgtcccta acatccgaaa cattattttt aattaattct actgaaagaa
     6001 agacaacatt tccagtttgt ccagctacaa gggtattccc gcgaacaata aaagtgtaag
     6061 catccacata tccagcacaa aacgtcaaaa aaagtgctaa ccttttagac tgacgtgata
     6121 tttttcttat aggtaataac ctcattttac ctcccattgt attttctctt agaaatattg
     6181 taccattttc tttctaaaaa atcgtaggct accatttaga ttttactatt agcataaaaa
     6241 taataataga caactattta tccaaaaata gatagatgta acatgtttgc aaacaaagca
     6301 tacgaacctt tagtaaaatc atttccatga aactagaata gagccctctt agcaaaaatc
     6361 attattttaa tttatttcta atcactcctt gacataaata actctcacca ataaaagact
     6421 atgtcttaaa aaaatggtat aataaaatca atacttgggc ttgatggcta tgctactaat
     6481 aacaattagg agagaaaatc aggcacttgt taacaacaag gattatcccc ttgagatgaa
     6541 aggaacttta gaaatcttat gatgaacatg caaaacatga tgcgtcaagc acaaaaactt
     6601 caaaaacaaa tggaacaaag ccaagctgaa cttgctgcta tgcaatttgt tggcaaatct
     6661 gctcaagatc ttgtccaagc gaccttaact ggcgataaga aagttgtcag cattgatttc
     6721 aatccagctg tcgttgaccc agaggacctt gagactcttt ctgatatgac cgttcaagcc
     6781 atcaactctg ctcttgaaca aatcgatgaa actaccaaga aaaaactggg tgctttcgct
     6841 gggaaattgc cattctaaaa acaaggagct agaacaatac ttgtcgataa caaaggctaa
     6901 gaaaggtgca aaaattataa aaaacgcata atagcaggtg tcaagacact taatattatg
     6961 cgttttctca tgaaaaaatt ctcttttcat actcatttaa aatcaaaaag ggaatttatc
     7021 gttttacgga catgtatatt acagatttct ataggtcgtg tatagacagg atatcatcgc
     7081 ttggaagttt tcctttttga caccaatcag taatttagtt ctcaggaatt aatggcagtg
     7141 cttttcgtac atagtttcat aactacagta tagcatagta tatcacttta tagttttcac
     7201 tattgatgaa ctgcttgagc tgctgtgatg agggtcaact tgtaaacatc atctgcatta
     7261 catccacgag aaagatcatt aactggcttg tttaaacctt gcaaaacagg tcctacagcc
     7321 gcaaagccac ccaggcgttc agccatcttg taaccgatat ttcctgcctc gatacctggg
     7381 aagatgaaga catttgcttg accagctact gtacttcccg gagctttcag agctgcagtt
     7441 tcgggaacaa aggccgcatc aaattgcaac tcaccatcga tttcaaggtc aggacgcaag
     7501 tcgtgagcaa ttttagttgc ttcaacgacc ttatcaacgc tttcaccaaa ccctgaacct
     7561 ttagtagaat agctcaacat ggcaatttta ggttcgatgc caaacatctt agctgtgatt
     7621 gctgagttga tggcaatttc agccaaggct tctgcatctg gattgatatt aatggcacag
     7681 tctccaaata ggtaacgttc cgtaccacga accatgagga aggctcctga agtacgagtt
     7741 acatttggac gagttttgat gatttgtaga gctgggcgaa ctgttgaagc tgttgagtga
     7801 atcgctcctg acaccattcc atcaaccaag cccaagtaaa ccaacatcac accaaagtag
     7861 ttgacatctt caaccaaaac cttgcgtaca tcttcttcag tcattttgcc cttgcgacgc
     7921 tccaccaagg cagaaaccat ttcttcaaat tgaggataat gttgagggtc gatgacctca
     7981 taaccatcca tgattccttc aatttcaaga taaattttaa ttttttcagg atttccaagc
     8041 aaaacaggaa tcacttctgt ttcttttact aagcgttttg ttgcttgaag aatacgaggc
     8101 tcttcccctt cagggagaac gatacgagca tttttaccaa caaggttggc tttgagactt
     8161 tcaaaaactt ccatgagttt tctcctttaa gataataatt atactctgag acagttttta
     8221 tagagtatta gttgataact gggtaataag gttactgaaa tcatccggca agggactttc
     8281 taactgcaag tcttgctcta aaaatggatg ataaaaggat aggtaatggc aatgcagagc
     8341 ctgacgttga ataccatctt ccagactacc accatacaaa tcatctccca gcaaaggaaa
     8401 accgatatga gaaaaatgga ctcggatttg atgggttcga ccagtgtgca ggtgaatata
     8461 gaccaagtga atatttccat aagaagctac aatcttgtat gaagtatggg catactttcc
     8521 gcctttagcc actcgtctgg taataatgga atcttcatca cgcgcaatcg gagcaataat
     8581 ttccccttct ggctccaaat gtccatttcc cttaaccaaa gcaaagtagc gtttctcgat
     8641 agatttcttc tgcaactgct tgtctaatcg tgcatgggca taaccgtgct tggcaaagag
     8701 catcaagcca gaagtgtccc tatctagtct ggtaacaatg tgaacctgct gattttcata
     8761 attttgcttg acatagtaac ccttgataaa attggcaatg gtattagagt gattgacact
     8821 aggaatagaa gccactccat agggtttatt caagactaga aagtggtcat cctcatagag
     8881 aatatctaat ggatgctcaa tagcctccaa ggtttcaaag ccttcctcag cgggaatgta
     8941 aatggtaacg tagtctccaa cgtccaatag atacgttgca ttttgcggtt gattattgac
     9001 cagaatagct ccacctcgaa acttaatctt ggccagcaat cccttagaaa cctcgtgctt
     9061 ttttaaaaag gtcttaacct tgacatgttc atctgcgata aattcaaacc tcattcatcc
     9121 acctcaccga taaaggcatc cttaacacgg ttccagaaac tggtatggct aggagtcgcg
     9181 acaaagtgaa tcttatgatg gtcgatttga tactcaatac actcaatatt acggaaagaa
     9241 taaacgctat tgtcaaccga aatagtatga taatcgtttc ttgttggaat aagttcaatc
     9301 ttatccttct taggcacaat aatggaagag cccaatgttc gatagacacg attattaagg
     9361 ctggcaatct ccgttaattg caaagcttca atggtagggt gtaaaacagc accgccaaga
     9421 gacttgttat aggcagtact accagtcggt gtcgaaactg ttagcccgtc tccacgaaaa
     9481 cgttcaaagg gaacaccatt tattacaata tctgccacca tggttcgatc agacctgcgg
     9541 atgctggctt cgttgagtgc tctgaaaatc ttaacttcac cattttcgag aaagaccttc
     9601 acattcagaa cagggtaaga aacccttgcc ccagtatcta gctgcaaatt agtcactagc
     9661 ttgtccaact caaaatcacg ataatctgta tagaagccca aatgtccagt atgaagaccg
     9721 ataaagcgga ccttgtcaag ctgattttcg tacttatgaa aggccgacaa gagcatacca
     9781 tccccgccaa tggaaatgac aatatccgga ttggtatcat tgagtataaa ctgatttctc
     9841 ttcaaacgat ctcgcaattc atacaaaacc ctttgactct gcggttttct attggctatc
     9901 agatcaattc gtttacctgt attcttcatc tgtatcgtca ctgtttccta caccgtcatt
     9961 taattttcta ctcaaaggat caaaaagtgc ctgggcttct tggatatcat cacgaatttc
    10021 acccatttct tcatccaact gatgggcgat tctagctgta atttccagtc gcttcttaat
    10081 ctcatctggg aaatcccctt ggtacttgta gttgagagaa tgttctatcg ttgcccagaa
    10141 attcatggcc aaagtacgaa tttgaatttc tgccaaaata gtcttagctc cattgatggt
    10201 atcaaccgta tattctacta ccacatgata ggaacgatag cctgatgctt ttctatgagt
    10261 aatgtaatct cgctcctgta tgattcgcat atcctgacgc ttgtgcaaaa tatccactac
    10321 ttccttgacg tcatctacaa actgaaccat cacacgtaag ccagcaatat cctgcaaatc
    10381 gtgttccaag gtcgcataag taatgccacg acgagccatt ttttctttga tgctctcaat
    10441 tggcttgact cgaccggtca caaactcaat tggagaatgc ttattttgct tacgatattg
    10501 cttacgaata ccacgaagtt taatctttaa ctcaccaaca gcttgaatgt aaggatctag
    10561 aaattcttcc cattctaagg tcatatattc tcccttgttc tcatattatc cgtcaaaaat
    10621 atctttgatt ttttctccag attcatatac aataaataca atgcttatta taccataaat
    10681 ccgctttcaa cgataaaaaa aggtaagaaa aatgaaacat ttagaaattg aattgaaaat
    10741 actattgaaa aaagatgaat acaatcgtct aaaagaccag ttcacaggtg tcactcctgt
    10801 tcttcaaaca aattactaca tcgacacgcc tgattttgaa ctgcgagaaa agaaagtcgc
    10861 tatgcgcatt cgaacctttg aagactgggc tgagttgaca ctcaaagtcc cgcagagtgt
    10921 tggaaacatg gaatacaacc aaaaattgca actaaaagat gctgagaact atctgagtaa
    10981 ggaagaactt cctcaagggc tagtactgga tgaattggcg aaacatggta tccaaagtaa
    11041 gaactggcag gttcttggtt gtctaacaac gcttcgctat gaaatgaaaa cagctattgg
    11101 tctcatggca ctggatcaga gccgatactt tgatataaca gattacgaat tggagcttga
    11161 agtggaaaat catgagcaag gcaaacagga tttccgacaa tttttagaga aaaatcagat
    11221 ttcctaccaa aaagctcctt caaaattggt tcgatttgtc aaaagtatga aaaatagctg
    11281 aaataatctc cattttttgg taaaatagaa aagataaaaa tacacaaatc ccagttcata
    11341 tatgtaaggt aaatataact aggatttata aagtttacag aggacggtct ataatgtcag
    11401 atagaaaaaa catgaaactt ttcgcactca actctaacca agagattgca caaaaaattg
    11461 cccaagctgt tggtgtccca cttggaaaac tatcatcacg tcaattttca gacggagaaa
    11521 tccaagtaaa tatcgaagaa agtgtccgtg gttatgatgt ttacatcatc caatcaacaa
    11581 gtttccctgt caacaaccac ctaatggaat tgttaatcat ggtcgatgct tgtgtgcgtg
    11641 caagtgccca cagtatcaac gttgtccttc catattttgg ctatgcacgt caagaccgca
    11701 ttgcttgtcc tcgtgagcca cttacagcaa aactagttgc caatatgctg gttaaggctg
    11761 gagttgatcg tatcctgact cttgatttgc atgccgttca ggttcaaggt ttctttgata
    11821 ttccagtgga taatcttttc actgttcccc tattcgcaaa acattactgc gataaaggat
    11881 tgcttggttc agatgttgtt gtcgttagcc ctaaaaattc aggtgtcaaa cgtgcgcgta
    11941 gcctggctga atatcttgat gctcctatcg ccattatcga ctaccctcaa gacgatgcaa
    12001 ctcgtaacga aggttatatt attggtgatg ttgaaggtaa gaaagctatc ttgattgatg
    12061 atattttaaa tacaggacgt accttctctg aagcttctaa aatcgttgaa cgtgaaggag
    12121 ctacagaaat ttatgctgtt tctagccacg gtctcttcgt cgagggagct gctgagcttc
    12181 ttgacaatac taatattaaa gaaattcttg tgactgattc agtagcaaca aaagaaaaaa
    12241 ctcctaaaaa tgtatgctac atcactgcta gtgagttaat tggtgatgct atcgtccgta
    12301 ttcacgaaag aaaaccagtc agcccactct ttgcctacaa taaaaagaaa taaggtgatt
    12361 ctttgattta tttggacaat gctgcaacga ctcctatgtc agcagttgct atttcagcta
    12421 tgaccaaggt tatgcaagaa acccacggaa atccttctag tattcatggt catggtcgtc
    12481 aagctggcaa actcttgcga gaagcccgtc aggaactagc ccagttacta aggacaaaac
    12541 ctcaacatat ctttttcact tctggtggga ctgaaggcaa taatactacc atcattggct
    12601 actgtcttcg tcaccaagaa caaggaaaac atatcatcac aactgccatc gagcaccatg
    12661 ctgtccttga aacaattgat tacttggttc aacactttgg gtttgaagca accattatcc
    12721 agccagaaaa tcaagaaatc acagcccagc aaattcaaaa ggctttacgt gacgatacga
    12781 ttttggtttc taccatgttt gccaataatg agacaggaaa cctactgccc atcgctgaaa
    12841 ttggccaaat actcaagcaa caccctgctg cctatcatgt tgatgcagtt caggctattg
    12901 gtaaaatccc aattcattca gaagaattgg gcattgattt tctcactgct tctgcccaca
    12961 aattccatgg tcctaaggga atcggttttc tctacgcatc tagcatggac tttgattcct
    13021 atctacatgg cggagaccag gaacagaaaa aacgtgcagg aactgaaaat ctgcctgcta
    13081 tcgtaggcat ggttgcagcc ctaaaagaag acctagaaaa acaagaagaa cattttcaac
    13141 atgtacaaaa tctagaaact gcctttctgg cagagctgga gggtattcag tattacctga
    13201 atagaggaaa acatcatctc ccttatgttc tcaatattgg atttcctggt cagaaaaatg
    13261 acctcttact ccttcggcta gatttagctg gaatttcaat ctctactggc tcagcctgta
    13321 ctgcaggcgt tgtccaatcc agccatgttc ttaaagccat gtatggcgca aattcagaac
    13381 gcttgaagga atcccttcgc atcagtttgt cgccacaaaa taccgttgaa gacctacaaa
    13441 ccctcgcaaa aaccttaaaa gaaattatcg gaggttagcc atatggcatt tgaaaaaatc
    13501 attcagttaa aaaattgtcg ttacgattac actcttagcc cttctgttaa aaaattcacc
    13561 ctcaaagata acaccttttt tgaaactaag gttggtaact atgaactgac tcgccttttg
    13621 gaaaaagtgc caaacagcgg tgaaggcttc caactcaaaa tcatcattaa caaggaactt
    13681 acaggggcta aaatcaatat cactgacaag tttggccttc gtctagttga tattttcaaa
    13741 tcagaagacc accatattca tcaggaaaaa ttctacttcc tcatggatag cttggtagaa
    13801 cgtggtgtct ttacaaaatc ggaaagatag agccaaaatg tttgaactga cctataagga
    13861 ctgctatcat gtagagcgta ctctcaagta tgaggatcat gaggctctca tgttgacttt
    13921 gtcaggatgt gtaaccttac cagatacact ttatgtgact tctctgactt ttaggggcaa
    13981 aaaagtttat caggggctgg tcggagacct ctaccgtttt ctatctcatg cagatttttt
    14041 acatcaaaac taagattttt cttagttttt tcttattttt gttgattttt tcacaatgtt
    14101 tctataaaat agaagaatag aaaggttgtg attttgtgaa agataaacag tttgctattc
    14161 caaaagctac agcaaaaaga ctctctctct actatcgaat ttttaagaga tttcatgcag
    14221 aaaagattga acgtgccaac tctaagcaaa ttgcagaggc tatcggtatt gattcagcga
    14281 ccgtacgtcg tgatttttcc tattttggtg aacttggtcg tcgtggtttt ggctatgatg
    14341 tcaaaaaact aatgacattt tttgccgatt tgctcaatga caactctatt accaatgtca
    14401 tgctggttgg tattggaaat atgggccatg cccttctcca ctaccgcttc cacgaacgta
    14461 acaagatgaa gattatcatg gcctttgacc tagacgacca tcctgaagtc ggtacccaaa
    14521 ctcctgacgg gattcccatt tacgggattt ctcagatcaa ggataaaatc aaggatgctg
    14581 atgtgaagac tgctatccta actgttccca gcgtcaagtc acaagaggtt gctaatctct
    14641 tggttgatgc tggtgtgaaa ggaattctca gtttttcacc agtccatctg catttaccaa
    14701 aagacgtggt cgttcagtat gtcgatttga caagtgaact ccaaaccctc ctctacttca
    14761 tgcgaaaaga ggattagaaa gcgaaaatca ttttatgaaa aaaccagtta ttgggattac
    14821 aggaaacgaa aaaactcatc cagatgatga catcatgatg agctacgcag caaaaggctt
    14881 tgttgaaggc gttaaagatg ctggagggat tcccatcatc ctaccgattg gtgatcaaga
    14941 aatggcctgc cactatatca gtttgattga caagctcatc ttgacaggtg ggcaaaatgt
    15001 cgatccaaaa ttttatggcg aacccaaaac cattgatagt gatgactatc accttcaaag
    15061 agatatcttc gaactggccc tcatcaagga agctattaaa cagaaaaagc ccattttctc
    15121 tgtctgccgt ggtacccaac tctttaacgt tgccatgggt ggaactttgt accaagatat
    15181 cgaagaccat tggcaggatt cttctgttga gtacacaact caacgcttgg taacagaacc
    15241 agataccgtt ctccaagaaa tctatggaga aatctctcat atcaactcct tccaccacca
    15301 gagcatcaag gacttagcac caaatttaaa agttgtggct catgatccta aagatggtat
    15361 cattgaggct gtcatgagta cggatgatgt cgcctttctc ggtgtccaat ggcatccaga
    15421 acttcttttt gaaaatcgtc ccaaagataa aaaactcttt gactatgtcg ttaatgaact
    15481 ttagattaaa tctgtctttt cacgataact aaagtaatta gaatgagaga caatcaaatg
    15541 gtccaagaga acaatcccca tcaattcgca ggcttcttta acaagtttag tgacatgatc
    15601 atcattttgg ctaggcgcta ccgctcctga aggatgattg tggaccaaga taagagaagt
    15661 cgccatatgc ttgattgcat agtgaagaat ctctcgcggt tcagcgatac tacgagttac
    15721 agacccgata aaaatggtct gctgatggat gatttgattt tgagtattga gatagagtgc
    15781 caccaggtgc tcttgttttt tatcccctaa ttcctgctgc atcttcttgg ccaacttttg
    15841 actgctgaga atactttcca tttcaagagt ctcgtgtttg tgaatacgat gccccagttc
    15901 aatcatagct tgtaattcta tggccttaac acgcccaata ccagacaaac tctgcaattc
    15961 ctgcagggtc atttttttca aatccgttag gcttgaaaga ttgttcaaga ctttttgggc
    16021 aatttcaaaa acgctagctt gacgtgttcc tgtcctgagt aaaatagcta gcaactcttg
    16081 gttactaagc gcttcaactc cttccttggc cagcctttct cttggtaata gtgaatcttc
    16141 ttggaatgaa atactgtaca taaaaatcct cctcacttta ttattcgtga gaaggatgga
    16201 aaattagatt tttttctcaa ttggagccac actggctaaa agttttttca aacctacttc
    16261 tgggaaattg attttcaatt cctgcctagc accgctacct gaaacttcca gaacggttcc
    16321 ctctccccat ttcttgtgga gagcaatatc accaatggac caatttgcct cgctagatgc
    16381 tggttttgcg ccagctgtaa attgaccaaa tggaagaccg cttgactgga ttgattttgg
    16441 ggcagcaccg cgtttacggt cttgaagagc ctgagccaaa ctcatacctt gaccaaagga
    16501 aatactacca ctgctatatg atgccttaaa gcttgtattt gcaggacgag ccagaccttg
    16561 atactcaagc aagtctgaac tgatttcgtt aataaaacga gtcggacggt tataattggt
    16621 acgaccaaaa agcaagcgtg agttggcatt ggtcagatag agaattttct ctgcacgcgt
    16681 gatacctaca taggctagac ggcgctcttc ttctaattca tctgaatctt cagtcgcacg
    16741 actaagtgga aagacatttt cttccatccc aatcaaaaag acaactggaa attcgagacc
    16801 tttggcagca tgcagggtca tcaaggtcac ttctgatgtc tcctgactac ctgaatctgt
    16861 gtcggcaatc aaagccaagt catttaagaa acgactcagt ttgtccagac cagtttcctc
    16921 ttctgtcaca tccgtggtgt catcaaagtt cttcgtaaca gaaagaaact cttcgatatt
    16981 ttcaacccgt gccttgcttt ctagagtcgc ttgggcgtta agaatatcga cataacctgt
    17041 tttttctagg acggactcaa ccaactctgt aatgcttaag tggtctagct gctcccgcaa
    17101 atcaagcatc atattggcaa aatcccagat agattgggct gtcttaccct tgataccaga
    17161 caacataata ttagcagaag catccagcat agacatattt tgcaaatttg caaaatcacg
    17221 gattttctca actgtacctg gaccaattcc acgtttaggc tcgttgataa tacgctcaaa
    17281 actaatattg tcactcaaat tagcaataag gttgagataa gcaataatat cgcgaatttc
    17341 cttacggctg tagaatttgg ttccgccaac catggtataa ggaatgttag acttgagcag
    17401 ggcttcctca attgtacggg actgggcatt agtccgatag agaactgcaa aatccttatg
    17461 aaggaagttt tgactgcgac taagttcatc gatggttctg gctacaaata cagcctcatc
    17521 cagctcatca tcggcacgat agtaaacgat ttgctcccca tcagcgtttt gagtccagag
    17581 atttttagga cggcgatttt tattattttt aataacctcg ttggccgctt ggagaatggt
    17641 tttggttgag cggtaatttt cctccaacaa aacaacctta gctttggggt aatccttttc
    17701 aaagtccaag atattctgca tatcagcacc acgccaaccg tagatagact ggtccgcatc
    17761 cccaaccaca cagatatttt taaaacggga agccaagagt ttgaccaatt ggtactgagc
    17821 gtggttggta tcttggtact catcaacgtg gatgtattgg aatttttgct ggtagtaggt
    17881 caaaacatca ggattttgat caaagagacg caaggtcagc ataatcaaat catcaaagtc
    17941 aacggattca gactgacgaa gttctttttg ataggctgta taacactggg ccacaatttg
    18001 cgtatacata tcgccagctt gggcagcata agcaacatca tcaatcaaat cattcttagc
    18061 attggaaatg gtccccaaaa tagttcgttc attccatttt ttaggatcca agttcaactg
    18121 tttgagaata cgtttcatga gcgttcgctg ttcaccagga tccacaattg taaaattacg
    18181 attgtagcca atatggtccg catcgcgacg caaaatacgc acacacatgg agtggaaggt
    18241 cgcaatcaga cagtcctgag tcgctggatt gaggctataa gcacgctctt tcatctcacg
    18301 cgcagccttg ttggtaaagg taatggccaa gatattccaa ggattgacca gcttttcatc
    18361 aatcaaataa gcgatacggt gggtcaaaac acgagtcttt ccagaaccag cccctgccat
    18421 gattagcaag ggaccttctg tcgtttgcac cgcctcagcc tgacggtcat tcattccatt
    18481 taataatgcg ttcatcttct cttccttact cttgaagtca atatttctat tatatcagaa
    18541 atcagggaaa atagaaactt tacggggagt agcaaaatat agtggattga aactagaata
    18601 ctacactgtg ggtttctaaa acactcgact tttcacccaa ttttttcata aactagctca
    18661 atacct
//
LOCUS       DBERMR010000041        18650 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.041, whole genome shotgun sequence.
ACCESSION   DBERMR010000041 DBERMR010000000
VERSION     DBERMR010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 18650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18650
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.041"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(374..2254)
                     /locus_tag="ACL8A1_001567"
     CDS             complement(374..2254)
                     /locus_tag="ACL8A1_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HGS1202311.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIEGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDSLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDVL
                     CPILDRHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(2248..3117)
                     /locus_tag="ACL8A1_001568"
     CDS             complement(2248..3117)
                     /locus_tag="ACL8A1_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGS1202312.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILNKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGVSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAATGNALCQEAIERMNRNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVEDFVDAYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(3201..5846)
                     /locus_tag="ACL8A1_001569"
     CDS             complement(3201..5846)
                     /locus_tag="ACL8A1_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HGS1202313.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGEQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDDTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFALECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VAATGSLLSHSVLSIPQICPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTELIKKEEI"
     gene            complement(6037..7317)
                     /locus_tag="ACL8A1_001570"
     CDS             complement(6037..7317)
                     /locus_tag="ACL8A1_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HGS1202314.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDEIF
                     VVATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCDVNDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            7488..9572
                     /locus_tag="ACL8A1_001571"
     CDS             7488..9572
                     /locus_tag="ACL8A1_001571"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HGS1202315.1"
                     /translation="MKPLLETIDTRFGTTNKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGAWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGEDSLFHRQSSYNRDK
                     ASFQPHYLKIFSLRYQIETQLTPTCYGASIRLKQKQGKALSLYLHATDELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLVSSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKASAQADWENLLHRFDVIETGEANRTFFDHCLYRLFLFPQTF
                     YEVDESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            complement(9612..11291)
                     /locus_tag="ACL8A1_001572"
     CDS             complement(9612..11291)
                     /locus_tag="ACL8A1_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HGS1202316.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGACLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWANDAALPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGSGHTVGYKRLLRGAVVEAQKIRVVITESQVLPLLTKISLYKT
                     PRLSQTVAVQGLAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIQVLQFQAGESKKDLHLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            12046..13275
                     /gene="arcA"
                     /locus_tag="ACL8A1_001573"
     CDS             12046..13275
                     /gene="arcA"
                     /locus_tag="ACL8A1_001573"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HGS1202317.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESEYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELILSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            13333..14349
                     /gene="argF"
                     /locus_tag="ACL8A1_001574"
     CDS             13333..14349
                     /gene="argF"
                     /locus_tag="ACL8A1_001574"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HGS1202318.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            14514..15461
                     /gene="arcC"
                     /locus_tag="ACL8A1_001575"
     CDS             14514..15461
                     /gene="arcC"
                     /locus_tag="ACL8A1_001575"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HGS1202319.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVIVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            15670..17181
                     /locus_tag="ACL8A1_001576"
     CDS             15670..17181
                     /locus_tag="ACL8A1_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HGS1202320.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            17203..18534
                     /locus_tag="ACL8A1_001577"
     CDS             17203..18534
                     /locus_tag="ACL8A1_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HGS1202321.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNDLKEAGYDYQSTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVKLLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
BASE COUNT         5234 a         4228 c         3858 g         5330 t
ORIGIN      
        1 aaatgcatga gcatgatcat ttgcaaaatc gaaactccat aaaatcacat ctgctagctc
       61 cgctaacgtt tttgtttttg gaatctatat atgtcggtag caaccttttt taatttgacg
      121 gaaatgatcc aagcctctac agatgctggc ttttcagctt ctatttcttg ttttactaat
      181 tttaactgta gcatttgaaa ccagataatt cttctgtttc acgcgaaaca ccctgcgcca
      241 agcaaggtgt tcttgttttt atactcaatg aaaatcaaag agcaaactag gaagctagct
      301 gcaggctgct caaaacactg ttttgaggtt gtagatggaa gctgacgtgg tttgaagaga
      361 ttttcgaaga gtattaagtc gtataaatcg tcgacgctgt cgcaatggta tgccactggt
      421 tggctgttgt agctgcgaaa tccttgtctg cgtagaagtc agtaaatggt aggatttcaa
      481 cttccagctc gtcgatgcgg tcaagctggc cagccagata aacctcgata cggctttctg
      541 ctcgtttgat gcgttgcaag agtccgccca tacggatgtc aactgtatcc aaaccaaaga
      601 ccttgttttc tttcagccat tggtggctaa agagggcatg gaagtcttca atttggcttc
      661 taagttctgg taattcttgt ctggcgattt gttgtaaact ttctttatca tccgcttggt
      721 aggcctgacg aatgcgtcgt cccacatcta ctttgctact taaaatagca ttcaactggg
      781 cctgagtttc aaagagataa gcatagtttc cagctttttc tttaatgtta gcaagcgtct
      841 cagcagcctg agcgaagtgt ggcttgtcct gttcaggtgt catgtgtcgg tcaagtatcg
      901 gacagagaac atcctgataa aagacatatc ggttgggatt gatgccactg agattacctg
      961 gtaggtctgg taaaaggttg gcaaggtcaa tctgcataaa atcttcaacc gttagaccag
     1021 tattggtctt gaaatgcgca gacaaactat ctaggtcatt gcgatagctg agttctgccc
     1081 agatttgcaa gcttggtagg atagagaact gggcagtttc accaccattg tctccccaac
     1141 ccgttacgat gacttcttta atctgattgg cacggcaggc tttattagcc tcgatagcca
     1201 ctagacggct aaaatggttg tgaggtgtaa agccaatcca cttccaagct ccccctgcaa
     1261 atgcaaggtc atggctaatc ttgtgatgat tgcggaaatt acggttgtat ttttcctcgc
     1321 tatcctgata ataatcccag taaaccagag tcacacggtc tttgagacgg tctaggtaga
     1381 cacgagtttc ctctggaatt tccacatcac ggtcgtactg gccatccgct gacatgagtt
     1441 tgaagaacat atcactccac atctggcagt ggaaaccata tttgtcagca atatccagca
     1501 cgcgctccaa gtgttggcac atgaggagac tacgatccac aacaccgttc aaaatcaggt
     1561 agcgtcccaa accaaccaag tgggcttcgt ccatcccgat attgaccttg cgagtcttca
     1621 gtttagacaa cgtggcaaac atgccatcaa tcaggtcata aaccttttct tcgccaatga
     1681 gaagaatgtc ctctacatca cggagctcct gcacttcctt gacaccccat ttgacaaagg
     1741 ccgacaagtg ggccaaggtc tggatgcatg gtacaaaggt catgtcaaac tgttgagcat
     1801 aggcttcgat ttcctgcaac tcctctgctg aataagctcc acggaaatag ccaaagtaag
     1861 gctgcccttc aatctggtaa gtgtcttcca tgtaaagttc aaaggttgag tagcccatga
     1921 gagccaagac ctcaatcatc tgcttggcag aagccacatt cagcaccgca tttcgagaac
     1981 agtcaaccat gtaagccaaa tcttcgtaag ctgcttgttc ctcaatctct actttatcag
     2041 cttctgctag aactgttacc aacaaggata aggcacgata aagttggtga ggtttgcggt
     2101 aggtcaattg atagtgacct tcctcaccct cgatagagat agaggcttgg tcagactgag
     2161 tgacagccac ttccacatct ggtagagaaa tgtgaccttt taaaacctca atagcttgcg
     2221 tttgtttggg actaagtcct gtaaatctta ccattgcttt tcctcctgta gccagttgac
     2281 aagggcaccg tagagatttg catctgcgtg ataggtgcag gcctggataa ctggtgcgac
     2341 cgtgtattct tcgtaggcat ctacaaagtc ttcaacagcc ttcttaacac cttggataaa
     2401 atctggattt tgactgatag agccacccag actgatgaca tctggatcga tcagatactg
     2461 aatattgagc aagccttgcg ccagattgcg gttcatgcgc tcaatggctt cttgacaaag
     2521 agcattacca gttgcggcct cttggtaaat cttgcgaccg tcccaatcag tatgaccaga
     2581 tttttcaata acgtatcgta ccatattccc agttgatgct agttgcgacc agttattaag
     2641 tttttcagca ggggcaaggg ttgtcatgta accaaattct ccacccagac cgtggcgacc
     2701 tcgatgaagt ctaccattga taatcatggc tccgccaatc cctgtcccaa tcacgacaca
     2761 ggctgcattt tcaagctctg gatgagctag tagttcactg actccaacgc agttggcatc
     2821 attttctaaa tggacaggta gctgataaga gctaagcgcc tcataccaag aaaagccatg
     2881 gatgtagggc accgcactga agccatcaat cacacctgtc tcttgattga ccgcacctgg
     2941 aacgctcata gcaatcccac tgtaatcctg ttctgacaag cgttgatcta gccacgctag
     3001 taaatcctcc aagttttcag gcgttgaaat acttgtctta ttcagtattt ttccatcagg
     3061 agtcagactg gcaaacttaa ttccagtccc tccgatatca atcgttgcaa tggtcattgt
     3121 ttttaccata aaaaggggcg aatcagcagt tagttcttac tctttcgccc ctcctttctc
     3181 tttatgttta ctttttgaaa ttagatttct tcttttttaa tcaattctgt acgaatttct
     3241 tgtggagcca atagtcctga atgaactggg tatggtcgtt caagtaggtc aagaatagtt
     3301 tgttggtgtt ctgatatgcg cacattttct tgactcatat tgtagtaacg gagtacatat
     3361 ccttcttcat tttccgccac tttaaaggct gttggacaaa tttgcggtat gctgagaaca
     3421 gaatggctca agaggctacc agtcgcagcc acgcttcctt cctgtctagc aagctgaagg
     3481 ctggtaaacg gtgtctgcaa ggccttagct cgacgataag ctgagaagcg ttcttgggct
     3541 tggtggcact caagcgcaaa ctcgacttca aactcccgca agcattgtgc ttctggtgtt
     3601 gggaagtagc cccagtcacc tagctcacct gatgcacgca aaatagtcac tgcaatggtg
     3661 tcgtctccaa ggatttcgta ttcattcaat cccttgttgg atacagtcac acctttttca
     3721 tcgtcataca gactaacaaa ggcttgttgg tgttgaggat tttcaggatt ttcccatgaa
     3781 gcagctggtt tgtttggtcg tgtcaccacc tcatagatac tttcagaatc attgcttgga
     3841 cgcgtgttat gagtcttgac caagagacgg atacggtgat ccttggcagt gttagtaaag
     3901 cgagtcttga agcggatttg tggattgtca acgaagacag tcaactcagt ttccagagga
     3961 atgtttgtca attcttctga ccgtccagcc tcacgcttca taaactcgat gataccttgt
     4021 tgctcttctt ctagcttttc atccgcactg acaggcacgg tcaattcatg tttgagcaag
     4081 attttagcat agcgatctgt gttttccaag acctcgtggc ccttaagctc tgcaaagatt
     4141 ggctctgttc cttttggttg gaaatagata tactcgtttc cgatgtcacc ccggtcttcg
     4201 aagcggataa aatcttcata ggcttcgtga gttgtcttat catagactgt gatgttgtca
     4261 tccacactca ccgttacgaa tggtgtatca atcactccgt tttggtaaat accctcacgg
     4321 tgttcttgtt ctccttccag caattggaag gttgtccaag aaagcggtgc taggtgaact
     4381 ggaatggtca cgcgcacttg tcgagcaata cgagcttggc ggaacttatc ttttggtaaa
     4441 tcgtattcaa aattagctcc gaggtcttcg attgtagcct ctacaggacg accatccaag
     4501 tcctccacac ggtaacttgg caagataaga gcagccatct ttttgtagcc ttctgttggg
     4561 tgcaattcct tgaaatcaca agtcgccaca tcaatcactg tgctgacagt atcgacctta
     4621 tcatgcaagc ctgtgttaat gacagtaaag agatagtcac tttgagcctt atccgtagca
     4681 attttaccct tccactcgtt gagcaaatta cttttaacaa agtttcctac ttggttgacc
     4741 ttggcaaaac gcgtttccat ctcgcggtga acttcgtcca cgctacagcc acagatacta
     4801 tcatgtggcg cattctgcaa aagtgttttc caagcatagg tcaactggtc cttgtggttg
     4861 tgtccaccag tgataatagt caagggttct accacttgct ctaggaggtt gctattttct
     4921 tggaaggctt gttttaggta aatacgggat gaagaagtgt tggcaagtgt gtaccagcca
     4981 tctgtttcct gactggtcaa ctcgcctgta acagttgata agtgttcagg aagcgcacct
     5041 tctacagctt gaacatattc atcaaaagaa ctatgaacaa agattacatc cgggaagagt
     5101 tcatttgcca cacgaatggc ttcgctcaga tttttctgta caggctggtg gtcacagccg
     5161 ttcatcatca accattggtt ggtcgaagcg taggcacgca catctgacaa tttttgtttc
     5221 cagaaggtca aggcctcatc tttgtcaact gggatttcat tcccattact gtaccagttg
     5281 gcaaagagaa tacctaaaac acgactacca tccacaccct gccagtacat ttctgaaaac
     5341 tgggatgtaa actgctcatc ttcaaggact tggttgtcaa atccaatcgg cttcacacca
     5401 cgaccaaagg ccgccacgtg aatgcctgat ttttgaagaa tttgaggcgc ttgtcccata
     5461 tttccaaagg tatctggaaa gtagccaatc tgggttgatt taccccattt ggcagcttct
     5521 tgttgaccaa tcaaggtatt gcggacattg gcttcactgg agatcaaata atcatcctgc
     5581 aagatgtaaa agggaccaat tttaagcttg ccctcgtcaa tgtagcgttg gaccttatca
     5641 cgattttcag ggcgaatttg taagtagtcg tcaaggacaa tagtttgtcc atccaagtgg
     5701 aaactcttga actcagggtc attttcaaag agatcaaaga gattgtcaaa caattccacc
     5761 aactgcatac gatggctttc aaaaggcaag taccactcac ggtcccagtg actatgtgag
     5821 ataatatgta caacaacatt ttccatgaag taaaacctca ttctaaattt aaattttaac
     5881 gttttaaatg taaacttgtg attctgataa gaatcggttg agctaatact caacgaaaat
     5941 caaagtgcaa actaggaagc tagccgcagg ttgctcaaag cactgctttg aggttgtaga
     6001 taaagctgac gttgtttgag agattttcga agagtattag cgaatatcca agtaatccaa
     6061 gaccaactca cagaacatca tgttagccca ggagaaccat tcacgagagt agagggtcgg
     6121 atcatctacg tggaagcttt cgtgcatgac acctgtacca ccatcgcagg caaccagctg
     6181 atccagcaag aattttttct ctgccttatc tcttgttgtc aatccttgga tagaaagggc
     6241 gattggccag atatagcgat agaaggtatg agaacttccg agaccgctag cgtattctcc
     6301 ttggtagaag tatggatttt cagagctcaa aatggtacga cgagtagctt gatacacttc
     6361 atcgttgaca tcgcagtagc ccagataggg cgcagctagt agacttggta catttggatc
     6421 atccatgatg ctggcatttc ctaggccatc cacttcaaaa gcgtaaatct tttcgccctt
     6481 gctgttggtg gtgtaagcgt agttttcgat tccttcttgg atttcatcct gaagacgctt
     6541 ggcatcagca ataacactct ggctatcagc taggtttaat gctgcgaaga tttcttgcac
     6601 ataacccaag actactacag caaacatatt tgacggaatc aagtaactat actggcaaca
     6661 gtcatcactc ggacgaaagg ctgaccaagt catacctgtc actgcaaagt caggtccaaa
     6721 gccatcattt accaaggtgt cttccttacg gtccgtatct cggacaaaac gataaggaga
     6781 gttcttgtgg tcttgttcaa ctgtccatag atggagaatt tccttagtcg ctacgacaaa
     6841 aatctcatca aactgactag tctcgccagt ctctttccag aggagataag ccaactgcaa
     6901 aggatagcaa agtgaatcca cctcatactt gcgctcccag atccagccgt taaggtctgt
     6961 gtgatcagtc tcgtggtgcc ctttccagtt ctcctcaatg ttgaaggagt tagcataggg
     7021 atccttgagt accaaggtca tctgacgttt gaccaaacct gcaatggtct gacgcaggag
     7081 ggcatctctt ttagctacat gaaggtaggg tctgagttgg gctgtcgaat cccgaagcca
     7141 catggcagga atatccccag tcaagacaaa agttgaacca tcttctaaga tttcaaccgt
     7201 attgtccaag gtatcggtgt agcaacgctc gaaaacatcc acccactctg gatggtcctt
     7261 agcccgctct gctacttcat ctagccattc tctaacaatt tctttcgaat aaatcatcgt
     7321 gcaattccct ctttcaaaca agtttcatgt tcagtatata gcttttgaga cagaaaatac
     7381 atacacaaat gatagtcatt tttctacaaa atagttattt ttgaaactcc ttttctaaag
     7441 gccctctttt tctgatataa tgtagaaaaa cagctaaagg aaagactatg aaaccactac
     7501 ttgaaaccat cgatacccgc tttggaacta ccaacaagca tgccttttct cggggaaata
     7561 ccctgccata cacaggcgtg ccttttggga tgaattattt tgtgccccag accagtgacc
     7621 aggacggagc ttggttcttc gatccgcatc tgcctatctt tcaggggatt cgattaactc
     7681 accagcccag tccttggatt ggggactact cttggctcct tctgacacct gtcaccagtc
     7741 aactgggtga agacagtctc tttcaccgcc agtcttccta taatagggat aaggcctctt
     7801 tccaacctca ttatctaaag attttctccc tgcgctatca gattgaaacc cagctcacac
     7861 cgacttgcta cggtgcttct attcgtttga agcaaaagca aggcaaagcc ctctccctct
     7921 atcttcacgc aacagatgaa ctgacagtag agcaagtaga taagcgaact cttgccctgc
     7981 gacaagaagg taaaactgaa accaacaaaa attcgctaac gatgttcact gccctacaaa
     8041 tgaacacgga tattcttgct atcagccaag aagctggaga ctggcgaatt gacttagtaa
     8101 gtagtcaaac cgagatgcaa ctagccactt ctttcatctc tccttctcaa gctctaatca
     8161 atctacctca agaagatttt gatagctgta aagcaagtgc ccaagcggat tgggaaaatc
     8221 tcctccatcg ttttgatgtt atagagacag gagaggctaa ccgaaccttc tttgaccact
     8281 gtctctacag actcttcctc ttcccacaga ctttttatga ggttgatgag tcagggcaag
     8341 ccatccacat ggatctggct actggtactg tcaagcctgg tgtcctcttt agcaacaatg
     8401 gtttctggga taccttccgc accaccttcc ccctctttgc ccttatcata ccggagcact
     8461 atcaacgctt tttagaaggt ttcctcaata gctaccgcga tactggtttc cttccaaaat
     8521 ggctggctcc agatgaacgg ggtatgatgc caggtacact tttagacggt attatcgcag
     8581 atagcgcctg caaggacatg gcccctgacc tagaaggaga actcttccaa gccatgctcg
     8641 aaacagccag caaagccgac cctctcggca tcaatggccg ccacggacta gcccaatacc
     8701 aagaactagg ttacctctct accgaccacc acgaaagtgt tagccatact ctagactatg
     8761 cctatagtga cttttgtatc gctagctgtg ccaaaaaact agagaacata gaaatcgctg
     8821 aaacctacaa ggctgcttca caaaattacc gccagctatt tgacgctgag acaggttaca
     8881 tgcgagcacg agacaatcaa ggaaactttc accctgactt ctctccttat agttgggggc
     8941 gagactacgc tgaatgctct gccattcaag ctactttagg cgttctccac gacatccctg
     9001 gcttaatcca actgatgggt ggaaaagaaa cctttagcaa ctatcttttg aaagcttgtc
     9061 aagatgctcc cctctttgaa acaacaggct atggttacga aattcacgaa atgagcgaga
     9121 tggctactgc tccttttgga caaatagcca tttccaacca gcctagtttc cacattcctt
     9181 atctcttccg ctacagcgat taccctgact acactgccct tcttatcaag acgctccgtc
     9241 agaaagcttt tcacccaagt tgggaagcct atcctggaga tgaagacaat ggtagtctct
     9301 ctgcttggta catctggtca gctctcggat tttatccaac ctgtccagga aaaccaagct
     9361 atgacctcgg aatccctctc tttgaccatc tacgagtcta cctcgctaaa gaagataaat
     9421 ggctggatat ccatactaaa caaaaccaca accattttaa ctttgtcaaa gaatgccgac
     9481 tggacaaaac actcgtatca actattcaac accaagacct cttaaaagct gaacaactaa
     9541 ctttcaccct cagctggtta ccaagtcact aataaccaaa agaacctttg catcgcgcaa
     9601 aggttcttct tttatgaaac ttggacttga agttgatcca caagctgacc atccaccgtt
     9661 aggttcagat agaaatccaa ggttttatct cctgcgaaat acagggttgg tagatgcaaa
     9721 tcttttttgc tttcccctgc ttggaattga aggacttgaa tctcatcctg ataggcgaca
     9781 ccatggacac ctgtccccgg ttgaatcgaa atcttagcct ctaaagagga gctactttca
     9841 ctacgttcaa tcctaaaatg aagagtctct ccctttgcca cagctaggct tttttctgca
     9901 aatgctagcc cctgaacagc tactgtttgt gataacctcg gagttttata gagtgaaatc
     9961 ttggtcaaca aaggcaaaac ctgtgattct gtaatgacta cacgtatctt ctgtgcttca
    10021 acaactgctc ctcgtaagag acgtttgtaa ccaacagtat gacccgaacc aaactcctgc
    10081 cagacaccat ccacctctac ttgcacatga aaagcagcga ttcgttgccc tagcttcaaa
    10141 tcttctctta actcaattac atcaaaagtt ttaggagaac ctaagtcgag ttctaactgg
    10201 atgggcaagg ctgcatcgtt tgcccaagag ctggtctcaa ggccgtctgt caaatggcga
    10261 caagcaaagt ctgcggaaag agctggacca gatacctcag ctcccagagc caaatcttct
    10321 ttatagagct cattgcgata ggtcgcaaat tcataaagtc gttcaatatc ctttgcatca
    10381 aagagcccag cttgattcgg cggaatatta agcaagagtg gagttcctcg ccctactgag
    10441 tgaaagtaga tttcgaccaa ctcctcgaga gacttaggat cctgatcctc atggtagaac
    10501 cagcctggac ggatggaaac atctgcctct ccgattgaaa aaatcgtgcc cgagggatcc
    10561 ccgtgctgaa gatagttcag ctctgcttct gttcctagtt tatcaggatt caccttttgc
    10621 cacagtggat cacctgcata ccctcgttca ttgccaatcc agcggatact ggtgccttct
    10681 gttgaaaaaa tcaagcaagc gccctgcagg tcacgaatgg tttcaaacca tttttcaaat
    10741 tcataattaa ccttttgcgc gccctctcct ctggcaccat ccatccaaac ctcagcgaac
    10801 ttaccagcat tcccatagtt aggatttgat aagatttcct tcaactgagc cagataatag
    10861 gcattgtagt ccgcttctcg gtccacatga tagaggggac tatgggcatc ccacggtgac
    10921 aggtagaccc ccatatccat atcaaactct gtggcagctt gggatacttc aaggagcaag
    10981 tcgccctttc ctctcctcca aggactgacc ttaaccgaat aatctgtgtg agctgtcgga
    11041 taaaggacaa agccatcgtg gtgcttgacc accaaaatca actttttgaa gcccgtttcc
    11101 ttgagcacac gaacccactc acgcgcatcc aactgactcg ggttaaagcg ctcaggatcc
    11161 tcctgtccag tcccccattc ttggtcataa aaggtattag gaccgaagtg gataaaagct
    11221 gctagttcat ctcccagata agctagctga gtctgacttg gtaacggtcc atgcggttta
    11281 attttcttca tcgttatata tctccttctt tagcaacttc tcttgttcac aaggttatcc
    11341 acaggttgtg gataataaac catgatctaa ccgccagctc tttccaactc caactggtag
    11401 gctcaggatt tttctcctca atattccctc ttcaaacagc tctgtcatat ggttctgcta
    11461 acacctctca gaatgctact ctccataatt attctcaggc ttctttcagg tcattgtgtg
    11521 aagaccattc actctccaga cttttccaca gattgtgaat aagtgattat tctaaactat
    11581 cacctcgtcg actataacaa aaaataaaaa gagggtcaat aagataatta tctcttccta
    11641 aatactcaga aaagattcta taatttgtac aattgaacaa gaaatgcttc tccaaaatct
    11701 atcttcaaaa gaatgttgca tctttttatc ctttcacaca aacatttctc tttatacaga
    11761 tccaagtgat aaccagatga taattggaca ctttttggtc atttgtatat tttttgaaaa
    11821 attttttaaa aaaattttat atccttttta tagaagttta tgcatttgta tctatgtatt
    11881 ctatcctatg ttatgtataa taacataaaa ttgtgctaaa catcataatt tatattattt
    11941 tccgagaaat tatagccatt gacattacct aggaattgcg ttataacata catgtaagcg
    12001 gtacccaaaa tggtgccaag tcaaaatttt taaggaggaa aatacatgtc ttctcatcca
    12061 attcaggtct tctcagaaat tgggaaactg aaaaaagtta tgttgcaccg tccaggcaag
    12121 gagttagaaa acttgttgcc ggactatctt gaaaggcttc tttttgatga tattcctttc
    12181 ttggaagatg ctcaaaaaga acatgatgca tttgcccaag ctcttcgcga tgaaggaatt
    12241 gaggttctct acctagaaca actcgctgct gaatcattga cctctccaga aatccgcgat
    12301 caatttatcg aggaatactt agacgaagcc aacatccgtg atcgtcaaac caaggttgct
    12361 attcgtgaat tgcttcacgg catcaaggac aaccaagaat tggttgaaaa aacaatggct
    12421 gggattcaaa aagttgaatt gccagaaatt cctgacgaag ctaaagatct aactgactta
    12481 gttgaatcag agtatccatt tgcaattgac ccgatgccaa acctctattt cactcgcgac
    12541 ccatttgcaa caattggaaa cgccgtatcg cttaaccaca tgtttgcaga tactcgtaac
    12601 cgtgaaacac tctacggtaa gtatatcttc aaataccacc caatctatgg cggaaaagtg
    12661 gatttggtct acaaccgtga agaagatacg cgtatcgaag gtggagacga gctaattctt
    12721 tctaaagacg tccttgcagt aggtatctct caacgtacag acgcagcttc tatcgaaaaa
    12781 cttttggtca acatcttcaa gaaaaatgtt ggcttcaaga aagttttggc ctttgaattt
    12841 gctaacaacc gtaaattcat gcacttggat actgtcttca ctatggtaga ctatgacaag
    12901 ttcactattc acccagaaat cgaaggcgac cttcacgttt actcagttac ttacgaaaac
    12961 gaaaaactta aaatcgttga agagaaaggt gacttagctg aacttcttgc tcaaaacctt
    13021 ggtgtagaaa aagttcattt gattcgttgc ggtggtggca atatcgtagc agctgcgcgt
    13081 gaacaatgga acgacggttc taacactttg accatcgcac ctggtgtggt agttgtttat
    13141 gaccgcaata ccgtgaccaa taagattttg gaagaatacg ggcttcgctt gattaagatt
    13201 cgcggaagtg aattggttcg gggccgtggt ggacctcgtt gtatgtctat gccatttgaa
    13261 cgtgaagaag tgtaatcgct gttcggcatt cgtcaataga aaatgtaaaa aatagaaaga
    13321 ggaaataata aaatgacaaa ttcagtattc caaggacgca gcttcttagc agaaaaagac
    13381 tttacccgtg cagagttaga ataccttatt ggtctttcag ctcacttgaa agatttgaaa
    13441 aaacgcaata ttcaacacca ctaccttgct ggcaagaata tcgctctcct atttgaaaaa
    13501 acatctactc gtactcgtgc agcctttaca actgcggcta tcgaccttgg tgctcaccca
    13561 gaatacctcg gagcaaatga tattcagttg ggtaaaaaag aatctactga agatactgct
    13621 aaagtattgg gacgtatgtt tgacgggatt gaattccgcg gattcagcca acgtatggtt
    13681 gaagaattgg cagaattctc aggcgttcca gtatggaacg gtctaactga cgaatggcac
    13741 ccaactcaaa tgctcgctga ctacttgact gttcaagaaa acttcggtcg cttggaaggc
    13801 ttgacattgg tatactgtgg tgatggacgt aacaacgttg ccaacagctt gctcgtaaca
    13861 ggtgctatcc ttggtgtcaa tgttcacatc ttctcaccaa aagaactctt cccagaaaaa
    13921 gaaatcgttg aattggcaga aggatttgct aaagaaagtg gcgcacatgt tctcatcact
    13981 gaagatgctg atgaagcagt taaagatgca gacgttcttt acacagacgt ttgggtatca
    14041 atgggtgaag aagacaaatt cgcagaacgt gtagctcttc ttaaacctta ccaagtcaat
    14101 atggacttag ttaaaaaagc aggcaatgaa aacttgatct tcctacactg cttgccagca
    14161 ttccacgata ctcacactgt ttatggtaaa gacgttgctg aaaaatttgg tgtagaagaa
    14221 atggaagtaa cagacgaagt cttccgcagc aagtacgctc gccacttcga tcaagcagaa
    14281 aaccgtatgc acactatcaa agctgttatg gctgcgacac ttggtaacct ttatattcct
    14341 aaagtataat tttagataat aaaccgtcta ccaacagcta tgagggctgc gactaatagc
    14401 tttagtccgg tcctctttta tataatggta atctattatt tcttataaaa tatgtgaaaa
    14461 atcattaaat tgaaatctaa acgcattcta ttgagtgtga taaaggagaa tttatggcaa
    14521 atcgtaaaat tgtagtagct ttgggaggaa atgcgattct ttcttctgac ccatcagcaa
    14581 aggctcaaca agaagcttta gttgaaacag ctaagcatct tgtaaaattg attaaaaatg
    14641 gagatgattt gattatcact cacggtaatg gacctcaagt tgggaatctc ttgctccaac
    14701 atttggcatc agactctgaa aagaaccctg ccttcccact cgactcactt gtcgctatga
    14761 cagaaggtag catcggtttc tggttgaaaa atgctttgca aaatgctctc ttggatgaag
    14821 gcatcgaaaa aaatgttgcc tctgttgtaa cgcaagttat cgtagataaa aatgatccag
    14881 cttttgttaa cttgagtaaa ccaatcggtc ctttctattc agaagaagaa gcaaaagcag
    14941 aagccgaaaa gagcggagcg actttcaagg aagatgctgg ccgtggctgg cgtaaggtcg
    15001 ttgcctcacc aaaacctgtt gacatcaaag aaattgaaac catccgtact cttttaaata
    15061 atggtcaagt cgtcgtagct gcaggtggtg gcggtattcc cgtcgtcaaa gaaaacaatg
    15121 gacatttgac tggtgtcgaa gcggttattg ataaagactt cgcttcccaa cgtttggcag
    15181 aattggttga tgcagacctc ttcatcgttt tgacaggtgt agattatgta tttgttaact
    15241 acaacaagcc aaaccaggaa aaattggaac atgtgaatgt tgcccagctg gaagaatata
    15301 tcaaacaaga tcagtttgca ccaggtagca tgcttccaaa agtagaagca gctatcgctt
    15361 ttgtcaatgg tcgtccagaa ggaaaagcag ttattacttc ccttgaaaat ctaggcgcct
    15421 tgattgaatc tgaaagcgga acaattattg aaaaaggata agttgtttta ctaataagat
    15481 gtattctatt tctagtatct ttatatcaaa ttagaaatta ttcttgaaaa catgtacaat
    15541 atttcaaaag atactagttt tagactttaa tatggtaaaa caaatataaa tagaaagcgt
    15601 tttcttgaat gtttatttaa gaaagtagtt ggttttttac actttgttag acatcaggag
    15661 gaaaaacaaa tgagtgaaaa agctaaaaaa gggtttaaga tgccttcatc ttacaccgta
    15721 ttattgataa tcattgctat tatggcagtg ctaacttggt ttatccctgc gggggccttt
    15781 atagaaggta tttacgagac tcagcctcaa aatccacaag ggatttggga tgtcctcatg
    15841 gcaccgattc gggctatgct aggtactcat ccagaggaag gttcgctcat taaagaaacg
    15901 agcgcagcga ttgatgtagc cttcttcatc cttatggttg ggggtttcct tggcattgtc
    15961 aacaaaactg gtgctcttga cgtagggatt gcctctatcg tgaagaagta taagggccgc
    16021 gaaaaaatgt taattttggt actgatgcct ttgtttgccc tcggtggtac aacttatggt
    16081 atgggggaag aaacaatggc cttctatcca ctccttgtgc cagttatgat ggccgttggt
    16141 tttgatagcc tgactggtgt tgcaattatt ttgctcggtt ctcaaatcgg ctgtttggca
    16201 tctactctga atccatttgc gacaggtatt gcttcagcga ctgcgggagt tggtacaggg
    16261 gacggtatcg tacttcgtct gatcttctgg gttaccttga ctgctcttag tacttggttt
    16321 gtttaccgtt atgcggataa gattcaaaaa gatccgacta agtcactggt ttatagtact
    16381 cgcaaagaag atttgaaaca ctttaacgta gaagaatctt catctgtaga atctacactt
    16441 agcagcaaac aaaaatcagt tctcttctta tttgtgttga cattcatctt gatggtattg
    16501 agcttcattc catggacaga ccttggcgtt accatttttg atgactttaa tgcttggttg
    16561 actggtcttc cagttattgg taatattgtc ggttcatcta cttctgcact aggtacttgg
    16621 tacttcccag aaggcgcaat gctctttgcc tttatgggta tcctgattgg tgttatttat
    16681 ggtcttaaag aagataagat tatctcttcc ttcatgaatg gtgctgctga cttgctcagt
    16741 gttgccttga tcgtagcgat tgctcgtggt attcaagtta tcatgaacga cggtatgatt
    16801 accgatacaa tcctcaactg gggtaaagaa ggcttgagcg gtctatcttc acaagtcttt
    16861 atcgttgtaa cttatatctt ctatctacct atgtcattct tgatcccatc ttcatctggt
    16921 cttgccagcg caactatggg tatcatggct ccacttggag aatttgtaaa tgtccgtcct
    16981 agcttgatta tcactgctta ccaatctgct tcaggtgtct tgaacttgat tgcaccaaca
    17041 tctggtattg tgatgggagc tcttgcactt ggacgtatca acattggtac ttggtggaaa
    17101 ttcatgggca aactcgtagt cgctattatt gtagtgacca tcgcccttct tctccttgga
    17161 accttccttc cattcctata aaatagtgag tgaggtgatt ccatgaaaat agatataaca
    17221 aatcaagtta aagatgaatt tcttatatca ttaaaaacct tgatttccta tccttcagta
    17281 ctcaatgaag gagaaaatgg aacacctttt ggacaagcaa tccaagatgt cctagaaaaa
    17341 actttagaga tttgtcgaga cataggtttc actacctatc ttgaccctaa aggttattac
    17401 ggatatgcag aaatcggtca gggagcagag cttctggcca ttctctgtca tttggatgtt
    17461 gttccatcag gtgatgaagc agattggcag acaccgccat ttgaagcaac tatcaaagac
    17521 ggctgggtat tcggacgtgg tgtccaagat gataaaggtc cttcgcttgc agctctctat
    17581 gcagtaaaaa gcttgctgga ccaaggtatt cagttcaaaa agcgcgtacg ctttatcttt
    17641 ggtaccgatg aggaaaccct ctggcgctgc atggcacgct acaataccat cgaagaacag
    17701 gccagcatgg gctttgcacc tgactcatct tttcctctga cctatgctga aaaagggctt
    17761 ctacaggtca aacttcatgg ccctggatcg gatcaactag agcttgaagt aggaggcgcc
    17821 tttaacgttg taccagacaa ggccaactac caaggtcccc tctatgaaca ggtttgtaac
    17881 gatctcaaag aagctggtta tgattaccaa tccactgaac aaaccgtaac ggttctcgga
    17941 gtgccaaagc atgctaagga tgctagtcaa ggtatcaatg ctgtcatccg actagctacc
    18001 attcttgctc ctctccaaga acaccctgct ctcagctttc ttgcaacaca agcaggtcaa
    18061 gacggcacag gaagacaaat ctttggtgat atagcagatg aaccttctgg tcacctatcc
    18121 tttaatgtcg caggtctcat gatcaatcat gaacgttctg aaatccgtat tgacattcgg
    18181 actcctgtct tagctgacaa ggaagaacta gtaaagttgc ttacaagatg tgcacaaaac
    18241 taccaactcc gctacgaaga gtttgactat ctagcgcctc tatacgtcgc aaaagacagt
    18301 aaactcgtta gcacactgat gcaaatctac caagaaaaga ctggcgataa cagtcctgct
    18361 atttcatccg gtggtgccac ttttgctcgc accatgccaa attgtgtagc cttcggcgcc
    18421 ttattcccag gagcgaagca gacagaacat caggcaaatg aatgtgccgt tctagaagat
    18481 ttgtaccgtg ctatggatat ttatgccgaa gccgtctatc gacttgcaac ttaatcaggc
    18541 aactgtttct acaaaaaaaa atcgaccgat taatgaactg caccccaaaa gttagacaga
    18601 ataaatctaa cttttggggt gttttattat gaaattgagt tatgaagata
//
LOCUS       DBERMR010000042        17195 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.042, whole genome shotgun sequence.
ACCESSION   DBERMR010000042 DBERMR010000000
VERSION     DBERMR010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 17195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17195
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.042"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(401..1174)
                     /locus_tag="ACL8A1_001578"
     CDS             complement(401..1174)
                     /locus_tag="ACL8A1_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGS1202322.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTVPDQKLLDAVELFDGEWVSDIQ"
     gene            1350..2753
                     /locus_tag="ACL8A1_001579"
     CDS             1350..2753
                     /locus_tag="ACL8A1_001579"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HGS1202323.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLLQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGSTEGTAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            2868..3506
                     /locus_tag="ACL8A1_001580"
     CDS             2868..3506
                     /locus_tag="ACL8A1_001580"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HGS1202324.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMDLMAEKFKTYGQRK"
     gene            3518..3958
                     /locus_tag="ACL8A1_001581"
     CDS             3518..3958
                     /locus_tag="ACL8A1_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HGS1202325.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            3951..4382
                     /locus_tag="ACL8A1_001582"
     CDS             3951..4382
                     /locus_tag="ACL8A1_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGS1202326.1"
                     /translation="MFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVNELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISSYDSKINLEEIVQNAQNSLFNVKQQLNVEEEEDLCL"
     gene            4373..4843
                     /locus_tag="ACL8A1_001583"
     CDS             4373..4843
                     /locus_tag="ACL8A1_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS1202327.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            4868..5641
                     /locus_tag="ACL8A1_001584"
     CDS             4868..5641
                     /locus_tag="ACL8A1_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HGS1202328.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            5638..6438
                     /locus_tag="ACL8A1_001585"
     CDS             5638..6438
                     /locus_tag="ACL8A1_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGS1202329.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            6497..8791
                     /locus_tag="ACL8A1_001586"
     CDS             6497..8791
                     /locus_tag="ACL8A1_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HGS1202330.1"
                     /translation="MKLWYKKAASNWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKDCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPKTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQEREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            8801..11917
                     /locus_tag="ACL8A1_001587"
     CDS             8801..11917
                     /locus_tag="ACL8A1_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 98 C-terminal
                     domain-containing protein"
                     /protein_id="HGS1202331.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVPFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDPNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKRSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKGDSPSWWEIDLAQTEELEKLIIYNRTDAE
                     IQRLSNFDIIIYDSNNHEVFKQHIDSLESNNLSIDLKGLKGKKVRISLRNAGIPLSLA
                     EVEVYTYK"
     gene            11974..13740
                     /locus_tag="ACL8A1_001588"
     CDS             11974..13740
                     /locus_tag="ACL8A1_001588"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HGS1202332.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHTQKGIPAFGIYGRDVQEANDTAIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERALKWVKENVKEGFDHNREDLVLSREEKDRQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPIMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            13886..15037
                     /locus_tag="ACL8A1_001589"
     CDS             13886..15037
                     /locus_tag="ACL8A1_001589"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HGS1202333.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKVLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIVEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            15182..16066
                     /locus_tag="ACL8A1_001590"
     CDS             15182..16066
                     /locus_tag="ACL8A1_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGS1202334.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(16103..16261)
                     /locus_tag="ACL8A1_001591"
     CDS             complement(16103..16261)
                     /locus_tag="ACL8A1_001591"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202335.1"
                     /translation="MKIKEQTRKLATGCSKHRFKVVDKTDEVSSKHCFEVADRTDEVS
                     NIYTARRR"
     gene            16465..16716
                     /locus_tag="ACL8A1_001592"
     CDS             16465..16716
                     /locus_tag="ACL8A1_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HGS1202336.1"
                     /translation="MKEFQFERKQRFSLRPYAIGACSVLLGTSLFFDGMGDQPVQDTE
                     TSSALILSHYLDEQDLSEKLKSELQWLELENKLLNLWEH"
     gene            16704..16994
                     /locus_tag="ACL8A1_001593"
                     /pseudo
     CDS             16704..16994
                     /locus_tag="ACL8A1_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ZmpA/ZmpB/ZmpC family metallo-endopeptidase"
BASE COUNT         5697 a         2543 c         3644 g         5311 t
ORIGIN      
        1 tagatcatca aggtcactta attcttgaaa tatagggtct aggcgacaaa gtctttaaaa
       61 tcagaaaaac gcataatatc aggtgtgcaa aaacttgata ctatgcgttt tattgtggga
      121 aggtttactc cattttctcc tgaaattgag tttttgtcca gcctctgttt ttagggttgc
      181 tgagaaaata atgtcatgtg gtgaatattt gtaaatcagt cagcagacag aacgaatact
      241 cttcgaaaat ctcttcacat catgtcagct tcgtctttcc gtatatatgt gactgacttc
      301 atcagttcta tctacaacct caaaacagtg tttcgagctg acttcatcag ttttcaaatc
      361 tgtactttga gcaagctgag actagcttcc tatttgattt tcattgaata tcagaaaccc
      421 attctccatc aaataattcg actgcgtcta ataatttttg atctggcacg gtgtctgaaa
      481 taaaggttgt gtatttggag aggggattaa ttttaaaaaa tccagtcttg taaaatttag
      541 aactatcaat cagtaagatg gtttcatggg ctttgtcaat aatattcttt tttgaaatag
      601 cttggctgag agaagcttca taaacatatt ggtcatcaat acctcttgct gaacaaaatg
      661 ctaaatcgat attaaaatga tctaataaag aattttcctt atcatagttg accacggaac
      721 aggattgatg tttgacctcg ccagatgtga taaagatttt ggagctatct ttaacagttt
      781 cagatagggt ttgtgcagta tgtaaaccat ttgtaaaaat aatcaaatta tcaagttcag
      841 aaagataggg acaaagttcg tagacagtag tactagaatc tagatagata cacataccag
      901 accgaataaa gtctttagcg agactagcga ttagtctttt ttgcctagta ctttctcctt
      961 cccgtatttg atgagaaagt tcaattgtgt tcatagagga cagggtcacg tatccgtgct
     1021 ttcttttgat aagaccttga ttttctaaga aaattaaatc gcgacgtaag gtacttgtgc
     1081 tggagaaagt gatttctgcc agctctttta cggcaattct ttttttcttt ttgataattt
     1141 caatcaattc aagtacacgt tcatctttta tcataagctc ctcctaattt atcatttcaa
     1201 ctatattata gcacaaattg gaggaatttg aattattttt atgaatattg ggttaacatt
     1261 tgaacattat tcaagtaagc gttcacatat tgaaaaaata aaacgtgggg attataataa
     1321 agttaatcaa ggacgaagag agaagaaaaa tggaagcggt tttagcaata gatttaggtg
     1381 cgacttctgg aagagcaatc gttggttacc tttctgaaaa taaactagta atggaagaaa
     1441 taaatcgctt ttctaatcta cctattagag taaaagggca tttatcttgg gatattgact
     1501 ttctactagc taaaattctt gaaagtatcc gcttggctaa tactagttac aagattttat
     1561 ctatcggtat tgacacatgg ggagttgatt ttggactgat tgataatgaa ggtaagctgt
     1621 tattacaacc tgttcattat cgtgatgaaa gaacaaaggg agtgttaaag gaaatatctg
     1681 aaatgactga attagaaaaa ctgtattcag agacaggaaa tcagattatg gagataaata
     1741 ccttgtttca actctttaag gcacgtcaag aatctcctga ctctttctat aagaccaata
     1801 agattctttt aatgccagat ttgtttaatt atctcttgac aggtaagttt gctacagaaa
     1861 aaagcattgc ttcaacaact caattatttg atcctaggag tcaaaactgg aatcagaata
     1921 tcttaaaact atttgaattg gattcatctt tacttcctga gattgtttca gagggaaatg
     1981 ttcttggaag gataaaaaag gagtatggtt taggcgatat tcctgttgtg aatgtttgta
     2041 gtcatgatac agcaagtgcg attgtctcag tacctaagac agaaggtagt ttatttattt
     2101 catcaggtac ttggtctttg gttggagtgg aacttacttc accgattctt actaccgaat
     2161 ccttcagtta tggatttaca aatgaagtcg gtaaagatgg agtgattaca tttctgaaga
     2221 attgtacagg gttgtggatc atagaggaac taagacgttc atttgaacga agagggaaag
     2281 cctattcttt tgatgatatt aggacaatgg tggagaaaga aaaagaaaat cttcctctga
     2341 ttgatactga atcaactgaa tttgcaacag aatctgatat gcacaagact ttgacagaat
     2401 atctagctta tcatcatgaa actagagagt ggacagatgg acaactattt aagattgttt
     2461 atgaaagcct agctgaaacg tataggaaag cgatagagtt attagaagaa ctaactcata
     2521 aggtttataa gaggatatat gtgattggag gaggtgctag agccagttat tttaaccaaa
     2581 tgattgctga tagaactggt aaagaggttc ttacaggttc gactgagggt acagctgtgg
     2641 ggaatattgt tgtgcagctc atagctatgg gacaattaaa agggatggaa gaagctcacc
     2701 atgttattga ggagtttcta caattagaga gttattactc ccaaaagaat taaaaagatt
     2761 gagagtttgt aaatttgcct ccctccccct tcttaagttt tgtacaagaa ggggggataa
     2821 ttggtgaatt gaaaaatatt tagtgttttg atatgaggag gacaagaatg tcagatgtaa
     2881 aacaagaatt aattaagtat ggcaagaagc tagtagaaac agatttgacg aaaggaacag
     2941 gtgggaatct cagtgttttc gatcgtgaaa aacaattgat ggcaattacc ccgtcgggta
     3001 ttgatttctt tgaaatcaaa gaatccgata ttgtagtgat ggatattaat ggaaatgttg
     3061 tagagggaga acgcttgcca tctagcgaat ggtatatgca tttgattcaa tatcaaactc
     3121 gtgatgatat cgatgcaatt atccatgctc atacaactta tgcaacagta ttagcttgtc
     3181 tcagagaacc acttccggct agtcattata tgattgcagt ggcagggaaa gatgttcggg
     3241 tagctgagta tgcaacatat ggtacgaaag aattggctgt gaatgcagct aaagcaatgg
     3301 aaggtcgtag agcagtttta ctagcgaatc atggaatttt agcaggtgca caaaatttat
     3361 tgaatgcatt taatattgtt gaagaagttg aatattgtgc aaaaatttat tgtttagcta
     3421 agaattttgg agagccagta gttcttcctg atgaggagat ggatttaatg gcagaaaaat
     3481 ttaaaacata cggtcagaga aaatagggag gatattaatg ttaaaacata taccgaaaaa
     3541 tatttctcca gatttattga agactttaat ggaaatggga catggagatg aaatagtatt
     3601 agctgacgcg aattatcctt ctgcctcatg tgcaaataag ctaattcgtt gtgatggtgt
     3661 aaatattcca gaattattag attccattct gtatttaatg ccattagata gttacgtcga
     3721 tagttcaatt cagtttatga acgttgtttc gggtgatgat attcctaaga tatggggtac
     3781 ctatagacag atgattgaag gtcatggtac agatcttaaa acgattactt atcttagaag
     3841 agaagacttt tatgaacgta gtaagaaagc ttatgctatt gttgctacag gagaaacttc
     3901 actttatgct aatattatcc ttaagaaagg agtagttgtt gaaagagaaa atgtttaata
     3961 gaggaatttt agttgccagt catggtaatt ttgctagcgg agctctcatg accgcagaaa
     4021 tgtttgttgg tgagacaaca aatgatagag ttaggacatt aggtttgatg cctggagaga
     4081 atattgtaga gtttgaacat tattttaaaa atcaagtaaa tgaactgtta gactcaaatc
     4141 aagaggttat cgttttgact gacttgattg gaggaagtcc taataatgtg gctttgtcac
     4201 ggtttttaaa tttggattca gttgatattg taacagggtt taatatccct ctcctagtgg
     4261 aattaatatc aagttatgat tcaaaaatca atttagaaga aattgttcaa aatgctcaaa
     4321 atagtttgtt taatgttaaa caacaactta acgtagagga ggaagaggat ttatgtctat
     4381 agagtttgtt cgtattgatg accgtctggt acatggtcaa gttgtcacta cgtggctaaa
     4441 aaagtatgat attgagcaag ttatcattgt taatgatcgc atctcagaag ataaaacacg
     4501 acaatctatt ttaaagattt ctgcaccggt aggtttaaaa attgttttct ttagtgtaaa
     4561 acggtttgtg gaagttttaa actctgtgcc aataaaaaag agaacaatgc tgatatatac
     4621 aaatccaaaa gatgtgtatg attctattga aggaaattta aaattggagt acctcaatgt
     4681 aggacagatg agtaaaacgg aggaaaatga aaaggtaacg ggaggtgtag ctctaggtga
     4741 agaagacaaa tattatttta agaaaatagt tgataaggga acgagagttg aaattcaaat
     4801 ggttcctaat gataaagtta caatgttgga aaaattttta taaaaataat ttaaggaggt
     4861 acagtatatg ctattcacac aagcattact ggtgacatta gttgggatta ttgccactat
     4921 tgactataat ggaccgttat ttatgattca ccgtccgtta gttacaagtg caatggttgg
     4981 cttagtctta ggagatttca cccaaggtgt tcttattggt tcagctcttg aattaacttg
     5041 gctcggtgta acaggtattg gaggttacac tccaccagat actatttcag gtgcgattat
     5101 tggtactgca tttggtattt tgtctggtca aggagaaact gctggtatcg ctatagcagt
     5161 tccaattgca gttgctaccc aacagttgga tgttcttgca aaaactttag atgtttattt
     5221 tgtgaaaaaa gctgataatg atgctaaaaa cggagattat tcaaagatcg gtttttatca
     5281 ttattcaagt ttggttttaa tcactttatt taaaattgta ccaattttcc tagctattat
     5341 gcttggaggg gaatatgtgg cagacttgtt tgctaaggtt ccaccaatcg ttatgcaggg
     5401 acttaactct gcaggtgctt tactaccttc aattggtttt ggtatgcttt taaatatgat
     5461 gctcaagaaa aatatgtggg tattcttgtt gattggattc atttgttctg tgtatggagg
     5521 aatgtcaacc attgggatct cactagttgg tattgcggta gcatacttct acgatatgat
     5581 tggaagtaaa ccacaagaaa caacttcaag tagtgatgtt gaggaggatc ttgatctatg
     5641 atgaataata aagtaactaa agttgaactt aaaaaagttt tcaaacgaag ttttatgtat
     5701 ggttcttcat ggaactatga gagaatgcag aacctaggtt ttctatatac aattcttcca
     5761 gtattgaaaa aactataccc agacaaagat tcagcttctc ctgcaatgaa acgtcacctt
     5821 gagtttttca atactcatca aacagcggca ccatttattc ttggagttac ttccgctatg
     5881 gaagaacaag aaggaaatga aggtgcagct tcaattactg gtattaaagt tggcttgatg
     5941 gggccgctgg ctggtctagg agatagtttg ttctggctga cactagttcc tatctgtttt
     6001 agtattggtg cgtcttattc taaagacggc ggtgctttag gtatctttat cgccttaata
     6061 ttgtttaata ttattaatat tcctgttaaa tatttcggtt tgaaatatgg gtatactaag
     6121 ggttctagtc ttatccaaga aaataataca aaaggaacat tgaatcgcgt tacgagtatg
     6181 gcgacagcat tagggctagt actagtgggt ggtttgattc catcaatggt tggtattaat
     6241 tttggattag aatttaagca gggggaactt gttatttctg ttcaagaaat gattacaaaa
     6301 ttaattccag gatttatccc tatggctttg actttattaa tgtgtaaatt aattagaaaa
     6361 ggaaagaatc cggttgtact aatctttagt gttatggcta ttggagttat cctagttgtt
     6421 ttaggaattt tgaagtagta gaaagtgtgg aggtggtatt tgggatatca cctccatttt
     6481 ggaagagagg taaagagtga aattatggta taagaaagct gcctcaaatt ggaatgaagc
     6541 cttgccgatt gggaacggtc atttaggtgg tatgatttat ggttcagcta caaaagaatg
     6601 tattcaacta aacgatgaga ctatttggta tagaggaaag tcagatagaa ataatccaga
     6661 ctcactattg catcttaaaa aaattcggga atatctttta gatggagaaa ttcagaaagc
     6721 cgaagaattg ataaagttaa caatgtttgc taccccaaga gatcaaagcc actatgaatt
     6781 acttggggaa ctttacattg agcatataga tattcagtct tgtgctcttt cattgtatga
     6841 aagagagcta gatttagata cagctatttc taatgttgtg tttgagccta atagttgtaa
     6901 tttacaaata aaaagagaat attttacgag ttttaataag aatattttat gttgccgtat
     6961 agtgtcatca gttcaaaaca cattaaattt aaacattaat ttgggtagaa ataaacggtt
     7021 taatgacgaa gtatctaaac tggattcaag tacaatttta atgtcggcct ctgctggagg
     7081 tagaaaaggt gttcagttta aagtagtatg tcattctaaa gttacggatg gtgaagtaag
     7141 tgtattggga gagacaatag ttattcggaa tgctacagag gtatttcttt atctcaaatc
     7201 aatgacggat tattggggaa atatagatat ttcttctctt cagggagaat ttagtagtat
     7261 tgattacttt acagaaaaag atgaacatgt aaaaaaatat caggagcaat ttaatagagt
     7321 tgattttaaa ctagactata gtaaagattg tcttagcatt ccaacgaatc tacttcttga
     7381 aaacactaaa aagtatagta actacttgac taacttgtta tttcattatg gaagatatct
     7441 gttaatatcg tctagtcaac cgaatggttt acctgccaat cttcaaggaa tatggtgtga
     7501 tgaattaaat ccaatttggg gttctaaata tacgattaat attaatactc aaatgaatta
     7561 ttggatggta ggtccatgtg atttaccaga agtagaatat ccattatttg atatgctcga
     7621 aagaatgaga gaaccgggaa gactaaccgc taagaaaatg tatggagcta gaggttttac
     7681 agcacatcat aatacggatg gttttggcga tacggctccc caatctcatg ccatgggggc
     7741 tgcaatttgg gtattaacta ttccatggtt atgtactcat atttgggaac actatttata
     7801 tttccaagat gagcgtattc ttacggaaca ttttgaaatg ataaaagaag catttctttt
     7861 ctttgaagat tatttatttg aggtggatgg ctacttgatg acaggtccaa gtgtctcacc
     7921 agaaaataaa tatcgcttaa aaaatggtat tgaaggaaat gcttgtctat catctacaat
     7981 tgataatcaa attctaagat atttttgtga ttcatgcatt ggcattgcaa aacaattagg
     8041 agacaattcg gattttatta gtcgtgtgaa ggagttaaaa aagaaactac ctaaaacaaa
     8101 aataggtagt aatgggcaaa tccaagaatg gttagaagat tatgaagaag tagagcctgg
     8161 gcatagacac atttcacctc tatttgggct ttatccttat aatgagattg atattcataa
     8221 aactccggaa ttagcagaag cagctaaaat cactatcaat aggagattat caaacgctaa
     8281 ctttttatct tcacaggaga gggagcaagc gattaataat tggttagtaa gtggtttgca
     8341 tgctagtaca caaacaggtt ggagtgctgc atggctgatt catttttttg cgagactata
     8401 tcaaggtgaa cctgcttata accagattaa tggtttgtta aataatgcga ctcttggcaa
     8461 tttatttctt gaccatccac catttcaaat tgatggtaat ttaggtttgg tgagtggaat
     8521 ttgtgaatta ttagtacaga gccatcataa ttggttatca ctaattccag ctttaccttc
     8581 tgcttggtca gaaggagaag tgaaaggttt cagagtaaga ggaggatata aggtatcgtt
     8641 tgcttggaaa aatggggata taacattcct aaaattggaa ggaggaaaca aagatcaaaa
     8701 agtaagagta agaatatatg gcaaaaatac tgatgtacaa aatattgaat tggtatttaa
     8761 ttcagaaaaa attattgagt taaattttta ggtataagtc atgaataaag aaaaaataaa
     8821 aagaaaatta atcacaatac tgtttgtatg tattgggatg ttatgttttg gattgttagc
     8881 aggagttaag gctgataatc gtgttcaaat gagaacgacg attaataatg aatcgccatt
     8941 gttgctttct ccgttgtatg gcaatgataa tggtaacgga ttatggtggg ggaacacatt
     9001 gaagggagca tgggaagcta ttcctgaaga tgtaaaacca tatgcagcga ttgaacttca
     9061 tcctgcaaaa gtctgtaaac caacaagttg tattccacga gatacgaaag aattgagaga
     9121 atggtatgtc aagatgttgg aggaagctca aagtctaaac attccagttt tcttggttat
     9181 tatgtcggct ggagagcgta atacagttcc tccagagtgg ttagatgaac aattccaaaa
     9241 gtatagtgtg ttaaaaggtg ttttaaatat tgagaattat tggatttaca ataaccagtt
     9301 agctccgcat agtgctaaat atttggaagt ttgtgccaaa tatggagcgc attttatctg
     9361 gcatgatcat gaaaaatggt tctgggaaac tattatgaat gatccgacat tctttgaagc
     9421 gagtcaaaaa tatcataaaa atttggtgtt ggcaactaaa aatacgccaa taagagatga
     9481 tgcgggtaca gattctatcg ttagtggatt ttggttgagt ggcttatgtg ataactgggg
     9541 ctcatcaaca gatacatgga aatggtggga aaaacattat acaaacacat ttgaaactgg
     9601 aagagctagg gatatgagat cctatgcatc ggaaccagaa tcaatgattg ctatggaaat
     9661 gatgaatgta tatactgggg gaggcacagt ttataatttc gaatgtgccg cgtatacatt
     9721 tatgacaaat gatgtaccaa ctccagcatt tactaaaggt attattcctt tctttagaca
     9781 tgctatacaa aatccagctc caagtaagga agaagttgta aatagaacaa aagctgtatt
     9841 ttggaatgga gaaggtagga ttagttcatt aaacggattt tatcaaggac tttattcgaa
     9901 tgatgaaaca atgcctttat ataataatgg gagatatcat attcttcctg taatacatga
     9961 gaaaattgat aaggaaaaga tttcatctat attccctaat gcaaaaattt tgactaaaaa
    10021 tagtgaggaa ttgtctagta aagtcaacta tttaaactcg ctttatccaa aactttatga
    10081 aggagatggg tatgctcagc gtgtaggtaa ttcctggtat atttataata gtaatgctaa
    10141 tatcaataaa aatcagcaag taatgttgcc tatgtatact aataatacaa agtcgttatc
    10201 gttagatttg acgccacata cttacgctgt tgttaaagaa aatccaaata atttacatat
    10261 tttattgaac aattacagga cagataagac agctatgtgg gcattatcag gaaattttga
    10321 tgcatcaaaa agttggaaga aagaagaatt agagttagcg aactggataa gcaaaaatta
    10381 ttccatcaat cctgtagata atgactttag gacaacaaca cttacattaa aagggcatac
    10441 tggtcataaa cctcagataa atataagtgg cgataaaaat cattatactt atacagaaaa
    10501 ttgggatgag aatacccatg tttataccat tacggttaat cataatggaa tggtagagat
    10561 gtctataaat actgaaggga caggcccagt ccctttccca acaccagata aatttaatga
    10621 tggtaatttg aatatagcat atgcaaaacc aacaacacaa agttctgtag attacaatgg
    10681 agaccctaat agagctgtgg atggtaacag aaatggtaat tttaactctg gttcggtaac
    10741 acacactagg gcagataatc cttcttggtg ggaagtcgat ttgaaaaaaa tggataaagt
    10801 tgggcttgtt aaaatttata atcgcacaga tgctgagact caacgtctat ctaattttga
    10861 tgtgattcta tatgacaata atagaaacga agttgctaag aaacatgtta ataatttgtc
    10921 gggtgaatct gttagtctag atttcaaaga aaaaggagca aggtatatta aagttaaatt
    10981 actaacgagt ggagtgcctt tgagtttagc agaagtagag gtttttagag aatcagatgg
    11041 taagcgatct gaagaggata tagataaaat aacagaagat aaagtagtct ctacaaataa
    11101 ggtagctact caaagttcaa ccaattatga gggtgtagct gctttagcag ttgatggtaa
    11161 taaagatgga gattacggac atcattcggt gactcatact aaggcagatt ctaacgcttg
    11221 gtggcaggtc gatctgggag aagagtttac ggtttctaaa gttgatattt ataatagaac
    11281 agatgccgaa cctcagcgtt tatctaattt tgatgttatt tttctatctt catcaggaga
    11341 agaagttttt agaagacatt ttgataaagt agttgatggt ttgttatctt taaaagtacc
    11401 ttctgtaggg gctaagctag tcaaaataga attaaaatca gcagctattc cgttaagttt
    11461 agcggaagtt gaagtctatg gttcaaagag aactccgaag aaactttcta atattgcatt
    11521 aaccaaagaa actcgacaga gttcaacgga ttacaatggt ttttctcgtc tagcagttga
    11581 tggaaataaa aacggagatt atggtcatca ttcagtgact cataccaaag gagattctcc
    11641 ttcatggtgg gagatagatt tagcacaaac cgaagaatta gaaaagttaa ttatttataa
    11701 tagaacagat gctgaaattc agagattatc aaattttgat attattatat atgattcaaa
    11761 taatcatgaa gtttttaaac aacatattga cagtttagaa agcaataatc tatccataga
    11821 cttaaaaggg ctgaagggaa aaaaggttag aatttctttg agaaacgcag gaattccttt
    11881 aagtttggca gaggtagagg tttatactta taagtaattt taaaaattat catcggtagt
    11941 atgaaagaaa caaaaaaata agaggaaagt attatgatcc aacatccacg tattgggatt
    12001 cgtccgacta ttgatggtcg tcgtcaaggt gtacgcgaat cacttgaagt acaaacaatg
    12061 aacatggcta aaagtgtggc agatttgatt tcaagcacat tgaaatatcc agatggggaa
    12121 cctgtggaat gtgtgatttc tccatctacc attggtcgtg ttccagaggc tgcagcttcc
    12181 catgagttgt ttaaaaaatc aaatgtttgc gcaacaatta cagttacacc atgctggtgt
    12241 tatggtagtg aaactatgga tatgtctcca gatattcctc atgctatttg gggatttaat
    12301 gggacagaac gcccaggagc tgtctatctt gcagctgtac tagcttcaca tactcaaaaa
    12361 gggattccag cctttgggat ttatggtaga gatgttcagg aagctaatga tacagctatt
    12421 ccagaagatg tcaaagaaaa acttttacgt tatgcgcggg cagctcttgc aactggcttg
    12481 atgagagaca ctgcttacct atcaatgggt agtgtttcga tggggattgg tggttctatt
    12541 gtaaatccgg atttcttcca agaataccta ggaatgcgaa atgaatcggt agatatgacg
    12601 gagttcacgc gccgtatgga ccgtggtatt tacgaccctg aagagttcga acgtgcgctc
    12661 aaatgggtga aagaaaacgt aaaagaagga ttcgaccata accgtgaaga ccttgtttta
    12721 agccgtgaag aaaaagatag acaatgggaa tttgttatta agatgttcat gattggacgt
    12781 gacttaatgg ttggtaaccc aagacttgct gaacttggtt ttgaggaaga agcggttggt
    12841 caccatgctt tagtagctgg tttccaaggt caacgtcagt ggacagacca ttttccaaat
    12901 ggggacttta tggaaacttt cctcaatact cagtttgact ggaatggtat tcgaaaacca
    12961 tttgtatttg cgacagagaa tgattcacta aatggtgtgt ctatgctctt taattatcta
    13021 ttaacaaata ctccacaaat ctttgctgat gtgcgtactt attggagccc agaggctgtt
    13081 aaacgtgtaa cgggacatac tttagagggt cgtgctgcag ctggcttctt acatctaatc
    13141 aactctggtt cttgtacatt ggatggtaca ggtcaagcta ctcgagatgg caaacctatt
    13201 atgaaaccat tctgggagtt ggaagaaagt gaagtgcagg ctatgcttga aaatacagac
    13261 ttcccaccag caaaccgcga atacttccgt ggaggaggat tctcaactcg tttcttgacg
    13321 aagggggata tgccagtaac aatggtacgt ctcaatcttc taaaaggggt tggtccagtg
    13381 ctacaaattg cagaaggtta tacacttgaa cttcctgaag atgttcacca tactttagat
    13441 aatcgtacag atccaggatg gccaactact tggtttgctc cacgtttgac aggaaaaggt
    13501 gctttcaagt ctgtctatga cgtcatgaat aattggggag ctaatcacgg agccataaca
    13561 tatggacaca ttggagcaga cttgattacc ttggcttcta tgttgagaat tcctgtcaat
    13621 atgcataatg tacccgagga agatatcttt agacctaaaa attggtcctt atttggaaca
    13681 gaagatctag aatcagcaga ctatcgtgca tgtcagttgt tgggaccgct acataaataa
    13741 aaattgtgta tataggaggt gaacttatgt ccctcctatc cttttaaaaa gatttgttaa
    13801 acaattcaca aataattgaa aacgaataca aaaactaata taatgatgtt aaatagatag
    13861 cgcggaggcg caggaggaaa attatatggc tacattttat gttccggcag tcaaccttat
    13921 tggtaaaggt gttgtaaatg aagtgggtcc ttatatcaag gaacttggct ataaaaaggc
    13981 acttttggtg acagataagt acatcgaagg cagtgacatt ttacctaagg ttttaaaacc
    14041 actggataca gaaggaatcg aatatgtcat ctttagcgat gtagagccaa accctacttg
    14101 taaaaatgtc acagatgggg tagctgcttt gcaagaacat ggctgtgact ttatcatcag
    14161 tcttggcggg ggttctccac aggatgcagc tagttgtatt tctatcatgg ctacaaatgg
    14221 tggaaaacca caggattatg aagggcttca taagtctgct aaaaaaggct tgccagttgt
    14281 ggctatcaat acaacggcag gaacttctgc agaaatcact attaactatg tgattactga
    14341 tgaagaacgc aaggttaaga tggtaatggt tgacaagaat agccttgctc ttatctctgt
    14401 taatgacccg gaactcatgc tttccaaacc taaaggcctg actgctgcta ctggtatgga
    14461 tgctctgact cacgctgttg aagctttggt aacacctggt gcttatgatg taaccaagaa
    14521 actgtctatt ggtgctattg agcttatcaa ggaatatctt cctcgtgctg tagaaaatgg
    14581 acatgatatt gaagcgcgtg aaggtatggt caatgccatc ttccttggtg gtatgagctt
    14641 taataatgct ggtcttggtt atgttcactc aatggctcac caactcggtg cagtatataa
    14701 tttgccacat ggtgtttgct gtgccatgtt gctaccagtt atagaacgtg aaaatgctaa
    14761 acgtgtacca gaagctttcc gcaatgttgc caaagccttg ggacttcatg tagaaggtaa
    14821 atcagatcaa gaatgtgccg attatgcgat tgttgagatt gagaagcttt ctgagacagt
    14881 aggtattcct aagaaactga ctgaacttgg tattgaagaa aaagatttcg actttgaata
    14941 cctttctaag aatgccttga ttgatgcctg cgcaccagga aatccattta tgccaacctt
    15001 agaagaaacg attgcctttt ataaagagtt attttagcaa gagattggac gacaatagga
    15061 caattcacct ctaattattg acatttcagg aaaaatcgca gtaaaaacgg cacagccttc
    15121 tttaaatgtg ctataatact agaaaaatac ttgtggaggt tccattatgg caatattttt
    15181 catgattttt ctgattgttt gtgtgctcct attggtgata gtcacactga gtacagttta
    15241 tgtggttcgt cagcagtcgg tggcgattat tgaacgcttt gggaaatacc aaaaggttgc
    15301 taatagcggt attcatattc gcttgccttt tgggattgac tcgattgcag cacggattca
    15361 gctgcgcttg ttgcaaagtg atattgtggt tgagactaag accaaggaca atgtgttcgt
    15421 tatgatgaat gtagcgactc agtaccgtgt caacgagcag agcgtgacag atgcttacta
    15481 taaactcata cgtccagaat ctcagattaa atcttatatc gaagatgctc ttcgctcttc
    15541 tgttccaaaa ttaaccttgg atgaattgtt tgagaaaaaa gatgagattg cccttgaggt
    15601 tcaacaccaa gtagcagaag aaatgaccac ttacggctac attatcgtga aaaccttgat
    15661 taccaaagtc gaaccggatg cagaagttaa gcaatccatg aatgaaatca atgcggcgca
    15721 acgtaagcgg gtcgcagcac aagaattggc ggaagctgac aagattaaaa ttgtcactgc
    15781 agctgaagcc gaagcagaaa aagaccgcct tcatggtgtg gggattgccc aacaacgtaa
    15841 ggcgattgtg gatggattgg cagagtctat caccgaactc aaggaagcca atgttggcat
    15901 gacagaagaa caaatcatgt ctatcctctt gactaaccag tatttggata ccttgaatac
    15961 ctttgcctct aaaggaaatc aaaccatctt tttaccaaat acgccaaatg gtgtggatga
    16021 tatccgaaca caaatcttgt cagcccttcg cgctgagaag aaataataga ctaatactct
    16081 tcgaaaatct cttcaaacca cgtcagcgtc gccttgccgt atagatgtta ctgacttcgt
    16141 cagttctatc tgcaacctca aaacagtgtt ttgagctgac ttcgtcagtc ttatctacta
    16201 ccttaaaacg gtgttttgag caacctgtgg ctagtttcct agtttgctct ttgattttca
    16261 ttgagtacaa agagcttggc aacaggctct ttttgtattg cgtttattaa gaaaaacaaa
    16321 aaacatcgat atacatttaa ctaaaaatta ttgtatgtta tctctcttaa ttaggaatga
    16381 taagggaata actagaaaga tttgtgaata caaactattt ctgatataca aaatatacag
    16441 taataatgaa tgatgggaga tgggatgaaa gaatttcaat ttgagagaaa gcagcgtttt
    16501 tctttgaggc catatgcaat aggagcttgt tcggtcttgc taggaacgag tttatttttt
    16561 gatggtatgg gtgatcagcc tgtacaggat acagaaacga gttcagcact aattttaagt
    16621 cattatttgg atgagcagga tttatctgaa aagctgaaat ctgagttgca atggcttgaa
    16681 ttagaaaaca agcttttgaa cttatgggag cattaggtta ctatgaagga tttgttcctt
    16741 atgtttcaaa ttaatacaaa aaccaagctg aagaagaaga caaaccgcta tctgataaat
    16801 atattttcga aaaaatatta ggaaaaacat atgcagcctt caaaaaagat caaattaatg
    16861 agcgtgtcga gaaattaggt aagttaaaac ctattacaat aaattacaac ggaaaatcag
    16921 aagtaattga tagtaaagaa aaattacaag agcttatgaa taaagccgtt aaagacgaag
    16981 tggctcaaat atagtaaaat gaaataagaa caggacaaat caatcaggac agtcaaattg
    17041 atttctaaaa atgttttaga agtagaggtg tactattcta gtttcaacat actataaaat
    17101 caggcaatac aacggctaaa aagtttgagt ttatcgaaat accagtgcaa aaactgaaaa
    17161 aagcgattta taaagctcat ctcaaagatt cagat
//
LOCUS       DBERMR010000043        17127 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.043, whole genome shotgun sequence.
ACCESSION   DBERMR010000043 DBERMR010000000
VERSION     DBERMR010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 17127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17127
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.043"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(93..323)
                     /locus_tag="ACL8A1_001594"
     CDS             complement(93..323)
                     /locus_tag="ACL8A1_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202337.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            complement(341..982)
                     /locus_tag="ACL8A1_001595"
     CDS             complement(341..982)
                     /locus_tag="ACL8A1_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HGS1202338.1"
                     /translation="MKLLKNLGWILLALLSFLFIYGFIQGLATASLALGASPYAVTLL
                     YVALAVVYVYGIYKWYQKAPVHIEKSGFNRFIWLPVLVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            complement(994..1728)
                     /locus_tag="ACL8A1_001596"
     CDS             complement(994..1728)
                     /locus_tag="ACL8A1_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HGS1202339.1"
                     /translation="MKKKRRLLFLMSIVLGGFLGMFVGMFKARVESHEIILDVKALMP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NITGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIILSFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            complement(1731..1928)
                     /locus_tag="ACL8A1_001597"
     CDS             complement(1731..1928)
                     /locus_tag="ACL8A1_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202340.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            complement(1928..2134)
                     /locus_tag="ACL8A1_001598"
     CDS             complement(1928..2134)
                     /locus_tag="ACL8A1_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS1202341.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            complement(2144..2608)
                     /locus_tag="ACL8A1_001599"
     CDS             complement(2144..2608)
                     /locus_tag="ACL8A1_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202342.1"
                     /translation="MIDKVVRNLLLTFFFCKMTKIINFLTAILVKKKKMCYNVSKLRE
                     KKKGAMMWVLVFILFIIFFYSNNSKKIKKLENKIKKLERKEKGNAEMSRLLQEMIGKK
                     PIITGVYIGPDNWEVVDVDEEWVKLRRVDNTGKEKFKLQRIEDIQTVEFDGE"
     gene            complement(2713..4002)
                     /gene="hisS"
                     /locus_tag="ACL8A1_001600"
     CDS             complement(2713..4002)
                     /gene="hisS"
                     /locus_tag="ACL8A1_001600"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HGS1202343.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIAMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQTHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     RQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(4080..5286)
                     /locus_tag="ACL8A1_001601"
                     /pseudo
     CDS             complement(4080..5286)
                     /locus_tag="ACL8A1_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
     gene            complement(5477..6328)
                     /gene="rgg"
                     /locus_tag="ACL8A1_001602"
     CDS             complement(5477..6328)
                     /gene="rgg"
                     /locus_tag="ACL8A1_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398326.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HGS1202344.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIIIKSYMKSIDERVELTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRKTLDS"
     gene            6452..6793
                     /locus_tag="ACL8A1_001603"
     CDS             6452..6793
                     /locus_tag="ACL8A1_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HGS1202345.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            6857..7156
                     /locus_tag="ACL8A1_001604"
     CDS             6857..7156
                     /locus_tag="ACL8A1_001604"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202346.1"
                     /translation="MKIKGQTRKLAASCSKHCFEVMDRTDEVSSKYCFEVVDRTDEVS
                     SKHCFEVVDRTDEVSSKHCFEVADRTDEVSSKHCFEVVDRTDEVSNHTYGKATLT"
     gene            complement(7182..8885)
                     /gene="ilvD"
                     /locus_tag="ACL8A1_001605"
     CDS             complement(7182..8885)
                     /gene="ilvD"
                     /locus_tag="ACL8A1_001605"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HGS1202347.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETPIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(9118..10050)
                     /locus_tag="ACL8A1_001606"
     CDS             complement(9118..10050)
                     /locus_tag="ACL8A1_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HGS1202348.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSDSDIVVLEADLSSSMATHNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFSSRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEFYNAR"
     gene            complement(10047..10904)
                     /locus_tag="ACL8A1_001607"
     CDS             complement(10047..10904)
                     /locus_tag="ACL8A1_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGS1202349.1"
                     /translation="MILSKNREDGLRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEERQMLTSVVER
                     LSQELEETE"
     gene            complement(10908..12254)
                     /locus_tag="ACL8A1_001608"
     CDS             complement(10908..12254)
                     /locus_tag="ACL8A1_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGS1202350.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFVGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGILNQFGSQAGIVIGLVLILAVMFGVSFIKKPSATEE"
     gene            complement(12267..12551)
                     /locus_tag="ACL8A1_001609"
     CDS             complement(12267..12551)
                     /locus_tag="ACL8A1_001609"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS1202351.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKICEEKGLI"
     gene            complement(12555..14585)
                     /locus_tag="ACL8A1_001610"
     CDS             complement(12555..14585)
                     /locus_tag="ACL8A1_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGS1202352.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            14776..15783
                     /locus_tag="ACL8A1_001611"
     CDS             14776..15783
                     /locus_tag="ACL8A1_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGS1202353.1"
                     /translation="MTEKLINSKPNGVFALILIELTIILGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKHISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            15786..15986
                     /locus_tag="ACL8A1_001612"
     CDS             15786..15986
                     /locus_tag="ACL8A1_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGS1202354.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            16138..16320
                     /gene="rpmF"
                     /locus_tag="ACL8A1_001613"
     CDS             16138..16320
                     /gene="rpmF"
                     /locus_tag="ACL8A1_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HGS1202355.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            16336..16485
                     /gene="rpmG"
                     /locus_tag="ACL8A1_001614"
     CDS             16336..16485
                     /gene="rpmG"
                     /locus_tag="ACL8A1_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HGS1202356.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(16977..>17127)
                     /locus_tag="ACL8A1_001615"
     CDS             complement(16977..>17127)
                     /locus_tag="ACL8A1_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202357.1"
                     /translation="YYLNESGSMATGWFTVSGKWYYTYNSGDLLVNTTTPDGYRVNAN
                     GEWVG"
BASE COUNT         5561 a         3580 c         2939 g         5047 t
ORIGIN      
        1 gtgttttgag caacctgcgg ttagcttcct agtttgctct ttgattttca ttgagtataa
       61 tacaaaaatc cgatgaactt caccggactc ttttattttg aatttttgcc tgctttacgc
      121 ttttcagcga tttcggctgc ctttcgaggc aagacaattt ccgttatgta agccgtccca
      181 aaacgcagta cacctgcaat aggagcaaag acaactgcta gatagttata gaagaaatcg
      241 cctttgaagg cataagctag cgctccaatg atgaaaaata gaacgactgc ctgaatcact
      301 gctaataaaa ttactcgttt catgtgacct cctgactcta ttatagcatg agaatcatca
      361 aaaagccgac taaattattc aaagcgtgaa gagaaatact gtagactaga ccttttctgc
      421 taatgtaagc caaacccaaa ctaaaaccaa ggctaaaata gacaaaaaat tgttgcacat
      481 cacctggaaa atgaatcaag gcaaatagaa gactagatac cagaagaaaa atcagggttc
      541 gtttactatt gtcctgctta ggaaagagat agcgtgctaa catccctcta aaaacaatct
      601 cttccgtcaa aggagcaaaa ataaccacag caaagaatga gaaaagtggt tgagacaagg
      661 tcaagtctgt cgctatttgc tgatttactg aaggatcatc tggcaagaag aattgaacga
      721 ccagagataa gaaccaaacc aagacaggaa gccaaataaa tcggttaaag ccgctcttct
      781 caatatgaac aggagccttc tgataccatt tgtaaatgcc gtacacatat actacagcca
      841 aggccacata gagtagagta acagcatagg gtgaagcgcc taaagcaagc gacgcagtcg
      901 cgagcccctg aataaagcca tagataaata aaaaggatag aagggctaga agaatccagc
      961 caaggttttt aagtaatttc atagataact cctttatttg aaataacgtt ttaccatagg
     1021 taactgcatc acattgatat aaacatggat ggctcctaca agcaagaaag ctagtaactg
     1081 aatctctcct gtcaagaaag aaaggataat aagaaaaata tataaggctg gtaagacata
     1141 ttggtgtaat tggaataaaa ttcgaaaact ctgttccaaa ttagcctgac gctccccttc
     1201 atcataagaa tttatatagt tcaagacatc ctttggtgta gcgaaaaatt ccaaatcaaa
     1261 ctgacgaaca atcgcaatgg ttttaaaaag agatttttga gcgactaaga ataccacaaa
     1321 gagtaagaaa gaaaggaaaa atgtttgagg gtttgtatgc aatataatca cctcacttaa
     1381 tgaaataaaa atagccaatg gaatcgctac acctgtaata ttaaaagcaa tggttccaaa
     1441 ctcaagattc cgatacattt gcacataata ggtttcattc agatcgtcat ccatttcctc
     1501 ttgatacaag gaatgaaatt ttctgctttt ctttaagaaa ttgaaagtca aaaacatact
     1561 aatgaaacct atcagtaaac aaatagctga tatccatggc atcaaggctt ttacatctaa
     1621 aataatttcg tgggattcga cacgtgcctt aaacatccct acaaacatgc ccaagaaccc
     1681 ccccaagaca atagacatca aaaataacaa tctacgtttc tttttcatat tcattctcct
     1741 ttttcacttg ctagattttt ggatttcttt tcaatccatt caattactgg gatgagagca
     1801 aagtagaccc aaacaaattg gtcgctttga tagggattaa accagcttag gtccatccca
     1861 atcagtagaa atacgctgac taataaagct atgaccacta cataataaat cactttatac
     1921 ttgttcatca ctcgtcctcc tccaaacgaa ataccgattc gactgtttcg ttgaaaattt
     1981 gagatatttt cagggcaatg ataatggatg gggtgtactc atcccgttct agtaggctaa
     2041 tggtctgtct ggaaacccct gccagtttgg ctaggtcggt ttgattgaga ccatcgcgag
     2101 ctcgaagctc ttttagacga ttttttagtt gcacgttaca cacctactct ccgtcaaatt
     2161 caacggtttg gatatcttca atacgttgca acttgaattt ttcttttccc gtattatcta
     2221 cacgtcgtag ctttacccat tcctcatcaa catccacaac ttcccagtta tctggcccaa
     2281 tatacactcc cgttataatt ggtttctttc caatcatttc ttgcaataat ctcgacattt
     2341 ctgcgtttcc tttctctttt cgctcaagtt ttttgatttt attctctagt ttcttgattt
     2401 ttttagaatt attagaataa aagaaaataa taaatagtat aaatactagt acccacatca
     2461 tagctccttt cttcttttct cttaacttgc ttacattgta acacatcttt ttctttttga
     2521 caagtatagc tgtcaaaaag tttatgattt ttgtcatttt gcaaaagaaa aaggtcagga
     2581 gtaagttcct gaccacttta tctatcatta atactcttct aaaatctctt caaaccacgt
     2641 cagtttcatc tacaacctca aaaccatgtt ttgagcaacc tgcggctagc ttcctagttt
     2701 gctctttgat tttcattgag tataaaatcc tagtttttca aagatttctg agaagttttg
     2761 gctgattgtc tcaagtgaca cttgcacttc ttctcgggtt tggttgttct tgactgtcac
     2821 ttgtctgctt tcgacttcgc tctctcctag ggtgatgagg gtcttagccg caaagacatc
     2881 ggctgacttg aactgagctt tgagcttacg gttgaggtaa tcacgctctg ctttgaaacc
     2941 ttgttggcga agagcttgga ctagttctag ggctttgaca tttgctccat cacccaagac
     3001 tgcgatatag acatctaggg cattttcgat agggagggcc acgccttgtt tttcaaggat
     3061 gagaagcagg cgctctacac caagcccaaa accaaatcca gcagtttcag ggcctccaaa
     3121 gtaagcaacc aaaccatcgt agcgaccacc cgcacagact gtcaagtcat tgccctcaat
     3181 ctctgtgata aactcgaaaa tggtgtggtt gtagtagtcc agaccacgta ccatattggt
     3241 atcgatgatg taatctactc caagattttc caacatctga cgcacagcat caaaatgagt
     3301 ttggctttct tcatcaagaa agtccaagat agacggcgca ttctctactg ccaccttgtc
     3361 ttctttttcc ttagagtcca agacacgaag aggattttcc tccaagcgac gttggctatc
     3421 cttagacaag gtctccttga gcggtgtcaa atagtcaatc aaggcttggc ggtaggctgc
     3481 acggctctca ggatttccaa gagtgttgag gtgcaatttg acaccttgaa taccgatttc
     3541 cttcaaaaaa tgggctgcca tagcgattgt ttccacatcg gtagctggat tgctagagcc
     3601 aaaacactca acaccaatct ggtggaattg gcgcaagcgc cctgcctgtg gacgctcata
     3661 acggaacata ggtcccatat agtagaactt gcttggcttt tgcacttctg gggcgaaaag
     3721 tttattttcc acataggaac ggacaacggg tgcagttcct tctggacgga gggtaatatg
     3781 acggtcaccc ttgtcataaa aatcgtacat ttccttggtt acgatatccg ttgtatctcc
     3841 gacagagcga ctgataacct cgtaatgctc aaaaataggc gtgcgcactt ctgcgtagtt
     3901 gtagcgtttg aaaatttcac gggcaaagcc ctcaacgtac tgccacttgg cagattcagc
     3961 aggtaaaata tcctgcgttc cttttggttt ttgtaatttc atagggaatc ctctttaaac
     4021 ttaatagtct tattttacca taaatagagg gattaaaaca gtgagaaaaa aattaggatt
     4081 tagatatcat ttttgagatt aagaattgtt aaaaaaatag ctagcaagga aagaccaaca
     4141 aatagcatcc aagtcaactg catgttccat acggctacta gtgaaaaaca agctgttccc
     4201 acaggtatgg ataaggtaaa caatagacct aaaaaattac tagtaggagc tagaacctct
     4261 ggagctagat ttttcatgag catggcacta atctttggtt gaactttacc agacacatac
     4321 agagtaaaga agagaaatag caaaccaagc acgacttgat tgaataaatt agccaaacca
     4381 aaaggactgc ttcccaaaat aatcattgcc cgtaaggcta ctgatgatga ctgctactaa
     4441 aacacagaat tgattgataa atagtgcctc tgtataagaa aaattcaaga gagaatggct
     4501 caaaaagaag atattataaa ttccacccaa agcgccaccc aaggaattaa taagcaagac
     4561 agcaaagagc ataaaaccaa agtttttctg tccactttta agaaaaacga gacgtaaatt
     4621 tcggtaaatt gttaggaact ggtctttgat agaaagcttc tcatttttta gtttttcacc
     4681 atcagcagat gaaattgaca ggctcaattt gctttttcct aaaaaaagaa taaaggctga
     4741 tacgaggaaa aagcaagcat taattcccgc aacgagggaa aaattgttga ccgatagagc
     4801 taagagccag actccgaaag cttgaccacc aatagctgaa atataggtga tgaactgtga
     4861 aaaagaataa gcctccatca gatcatcttc agctactttt tccttaataa gaggcatacg
     4921 caggccacct gcaaaatcac tgatgatatc actaatgaca ttgatcaaac acaggctaga
     4981 aaaggcaaag agactagctt gctgaacaac tagggctgct aaaaaaaata gaaccgcctg
     5041 aaacaaaccg ctatagacca tccatttgac cttgtccctc gtgtaatctg cccgaatccc
     5101 tacaaaaact gtaaagaggg tcggaagaat catgacaata ttcgccatag caacagcaaa
     5161 agatgcttgt gacaaggtcg atgcatagac gataaagacc aggttgaaaa tcgaagcacc
     5221 aaaagcattg aagaagcgtg ataaagtcaa aattctataa gctggatttc taaacaatag
     5281 tttcatagat aatctccctc cttgttctaa gttctacatc aagaatagct ttagtatagc
     5341 acttatgaat ttgcgaaacg attcttttga atatatgcaa caaacaaaat gagcaaaaaa
     5401 acgatagaac ttttgtaatg acctgagaga gggcaacagt tctatcgctt caaatttttt
     5461 cttggtttgc agatattcaa gaatcgagag tttttctata gtattcggca gatttattac
     5521 agcccagcat ctcaaaaata cggacagcat cctccatctt tttctggcct tccttgactc
     5581 taccttgctt gctatcaagg agaccttctg cccacagata aacaattcgg aaataggtct
     5641 cattttcctt gtagaaatgc tcttcgataa cacgtttaaa ataataggca ttggtaaatt
     5701 cttcacactc aatactagct aaaaagccat tcaatagtat agtatgaaaa aggtttcgat
     5761 tgccagacat ttccattaga aaatcagatt tacgtaccat ttctcgtaca tatctagtaa
     5821 aaagagaaac agataaaaat ggagaactga ctgaaaataa attgagttca tagattcccc
     5881 agatctcggt agaaaacaaa taatcatgaa ggacttttcc ttcctctgct gttaactcta
     5941 ccctttcatc tatgctcttc atataagact tgataataat ggcatttaga atatgtttct
     6001 gtttgttgtg agaatgggca tgcttttgat actccctgcg atataagtcc tcaagaggtg
     6061 ctatattctt tggttccaag acatctgtaa tttcttttct caactcagaa tctgtatcat
     6121 actggaaacc tctcgccaga aagaggatct cctccacact ggcagatata ttttccagag
     6181 caaatagaaa cttttccacc gaaagctcac tctgacctgt ttcaaaacgg gacaacatag
     6241 acggcgaaaa ttgtcctccg gttgcttgtc tcagtgagat atttcttgac tctcgtaatt
     6301 gtctaaagac ttttccaatc tgctccatag acttcccctt gattccgtat tttcttcatt
     6361 ttatcatatt tttcagaaaa ttcatcaaaa acttgccaaa ttgtcagaat tatgagaaaa
     6421 tagaggatat ttatcacgtg gagggactgc tatgagagac gatatcaaaa tcaatgaccg
     6481 tgctttggcc ttgcaagacc aaattatcga aaaactagag aaagtttttg atacagatgt
     6541 ggaattggat gtttacaatc taggtctgat ttatgaaatc aatctggatg aaacggggct
     6601 ctgcaagatt gtcatgacct tcaccgatac tgcctgtgat tgcgccgaaa gcctgcctat
     6661 tgaaattgtg gcaggtctga aacaaatcga gggtatcaaa gatatcaagg ttgaagttac
     6721 ctggtcgcct gcttggaaaa tcacacgaat cagtcgctat ggccgtattg cccttggact
     6781 accacctcgt taagcaggcc aatcactttt aaagatgaaa aaagcaagcc gaaaatggct
     6841 tgctttttga tactcaatga aaatcaaagg gcaaactaga aaactagccg caagttgctc
     6901 aaaacactgt tttgaagtta tggatagaac tgacgaagtc agctcaaaat actgttttga
     6961 ggttgtggat agaactgacg aagtcagctc aaaacactgt tttgaggttg tagatagaac
     7021 tgacgaagtc agctcaaaac actgttttga ggttgcagat agaactgacg aagtcagctc
     7081 aaaacactgt tttgaggttg tagatagaac tgacgaagtc agtaaccata cctacggcaa
     7141 ggcgacgttg acgtgatttg aagagatttt cgagtatgag tttatttttt acctgacttg
     7201 tccatattcc agaagtctgt cacggctccg cgtgaagcag atgatacgat gtgggcatat
     7261 ttaccgagga caccacggct gtaaagtggt ggcaaggttg tttctgcctt gcgtttttca
     7321 agttcttctt cggatacggc catggaaatt tctttggtat cttggtcaac cgtaacgata
     7381 tcgccggtac ggagataggc aattggtcca ccatcctgag cttcaggagc gatatgtcca
     7441 acaaccagac cataagtacc accagagaaa cgtccgtccg tcaagagggc caccttatct
     7501 ccctgacctt taccaacaat cattgaagaa agtgacagca tctcaggcat accaggacca
     7561 cctttaggtc caacaaaacg aacaacgact acatcgccat caacgatttc atctgtcagg
     7621 acggcctgaa tcgcatcttc ttctgagtca aagaccttag ctggcccaac gtgacgacgc
     7681 actttaacac ctgatacctt ggcaactgca ccgtcaggag caaggttccc gttcaagatg
     7741 ataagcggac catccgcacg ttttggattt tcaagtggca tgataacttt ttggcctgga
     7801 gtcaagtctg caaagtcagc caagttttca gctacagtct taccagtaca tgtgatgcga
     7861 tctccgtgaa ggaaaccatt tgccaacaag tacttcataa ccgcagggac accaccgact
     7921 tcgtagaggt cttggaagac atactgacca gatggtttca agtcggccaa gtgaggcaca
     7981 cgttcttgaa tcgtattgaa gtcctcaagt gacaagtcaa catttgcggc atgggcaatg
     8041 gcgagcaagt gaagagtggc gtttgtagaa ccaccgagag ccatcgttac agtgatagca
     8101 tcttcaaagg cttcacgagt caagatatct gatggtttga gaccaagttc caacatctta
     8161 acaacagcac gtcctgctgc ttcgatatct tctttcttat cagctgattc agctgggtga
     8221 gaggatgacc ctggcaaact catccctaga acttcgatag cagttgccat ggtattagca
     8281 gtatacatac caccacaacc accagggcca gggcaggcat tacattcaag acgtttcacg
     8341 tcctcagctg tcatgtcacc gtggttccat tttccgatac cttcaaagac agaaaccaag
     8401 tcgatatctt taccatcaag atttcccggt gcaatagttc caccataggc gaaaatggct
     8461 gggatatcca tgttggcaat agcaatcata gatccaggca tgttcttgtc acagccaccg
     8521 atagcgacaa aggcatcgac attgtgacca ctcatagccg cctcgatgga gtccgcgatg
     8581 atgtcacgag atgttagaga gaaacgcata ccaggcgttc ccatagcgat cccgtctgct
     8641 acggtaatag ttccaaactg tacaggccaa gcgcctgcag atttgacacc ttctttagcc
     8701 agtttcccga aatcatgcaa gtgaatgtta catggtgtat tttccgccca agtcgaaatc
     8761 actcccacaa tcggtgtttc aaagtcctta tctgtcatac cagtcgcacg aagcatagca
     8821 cggttaggtg atttaaccat gctgtcatag atgctactgc ggtgacgttt atctaattca
     8881 gtcatcttat ccctcccatt tcagttttta ctattatagc acaattttcg catgaagaac
     8941 agaataaaat tcttgaattt tcagaaaatt ctatacacat gtgaaatatt taaaattaaa
     9001 aacaacaaag cggattagtg cactttctga tgaccagaat atgcttttta atccgctttc
     9061 tttaaataac gtaatgtaat ttttacagaa attctttcag aacagtgtat ttaacatcta
     9121 tcttgcatta taaaactcta gaaccttctc ttttatattc gattcactca aaccatactc
     9181 attaagaaga taatccattt tccctacttg accgaatctt tcttgaacac ccatccgatg
     9241 aatttttgtt attccatcat cagagaataa ttcacataaa gcactgccaa ttccacctat
     9301 ctgattgtgg ttttctacag taaatatagt ttttccactt aacattgttt ttatctgttc
     9361 tggtatcggt ttgattctaa ataaatctat cacacctact gaataaccta atttagacag
     9421 ttcatctgca actcgaatac ttggagcaac cattatgcca gaagcaacga ttacaagatc
     9481 ttcaccatgt cttaactcaa tgtagccttt agaaaaatcc tctccacctt gatacacagg
     9541 aactggagct tttctaattg ttcgaatata ttttagtcct tttaagtcta atgtctggtt
     9601 caatatttca cgaaattgga tatcatcagt tgcttcaaaa atgatcgatt taggaattaa
     9661 acgtaacaat ccaatttctt caaatggcat atgtgtccca ccattcattt ctgcagttac
     9721 tcctgcatct gatccaatca cagtggcatc caattgtgcg tatccaagag aaataaataa
     9781 ttgatcaaat actcttcgtg aagaaaaagg accaaatgta tgaagataag gtctaaaccc
     9841 ctgaatagac aagcctgctg caaggccgac catttctgct tccataatcc caacattcac
     9901 ataacggtct ccaaagtcct tttcaagatt atgagtagcc atcgaacttg acagatcggc
     9961 ttctaagact actatatcag aatcactttg attagcctct aaaaggaagt ctctatatac
    10021 atgccgtaat tctttcgtac ttctcatcat tctgtttcct ccaattcctg acttaatctt
    10081 tctacaactg aagttaacat ttgtctctcc tctacagtag ggcgaagatg atgattggat
    10141 ttcatttctt ccagctcttg aaccccttga cctttaatag tatctaatac aatgcactta
    10201 ggtgatgaat tatttgactg ttttaattgg acaatccctt cataaatttc tctaatatct
    10261 gaacccttga ccctaatgga ttcaaatcca aatgctgaaa atttttctac gaaatcacct
    10321 ggattacaaa tatcctttgt aaaaccatct aattgttttt tgttatcatc aacaaataca
    10381 attaagttgg ataactgttg atgagaagca aactgtatag cctcccaaca ttgtccctca
    10441 tttaactcac catctccaac aatagcataa gtataaaagg gactctttct tattctctga
    10501 ccatatgcaa gtccagttgc aacactaatt ccttgtccta aagagcccgt tgtcatatct
    10561 atgcctggcg ttagatttct atcaggatga gacggtaatt tggttccatt tgtatttaaa
    10621 gaatataaga attctttgtc aaagaaacca ttcaaataga gtgtactgta tagagctggt
    10681 cctccgtgac cttttgataa tatgaaataa tctctatctc gtgctgcaaa tatttctgga
    10741 gtcattggca ttatttcacc ataaagcact gctaaaactt ctacgataga cagacttcct
    10801 ccgtaatgtc cgaatccaag atgattcaat gttctaagag tatttaatcg gatgttagtc
    10861 gcaaattttc ttaacccatc ttctctattt ttacttaaaa tcatccctta ttcctccgtt
    10921 gcagatggct ttttaataaa ggatactcca aacataactg ctagaataag aacaagacca
    10981 atcacaatgc ctgcttgtga gccaaattga tttaatattc ctaaaataat tcctgataga
    11041 ccaaaatctg catctgagaa agttgatcct tggaaaccaa gtcctcccaa aactggcatt
    11101 aaaaagactg gaagaaaact gattaaaata ccttgtaaaa atgctccaat agtggctcca
    11161 cgaacaccac cagatgcatt cccaatgaca cctgcagtcg ctccacagaa gaaatgaggc
    11221 acaacacctg gtaagataac aaccgttcct gaagcaatca taattgccat acttactaaa
    11281 ccaccaacaa aactagagat aaatccaatt agaactgcat tgggtgcata agtataaaca
    11341 atcggacaat ccaaagcagg ttttgaatta ggtacaagac gctctgaaat acctttaaag
    11401 gctggaacaa tttcgcccaa aataaggcga acacctgcta aaataacaaa tacccctgct
    11461 gcaaattgac ctgctaattg taaagcataa actagaccac ttgtaccact actgatttct
    11521 ttttctatat attctgaccc tgcaaagata gctacaataa tgtaaataac tgccatagat
    11581 aaagtgatac taacagtact atcacgtaaa aaagctaaac tctttggaaa tttaatgtcc
    11641 tctgttgatt ttgatttgtc accgataagg ctaccagtaa aaccactcaa ccaatatccc
    11701 aaagaactga aatgacctaa agccaccttg tcatttccag ttaattgaac catatatttt
    11761 tgcacaaatg ctggggaaat actcataata ataccgagtg ctaatcctcc tagtaagatg
    11821 agaggcaagc tagtaaagcc agcaactgat aaaatgaccg caatcataca tgccatatat
    11881 agagtgtggt gccctgttaa aaagatatat ttaaatcgag taaaacgagc gattaagata
    11941 ttgaacacca tgcctgcaaa cataatcatt gcagtagctg agccatatgt tgttaaagct
    12001 acagctacaa ttgcttcatt attcggcaca acgccagata aatgaaaagc atgctcaaac
    12061 atggtaccaa atggatttaa agaattttgt acaattcctg caccaccaga tacaactaag
    12121 aaaccaacaa aggtcttaat tccaccttta ataatatcag gtaatttctt cttctgaaga
    12181 actaatccta agattgcaat taaagctact aaaatagctg gtgtactaac aatatccaat
    12241 atgaacttca tcatgacgct agcctcctat ataagtcctt tttcttcaca aattttagta
    12301 attaattctc gtagttcgtc catatcaata atactattta agatacgaac atctccaaga
    12361 tgactagctg aatcagctag atcacgacca acaatccaaa tatcagctgc atttggatct
    12421 gctccaccta aatcataatg ttcaacttct acatccgaaa cattcaaatc actcaataca
    12481 gattcaatat tcatctgtac cataaaactt gaacctaatc ctgaaccaca agctgtacca
    12541 atttttaaca ttatctaatc ctcctgttta attatcattt taatatcatc atagtttttt
    12601 gatgatatta aagtttgaac atgattttta tctcttaaaa ttgttgttaa atgtgacaaa
    12661 gcctttaaat gactctcatt atcaatggct gcaatacaaa tcaacaatct tacctcttgt
    12721 tctggattat ccaataaata aatcggttct tccaaaacta acattgacat tcctatttca
    12781 ttcacacctt catctggccg agcgtgagga attgctactc ccttccctaa attaataaaa
    12841 ggtccaaact cttctacttt ttgaatcatt gcctcagggt agttctccgt tatcttatct
    12901 tgatccaaaa gcggtttagc tgctaaacga atcgcctcct tccatcctaa tttttgcgaa
    12961 ctaacctgat aggtttcttt ggtaataagt tgttctagca ctggtacaat ttcctttcta
    13021 tcattttttt gataaagata attctttaac gccaatctta attccaattc ttgtgtaata
    13081 attccatatc ttttgacaat attcaggatt tgttcaatct caaaatctcc atactctaaa
    13141 ttcggaaaat cttttaacac tagttctact agttgtattg cttgctcttc agtcatcata
    13201 accgaaacta gataatttgg cttttctgtc tccaccttta tggtagaaaa aaccatatca
    13261 tagtcactac tagctttcac ctgtaaatca tcaatctttg aggttcctat aaactcaatt
    13321 tgaggaaata atgctaatag attctctttt aacatcaatg aagaactaac accattagga
    13381 caaatgattg ctgctttata ccatttttga ggcaaagtat ctgctttctt taaataacct
    13441 ccgaaatgga taacaaaata tgctgtttca ctatcaggta tgggattgtc aatagcgtcc
    13501 atcaagggca tcaaagaatc tttgactaat tcaaataaat caggataatg ttctttaaca
    13561 tgcaatacat attcatttga actaggtagg ccgaacttta atctatagta agccggtata
    13621 aggtggcgac gaagattttc tctcaatcct tccctttgtt taaaatgtaa caaagaaata
    13681 tcttccattc tacttataat agcctctgtt aattgattaa agtaaaccgg agcaacatct
    13741 acttcacctt caaagcaact tgataataaa actgtgatat agcgataatc atcctcagaa
    13801 aacaccgtat ctataattcc caaatcaacc actgtatcca ataaaatagt cgttatatct
    13861 tgaataacag gagatactaa tgtctctgaa agacatactc tttcaacatc cctttgatac
    13921 ctacacagaa tgaatactaa accgaaaagg taaactttta attgattaac aataggtact
    13981 agctgtagct tctcataata atctttaact acctgatcaa tcaaatcata agttaatgaa
    14041 tacccccaac tggataaaac ataatccaaa ccccaaatcc ctatggagga ttccagcaac
    14101 tcactaacca tttgaaaagc taaacggtgc ttattccact ctgaaccgtg taaagtataa
    14161 ccttttgctc tactgtaccc tagctccaaa tcattatcta acataatctt tcttaatgat
    14221 tgaatatcag ataaggttgt attcttactt actttcaaaa agtcttggta atgattattc
    14281 gatataaaat ctaatcggca aaaagtgtaa agatagatta aagctaagcg agtcgacttt
    14341 ggtaaaacca attcatccga cttaataata tctgtcaaag actgcttcgt acgatttgat
    14401 aaactatagc gaccttgttt tttatccagc actatccctt tattagctag ataaggcact
    14461 aaataatcta ttccttcttt gacttccttt ataggtaagc tcaccttaac agataattca
    14521 tataatgata gctcacaatg atccatcaaa gtcatcaaaa taactagtgc tctataatca
    14581 aacatttctc ttcctttcta actacaatta tagtgtaaaa attataagaa taaaagagta
    14641 tttgagcaca atataatctc accttttaaa agaaattatt gggctgaaag tttttagtga
    14701 ttattttcaa ctttctattg acttttaaaa acaaagtgat atcatttaga tatcaaataa
    14761 tgggaggtca ttcttatgac tgaaaaacta atcaattcaa aaccaaatgg tgtattcgca
    14821 ttgattctca ttgagttgac aatcatactt ggtatcttta tatttataat gggtgttggt
    14881 tcggaaaaca tttttggaat tattatcgga cctttactaa tcgtaattgc aggtctagct
    14941 catgctggtt taaaagttgt caaacctcag gaagctctgg ttctgacact atttggtaat
    15001 tatacaggta ccatcaaaga acctggtttt tactttgtca atcccttcag cgtagcagtc
    15061 aaccctgcaa accacactcg acttggacaa agtggtgatg ttagcacaaa atctcctttt
    15121 ttaggagcta aatcatcaaa tgacaatgat gtaaatcttg aaattggcaa gaaacacatt
    15181 tccctcaaag tcatgacctt gagcaattct cgtcaaaaaa tcaatgattg cttaggaaac
    15241 cctgtagaaa tcggtatcgc ggtaacttgg agagttgtcg ataccgctaa ggcagtcttc
    15301 aacgttgata actacaaaga atatctttca ttgcaatgtg atagcgccct ccgtaatatt
    15361 gtccgcatct atccttacga tgtgtctcct aatgtggata ctacgggtga cgggcaagca
    15421 gatgaaggta gtctccgtgg ctctagcgaa attgttgcta accgtattcg tgaagaaatc
    15481 caaagtcgtg ttgaggatgc tggcttggaa atccttgaag cacgtatcac ttacctagct
    15541 tatgctccag aaattgctgc cgttatgctt caacgccaac aagcatctgc cattattgat
    15601 gcacggaaga tgattgtaga tggtgctgta ggaatggttg aaatggcact agaacggctc
    15661 aatgaagggg aattggtaga acttgacgaa gaacgaaaag ctgccatggt ttcaaatctc
    15721 cttgtcgttc tttgtggcaa tcatgatgca caaccaattg tcaacacagg aagtctttac
    15781 taaagatggc tgaagctaaa aaaaagcaga tcccccttcg actctcaaca aagttatacg
    15841 ctgcactcgc atcatgggca gaagacgact ttcgttcagt caatgggcaa atcgaatatc
    15901 tcctcacaga gtgtgtcaag caacggaaaa aagacggaaa atacgtatca gaaaccattg
    15961 acgaaccatt tgagattgat atttaacatc ttctcctgtc tgacaagtcg gacaggagaa
    16021 ttttttcatc tttttttgtc aagtaacttt actttacaaa aaaaatgtgt tatcctagta
    16081 tggttgatga aaatcagtag attgaatcga atataaatac ctaaaggaga aatcaaaatg
    16141 gcagtacctg cacgtcgcac ttcaaaagcg aagaaaaaca aacgtcgtac acactacaaa
    16201 gtaacagctc catctgtaaa ctttgacgaa actactggag attactcacg ttctcaccgt
    16261 gtatcactta aaggatacta caaaggacgt aaaatcgcta aagctgcatc agctgaataa
    16321 tagaagggag ataccatgcg cgtaaatatt acacttgaac acaaagaatc tggtgaacgc
    16381 ttgtacctta cttctaaaaa caaacgtaac actccagacc gccttcaatt gaagaaatac
    16441 tcaccaaaac ttcgcaaaca cgttgtgttt acagaagtga agtaagcatt caatacgaat
    16501 caatacagaa gaaaaacgct tgatcagcgt tttttctttt tgttaatttc actatattgc
    16561 aagggaaatc attcgaatga ctaccttttt cactaccttt tttgaaataa gaatacggat
    16621 tcggacgatt aaaagtaaga gaaactcaaa tgtaagttgg ggttcctttt ttgtatattt
    16681 ttcaaataac gcctctacac tatttgtagc aaattcacca actacagttg tatcttagtt
    16741 aaaataagtt agaatatgta agtgagtacc agatatacca agacatcgtc accatctaag
    16801 gtatattcaa aatacaaaag ttgaccaact agatttctga atatccttat atatccattc
    16861 ttaaaattgg tttaaatagc gtagtctttt aaactagttt tgagaatcca aaaaatcttc
    16921 ctacatatgt aagaagattt tttagttcag aatgattaga tttagctaat ggatacctat
    16981 cctacccact caccgttagc attgactcga tagccatcgg gtgtagtcgt gtttactaat
    17041 aaatctcctg aattataggt atagtaccat ttaccagaaa ctgtaaacca accagtagcc
    17101 attgaacctg attcatttaa gtaatac
//
LOCUS       DBERMR010000044        16569 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.044, whole genome shotgun sequence.
ACCESSION   DBERMR010000044 DBERMR010000000
VERSION     DBERMR010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 16569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16569
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.044"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(124..840)
                     /locus_tag="ACL8A1_001616"
     CDS             complement(124..840)
                     /locus_tag="ACL8A1_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGS1202358.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
     gene            1188..2975
                     /locus_tag="ACL8A1_001617"
     CDS             1188..2975
                     /locus_tag="ACL8A1_001617"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HGS1202359.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFDGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVSRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            2972..3448
                     /locus_tag="ACL8A1_001618"
     CDS             2972..3448
                     /locus_tag="ACL8A1_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS1202360.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            3476..4381
                     /locus_tag="ACL8A1_001619"
     CDS             3476..4381
                     /locus_tag="ACL8A1_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HGS1202361.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            4368..5183
                     /locus_tag="ACL8A1_001620"
     CDS             4368..5183
                     /locus_tag="ACL8A1_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGS1202362.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            5190..5594
                     /locus_tag="ACL8A1_001621"
     CDS             5190..5594
                     /locus_tag="ACL8A1_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGS1202363.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNMVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKIRAAESIVKVNDLLASFDDDEDE"
     gene            5888..7054
                     /locus_tag="ACL8A1_001622"
     CDS             5888..7054
                     /locus_tag="ACL8A1_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HGS1202364.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            7170..8207
                     /locus_tag="ACL8A1_001623"
     CDS             7170..8207
                     /locus_tag="ACL8A1_001623"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HGS1202365.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHIYNGTLLKDIFAEEDEQIQLASGLDHPFALPVGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            8415..9269
                     /locus_tag="ACL8A1_001624"
     CDS             8415..9269
                     /locus_tag="ACL8A1_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGS1202366.1"
                     /translation="MTYEYKSHIYLAETALNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTRIRLAR"
     gene            9419..9676
                     /locus_tag="ACL8A1_001625"
     CDS             9419..9676
                     /locus_tag="ACL8A1_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HGS1202367.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADIVTQLTAVRSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
     gene            complement(9718..10080)
                     /locus_tag="ACL8A1_001626"
     CDS             complement(9718..10080)
                     /locus_tag="ACL8A1_001626"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202368.1"
                     /translation="MKIKVQTRKLAAGCSKHCFKVVDGTDEVSSKHCFEVVDRTDEVS
                     NHIYGKVKLTWFEEIFEEYKSLHNKTHITKVLMNCTPKVRQKKSNFLGSVHSTPDTMR
                     FFNFKDFLQLLLTHIKLF"
     gene            complement(10134..10430)
                     /locus_tag="ACL8A1_001627"
     CDS             complement(10134..10430)
                     /locus_tag="ACL8A1_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HGS1202369.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPDAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLSSQGLTVTNVEGGMNAWPGQVE"
     gene            complement(10420..10575)
                     /locus_tag="ACL8A1_001628"
                     /pseudo
     CDS             complement(10420..10575)
                     /locus_tag="ACL8A1_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691454.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
     gene            10652..11416
                     /locus_tag="ACL8A1_001629"
     CDS             10652..11416
                     /locus_tag="ACL8A1_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HGS1202370.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGADCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            11981..13360
                     /locus_tag="ACL8A1_001630"
     CDS             11981..13360
                     /locus_tag="ACL8A1_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HGS1202371.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            13374..14039
                     /locus_tag="ACL8A1_001631"
     CDS             13374..14039
                     /locus_tag="ACL8A1_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HGS1202372.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            14174..14332
                     /locus_tag="ACL8A1_001632"
     CDS             14174..14332
                     /locus_tag="ACL8A1_001632"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202373.1"
                     /translation="MKIKDQTRKLAAGCSKHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     NIYTARRR"
     gene            14394..14933
                     /locus_tag="ACL8A1_001633"
     CDS             14394..14933
                     /locus_tag="ACL8A1_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGS1202374.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKGHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQEFESKGQEVDVFVTNFVYEKEGQSCKKSMSYDSVLPVRQIFGWDQVGNFSKGQ
                     YIMMHSLIYRTDLLRASQF"
     gene            15000..15410
                     /locus_tag="ACL8A1_001634"
     CDS             15000..15410
                     /locus_tag="ACL8A1_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HGS1202375.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLVDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSGTAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            15526..>16569
                     /gene="pavB"
                     /locus_tag="ACL8A1_001635"
     CDS             15526..>16569
                     /gene="pavB"
                     /locus_tag="ACL8A1_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HGS1202376.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVEKAVKDNVKDSIDVPAAYLEKAKGEGPFTAGVN
                     HVIPYELFAGDGML"
BASE COUNT         4652 a         3092 c         3752 g         5073 t
ORIGIN      
        1 ggttctattt ctataaaata ttccacaaat tatagaatct tccaaaaata ggtaagcgct
       61 acctttttgg tgtggtataa taagcataga aaaagcccga gcgattaggc tcaggctttc
      121 ttcttagtga tcacggtcac atgagatgaa tttaatcttg tagtaatcag atcgtttgta
      181 agtttcactg tattctaaaa cttggccagt tgattcgagt ttggtgattt tagtttgtag
      241 gacagtaggg aattgttcat cgactccgag gactgaagcc gcatgttctg gagttggaaa
      301 gactatttcg ttgatttctt caaagtgttc atcattcatg tgaatgtggt agtctaactt
      361 gaaacgatta tagatagaac tatagtattc aaggtttgga taatttgcgt tgatatattg
      421 ttctgggatg taggatgtat ggtagatata aacgacaccg tttgattcgc ggatacgttc
      481 aatcttgtag tagaattgat cgccgcgtag acccaatttt tccaagtaaa caagcttgtt
      541 tccgcgttca attgaaagaa cagttacctt atcatcttta gcattgaaga gttcaatatc
      601 tgaaaactct acaagcttgt gtttgcgtgc acgtgaaacg aaggttcctt ttccttgttg
      661 gcggacaata tagccatctt tggcaaggtc gtttaaggcg cgaacaactg tgatagagct
      721 gacatcgtac attgaaatga gttctgcttc agtgtaaaat ttatctccac tgctaaactg
      781 cccagagatg attttatttt ttaattcgtc ttttatgtat tgatatttgg gaattgccat
      841 atttttacct ctattttcct tctccataga aaattttacc ataaaagcga aaaaaatagt
      901 agtcttttga ataaaaaatt agaataatag tttgaaaaat taagcgatat agggattgct
      961 cgtattttat aaatgtattc tatacaaaat tattttaagt cctatatttc gaatttaaag
     1021 agcgtttgaa atgggtctaa aaatgtttga aactaattga atgtgtgtat aatagtaaaa
     1081 atgcagtaaa tatcccgaca atttctattg acaattcaaa cagattggtt tataattaat
     1141 ataacaacaa atgaaagcgc aaactttcgt ggtcggaagg tagttttatg acacgatttg
     1201 agatacgaga tgatttctat ctcgatggaa aatcatttaa gattttatct ggtgccattc
     1261 attattttag ggttcctcca gaggattggt atcattcgct ctataacttg aaggctcttg
     1321 gttttaatac ggtagagact tatgttgctt ggaatttaca cgagcctcgt gaaggtgaat
     1381 ttcattttga cggtgatctg gatttagaga aatttctcca aatagcgcag gatttgggtc
     1441 tctacgcaat tgtgcgtccg tctccattta tctgtgcgga atgggaattc ggtggcttac
     1501 cagcttggct cttgaccaag aacatgcgaa ttcgctcatc cgacccagca tatatcgagg
     1561 cagttggtcg ctactatgat cagttattgc caagactggt gtctcgtttg ttggacaatg
     1621 gtggcaatat tctcatgatg caggttgaaa atgagtatgg ttcttacgga gaagataagg
     1681 cttacctgag agcgattcga cagctaatgg aagagtgtgg cgtaacctgt cccctcttta
     1741 catcagatgg tccatggcga gctactctga aagctggaac cttaattgaa gaggacctct
     1801 ttgtaacagg aaactttggt tctaaggcac cttacaactt ttcgcagatg caggaattct
     1861 ttgatgaaca tggtaagaaa tggccactca tgtgtatgga gttctgggat ggttggttca
     1921 atcgctggaa agagccgatt atcacacggg atcctaagga attggcagat gcagttcgag
     1981 aggttttgga acaaggctct atcaatcttt acatgttcca cggtggtaca aactttggtt
     2041 tcatgaatgg ttgctcagct cgaggaactt tggacctgcc acaagttaca tcttatgatt
     2101 acgatgccct tctggatgaa gaaggaaatc caactgctaa atatcttgca gtcaagaaga
     2161 tgatggcaac acatttttca gagtatccgc agttggaacc actctacaaa gagagtatgg
     2221 agttggatgc tattccacta gttgaaaaag tttctttgtt tgaaacctta gatagcttgt
     2281 caagtcctgt agaaagtctc tatcctcaaa agatggagga gctgggacaa agttatggct
     2341 acctacttta tcgaacagaa acaaactggg atgcagaaga agaaagactt cgtatcattg
     2401 atggtcgaga tagggcccag ctgtatgtcg atggtcagtg ggttaaaact caatatcaga
     2461 cagagattgg ggaagatatt ttttatcaag gtaaaaagaa agggctatct aggttagata
     2521 tcttgataga aaatatgggg cgtgtcaact atgggcataa gttcttagcg gatacgcaac
     2581 gtaagggaat tcggacaggg gtctgtaagg atctgcattt cttactaaac tggaaacact
     2641 atccactccc actagacaat cctgagaaaa ttgatttttc aaaaggatgg actcaaggac
     2701 aaccagcctt ttacgcttat gactttacag tcgaagagcc aaaagatact tacctagact
     2761 tgtctgagtt tggtaaggga gttgcctttg tcaatgggca gaatctagga cgtttttgga
     2821 acgttggccc aactctctca ctttatatcc ctcatagcta tctcaaggaa ggtgccaacc
     2881 gtatcattat ctttgaaaca gaaggtcaat ataaagaaga gattcattta actcgtaaac
     2941 ctacactaaa acatataaag ggggaaaact tatgacaatt gtaggatgcc gtattgatgg
     3001 acgtttgatc cacggacaag tagccaatct ttgggctgga aaactaaatg tttcacgcat
     3061 tatggttgta gacgacgaag ttgtcaacaa cgatattgaa aagagtggtt tgaaacttgc
     3121 gacaccacca ggtgtgaaat tgagtatttt gccagttgag aaagctgcag ccaatattct
     3181 tgctggtaaa tacgatagcc aacgtctctt tatcgtggct cgtaaaccag accgcttcct
     3241 tggtttggta gaagcaggtg taccacttga aacccttaat gttgggaata tgtctcaaac
     3301 accagaaact cgttctatta cacgttctat caacgtagta gacaaggatg tggaagactt
     3361 ccacaaactg gcagaaaaag gtgttaaact tactgctcag atggttccaa atgatccaat
     3421 ttcagacttt ttgagcttat taaaatagga aaaaaatttt taggaggtca ttgttatgat
     3481 acaatggtgg caaattttac ttctcacttt gtactcagct tatcaaatct gtgatgagtt
     3541 gacgatcgtt tcatctgcag gttcccctgt atttgctggt ttcattactg gtttaatcat
     3601 gggagatgtg actactggtt tacttatcgg tggtaacttg caactgttcg ttcttggggt
     3661 tggtaccttc ggtggtgctt ctcgtatcga cgcaacttct ggtgcggttc ttgcgacagc
     3721 cttctctgtt tcacaaggaa ttgatgcacc gcttgccatt actacaatcg ctgtaccagt
     3781 agcagctctc ttgacttact tcgacgttct tggtcgtatg actactacct tcttcgctca
     3841 ccgtgtggat gctgcaatcg aacgctttga ctataaaggt attgaacgca actacttgct
     3901 tggtgcgatt ccgtgggctc tatctcgtgc ccttccagtc ttctttgccc ttgcttttgg
     3961 tggtgccttt gtacaatcag tagtagactt cgttgaagcc tacaaatggg ttgcagatgg
     4021 cttgacactt gcaggacgta tgcttccagg tcttggattt gcaatcttgc ttcgttacct
     4081 tccagttaaa cgtaaccttc actaccttgc tatgggattt ggtttgacag ctatgttgac
     4141 tgttctttac tcatatgtaa caggtcttgg tggcgctgtt gctggtatcg taggtactct
     4201 tcctgctgaa gttgctgaaa aaattggttt cgtgaacaac ttcaaaggtt tgtctatgat
     4261 tggtatttct atcgtaggta ttttccttgc agtgcttcac ttcaaaaata gccaaaaagt
     4321 agctgtagca gcaccttcta caccatcaga aagtggggaa atagaagatg acgaattcta
     4381 attacaaact tacaaaagaa gattttaatc aaatcaacaa acgtagcttg tttactttcc
     4441 aattaggttg gaactacgaa cgtatgcaag cttctggtta cctttacatg atcttgcctc
     4501 agttgcgtaa aatgtatggt gatggaactc ctgaattgaa agaaatgatg aaagttcata
     4561 ctcaattctt caatacttcg ccattcttcc ataccattat cgctggtttt gaccttgcca
     4621 tggaagaaaa agatggtgta ggttcaaaag acgccgttaa cggtatcaag acaggtttga
     4681 tgggaccatt cgctcctctt ggggatacaa tctttggttc acttgtacct gctatcatgg
     4741 ggtcagtcgc agcaactatg gctatcgctg gccaaccttg ggggatcttc ctttggattg
     4801 cagttgcagt agcgtatgac atcttccgtt ggaaacagtt ggaatttgct tacaaagaag
     4861 gggttaacct tatcaacaac atgcaaagta ccttgacagc tttgattgac gctgcatctg
     4921 tacttggtgt cttcatgatg ggtgctcttg tagcaacagt gattaacttt gaaatttctt
     4981 acaagttgcc aatcggtgaa aagatgattg atttccaaga catcttgaac caaatcttcc
     5041 cacgtttgct tccagcaatc tttactgcct ttatcttctg gttgcttggt aagaaaggta
     5101 tgaactctac taaagctatc ggtattatta tcgtacttgc tttggctctt tctgcccttg
     5161 gtcactttgc acttggaatg taattcctta tgactaaatc attaattttg gtgagccatg
     5221 gtcgcttctg tgaggagctt agaggtagca cagaaatgat tatgggccca caagacaaca
     5281 tttacacagt agctcttctt ccagaagatg gcccagaaga atttactgct aaatttgaag
     5341 ctgttattga agggttggat gatttcctag tctttgcgga tcttctcggt gggacacctt
     5401 gtaatatggt gagtcgcttg atcatggaag gtcgtgatat tgacctttac gcagggatga
     5461 atcttccaat ggtgattgaa tttatcaatg cgagccttac aggcgcagat gcggactaca
     5521 agatccgtgc tgcagaaagc attgtgaaag ttaatgacct gttagcgagc ttcgatgatg
     5581 acgaagatga ataatactct tcgaaaaact cttcaaacta cgtcaacgtc gccttgccgt
     5641 aggtatatgt tactgacttc gtcagtctta tccggcaacc tcaaaacggt gttttgagct
     5701 gacttcgtca gttctatctg caacctcaaa gcagtgcttt gagcagcctg cggctagttt
     5761 cctattttag ttggaactcg attcaattca tgtgacaacg tgaaaatcgt tagagcattt
     5821 tatatagaat atacatggga atgtagctta ctctcattcc catatttaat agaaaaagag
     5881 gaactcaatg ctacattata caaaagaaga cttgctcgaa ttgggtgcag aaatcactac
     5941 gcgtgaaatc taccaacagc ctgatgtatg gagagaagct tttgaatttt atcaagcaaa
     6001 acgtgaagaa attgcagcct tcctacaaga aatcgctgat aaacatgact atattaaggt
     6061 tatcttgaca ggtgctggga cttctgctta tgtgggagat accttgctac cttattttaa
     6121 ggaagtctat gacgaacgca aatggaattt caatgctatt gcgacaacag atatcgttgc
     6181 caatccagca acctatttga aaaaagatgt ggcaactgtc cttgtgtctt ttgctcgtag
     6241 tgggaactcg cctgaaagtg tggcgactgt tgatttggcc aaatccttgg tggatgagct
     6301 ttatcaagtg acgattactt gtgcagcaga tggtaaattg gctcttcaag ctcacggtga
     6361 tgaccgtaat ctcttgctct tgcaaccagc tgtctctaat gatgctggat ttgccatgac
     6421 ttctagcttt acgtctatga tgttgacagc tctcttggtc tttgatccta cagaatttgc
     6481 tgttaagtct gaacgttttg aagttgtatc tagtcttgcc cgtaaagttt tagacaaggc
     6541 agaagatgtc aaagagctcg ttgatttaga ctttaaccgt gtcatctatc taggcgctgg
     6601 tcctttcttt ggacttgctc atgaagctca gctcaagatt ttggaattaa ctgctggtca
     6661 agttgcgacc atgtatgaaa gcccagttgg cttccgtcac ggtccaaaat ctcttatcaa
     6721 cgacaataca gttgttttgg tctttggtac aacgacagac tacactcgta agtacgactt
     6781 ggacttggtt cgtgaagttg ctggtgacca gattgctcgt cgtgttgtgc ttttgagtga
     6841 tcaagctttt ggtcttgaaa atgtcaaaga agtggccctt ggttgtggcg gtgtcttgaa
     6901 tgatatttac cgtgtcttcc cttacatcgt ttatgcccaa ctctttgccc tattgacttc
     6961 actcaaggta gaaaataaac cagatacacc gtctcctaca ggtacagtaa accgtgtagt
     7021 acaaggtgtc ataattcacg aatatcaaaa gtaagacagt gtttatgaat tcttgataag
     7081 aggatttgta aattatcaga taaaccatag attgtcagta tgctttctat ggtttgtttg
     7141 cttgagagaa atagtaaaag gagaacagaa tgaaagcata cacagagcgt gtatttggaa
     7201 atgttgaggg cgaggatgtc ttggcctatc gatttgagac agacggtggc taccaacttg
     7261 aggttatgac ttatggcgcg accatcttgc gctatgtcgc acctgacaag gctggaaatt
     7321 ttgccaatgt tatcttggga tttgatgact ttgatagtta tgtaggcaat agtcccaagc
     7381 atggagcaag tgtaggtcct gtagcgggtc gtattgcagg tgcgaccttt gagctcaatg
     7441 gtaagaccta tgaccttgag gttaataatg ctagcaactg taatcacagt ggttcaactg
     7501 gttgggattc cagcttgttt gaagttgaag aagtaagcga tcatggcttg actctctaca
     7561 cagagcgtac agatgggaca ggagggttcc ctggaaatct caagatttgg atcagttatc
     7621 acttggaaga aactggtgcc tatgaaatca gctacaaggt aacgaccgat caggatacgc
     7681 tggtcaatcc aaccaaccac agctatttca acttgtctgg tgatttcacg cagacgattg
     7741 accgtcatgt cttccaacta aacacagagg gcatttactc aatcgctcct gacggtgttc
     7801 ctgccaaaac tccagaagcc aaccgtgatg tggtcaaaca catctacaat ggtaccttgt
     7861 tgaaggatat ctttgcagaa gaagatgagc aaatccagct ggcatcaggt ttggatcacc
     7921 catttgccct tcctgtaggc catgacaatg ctggattcct ttatgaccaa aattcaggtc
     7981 gcttcctgct tttcaagaca gaagctcctt gctttgtggt ctacacagca aactttgtgg
     8041 atgaaagtgt catcatagga ggtcagccaa tgctacagca caatgggatt gctcttgaag
     8101 cacaagcttt accagatgcc attcacagtg accttaaagg ccaagtcatt cttaaagctg
     8161 gtcaaacctt caccagtaag acacgttatg aacttgttgt gaaataaaaa aagctggtca
     8221 aaaagtcttt aaaattaaaa taatgcatat tgtcaggtga tggaaagttt gataatatgc
     8281 gttttattat aaaatgattt actttatctt atcctgaaat ggtgttattt ttgagttatc
     8341 tggcttgact ttcttgatga aaattatata atagttgtaa taataataat tacaacgaga
     8401 aaagaggacc atttatgacc tatgaataca agagtcacat ttatttggca gagacagctt
     8461 taaatgtaaa ggatttggcc agtcaaacag ccttttatca gcaagttatt ggtttagaaa
     8521 tcttatctca aactgagaca gagtccattc taggccttgg tggaaaagtc ttggtacagc
     8581 tgattcaagc acaagagagc ggagaagtga gggaacatta tggtctttac catttggcca
     8641 ttctcttgcc cacacgcaag gctttggctg atgtcttgaa acacctgaca gatttacaga
     8701 ttcctcttgt cggcggtgca gaccatggtt acagtgaagc tatttacttg gaagatttgg
     8761 agggaaatgg cattgaactc tatcgagata agccagtttc cacatgggat attcgagaag
     8821 atggacgcat tatcggagtg acagaggctc ttgcggcgca ggacatttat gagttggggg
     8881 aaagagtaga gccctttatc ctggcagaag gtacgagaat ggggcatatt catctttccg
     8941 tcaaggatag tcgaaagtcc agccagtttt atcaaaaggt gttagggcta gaggataaat
     9001 tcagtgtgcc tagcgctagt tggattgcgg ctggagatta ccatcatcat ttagcagtta
     9061 acgaatgggg aggaaaaggt ctggcttcgc gtaagcaagg cttaccaggt ttagcctact
     9121 atgtcatcga agtcgcacat aaaaaagaac tattaacgat tgcccaacga gcacaagaag
     9181 ttgacgcacc aatcaaatgg atgacatcga gccaattgga aatcacagac tcagatggca
     9241 tcgtgacccg tattcgttta gcaaggtaaa attaggaatt tttaataagt tataagtatt
     9301 gctagtttgc tttttgaacg gtctttggta acagaactgc gtgagctgga tgggcgagaa
     9361 ttttgacaaa gaagctgatt agagatataa tgaagaaaaa tcgagcaagg aaaaagaaat
     9421 gacaaattca aagtatatta cttgtttgaa acgttcagag ggccagttgt gtgggattca
     9481 aaagatgatt gaaggagatc gtgactgtgc tgatattgtg acgcaactga cagcagtgag
     9541 atctagtgta gagcgcgtga ttgagatgat aattaccgaa aatcttactg aatgtatcaa
     9601 tcagccacta gatgattctg aagctcaaaa ggaacgccta gaaaaggcta tccgatactt
     9661 gattaaacgg aaataaatga atgttttgag acgatatgtg caaaggtcta ttaactatta
     9721 gaatagttta atgtgagtca aaagaagttg taaaaaatct ttaaaattaa aaaaacgcat
     9781 agtatcaggt gttgaatgta ctgaccccaa aaagttagat tttttctgtc taacttttgg
     9841 ggtgcagttc ataagaacct tggtaatatg cgttttatta tgtagagact tatactcttc
     9901 gaaaatctct tcaaaccacg tcagtttcac cttgccgtag atatggttac tgacttcgtc
     9961 agttctatct acaacctcaa aacagtgttt tgagctgact tcgtcagttc catccacaac
    10021 cttaaaacag tgttttgagc agcctgcggc taacttccta gtttgcactt taattttcat
    10081 tgagtattac ttcatcttct cctgaaattg agtttttgcc cagctcctct tgattattct
    10141 acttgaccgg gccaagcatt cataccacct tctacgttgg taacggtgag gccttgggag
    10201 ctgagaaatt ggcaggtaga tgcagagcgg actcctcctt gacaaataac atagtattca
    10261 tggtcaggtt tgagttcttt gtagccttgc tctaaggtac ttaatggaag atttttggca
    10321 tctggtgcat gtcctgcttg gaattcatgt gcttcacgga catcgataag ttctagattt
    10381 tcattttgat attttccata aaagtcagcc atgctgatac tagtttccat attctactcc
    10441 ttctgggtta gtcattttgt atagtgaata agcgccgtca aggttttgga cggtaaatcc
    10501 tgcttgcttg aggataggct ctgcgatata gctgcgcaaa ccactgtggc agctgacgat
    10561 gtaggcttgt ttttctcctc agttcatcag cttcctcctt gacactttgt cagattttga
    10621 tacaatagta caaaattaga ggaggcaggc tatgattcag aaacatgcga ttcctatttt
    10681 agagtttgat gacaatcctc aggcggttat catgcccaat cacgaggggc tggacttgca
    10741 gttgccaaag aagtgtgttt atgcattttt aggtgaggag attgaccgct atgcaaggga
    10801 agtaggggcg gattgtgtcg gcgaattcgt ttctgctacc aagacctatc cagtctatgt
    10861 catcaactac aagggtgagg aggtctgtct ggctcaggct cctgttggct ccgctccagc
    10921 agcccagttt atggattggt tgattggcta tggtgtggag cagattatct ctactgggac
    10981 ctgtggtgtg ctagccgata tagaggaaaa tgcctttcta gtccctgttc gcgctctgcg
    11041 agatgaggga gccagttacc actatgtggc accttgtcgt tatatggaaa tgcagccaga
    11101 ggctattgct gctattgagg aagttttgga agacagaggg attccttatg aagaagtcat
    11161 gacctggacg acagacggtt tttaccgaga aacggctgaa aaggtggctt atcgtaagga
    11221 agaaggctgt gctgttgtgg agatggagtg ttctgctctt gcggcagtag ctcaattgcg
    11281 tggggttctc tggggtgaat tgttgttcac agcagattct ctagcggact tggaccagta
    11341 cgacagtcgt gactggggct cggaagcttt taataaggcg ctagaactga gtttagcaag
    11401 tgttcaccac ctttagttgt actggcaaag gatttgtttt atcataaaat gtctagctca
    11461 tacttttcaa aaatatgttt aaacgaggtc accttcctct tgtcctaggc atgttgaggt
    11521 tgggaaaaat ctttaaaatc agaaaaacgt atcatatcag gtgatgaaaa ctttgatact
    11581 atgcgtttta tgttggtaag atttagagtg agatgaaatg atactcttcg aaaatctttt
    11641 caaaccaggt cagcttcacc ttgccgtata gatgttactg acttcgtcag ttctatctgc
    11701 aacctcaaaa cagtgttttg agcaacctgt gactagcttt ctaagcgatg ccttggttct
    11761 cattgcctat aatcaaaaag agaaattttc tcctgaaaag catatagagt agctggcgtt
    11821 aaaagctcct gtcttgcttt tttgacctat agtcacatct atcaagtatt gttcttgcct
    11881 aagctatcaa taaaaaggtg gcatttttta ggcttggtgt tagtgaattt tgccttatcc
    11941 tatctaagtc atttcgagct ttttatggta caatggaaac atgttattca aattatctaa
    12001 ggaaaaaata gagctaggct tatctcgttt atcgccagcc cgtcgtattt ttttgagttt
    12061 tgccttggtc attttactag gctctcttct tttgagcttg ccctttgtcc aagttgaaag
    12121 ctcacgagcg acttattttg atcatctttt cactgctgtc tctgcagtct gtgtgacagg
    12181 tctctcaacc cttccagtag ctcacaccta taatatatgg ggccaaataa tctgtttgct
    12241 cttgattcag atcggtggtc tagggctcat gacctttatt ggggttttct atatccagag
    12301 caagcaaaag cttagtcttc gtagccgtgc aactattcag gatagtttta gttatggaga
    12361 aactcgatct ttgagaaagt ttgtctattc tatttttctc acgacctttt tggttgagag
    12421 cttgggagct attttgctta gttttcgcct tattcctcaa cttggctggg gacgtggtct
    12481 ttttagttcc atttttctag cgatctcagc cttctgtaat gccggttttg ataatttagg
    12541 gagcaccagt ttatttgctt ttcagaccga tttactggtc aatctggtga ttgcaggctt
    12601 gattattaca ggcggccttg gttttatggt ctggtttgat ttggctggtc atgtaggaag
    12661 aaagaaaaaa ggacgtctgc actttcatac gaagcttgta ttattattga ctataggttt
    12721 gttgttattt ggaacggcaa ctactctctt tcttgagtgg aacaatgctg gaacgattgg
    12781 caatctccct gttgccgata aggttttagt tagctttttt caaacagtga cgatgcgaac
    12841 agctggcttt tctacgatag attatactca ggctcatcct gtgactcttt tgatttatat
    12901 cttacagatg tttctaggtg gggcacctgg aggaacagct gggggactca agattacgac
    12961 attttttgtc ctcttggtct ttgcacgaag tgagcttcta ggcttgcctc atgccaatgt
    13021 tgcgagacga acgattgcgc cgcgaacggt tcaaaaatcc tttagtgtct ttattatctt
    13081 tttgatgagc ttcttgatag gattgattct gctagggata acagccaaag gcaatcctcc
    13141 ctttatccac ctcatatttg aaactatttc agctcttagt acagttggtg taacggcaaa
    13201 tctgactcct gaccttggga aattggctct cagtgttatc atgccactta tgtttatggg
    13261 acgaattggt cccttgacct tgtttgttag cttggcagat taccatccag aaaagaaaga
    13321 tatgattcac tatatgaaag cagatattag tattggttaa gaaaggaaag agcatgtcag
    13381 atcgtacgat tggaattttg ggcttgggaa tttttgggag cagtgtccta gctgccctag
    13441 ccaagcagga tatgaatatt atcgctattg atgaccacgc agagcgcatc aatcagtttg
    13501 agccagtttt ggcgcgtgga gtgattggtg acatcacaga tgaagaatta ttgagatcag
    13561 cagggattga tacctgcgat accgttgtag tcgcgacagg tgaaaatctg gagtcgagtg
    13621 tgcttgcggt tatgcactgt aagagtttgg gggtaccgac tgttattgct aaggtcaaaa
    13681 gtcagaccgc taagaaagtg ctagaaaaga ttggagctga ctcggttatc tcgccagagt
    13741 atgaaatggg gcagtctcta gcacagacca ttcttttcca taatagtgtt gatgtctttc
    13801 agttggataa aaatgtgtct atcgtggaga tgaaaattcc tcagtcttgg gcaggtcaaa
    13861 gtctgagtaa attagacctc cgtggcaaat acaatctgaa tattttgggt ttccgagagc
    13921 aggaaaattc cccattggat gttgaatttg gaccagatga cctcttgaaa gcagatacct
    13981 atattttggc agtcatcaac aaccagtatt tggataccct agtagcattg aattcgtaaa
    14041 gagggatgac ccctcttttt tgatgcccaa gatggcaaat agagacagaa gccccttgtc
    14101 ttctagtaaa agttcttcaa aggctggact ttatggtaaa atagaaggaa gtgacaagag
    14161 agagtaatac tcaatgaaaa tcaaagatca aactaggaaa ctagctgcgg gctgctcaaa
    14221 acactgtttt gaggttgtgg atagaactga cgaagtcagc tcaaaacact gttttgaggt
    14281 tgcagataga actgacgaag tcagtaacat ctatacggca aggcgacgtt gacgcggttt
    14341 gaagagattt tcgaagagta taagaaaaaa tcagtcccct aaaggagcag attatgaagt
    14401 tattgtctat cgccatttct agctataatg cagcagccta tcttcattac tgtgtggagt
    14461 cgctagtgat tggtggtgag caagttggga ttttgattat caatgacggg tctcaggatc
    14521 agactcagga aatcgctgag tgtttagcta gcaagtatcc taatatcgtt agagccatct
    14581 atcaggaaaa taaaggccat ggcggtgcgg tcaatcgtgg cttggcagag gcttctgggc
    14641 gctattttaa agtagttgac agtgatgact gggtggatcc tcgtgcctac ttgaaaattc
    14701 ttgaaacctt gcaggaattt gagagcaagg gtcaagaggt ggatgtcttt gtgaccaatt
    14761 ttgtctatga aaaggaaggt cagtcttgta agaagagtat gagttacgat tcagtcttgc
    14821 ctgttcggca gatttttggc tgggaccagg tcggaaattt ctccaaaggc cagtatatca
    14881 tgatgcactc gctgatttat cggacagatt tgttgcgtgc tagccagttc taattgcctg
    14941 aacatacttt ttatgtcgat aatctctttg tctttacgcc ccttcagcag gtcaagacca
    15001 tgtactatct gcctgtcgat ttctatcgtt atttgattgg gcgtgaggac cagtctgtca
    15061 atgagcaagt gatgattaag tgcattgacc agcaactcaa ggtcaatcga ctcttggtag
    15121 accaacttga tttgtcccaa gtgagtcatc ccaaaatgcg agaatatctg ctgaatcata
    15181 ttgaaatcac gacggtgatt tccagtaccc tgctcaaccg atctggaaca gcggagcatc
    15241 tggcaaaaaa acgccaattg tggacctata ttcagcagaa aaatccagaa gtctttcagg
    15301 ctattcgtaa gaccatgttg agccgtttga ccaaacattc tgtcttgcca gatcgcaaac
    15361 tgtccaatgt cgtctatcaa atcaccaaat ctgtttatgg atttaattaa tataagtgtt
    15421 ttataagagg gatttaagaa aaattttaac tttttcttag tcctttttaa tttcaggaga
    15481 ttatactaga gtcatcaaat aaagaaagac tctaaggaga atcctatgaa attcaatcca
    15541 aatcaaagat atactcgttg gtctattcgc cgtctcagtg tcggtgttgc ctcagttgtt
    15601 gtggctagtg gcttctttgt cctagttggt cagccaagtt ctgtacgtgc cgatgtggtc
    15661 aatccgaccc caggtcaagt cttacctgaa gagacatcgg gaacgaaaga gggtgactta
    15721 tcagaaaaac caggagacac cgttctcact caagcgaaac ctgagggcgt tactggaaat
    15781 acgaattcac ttccgacacc tacagaaaga actgaagtga gcgaggaaac aaactcttct
    15841 agtctggata cactttttga aaaagatgaa gaagctcaaa aaaatccaga gctaacagat
    15901 gtcttaaaag aaactgtaga tacagctgat gtggatggga cacaagcaag tccagcagaa
    15961 actactcctg aacaagtaaa aggtggagtg aaagaaaata caaaagacag catcgatgtt
    16021 cctgctgctt atcttgaaaa agctgaaggg aaaggtcctt tcactgccgg tgtaaaccaa
    16081 gtaattcctt atgaactatt cgctggtgat ggtatgttaa ctcgtctatt actaaaagct
    16141 tcggataatg ctccttggtc tgacaatggt actgctaaaa atcctgcttt acctcctctt
    16201 gaaggattaa caaaagggaa atacttctat gaagtagact taaatggcaa tactgttggt
    16261 aaacaaggtc aagctttaat tgatcaactt cgcgctaatg gtactcaaac ttataaagct
    16321 actgttaaag tttacggaaa taaagacggt aaagctgact tgactaatct agttgctact
    16381 aaaaatgtag acatcaacat caatggatta gttgctaaag aaactgtcga aaaagctgtt
    16441 aaagataacg ttaaagacag tatcgatgtt ccagcagcct acttagaaaa agccaagggt
    16501 gaaggtccat tcacagcagg tgtcaaccat gtgattccat acgaactctt cgcaggtgat
    16561 ggcatgttg
//
LOCUS       DBERMR010000045        15263 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.045, whole genome shotgun sequence.
ACCESSION   DBERMR010000045 DBERMR010000000
VERSION     DBERMR010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 15263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15263
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.045"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            73..720
                     /locus_tag="ACL8A1_001636"
     CDS             73..720
                     /locus_tag="ACL8A1_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HGS1202377.1"
                     /translation="MDFEYFYNREAERFNFLKVPEILVDREEFRGLSAEAIILYSILL
                     KQTGMSFKNNWIDKEGRVFIYFTVEEIMKRRNISKPTAIKTLDELDVKKGIGLIERVR
                     LGLGKPNIIYVKDFMSIFQVKENDLQKSKNLTSEVKDFNLRSKENELQEVKNLDSNYI
                     ENNKSKYSKREYSFGENGLGTFQNVFLAAEDISDLQIIMNSQLENYIRLPAKLES"
     gene            complement(709..1469)
                     /locus_tag="ACL8A1_001637"
                     /pseudo
     CDS             complement(709..1469)
                     /locus_tag="ACL8A1_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            1446..1718
                     /locus_tag="ACL8A1_001638"
     CDS             1446..1718
                     /locus_tag="ACL8A1_001638"
                     /inference="COORDINATES: protein motif:HMM:NF036719.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HGS1202378.1"
                     /translation="MFTCFIIHRTTIPYFVSQEVYWKVRNIEAEAIRRNCERGAIFSG
                     KIKYHEDSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            1963..3297
                     /gene="trmFO"
                     /locus_tag="ACL8A1_001639"
     CDS             1963..3297
                     /gene="trmFO"
                     /locus_tag="ACL8A1_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HGS1202379.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVVNHPLIEVVRDEITELPTDVITVVATGPLTSDALAEKIHALNNGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            3381..4118
                     /gene="pyrH"
                     /locus_tag="ACL8A1_001640"
     CDS             3381..4118
                     /gene="pyrH"
                     /locus_tag="ACL8A1_001640"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HGS1202380.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            4127..4684
                     /gene="frr"
                     /locus_tag="ACL8A1_001641"
     CDS             4127..4684
                     /gene="frr"
                     /locus_tag="ACL8A1_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HGS1202381.1"
                     /translation="MANIIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            4744..5598
                     /gene="cvfB"
                     /locus_tag="ACL8A1_001642"
     CDS             4744..5598
                     /gene="cvfB"
                     /locus_tag="ACL8A1_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HGS1202382.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSFKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            5607..5822
                     /locus_tag="ACL8A1_001643"
     CDS             5607..5822
                     /locus_tag="ACL8A1_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HGS1202383.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            5908..6876
                     /locus_tag="ACL8A1_001644"
     CDS             5908..6876
                     /locus_tag="ACL8A1_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HGS1202384.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            7069..7569
                     /locus_tag="ACL8A1_001645"
     CDS             7069..7569
                     /locus_tag="ACL8A1_001645"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1202385.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGFDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIV
                     EQSLED"
     gene            7572..7897
                     /locus_tag="ACL8A1_001646"
                     /pseudo
     CDS             7572..7897
                     /locus_tag="ACL8A1_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003032802.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(8198..9309)
                     /gene="ald"
                     /locus_tag="ACL8A1_001647"
                     /pseudo
     CDS             complement(8198..9309)
                     /gene="ald"
                     /locus_tag="ACL8A1_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003018852.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
     gene            9486..10055
                     /locus_tag="ACL8A1_001648"
     CDS             9486..10055
                     /locus_tag="ACL8A1_001648"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1202386.1"
                     /translation="MESIFVKLAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKSNGQFIGTIDLHKIDSVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKDNPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            10123..10773
                     /locus_tag="ACL8A1_001649"
     CDS             10123..10773
                     /locus_tag="ACL8A1_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HGS1202387.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     VEVAAVSKDLVSEKEVNKEEKEEPVEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSISKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD"
     gene            10757..12997
                     /locus_tag="ACL8A1_001650"
     CDS             10757..12997
                     /locus_tag="ACL8A1_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HGS1202388.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCL
                     FIQFPWKSAGKVLIICGIFGFWFVFQNWQQSQASQNLADSVERVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVTSRPLVFGQPNAWFLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     LEVTKAFHVGEILVSKGSLKQKEFVAELQTTQTKVRSMTVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGVDSW
                     KIESVR"
     gene            13176..13364
                     /locus_tag="ACL8A1_001651"
     CDS             13176..13364
                     /locus_tag="ACL8A1_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202389.1"
                     /translation="MTATKSYKYDWNTVLEYSTNYHDHQYAWIPELSRYYSYSEYKVG
                     GGWNYARYKVINYYTGSY"
     gene            13396..13983
                     /locus_tag="ACL8A1_001652"
     CDS             13396..13983
                     /locus_tag="ACL8A1_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS1202390.1"
                     /translation="MLNLTHVTLKTRQVILQDADFTFKKGRIYGLLAINGSGKTTLFR
                     AMSKLLPLSSGHIAVPPSLFYYESVEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            13987..15171
                     /locus_tag="ACL8A1_001653"
     CDS             13987..15171
                     /locus_tag="ACL8A1_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1202391.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILLLFASVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLPKQIDNVNLNWSMGMVLLPCLIIFLLVGILFIESWGSSRKKEVFN
                     RS"
BASE COUNT         4583 a         2823 c         3410 g         4447 t
ORIGIN      
        1 gttaccaaag gcgatagaag aaaaactatc gtctttttct ttgcaaattt ttaagaaggg
       61 aggtgatctt gcatggactt tgaatatttt tataacagag aagcggaaag atttaacttc
      121 ttaaaagtac cggagatatt agttgataga gaagaatttc ggggcttatc agcagaagca
      181 attatccttt attccatact tcttaaacag acaggaatgt catttaagaa taactggata
      241 gacaaggaag gcagagtatt tatctatttt actgtcgaag aaattatgaa aagaagaaat
      301 atctcaaagc caactgccat aaaaacatta gatgagcttg atgtaaaaaa aggaatagga
      361 ctgatcgaaa gagtaaggct tggacttggt aagccgaaca tcatttatgt taaagacttt
      421 atgagtatat ttcaggtaaa agaaaatgac ttacagaagt caaaaaactt aacttcagaa
      481 gtaaaagatt ttaacctcag aagtaaagaa aatgaacttc aagaggttaa gaaccttgac
      541 tctaactata tagagaataa taagagtaag tatagtaaga gagaatatag ttttggtgaa
      601 aacggacttg gaacatttca aaatgtgttt ttagctgctg aagatatatc ggatttacaa
      661 atcataatga actcacagct tgagaattac attagacttc ctgcaaaact agaatcctag
      721 ttcatgattg ataataccag caatcaaatt cattcgtaat ccgaagcgtt tacgatgatt
      781 tcgataggtt gttgaaaaca ttttaaacgt ttttactttg gcaaagatgt tctcaacctt
      841 gcttctctcc ttagatagcg catggttaca ggctttatct tcagctgtta gcggcttgag
      901 tttgctggat ttacgtggag tttgtgcttg aggatatatc ttcatgagcc cttgataacc
      961 actgtcggcc aagattttac cagcttgtcc gatatttctg caactcattt tgaacaactt
     1021 catatcatga ctatagttca cagcgatatc caaagaaaca attctccctt gacttgtgac
     1081 aatcgcctga gccttcatag cgtgggattt ctttttactt ccgtcacatc aatcattacc
     1141 gtgtcctcag aactgagagg agttcttgaa atcgtaacac cactttgaac aagagttact
     1201 tcaacccatt ggctccgacg gattaagttg ctttcgtgaa taccaaaatc agccgcaatt
     1261 tgttcataag ttcgatattc tcgcacatat tgaagagtga ccataagaag gtcttctagg
     1321 cttaatttag gttttcgtcc accttttgcg tgtttaagtt gataagctgt ttttaataca
     1381 gctaatatct cttcaaaagt cgtgcgctga acaccaacaa gacgcttaaa tcgtgcatca
     1441 gttagttgtt tacttgcttc atcattcata gaactactat accatatttt gtttcgcagg
     1501 aagtctattg gaaagtaaga aatattgaag ctgaggctat tagaagaaat tgtgagcgtg
     1561 gtgctatttt ttcaggtaaa ataaaatatc acgaagattc acagtttaaa ggagatcact
     1621 atgttgaatg ttatgctgtt ttagataata cggttatagc aagagataga ataacagtcc
     1681 ctatcgatcc gttatgtgga aaagatttta tagagtagca tataattgat tcttaactgg
     1741 aatactcact atctctttac atcaagaaaa tgactaaaca gggaagtttg ccttcttccc
     1801 tttttttgtt atactagtag aagaaaaaat agaaagattt gtgggagtga aaaagccctg
     1861 tggacttttt cagcctgagc caagaaactc gaaagcttgt aagtctgatt ggctttttca
     1921 atgtgaatct taacttcaca ctcccaaaga ggtattagtg tcgtgtctca atcttatatc
     1981 aatgttatcg gtgctggttt ggcaggttct gaagcagctt accaaatcgc agagcgtggt
     2041 attccagtta aactatatga aatgcgtggt gtcaagtcta caccccagca taaaacagac
     2101 aattttgctg agttggtttg ttccaattct ttgcgtgggg atgctttgac aaatgcagtt
     2161 ggtcttctca aggaagaaat gcgtcgcttg ggttctgtta tcttggaatc tgctgaggct
     2221 acccgtgttc ctgccggtgg agcccttgcg gtggatcgtg atggtttctc tcaaatggtg
     2281 accgaaaaag tggtcaacca ccccttgatt gaagtggtgc gtgatgaaat tacagaattg
     2341 ccaacagatg ttattacagt tgtggcgact ggtcccttga ctagcgatgc cctggctgaa
     2401 aagattcatg cccttaataa tggcgatggc ttctatttct acgatgcagc agcgcctatt
     2461 atcgatgtca acactatcga tatgagtaag gtctatctca aatctcgtta tgataaggga
     2521 gaagcagcct atctcaatgc tccaatgacc aagcaagagt ttatggattt ccatgaagcc
     2581 ttggtcaatg cggaagaagc accgctcaat tcttttgaaa aagaaaagta ctttgaagga
     2641 tgtatgccaa tcgaagttat ggccaaacgt ggcattaaaa ctatgcttta tggccctatg
     2701 aagccagtcg gtcttgagta tccggacgac tacacaggac ctcgtgatgg agaatttaaa
     2761 acaccttatg cggttgtgca acttcgtcag gataatgcag ctggtagcct atacaatatt
     2821 gttggtttcc agacccacct caaatgggga gaacaaaagc gtgtcttcca aatgattccg
     2881 ggtcttgaaa atgcggagtt tgtccgttat ggtgtcatgc atcgcaattc ttacatggat
     2941 tcaccaaatc ttcttgagca gacttaccgt tccaagaaac aaccaaatct cttctttgct
     3001 ggtcagatga cgggggtgga aggctatgtt gagtcggcgg cttcgggctt agttgcggga
     3061 attaacgcag ctcgtctctt caaggaagaa agcgaggtta ttttccccga gacgacagcg
     3121 attggaagct tagctcatta cattacccat gccgacagca aacatttcca accaatgaat
     3181 gtcaattttg ggatcatcaa ggagttggaa ggcgagcgta tccgtgataa gaaggctcgt
     3241 tatgaaaaaa ttgcagagcg tgcccttgcc gacttagagg aatttttgac tgtctaattt
     3301 ttttgaaaga attgctcatg atactataaa aatcttagaa attgtgataa aataggtagg
     3361 atgaaagaag gagagtgaaa atggcgaatc ccaagtataa acgtatttta atcaagttat
     3421 caggtgaagc ccttgccggt gaacgtggcg tagggattga tatccaaaca gttcaaacaa
     3481 tcgcaaaaga gattcaagaa gttcatagct taggtatcga aattgccctt gttattggtg
     3541 gaggaaatct ctggcgtgga gaacctgcag cagaagcagg tatggaccgt gttcaggcag
     3601 attacactgg aatgcttggg actgttatga atgctcttgt gatggcagat tcattgcaac
     3661 aagttggggt tgatacgcgt gtacaaacag ctattgctat gcaacaagtg gcagagcctt
     3721 atgtccgtgg acgtgccctt cgtcaccttg aaaaaggccg tatcgttatc tttggtgctg
     3781 gaattggttc accatacttc tcgacagata caacagcggc ccttcgcgca gctgaaatcg
     3841 aagcagatgc catcctcatg gctaaaaatg gcgtcgatgg tgtgtacaat gccgatccta
     3901 agaaggacaa gacagccgtt aagtttgaag aattgaccca ccgtgatgtt atcaacaaag
     3961 gtcttcgtat catggactca acagcctcaa ccctctcaat ggacaacgac attgacttgg
     4021 ttgtcttcaa catgaaccaa tcaggcaaca tcaaacgtgt cgtatttggt gaaaatatcg
     4081 gaacaacagt ttcaaataat atcgaagaaa aggaataaga aagaatatgg ctaacataat
     4141 tattgaaaaa gctaaagaga gaatgaccca gtctcaccaa tcacttgctc gtgaatttgg
     4201 tggtatccgt gctggtcgtg ccaatgcaag cttgcttgac cgtgtacatg tagaatacta
     4261 tggagtcgaa actcctctta accaaatcgc ttcaattacg attccagaag cgcgtgtttt
     4321 gttggtaaca ccatttgaca agtcttcatt gaaagacatc gaacgtgcct tgaacgcttc
     4381 tgatcttggt atcacaccgg ctaatgacgg ttctgtgatt cgcttggtta tcccagctct
     4441 tacagaagaa actcgtcgtg accttgctaa agaagtgaag aaggtcggcg aaaatgctaa
     4501 agtggctgtc cgcaatatcc gtcgcgatgc tatggacgaa gctaagaaac aagaaaaagc
     4561 aaaagaaatc actgaagacg aattgaagac tcttgaaaaa gatattcaaa aagtaacaga
     4621 cgatgctgtt aaacacatcg acgacatgac tgctaacaaa gagaaagaac ttttggaagt
     4681 ctaaaaataa acagaaaaac tcagttggca ttgctggctg agttttattc gaaagaagga
     4741 aatatgaata caaatcttgc aagttttatc gttggactga tcatcgatga aaatgaccgt
     4801 ttttactttg tgcaaaagga tggtcaaacc tatgctcttg ctaaggaaga gggccaacat
     4861 acagtagggg atacggtcaa aggttttgca tacacggata tgaagcaaaa actccgcctg
     4921 acaaccttag aagtgactgc cactcaggac caatttggtt ggggacgtgt cacagaggtt
     4981 cgtaaggact tgggtgtctt tgtggataca ggccttcctg acaaggaaat cgttgtgtca
     5041 ctcgatattc tccctgagct caaggaactc tggcctaaga agggcgacca actctacatc
     5101 cgtcttgaag tggataagaa agaccgtatc tggggcctct tggcttatca agaagacttc
     5161 caacgtctcg ctcgtcctgc ctacaacaac atgcagaacc aaaactggcc agccattgtt
     5221 taccgtctca agctgtcagg aacttttgtt tacctaccag aaaataatat gcttggtttt
     5281 attcatccta gcgagcgtta cgcagagcca cgtttggggc aagtattaga tgcgcgcgtt
     5341 attggtttcc gtgaagtgga ccgcactttg aacctctcct tcaaaccacg ctcctttgaa
     5401 atgttggaaa acgatgctca gatgattttg acttatttgg aaagcaatgg cggtttcatg
     5461 accttaaatg acaagtcatc tccagacgac atcaaggcaa cctttggcat ttctaaaggt
     5521 cagttcaaga aagctttagg tggtcttatg aaggctggta aaatcaagca ggaccagttt
     5581 ggtacagagt tgatttaggg aggcttatga gaaaatcatt ttacacttgg ctcatgaccg
     5641 agcgcaatcc taaaagtaac agtcccaaag caattttggc agacctcgct tttgaagagg
     5701 cagcttttcc aaaacataca gatgattttg atgaggtcag tcgctttttg gaggagcatg
     5761 ccagtttctc ttttaaccta ggagattttg acagcatttg gcaggaatat ctagaacact
     5821 agcatttatt cattgggttt gggctagtaa tttctccatc cctctgctat aataaaaaga
     5881 aataaaagga ttagagaggt tctttatttg aaggaacatt caatagacat tcaactgagt
     5941 catccagatg acctgtttca tctttttggt tccaatgagc gccatcttcg tttgatggaa
     6001 gaagagctgg atgtggtgat tcatgctcgt acggagattg tccaggtttt gggagaagag
     6061 tctgcttgtg aggaagcccg tcaggttatt caagctctca tggtcttggt gaaccgtggg
     6121 atgaccgttg gtacgccaga tgtggtgact gcaattagca tggtcaaaaa cgatgaaatt
     6181 gataagtttg tcgcccttta cgaagaagaa attatcaaag ataatactgg gaaaccgatc
     6241 cgcgtcaaaa ccttaggtca aaaactttat gtggacagtg tcaaacagca tgatgtgacc
     6301 tttggaattg ggcctgcagg gacggggaag acctttcttg cagttacttt ggcagtgact
     6361 gcccttaaac gtgggcaagt caagcggatt atcttgacac gtccagcggt ggaagcagga
     6421 gagagtctag gttttcttcc gggtgatctt aaggagaagg tagatcctta ccttagacct
     6481 gtttatgatg ccttgtatca gattctgggg aaagaccaaa caactcgact catggagcgt
     6541 gaaattatcg agattgcgcc ccttgcctat atgcgtggac ggaccttgga tgatgccttt
     6601 gtcattctcg atgaggcgca aaatacgacc atcatgcaga tgaagatgtt cttgactcgt
     6661 ttaggttttc attctaagat gattgtcaat ggagatatta gtcagattga cctgccacgt
     6721 aatgtcaagt ccggtttgat tgatgctcaa gagaaactca agaacatcca tcagattgac
     6781 tttgttcatt tttcagccaa ggatgtggtt cgccatcctg ttgtcgctca gattatccga
     6841 gcctatgaat attctactga agttgcacac gactgatttt gaggaagttc gcctgcaaaa
     6901 gaatatactt ttgagagagg aaaatccagt tgtataggct aaaggtttta tccaaaggtt
     6961 tgagacaacg attaggcacg atggaaagaa cttttatgtg gctgatgacg atcagtgcat
     7021 cttcctgtgt cataatcaca ggacacaaga aagtaggaat ttgaaaagat gattgaccaa
     7081 ctatctaagt attacagttg taggatacta actgaaaagg atattccaag tattttatct
     7141 ttatatgaaa gtaatcctct gtattttcag cattgtccac cagagccaaa ttttgcaact
     7201 gtaaaagagg acatgctttg tctacctgaa ggtaaagcta aggctgataa gttttttgtt
     7261 ggattttgga atggatttga ccttgtggct gttatggatt ttgtctatgc atatcctgat
     7321 gaggagactg tttttattgg tttgtttatg gttgatcaag cctatcagag gaaagggatt
     7381 ggtagtcata ttgtgacaga agcactagct tattttgcta agaactttcg aaaggcacgt
     7441 ttggcttatg ttaagggaaa tccgcaatct cagcattttt gggaaaagca gggctttaaa
     7501 tcaattggat gcgaggttaa gcaagaactc tatacggttg ttatcgttga acagagccta
     7561 gaagattaga aatggcatca agtaagaact atttggaatt tgttttggaa caattatcag
     7621 gattagatga tgtgacttac cgttccatga tgggggagta tattctttac ttccgcggca
     7681 agattattgg cggcatttat gacgatcgct tttagttaaa cccgtgcaag cagtcttaga
     7741 taagattgac caatcttctt ttgagtttcc atacaaaggt gccaaagaaa tgatttgagt
     7801 ggaagaactt gataataaga tgtttctata agacctgatt ttagctatgt ataaccaact
     7861 gccaacgccc aaacctaaaa agaaaaagca agggtgaacg aagtaaaaaa gaagtctgct
     7921 aaggccctgt ctttgcacgg gtaaaatttt atatataaaa agaagctggg actaaagagc
     7981 tcagcttcct ttggtttata taattgtcat tacaagacga agtggttggg cgaaactctg
     8041 ttgactttat tcaatttaga gtttcttatg cacaattgag tctggaacga aagtctccag
     8101 ttgcaaagta tacagtacaa taaaccaacg atgtaatagc tgatgacaca aagcacagtg
     8161 ggtaggactt gcgaagtcac ccttttcttt tcaaaattta tactaaatca ttgatatcag
     8221 tgtagtcacg attaagtcct tgagcaactg gtaggctagt caagtaacct tgataagtgg
     8281 tcacaccttg acgcaagcct tcatcttcag agattgcttg tgcgaatcct ttgccagcca
     8341 aagcttcgat ataaggaaga gtgacattgg ttagggcgat ggttgaagtg cgggcaaccg
     8401 caccagggat attggcaacg gcatagtgga gaacaccgtg tttttcatag acgggttcat
     8461 cgtgcgttgt cacacggtca gctgtttcga taaggccacc ttggtcaaca gcaatgtcaa
     8521 cgatacagag cctggacgca tttgtttgac catctcatct gtcaccaatt ccggtgcttt
     8581 tgcaccaggg atgagaatgg ctccaatcac cacatcagca tctctcatac ttgcttcaat
     8641 gttgaatgaa ttagatataa gagtttgaat ttgacttcca aagacttctt ctagaactga
     8701 gagacgcttg gcactaatat ctaaaatagt cacttgagca ccaagaccaa gggcgatgcg
     8761 ggcagcatgt gtaccgacga caccatcacc gatgatagtt acttttcctt ttggaacacc
     8821 tggtacacca ccaggtagaa caccagagcc accagcttgc ttagtaagga agtgagctcc
     8881 gatttaaaca gccatacgac ctgcaacctc actcatagga acgaggagcg gtagttgtcc
     8941 ttgattgtca cgaacagttt cataggcaat tcctgttgtt tttgctgcta acatagcatc
     9001 tgctaattct ggagcagcgg ccatgtgcaa gtaggtgaag agaagaagat cgtcgcgcaa
     9061 gtaaccgtat tcagaactta aaggttcttt tactttcaca accaactctg ctgcccaagc
     9121 ttcaccagca gtagcgacaa tctcagctcc ttgcttttga tagtcagcat cagtaaagcc
     9181 agaaccgaga ccagcatttg tttcgataag gacacgatga ccacgactaa ctaagctatg
     9241 aacacctgca ggtgtgaggg cgacacggtt ttcgttattt ttaatttctt ttgggattcc
     9301 gattaacatt gagataacct acctttcaat tgacggtctt gttttggttg tcacattcca
     9361 gttcataaat caaaaatgtg acggtttcat tgtatatgaa accgcttcaa aaatcaagaa
     9421 aaacttgtca tccaaatttt ttatgctaga ctagtgaaaa tcaagctttg attggttgga
     9481 aaagtatgga atcaattttt gtaaaacttg cccagtatcc gtctatagaa acggagcgtt
     9541 tattgcttag acctgtaact ttggatgatg cggaagcaat gtttgactat gcctcggaca
     9601 agggtaatac acgttacact tttccaacca atcaaagctt ggaagaaacc aagaataaca
     9661 ttgctcagtt ctatttggct aatcccttgg gacgttgggg aatagaacta aaaagcaatg
     9721 gtcagtttat tggaaccatt gacttgcaca agattgattc tgttcttaag aaggcagcta
     9781 ttggctacat tatcaataaa aagtattgga atcaaggatt aacgacagaa gccaatcgtg
     9841 ctgtgattga gctagctttt gagaagatag ggatgaataa gttgactgcc cttcacgata
     9901 aggacaatcc tgcgtcagga aaggtcatgg agaaatcagg catgcgtttt tcccatgcag
     9961 aaccatatgc ttgtatggac cagcatgaaa aaggccgaat cgtgacaaga gttcattatg
    10021 tcttgaccaa ggaagactat tttgcaaata aataagcagt tgaaaagaaa tttttcgact
    10081 gttttttctt cctcttacga ataatctaag agaggagaaa atatggaagc aattatcgag
    10141 aaaatcaaag agtataaaat catcgtcatc tgtactggtc tgggcttgct tgtaggcgga
    10201 tttttcctgc taaagccagc tccacaaaca cctgtaaagg aaacgaattt gcaggttgaa
    10261 gttgcagctg tttccaagga tttggtatcc gaaaaggaag tgaacaagga agagaaggaa
    10321 gaaccagttg aacaagatct aatcacagta gatgtcaaag gtgctgtcaa atcgccaggg
    10381 atttatgact tgcctgtagg tagtcgagtc aatgatgctg ttcagaaggc tggtggcttg
    10441 acagagcaag cagacagcaa gtcgctcaat ctagctcaga aagttagtga tgaggctctg
    10501 gtttacgttc ctactaaggg agaagaagca gttagtcaac agactggttc ggggacagct
    10561 tcttcaataa gcaaggaaaa gaaggtcaat ctcaacaagg ccagtctgga agaactcaag
    10621 caggtcaagg gactgggagg aaaacgagct caggacatta tcgaccatcg tgaggcaaat
    10681 ggcaagttca agtcagtaga cgagctcaag aaggtctctg gcattggtgg caaaacaata
    10741 gaaaagctta aagactatgt tacagtggat taagaatttc tctattcccc taatttacct
    10801 gagttttcta ttactttggc tttattacgc tattttctca gcatcttatc ttgctttgtt
    10861 gggctttgtt tttctgctag tctgtctctt tatccaattt ccgtggaaat ctgctggtaa
    10921 agttctaata atttgcggaa tctttggatt ttggtttgtt tttcaaaatt ggcaacagag
    10981 tcaagcgagt caaaatctgg cggattctgt tgaaagggta cggattttgc ctgatactat
    11041 taaggttaat ggtgatagtc tatcctttcg tggcaagtct aacggtcgtg ctttccaagt
    11101 ctattataaa ctccagtccg aggaggagaa agaagccttt caagctttaa ccgacctgca
    11161 tgagatagga ctagaaggga agctttcgga gccagaaggg cagagaaatt ttggtggctt
    11221 taattaccaa gcctatctga agactcaggg aatttaccag actctcaata tcaaaacaat
    11281 ccagtcactt caaaagattg gcagttggga tataggagaa aacttgtcca gtttacgtcg
    11341 aaaggctgtg gtttggatta agacgcactt tccagaccct atgcgcaatt acatgacagg
    11401 actcttgctg ggacatctgg acaccgactt tgaggagatg aatgagcttt attccagtct
    11461 aggaattatc cacctctttg ccctatctgg tatgcaggta ggttttttca tgaatggatt
    11521 taagaaactt ctcttgcgat tgggcttgac ccaagaaaag ttgaaatggc tgacttatcc
    11581 cttttccctt atctatgcgg gactaactgg attttcagca tcggttattc gcagtctctt
    11641 gcaaaagcta ctggctcaac atggggttaa gggcttggat aattttgcct tgacggtgct
    11701 tgtcctcttt attgtcatgc caaacttttt cttgacagca ggaggagtct tgtcctgcgc
    11761 ttatgctttt atcctgacca tgaccagcaa agaaggggag gggctcaagg ctgttactag
    11821 tgaaagtcta gtcatctcct tgggcatatt gcccattcta tccttctatt ttgcggaatt
    11881 tcaaccttgg tctatccttt tgacctttgt cttttccttt ctatttgact tgaccttctt
    11941 accgctcttg tctattttat ttgtcctttc ctttctctat ccagtcattc agctgaactt
    12001 tatctttgaa tggttggagg gcattattcg cttggtgtca caggtgacaa gtagacctct
    12061 ggtctttgga caacccaatg catggttttt aatcctattg ttaatttcct tggctttggt
    12121 ctatgatttg agaaaaaaca ttaaaaagct aacggtattg tgcttattga ttacagggct
    12181 ctttctcctg accaagcatc cactggaaaa tgaaatcacc atgctggatg tggggcaagg
    12241 agaaagtatt ttcctacggg atgtaactgg gaaaaccatt ctcatagatg taggtggtaa
    12301 ggcagaatct tataagaaaa tcgaaaaatg gcaagaaaag atgacgacca gcaatgccca
    12361 gcgaaccttg attccctatc tcaaaagtcg aggagtagct aagattgacc agctgatttt
    12421 gacaaatacg gacaaggaac atgtcggaga tttgttagag gtgaccaagg ctttccatgt
    12481 aggggagatt ttagtatcaa aaggcagttt gaagcagaag gaatttgtgg cagaactaca
    12541 gacgactcaa acaaaggtgc gtagtatgac agtaggggag aacttgccca tttttggaag
    12601 tcagttagaa gttctatctc caaggaaaat gggagatgga ggacacgatg acaccctagt
    12661 tctgtatggg aaattcttgg ataagcaatt tctcttcacg ggaaatttgg aggagaaagg
    12721 agagaaggac ttgctgaagc actatccaga cttgaaagta aatgttttga aagctagcca
    12781 acatggcaat aaaaaatcat caagtccagc ctttctagaa aaactcaaac cagagcttac
    12841 tcttatctca gttggaaaga gcaatcgaat gaaactcccc catcaggaaa cattgacacg
    12901 actggaaggt atcaatagca aagtttatcg aactgaccag caaggagcta tacgttttaa
    12961 gggggtggat agttggaaaa tcgaaagtgt tcgataggaa ggataaatgt tgtagattag
    13021 tgaaataaac taaaaatttg ttgcataata atgataaaaa cggtataatg aaaacgtatt
    13081 caatattgag gatataaaat cgttaaaaat cagcaaaagt tgttttatta gttagtttat
    13141 aatctattgg tcttcttcag tccagtgtat ctgctgtgac agccactaaa agttacaagt
    13201 atgattggaa tacggttttg gaatatagta ccaactatca cgaccatcag tatgcttgga
    13261 ttccggaatt gtctcgttat tacagctatt ctgagtataa agttggcgga ggctggaact
    13321 acgctcgtta taaggtcata aactactata ctggaagtta ttaatcctta aagagtgaga
    13381 aaaggagggc tggatatgtt aaatcttact catgttacct taaaaacgcg acaagtcatc
    13441 ttgcaagatg cggattttac ctttaaaaag ggtaggattt atggccttct tgctatcaat
    13501 ggctcgggaa agacgacact attccgagct atgagcaagt tgcttcccct tagtagtgga
    13561 cacatcgcag ttcctccttc tttgttttat tatgagagcg ttgaatggct ggatggaaac
    13621 ttaagtggga tggactacct tcgtctcata aaaaacatct ggaagtcaga cctaaacttg
    13681 agagatgaaa tcgcctactg ggaaatggct gactatatca gtcttcccat ccgcaagtat
    13741 tccttaggca tgaagcaacg cttggtgatt gctatgtatt ttctcagtca ggccaaatgc
    13801 tggctcatgg atgagattac aaatggctta gacgagtatt atcgacagaa gttttttgat
    13861 aggctagcac aaatcgatag acaagaacag ctggttcttt taagttccca ctataaggaa
    13921 gagttggttg atatctgcga tagagtagta accattcatc aggggcagat agaagaggtt
    13981 tagtttatga aagatgttag tctattttta ttgaaaaaag ttttcaaaag tcgtttaaac
    14041 tggattatct tacttttatt tgcatctgta ctcggtgtta ccttttattt aaatagtcag
    14101 actgcaaact cacacagctt ggagagcagg ttggaaagtc gcattgcagc caacgagagg
    14161 gctatcaatg aaaatgaaga gaaactctcc caaatgtctg ataccagctc ggaggaatac
    14221 cagtttgcta aaaataattt agacgtgcaa aaaaatcttt tgacgcgaaa gacagaaatt
    14281 ctgactttac taaaagaagg gcgctggaaa gaagcctact atctgcagtg gcaagatgaa
    14341 gagaagaatt atgaatttgt atcaaatgac ccgactgcta gctctggctt aaaaatgggg
    14401 gttgaccgcg aacggaagat ttaccaagcc ctgtatccct tgaacataaa agcacatact
    14461 ttggagtttc cgacccacgg gattgatcag attgtctgga ttttagaggt tatcatccca
    14521 agcttgtttg tggttgcgat tatttttatg ctaacacaac tatttgcaga aagatatcaa
    14581 aatcatctgg acacagctca cttatatcct gtttcaaaag tgacatttgc aatatcctct
    14641 cttggagttg gagtgggata tgtaactgtg ctgtttatcg gaatctgtgg cttttctttt
    14701 ctagtgggaa gtctgataag tggttttgga cagttagatt atccctaccc aatttatagc
    14761 ttagtgaatc aagaagtaac tattgggaaa atacaagatg tattatttcc tggcttgctc
    14821 ttagctttct tagcctttat cgtcattgtg gaagttgtgt acttgattgc ttactttttt
    14881 aagcaaaaaa tgcctgtcct ctttctttca ctcattggga ttgttggttt attgtttggc
    14941 atccaaacca ttcagcctct tcaaaggatt gcacatctga ttccctttac ttacttgcgt
    15001 tcagtggaga ttttatctgg aagattacct aagcaaattg ataatgtcaa tctaaattgg
    15061 agcatgggaa tggtcttact tccttgtctg attatctttt tgctagtggg gattctattt
    15121 attgaaagct ggggaagttc acgtaaaaag gaagttttta ataggtctta gcattcttat
    15181 aaggaagtta aggaaaaact aacatagaga gggaatcaac ttgattctct ctttttgatt
    15241 cgaaaaccaa accaaaatac aaa
//
LOCUS       DBERMR010000046        15050 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.046, whole genome shotgun sequence.
ACCESSION   DBERMR010000046 DBERMR010000000
VERSION     DBERMR010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 15050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15050
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.046"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..493
                     /locus_tag="ACL8A1_001654"
     CDS             <1..493
                     /locus_tag="ACL8A1_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HGS1202392.1"
                     /translation="LEDLLATVKYYVEHPNERPHSDNGFGNASDHVQRNKNGQADTNQ
                     TEKPQTEKPQTEKPEEETPREEKPQSEKPESPKPTEEPEEESPEESPEESEEPQVETE
                     KVKEKLREAEDLLGKIQDPIIKSNAKETLTGLKNNLLFGTQDNNTIMAEAEKLLALLK
                     ENK"
     gene            912..1292
                     /gene="dhaM"
                     /locus_tag="ACL8A1_001655"
     CDS             912..1292
                     /gene="dhaM"
                     /locus_tag="ACL8A1_001655"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HGS1202393.1"
                     /translation="MESIGIGLVIVSHSKHIAEGVVELISKVAKDVPITYVRGTEGGG
                     IGTSFEQVDRVVSENPADTLLAFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAYNA
                     AALLQAGAELSVIQTQLAELEINK"
     gene            1750..2463
                     /locus_tag="ACL8A1_001656"
     CDS             1750..2463
                     /locus_tag="ACL8A1_001656"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HGS1202394.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFAHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYNLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            2521..3123
                     /locus_tag="ACL8A1_001657"
     CDS             2521..3123
                     /locus_tag="ACL8A1_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202395.1"
                     /translation="MSEVDFNEAVNYEFTSDTCQLANSIYQSLFKFFDKKNFSGDLIF
                     TWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLNMNRNGCIGDFPHDFF
                     DTYLDHVAKYAYEQKVNNIKEYYPLKRAILHQENALYFRLFSNFDDFLEKNYLKTIWQ
                     VSKETPFSEMDFNMFKNISEKIIFERGSKMLNDLKSNYKK"
     gene            3261..3914
                     /locus_tag="ACL8A1_001658"
     CDS             3261..3914
                     /locus_tag="ACL8A1_001658"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HGS1202396.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGTIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            3924..4388
                     /locus_tag="ACL8A1_001659"
     CDS             3924..4388
                     /locus_tag="ACL8A1_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HGS1202397.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAVREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGM
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            4401..5669
                     /locus_tag="ACL8A1_001660"
     CDS             4401..5669
                     /locus_tag="ACL8A1_001660"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HGS1202398.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGHVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLG
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFAAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            5683..5943
                     /locus_tag="ACL8A1_001661"
     CDS             5683..5943
                     /locus_tag="ACL8A1_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202399.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            5954..7315
                     /gene="pdrM"
                     /locus_tag="ACL8A1_001662"
     CDS             5954..7315
                     /gene="pdrM"
                     /locus_tag="ACL8A1_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HGS1202400.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            7469..8437
                     /locus_tag="ACL8A1_001663"
     CDS             7469..8437
                     /locus_tag="ACL8A1_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HGS1202401.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            8453..9445
                     /locus_tag="ACL8A1_001664"
     CDS             8453..9445
                     /locus_tag="ACL8A1_001664"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HGS1202402.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVILVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            9663..10706
                     /locus_tag="ACL8A1_001665"
     CDS             9663..10706
                     /locus_tag="ACL8A1_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HGS1202403.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            10752..12455
                     /gene="lpdA"
                     /locus_tag="ACL8A1_001666"
     CDS             10752..12455
                     /gene="lpdA"
                     /locus_tag="ACL8A1_001666"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003001382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HGS1202404.1"
                     /translation="MALEVIMPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKV
                     SMELEAEEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTS
                     NDDGKSDDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTY
                     LHSAEIIENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVAV
                     HKGIGTITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNE
                     VPESLVIIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLRLILERKGM
                     TILTGTKLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCI
                     KVNEYMETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIY
                     TLPEVAAVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGV
                     HIIGPAAAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKK
                     K"
     gene            12519..13508
                     /locus_tag="ACL8A1_001667"
     CDS             12519..13508
                     /locus_tag="ACL8A1_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HGS1202405.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINRIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEAVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(13581..14651)
                     /gene="xerS"
                     /locus_tag="ACL8A1_001668"
     CDS             complement(13581..14651)
                     /gene="xerS"
                     /locus_tag="ACL8A1_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HGS1202406.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLVIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVSDEQKNALDSL"
BASE COUNT         4620 a         2720 c         3260 g         4450 t
ORIGIN      
        1 tcttgaggat cttttggcga ctgtcaagta ctatgtcgaa catccaaacg aacgtccgca
       61 ttcagataat ggttttggta acgctagcga ccatgttcaa agaaacaaaa atggtcaagc
      121 tgataccaat caaacggaga aacctcagac agagaaacct cagacagaaa aacctgagga
      181 agaaacccct cgagaagaga aaccgcaaag cgagaaacca gagtctccaa aaccaacaga
      241 ggaaccagaa gaagaatcac cagaagaatc accagaggaa tcagaagaac ctcaggtcga
      301 gactgaaaag gttaaagaaa aactgagaga ggctgaagat ttacttggaa aaatccagga
      361 tccaattatc aagtccaatg ccaaagagac tctcacagga ttaaaaaata atttactatt
      421 tggcacccag gacaacaata ctattatggc agaagctgaa aaactattgg ctttattaaa
      481 ggagaataag taaaggtagc agcattttct aactcctaaa aacaggatag gagaacggga
      541 aaacgaaaaa tgagagcaga atgtgagttc tagttctcat ttttttatga aaatgtgcaa
      601 aatatagtag attgaaatta gaatagtata cctctacttc taaaacattg ttagaaatcg
      661 atttgactgt cctgttctta tttcatttta ctatatctta acagatagtg taaataaaga
      721 taaactattt actggctaat taatcagtta aacactagtt aaggagtaat gataaaaaag
      781 aacaatacta ttattgatgg ccagtctgtt ggctcttgtc ttaggagcat gtggtttttt
      841 ggacatattg atcctggatc attctcatca ggattactct ttactgctat tttagaaact
      901 ggggtggttt gatggaaagt attggtattg gtcttgttat cgtttcacat tccaaacaca
      961 ttgcagaagg tgttgttgaa ctgattagta aagtagctaa agatgttccg attacttatg
     1021 taagaggaac cgagggcgga ggaattggaa cgagttttga acaagtagat agggttgttt
     1081 ccgaaaatcc agcagatact ttacttgcct tttttgacct aggttctgct aaaatgaact
     1141 taaaaatggt gactgatttc agtgataaaa gtatcatcat caacagggtt ccaattgtag
     1201 aaggtgccta taatgcagct gctcttcttc aggctggtgc agaactgtca gttattcaaa
     1261 cacagttagc ggagcttgaa atcaataaat aaggaatttt actataactc tttttatagg
     1321 taagctattg attatctcaa ctataaaatg ttaagtaaat gatttcaaaa tcagaaaggg
     1381 attgctttat gcagttcctt tttattttaa caggagtgaa actatagtgt ttctaaattg
     1441 tgaatcaatc aaaactgatt gtgatggggc tattctagct ttagaaacct tcaaaaatta
     1501 aaatttaagg caatcaatta cttggaagag tatgaaagca tttagtttat aggaattcta
     1561 ggtctagaat tacatatata tttatgaaga cggggtgttc gatagttagt attgttctat
     1621 tctgaaagat ttgagctgtc agttgtatag aaagtgttcg aattttttta agtgattaaa
     1681 ttagttaatt gtatgaggtg ctttatgata taatgttctt aatgaatttt cagaaaggaa
     1741 aacctcaaat tgttctacaa atttctactc ttcgacctcg accacactct tcttgatttt
     1801 gatgctgctg aggatgtggc tttgacccaa cttctaaaag aagaaggagt tgcggatatt
     1861 caggcttata aagattatta cgttcctatg aacaaggctc tctggaaaga cttggagctg
     1921 aagaaaatca gtaaacaaga gctggttaac acgcgctttt ctcgtttatt tgctcatttt
     1981 ggacaggaaa aagacggtag ttttcttgcc cagcgttacc aattttacct cgcccagcag
     2041 ggacaaacac tatcgggcgc tcatgatctc ttggacagcc tcattgagcg tgattataac
     2101 ttgtatgctg cgacaaatgg cattactgcc attcagacag gacgtttggc tcaatctggt
     2161 ctagcacctt atttcaatca agtctttatc tcagaacagt tgcaaactca aaagccggat
     2221 gctctttttt atgaaaagat tggccagcaa attgctggat ttagtaaaga aaagacgctg
     2281 atgattggag attctctaac cgccgacatt caaggtggca ataatgcggg gattgacact
     2341 atctggtata atcctcatca cctcgaaaat cacacacaag cccagccgac ttacgaagtc
     2401 tattcttacc aagacttgct ggattgttta gataaaaata ttcttgaaaa gatcacattt
     2461 taaaggagac gagctaatga ctacaaaaaa gctaatatta ctattgaaga gtacattgaa
     2521 atgtctgaag ttgattttaa tgaagctgtt aattatgaat ttacatctga cacttgtcaa
     2581 ttagcaaata gtatttatca atctcttttt aagttttttg ataagaaaaa tttctctggc
     2641 gatttaattt ttacttggaa atctccatca ttagtcaaag aaggggatta tattgggaga
     2701 agggattcac aagtagataa tcttagagta ataggaaata tatttccgaa ttatcttact
     2761 aatcgaaaat atagcctcaa tatgaatcgt aatggctgta taggagattt tcctcatgac
     2821 ttttttgata catacctaga tcatgtagca aaatatgcct acgaacaaaa agttaataat
     2881 attaaagagt attatccttt aaaaagagcg attttacacc aagagaatgc attgtatttt
     2941 cgattgtttt ctaattttga cgacttttta gaaaaaaatt atttaaagac tatatggcaa
     3001 gtttctaaag aaactccttt ttctgaaatg gattttaata tgtttaaaaa tatttcagaa
     3061 aagataatct tcgaaagagg tagtaaaatg cttaatgatt taaaaagtaa ttataaaaaa
     3121 tagccagtct ttataaactg tattcacgta ttcaaatcgt atatatagtc gatttattaa
     3181 atctttagat aaactgtaaa aagtggtttc catagccact ttttgctata atagaggcag
     3241 taaaaatgaa ggagtcagct atggaacact cgtcttggca cgctttgatt aaggcgcaat
     3301 tacctgaggg ttatttcggg aaaatcaatc agtttatgga gcaggtctat tctcagggga
     3361 ctatttatcc acccaaggaa aaggtttttc aggctctctt gacaacactg cttgaagaag
     3421 ttaaggtggt aattctaggg caagacccct accacggacc aggtcaagcg cagggcttga
     3481 gtttttctgt acctgactct atcccagctc caccatcctt gcaaaatatc ttgaaagaat
     3541 tgtcagatga tatcggagtt aagaaatctc atgatttgac agcttgggct gagcaaggag
     3601 tcttgcttct taatgcttgt ttgactgttc ctgctggaca ggccaatggt catgctggtc
     3661 aaatctggga gccttttact gatgctgtga ttcaggtggt caatcatcta gatagaccag
     3721 tcgtttttgt actctgggga gcttatgcac gtaagaagaa ggcattagtt accaatcctc
     3781 atcacttgat tatcgaatca gcccatccaa gtcctttgtc ggtttataga ggattttggg
     3841 gttccaagcc cttttccaag gccaatgcat tcttaaaaga gacaggacaa gagccaatcg
     3901 attggcttag ataaggagaa aatatgcctc agttagcgac gatttgctac attgataatg
     3961 ggaaagaact gctcatgctc catcgtaata agaaacccaa tgatgtccat gaagggaaat
     4021 ggattggtgt gggtggtaag ctagagagag gagagacgcc ccaggaatgc gcggtacgtg
     4081 aaatccttga agaaacaggg ctcaaagcca agccagttct aaaaggtgtc atcacttttc
     4141 ctgaatttac accagattta gactggtaca cctatgtttt taaggtgacg gagtttgagg
     4201 gcgacttgat tgactgcaat gaggggatgc tagaatgggt tccctatgat gaggttttga
     4261 gcaagccgac ttgggaaggt gaccacacct ttgttgagtg gcttttagag gataaaccct
     4321 tcttttcagc caagtttgtt tatgatgggg ataaattgtt ggatacccaa gttgatttct
     4381 atgaataaag gagaaagcag atgctactaa tcaaaaatgg tcgtgtaatg gatcccaagt
     4441 ctggtttgga tcaagtttgt gatgtcttag ttcaagatgg gaaaattatc aaaattgcgc
     4501 ctgagatcac ggaagaagga gcagaaacga ttgatgctac tggtcatgtg gttgctcctg
     4561 gtttggtcga tattcatgtg catttccgtg aacctggtca aacacataaa gaagacattc
     4621 atactggtgc cctagcagcc gctgcaggtg gttttactac tgtcgtcatg atggctaata
     4681 ccagtccaac catttcagac gtggagactt tgcaagcagt tctccagtca gctgccaaag
     4741 agaagattaa tgtcaagaca gttgcgacca ttactaaaaa ctttaatggt aaaaacttga
     4801 ctgactttaa ggcactttta gaagctggtg cggttggttt ctctgatgac ggtattccgc
     4861 ttgagagcag taagattgtc aaggaagcca tggaggaagc caaaaaactc aataccttta
     4921 tcagccttca tgaggaagat ccaggtttga acggtgttct tggctttaat gaaaatattg
     4981 ctagagaaca tttccatatc tgtggtgcta ctggggtggc tgagtacgct atgatggcgc
     5041 gtgatgtcat gattgcctat gcaactaaag cccatgttca catccagcat ttgtctaagg
     5101 aagaaagtgt taaagtagtg gagtttgctc aggggttagg tgcagaagtc acagcagaag
     5161 tagcaccaca gcatttctct aagacagaag cacttctttt aacacaaggt agcaatgcta
     5221 agatgaatcc accgcttcgt ttggaatcag accgtcgtgc cgttatcgaa ggtctcaaat
     5281 caggtgtcat cacagttatt gcgactgacc acgcgcctca tcatgtagat gaaaaaaatg
     5341 ttgaggatat taccaaagcg ccatctggta tgactggctt agaaacatcc ctgtctctcg
     5401 gcttgaccta tttagtagaa gctggtgagt tgagcttgat ggaattactt gaaaaaatga
     5461 catacaaccc agccaagctt tacaactttg aagcaggtta cttggctgag aatggtccag
     5521 cagatatcac tatttttgct gccaaggctg accgccttgt ggactcccat tttgcttcca
     5581 aagcagctaa ttcaccattc atcggtgaaa ccttaaaagg gcaggttaaa tataccatct
     5641 gtaagggaca aatcgtctat caagcttgat aggagttaga atatgaatta ttttcgaaaa
     5701 cgtagggaga gacaagcgaa atctaatagt ggaatttact gtcccgcagc taatcgttcc
     5761 aaaattcaat atgaaactaa agaaaaagct gaccatgcgg ttcaatatga taacggaggt
     5821 ttagttagaa gctattattg tcgtacatgt gcgtgttggc atactacttc aaaatctaat
     5881 aaacctccgc taagtttgaa aaactatggt ctgaaactct ttggtttaga taaatcagaa
     5941 tagaaagaga agtatgtata agacaaagtg tttacgagag aagttagtat tatttttaaa
     6001 aattttcttc ccaatcctga tctaccaatt tgccaattat tctgcctctt ttgttgatac
     6061 tgcaatgaca ggtcaataca acactatgga cttggctggt gtatctatgg caaccagtat
     6121 ctggaatcct ttctttacat ttctaacagg gattgtgtca gccttggtgc ctatcattgg
     6181 tcaccatctt ggtcgaggca aaaaggaaga agttgcgtct gatttttacc aatttattta
     6241 tttggccttg ggcctatctg tggtcttgct ggggatggta cttttcttgg caccaacaat
     6301 cttgaatcat attgggttag aagcagcagt agcggcagta gcggttcgct atctttggtt
     6361 tttatctatc gggattatcc ccttgttgct ctttagcgtc attcgttcct tgctggattc
     6421 gctgggcttg accaaactgt ccatgtacct catgcttttg ttactccctc tcaatagcgg
     6481 atttaactat ctcttgattt acggtgcctt tggtgttcca gaactgggag gggctggtgc
     6541 tggtttagga acatccttgg cctactgggt cttgcttggg atttctgttc tggttttatt
     6601 taaacaggag aagctcaaag ccttacacct tgagaaacga attccactta atatggataa
     6661 aattaaggaa ggagttcgtt taggtctgcc tattggggga actgtcttcg cggaagtggc
     6721 tatcttttca gtggttggct tgattatggc taagttttcg tccttgatta tagctagtca
     6781 ccagtcagct atgaactttt caagtcttat gtacgccttt cctatgagta tctcatcggc
     6841 tatggctatt gtcgtttcct atgaagtggg agccaagcga tttgatgatg cgaaaaccta
     6901 tattggtcta ggaagatgga ctgccctcat ttttgcggcc ttcaccctaa ccttccttta
     6961 catttttagg ggaaatgtgg ccagtcttta tggtaacgac ccaaaattta tcgatttgac
     7021 agcgcgtttt ttaacttata gtcttttctt ccagttagca gatacctttg cggcaccgct
     7081 tcagggaatt ttgcgggggt ataaggatac agttattcct ttttaccttg gtttgcttgg
     7141 ttattggggc gtagcaatcc ctgtggctac gctatttgat tccctaacag attttggagc
     7201 ttattcttac tggatcggct tgattattag tttgattgtg agtggggcgc tctaccgttg
     7261 gcgtttaact gtgattatga agagatttga atctttagca aaatccaaac gctaaaaagt
     7321 ttgtgaaaaa atatccttga caagaaaatc ccttgttttc acggggtttt cttttttatt
     7381 ttgtgaaatt tcttttagaa aaactttgac agtatttgcc aaaagcggta aaatagtaag
     7441 gtaagaagaa agttagaaag gcaagaagat gtcaacttta gataaaaatc ttttgctaga
     7501 aatgttccgt aagatggaag aaatccgtcg catggactta aaaattgcac aattagtaaa
     7561 gaaagggaaa gtgccaggaa tgacgcactt ttctgttggt gaagaggcag ctaacgtggg
     7621 ggctatgtta gctctcaatc cagatgatct gattacctca aaccaccgtg gacacgggca
     7681 agctattgct aaagggattg acctcaacgg aatgatggct gaaatccttg gtaaatacac
     7741 tggaacctgt aaagggaaag gtggatctat gcatatcgct gaccttgatg ctgggaacct
     7801 tggtgccaat ggtatcgtag gtggtggtat ggggatcgct gtcggtgcag ccctcagtca
     7861 gcaaatgcaa aataccggta aaatcgttgt ctgcttcttt ggagatggtg cgaccaatga
     7921 aggtgttttc cacgaagcag tgaacatggc ttctatctgg aacctgccag tcattttcta
     7981 ttgcattaac aacggttacg gtatctctgc ggatatcaag aaaatgacca atatagaaca
     8041 tatccatcaa cgtagcgccg cttatggaat tcctggaatg ttcatcgaag acggtaacaa
     8101 tgtcatcgat gtctatgaag gatttcagaa agctgtagac catgttcgca gtggcaatgg
     8161 tccagtcttg attgaaagtg taacttatcg ctggctcggt cactcatcat ctgaccctgg
     8221 taaatatcgt acgcgtgaag aagtggaatt gtggaaacaa aaagatccaa tcgaaaacct
     8281 ccgcaattac cttattgaaa ataacattgc aagtgccgaa gaattggaag aaatccaagc
     8341 gcaagtaaag gaagcagtag aagcttctgt taaatttgca gaggaaagcc cattcccacc
     8401 gcttgaatca gcctttgaag atatttacgc agactaagga gaaagagata aaatggaaac
     8461 aaaaacaatg tccttccgtg acaccattat ccttgctatg tctgaggaaa tgcgtcgcga
     8521 tgaaaatgtg ttcttgatgg gagaagacgt cggtgtcttc ggaggagact tcggtacttc
     8581 tgttggaatg cttgaagaat ttggtccaga acgtgtccgt gactgtccga tttctgaagc
     8641 tgccatctct ggagctgcag caggagcagc catgacagga cttcgtccaa tcgtcgatat
     8701 gaccttcatg gacttctctg ttattgccat ggacaatatc gtcaaccaag ctgctaaaac
     8761 acgttacatg tttggtggta aaggtcaggt tccaatgact gttcgttgtg cagctggtaa
     8821 cggagttggc tctgccgccc agcactcaca atctctagag tcttggttta ctcacattcc
     8881 aggacttaag gttgtggcac ctggaacacc tgcggacatg aaaggcctgc ttaagtcttc
     8941 tatccgtgat aacaacccag ttattatact tgagtacaag tcagaattta accaaaaagg
     9001 ggaagtgcca gttgatccag actacacaat tccacttggg gttggtgaaa tcaaacgcca
     9061 aggaacggat gtaacagttg ttacttatgg aaaaatgctt cgccgtgtgg ttcaagctgc
     9121 tgaagaatta gcagaagaag gaatttcagt tgaaattgtt gacccacgta ctcttgttcc
     9181 gcttgataag gatatcatca taaactcagt gaagaagact ggtaaggtta ttctggtcaa
     9241 cgatgcccac aaaacaagtg gctatatcgg tgaaatttca gctattattt cagaatcaga
     9301 agcatttgac tatctagatg caccaatccg ccgttgtgca ggagaagatg tgccgatgcc
     9361 ttatgcacaa aacctagaaa atgcaatgat tccaacagtt gaaagtatca aagatgcaat
     9421 ccgaaaaact tataacaaag aatagagtta cataagataa gttatgtaaa taaaataaag
     9481 ctgacgagaa actttgtatc ttttaggtgc agagctcttc gtcattaata tcttagatta
     9541 gagcacgggc taaaagatcg gaaaaaagat aaatctcctt ggcttcatcg aagccatcgt
     9601 cgatttccta tttttcagtc gcttttgacg ccctttgtat cataaataga attggagagg
     9661 tcatggctga tgacaagcta agagcgactc ctgcagctag aaagttagcg gatgatttag
     9721 ggataaacct ctacgacgtt tctggctcag gtgcaaacgg tcgtgtccac aaagaagacg
     9781 tggaaactta taaagacaca aacgtggttc gcatttcgcc acttgcaaaa cgaattgccc
     9841 tcgaacataa cattgcttgg caggaaatcc aaggaaccgg tcatcgtggt aaaatcatga
     9901 agaaggatgt tttggccctg cttcctgaaa atatcgaaaa cgatagcatc aaatctcctg
     9961 ctcagattga aaaagtggaa gaagtccctg ataacgtaac accatatggt gaaatcgagc
    10021 gtattccaat gacaccaatg cgtaaggtta ttgcccaacg catggttgaa tcttacttga
    10081 ctgcgccaac cttcaccctc aactatgaag ttgatatgac tgaaatgttg gctcttcgta
    10141 agaaggttct tgagccaatc atggaagcaa ctgggaagaa gactactgta acagaccttc
    10201 tttcgcttgc agttgttaag actcttatga aacacccata catcaacgct tcattgacag
    10261 aagatggcaa gactattatc actcacaact atgtcaatct tgccatggca gttgggatgg
    10321 ataatggatt gatgacacct gttgtttaca atgctgagaa gatgagtctt tcagaactgg
    10381 tggtagcctt taaggatgtt attggccgta ccttggatgg taaattggct ccaagtgagt
    10441 tacaaaattc aacattcaca atcagtaatt tgggaatgtt tggtgttcag tcctttggtc
    10501 cgattattaa ccaacccaac tcagctatcc ttggtgtcag ttcgacaatc gagaagccag
    10561 ttgtcgtcaa tggtgaaatt gtgattcgcc caatcatgag tttaggatta accattgacc
    10621 accgtgtcgt agatggtatg gctggtgcta agtttatgaa ggacttgaaa gagttgattg
    10681 aaacgccaat ctcaatgtta atttaagagc aagagtattc atttttaaga tatagataaa
    10741 ggaaggaaga catggcctta gaagtaatta tgccaaaagc cggcgtggat atgacagaag
    10801 gacaaatcgt ccaatggaat aaaaaagtcg gagaatttgt aaaagaagga gaaatccttt
    10861 tggaaatcat gactgataaa gtcagcatgg aattggaagc cgaagaagat gggtacttga
    10921 ttgccattct caaaggagat ggtgaaactg tccctgtaac ggaagttatc ggttaccttg
    10981 gtgaagaaag ggaaaacatc ccaacagctg gagcagcatc gccagaagct agccctgtac
    11041 ctgtagcaag tacctcaaac gatgatggta agagcgatga tgcttttgat atcgttgtga
    11101 ttggtggagg tcctgctggt tatgttgcag ccattaaagc tgcccaactc ggcggtaagg
    11161 ttgcccttgt tgagaaatct gaacttggtg gaacctgctt aaaccgtgga tgtattccaa
    11221 ccaagaccta ccttcatagc gctgaaatta ttgaaaatat cggtcatgct gcaaatcgtg
    11281 gtatcgtgat tgaaaatcct aacttcactg ttgatatgga aaaactttta gaaactaaat
    11341 ctaaagttgt taatactctt gttggtggtg ttgcaggact tcttcgtagc tacggagttg
    11401 ctgttcataa aggaattggt acaatcacta aagacaagaa cgtcttggta aatggttctg
    11461 aattgcttga aaccaagaaa atcattcttg ctggtggttc aaaagtcagc aagatcaacg
    11521 tccctggtat ggaatctcca cttgtcatga ctagtgatga cattcttgaa atgaacgaag
    11581 tgccagaaag tcttgttatc atcggtggtg gagttgtcgg tatcgaactc ggtcaggcct
    11641 tcatgacatt tggttcaaaa gtgactgtta tcgaaatgat ggaccgtata gttccagcta
    11701 tggatgtgga agtttctaag aaccttcgct tgatccttga gcgtaaagga atgaccatct
    11761 tgactggtac taaactgcaa gaaatcattg aggaaaatgg tcaacttcgt atcaaggttg
    11821 aaggaaaaga cgatatcatc gcaagcaaag ctcttctttc aattggtcgt atgccagacc
    11881 ttgaaggtat tggagaggtt gagtttgaat tggatcgtgg ttgtatcaag gtcaacgaat
    11941 acatggaaac ttcagttcca ggcatttatg caccaggtga catcaacggt actaagatgt
    12001 tggctcacgc agctttccgc atgggtgaag tttccgctga aaatgccctt aaaggaaatc
    12061 atgcagttgc caaattgaat ttgactcctg cagccatcta cactcttcct gaagtagcag
    12121 cagtaggttt gacagaagaa caagcccgtg agaaatacga tgttgccatc ggtaagttta
    12181 actttgctgc taacggtcgt gctattgcat ctgacgcagc tcaaggtttc gtaaaagtta
    12241 tcgctgataa gaaatacgga gaaatccttg gtgtacacat tattggtcct gcagccgcag
    12301 aattaataaa cgaagcatca agcatcatcg aaatggaaat cactgttgag gaaatgctga
    12361 agaccatcca cgggcaccca acttactctg aagtgatgta cgaagcgttt gcagatgttc
    12421 taggaatggc catccattca cctaagaaaa aataaaacga agatagatta gactggaagg
    12481 ttatttccag tttctatcat ataaagtaaa gggatatcat gaaatacatt atcaatcatt
    12541 caaatgacac tgcttttaat attgccttgg aagaatacgc ctttaaacac cttttggatg
    12601 aggatcaaat cttcctactt tggattaaca agccatctat cattgttggt cgtcaccaga
    12661 acactatcga agaaatcaac cgtgattatg ttcgcgaaaa tggtattgag gtagttcgcc
    12721 gtattagtgg tggtggagct gtttaccacg atttaaataa cctcaactac acgatcatct
    12781 ccaaagaaga tgaaaataag gcatttgact tcaagagctt ctcaactccg gttatcaata
    12841 ccctggctca actcggggtt aaagctgagt tcacaggtcg taatgacctt gagattgatg
    12901 gtaagaagtt ctgtggcaat gcccaagcct atatcaacgg ccgtatcatg caccacggtt
    12961 gcttgctctt tgacgttgat ttgtcagtcc tcgcaaatgc ccttaaggtt tcaaaagata
    13021 aatttgaatc aaaaggtgtg aaatccgtcc gtgcccgtgt aaccaatatt atcaatgaat
    13081 taccaaaaaa aatcacagtc gaaaaattcc gtgatttact cttggaatac atgaaaaaag
    13141 agtacccaga gatgactgaa tacgtctttt cagaagaaga attggccgaa ataaatcgca
    13201 tcaaggatac taagtttgga acttgggact ggaactatgg taaatcacct gaatttaacg
    13261 tccgtcgtgg aaccaaattc actagtggta aggttgaagt ctttgctaac gttactgaat
    13321 caaaaatcca agacatcaag atttatggtg acttctttgg tattgaagac gttgctgcag
    13381 tagaagctgt ccttcgtggg gtgaaatacg aacgtgaaga tgttcttaag gcgctaaaaa
    13441 ccattgatat cacacgctac ttcgctggta ttagccgtga agaaatcgct gaagcggtag
    13501 ttggataaat caaaaagaag ttggccaact aaccaacttc tttttttgat atttatttac
    13561 ataatttaaa atacgtaaaa tcataaacta tccagagcat tcttttgttc atcactaaca
    13621 atatgggtat agaggtcagt gacttgtgtg ctagcatgtc ctagttggtg actgactaaa
    13681 acttgtgatt tagtcgcatc atagagccta gttgctagtg tatggcgcag tttatggggt
    13741 gttacacgca ctttaaaatc ctctgagtat ttagcaacca ttttctcaac gctagaagca
    13801 tcgatacgat taggaacacc tctgtagaga gttaaaaaaa gggctgtatc tgttttttcc
    13861 gttttatagc gttgattccg aatgaccaga taattctcta aataaggttt agcaaaagca
    13921 gcgacattga ctgagtcacg tttgccacct tttcgagtaa catcaataac catcattttt
    13981 agattgagat ctcttagatc tagattaaca gcttcagata agcgaacacc agatgccaag
    14041 agaagggcaa taatggctaa atctcgttct ttatttttgt tgaatgatga gagagctcga
    14101 tttgaaagct gttgtgggtg ctcttgatcg atataagtta gaaaaccttc tgtttcatca
    14161 cctagaaaga gtttttgctt gatattttca gctctggcag caagggtttc tttctttttc
    14221 ttggttgaaa cttttttcat tacattacga tagaaataag gttccccctg atcgttttca
    14281 acctcctcgg ttagatactt gtaaagacta gaaagtgctg ataaggttcg attgatagtt
    14341 gtctgtgaaa caccttgttt tgttgtatta gcattcagca agggacgttc acgtagataa
    14401 aggataaagg attccatgtc tttcttagac atattttcca aaactgataa aggaatatcg
    14461 gatattttat cagcgtttga aataccagac tccaaaaccc agctgaaaaa tcggtcatat
    14521 tccttaaggt attcgtacag ggttgtaaaa ctgtagggca cagccagttt agattggtag
    14581 tattccagaa cataccaggg catgagttgt tttagtttgt cgattcgttc cagtaaaatc
    14641 tcacgtttca tgtatttcct ccataattaa gtatactagt agtatagcat agacttgtat
    14701 atttttcaag aaaattatag tttttctgaa agatgttata ggagtttttc aaagtgataa
    14761 aaagacttag acctacaaac aaaacagaat aaggaaaata ataaaaatct cacccactaa
    14821 acttctgtta atactacata actgaagagc tatataagga gctatgagat acaaaacaca
    14881 ttgtataata gattgaaact agaatagtac atctgaactt ctaaaacatt gctagaaatt
    14941 gatttgattg tcctgattgt tttatcctat ttctatttca tttcactata taattgctat
    15001 tgttttcttt ttcatgattt taatcttact gtacagattt gatacagtag
//
LOCUS       DBERMR010000047        14799 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.047, whole genome shotgun sequence.
ACCESSION   DBERMR010000047 DBERMR010000000
VERSION     DBERMR010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 14799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14799
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.047"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            173..1936
                     /gene="aspS"
                     /locus_tag="ACL8A1_001669"
     CDS             173..1936
                     /gene="aspS"
                     /locus_tag="ACL8A1_001669"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HGS1202407.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEALAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSAEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
     gene            1914..2855
                     /locus_tag="ACL8A1_001670"
     CDS             1914..2855
                     /locus_tag="ACL8A1_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HGS1202408.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(3146..4132)
                     /locus_tag="ACL8A1_001671"
     CDS             complement(3146..4132)
                     /locus_tag="ACL8A1_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGS1202409.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            complement(4142..4942)
                     /locus_tag="ACL8A1_001672"
     CDS             complement(4142..4942)
                     /locus_tag="ACL8A1_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HGS1202410.1"
                     /translation="MLPYPFSFFSSILGFRKPLSKRFGLNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTVSQASSVVIG
                     PSQIKELPKNLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            complement(5195..6037)
                     /locus_tag="ACL8A1_001673"
     CDS             complement(5195..6037)
                     /locus_tag="ACL8A1_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HGS1202411.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(6039..7331)
                     /locus_tag="ACL8A1_001674"
     CDS             complement(6039..7331)
                     /locus_tag="ACL8A1_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HGS1202412.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            complement(7442..8713)
                     /locus_tag="ACL8A1_001675"
     CDS             complement(7442..8713)
                     /locus_tag="ACL8A1_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGS1202413.1"
                     /translation="MSSKFMKSTAVLGTVTLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIVGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            9249..10781
                     /gene="malQ"
                     /locus_tag="ACL8A1_001676"
                     /pseudo
     CDS             9249..10781
                     /gene="malQ"
                     /locus_tag="ACL8A1_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
     gene            10807..13065
                     /locus_tag="ACL8A1_001677"
     CDS             10807..13065
                     /locus_tag="ACL8A1_001677"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HGS1202414.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIDLDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(14109..14681)
                     /locus_tag="ACL8A1_001678"
     CDS             complement(14109..14681)
                     /locus_tag="ACL8A1_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202415.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALA
                     FAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
BASE COUNT         4426 a         2801 c         3170 g         4402 t
ORIGIN      
        1 tactacggta aggcgacgct gacgtggttt gaagagattt tcgaagagca ttagcgctaa
       61 tttttagggc tttcacctga cttttaaaga ctggcaaact tttctgatga ttttcatgaa
      121 gagcctgcgc ttttatggta aaatagtaac agaataaaag aggagagaaa taatgaaacg
      181 tagtatgtat gctggtcgtg ttcgtgagga acacatcgga caagaaataa ccttgaaagg
      241 atgggttggc cgtcgtcgtg accttggtgg tttgatcttt atcgatcttc gtgaccgtga
      301 aggaatcatg cagttggtta tcaaccctga aaaagtatct gcagaggtta tggcaacagc
      361 tgaaagcctt cgtagcgaat ttgttattga ggtgactggt caggtcgctg cgcgtgagca
      421 agccaatgat aagttgccaa ctggtgcggt tgagttaaac gtgacagctc ttattgtgct
      481 taacacagct aagacaacac catttgagat taaggatggc attgaggcaa atgacgatac
      541 acgtttgcgt taccgttacc ttgaccttcg tcgtccagaa atgttggaaa atcttaaact
      601 tcgtgccaag gtgacccact ctatccgcaa ctacttggat gagttggagt ttatcgacgt
      661 ggagacacca ttcctttcta agtcaacgcc tgaaggggcg cgtgattatt tagtgccgtc
      721 tcgtgttaat aaggggcatt tttacgctct tcctcaaagt ccacaaatca cgaaacagct
      781 cttgatgaat gctggttttg accgttacta ccaaatcgtt aaatgtttcc gtgacgagga
      841 cttgcgtgga gaccgccagc ctgaatttac tcaggtcgac ttggaaacgt ccttccttac
      901 tgagcaagaa atccaagata ttacagaagg cttgatcgcg cgcgtgatga aggaaacaaa
      961 aggcatcgaa gtaacgctac cgttccctcg tatgaaatac gatgatgcta tggctcttta
     1021 cggttctgac aagccagata ctcgttttga catgttgctt caggacttga cagaagtggt
     1081 caaaggtgta gactttaaag tcttttcaga agcacttgct gtaaaagcga ttgtggtcaa
     1141 aggagctgcg gacaactatt cacgtaaaga catcgacaag atgacggaag tagccaaaca
     1201 gtatggtgcc aaaggtcttg cttgggtcaa ggtggttgat ggagaattaa acggaccagt
     1261 tgccaagttc ttgactggta tccaagaaga attgacaaca gcgcttgctc ttgaagataa
     1321 ggacttggtt ctctttgtgg cggatacgct tgaagtggct aatgcaacac tgggtgccct
     1381 tcgtggacgt attgctaaag agcttggctt gattgataat gataagttca acttcctttg
     1441 ggtggttgac tggccgatgt ttgaatggtc tgaagaagaa ggccgctaca tgagcgccca
     1501 ccatccattc acacttccac aggaagagac tgctcacgaa ttagaaggtg atttggctaa
     1561 ggttcgtgcc attgcttacg atatcgtctt gaacggttat gagcttggtg gtggtagcct
     1621 tcgtatcaac caaaaagacc ttcaagaacg catgttcaag gctcttggtt tctcagctga
     1681 agaagccaat gaccagtttg gtttccttct tgaagccatg gactatggtt tcccaccaca
     1741 cggtggtttg gctatcgggc ttgaccgttt tgtcatgttg cttgctggag aagaaaatat
     1801 ccgtgaagtc attgcctttc ctaagaacaa caaggcaact gacccaatga cacaagctcc
     1861 atcaacagtc gctctcaaac aactagagga actcagctta caagtagaag aagatgaaac
     1921 aaacaaaacg aattaagcgg tggcgctatt atctgcgccg ctttgcttat cagataaaaa
     1981 ttttacgtgt cttacaaagt atctctcgag aaaagtatga tgagaagatt tcggcctctc
     2041 tggtctatgg ttttttatca gcagtagcag ttaatttctt tttccaacca gggcatgtgt
     2101 attcgagtgg tgcaacaggt ctggcacaga ttatctctgc cttgagtaat cactggtttg
     2161 gttttcatat tccgatttcg ctaagcttct acgccattaa cttccctttg atggtcttag
     2221 cttggtatca gattggccat aagttcaccg tctttacctt tatcacggta tctatgagtt
     2281 ccttctttat ccagtttgtc cctgtggcaa ccttgacgga ggatcccatt atcaattccc
     2341 tttttggggg tgttgttatg ggtttgggga ttggttttgc tcttcgaaac aatatctcca
     2401 gtggtgggac ggatatcgtc agcctgacta ttcgcaagaa aacgggtaag aatgtcggta
     2461 gtatttcttt cttggtaaat ggaactatca tgctgatagc aggtttgacc tttggttgga
     2521 aatacgctct ttattctatg attaccatct ttgtctctag ccgtgtgaca gacgcagtct
     2581 ttactaagca aaagcgtatg caggccatga ttgtgacaaa tcatccagag aaggtaattg
     2641 aaaaaatcca taaaaaattg caccgcggag caaccatgat ccacgatgca gaaggaacct
     2701 ataatcacga gagaaaggca gttttaatca ctgtcattac acgtgcagag tttaatgaat
     2761 ttaaacagat tatgacacaa gtggatccaa gctcctttgt ctctgtctcg gaaaatgttc
     2821 atattctagg aagatttgtt gagatagata attagtgatt aatactcttc gaaaatctct
     2881 tcaaaccacg tcagcgtcgc cttgccgtag gtatgttact gacttcgtca gttctatcca
     2941 caacctcaaa acagtgtttt gagcaacctg cggctagctt cctagtttgc actttgattt
     3001 ttattgagta taaaaagatc ttgggtaaca actcaatttc agaagaaaag gaagtaaatc
     3061 ttcccacaat aaaacgcata ctatcaagtt ttttcaatac ctgatagtat gcgtttttgc
     3121 ttttaacgac tttttgccca gtctcttatt tttcgataat tttgtgggcg atcaattgac
     3181 ggtaacaaat ttgtttattg tttttattat cattaataat ctggagaata gtttcaaggg
     3241 aaacacgacc aagctctaaa ctattgatat cgacataggc tgccaagttg agcttgggat
     3301 taaccgagtc aaagctgaga acagggacat ccagctggtg tttggcaata tagttacaaa
     3361 caccttcagc taggaggcta tcggttgtga tgatagcatc aatttgtgga tcgtgcttga
     3421 ataatcgctt gctaaattta tagccctttt cttccagaaa ctcgtcggca aagtagatgc
     3481 gattgttgtc agtggtaagt ttgtaatgtt taagcgcctg ttcatagccc gttaaacggt
     3541 ctttggtcac gaagagcttt ttacttcctc cgataaaggc aatgcgtttg cagccttttt
     3601 tgatgaaata ttcagtcgca tcaaaaccag cttgaacatt gtcgttgtcg acaagtggga
     3661 tgaaaggaga tagagattta cctaagataa ggaaggggaa ctgttcttct gcgacgagtt
     3721 ttacgagagg gtcttcttct tgggcataga gaaagattag cccatctaca cgcttgccgt
     3781 agaccatttg tgaaatagcg ttgagacgct ccttctcatc tttccctgtt gctatctgaa
     3841 tggcatagtg gttttcagat gcgacttgag agatgccacg tagaaccgat ggaaagaaag
     3901 gattctggta gaaggcgtct gagtcatcag gaagaactaa tccgataacc tgagtatagc
     3961 tgcttaccaa gctacgagcg ttgaggtttg ggtggtagtt gagttccttc atagctttgc
     4021 gaacgcgttt ttttgtttcg tcgctaatgg ttgatttatt ttgaataaca cgggttacgg
     4081 ttgaaggcga aacaccagca gccttggcca cgtctttaat cgtaacgggc ataaaaatct
     4141 cctatttatg gtaatttgga tcacggaaat gtgttttata aaagatagtg accagataca
     4201 gaacaaaaag aatattttgt acagtaatca gggttgtcat attttggcca aataatccca
     4261 aaataagtgt aatcagagtc ggcaatccta aacagttcaa gataaagtga tagcactctt
     4321 taaaggttct aaatgaaaag aggcgtgatt tcttagtgat ataaaggaga agactagctc
     4381 ctagagagac gataaagaaa ttcaaaccaa agaggaagct cgcaccgaga actaggaaga
     4441 ggctgatata gacacgattt tgttggtacc agtctttaga aattgcttgg gtcaagctgt
     4501 ctttgctttt gaaactctca gtctgaatgg ctcggtaaga gatgcgggtc agttccttgc
     4561 tttccttgct gatgactagc tcatttgtat cgaaatgcag ttgcaagttc ttaggtaatt
     4621 ccttgatttg acttggacca atcacaacag aagaggcttg actaactgtt ccagtataag
     4681 ttaatgtgcc atcgacaatt gtagcatgtt cagaaagatc ctggacaacc tcatttgtca
     4741 gaggttcata gacattatcg ataaaagttt ctagcggata ggtctcctgg gagctgtttt
     4801 ggatagcaat gggtaccata gacaagctga taaggaagat actggtaaag agaagttgaa
     4861 accagttgag cccgaaacgt ttggacaggg gcttacgaaa ccccaaaata cttgaaaaaa
     4921 aggaaaatgg atatggaagc ataggtatct ttctaaaagg tgttttctat ataagtatta
     4981 ttatatagag aaatgcgctt tatttcaagt ctaggagaaa aattgcttgc tacaaggata
     5041 ttatttacag tgacaattgc taatagtcaa tgaaaatcaa agtgcaaact agccacaggt
     5101 tgctcaaagc acaactttga ggttggagat aaagctgacg tggtttgaag agattttcga
     5161 agagtataaa atgaaaaagg gtggcgggga taaattatcc cttgtcgcca ccacttgtaa
     5221 gtcctgaaac aaagttcttt tgtaggaaga agaagagaat acagattgga agggcgatga
     5281 ggatagcacc tgctgagaag taggcaatct tcaagttttt cgcattgtta acgaaggttt
     5341 ggagacctac ggcaacagta aagtattctt tctcacgaag caagaaacta gagaggatgt
     5401 agtccccgaa aggtcccatg aaggcccaga gagcttgtac ggcaaccatt gggcgaacaa
     5461 gtggaagaac aatttgccag aagcggcgga agtgtcctgc accgtctagt tttgcagatt
     5521 cgtctaaaga cattggcact gtatcgaagt agcctttcat gagccaagca ttcatcggga
     5581 taccaccacc aacgtagagg aagatgagga accagttgtg gttaagggcg ttcaacataa
     5641 gcgccataac gaagaaggct gtcaaagcgg ccatagttgg caccatttgg atgatcaaga
     5701 agaagaccaa actttgttta cgagccaaga agttgtaacg gctgtaagca taaccagcaa
     5761 gtacgatgat acttgtttga acagccatgg taattaaggc gataatcaaa gtgttgaggt
     5821 accaagtacc gtacaaggtt tcagtgaaga ggcctttaaa gttatcaaaa ttgaggtcga
     5881 tattagtatc tagtttaaag gctgagacat tacctgcttt aaaggctgac ataatggtaa
     5941 tcaacagtgg atagataatt acaattgata gaccaatcag gtaaaggtaa gtaaggcttt
     6001 gagtcagtct acgtttgagt ttaattgagt tattcatctt agacgtcctc catatcaaat
     6061 gcgtgtagtt tcttgaatgc gatcatagag attgagatga caatgataga gataatcaag
     6121 gtaacagctg ccgccattga gtattgagga gatgtacctg ttgtcaaacg gtagatccat
     6181 gagatcaaga tatcggttga accagctcca cctccgacac taccaggtcc tccaccattg
     6241 aagaggtaca tgatagagaa gttgttaaag ttgaaggtgt attggctaat caaagtaggt
     6301 gccgcaacag ccaaaatcat tgggaaagtg atgttgcgga atttttgcca agcgttggca
     6361 ccgtcaatat aagctgcttc gtaaaggtcg ttaggaatag attgcaagat acccaaagtc
     6421 aaaacgtaga tgtatgggaa tccgagccaa ccttgcatca taatcaaggc aatcttagtc
     6481 caagttgggt ctgttttcca aggaataaga gctccatcaa ggaaaggaag gaatttagcc
     6541 aagattggca atacttgagt gttgatagca ccgacactat cgttaaacat gtttgagaat
     6601 gtcaagatag tgatgaaggc tgggacagcc caaggaagaa ggaaaataac accaaagata
     6661 cgttttcctt tgataaatgg ttggttggca atgatagctg tgaagatacc aattacgatt
     6721 tgtaaagtag aagctgccaa agcccaaatg atagtccaag aaagaacaga accaaaggca
     6781 gaacggaagg tactcaagct ccaaatgttt gtaaagttgg tcaaaccaac ccagtccaac
     6841 aacttgtttg gtggcaagtg ttggaagtcg tagttggtaa aggcgatcat caaggttacg
     6901 ataactggga agataatcgc gaaggtcatg gcaacataag atggaatgat caagaggtaa
     6961 gggaagccat tttcataaat ccctttgatc atgtctttga gtgtgcgtgg aactggaatt
     7021 ccattgttaa tgcgttttgc aatcgtatgt gcatctttga tatttgagaa ataaaagagt
     7081 acataaacga ttacaaagat tagatggaag gcaccacgaa tcagcataaa gagggaatta
     7141 tcacgacctg gtttgtcacc aagagtgatg aggttgctca attcaggggt tgcaagtgct
     7201 aggaagtaaa ggacaaatac gatggttaca ccaaggaaga taaaaccttt ggctttttgt
     7261 ttattgtaaa tttgtcctaa cccaggaatg atagacagca gggctgcttt actaggttgt
     7321 tgcttttcca tattaactcc tttcatagga tactactttt ttataagaaa gctaaattat
     7381 ttgtgtctct attgataaat tcaaaggggg atttgatttc cacccccctt gaacaaattt
     7441 tttattcacc aaatttttgt ttgattgttt ctttgatcaa tgttacagca tcgttagcag
     7501 ctgttttagc atctttttga ccacttacag catcaaagag catatttttc gctggatccc
     7561 aaactgcaga catttgagag atgtttggca gtggttgagt gttcttgaac tgtttgataa
     7621 cagctgttgt caactcatcg tttttacctt cagcgtatga acgagcctca gtattagctg
     7681 ggatttcgtt agtcttatca tataatactt tttgttgttc agttgcaaca aggaagtcta
     7741 caaatttttg agaagcttca aggttcttaa cggcttgagg aatgacccaa gctttaccac
     7801 caccgaatgc agcatattct tttccatttg gaagagttgg gatagttgca actccgtagt
     7861 ttactttagc atctttaaag gcttgagctt tccaaggtcc gtcgatgata gcagctgttt
     7921 taccttcttg gaattgagtt tggattaagt ttccagcacc ttctgtatct tgcatacctt
     7981 taggccattt ttcgtaccaa gatttagcgt aattgatacc tacgatagaa ccgtcgtttg
     8041 caagaccgat gtctttagcg tctttaccgt tttggccaaa gacgtaagca ccgttaccgg
     8101 caagaagtcc atatgtatag tagaagtttg tccagtcagc taggaaggca gtagttttac
     8161 catcttcacc agcgaatgcg tatttgctat ctttagcaag gttttccaag tcagcaaatg
     8221 tttttggagc atctttcacc aagtctttgt tgtagtacat aacaagtgac tcgataacgg
     8281 caggagcacc gtaaacttta ccattagcag ctgttacaag agatttagtt gtgtcgtctg
     8341 ttttagcacc atcgctcaat ttcacttctg aaagttgtcc gtcagaacca aggctaccta
     8401 cacggtcgta tggagccatc ataacatcag ggacattacc agattggttg tcaagagaaa
     8461 gtttatcaag acctcctaga gcatcaccag ttttaagagt gacttttact ccagcttctt
     8521 tttcataagc tttagcaacc tcttcaatat agctcttata tccctcgtct acatatacag
     8581 tgagttcttt gacttcagat gaaccagaat cagcaggctt atcagcagtt ttgcttccgc
     8641 aagctaccaa aagcaagcta gcaagtgtaa cagttccaag caccgcagtg ctcttcataa
     8701 atttagatga catagtgtat tcctcccaaa gaatagcaag ttttattgat aaggaaacgc
     8761 aaacgttttc ctttatgagc ttagtatagc acaaatagaa aacggttgca agtatttttt
     8821 gtaaaaattt taaaaaattt ttaagcttga tttgatagca taattttgca ttttaataga
     8881 agaaaagtat gaaatagata agaatacagt atttttaata aaaaatatat ggaatctgtc
     8941 taaagaggga aatcaggagg ggctacctcc ctgtttctaa tccctatcta ttttctatag
     9001 acgtttgtgc tttgctgcat ataaattcaa gctccctaag gaggttttgt actttgtaga
     9061 ctgtctcgtg agagtcaaac tttaggatta gatagtttta agttatcttt gctaaaatga
     9121 gttagtctcc ttaaaaataa tcaaaaaagt tttttaaaaa cgcttgcaat tatgcttgaa
     9181 aaggagtata cttataagta acgcaaacgt ttgcgtctgc aaaatacgca acgttccatt
     9241 attttaacac acgaggtgct attatgaaaa aacgtcaaag tggtgtgttg atgcacatct
     9301 cttctcttcc aggagcttac ggaatcggat catttggtca aagtgcttac gacttcgttg
     9361 atttcttggt ccgtacaaaa caacgttact ggcaaatcct tccattagga gcaactagtt
     9421 acggggattc tccttaccaa tctttctcag ccttcgcagg aaacactcat tttatcgatt
     9481 tagatatctt ggtggagcaa ggtttgttgg aagcaagtga ccttgaagga gttgactttg
     9541 gtagcgatgc gtctgaagtt gactatgcta aaatctacta tgcacgtcgt cctcttttag
     9601 aaaaagcggt gaaacgtttc tttgaagtcg gagatgttaa agattttgag aaatttgctc
     9661 aagacaacca atcatggctt gagctctttg ctgagtatat ggctatcaaa gagtattttg
     9721 acaatcttgc ttggactgaa tggccagatg cagatgctcg tgctcgtaaa gcttcagcac
     9781 ttgaaagcta tcgtgagcaa ttggcagaca agttggttta ccaccgtgtg actcaatact
     9841 tcttcttcca acaatggttg aaattgaaag cttacgctaa cgacaaccac atcgaaatcg
     9901 ttggggacat gccaatctac gtagcggaag attcaagtga tatgtgggca aatccacatc
     9961 tcttcaaaac agatgtcaat ggtaaggcta cttgtatcgc aggatgccca ccagatgagt
    10021 tttctgtaac tggtcagctt tggggtaacc caatctatga ctgggaagca atggacaaag
    10081 acggttacaa atggtggatt gaacgcttgc gtgaaagctt caaaatctac gatatcgttc
    10141 gtatcgacca cttccgtggc ttcgaatctt actggcaaat ccctgctggt tccgatacag
    10201 cagcacctgg tgagtgggtg aaaggtccag gctacaagct ttttgcagcc gttaaggaag
    10261 aacttggtga gctaaacatc atcgcagaag accttggctt catgacagat gaagtgatcg
    10321 aattgcgtga acgtactggc ttcccaggaa tgaagattct tcaatttgcc ttcaacccag
    10381 aagacgaaag cattgatagc ccacacttgg cacctgctaa ctcagttatg tacacaggaa
    10441 cacacgataa caatacggtt cttggttggt accgtaatga gattgatgat gcgactcgtg
    10501 agtacatggc tcgttacacg aaccgtaaag aatacgaaac agtggtacac gctatgcttc
    10561 gtacagtatt ttcatcagtt agctttatgg caattgcaac tatgcaagat ttactagaat
    10621 tggatgaggc agctcgtatg aacttcccat ctacccttgg tggaaactgg tcttggcgta
    10681 tgactgaaga tcaattgaca ccagctgtcg aggaaggttt gcttgacttg acaacaattt
    10741 atcgccgaat taatgaaaat ttggtagatt taaagaaata agacaataat caggagacaa
    10801 ctaaacatgt tatcactaca agaatttgta caaaatcgtt acaataaaac cattgcagaa
    10861 tgtagcaatg aagagcttta ccttgctctt cttaactaca gcaagcttgc aagcagccaa
    10921 aaaccagtca acactggtaa gaaaaaagtt tactacatct cagctgagtt cttgattggt
    10981 aaactcttgt caaacaactt gattaacctt ggtctttacg acgatgttaa aaaagaactt
    11041 gcagctgcag gtaaagactt gatcgaagtt gaagaagttg aattggaacc atctcttggt
    11101 aatggtggtt tgggacgttt ggctgcctgc tttatcgact caattgctac tcttggtttg
    11161 aatggtgacg gtgttggtct taactaccac tttggtcttt tccaacaagt tcttaaaaac
    11221 aaccaacaag aaacaattcc aaatgcatgg ttgacagagc aaaactggtt ggttcgctca
    11281 agccgtagct accaagtacc atttgcagac tttactttga catcaactct ttacgatatt
    11341 gatgttactg gttatgaaac agcgactaaa aaccgcttgc gtttgtttga cttggattca
    11401 gttgattctt ctattattaa agatggtatc aactttgaca agacagatat cgctcgcaac
    11461 ttaactctct tcctttaccc agatgatagt gaccgtcaag gtgaattgct ccgtatcttc
    11521 caacaatact tcatggtttc aaacggtgcg caattgatca tcgacgaagc aatcgaaaaa
    11581 ggaagcaact tgcatgacct tgctgactac gcagttgtcc aaatcaacga tactcaccca
    11641 tcaatggtga ttcctgaatt gattcgtctt ttgactgcac gtggtatcga tcttgacgaa
    11701 gcaatctcaa ttgttcgtag catgactgcc tacactaacc acacaatcct tgctgaagcg
    11761 cttgaaaaat ggcctcttga attcttgcaa gaagtggttc ctcacttggt accaatcatc
    11821 gaagaattgg accgtcgtgt gaaggcagag tacaaagatc cagctgttca aatcatcgat
    11881 gagagcggac gtgttcacat ggctcacatg gatatccact acggatacag tgttaacggg
    11941 gttgcagcac tccatactga aatcttgaaa aattctgagt tgaaagcctt ctacgacctt
    12001 tacccagaaa agttcaacaa caaaacaaac ggtatcactt tccgtcgttg gcttatgcat
    12061 gctaacccaa gattgtctca ctacttggat gagattcttg gagatggttg gcaccatgaa
    12121 gcagatgagc ttgaaaaact gttgtcttat gaagacaaag cagctgtcaa agaaaaattg
    12181 gaaagcatca aggctcacaa caaacgtaaa ttggctcgtc acttgaaaga acaccaaggt
    12241 gtggaaatca atccaaattc tatctttgat atccaaatca aacgtcttca cgagtacaaa
    12301 cgccaacaaa tgaacgcttt gtacgtgatc cacaaatacc ttgacatcaa agctggtaac
    12361 atccctgctc gtccaatcac aatcttcttt ggtggtaaag cagctccagc ctacacaatc
    12421 gctcaagata tcattcactt gatcctttgc atgtcagaag ttattgctaa cgatccagca
    12481 gtagctccac acttgcaagt agttatggtt gaaaactaca acgttactgc agcaagcttc
    12541 cttatcccag catgtgatat ctcagaacaa atctcacttg cttctaaaga agcttcaggt
    12601 actggtaaca tgaaattcat gttgaacgga gctttgacac ttggtactat ggacggtgct
    12661 aacgtggaaa tcgctgagtt ggttggagaa gaaaacatct acatcttcgg tgaagattca
    12721 gaaactgtta tcgaccttta cgcaaaagca gcttacaaat caagcgaatt ctacgctcgt
    12781 gaagctatca aaccattggt tgacttcatc gttagtgatg cagttcttgc agctggaaac
    12841 aaagagcgct tggaacgtct ttacaatgaa ttgatcaaca aagactggtt catgactctt
    12901 cttgacttgg aagactacat caaagtcaaa gagcaaatgc ttgctgacta cgaagaccgt
    12961 gacgcatggt tggataaagt catcgttaac atttctaaag caggattctt ctcatctgac
    13021 cgtacaatcg ctcagtataa cgaagacatc tggcacttga actaatactc ttcgaaaatc
    13081 tcttcaaacc acgtcagctt tatctgcaac ctcaaagcgg tgctttgagc aacctgcggc
    13141 tagcttccta gtttgctctt tgattttcat tgagtataag atacaaattt atactaatac
    13201 atcttgtaaa aaagcgagtt tcgattgaaa ttcgcttttt taatgatgta gatttgggtc
    13261 aatcttgtct aaaaataggg aaatcctaga tacagtgaag gctttaaatg ctggttttta
    13321 ctgtcttcag ccttatattt tttcgtagtt ggttacctca tatctcttgt attcgcttac
    13381 ataaagtatt ataatataat tgtaggaaag aaggtgtttt tatgatatac acacttaaat
    13441 tggtgttgtt tattaccttt cttgtaataa gcttgttacc tgataagatt tttggaaaaa
    13501 ataaaaaaat ttggaaaata gtttttgcaa tattgacggc agtggcagca ttgtcattta
    13561 tgtactaagt tattttaaga atgtagggaa ataaacccta cattcttttt agttttttct
    13621 gttttctaaa ttctatttat ccaagcgctt caacatttct tgcttcttcg cttcaagttc
    13681 tgcacgcttt tcttcgattt cggcatgttt tttctcgagt tcagaacaac ttgcatcatt
    13741 gctaaattct tttcgccatc aggagatagg gtgagtcgac atgtctatta ctcacccaaa
    13801 gcagtcctac aaagcaggaa ttttctgtta cttttttgga aatagtaacg tttatacagc
    13861 tttgacactt cgtatcaaag cgccaaacac actccgaggg atttacagaa agcagaaaag
    13921 gaatgatctg gtataagatc attccttttc tctctttttc tttaagtaat tatatacaat
    13981 gtacgacgaa gtcgtcattg caatgctgat ccaccaccta aagggaactt taaacaacat
    14041 tgataagata aagaatataa acaacgaaaa tacgttatac ccaattaatt ttattgtata
    14101 tctcatgatt aaaagttaat ccttccgttg ttaggaatgg catcattttt atcccataat
    14161 tgtgctaaat aagtccccgg tgataataaa ttcatagcga atgctaaagc aacatcattt
    14221 acaaaccaac tacctagata tctagaaatt gctgaacgaa tagcactttt tgctgcatgt
    14281 tttcctttta ctttaattag atttgcaagg cctgcagtag ttcctcctaa tgctaaagct
    14341 attgcagtat ctaatagagc acccatttga ttaactgtaa taccttgcca agctgctcta
    14401 aatggagagt atgtaggtgg gattgtataa tcgccttgta attgtcggtt aattacttct
    14461 ttgatccatt gttgtgagac gtctggatga aaagattgga tttcgtttgc aagtgtattg
    14521 atttgttctt ctgttagaga agtgacaggt tgaagttcca tatttgtttc aatttgtgat
    14581 acttgttcag aagcgtatac agctgaaaca cttggaatcg ctgatacaat taacacaatt
    14641 gacgtcaaaa aaaccgaaat aaatttcatt aatttgttca tgagcttttc tcctttttat
    14701 ttgtatctgc ttacatttta ttatatactt ttattatagt caagaaaaat atgctattat
    14761 gttaaaaaaa tatttttcaa aatataaatg gacggattt
//
LOCUS       DBERMR010000048        14700 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.048, whole genome shotgun sequence.
ACCESSION   DBERMR010000048 DBERMR010000000
VERSION     DBERMR010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 14700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14700
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.048"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(7..1362)
                     /gene="rlmD"
                     /locus_tag="ACL8A1_001679"
     CDS             complement(7..1362)
                     /gene="rlmD"
                     /locus_tag="ACL8A1_001679"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HGS1202416.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIAKRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
     gene            1400..2176
                     /gene="recX"
                     /locus_tag="ACL8A1_001680"
     CDS             1400..2176
                     /gene="recX"
                     /locus_tag="ACL8A1_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HGS1202417.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINTNQLSGDKGPYVLTQKLAQKGISKSTIEENLKEFDFSEVAQRVANK
                     LLKKYEGKLPSRALQDKIIQNLTNKGFSYSDAKIAFDDLDSQVDQETTQELIFKELDK
                     QYTKYARKYEGYELKQRLSQVLARKGYDFSDIASALREYL"
     gene            2265..2798
                     /locus_tag="ACL8A1_001681"
     CDS             2265..2798
                     /locus_tag="ACL8A1_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HGS1202418.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(3152..3661)
                     /locus_tag="ACL8A1_001682"
     CDS             complement(3152..3661)
                     /locus_tag="ACL8A1_001682"
                     /inference="COORDINATES: protein motif:HMM:TIGR01716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202419.1"
                     /translation="MVQGLLNQLDCNHVVSRDDLNLVYDYLFQKERWESYEITLIGNL
                     YHLFEIDYIYMVGKEILERTHYYEKIGKNRNLVVSACLNFWFCCLENSHLIYADYFEM
                     KLQKLLKDDTKVFEKSTFKFVEGYKIYLTESKESGIKQMDNVIKYFEFIESKSIALYF
                     QKRLNELID"
     gene            complement(3670..4008)
                     /locus_tag="ACL8A1_001683"
                     /pseudo
     CDS             complement(3670..4008)
                     /locus_tag="ACL8A1_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(4021..4794)
                     /locus_tag="ACL8A1_001684"
                     /pseudo
     CDS             complement(4021..4794)
                     /locus_tag="ACL8A1_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566051.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(5240..5995)
                     /locus_tag="ACL8A1_001685"
     CDS             complement(5240..5995)
                     /locus_tag="ACL8A1_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HGS1202420.1"
                     /translation="MIDVEEILCKMPPNQKINYDRVMQKMVQAWEKNEQRPTILVHVC
                     CAPCSTYTLEYLTKYADVTIYFANSNIHPKVEYHKRVYVIKKFVSDFNERTGNTVQYL
                     EAPYEPNEYRKLVRGLEEEPEGGDRCKVCFDYRLDKTAQVAMDLGFDYFGSALTISPH
                     KNSQTINSIGIDVQKIYTTHYLPNDFKKNQGYKRSVEMCEEYDIYRQCYCGCVYAAQA
                     QNIDLVQVKKDATAFLLDKDVEKDYSHIKFIVD"
     gene            complement(6133..7755)
                     /gene="groL"
                     /locus_tag="ACL8A1_001686"
     CDS             complement(6133..7755)
                     /gene="groL"
                     /locus_tag="ACL8A1_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HGS1202421.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(7771..8055)
                     /gene="groES"
                     /locus_tag="ACL8A1_001687"
     CDS             complement(7771..8055)
                     /gene="groES"
                     /locus_tag="ACL8A1_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HGS1202422.1"
                     /translation="MLKPLGDRVVLKIEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(8325..8702)
                     /locus_tag="ACL8A1_001688"
     CDS             complement(8325..8702)
                     /locus_tag="ACL8A1_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGS1202423.1"
                     /translation="MIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGEREADFVN
                     MVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLLESRAQR
                     AMRENNAGQDLADLVLEEEELPF"
     gene            complement(8729..8971)
                     /locus_tag="ACL8A1_001689"
     CDS             complement(8729..8971)
                     /locus_tag="ACL8A1_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202424.1"
                     /translation="MKILNIELANVEQTDLGFEHWVDVTYNVPILKNEYTVRLLLLFG
                     FEIEDPEVIEYLVNAWKYRELVLHSVRMYDMEREAS"
     gene            complement(9225..10181)
                     /locus_tag="ACL8A1_001690"
     CDS             complement(9225..10181)
                     /locus_tag="ACL8A1_001690"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HGS1202425.1"
                     /translation="MDIDRNRLRTGLPQVGVQPYRQVHAHSTGNRNSTAQNEADYHYR
                     KDPELGFFSHVVGNGRVMQVGPVNNGSWDVGGGWNTESYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTDDLEGIKTHEYCTNNQPNNHSDHVDPYPYLASWGIS
                     REQFKQDIENGLSAATGWQKNGTGYWYVHSDGSYPKDKFEKINGTWYYFDGSGYMLAD
                     RWKKYTDGNWYWFDNSGEMATGWKKIAEKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFVQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(10181..10516)
                     /locus_tag="ACL8A1_001691"
     CDS             complement(10181..10516)
                     /locus_tag="ACL8A1_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HGS1202426.1"
                     /translation="MQQITEIITNGAISILVVLAGVVVRAVKDYLVQKGGEKTIKIVE
                     ILAKNAVNAVEQVAAETGYKGDEKLAQARAKVRAELTKYNISMTDKDLDTFVESAVKQ
                     MNDAWKGQE"
     gene            complement(10520..10936)
                     /locus_tag="ACL8A1_001692"
     CDS             complement(10520..10936)
                     /locus_tag="ACL8A1_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HGS1202427.1"
                     /translation="MQIEFFNFLRSVVQTEDGLVLYALALIVSMEIIDFVTGTIAAII
                     NPDIEYKSKIGINGLLRKISGVLLLMILIPASVLLPEKTGFAFLYSIYLGYIAFTFQS
                     LIENYRKLKGNVTLFQPIVKVFQRLLEKDDDTKKGE"
     gene            complement(10946..11296)
                     /locus_tag="ACL8A1_001693"
     CDS             complement(10946..11296)
                     /locus_tag="ACL8A1_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202428.1"
                     /translation="MAETIQNTDNLLDLTKITEPFDLASALRYMKENGEFIRCKNVSD
                     DFYMYRDVQKRPVIVNGRRQFKDVETVWAFNQWGGTIATINVAVLLNHEFYIMKFDAE
                     GNPDWTDPTVKPKE"
     gene            complement(11299..11502)
                     /locus_tag="ACL8A1_001694"
     CDS             complement(11299..11502)
                     /locus_tag="ACL8A1_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202429.1"
                     /translation="MNTEQLNQALRMTISEISTTSTNSMITSNILSIQLDEQREENQR
                     LQARVDELEALLDEQTKPADKGE"
     gene            complement(11483..11599)
                     /locus_tag="ACL8A1_001695"
                     /pseudo
     CDS             complement(11483..11599)
                     /locus_tag="ACL8A1_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063632.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
     gene            complement(11596..>14700)
                     /locus_tag="ACL8A1_001696"
     CDS             complement(11596..>14700)
                     /locus_tag="ACL8A1_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HGS1202430.1"
                     /translation="NIRQSVKSLETDTQNKLNQKLSQAEFEVRAGSIRQEILNATKDK
                     ADKTLVVSEAGKLREEFSKMKVGGRNLWIKSKTVGAVIEKLPENHVTGQKECYRLENN
                     STLTFNLEPDFSSRLYQKVTFSAWIKYENVVQGRNFWNVFNCFKHYLFRKNSETGVQS
                     GPDYATLGMYKGSADWKYITFTYDYSEKTNFDQLKTSLRFNLEGATSGTAWVTGIKVE
                     IGSVATDWSPAPEDADGLITEAKATFERTAQGLRTDLSAIQEYVNKDGQRQEALQRYT
                     REESARQATAVRELVNRDFVGKATYQEDVKGINQRIEAVKTSANKDIASQIASYRQSV
                     DGKFTDISSQITTYKQDVGGQISGLSNRLTSSEQGTTTQISNLSNRINSNKQGTDNQI
                     SNLKTQVATNKDNAERQMGRISDQVSANKANADSQFANVTNQLARKVETTDFQRVKET
                     SKLYERILGNTENGIADKVARMALTNQLFQVEVGKYSVSGPNLIKNSDFKNATNEWGS
                     TQNLGRLVKHSFYHNGQKDLMRLSNATKNENFLYSHRFNLERNTDYVLNFRGFNNSAL
                     ASYDVYILGRRAGESDGFTIVKKVVSSKKLSTSRCEDVSVTFNSGEMDNAYIRFDNNG
                     SSSGTADLYITEVDLYKGYKPRTWQPHPEDAVADANKKLEATQTKMTQLAGSWVVENI
                     NSAGDIISGINLGANGHNRLVGKLTHITGETLIDRAVIKSAMVDKLKTANFEAGSVTT
                     TILEAEAVTAEKLKVDNALIKKLTATDAFIDQLISKRIFSTKVESVISSSTFLEAYQG
                     RIGGFTLGQFDQGGGRWISGVNQFSVGMGNGAGYGVRTAFWANWGNNWNYAGPKAWNV
                     NTDGKMYCRNEVGFYDQVDFSNSSRANFYGNTTFSRSPVFSNGIEVGSKDVLGDGWNP
                     KGGRNAVVWWNQVGSGSLKYWMEQKSDRRLKENITDTAVKALDKINRLRMVAFDFIEN
                     KKHEEIGLIAQEAETIVPRIVSRDPENPDGYLHIDYTALVPYLIKAIQELNQKIEKME
                     KTIA"
BASE COUNT         4209 a         3116 c         2524 g         4851 t
ORIGIN      
        1 ccttttttaa acttttttta ttaattttac aacagcttca gttcgagctg tatgtgggaa
       61 catatcgacc gactggatat aatgaagatc atagacttct actaagcgta ccaaatcacg
      121 agccaaggtc gaaacattac aagaaatata aaccattttt tctggtacat aagtaagaat
      181 agtatctaat aacttatcat ccagacctgt acgtggtggg tcaacaatca aagcatctgc
      241 tcggtagcct tccttgtacc aacgaggaat aatctcttct gccgttccag cttcataatg
      301 agtattgtca aatcccattc ttttagcatt tcgcttggca tcttcaatag cttctggaat
      361 aatatccata cctctgagtg tttttacttt ctttgcaaag gcaaatccaa tcgttccaac
      421 tccacaataa gcgtcaatca aatggtcttc tttatcaaca tccagcgctt ttactgcttc
      481 gctatagagg acttctgttt gctcaggatt tagttgataa aaagctctag gggatagtga
      541 aaattcataa ttgagtacac cttcttgaat actctcttgc ccccagataa tctctgtctt
      601 ttcaccatat atctcactgg ttttagctgt atttgtatta acagctactg tcacaacttc
      661 tgggaaatct ttaaccaact cttttaccaa ttgagttaaa ttaagctggc ggtttgtaac
      721 aataataatc tgaacctgtc cggtctttct cgcgcgtcgg accataatag tacggacacc
      781 tagaactttt ctctcatccg tgattggaat ctggtgataa gtaagtaatt ctgctaagcg
      841 cttagcaatc acttgggttt ccttatcttg taccaggcag tctttcaact ctactaaata
      901 gtgagagttt tgtgcatata agcccgcctt gacctgattt ttaaattttc gagtctgaaa
      961 ttgtaactta gctctgtaat attttggttc ctgcattcca atagttggac gaatttcata
     1021 attttcatat cctgcaggag caaatttttt cagcgcttga tgaagtaagt ccgtcttgaa
     1081 ctccagctgc ttatcataat gcaggtgcat gatttggcag cctccgcatt cattataaat
     1141 agtacaagat ggcacaattc gaaatttaga cttcttgttg accttcagta attttgcttc
     1201 aacaaagttg cgtctaatag aagtaatctg acaatagata tcttcgcctt tgagagctcc
     1261 tggtacaaag actaatgttt tttggtaaaa gccgattccc tcaccgttaa ttcccatgcg
     1321 cttgattttt aatggtattt tttgtttcac tttcagattc atacccctat cttatcacat
     1381 tttgagttat aatagagcta tgaaaatcac aaaacttgaa aagaaaaaaa gactctatct
     1441 gatggagctt gataatggcg acaaatgcta tatcaccgaa gatacaattg ttcgttttat
     1501 gttatcgaga gataaggtga taagcgaaga ggaattgaaa gagattcaag actttgctca
     1561 attttcttat ggtaagaatc tggccctcta ccacttatcc tttaaagcac gcactgaaaa
     1621 agaagtcaga gaatatctga aaaaatacga tattgataaa aacattgttt ctcaagttat
     1681 tgctaatctt aaagaagata agtggattaa tgatggtcag tacgcttatg ctatcatcaa
     1741 taccaatcaa ctttcaggag acaagggtcc ttatgtgctg actcaaaaac tagctcaaaa
     1801 agggatttca aaatctacta tagaagagaa cttgaaagaa tttgattttt ctgaagttgc
     1861 tcaacgtgta gctaataaac tattgaaaaa atatgaggga aaacttccat ctcgtgccct
     1921 gcaagataag attatccaga acttgactaa caagggcttc tcttattctg atgctaaaat
     1981 tgcctttgac gacttagata gccaagttga ccaagaaacg actcaagaac tcatcttcaa
     2041 ggaacttgat aagcaatata ctaagtatgc tcgaaagtat gaaggatacg aactgaagca
     2101 gcgtttaagt caagttttag cacgaaaggg ctacgatttt tcggatatag caagcgctct
     2161 tagagaatat ctttaatatt tccaagtgaa attcacagat tttaggtaat tttatggtac
     2221 aatagtaaat gataaactta taaattgtag aaagttggtt agttatgaag cttccaaaag
     2281 aaggcgactt tattacaatt caaagttata agcatgatgg gagtcttcac cgaacttggc
     2341 gggacaccat ggtactaaaa acaacagaaa acgccattat tggtgttaac gatcatacac
     2401 ttgttaccga aagtgatggt cgtcgttggg tcactcgaga accggctatt gtttactttc
     2461 acaagaaata ttggtttaat atcattgcca tgattcgtga taatggaact tcttactatt
     2521 gcaatatggc tagcccctac tatctggatg aagaagcact gaagtatatt gattacgatt
     2581 tggatgttaa aatttttaca gatggtgaaa aacgtctctt ggacgttgaa gagtacgagc
     2641 gtcacaaacg caaaatgaat tattctgatg acttggacta tattttaaaa gaacatgtca
     2701 aaattcttgt tgattggatt aacaatggac gtggtccttt ctcagaagcc tatgtaaaca
     2761 tttggtacaa gcgctatgta gaactaaaga atcggtaaag ttgtcaaagg tcaggaacaa
     2821 aatcctgacc tttttttggt agtataaaat aaattgtgta aacacaaaaa ggaataaatc
     2881 cattatagca gagttacaac acattactag aaagagattt atgcagaatt cgtaaaatat
     2941 ataaaacaaa tagatatact gttatttttt aatgtcttta ttccttgtaa gtttacaaca
     3001 aacttatatc gtttaataca aaaaatacga aaatgattag tataaaaaat acaggtcaac
     3061 ttcgtatcaa aaacattttt tgtatttcca catagcatca atcatttagt acaatatttt
     3121 aaaaacaata tctcaacaga gaaaatgcta tttaatctat cagctcattt aatcgttttt
     3181 gaaaatataa agcaatactt ttagattcaa taaactcaaa atattttatg acattgtcca
     3241 tttgtttaat tccagattct ttagattctg tcaggtatat tttatatcct tctacaaatt
     3301 tgaatgtaga tttttcaaaa acttttgtgt catcttttaa taatttttgt aatttcattt
     3361 caaagtagtc tgcatatatt agatgtgaat tttcaaggca acagaaccaa aaatttaaac
     3421 aagctgacac aactaaattt ctattcttgc cgattttttc gtaataatgt gtacgctcta
     3481 gtatttcttt tccgaccatg taaatataat ctatttcaaa aagatgatat aaattaccaa
     3541 tcagcgttat ctcataggac tcccatcgtt ccttctggaa taaatagtca tatactaaat
     3601 ttagatcatc tctactaact acatgattac aatctagctg atttaataat ccctgaacca
     3661 taattgtatt tattttatga gtttttcccc actcttctca aaaagttttt gttgctcatt
     3721 ttttattacc tccaatcctt taatattcaa actataaaaa tttggagata aattaaccaa
     3781 aaaatcatca cgctccgatc ttttataatg ttctaggtga ttacaaaaat tctctatcga
     3841 aacattgata tttgataata attcaaaaaa tgttgaaaga gaaatttcat tttctcccct
     3901 ttcaaatcta gaaagttgag attttgatat agaatcagct gcagcatact ctaaagaata
     3961 tccgtttaat tttctaaaat atcgaaaagc ttctccgtaa tttttaacac tttcattccc
     4021 ttaactttct tttcgagcga taaacatacc atattcttta ggaatgatca acataccatt
     4081 attaaactca ctttccaatc ttttacgtat tatttcctct tctagtgaac ctatttctga
     4141 aattccttta aacgattgga tgtatttaac taggtcatct atactagtta cctgtaactc
     4201 atcatcatag agtaatgtat cgacagagtt aaaacaccta ctcaggtacc ttttaccatt
     4261 ttgtaaagta aacgttttat tttctaaatc ttaattaacc tcatctttaa ataaatttgc
     4321 caaataattt acaactccat tttcaccaaa tgtagcacag taaaaaatgc cttcggtttt
     4381 taagactcta ttcacctcag agagagcttt aggaatatca ttcacatgat gtagaagcat
     4441 attagcaata acaatatcaa aagtttcatt ctcaaaagaa atcttttgaa tatccattat
     4501 ctcatagttg acattgtctc tatttccaat cactgacttc gttgatttta ccatatcttt
     4561 agaaaaatct gtaacaatca actgcttcat tttatctata gaatcactat tacttttcca
     4621 cagttctcct gtaccacatc ccaattctag aacctttact tcatcagtga tttggtagtt
     4681 agaaaatatc caattattaa atcccaattt attttttgaa tacttcttat gcaattctac
     4741 tcgaatatca agattatcat gacttttata ttgttcaatt actttatcaa tcattttaaa
     4801 tgacctccac tctataattt ggcattatat cacaagaata aagatagtgt taaataagtt
     4861 gtataaatgc aaaaaaggaa tattccgtta tagcagagtt gtaacacatt tctataaaga
     4921 aatttattcc tacgactcag tttggcatgc ttaacttcct aactcaaaaa caaagcatcg
     4981 attaatttat ccgttcttct tttgaaacag atttgaactg ataattgctc gttttaattt
     5041 aagaagctct tttgagcaaa acttcatttt tttaagagtc caacaatttc tgtctatcgc
     5101 ttttctacta gcaaaaaacc taccttgagg caagttttca tttatatatc taatttcgta
     5161 atagtatagt aaaatgaaat aaggacagtc aaatcgattt ctaacaatgt tttagaagta
     5221 aaggtgtact attctagttt caatctacta taaatttgat atgagaatag tctttctcaa
     5281 catccttatc caacaagaat gctgtggcgt ccttcttaac ttgaaccagg tcaatattct
     5341 gggcttgggc tgcatagacg cagccacaat aacattgacg atagatatca tactcctcac
     5401 acatctctac tgaacgtttg tagccttgat ttttcttgaa atcgttggga agatagtggg
     5461 tcgtgtaaat tttttgcaca tcgattccga tgctattgat ggtttgagaa ttcttatgag
     5521 gactgatggt caaggctgaa ccaaagtagt caaagcccaa gtccatagcc acttgcgctg
     5581 ttttatccag tcggtagtca aaacaaacct tgcaacggtc gccaccttcg ggctcctcct
     5641 ctagtcccct aactagtttt cggtattcat tgggttcgta gggagcttct aggtactgaa
     5701 ccgtatttcc tgtccgctca ttaaaatcac taacaaattt cttgatgaca tacacccgct
     5761 tatggtattc taccttggga tggatattag aattggcaaa atagatggtc acatctgcat
     5821 acttggtcaa atattctagt gtataggtac tacaaggggc acagcaaaca tgcacgagaa
     5881 tggttggccg ctgctcattt ttctcccatg cttgtaccat tttctgcatg acacgatcat
     5941 aattaatctt ctgattgggg ggcatcttgc acagaatttc ttctacatcg atcatgttct
     6001 tctccttttt ctagtcttat tttatcatat aagttaggag agctcaaatc aatttagaag
     6061 tgaatattat aaaaagaagg gttagtcatt ccctcctttt gtgtttttta taagttgttt
     6121 tctatagaaa gcttacatca tcccgcccat catacttgga tccattgctg gagctggggc
     6181 tactggttct ggtttattgg ctacgactgc ttctgttgtc aaaatcaagc ttgctacaga
     6241 tgctgcattt tgtagggctg aacgactcac tttaactgga tcaatgatac cttgatcaat
     6301 catgttaacc cactcgccag ttgctgcgtt gaatcctata ccaagctcag catttttcaa
     6361 acgatcgata acgatagatc cttcaaatcc tgcattgtga gcaatttgac gaacgggttc
     6421 ttccaaagca cggagaacaa tattacgtcc tgttgcttca tctcctgtca attccaaggt
     6481 agcaacagct ggaatcacat tggcaagagc tgttccacca cctgcaacaa taccttcttc
     6541 aacagctgca cgagtagcgt tgagggcatc ttcaatgcgg agtttcattt ctttcaactc
     6601 agtttcagtt gcggctccga ccttaataac cgctacacca cctgacaatt tggccaagcg
     6661 ttcttgcaat ttttcacggt caaattcaga agttgtagtt tcgatttgag acttgataac
     6721 cgcaacacgg tgagaaatcg cttcaggatt tcctgcacct tctacaataa ccgtgctatc
     6781 tttgtccacg gtcactctcg ctgcttgacc aagagcttca attgtcgcat ctttcaactc
     6841 aagaccaagg tcttctgtga taactgttcc gcctgttaag atggcgatat cttcaagcat
     6901 ggctttgcga cggtcaccaa aaccaggtgc cttgactgct actacgttga aggttccacg
     6961 aatcttgttc aaaacaagag ttggaagagc ctcgccatcc acatcatccg caataatcaa
     7021 gagtggacga ttgctttgga gaatgctttc caaaagtggc aagatttctt ggatattgga
     7081 aattttcttg tctgtaatca aaatgtacgg attttcaagg tcagccacca ttttttcgct
     7141 atcagtcacc atgtactgtg aaaggtaacc acggtcaaac tgcattcctt ctacgacttc
     7201 aagctctgtt tccataccac gtgactcttc gatggtgatg acaccgtctt tgccaacttt
     7261 ttccattgct tcagagatgt actcaccaac tttttcagaa cgagaagata cggctgcaac
     7321 ttgagcgata gcttctttat tggcaacagg gatggcgttg tttttcaaag cttcaactgc
     7381 tgcggcaact gccgtttcaa tcccacgacg aataccgatt ggatttgcac ctgctgtgac
     7441 gtttttgatt ccttcacgga cgattgcttg ggtcaaaaca gttgcagttg tagttccatc
     7501 acctgcgata tcattggttt ttgaagctac ttctgatacc aatttggcac ccatattttc
     7561 aaaatggtct tccaattcga tttctttggc aatggtcaca ccgtcattgg taatcaaagg
     7621 tgaaccgaat gacttttcaa gaacgacatt gcgacctttt ggtcccaagg ttactttaac
     7681 agtgtctgca aggatatcga caccacgaac catggctgaa cgggcatctg atgaaaattt
     7741 aatttctttt gacatactta ctttctcctt ctattcctca atgattgcca aaatgttagc
     7801 ttcgcctacg atgatgtact tttcatcgcc atctttgaca tcaagacctg cgtgggcttc
     7861 aactaagaca cgatctccag ttttaacact tggagcaacc aagtcaccgt tcaaggtacg
     7921 aacaccttgt ccagtagcca caacttgagc tgttttggtt ttttcttggg ctgagcctgc
     7981 aaggacaaag cccccaacgg tttgttcttt ttcttctatt tttaagacca cacggtcccc
     8041 taatggtttc aacatctgat ttcctccata atgagataga tattattagc actctttgaa
     8101 cttgagtgct aattcataat tctattgtat cacttggtca gaaatagtca agaaaaaagt
     8161 ctgactttct caagataaaa agcctgagac caactcagac tttttaatgt ttatactcaa
     8221 tgaaaatcaa agagcaaact aggaagctag ccgcaggctg ctcaaagcat tgctttgatg
     8281 ttgtggataa agctaacgtg gtttgaagag attttcgaag agtattaaaa tggcaattct
     8341 tcctcttcca agaccaaatc tgccaaatct tggcctgcat tattttcacg catggcacgt
     8401 tgagcacgac tttctaagag ttggaatcct gtcacaagga cttcggttac atagttcatc
     8461 tgaccatttt tctcaaagcg acgggtacgc aattctccat caacggaaat gagactacct
     8521 ttggttgcgt agcttgccaa agtttctgct agcctgcccc ataggaccat attgacaaaa
     8581 tcagcttcac gttcaccgtt ttggtctttg taacgacggt tgacagcgat agttgctcgc
     8641 gctaccgact tgtcattgtt ggttttgtgc aattctggtg tagacgttaa acgcccaatc
     8701 aagataagtt agagttagta acagtatttt aacttgcttc tctctccata tcatacatcc
     8761 tcacagaatg taagaccagc tcacgatatt tccaagcgtt gaccaggtat tcaatcactt
     8821 caggatcttc aatttcaaaa ccaaagagca gcaacaatct aaccgtgtat tcgtttttca
     8881 aaattgggac gttgtaagtc acatctaccc aatgctcaaa acctaaatct gtctgctcta
     8941 catttgctag ttcaatattt aaaatcttca tttttattcc tcctacttat ctattcgtaa
     9001 aagaagataa aaagttatga aaaaatcatt atttttttat ttttgacaat aatcaaaaaa
     9061 taaaaaggag ctattattaa caaaatgacg tttttggcaa taataccctg ccattcctcc
     9121 tccctattct tcaagaaaac gcttttttga agaatcagag caaacaaaaa aaccgcaagc
     9181 ctgagcctgc ggtgaaagaa caatttagaa agtttccttt ctatttattt aactgtaatc
     9241 aagccatctg gctctactgt gaactctggc ttgtctgcca gtgttccgtc tggtttgagg
     9301 tagtaccagc ctgttccgtc tgcggactgg acgaaggcgt ttgataccat agcgccttct
     9361 ttagcgtcta agtagtacca tgtgtccttg tacttgaccc agcctgtctt catggcacct
     9421 tcttcgttga aatagtacca cttctcagca attttcttcc agcctgtagc catttcgcct
     9481 gagttgtcaa accagtacca gttgccgtct gtgtacttct tccagcggtc tgcaagcata
     9541 tagcctgagc catcgaaata ataccaggta ccgttgattt tctcaaactt atcttttgga
     9601 taagagccgt ctgagtgtac gtaccagtag ccagtgccat ttttctgcca gcctgttgca
     9661 gcgctcaagc cgttttcgat gtcttgctta aactgctcac ggctaatgcc ccaacttgca
     9721 agatatggat atggatccac atggtctgag tggttgtttg gttggttatt ggtacagtat
     9781 tcatgcgtct tgataccttc caagtcgtct gtatcaagag tcttcggcaa acctgcttca
     9841 tctgctagat tgcgtaagag ttcgatataa aggcggtagt ccgtcatgaa ctcttcttta
     9901 gttgaatggc tttcaatcag ttcaaccgct gcgtaactct cagtattcca accgccccca
     9961 acatcccaac ttccgttgtt cacaggacct acttgcatga cacggccatt tccgacaaca
    10021 tgtgaaaaga accctagttc agggtccttt ctatagtggt aatcagcctc attttgagct
    10081 gttgagttac ggttgcctgt tgagtgggcg tgtacttgtc gataaggctg caccccaacc
    10141 tgtggcaagc ctgttcgtag tctgtttcta tcgatatcca ttactcttgt cctttccaag
    10201 cgtcgttcat ctgcttcacg gctgactcca caaaggtgtc taagtccttg tccgtcatac
    10261 tgatattgta ttttgtaagc tcagcacgga ctttagcacg agcctgtgcc agtttttcat
    10321 ctcccttgta gccagtttca gcagctacct gctccacggc atttactgca tttttggcca
    10381 agatttcaac gattttgatg gtcttttctc cacctttttg aaccaggtag tccttgactg
    10441 ccctaactac aacccctgcc aaaacgacta ggatgctgat tgctccatta gtaatgattt
    10501 cagtaatttg ttgcatttgt tattctcctt ttttcgtatc atcatctttt tcaagtaatc
    10561 gctgaaatac ttttacaatc ggctgaaaaa gagtaacatt tccttttaat ttgcggtaat
    10621 tttcaatgag agattgaaaa gtaaatgcga tgtacccgag atagattgag tacaagaatg
    10681 caaaacctgt cttttcaggc aacaaaacgg acgccggaat gaggatcatc agtaagagaa
    10741 cccctgaaat cttacgaagg agcccgttaa tgccgatttt gctcttgtac tcgatgtcag
    10801 gattgataat cgccgcaatc gtccctgtca caaaatcaat gatttccatt gagacaatca
    10861 gtgctagagc gtacaagacc aaaccatctt cagtctgtac gacacttctt agaaaattga
    10921 aaaattcaat ttgcatacac acctcctatt ctttaggttt taccgttgga tccgtccagt
    10981 caggattgcc ctctgcatca aatttcatga tatagaattc atgattcaac agaacggcta
    11041 cgttgattgt tgcgattgta ccaccccact gattgaacgc ccaaacggtt tcaacatcct
    11101 tgaattggcg acggccattt acaatcacag gacgtttttg aacgtcacga tacatataga
    11161 agtcatcgct tacattcttg caacgaatga actctccatt ttctttcatg tagcgcaaag
    11221 cactcgcaag atcaaatggt tctgtgattt ttgtaaggtc tagtaagtta tctgtgtttt
    11281 gaattgtttc tgccatgtct attctccttt gtctgctggt ttagtttgtt catcaagcag
    11341 agcttccagc tcatccactc gtgcttgaag tctttgattt tcttcccttt gctcatccaa
    11401 ctgaatactc aagatattac ttgtaatcat cgaatttgtt gaagttgttg atatttcact
    11461 aattgtcatt cgtaaggctt ggttaagctg ttctgtgttc attttctaag ttctccaatc
    11521 tgtgtgttcg ttttctattt tcaagagcaa gctcctaaat tgctttaagt gcgatattgg
    11581 tcaacatgtt gttattcatg ctattgtttt ctccattttt tctatttttt gatttaattc
    11641 ttgaatagcc ttgattaagt aaggaactaa agcggtatag tcgatatgta gatagccatc
    11701 tggattctca ggatctcgtg agacaattct tggaacgatg gtttcagcct cttgagctat
    11761 tagaccaatc tcctcatgtt tcttattttc gatgaaatca aatgcaacca ttcttaatct
    11821 gttgatttta tccaaggctt tcacagctgt atctgtgatg ttctctttta agcgtctatc
    11881 tgatttttgt tccatccaat acttcaagct accgctaccg acctgattcc accaaacaac
    11941 cgcattcctt ccgcctttgg gattccaacc atcaccaagc acatctttac ttccaacttc
    12001 gataccattt gaaaacacag gagaacgaga aaaagtagta ttcccataga agtttgctct
    12061 cgatgaattc gaaaaatcca cttgatcata aaaaccgact tcattcctac agtacatttt
    12121 cccatcagta ttgacgttcc atgctttagg tccggcatag ttccaattat ttccccagtt
    12181 cgcccagaag gctgtccgga ctccataccc ggcaccattc cccataccaa cagagaactg
    12241 attgacacct gaaatccagc gaccgccacc ctggtcaaat tgaccaagtg tgaatccacc
    12301 aattcggcct tgataggctt ctaggaaggt tgagctagaa atgacggact caaccttagt
    12361 agagaagata cgtttagata tcagttggtc aataaaagca tcagttgcag ttaatttttt
    12421 aataagcgca ttgtcaactt tcaacttctc agcagttacc gcttcagctt ctaatatcgt
    12481 agtcgtgacc gaaccagctt caaaattggc cgttttgagc ttatcaacca tggcagactt
    12541 gatgactgct ctgtcaatca gggtctctcc agtgatgtgg gtcaatttcc caactaagcg
    12601 gttatgtcca ttggcgccaa gattgattcc agagatgata tctccagccg agttgatgtt
    12661 ttcaactacc catgagccag ctagttgagt catttttgtt tgcgttgctt caagcttctt
    12721 attcgcatct gcgactgcat cttctggatg tggttgccat gttctaggtt tataaccttt
    12781 gtacaagtca acttctgtaa tatacaaatc agctgttcct gatgatgagc cattgttatc
    12841 aaaacgaatg taagcattat ccatttctcc ggaattaaaa gttactgaga catcttcgca
    12901 tctagaggta gatagtttct tgctgctaac aactttctta acgattgtga atccatcgct
    12961 ctcgcctgct cttcgtccca aaatataaac atcatagctt gcgagagcac tgttgttaaa
    13021 tcctctaaaa ttcagtacat agtcagtatt tcgttcaaga ttaaaacggt gactatacaa
    13081 aaagttttcg tttttagttg cattacttaa acgcataagg tctttctgcc cgttgtgata
    13141 aaagctatgc ttaaccaatc ttcctaaatt ttgagttgag ccccattcat tcgtagcatt
    13201 tttaaaatca ctattcttaa tgaggttagg gccgcttaca ctatatttcc caacctcaac
    13261 ctgaaacagt tgattagtca gagccatgcg agcaacctta tccgcaattc cattttcagt
    13321 attgcccaaa atccgctcgt aaagtttact ggtttcctta acacgctgga agtcagtagt
    13381 ctctactttt cgcgctagtt gattggtcac attcgcaaat tgactatcag cattcgcttt
    13441 gtttgcagaa acctgatcag atattctacc catttgtcgt tcagcattat ccttgtttgt
    13501 agcgacctga gtctttaaat ttgaaatctg attatctgtg ccttgtttat tactgtttat
    13561 ccgatttgaa agatttgaaa tctgagtagt ggttccttgc tcactgcttg taagtctatt
    13621 tgatagacca ctgatttgac cgcccacatc ttgcttataa gtagttatct gacttgaaat
    13681 atccgtgaac ttaccatcta cagattgacg atagctagcg atttgactag cgatgtcttt
    13741 attcgcacta gttttaacag cttcaatcct ctgattgata cccttaacat cttcttgata
    13801 agtagcctta ccaacgaaat cacgattgac cagctcacgg actgctgtcg cttgtctcgc
    13861 gctctcctca cgagtatagc gctgtagggc ttcctgtcgc tgaccgtctt tatttacata
    13921 ttcctgaata gctgataagt cggttcgcaa gccctgagct gtccgctcaa aggtagcctt
    13981 agcctcagtg atgagaccat cagcgtcctc aggcgcagga ctccagtccg tcgccacact
    14041 accgatttca accttgattc ctgttaccca agctgtaccg cttgtagcac cttcaagatt
    14101 gaatcgcaat gatgtcttca attgatcaaa atttgttttt tcagagtagt cataagtgaa
    14161 tgtaatatat ttccaatctg ccgaaccttt atacatacca agcgtagcat aatctggacc
    14221 actctgtact ccggtctcac tattttttct aaaaagataa tgtttgaagc aattaaatac
    14281 attccaaaaa tttcgacctt ggactacatt ttcgtacttg atccaagcgc taaaagtaac
    14341 tttttggtac aaccttgagc tgaaatctgg ttcaaggttg aacgttaaag tagagttgtt
    14401 ctctagccta tagcattctt tttgacctgt gacgtggttt tcaggtaatt tttcaattac
    14461 agctccaacc gtcttggatt ttatccatag attccggcct cccaccttca tttttgaaaa
    14521 ttcttcacgc aatttcccgg cttcagatac aactaaagtc ttatctgctt tatccttggt
    14581 tgcgttcagg atttcctgac ggatagagcc agctcgcacc tcaaattcag cctgactcaa
    14641 cttctgattt agcttgttct gcgtgtctgt ctcaagactc ttcaccgact gcctaatatt
//
LOCUS       DBERMR010000049        14454 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.049, whole genome shotgun sequence.
ACCESSION   DBERMR010000049 DBERMR010000000
VERSION     DBERMR010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 14454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14454
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.049"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            1..165
                     /locus_tag="ACL8A1_001697"
                     /pseudo
     CDS             1..165
                     /locus_tag="ACL8A1_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(320..604)
                     /gene="tnpB"
                     /locus_tag="ACL8A1_001698"
     CDS             complement(320..604)
                     /gene="tnpB"
                     /locus_tag="ACL8A1_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGS1202431.1"
                     /translation="MCGKTDMRQGIDSLAYLVKTHFELDPFSGQVFLFCGGRKDRFKV
                     LYWDGQGFWLLYKRFENGKLTWLSTEKDIKALTSEQVDWLMKGFSITPKI"
     gene            complement(624..810)
                     /locus_tag="ACL8A1_001699"
                     /pseudo
     CDS             complement(624..810)
                     /locus_tag="ACL8A1_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            896..1003
                     /locus_tag="ACL8A1_001700"
                     /pseudo
     CDS             896..1003
                     /locus_tag="ACL8A1_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutT"
     gene            complement(1000..1515)
                     /locus_tag="ACL8A1_001701"
     CDS             complement(1000..1515)
                     /locus_tag="ACL8A1_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HGS1202432.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGVHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(1527..2054)
                     /locus_tag="ACL8A1_001702"
     CDS             complement(1527..2054)
                     /locus_tag="ACL8A1_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGS1202433.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            complement(2336..2899)
                     /locus_tag="ACL8A1_001703"
     CDS             complement(2336..2899)
                     /locus_tag="ACL8A1_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGS1202434.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILSLAIVPRLQTLKK"
     gene            complement(2883..3434)
                     /gene="coaC"
                     /locus_tag="ACL8A1_001704"
     CDS             complement(2883..3434)
                     /gene="coaC"
                     /locus_tag="ACL8A1_001704"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HGS1202435.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLSLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            complement(3446..4135)
                     /gene="coaB"
                     /locus_tag="ACL8A1_001705"
     CDS             complement(3446..4135)
                     /gene="coaB"
                     /locus_tag="ACL8A1_001705"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HGS1202436.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISADQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            4365..6035
                     /locus_tag="ACL8A1_001706"
     CDS             4365..6035
                     /locus_tag="ACL8A1_001706"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HGS1202437.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNALSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            complement(6526..7692)
                     /locus_tag="ACL8A1_001707"
     CDS             complement(6526..7692)
                     /locus_tag="ACL8A1_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HGS1202438.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFHVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASEFEPQYCLATSRRANAHYVY
                     SEENLLAKYSQQFKVKKIMPEAFAELQGDYFYMLTTPSRQEMDQMIADFGQRQE"
     gene            complement(7705..8444)
                     /locus_tag="ACL8A1_001708"
                     /pseudo
     CDS             complement(7705..8444)
                     /locus_tag="ACL8A1_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            complement(8466..9298)
                     /locus_tag="ACL8A1_001709"
                     /pseudo
     CDS             complement(8466..9298)
                     /locus_tag="ACL8A1_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(9434..10648)
                     /gene="thiI"
                     /locus_tag="ACL8A1_001710"
     CDS             complement(9434..10648)
                     /gene="thiI"
                     /locus_tag="ACL8A1_001710"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HGS1202439.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYPQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSAVQEIMQDIYKE
                     GMTFKISSKRSDHTFELDSRELNRTLGGAVFEAIPNVQAQMKSPDINLQVEIREEAAY
                     LSYETVRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            complement(10657..11799)
                     /locus_tag="ACL8A1_001711"
     CDS             complement(10657..11799)
                     /locus_tag="ACL8A1_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HGS1202440.1"
                     /translation="MIYFDNSATTRPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWIIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKAQGFEVDFAPVDNKGFVDVEALEDLIRPDTTLVSIMAVNNEIGSVQPIEAI
                     SKLLADKPTISFHVDAVQALAKIPTEKYLTERVDFATFSGHKFHGIRGVGFIYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     DYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAQSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            complement(11959..12336)
                     /locus_tag="ACL8A1_001712"
     CDS             complement(11959..12336)
                     /locus_tag="ACL8A1_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202441.1"
                     /translation="MKHKIMKKIIVVVFAIPWLTFLSQVHADYVSKSSSLDGYSLVGL
                     NVLNVTFNASGYSDTYSGSVSNMYFTDWAWFPNTISNRSTWKSSIPYGQRANGTVHIG
                     VGVNSPWGAVNLWGGQNYFSLDF"
     gene            complement(12346..>14454)
                     /locus_tag="ACL8A1_001713"
     CDS             complement(12346..>14454)
                     /locus_tag="ACL8A1_001713"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS1202442.1"
                     /translation="DFKKGGRYMFEIRNLSLSFAGRRLLEPTDLIFERGKVYTIYGKS
                     GVGKSSLLNKIGLLSATDPSVSYFFDGKEIDIENKKSVSVFIAEEVAFVFQGRNLIND
                     LTVFENLRLALNFYDLSPDEIESKIDTVLADLQISSLKHAYPEDLSGGEEQRVAIARA
                     LVTGKTLILADEPTSSLDKENREKVSALLIDLAHKYHKIVLIVSHDEAMIAIGDVQLH
                     FKDHKLVGNCLILNSDEGIKDSKKWSSCLSSSHTKLSVLKHRRPFLPRLLAFFISLVV
                     AIAVTSINFQSLFADKYHQLVNNSLENGFLVINDSLHLQTTKVLDDFLSFDKEEITSI
                     STSQNIQTVNPYIEFLSLGMTFDNSKDYSQLQKNFQPTLTIDGQTRSLTRNFSIQPLY
                     QTNTTQRQIEYFDRSNEKGIYLSEQFISDEKLPNIVSGTTVTLTFYIPISLYESSIEK
                     EGNILKGDGDLFVKVDKTYPVLGIVKKSYPFEYSPYGNTLFMNIAEMEELQNEAIQQH
                     PMTKMALDGFPLKSWMPSAIHVLLKDSGAIPEELSRIRAISSQITILSSYENYEEFNK
                     GLTYIRQFLMLLSLVLLILVIAVLSFVFFLLNRPRRFEVGILKSLGYSTQNIVWLFLK
                     ELIGYGKTISILASCLLVILSILAMQVLKLEISDVFKFYLTSVFTLIGLSVSVLIISG
                     LLPIYTTCRQTVVDTIRKNG"
BASE COUNT         4377 a         2877 c         2858 g         4342 t
ORIGIN      
        1 caagtgtgga aagagattcg taaacgtgga tttaagaata aagcctttta aactttggaa
       61 gatgtcatga atcaactcca agatgttata caaggactgg agaaggaggt gataaagtcc
      121 atcgttaatc ggagatggac tagaatgctt tttgaaaaca gatgagtata aaaagaaagt
      181 cctcatttca atagaaatca caactttctg atggatttat agtaaaatga aataagaaca
      241 ggacaaatcg atcaggacag taaaatcgat ttctaacaat gttttagaag cagaggtgta
      301 ctattctagt ttcaatctac tatatttttg gagtgataga aaagcccttc ataagccagt
      361 ctacttgttc agatgtgaga gctttgatat ccttttctgt acttagccaa gtcagttttc
      421 cgttctcaaa gcgtttatat agtagccaaa atccttgacc atcccagtaa aggactttaa
      481 agcggtcttt acgtccacca caaaagagaa agacttgacc ggagaaagga tccaattcaa
      541 agtgggtttt aaccagataa gccagtgaat cgattccttg tctcatatca gttttcccac
      601 acacgagata gacctgccct aggctagata gatgaattgt tatagagcaa caccttatcc
      661 aaaagctgtt ctaccatttc gagattgaga gattgaaaaa aaacttactt caagcttgcc
      721 aatacgaatt ttaagcagaa tatcatttct ctttttagaa tcaaaaggac gagattgtgg
      781 aatctctaca ggaacaatag gttgtggcat taaaatatcc tccaatagtt ttacttcact
      841 aatagtatac aggaggagct ccatgaatga taaatacctt gttatgacgc gcttactctt
      901 ctatgatatg ctaccattga tggaaatgat ggaagctccc gacaagtcag agtttttcta
      961 ccctcgccgt acagaagacg attgggaaaa gaaaatcttc tagtctttta ctaaataacc
     1021 tagctgatcc aaggcctcct cgatatagtg gaggtcttgt tgtgtttcgg cttcgactag
     1081 gtggtaatgg acgccatctg tcaattcaga tagaggtcta aagtcagaat gctcgacttg
     1141 ttctagaaaa tgttgcacat ctctgcgaca agacagttta agcaaggttt caatttctcc
     1201 ataaacggga tggtcaatca aggtattctg gacatgcccg ccattatcga caatagcaag
     1261 gagttcctga ccgatttctt caacttcatg tttcactttg aaaagtttgt gaacataagg
     1321 attggtatca atttgcttat agatgtagcc acgattggtg gataggatag gagcgccatc
     1381 ggctctcaaa attgcaatgt cctgcacaat gacctgacgt gtgacatgaa aatgttccgc
     1441 caaactttgg ccattaaggg ctttaggagc ttctttcaag agttggagca gggcttgttt
     1501 gcgatctttt gtcatagctt ttccttttaa cggcgttttc gaagcacttt atagacagct
     1561 agtgctaatg tatagtctac catactatgg ataattgtgc caaatccaac tagtacaaat
     1621 agaacataga acatgttttc tacattggta cccgaagttg cgtaaaaaag gacacaggct
     1681 aatacttcag caagggcatg aacaacagcc aaaacaaagt tgaaaaccca ggaagatttt
     1741 ggtttatcta gggtatcggg gaatttttgt aggtaaagag ctcctaaagc accaaaagat
     1801 atatgggaaa aagcccgaaa aacgataacc atgggatagc cagccatcaa aaatccaaaa
     1861 ctagaggcta ggatgacaaa aactgccatc aagggcgaca agaacatggc tataaaaata
     1921 gcgatgtggc tccccaaagt ataggaagca ggtggaatga caatcttgaa aggcataaca
     1981 attggaatca aaatcgcaat agccgttaaa agggctgtca ttgtcataaa ttgtgtcttt
     2041 ttccgtgtat tcacaagaat ctccttttta actgcatata cactagtatg gtacaataaa
     2101 ccagacaata aagcaagaat ttacttgggt ttatagatca ttttttagtt aaaagttata
     2161 gtagattgaa actagaatag tacacctcta cttctaaaac attgttagaa atcgatttga
     2221 ctgtcctgat cgatttgtcc tgttcttatt tcgttttact atagtaaaga tttcattaaa
     2281 aagaaactgt atagagcaaa atctccacct tcaggtttga aagcggagat tgtttttatt
     2341 ttttcaaggt ttgtagtcgt ggaacaatgg ctagggatag aattgcagaa atgaccaatt
     2401 cagcaattga atttgttgag ataacggttg ccagaagaag ttggatattt ccattataaa
     2461 catttccaaa taggaagaag attcctccaa ggacaaagat agtatttgtc aaggaaccaa
     2521 gggccccagc taaaatcaga ccagtcttgt ttttcatcag tttatagact aagtaaggag
     2581 ttaaaccaat caaaatacgt gggacaatgg caatgatagc tgagtagatg tttccgtttg
     2641 gtacgaaggg agagaagagg tagcttgtcg gtagaatcgt aatcgtgtta accgtcaagc
     2701 taagtaatcc catcaaaaat ccaagtgtaa ccccaactcg tggaccataa ataatgctgg
     2761 caataatgac aggaatatga acaatggtcg gtttgattgg aaatggaaaa aggttaaaga
     2821 taagtgagct cagaaagtgt atcacgagca tggtagcaaa aaagatagca atgggtgcaa
     2881 tattagagcg ttttttcatc gatagtttcc tttattcttt ctaaaataat tgtgaggtca
     2941 gctaaagctc ctcgtccgtg gtctccacaa gctagtaggg attccttagg agcaatcagc
     3001 tgatagccgt aggtttctaa tgttttcaga ttattctgag ttactggatg gtcatacatt
     3061 tttgtattca tagcaggagc tattagtttg ggaatatgac ttggtagggc tagagctgta
     3121 ctggttacca tgttgtccgc aaagccgtga gctagttttg caatagtgtt agcagttgca
     3181 ggtaccacga taaataaatc tgcttttttt ccaagttcga tatgattgac ctgatcagga
     3241 tagggttcct tcatgacatc caagtggaca ggattctgtg agagtacctg tagtgacaaa
     3301 ggttggataa actctgtagc agcctgagtc attaagacag tgacttgatg gccttgtttt
     3361 tttagagaac tgactaaatc tgccgactta taagaggcga ttgaacccgt tacagccaag
     3421 agaatgtttg ccatagtttt cctttctaag aatgataggc ttgaattttt tcaaggagga
     3481 gttctgcaat ttcttcttta gtctggactg tttgaagctg atttttctca acaaatatag
     3541 ctcggtgctg atctgctgaa atttgagtca ggtcattcgc gatgattaaa tctgcttgat
     3601 tcttgataag actttttcgt gcaatatcaa ccagatgatc ttcggtaaca tcaaccagca
     3661 gtttgaaacc aatcagatga atagtaggat tccattcctt gactagggat atgattttgg
     3721 gtgtcttttt aaggaacaaa acctgaacct catcagttga agaaatcttg gcctgatgat
     3781 tctgcttgct taaaaattct tttagattgg agctagcctg aacttcctca agccctgtca
     3841 tataaacagg agtgtagtca gaaacagcca ttgagtggat caagacctga taatcctgaa
     3901 cacgttcttg catttctatt agaaggtcct tggtattggt aatttctcga atacttaggt
     3961 taggatgagg ctctggcttc agagctcgtt ttgtcgtaat taaacaaact tcatgccctg
     4021 cagaaagcaa agtctctgtg ataattttcc ccaagtgacc tgtagaatgg ttagtgatag
     4081 agcggacgct atcgatagct tcactggtac cgcccgatgt aactaaaatt ttcatagcac
     4141 tattgtacac aaaaataagc ggtaagtaaa atgaaagtga taaatttttg gtaaaaacga
     4201 agatttacta tagtttcaaa ctataaagcc atataaaatt taagaaagga ctctaaaagt
     4261 cttaaaaaca gagatattta cgaacgttta gagagtttta agatgttaaa aatcttgttt
     4321 tgtgatataa tgaattatca tataagaggt tagaggaatt ttgaatgaaa acagatattg
     4381 aaatcgcaca gagtattgag ttgaagccaa ttgttgatgt tgtagagaaa cttggtattt
     4441 cttacgacga tttggagttg tacggaaagt acaaggctaa actcagcttt gataaaattc
     4501 gggcagttga gagcaatcca gtcggtaaat tgatcttggt tactgccatc aacccaacac
     4561 ctgcaggtga aggaaagtcg acgcttacca ttggtcttgc ggatgccttg aacaagattg
     4621 gcaagaaaac catgattgct atccgcgaac cgtctcttgg tccagtcatg gggatcaagg
     4681 gtggtgctgc tggtggtggg tatgcacaag ttctgccaat ggaagacatc aacctccact
     4741 ttactggaga tatgcatgct attacaactg ccaacaatgc cctttctgcc ttgattgaca
     4801 accacttgca ccaagggaat gagctgggaa ttgatcaacg tcgtatcctc tggaaacgcg
     4861 ttgtggactt gaacgaccgt gcgctccgcc atgtgactgt tggtcttggt ggtcctctaa
     4921 acggtattcc acgtgaggac ggttttgata ttacagttgc ttcagaaatc atggcaattc
     4981 tttgcttggc aacggacatc gaggacttga aacgtcgttt ggcgaatatc gttattggtt
     5041 atcgctatga ccgtacgcct gtttctgtag gtgatttgca ggttgagggt gccttggctt
     5101 tgattttgaa ggatgctatt aagccaaact tggttcagac aatttacggt acacctgcct
     5161 ttgtacacgg tggtccattt gccaatatcg ctcatggctg taactctgtt ttggcgacga
     5221 caacagccct tcacttggct gattacactg ttactgaagc tggttttggt gcggatcttg
     5281 gtgctgagaa attccttgat atcaagacac caaacttgcc aacatctcca gatgcagttg
     5341 ttattgtcgc aacccttcgt gcccttaaga tgaatggtgg tgtggctaaa gacgctctga
     5401 ctgaagaaaa tgtagaggca gttcgtgcag gttttgctaa cttgaaacgc cacgttgaaa
     5461 atatccgtaa gttcggtatt ccagcagttg tagctattaa cgaatttgta tctgatacag
     5521 aagctgaaat tgcagtctta aaagaactct gtgcctcaat cgatgtacca gttgaattgg
     5581 ctagtgtctg ggctgatgga gcagaaggtg gagtagcact tgccgaaaca gttgttaaga
     5641 caattgctga aaatccagct aactataaac gtttgtacga taatgccctt tctgtccaag
     5701 aaaagattga aaaaattgtc actgaaatct atcgtggtag caaagtgaac tttgagaaga
     5761 aagctcaaac acaaattgct caaatcgttc aaaacggttg ggacaaattg ccaatctgta
     5821 tggctaaaac tcaatacagt ttctcagaca atccaaatgc gcttggagca ccagaaaact
     5881 ttgaaattac cattcgtgaa ttggtaccaa aattaggtgc aggcttcatc gttgccttaa
     5941 ctggtgatgt tatgaccatg ccaggtcttc caaaacgtcc agcagctctc aacatggatg
     6001 ttgaaagcga tggaactgta ctaggcttgt tctagtggat tgaaattgac tttaagtgag
     6061 tttggaaata atatccaagc tcattttttc tcagactgca agcagttcat caataagatt
     6121 tatactcttc gaaaatctct tcaaactacg tcagcttcac cttgccgtag gtatatgata
     6181 ctgactctgt cagttctatc cacaacctca aaacagtgtt ttgagcaacc tgcggctagc
     6241 ttcctagttt gctctttgat tttcattgag tattaataga cttgtttggt atcctgaaaa
     6301 ctatatcgta taggtgtcgt catggatcct tttagctgat ttaaggatac gaaaacacaa
     6361 tactatgact aaattgaaat gttaaaattc aatttctagc cacctaatcc tgcttcaaat
     6421 agttatcgaa caagttttat gatagctacc ttgttaggat gctcttattt tttaccagac
     6481 aaaacaaaag gattaaggga gtgaatttcc ttaatccgat agttcttatt cttgacgttg
     6541 tccgaaatca gcaatcatct gatccatttc ttgtcgactc ggagttgtca gcatataaaa
     6601 gtaatctcct tgaagttccg caaaagcctc tggcatgatt tttttaacct tgaactgctg
     6661 gctatatttg gcaagtaagt tctcctcaga atagacatag tgagcatttg cacgacgtga
     6721 tgtagccaaa caatactgag gttcaaactc tgacgctggg aactcttctc ctgcgacaat
     6781 ggctgcatat ccacgataga ggtccaagga atgagcaaag ttataaacat caatggtgaa
     6841 accacctgca gggcggttat tgtactcgat ggtaatataa tcgtccccct cacggaagaa
     6901 ctcaatatgg aaaaaccgtt ctttcatacc aaattctttg acaattgctt ccccatactt
     6961 gcgcagttta ggatccatat ccttgagcac ataataagaa ttgtccatct tataaatcat
     7021 gaggtcaagc ggtgtatagg cgtagtcaaa ggttgttgag aagacaatct ttccatcctt
     7081 gtccacgagc ccgtcaaagg tacagatttc gctggaagtg acaaattttt caaagaaata
     7141 aagggttgaa tggtcccatt cttgcttgaa gtgattgata tcgtcttctg tctcaagttt
     7201 aaaggttgcg gctgctccca ctccattatc aggtttggca atcattggaa gaccgatttc
     7261 tttcactgct tgatcaacat ctgcttccgt cttgataaca gctccaggta ccacagggac
     7321 acctgctttt ttgaaaagtt tcttcatttc agacttatat ttcgtctttt tgagatcctc
     7381 tggtttggca ccaaaaacat tgaattgttc tctgagtgtt gcgtctagct caagccagta
     7441 ttcattgtga gactcgatgc ggtcaattgg accatgttta taaaagagaa aagcaactgc
     7501 acgtttgact tcatctatgt tctcaagatt atcaacatga aaatactcgg tcaagctatt
     7561 gcgcaagggc tcatccaatt gctcgtaaga ctcttgacca attcccaaga ctgtgatgcc
     7621 tttattagct agttcgatgg taaactgttg aaagttttgt ggatagtagg gagaaataac
     7681 aaggtaattc ataggtaact ccttttataa atagagatta ccgaggaaat aaggcatttg
     7741 tttacgccac cattcccagt catgggcgac atcatgtccc cattcagcaa accaggctgg
     7801 aatttgtttc ttgtcaaagg cttctttgag cttgtaaaag gatggcaaac catcttgttc
     7861 ccaggctcca agccccgtac acagcacaat ctctgcctga cggtaacggt caataaacca
     7921 gccgtcgttt tggttccaaa tataatctac tggcgagttt tggtaaatag catcatcgtt
     7981 gtagtaatca ccgacaaaga aacgtgcgtc gtaaacacca ctgagagcaa tcactttggt
     8041 aaagacatct ggatgctgga ggaagaaatt gagtgcatga taggctccca tagagcaacc
     8101 tgtcgtcatc atgccatcaa accaacctgt cttgtgcttg ataaaagaat ggcctcctca
     8161 atcacataac attcgtaggc acggtgcatt tccgcttggt catgagcatt tttccaagta
     8221 gccaaccagc tctcactatc caaactagat agggtaaaga actggacacg gccttcctcg
     8281 ataaaggaag cacaggcatc aatcatgcca aaatcatagt attcgttgtg actactacct
     8341 gatgaagcaa agaccacaac tggaatccca ccatgtccat aacggttaag gtacatttca
     8401 cggttaggat gaccactcca gtggctaaga tgttcaatat gcatttcgtt ttttcctttc
     8461 ttaacttacc atttttctgc aaaaaatctc agacaatctg gtagattttc tgaccaaggg
     8521 atttcactat ggatggcacc agactgaact tttagcacaa gattatccag atgtactccc
     8581 cctgctatca aatcatggta atagcaaagc gacgagtcga tataggcttg tttgatattg
     8641 ccatccatca aggtcttgtc tgtatcatct gcttcttctg ttcctacata gatgaagatg
     8701 cgctggtcag gcgatagttt ctggcactcg atatagcggt taaaggcttc ttggtggagc
     8761 cagtttgcag atgaaaaaac gcccaagcaa ccaatttggt cttggtattc caaaccgata
     8821 aactgggtaa tattgcctcc tagtgaggaa ccaatcatag ccgtatgctg gcagtctgct
     8881 tttgtacgat aggtctcatc gataaaaggc ttgaccacct ccatgacaaa ctcagcatac
     8941 tccacaccct taccaccaaa ctgctgctct gggataggaa attcttggaa cttccaagcc
     9001 gcatactcat tcattcgccc cataccatca ttgtcaatag caacgacaat catgcgactg
     9061 atatactgat atccggattt cgtttgatag ctgggataat cttccatgaa tatccaatga
     9121 aagactcttt gctataaaaa acattttgcc cgtcatgaaa gtatacaaca ggataggaac
     9181 ggtctgtatc tttctcataa tctttaggaa gaagaatacg tacacggcgc tccttacctg
     9241 tataaggaac cttgagtttg tgttctttca tttttagata aaagtaggat tgattcattg
     9301 tcagaaaact ctctatattc aaaattttat atcattatat catgaaaata gaaaaaagct
     9361 agttttttac ctgtttttag aaaaaaaact aacttttttt actgattttc gctattcttt
     9421 tggattttct gaattagagt agattatcaa tcaagtcatc aacttcatct ttttcggctt
     9481 gaggtgtgat ttcagtaatc atgattccag ccactgctcg ctcaaccaag ccttcaacat
     9541 ccatacgcgc ttcgtactgc tccgcattct taattttagg atttgttttt ggacgatctg
     9601 gtgcaaaaat ggtacaacag tcttcaaacg gttggattga aatgtcaaag gtatcgattt
     9661 cctgggcgat gtcaatgatt tccaacttgt ccatggtaac cacaggacga atgatgggag
     9721 tgttggtaac agcattgata gccttcatac tttcaagggt ttggctggct acttgaccta
     9781 gactttcccc attgatgata accaaaccat ttcgtacctc acgaatacgg tcagtaatac
     9841 gcatcataaa acgacgcgtc aaggtcataa ggtaggcttc tggcgctttg gctttgattt
     9901 cctcttgaat ctctgtaaaa ggaacttcga taaactggat atttccccca aatttggtta
     9961 atttacgggt caaatcttgg gcttttttaa gagcaccagg actcgtgtaa ggtgggctgg
    10021 caaagtgaac tgcctctata tctacccctc gtttaagcgc tagatagcca gctacagggg
    10081 aatcgattcc ccctgacaac atgagcatgc ccttaccaga agttcccact ggtaatccac
    10141 ctgcccctcg aacggtttca taagaaagat aggctgcttc ttcacgtatc tccacctgaa
    10201 gattgatgtc aggacttttc atttgagctt gtacgtttgg aatggcttcg aatacagccc
    10261 ctccaagcgt tcggttgagt tcacgactgt ccaattcaaa ggtgtggtcg ctacgcttgc
    10321 tagagatttt aaaggtcatg ccttccttgt agatgtcctg cataatctct tggacagcag
    10381 acttcagaac ttctacagat ttttcaacct tataaacagg ggaaaagttt tgaattccaa
    10441 atacttgttt gagcgattct gctactgctg tgtaatctgc tccattgagg taagcgtggg
    10501 cacggtcgcg atctgctgtt accttaactt ggggatagat agacaaaacg tctgaaatat
    10561 tattgcgaag tttattgatg aaacgcatac gatttttgcc cttggttgac agctcaccgt
    10621 agcgaatcat aatttctgaa tactgcatga atgctcctat cttacttttc tagtttgatt
    10681 gtaaattaat tttagcttgg tcaagaactg ctcgacctga ctcatatcat tttcaaggtc
    10741 tagactaaga cgcacagctg actgagcctt atccttgtcc actcccatag caatcagggt
    10801 tccggctggt ttcccagcct tggacgaaca agccgaggtc gttgagatga aaatatcata
    10861 gtcttcaaag gcgtgaacaa tgacctcacc acgaacaccc ttgattccaa aagtcagaat
    10921 atgaggggcg aagtcttcct catctgaaaa gacaaatata tccggatagt ccagaagtgc
    10981 ttgacgaatc actgccttca tctgcccagt cttgctccta aagatatcta gcttttccat
    11041 agataaacgg agagccttag cggtcgctgc aattcctgcc acattttcag ttgtcgaacg
    11101 gtaatcacgt tcctgaccac cacctgttaa aagaggcgta atcttcttgc cagacttgat
    11161 atagataaaa ccaacaccac ggattccgtg gaacttgtga ccagagaaag tcgcgaaatc
    11221 cactcgttct gtcagatact tttcagtcgg aatcttggca agcgcctgaa ccgcatcaac
    11281 gtggaaggaa atagttggct tgtctgccaa tagttttgaa atagcctcaa tgggctgaac
    11341 agagccaatt tcattgttaa ctgccatgat ggaaacgagg gtcgtatcag gtcttatcaa
    11401 atcttctagc gcctcaacat ccacaaatcc tttgttatca actggagcaa aatccacttc
    11461 aaaaccttga gctttcaacc agagggctga ttccttaact gctggatgtt caatggctga
    11521 tacgatgatg tgcttgccaa actgggcttt ttcaaatgct actcccttga tgatccagtt
    11581 atccccttct gtcccaccag atgtaaagaa gatttcatca cttttcttac cgattaaatc
    11641 tgcaatctgt tgacgggaag catctaaaat tcgtgttgcc tggtctccca aacgatggag
    11701 actagatgga tttcctaaaa tttttgaagc cacctgcata taggtttcaa gggcttctgg
    11761 atagggcctg gtcgtcgccg aattatcaaa gtaaatcatg tttctcacgc tttctaaaat
    11821 cactccttct attgtatcat gaaacggagc ctgcgacaag aaagggcaat gtttgcatta
    11881 atctgaataa cataaaatac tcttgatacc atcatcaaga gcattcttat tcattctatc
    11941 taactttgat tatttctgtt aaaaatctaa agaaaagtaa ttttgtccgc cccagagatt
    12001 aaccgctccc caaggcgaat ttactcctac acctatatgg actgtaccat ttgcgcgttg
    12061 gccatatgga atcgaacttt tccaagtgga acgattactt atcgtattag gaaaccaagc
    12121 ccagtcagtg aaatacatat ttgacacact gccactataa gtatcagaat agccagaagc
    12181 attaaaagtc acattaagaa cattcaagcc taccaagcta taaccgtcta aagaactaga
    12241 cttacttaca taatctgcat gtacttgact taaaaatgtt aaccatggga tagcaaacac
    12301 taccactatt atttttttca ttatcttatg tttcatgata tcacctcatc cattctttcg
    12361 gatagtatcg acaactgttt gcctacaagt tgtgtatata ggtagtaagc cacttattat
    12421 cagtactgag actgataatc caattaaggt gaaaacggaa gttagataga atttaaaaac
    12481 atctgaaatt tccaatttaa gcacttgcat tgctaaaatg gaaagaatga cgagtaaaca
    12541 acttgctaat atcgaaattg ttttaccata accaattaat tcttttaaga aaagccaaac
    12601 aatattttga gtactgtatc ccaaagattt taaaatccct acttcaaagc gtcgaggacg
    12661 attgagcaaa aagaaaacaa aggacaatac tgcgatgaca aggatgagta agactaggga
    12721 tagcaacatt aaaaattgcc tgatataggt gagtccttta ttaaattctt cgtagttttc
    12781 atatgatgat aaaatagtaa tttgtgaaga gattgctctt attctactca attcttcagg
    12841 aatagcaccg ctatccttta gtaacacatg aatcgcactc ggcatccatg atttcaaagg
    12901 aaaaccatct aaagccatct tcgtcatggg atgctgttga attgcttcat tttgcaattc
    12961 ttccatttca gcaatattca taaatagggt gttcccataa ggggaatatt caaacggata
    13021 acttttttta acaatcccta aaacaggata tgttttatcc actttgacaa ataaatcgcc
    13081 atctcctttc aatatattcc cctctttctc tatacttgat tcatataaag aaattggaat
    13141 ataaaaagtc agtgtcacgg tagttcccga aactatattt ggtaattttt catctgaaat
    13201 gaattgctca gatagataaa tccctttttc attgctccta tcaaaatact ctatctgcct
    13261 ctgtgttgta tttgtctgat acaagggttg aattgaaaaa tttcttgtca agcttctggt
    13321 ctgaccatca atagtcaaag ttggctgaaa gttcttttga agttgtgaat agtcttttga
    13381 attatcaaac gtcataccca aagagagaaa ctctatataa ggattgacgg tttgaatatt
    13441 ctgacttgtg ctaattgagg tgatttcctc cttatcaaaa ctgagaaaat catccaacac
    13501 ttttgtagtt tgtaaatgaa gcgaatcatt aatcaccaag aagccatttt cgagtgagtt
    13561 gttcaccagt tggtgatact tatctgcaaa taaggactga aagttaatag aggttactgc
    13621 tatagcaact acaagagaga taaaaaacgc taataatcta ggtaaaaaag gccttctatg
    13681 tttcaaaacg gatagcttcg tatgagaaga cgacaaacag gaggaccact tcttagaatc
    13741 ctttatccct tcatcagaat tcaaaataag acaattccca actaatttat ggtctttaaa
    13801 atgaagctga acatctccta ttgcaatcat cgcctcatca tgcgaaacaa tcaatacaat
    13861 tttatgatat ttgtgagcta aatcaattaa caaggcggag actttttctc tattttcttt
    13921 atccaaagaa cttgttggtt catcagctaa gatgagagtc tttcctgtaa ctaaggctct
    13981 tgcgattgcc actcgttgtt cttcaccacc ggataaatct tctggataag catgctttaa
    14041 agaagaaatc tgtaaatccg ccaatactgt atcaattttg ctttctattt catctggact
    14101 caagtcataa aagtttaaag ctagtcttaa attttcaaat acggtcaaat catttattag
    14161 atttcgcccc tgaaatacaa aggcaacttc ttcagctata aagacagaga ctgatttctt
    14221 attttcgata tcaatctctt ttccatcgaa gaaataagaa acactaggat ccgtagcaga
    14281 gagcaaacca attttattca gtaaagaaga ctttcctacg cctgacttcc catatattgt
    14341 atatactttt cccctttcaa atatcaaatc tgttggctct agcaaccgcc tgcctgcaaa
    14401 agagagactc aaatttctaa tttcgaacat ataacgccct ccttttttga aatc
//
LOCUS       DBERMR010000050        14226 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.050, whole genome shotgun sequence.
ACCESSION   DBERMR010000050 DBERMR010000000
VERSION     DBERMR010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 14226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14226
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.050"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..240
                     /locus_tag="ACL8A1_001714"
     CDS             <1..240
                     /locus_tag="ACL8A1_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGS1202443.1"
                     /translation="LMKICPQAQTARKSSKLKPLTAEDKAYNHALSKERSKVENIFAK
                     VKTFKMFSTTYRNHRKRFGLRMNLIAGIINHELGF"
     gene            complement(555..2246)
                     /gene="argS"
                     /locus_tag="ACL8A1_001715"
     CDS             complement(555..2246)
                     /gene="argS"
                     /locus_tag="ACL8A1_001715"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HGS1202444.1"
                     /translation="MNTKELIASKLSSIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            2382..2828
                     /gene="argR"
                     /locus_tag="ACL8A1_001716"
     CDS             2382..2828
                     /gene="argR"
                     /locus_tag="ACL8A1_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HGS1202445.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            2879..5413
                     /gene="mutS"
                     /locus_tag="ACL8A1_001717"
     CDS             2879..5413
                     /gene="mutS"
                     /locus_tag="ACL8A1_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HGS1202446.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKAMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            5560..7254
                     /gene="patA"
                     /locus_tag="ACL8A1_001718"
     CDS             5560..7254
                     /gene="patA"
                     /locus_tag="ACL8A1_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HGS1202447.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLDALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQDGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(7404..7853)
                     /locus_tag="ACL8A1_001719"
                     /pseudo
     CDS             complement(7404..7853)
                     /locus_tag="ACL8A1_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844241.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8045..9811
                     /gene="patB"
                     /locus_tag="ACL8A1_001720"
     CDS             8045..9811
                     /gene="patB"
                     /locus_tag="ACL8A1_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HGS1202448.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTHVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            9950..10639
                     /locus_tag="ACL8A1_001721"
     CDS             9950..10639
                     /locus_tag="ACL8A1_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HGS1202449.1"
                     /translation="MARTVVGVTANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            10643..10822
                     /locus_tag="ACL8A1_001722"
     CDS             10643..10822
                     /locus_tag="ACL8A1_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202450.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            10809..12158
                     /locus_tag="ACL8A1_001723"
     CDS             10809..12158
                     /locus_tag="ACL8A1_001723"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HGS1202451.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWETFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            12282..13742
                     /gene="gltX"
                     /locus_tag="ACL8A1_001724"
     CDS             12282..13742
                     /gene="gltX"
                     /locus_tag="ACL8A1_001724"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HGS1202452.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDKFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            13756..14190
                     /locus_tag="ACL8A1_001725"
     CDS             13756..14190
                     /locus_tag="ACL8A1_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HGS1202453.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAEDGQIFTGF
                     CMEGTCGVFHLCAERAALFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNER"
BASE COUNT         4178 a         2747 c         3189 g         4112 t
ORIGIN      
        1 ctcatgaaga tatgtcctca agcacaaact gcacgtaaat ccagcaaact caagccacta
       61 acagctgaag ataaagccta taaccatgcg ctatctaagg agagaagcaa ggttgagaac
      121 atctttgcca aagtaaaaac gtttaaaatg ttttcaacaa cctatcgaaa tcatcgtaaa
      181 cgcttcggat tacgaatgaa tttgattgct ggtattatca atcatgaact aggattctag
      241 ttttgcagga agtctattat ttggttaggt gaattagtga agtgtttagg caagtgtctc
      301 tggttacgac gtcatggcct ctaaatcgat tatatttagg ggtcatgact agtgaagcag
      361 ttagctagtt cgcatataag cggctagcgt ctaacaatta ggaactttag ttccaataac
      421 tttaagatta cgacgtttta ggacataaat cgatcatatt tatgtcctaa aactagtgaa
      481 gcgcctagcc aaagtccgaa taggatttgg cgttagttac ttagattgct ttgcaatcaa
      541 gtaactttgg cgatttacat cttctctggc gcttctactc caagcaagcg aagggcttct
      601 ttgagaacga ctgcggttgc gtagctgagg gctagacggc tgtcgcgttc tgggctttca
      661 tccaagatac gtgtatgtgc atagtatttg ttaaaggatt gagccaggct aattgcaaat
      721 ttagcaatga tagaaggttc aaagttatct gccgcacggt tgataatacg tgggaagtct
      781 tgaatgagtt taatgatttc ccagctttca gtatcattca agctatagtt gccagctgtt
      841 tctggtttga aatcggcttt gcgtaagata gattggatac gagcgtaggc atattgaacg
      901 taaggtccag tttcaccctc gaaggatacc atagcttcta ggtcgaagtc gtatccattt
      961 gtacggtcgg ttttgaggtc atagaattta atggctccaa ccccaacagc atgtgctact
     1021 tggtctttgt tttctagttc aggattttta gcctcgattt ggaccttggc acggctaaca
     1081 gcctctgcaa cagtaggctc tagcaagatg acattccctt tacgagtaga gagcttcttc
     1141 ccttcttttg taaccagacc aaaaggaacg tgggtaatgt cgtcactcca atcgtagccc
     1201 atctcttgca agacagcttt gagctgttta aagtgggcag attgttcttg accaacgaca
     1261 tagatagatt tagcaaattg gtattcgttt ttacggtaga gggctgcagc caagtcacgt
     1321 gtgatataga gagttgcacc atcagacttc ttgatgaggg ctggatgttc aattccatat
     1381 ttctcaagat tcacaacttg ggcaccttct gattcaagaa gtagtccttt ttcagaaaga
     1441 atgtctacaa ctgcatccat cttatcattg tagaaggctt ctccgttata gctgtcaaat
     1501 tcaaccttca attcattgta aaggcggtta aattccacta aactttcatc gcggaaccat
     1561 tgccaaagag cgagagcttc ctcatctcca ttttcaagtt tacggaacca ttcgcgcgct
     1621 tcttcatcca agctagggtc attttcagct tcagcgttga tgcggacata gagtttaagg
     1681 agttcatcga ttggatgagc ttttacagct tcttcgtcgc cccatttttt gtaggcaaca
     1741 atcaacatcc caaattgttt accccagtct cccaaatggt tgaccttgac cgtttgataa
     1801 ccgatttttt ggaaaatatg tgacaagcta tctccgataa cagttgaacg caggtggcca
     1861 atagaaaatg gtttagcgat attcggacta gacatgtcga taacaacatt ttcttgttta
     1921 ccaatatttt ggtcagcata gtgttctttt tcagtggtaa cagcttgcaa tacttgagca
     1981 gaaatggcag atttatcaag gaaaaagtta acgtaaggtc ctgttgcgac aactttttca
     2041 aaggcttggc tgttcatttt ttcagccagt tcagccgcaa tcatttgtgg tgctttacgt
     2101 tcgacttttg caagagaaaa agcagggaaa gcaatgtctc ccatttctga gtttttaggg
     2161 gtttccagta actttaaaat agcctcttgg tccaggctat caatgatgct agataatttg
     2221 ctagcaatca attcttttgt attcattaag agctcctttt tggacttttc tactatttta
     2281 tcacaatttt aaagaaagaa gaaaaaattt ttgaaatctc ctgttttttt ggtataatat
     2341 ggttataaat atagttataa atatgcacgc aagaggattt tatgagaaaa agagatcgtc
     2401 atcagttaat aaaaaaaatg attactgagg agaaattaag tacacaaaaa gaaattcaag
     2461 atcggttgga ggcgcacaat gtttgtgtga cgcagacaac cttgtctcgt gatttgcgcg
     2521 aaatcggctt gaccaaggtc aagaaaaatg atatggtgta ttatgtacta gtaaatgaga
     2581 cagaaaagat tgatttggtg gaatttttgt ctcatcattt agaaggtgtt gcaagagcag
     2641 agtttacctt ggtgcttcat accaaattgg gagaagcctc tgttttggca aatattgtag
     2701 atgtaaacaa ggatgaatgg attttaggaa cagttgctgg tgccaatacc ttattggtta
     2761 tttgtcgaga tcagcacgtt gccaaactca tggaagatcg tttgctagat ttgatgaaag
     2821 ataagtaagg tcttgggagt tgctctcaag acttattttt gaaaaggaga gacagaaaat
     2881 ggcgatagaa aagctatcac ccggcatgca acagtatgtg gatattaaaa agcaatatcc
     2941 agatgctttt ttgctctttc ggatgggtga tttttatgaa ttattttatg aggatgcggt
     3001 caatgctgcg cagattctgg aaatttcctt aacgagtcgc aacaagaatg ccgacaatcc
     3061 gatccctatg gcgggtgttc cctatcattc tgcccaacag tatatcgatg tcttgattga
     3121 gcagggttat aaggtggcta tcgcagagca gatggaagat cctaaacaag cagttggggt
     3181 tgttaaacga gaggttgttc aggtcattac gccagggaca gtggtcgata gcagtaagcc
     3241 ggacagtcag aataattttt tggtttccat agaccgcgaa ggcaatcaat ttggcctagc
     3301 ttatatggat ttggtgacgg gtgactttta tgtgacaggt cttttggatt tcacgctggt
     3361 ttgtggggaa atccgtaacc tcaaggctcg agaagtggtg ttgggttatg acttgtctga
     3421 ggaagaagaa caaatcctca gccgccagat gaatctggta ctctcttatg aaaaagaaag
     3481 ctttgaagac cttcatttat tggatttgcg attggcaacg gtggagcaaa cggcatctag
     3541 taagctgctc cagtatgttc atcggactca gatgagggaa ttgaaccacc tcaaacctgt
     3601 tatccgctac gaaattaagg atttcttgca gatggattat gcgaccaagg ctagtctgga
     3661 tttggttgag aatgctcgct caggtaagaa acaaggcagt cttttctggc ttttggatga
     3721 aaccaaaacg gctatgggga tgcgtctctt gcgttcttgg attcatcgcc ccttgattga
     3781 taaggaacga atcgtccaac gtcaagaagt agtgcaggtc tttctcgacc atttctttga
     3841 gcgtagtgac ttgacagaca gtctcaaggg tgtttatgac attgagcgct tggctagtcg
     3901 tgtttctttt ggcaaaacca atccaaagga tctcttgcag ttggcgacta ccttgtctag
     3961 tgtgccacgg attcgtgcga ttttagaagg gatggagcaa cctactctag cctatctcat
     4021 cgcacaactg gatgcaatcc ctgagttgga gagtttgatt agcgcagcga ttgctcctga
     4081 agctcctcat gtgattacag atgggggaat tatccgaact ggatttgatg agactttaga
     4141 caagtatcgt tgcgttctca gagaagggac tagctggatt gctgagattg aggctaagga
     4201 gcgagaaaac tctggtatca gcacgctcaa gattgactac aataaaaagg atggctacta
     4261 ttttcatgtg accaattcgc aactaggaaa tgtgccagcc cactttttcc gcaaggcgac
     4321 gctgaaaaac tcagaacgct ttggaaccga agaattagcc cgtatcgagg gagatatgct
     4381 tgaggcgcgt gagaagtcag ccaacctcga atacgaaata tttatgcgca ttcgtgaaga
     4441 ggtcggcaag tacatccagc gtttacaagc tctagcccaa ggaattgcga cggttgatgt
     4501 cttacagagt ctggcggttg tggctgaaac ccagcatttg attcgacctg agtttggtga
     4561 cgattcacaa attgatatcc ggaaagggcg ccatgctgtc gttgaaaagg ctatgggggc
     4621 tcagacctat attccaaata cgattcagat ggcagaagat accagtattc aactggttac
     4681 agggccaaac atgagtggga agtctaccta tatgcgtcag ttagccatga cggcggttat
     4741 ggcccagctg ggttcctatg ttcctgctga aagcgcccat ttaccgattt ttgatgcgat
     4801 ttttacccgt atcggagcag cagatgactt ggtttcgggt cagtcaacct ttatggtgga
     4861 gatgatggag gccaataatg ccatttcgca tgcgaccaag aactctctca ttctctttga
     4921 tgaattggga cgtggaactg caacttatga cggaatggct cttgctcagt ccatcatcga
     4981 atatatccat gagcacatcg gagctaagac cctctttgcg acccactacc atgagttgac
     5041 tagtctggag tctagtttac aacacttggt caatgtccac gtggcaactt tggagcagga
     5101 tgggcaggtc accttccttc acaagattga accgggacca gctgataaat cctacggtat
     5161 ccatgttgcc aagattgctg gcttgccagc agacctttta gcaagggcgg ataagatttt
     5221 gactcagcta gagaatcaag gaacagagag tcctcctccc atgagacaaa ctagtgctgt
     5281 cactgaacag atttcactct ttgatagggc agaagagcat cctatcctag cagaattagc
     5341 taaactggat gtgtataata tgacacctat gcaggttatg aatgtcttag tagagttaaa
     5401 acagaaacta taaaaccaag actcactagt taatctagct gtatcaagga gacttctttg
     5461 acaattctcc acttttttgc tagaataaca tcacacaaac agaatgaaaa ggagctgacg
     5521 cattgtcgct cccttttgtc tattttttaa ggagaaagta tgctgattca gaaaataaaa
     5581 acctacaagt ggcaggcctt ggcttcgctc ctgatgacag gcttgatggt tgctagttca
     5641 cttctgcaac cgcgttatct gcaggaagta ttagacgccc tccttactgg gaaatatgaa
     5701 gctatttata gtatcggggc ttggttgatt ggtgtggccg tagtcggtct agttgctggt
     5761 ggactcaatg ttgtcctcgc agcctatatt gcccaaggag tttcatccga ccttcgggag
     5821 gatgccttcc gtaaaattca aaccttttct tatgctgata ttgaacaatt taatgcggga
     5881 aatctagtcg ttcgaatgac aaatgatatc aaccagattc agaacgttgt catgatgacc
     5941 ttccaaattc ttttcagact tcccctcttg ttcatcggtt cgtttatcct agcggttcaa
     6001 accttacctt ctctgtggtg ggtgattgtt ctcatggtag tcttgatttt tggtttgact
     6061 gctgtcatga tgggaatgat ggggcctcgt tttgctaaat ttcaaaccct tcttgagcgc
     6121 atcaatgcca ttgctaagga aaatttacgt ggcgttcgtg tggtcaagtc ctttgtccaa
     6181 gaaaaagagc aatttgctaa gtttacagag gtctcagacg agcttcttgg tcaaaacctt
     6241 tacattggtt atgccttttc agtagtggaa ccctttatga tgttggttgg ttacggggcg
     6301 gtcttcctct ctatttggct ggtcgcggga atggttcagt cggatccgtc tgttgttggt
     6361 tccatcgctt cttttgttaa ttacctaagc cagattatct ttaccattgt tatggttgga
     6421 tttttgggaa attctgtcag ccgtgccatg atttccatgc gtcgtattcg agaaattctt
     6481 gacgcagagc cagctatgac cttcaaggat atcccagatg aagagttggt tggaagtctt
     6541 agctttgaaa atgtgacctt tacctatcca atggacaagg aaccgatgct gaaagatgtg
     6601 agctttacta ttgaacctgg tcaaatggtt ggtgtagttg gagcgactgg tgcaggaaag
     6661 tcaaccttgg ctcaattgat tccacgtctc tttgatccac aggacggggc cattaaaatc
     6721 ggtggcaagg atattcgaga agtgagtgaa ggaaccctgc gtaaaacagt ttccatcgtt
     6781 ctccaacgtg ccattctttt tagtggaacg attgcagata acttgagaca ggggaagggg
     6841 aatgctactc tatttgaaat ggagcgcgca gccaatattg cccaggctag tgaattcatt
     6901 catcgtatgg agaaaacctt tgaaagtcca gttgaagaac ggggaaccaa tttctctggt
     6961 ggacaaaaac aaaggatgtc gattgcgcgt gggattgtca gcaatccacg tattctgatt
     7021 tttgatgatt cgacctcagc cttggatgcc aaatcagagc gcttggtgca agaagctttg
     7081 aataaggact tgaaggggac gacaaccatt attattgctc aaaaaattag ctcggttgtc
     7141 catgcagaca agatcttggt tctaaatcaa ggacgattga ttggtcaagg cacgcatgca
     7201 gacttggttg ccaacaatgc cgtttaccgt gaaatctatg aaacacagaa atgaaagaca
     7261 aactataaga aaagtcaata gttttatcta aactatttct tatttcaatt tgatgatttg
     7321 gcgatgattt tagagcacgg caaaaagcta ttgaaaaagt ccattttttc aaaggtaatc
     7381 ctgtgttaat ttcagaaatt atatcacttt ttgttcgtca aatggcagct cttttttagg
     7441 atataaaaca gggttcggat aagttttttt gcaaggtgga tgatggctac attgtaatgt
     7501 tttccttatt ctaacttagt cttaagatag gccttaaaag caggtgaaaa gcgagggcat
     7561 gctttggcag cttgtatgag tacctaccgc agatgagggg aaccccgttt gaccattctt
     7621 cctgctaaat caatctgacc tgactgataa atagaagaat ccagtccagc gaaagcttgt
     7681 aattgagcag gattatcaaa ggcatgaata tttcgaatct cggctaaaat gaccgcccct
     7741 aaacgattct caatcccagt aaccgtcgtg atgaccgagt tgaactcaga catcaagtca
     7801 ttgacacatt ttttcgcctt gtcaatgagc ctcttgtaat gtttgatgat ttcattacac
     7861 gagataaaac gtctatgcgt tatcaaactc attaccaatt aaaacaaaaa gctgtggtta
     7921 gatcctttcg gaaatcgtca agcgattgga ggaaatgaac taatccacag cggcttattc
     7981 caagtatacc acttgggctt tggcagtagc taactgcgct aaatataata taaggaggag
     8041 taaaatgaag acagttcaat ttttttggca ttattttaag gtctacaagt tctcatttgt
     8101 agttgtcatc ctgatgattg ttctggcgac ttttgcccaa gccctctttc cagtcttttc
     8161 tggacaagcg gtgacgcagc tagccaattt agttcaagct tatcaaaatg gcaatccaga
     8221 acttgtatgg caaagcctat caggaatcat ggtcaatctt ggcctgctgg ttttggttct
     8281 atttatctct agtgtaatat acatgtgtct catgacgcac gtgattgcag aatcgaccaa
     8341 cgagatgcgc aaaggcctct ttggtaagct tgctcagttg acggtttctt tctttgaccg
     8401 tcgacaagat ggcgatatcc tgtctcattt taccagtgat ttggataata tcctccaagc
     8461 ctttaacgaa agcttgattc aggtcatgag caatattgtt ttatacattg gtctgattct
     8521 tgtcatgttt tcgagaaatg tgacgctggc tctcatcacc attgccagca ccccattggc
     8581 tttccttatg ctgattttca tcgtgaaaat ggcacgcaaa tacaccaacc tccagcagaa
     8641 agaggtaggg aagctcaacg cctatatgga tgagagcatc tcaggccaaa aagccgtgat
     8701 tgtgcaagga attcaagagg atatgatggc aggatttctt gaacaaaatg agcgcgtgcg
     8761 caaggcaacc tttaaaggaa gaatgttctc aggaattctt ttccctgtca tgaatgggat
     8821 gagcctgatt aatacagcca tcgtcatctt tgctggttcg gctgtacttt tgaatgataa
     8881 gtctattgaa acaagtacag ccctaggttt gattgttatg tttgcacaat tttcacagca
     8941 gtactaccag cctattatcc aagttgcagc gagttgggga agccttcagt tggcctttac
     9001 tggagctgaa cgaattcagg aaatgtttga tgcagaggag gaaatccgac ctgaaaaggc
     9061 tccaaccttc actaagttgc aagaaagtgt tgaaatcagt catatcgatt tttcatactt
     9121 gcctgataaa cctattttga aagatgtcag catttctgcc cctaaaggcc agatgacagc
     9181 agttgttggg ccgacaggtt caggaaaaac gactattatg aacctcatca atcgctttta
     9241 tgatgttgat gctggtggta tttattttga tggtaaagac attcgtggct atgacttaga
     9301 tagtcttaga agcaaggtgg gaattgtatt gcaagattcg gtcttgttta gcggaacgat
     9361 tcgagacaat atccgatttg gtgtgccaga tgctagtcag gaaatggttg aggtagcagc
     9421 aaaagcaacc cacattcacg actatatcga aagtttgcct gataagtacg atactcttat
     9481 tgatgatgac cagagcatct tttcaacagg gcagaagcaa ttgatttcaa tcgctcgaac
     9541 cctgatgaca gatccagaag ttctcattct cgatgaagca acttcaaacg tagatacggt
     9601 gacagaaagc aagattcagc atgccatgga ggtggttgta gcaggtagaa ctagtttcgt
     9661 cattgcccac cgcttgaaaa ccattctcaa tgcagatcag attattgtcc ttaaagatgg
     9721 agaagtcatt gaacgtggta accaccatga acttttgaag ctaggtggct tttattcaga
     9781 actctatcac aatcaatttg ttttcgaata agaaagaagt tgtcctatgt gggcagcttt
     9841 ttcttgtcca taaaaaatgt ttatcacagc cttaaaaaaa acatattaga cgaaagtcat
     9901 tttgagtgat atgataggac tatcgttagc attcgaaagg agaggcatca tggctagaac
     9961 ggttgtagga gttactgcaa atctatgtcc cgtagacgca gaaggcaaaa tcattcattc
    10021 atctgtatct tgtagattcg cagagatcat tcgtcaagtc ggtggtctcc ctttagtcat
    10081 tcctgttggt gatgagtcag ttgtacgtga ttatgtggaa atgattgaca aactcatttt
    10141 gacaggaggc caaaatgttc atcctcagtt ttatggagag aaaaagaccg tcgagagcga
    10201 tgattacaat ctggtccgtg acgaatttga attggcactc ttgaaggaag cgcttcgtca
    10261 gaataaacca attatggcaa tctgtcgcgg tgtccaactt gtcaatgttg cctttggtgg
    10321 aaccctcaat caagaaatcg aaggtcactg gcaaggacta cctttcggga catctcactc
    10381 tattgagaca gtagaaggaa gcgtggtggc taagctattt gggaaagaaa gtcaggtcaa
    10441 ttcagtccat cgtcaaagca ttaaagattt ggcacccaat ttccgtgtaa ctgctattga
    10501 ttcaagagac caaactattg aagcgattga gtctatcgac gagcaccgca ttatcggttt
    10561 acagtggcat ccagagtttc tggttaatga agaagatggc aatttagaat tatttgagta
    10621 tttattgaat gaactgtaac gattggaaca tctggtcgtt ctttctttta ttttttcaaa
    10681 atttttagaa tgtggtattt tacgcaaacg tttgaattct gataaaaatt ggagaaattc
    10741 gacaaaaaaa cttgaaaaaa acgaaggtaa gcgttatgat agaaaagaag aaatattgga
    10801 ggaagaacat gtcacatatt aaatttgatt attcaaaagt tttagacaaa tttgttgcac
    10861 cacatgaagt ggaatacatg caatcacaag taacagcagc agatgaattg atccgtaaag
    10921 gaactggtgc tggtagcgac ttcttgggat ggttggacct tcctgaaaaa tacgaccgcg
    10981 aagaatttga ccgcatcttg aaagctgctg agcaaatcaa gtcagacagc gatgttttgg
    11041 ttgtgatcgg tattggtgga tcttaccttg gtgcaaaagc agcaatcgac ttcttgaatc
    11101 accattttgc taacttgcaa acaaaagaag aacgcaaagc tccacaaatc ctttacgcag
    11161 gaaactcaat ctcatctact taccttgctg acttggtaga gtatgttgca gacaaagact
    11221 tctcagtaaa cgtaatttct aaatcaggta caacaactga accagcgatt gctttccgtg
    11281 tctttaaaga actcttggtt aagaaatacg gtcaagaaga agctaacaaa cgtatctatg
    11341 caacaactga ccgccaaaag ggtgctgtta aggttgaagc agacgctaac ggttgggaaa
    11401 catttgttgt tccagatgat atcggtggac gcttctcagt attgacagcc gttggtttgc
    11461 ttccaatcgc agcatcagga gctgacatca aagctcttat ggaaggtgcg aatgcagctc
    11521 gcaaagacta cacttcagac aaaatctctg aaaacgaagc ttaccaatac gcagctgttc
    11581 gtaacatcct ttatcgtaaa ggctatgcaa ctgagatctt ggtaaactat gagccatcac
    11641 ttcaatactt ctcagaatgg tggaaacaat tggctggtga atcagaagga aaagaccaaa
    11701 aaggtatcta cccaacttca gccaacttct caactgactt gcactcactt ggtcaattta
    11761 tccaagaagg aactcgtatc atgtttgaaa cagttgtccg tgttgacaaa cctcgtaaaa
    11821 acgtgcttat tcctactttg gaagaagacc ttgacggact tggttacctt caaggaaaag
    11881 acgttgactt tgtaaacaaa aaagcaactg acggtgttct tcttgcccac acagatggtg
    11941 atgtaccaaa catgtatgtg actcttccag agcaagacgc tttcactctt ggttacacta
    12001 tctacttctt cgaattggca attgcccttt caggttactt gaatgctatc aacccatttg
    12061 accaaccagg tgttgaagct tataaacgta acatgtttgc ccttcttgga aaaccaggat
    12121 ttgaagaatt gagcaaagaa cttaacgcac gtctataata gaagaaaaga gtggtttacc
    12181 cactcttttt actctcttta tccatagaaa ttggactcag tcaagacttg tgatataata
    12241 tagaaagcaa aaaggcagat gcctagataa taggagaaac tatgtcaaaa gatatccgcg
    12301 tacgttacgc accaagtcca acaggactac tacacatcgg aaatgctcgt acagcattgt
    12361 ttaattactt gtatgcgcgc catcatggtg gaacatttct catccgtatc gaagatactg
    12421 accgtaaacg ccatgtcgag gatggtgaac gttcacaact tgaaaacctt cgctggttag
    12481 gcatggattg ggatgaaagt ccagaatcac atgagaatta tcgccagtct gagcgtttag
    12541 acttgtatca aaaatacatt gaccaactat tagctgaagg aaaagcctat aaatcttacg
    12601 ttacagaaga agagttggca gctgaacgcg aacgccaaga agtagctggc gaaacaccac
    12661 gctacatcaa tgaatacctt ggtatgagtg aagaagaaaa agcagcttac atcgcagaac
    12721 gtgaagcagc agggatcatc ccaactgttc gtttggctgt caatgagtca ggtatctaca
    12781 agtggcatga tatggtcaaa ggcgatatcg aatttgaagg tggcaatatc ggtggtgact
    12841 gggttatcca aaagaaagac ggttacccaa cttacaactt tgccgttgtt atcgatgacc
    12901 acgatatgca aatctctcat gttatccgtg gagatgacca tattgctaat acaccaaaac
    12961 agcttatggt ctatgaagct cttggttggg aagctccaga gttcggtcac atgaccttga
    13021 ttatcaactc tgagactggt aagaagttat ctaaacgtga tactaataca cttcaattta
    13081 tcgaagatta ccgaaagaaa ggttaccttc cagaagcagt ctttaacttt attgctcttc
    13141 ttggttggaa cccaggtggt gaagatgaga ttttctctcg tgaagaactc attaaacttt
    13201 ttgatgaaaa ccgcctcagc aagtcaccag cagcctttga tcagaaaaaa ctagactgga
    13261 tgagcaatga ttatatcaag aatgcagacc ttgaaaccat ctttgaaatg gcaaaaccat
    13321 tcttagagga agcaggccgt ttgactgaca aggctgaaaa attagttgag ctctataaac
    13381 cacaaatgaa atcagtagat gagattatcc cattgacaga tcttttcttc tcagatttcc
    13441 cagaattgac agaagcagag cgcgaagtca tgacgggtga aacagttcca acagttcttg
    13501 aagcattcaa agcaaaactt gaagcgatga cagatgataa atttgtgaca gaaaatatct
    13561 tcccacaaat taaagcagtt caaaaagaaa caggtattaa agggaaaaat cttttcatgc
    13621 ctattcgtat cgcagtttca ggcgaaatgc atgggccaga attaccagat acaattttct
    13681 tgcttggacg tgaaaaatca attcagcata tcgaaaacat gctaaaagaa atctctaaat
    13741 aaagaggata caataatgga catctgggaa aagatgtacg aagaagcaca gaaactatac
    13801 aatccacatg aagtatcaga ctttgtttat gccaatcatg ttgtagctgc agtagaagca
    13861 gaagatggac aaatatttac aggattctgt atggagggaa cctgtggtgt tttccatctc
    13921 tgcgcagaac gggcggccct cttcaatatg taccaatttt caggacaaac taaggttaag
    13981 aaagtattag cctttcgaga caaaccacct tatggtggaa gttcagccat gccttgcggt
    14041 gcttgtagag aattcctttt agagcttaat gctgaaaata aagatgcaga attcatgatg
    14101 gactataata taagaaaaac agttaaagtc gcagaactaa tcccttattg gtggggagaa
    14161 gaacgtgctt ctaagtttaa tgagcgatag aagagtcagt ataattctgg ctttttttac
    14221 tataag
//
LOCUS       DBERMR010000051        14040 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.051, whole genome shotgun sequence.
ACCESSION   DBERMR010000051 DBERMR010000000
VERSION     DBERMR010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 14040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14040
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.051"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            84..881
                     /locus_tag="ACL8A1_001726"
     CDS             84..881
                     /locus_tag="ACL8A1_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HGS1202454.1"
                     /translation="MISSEFISKIEFACNKKEGLYSQSKFKYAIRSIFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLIHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            1029..2288
                     /locus_tag="ACL8A1_001727"
                     /pseudo
     CDS             1029..2288
                     /locus_tag="ACL8A1_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365067.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
     gene            2384..3658
                     /locus_tag="ACL8A1_001728"
     CDS             2384..3658
                     /locus_tag="ACL8A1_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HGS1202455.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMKQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHEALLAKDKEV
                     QLLENQLATLRLEHENQLQKTLSDLEKERDQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3668..4546
                     /gene="truB"
                     /locus_tag="ACL8A1_001729"
     CDS             3668..4546
                     /gene="truB"
                     /locus_tag="ACL8A1_001729"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HGS1202456.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEIILGYSTTTEDASGEVVAETPVLSSLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            4787..5089
                     /locus_tag="ACL8A1_001730"
     CDS             4787..5089
                     /locus_tag="ACL8A1_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HGS1202457.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQS
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            5197..5835
                     /gene="udk"
                     /locus_tag="ACL8A1_001731"
     CDS             5197..5835
                     /gene="udk"
                     /locus_tag="ACL8A1_001731"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HGS1202458.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            5963..7303
                     /gene="xseA"
                     /locus_tag="ACL8A1_001732"
     CDS             5963..7303
                     /gene="xseA"
                     /locus_tag="ACL8A1_001732"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HGS1202459.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESIVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            7281..7493
                     /locus_tag="ACL8A1_001733"
     CDS             7281..7493
                     /locus_tag="ACL8A1_001733"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HGS1202460.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            7490..8365
                     /locus_tag="ACL8A1_001734"
     CDS             7490..8365
                     /locus_tag="ACL8A1_001734"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HGS1202461.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVAIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            8358..9173
                     /locus_tag="ACL8A1_001735"
     CDS             8358..9173
                     /locus_tag="ACL8A1_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HGS1202462.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVYGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKVHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            9166..9597
                     /locus_tag="ACL8A1_001736"
     CDS             9166..9597
                     /locus_tag="ACL8A1_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HGS1202463.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            9604..11271
                     /gene="recN"
                     /locus_tag="ACL8A1_001737"
     CDS             9604..11271
                     /gene="recN"
                     /locus_tag="ACL8A1_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HGS1202464.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            11273..12001
                     /locus_tag="ACL8A1_001738"
     CDS             11273..12001
                     /locus_tag="ACL8A1_001738"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HGS1202465.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQTQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEAADLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            12080..13903
                     /gene="lepA"
                     /locus_tag="ACL8A1_001739"
     CDS             12080..13903
                     /gene="lepA"
                     /locus_tag="ACL8A1_001739"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HGS1202466.1"
                     /translation="MNLEELKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
BASE COUNT         4152 a         2562 c         3350 g         3976 t
ORIGIN      
        1 aacttttatg aaatacagaa aacgcttcct aaaacctaaa gtttgtgata taattatcaa
       61 gaaaaaagat ttaggagttt attatgattt cttcagaatt tatctcaaag attgaatttg
      121 cttgcaataa gaaagaaggt ctttatagtc aaagcaaatt taagtatgcg attcgttcga
      181 tattcgcagg tgcattttta accttcagta ctgctgcagg tgcagttggg gctgacttga
      241 ttaataaaat tgcaccaggt agtggacgct tcctctttcc attcgttttt gcttggggct
      301 tggcctacat tgtttttttg aatgccgagt tggtcacttc aaacatgatg ttcttgactg
      361 ctggtagttt cttaaaaaaa atctcttgga gaaaaacagc tgagatttta ctatactgta
      421 ccttgttcaa ccttatcgga gccttgatag cagggtgggg ctttgctcat tcggcagcct
      481 atgcgaatct gatacacgat agtttcatct caggtgttgt tgagatgaag ttaggccgct
      541 caaatgaatt ggtcttgctt gaggcgattt tggcaaatat tttcgtaaat attgcgattc
      601 tgtcatttat tttggtcaaa gatggtggtg ccaaactttg gcttgtgttg tcagctattt
      661 acatgtttgt attcttaaca aacgagcaca ttgcggcgaa ctttgcttct ttcgcgattg
      721 tgaaattcag tgttgctgcg gattcaattg ccaacttcgg tgttggaaat atgcttcgcc
      781 actggggtgt gactttcatc ggaaacttta tcggaggagg cctcttgatg ggtcttccat
      841 atgccttcct caataaaaac gaagatactt atgtagatta agaaaatgag cacgattgag
      901 tcgtgctttt ttcattttca aaataaggta atagctattt cttatatcaa aatatagaaa
      961 actgatattt gtaaactata actcaaggtg ctacaatatc cttaataaaa tgatatggag
     1021 gtcaccttat gacttgtgat tttaaatttg aaactctaca actacatgct ggtcaagttg
     1081 tggctccagc tactaagtct cgtgcagtgc cgatttatca aacaacatct tttatttttg
     1141 atgacacgta ggaaggtgct gatctgtttg ccttgaggaa aacagggaac atttatactc
     1201 gtatcaccaa tcctacaaca gctgcccttg aaggtggtgt tgaagcgcta gcaacagcat
     1261 caggtatgac tgcagtgact tatactattt tggcgattgc ccatgctggt gaccatgtag
     1321 tggctgcttc gactatttac ggtggaacct tcaatctttt gaaagaaccc cttcctcgtt
     1381 atggtatcac aacaaccttt ttcgatattg ataatttgga ggaagtagaa gcagctatca
     1441 aagacaatac caagcttgtc ttgattgaaa ccttgggtaa ccccttgatt aatattccag
     1501 acctggaaaa actggcagag attgctcata aacatcaaat cccacttgtg tcagacaata
     1561 cttttgcaac accttatttg attaacgtct tctctcatgg cgttgacatt gccattcact
     1621 ctgtgactaa gtttatcggt gggcatggta caactattgg aggaataatt gtcgatagtg
     1681 gtcgttttga ctggacggct tcagggaaat tccctcaatt tgttgacgag ggtccaagct
     1741 gccacaattt gagctatact cgtgatgtgg gtgcagcagc ctttattata gctgttcgag
     1801 ttcaattgct tcgtgataca ggtgcagcct tgtcaccatt caatgctttc ctcttgctac
     1861 aaagacttga aacctcttca cttcgtgtgg aacgccatgt acaaaatgct gagacaattg
     1921 ttgattttct tgtcaaccat cctaaggtag aaaaggtaaa ttatccaaaa cttgcagata
     1981 gtccttatca tgccttggct gagaaatact tgccaaaagg tgtcggttca atctttacct
     2041 tccacgtcaa aggtggcgag gaagaagcac gcaaggtcat tgataattta gaaatctttt
     2101 ctgaccttgc aaacgcggca gatgctaaat cgcttgttgt ccatccagca acaaccactc
     2161 acggtcaatt gtcagaaaaa gacctagaag cagcaggtgt cacaccaaac taaattcgtt
     2221 tgtcaatcgg tcttgaaaat gtagaagatt tgattgaaga cttgcgcttg gccttggaaa
     2281 aaatttaaag taaaagaaga taaacagtgg gcttcgactc actgtttttg attttccctc
     2341 aggcatgata taatggttaa agaagtctag aaagaggaac gatatgaacg aaatcaaatg
     2401 tcccaactgt ggggaagtct ttacagtaaa tgagagtcag tatgctgaac ttttgtccca
     2461 agtgagaacg gcagagtttg ataaggaact acacgatagg atgaagcagg aactggcctt
     2521 ggctgagcaa aaggccatga atgagcaaca gactaaactg gctcagaagg atcaagaaat
     2581 tgcgcaatta cagagtcaga tccaaaactt tgatacagaa aaagaattgg ccaagaaaga
     2641 ggttgaacag acaagccatg aggctctctt ggctaaggac aaggaagtac agctcttaga
     2701 aaatcagttg gctaccttgc gtttggagca tgaaaatcaa ctacaaaaga ccctttctga
     2761 cctagaaaaa gaacgggatc aggttaaaaa ccaactactt ttgcaggaaa aggaaaatga
     2821 attatctttg gcttctgtta agcaaaacta cgaagcccag ctcaaggcag ctagtgaaca
     2881 agtcgagttt tataagaatt ttaaggctca acaatctaca aaagcgattg gggaaagcct
     2941 agaacagtat gcagagagtg agtttaacaa ggttcgtagt ttcgcctttc caaatgctta
     3001 ctttgagaag gataacaagg tctcttcgcg tgggtctaaa ggggacttta tcttccgtga
     3061 gtgtgatgaa aatggagttg aaatcatttc tatcatgttt gagatgaaaa acgaagcgga
     3121 cggaacagag aagaagcaca agaatgcaga tttttacaag gaattggaca aggaccgtcg
     3181 ggagaagaac tgtgagtatg ccgttttggt gaccatgctt gaggctgata atgactactt
     3241 taacacaggg attgttgacg tcagtcacga gtatgaaaaa atgtatgttg ttcgtcctca
     3301 attctttatc caattgattg gtctcttacg taatgcggcg ctaaattccc taaaatacaa
     3361 gcaggagttg gccttggttc gcgagcaaaa tattgacatt acgcattttg aggaagattt
     3421 ggatgccttt aagctagctt ttgctaagaa ctataattca gcttcgacta actttggaaa
     3481 agctattgat gaaatcgaca aggccatcaa acgcatggaa gaggttaaga aattcctgac
     3541 cacatctgaa aaccaactcc gtttagctaa caacaaattg gaagatgtct ctgttaaaaa
     3601 attgacccgg aaaaatccaa caatgaaagc gaagttcgaa gcactgaagg gggagtagaa
     3661 agcaaaaatg aacggtatta tcaacttaaa aaaagaagca ggaatgacct cgcatgatgc
     3721 ggtttttaaa ctgcgtaaga ttttgggaac caagaaaatt ggtcatggtg gaaccttgga
     3781 tccggatgtg gtgggtgttt tgccgattgc ggttggcaag gcgacacgca tggtcgagtt
     3841 tatgcaggac gagggtaaga tctatgaggg ggaaatcatt ctgggctatt ccacgacgac
     3901 tgaggatgct agtggggaag tggtcgcaga aacccctgtt ttgtcttcat tggatgaaaa
     3961 gcttgttgat gaagcgattg ctagcttgac tgggcctatt actcagattc cccctatgta
     4021 ttcggcagtt aaggttaatg gtcgcaagct ctatgagtat gcgcgtgctg gtcaggaagt
     4081 ggagcgtcca gaacgtcagg tgaccattta tcaatttgaa cgaacaagtc cgatttctta
     4141 tgatggccaa cttgcccgat tcacttttcg tgtaaaatgc agtaaaggga cttacatccg
     4201 tactttgtca gttgatttgg gtgaaaagct tggttatgcg gctcatatgt cccatttgac
     4261 tcgtactagt gctgctggct tacaattaga agacgctctt gccttggagg aaattgctga
     4321 aaaagtagag gctgggcaat tagattttct ccatccttta gagattggga caggtgacct
     4381 tgtcaaagtt ttcctaactc cagaagaggc tacagaagtt cgctttggtc gttttattga
     4441 gctagaccaa acggacaaag aactggctgc ctttgaagat gataaattgt tagccattct
     4501 agaaaaacgg ggcaatctct ataagccaag gaaggttttt agctagatcg tttaggaata
     4561 aaaatcgggt gatagataac aattgcttga taaaacccca tactaatagt agaatggttt
     4621 tgggaattat aatattccaa ttgttgcgag ttgtaggtac tcaaataatc tatatagaaa
     4681 tttagaggtg tgaaatgaag caatttaaaa ttctttcaga taaatattta gagtccatta
     4741 caggttctgg tgggaactta ggcccaggat ttggtgtgat aattccatga tgcgaaatga
     4801 gtttcgagaa agggtggagc aacttcttca acaaaaagaa ataaatgaaa atagtgagtt
     4861 gagtcacctg tttcgtcttg ctatacaaaa tttagacaga aatgaaaaat accaatcggt
     4921 catggccaat ttgagtcaag ggttgtcact ttacctcatg acgcatcatt accaggcacc
     4981 taagtctgtc attgattttg gtttatggat tgccaaagct cctagccagg aaagagggag
     5041 actggctttc ttgcagatgc ttgctcaaac cctacagggc tttcgttaaa actgtcgaac
     5101 atcagaggga agactctggt gttttttgct gatgagacta taaattttgt aaaaaatgtg
     5161 atagaataga cgacggataa aataacggag gatagcatgc aaaatagacc aatcattatt
     5221 ggagtgacag gtggttctgg tggtggtaaa accagtgttt caagagccat tttatcgcat
     5281 tttcctgatg aaaagatttc catgattgag catgattcat actacaagga tcagtctcat
     5341 ttgacctttg aagagcgtgt caaaaccaac tatgaccatc cttttgcctt tgatacagac
     5401 ttgatgatcg agcagattaa ggaattgttg gcagggcgtc cggtggacat cccgacctac
     5461 gactatacag agcatacacg gagtagcaag acctatcgtc aggagcctca agatgtcttt
     5521 atcgttgagg gcattttggt cttggaggac aagcgtctgc gcgatttgat ggatatcaag
     5581 atttttgtgg atacggatga cgatgtgcgc attattcgtc gtatcaagcg tgatatggag
     5641 gagcgtggcc gtagccttga tagcgttatt aaccagtact taggtgtggt caaaccaatg
     5701 taccaccagt ttatcgagtc aactaagcgt tatgctgata tcgtcattcc tgaaggggtt
     5761 agcaataccg tggctatcga cctgttgacg accaagattg caaagatttt ggaagaagct
     5821 cgaaacagca aataatcaca tgtggaggcc ttgcctcctt tttctatttt tccctcataa
     5881 tggtacataa atggagattt ttaggcatat ttttggtata ataataccca taaaagagca
     5941 agtaaatgaa tagtaggtgg agatggaaaa gtatttatcg gtaacaactt tgaccaagta
     6001 tctgaaaatg aaattcgata aagacccata cttggaacgg gtctatttaa ctggtcaagt
     6061 ttccaacttt cgtaaacgac ctactcacca atatttctcc ctaaaggatg accatgcagt
     6121 tattcaagcg accatctggt ctgggattta tcagaaatta gggtttgacc tggaagaagg
     6181 aatgaagatc aatgtgattg ggcgtgtaca ggtctatgaa ccaagtggta gctactccat
     6241 catcattgaa aaagctgagc ctgatggggt tggggcgctt gcgattcagt ttgaacaact
     6301 taagaaaaaa ttgacagaag aaggcctgtt tcaagaacgc ttcaagcaag ctctacccca
     6361 attttctaag agaattgggg tagtaaccag ccgtagtgga gccgttattc gagatattat
     6421 cacgaccgtc agcaggcgat tcccaggtgt tgacatcctt ctttatccga ccaaggttca
     6481 aggtgaaggg gctgcggagg aaattgctag aaatattgcg cgtgctaatc aacgggatga
     6541 tttggatttg ctcattattg gtcgtggagg tggttctatt gaggatctct gggcctttaa
     6601 cgaagaaatt gtggtacgag ctatttttga atctcgtttg ccagttatct ctagtgtggg
     6661 gcatgagacg gatgtgacct tggcagattt tgtggcagat cgacgcgctg caacgccaac
     6721 agcggcggct gaactggcaa cacctgtgac caagttggat gtattagctc atttgcaaaa
     6781 tcaggaaaaa cggatggcaa cggcagtccg aaatgttcta tctaagaaac aagaggcttt
     6841 gaaaaaatgc agtcagtctg ttatctttag acaacctgag cgcttgtatg acggttattt
     6901 gcaacgcttg gaccaactgc aactgcgttt gaaacaaagt ttgcgaactc ggatttctga
     6961 taacaagcaa ttagttcaag caagaactca tcaattagta caattatcac ctgttaccaa
     7021 aatccaacgc tatcaagacc gtttaggaca gttggacaag ctcttaggta gccaaatggc
     7081 gttagtttat gacgccaagg ttgctgaggc caagcgactt tcggaagctt tgctcatgtt
     7141 ggatactagc cgaatcgtgg cgcgtggtta tgctattgtc aaaaaagaag agtccattgt
     7201 agattcggtt gagagtttga agaaaaaaga ccaagtaacg cttttgatgc gagatggtca
     7261 agtagaatta gaggttaaag atgtcaaaac aaaagaaatt tgaggaaaat ctagcagaac
     7321 tggaaaccat tgtccaaagt ttggaaaatg gtgaaattgc tctggaagat gcgattactg
     7381 cctttcaaaa gggcatggtc ttgtcaaaag agctccaagc tacgctggac aaggctgaaa
     7441 agaccttggt caaggtcatg caagaagacg gaacagaaag tgattttgaa tgaaaaagca
     7501 agaaaaatta gctcttgtcg agtcggcttt ggaagatttt tatggagacc agcagtttgc
     7561 ctctagttta cgggagtctg ttctctattc tattcatgct ggtggcaagc gtattcggcc
     7621 ttttctcttg ttagaagttc tggaagcctt gcaggttgcc atcaaacctg ctcacgcgca
     7681 ggtagctact gccttggaga tgattcatac agggagcttg attcacgatg accttcctgc
     7741 tatggatgat gacgattatc gaagagggcg gttaaccaat cacaagaaat tcggtgaagc
     7801 tatggccatt ttggctggag atgccttatt cttagaccca tatgccttga ttgcgcaggc
     7861 agatttgcca agtcagatca aggtggactt gattgccaac ttatcccttg cttcaggtag
     7921 tctgggtatg gtggcagggc aagttttgga tatggagggc gaacaccagc acttgtctct
     7981 ggaagaactt cagactattc atgccaataa gactgggaag ttactagcct atcccttcca
     8041 agcggcagct attatagctg aattgtcacc tgaaatgcag gtgaagctga aaactgtggg
     8101 tgaattgatt ggacttgctt ttcaagtcag agatgatgta ctggatgtga cagctagttt
     8161 tgaggaaatc ggcaagacac ctcaaaagga tctgcaggca gaaaaatcaa cctatcctgc
     8221 cttgttgggc ttggaagagt ccattgcctt ttgtaaccaa accctggatc aagctaatga
     8281 aaaattagaa gaaattgccc agcaacttcc ctttgaaaca gaatcgattg taagtgtagt
     8341 agaaagtttg agaatcaatg gctaaggaaa gagtggatgt actagcttat aaacaggggt
     8401 tgtttgaaac gagagagcag gccaagcgag gtgtgatggc tggcctagtc gtagcagtcc
     8461 ttaatggaga acggtttgac aagccaggag agaaaattcc agatgacacc gaattaaaac
     8521 tcaaggggga gaaactcaag tatgtcagcc gtggtggttt gaaactggaa aaggccttgc
     8581 aggtctttga tttgtcggtg tatggcgcga ctacgattga tatcggggcc tctactggag
     8641 gttttaccga tgtcatgcta cagaatagtg ccaagttggt ctttgcagtc gatgttggta
     8701 ccaatcagtt ggcttggaaa ttacgccaag acccacgagt tgtcagcatg gagcagttca
     8761 atttccgcta tgctgaaaag actgatttcg agcaggagcc gagctttgcc agtattgatg
     8821 tgagtttcat ttcccttagt ctgattttgc cagccttgca ccgtgtcttg gctgatcaag
     8881 gtcaggtggt agcacttgtc aaacctcagt ttgaggcagg acgtgagcag attgggaaaa
     8941 atggaattat tcgagatgct aaggttcatc agaatgtcct tgaatctgta acagctatgg
     9001 cagtagaggc aggtttttca gtccttggct tggacttttc tcccatccaa ggtggacatg
     9061 gaaatattga atttttagcg tatttgaaaa aagaaaagtc agcaagcaat cagattcttg
     9121 ctgagattaa agaagcagta gagagggcgc atagtcaatt taaaaatgaa taaaaaagag
     9181 agacttgaaa aaattagaag attggtgaca gattatcaaa tcggcacgca agaagaaatt
     9241 gtagaacatt tgaaagaagc aggtatcact gccactcagg cgacggtatc ccgagatatc
     9301 aaagagttag gtattgtcaa aattcctttg agagacaaca cctatgtcta cgaattgcca
     9361 aaatcaatcg taaaaagtct gcaactggct gaagacaata tcgaatcggc tgaattgatg
     9421 gataagatga tcaatctcca agttattcca ggaaatacgg cttttgtaaa agctcagtta
     9481 atcgagactt ttgcagacaa gatttttagc tgtttgactg atgatagctc gattttagtc
     9541 attgccagaa gtggaagtct agcagaggaa atctttgaac aagtaaaaaa ttggtaggtc
     9601 tatatgttac ttgaaatttc gataaaaaac tttgccatta ttgaggctat ttccctcaat
     9661 tttgaaaagg ggatgactgt cctgactggt gaaacgggtg cagggaagtc gattatcatt
     9721 gatgccatga atatgatgtt gggagctcgt gcgacgacag atgttattcg tcatggtgca
     9781 ccaaaggcag agattgaggg gcttttctca gttgagaata gtcgtctttt acaggaaatt
     9841 tttgatgagc aaggtttaga attaggtgat gaaattatca tccgtcggga aatcttgcaa
     9901 aatggtcgta gtatcagccg tgtaaatggt cagatggtta atctgtctgt tttgcgagct
     9961 attgggcaac atcttgtaga tattcatggt cagcatgacc atgaggagtt aatgcgtccc
    10021 caactgcata tccagatgtt ggatgaattt ggtgatgccg ctttttggga cttgaaagaa
    10081 acctatcaaa cgagttttga tgcctatcgg aaaatgcgca agcaggttct ggaagtcaag
    10141 aaaaaccaac aggaacacaa ggcacgtatc gaaatgttgg aatttcaaat ggcagagatt
    10201 gaggcagcaa acttgcaggc tggagaagac ttggctctca atcaagagcg agataaactc
    10261 ctcaaccaca aaaatattgc ggatacactg accaatgcct acagtatgtt ggacaatgaa
    10321 gatttttcaa gtctggccaa tgttcgttca gctatgaatg acatggaaag tgtcgaagag
    10381 tatgatcctg aataccgtga aatttcaagc tctctgtctg agacctacta tgttttagaa
    10441 gacattagca aacgtttgga agctatcatt gaggaccttg attttgatgg caatcgcctg
    10501 atgcaggttg agaatcgttt ggacctcctt catactatta ctcgtaagta tggtgggact
    10561 gttgatgatg ttttgcttta ttttgccaag attacggaag aatacaatct cttgacaggc
    10621 aataatcttt cgtctgagga catggaagca gagcttaaga agttggaagt caatcttgtc
    10681 aatttggcag gtcaacttgc ttctgctcgt cataatttgg ctcagcaact cgaagctgag
    10741 attaaacaag aactgcaaga tctttatatg gaaaaagccc agtttcaggt tcgttttagt
    10801 aagggaaaat tcagtcgtga gggaaatgaa atggttgagt tttatatttc aaccaaccct
    10861 ggagaagact ttaaaccctt ggttaaggtt gcttctggag gggaattatc tcgtctcatg
    10921 ttagccatta agtctgcctt ttcacgtaaa gaaggcaaga ctagcattgt ctttgatgag
    10981 gtggatacgg gagtttcagg tcgtgttgct caagctattg ctcagaaaat tcataaaatt
    11041 ggtcagcatg gtcaggtttt ggctatctcc catttgccac aagtaattgc gattgcagat
    11101 tatcaattct ttattgagaa gattagtaat gaccattcaa cggtttcgac tgttcgtctc
    11161 ttgacggtcg aagagcgagt ggaggaagtt gccaagatgt tggcaggtga tgatgtgaca
    11221 gaagcagccc tgacgcaagc cagagaattg ttgagaaaca gggagaaata agatgacaga
    11281 ttattatgta attggagatg ttcacggaaa agctgggatg ctggaagacc ttcttaaaac
    11341 ctgggatggt cagacccagt tgctctttct aggtgatttg attgacagag gtgaggatag
    11401 tcaccgtgtt cttgaaatgg tcaaggactt ggtggacaat caaggggcta tctgtttgtc
    11461 tggaaaccac gagtatatgt ttttgacttg gctagatgac ccagaagaaa gttatgacca
    11521 ttatcgtcgc aatggtggag atacaaccat taactctatc ctaggtcgtc ccttggatgc
    11581 accagttgat ggagtagaag atgccaagcg ggttgccgct gaagcggcag acttggtcga
    11641 attcattcgt caaatgccat ttgtagtaga gacagacaag tatatctttg ttcacgcagg
    11701 tattgatttg actttggacg actggcatga aacaacagat tataaaaaag tatggcttag
    11761 aaaaccattc cacggagctg aaaatcatac tggaaaaacc attgtcttcg gtcatacacc
    11821 ggtctatggt ttgttacagc aagaacgcgg tacatctgag ctttggacta cagaagatgg
    11881 caagattgga atggatggag gagctgttta tggtggtgtc cttcacggga ttgtctttac
    11941 agaccaagga atgacagaac accactttat cgaaaatgac ggctttgtcg ctgaagatta
    12001 gtactcctag cagggtatgg tcttgtcaaa atatcaaaaa caatttataa taaatagata
    12061 ccctgaaagg aagagaatca tgaacttaga agaattgaaa aaacgacagg agaagatccg
    12121 taacttctct attatcgccc atattgacca cggaaaatca actctagcag accgcatttt
    12181 ggaaaaaaca gagacggttt caagtcgtga aatgcaggcc cagcttttgg atagcatgga
    12241 tctagagcgg gaacgtggaa ttactattaa gttgaatgcc atcgagttga attacactgc
    12301 aaaagatggg gaaacttata ttttccactt gattgacaca ccagggcacg ttgactttac
    12361 ctatgaagtt tcacgttcgc tagctgcctg tgagggtgct attttggtgg tcgatgcggc
    12421 tcaaggaatt gaggctcaaa ctcttgccaa cgtttatctg gctttggata atgatttgga
    12481 aatcatgcct atcattaata aaattgacct gccggctgca gatccggagc gcgtgcgtac
    12541 agagattgaa gatgtgattg gtttggatgc cagtgaagca gttttggctt ctgccaaggc
    12601 tggtattggg attgaagaaa tcctcgagca aattgtagaa aaagtaccag caccaacggg
    12661 tgatgtgacg gcgccactta aggccttgat tttcgactct gtttacgatg cttaccgtgg
    12721 ggttatcctc caagtacgtg tcatggacgg agtggtcaaa cctggtgata agattcagct
    12781 catgagcaat agtaagacct ttgatgtggc cgaagtcggt atttttacac ccaaagcggt
    12841 tggtcgtgat ttccttgcga ctggtgatgt tggttacatt gcggcgtcta tcaagacggt
    12901 tcaggatact cgtgtgggtg ataccgttac cttggcaacc aatcctgcgg cagaaccatt
    12961 acatggttat aagcagatga atcctatggt ctttgcgggt ctctacccaa tcgaatcaaa
    13021 caagtacaat gacctgcgtg aagcgcttga aaaattgcaa ctgaatgatg ctagtcttca
    13081 gtttgaacca gaaacatctc aggcacttgg atttggtttc cgttgtggat ttcttggact
    13141 tctccatatg gatgttatcc aggaacgttt agagcgtgag ttcaatattg acctcatcat
    13201 gacagctccg tctgttattt acaaagttaa tttgaccgac ggtgagtcta tggatgtgtc
    13261 taacccatct gagtttccag acccaactaa gattgcgacc attgaagagc cttatgtcaa
    13321 ggcgcaaatc atggtaccac aggagttcgt cggagcagta atggagctag ctcagcgtaa
    13381 gcgtggggac tttgtgacta tggactatat tgatgataac cgtgtcaatg ttatctatca
    13441 aattcctctt gctgaaattg tctttgactt ctttgataaa cttaagtctt cgacacgtgg
    13501 ttatgcaagc tttgactacg aattgtcaga atatcgccca tctaagctgg tgaaaatgga
    13561 tatccttctc aatggagata aggtggatgc cctcagcttt atcgttcaca aggactttgc
    13621 ctacgaacgt gggaaactca tcgttgataa actcaagaaa atcatccctc gccaacaatt
    13681 tgaagttcca atccaagcgg ctattggaca caagattgtc gctcgtactg atatcaaggc
    13741 ccttcgtaag aacgtacttg ctaaatgtta tggtggtgac gtttctcgta agcgtaaact
    13801 ccttgaaaaa caaaaagctg gtaagaaacg catgaaatcc atcggatcag ttgaagttcc
    13861 gcaagaagcc ttcctcagcg tcttgagtat ggatgaagaa taagttatca gaaatgcctt
    13921 tccttgttca caggaaattt atataagaat caatagtaaa gcaggcttag atggtctgct
    13981 tttttagttt ttatactctt cgaaaatctc ttcaaaccac gtcagcgtcg ccttaccgta
//
LOCUS       DBERMR010000052        13585 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.052, whole genome shotgun sequence.
ACCESSION   DBERMR010000052 DBERMR010000000
VERSION     DBERMR010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13585
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.052"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            43..1188
                     /locus_tag="ACL8A1_001740"
     CDS             43..1188
                     /locus_tag="ACL8A1_001740"
                     /inference="COORDINATES: protein motif:HMM:NF025665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HGS1202467.1"
                     /translation="MSKKEIKPICFYLPQFHPIPENDEAHGEGFTEWTNVKKAYPLYD
                     GHNQPRVPYNNNYYSLLDKEVLIQQAELAKKYGIYGFCYYHYWFKNGKKLLEKPVEMM
                     LENKDVDIPFCLSWANENWTRRWDGGNHEIIVEQNYDDLNDLEKHVDYLCQFFKDDRY
                     IKIDGKPLLLIYKPELIPNLVKTMEIIRNRARKNGFKDLEIIVQFPKFLLNNHQLELF
                     DGFAEFQPIFEIHMEKHERKNPVYRSVKSFLLKVGARNLVQKLEIRGEKKVHNKALLD
                     RRDYDATWTNIINRPIKDNKMIAGAFVDWDNTARNKNGRVFDGANPEKFEGYMRQLIE
                     KIQKEYQSEIVFINAWNEWAEGAYLEPDKKHGYGYLEALKTVIDETR"
     gene            1197..1811
                     /locus_tag="ACL8A1_001741"
     CDS             1197..1811
                     /locus_tag="ACL8A1_001741"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HGS1202468.1"
                     /translation="MNIRYKLQFRRCKKEWRKKNLHNETTPRTMFPIELVSVGEKTYG
                     GLYVLSFNKESKLKIGNFCSIAPEVAFILSADHYINQFSTYPFKSKVFDQGDEGVSKG
                     DIIIEDDVWIGFRSTILSGVTIGQGAIVGAGSVVTKSVPPYAIVGGVPAKVISYRFET
                     EIREEMKKIDFSEFKLEKFKKVTEELYQPISSIRQINNINEKLK"
     gene            1878..3020
                     /locus_tag="ACL8A1_001742"
     CDS             1878..3020
                     /locus_tag="ACL8A1_001742"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HGS1202469.1"
                     /translation="MFIAMLIYCQPEYLKSIPVTGRIYRDFMHVGIMFVTFSYISKKR
                     FPKFFVWLVLLYNSWLTFLTIQNNGDIKRAIFQLLLFTGFVSMIDIFSENMQDLIESL
                     LFTFEMVVYLNFVTVLSFPDGFFHRVNDAYGITKEWFLGADNNFLFTLFPATLIALLY
                     KNLGGVKIRAYSLISIILATQLIKGSGTAKVGIVALILFDLVPLVKYILTPYRGLVIS
                     STLSFLIVVTGKVEFLRGIVEGILEKDLTFTSRTFIWDNAMSAIREKPFGYGMMMFTD
                     NVHILGNFPGWIWKGATHAHNNFLQVLFQGGYLGFGVYFLIILKGAMLCRKYWDDRNA
                     RLLFYGLFIFTIISITENLEMQMMYLVIILPFLLPNILVKERGKNE"
     gene            3013..4959
                     /locus_tag="ACL8A1_001743"
     CDS             3013..4959
                     /locus_tag="ACL8A1_001743"
                     /inference="COORDINATES: protein motif:HMM:NF017515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HGS1202470.1"
                     /translation="MNRGILVSIIVPIYNQEEYLNISLPTLKQQTYKNLEFILVDDGS
                     TDSSKQIINEYLNADSRFKYVYKENGGLVSATIAGVKEASGDYLAFLDPDDKLGLDYI
                     ENFIKELDCDYDFIAAGFFYDDGKLIKPYYLKENSVLTQSDIKKLRTDFLSDTGGLAI
                     SNRVFISRWNKLYKTNLVKEIVKEFRKFEMVSLGEDTIFTFLLLQKAQLGKTIALSNS
                     YFYNVGNPNSMMKEGAIINYVEKCRIAYQSLTKLLIERNLTNEMSCQLYYFLVLSFFN
                     RLEKEDMESFKKIYDILKRDSLFLKILFSLQKKANKKQKLQLLMIRLCKSGKQYLNIK
                     RVVSKLHAKLKLFCQNFKLVRKQIFRKNLVQLAYFLKFQQQRKIGFDDMKVFLPIIEE
                     RLKEDLLEFQKLEVLPSTIEKNIFVFWWDGFDEIPEIVSICLASLKKNYPECKLHLIY
                     KDNYQDYTNIHPNILNDFNKGKISVQTFSDVLRFNLLKNNGGMWVDSTIYFTKKIDLF
                     QELEKCSFSTLNFSTSKDFMSYKGEVCTWSGFMIASHKQGRLVSIINELFERYYLKYN
                     TFSTYFFIDIVFMLCKVYKLDDNVLDQIILKDGNMFQLASVLDQPFNKSLLPYIEKLP
                     QKLFLHYEDNNLDNFYKYFKEIVS"
     gene            4962..6479
                     /locus_tag="ACL8A1_001744"
     CDS             4962..6479
                     /locus_tag="ACL8A1_001744"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HGS1202471.1"
                     /translation="MSNRLVNTRRNIVWGTLNKLIAIALPFLVRTTLIYQLGIQYVGI
                     SGLFASILQVLSLADLGFANAIVYSMYKPLAHGDNDTVGALLGFYKKIYRVVGVVILI
                     AGLLIVPFINYLVKGSYPADINIQLIYLIYLANTVVSYFLFAYKRSLLLASQRKDIFD
                     NIDSVARIILSGLQILLLFLFPDFYVFALVIPCMTILNNVFNEIVTKKNYNYIVENSN
                     LDKKVKQEIIEKTKGLFVYKVCGITRNSLDNIFISTFLGITTVGIYSNYYYIMISVKG
                     FLDVVSTGMSASVGHSVATESVEKNHRDLENLTFGFSWLATWFMTCLLCLFQPFMLLW
                     VGQANILPFSVVVALCFYFYVLAAGDMRSQYIDASGLWDKEKLRSIAETVGNVVLNYI
                     LVQFLGVLGIVLATALTILFIGIPWSTKIVFDNYFKNGYKKYLWNQAVYAIVTIIVAS
                     ITYLICTLVGGNNIVVLIIRGIICLFVPNILYYLFFLKNEQTRLFFKIIKSKLLK"
     gene            6535..7404
                     /gene="rfbA"
                     /locus_tag="ACL8A1_001745"
     CDS             6535..7404
                     /gene="rfbA"
                     /locus_tag="ACL8A1_001745"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HGS1202472.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIRDILIISTPQDLHRFQELLQDGSEFGIKLSYAEQPSPDGLAQAFIIGEEFISDDSV
                     ALILGDNIYHGSGLSKMLQKAASKESGATVFGYHVKDPERFGVVEFDQDMKAISIEEK
                     PEQPRSNYAVTGLYFYDNDVVEIAKSIKPSPRGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEASQYIETVQRMQNVQVANLEEIAYRMGYISREDVLTLAQPLKKN
                     EYGQYLLRLIGEA"
     gene            7405..7998
                     /locus_tag="ACL8A1_001746"
     CDS             7405..7998
                     /locus_tag="ACL8A1_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HGS1202473.1"
                     /translation="MTDNFFGKTLAARKVEAIPGMLEFDIPVHGDNRGWFKENFQKEK
                     MLPLGFPESFFAEGKLQNNVSFSRKNVLRGLHAEPWDKYISVADGGKVLGSWVDLREG
                     ETFGNTYQTVIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPSLGIEWENIAEAEVSEADKHHPLLKDVKPLKKEDL"
     gene            8011..9060
                     /gene="rfbB"
                     /locus_tag="ACL8A1_001747"
     CDS             8011..9060
                     /gene="rfbB"
                     /locus_tag="ACL8A1_001747"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HGS1202474.1"
                     /translation="MTEYKNIIVTGGAGFIGSNFVHYVYENFPGVHVTVLDKLTYAGN
                     RANIEEILGNRVELVVGDIADAELVDKLAAQADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQAAD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQEWWKAEKE
                     AVEANYAKTQEIITV"
     gene            9126..9977
                     /gene="rfbD"
                     /locus_tag="ACL8A1_001748"
     CDS             9126..9977
                     /gene="rfbD"
                     /locus_tag="ACL8A1_001748"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HGS1202475.1"
                     /translation="MILITGANGQLGTELRYLLDERNEEYVAVDVAKMDITNEEMVEK
                     VFEEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTKNVAKASEKHGATLVYISTD
                     YVFDGKKPVGQEWEVDDRPDPQTEYGRTKRMGEELVEKHVSNFYIIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQYGRPTWTRTLAEFMTYLAKNRKEFGYYHLSNDATEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALQE
                     FYKQEVR"
     gene            10040..11117
                     /gene="glf"
                     /locus_tag="ACL8A1_001749"
                     /pseudo
     CDS             10040..11117
                     /gene="glf"
                     /locus_tag="ACL8A1_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011476416.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            11444..13426
                     /locus_tag="ACL8A1_001750"
     CDS             11444..13426
                     /locus_tag="ACL8A1_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS1202476.1"
                     /translation="MKSSKLFALAGVTLLAATTLAACSGSGSSAKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHIDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNLFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVVIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDETTDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
BASE COUNT         4510 a         2048 c         2766 g         4261 t
ORIGIN      
        1 tttcaaatta aagtgattta ataaaaattt aaaggaataa taatgtcaaa aaaagaaatc
       61 aaaccaattt gtttttactt accgcaattt catcccatcc cagagaatga tgaggctcat
      121 ggagaaggat ttactgaatg gactaatgta aagaaagcct atccacttta tgacgggcat
      181 aatcaaccac gagttccata caataataat tattatagtc tattagataa agaagtcttg
      241 attcaacagg cagaattggc taaaaagtat ggaatttacg gattttgtta ttatcattac
      301 tggtttaaaa atggtaaaaa actattagaa aaaccagtag aaatgatgct agaaaacaag
      361 gatgttgata tacctttttg tttgagttgg gcaaatgaaa actggactag gcgttgggat
      421 ggagggaatc atgaaatcat tgttgaacaa aactatgacg acttaaatga tttggagaaa
      481 catgtggatt atctgtgtca attttttaaa gatgaccgat atattaagat tgacgggaaa
      541 ccgctcttgt tgatttataa gccagagcta attccaaatt tagtaaagac aatggaaatt
      601 atcagaaatc gtgctcgaaa gaatggcttt aaggatttag agattattgt acagtttcca
      661 aagttcttgt taaataatca tcagcttgag ttatttgatg gatttgcaga attccaaccg
      721 atttttgaga ttcatatgga aaagcacgag agaaaaaatc cagtttaccg atctgtgaaa
      781 tcttttctac ttaaagttgg ggctcgaaat ttagtacaaa aacttgagat tagaggtgaa
      841 aaaaaggtgc ataataaagc tctcttagat aggagagatt atgatgcaac atggactaat
      901 attattaatc gcccgattaa ggataataag atgattgctg gtgcttttgt tgattgggat
      961 aataccgcta gaaataagaa tgggcgagtt ttcgatggag ctaatccaga aaaatttgaa
     1021 ggttacatgc gacaactaat tgaaaaaatt caaaaagagt atcaatcaga aattgttttt
     1081 ataaacgcat ggaatgaatg ggcggaagga gcataccttg agcctgataa aaaacatgga
     1141 tatggctatc tagaggcctt gaagacagtt atagacgaga caaggtagga aaagctatga
     1201 atattcgtta taaacttcaa tttcgacgat gtaaaaaaga gtggcgaaaa aagaaccttc
     1261 ataatgaaac aacgcctcga actatgttcc caatagaatt ggtttcagta ggggaaaaaa
     1321 cctatggtgg tttgtatgtt ttgtccttca ataaagagtc aaaattaaaa attggaaatt
     1381 tctgctccat tgcaccagag gttgctttta ttctcagtgc tgatcattat atcaatcagt
     1441 tctctacgta tccgttcaaa tctaaagtat ttgatcaagg tgatgaaggg gtttcgaaag
     1501 gggatattat cattgaggat gatgtatgga ttggttttcg ttctacaatt ctttcgggag
     1561 ttacaattgg tcagggagct attgttggag caggatctgt ggtaactaaa tcagttcctc
     1621 cttacgctat tgtaggtgga gttccagcaa aagtgatttc ttatcgattc gaaacagaga
     1681 ttcgtgaaga aatgaaaaaa attgactttt cggagttcaa attagagaaa tttaaaaagg
     1741 ttacggaaga gttgtatcag ccaatttcgt caataagaca aattaataac ataaatgaaa
     1801 aattgaaata gagtcggagg agttgcatgt ttcgttttca gaatgggaca gtatcaataa
     1861 acatgagagg atttctgatg ttcattgcaa tgctcattta ctgtcaacca gaatatttga
     1921 aatcaattcc agttacggga cgtatttata gagatttcat gcatgtagga attatgtttg
     1981 tgacgttttc gtacatttca aaaaaacggt ttccaaaatt ttttgtatgg ctagttcttc
     2041 tatataactc ttggcttact tttttaacta ttcaaaataa cggagatata aaaagggcga
     2101 tatttcaact cttgctgttt acaggctttg tatctatgat agacatattc tctgaaaata
     2161 tgcaggattt aatagaatca ttgttattca cttttgagat ggttgtgtat ctaaactttg
     2221 tcactgtact atctttccca gatggttttt tccatcgtgt aaatgatgca tacggaataa
     2281 caaaagaatg gtttttggga gctgataata attttctatt taccttgttt cctgcaacac
     2341 ttatagcttt attgtataag aatttaggtg gagtaaaaat cagagcatat tcattgattt
     2401 caatcatttt agctactcaa ttgattaaag gatcgggaac ggcaaaggtt gggattgttg
     2461 cgttgatact atttgattta gtacctcttg tcaaatacat acttacccct tacagaggtt
     2521 tggttatatc gtctacgttg agttttctaa ttgtagttac tggtaaagtg gaatttttaa
     2581 gagggattgt tgaaggtatt ctagaaaaag atttgacgtt cacttcccga acttttattt
     2641 gggataacgc tatgtcagca attcgagaga agccatttgg ttacggaatg atgatgttca
     2701 ctgataacgt ccatatatta gggaattttc ctggttggat ttggaaagga gccacccatg
     2761 ctcataataa ctttcttcaa gttttatttc agggaggata tttaggattt ggtgtttatt
     2821 ttttaataat tctaaaggga gcaatgctat gtcgcaaata ttgggatgat agaaatgcga
     2881 gactactctt ttatggactt tttattttta caataatatc tataacagaa aacttagaga
     2941 tgcagatgat gtatttggtg attatactac cgtttttatt gcctaatata ttggttaaag
     3001 aaaggggaaa aaatgaatag aggtattttg gtatcaataa ttgttcctat ctataatcaa
     3061 gaagaatatt taaatatttc tttacctact ttgaagcagc agacgtataa gaatttagaa
     3121 tttatcttag tagatgatgg ttcgacagat agtagcaaac agataattaa cgagtatctt
     3181 aatgcagaca gtagatttaa atatgtgtat aaagaaaatg gaggcttagt atctgctaca
     3241 attgcagggg tgaaagaggc ttcgggagac tatcttgctt ttttagatcc agatgataag
     3301 ttaggattgg actatataga aaacttcatc aaagaattgg attgtgacta tgatttcata
     3361 gcggcaggtt ttttctatga cgatggtaag ttaattaaac catattactt gaaagagaat
     3421 tcagtcttga ctcaatcaga tataaagaag ttaagaacgg actttttgtc ggatacaggc
     3481 gggttagcta tttcaaatcg tgtttttatc tcaagatgga ataaattata taagacaaat
     3541 cttgtaaaag aaattgtcaa agagtttcgt aaatttgaaa tggttagctt gggtgaagat
     3601 actattttta catttttact tctacaaaag gctcaattag ggaaaaccat tgccctatct
     3661 aattcttatt tttataatgt aggtaatcct aactcaatga tgaaggaagg agctattata
     3721 aactatgttg aaaaatgtag gatagcatat cagagtctta ctaagctact tatagaaaga
     3781 aatttgacta atgaaatgag ttgtcaactt tactatttct tagtgctttc tttcttcaat
     3841 cgcttagaga aagaagatat ggagtctttc aaaaaaatat atgatatttt gaaaagggat
     3901 tcactatttt taaaaattct attttcactt caaaagaaag caaataaaaa acaaaaactt
     3961 cagctattga tgattcgtct atgcaaaagt gggaaacagt acttaaacat taaaagagta
     4021 gtttcaaaac tccatgcaaa acttaagttg ttctgtcaga attttaagtt agtgaggaaa
     4081 cagattttta ggaagaatct agttcaatta gcttattttt taaagttcca acaacaacgg
     4141 aaaattggtt ttgatgatat gaaggtgttt ttaccgataa ttgaagaaag attaaaagaa
     4201 gatttattgg aatttcaaaa attagaagta ttaccttcaa caattgaaaa gaatattttt
     4261 gttttttggt gggatggctt tgatgagata cctgaaatag taagtatttg cttagcatct
     4321 ttaaaaaaga attatccaga gtgtaagtta catctaattt ataaagataa ttatcaagat
     4381 tacactaaca ttcatccaaa tatattgaat gatttcaata aaggaaaaat ttctgttcaa
     4441 actttttcag atgtactccg atttaatcta ctaaaaaata acggaggaat gtgggttgac
     4501 tccactatat attttactaa gaaaatagat ttattccaag aattggaaaa atgttcattt
     4561 tcaacattga atttttcaac tagtaaagat ttcatgtcct ataagggaga agtttgtaca
     4621 tggtctggat ttatgattgc ttctcataaa cagggacgcc tagttagtat catcaatgaa
     4681 ctttttgaac gctactattt aaagtataat acattttcta catacttctt tatagatatt
     4741 gttttcatgc tctgtaaagt ttacaaacta gatgacaatg tgttagatca aatcattttg
     4801 aaggatggaa acatgttcca attagcttca gttttagatc agccgttcaa taaatctcta
     4861 ttgccataca tagaaaaact cccgcaaaaa ctatttttgc attacgagga taataatttg
     4921 gataattttt acaaatactt taaagaaata gtgagttaac catgagtaat agacttgtta
     4981 atactagaag aaatattgtt tggggaacat taaataagct tattgcgatt gctttaccat
     5041 ttttagttcg aacaactttg atttatcaat tagggataca atatgtcggt attagtggac
     5101 tttttgcatc tattttacaa gttcttagtt tagctgattt aggtttcgct aatgcaattg
     5161 tatatagtat gtataaacct cttgcccatg gggataatga cactgtcggt gcacttctag
     5221 gattttataa aaaaatatat cgagtcgtcg gggtggtaat attaattgcg ggccttttaa
     5281 ttgtgccctt tataaattac ttagtaaagg gttcctatcc tgcagatatc aatatacaac
     5341 taatttactt aatttatctg gcaaatacag ttgttagtta ctttcttttt gcttataaac
     5401 gctctttatt gttagctagt caacgaaaag atatatttga caacattgat tcagttgctc
     5461 gcataatttt aagtggatta caaattttat tattgtttct ttttcctgat ttctatgttt
     5521 ttgctttagt aattccctgt atgacaatcc ttaataatgt atttaacgaa atcgtgacta
     5581 agaaaaatta caattatatc gttgaaaata gtaacttaga taaaaaagtt aaacaagaaa
     5641 ttattgaaaa aacaaaggga ttatttgtct acaaagtatg tgggattaca agaaattccc
     5701 tcgataacat ctttatttct acttttttag ggattacgac agtgggaatt tatagtaatt
     5761 attattacat tatgatatca gtaaaaggtt ttttggatgt ggtttctaca ggaatgagtg
     5821 ctagtgttgg acatagtgtt gctactgaaa gtgttgaaaa aaatcatcgt gacttagaaa
     5881 atcttacatt tggtttctct tggttagcta cctggtttat gacatgttta ttatgtctgt
     5941 tccaaccatt tatgcttttg tgggtaggac aagctaatat tttaccattt tcagtcgttg
     6001 ttgctctctg tttttacttt tatgttcttg ctgcaggtga tatgcggagc caatatattg
     6061 acgcctcagg tttatgggat aaagaaaaac tacggtcaat tgcagaaacg gtaggtaatg
     6121 ttgttttgaa ttatattttg gttcaatttt taggtgtttt aggaatagtg ctagctactg
     6181 cactaacaat tttgtttatt ggaattccat ggagtacaaa aattgtgttt gataattatt
     6241 ttaaaaatgg ttataaaaag tatttgtgga atcaagcagt atatgctata gtaactataa
     6301 ttgttgctag tattacttat ctaatctgta cactagttgg tggcaataac atcgtggttt
     6361 taattataag aggaataatt tgtttatttg tccccaatat tttatattac ttattctttt
     6421 taaaaaatga acagacgcgt ctgtttttca aaataatcaa gtcgaaatta ttaaaataat
     6481 tctatgaaaa atatgtgaat taccttatga ttaaataata gaaggagttt cattatgaaa
     6541 ggtattattc tagcaggtgg ttcggggaca cgtttatatc ctttgactcg cgctgcatca
     6601 aaacaactta tgccggttta tgataaaccg atgatttact acccactttc aacattgatg
     6661 ttggctggga ttagggatat tttgattatt tccactccac aggatttaca tcgatttcaa
     6721 gagcttcttc aagacggatc tgagtttggg atcaaacttt cttatgcaga gcaaccaagt
     6781 ccagatggtt tggcacaagc ctttatcatt ggggaagagt ttatttctga tgatagcgtt
     6841 gcgctaatct taggtgataa tatctaccat ggttctgggc tttccaagat gctacaaaag
     6901 gcagcgagta aggagtcggg agcaactgtt tttggctacc atgtcaagga tccagagcgc
     6961 tttggtgtgg ttgagtttga tcaggatatg aaggctattt ctattgaaga aaagccagag
     7021 caacctcgtt caaactatgc agttacaggt ctctatttct atgataatga tgtagtagag
     7081 attgccaaga gtattaaacc aagtcctcgt ggtgaactgg aaattacaga tgtaaacaaa
     7141 gcttacctag atcgtggtga tttgtctgtt gagcttatgg gacgtggctt tgcttggctg
     7201 gatactggaa ctcatgaaag tttactagag gcttcacagt acatcgaaac agtgcaacgg
     7261 atgcaaaatg ttcaggtagc aaacttagaa gaaattgcct atcgcatggg ttatatcagt
     7321 cgtgaagatg tattgacctt agcccaacca cttaagaaaa atgaatacgg acagtatctg
     7381 ctccgtttga ttggagaagc atagatgaca gataattttt tcggtaagac gcttgcggca
     7441 cgcaaggttg aagctattcc aggcatgttg gagtttgata tccccgttca tggagataat
     7501 cgtggctggt ttaaagaaaa tttccaaaag gaaaaaatgc ttccgcttgg atttccagag
     7561 tctttctttg cagaaggaaa attgcaaaac aatgtatcct tctcacgtaa aaatgtcctt
     7621 cgagggctcc acgcagagcc ttgggataag tacatctctg tagcagatgg agggaaagtt
     7681 ctgggttctt gggttgatct acgcgagggt gaaacctttg ggaataccta tcagacagta
     7741 attgatgcaa gcaagggaat ctttgttcct cgaggcgtag ctaatggctt ccaagttcta
     7801 tcagatacag tgtcatatag ctatctggtc aatgattact gggctctcga gctcaaaccc
     7861 aagtatgcct ttgtgaacta cgctgatcca agccttggta ttgaatggga aaatattgca
     7921 gaagcagagg tttcagaagc agataaacat catcccctac ttaaggatgt aaaacctttg
     7981 aaaaaagaag atttgtaaaa aggaaagaat atgactgaat acaaaaatat tatcgtgaca
     8041 ggtggagctg gctttatcgg ttctaacttt gtccattatg tttacgagaa ctttccaggt
     8101 gttcacgtga cagtcctaga taagttgact tatgctggaa atcgcgcgaa tattgaggaa
     8161 attttaggta atcgtgttga gttagttgtt ggtgacattg ctgatgcgga gttggtagac
     8221 aagttggctg ctcaagcaga tgctatcgtt cattatgcag cggaaagcca caatgataat
     8281 tcgctcaatg atccatcgcc atttattcat actaacttca ttggaaccta tactctttta
     8341 gaagctgctc gtaagtatga tattcgcttc caccatgtat cgacagatga agtttatggg
     8401 gatctccctt tacgcgaaga tttgccaggt catggagaag ggccgggtga gaaatttacg
     8461 gctgaaacca agtacaatcc aagctcgcct tactcatcaa ccaaggcagc ctcagatttg
     8521 attgtcaaag cctgggtgcg ttcttttgga gtcaaggcaa cgatttccaa ctgttcaaat
     8581 aactacggtc cttatcaaca tatcgaaaaa ttcatcccac gtcagattac taacatccta
     8641 agtggtatta agccaaaact ttacggtgaa ggtaaaaacg ttcgtgactg gattcatacc
     8701 aatgaccatt cttcaggagt ttggacaatc ttgacaaaag ggcaaatcgg tgaaacctac
     8761 ttgattgggg ctgatggtga gaagaacaat aaggaagttt tggaacttat ccttaaggaa
     8821 atgggacaag ctgcggatgc ctatgatcat gtgactgacc gtgcaggaca tgaccttcgc
     8881 tatgcgattg atgctagcaa gctccgtgat gagttggggt ggaaacctga atttaccaac
     8941 tttgaagctg ggctcaaggc aacaatcaag tggtatacag ataaccaaga atggtggaaa
     9001 gcagaaaaag aagctgttga agccaattat gctaagactc aggagattat tacagtataa
     9061 aaagcaggaa atagctgctt tttattgcta tattgggaag agttacatat tagaaaggtc
     9121 tagagatgat tttaattaca ggggcaaatg gccaattagg aacggaactt cgctatttat
     9181 tggatgaacg taatgaagaa tacgtggcag tagatgtggc taagatggac attaccaatg
     9241 aagaaatggt tgagaaagtt tttgaagagg tgaaaccgac tttagtctac cattgtgcag
     9301 cctacaccgc tgttgatgca gcagaggatg aaggaaaaga gttggacttc gccatcaatg
     9361 tgacggggac aaaaaatgtc gcaaaagcat ctgaaaagca tggtgcaact ctagtttata
     9421 tttctacgga ctatgtcttt gatggtaaga aaccagttgg acaagagtgg gaagttgatg
     9481 accgaccaga tccacagaca gaatatggac gcactaagcg tatgggggaa gagttagttg
     9541 agaagcatgt gtctaatttc tatattatcc gtactgcctg ggtatttgga aattatggca
     9601 aaaacttcgt ttttaccatg caaaatcttg cgaaaactca taagacttta acagttgtaa
     9661 atgatcagta cggtcgtccg acttggactc gtaccttggc tgagttcatg acctacctag
     9721 ctaaaaatcg taaggaattt ggttattatc atttgtcaaa tgatgcgaca gaagatacaa
     9781 catggtatga ttttgcagtt gaaattttga aagatacaga tgtcgaagtc aagccagtag
     9841 attccagtca atttccagcc aaagctaaac gtccgctaaa ctcaacgatg agcctggcca
     9901 aagccaaagc tactggattt gttattccaa cttggcaaga tgcattgcaa gaattttaca
     9961 aacaagaagt gagataagta gtagaatgat tttctagtct aataaaagag gcagagaatg
    10021 aactccaaag gagcttaaga tgtacgatta tcttgttgtt ggtgcagtct ttgcccatga
    10081 agcagtctta aaaggaaaaa aagtaaaagt tattgaaaaa tgaaatcata tcgcgggtaa
    10141 tatctatact cgtgaagagg aaggaattca agttcatcaa tatggtgctc atatcttcca
    10201 tacttctgat aaggagatct gggattatgt aaatcagttt gcagacttta accgttatac
    10261 caattctcct gttgcaaact ataagggtga gatttataac cttcctttta atatgaatac
    10321 cttcaataaa ctctggggaa ttgtgacgcc agcagaagca caagctaaga ttgatgaaca
    10381 acgtgctatt ttaaatggta aaactcctga aaatttggaa gaacaggcga tttctcttgt
    10441 aggtacagac atctacgaaa aattaatcaa agactataca gagaaacagt ggggcaaacc
    10501 aactactgaa cttccagcct ttattattgt ttgccagtac gcctgaccta tgataacaac
    10561 tattttaacg atacctatca agggattcca attggtggat acactcaaat agttgaaaaa
    10621 tgttggatca tgaaaatatt gatgtagaaa caaatgttga tttctttgtg aataaagagc
    10681 aatatctgaa agattttcct aagattgtct ttactggtat gattgatgaa ttctttgact
    10741 ataagttggg cgaactagag taccgtagtc ttcgttttga aaatgagacc ttggatatgg
    10801 aaaattacca agaaaatgca gttgtgaact atacggatgc agaaatccca tatactcgca
    10861 ttattgaaca caaacatttt gagtttggga gtcaagcaaa gactatcatt actaaagaac
    10921 attctaaaac atgggaaaaa ggtgatgagc cttattatcc agttaataat gatcgtaata
    10981 atcatttgta taaatcgtat aaaaaacttg ctgatgagca agggaatgtt atctttggtg
    11041 gccgcttagg acactatcgt tattacgata tgcaccaagt aattggagca gctttgcagt
    11101 gcgtgagaaa tgagttagat tcatactcaa tgaaaattaa agagcaaact aggaagctag
    11161 ccgcaggttg ctcaaaacac tgttttgagg ttgcagatgg aagctgacgc ggtttgaaga
    11221 gattttcgaa gagtataaac aagtaaaact gactactagt tattatttag aaatagtatt
    11281 aaaaattcct tgactatatg atatagttga gggattttta aatgatattc atattttttg
    11341 caaagatgtt gtttgaaaaa taattttcaa aaattctgaa aattctgttg acaactttct
    11401 gaaaagagtt tataatggag agaaagtttt aaaggagaaa atgatgaaaa gttcaaaact
    11461 atttgccctt gctggcgtga cattattggc ggcgactact ttagctgcat gctctggatc
    11521 aggttcaagc gctaaaggtg agaagacatt ctcatacatt tatgagacag accctgataa
    11581 cctcaactat ttgacaactg ctaaggctgc gacagcaaat attaccagta acgtggttga
    11641 tggtttgcta gaaaatgatc gctacgggaa ctttgtgccg tctatggctg aggattggtc
    11701 tgtatccaag gatggattga cttacactta tactatccgt aaggatgcaa aatggtatac
    11761 ttctgaaggg gaagaatacg cggcagtcaa agctcaagac tttgtaacag gactaaaata
    11821 tgctgctgat aaaaaatcag atgctcttta ccttgttcaa gaatcaatca aagggttgga
    11881 tgcctatgta aaaggggaaa tcaaagattt ctcacaagta ggaattaagg ctctggatga
    11941 acagacagtt cagtacactt tgaacaaacc agaaagcttc tggaattcta agacaaccat
    12001 gggtgtgctt gcgccagtta atgaagagtt tttgaattca aaaggagatg attttgccaa
    12061 agctacggat ccaagtagtc tcttgtataa cggtccttat ttgttgaaat ccattgtgac
    12121 caaatcttct gttgaatttg cgaaaaatcc gaactactgg gataaggaca atgtgcatat
    12181 tgacaaagtt aaattgtcat tctgggatgg tcaagatacc agcaaacctg cagaaaactt
    12241 taaagatggt agccttacag cagctcgtct ctatccaaca agtgcaagtt tcgcagagct
    12301 tgagaagagt atgaaggaca atattgtcta tactcaacaa gactctatta cgtatctagt
    12361 tggtacaaat attgaccgtc agtcctataa atacacatct aagaccagcg acgaacaaaa
    12421 ggcatcgact aaaaaggctc tcttaaacaa ggatttccgt caggctattg catttggatt
    12481 tgaccgtaca gcctatgcct ctcagttgaa tggacaaact ggagcaagta aaatcttgcg
    12541 taatctcttt gtaccaccaa catttgttca agcagatggt aaaaactttg gcgatatggt
    12601 caaagagaaa ttggttactt atggggatga atggaaggat gttaatcttg cagattctca
    12661 ggatggtctt tacaatccag aaaaagccaa ggctgaattt gctaaagcta aatcagcctt
    12721 acaagcagaa ggtgtgacat tcccaattca tttggatatg ccagttgacc aaacagcaac
    12781 tacaaaagtt cagcgcgtcc aatctatgaa acaatccttg gaagcaactt taggagcgga
    12841 taatgtagtc attgatattc aacaactaca aaaagacgaa gtaaacaata ttacatattt
    12901 tgctgaaaat gctgctggcg aagactggga tttatcagat aatgtcggtt ggggtccaga
    12961 ctttgccgat ccatcaacct accttgatat catcaaacca tctgtaggag aaagtactaa
    13021 aacatattta gggtttgact caggggaaga taatgtagct gctaaaaaag taggtctata
    13081 tgactacgaa aaattggtta ctgaggctgg tgatgagact acagatgttg ctaaacgcta
    13141 tgataaatac gctgcagccc aagcttggtt gacagatagt gctttgatta ttccaactac
    13201 atctcgtaca gggcgtccaa tcttgtctaa gatggtacca tttacaatac catttgcatt
    13261 gtcaggaaat aaaggtacaa gtgaaccagt cttgtataaa tacttggaac ttcaagacaa
    13321 ggcagtcact gtagatgaat accaaaaagc tcaggaaaaa tggatgaaag aaaaagaaga
    13381 gtctaataaa aaggctcaag aagatctcgc aaaacatgtg aaataactgt tgcaaaatat
    13441 aagaaaggat ttagtatttc ccttgaatgc tgaatccttt tttacatttg taaagaaaga
    13501 ttctaaatgt acggaccccc aaaagttgga gcctcttttt gtcagaatag agaaaatttt
    13561 tgttaatttt acttgtttcc tattg
//
LOCUS       DBERMR010000053        13388 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.053, whole genome shotgun sequence.
ACCESSION   DBERMR010000053 DBERMR010000000
VERSION     DBERMR010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13388
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.053"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            94..1272
                     /locus_tag="ACL8A1_001751"
     CDS             94..1272
                     /locus_tag="ACL8A1_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HGS1202477.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQSKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDDYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
     gene            complement(1500..1946)
                     /locus_tag="ACL8A1_001752"
     CDS             complement(1500..1946)
                     /locus_tag="ACL8A1_001752"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1202478.1"
                     /translation="MTIRFEEKVSIENAQLVCQWSNSLGKVFQEQWMGPRIPFPLTLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQGQGLDSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVRKYSELKLE"
     gene            2016..2675
                     /locus_tag="ACL8A1_001753"
     CDS             2016..2675
                     /locus_tag="ACL8A1_001753"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HGS1202479.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTPLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELGLGLLFLLYSWELLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTFHLILIASSGWNLVMVSFLILGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERFPFVPFLLLATCLIIFGKLLL
                     V"
     gene            complement(2727..3878)
                     /locus_tag="ACL8A1_001754"
     CDS             complement(2727..3878)
                     /locus_tag="ACL8A1_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS1202480.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNIIIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(3893..4624)
                     /locus_tag="ACL8A1_001755"
     CDS             complement(3893..4624)
                     /locus_tag="ACL8A1_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202481.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WTRPNHIDTAFASADLFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFP
                     LVQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNS
                     NIEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDI
                     PNFSELSETEDFEIIPRIEKQEGQK"
     gene            4853..4954
                     /locus_tag="ACL8A1_001756"
                     /pseudo
     CDS             4853..4954
                     /locus_tag="ACL8A1_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            complement(4926..5702)
                     /gene="trpA"
                     /locus_tag="ACL8A1_001757"
     CDS             complement(4926..5702)
                     /gene="trpA"
                     /locus_tag="ACL8A1_001757"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HGS1202482.1"
                     /translation="MPKTLTEKLNAIKATGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFIYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIRQAVAYQK"
     gene            complement(5695..6918)
                     /gene="trpB"
                     /locus_tag="ACL8A1_001758"
     CDS             complement(5695..6918)
                     /gene="trpB"
                     /locus_tag="ACL8A1_001758"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HGS1202483.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGLGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            complement(6896..7495)
                     /locus_tag="ACL8A1_001759"
     CDS             complement(6896..7495)
                     /locus_tag="ACL8A1_001759"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HGS1202484.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVDLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQSFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            complement(7482..8249)
                     /gene="trpC"
                     /locus_tag="ACL8A1_001760"
     CDS             complement(7482..8249)
                     /gene="trpC"
                     /locus_tag="ACL8A1_001760"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HGS1202485.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDC
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            complement(8246..9250)
                     /gene="trpD"
                     /locus_tag="ACL8A1_001761"
     CDS             complement(8246..9250)
                     /gene="trpD"
                     /locus_tag="ACL8A1_001761"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HGS1202486.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            complement(9261..9827)
                     /locus_tag="ACL8A1_001762"
     CDS             complement(9261..9827)
                     /locus_tag="ACL8A1_001762"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HGS1202487.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            complement(9824..11185)
                     /gene="trpE"
                     /locus_tag="ACL8A1_001763"
     CDS             complement(9824..11185)
                     /gene="trpE"
                     /locus_tag="ACL8A1_001763"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HGS1202488.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYIVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMEVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            complement(11557..11763)
                     /locus_tag="ACL8A1_001764"
     CDS             complement(11557..11763)
                     /locus_tag="ACL8A1_001764"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202489.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVADRTDEVSSKHCFEVADRTDEVS
                     NHTYDKATLTRFEEIFEEYKGVPR"
     gene            complement(12370..13371)
                     /locus_tag="ACL8A1_001765"
     CDS             complement(12370..13371)
                     /locus_tag="ACL8A1_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGS1202490.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTSQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
BASE COUNT         3864 a         3002 c         2309 g         4213 t
ORIGIN      
        1 gtaatgaaaa gcttaaaaag attaacatct acatttatcc atagcaaaaa atcataggat
       61 tttaagaatc tgacaactag gaagaaaact aatatgacct tatctaagaa acaattacaa
      121 ttaagagcta aaatactgga aacagtttac acattaggtc ccatttcacg aattgaaatc
      181 gctactaaaa caggaataac acctgcaaca actagcagta tcacaaatga tctgattaag
      241 gaaaatattc tcttagaact tggtgaagac gaacacgata caagtgtagg aagaaaaaaa
      301 atcctgctag atattcaatc taaacgattt tactacattg gttgtgaact atctgaaaaa
      361 catttcactt ttgctttagg agataatcta ggaaatatct taaaggaaga gaaagaaatt
      421 gttactaaac agttaatcca agaaaaggga aatcaactca ttaaccagac actaaagcaa
      481 ttcttaaata actgctctga ttatgaaata gaagcaattg gaatcgcgct acctggtcgc
      541 tatttagatg attataaaat aacaacaaac aatcctttat ggcaacatat cgacttagaa
      601 atgattcaat cccactttga taagcctttg tttttctcta acaatgttaa ctgtatggct
      661 ataggtaaac gcctatttag tcgccaacaa aatgacccta attttgctta ttttcatttt
      721 gctagaggta tgcactgttc ttatatctac gacggaaaca tttatgggaa aggaaatcta
      781 atgattggag aaatagggca tacagttgtt tcatctgagg gagaagaatg tagttgcggg
      841 cgcaagggat gcttacaaac ttttgctgga gaatcttggt taatcaaaaa atcaaaaatt
      901 ctatatcatc aatctcccta ttctctactt cctagtttag tcaaaaatgc cgatgatatt
      961 gatatccaag ttattttaac cgcttatcaa ttaggtgaca ctggaataat cacacttatc
     1021 catcaagcct tactctatct tagtcaaacc attttaaata tcagtatgat gattgactct
     1081 caaaaaatct acctacatag tcctttacta acaaatcaac acatcatcca aaaactatat
     1141 agtgaaatga actacaaacc caaactgctc tataatcgcc tacctgaagt cattattgaa
     1201 ccttacaatg attttacagc cgcacactca gctattgcac tttgtttgta tcatacaatt
     1261 ctacattctt aattttctat tacatttaat aaataggcta aaaagaggga gtatatcata
     1321 aaaagcgaac aaacctgttt tctgaaaatc agaattctgt tttgttcgct ttctttatat
     1381 ttggaattat actcctatct cccctttttc ataatgtata gtaaaatgaa ataataacag
     1441 gacgaatcga tcgggacagt caaatcgatt tctaacaatg ttttagaagt agaggtgtac
     1501 tattctagtt tcaattcact atattttcgt acaggtgctt caaccatttg aacgatttca
     1561 aatccttctt tttggtaaag attctgagct ctttgatttg cctcgaagac atttagagaa
     1621 atactatcta tatcttcatt ttcaaaggcc aaactaacaa atttccttaa agcctggcta
     1681 tctaagcctt gcccctgttt ctgggggttg ataaaaaatc tcccgatatg aagattgctg
     1741 tcttctagcc tgattttctg gataagtccc acaaactctt gttcatcaaa gattgaaaag
     1801 actccttcca aggcttgaag ggtcagtgga aaaggaatcc ttggtcccat ccattgttct
     1861 tgaaagactt tgcctaagga gttggaccat tggcatacga gctgagcgtt ttctatactc
     1921 accttttctt caaaacgaat tgtcatcttt tcctcaccat cttatctatg tttctccatt
     1981 atactatttc tcccattttt tacgaataga taagtatgat tgatttttat ttttttctcg
     2041 tcgggagcat tctagcttcc tttcttggtt tggtcattga ccgttttcca gagcaatcca
     2101 ttatcagttc agccagtcac tgcgattcct gtcagactcc cttgcgtccc ttagatttga
     2161 ttccgattct ctcacaggtc ttcaatcgct ttcgctgtcg ctactgcaaa gttcgctatc
     2221 ctgtctggta tgccctcttt gaactaggct taggactcct ctttctgctt tactcttggg
     2281 aattgctttc cttggggcaa gtcgtcctaa tcaccgctgg tttgaccttg ggtatatacg
     2341 actttcacca tcaggaatat cccttactgg tctggatgac tttccaccta atcctaatag
     2401 cttcctctgg ctggaatctg gtcatggtct ccttcctcat acttggaatt ttggctcatt
     2461 ttattgatat ccgcatgggt gcaggggatt tcctcttttt agcttcttgt gctctcgtct
     2521 ttagcgtaac ggagttactg atcttgattc agttcgcttc tgcgacgggt atcctggcct
     2581 ttctcctgca aaagaaaaag gaaagatttc ctttcgtgcc tttcctctta cttgctactt
     2641 gtttgattat ttttggtaag ctactgcttg tctgataaaa tccaatttct gccatatgtc
     2701 cttcatgaaa ttatttcaca gttaaattat aaattatttc ttttgtacaa agggatgatg
     2761 ttatcaaatc gatctgttct tctatcttct tgatactgat caaaaaattt catttcgact
     2821 gaaaatattt cgcttataaa ctgtaaacga atactttgtt tagacattat agtcgctaga
     2881 ctgactagat gattactcaa aacgacgtcc agaatactct ttactttgct tggtttttta
     2941 acaaaaattt gatcatccaa aggttcaatc attttgtaac ctttttgcgc aatttgacga
     3001 taaaagtaag aatgttgctt tggagtcaat aatcctaact taaaagctcg atactctaaa
     3061 gcctgtatcg aaacattcaa atccgacttc aataaaatat aactatcagg attgctgaca
     3121 cgcttgccaa ccctctcttc aaatttgact aaaaactctt cttttggcaa taaaaaacat
     3181 gatgcaaaat aatttgcttc ttgctccaaa cgatcgccat cttcattcat atctttatat
     3241 ttatgtaaaa gaatatgtcc tagctcatga gctaagtcaa aatttcgacg tacagatgat
     3301 ttattcgttc ccaacacaat ataaggtctt cccaattttg accatgcgct ataagcatca
     3361 gcttggccat taattaatcg ttccacgata tagatgcctg aacgttctaa tttataaagc
     3421 aaatcatgat tatcttttga aatacctaat ttttccctgg cataaagagc caattcctca
     3481 atggattctc ccttatgata agattcactc actacattac ttaggtcatg aattataata
     3541 ttaggtataa ttacaaaact ttcaaaataa tcaatcaaac tatctacctt atgtaaatac
     3601 atagtttgaa tatctattgt tttccgtgtt gctaggtctg catttctaaa ggcaattaca
     3661 gaagaatcaa atcgaatgct ctcttcttcc tgttcaaaat aagttaaatc aacatgaaat
     3721 tggttggcca aatgcatttt ggttgataat ttaggtttcg tttcgttgga ctcaaactgc
     3781 caaatggctt gttccgttaa attaattctc tgagctaatt ctgctctact taaaccattt
     3841 aacagccgta attctttcaa tacccgacca ttaaacattt acatactcct tactactttt
     3901 gaccttcttg tttttctatt cttggaataa tttcaaaatc ttctgtttcc gataattctg
     3961 aaaaattagg aatatcttga tatttagctt cttcgaaatg gtacgaactt gattgaatat
     4021 actcagacaa atcttgaatc aagtgtaact gaccagttcg tgcatcaggc ataacaacat
     4081 ctacagatat aatattgttt tctgagtccg cctcataagt taaaatcata aatttttcga
     4141 tattcgaatt tttagtagct tgttcaattt cttgaatcat ttcatcagaa actaactcca
     4201 tctgaattgg aaaggaatga ctatcttcat catttttgta ggaagaatgt tgattaagat
     4261 aaagtgtatt catctgagca tattcaaata agtagccact cttatttttt tgtaccaaag
     4321 gaaattggtt cgtaagtcgc ttcttaccct ttataattaa caatactttc ccatattttt
     4381 ctgtatttgt ttcaaattct aaatatcccc aagtctgtcc tgctaattgt aatttatact
     4441 caaacaaatc tgctgatgca aatgcagtat caatatgatt aggtcgcgtc catgcataac
     4501 cattcgacac tatcattgtc tctctttttt ctagacgttc atctacataa tctttttgcc
     4561 ctttcatcaa agtatctaca attttttgtg cctcaagcga atcaaagaga tcctgattca
     4621 acataattct tcctcctcca aatacttttt aatgaattat atcattttct taaagaaatt
     4681 actacaataa ttagcttttt cttaaagttc tgtgtcagag taatttagaa aattatatct
     4741 tctatagtaa aatcaattaa aaactgaaca aatttattgg gaaattcaaa tcactttctt
     4801 aaaatatttt aggaaccgta gtgtaatatt ccagattcaa ttcactataa aactagcctt
     4861 tctcctgcaa aagaaaaagg aaagacttcc tttcgtgcct ttcctcttac ttgctacttg
     4921 tttgattatt tttggtaagc tactgcttgt ctgataaaat cctgaatcgg ctctccttgg
     4981 tggagagctt ttactatttt tgaaccgacg ataacaccat ctgacaccgc attgaagcgt
     5041 tccagatcgg cttgactaga tacaccaaaa cctgtcaaga ctgggatgtc ggccacttga
     5101 tgaagttgcg ccaagtgctt gtccaaatct gcacggtaat tgcctgattt ccctgtcact
     5161 ccattgatgg caacggcata gatgaatccc tccgcccctt caatcaactc tttctggcgc
     5221 tcaattcctg tggtcaagct tactaaagga atcaaggcga tatctgtatc tgccaaaaat
     5281 ggttctacaa agttggcatg ttcatgaggc aagtctggga taatcaagcc cttcacagct
     5341 gtatcagcca gatctttgac aaagttctcc acaccgtact gaaagagggg attgaagtag
     5401 gtcatgatga ccagtggaat ctctgtttca atggttttca aggtttcaac taaagcctgg
     5461 gtagaggtcc cgtgggctaa actgcgcaag ccagcttctt cgataacagg tccatctgca
     5521 acagggtctg aaaagggaat acccacttca attgcagaga cacccaaatc ttctaaaaag
     5581 tgaattgttt cagcaagacc gtccaaacct ttctcgtggt caccagccat gatataggga
     5641 acaaaaattc cttttccagt tgcttttata gcattcaatt tttctgttag tgtcttaggc
     5701 atgagcttct cccttctttg ctgcatctgc ttccaagcgg tctttgactt gaaccacatc
     5761 cttgtcccca cgacctgata ggcagacaat catagacttt tctggtccaa gttctttggc
     5821 caatttcacc gcaaaggcga tagcatggct agattccaag gctgggataa tcccttccac
     5881 acgagacaag agttgaaatc cttccaaagc ttcttcgtct gtcacaggga catagctggc
     5941 acgtttgata tcgtggtagt gagaatgctc tggaccgata ccaggatagt ccaaacctgc
     6001 tgagatagag aaggcttcaa gaatttgacc atgggcatct tggagcacat ccatgaggga
     6061 accgtgaagg acacctggac gacccttggt caaggtagct gcgtggtgct ccgtatccac
     6121 accaagtcca gccgcttcag ctccatacat ggctactgac tcatcttcta caaagggatg
     6181 gaagagaccg atggcattcg acccaccacc aacacaggct actagggcat ctggcagatt
     6241 ttgtcctgtc aagtcacggt actgttgttt agcctctcga ccaatgacac tttggaagtc
     6301 acgaacaatt tctggaaatg gatgaggccc caaggcagaa ccaaggatat agtgggtatc
     6361 atcgatatta gccacccatg aacgaagggc tgcattgacc gcatccttga gcacgcgcga
     6421 accatctgtc acggcctcga ccttagctcc caaaagctcc atacggaaga cattgagggc
     6481 ttggcgtttg acatcttcct cacccatgta gatggtacat tccatgttaa agagggccgc
     6541 agcagttgca gttgccacac cgtgctgacc agcacccgtt tctgcgataa ttttcttttt
     6601 acccatgcgt ttggcaagcc aaacttgtcc taaggcattg ttaatcttgt gggctcctgt
     6661 atggttaagg tcttcccgtt tgagataaat cttggctccg ccgatatgct gggtcaagtt
     6721 ttttgcgtag taaaggggag tttcacgtcc cacgtactgg cgcaaaagtt ggtttaattc
     6781 ctcttggaaa cttgggtctg cctgactttc acggtaggcc ttctccaact ccaaaactgc
     6841 tgtcatcaat gtttctggga caaaacgtcc gccgaatttt ccgtaaaatc catctttatt
     6901 tggttcctga tatgccatgc tttaccctct ctataaatct tctaatcttt tcatgatctt
     6961 tttgtccatc tgtctccact ccgctcgata catctactgc atagggagta aaatgttgaa
     7021 ttgcttttac tacattatct tcattaaggc cacctgcgat aaagaagggc tgtgctagtc
     7081 cagtcgtatc cagttgaccc caatcaaagg actggccact tcctgccaca ggggcatcaa
     7141 agagtagata atctgcctga gaattgggga catgcccatt tccatctacc tgcacagcct
     7201 gaatactggc acaaggcaaa ttctcaaata aatcatctgc cacctgaccg tgaacttgaa
     7261 ccaagtccaa gtcaactttg tcaatcgctt ccagcagttc tacccgactt ggtgaaacaa
     7321 atactccaac ctttttcaca tctgcaggaa taagctttgc caactcagct gcctcttcta
     7381 aagtcacctg tcttttacta ggtgcaaaga caaaaccgat atagtcggct cctgctgaaa
     7441 cggctgtttc caccgcttct ttggtcgata gtccacaaat tttaaccttt gtcaatctgc
     7501 aactccttga ttctctgggc cacattttct gcctgcataa gagctgtccc taccaaaatt
     7561 ccgttaaagt atggggctag tcgttccgca tcctgccctg tgaaaatggc agattcagaa
     7621 atgtaatagc gaccttcctc aaagtaaggg gctaaatcta cactggtctg caagtcgacc
     7681 tcaaaggtag tcaagttgcg gttgttgacc ccgataatct cagcaccaag tctgtgggct
     7741 acctctagtt cagctagatt gtgagtctcc actaagactt ccagaccaag ctctgtcgcg
     7801 tagtcataca gttccttgag gcgttcttcg gacaaggctg ccacaataag caagataact
     7861 gtcgcacctg cattgcgagc gcggatgatt tgcttttcat ctatgataaa gtctttgttg
     7921 agcgtcggaa tctctacctg actggaaatt tcccgtagat aatccaaatg ccctttaaag
     7981 aaaacctcat ctgtcaacac cgaaatcatc actgctccgt tttcttcata agtctgggcc
     8041 tgttgcacaa tatccacatc gagattgata tctcccaaac tagggctagc tttcttgacc
     8101 tcagcgatta cctgcaagca gtcctgatga ttcttcaaaa attctgccaa gcgataggtc
     8161 tggcgcagag gctggatttg ctccagcttc atctgctcca cctcacgcgc cttctgctct
     8221 aagattcgtg ctaaaaattc ctgactcatt tttggtactc ctgtaacagt ctgagttttt
     8281 caagggcctt gcctctagca atcacttgac gggccaaggc aactccttcc ttgatgctat
     8341 caatcttacc attagcatag aaaccaagac cagcattcaa gactgtcgtt tccaagaatg
     8401 gacttgcttc gtttttcaga acgctaagca aaatttctgc attttcctga gcattcccac
     8461 cacgaatatc ttccatagca tagccttcca ttcccaaatc ctctggagta aagcttgaca
     8521 agctgatttc gccattttca agaagtgcaa tcttggttgt tccgttcaag ccagcttcat
     8581 ccaacccttc tggtccagca accacgatgg cacgtttgcg acccatattt ttcaaaacct
     8641 gagctgtact ttctaggagt tctggacgac taattccaag aagctgtgtt tctaaagcca
     8701 ttggatgaat cagtggacca gtcaagttca taatcgttgg aattcccaat tccaaacgag
     8761 ctggcatgat gtatttcata gctgggtgca tatttttagc gaagagaaag acgattccag
     8821 ttttatcaaa gaccttacct agttcagctg gtttgaggtc aagattgatt cccaaggctt
     8881 cgaggacatc tgcggaacca gatttagaag aaatcgagcg attaccatgc ttggccatgt
     8941 gaataccgcc accagccaag acaaaggctg cagttgtgga aatattaaaa ctgaaagact
     9001 tgtccccacc tgtaccacag ttgtccatgg catcatgaat ctcagttgga atatgctggg
     9061 catgtcctct catgacttgg gcaatggctg tgcgttcttc aggtgtttcc cccttcatct
     9121 taagagctaa gaggagagaa gcaatctgcg cttcagttac acgcccagtt acgatacgct
     9181 caatgacatc cgtcatttcc acacctgata aattttcaaa ttttgctagt ttttcaataa
     9241 tctctttcat cctagtttcc tcactttaca accttctcga taaaattccg aatagaagac
     9301 aagccgtctg gcgttccaat gctctctgga tggtactgga agccataaat cggtaggttt
     9361 ttatgttgaa tccccatgat ggcttggtca tcagtcgaac gagctgtcac ttcaaagtct
     9421 tctggcattt cctcaatcaa aatactgtga taacgcatga ccgcacggcc atcctcaata
     9481 ccttgataca aaacagatgg cgcttcaaag ttaatattgc tctgtttccc atgcatgact
     9541 tttggagcca aacctagctt accaccaaag acttctgcaa tggcttggtg acccaaacaa
     9601 atccctagaa tcggcttctt gcctgcaaaa tcacgaatca tgtcttccat ctttccagca
     9661 tcaactggcc aaccaggacc aggagaaaag accagaccat ctgctttttc agcttcttca
     9721 tacagcttgg aatcatcatt tctcagaacc tgaacttctg caaaattccc aatgtattgg
     9781 gccaagttat aggtaaaaga atcatagttg tcaatcaata aaatcatggt cttagttctc
     9841 caattctagt catagatttt gctttgttaa tggtttcttg gtattcgttt tgggcgatag
     9901 agtcgtagac aatccctgcc ccagcctgca cataggctct ttgatttttg agaatcatgg
     9961 ttcggatggc gatggccaaa tccatatcac ccgtcgcaga caagtagccg attgccccag
    10021 cgtatactcc ccgtttttcc gtttccagtt catagatacg tctcatcgct cgaatctttg
    10081 gcgctccaga aacggttcca gcaggaagcg ttgctttcaa ggcatccatg gcagtgagtt
    10141 ctgcaagcaa acgtcccttg accacactgg tcaaatgcat gacgtagcgg aagagctcca
    10201 cctccatata cttagtaact tggacactgg ccgtttcaga gatgcggcca atatcgttac
    10261 gccccaagtc taccaacatt cgatgttctg ctgtttcctt ctcatcagag aggaggtcag
    10321 tcgccaaggc cttgtcttct tcatccgtag cccctcttgg tcgcgtccct gcaatcggat
    10381 tggttgtcac gatgccattt ttgacagaaa ccaaactttc tggactagct ccgatgattt
    10441 gataatcccc aaaatcatag aaataaaggt aattagaagg attagttacg cggagatttc
    10501 tgtagaagtc aaatggattt ccagtaactt ctgctgagaa acgctggctg agtacacatt
    10561 ggaacatatc gccgttacga atcaagtcac gagctgtttc taccattccc tcaaacttat
    10621 gtggagcgat atgcggtttg aagtcaagtg gtgataaatc caagtcttca aattcatttg
    10681 gagcaggaat gcgtaattcc tcaagcactt ggttcaagga tttttccaag gcctcttgac
    10741 tgcgctcact ataaagtgca tcctctatga catgtatctt ctccttcttg tggtcaaaga
    10801 ctatatagct ctcatagaca aagaaatgca tgtctggcgt cccaattgta tcctcaggga
    10861 tttgaccaat ttcttcataa agcgaaatca tatcgtaacc cacaaaacca atggctccac
    10921 caccaaaagg tagctctgaa tggtgctgac tcttatgaat cacttcataa aggaaatcca
    10981 agggatcccg atcaatcact tgaccatttt gataaagaac tccattttca aacttaatct
    11041 caaaaacagg attataggct aggatagaaa aacgagctgt ttccttgtct ctcggaatac
    11101 tctctaagat aaccttgtgt tgccccttta agcgcatata agccaagatt ggtgataaga
    11161 catctccatg aatgattcgt tccattgtaa tttccctttc agttctaatt ctagttcgta
    11221 gcgactgtat gaaaaatccc cacgcaaaat aacttgcgtg aggacgaaat tcgcggtgcc
    11281 acctcaatta taggatttct cctatctctc attcctgtct cagatatctc ctgtaacagg
    11341 ctgtgcgata aagggcactc ccttgagaat gatgttttct tctctcgttt cagatgaacc
    11401 caactttaca gctttctctg cttgttttca gcaaccacaa gctctctgtg agagaaagaa
    11461 ctgtaatttt tccatctatt attttttagc ttctagtagt ctgcaatcgc agctaggtcc
    11521 ttgcctccac gaccagagac attgatgaag agatgttcat ctcggtacac ctttatactc
    11581 ttcgaaaatc tcttcaaacc gcgtcaacgt cgccttatcg taggtatggt tactgacttc
    11641 gtcagttcta tctgcaacct caaaacagtg ttttgagctg acttcgtcag ttctatctgc
    11701 aacctcaaaa cagtgttttg agcagcctgc ggctagtttc ctagtttgct ctttgatttt
    11761 cattgagtat tactagcttt tttcgtatta gtccagcctt tttgtttact tttagtagga
    11821 ggcaagagct gtagatagaa ctcaagttca tcaaagcgac ttaaggccct aataaaagat
    11881 aaagcaaacg acggatagaa aaaagcccac acacagaata tacttccgtg tgagggcgtt
    11941 ggtaacgcgg tgccacctca attataaagg gactatccct ttacatctct gccttgttta
    12001 acaacaagct gcactgtaag gtgtgcgcac cgaattttca ttgtttcaaa ttcattttca
    12061 aaatcagccc actttcacta cttccaacca cctattcaca atcaccacag gctccctgaa
    12121 gatcaaaaat agttactttt ctgatttgtt gaacttattt taatactttg ttttttcttt
    12181 gtcaagactt ttttacgatt tttttgaaaa tatcattcga atgtgaccat gtcttcctta
    12241 gatcgaacat gaacatgtcc cacttgttag aaattggatc caactcaata gaaactgaat
    12301 ggaggctaaa cagaacttat tttagaacac tccatctttt ccactaggat tttcaagaat
    12361 taaacaatac tagaaactct gtctcctaac aaatttagga gaaacttcaa cagatgtgac
    12421 actttcccct ttaataatcg ctaaaacacc ttctatcatt tctttagcca atttaacata
    12481 attgggagca attgtagaca aagctggagt ataatactga gaaataggaa tattatcaaa
    12541 tccaatgata gaaatatcat ctggaataag aattcctttc tcatagcacg cacgaatcaa
    12601 gccctgaacc ttttcatctc ctgaaacaaa aataatgtcc ggataatttt ggctagtcaa
    12661 gtgctgcatt gcataagaat aaactgaatc aattgtagat aagccataaa tgacttttaa
    12721 atccataaag taatttttat cattcagaaa agaacgcaca cctctttcac gatccttatt
    12781 aacatgggat tctcctccca taagcaacca catattttta aatttttctt cagttacagc
    12841 tttcatcata tcataagtag cttgaaaatt attattagat acatagacta ctccagacgt
    12901 ttgagattca ccgaaaacaa gaaaaggcat atggttcttc tttaaatact gaattctgat
    12961 atcatctaca ctttcataaa aaacaataac accatctact aggctacctg tgcttgatat
    13021 aattgaatta ctaattgtat cctcctctcc aaagtactca actatagcat taacaccaaa
    13081 ttctttacac gtccgtaaca ctttatctaa cagcgtatga aaccaaatta aaggaaaaga
    13141 gtcgattttt tttacagaaa tcaatatatt tatagcttct tttttagtta aattttttgc
    13201 atacgcattt ggaatatacg acaattcctc tataactttt tgaatcgctt gataagtttc
    13261 ttctttaaca tttactccac cattaataac tcgtgaaact gtttttggag aaaaacctga
    13321 taaacgtgca atatcataaa tagttacctt tttcccattt atatttttca tttcagtcct
    13381 ccattacg
//
LOCUS       DBERMR010000054        12876 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.054, whole genome shotgun sequence.
ACCESSION   DBERMR010000054 DBERMR010000000
VERSION     DBERMR010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12876
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.054"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..4151
                     /locus_tag="ACL8A1_001766"
     CDS             <1..4151
                     /locus_tag="ACL8A1_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ZmpA/ZmpB/ZmpC family metallo-endopeptidase"
                     /protein_id="HGS1202491.1"
                     /translation="PDTVVSDKGEPKQVAPLPEYTGPQVSAIVEPEQVAPLPEYTGVQ
                     AGSIVEPEKVEAPKEYTGKIEQPSAEDTKPENGASSTNGESERPKDKIKEEKQVDKKL
                     ELRNVSNVELYTVENNKYRHITAVDGALDSSLKYFMKVKSENFKDIMLPVTKIESTTK
                     NNKEVYKIVAHAENLIQHENNVISNDYTYYLPKTQQSETGVYTSFKNLVDAMNSDPNG
                     TFHLGATMDAREVELPDDQESYVKNEFYGKLIGKNNGKYYAIYNLKKPLFKTLNTATI
                     QNLSIKEANVSSKEDAATISKEAKYNTLIDNVHSDGIIAGERGIGGLVSKVDNSRISN
                     SSFTGRITNTYDTTAGYEIGGLVGKLSGSLASIEKSIASIDIASNAKSGDQIVGGIAG
                     VVEKSATIKYSYVEGNVNNVRHFGKVGGVAGNLWDRDSQDVSKSGKLSYVLSDVNVTN
                     GNAIAGYNFNGIKTIETYSNKNNKVVNVVQEDDEVVTKDSDVQRGTVLDADKVKEKKV
                     ELVSKHSTKVEDFDFTSRYNTNYNEVTGYQQSREQVYKNIEKLLPFYNRETIVKYGNL
                     VEDNSDLFTKKLLSVVPMKNNEVITDINKNKQEINKLLLHFEGNKSRVLNIAYKNDFS
                     KVAEYDIANTKLMYTPNTMLHDYNNIVKTILNDLKSVQYSSADVRKVLDISGNIKLTE
                     LYLGEQFEKTKANIEDSLSKLLTADAAIVENNNKVIDNYVIEKIKNNKEALLLGLTYL
                     ERWYNFNYGETNAKDLIMYHLDFFGKSNSSALDNVIELGKSGFNNLLAKNNVITYNVL
                     LAKNYGTESLFKALEGYRKVFLPTISNNEWFKKQTKAYIVEEKSTIEEGREKQGKEGT
                     KYSIGVYDRLTNPSWKYQSMVLPLLTLPEEKTVFMIANISTIGFGAYDRYRSSEYPKG
                     EKLNKFVEDNAKEAAKRFRDHYDYWYKILDNDNKEKLYRSILVYDAFKFGTDKDKDKV
                     THQATFETDHPAIKYFFGPAGNNVVHNGHGAYATGDAFYYMAYRMLDKDGAVTYTHEM
                     THNSDREIYLGGYGRRSGLGPEFYAKGLLQAPDHPYDPTITINSVLKYEDSENSTRLQ
                     VADPTQRFNSAEDLHNYMHNMFDVIYMLEYLEGKAVANLETNQKYELLRKIENKFDLD
                     QDGNNVYATNVVRRLTMDEVNKLNSFDSLIENDIITSRGYKDQEYKRNGYYTIDLFSP
                     IYSALSGEKGTPGDLMGRRIAFELLAAKGYKEGMVPYISNQYEKDAKAAGSKINSYGK
                     EVGLVTDELVLEKVFNGQYKTWTQFKKDMYKEREKQFSKLNRVNFINPNNPLSRQRNV
                     SVTDIGVLERMIVEAVRDDAQDDVAKFYPETNSRVLKLKKAIYKAYLDQTNDFRSSIF
                     ENKK"
     gene            4195..9444
                     /gene="zmpD"
                     /locus_tag="ACL8A1_001767"
     CDS             4195..9444
                     /gene="zmpD"
                     /locus_tag="ACL8A1_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease D"
                     /protein_id="HGS1202492.1"
                     /translation="MSLLKKDKFSIRKIKGIVGSVFLGSLLFAPSVIGASTYHYLDYS
                     SLTQTERDQLKQGRPDESKESYALDYEKDALPNTGSSQSIMTALGLLAIGSLIVIITK
                     DNRNKKIATFLIVGATGLVTLSTASALNLNANIHESGRDGVLQISGYRYVGYLELDDK
                     TVSSVSPASTVSPVEQPKVVTEKGEPEVQPALPEAVVTDKGEPEVQPTLPEAVVTDKG
                     EPEVHEKPDYTQPIGANLVEPEVHEKLAYTEPVGTTGVDENGNLIEPPVNDIPEYTEP
                     ISTVSEVASEREELPSLHTDIRTETIPKTTIEESDPSKFIGDDSVKEVGEDGERQIVT
                     SYEELHGKKISEPVETVTILKEMKPKILVKGTKENPKEKTVPVLTLTKVTEDAMNRSA
                     NLNYELDNKDNAEISSIIAEIKDGDTVVKKVDLSKEKLTDAVQNLDLFKDYKIATTMI
                     YDRGQGSETSKLDEKTLRLELKKVEIKNISSTNLVKVNDDGTEIPSDFMSEKPSDEDV
                     KKMYLKITSRDNKVTRLAVDKIELVTEKEKELYKITASAQDLIQHVDPSKTRNEYIHY
                     IEKPVPKVNNVYYNFNELVRDMQEHPNDEFKLGADLNATNVSAFGKSYVTKDFKGKLL
                     SDGDNHYTIHNLSRPLFGNVIGGTIKNINLGNVDINMPWANQVAAVANIIKGGTTIEN
                     VKVKGNIVGKDWVSGFIDKIDNQGTLRNVAFIGNVTSVGDGGQFLTGIVGENWKGLVE
                     RAYVNANLIGKKAKAAGIAYWTQNEGNNNTVRQEGAIKKSIAKGTIQVTEAIESGGVV
                     GSMKHHGSVEDSVSMMKVPNGEIFYGSSDIDYDDGYWTGDNVRRNYVVIGVSDGHSSY
                     QRSKDKNRIRPISEEEAKSKIEATGITADKYEINEPVVNRLNRLTRREDEYKSTQDYK
                     VDRDLAYRNIEKLQPFYNKEWIVNQGNKLAEDSNLAKKEVLSVTGMKDGQFVTDLSDI
                     DHVMIHYADKTKEIKAVHQKESKVAQVREYSIDGLDDIVYTPNMVDKNRDQLIKDIKD
                     RLATVELISPEVRALMDKRDTSRDPNANSDERKNGYIRDLYFEESFSETKANLDKLVK
                     SLIENADHQLNSDEAAMKALVKKVDENKAKIVMALTYLNRYYDIKYGDMTIKNLMMFK
                     PDFYGKSVDLLDFLIRIGSSERNIKGDRTLDAYRDMIGGTIGKSELHGFLDYNMRLFT
                     NDTDLNDWFIHAAKNVYIVEPKTTNPDFVNKRHRAFDGLNNGVHNRMILPLLTLKNAH
                     MFLISTYNTMAYSSFEKYGKYTEAEREAFKDKIKEVAHAQQTYLDFWSRLALPSVRDQ
                     LLKSQNRVPTPVWDNQNYHNVEGVNRMGYDKNNKPIAPIRELYGPTWRYHTTNWYMGA
                     MASIFQDPNNNDQVYFMGTNMISPFGISAFTHETTHVNDRMLYFGGHRHRQGTDVEAY
                     AQGMLQTPDKSGNGEYGALGLNMAYHRENDGDQWYNYDPDKLKTREDIDRYMRNYNDA
                     LMMLDHLEADAVIPKLHGNISRWFKKMDRQYRKNGELHQFDKVRELTEDEKKKIVINN
                     IDDLVNNNLMTKHGAPSDRTYNPEDFDSAYVNINMMTGIYGGNTSQGAPGAASFKHNT
                     FRMWGYFGYENGFISYASSKYQGEADKTNKKLLGDDFIIKKVSKDKFNNLEEWKKQYF
                     KDVKSKAEKGFTAIEIDGRQITNYAQLKTLFAEAVQKDIDGMSDPKIKDHFKNTVDLK
                     SKVFKALLKNTDGFFNKLFKEDI"
     gene            9690..10541
                     /gene="ylqF"
                     /locus_tag="ACL8A1_001768"
     CDS             9690..10541
                     /gene="ylqF"
                     /locus_tag="ACL8A1_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HGS1202493.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWCQYFESQEIQTLAVNSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            10528..11307
                     /locus_tag="ACL8A1_001769"
     CDS             10528..11307
                     /locus_tag="ACL8A1_001769"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HGS1202494.1"
                     /translation="MATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            11323..12873
                     /locus_tag="ACL8A1_001770"
     CDS             11323..12873
                     /locus_tag="ACL8A1_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HGS1202495.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHMIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALIGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
BASE COUNT         4582 a         1986 c         2578 g         3730 t
ORIGIN      
        1 cgccagatac tgtggtaagt gataaaggtg aacctaaaca ggtagcaccg cttccagaat
       61 atacaggacc acaagtcagc gctattgttg aaccagagca ggtagcacca ctaccagaat
      121 acacaggggt acaagccgga tcgatagtcg agccagagaa agtagaagca ccgaaagaat
      181 atactggtaa aatcgaacag cctagtgcag aagatactaa acctgaaaat ggggcaagct
      241 ctacaaatgg ggaatcggaa cgtcctaaag ataaaattaa agaagaaaaa caagtcgaca
      301 agaaactaga acttagaaat gtttcaaacg tggaattata tacggtagaa aataataaat
      361 atcgacatat tacggctgta gatggtgctt tagatagttc attgaagtat ttcatgaagg
      421 taaaatcaga aaattttaaa gatataatgt tgcctgttac taaaattgaa agtacaacaa
      481 aaaacaataa agaagtatac aaaattgtgg ctcatgcaga aaatctaatt caacacgaaa
      541 acaatgttat ttcgaatgac tatacatatt atttaccaaa aactcagcaa agtgaaactg
      601 gcgtctatac atcatttaaa aatttagttg atgcaatgaa tagtgaccct aatggaacat
      661 tccatttagg tgcaactatg gatgctcgtg aagttgaact tccggatgat caagaaagtt
      721 atgtaaaaaa tgaattttac ggaaaattaa taggtaaaaa taatggtaag tattatgcta
      781 tttataactt gaaaaaacct ttatttaaaa ctctaaacac cgctactatt cagaatcttt
      841 caataaaaga agcgaatgtt tcttctaaag aagatgctgc tacaatatcg aaagaagcaa
      901 aatacaatac tcttatagat aatgttcatt ctgatggtat tatagctggt gaacgtggaa
      961 ttggtggatt ggtttcaaaa gtagataact cgagaatttc taatagtagt tttacaggca
     1021 gaataactaa cacatatgat acaactgcag ggtatgaaat tggtggttta gtaggtaaac
     1081 tttctggttc gcttgcttct atagagaaat caatagcatc gattgacata gcctctaatg
     1141 caaaatcagg agatcaaatt gttggtggta tagccggagt tgttgagaaa agtgcgacaa
     1201 ttaagtatag ctatgttgaa ggaaatgtta acaatgtgcg acactttggt aaagttgggg
     1261 gagttgcagg taatctttgg gatagagatt cacaagatgt aagtaaatca ggtaaactat
     1321 catatgtttt aagtgatgtc aatgttacga atggtaatgc tatagctggc tataatttca
     1381 atggaataaa aacgattgaa acatatagca ataaaaataa taaggtagta aacgtagtac
     1441 aagaagatga tgaggttgtg actaaagatt cagatgtaca aagaggtacg gtattagatg
     1501 ctgataaagt aaaagagaaa aaggtagaat tggtttctaa acacagcact aaagtagaag
     1561 attttgattt tacttcaaga tacaatacta attacaatga agtaacaggt taccaacagt
     1621 cgagagaaca agtatataaa aatattgaaa aattacttcc tttctataac agagaaacaa
     1681 tagttaagta tggtaattta gtagaggata acagtgactt atttactaaa aaacttctat
     1741 cagttgttcc tatgaagaac aatgaagtaa taactgatat taataaaaat aaacaagaaa
     1801 taaacaaatt gttattacat tttgaaggta acaaatcacg agtattaaat attgcatata
     1861 agaatgattt ctctaaagtt gctgagtatg atatagctaa tacaaaatta atgtatacac
     1921 ctaatacaat gctgcatgat tataataata ttgtaaaaac tattttaaat gatctgaaat
     1981 ctgttcaata tagttcagct gacgttagaa aagtattaga tatttctggt aatataaaat
     2041 taacagagtt atatttaggt gaacaatttg aaaaaacaaa agcaaatata gaagacagtt
     2101 tatcaaaact acttactgca gatgctgcaa tagtggaaaa taacaataag gtaatagata
     2161 attatgtaat tgagaaaata aaaaataata aggaagcctt attgttagga ttaacgtatt
     2221 tagaacgctg gtataatttt aattatggag aaaccaatgc taaagattta ataatgtacc
     2281 acttggattt ctttggtaaa tcaaatagtt ctgcattaga taatgttatt gagttaggta
     2341 aatctggatt taacaattta ttagcaaaaa ataatgtaat tacttataat gtattattag
     2401 cgaaaaatta tggaactgaa agtttattta aagcattaga aggatataga aaagtattct
     2461 taccaacaat ttctaacaat gagtggttta aaaaacaaac aaaagcatat attgtagaag
     2521 aaaaatctac aattgaagag ggaagagaaa aacaaggaaa agagggaact aaatattcta
     2581 ttggggtata tgatagatta actaatccat cttggaaata tcaaagtatg gtcttaccac
     2641 tattaacttt acctgaagaa aaaacagtgt ttatgatagc gaatatttct actattggat
     2701 ttggagccta tgacagatat agaagttcag aatatcctaa gggagaaaag ttaaacaaat
     2761 ttgtagaaga taacgccaaa gaagcggcta aacgatttag agatcattat gattattggt
     2821 ataagatatt agataacgat aataaagaaa aattatatag aagtattcta gtatatgatg
     2881 ctttcaaatt tggtactgac aaagataaag acaaagtaac tcatcaagct acttttgaaa
     2941 ctgatcatcc agctataaaa tacttctttg gacctgcggg aaataatgtt gttcataatg
     3001 gacacggagc atatgctacc ggagatgcat tctattatat ggcatatcgt atgttggata
     3061 aagatggagc tgtaacatat actcatgaaa tgacacataa ctcagataga gagatttatc
     3121 ttggaggata tggaagaaga agcggattag gacctgagtt ttatgcaaaa ggattattac
     3181 aagctccgga tcatccatat gatccaacaa taacaattaa ctctgtcttg aaatatgagg
     3241 attcagaaaa ttcaacaaga cttcaagtag ctgatccaac tcaacgattt aatagtgcag
     3301 aagacctaca taattatatg cataatatgt ttgatgtaat ttatatgtta gaatatttag
     3361 aaggaaaagc tgtagctaat ttagaaacaa atcaaaaata tgagttacta agaaaaattg
     3421 aaaataaatt tgatcttgat caagacggta ataatgtata cgcgacaaat gttgttcgca
     3481 gattaacgat ggatgaagtt aataaattaa attcttttga tagtttaatt gaaaatgata
     3541 ttattactag cagagggtac aaagaccaag aatataagag aaatggttat tatacaattg
     3601 atttattttc accgatttat tcagctctta gtggagagaa agggactcct ggtgatttaa
     3661 tgggacgccg tatagcattc gaattattag ctgcaaaagg atataaagag ggcatggtac
     3721 cttatatctc aaatcaatat gaaaaagatg caaaagcggc gggaagtaag attaattctt
     3781 atggtaaaga agtaggttta gttacagatg aattagtact tgaaaaagta tttaatggtc
     3841 aatataaaac ttggacacaa ttcaagaagg atatgtataa ggaaagagaa aaacaattca
     3901 gtaaactaaa tagagttaac tttattaatc ctaataatcc attgagtcgt caacgaaatg
     3961 tatcggttac ggatataggt gtactagaga gaatgattgt cgaagcagtg agagatgatg
     4021 cgcaagatga cgtagcaaaa ttttacccag aaacaaatag tagagttctt aaactgaaaa
     4081 aagcaatata taaagcttat cttgaccaaa caaatgattt tagaagttca atttttgaga
     4141 ataaaaaata gtgtttacta ttaggaaata aaaagtgaat gtggaggatt agaattgtca
     4201 ttattaaaaa aagataaatt ttccattcgg aaaataaagg gtattgttgg ttcggtattc
     4261 ttggggagtc ttttatttgc accgtcagtt attggtgcat cgacttatca ttacttagat
     4321 tatagtagtt tgacacaaac tgaacgtgat caactcaaac aaggtagacc tgatgaatcc
     4381 aaagagtcat atgctttgga ttatgaaaaa gacgcactac caaacacagg cagttctcaa
     4441 tctattatga ctgcattggg tttgttagct atcgggagtc ttattgtgat tatcacaaaa
     4501 gataatagaa ataaaaaaat agctacattt ttgattgtag gggcgacagg tttagttaca
     4561 ctatcaactg cttcagcact taatttgaat gctaacatcc atgagtctgg acgtgatggt
     4621 gtgttgcaaa tttctggtta cagatatgtt ggttacttgg agcttgatga taaaactgtt
     4681 tcgtcggttt ctccagcatc aactgtttcg ccagttgagc aacccaaagt tgtaacagag
     4741 aaaggggaac cggaggttca accagcctta cccgaagctg ttgtgactga caaaggcgaa
     4801 ccggaagtac aaccgacatt accagaagct gttgtgactg acaaaggcga accagaagtt
     4861 catgagaagc ctgactatac gcaaccaata ggtgcaaacc ttgttgagcc tgaagttcat
     4921 gagaaactag catacactga accagtaggt acaacaggag tggatgaaaa tggaaacttg
     4981 attgaaccac ctgttaacga cattccagag tatactgaac caatatctac agtttcagaa
     5041 gttgcatcag aaagagaaga gttgccttct ttacataccg atattcgtac agagactatt
     5101 cctaaaacca ctatagaaga atctgatcct agcaaattta taggcgatga ttctgttaaa
     5161 gaagttggtg aggatggcga acgtcaaatt gttactagct atgaagagtt acatggcaaa
     5221 aaaattagtg aaccagttga aacggtaaca attttgaaag aaatgaaacc taaaattctt
     5281 gtaaaaggta caaaagaaaa tccaaaagaa aaaacagttc ccgttttgac cttgaccaaa
     5341 gtaacagaag atgctatgaa tcgttctgcc aacttaaatt atgagcttga taataaagat
     5401 aatgctgaaa tatcaagtat tatagctgaa attaaagatg gtgatacagt agttaaaaag
     5461 gttgatttat ctaaagaaaa attaactgac gctgtacaaa atttggattt gtttaaagat
     5521 tataagattg caacaacaat gatttatgat cgtggtcaag gttctgaaac gtctaaactg
     5581 gatgaaaaaa ctttgcgttt ggagttgaaa aaggtcgaaa ttaaaaatat ttcatctact
     5641 aatttagtga aggtaaatga tgatggtact gaaataccta gtgatttcat gagcgaaaaa
     5701 ccttctgatg aagatgtgaa gaaaatgtac ttaaaaatca ctagtcgtga taataaagta
     5761 actcgtttag cagttgataa gattgagtta gtaactgaaa aagaaaaaga actttataaa
     5821 attacagcaa gcgctcaaga tttaattcaa catgtcgatc catctaagac tagaaatgaa
     5881 tacattcatt acattgaaaa accagttcca aaagtcaata atgtatatta caatttcaat
     5941 gaacttgtga gagatatgca agaacatcca aatgacgagt ttaaattggg tgctgacctt
     6001 aatgccacaa atgtatcagc atttggtaaa tcatatgtta cgaaagattt caaaggtaag
     6061 ctcttgagtg atggtgataa tcactatacc attcataact tgtcacgtcc gttatttggc
     6121 aatgttattg gtggaacgat taagaatatt aatcttggta atgttgacat aaatatgcca
     6181 tgggcgaacc aagttgcagc tgtagcgaat attattaaag gtggaactac cattgaaaat
     6241 gttaaggtta aaggtaatat agtaggtaaa gattgggtat ctggatttat tgataagatt
     6301 gataaccaag gtacactcag aaatgtggcc tttatcggta acgtaacttc tgtcggcgat
     6361 ggtggacaat tcttgactgg tatcgttggt gagaactgga aaggactcgt cgaacgcgcg
     6421 tatgttaacg caaatcttat tggcaagaaa gccaaggctg cgggcattgc ttattggaca
     6481 caaaatgaag gaaataacaa tactgttcgt caggaaggag caatcaaaaa atctattgct
     6541 aagggaacaa ttcaagtaac tgaggcgatt gaaagtggtg gtgttgtagg aagcatgaag
     6601 caccacggtt ctgtcgaaga ttcagtttcc atgatgaagg tacctaatgg tgaaattttc
     6661 tatggttcta gtgatattga ttatgatgat ggctactgga caggagataa tgtaagacga
     6721 aattatgttg taataggtgt tagtgatggg cattctagct accagcgttc aaaagataaa
     6781 aatcgtatca gacctatttc tgaggaagag gcaaaatcta aaattgaagc tacaggaatt
     6841 accgcagata aatacgaaat caatgaacct gtggtcaatc gcttaaatcg tttaaccaga
     6901 agagaagatg aatataagtc aacccaagac tataaagtag atcgagatct ggcttatcgt
     6961 aatattgaga aactgcaacc attttataac aaagaatgga ttgtcaatca aggtaacaaa
     7021 cttgcagaag attcaaactt agctaagaaa gaagtccttt ctgttacggg tatgaaggat
     7081 ggtcaattcg tgaccgattt atcagatatt gatcatgtga tgattcacta tgcagataag
     7141 actaaagaaa tcaaagctgt tcatcaaaaa gagtctaaag tagcgcaagt tcgtgagtat
     7201 agtatcgatg gattagatga cattgtttat acaccaaaca tggttgataa aaatcgtgat
     7261 caattgatta aagacattaa agataggtta gcaactgttg aattaatttc tcccgaagtc
     7321 cgtgctttga tggataaacg agatacatct agagatccta atgctaattc ggatgagcgt
     7381 aagaatggtt atatccgaga cctttatttc gaagaaagtt tttctgaaac gaaagctaat
     7441 cttgataaat tggtcaaatc tttgattgaa aatgctgacc atcaattaaa tagtgacgaa
     7501 gcagctatga aagcacttgt taagaaggtg gatgaaaata aagctaaaat cgtgatggct
     7561 cttacttacc ttaatcgtta ctatgatata aagtatgggg atatgactat caaaaacttg
     7621 atgatgttta aacctgattt ctatggtaag tcagttgacc ttcttgactt cttgattcga
     7681 attggatcaa gtgagcgaaa tatcaagggt gacagaactt tggatgctta tcgtgatatg
     7741 attggtggaa ctattggtaa gtctgaatta catggcttct tagattacaa catgcgtctc
     7801 ttcactaacg atactgattt aaatgattgg tttatccatg cggctaagaa cgtatacatt
     7861 gtggagccta aaacaacgaa cccagatttt gttaataaac gacatcgtgc atttgatggt
     7921 ttgaataatg gtgttcataa tcgtatgatc ctacctctat taactcttaa gaatgcacat
     7981 atgttcttga tttcgactta caatacaatg gcttatagct cttttgaaaa atatggtaag
     8041 tatacagaag ctgaacgaga agcatttaaa gataaaatta aagaagttgc tcacgcacaa
     8101 caaacttatc ttgacttctg gtcgcgttta gcattgccaa gcgtacgtga ccaattactc
     8161 aagagtcaaa atagggttcc aacacctgtt tgggataacc aaaactatca taacgtcgaa
     8221 ggtgtaaatc gtatgggtta tgataagaat aataaaccaa ttgcacctat tcgtgaatta
     8281 tatggaccta cttggcgtta tcatactaca aattggtaca tgggagctat ggcatctatt
     8341 ttccaagatc ccaataataa tgaccaagtt tatttcatgg gaacaaatat gattagtccg
     8401 tttggtattt cagcctttac tcacgaaaca acacacgtaa acgatagaat gctttacttc
     8461 ggtgggcata gacaccgtca aggtacagac gttgaagcct atgcgcaagg tatgttgcag
     8521 acacctgata aatctggtaa cggtgaatat ggtgctcttg gtcttaacat ggcttatcat
     8581 cgtgaaaatg acggtgacca atggtataat tatgatccag ataaattgaa aactcgtgaa
     8641 gatatcgacc gttatatgag aaattataat gatgctctta tgatgttaga ccatcttgag
     8701 gctgatgctg ttatccctaa attacatggt aatattagtc gatggttcaa gaaaatggat
     8761 agacaatatc gcaaaaatgg agagctacat caatttgata aagttcgtga attaactgaa
     8821 gatgagaaga agaaaattgt tatcaacaac attgatgatt tagtcaacaa taatttgatg
     8881 acaaaacatg gcgctcctag tgacagaaca tataatccag aagattttga tagtgcttac
     8941 gtaaatatta atatgatgac cggtatctat ggtggtaata ctagccaagg agcacctgga
     9001 gctgcatcat tcaagcataa tactttccgt atgtggggct attttggtta tgaaaatgga
     9061 ttcattagtt atgcatctag caaatatcaa ggcgaagctg acaaaaccaa taaaaaatta
     9121 ttgggtgatg atttcattat caagaaagta tctaaggata aattcaataa tcttgaagag
     9181 tggaagaaac aatacttcaa agatgttaag tcaaaagcag aaaaaggatt tactgctatt
     9241 gaaattgatg gtcgtcaaat tacaaattat gctcagttga aaactttatt tgctgaagct
     9301 gttcaaaaag atattgatgg tatgtcagat ccgaaaatta aagatcattt caaaaataca
     9361 gtagacttga aatccaaagt attcaaagcc cttcttaaaa acacagacgg tttctttaat
     9421 aaactgttta aagaagacat ttaaatgttt aaaatataaa gaggacagtt ttttgctccc
     9481 tctgaaaagt catcatttga tggctttttt ctatataggg taaaagatag ggtaaaaggc
     9541 tatcatcgga caaaataaag aaggcatgat ataatataaa gtagattttt atatcataaa
     9601 acaagaactg tttggacatc attcattttt agattgagat ctcttagttc tagattaaca
     9661 gcttcagagc tacgaaagga aaaaactcaa tggctactat tcaatggttt cctggtcaca
     9721 tgtctaaagc gcgtcgacag gtgcaggaaa atttaaaatt tgttgatttt gtgacgattt
     9781 tagtagatgc acgcttgcct ctatctagtc aaaatcctat gttgaccaag attgttggtg
     9841 ataaaccaaa actcttgatt ttaaacaagg ccgacttggc tgatccagca atgaccaagg
     9901 aatggtgtca gtattttgaa tcacaagaaa tccagacgct agctgtcaac tccaaagagc
     9961 aagtgactgt aaaagttgta acagatgcgg ccaagaagct catggctgat aagattgctc
    10021 gccagaaaga acgtgggatt cagattgaaa ccttgcgtac catgattatc gggattccaa
    10081 acgctggtaa atccactctg atgaaccgtt tggctggtaa aaagattgct gttgttggaa
    10141 acaagccagg ggtcacaaaa ggtcaacaat ggcttaaaac caataaagat ctggaaatct
    10201 tggatacacc ggggattctc tggcctaagt ttgaggatga aactgttgca cttaagttgg
    10261 cattgactgg agctatcaag gatcagttgc ttcctatgga tgaggttacc atttttggta
    10321 tcaattattt caaagaacat tatccagaaa agctggctga acgcttcaaa caaatgaaaa
    10381 ttgaagaaga agcgcctgtg attattatgg atatgacccg cgccctcggt ttccgtgatg
    10441 actatgaccg tttttacagt ctcttcgtga aggaagtccg tgatggcaaa ctcggtaact
    10501 ataccttaga tacattggaa gacctcgatg gcaacgatta aagaaatcaa agaactcctt
    10561 gtgacagtca aggagttaga aagccctatt tttttagagc ttgaaaagga taatcgctca
    10621 ggagttcaaa aggaaatcag caagcgtaaa agagccattc aagctgaatt agatgaaaat
    10681 ttgcgcttgg aatccatgct ttcttatgaa aaagaacttt ataagcaagg attgacctta
    10741 attgcaggta ttgatgaggt tggtcgtggt cctcttgctg gtcctgtagt cgctgcggcc
    10801 gttattttac ctaaaaattg taagattaaa ggtctcaacg acagcaagaa aattcctaaa
    10861 aagaaacatc tggagatttt ccaagccgtt caagaccaag ccttgtcgat tggaattggt
    10921 atcatagata atcaggtcat cgaccaagtc aacatctatg aagcaaccaa actagccatg
    10981 caagaagcaa tctcccagct cagccctcaa ccagagcacc ttttgattga tgccatgaaa
    11041 ctggacttgc ccatttcaca aacctccatt atcaaaggag atgccaactc cctctctatc
    11101 gcagcagcat ctatagtagc caaggtaaca cgtgatgaat tgatgaaaga atacgatcag
    11161 cagttccctg gctatgattt cgctactaat gcaggatatg gcacagctaa acatctggaa
    11221 ggcctcacaa aactaggagt taccccaatt caccgaacca gctttgaacc cgttaaatca
    11281 ctggttttag gtaaaaaaga aagttaattg aaaggaaata ccatggagga acagtcggaa
    11341 atagtccgtt ctaagaaaga attcgccttt gcatccagca ctatactatc ccaagttggt
    11401 cgaggaatca ttgtcggcct catcgttgga attatcgtcg gatcctttcg tttcttaatt
    11461 gaaaagggct tccacatgat acaaggagtt tatcaagatc aagggtactt agtgcgcaat
    11521 ctttttgtac tggttttgtt ttatatactc atctgttggc tcagtgccaa actaacacgg
    11581 tcagaaaaag atattaaagg ctcaggaatt cctcaagtcg aagccgaact gaaaggcctc
    11641 atgtccctca actggtgggg cattctttgg aaaaaatatg tgctaggtat tctcgctatt
    11701 gccagtggac tcatgctggg tcgagaggga cccagtattc aacttggagc agttggtggt
    11761 aaaggaattg ccaagtggct caaatccagt ccagtagagg aacgttcctt gattgccagt
    11821 ggagctgcag caggtttagc cgcagccttt aatgctccta ttgcagcact tctctttgtt
    11881 gtagaagaag tctatcacca tttttcgcgc tttttctggg tctcaactct agcagccagc
    11941 atcgtagcaa actttgtgtc tctactcatg ttcggtttga caccagtatt ggatatgcca
    12001 gataacattc ctcccatgac cctagatcag tattggatat atctcgtcat gggaattttc
    12061 cttggatttt caggctttct ctatgagaaa gctgtattaa acgttggaag agtttatgac
    12121 ttgattggtc aaaaaatcca tttggataga gcttattatc ccatcttggc ttttatcctt
    12181 atcataccag tcggaatctt cttacctcaa atcattggtg gcggaaatca gcttgtcctt
    12241 tctttaactg aacaaaattt tagtttccaa gttttattag cttacttttt gatccgcttt
    12301 atttggagta tgattagcta tggaagtgga ctgccaggag gaattttcct ccccatttta
    12361 gctcttggtt ctttgcttgg tgccttaatt ggtgttatct gtgtcaatct tggacttgtc
    12421 agtcaagagc aattccctat atttgtcatt ctaggaatga gtggctattt tggagccata
    12481 tcaaaagctc ccttaaccgc tatgatcctc gtaactgaga tggtaggaga tattcgcaac
    12541 ctcatgccac ttggtcttgt cactcttgtt tcttatatta tcatggattt gctcaaaggt
    12601 acgccagtct atgaagccat gctggaaaaa atgcttccag aagaagtatc tagcgaagga
    12661 gaagttacac ttatcgaaat accagtttct gataaaattg ctgggaaaca agttcatgaa
    12721 ctcaacttac cacacaacgt cctcatcaca actcaagtcc ataatggcaa gagccaaaca
    12781 gttaacggct caaccagaat gtatctgggt gatatgattc acctggttat tccaaaaagt
    12841 gaaattggaa aagtcaaaga tttgttgttg tagtac
//
LOCUS       DBERMR010000055        12778 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.055, whole genome shotgun sequence.
ACCESSION   DBERMR010000055 DBERMR010000000
VERSION     DBERMR010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12778
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.055"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..308)
                     /locus_tag="ACL8A1_001771"
     CDS             complement(<1..308)
                     /locus_tag="ACL8A1_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HGS1202496.1"
                     /translation="MQYSCGKININIPDGYGDIKDIVFSAHIIVRYNNGHCGGIDPHI
                     IGLCKKQIRRMSLYPILIIVSRDSKVIDDYKNLDIAYVDCTQCSNNFETALHVKNILK
                     "
     gene            551..1239
                     /locus_tag="ACL8A1_001772"
                     /pseudo
     CDS             551..1239
                     /locus_tag="ACL8A1_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001839528.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1578..2884
                     /locus_tag="ACL8A1_001773"
                     /pseudo
     CDS             1578..2884
                     /locus_tag="ACL8A1_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2924..3934)
                     /gene="tsaD"
                     /locus_tag="ACL8A1_001774"
     CDS             complement(2924..3934)
                     /gene="tsaD"
                     /locus_tag="ACL8A1_001774"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HGS1202497.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLSLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVLDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEVTDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(3924..4361)
                     /gene="rimI"
                     /locus_tag="ACL8A1_001775"
     CDS             complement(3924..4361)
                     /gene="rimI"
                     /locus_tag="ACL8A1_001775"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HGS1202498.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RQSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            complement(4358..5041)
                     /gene="tsaB"
                     /locus_tag="ACL8A1_001776"
     CDS             complement(4358..5041)
                     /gene="tsaB"
                     /locus_tag="ACL8A1_001776"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HGS1202499.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMESLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAYTLNIELVGMSSLLALVPY
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMAEAHLPFERVIELIKGASQVTFVGEVGPF
                     VEQIQKHLPRTDYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            5219..5404
                     /gene="cibA"
                     /locus_tag="ACL8A1_001777"
     CDS             5219..5404
                     /gene="cibA"
                     /locus_tag="ACL8A1_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HGS1202500.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            5407..5559
                     /gene="cibB"
                     /locus_tag="ACL8A1_001778"
     CDS             5407..5559
                     /gene="cibB"
                     /locus_tag="ACL8A1_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HGS1202501.1"
                     /translation="MMKDLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(5882..6097)
                     /locus_tag="ACL8A1_001779"
                     /pseudo
     CDS             complement(5882..6097)
                     /locus_tag="ACL8A1_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282487.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6377..6610
                     /locus_tag="ACL8A1_001780"
     CDS             6377..6610
                     /locus_tag="ACL8A1_001780"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002947111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase subunit epsilon"
                     /protein_id="HGS1202502.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            6612..8291
                     /locus_tag="ACL8A1_001781"
     CDS             6612..8291
                     /locus_tag="ACL8A1_001781"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HGS1202503.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(8228..8467)
                     /locus_tag="ACL8A1_001782"
     CDS             complement(8228..8467)
                     /locus_tag="ACL8A1_001782"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202504.1"
                     /translation="MKIKEQTRKLAVGCSKHCFEVVDRTDEVSSKHRFEVVDRTDEVS
                     NHIYGKAKLTWFEEYYSSSGVRIIGMMIGSRSVFS"
     gene            complement(8671..10584)
                     /gene="mnmG"
                     /locus_tag="ACL8A1_001783"
     CDS             complement(8671..10584)
                     /gene="mnmG"
                     /locus_tag="ACL8A1_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HGS1202505.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDAVPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            complement(10594..11049)
                     /locus_tag="ACL8A1_001784"
     CDS             complement(10594..11049)
                     /locus_tag="ACL8A1_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HGS1202506.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYIIGGA
                     IQVNEKTEDAVVREVKEELGVKAQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDIDKLEGINLVPAFLKTALPDWEGQLRHIHLEE"
     gene            complement(11190..12311)
                     /gene="mnmA"
                     /locus_tag="ACL8A1_001785"
     CDS             complement(11190..12311)
                     /gene="mnmA"
                     /locus_tag="ACL8A1_001785"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HGS1202507.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFSLGHLKKPEVRKLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
BASE COUNT         3663 a         2972 c         2501 g         3642 t
ORIGIN      
        1 aattttaaaa tatttttaac gtgtaaggca gtttcaaaat tatttgaaca ttgagtacaa
       61 tcaacatagg caatatctaa atttttataa tcatcaataa cctttgaatc tctagatacg
      121 attatcaaaa taggatatag agacatcctt ctgatctgct ttttacaaag tccaattata
      181 tgcggatcta tacctccaca atgtccatta ttatacctaa ctataatatg agccgaaaac
      241 actatatcct taatgtctcc atatccatca gggatattaa tatttatttt tccacaacta
      301 tattgcattg taaccatctc cttaaacgac gcattatgat atttgataga gaaattttta
      361 tgaataactc aataatttta tagtaaatca tgcttatatc tcaaagatac ctattttctc
      421 ttgtctcgac cttctccaaa gaattgctat aatactatta caaatacatc tgcactacac
      481 ttcaaatttt agcactgtat aaaaacgttt caatacacta acttcaagaa aacttccact
      541 attaattgaa aaagttgata gagataaatt aaagatctat attgaaactc atcccgatgc
      601 ttatttgact gaaatagctg ctgaattcaa ctgtcctcca acaactattc attacgctct
      661 aaaggctatg ggatatagtc taaaaaagag ccgtacctac tgcgaacaag acccagaaaa
      721 agtaaatcgg ttccttaaag aattgaatca cttaagctac ctgactccta tttatattta
      781 tgagacaggg gttgagacct atttttatct cgaatatgat tgatccttga gcaggcagtt
      841 agtctctctg gaagaagata taattatttg aattaagatc gagacaacgc acaccagaga
      901 ttgcgatact gttatagaag tactaatacc cttttttgtt tcaatatact atggctccga
      961 tgacctataa agatacgatg acgagtgact ttttcgaagc ttgcttccaa aaattcttac
     1021 tacctacttt agatacacca tcccttatca ttatggacaa tgcaaggttt cacagaatga
     1081 acatgtgtaa ggagcagggc atagactgtt accacttcct acctattcac ccgagtataa
     1141 tcccattgag aaaatatggg cttacatcaa aaacatctca gaataatatt gtcaaattac
     1201 gatgcttttc ttgaggcact tttgtcctat tcttgtttca gacgactata ctccgttatt
     1261 gggcagctac ggaacagtcg atgggacgat ggggggacat aaaaaaatcc tccagttttg
     1321 ttttttataa cagtatactg gagaattgac aatctcggta gatacctcgt tatagcgcgg
     1381 ttacttatta ggcagttaca aaacaactgt gaacagaaaa cattccagag tcagacaaga
     1441 ctttggaatg ttttggctct ataatttctg tagtgggtaa tcccacccca ggaattatag
     1501 ggtcgtttct tgtagaaaaa aagccccata tgacctataa tgaaaagcgt ctaaccaact
     1561 cattagaaag gggtcatatg gaacaactta agaataccac agatttgctc ggattggaag
     1621 acaaaaatat caaaatcttg tctgttctga aataccaaac ccatctagtc gttcaggcaa
     1681 agttggattc ccccgctcct ccttgtcctc attgtcaagg gaagatgatc aaatacgact
     1741 tccagaaagc ctctaaaatt ccgcttctcg actgtcaggg tttacccacg gtactgcatc
     1801 tcaaaaagcg ccgctttcag tgcaagaatt gccttaaggt ggtcgtttct caaacatcca
     1861 ttgtcaagaa aaattgccag atttccaaca tggtgagaca aaaaatcgct cagctcctcc
     1921 ttgaaaagca gtctatgact gagattgccc acagattggc ggtctcaact tccaccgtca
     1981 tccgaaaact gagggaattt aagtttgaaa ccgattggac caagttgcca aaagttatga
     2041 gttgggatga gtatagcttc aaaaagagca aaatgagctt cattgcccaa gattttgagt
     2101 ccaaatccat cctcgcaatt ttagacgggc gaactcatgc ggtgattcga aaccatttcc
     2161 aacgctatca gagagaggtt cgggagctgg tcgaggtcat caccatggac atgtacagcc
     2221 cttattatcg gctcgctaag caactctttc caaaggcgaa gattgttctt gaccgcttcc
     2281 acattgtcca acatctgagc cgagctatga accgagtacg aatccaaatc atgaaccaat
     2341 ttgaccgaaa atccttggag tatcgggcgc tcaagcactt ttggaaccct cgctttttcg
     2401 tttctaggct cgggctaaat cagtccactg gattgattta ctacaccagg atagcttcaa
     2461 gctctgtcag aaacgattct atcagcccac gtttcgaatg cacttaaccc atcgggaagt
     2521 acgagataag ctgctttctt actctgaggg attacaggtt cactacgaac tctatcaact
     2581 cctgctcttt cattttcaag agaagaatgc cgaccatttc tttggattga ttgagcaaga
     2641 actgccaacg gttcatccgc tttttcaaac ggtcttttgg acttttttaa gggatagaga
     2701 taagattatc aacgcactta agctgcctta ttccaacgct aaacttgaag cgaccaataa
     2761 tttgattaag gttatcaagc gcaaagcctt tggtttccgg aactttaaca attttaaaaa
     2821 acggattttg atgactttga acatcaaaaa agagagtacg aatttcgtac tctccagatt
     2881 gtagcttttc gcctacccac tacacttgac aaagagccac tctttattcc atggtatcaa
     2941 aggcaagact tggtttggca ttgaggtccc agcctgcgaa gttttctttg ttccactcgc
     3001 tgacgctggc ataggcaatc atacctgcat tgtctccgca gagtcgcaga ggggggatga
     3061 tgaccttgac atctgtaacc tcggctgcta ggcgttctct gagaccttta ttggctgcca
     3121 caccacctgc cacaaccagg gttttaacag gatatttctc caaagccttc ttggttttag
     3181 ccatgagaat atccaagaca gctgcttgga aggaagcaca caaatcttct gtagacaggc
     3241 tttctccctt ttgctcggca ttgtgatgaa gattgataaa ggctgacttc aaaccagaga
     3301 atgaaaactc cagattatct tccttaatca tagcacgagg gaaatcataa atatcctgcc
     3361 cctgatgagc cagctcgtca atctcacgac ctgcaggata ggtcaagccc atgacacgac
     3421 cgaccttgtc ataagcctcc ccaactgcat cgtctcgtgt ctccccaaca atcttgtaat
     3481 cgccagcctc agaaacatag accaactctg tgtgcccacc actgactaaa agggctagca
     3541 agggaaactc caaaggctcc acactctgag ctgccatgag gtgcccagcc atgtgattaa
     3601 caggaatcag tgaaagtccg tgagcccaag caaaggcctt ggcagctgac aaaccaacta
     3661 gcaaggctcc gactaagccc ggtccatagg taaccgcaac agctgtcacg tcctcttcgg
     3721 taatccctgc ttctgccaat gcctcctcga tacaggctgt aatgacctcg acatggtgac
     3781 gactggctac ttcgggcact acgccaccaa aacgtttgtg actctcaatt tgactagcaa
     3841 tgacattgga caagagctca tcgtcgtttt tcaagacggc gacactggtc tcatcacagg
     3901 atgtctcaaa tgctaaaata tatctatcct tcatctattt ctctcttcat gataatggcg
     3961 tcctcgactg ggtcatggta gtaggccttt cgctcagcga taactgccat cttttctttc
     4021 ttgtaaaatg cttgcgctcg ttgatttgac tgtctgactt cgaggaaaat ttccttgtct
     4081 gtcggcaatt gagcaaacaa ggctgacgca attccctgac cctgataagc tcctttgaca
     4141 gcgatttgca ggacttctgc ttcaaaaaga ttctcctgca cagctagaaa tccaatcact
     4201 tctgccccat cataagccaa tgcataccaa gtctggtctt gggacagatc tgcttggatt
     4261 tgctccagag tccaaggact gactaggtaa acagctgcca taacagcgta gatggcttga
     4321 gctaggtcag gctgttgttg aattcgcttg atttctatca taggcgttta atgtaggact
     4381 caccagactc ggtatggttc ttgagccagt tttcctctgc ctcaacacgt ttgaggtaat
     4441 ttggcacaaa atcatgcaag gagtctgctt ccttgtccca ggccaaaaga gctagattag
     4501 ctgcattggg caatgtttct ttgtaatcag tccttggcaa gtgtttttga atctgctcaa
     4561 caaaggggcc aacttctccg acaaaggtta cctgactagc gcccttgatt agctcaatga
     4621 ctcgctcaaa aggcagatgt gcttctgcca tgacaggttt ggcattttca taaaatcctg
     4681 cataaacatt attgcgacgc gcatccatca aggggacaaa caaaccttct tgttggtagg
     4741 gcaccagagc caagagactc gacataccaa ccaactcgat attcagagta taagccagag
     4801 ttttggcagt ggctaccgcg attcgcaagc ctgtgtagct acctggccct tccgccacca
     4861 cgattcggtc caaatccttg ggcgtccagt ccaaactctc catcaaaaaa tcaatggctg
     4921 gcatgagggt aatactgtga tttttcttga tattaatcgt cgtctcagca agaacctgct
     4981 tgtcctctaa aatagccaga gaaagagcct tgctggacgt atcaaaagct aatactttca
     5041 taacacattc ctatcttttt gtctgcttac tattatacta caaaagctgg cacatgggaa
     5101 ttttctttgc ccccagacaa gagtgccctc acttaactaa aaataattaa aaaaaatgct
     5161 cacttttcct tttcttttcc gaatataaaa gtgaacaaga aaaaaggagg aaagttcaat
     5221 gacaaatttt gacattcttg acaatcaatt tttatcctta tctgaaaatg aattatcaga
     5281 tattgatggc ggtctcgctc ccttggttat ctttggagta gcagtatctt ggaaggctat
     5341 tgcaggtgga acagcactta taggttctgg tttggcagct ggttattttt taggaggaga
     5401 ttaactatga tgaaagattt gaacaactat cgtgaaattt ctaataagga attgcaagaa
     5461 atcaagggtg gctttggtgt aggtgttggt atcgctttat ttatggcagg ttataccatt
     5521 ggaaaagacc ttcgtaaaaa gtttggtaag tcatgctaga taagaaacac atttttagaa
     5581 ggataaattt tattgtcttc atctcttaca gtttgctcag cattctcaat gatttgaaca
     5641 ttactactat ccctttacca ttcgatttat ctgtttgtat tgttttattt ttatgcttca
     5701 actctatttt tgagcagaac aatgactccc ataaaaataa taagctttga aaattccatt
     5761 gtcatgtcat gttagaaaaa tgcaaagacc acctcatctt gatagatggg gtggaatttt
     5821 cgtgtcgtaa atctactatc tctacatccc caaacaaaaa accccagcat aagcagggca
     5881 tctaagcatt taatccaaag taaaatacaa accaaacgac ataggtcacg aggaggagaa
     5941 aaagcgagta gagagtcaca aaggtaattt tccacaagaa cttggtttgt catcgttcca
     6001 gtttggcaaa tagaagattt cctccataaa cgcaagcaac aaaaacgata aaagctaaca
     6061 aacgaactcc gatagcaaaa gcaaataagt tatacatagg gcaacctcct tgacttaaaa
     6121 tctatatgga attatgacaa gcaataaatt tcacttccgt tatcaacata atatattttc
     6181 cttgtttttg caaacgctta ccaaagaaat cgtcccctaa ctttctcgtt tccgtctttt
     6241 actaattttt cattttgtgg tataattgaa ataattgtaa cgaatcaagg tcaatctaga
     6301 cacaaaatgg aatgaaatca agcaaatatc tgctaaaagt ttggaataag ctgacctgta
     6361 aatagaaagg aactatatga tttacaaagt tttttatcaa gaaacaaaag aacgtagccc
     6421 acgccgtgaa acaacacgcg cgctttacct agacatcgat gccagctcag aacttgaggg
     6481 ccgtatcact gctcgccaac ttgtcgaaga aaatcgccca gagtacaata tcgaatatat
     6541 cgaactcttg tctgacaaat tgctcgatta cgaaaaagaa actggcgcct tcgaaattac
     6601 ggagttctaa tatggcctac actcttaaac ctgaagaagt cggcgttttt gccatcggtg
     6661 gtctaggaga aatcgggaaa aacacttacg gaattgaata ccaagacgag attatcatcg
     6721 tcgatgctgg gattaaattc ccagaagatg acttgcttgg tatcgactat gtcattcctg
     6781 actactctta catcgtagac aatatcgacc gcgtcaaggc tgttttaatc acacacggac
     6841 acgaggacca cattggtggg attccgttcc tactcaagca agcaaatgtc cctatttatg
     6901 ctggaccgct tgccttggct ttgatccgtg ggaaactcga agaacacggc ctcttgcgca
     6961 acgccaaact ttacgaaatc aaccacaaca ccgagttgac ctttaaaaat ctcaaggcaa
     7021 ctttctttag aacgactcac tctattccag agcctttggg gattgtcatt catactcctc
     7081 aagggaaaat cgtctgtacg ggtgacttta agttcgactt tactccagtt ggagaacctg
     7141 cggacttgca tcgtatggct gcgcttggtg aagaaggcgt gctctgtctc ctgtctgact
     7201 cgacaaatgc ggaagtacca acctttacca actctgaaaa agtcgttggt cagtccatta
     7261 tgaagattat ccaaggtatt gaaggacgta tcatctttgc atcctttgcc tcaaatatct
     7321 tccgtctcca gcaggcaaca gaagctgctg ttaagactgg acgcaagatt gcggtctttg
     7381 gtcgttctat ggaaaaggcc attgtcaacg gaatcgatct tggctacatc aaagctccta
     7441 agggaacctt tatcgagcca aatgaaatca aagattatcc tgcaggagaa gttcttatcc
     7501 tctgtacagg tagtcagggt gagcctatgg cagccctctc tcgtatcgcc aacggaaccc
     7561 accgtcaagt acaattacaa ccaggtgata ccgttatctt ctcttctagt cccatccctg
     7621 gaaacactac tagcgtcaac aagctgatta acatcatttc tgaagctggt gtcgaagtta
     7681 tccacggtaa agtgaacaat atccatacat ctggacacgg tggccagcaa gagcaaaaac
     7741 tcatgctctg cttgattaag ccaaaatact tcatgcctgt ccacggtgaa taccgcatgc
     7801 aaaaagtcca cgctggacta gcagtggata ctggtgttga gaaggacaat atctttatca
     7861 tgagcaatgg cgatgtgctt gcccttactg ctgactcagc tcgtatcgca ggtcatttca
     7921 acgcccaaga tatctatgtc gatggaaatc gtatcggtga aattggcgca gctgtcctca
     7981 aagatcgtcg cgatctatct gaagacggtg tcgttctggc agtcgcaact gttgacttca
     8041 aatcgcagat gattctatct ggtccagaca tcctcagccg aggctttgtc tacatgagag
     8101 agtctggcga cttgattcgc caaagccagc gtatcctctt caatgccatt cgtatcgcac
     8161 tgaaaaataa ggatgctagc gtgcaatctg tcaatggtgc cattgtcaac gctattcgcc
     8221 ccttcctcta tgaaaatacc gaacgtgaac cgatcatcat cccgatgatc ctcacaccag
     8281 atgaagaata atactcttca aaccacgtca gcttcgcctt gccgtatata tggttactga
     8341 cttcgtcagt tctatccaca acctcaaaac ggtgttttga gctgacttcg tcagttctat
     8401 ctacaacctc aaagcagtgc tttgagcaac ctacggctag cttcctagtt tgctctttga
     8461 ttttcattga gtacaaatca acaaaacagc cccgtcttcg gagctgtttt tctctatgct
     8521 ttcttttgag attaaaactc aatgaaaatc agaccagaag gctaggaaaa tgcataactt
     8581 gagttagctc ccatagttcg gggaactatg ggaggttgga gatgaatcaa agccaagctt
     8641 tgaactcatt cgtaagaagc cgacgacgta tcattttgat ttttgaagag ttttagaaat
     8701 actacgattt ttaccttcca gatacaccat caaaatagaa atatctgctg ggtttactcc
     8761 cgaaatacgg ctggcttggc cgatggtttc tggattgatg agtttgaact tctgacgagc
     8821 ttccgtcgca atagaatcga tgtcatccca gtcgatattg gctggaatgc gtttttcttc
     8881 catacgtttc atcttggcaa cctgatccat ggctttggaa atatagcctt cgtacttgat
     8941 ttctgtttca atcaattcga taatcttgtc atccaagtct tctgcagctg gtccgatgaa
     9001 ggccaccaca tcttggtaag aaacttctgg acggcgaagg aattccttgg ctgtcaccgc
     9061 atctgtcaac ggcttgaagc ccatttcctc aaccttagca ttggtttcct tgactggctt
     9121 gagtttgata ctgtctaggc gtttcatctc attatcaaat tgatttttct tgatttcaaa
     9181 acgagcccag cgttcatcat ccacaaggcc aatctcacgg cccatctctg tcaaacgcat
     9241 atcggcattg tcatggcgaa gaatgagacg gtattcagca cgactggtca agagacggta
     9301 aggttcaatg gttcccttgg tcaccaagtc gtcgatcatg accccgatat agccgtcact
     9361 gcgcttcaaa atcaactcag gcttgccttg gatttttaga gccgcattga tacctgcgat
     9421 aatcccttgg cctgctgcct cttcgtaacc tgacgttcca tttgtctgac cagcagtgaa
     9481 gagacctgag attttcttgg tttccaaagt cgcacgcaac tgatgaggca agaccatatc
     9541 atactcaata gcataacctg tccgcatcat ctctgcattt tccaaacctt tgatggaatg
     9601 aaccaagtca cgctggacat cctcaggcag actggttgaa agtccttgaa catagacttc
     9661 ctcagtattg cgcccttctg gctcaaggaa gagttggtga cgttccttgt ccgcaaagcg
     9721 cacaatcttg tcttcaatcg acggacagta acgaggcccc actcccttga ccacacctgt
     9781 aaacataggc gcacggtgga ggttgttttg aataatctca tgactggtac cattggtata
     9841 ggtcaaccag catggcactt gatctttgac ataatcctca tcacgtgaag tgtatgagaa
     9901 atgattaggt actgcgtctc ctggttggat ttctgtcacg tcgtagttga tagaagaggc
     9961 cttgacacgt ggaggggttc ctgtcttgaa acgaccgatt tcgagaccca gttccttgag
    10021 attgtcagct aggttaatag aagccaagct gtggttagga cctgatgagt acttgaggtc
    10081 tccgatgata atttccccac ggagagcagt ccctgtcgtc acaataacag ccttagcagc
    10141 atactcttga tgagtcgctg tacgaacacc gacaactttg ccatcttcca ccaaaatctc
    10201 atcaatcatg gtttgacgaa gggtcagatt ttcttgattt tcaactgtct tgcgcatttc
    10261 cttagagtaa agttccttat cagcctgcgc acgaagggca cgaacggcag gaccctttcc
    10321 tgtgttgagc atcttcatct ggatgtaagt cttgtcaata gtcttggcca tctcgccacc
    10381 gagggcatcg acttcacgta cgacaatccc cttagcagaa ccaccgatag agggattaca
    10441 aggcatgaaa gccagcattt caatattgat ggtcgcaagc aggaccttac agcccatacg
    10501 gctagcggcc aaggaagcct caaccccagc gtgtcccgca ccaattacaa taatatcgta
    10561 ttcttcagta aaatgataag tcatgtttct ctcctattcc tcaagatgaa tgtgtcttag
    10621 ttggccttcc caatctggta gggccgtttt taaaaaggct ggaactagat tgataccctc
    10681 aagcttgtct atgtcaatcc attcacaggg ctgccttttc tcatcttcct gcatggtcaa
    10741 cggggcatct tcaagcaaat ccaccagata atgaaattcg atattgtgat aggaaacgcc
    10801 gtccacttca aaacgatttt caaccacaaa agctagctgc ccagcttgag ctttaacacc
    10861 cagttcttcc ttcacttcac ggactaccgc gtcttccgtt ttttcgttaa cttgaatcgc
    10921 accgccgata atgtaatact tgcccttgtc tttggtgact agaagcttgt gattttggac
    10981 aatcaaggct gtcgcccgaa caccaaaaac cgtattgtct acttttgtcc gaaagtcttg
    11041 ttgagtcatt cttgtccttt cccttaaacg acacaaaaac agtcaaaact acaaagaagt
    11101 gcaggacaaa aaagcctgca acatccatga gctttgacca tcatttctat tgctgttatt
    11161 attgtagcaa attgagaaaa tttgtcaatc taaatgtact gacaaacttg tccatcgcgg
    11221 taagcattgt caatcaaacc gccacctaga cactcttcgc catcgtaaaa gacaactgcc
    11281 tgtcctggtg taatcgcgcg ttgtggttcc gcaaagataa cttctgcctt gtctcctttg
    11341 acatggacgg tcaccttaga gtcaggctga cggtaacgga atttagccgt acattctagc
    11401 gtaaactctt ctggcatttc acgagtaaag tggacttggc tagcctctag gctggttgac
    11461 atgagcgaat catggtagaa accttggcct acatagagaa tattcttgct tagatctttt
    11521 ccgacaacga accaaggggc attgtcacca ccgtgttgcc caccgatacc gagtccgcca
    11581 cgctgaccga ttgtatagta cataagacca gcatgctcgc ccatatcacg accatccaca
    11641 gtcatcatac gaccaggctg agctggcagg tagttgctga gaaagttttt aaagttcttt
    11701 tctccgataa agcaaatccc tgtcgagtct ttcttcttag cagtcgaaag acctgcttct
    11761 tctgctagtt ttcgaacttc aggctttttc aaatgtccta gtgagaacat ggttttttga
    11821 agttgttctt gcgaaagttg gctgaggaaa taggtctgat ccttgccatt gtccacgcca
    11881 cgaagcatgt gaacagtgcc atcctcatca cgcgccactc gagcatagtg cccagtcgct
    11941 acatagtctg cccccaaggt catggcatag tccaaaaagg ccttgaactt gatttccttg
    12001 ttgcacataa cgtccggatt tggcgtgcgc cctgcacggt attccgctag gaaatactca
    12061 aaaacgcggt cccagtactc tttttcaaaa ttgacagagt agtagggaat gccaatctga
    12121 tctgccaccg caaccacatc cttgtaatct tcggtcgccg tacagacgcc gttttcatct
    12181 gtgtcatccc agttcttcat gaagataccg atcacatcgt agccctgctc cttgagcaaa
    12241 agagccgtca ccgacgaatc aacaccacca ctcatcccca cgacaacacg tgttttagag
    12301 ttatcactca tggtaagtct cccatctatt cgtttattca cgattgaagg tcgtgtgtgc
    12361 ttcacgattg aaggtcgctt gagcagtatt cattataaca cgcttggttg gagaagacaa
    12421 ggaagagctt aaaaattgtt ttttaaaact tatagtcaat atacagatta tgtgaatgat
    12481 gtgaatatac cttcttctta tcttcattca aataactata ataaataaaa caacccattt
    12541 tctctaatct attttccaag ttagagaaaa ggagtcgttt ttttaatgac atttcctgaa
    12601 atcataagac aggtacctaa agaatacgaa cataaatcta ttcaatcgaa ccttatcttt
    12661 gtttcaaaat taaaatgtca aatgttctta acatgattta atttaatcgg cttaaaccca
    12721 ttcaccgttt tcattgacag tatatccgtc tacagttgtg ttgactgcaa gggcacct
//
LOCUS       DBERMR010000056        12622 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.056, whole genome shotgun sequence.
ACCESSION   DBERMR010000056 DBERMR010000000
VERSION     DBERMR010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12622
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.056"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(112..549)
                     /locus_tag="ACL8A1_001786"
     CDS             complement(112..549)
                     /locus_tag="ACL8A1_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HGS1202508.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKSYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            complement(634..3267)
                     /gene="polA"
                     /locus_tag="ACL8A1_001787"
     CDS             complement(634..3267)
                     /gene="polA"
                     /locus_tag="ACL8A1_001787"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HGS1202509.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVAEGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIMVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            3523..4430
                     /locus_tag="ACL8A1_001788"
                     /pseudo
     CDS             3523..4430
                     /locus_tag="ACL8A1_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603324.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            4557..4838
                     /locus_tag="ACL8A1_001789"
                     /pseudo
     CDS             4557..4838
                     /locus_tag="ACL8A1_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436408.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4972..5940)
                     /locus_tag="ACL8A1_001790"
     CDS             complement(4972..5940)
                     /locus_tag="ACL8A1_001790"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HGS1202510.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(6085..6899)
                     /locus_tag="ACL8A1_001791"
                     /pseudo
     CDS             complement(6085..6899)
                     /locus_tag="ACL8A1_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
     gene            complement(6924..7421)
                     /locus_tag="ACL8A1_001792"
     CDS             complement(6924..7421)
                     /locus_tag="ACL8A1_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HGS1202511.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(7494..8855)
                     /gene="radA"
                     /locus_tag="ACL8A1_001793"
     CDS             complement(7494..8855)
                     /gene="radA"
                     /locus_tag="ACL8A1_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016893792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HGS1202512.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(8869..9384)
                     /locus_tag="ACL8A1_001794"
     CDS             complement(8869..9384)
                     /locus_tag="ACL8A1_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HGS1202513.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(9386..9829)
                     /locus_tag="ACL8A1_001795"
     CDS             complement(9386..9829)
                     /locus_tag="ACL8A1_001795"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HGS1202514.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            complement(9886..9983)
                     /gene="ffs"
                     /locus_tag="ACL8A1_001796"
     ncRNA           complement(9886..9983)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACL8A1_001796"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(10016..10483)
                     /gene="tadA"
                     /locus_tag="ACL8A1_001797"
     CDS             complement(10016..10483)
                     /gene="tadA"
                     /locus_tag="ACL8A1_001797"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HGS1202515.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYNILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(10691..11977)
                     /locus_tag="ACL8A1_001798"
     CDS             complement(10691..11977)
                     /locus_tag="ACL8A1_001798"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HGS1202516.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(12208..12444)
                     /gene="comW"
                     /locus_tag="ACL8A1_001799"
     CDS             complement(12208..12444)
                     /gene="comW"
                     /locus_tag="ACL8A1_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma(X)-activator ComW"
                     /protein_id="HGS1202517.1"
                     /translation="MLQKIYEQMANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCRKDFIVYHYRVAYRLYLEKLVMNRGFISC"
BASE COUNT         3970 a         3055 c         2127 g         3470 t
ORIGIN      
        1 gtatctacaa cctcaaaacg gtgttttgag cagcccgcgg ctagtttcct agtttgctct
       61 ttgattttca ttgagtataa cacaaaaggt agcccatcag ctaccttttt cttatgcttc
      121 ctcaatcaag cgagtatgtt ctctcttgat acagcgattc atcacgatat catcacatcc
      181 accatcacgc aaaatctctt ttgcttctaa actttcaagt cctagctgtg cccaaaaaat
      241 cttggcatca gctttgagaa aatcacgcgc cacatcgggc agaaattcac tgcgacgata
      301 aacattgaca atatctacag gaaaaggaat ttcagcgagg ctagcataag acttttcacc
      361 caagatttcg ccacctgccg ccttgggatt gactgggatg attttatagc cccgagcctg
      421 catttccttt gtcactcgat tgctggttgt ttcttcacgg tcagacaaac ccaccacagc
      481 aagggtttta ctcgttgcga gatactgacg aatcacgcca tcacttggat tgataaattc
      541 ttgactcata gaaatcctcc tttttcatca gtatagcaca ttttgaaaag gtttgcagaa
      601 ttctactaca aaaaaggagg actagccccc tttttattta gcctcgtacc aggttgcccc
      661 ttcattctca tctgcgataa gaggaacact gagttgaatg gcttcttcca tggtttgttt
      721 caccaatttt ttcatctcta ccaattcaga tttaggcact tcaaggacga tttcatcgtg
      781 cacttgtaac agcatcttag tctgataacc acctgcaacc aaggctttat ccagctgaat
      841 catggcaatc ttgagaatat ctgctgccga accctggata ggtgagttga tagcagttcg
      901 ctccgcaaaa ccacgaatat tgaagttgcg cgaattgata tctggcaact cacggcgacg
      961 cttaaagagg gtctctacat agcccttatc acgcgcctcc cgcaccactt catccatgta
     1021 gtttttaata cctggaaaac gttcaaagta ggtatcaatg taggctttgg cttccttacg
     1081 actaattccc aaattattag acaagccaaa gtctgaaatc ccataaacca ctccaaagtt
     1141 aactgccttg gcattgcgac ggtcatttgc agtcacatta tcaggacgct caatgccaaa
     1201 gacccgcatg gctgtcgaag tatggatatc tgccccctct tggaaggcct taatcaagtg
     1261 ctcatcctta gaaatatgcg ccaaaacgcg caattcaatc tgtgaatagt cagagctgag
     1321 tagcacacta tcctcccact ctggcacaaa agccttccga atcaagcgcc cttgttccaa
     1381 tcgggcagga atattttgca agtttggatc cacactagac aaacgcccgg tctgggtcaa
     1441 atcctgcata tagcgagtat gaatctttcc atcagccaaa atccagtcct gcaagccaat
     1501 tacataagta gattgaatct tagcaatttg acggtaatcc aggattttct taacaatcgg
     1561 agcaatagga gcgagacgct ccaacacatc cactgctgtc gaataacctg tcttggtttt
     1621 cttagtgtat tctagaggaa gtcccaattt ctcaaagaga agcacgccca actgcttagg
     1681 cgagttgaca ttaaactcct caccagccag ctcgtaaatc tcttgagtca gtttttcaat
     1741 gacaagctca ttttcagcct gcatctcaag caaggtctct ttcttgacca taatcccagc
     1801 aatttccatc ttggcaagga caaaagccag aggttgctcc atatcataaa gaagctctaa
     1861 ttgcccattt tcgctgagtt tttcaagtaa aataggctct gtttctacca aaacagcaag
     1921 tttacaagct aagtgttcca agaatttctc acgttcagga atggcctttt taacaccctt
     1981 accgtagaaa gtttcatcat caaccaagta agtctgacca taaagactag cgatggtcgc
     2041 aatttcattg tcctccacag tcgaaaggag gtatttagcc aaacggatgt caaaagcagg
     2101 cgcctgcaaa tccacaccaa aacgttgcaa aagaacttta accttcttaa agtcataaac
     2161 tctcagagat gttttttcta agaaatcctt gaaaatcggg tcttgcaaca gctcaagctt
     2221 gtctgtggca tagagcttat ccccacaaga ccaggcaaat ccaaccaaat tatccgtatg
     2281 gtaattctca ccaaaaagct caaagtggaa gatagactct tcactcagca tatcttgact
     2341 gatttggtca acaatagtaa aatccaaacc ctcagccaca tcagctgacg acacatttaa
     2401 agcctgcttg agctgtttga atcccatctc atcgtaaaat ttcccaagat tttcaacatc
     2461 tggaccacta tagaccaagt cctctaaacc aatcgcaatc ggtgccttgg tatcaatggt
     2521 cgctagtgtt ttagacaaaa aggcctgttc cttgtcatta atgagatttt ccttcatctt
     2581 agaagtcttc attccatcaa tattttcata aatcccctca agcgaaccat gctccagcaa
     2641 gagcttaata cccgtctttt caccgacttt ggtcacccca gggatattat ccgacttatc
     2701 acccatgagc gccttgagat cgataaactg agctggtgtg aggcccattt cttccatgag
     2761 gtaatctggc gtaaaggcct caaactcagc cacacctttc ttggaaattt caaccaccgt
     2821 atgctcatcc gtcagctgaa tcaaatcctt gtccccactg acaatggtaa tatcaaaacc
     2881 atcctgctct gctagcttat ccagcgtccc aatgatgtca tccgcctcat actgagccag
     2941 ctcatagtga cgaatcccca tatgatccag caactcacga atgaaaggaa attgctcacg
     3001 aaactcatca ggagtcttgg cccgaccacc cttatagtcc gcatacatct ctgtccggaa
     3061 ggtcgtcttt cccgcatcaa aagccaccaa aatatgactc ggctcaaccc gctccaacaa
     3121 atgactcaac atcaactgaa aaccataaat cgcattggta tgcaaaccag ccgcattctt
     3181 aaaacggtcc aactgctgat acagcgcaaa aaacgcccga aaagctacag aagacccatc
     3241 aatcaataat aattttttct tatccataca cccattataa aggaaagaat caaaaaatac
     3301 cattgggaag agctagagct agtatttttc aaactttttc cgaataaata gatagagcca
     3361 gagaatttag taaacctaga tttaaaaatg tgctataata tagtatattg aatctataat
     3421 agtacacctt gactactaaa atatttctat aaattaattt gactttcctg atagagttat
     3481 tcacatctta tttcaactca ctatagaagg aggaatagga ggattctcag acatccgggc
     3541 atcagcccaa ctaatgattt gattgctaag aaaatattca gcaatccaga aatcacttgt
     3601 caatttattc gcgatatgct ggacttgcca gcaaaaaatg tgaccatttt ggagggaagc
     3661 gatattcacg tattactctc catgccttac tcggtgcagg atttttatac cagtatagac
     3721 gtcttggcgg agttggataa cggtactcaa gtaattattg agattcaagt ccatcatcag
     3781 aattttttca tcaatcactt gtgggcttac ctgtgcagtc aggttaatca aaatcttgaa
     3841 aaaattcgtc agcgagaagg tgatactcac tagatctaca aacacatcgc tcctgtttac
     3901 gtcattgcta tcgtggatag taattatttc tcagatgacc tggcttttca tagctttagt
     3961 atgcgcgaag acacaacagg tgaggtattg gcgattacca acaatggaca ggaaaaccat
     4021 ctggttaaga tggcattctt ggaattaaaa aatacagaga aaccagcaaa gacaaggttc
     4081 gcaagccatg gttggagttt ttcggcaaca agccctttac ccagcaaccg caacgagcca
     4141 ttacccaagc aaatcaactg ctggactaca agagctggtc cgaggaggac aggaaaatgt
     4201 ttagtcaact acatatgcga gaagaacaag tcttgttagc acaggactat gccttggaaa
     4261 ctgctagggc tgaaggcctt gaacaaggac tagagcgtgg gaaagttgaa ggaagggcag
     4321 aaaggaaact ttttgccttc ctagacatag tacgccaagg tcttctgact tctgaggttg
     4381 ccagccagca attaggtatg tcagtatctg aatttgaggc actgttgtaa aatggctcca
     4441 taatatccat aggggattta cccactatgg atattatgga gcctattttg tgtaaaaaaa
     4501 aggtcccata tgacctataa tgaaaagcga caaaacaact cattagaaag aatcatatgg
     4561 aacaattaca ttttatcaca aaattactag acattaaaga ccctaatgtc cagattttaa
     4621 acatcatcaa taaggataca cacaaggaaa tcatcgccaa actggactac gacgccccat
     4681 cttgccctga gtgcggaaac caattgaaga aatatgactt tcaaaaacct tctaaaattc
     4741 cttatcttga aacgactggt atacctacta gaattctcct tagaaagcgt cgattcaagt
     4801 gctatcactg ttcaaaaatg atggtcgctg aaacttctga tgacgtacag tcatatttct
     4861 tctcttttta ttatatcaca gttttaaatc tagctttact agattcaccg ctactatcta
     4921 tttattcgga aaaaagacga aaaaacctga gaatcatctc aggcttggtc attaaatttt
     4981 tttctcaata tcgaaaagtg gagaaagtgg tcgtttttca tgaatacgta cgatagcatc
     5041 ccctaggaga tgagcgattg aaatctgctc aatcttatca atcaaacgct cttctggcag
     5101 atagatggta tccaaaacaa ccaatttctt aatagctgat ttttggatat tgtccgtagc
     5161 aggaccagaa agaactgggt gcgtacagct tgcatagact tcaacagcac cagcttccgc
     5221 aagagcatct gccgcatgac aaatcgttcc agcggtatca atcatatcat caatcaagat
     5281 acaagtcttg ccttcaacct taccgatgat gttcataact tcactagtat tcatcttatc
     5341 aacgctacga cgtttatcaa taatagcgat agatgttttc aaaaattctg ccaacttacg
     5401 agcacgagtc acccctccat ggtctgggct gacaaccaca tagtcagaac caaccatacc
     5461 acgacgctca aaataatctg caattagagg agcacccatc aaatgatcca caggaatatc
     5521 gaagaatcct tgaatttgcg cagcatgcaa gtcgatggtc aataaacgat ccactccagc
     5581 tacttcaagc atatttgcga caagttttga agtgattggc tcacgcgctc tcgcctttct
     5641 atcctgacgt gcatacccat agtaaggcat gacaacattg acagattctg cactcgcacg
     5701 cttcaaagca tctaccataa tcaaaatttc aagcagattg tcatttacag gcgaactagt
     5761 tgattgtagg ataaagacgt gtttcccacg gattgattct tcaatgttga cctgaatctc
     5821 tccatctgaa aattggcgaa cacttgattt ccccaactct atcccaatct cctgcgccac
     5881 acgttctgcc aattctttat tagaagaaag ggcaaacagc tttaaatcag aaaaagacat
     5941 gatttcctcc ggtatatatg tataacttgt gcttttcaca agattttcca tctaccattg
     6001 tagcgctttt tgcactattt ttcaatcaaa aataaaagaa gagcaccata tttgtaccct
     6061 tgcatcattc ttttgaaaaa tattctaggt catcaactca ttgtgtttct caacaaagca
     6121 ataagcatga taaaaaccat agagagcaat agccgtaacc actggaatcg ctaaaggcaa
     6181 ctctgtttcc aactccacaa aaggagagtt aaacaagaag tgagttccca aggctaaacc
     6241 tagaaaaata aggccctgtt tcttgccaac cttctgtcct ttataggctc tgtaaagcaa
     6301 gtaaacacct actacagcta gacctgaaaa agtccagtga gaggcaattc ctgagatgat
     6361 acgctctaaa attcgcgaaa tagtaaagtc aaagccctct ggcaaatccg tacgaatata
     6421 accaatatcc ttaatcattt ggaatcccaa accggaagca attccaagta aaaacaaaga
     6481 ttttaatttt cgcacaggaa tcaaagccaa aacaaaaaca agtggcaata atttcaaggg
     6541 ttcttctacc aaaggagcca caatagcact ttcaaaggca tttaaaaatg tactatctgg
     6601 gaaaagaacc cccagtaaat catggatata agtattagca aaactagaca accagcctga
     6661 aaggaacatc cctcccaata aagacagaat caaaaccttc tttggcaatt cccatttttc
     6721 ccaatacgga agagaaaata aagagccgga atcatgtaaa agagagctag aaagatagaa
     6781 actcccatta gtccatattc cgcacctgac ctcgaaccgt ccgtatagta gatggtttca
     6841 tactgtaaac caatacatag caataaaata aaaataaata aaatactgct tttcttcata
     6901 cactttcttt ctaaatgaag tatttataat tctacgactg tcatacttcc tgtatcaaca
     6961 ttgtaaatgg caccagagat aatgacatcg tctggtatta ggggagactc gataagcagt
     7021 tgcatatcct cgcgtacact ctcttctata tcttggaagg gcaagaagtc ctggtctgac
     7081 acatctacac ctaattcctc ttttaaatac tcctgaaaag gttcattttc aaaggtctga
     7141 gcaccacagt ctgtatggtg caataccaca atctctcttg tccccatttg ttgctgggaa
     7201 ataactagcg aacgaatcat gtcttcagtc actcgaccac ctgcattccg caagatatga
     7261 gcatccccaa gtgccaaacc tagagcttgc gcaacgtgca aacgtgagtc catacaggtc
     7321 acaatggcta ctctggtttt aggtttaagt ggcagattta actgcccatg tagggcaaca
     7381 taagcctgat tggcttgcat aaactgttca aaatacgaca cgattccctc cttgaaaatt
     7441 tgatagtcaa atatttctcc tatcttatca tttttaagag aatttgtcac ggattatgca
     7501 aagacctttt tcaaaacttc ctgaatcgtt gtcacgccaa tgacctgaat ttccttaggc
     7561 agagtgattc ctgtcaagga attcttaggt acataaatct tagtaaagcc cagtttagca
     7621 gcttcgttga tgcgttgctc aatacgattc acgcgccgaa tctctcctgt caagcccagt
     7681 tctccgacaa aacattcctg aggattagtt ggcttgtctt tgtagctcga agcaatagca
     7741 actgcaacag ccaagtcaat cgcaggttca tccaatttaa caccaccagc agatttgaga
     7801 taggcatcct gattttgcaa gagaagccct gcccgttttt ccaaaacagc cataatcaag
     7861 ctagcacggt taaaatcaag tcctgtcgtc gtacgcttgg catttccaaa catggtcggt
     7921 gttaccaaag cctgaacctc cgccaaaatc ggacgcgtcc cttccatggt tacaacgatg
     7981 gaggaaccag tcgccccatc caaacgctct tctaggaaaa cttgactcgg attgagtacc
     8041 tcaaccaagc cgcccgactg catctcaaaa atcccaatct cattagtgga accaaaacga
     8101 tttttgaccg ctctcaaaat acgaaaggtg tggtgacgct ccccttcaaa gtaaagcacc
     8161 gtatccacca tatgctccaa catacgaggc ccagccaagg ttccttcttt ggtcacatga
     8221 cctacgataa agatggcaat gttattggtc ttggccaact gcatgagttc agcggtcact
     8281 tcacgtacct gagaaacaga cccctgcacc cctgaaatct caggagacat gatggtctgg
     8341 atggaatcaa taatgagaaa gtctggctgg atacgctcca cttctgcacg aacactctgc
     8401 atattggtct ctgcatagag ataaaactca ctatcaatat cacctaagcg ctctgcacgt
     8461 agtttaatct gctgggcaga ctcctcccca ctgacataga gaactgtccc cacttgggac
     8521 aactgggttg agacttgtag gagaagagtt gatttcccaa tcccaggatc cccaccgatg
     8581 aggacgagac ttcctggtac cactccgcct ccaagcacac ggttgaattc ctccatctcc
     8641 gtcttggttc gattgacatt gatggaagtc acctcagcta gtttcatggg cttggttttc
     8701 tcacctgtca aggacacacg cgcattcttg acctcggcaa cctcaacctc ttccacaaaa
     8761 gaagaccaag acccacagtt ggggcaacgt cccagatatt taggggaatt atacccacaa
     8821 ttttgacata caaatgtcgc ttttttcttt gcgatgacaa acctctttct atatctctaa
     8881 ctcacactca atcacttggc aaaaatcaat cttctcattt ggcacaaact ggcgcatgag
     8941 cattcgatga gcaacaacta ccacagtctg atgttctcga tacttagaca tacattctag
     9001 aaaccgagac ttcatttccg tagctgtctc atattgaata ggactattag gaagcaactc
     9061 ccccttgttt tctaaaaaca gtcttctagc tgtttcaaag ttttctattc ctgttttata
     9121 gacctgccat tcatgtaata aaggctctac tcttaaagga agacccgtag cacagaccac
     9181 atacgaagcc gtttctaaag ctcttgtgac tgcagaagat acgattattt cagctgacga
     9241 gagtaaagga tttttgctca atttctggac ttgctgccgt cccatctcag acaagggtgc
     9301 caaatctatc ccaaatccta tataagaacg ctcctctaac tcacggtaat ctggctcccc
     9361 atgacgtaca aagataatct tcattctagt gccctgtcga tccaaatcca ccagttcgaa
     9421 cgccatcagc tgcatctcca tctgcaatta agaaagtagc aaaaacagcc tggacaatac
     9481 gttccccaac ttcaagaaca acctcttgat ctgtgatatt cttcatctgc gcaaaaatat
     9541 gcccttcatt tccaggattt ccataataat ccccatcaat gaccccaact gagttaatta
     9601 aaaccaagcc cttcttacga ggatttgaag aacgatcata gaggtagaga acctcagtcg
     9661 gctgcatata agccttaacc cctgtcggaa ccaagacaat ctctcctggc gcaacaactg
     9721 tacgcacagc aacctttaag tcgtaaccag ccgcatgcgc tgtctcacgc ttgggcaata
     9781 aattttcatc tgtaaaactc gaaaccaatt caaaaccacg aattttcata attttctctt
     9841 ttctattatc atttattcta gattattcta tcttatttat tcggaaaaag cacgaaaaaa
     9901 agagcacaca attcaaatcg cttagggctg ctggattcct cccctgaccc gcttcacgca
     9961 gaactgttgc tccactattt attatatcac attcctattc attttaaaag caaaattatt
    10021 ttttccgtct atttctaaaa aaatcctgca tgatagctgc gcattcatct tccaaaattc
    10081 ctatttcaac ctccacacga tgattgagac gctcatctgt caagatattg tacaaactcc
    10141 cagcagcgcc aaatttctgg tttttagccc catagaccac atttggaatg cgggcaagcc
    10201 caatcgctcc actacacatg acacaaggtt caatggtcac aaaaagtgtg caatccagca
    10261 agcgccagct ctcctcactc aagttcgcat cctctatagc cataatttcc gcatgcataa
    10321 ccgctcgctg taattcctca cgcgcattat gcccacgacc aatgatttcc ccatctttga
    10381 caatcacaca accaattgga atttcatcgt gttcaagagc aatctcagcc tctctcaaag
    10441 cctccctcat aaagacttct ttttcttcaa gtgtataata catctctttt ctcttttctc
    10501 ttttcctact tatcgatttt attattatat catgaatccc aacacaaaaa aagccaccga
    10561 atgcggtgac tctataggga gattattatg aaaaagaaaa gtttaggata tttgttacaa
    10621 caagttagga ggtcttcttg taaccgttta tagtataccc gacctatctt aaacaaatct
    10681 taaaaatctc ttaggaccaa acactttcta aaatatttgt ttgttcacga ccaggaccta
    10741 ctgagaaagt agaaatacga acgccaacca attcactcac acgacgaaca tagttacgcg
    10801 cattctcagg aagatcttcc aaattgcgaa ctccggtaat atcttctgac caacctggca
    10861 actcttcata gataggcttg caacgtttca attgctcaag actagctgga tagtagtcaa
    10921 tacgttgacc gtcaagatca taggccacac agattttcac agtatccaaa ccgctcaaaa
    10981 catcaataga gttcaaagaa aggttagtaa taccagaaac acgacggcta tgacgcatca
    11041 caactgagtc aaaccaacct acacgacgtg gacgaccagt tgttgtacca tactcatgac
    11101 ccacttcacg gatacgttct cccacttcat caaacaactc agttgggaaa ggaccatctc
    11161 ctacacgact cgtataagct ttacatacac ctacaacctt gtcaatcttg cttggaccga
    11221 caccagaacc aattgtcaca ccaccagcta cagggtttga tgacgtaaca aatggataag
    11281 taccttggtc gatatctagc ataacacctt gtgcaccttc aaaaagcaca cgtttgccat
    11341 tatcaagcgc atcattcaag ataacagatg tatctatcac gtatttcttg atttgttgac
    11401 catattcgta atattcttca aaaatatcat cgaaaacaat cgctttactg tcatacaatt
    11461 tttcaaaaag acgattcttt tcagcaaggt tacgttctaa acgctcacgg aaaatatctt
    11521 tatctaaaag atctgcaata cgaattccaa cacgagcagc cttgtccata taagctggac
    11581 caattccctt aattgtcgta ccaatcttat tgtcgccctt agcttcttct tgcaagcgat
    11641 ccaactcgat atgataaggc aaaataacat gcgcacgatc agaaatacgc aagttatcag
    11701 ttgttacacc ttcctcatga agatagctca actcttttac aagagattta ggatttacaa
    11761 ccataccatt cccaatgaca gatatttttt cagggaagaa aatcccagat gggatcaagt
    11821 gcaacttaaa tttcttaccg tcaatcacaa tcgtgtgacc agcattatca ccaccttggt
    11881 aacgtgcaat cacttctgca ttcgctgaaa ggaagtctgt aatcttccct ttaccttcat
    11941 caccccattg ggtacctaca acaacaactg aagtcataat cttgtctgag ccctcaggct
    12001 cttcctttct cacatacatg gcaggactct cacctgcaat tatatcttac aatttattat
    12061 aataaaaaat cgccttttta tcaagaagaa acaatagaaa gatttgctat ttccaactat
    12121 taaaaaatga tttagaaaaa ttactagcta tttactatca tctttccata aaagagtaaa
    12181 ttagttcgga aatttactaa aattacctca acaagaaata aaaccccgat tcattaccaa
    12241 tttttcaaga tacaaacgat aagcaacacg ataatggtaa acaataaaat ccttacgaca
    12301 accaatgcca tatcttacta aataataaat taaaaattta aaatgaacat gttcccagtc
    12361 aaaattatca ccaaatgtag gaccatactc ttcttcaata ctatcataga aattagccat
    12421 ctgctcataa attttttgta acataatcaa atactccttt tcttttttat aaacttattc
    12481 taacaaaaaa ttttacacat tcactatcaa ttcctgaatt gttaaaatac cagcctcaac
    12541 aagataaaaa taggaaaagt tgacaaaata gaaacaaatt tgctacctaa atttcaaaca
    12601 attaaagttc taaaaagacc gc
//
LOCUS       DBERMR010000057        12581 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.057, whole genome shotgun sequence.
ACCESSION   DBERMR010000057 DBERMR010000000
VERSION     DBERMR010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12581
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.057"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..1111
                     /gene="dnaN"
                     /locus_tag="ACL8A1_001800"
     CDS             <1..1111
                     /gene="dnaN"
                     /locus_tag="ACL8A1_001800"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HGS1202518.1"
                     /translation="LFLQALNTTKRAISSKNAIPILSTVKIDVTNEGITLIGSNGQIS
                     IENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQNQIVLTSGKS
                     EITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRPILTGVHFVL
                     SQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDDIEAVEIFFA
                     NNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSMERARLLSSAT
                     QNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYLIDSLKALNS
                     EKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            1176..1370
                     /locus_tag="ACL8A1_001801"
     CDS             1176..1370
                     /locus_tag="ACL8A1_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HGS1202519.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            1454..2569
                     /gene="ychF"
                     /locus_tag="ACL8A1_001802"
     CDS             1454..2569
                     /gene="ychF"
                     /locus_tag="ACL8A1_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HGS1202520.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARIIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            2640..3209
                     /gene="pth"
                     /locus_tag="ACL8A1_001803"
     CDS             2640..3209
                     /gene="pth"
                     /locus_tag="ACL8A1_001803"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HGS1202521.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            3210..6719
                     /gene="mfd"
                     /locus_tag="ACL8A1_001804"
     CDS             3210..6719
                     /gene="mfd"
                     /locus_tag="ACL8A1_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HGS1202522.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASG
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERDLAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTSICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKVPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNVDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LESLLIFGESLLEIKESKEENSI"
     gene            6777..7043
                     /locus_tag="ACL8A1_001805"
     CDS             6777..7043
                     /locus_tag="ACL8A1_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HGS1202523.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            7036..7404
                     /locus_tag="ACL8A1_001806"
     CDS             7036..7404
                     /locus_tag="ACL8A1_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HGS1202524.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATRLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            7409..7531
                     /locus_tag="ACL8A1_001807"
     CDS             7409..7531
                     /locus_tag="ACL8A1_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0009 family protein"
                     /protein_id="HGS1202525.1"
                     /translation="MENLLDVIEQFLSLSDEKLEELADKNQLLRLQEEKERKNA"
     gene            7524..8792
                     /locus_tag="ACL8A1_001808"
     CDS             7524..8792
                     /locus_tag="ACL8A1_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HGS1202526.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKAWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            8789..10066
                     /gene="tilS"
                     /locus_tag="ACL8A1_001809"
     CDS             8789..10066
                     /gene="tilS"
                     /locus_tag="ACL8A1_001809"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HGS1202527.1"
                     /translation="MRESDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ESIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            10070..10612
                     /gene="hpt"
                     /locus_tag="ACL8A1_001810"
     CDS             10070..10612
                     /gene="hpt"
                     /locus_tag="ACL8A1_001810"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HGS1202528.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKAREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            10628..>12581
                     /gene="ftsH"
                     /locus_tag="ACL8A1_001811"
     CDS             10628..>12581
                     /gene="ftsH"
                     /locus_tag="ACL8A1_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HGS1202529.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEETGIQFFTPSVTKVEKFTSTI
                     LSADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDE"
BASE COUNT         4325 a         1893 c         2564 g         3799 t
ORIGIN      
        1 tttatttcta caagcattaa atactactaa gagagctatt agttctaaaa atgccattcc
       61 tattttatca acagtaaaaa ttgacgtgac caatgaaggt attactttaa ttggttcaaa
      121 tggtcaaatt tcaattgaaa attttatttc tcaaaaaaat gaagatgctg gtttgttaat
      181 tacttcttta ggttcgatcc ttcttgaagc ttctttcttt atcaatgtag tatctagttt
      241 acctgatgta actcttgatt ttaaagaaat tgaacaaaat caaattgttt taaccagtgg
      301 caaatcagaa attaccctaa aaggaaaaga tagcgaacaa tatccacgaa tccaagaaat
      361 ttcagcaagc actcctttaa tacttgaaac aaaattactc aagaaaatta ttaatgaaac
      421 agcctttgct gcaagtacac aagagagtcg tccgatttta acaggtgtcc acttcgtatt
      481 gagtcaacac aaagagttaa aaacagttgc aacagactct catcgcctaa gccagaaaaa
      541 attgactctt gaaaaaaata gtgatgattt tgatgtcgta attcctagcc gttctctacg
      601 cgaattttca gcggtattta cagatgatat cgaagctgta gagattttct ttgccaataa
      661 ccaaatcctc tttagaagcg aaaatattag cttctatact cgtctcctag aaggaaacta
      721 tcctgataca gatcgcttga ttccaacaga ctttaacact actattactt ttaatgtggt
      781 aaacttacgc cagtcaatgg agcgtgcccg tcttttatca agtgcgactc aaaatggtac
      841 tgtgaaactt gaaattaagg atggggttgt tagcgcccat gttcactctc cagaagttgg
      901 taaagtaaac gaagaaatcg atactgatca ggttactggt gaagatttga ccattagttt
      961 caacccaact tacttgattg attctcttaa agctttaaat agcgaaaagg tgaccattag
     1021 ctttatctca gctgttcgtc catttactct tgtgccagca gatactgacg aagacttcat
     1081 gcagctcatt acaccagttc gtacaaatta agtgaaagag gttgagcctg gctcgcctct
     1141 tttatgatat aatcgaaaaa gaaaaggaga gtagtatgta tcaagttgga aattttgttg
     1201 agatgaaaaa atcacacgct tgtacaatca agtcgactgg taaaaaggct aatcgttggg
     1261 aaattacacg tgtaggagca gatatcaaaa taaaatgcag taattgtgag catgttgtca
     1321 tgatggggcg atatgatttt gagcgaaaaa tgaataaaat tattgactga gaacccttag
     1381 ttagagggtt agcactttat ccctttttgt gttataatat tagggattga aatgaaaacg
     1441 gagaatgaga aatatggctt tgacagcagg tatcgttggt ttgccaaacg ttggtaaatc
     1501 aacactattt aatgcaatta caaaagcagg agcagaggca gcaaactacc catttgcgac
     1561 tattgatcca aatgttggaa tggtggaagt tccagatgaa cgcctacaaa aactaactga
     1621 aatgataact cctaaaaaga cagttcccac aacatttgaa tttacagata ttgcagggat
     1681 tgtaaaagga gcttcaaaag gagaagggct agggaataaa ttcttggcca atattcgtga
     1741 agtagatgcg attgttcacg tagttcgtgc ttttgatgat gaaaatgtaa tgcgcgagca
     1801 aggacgtgaa gacgcctttg tagatccact tgcagatatt gatacaatta atctggaatt
     1861 aattcttgct gacttagaat cagtgaacaa acgatatgcg cgtgtagaaa agatggcacg
     1921 tacgcaaaaa gataaagaat cagtagcaga attcaatgtt cttcaaaaga ttaaaccagt
     1981 cctagaagac gggaaatcag ctcgtatcat tgaatttaca gatgaggaac aaaaggttgt
     2041 caaaggtctt ttccttttga cgactaaacc agttctttat gtagctaatg tggacgagga
     2101 tgtggtttca gaacctgact ctatcgacta tgtcaaacaa attcgtgaat ttgcagcgac
     2161 agaaaatgct gaagtagtcg ttatttctgc gcgtgctgag gaagaaattt ctgaattgga
     2221 tgatgaagat aaaaaagagt ttcttgaagc cattggtttg acagaatcag gtgtagataa
     2281 gttgacgcgt gcagcttacc acttgcttgg attgggaact tacttcacag ctggtgaaaa
     2341 agaagttcgc gcttggactt tcaaacgtgg tatgaaggct cctcaagcag ctggtattat
     2401 ccactcagac tttgaaaaag gctttattcg tgcagtaacc atgtcatatg aagatctagt
     2461 gaaatacgga tctgaaaagg ccgtaaaaga agctggacgc ttgcgtgaag aaggaaaaga
     2521 atatatcgtt caagatggcg atatcatgga attccgcttt aatgtctaaa aattaataaa
     2581 tggtgtcaat taggttggaa aaaaattcca acccttttgg cttttgaaag gaaaaataaa
     2641 tgaccaaatt acttgtaggc ttgggaaatc caggggataa atattttgaa acaaaacaca
     2701 atgttggttt tatgttgatt gatcaactag cgaagaaaca gaatgtcact tttacacacg
     2761 ataagatatt tcaagctgac ctagcatcct ttttcctaaa tggagaaaaa atttatctgg
     2821 ttaaaccaac gacctttatg aatgaaagtg gaaaagcagt tcatgcttta ttaacttact
     2881 atggtttgga tattgacgat ttacttatca tttacgatga tcttgacatg gaagttggga
     2941 aaattcgttt aagagcaaaa ggctcagcag gtggtcataa tggtatcaag tctattattc
     3001 aacatatagg aactcaggtc tttaaccgtg ttaagattgg aattggaaga cctaaaaatg
     3061 gtatgtcagt tgttcatcat gttttgagta agtttgacag ggatgattat atcggtattt
     3121 tacagtctgt tgacaaagtt gacgattctg taaactacta tttacaagag aaaaattttg
     3181 agaaaacaat gcagaggtat aacggataaa tggtgacctt attagattta ttctcagaaa
     3241 atgatcagat taaaaaatgg catcaaaatt taacagataa gaaaagacaa ctaatacttg
     3301 gtttatcaac atctactaag gcgcttgcaa ttgcaagcgg tttagaaaaa gaagatagga
     3361 ttgtgttatt gacgtcaact tatggagaag cagaaggact tgttagtgat cttatatcta
     3421 tcttgggtga ggaactcgtc tatccatttt tggtagatga tgctcctatg gtggagtttt
     3481 tgatgtcttc acaggaaaaa attatttcac gggttgaagc cttgcgtttt ttgactgatt
     3541 catctaagaa agggatttta gtttgtaata tcgcagcaag tcgattgatt ttaccgtctc
     3601 ccaatgcatt caaagatagt attgtaaaaa tctcagttgg tgaagaatat gatcaacacg
     3661 cgtttatcca tcagttaaag gaaaatggct atcgaaaagt tactcaagta caaactcagg
     3721 gcgaatttag tcttcgagga gatattttag atatttttga aatatcccag ttagaacctt
     3781 gtcgaattga gttttttggt gatgaaattg atggtatcag gtcatttgaa gtagaaacac
     3841 aattatcgaa agaaaataag acagaactca ctatctttcc agctagtgat atgcttttga
     3901 gagaaaagga ttatcaacga ggacagtcag ctttagaaaa acaaatttca aaaactttat
     3961 cacctatttt gaaatcatac ctagaagaaa ttctttcaag ttttcatcaa aagcaaagtc
     4021 atgcagattc tcggaagttt ttatctttgt gctatgataa gacatggact gtctttgatt
     4081 atattgaaaa agatactcca atattctttg atgattatca aaaattgatg aatcagtatg
     4141 aagtatttga aagagactta gcgcagtact ttacagaaga attacagaat agtaaagcat
     4201 tttctgatat gcagtatttt tctgatattg aacaaatcta taaaaaacaa agtccagtga
     4261 cctttttctc taatcttcaa aagggtttag gaaatctcaa atttgacaaa atttatcaat
     4321 tcaatcaata tcctatgcag gaatttttca atcagttttc ttttctaaaa gaagaaattg
     4381 aacgatataa aaaaatggat tacaccatta ttctgcagtc tagcaattca atgggaagta
     4441 aaacattgga ggatatgtta gaggaatatc agattaaatt ggattctaga gataagacaa
     4501 gtatctgtaa agaatctgta aacttaatag agggtaatct cagacatggt tttcattttg
     4561 tagatgaaaa gattttattg ataactgaac atgagatttt tcaaaagaaa ttaaagcgtc
     4621 gttttcgaag acaacatgtt tcaaatgcag agagattaaa agattacaat gaacttgaaa
     4681 aaggggacta tgttgtccat catatccatg ggattggtca atatctagga attgaaacca
     4741 ttgaaatcaa gggaattcat cgcgattatg tcagtgtcca ataccaaaat ggtgatcaaa
     4801 tttctattcc cgtggaacag attcatctac tgtccaaata tatttcaagt gatggtaaag
     4861 ttccaaaact caataaatta aatgatggtc attttaaaaa ggccaagcaa aaggttaaga
     4921 accaggtaga ggatatagct gatgatttaa tcaaactcta ctctgaacgt agtcagttga
     4981 agggttttgc tttctcagct gatgatgatg atcaagatgc ctttgatgat gctttccctt
     5041 atgttgaaac ggatgatcaa cttcgtagta ttgaggaaat caagagggat atgcaggctt
     5101 ctcagccaat ggatcgactt ttagttgggg atgttggttt tggaaagact gaagttgcta
     5161 tgcgtgcagc ctttaaagca gtcaatgatc acaaacaggt tgtcattcta gttccgacga
     5221 cggttttagc gcaacagcac tatacgaatt ttaaggaacg attccaaaat tttgcagtta
     5281 atgttgatgt gttgagtcgc tttagaagta aaaaagagca gactgcaaca cttgaaaaat
     5341 tgaaaaacgg tcaagtcgat attttgattg gaacacatcg tgttttgtca aaagatgttg
     5401 tgtttgctga tttgggcttg atgattattg atgaggaaca gcgatttggt gtcaagcata
     5461 aggaaacttt gaaagaactg aagaaacaag tggatgtcct aaccttgacc gctacgccaa
     5521 tccctcgtac cctccatatg tctatgctgg gaatcagaga tttgtctgtt attgaaactc
     5581 cgccgactaa tcgctatcct gttcagacct atgttttgga aaagaatgat agtgtcattc
     5641 gtgatgctgt cttgcgtgaa atggagcgtg gaggtcaagt ttactatctt tacaacaaag
     5701 ttgacacaat tgttcagaag gtttcagaat tacaggagtt gattccggag gcttcgattg
     5761 gatatgttca tggtcgaatg agtgaagtcc agttggaaaa tactctatta gactttattg
     5821 agggacaata cgatatcttg gtgacgacta ctattattga gacaggggtg gacattccaa
     5881 atgctaatac tttatttatt gaaaatgcgg accatatggg cttgtcaacc ttatatcagt
     5941 taagaggaag agtcggtcgt agtaatcgta ttgcttatgc ttatctcatg tatcgtccag
     6001 aaaaatcaat cagtgaagtc tctgaaaaga gattagaagc gattaaagga tttacagaat
     6061 tgggctctgg ctttaagatt gcaatgcgag atctttcgat tcgtggagca ggaaatcttt
     6121 taggaaaatc ccagtctggt ttcattgatt ctgttggttt tgaattgtat tcgcagttat
     6181 tagaggaagc tattgctaaa cgaaacggta atgctaacgc taacacaaga accaaaggga
     6241 atgctgagtt gattttgcaa attgatgcct atcttcctga tacttatatt tctgatcaac
     6301 gacataagat tgaaatttac aagaaaattc gtcaaattga caaccgtgtc aattatgaag
     6361 agttacaaga ggagttgata gaccgttttg gagaataccc agatgtagta gcctatcttt
     6421 tagagattgg tttggttaaa tcatacttgg acaaggtctt tgttcaacgt gtggaaagaa
     6481 aagataataa aattacaatt caatttgaaa aagtcactca acgactgttt ttagctcaag
     6541 attattttaa agctttatcc gtaacgaact taaaagcagg catcgctgag aataagggat
     6601 taatggagct tgtatttgat gtccaaaata agaaagatta tgaaatttta gaaagtttgc
     6661 tgatttttgg agaaagttta ttagaaataa aagagtctaa ggaagaaaat tccatttgat
     6721 atttttcttc tataaaatag ataaaaatgg tacaataata aattgaggta ataaggatga
     6781 gattagataa atatttaaaa gtatcgcgaa ttatcaagcg tcgtacagtc gcaaaggaag
     6841 tagcagataa aggtagaatc aaggttaatg gaatcttggc caaaagttca acggacttga
     6901 aagttaatga ccaagttgaa attcgctttg gcaataagtt gctacttgta aaagtactag
     6961 agatgaaaga tagtacaaaa aaagaagatg cagcaggcat gtatgaaatt atcagtgaaa
     7021 cacgggtaga agaaaatgtc taaaaatatt gtacaattga ataattcttt tattcaaaat
     7081 gaataccaac gtcgtcgcta cctgatgaaa gaacgacaaa aacggaatcg ttttatgggg
     7141 ggggtattga ttttgattat gctattattt atcttgccaa cttttaattt agcgcagagt
     7201 tatcagcaat tactccaaag acgtcagcaa ttagcagact tgcaaactca gtatcaaact
     7261 ttgagtgatg aaaaggataa ggagacagca tttgctacca ggttgaaaga tgaagattat
     7321 gctgctaaat atacacgagc gaagtactat tattctaagt cgagggaaaa agtttatacg
     7381 attcctgact tgcttcaaag gtgataaaat ggaaaattta ttagacgtaa tagagcaatt
     7441 tttgagtttg tcagatgaaa agctggaaga attggctgat aaaaatcaat tattgcgttt
     7501 acaagaagaa aaggaaagga agaatgcgta aattcttaat tattttgttg ctaccaagtt
     7561 ttttgaccat ttcaaaagtc gttagcacag aaaaagaagt cgtctatact tcgaaagaaa
     7621 tttattacct ttcacaatct gactttggta tttattttag agaaaaatta agttctccca
     7681 tggtttatgg agaggttcct gtttatgcga atgaagattt agtagtggaa tctgggaaat
     7741 tgactcccaa aacaagtttt caaataaccg agtggcgctt aaataaacaa ggaattccag
     7801 tatttaagct atcaaatcat caatttatag ctgcggacaa acgattttta tatgatcaat
     7861 cagaggtaac tccaacaata aaaaaagcat ggttagaatc tgactttaaa ctgtacaata
     7921 gtccttatga tttaaaagaa gtgaaatcat ccttatcagc ttattcgcaa gtatcaatcg
     7981 acaagaccat gtttgtagaa ggaagagaat ttctacatat tgatcaggct ggatgggtag
     8041 ctaaagaatc aacttctgaa gaagataatc ggatgagtaa agttcaagaa atgttatctg
     8101 aaaaatatca gaaagattct ttctctattt atgttaagca actgactact ggaaaagaag
     8161 ctggtatcaa tcaagatgaa aagatgtatg cagccagcgt tttgaaactc tcctatctct
     8221 attatacgca agaaaaaata aatgagggtc tttatcagtt agatacgact gtaaaatacg
     8281 tatctgcagt caatgatttt ccaggttctt ataaaccaga gggaagtggt agtcttccta
     8341 aaaaagaaga taataaagaa tattctttaa aggatttaat tacgaaagta tcaaaagaat
     8401 ctgataatgt agctcataat ctattgggat attacatttc aaaccaatct gatgccacat
     8461 tcaaatccaa aatgtctgcc attatgggag atgattggga tccaaaagaa aaattgattt
     8521 cttctaagat ggccgggaag tttatggaag ctatttataa tcaaaatgga tttgtgctag
     8581 agtctttgac taaaacagat tttgatagtc agcgaattgc caaaggtgtt tctgttaaag
     8641 tagctcataa aattggagat gcggatgaat ttaagcatga tacgggtgtt gtctatgcag
     8701 attctccatt tattctttct attttcacta agaattctga ttatgatacg atttctaaga
     8761 tagccaagga tgtttatgag gttctaaaat gagggaatca gattttttaa atcattttct
     8821 caagaaggga tatttcaaaa agcatgctaa ggcggttcta gctctttctg gtggattaga
     8881 ttctatgttt ctatttaagg tattgtctac ttatcaaaaa gagttagaga ttgaattgat
     8941 tctagctcat gtgaatcata agcagagaat tgaatcagat tgggaagaaa aggaattaag
     9001 gaagttggct gctgaagcag agcttcctat ttatatcagc aatttttcag gagaattttc
     9061 agaagcgcgt gcacgaaatt ttcgttatga tttttttcaa gaggtcatga aaaagacagg
     9121 tgcgacagct ttagtcactg cccaccatgc tgatgatcag gtggaaacga ttttgatgcg
     9181 cttgattcga ggaactcgct tgcgctatct atcaggaatt aaggagaagc aagtagtcgg
     9241 agagatagaa atcattcgtc ccttcttgca ttttcagaaa aaagactttc catcaatttt
     9301 tcactttgaa gatacatcaa atcaggagaa tcattatttt cgaaatcgta ttcgaaactc
     9361 ttacttacca gaattggaaa aagaaaatcc tcgatttagg gatgcaatct taggcattgg
     9421 caatgaaatt ttagattatg atttggcaat agctgaatta tctaacaata ttaatgtgga
     9481 agatttacag cagttatttt cttactctga gtctacacaa agagttttac ttcaaactta
     9541 tctgaatcgt tttccagatt tgaatcttac aaaagctcag tttgctgaag ttcagcagat
     9601 tttaaaatct aaaagccagt atcgtcatcc gattaaaaat ggctatgaat tgataaaaga
     9661 gtaccaacag tttcagattt gtaaaatcag tccgcaggct gatgaaaagg aagatgaact
     9721 tgtgttacac tatcaaaatc aggtagctta tcaaggatat ttattttctt ttggacttcc
     9781 attagaaggt gaatcaattc aacaaatacc tgtttcacgt gaaacatcca tacacattcg
     9841 tcatcgaaaa acaggagatg ttttgattca aaatgggcat agaaaaaaac tcagacgttt
     9901 atttattgat ttgaaaatcc ctatggaaaa gagaaactct gctcttatta ttgagcaatt
     9961 tggtgaaatt gtctcaattt tgggaattgc gaccaataat ttgagtaaaa aaacgaaaaa
    10021 tgatataatg aacactgtac tttatataga aaaaatagat aggtaaaaaa tgttagaaaa
    10081 cgatattaaa aaagtcctcg tttcacacga tgaaattaca gaagcagcta aaaaactagg
    10141 tgctcaatta actaaagatt atgcaggaaa aaatccaatc ttagttggga ttttaaaagg
    10201 atctattcct tttatggctg aattggtcaa acatattgat acacatattg aaatggactt
    10261 catgatggtt tctagctacc atggtggaac agcaagtagt ggtgttatca atattaaaca
    10321 agatgtgact caagatatca aaggaagaca tgttctattt gtagaagata tcattgatac
    10381 aggtcaaact ttgaagaatt tgcgagatat gtttaaagca agagaagcag cttctgttaa
    10441 aattgcaacc ttgttggata aaccagaagg acgtgttgta gaaattgagg cagactatac
    10501 ctgctttact atcccaaatg agtttgtagt aggttatggt ttagactaca aagaaaatta
    10561 tcgtaatctt ccttatattg gagtattgaa agaggaagtg tattcaaatt agaaagaata
    10621 atctttaatg aaaaaacaaa ataatggttt aattaaaaat ccttttctat ggttattatt
    10681 tatctttttc cttgtgacag gattccagta tttctattct gggaataact caggaggaag
    10741 tcagcaaatc aactatactg agttggtaca agaaattacc gatggtaatg taaaagaatt
    10801 aacttaccaa ccaaatggta gtgttatcga agtttctggt gtctataaaa atcctaaaac
    10861 aagtaaagaa gaaacaggta ttcagttttt cacgccatct gttactaagg tagagaaatt
    10921 taccagcact attctttctg cagatactac cgtatcagaa ttgcaaaaac ttgctactga
    10981 ccataaagca gaagtaactg ttaagcatga aagttcaagt ggtatatgga ttaatctact
    11041 cgtatccatt gtgccatttg gaattctatt cttcttccta ttctctatga tgggaaatat
    11101 gggaggaggc aatggccgta atccaatgag ttttggacgt agtaaggcta aagcagcaaa
    11161 taaagaagat attaaagtaa gattttcaga tgttgctgga gctgaggaag aaaaacaaga
    11221 actagttgaa gttgttgagt tcttaaaaga tccaaaacga ttcacaaaac ttggagcccg
    11281 tattccagca ggtgttcttt tggagggacc tccggggaca ggtaaaactt tgcttgctaa
    11341 ggcagtcgct ggagaagcag gtgttccatt ctttagtatc tcaggttctg actttgtaga
    11401 aatgtttgtc ggagttggag ctagtcgtgt tcgctctctt tttgaggatg ccaaaaaagc
    11461 agcaccagct atcatcttta tcgatgaaat tgatgctgtt ggacgtcaac gtggagtcgg
    11521 tctcggcgga ggtaatgacg aacgtgaaca aaccttgaac caacttttga ttgagatgga
    11581 tggttttgag ggaaatgaag ggattatcgt catcgctgcg acaaaccgtt cagatgtact
    11641 tgaccctgcc cttttgcgtc caggacgttt tgatagaaaa gtattggttg gtcgtcctga
    11701 tgttaaaggt cgtgaagcaa tcttgaaagt tcacgctaag aataagcctt tagcagaaga
    11761 tgttgatttg aaattagtgg ctcaacaaac tccaggcttt gttggtgctg atttagagaa
    11821 tgtcttgaat gaagcagctt tagttgctgc tcgtcgcaat aaatcgataa ttgatgcttc
    11881 agatattgat gaagcagaag atagagttat tgctggacct tctaagaaag ataagacagt
    11941 ttcacaaaaa gaacgagaat tggttgctta ccatgaggca ggacatacca ttgttggtct
    12001 agtcttgtcg aatgctcgcg ttgtccataa ggttacaatt gtaccacgcg gccgtgcagg
    12061 cggatacatg attgcacttc ctaaagagga tcaaatgctt ctatctaaag aagatatgaa
    12121 agagcaattg gctggcttaa tgggtggacg tgtagctgaa gaaattatct ttaatgtcca
    12181 aaccacagga gcttcaaacg actttgaaca agcgacacaa atggcacgtg caatggttac
    12241 agagtacggt atgagtgaaa aacttggccc agtacaatat gaaggaaacc atgctatgct
    12301 tggtgcacag agtcctcaaa aatcaatttc agaacaaaca gcttatgaaa ttgatgaaga
    12361 ggttcgttca ttattaaatg aggcacgaaa taaagctgct gaaattattc agtcaaatcg
    12421 tgaaactcac aagttaattg cagaagcatt attgaaatac gaaacattgg atagtacaca
    12481 aattaaagct ctttacgaaa caggaaagat gcctgaagca gtagaagagg aatctcatgc
    12541 actatcctat gatgaagtaa agtcaaaaat gaatgacgaa a
//
LOCUS       DBERMR010000058        12440 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.058, whole genome shotgun sequence.
ACCESSION   DBERMR010000058 DBERMR010000000
VERSION     DBERMR010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12440
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.058"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            436..1146
                     /gene="deoD"
                     /locus_tag="ACL8A1_001812"
     CDS             436..1146
                     /gene="deoD"
                     /locus_tag="ACL8A1_001812"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HGS1202530.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLGDAVCFNEVRN
                     MFGYTGTYKGHRVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKELGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(1169..1693)
                     /locus_tag="ACL8A1_001813"
     CDS             complement(1169..1693)
                     /locus_tag="ACL8A1_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HGS1202531.1"
                     /translation="MKITIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFLPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(1746..1982)
                     /gene="rpsT"
                     /locus_tag="ACL8A1_001814"
     CDS             complement(1746..1982)
                     /gene="rpsT"
                     /locus_tag="ACL8A1_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HGS1202532.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(2050..2970)
                     /gene="coaA"
                     /locus_tag="ACL8A1_001815"
     CDS             complement(2050..2970)
                     /gene="coaA"
                     /locus_tag="ACL8A1_001815"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HGS1202533.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEEKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVEAFAHQVWTSINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            3160..3527
                     /locus_tag="ACL8A1_001816"
                     /pseudo
     CDS             3160..3527
                     /locus_tag="ACL8A1_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3734..4324
                     /locus_tag="ACL8A1_001817"
     CDS             3734..4324
                     /locus_tag="ACL8A1_001817"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGS1202534.1"
                     /translation="MSKMYYAENPDAAHDIHELRVELLGQKMTFLTDAGVFSKKIVDF
                     GSQLLLKCLEVNQGETVLDVGCGYGPLGLSLAKAYGVQATMVDINTRALDLARRNAEK
                     NNAKATIFQSNIYEQVEGHFDHVISNPPIRAGKQVVHEIIEKSKDFLETGGDLTIVIQ
                     KKQGAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            4321..5598
                     /locus_tag="ACL8A1_001818"
     CDS             4321..5598
                     /locus_tag="ACL8A1_001818"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HGS1202535.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLVEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGKGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            5616..6278
                     /gene="deoC"
                     /locus_tag="ACL8A1_001819"
     CDS             5616..6278
                     /gene="deoC"
                     /locus_tag="ACL8A1_001819"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HGS1202536.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKIVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            6265..6654
                     /locus_tag="ACL8A1_001820"
     CDS             6265..6654
                     /locus_tag="ACL8A1_001820"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HGS1202537.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGNVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMVEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            6745..7797
                     /locus_tag="ACL8A1_001821"
     CDS             6745..7797
                     /locus_tag="ACL8A1_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HGS1202538.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQDWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            7941..9476
                     /locus_tag="ACL8A1_001822"
     CDS             7941..9476
                     /locus_tag="ACL8A1_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1202539.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGISGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            9469..10527
                     /locus_tag="ACL8A1_001823"
     CDS             9469..10527
                     /locus_tag="ACL8A1_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1202540.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIMIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            10530..11486
                     /locus_tag="ACL8A1_001824"
     CDS             10530..11486
                     /locus_tag="ACL8A1_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1202541.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPAIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGIIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
     gene            11571..12440
                     /locus_tag="ACL8A1_001825"
                     /pseudo
     CDS             11571..12440
                     /locus_tag="ACL8A1_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         3647 a         2196 c         2820 g         3777 t
ORIGIN      
        1 ggtttgaaga gattttcgaa gagtataaac agaaaggtag agcgcgtgtt ctaatttgaa
       61 cacgagtaga aaacttttct aaaaacaaaa acgaaaggat gggtaaactg tattcgctga
      121 actgaatacg ggcgactctc ctctaaatca aaattaagaa agaaaggaat tgaacccacc
      181 ctaaaagcag tgggaaaaag atagttggtc tagcgagcat cgctcactgc gcccaactcc
      241 tattttccct tcgctttttg acgggtttgg tatctttctc aatataaaat ataaaataaa
      301 gaaaggtaga gcgtgtgttt tgatttgaac acgagcggaa aactcggaaa atagataatc
      361 tgactgaaaa atcaggattt ctcgtcggct tcctaatttt cagtcgtttt cttctcgctc
      421 tttgtatcat aaattatgtc tatccatatt gctgctcagc agggtgaaat tgctgataaa
      481 attcttcttc ctggggatcc tcttcgtgct aagtttattg cggagaattt ccttggtgat
      541 gctgtttgtt ttaacgaagt gcgtaacatg tttggttaca ctggtactta caagggtcac
      601 cgtgtatctg tcatgggaac tgggatggga atgccatcta tttcgattta tgcgcgtgag
      661 ttaatcgtag actacggtgt gaagaaattg attcgtgtgg gaactgcagg ttctttgaat
      721 gaagaggttc atgttcgtga attagttttg gcgcaggcgg ctgcaaccaa ctcaaacatc
      781 gttcgtaatg actggccaca gtacgatttt ccacaaattg ctagctttga tttgcttgat
      841 aaagcctacc atatcgccaa agaacttggt atgactactc acgttgggaa cgttttgtca
      901 tctgatgtct tttactcaaa ttactttgaa aagaatatcg agcttggtaa atggggagtc
      961 aaggctgtgg aaatggaagc agcagctctt tactatcttg ctgcccaata ccatgttgat
     1021 gcgctagcta tcatgaccat ctctgatagc ttggtcaatc cagacgaaga cacaactgca
     1081 gaagaacgtc aaaatacctt cactgatatg atgaaggttg gtttagaaac cttgattgca
     1141 gaataattat agccaaaaag gaagcccttt atgaattgta ggacttcctt ttcttatcca
     1201 gaaattgatc tagctctctc tgatttcgaa ggatagtgac tttatgtgaa tattcttggc
     1261 aaagtttttg gtaattttct ttttgagttt tgctacgccc atcccaaaga atccatctga
     1321 taaactccca ctcaaagcgt tcagggcaat ctgccgccat actttctctg acttttccac
     1381 ggtatttaag ataacgctta aaggctctaa agagacaggt caatggcaaa aaattgagaa
     1441 agatgatttg gtcagcttct tgcattcgtt cttggtagta gcaccaagaa taattaccat
     1501 cgatgaccca agctttatgc ttggtgagaa agttttttat ctcggttaac atccattcgc
     1561 agtcactgtc ttgccaacca ggttgaaatt ggagtgtgtc catgtgcagt tttggaatgg
     1621 agtagtagtt agataacttt tctgctagag ttgacttacc agaaccagaa tatccgataa
     1681 ttgtgatttt cattttctac cttttcctat ttggagacaa aaaaacagcc tctatggact
     1741 gtttcttatt tagcaagttt agctgaaaga cgagctttat cgcggcttgc tttgtttttg
     1801 tgaatcaaac ctttagtttc tgctttatcg atagctgagc tagcagcacg gaaaagttct
     1861 tcagatgggt ttgcttcgaa agcttttata gcagtacgca tagctgattt ttgagctgag
     1921 ttcttttcgt tttgtttaac gttcaattca gcgcgtttga tagctgattt aatgtttgcc
     1981 aatgttctta cctccatatt tactaactat accattatat atgaaaactt acgttttgac
     2041 aagggaaaat tattttttta agtaaatttc atcgatttca tggttcttgc ttttatgaag
     2101 aatcacttct gcacgatttc tggttggttc aatataattt tgtagatttg tgagattgat
     2161 actggtccag acctgatggg caaaggcttc cacttcccca atcggcatct gagtaaaacg
     2221 ataatagtag ctatcagggt cgttttgggc tagactcagc attttcaaga aacggtccag
     2281 ataccaactt tcaatatcat ccactccagc atctacatag atggaaaagt caaagaagtc
     2341 agtgatatag agacgatcgt tttgtggatt ttgaaagaca ttgattccct caacaattac
     2401 aaaatcagca gctttgacac tttgtttctc ttcgggtacg atgtcgtaaa cttcatgaga
     2461 atagacagga atatctacat cttgtccatt tttgatgcgg tccaagaagt tgagaagagc
     2521 ttccatatca tagctttcag gaaatccttt acgatttaaa atcccctgct caatcaaggt
     2581 ttgattggga tagagaaaac catcagttgt aaccaactca accgtagcat ctgtaaacgt
     2641 acgggacagt aggatttgaa gtaggcgact ggttgtggat tttccaacgg caacactccc
     2701 agaaacccca ataataaaag gttgagattt actttcacgt tggaggaaaa ttccttttga
     2761 aaaggctaaa tcttccttag ttcgcttgta aatctgaatc agatgagcca aggggagata
     2821 gatatctgta acgtcttgga gactgatttg gtcattaaaa ctcttgatag attccaattc
     2881 ttcttctgtc aaaggaggtg ttgtctttcg atgtaaagat tgccaagtct ggcggctgat
     2941 tttttcaaaa tgtaaaaatt cgttggtcat ttgttttccc ctaggtattt tatctatcat
     3001 accataaaaa gctaaaaaaa taaagcggtt tcttgaatgt tgtaagtcaa tgatatataa
     3061 tataagcgat caatttattg atgaaatagg agatagattc tactgattta taagcttata
     3121 aagggggctt tttagttcac aacttatagg aggtgtacta tgaaaatctt aaaacgttac
     3181 atattggaac tctgttttat tttaagtttt gctttacctt ttataaaagg agccaatgca
     3241 gataatggta gatgctttgt ggaaacctat tacggtttta cttttttgat ggaacatgct
     3301 attgtaacag ctgtctttat ctgttcgttc ttaattgctg tcttactaaa aaacgatggg
     3361 cgaaatggat tgcgacagag ggttatttct tccgcatgtc actagaagat ttgatacgac
     3421 tttggacaag tttggaaatc ctgacacaaa cgtatcagtt gggcttttat ctaaacattt
     3481 tgttgggaat tttgttgata ataaagtatt ttaaggttaa gcaatagaaa taataatctg
     3541 cctgattata gatgataaac tttttgtttt tatctggact gtgatattta tagttctaaa
     3601 tggtttatta tttataagtg ttgaaagaga ttgaagaaaa tggcttcgtg actcgctttc
     3661 ttcggtcttt ttcgttttat tatcaatttg taaacgattt acaatttgag tcaaattatg
     3721 gtaaaatagt gtcatgagta aaatgtatta tgcagaaaat cctgacgctg ctcacgacat
     3781 tcatgagttg agagtagagt tgttgggaca aaaaatgacc tttttaacgg atgcgggtgt
     3841 ttttagcaag aaaatagttg actttggaag tcaactattg ctcaagtgtc ttgaggtcaa
     3901 ccaaggagaa actgtccttg atgtaggctg tggttacgga cctttgggtt tgtccttagc
     3961 taaggcttat ggagttcagg ctactatggt tgatatcaac actcgtgcct tagatttagc
     4021 tcggagaaat gctgaaaaaa ataatgcaaa agcgacgatt ttccagtcca acatctatga
     4081 acaagttgaa ggacattttg atcatgtcat ttccaatcct cctatccgtg cgggcaagca
     4141 agtcgttcat gaaatcattg agaaaagtaa agatttcttg gaaactggtg gggatttgac
     4201 aatcgtcatc cagaaaaaac aaggggctcc aagtgctaag tccaagatgg aggatgtgtt
     4261 tggtaattgt gaaatcctta aaaaagacaa gggatactat atccttagaa gtgtgaaaga
     4321 atgagagcag ttgatttaat acaaaaaaaa cgagacggtc aagaactgac ttcaagtgaa
     4381 attgaatggc tggtagaagg ctatgtgtca ggaactgttc ctgattatca gatgtctgcc
     4441 tttgctatgg ctgtttattt taaaggaatg acgactcgtg aaatttctga tttaacgatg
     4501 aatatggtta agactgggca agagtttgac ctgtcagcca ttgatggtgt gaaagttgac
     4561 aagcattcta ctggtggtgt cggcgataag gtaaccttga ttctagctcc tcttgttgct
     4621 agcttcggtg tgcctgtagc aaaaatgagt ggtcgtggtc ttggccatac aggcggaaca
     4681 attgataaat tggagtccat taagggctat caagtagaac gtagtcaaga ggattttatt
     4741 cgtcaggttc aggatattgg agtatctgtc attgggcaat cagaccagct ggtcaaagca
     4801 gataagcttc tctatgccct tcgtgatgtg accgcaactg tcgacacgat tcctttgatt
     4861 gcgagttcgg tgatgagcaa gaaaattgcg gcaggggcgg atgctatttt gctagacgtg
     4921 actgtcggtg agggtgcctt catgaagacg gttgatgagg cgcgtgagtt ggctcaaact
     4981 atggtagatc ttggtaaggt tgttggacgg aagacagtag cagtgattac ggatatgagc
     5041 cagcctttgg gacgagcgat tggaaatcgt ctggaaatcc ttgaagcatt ggagattttg
     5101 caaggtaaag gacgtcagga tattacccac tttatttgtg aattggctca aattatgctt
     5161 ggtttggcaa atgttaacaa gactgttgaa gaagttcgcc aacatcttga gaatggtcaa
     5221 gcactggcta agtttgagga aatggtgcaa gcccaaggtg gagacttgga agacctctat
     5281 cgtcctgtaa atgtagctca tgtggtggaa atccctgctc aggaaacggg tgtcatttca
     5341 gctcttccag ctatggactt tggtctttat gccatgaggc tgggtgctgg tcgtgcagtt
     5401 aagtctgatg ccttggacta tgaaacagga attgtttttg aaaagaagat tggagactcc
     5461 gttcaaaagg gtgaaattgt tgcaaaagta tacacaaatg aaaaaattcc acctcaacta
     5521 gttacagatt tccaaaaatg tgttaaaata agtgatgaag tgaaaaaaat ccgtgaaatt
     5581 gtagaaatca tctcctaaat ggagggaaaa ccgaaatgaa attaaataaa tatatagatc
     5641 atacgctttt aaaacaagat gcaaagaaaa aacaaattga tagtttgttg tctgaggcta
     5701 gagagtatga ctttgccagt gtttgcgtta atccgacctg ggttgaacat gctaaaaaag
     5761 gacttgaagg cacagatgtt aaggtttgca cagtagtagg tttccctttg ggagcaacaa
     5821 cttcagccgt gaaagcattt gagacaaaag aagctatcca aaatggtgca gatgagattg
     5881 atatggtgat caatgttgga gctctcaaat caggtaattt agccttggtt gagtcagata
     5941 ttcgcgcagt agtggaagca agtggtgata agttagtgaa agtcattatt gaagcttgcc
     6001 ttctgacaga ccaagaaaaa attgttgttt gccaattggc ccaaaaagct ggggctgact
     6061 ttgttaaaac atctactggt ttttcaactg gtggtgctac gatagcagat gttacattaa
     6121 tgcgtgaaac agttggatct gatatgggag tcaaggctgc tggtggagct cgttcttatg
     6181 cagatgctct tgcctttgtc gaagcaggtg cgacccgtat cggaacgtca gctggggtag
     6241 ctattttaaa aggagaattg gcagatggcg actactgagt tgattgaact ggcaattgaa
     6301 accagcaaac atgcctatgt cccctattct cactttccta tcggagcagt tttagtagcc
     6361 aaagacggaa atgtttacac gggagtgaat atcgaaaatg ctagctatcc tttgaccaat
     6421 tgcggtgaga gaacagctat ttttaaggct atatctgaag gccaaagaga attttcagaa
     6481 ttgattgtct atggtcaaac tgaaaaaccg atttcaccat gtggtgcttg tcgccaagta
     6541 atggtcgaat tttttgaaca agatctaaaa gtgaccttag tcgcaaaaga taaatcgacg
     6601 gtcgagatga cggtcgggga gttacttcca tattctttta cagacttaaa ttagtctgag
     6661 tcgctctttg agtggcaagg gtctttgtga ccaatcaatc catacttgca acatcgttgc
     6721 acatcttatt taggaggttc agtaatgaac aagaaacaat ggctaggtct tggcctagtt
     6781 gcagtggcag cagttggact tgctgcatgt ggtaaccgct cttctcgtaa cgcagcttca
     6841 tcttctgatg tgaagacaaa agcagcaatc gtcactgata ctggtggtgt tgatgacaaa
     6901 tcattcaacc aatcagcttg ggaaggtttg caagactggg gtaaagaaca caatctttca
     6961 aaagataacg gtttcactta cttccaatca acaagtgaag ctgactacgc taacaacttg
     7021 caacaagcgg ctggaagtta caacctaatc tttggtgttg gttttgccct tcacaatgca
     7081 gttgaagaag cagctaaaga acacactgac ttgaactatg tcttgattga tgatgtgatt
     7141 aaagaccaaa agaatgttgc gagcgtaact ttcgctgata atgagtcagg ttaccttgca
     7201 ggtgtggctg cagcaaaaac aactaagaca aaacaagttg gttttgtagg tggtatcgaa
     7261 tctgaagtta tctctcgttt tgaagcagga ttcaaggctg gtgttgcgtc agtagaccca
     7321 tctatcaaag tccaagttga ctacgctggt tcatttggtg atgcggctaa aggtaaaaca
     7381 attgcagccg cacaatacgc agccggtgca gatattgttt accaagtagc tggtggtaca
     7441 ggtgcaggtg tctttgcaga ggcaaaatct ctcaacgaaa gccgtcctga aaatgaaaaa
     7501 gtttgggtta tcggtgttga ccgtgaccaa gaagcagaag gtaaatacac ttctaaagat
     7561 ggcaaagaat caaactttgt tcttgtatct actttgaaac aagttggtac aactgtaaaa
     7621 gatatttcta acaaggcaga aaaaggagaa ttccctggcg gtcaagtgat cgtttactca
     7681 ttgaaggata aaggggttga cttggcagta acaaaccttt cagaagaagg taaaaaagct
     7741 gtcgaagatg caaaagctaa aatccttgat ggaagcgtaa aagttcctga aaaataatgg
     7801 atggaagtca tttcttagga agcggccaag ggccgctttt ttaagagttg agacaaagtc
     7861 attttgtctc agtaaagctt gctactagac aaactagcaa gttttattga aataaaataa
     7921 gactctgaaa ggaagagcac atggcacacg aaaatgtcat tgagatgcgt gatattacca
     7981 aggtgtttgg tggatttgtt gctaacgaca aaatcaactt gcacctacga aaaggtgaaa
     8041 ttcatgcact tttaggagaa aatggggctg gtaagtccac gctaatgaac atgttagcag
     8101 ggcttcttga accaactagt ggtgaaatcg cggtcaacgg tcaagttgtc aatctcgact
     8161 ccccatctaa agcagctagc ttgggaatcg ggatggttca ccagcacttt atgttggttg
     8221 aagccttcac agtggctgaa aacatcattt taggtagtga attgactaaa aatggtgtgc
     8281 tagatatcgc tggagctagc aaagaaatca aggctctttc tgaacgttat ggcttagctg
     8341 ttgacccttc tgccaaggta gcagatatct cagttggagc ccaacaacgt gtagaaattt
     8401 taaaaacact ttatcggggg gctgatatcc ttatctttga cgaaccaacg gctgttttga
     8461 ctccatcaga aattgatgag ttgatggcta ttatgaaaaa tcttgtcaaa gaaggaaaat
     8521 caattatctt gattacccac aaattggatg aaattcgagc agtttctgac cgtgttacag
     8581 ttatccgtcg tgggaaatca attgaaaccg ttgaaattgc aggggctacc aatgctgatt
     8641 tggcggaaat gatggtagga cgttctgttt cctttaaaac agagaagcaa gcctctaaac
     8701 caaaagaagt ggttttgtct atcaaagatt tggtggtcaa tgaaaaccgt ggtgttccag
     8761 ctgttaaaaa tctgtccttg gatgttcgtg ctggagagat tgttggtatt tcggggattg
     8821 atggaaatgg tcagtctgaa ctgattcaag ccattacagg tcttcgtaag gttgaatctg
     8881 gtagcattga gctaaaagga gattcaattg taggcttgca cccacgtcag attacagaac
     8941 taagtgttgg gcacgttcca gaagaccgtc accgtgatgg cttgattttg gaaatgatga
     9001 tatctgaaaa tattgccctt caaacctact ataaagaacc acatagtaaa aatggaattt
     9061 tgaattattc aaatattact tcttatgcta aaaagctgat ggaagagttt gatgttcgcg
     9121 ctgccagtga attagttcct gcagctgcac tctcaggagg aaatcaacaa aaagcaatta
     9181 ttgctcgtga aattgatcga gatcctgatc tccttatcgt tagccagcca actcgtgggt
     9241 tggatgtcgg tgccattgag tatatccaca aacgcttgat tgaagagcgt gataatggca
     9301 aggctgtcct tgttgtcagc tttgaattgg atgagatttt aaacgtctca gaccgtattg
     9361 ccgttatcca cgatggtaag attcaaggta ttgtatcacc agaaacaacc aataaacaag
     9421 aacttggtgt cttgatggct ggtggaaact tgggaaagga gaagagtgat gtctaaaaaa
     9481 ttacaacaaa tttcggttcc cttgatttct gtatttctag gaattttact cggagccatt
     9541 gtcatgtgga tcttcggtta tgatgctatt tggggctacg aagaattgtt ctatacagcc
     9601 tttggcagtc tgcgtgggat tggagaaatc ttccgtgcta tgggtcctct ggtcttgatt
     9661 ggtcttggtt ttgcggttgc cagtcgagct ggtttcttta acgtcggact tcctggtcag
     9721 gctttggcag gttggattct cagtggttgg tttgcgctgt cgcatccaga tatgccccgt
     9781 cccttgatga ttctagcaac catcatgatt gccttgattg ctggtgggat tgtcggagcg
     9841 attccaggta ttcttagggc ctatctaggg acgtcagagg ttattgtaac catcatgatg
     9901 aactacattg tcttgtatgt agggaatgcc tttatccatg ctttccctaa agacttcatg
     9961 caaagtacag attcgaccat tcgtgttggg gctaatgcaa cctatcagac accttggttg
    10021 gctgagttga ctggtaactc acggatgaat attggtattt tctttgccat cattgccgtt
    10081 gcagttattt ggttcatgct caagaaaaca actcttggtt ttgaaatccg tgcagttggt
    10141 cttaatccac atgcttcaga atatgctggt atttctgcca agcggactat tatcctatct
    10201 atgattattt caggtgcctt ggcaggtctt ggtggagctg ttgaaggttt gggaaccttc
    10261 cagaacgtct atgttcaagg ttcgtcatta gctatcggat ttaacggaat ggcggttagt
    10321 ttgcttgcgg ccaactcacc aattggtata ctctttgcag ccttcctatt tggcgttctc
    10381 caagttgggg ctcctggtat gaatgcggcg caggtaccat ctgagcttgt cagcattgta
    10441 acagcgtcta ttatcttctt tgtcagtgtt cattacctta tcgaacgctt tgtcaaaccg
    10501 aaaaaacaag ttaaaggagg taagtaaaga tgtctattat aaccttgctc cccctcttgg
    10561 tgtcttctat gctgatttac tcagcacctc tcatctttac aagtatcggt ggtgttttct
    10621 ctgaacgtgg tggtgtggta aacgttggcc ttgaaggaat tatggttatg ggtgcctttt
    10681 ctggagttgt ctttaacctt gaatttgcag aacaatttgg agcagcaact ccatggctat
    10741 ccttgcttgt agcaggattg gttggtagtg ttttctctat catccacgca gcagcgacgg
    10801 ttcatttccg tgcagaccat gttgtcagcg gtacggtatt gaacttgatg gcgcctgcct
    10861 tggctgtttt cttagttaaa gttctttata acaaaggaca aaccgacaac ctaagtcaaa
    10921 cttttggacg ctttgatttc ccagtcttgg caaatatccc agcgattggt gatatcttct
    10981 tcaagtcaac tagtctactt ggttatctgg cgattgcctt ctcattcctt gcttggttta
    11041 ttctctttaa gactcaattt ggtcttcgtc tccgctctgt cggtgaacat cctcaagcag
    11101 cggatacctt gggaatcaac gtctacaaga tgagatattt agggattatt atttcaggtt
    11161 ttctaggtgg aattggcgga gcgatttatg cgcaatcaat ctcagttaac ttctcagtga
    11221 caactattgt tggacctgga tttatcgccc ttgctgcgat gatctttggg aaatggaatc
    11281 caatcggtgc tatgctttct agtctcttct ttggactttc acaaagtttg gctgttatcg
    11341 gttctcaatt gccgttccta caaggagtgc cagcggttta tcttcaaatt gcaccttatg
    11401 ttttgacaat tcttgtctta gcagccttct ttggaaaagc agtcgcacca aaagcagatg
    11461 gtatcaacta catcaaatca aaataagcat acaaaaaaac gtcagtccaa tgactgaact
    11521 gccccccaaa agttagacag aaaaaatcta acttttgggg tgtttttatt atgaaactaa
    11581 cttatgatga taaagttcag atctatgaac ttagaaaaca aggatatagc ttagagaagc
    11641 tttcaaataa atttgggata aacaattcta atcttaggta catgattaaa ttgattgatc
    11701 gttacggaat agagttcgtc aaaaaaggga aaaatcgtta ctattctcct gatttaaaac
    11761 aagaaatgat tcataaagtc tgacatgaag gctagactaa agatagagtt tctcttgaat
    11821 acggtctccc aagtcgtacg atacttctta actggctagc acaatacagg aaaaacgggt
    11881 atactattgt tgagaaacca agagggagag tacctaaaat gggacgtaag ccaagaacga
    11941 gacctgaaga gaggacagaa ttagaacgtc ttcaagcaga aaatgagtac ctgagagcgg
    12001 agaatgccat cctaaaaaag ttaagagaac tccgattgaa ggaggaaaaa gagaaagaag
    12061 aaagacagaa attgttcaag aattaatgac tgagttttcg ttagctcttc ttctaaaagc
    12121 cattaaacta gctcgttgga cctactacta tcacttgaaa cagctagata aaccagataa
    12181 ggaccaagag cttaaagctg aaattcaatc catttttatc gaacacaagg gaaattatgc
    12241 ttatcgctgt attcatttag aactaagaaa tcgtgcttat ctggtaaatc ataaaagagt
    12301 tcaaggcttg ataaaagtac tcaatttaca agctaaaatg cgacagaaac gaaaataaag
    12361 gagacgttgg taagaaggca gagaatctca ttcaaggcca atttgaaggc tctaaagcaa
    12421 tggaaaagtg ctacacagat
//
LOCUS       DBERMR010000059        12297 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.059, whole genome shotgun sequence.
ACCESSION   DBERMR010000059 DBERMR010000000
VERSION     DBERMR010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12297
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.059"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            8..1231
                     /locus_tag="ACL8A1_001826"
     CDS             8..1231
                     /locus_tag="ACL8A1_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HGS1202542.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPYVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTAIEYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNNPIWESFNLLNVIKRFNFPFIVKNNIDCMAIGQYLFNPHNTPDNFIFLHAGLGIY
                     TSFFTKEKIGASKNPYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFK
                     NSQLTVLKSLVKTEKDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPAD
                     KIYINSQLLNYQPFTHEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVA
                     FFIEHSNVLQDIISP"
     gene            2663..2947
                     /locus_tag="ACL8A1_001827"
     CDS             2663..2947
                     /locus_tag="ACL8A1_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202543.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVILLNKPAKLTNVQKLMEKYKHG"
     gene            2940..3188
                     /locus_tag="ACL8A1_001828"
     CDS             2940..3188
                     /locus_tag="ACL8A1_001828"
                     /inference="COORDINATES: protein motif:HMM:NF039330.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HGS1202544.1"
                     /translation="MDKMKPVFQALNKELIQENLTLTIICVGGYVLEYHGLRATQDVD
                     AFYDQNQKINEIIARVGKQFNLNIHEELWLNNLVAKQI"
     gene            3284..3502
                     /locus_tag="ACL8A1_001829"
     CDS             3284..3502
                     /locus_tag="ACL8A1_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202545.1"
                     /translation="MMSIREQDLQDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
     gene            complement(3869..6193)
                     /gene="pflB"
                     /locus_tag="ACL8A1_001830"
     CDS             complement(3869..6193)
                     /gene="pflB"
                     /locus_tag="ACL8A1_001830"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HGS1202546.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            6390..6681
                     /locus_tag="ACL8A1_001831"
                     /pseudo
     CDS             6390..6681
                     /locus_tag="ACL8A1_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721503.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
     gene            6923..8384
                     /gene="mobQ"
                     /locus_tag="ACL8A1_001832"
                     /pseudo
     CDS             6923..8384
                     /gene="mobQ"
                     /locus_tag="ACL8A1_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042188.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
     gene            8446..8865
                     /locus_tag="ACL8A1_001833"
     CDS             8446..8865
                     /locus_tag="ACL8A1_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HGS1202547.1"
                     /translation="MNEREKIIRLWFDMWLTQQDLGIDDIFLDDVIYIESWSPKYENC
                     QTVKHWFNEWNTRGKVLAWDIKQFFHKDNQTIVEWHFKNKMNEGKVEEFDGISLIVWT
                     ADNKIKALKEFGCNCNNYNPYKESETPLFRDEKVNWF"
     gene            9018..10079
                     /gene="dinB"
                     /locus_tag="ACL8A1_001834"
     CDS             9018..10079
                     /gene="dinB"
                     /locus_tag="ACL8A1_001834"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HGS1202548.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAMEIKDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKAVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGISYNKF
                     LAKMASDYQKPHGLTVILPEQAENFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYNLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILRAEEDIKKEL
                     TILSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSEKERGVRLLGITLTGF"
     gene            complement(10226..11071)
                     /locus_tag="ACL8A1_001835"
     CDS             complement(10226..11071)
                     /locus_tag="ACL8A1_001835"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HGS1202549.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLVATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGIAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            11327..12276
                     /locus_tag="ACL8A1_001836"
                     /pseudo
     CDS             11327..12276
                     /locus_tag="ACL8A1_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT         4304 a         2246 c         2213 g         3534 t
ORIGIN      
        1 gaaacaaatg attacaaaac aatcagcctt tttacaaaat agagccacta tactggagtt
       61 tctatatcga aatccagcaa catcccgtac agatattgtt aatgaaacag gacttactcc
      121 agcaacaacg actaacatca taaaagagct aagcgaacag tccctcatat acgaaactgg
      181 tgatgaattt agcgagtttt ccggatctgg aagacgtaga aaaactattt ctatcacaga
      241 taatattccg tatgttgttg gaggtattga aataaacgtc ctaggtatct ttctcagtct
      301 atgtgactta caagggaaaa ctcttttcga gacagaaatt ttgaatgaag attatcctat
      361 ttcagaaatc aattccacca ttaccaatat gataaaaaca gctatagagt acgtcccttt
      421 ggaaacaaaa ttacttggat ttggcttatc aatacctgga cattataaca aagactccgg
      481 aagtatcatt acaaacaacc ccatatggga atcttttaat ttattaaatg taattaaaag
      541 attcaatttt ccttttattg taaaaaataa tatcgattgt atggctatag gacaatacct
      601 ttttaatcca cacaataccc ccgataactt tattttccta cacgctggat taggtattta
      661 cacttccttt ttcacaaaag aaaaaatagg agcctctaaa aatccttata tcggagaaat
      721 tggacacacc attgtcgaat tgaatgggca atattgtgaa tgcggaaaaa aaggttgttt
      781 acaaacatat atttcggatg cttggttaat caaacacgcc caattattat ttaaaaattc
      841 ccaactaact gtactaaaaa gccttgtaaa gactgaaaaa gacattcatt tagacaccct
      901 tttaacggct tataatttag gcgactccgc tttacgtcaa caaattgata aaggagtcaa
      961 tttattagcc acttctattg caaatctcct cctcatcaat cctgctgata aaatctatat
     1021 caacagtcaa ttgcttaatt atcaaccttt cactcatgaa gtcagggata aaatccaaga
     1081 ccagctccac ttcgttccct ttactcgtaa tatagaaatt gaaattttac cttacaacaa
     1141 acatcgtgga agtataggag cttgtgcatt agctatcgtc gcttttttca tagaacatag
     1201 caatgtatta caagatatta tttcacctta atatattaga aatctataga cctgtttaaa
     1261 tcaactataa cctgtagtag atatctcgta tttagacaat atgaaaacaa gacgacttcc
     1321 atataggaaa ccgccttctc tctatgttga gtgatttata ttaaaataac ttttcttcta
     1381 gctgcatttt attattataa aaacattcat cataaccctg aacttaaata acgattttta
     1441 ttcaagatac atactcctag aataaacttt atatgaaatt ctcatttttg tttttacaat
     1501 tctccttagt taaatcttgt ttaatatatg ttttacatat agtatttagc gccacatagt
     1561 actgaactct ctccaaaaac ggttattcct ctttgaatag ggcgttatca caagaaaagc
     1621 atctccacgt ttcaacttca tatggctcaa aaacaatcaa ttgatgctaa aacctgtacc
     1681 tagatgtttc ggttcataaa accatgaaac tgtaaaagtg gatgaaattg atagcgatag
     1741 tcaaatcaag aggcatcata actctaaaaa gtcacaatat ataagttcat cctcggaaaa
     1801 atatcattct aattgttgaa atgcctacat gaaaagaaac gtcaaatact catgaaacaa
     1861 cgaatacagg tatcaaaact atgacaaaac aagtccctac atttactaaa gacactgctc
     1921 aactttacac ctgtaaatgg ttgttgtata ataaagttac aaagatgtac gaccacactg
     1981 ttgtaaatca tagtgttcgc gaatatatta ctgatagcat ttctacaaat acaattgaaa
     2041 aggctggata ggctttaaac gaaatatgtc agtgttttga cattcctagc cttcatatca
     2101 tttaaagaat tgtatagata aaattttttc caatacagac actcgtaaca actgcttcat
     2161 ttttctacca acatatttag gaacaggata cgatacaaga ggattaatcc atagatcagt
     2221 tctataccaa tctaagacaa ataagctaaa aaaacgattg ataataagca aatagattcc
     2281 aaattttctc tatctgctca ttttaataaa caatactagt gtaactatcc ttccagtcag
     2341 aagcttgtca aatcacaccg aaaattcttc taaaatttat ctcgttaggc aatcaagcaa
     2401 aaactcgacg atagtacaaa cattatcata caggattgac ttcctaaatt atatacttta
     2461 gtaaggtttt cggataagaa aaaaggttca ttttacattt ctaaacattc ttttctaaga
     2521 tgaaaaacag aatttttcga ttgtgattta aagcaacaag aagattttca gtatcatcct
     2581 atagatacga gctaattaag aaaaactaca tttttgaata taaactacaa taagataaac
     2641 taaatttcat aggaggaaga caatggattg gtacgattat atgatacagg catccaaaca
     2701 atcacaattc aacgcaagcc attggtttcg ctatttgcga aaagttattt ttgaagacta
     2761 ttcttattta acaaaccaag atgtagaaaa gttgctagac tccaaagaac taacccgttt
     2821 tcaaaaaatt agcttgaagt atgcctttca agagcatact ccaactcata aatatgtgat
     2881 tttattaaat aaacctgcta agttaaccaa tgttcaaaaa ttgatggaga aatacaaaca
     2941 tggataaaat gaaaccggtc ttccaagccc taaataagga attaattcag gaaaatctga
     3001 ctttaacaat tatctgtgtc ggtggttatg tcttagaata tcatggttta cgtgccacac
     3061 aagatgttga tgctttttat gaccaaaatc agaaaatcaa tgaaattatt gctcgtgtag
     3121 gaaaacagtt taatcttaac atccacgaag agttatggct caacaatctt gttgcaaagc
     3181 aaatatgaac aaacaacctc ccttaagttt atgcgaatcc ttgtactcat ttgagaatct
     3241 gaccgtttta gtagtcccta ttgagtatgt gttaggaatg aagatgatga gtatcagaga
     3301 gcaggatttg caagatattg gtgcaattat taaatacaaa aactttcatt caccattcga
     3361 tacctttaaa tatttaaaag acatgggatt tgatactatt gacctctctg ttctattaga
     3421 aggttttagt tatgcttatg gtatggattg gttagaaaaa ttttttaaag aaaatcaaga
     3481 taaacttcgc gaattttatt aacagtatca aacaagatga ttgtcaatta gccagtcgtc
     3541 ttatttttat gatggaatga aataaaatcg gaacaaatca cttagagaat tccagtcaat
     3601 ttctagcact gtttgagacg tttcagcgaa ctatagtaaa atgaaataag aataggacaa
     3661 atcgatcagg acagtcaaat cgatttctaa gaatgtttta gaagtaaagg tgtactattc
     3721 tagtttcaat ctattatatt tcagattcgg tccactattc cttcccaaaa acattgcata
     3781 ttgcgccgaa ggaattgctc acactatccc aagcacaaaa ccctcagtcg attgaccgag
     3841 ggttcctttt cattattcaa aaacttgatt agctcaatgc atccaaggca tcatccattg
     3901 aaagaacttc gtggaagaca cgttgtgtca attcagtttt ttgttctgga gtgaggtatt
     3961 tagtgtttac acagtatcca gagatacgta cgataacgtc ttcgcctgac atgatctttt
     4021 cgtaaacatc gttcaagtcc ataacgttca agttaacgtg ttgtccaccg ttttcgaagt
     4081 aaccatcaag gattgttacc aagttatcaa cttgttcatc acgagtctta ccaagagcgc
     4141 gaggtgatac ttgtgtagtc aatgagatac cgtcagctgc ataactaaag tcaaggctag
     4201 aaagtgagtt caagttttgc aaccaaccac ctttagcttt gttagatggg ttagcacctg
     4261 gtgagaagaa ttcaagttta gacaagttca cagaaccatc ttcgttgagg tatacacctt
     4321 tgtgaactgg tgagttacca gtttgtttag agtaagcaac gttagatgtg attgtcaaaa
     4381 gtgatactgt agcttctgcg tctttgtata gtttgtggct acgtagacga gttgtgtaag
     4441 cttcgatcaa ccattctgcc aattcgtttg aacgtgggtc atcttcaccc cagcgtgggt
     4501 agtcaccgat tgtttcgtaa tcgtagatgt agccatcttc gtcacggatt ggtttaactg
     4561 tagcgtattt gatagctgac aatgtatcaa cagtgttagc aaatccacag ataccgaatc
     4621 ccatgttggc acgttgttta gttggcaaga aggccatttg aacagcttcg tagttgtacc
     4681 tatcagtcat gtagtggatg atgttcaagg catctacgta agtgtcagtc aaccagtcaa
     4741 gagatttttc aaagttcgct ttaactgatt caaattcaag aacttcgtca cggattggtt
     4801 cgatatcaaa tactttgtag tctttgtgaa catcgtcgta accaccattc aaaccagtaa
     4861 gaagggcttt aagaacgttt acacgagcac cgaagtactg gatgttgtgg cgttgttctt
     4921 cattttctgg atcaagtgga gacacacagc atgagataca gctcatttca ccatatccgt
     4981 ctttagccat tgttgttaca ccttcgtatt ggatagaaga gtgtttgtgg ctcatgtgca
     5041 tacagtagcg acggaagttg tatggcaatt tgtcagtcca aagaactgtc aagtttggtt
     5101 ctggtgagtt accgatgttg tcaagagtgt tcaagaaacg gtagtccatc ttagtaacac
     5161 ggtgacgacc gtcgttaccc ataccagcca tagaagttgt gataaaggtt gggtcacctg
     5221 agtacaattg gtcataagct ttggtacgag caaatttaac tgtacgaagt ttcataacga
     5281 aatcatcaac gaattcttgg atttctgatt cagtaaatgt accacgagca aggtcacgtt
     5341 ctgcaaagat gtccaatacg attggtacac gacctagaga tgtagcagca ccgttaatca
     5401 cacggcagac agccatgaaa gcaatgttaa cccattggat tgcttctttc acgttcatcg
     5461 ctggtttgcg aacatcaacc ccgtaaaggt cacccaggcg aacaacttgt tgcaatgctt
     5521 ggtattgaag gtttacttct tcacgaagac ggattgtttc ttcatcgatt tctttgattg
     5581 cattccagtc gtttactttt tcttgcatca agtagtctgc accgtaaaga gcaagacgtg
     5641 cgtaaacacc gatgatacgt ccgcgtgagt atgcatctgg aagaccagtt acagtgtgtg
     5701 catgacgagc gcgacgaata tttgaagtgt aggcacggaa aataccgtcg ttaactgttg
     5761 ttacatattt agtgaagatt tcgtgaacag ctgggtctgg ttcgtatcca ttttctttta
     5821 aagtagtttc agccatacgg ataccacctt ttggcatgaa gttcaatttg aagagttcat
     5881 cattttggat accaaagata acttcatttt ctttgtcgat aaatccagca gggatatcag
     5941 cgatagatgt tggacgagtg tccattggga aacgagtttc ttcgtagtgt gctttagttt
     6001 cttctacaat tttcttgatg tgaagtgaac gctctgttgg tcctgcaagg aagctttcgt
     6061 ctccatcata aggtgtgtag ttagcttgta caaagcgtga cacacttgct ttttctttcc
     6121 aatctacgcc tttgaagcct tcccaagctt tgtcaaaaat atcttgtgct tcaacaactg
     6181 tcttaacaac catattaatg tcctcttttt tctagtaaca gtcatctgtt acattcatga
     6241 gacaagtata ccatatagta accgatttca acaagtgaaa aatccctatt tttacacttt
     6301 cttttctaaa acagtctata ttttatttca aactgtatta tatttttgaa aaaataaagt
     6361 ccttttttct ttttttcaga aaaaagggta taataaaaga aaataagcag ttacaaaaca
     6421 aaagaaaaaa gctcattagt cagcagaaag aagaggaacg aaaaaaaaag agatagacgg
     6481 ctttatgaaa aaggaggagt ctttgaaagt atctttaccg aaagcaagaa cttaaccaaa
     6541 gatgaatttt atcagctaat tacattccaa aatatcaaag aagcagtcaa tcaaaaaatc
     6601 ctaaaaatta ttgagaagcg agaacaatgc gaaaatcaaa acacagaaaa ccaagaggaa
     6661 gaaatagaaa cagaggaata gtcccttgta tacaagggcg cacttataca ccctaaaggg
     6721 tgtgtgcgtt ctccgaaggc tcttagcaga gggcatatca gctaacgctg atacagggga
     6781 gctacactcc cctacgaaaa taaatttcct accccttgta tacttcccaa aagaaatcgg
     6841 ataaaaagct atccgatttc tttaagaagt cttattcatc gccacatcaa agtattagaa
     6901 aaacgaaagg aggttttctc ttatggcgat atatcatctt tgcataaaga ttatctcaat
     6961 aggtaaaggc aaaagtgcag tagtagcttc cgcctatcga agtggtgaaa agataaaaaa
     7021 tgaatatgac ggaatagtcc atgactttac aagaaaaggt ggaatagcac ataccgaaat
     7081 cctattacca caaaatgcac ctgaagaatt ttcaaaccgt tctgtattat ggaacagtgt
     7141 tgagaaaata gaaaaaagta aaaatgcaca acttgcaaga gaaatagcat tacctaaaga
     7201 attaagcaga gaaaaacaaa taaatcttgt aagagagtat gtaaaagaaa attttgtgaa
     7261 agttggtatg tgtgccgata ttgcattaca cgataaaaat gacggaaacc cacacgcaca
     7321 tatcctactt accatgcgac cgttaaacga agataaaaca tggggagcaa agtcaaaaaa
     7381 agaatatatc cttgatgaaa acggagagaa agtaaaactt aaaaatggaa attataatta
     7441 taaaacaagg aaaatcaata cagtggattg gaacgaataa tacaaatcgg aagagtggcg
     7501 aaaagcatgg gaagatatta caaacaaata tttggaagaa aatagcatac aggaaaaagt
     7561 agatcaccgt tcctatcaaa gacaaggtat agaacaaata ccgaccattc atttaggcgt
     7621 atcagccacc caaatggaaa agaaaggcat agccacagac agaggaaata ttaaccgaga
     7681 catcaaacat ccaaaacagg attttaagag aaatttcagg atgaataaaa gccttactaa
     7741 attggataag gggaatacgc aaagaagaaa aaacagaaat tgataatctc aaatccaccc
     7801 tcccacccaa agaaaatttg ctatccgttt ttgaaaatct tatccgtaaa aaagcagata
     7861 gcaataacac agatttagga aaatacatcg aaagctatca gttcttaaaa gagaaaaata
     7921 tcatttccgt atctgaactg aaagagagta ttaccgattt acgagacaaa aattataaga
     7981 ccacaagagc tttgaaagat accgaaaagg agattgatga taaaaccaaa cttattgacc
     8041 aagccgaaaa atatttgaag cataaagaca cctacaaagc ttataccaag ttaaagaaaa
     8101 acaaacaaga tactttctac aatgaacaca ccgcagagat tattttattt gaaagtgcaa
     8161 ataaatatct gaaagagcat ttaggtgaaa gcaagacctt aaatatatcc aaatggaaat
     8221 cggaacttac cactttgaag aaagataaaa agagcctata tagtcaaata ttagaaatac
     8281 gagaggaagt agaacaggct gaaaaagtta agacctgtat agagcagtta caggaacaag
     8341 aaaaacaact atcacaggta aagagaaatg agttagacct ataaaactaa gtgaaaaaat
     8401 ggtataattg tatataatta tttgattttg gaggtgctta aatttatgaa tgaaagagaa
     8461 aaaatcattc gtttatggtt tgatatgtgg ctcacacaac aagacttagg tatagatgat
     8521 atttttttag atgatgtaat ttatattgag agctggagtc ctaagtatga aaattgtcaa
     8581 acagtaaagc actggtttaa tgaatggaat acaagaggaa aagtgcttgc ttgggatata
     8641 aagcaatttt ttcataagga taatcaaact attgtagaat ggcacttcaa aaataaaatg
     8701 aatgagggga aagttgaaga atttgacggt atttctttga ttgtttggac agccgataat
     8761 aagataaaag cattgaaaga gtttggctgt aattgtaata actacaatcc ttacaaagag
     8821 agtgaaacac cattgtttag agatgaaaaa gtgaattggt tttaaggtga ttactatgga
     8881 tatagataga tttttcaaaa cagttttatc acaaaatgaa gaagaactta ggaatttttt
     8941 caaaaaaaaa tgctgttata aaatggcact gcacaaatga gttttttacc ttagaagagt
     9001 atataaaggt gctaggcatg ttgatttttc ctttgttaaa tgatttgtca agaaaaatca
     9061 tccatattga catggatgcc ttttttgctg cgatggaaat caaggataat cctaaactca
     9121 gaggaaaacc tgtcattatt ggaagcgacc ctcggcaaac aggtggacgg ggagtcgttt
     9181 ctacctgtag ctatgaggct cgagcttttg gtgtccattc tgccatgagt tccaaggaag
     9241 cttatgaacg ttgtccccag gccgtcttta tctcaggaaa ttatgagaaa tacaaagctg
     9301 tgggactcca gattcgagct atttttaagc gctatacaga tttgattgaa cccatgagca
     9361 ttgacgaagc ctatttggat gtgacagaaa ataaactcgg tatcaagtca gcggtcaaaa
     9421 ttgctcgcct cattcaaaaa gatatctggc aagaactcca tctaactgct tctgccggta
     9481 tttcctacaa caagttctta gctaaaatgg cgagtgatta tcaaaaacct catggtttga
     9541 cagtgattct acctgaacag gctgagaatt ttctcaaaca aatggatatt tccaaatttc
     9601 atggagtagg aaaaaagaca gtagaacgtc ttcatcaaat gggcgttttt actggtgctg
     9661 atttacttga agttcctgag gtgaccctaa tagaccgttt tggtagacta ggctataatc
     9721 tgtatcgaaa ggctcgtggc attcacaact ctccagtcaa atccaatcgc atccgtaaat
     9781 caatcggcaa ggagaaaacc tacgggaaga ttctccgtgc tgaggaagat atcaaaaaag
     9841 agctgactat tctatcagaa aaagtcgctc tcaatctaca tcaacaagaa aaagctggaa
     9901 aaattgtcat tttgaaaatc cgctacgagg acttttcaac tcttaccaaa cgaaaaagtc
     9961 ttgctcaaaa aacacaagat gctagtcaga taagccaaat agccctgcaa ctctatgaag
    10021 aattaagcga gaaagaaaga ggtgtccgcc tattgggaat taccctgact ggattttaaa
    10081 gctcaatgaa aatcaaagaa caaactagga agctagccgc aggctgctca aaacactgtt
    10141 ttgaggttgc agataaagct gacgcggttt gaagagattt tctaagagta taaaaccttg
    10201 aagagttgcc ccttcaaggt ttttcttata caaataaacg gacaaaactg taaagtagca
    10261 aaacactacc aagcacgata cggtatttac caaaaagggt gaagtcgtgt tttttcacat
    10321 agctggtcaa gaagcgaata gccaccatgc tgaccgcaaa agctactccc atcgcgacca
    10381 agagcaaaaa caattgccca aagctcaaga gttctcctgc ttttacaaat ttgaaaatct
    10441 ttaaggcact agctccaaac ataacgggaa tcccaagata gaaggtaaat tctgtcacaa
    10501 ctgaacgact ggttccattt aacaaaccac cgacaatcgt tgcacctgaa cggctagtcc
    10561 ctggtaaaag agcaagaact tggaagagtc cgatatagaa agcggtcgta taaggaagct
    10621 tgtccaactc tgttacactt ggctcgatag cacgcgcttt attgcgcttt tccaaataga
    10681 tgaaggcaat cccgtagata atcaacatga gagcaactga aaccatgtta tggaagtggg
    10741 tatcaaacca atcatcaaat ttaaagacac caagtaaagg caaagtggca accaagacct
    10801 tcaaccacag tctccaagtc ttacgaactt cctgtttgtc cttagtcggt ttaaaaggat
    10861 tgagcttgtt aaaataaatc accataactg ctaaaatagc accaagctga atcacgacat
    10921 taaacatgga cataaaggct tcattttgat tttggtattg gataaactcc tctgctaaaa
    10981 tcaaatgacc tgtactggaa atcggcaacc attccgtaat tccttcaaca atcccgaaga
    11041 agatagattt taaaatttca ataagataca tagattactc cctatattat atttagcgca
    11101 gttagctact gccaaagccc aagtggtata cttggaataa gccactgtgg attagtttat
    11161 tccctccaat cgcttgacga tttccgaaag gctctaacca cagttttttg tttcaattgg
    11221 taatgagttt gataacgcat agacgtttta tctcgtctaa ggtaacaagg caaaaaattc
    11281 gtggataacc aagtagactt gaaatactac atctggaggt actcccatgc tttatgtggg
    11341 cattgatgtc actaaaaata aacacgatgt tacagccttg aatgttccag gaaaaactgt
    11401 tcttaaacca ctcacttttt caaataataa agctggtttt gaactcttag atctgtctct
    11461 tcgacaactc aaccaagact gtctcatcgc tcttgaaaat accggacatt atgcctttaa
    11521 tctactgaac ttcctgcatg aacaaggata taaagtttac acctgcaatc ctttgctcat
    11581 caaggaattt gccaaatcat tatcacttcg caagactaaa acggataaga aagacgctca
    11641 tggcatcgct cttaaacttc tttctgaccc caatcgtgaa caatttcaac acgataatcg
    11701 gcaagtagaa ctaaaaatac tggctagaca tattcatcgt ctcaagaaaa acagtctgat
    11761 tggaaagtac aatacactcg ttgtcttgat atcatctttc ctgagttgga taaaatcgtt
    11821 ggaaagcatt cagaatatac ctaccaactc ttgacacgct accctaatcc tcagaaaagg
    11881 attgaggcag gatttgataa gctgatagaa attaagcgat tgaccgcttc taaaattcag
    11941 gatatcctct cagtcgcacc tcgttctatc ggaacaacat ctcctgctcg tgaattcgaa
    12001 atcatcgaaa acatcaaaca ttacaagagg ctcattgaca aggcggaaaa atgtgtcaat
    12061 gacttgatgt ctgagttcaa ctcggtcatc acgacggtta ctgggattgg gaatcgttta
    12121 ggggcggtca ttttagccga gattcgaaat attcatgcct ttgataatcc tgctcaatta
    12181 caagctttcg ctggactgga ttcttctatt tatcagtcag gtcagattga tttagcagga
    12241 agaatggtca aacggggttc ccctcatctg cggtaggtac tcatacaagc tgccaaa
//
LOCUS       DBERMR010000060        12253 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.060, whole genome shotgun sequence.
ACCESSION   DBERMR010000060 DBERMR010000000
VERSION     DBERMR010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12253
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.060"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(255..2513)
                     /locus_tag="ACL8A1_001837"
     CDS             complement(255..2513)
                     /locus_tag="ACL8A1_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGS1202550.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2742..2972)
                     /locus_tag="ACL8A1_001838"
     CDS             complement(2742..2972)
                     /locus_tag="ACL8A1_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1797 family protein"
                     /protein_id="HGS1202551.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            3162..3845
                     /locus_tag="ACL8A1_001839"
     CDS             3162..3845
                     /locus_tag="ACL8A1_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGS1202552.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSEIVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            3845..4579
                     /locus_tag="ACL8A1_001840"
     CDS             3845..4579
                     /locus_tag="ACL8A1_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGS1202553.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKVIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            4733..5590
                     /locus_tag="ACL8A1_001841"
     CDS             4733..5590
                     /locus_tag="ACL8A1_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HGS1202554.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLAHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            5732..6604
                     /locus_tag="ACL8A1_001842"
     CDS             5732..6604
                     /locus_tag="ACL8A1_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HGS1202555.1"
                     /translation="MKVINQILLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEPMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDASGENLVKQVFVNLSQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DALAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERVSKDKLV"
     gene            6886..7569
                     /gene="rpiA"
                     /locus_tag="ACL8A1_001843"
     CDS             6886..7569
                     /gene="rpiA"
                     /locus_tag="ACL8A1_001843"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HGS1202556.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            7583..8794
                     /locus_tag="ACL8A1_001844"
     CDS             7583..8794
                     /locus_tag="ACL8A1_001844"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019318858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HGS1202557.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEADIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            8796..9338
                     /locus_tag="ACL8A1_001845"
     CDS             8796..9338
                     /locus_tag="ACL8A1_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HGS1202558.1"
                     /translation="MTRYALLVRGINVGSKNKVVMAELHQELTNLGLEKVESYINSGN
                     IFFASIDSKAQLVEKLETFFAVHYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYQKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            9354..10163
                     /locus_tag="ACL8A1_001846"
     CDS             9354..10163
                     /locus_tag="ACL8A1_001846"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HGS1202559.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIRFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKIDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            10639..11034
                     /locus_tag="ACL8A1_001847"
     CDS             10639..11034
                     /locus_tag="ACL8A1_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HGS1202560.1"
                     /translation="MLDSKEELKESENDAPKLETPLREEPRLAPQTLPEASEVLENKR
                     EESKVEITEPAQADDIRKVVGELAKDISITKLYMTGHSLGGYLAQIAAVEDYQKYPDF
                     YNHVLRKVTTFSAPKVITSRTVWDAKNGF"
     gene            11044..11619
                     /locus_tag="ACL8A1_001848"
     CDS             11044..11619
                     /locus_tag="ACL8A1_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HGS1202561.1"
                     /translation="MESRKLAVSGKIKHYVVDNDNVVTPLIHNNRDIVTFTGNSRFKH
                     RSRGYFESPMNDIPNFNIGKQATLDKHGYRDPKLDKARFFKKQALPQSSSQPSAEPME
                     NIALGKQVTQSSTAFGGDARRAVDGKVDGNYGHNSVTHTNFQSKPWWQVDLAKEETIR
                     QINIYNRTDTAQDRLANFDVILLDSSGKEIE"
     gene            11644..12156
                     /locus_tag="ACL8A1_001849"
     CDS             11644..12156
                     /locus_tag="ACL8A1_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-activated cytolysin C-terminal
                     domain-containing protein"
                     /protein_id="HGS1202562.1"
                     /translation="MSAQITINHKKVRYVRIELEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGC
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
BASE COUNT         3619 a         2240 c         2774 g         3620 t
ORIGIN      
        1 tgcaatacct tttacgaggc tcttttgtcc tgctcttgtt tcaattgact atagcagatt
       61 gaaataagat gagaacaaat cgattgggaa agtaaaatta atttctataa atgttttagc
      121 aattgtttcg tactatttca gattcagtct actatatatc ataatggaca aagggcaagc
      181 tgtcaagaaa ttacagttat tttagtaaaa aaagaagcag aaaaatttca ttttctactc
      241 cttttctata ggatttattt ttctttttca gaacttttaa cttcagcttt tttggcagat
      301 ttaatctgaa tgtttccctt gctagtcata actgctttga gatctttttc gcttggattt
      361 tcaaggtagt agtcagtgat tgtgtcctca atatagtctt gaatagtccg acgaagtggg
      421 cgtgctccca tttttggatc ataacctagg tcaaccaact tttccttgac cttgtcagtt
      481 acatccaaac gaatgttgtt gctagagagg cgcttgttaa catctgctag catgagctcg
      541 acaatctgaa ggaggttatc cttgctgaga gccttaaatt cgataatgcc atcaaaacgg
      601 ttcataaact ctgggctaaa gaagttaccg agttcaccga ggacagaatt ggtacgtcct
      661 tctctagcag caccaaatcc aacgctagct tcggtctttc ctgtacctgc atttgaggtc
      721 atgataatga tggcatcctt gaagctaacg gtgcgtcctt gcccgtctgt caaacgacca
      781 tcgtccaaga cttgaagaaa catgtgcata acatctgggt gagctttttc cacttcatcg
      841 agaaggatga gagaatatgg attgtggcga actttttcag ttaattgacc agcctcatca
      901 tagccaacat aacctggagg agcgccgacc aacttagcca cactatgttt ttccatgtat
      961 tcactcatat caaagcgaat catactatca gcagaaccaa aaagttcgat agccagttgt
     1021 ttggaaagtt ctgtcttacc gacaccagtt ggcccaacga agaggaagct tccgattggg
     1081 cggttagggg taccaagtcc gacacgatta cggcgaatag ccttggcaat cttatcgact
     1141 gcatcatctt ggccaataac atgagacttg agatcttcgg ctagatggat gagttgagat
     1201 tgttctttct ctttcaaatc accaacaggg atattggttt tctgctcgat aatgtgctca
     1261 atagttttct cgctgatgat aggagtatcc tggtctgtga tctttttctt ttgcatttcc
     1321 ttatacttgg caatctggtc gcggaagtag gccgccttct caaaatcttc ttctcgtgta
     1381 gcttgagact tgagattttc agcctcaatc aagcgctgat caattacttt aggatccaca
     1441 aaattcaagg tcaagttcat cttagaacca gcttcatcta ggaggtcaat ggccttgtca
     1501 ggcaagaagc gatcttggat gtagcgattg gaaagagttg cagctgcttc aatcgcagca
     1561 tctgtatatt gaacgtggtg gtaatcttcg tatttctttt gaatcccttt gagaatagtg
     1621 attgtttcgt ccaccgttgg ttcatcgact ttaacaggct gcatacgacg ctcgagggca
     1681 gcatcctttt caatgatacg gtattcattg agggtagtag caccgactag ttgcagttct
     1741 ccacgagcaa gggctggctt gaggatattt cctgcgtcca tattaccatc actcgcagaa
     1801 ccagcaccaa caatttcatg gatttcatcg ataaagagga tgatgtcttc acgtttgcga
     1861 atttcttcca tgagtttttg catgcgttct tcaaattgtc ctcgaatccc cgttccttga
     1921 actaagctaa ccacatccag acggatgact tgtttacctt ggagtttatg tggcacatcg
     1981 ccatcgacaa ttttctgagc tagaccttcg acaacggccg tttttccgac accaggttca
     2041 ccgataagga caggattatt cttggttcta cgattgagaa tctcgatgac acggataatc
     2101 tcatcgtcgc gcccaataac ggggtcaatg tctccacgac gggcaatttc agttacatta
     2161 ataccaaatt cttccagcag gcctttttct tggctaggtg gcggagtttg agcagatcca
     2221 cgattttggg aaccataacc gccgtttcca ccgtaacctc cacctgattg ggttggggga
     2281 ataggaggag tattgctaga aggtctgaaa ttgtttagat cattgaagaa atcaccaaag
     2341 ggatcgaagt cacgattgtt cagatccgtc atacctttga agaggctatt gttaggatct
     2401 gttttgataa tcttatagca gttttgacag aggtcaattt gtttttgttt tccattgaga
     2461 ttggtgtaaa gatgaattgt tgagtcgtta attttacagt tttgacaaag catacatcta
     2521 cctcatttct ttctttagcc tgacttgttt aaaggtcaaa aatagtcaaa ttctatactc
     2581 tcattataac aggattgggg tgtaaagcaa gtaaaaagat tgaagctttg agaagttgtt
     2641 acaatgaaaa tttttgtgaa tttggactat aaaaaaatcc tatttgtgag acaaaaagga
     2701 ttttacttag acatgcaggg acaatcccag cttgttttag attagtagag aagttcgtaa
     2761 atttccattg cgattaagtc aatgctatca aacgtatacg tttcgcgatc ttctacgtca
     2821 cgaagggtaa agattgggcc gttttcttcg tcgtggctgt acgcaacttc tgcaacaaca
     2881 actccttcgc gttcaaaatt acgttttaaa tttccgccat cgtttgccat tgcttcaagg
     2941 cggttgatga ttctaaccaa atgtgattcc attttgattc tccttcattt cttatcgtta
     3001 ctattttacc ataaaggagg ccaacttgac tagaaaaaac taagatttct cgctaattct
     3061 gctagtttaa agatttttaa ataaattgga taaaaaattt aaattttaat tcaaaacatg
     3121 ttataatcat acagtattct attttagaaa gcagtgtgac tatgaatttt tcttttttac
     3181 ctaagtattt accttatttt aactatgggg ctgttgtgac gattcttatt tctatctgtg
     3241 ttatcttttt gggaactatt ttgggtgttg tcttggcttt tgggcaacgt tcaaagttta
     3301 aaccgcttgt ttggttggcc aacttgtacg tttggatttt ccgtgggaca ccgatgatgg
     3361 ttcaaattat gattgccttt gctcttatgc atatcaatgc tccgactatt cagattggaa
     3421 ttttaggtgt tgatttttcg cgtctgattc cagggatttt gattatctct atgaatagtg
     3481 gtgcttatgt ttcggagatt gttcgtgccg gaatcaatgc ggttccaaaa ggtcagctag
     3541 aggcggctta ttcgctaggg attcgtccta aaaatgcgat gcgttatgtg attttgccac
     3601 aagcagtcaa aaatatcttg ccagcattgg ggaacgaatt tatcaccatt atcaaggaca
     3661 gctccctctt atcagctatt ggggtcatgg agttgtggaa tggggctaca acagtttcta
     3721 caacaaccta tctaccttta acaccacttt tatttgcagc attttactac ttgattatga
     3781 cctctattct gacagtagcc ttgaaagctt ttgaaaaaca tatgggacaa ggagataaga
     3841 aataatgaca gaaaccttga taaaaattga aaatttacat aaatcttttg gaaagaatga
     3901 agtattgaag ggcatcaacc tcgagattaa aagaggagaa gttgtcgtta tcatcggtcc
     3961 ttcagggagt gggaaatcta ccttgcttcg ctctatgaat ttgttggagg aagcaaccaa
     4021 ggggaaggtt atctttgagg gagtcgatat tacggacaag aagaatgacc tgtttgccat
     4081 gcgtgagaag atgggcatgg ttttccaaca atttaacctc tttcctaata tgactgtgat
     4141 ggaaaatatt accttgtcac ctatcaagac taaaggtgaa agcagggaag ttgctgagaa
     4201 gagagcccaa gagcttttgg aaaaagttgg tttgccagat aaggcagacg cttatccaca
     4261 gagtctgtcg ggtgggcaac aacaacggat tgccatcgcg cgtgggttgg ctatggaacc
     4321 agatgttttg ctctttgacg aaccaacttc agccttggat cctgaaatgg taggtgaggt
     4381 attggctgtt atgcaagacc ttgccaagtc agggatgact atggttatcg taacacatga
     4441 gatgggattt gcccgtgagg tggcagatcg tgttatcttt atggcagacg gtgtggttgt
     4501 tgaagatggg acacctgagc agatttttga acaaacccaa gaacaacgga ctaaagattt
     4561 cttgagtaag gttttataat ctattttaaa atttcaagac aagattagtg aaaagttcaa
     4621 ccagagcttt ttcttatagt ttgaaactat aggataacct aggaaagaag tgttagaggg
     4681 cactagcagt ttttggtata atggaagata tttgaaagaa aagagaagtg atatgacaca
     4741 gattattgat gggaaagctt tagcggccaa attgcagggg cagttggctg aaaagactgc
     4801 aaaattaaag gaagaaacag ctctagtgcc tggtttggta gtgattttgg ttggggataa
     4861 tccagccagc caagtctacg ttcgcaacaa ggagaggtca gctcttgcgg ctggttcccg
     4921 tagcgaagta gtgcgagttc cagagaccat tactcaagag gaattgttag acctgattgc
     4981 taaatataat caggatccag cttggcatgg gattttggtc cagttgccat taccaaaaca
     5041 tatcgatgaa gaggcggttt tattagccat tgacccagaa aaggatgtgg atggtttcca
     5101 tcccctaaac atgggacgtc tttggtctgg acatccagtc atgattccct cgacacctgc
     5161 aggaattatg gaaatgtttc atgaatatgg gattgacttg gaaggtaaaa atgcggtcgt
     5221 aatcggtcgt tcaaatatcg ttggaaaacc tatggctcag cttcttttgg ctaaaaatgc
     5281 gacagtaacc ttggcccact cacgaactca taatcttgcc aaggtggctg ctaaagcaga
     5341 tattcttgta gtcgcaatcg gccgcgccaa gtttgtgact gctgactttg tcaaaccagg
     5401 tgcggttgtc attgacgttg ggatgaatcg agatgaaaat gggaagctct gtggagatgt
     5461 tgattatgag gcagttgcac cacttgctag ccatatcacg ccagtccctg gaggtgtcgg
     5521 tcctatgacc attaccatgc tgatggagca aacttatcaa gcagcacttc ggacattgga
     5581 tagaaaataa gagaaacatt cgattaatag agtgtatttt ctatagctat atctaaaatg
     5641 gcagaaatga atattaaatt ttagatataa gattacaaaa ggaggtctgc gtctcctttt
     5701 tattgtataa taaaagtgag aggaaaaaag gatgaaagtg attaatcaaa tcttattaga
     5761 aaaagttatt attgaacgtt ctcgttcaag tcataaagga gattatggtc gtctgctgct
     5821 gcttggtggt acttatcctt atggaggtgc catcatcatg gctgctttag cagctgtaaa
     5881 aagcggtgca ggattggtga ccgttggaac ggacagggaa aatatcccgg ctctgcacag
     5941 ccatttacct gagcctatgg ccttttctct tcaagaccag caattgttaa aagagcaatt
     6001 ggagaaggca gaagttgtct tgctggggcc tggtttacga gacgatgctt ctggagaaaa
     6061 tctagtaaaa caggtctttg ttaatttaag ccaaaatcag attttgattg tagacggagg
     6121 tgccttgacc attcttgcta ggacaagttt gtcatttccc tcaagccagc ttatcctaac
     6181 tcctcaccaa aaagaatggg aaaaattatc tggaattacg attgaaaagc aaaaggaaga
     6241 tgcaacggct agtgttctga cttccttccc tcaaggaaca attttggtgg agaaaggccc
     6301 agctactcgt atttgggaag ttggccaatc tgattattac cagttacagg ttggtggtcc
     6361 ttatcaggcg actgggggaa tgggagatgc tctggctggg atgatcgcag gatttgtagg
     6421 ccaattccga caggccagtc tctacgagcg tgtggcagta gcgacccatc ttcattcagc
     6481 catagcccaa gaactatctc aagaaaatta tgtggtctta ccgacggaaa tcagcaggta
     6541 tcttcccaaa ataatgaaaa ttatttgtca acaagagagg gtaagcaaag ataaacttgt
     6601 ttaaaataga cagataaatc tacttccttc tcttaatgta tgattcttga aatgcgcctg
     6661 ctttaaggct aaactagacg tatcaaagtt aagagtttgt ttttgtaact ggaaagagat
     6721 gaacaaatca aatacataaa agataaactc atttttattc gtttggtaaa aataaaatgc
     6781 atatttttaa agaaagattg atgaaaaatg tagatttgat gtttttactt gaataaaaag
     6841 cggtttcata gtaaaataag aactgagaat gatggaggta aatcggtgga aaatctgaag
     6901 aaaatggcag gtatcaaggc tgctgagttc gtgagcgatg gaatggtcgt tggacttgga
     6961 acaggctcga ctgcctatta ttttgtcgaa gaaatcggtc gtcgtatcaa ggaagaagga
     7021 ttacagatta cagctgtaac aacttctagt gtgaccacta aacaggcaga aggccttaat
     7081 atcccgctca agtctattga ccaagtagac tttgtcgatg tgacggttga tggcgcggat
     7141 gaagtggata gtcagtttaa tggaatcaaa ggcggtggtg gtgcccttct catggaaaag
     7201 gtggtcgcaa caccatcaaa agaatacatt tgggtggtgg atgaaagcaa gctggtcgaa
     7261 aaactaggtg cttttaaatt gccagtagaa gtggttcagt atggtgcaga gcaggtcttt
     7321 cgtcattttg aacgagctgg ctacaaacca agtttccgtg aaaaagacgg ccaacgtttt
     7381 gtgacggata tgcagaattt tatcattgac ctcgccttgg atgtcattga aaatccaatt
     7441 gcttttggac aagaattgga ccatgtcgtt ggtgttgtgg agcatggttt attcaaccaa
     7501 atggtggata aggtaatcgt tgctggacga gatggagttc agatttcaac ttcaaaaaaa
     7561 ggaaaataga agggggcata agatgtctaa atttaatcgt attcatttgg tggtactgga
     7621 ttctgtagga atcggtgcag caccagatgc taataacttt gtcaatgcag gggttccaga
     7681 tggagcttct gacacactgg gacacatttc aaaaacagtt ggtttgaatg tcccaaacat
     7741 ggctaaaata ggtcttggaa atattcctcg tgaaactcct cttaagactg tagcagctga
     7801 aagcaatcca actggatatg caacaaaatt agaggaagta tctcttggta aggatactat
     7861 gactggacac tgggaaatca tgggactcaa cattactgag cctttcgata ctttctggaa
     7921 cggattccca gaagaaatcc tgacaaaaat tgaagaattc tcaggacgca aggttattcg
     7981 tgaagccaac aaaccttatt caggaacggc tgttatcgat gattttggac cacgtcagat
     8041 ggaaactgga gagttgatta tctatacttc agctgaccct gttttgcaga ttgctgccca
     8101 cgaagacatt attcctttgg atgaattgta ccgtatctgt gaatacgctc gttcgattac
     8161 ccttgagcgt cctgcccttc ttggtcgcat cattgctcgc ccttatgtag gtgaaccagg
     8221 taacttcact cgtacggcaa accgtcgtga cttggctgta tctccatttt ccccaactgt
     8281 tttggataaa ttgaatgagg ctgatatcga tacttatgct gtgggtaaaa tcaacgatat
     8341 ctttaacggt gctggtatca accatgacat gggtcacaac aagtcaaata gtcatggaat
     8401 tgatacacta ttgaagacta tgggacttgc tgagtttgaa aaaggattct cattcacaaa
     8461 cctagttgac tttgatgccc tttacggcca tcgtcgtaat gctcacggtt accgtgattg
     8521 cttgcatgag tttgatgaac gcttacctga aattatcgca gctatgagag agaatgacct
     8581 tctcttgatt actgcggacc atggaaatga cccaacgtat gcaggaacgg atcacactcg
     8641 ggaatatatt ccattgttgg cctatagccc tgcctttaaa ggaaatggtc tcattccagt
     8701 aggacatttt gcagatattt cagcgactgt tgccgataac tttggtgtgg aaactgctat
     8761 gattggggaa agtttcttag ataaattggt ataagatgac gcgctatgct ttgctggtga
     8821 gaggtatcaa tgttggtagt aagaataagg tcgtcatggc ggagcttcat caagaattga
     8881 caaacttggg actggaaaag gttgagagct acatcaacag tggcaatatt ttctttgctt
     8941 cgatagattc caaagcccaa ttggttgaaa agctagagac tttctttgca gtccattatc
     9001 catttattca gagcttttct ttactgagtc tagaggactt tgaggcggaa cttgaaaatc
     9061 taccagcttg gtggagcaga gacttggcac gaaaagattt tctcttttac actgagggtt
     9121 tggatgtgga ccaagtcatc gcgacagttg aaagtttaga gctgaaagat gaagtgcttt
     9181 attttggaaa acttgggatt ttctggggga aattttctga agaatcctat tctaagactg
     9241 cctatcagaa gtacttgctg aaggtgcctt tctaccgcca cattactatt cgtaatgcta
     9301 aaacctttga caaaattggt caaatgctaa aaaaataata aaggagacac acaatgacat
     9361 ttttaaacaa aatccatgaa actgctactt tcctgaaaga aaagggaatt gcagcccctg
     9421 agttcggtct aatccttggg tcaggacttg gagaattggc agaagaaatc gaaaatccag
     9481 ttgtagtaga ctatgctgag attccaaact ggggccgttc aacagtagtc ggtcatgctg
     9541 gtaaattggt atatggtgaa ctggcaggtc gcaaggtctt ggctcttcaa gggcgtttcc
     9601 atttctatga agggaatcct ctggaagtgg tgactttccc agttcgtgtg atgaaagttc
     9661 ttggatgtga aggtgttatt gtaaccaatg cagctggcgg tatcagattt ggtcctggta
     9721 ccttgatggc tatctcagac catatcaaca tgacggggca aaatccattg atgggtgaaa
     9781 acttggatga ctttggccca cgtttcccag atatgtctag ggcctacaca ccagaatacc
     9841 gtgccactgc ccatgaagtg gctaaaaaac ttaatatcaa gattgatgaa ggtgtctata
     9901 tcggagttac tggtccgact tatgaaacac cagcagaaat tcgttcctat aagacactgg
     9961 gagcagatgc agttggtatg tctacggttc ctgaagttat cgtggcagcc cactctggct
    10021 tgaaagttct gggaatttca tgtatcacta actttgcggc tggtttccaa gaagaactca
    10081 atcacgaaga agttgtagaa gtgactgaac gtgttaaagg tgatttcaaa ggcttgctta
    10141 aagcgattct tgctgaattg taagaaaaaa gatttaaaag ggggagtgcc tctgtctttt
    10201 caggattgac tgcctatccg tattaaagaa gaaacagagg aatactatga gcttcttcct
    10261 gctcttataa ctgaaagaag cggaagaata ggtgtgtctg atctgatagc cagcattgtg
    10321 aaagacaaga ttctaggata ctagcattag cttcctagcc aagcagacta gtatgataag
    10381 gagagatgag aatgaattga ctttctgaat ttctcagtct tatcatatat agcacaatga
    10441 gatttcgctt gagtctgctt gtaaataaac gaaaagaaag ataagaaata atgaaaattg
    10501 gtcaacgaat tatgcgcttt ggcataaaaa attaagtatc ggagttgtat ctgttgtagt
    10561 cggctttgat ttctagctcc agctggaatt tcagccaatg aagtaaagca agatgtaaca
    10621 tctgaagtgg taataggtgt gctagattct aaggaggaat tgaaagagtc agaaaatgat
    10681 gctccaaaac tagaaactcc tcttagagag gagccaagac tagctcctca aacgcttccg
    10741 gaagcaagtg aagttcttga aaacaaaagg gaagagtcaa aagtagagat aacagagcca
    10801 gctcaagcgg atgatatccg caaggttgtt ggggaattag ccaaggatat aagtattact
    10861 aagttgtata tgacaggtca ttctcttgga ggctacctag ctcagattgc agcggttgaa
    10921 gattaccaaa aatatcctga tttttataac catgtattga ggaaagtgac aactttcagt
    10981 gctcctaaag tgattacttc cagaactgtt tgggatgcta agaatggttt ctgagatgtt
    11041 ggtttggaaa gtcgtaaatt agctgttagt ggaaaaatta agcattatgt ggttgataat
    11101 gacaatgttg tgactccctt gattcataat aatcgtgata ttgttacatt tacaggtaat
    11161 tcacgcttta aacaccgttc tcgtggctat tttgaaagtc caatgaatga tattcctaac
    11221 tttaatattg gtaaacaagc taccttggat aaacatggtt atcgtgatcc gaaattggat
    11281 aaagcgcgat tctttaagaa acaggctcta cctcaatctt ctagtcaacc aagcgctgaa
    11341 ccaatggaaa atattgcctt aggaaaacag gttactcaaa gttcgacagc tttcggagga
    11401 gatgctagaa gagctgtgga tggcaaagtc gatggtaact atggtcacaa ttctgtcact
    11461 catacaaact tccaatctaa gccttggtgg caagtagatt tggctaaaga agaaaccatt
    11521 cgccaaatca atatttacaa ccgaacagac actgcccagg atagattggc aaactttgat
    11581 gtcattcttt tagacagttc tggtaaagaa attgagtgaa aacgtataac atctcctaaa
    11641 gatgtgtcag cacaaattac gattaaccat aaaaaagtgc gctatgttcg gattgagcta
    11701 gaaggctata atgccctcag tcttgcagaa gttgaagttt tccgctttat agctacgaat
    11761 gctgaaacgg cgacacaagt ttctaagcca gttcaaccaa tcagtcagac tcctgtgaaa
    11821 gataaaacat tgacaattca acatagtgga gcttacattg cccgctactc cataacttgg
    11881 gaagaagttc cagtagataa agatggaaac caagttgttc gtagtcattc ttgggaagga
    11941 aacggttgca accagactgc aggttttgtc ctcaacctcc caatcaaaga aaatatgaga
    12001 aatctgcgag ttaagattga gaaaaagacg ggcctactat ggaatagatg gcaaacaatc
    12061 tatgaaaaca gaccaatttt agctcaaccc caccgtaaaa ttacccattg gggtacgaca
    12121 ttgaattcca aggtgagtga cgatgatgtc ttgtaatctg atggtacaat gacagttagt
    12181 ttgtctagtt tataagaaag tactacctga gcttgaatag aactcaggta gctctctgtg
    12241 aaagaacaaa att
//
LOCUS       DBERMR010000061        12033 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.061, whole genome shotgun sequence.
ACCESSION   DBERMR010000061 DBERMR010000000
VERSION     DBERMR010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12033
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.061"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            91..550
                     /locus_tag="ACL8A1_001850"
                     /pseudo
     CDS             91..550
                     /locus_tag="ACL8A1_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            553..1564
                     /locus_tag="ACL8A1_001851"
                     /pseudo
     CDS             553..1564
                     /locus_tag="ACL8A1_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259617.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
     gene            1539..2117
                     /locus_tag="ACL8A1_001852"
     CDS             1539..2117
                     /locus_tag="ACL8A1_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron permease"
                     /protein_id="HGS1202563.1"
                     /translation="MVRNYWKAKSWLLVLALSFLILSPAGAQESLSFFFVKITDASKA
                     VKNGGQTETQKLVTKMASDFERVENKDSEVGKIVKEKLALSGDITEAKLTEISSALLA
                     FEKEQNPVDLDAEKEKLVNRLSPRFETLEQAIASKDLEKVREAFKKMNSTWTINESMV
                     RDNSTAHYGRVETAISFLPSSMEIEPTDESGT"
     gene            complement(2174..2416)
                     /locus_tag="ACL8A1_001853"
     CDS             complement(2174..2416)
                     /locus_tag="ACL8A1_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HGS1202564.1"
                     /translation="MKKDIHPEYRPVVFMDTTTGYQFLSGSTKRSNETVEFEGETYPL
                     IRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(2512..3447)
                     /locus_tag="ACL8A1_001854"
     CDS             complement(2512..3447)
                     /locus_tag="ACL8A1_001854"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HGS1202565.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQEVLKQYNVTDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            3551..3994
                     /locus_tag="ACL8A1_001855"
     CDS             3551..3994
                     /locus_tag="ACL8A1_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HGS1202566.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRIFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEDLAAKVG"
     gene            4110..4376
                     /locus_tag="ACL8A1_001856"
     CDS             4110..4376
                     /locus_tag="ACL8A1_001856"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HGS1202567.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEEVVAYKKASGKPILD
                     TKRETVIFEKIRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            4373..4747
                     /gene="crcB"
                     /locus_tag="ACL8A1_001857"
     CDS             4373..4747
                     /gene="crcB"
                     /locus_tag="ACL8A1_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HGS1202568.1"
                     /translation="MKKEQFYPLGIFIAAMLGGLIRYLVSIWLPASPDFPWGTLLVNY
                     LGIFCLIYLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDAVKLLDTGRYLSLI
                     LYLLLSIGGGLLLAYYLGRKKW"
     gene            4741..5070
                     /gene="crcB"
                     /locus_tag="ACL8A1_001858"
     CDS             4741..5070
                     /gene="crcB"
                     /locus_tag="ACL8A1_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HGS1202569.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            5189..5536
                     /gene="rplS"
                     /locus_tag="ACL8A1_001859"
     CDS             5189..5536
                     /gene="rplS"
                     /locus_tag="ACL8A1_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HGS1202570.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            5578..5649
                     /locus_tag="ACL8A1_001860"
     tRNA            5578..5649
                     /locus_tag="ACL8A1_001860"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5611..5613,aa:Arg,seq:cct)
     gene            complement(5704..6510)
                     /gene="yidA"
                     /locus_tag="ACL8A1_001861"
     CDS             complement(5704..6510)
                     /gene="yidA"
                     /locus_tag="ACL8A1_001861"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HGS1202571.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYERYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            complement(6523..7827)
                     /locus_tag="ACL8A1_001862"
     CDS             complement(6523..7827)
                     /locus_tag="ACL8A1_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HGS1202572.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSQRFVNRKVFKSITFSQEDQDQLASMRKLAE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            7899..8276
                     /locus_tag="ACL8A1_001863"
     CDS             7899..8276
                     /locus_tag="ACL8A1_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HGS1202573.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            8367..8699
                     /locus_tag="ACL8A1_001864"
     CDS             8367..8699
                     /locus_tag="ACL8A1_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HGS1202574.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            8711..10282
                     /gene="ffh"
                     /locus_tag="ACL8A1_001865"
     CDS             8711..10282
                     /gene="ffh"
                     /locus_tag="ACL8A1_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002902912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HGS1202575.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPNRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(10477..11760)
                     /locus_tag="ACL8A1_001866"
     CDS             complement(10477..11760)
                     /locus_tag="ACL8A1_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HGS1202576.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLEANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLIGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
BASE COUNT         3548 a         2218 c         2766 g         3501 t
ORIGIN      
        1 gaaacaagaa caggacaaat cgatcaggac agtcaaatcg atttctaata atattttaga
       61 agtagaggtg tactattcta gtttcaattt attatatttc gtctgatggg caaatcttat
      121 aaagagatta tagaactttt agactgtaat caaacaacga tttagcgaaa tgtaaaaaaa
      181 tatgaggagt tcggactcga ctctctcctt caagaaacac gtagtggtcg taaccatgca
      241 tatatgacag ttgaggaaga gaaagccttt cttgcccgtc atttgaaggc tgcagagtca
      301 ggagaatttg ttacaattga tgccttattt caggcttata aaaaggagtt aggtcgttcc
      361 tacacacgtg atgccttcta tcaactgttg aagcgccatg gttggcgaaa tattatgcca
      421 cgtccagaac atcctaagaa agcagacgct caaaccattg tcgcgtctaa aaataaaatc
      481 tcaattcaag aagacaagaa agcggtttaa atatagtaga cgttttcgta aggttcgctt
      541 gatgtaccaa atagtacgcc catgcaaaag aatatctatt ttgttgtctt ggacctgcat
      601 acgacggata gagataaaat tatccagcta ttcaaggatt ggacggatta tagtgccaag
      661 ttggtcgagg gggaattggt taaaaaagat ggacagaatg ctctctttcc tccgagcgat
      721 actggtgaga cagttgggct caatcctcat cgtttgacgc tgacttttgg tgtatctgca
      781 agtttcttga aaaagatgaa cttggaaaac aagcgccctc gactgttcag ggatttacct
      841 ctatttccta aggagcaatt acgtgaaaaa tatactggtg gagatattgt gattcatgct
      901 tgtgcagatg atgagcaaat tgcctttcac gttattcgca atctcatacg taaggggaga
      961 aatgcggtgc cccttcgttg gagtcagtct ggatttgcag ctatcgggga cgaatggaga
     1021 ctccgtggaa cctctttggt tttaaggatg gaacagcaaa tccaaccaag gagcaagact
     1081 ttgaccgcgt tatctgggct gatagtaagg actggatgga aaatggttct tatatggcag
     1141 ttcgtcggat tcagatgttt ttagagacct gggaccgcac tggtctcgaa gaacaagaaa
     1201 atacctttgg tcgttacaag gaaagtagtg cccctttcgg taaaaaaatg agtttgatga
     1261 agtgaatttg agttttttgc tagatgattc gcatgttcgt ttggctaagt aagtggacaa
     1321 gccacttttg cgttgttctt actcttactc agatggcatt gatgaaaaga ctggtcagtt
     1381 tgatacggga ttgctcttca tttctttcca gaaggatcct gataactttg ttaaggttca
     1441 aactaaccta ggtgctactg ataagatgaa tgaatacatc actcacatag gtagtggact
     1501 ttttacttgc tttggtggtg tggagaaagg aggctacatt ggtcagaaat tattggaagg
     1561 ctaaaagctg gttactagtt ttagctttgt catttttaat tctttcccca gcaggtgccc
     1621 aagaaagctt gagttttttc tttgtgaaaa tcacagatgc atccaaagct gttaaaaatg
     1681 gtggtcagac tgagacccag aaactcgtta cgaagatggc atcagacttc gaaagagtag
     1741 aaaataagga ttctgaggtt ggtaagattg ttaaggaaaa gttagctcta tctggagata
     1801 ttactgaagc caaactcacc gaaatttcct cagctctatt agcttttgaa aaagaacaaa
     1861 atcctgttga cctagatgcg gaaaaggaaa aattggtaaa ccgtctaagt cctcgttttg
     1921 aaaccttaga gcaggccatt gcctccaaag atttggaaaa ggttcgagag gcctttaaga
     1981 aaatgaattc cacttggacc atcaatgaaa gtatggttcg tgacaatagt acagctcatt
     2041 atggacgtgt tgaaacagct atttccttcc tgcctagtag catggaaatc gaacctacag
     2101 atgagtctgg aacatgaaaa cagcagttga tttcaagaaa caaaaaagaa cagccgaaac
     2161 tgtcctctta tcattatttg agaccgtatt ttttgttgaa acgatccacg cgtccatctg
     2221 cttgagtgaa cttttgacgt ccagtgtaga atgggtgtga gtctgatgaa atttccacac
     2281 ggatcaatgg gtaagtttcg ccttcgaact caactgtttc gttagagcgt tttgttgaac
     2341 cgctaaggaa ttggtaacca gtagttgtgt ccatgaagac aactgggcga tattctggat
     2401 ggatatcttt tttcattttg caatatttcc tttctgccat ggtctctttg cgagccatag
     2461 attgttacca tactagtcta tcagattctg aaaagtttgg caagtatttt atcagttttt
     2521 aagcaagttt tttaactttt ggtagatgat ttcattttct tctaggctat aggaattagc
     2581 accacttgct agagggtggc ctccaccatc atgctccttg gcgatttcat tgataggatg
     2641 gattttactg cgtaagcgaa ctcggtagtg gccatcagcc tgttcgacaa aaattcccca
     2701 gagactcact ctgtcaatgc gtccaggtgc accgacaatg gctgcagttt cagcatcggt
     2761 tacattgtat tgtttcaaga cttcttgact caggataacg cgagcagcac cattttcatc
     2821 cacttccaga tggtcgtaga tgtagccttg cagtttagca attttgtagc tcatagtgtc
     2881 cattttgcga gtgagagccg caaagtcaaa gttatgttct ctcaaataag cagccaggcg
     2941 aagagtccgt gcagtggtag aagggtagag gaagcgacct gtatcaccga caattcctgc
     3001 aaagagcaac tcagcatcgc gatctgccaa ggctagttgg gttgtttggg caaatagggt
     3061 aatcatctcg ctagcgctac ttgaactagt atcgacccaa gacaggtcac cgtatacatc
     3121 atcatttgga tggtggtcaa tcttaatgag aaaatcacct tgactatagc gcttatcatc
     3181 gatacgagca gtattagctg tatcacagac gatgacaagt gcgccttggt aggctctatc
     3241 ttcaacaaga tccatctcag ccatccaagt aagagttggt tcatcaaaac cgacggcttt
     3301 gatggttttt tctgggaaat gatgttccag caaggctttc aatcccacct gacttcccaa
     3361 ggcatcaggg tctggtttca tatgacgatg aatgataatg gtgtcgtatt ctttaatttt
     3421 ttctaaaatt tgttggcaaa tctccataaa taacctcaat tctttctcat ttcattgtag
     3481 cacaagaatt tttattttta aaatgaaaaa gatgtgatat aatggagaaa gataaattga
     3541 ggaggacgat atggcattag caaaaattgt atttgccagt atgaccggta ataccgaaga
     3601 aattgcagat attgtagcag acaaattacg tgacttgggc ttggatgttg atgttgatga
     3661 atgtacaact gttgacgctt cagacttctt ggaagcagac atcgctatcg ttgcgaccta
     3721 tacttatggt gatggtgaat tgccagatga gatgatggac ttctacgaag acctagcaga
     3781 tctcaacttg aatggcaaaa tctacggagt ggtcggctca ggagatacct tctacgatga
     3841 attctgtaag gctgttgatg actttgaccg tatctttgtg tcaacaggag cagaaaaagg
     3901 ttctgagtgt gtcaaagttg atctttctgc cgaggaagaa gatattgaac gcttggaaca
     3961 attcgcagaa gacttggctg ctaaagtagg ataagcataa aagtgcgctg atgtaatcaa
     4021 tcagtgcatt tttcttatcg agagttggga ttttactagc ctaattgatg gctttttgat
     4081 acaataattc aaataaagga ggagactgta tggatttaga tattattcgt caagaaatcg
     4141 atcaaatcga cgaccaaatt gtcaaactgt tggaagaacg aatgcactta gttgaagagg
     4201 ttgtggctta taagaaagct tcgggtaaac cgattttaga taccaagaga gaaacagtta
     4261 tttttgaaaa aattaggagt cgtgtagagg acaagcgcta tcaggagact attgtcgcaa
     4321 cgttttcgga tatactcaaa cgttcgcgtg attatcagga tcagaatatc aaatgaaaaa
     4381 agaacaattt tatccgctag gcatttttat agctgccatg ttgggcggac ttatccgcta
     4441 tctggtttcc atttggttac cagccagtcc agattttccc tggggaaccc ttcttgtcaa
     4501 ctatctaggc attttctgct tgatttatct tgttaagggc tatctggttt ataagggaac
     4561 tagtaagggc ttgattttag cactgggtac aggtttttgc ggaggtttaa caaccttttc
     4621 tagtctcatg cttgatgctg tgaaattact ggatacaggg cgttatctta gtttgatact
     4681 gtatttgctt ttgagtatcg gtggaggcct gcttttagct tactatttgg ggaggaagaa
     4741 atggtaatcg tctatcttgc aatcgcctgt ggcttgggag cactcgtacg gtatttcttt
     4801 tcccgctaca atcaagcttc taaattgcca ctcggaacgc tcatagctaa tcttttaggt
     4861 tgttttttaa ttggagtatt ctacaatcat gtagagtcta aggaagtata tgctattcta
     4921 gcaacaggat tttgtggcgg tttaacaact ttttcgacct tgaatgacga acttcaaaga
     4981 ctgctaagtg ataagaaggt cttttattct tacctgactt tgacttacat aggtggtttg
     5041 gttgcgattt ttttaggaat tttgctataa atttctttac ttttttgtga aaatttggta
     5101 gactgtatct tgtgtgtgat ggacgcacaa aaatacagtt tatccgctga ggaagtttcc
     5161 tcaagattga caaagatagg agaataaaat gaatccatta atccaaagct tgactgaagg
     5221 tcaacttcgt acagatatcc catcattccg tcctggtgac actgttcgtg tacatgcgaa
     5281 agttgtcgaa ggtaaccgtg aacgtatcca gatttttgaa ggtgttgtta tcgcacgtaa
     5341 aggtgctgga atctcagaaa actacacagt tcgtaaaatc tctaacggtg taggtgttga
     5401 gcgtatcttc ccaatccaca ctccacgtgt tgaaaaaatc gaagttgttc gttacggtaa
     5461 agtacgtcgt gcgaaattgt actacttgcg tgctcttcaa ggtaaagcag ctcgtatcaa
     5521 agaaatccgt cgttaattcg actaaaaaac tctgcttttc agcagagttt ttatcaagct
     5581 cccttagttc aatggatata acaactccct cctaaggagt agttgcaggt tcgattcctg
     5641 cagggggcag taattagaca tcaaaaaccg ctcaactgag cggttttagt ctattctttc
     5701 cttttacagt acccatgtac gaatagcatg ggcaacgccg gattcgtcat ttgttttggt
     5761 gatgtatttg gcgatttttt tgatttctgg atttccattt tccatgacaa cggggtttcc
     5821 aacgacttcc agcatggcac ggtcattttc ttcatcaccg attgccatgg tttcatcttt
     5881 ggtcaatccg agtttttcag ccaagtgagt aatggctgaa cccttgtcta cattcttttt
     5941 aaggagttcg aggtagaaag gagcagattt gttgatggag tagcgctcgt aaaattctgc
     6001 tggaattttt tcaatcgcag catcgagaat ttctggttca tcgataaaca tacatttaac
     6061 aatttctttg ccagccattt cttcaggggt acggtagaag ataggcatgc tgacgagggt
     6121 tgattcgtgt acagtgtatt ttccgatatt gcgatttgca gtatagatac cgtccttggt
     6181 aatggcatgc atgtggacac cgagcttgcg actgaggaat tccatatcca gataatcctc
     6241 ataggtcaag gattcgctga taatctcatg gccagtagca gtttcttgga caagggcacc
     6301 attgaaggtc accacatagt caccttggtc tctcaactgc aagtcgtcca gaagtttggc
     6361 aacacctgcg atggggcggc cagttgcaat cacgactttg acaccagctt ctttggcatc
     6421 ttggatggca gaaaagactt caggagtgat ttccttttgg ctgttgacta gggttccgtc
     6481 aatatcgacg gcaattagtt taatactcat aaatttcctc ttctagtttt tatttggggt
     6541 aaaatgatcg ttctcaatca agtgtaaaaa ttgctgggta atgcttgcga agatgctgtt
     6601 ttggtctaac atttctttag ggaaatagaa acgattatct ccgtggcgac tgccagcaag
     6661 ggattggacg ataggagaca ggctagagag ttcggccagt tctccatttt tttgtaaaat
     6721 ctcaatctgt gtccgtgggt tttcagattc gggacgatag atatcataag ggaggtcaaa
     6781 gttcttatga atggcagtgt agtagtcggg atcaaagccg atatcctcag ctaattttct
     6841 catgctggcg agttggtctt ggtcctcttg tgaaaaggta atggatttaa agaccttgcg
     6901 gttgacaaag cgttgtgaca agtctgcgag aatcttgtca ggactggtca tccagagttg
     6961 aaagtaggta ttcatcacac catcatctag agccagatag tcagtcaagg tcacattttt
     7021 ttcaaagaaa ggcaggaggt gtggagaagt tcgtgcaaag aagtccttgt cttcaggata
     7081 gagttccttg gcgcgtttga gaagattctg taggagaact tccatggcgc gtgttgcggg
     7141 gtggaaataa acctgcatgt acatctggta gcgactgagg acgtagtctt cgatggcgtg
     7201 catgccattg cgctgaaagg cgataccatt ttcgatagga cgaatgactc ggaggattcg
     7261 agtcaggtca aattccccat aggatgctcc tgtaaaatag gagtcacgca agagatagtc
     7321 catgcggtcc gcatcaatct ggctagaaat gagctgcacg acctgcttat taggataggt
     7381 atggtcaatg acactggcca ccttttctgg gaaatcaggt gccacttgta gcaggacttg
     7441 gtgaatctct gtctcaggat tttgaataat ctcctgagta atggcttcat ggtctgtatc
     7501 aaagagattt tcaaaagtat gggagtaggc accatgccca aggtcgtgta ggagagcagc
     7561 ggtcatggtc aagagagact cggcaggatt ccattcctca ggatattttt cttcgaaaat
     7621 ctctgtgatg cgtcgtgcaa tttcatagac tcctagacag tgagagaagc ggctgtgctc
     7681 tccaccgtgg aaggtataac tggaggttcc cagttgcttg atacggcgca aacgttgaaa
     7741 ttcttttgta ttaatcaagt catagatgat ttgattattg acatggatgt agttgtgaac
     7801 tgggtcacgg aatacttttt cgttcatatg accattatag caaaatcctg ctctttttgg
     7861 taaaatagta ggacaagaga caagaaagag ggcctgatgt gaagattcgg atgcgaaata
     7921 caattcagtt tgatgagcag ttggaagtga ttgaccagct ttatgatgtg gaagtgcatg
     7981 aaaaaggaga ttatagctac ctgcttttct ataatgagga aaaggaaaaa gtagtgatta
     8041 aatttcatgg tcaagaactt gtgatgagcc gattttctaa tcccaagacc attatgcgct
     8101 ttctaaagga tagcgatagt ttagcctata ttcctacacc tatgggcatg caggagttta
     8161 tcatccaaac gagccattat caagttgata ggcaaaagat tgaactagat tatcaactac
     8221 aaaatcaaga gggacatcct tttgccagct atcaattgga aattacttgg ggctaggctc
     8281 atgtcttttt caaatttagc tatctatctt cttggatcgg cttcctagtg tggtagatta
     8341 ggctaatttt tggtataata aaagttatgg aaatcgaaaa aaccaatcgt atgaatgcgc
     8401 tctttgaatt ttatgcggcg cttttgacag ataagcaaat gaattatatc gagctctact
     8461 acgctgatga ttacagcctt gctgaaattg ccgaggagtt tggtgtcagt cgtcaggctg
     8521 tctatgacaa tatcaagcga acagaaaaga ttctggaaga ttatgagatg aaattgcaca
     8581 tgtactcgga ctatattgtc cgcagtcaga tttttgatca gattttggag cgctatccca
     8641 aggatgactt tctgcaggag cagatagaaa ttttaacaag cattgataat agagaataag
     8701 aggaagaaaa atggcatttg aaagtttaac agaacgtttg cagaacgtct ttaaaaatct
     8761 acgtaaaaaa ggaaaaatct ctgaatctga tgtccaagag gcaaccaaag aaattcgctt
     8821 ggccttgctc gaggccgacg ttgccttgcc tgttgtaaag gactttatca agaaagttcg
     8881 tgagcgtgca gtcgggcatg aggtcattga tacacttaat cctgcgcaac agattattaa
     8941 aatcgttgat gaggaactga cagccgtttt aggttctgat acggcagaaa ttatcaagtc
     9001 acctaagatt ccaaccatca tcatgatggt tggtttacaa ggggctggta aaacaacctt
     9061 tgctggtaaa ttggccaaca aactcaagaa agaagaaaat gctcgtcctt tgatgattgc
     9121 ggcggatatt tatcgtccag ctgccattga ccagcttaag accttgggac aacagattga
     9181 tgtgcctgtc tttgcacttg gaacagaagt accagctgtt gagattgtac gtcaaggttt
     9241 ggagcaagcc caaactaatc ataacgacta tgtcttgatt gatactgcgg gtcgtttgca
     9301 gattgatgag ctcctcatga atgagcttcg tgatgtgaaa gcattggctc aaccaaatga
     9361 aatcttgctt gtcgttgatg ctatgattgg tcaggaagca gccaatgttg cgcgtgagtt
     9421 taatgctcag ttggaagtga ctggggtcat ccttaccaag attgatggcg atactcgtgg
     9481 tggtgcggct ctatcggttc gtcacatcac tggaaaacca atcaagttca ctggtacagg
     9541 tgaaaaaatt acagatatcg aaaccttcca cccagaccgt atgtctagcc gtatccttgg
     9601 catgggggat atgctcactt tgattgaaaa agcttctcag gaatacgatg aacaaaaagc
     9661 ccttgaaatg gctgagaaga tgcgcgaaaa cacctttgat tttaatgatt tcatcgatca
     9721 attagatcag gtgcaaaata tggggccgat ggaagacttg ctcaagatga ttccaggtat
     9781 ggccaacaat ccagcacttc aaaacatgaa ggtggatgaa cgccagattg ctcgtaaacg
     9841 tgccattgtg tcttcgatga cacctgaaga acgtgaaaac ccagatttgt taaatccaaa
     9901 ccgtcgccgt cgtattgctg ctggttctgg aaatacattc gtcgaagtca ataaattcat
     9961 caaggacttt aaccaggcta aacagctcat gcagggtgtt atgtctgggg atatgaataa
    10021 aatgatgaag caaatgggga ttaatccaaa taaccttcct aaaaatatgc caaatatggg
    10081 aggaatggat atgtctgccc ttgaaggaat gatgggacaa ggcggtatgc ctgacttatc
    10141 agctctcgga ggagcaggaa tgccagatat gagccagatg tttggtggcg gtttgaaagg
    10201 taaaattggt gaatttgcca tgaaacagtc catgaaacgt atggctaaca aaatgaagaa
    10261 agcgaagaag aaacgcaagt aagacaagag attgatgcaa atctcatttg atgagaaaat
    10321 aaagtaaaaa tttctatgat aaaacgtaca gtatcaaggt tttagaatat ctgatattat
    10381 acgttttttg attttaataa ttttgttaag acacttttat tatggtatac gaaaaatcca
    10441 ctcagctgtc tgagtggatt aggtgtattt agactcttag tctttatttt cgtatggcaa
    10501 gatcaagttc aagatgattc ctgtcatggc tgaaagggca gtacctgaaa gtgtaactgg
    10561 accaagttta aggatagctc ctccaagtcc aagaaccaac atagcacttg cgatgatgag
    10621 gtttcgcatt tgagcgaaat caacacgttc tttaatcaag actttcaaac cattgctggc
    10681 gataacccca tagagaagga ttgacatacc accaagtaca gcgtttggaa tagttgaaat
    10741 caaggcagtg aatttaccaa ggaagctgag ggcaatcgcg atgaaggcag cgttacggat
    10801 aactgagaca gaagcgatac gagtcatacc gataacccct gtattttctc cgtaagttgt
    10861 attggctggt ccaccaagga aggcagaaac agaagttgcg ataccgtcac caataagagt
    10921 acggtgaaga cctggttctt ttaagaattg acggccacag atttgaccca aaacagtatg
    10981 gtctccgata tgttcagaaa ttgttacgat agcgattggc aagatagcga tggcttctgg
    11041 accaaagtaa agattgtact ctttaaaggc accacctgtg ctaaatggca agtagaaacc
    11101 aggaatttca aaccagttag cttctaggac aggcgtaaag tcaaccaaac caagagttag
    11161 tgcgaagagg taaccaccga taatggcaaa gaggaatgga atgattcgta ggaagccttt
    11221 tccttttgta ttgataaagg cagcaattag gaaagtaaca acggctacca gagcattttt
    11281 ccaatttccg tctgctacaa gacctgcatt ggtaacagct gaacctgcaa gtccaagacc
    11341 gataacgatg atcataggac cgataatgat tggtggcaag agtttatcaa tccattttgt
    11401 tcctacaaat cggatgctgg tagcaacaag gacatagacc aaaccagtca agataacccc
    11461 tgtttgggca gcagatacat ccccccccat ttctttcata gccagtgaca tagctgtgat
    11521 aaaggcgaat gaagaaccta gataaactgg aactttaaaa ccagtagcaa tcatgtagat
    11581 gagtgttcca acacctgaag caaaaagggc aacagataca ggcattccca aaatcaatgg
    11641 taccaagatg gtcgcaccaa acatggcgaa aacgtgttgg aaactaagga gaattccttt
    11701 tccagccgaa ggacgttggt caacgtctag taacaaatca acagttgatt cttgtttcat
    11761 ataaacctca cttttgggca tcaaaaaaga gcccattatt ttacagcaat aggccctcag
    11821 agtaagactt ctttaaaatt tagattttaa agagttttaa atatttctgc gtcttcgtca
    11881 cctcacggga tgacattaaa aacctttgct taggatagta tagcagaaaa aaatgatttt
    11941 gtaaatcatt ttttcccgag cctagaaata aaagagcgag gctgattttg taaatcattt
    12001 tttcccgagc ctagaaataa aagagcgagg ctg
//
LOCUS       DBERMR010000062        11995 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.062, whole genome shotgun sequence.
ACCESSION   DBERMR010000062 DBERMR010000000
VERSION     DBERMR010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11995
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.062"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            159..2432
                     /locus_tag="ACL8A1_001867"
     CDS             159..2432
                     /locus_tag="ACL8A1_001867"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HGS1202577.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQCQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
     gene            2449..2919
                     /locus_tag="ACL8A1_001868"
     CDS             2449..2919
                     /locus_tag="ACL8A1_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HGS1202578.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(3036..3191)
                     /locus_tag="ACL8A1_001869"
     CDS             complement(3036..3191)
                     /locus_tag="ACL8A1_001869"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202579.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDETDEVSNHTYGKATLTRIEEI
                     FEEYKSS"
     gene            complement(3462..6812)
                     /locus_tag="ACL8A1_001870"
     CDS             complement(3462..6812)
                     /locus_tag="ACL8A1_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HGS1202580.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIDQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDDSCEKDIDGSVFNSFIFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSPLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRSIVQIIELINNRAGEAA"
     gene            complement(6842..7417)
                     /locus_tag="ACL8A1_001871"
     CDS             complement(6842..7417)
                     /locus_tag="ACL8A1_001871"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGS1202581.1"
                     /translation="MKKVKLGQVATFINGYAFKPQDWSSEGKEIIRIQNLTKTSKGIN
                     YYSGTIDKKYIVEAGDILISWSGTLGVFQWCGRSAVLNQHIFKVVFDKIDIDKSYFKY
                     VVEKGLQDAVKHTHGSTMKHLTKKYFDNIIVPYTNLGEQQRIASELDLLSKLILRRQE
                     QLEELNLLVKSQFACEIAIKVWRNSLKFSII"
     gene            complement(7451..8416)
                     /gene="xerA"
                     /locus_tag="ACL8A1_001872"
     CDS             complement(7451..8416)
                     /gene="xerA"
                     /locus_tag="ACL8A1_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
                     /protein_id="HGS1202582.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKAQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            complement(8534..8947)
                     /locus_tag="ACL8A1_001873"
     CDS             complement(8534..8947)
                     /locus_tag="ACL8A1_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HGS1202583.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            complement(8944..9180)
                     /locus_tag="ACL8A1_001874"
     CDS             complement(8944..9180)
                     /locus_tag="ACL8A1_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HGS1202584.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLQSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            complement(9240..10361)
                     /locus_tag="ACL8A1_001875"
     CDS             complement(9240..10361)
                     /locus_tag="ACL8A1_001875"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGS1202585.1"
                     /translation="MKKVKLGEILSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLRDHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKRLITKRK
                     FQLDELNLLVKSRFNEMFGENKIFESIDNLFDIIDGDRGKNYPKSDELFSEEYCLFLN
                     TKNVTKNGFSFDTKQFITKTKDKLLRKGKLERYDIVLTTRGTVGNVAYYDELIKYKHL
                     RINSGMVILRPKTPNLNQKFIIHVLRNNNYSRVISGSAQPQLPITKLKKILLPLPPLA
                     LQNEFADFVVQVDKSQLAIQKSLEELETLKKSLMQEYFG"
     gene            complement(10374..11867)
                     /locus_tag="ACL8A1_001876"
     CDS             complement(10374..11867)
                     /locus_tag="ACL8A1_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGS1202586.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
BASE COUNT         3646 a         2478 c         1908 g         3963 t
ORIGIN      
        1 tataccctat ctatttattc ttattgttac tctgttctct atattgaatt tagtacttta
       61 ttcattttta ttggattatt tccttttgaa gttaacaatt ccctttctgc tttttttttg
      121 atataataac cttaactgtc attctatatg gaggttctat gcgttttaat caatatagtt
      181 atatcaattt tccaaaagaa aatgtcttat ctgaactaaa aaaatgtggc tttgatttac
      241 aaaatacagc caatcacaaa gactcacttg aaaccttttt acgtcgtttc tttttcactt
      301 atcaagacac caactaccca ctttctatcc tagcagctga taaaaaaaca gacctgctga
      361 cattttttca atcagaagat gaactgacag cagatatttt ttatactgtt gcttttcaac
      421 ttttaggctt ttcttatttg gttgacttcg aagatagtga cgtttttcgt aaagaaactg
      481 gatttcccat tatatatggt gacttgattg aaaatctcta tcagttactc aatactcgca
      541 ccaaaaaagg aaatactctt atcgaccaac ttgttagtga tggtcttatt ccggaggata
      601 atgactacca ctactttaac ggcaagagtt tggctacttt ttctaaccag gatgtcattc
      661 gtgaagtcgt ttacgttgag tctcgtgtcg atactgacca aaaagggcta tcagacctag
      721 taaaggttag cattattcgc cctcgttttg atggaaaaat ccctgccatc atgacagcca
      781 gcccttatca tcagggaacc aatgacaagg ctagtgacaa ggctctctac aagatggagg
      841 gcgagcttga ggttaagctt cctcacaaga ttgagctaga gaaacctcaa ctaaatctcg
      901 tccaacccca aggtaaagct gagcttatag cagaggctga ggaaaagcta actcacatca
      961 actctagcta tacactaaac gactacttcc ttccgagagg ctttgccaat ctctatgtct
     1021 caggtgttgg taccaaagac tcgacaggtt tcatgactaa tggagactac cagcaaatcg
     1081 aggcttataa aaatgtcatc gattggctta acggccgttg ccgtgccttt actgatcaca
     1141 cgcgccagtg tcaagtcaag gctgattggt caaacggaaa agttgctaca acaggacttt
     1201 cctatctagg taccatgtcc aatggtcttg cgactacagg agtcgatggt ttagaagtta
     1261 tcattgccga ggcaggaatt tcatcatggt acaactacta ccgtgaaaac ggtctggtga
     1321 ctagcccagg tggttatcca ggtgaggact ttgactccct tgctgagtta acctattctc
     1381 gtaatctctt agctggcgac tatatccgtg gcaatgaagc tcaccaagct gacttagaaa
     1441 aagtgaaagc tcagctagac cgaaaaactg gggactacaa tcagttttgg catgaccgca
     1501 attatctgct caatgcccat aaagtaaagg cagaggttgt ctttactcat ggttctcagg
     1561 attggaatgt caaaccactt catgtttacc agatgttcca tgctcttcct actcatatac
     1621 acaagcatct ctttttccat aatggtgctc atgtttacat gaacaattgg caatcaattg
     1681 acttccgtga gtccataaat gccttattga ccaagaaatt actaggacag gaaacagact
     1741 ttcaacttcc tactgttatc tggcaggaca atacagctcc acagacttgg ttatcacttg
     1801 ataacttcgg tgggcaagaa aactgtgaaa ccttctcact tggtcaagaa gagcaagcta
     1861 ttcaaaacca gtacccagat aaggattttg agcgctatgg taagacatac caaaccttca
     1921 atacagagct ctatcaaggg aaagccaatc agattactat taaccttcct gtgactaaag
     1981 atctccacct aaacggtcgc gctcagctca atcttcgtat caaatccagt acaaacaagg
     2041 ggctcttatc tgcccaactg ctagaatttg ggcaaaagaa atacctacaa ccttatccag
     2101 ctattttaag tgctagaacc attgataacg gtcgctatca catgttggaa aatctctgtg
     2161 aattgccatt tagaccagag gcacaacgag tcgtgacaaa aggttacctt aatttacaaa
     2221 atagaaatga tttactgtta gtagaggata ttactgcaga tgaatggatg gacgttcaat
     2281 ttgaactgca gccaactatt tacaagctaa aagaaggaga cactctccgt ttagtcctct
     2341 atactactga ctttgaaatc accatccgtg acaatacaga ctaccacttg actgtcgacc
     2401 tcgctcagtc catgcttacc ttaccttgct aaaaggagtt acattattat gaataaatca
     2461 gaacaccgcc accaacttat acgcgctctt atcacaaaaa acaagattca tacacaggct
     2521 gagttgcaag cccttcttgc tgagaacgac attcaagtaa cccaggcaac cctctcacgc
     2581 gacatcaaaa atatgaacct atcaaaagtc cgcgaagaag atagcgctta ttatgttctt
     2641 aacaatggtt ccatctcaaa atgggaaaaa cgtctcgaac tctacatgga agacgccctt
     2701 gtctggatgc gcccagttca acaccaagtc ctactaaaaa cccttcctgg actggctcaa
     2761 tcctttggtt ctatcattga tactttgagc ttccctgacg ctatcgctac cctttgtggt
     2821 aatgatgtct gtcttatcat ctgtgaagat gcagatactg ctcaaaagtg ctttgaagaa
     2881 ctgaaaaaat tcgccccacc atttttcttt gaagaataat aaatgactac aactaacaat
     2941 tatgcttaaa aaaaaactgg tagaaagtct ttaacatcag aaaacgcata gtatcaagtg
     3001 ttgaaaaccc ttgatactat gcgttttgct ttatcctagg aagatttata ctcttcgaaa
     3061 atctcttcaa tccgcgtcaa cgtcgccttg ccgtaggtat ggttactgac ttcgtcagtt
     3121 tcatctacaa cctcaaaaca gtgttttgag cagcctgcag ctagtttcct agtttgctct
     3181 ttgattttca ttgagtatta tttcattttc tcctgcaatt gaattcttgc tcagcttttt
     3241 gttttctatt tctttaaaat caaagtagct cttttgttaa taactcgatc aacaaacatc
     3301 gtggtacaag tatctacttt gaaatttatc aaccacttaa caactgatac tgtattctag
     3361 gaaaacgatg acattcttcc taataaaact tctcatatat agcataaatt tctactcttt
     3421 ttaattttaa atgagtctca gcatggcaat cactttagaa tttaagccgc ttctccagct
     3481 cgattattga taagttcaat gatttgaaca atactacgaa gtactggtag ttggtgttgg
     3541 aagagtagtt gaacagatcc ataagattta aacggctctt gcgttaacac tttcgtctct
     3601 aaaaaaccat tttctacaat gtagtctaca atcaatttta caaatgaaat ctgcctggca
     3661 ttaagattct catccgacaa aaatttagaa aaaatacgat tggcagactc tctatctaag
     3721 ccaataatct cacgaaccaa tctcggaatt gccttatttt cataatgact ttggtagtct
     3781 gctttactac ctaatttttc ccaaagcaat ttttcaagtg caagcatatc atcagatgtc
     3841 aactttttat tatggtatag cttagaaatg gactcctcat ccagatgagt tttcaaataa
     3901 tgctcaactt tttcattgta agaccgaaga tcgttgacct gcaaaaatgc tgtggtctcg
     3961 tgaacagtgg agagtatacg atcttcaaag ttaacatagt agggtttacg gtctgtttta
     4021 tccaaaaact gtaataaatc tcgaatagca agacgaattt tttccaaatc agacaagtta
     4081 acttctttcc aaaattcagg ctcctgtact ttcctgataa tttcagcctg ctcaaaaacc
     4141 tgcgggatat tgccaatagc agaaagagct ctagccgtct tcatcacttg ggaaatatga
     4201 acagtggaag atttagctgt cagttgcccc aactgaatat gaagcaacca caaatcaaat
     4261 ctcctcgcca tctcatcttc tttatcttca tcaaataaaa gcggagagag atttttttga
     4321 atggtttcac ttgtaacagc agttagattc tgccaacttt ccaatttcct atagctataa
     4381 actgtatcta aaaccatacg aaccctgaag tccaactcat ttaagctctc tatacgacct
     4441 tgaagttccg agacaagctg ctgacggtat gctctcgcaa actgatcttc ttggtattgg
     4501 agtccctgaa gttctcgaat caagtccact ttgatattaa ataaacgctg agtcagcgaa
     4561 acaatgtgac gaccctctcc atctcttgga tctgcacgaa aataatcaaa attgtcccca
     4621 taatcaaata ccaagaagtt ttccttatcc tgctcaggtc caaataaatc tttacataga
     4681 cgggttcctc gaccaatcat ctgccaaaac ttagttttag agcgtacttt cttgaagaag
     4741 actaaattaa caacctctgg tacatcaata cctgtatcta acatatcgac agaaatcgca
     4801 atttgaggat acttctcctt aattttaaaa tcatcaatca aggtctgata atgcttaata
     4861 ctataatcaa tcacctgagc atagtcgctc cctttttcag gatagcggtt gttaaaaata
     4921 cctctgatat attccgcatg atcatgattt ttagcaaaaa taatagtttt accaatttca
     4981 tcacccgagg ctgtctgaat tcctcttgtc atgagttcat ttaaaacaat ttctactgta
     5041 cttttattga aaataaagga attaaataca ctcccatcaa tatctttttc acagctatcg
     5101 tcttcaaatt tgctatcaaa atgttccttt tcttcttcgg acaaatcatc ataatgtaga
     5161 ccatccgtag gtagtttcag tttggtttcg atagaatgat aggctactaa atatccgtct
     5221 ttaacagcct cttccaaatc atatgcatat gttggaaccc cattctccaa attaaagaat
     5281 ccataggtgt ttttatctaa atcttgacgc ggagtagctg ttaagcctac aattcttgca
     5341 tcaaaataat caaaaatgga cttgtatttc tgataaatag aacggtgaga ttcgtcaatt
     5401 atgataaggt caaaatgccc aacagtgaaa gggcgttgat ttacttcttc ttgaccacta
     5461 atcgctccaa tcatggtcgg ataagttgaa aagaccatgc gacttgattg agctccttct
     5521 ttatcttcta agaagttaca aacggaaaga tctgggagta attttctaaa cgaatcatag
     5581 gcttgcttaa ccaaggaagt tctatcggct aagaagagaa cgttttttac ccagttatga
     5641 cgtgataaga tatcaactag agaaactgct gtacgagttt tcccagcccc agttgccata
     5701 accaaaagtg cctgtctacg atgattagag aaagcttcac aaacgcttgc aatggcgtgt
     5761 ttttggtagt gacgcccgga aatatcgtcc ctaattttac tagaaatatc ctcaagtggt
     5821 ttttgaagat gacgtctatc catcactaat tgcaattctt cttgagagta aaagcctgca
     5881 atctggcggc ggttcggacc atcaagtata tagtgcttca agccatttgt aataaagcaa
     5941 attggctgat agccgatatg tttctcaaga gcttcagcat attctttgac ctgtacttcc
     6001 cctacttctg gattgacaga ggctttttta gcctctacaa tcgctaaaat tttcccattt
     6061 ttaccataaa gtacatagtc acagtaacca attcctgaac cgtgcttgag accatcaacg
     6121 gctatctcaa cacgacagtt ttcttcaaat atccatcctg ctaaacggag atcgatatca
     6181 ataaagagct ttcttgtctc tgcctcagat aattgatcaa tatggaaagg cacatcttgc
     6241 cgttgcgctg cagtctcacg tttttcagta taagtctctt gaacttctac ggactgagca
     6301 gccatctgtt catgaaaatc aggcagttct ggtaaactgt cttgtaactt tatcatagat
     6361 tgtggagttt ctcggtattt gatgtttgct gaaagtggta agcacttctc atcaaaataa
     6421 cgctccacaa actcattgct gtaacaataa tcgataaaat taacaaactg atacaaatga
     6481 tgcaaagcta aaatcgcttc tcgttcctta atttcaccac catgagcagc atggtttccc
     6541 caccgaatca acagaactat ctgcttgtgg agttcagaat ctacgatatc cctaaaatca
     6601 tcatcccata ctaaagaaga tagagtagca cgatagggag cttctaaata tgaatcgtga
     6661 ctatatatcc aatggacagc ctgctctaaa gcacgacgcg ccataaaggc agtagccaca
     6721 gttgatgtag caatcatatt ctcagcttca atgcaaggtt ttgaaaaaga ttcatattcg
     6781 tcatttttta aaagaaagct aaaatttccc atttttttct cactttaaac aaatagttta
     6841 gctatattat actaaatttc aagctatttc tccacacttt tatagctatc tcacaagcaa
     6901 attgtgattt tacaagtaga ttaagctctt caagttgttc ttgtctcctt aaaatcagct
     6961 tgcttaacaa atccagctca cttgcaattc tttgttgctc tcccaaattt gtatatggaa
     7021 ctataatatt atcaaaatat tttttagtta aatgtttcat tgtggaacca tgagtatgtt
     7081 ttacagcatc ttgtaaacct ttttcaacaa catatttaaa atacgattta tctatatcta
     7141 tcttatcaaa aaccacttta aaaatgtgct gatttaacac tgcggatctt ccacaccact
     7201 gaaaaacccc taatgtaccc gaccacgaaa taagaatatc accagcttca acaatgtatt
     7261 ttttatcaat cgtacccgaa taataattta tcccttttga agttttagtc agattctgta
     7321 ttctgataat ctctttccct tcagatgacc aatcttgagg tttaaaagca tagccattga
     7381 taaaagtagc cacttgccct aatttcactt tcttcatgca accacctcct cggaatatcg
     7441 ggatttgctt ttaagagatg aatttttggt gtgaatgctt gacattattt tgattgacca
     7501 tggcataggc cagggttgtg tcaatcttgc tatgacctag cagtttttgg acttgttcga
     7561 taggcatacc tttatcaatt gccttagtcg ctaaagttct tctgaactta tgtgggtgaa
     7621 ccttttgtat gccgagtttg tctcctaact ttcttaagcg aatctctaca ccagcaattc
     7681 caagtctctg ggcttttcca actagcgtta caaaaagagc agggtgacta tcttttctgt
     7741 cgttaagata atttcttaaa tgaattttcg tccgagcatc aaaatatact ggtctctcct
     7801 tctttccttt accaaagaca acacactctc tgttttcaaa atcaatatct gaacgattca
     7861 actgtacaag ctcccctaca cgcatacctg tcgatgctag taggtctatt attgccaaat
     7921 ctctcaaatt ttcacagtta tcacgcataa tttccaaatg ttcatcagta taagtttcct
     7981 tcacattttg ctcagtctta attttcttta tccgtcgaat gggattttta atgatatatt
     8041 cctcttgctc aagccaagca aaaaaagaag acaatatacg cctaatattg tctaaatttg
     8101 ccttactaca atccttttca gactggtaat ttgctaaata actccttaaa tcatctgttg
     8161 tgagttgtgt cacagactct ttaatagcat taaacaagtt ttcaatcgtc ctctggtaat
     8221 aacgaattgt ttttgagcta cagccctcta cctgcttggc ggtaataaat aattgcaaca
     8281 ggttattatt cgaatacttc tgctgctttt ttccgtctac tagatgatgt tctaaactct
     8341 ctctcaacgc tagtaattgt tcattattta aaagaggtaa catttcttgc atgataattg
     8401 taacttttga ttccataatt tctcactttc ttattcatca ataaaaagag acaatatcag
     8461 tttctgcatt acagaattct aatattgtct gttcatttat tttcttgtgc tcattcaaat
     8521 ccagcatact aggttacttt ttgacctttt ctctaacaga atgttcagaa acccatttgg
     8581 aatagcttgt cattttttca tcagttaaag cttctactgc gatggttaca cacattttta
     8641 ctgcttcttc taccgttaca gaaaaacgat agccgtttaa ttgtaaaaat ttgaccaaaa
     8701 cgaagaaagc agttctttta ttagcattag caaaaacatg cttctttatc aattggacaa
     8761 atagtatcgt tgctttatca aaaattgttg gataaagaga cttcccaaag acaaattgtt
     8821 ctggtaagtt cacaatcata tttaaggcag aagaactaac tgtttgaatt ttctcatttg
     8881 gagaataccg ttgaattgct aaagcattta ttttttcaat ttgcttttct gtcagataga
     8941 ttgtcatttt tccaccaaag ctttgaaaac atcgtcgtat tcgtcaaata ttttatctaa
     9001 atttaagtca aaggcatcat cagatacata agaaacctgc tgccccaatt cttcaggagt
     9061 aaagataatc tcaccacttt gtaacaattt cgcttcgtat ttaacaccac ttggaatatt
     9121 aaattcactt ggaatcgtaa tggtgattga atttccttgt tttcttgttt ttactaccat
     9181 ctgatttcct cctttgaagt attataacaa atcattaccg taattacaat ggcagaatat
     9241 cagccaaaat actcctgcat cagagatttc ttcaaagttt caagttcttc cagagatttt
     9301 tggattgcca attgtgattt gtcgacctga actacaaagt ctgcgaactc attttggagg
     9361 gctagtgggg ggagggggag aagtattttt tttaattttg taattggtaa ctgaggctga
     9421 gcacttcctg atatcactcg actataatta ttatttctta aaacatggat aataaatttc
     9481 tgatttagat ttggtgtctt gggacgtaat attaccatac ctgaatttat acgtaaatgt
     9541 ttatatttta ttaattcatc gtagtacgct acatttccaa cagtacctct tgttgtcaag
     9601 actatatcat aacgctcaag tttgcctttt cgaagtaatt tatcctttgt tttagtgata
     9661 aattgctttg tatcgaatga aaatccgttt ttagtaacat tctttgtatt taaaaataaa
     9721 cagtactcct cactaaacaa ctcatctgat ttaggataat ttttgcccct atcaccatct
     9781 ataatatcaa ataagttatc aatgctttca aatattttat tttccccaaa catctcgtta
     9841 aatcgggatt tgacgagcaa gtttagttca tctaactgaa attttctttt agtaataagc
     9901 cttttaatcg tattaagaat acagataatg ttctcttgtt cttcgatacc tagcaattct
     9961 aattgtaaat caagtaatat attcttgttt aaatgaggaa tagttgcacc tgttgaatga
    10021 tctcgtaaat actgcgattt actttccaaa aagactccca agtaatctga tataattttt
    10081 tctttgtatc gctcattctt ttttaagacc gtaattgtac taccaacagc tcccgataac
    10141 ccatagccaa ctgttcctgc attagcacca tcccatgcta ttagaatatc atctgggagt
    10201 gcttcagtca tatttaaact ttcagtgaat tttaaattat tattatttct taaatcatct
    10261 atttgaatat aacgttggct tagagttgtt tgttcagcaa gtacagtggc tttctttcct
    10321 ttttttagag ataagatttc ccccaacttc acttttttca tacagccacc tccctacttg
    10381 agtaattttt ccaattcagc caagccagct tgaatttctt tttctaaatc attgattttc
    10441 tttaagatga cttctgttgg ttcatactca actttttcat actcaatctc tttatattta
    10501 ttgatagaca aatcataatc attttccttt atctcagcaa ctggaacaaa gaaagattga
    10561 tccgttctct gacgttctgc ttctttttca agatgatgaa agcgttcgat aatatctgga
    10621 atatcattgt cgctaatcgg ttgtcgctta tcatccaaac ttaaaccatc cgctttcata
    10681 tcgtaaaacc agactttgtc agtaccacca ttaccagttt ttgtaaagat gagaatggca
    10741 gttgaaactc cagcataagg cttgaacaca ccactaggca ttgagattac agcatcaagc
    10801 ttatgattct ctacaatttc ctgacgaatt cctttatgag ctttagacga accaaaaagg
    10861 acaccatcag gtacgataac tgctgctcgt ccacctggtt ttaaagttcg caagaaaaga
    10921 gaaagaaaga gtaattctgt ttttttggtt tttacggttg caagaaggtc attagaggtt
    10981 gaattgtagt caagtgagcc cttaaaagga ggatttgcta aaaccaaagt atatttatcg
    11041 gcttcttcat tatcttgaga cagcgagtca aggtaactga tttgtggatt ttctactcca
    11101 tgtagcatca tgttcatcgc cccaagtctc aacatagtcg tatccgtatc atttccatga
    11161 aacatctgat tatgaaaatg attgatatta tctgtattgg tttcccattc atctttctta
    11221 cgctttaagt aacggctagc agatactaag aagccagcag aacccatagc gggatctgag
    11281 atgatatctt tgatagtcgg ttgcatcaac tcaaccatca tatcgatgat gtgacgaggt
    11341 gtacggaact gtccattttt acctgcggtc gacaattttg ataacagata ttcatagata
    11401 tctccgatat cagtaatact ttgtttgtca ttatcaaaat ctacatctaa tcccctagtt
    11461 ggaaaaacat ctaagataga aattgccttt tgaagcgtag caggtttatt tatttgaaaa
    11521 atagcttctc gcatatatcg tgaaaaggct gtatcatctg tatccccctt gagattttta
    11581 ataaacggaa aaatctcctg agtcattaaa cgataaactt cctgagcatc tcctatattt
    11641 ttaaaagttg accaacggta ttcaggttta tcttttggaa aaactccctc ataaggaatc
    11701 cctagaaatt cagcatcact ttcacgtcca agctcgacac tatccaaatc tttcataaat
    11761 aagagataag tcaactgttc aatatttgtt aaaggatttg cgtttccttc tgtccaaaga
    11821 atttcccaca gctgatcgat tttatttttt aattcgcctg taatcatatc ttcccttttc
    11881 tttagttttt aaaacttagt aatatcatta ttttatcaga tttcaaacaa agcttcaatt
    11941 aactatatct atgcgtcacc tgaatatttt atactcttcg aaaatctctt caaac
//
LOCUS       DBERMR010000063        11993 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.063, whole genome shotgun sequence.
ACCESSION   DBERMR010000063 DBERMR010000000
VERSION     DBERMR010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11993
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.063"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(183..1814)
                     /locus_tag="ACL8A1_001877"
     CDS             complement(183..1814)
                     /locus_tag="ACL8A1_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HGS1202587.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            2103..7043
                     /locus_tag="ACL8A1_001878"
     CDS             2103..7043
                     /locus_tag="ACL8A1_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HGS1202588.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHTRENKLEKAEGVATASETAEAASAAKPEEK
                     ASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEKEVQPDVPKNTEKT
                     LKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNEKASKEAKVQALSN
                     TNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDAGHRNGVPVYGTLF
                     FNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFINQETTGDLVKPLG
                     EKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYNYQFMQPEGDKVPA
                     DNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKTKVKWNDILDENGK
                     LRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGDKDWYGIANLVADR
                     TPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWRWWQTSTGEKLRAE
                     YDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVAHKGGKGSKVYMAF
                     STTPDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFFEHEGAVKDYQFNL
                     GQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADFYEVYEKDGDSWKL
                     LTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTTFDWGMTVKDTSLP
                     KPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSAQLSGSVDIRLTKP
                     RTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEVRGNKAHVTDITLD
                     KPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMAKAAARSLGNNKVQ
                     VGFADVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQSGLLYYRTQLPGK
                     EISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLRVQYEGGTEDELIR
                     LTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEASPKTILGIEVSQEP
                     KKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQKTGRQTLTLHYQGH
                     EVSFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASSEVKEAFLKAIEAAEQVLK
                     DHETSTQDQVNDRLNKLTEAHKALNGQEKFTEEKTELDRLTGEVQELLAAKPNHPSGS
                     ALAPLLEKNKALVEKVDLSPEELTTAKQSLKDLVALLKEDKPAVFSDSKTGVEVHFSN
                     KEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVDLSYASIVKIPIEK
                     DKKVKKVFFLPEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESAEKPQPAKPAPQNT
                     VLPKPTYQPTSDQQKAPKLEVQEEKVAFHRQEHENTEMLVGEQRVIIQGRDGLLRHVF
                     EVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQNTAVKSEEASKQL
                     PNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            7133..8329
                     /gene="pgk"
                     /locus_tag="ACL8A1_001879"
     CDS             7133..8329
                     /gene="pgk"
                     /locus_tag="ACL8A1_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HGS1202589.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            8465..8992
                     /locus_tag="ACL8A1_001880"
     CDS             8465..8992
                     /locus_tag="ACL8A1_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HGS1202590.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETISYVFVRVSETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            9069..9425
                     /gene="glnR"
                     /locus_tag="ACL8A1_001881"
     CDS             9069..9425
                     /gene="glnR"
                     /locus_tag="ACL8A1_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HGS1202591.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            9462..10808
                     /gene="glnA"
                     /locus_tag="ACL8A1_001882"
     CDS             9462..10808
                     /gene="glnA"
                     /locus_tag="ACL8A1_001882"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HGS1202592.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDTEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(10904..11023)
                     /locus_tag="ACL8A1_001883"
     CDS             complement(10904..11023)
                     /locus_tag="ACL8A1_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HGS1202593.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            10988..11137
                     /locus_tag="ACL8A1_001884"
     CDS             10988..11137
                     /locus_tag="ACL8A1_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope"
                     /protein_id="HGS1202594.1"
                     /translation="MEKFNFKNNIGQENKLLQIEIYKFTNFCKLQNYTSVNIFSKDIF
                     EAIVN"
     gene            11281..11418
                     /locus_tag="ACL8A1_001885"
     CDS             11281..11418
                     /locus_tag="ACL8A1_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202595.1"
                     /translation="MNPDRAEEYFCRGCQGENPEDIEFYDEQLQAEKVEVLNIRLEVK
                     N"
     gene            11511..11993
                     /locus_tag="ACL8A1_001886"
                     /pseudo
     CDS             11511..11993
                     /locus_tag="ACL8A1_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         3861 a         2179 c         2569 g         3384 t
ORIGIN      
        1 taacaggaca aatcgatcag gacagtcaaa tcgatttcta acaatgtttt agaaatagag
       61 gtgtactatt ctagtttcaa tatactatat atctgcagaa tactctttca gtttcattca
      121 gcctagtctt gcttaggaaa aagtaaaaag ccatccaatt atgatggata gcttcttggt
      181 tcttagattt gttctgcaaa aaccttttca gcaaggttca tagcgtggtc tgatacacga
      241 gtgtagtgtg agatgatgtc gataaagttg accccagctt gtgttgaaca ttcgcctttg
      301 ttgaggcgtt tgatgtgggt ttttctgaga acacgttcta tcttattgat tgcttcatga
      361 cgttctacaa gactgcgtgc tttttcaata tcattgtttt ccacactatc cagagcatct
      421 ttgataaagt cactagtttg gcggtaaact tcctctaatt ctttcaaggc ggcatcagaa
      481 aattcaacat ttttccgttg aagatagtca gtcagattga gtagagcctc cgtgtggtct
      541 ccaatccgtt ccaaatcacg ggaggaatca aggatattgg taagcacttc actttctttt
      601 tggctgagag cttcgcttga aagggcaatg agataacgtg ttaattgctc atcgatggtg
      661 ttaattgctt cttcggtttt atgccctttt tcagcaactt tttcatccaa gtcaatgatg
      721 tacttatagg aaaggtcaaa ggctttagca gcgtagtttc ctaagtgcaa gagctctttc
      781 ttagcatttc ctagagcgat agatggggcc tgtttgatga aatgttcatc aagatataag
      841 ggttcgtatt tgacaacctc gtcctctcca ggaataatct tggttacaaa gtaagccaga
      901 gctccgataa atggaaattg gacaatggtg ttggtaatat taaaggttcc gtgagcaaag
      961 gcgatggtca tttccggtgc tagatttagc gtagcttcaa accaatgaat caggacagta
     1021 aaaggaacta gaaaaataac gcagacgact gttccgataa cgttgaaggc aacatgagct
     1081 cctgctaccc gtttagctgc aatattagcc cctaaagagg caatgatggc tgtaatggtt
     1141 gtcccgatat tgtcaccaaa tagaaccggc aaagctccct gtaggtcaat tagattgccg
     1201 gcgtagaggt tttgtaaaat cccaatggta gccgaagaag cttgaattag caaggtcaag
     1261 ccagtaccga caaagacacc caaaacagga tttttactta gctcaatcat atagtcctta
     1321 aaaacctgta aatccttgag tggagccatt gcgccgctca tgagattgag ggcaaaaaag
     1381 ataccaccga caccaaagag gatgcgtccg atattattga ctgtccgatt ttttgtaaaa
     1441 aagagacaga cggcaccgat aaagagcata ggtagggcat agttacctaa tttaaaaccg
     1501 atgagaaagg atgtgacagt tgtcccaata ttagcaccca tgacaatccc gatagcctga
     1561 cgtaaggtta agagaccggc actgaccagg ccgactgtga taactgttac accagaacta
     1621 gactgaatta gagcagtcat cccaatacca accagaactc caaagaaagg attactagta
     1681 tatttgtcaa tgtaaaaacg aaggcgatct ccagcagctt gttgtaaacc gtctcccatg
     1741 gtcttgatac tatataagaa tagccccaga ccacctaaaa agtgaaataa aatttcctgc
     1801 caattaatgg acatttcttt ttcctccgaa aaataatcgc ggaatatctc ctattctatt
     1861 ttaaaggata aaagtaaatc taacaagtgt taatgtcaag tttgtaaaag aaaattataa
     1921 ttgataagtg tattattttt aagagttttt aaccttattt tagatttgtg aaaacagcta
     1981 ttgttttttg tctaaaaatg ttttaggata aaaatgtgat tatcatgatt ctagagttaa
     2041 aaaactacag aatataaagc gctttcttga aaacaacaaa atcaatcttt taggaggaga
     2101 aaatgaagaa tccatttttt gaaagacgtt gtcgttacag tattcgtaag ttatcagtag
     2161 gagcctgctc gctgatgatt ggtgctgttt tatttgctgg tccagccttg gctgaagaaa
     2221 ctgcagttcc tgaaaatagc ggagctaata cagagcttgt ttcaggagag agtgagcatt
     2281 cgaccaatga agctgataag cagaatgaag gggaacatac tagagaaaac aagctagaaa
     2341 aggcagaagg agtagcgaca gcatctgaaa ctgcagaagc agctagcgca gctaaaccag
     2401 aggaaaaagc aagtgaggtg gttgcagaaa caccatctgc agaagcaaaa cctaagtctg
     2461 acaaggaaac agaagcaaag cccgaagcaa ctaaccaagg ggatgagtct aagccagcag
     2521 ccgaagctaa taagactgaa aaagaagtcc agccagatgt ccctaaaaat acagaaaaaa
     2581 cattaaaacc aaaggaaatc aaatttaatt cttgggaaga attgttaaaa tgggaaccag
     2641 gtgctcgtga agatgatgct attaaccgcg gatctgttgt cctcgcttca cgtcggacag
     2701 gtcatttagt caatgaaaaa gctagcaagg aagcaaaagt tcaagcctta tcaaacacca
     2761 attctaaagc aaaagaccat gcttctgttg gtggagaaga gttcaaggcc tatgcttttg
     2821 actattggca atatctagat tcaatggtct tctgggaagg tctcgtacca actcctgacg
     2881 ttattgatgc aggtcaccgt aacggggttc ctgtatacgg tacactcttc ttcaactggt
     2941 ctaatagtat tgcagatcaa gaaagatttg ctgaagcttt gaagcaagac gcagatggta
     3001 gcttcccaat tgcccgtaaa ttggtagaca tggcaaagta ttatggctat gatggctatt
     3061 tcatcaacca agaaacaact ggagatttgg ttaaacctct tggagaaaag atgcgccagt
     3121 ttatgctcta tagcaaggaa tatgctgcta aggtaaacca tccaatcaag tattcttggt
     3181 acgatgccat gacctataac tatggacgtt atcatcaaga tggtttggga gaatacaact
     3241 accaattcat gcaaccagaa ggagataagg ttccggcaga taacttcttt gctaacttta
     3301 actgggataa ggctaaaaat gattacacta ttgcaactgc caactggatt ggtcgtaatc
     3361 cttatgatgt atttgcaggt ttggaattgc aacagggtgg ttcctacaag acaaaggtta
     3421 agtggaatga cattttagac gaaaatggga aattgcgcct ttctcttggt ttatttgccc
     3481 cagataccat tacaagttta ggaaaaactg gtgaagatta tcataaaaat gaagatatct
     3541 tctttacagg ttatcaagga gaccctactg gccaaaaacc aggtgacaaa gattggtatg
     3601 gtattgctaa cctagttgcg gaccgtacgc cagcggtagg taatactttt actacttctt
     3661 ttaatacagg tcatggtaaa aaatggttcg tagatggtaa ggtttctaag gattctgagt
     3721 ggaattatcg ttcagtatca ggtgttcttc caacatggcg ctggtggcag acttcaacag
     3781 gggaaaaact tcgtgcagaa tatgatttta cagatgccta taatggcgga aattccctta
     3841 aattctctgg tgatgtagcc ggtaagacag atcaggatgt gagactttat tctactaagt
     3901 tagaagtaac tgagaagacc aaacttcgtg ttgcccacaa gggaggaaaa ggttctaaag
     3961 tttatatggc attctctaca actccagact acaaattcga tgatgcagat gcatggaaag
     4021 agctaaccct ttctgacaac tggacaaatg aagaatttga tctcagctca ctagcaggta
     4081 aaaccatcta tgcagtcaaa ctatttttcg agcatgaagg tgctgtaaaa gattatcagt
     4141 tcaacctagg acaattaact atctcggaca atcaccaaga gccacaatcg ccgacaagct
     4201 tttctgtagt gaaacaatct cttaaaaatg cccaagaagc ggaagcagtt gtgcaattta
     4261 aaggcaacaa ggatgcagat ttctatgaag tttatgaaaa agatggagac agctggaaat
     4321 tactaactgg ctcatcttct acaactattt atctaccaaa agttagccgc tcagcaagtg
     4381 ctcagggtac aactcaagaa ctgaaggttg tagcagtcgg taaaaatgga gttcgttcag
     4441 aagctgcaac cacaaccttt gattggggta tgactgtaaa agataccagc ctaccaaaac
     4501 cgctagctga aaatatcgtt ccaggtgcaa cagttattga tagtactttc cctaagactg
     4561 aaggtggaga aggtattgaa ggtatgttga acggtaccat tactagcttg tcagataaat
     4621 ggtcttcagc tcagttgagt ggtagtgtgg atattcgttt gaccaagcca cgtaccgttg
     4681 ttagatgggt catggatcat gcaggggctg gtggtgagtc tgttaacgat ggcttgatga
     4741 acaccaaaga ctttgacctt tattataaag atgcagatgg tgagtggaag ctagctaagg
     4801 aagtccgtgg taacaaagca cacgtgacag atatcactct tgataaacca atcactgctc
     4861 aagactggcg cttgaatgtt gtcacttctg acaatggaac tccatggaag gctattcgta
     4921 tctataactg gaaaatgtat gaaaagcttg atactgagag tgtcaatatt ccgatggcca
     4981 aggctgcagc ccgttctcta ggcaataaca aggtacaagt tggctttgca gatgtaccgg
     5041 ctggagcaac tattaccgtt tatgataatc caaattctca aactccgctc gcaaccttga
     5101 agagcgaagt tggaggagac ctagcaagtg caccattgga tttgacaaat caatctggtc
     5161 ttctttatta tcgtacccag ttgccaggca aggaaattag taatgtccta gcagtttccg
     5221 ttccaaaaga tgacagaaga atcaagtcag tcagcctaga aacaggacct aagaaaacaa
     5281 gctacgccga aggggaggat ttggacctta gaggtggtgt tcttcgagtt cagtatgaag
     5341 gaggaactga ggacgaactc attcgcctaa ctcacgcagg tgtatcagta tcaggttttg
     5401 atacgcatca taagggagaa cagaatctta ctctccaata tttgggacaa ccggtaaatg
     5461 ctaatttgtc agtgactgtc actggccaag acgaagcaag tccgaaaact attttgggaa
     5521 ttgaagtaag tcaggaaccg aaaaaagatt acctagttgg tgatagctta gacttgtctg
     5581 aaggacgctt tgcagtggct tatagcaatg acaccatgga agaacattcc tttactgatg
     5641 agggagttga aatttctggt tacgatgctc aaaagactgg tcgtcaaacc ttgacgcttc
     5701 attaccaagg ccatgaagtt agctttgatg ttttggtatc tccaaaagca gcattgaacg
     5761 atgagtacct caaacaaaaa ttagcagaag ttgaagctgc taagaacaag gtggtctata
     5821 actttgcttc atcagaagta aaagaagcct tcttgaaagc aattgaagcg gccgaacaag
     5881 tgttgaaaga ccatgaaact agcacccaag atcaagtcaa tgaccgactt aataaattga
     5941 cagaagctca taaagctctg aatggtcaag agaaatttac ggaagaaaag acagagcttg
     6001 atcgcttaac aggtgaggtt caagaactct tggctgccaa accaaaccat ccttcaggtt
     6061 ctgccctagc tccgcttctt gagaaaaaca aggccttggt tgaaaaagta gatttgagtc
     6121 cagaagagct tacaacagcg aaacagagtc taaaagatct ggttgcttta ttgaaagaag
     6181 acaagccagc agtcttttct gatagtaaaa caggtgttga agtacacttc tcaaataaag
     6241 agaagactgt catcaagggt ttgaaagtag agcgtgttca agcaagtgct gaagagaaga
     6301 aatactttgc tggagaagat gctcatgtct ttgaaataga aggtttggat gaaaaaggtc
     6361 aagatgttga tctctcttat gcttctattg tgaaaatccc aattgaaaaa gataagaaag
     6421 ttaagaaagt atttttctta cctgaaggca aagaggcagt agaattggct tttgaacaaa
     6481 cggatagtca tgttatcttt acagcacctc actttactca ttatgccttt gtttatgaat
     6541 ctgctgaaaa accacaacct gctaaaccag caccacaaaa cacagtcctt ccaaaaccta
     6601 cttatcaacc gacttctgat caacaaaagg ctcctaaatt ggaagttcaa gaggaaaagg
     6661 ttgcctttca tcgtcaagag catgaaaata ctgagatgct agttggggaa caacgagtca
     6721 tcatacaggg acgagatgga ctgttaagac atgtctttga agttgatgaa aacggtcagc
     6781 gtcgtcttcg ttcaacagaa gtcatccaag aagcgattcc agaaattgtt gaaattggaa
     6841 caaaagtaaa aacagtacca gcagtagtag ctacacagga aaaaccagct caaaatacag
     6901 cagttaaatc agaagaagca agcaaacaat tgccaaatac aggaacagct gatgctaatg
     6961 aagccctaat agcaggctta gccagccttg gtcttgctag tttagccttg accttgagac
     7021 ggaaaagaga agataaagat taaatatcga aaaatcttgt gaaatctttc cttatatttc
     7081 caaagtgtga tataatagtt tcgaataaaa taaataaagg agtttttgta acatggcaaa
     7141 acttactgtt aaagacgttg acttgaaagg taaaaaagtc ctcgttcgtg ttgacttcaa
     7201 cgtaccattg aaagatggcg taatcactaa cgataaccgt atcacagcag ctcttccaac
     7261 tattaagtac atcatcgaac aaggtggacg tgcaattctt ttctctcacc ttggacgtgt
     7321 gaaagaagaa gctgataaag ctggtaaatc acttgctcct gtagcagctg acttggcagc
     7381 aaaacttggt caagatgttg ttttcccagg tgtcactcgt ggtgctgaat tagaagcggc
     7441 aatcaacgct cttgaagatg gacaagttct cttggttgaa aacactcgtt acgaagatgt
     7501 tgacggcaag aaagaatcta aaaacgatcc tgaacttggt aaatactggg catcacttgg
     7561 agatggtatc ttcgtaaacg atgcattcgg tacagctcac cgtgcacacg catctaatgt
     7621 tggtatctca gcaaacgttg aaaaagcagt tgctggtttc cttcttgaaa acgaaattgc
     7681 ctacatccaa gaagcagttg aaactccaga acgtccattc gtggctatcc ttggtggttc
     7741 aaaagtttca gacaagatcg gtgttatcga aaacttgctt gaaaaagctg ataaagtcct
     7801 tatcggtggt gggatgactt acacattcta caaagcacaa ggtatcgaaa tcggtaactc
     7861 acttgtagaa gaagacaaat tggatgttgc gaaagctctt cttgaaaaag caaatggtaa
     7921 attgatcttg ccagttgact caaaagaagc taacgcattt gctggttaca ctgaagtgcg
     7981 tgacactgaa ggtgaagcag tttctgaagg cttccttggt cttgacatcg gtccaaaatc
     8041 tatcgccaaa tttgacgaag ctttgactgg tgccaaaaca gttgtatgga acggacctat
     8101 gggtgtattt gaaaacccag atttccaagc tggtacaatc ggtgtgatgg acgctatcgt
     8161 gaaacaacca ggagttaaat caatcatcgg tggtggtgac tcagctgccg cagcgattaa
     8221 ccttggccgt gcagacaagt tctcatggat tagtacgggt ggtggagcat caatggaact
     8281 tcttgaaggt aaggttcttc caggacttgc agccttgaca gaaaaataag attttataaa
     8341 taaatcaaag aagagaggga tgaaagttcc tcttttcttt tgcttaaaat aaaaacgctt
     8401 cctctcaact attactcata aaaatcaccg atttatgata aaatggaaat agaaagttga
     8461 gattatgagt tattttaaaa aatataaatt cgataaatcc cagttcaaac ttggtatgag
     8521 aacctttaaa acaggtattg ctgtttttct agttctcttg atttttggct tttttggctg
     8581 gaaaggtctt caaattggtg ctttgacagc cgtttttagc ctgagggaga gttttgatga
     8641 gagtgttcat tttgggactt cgcgtattct aggaaatagt atcggtggac tctatgcctt
     8701 ggtcttcttc ttattaaata cctttttcca tgaagccttt tgggtgacct tggtagttgt
     8761 tccaatctgc accatgttaa ccattatgac aaatgtagcc atgaataaca aagcaggggt
     8821 tattggtggt gtagcagcta tgttaatcat taccctatca attccaagtg gtgagacaat
     8881 ttcgtacgtg tttgtgcgtg tatcagaaac gtttatggga gtttttgtcg caattatcgt
     8941 aaattacgat attgatcgta ttcgtctctt tttagagaaa aaagaaaaat aatgttacat
     9001 tttataacat tatcaattga cgtttgtctt tttttagact ataatagaca gaaagaagga
     9061 aattgtaaat gaaggaaaaa gaatttcgcc gaaatatggc tgtttttcct atcggcagtg
     9121 ttatgaagtt gaccgatcta tcggcgcgtc agattcgtta ttatgaagat caagagttga
     9181 tcaagcccga tcgaaacgaa ggaaatcgtc gcatgtattc cttgaatgac atggatcgtc
     9241 tgcttgaaat caaagattat atctctgaag gttataatat cgctgccatt aagaaaaaat
     9301 atgctgaacg tgaagcgaaa tccaagaaag cggttagtca gactgaagta cgtcgtgcac
     9361 ttcacaatga actcctccaa caggggcgtt ttgcttcagt acagtcacct tttggtcgcg
     9421 gttaggcaac cgcaagtagt catagattaa ggagaaaact tatgccaatc acagctgcag
     9481 atattcgtcg tgaagtcaag gaaaaaaatg ttacctttat tcgtcttatg ttctcagata
     9541 ttttgggaac catgaaaaac gtcgaaattc ctgctacaga tgaacagtta gataaggtct
     9601 tgtcgaacaa ggttatgttt gatggatctt ctattgaagg ttttgtacgt atcaatgagt
     9661 cggatatgta cttgtatccg gacttggata catggacagt cttcccttgg ggagatgaaa
     9721 atggaagtgt tgcaggtctg atctgtgatg tctatacaac agaaggtgaa ccatttgcgg
     9781 gtgaccctcg tggtaatttg aaacgagctc ttcgtcacat ggaagaagtt ggattcaaat
     9841 ccttcaacct tggtccagag ccagaattct tcctatttaa gttggatgaa aatggggacc
     9901 caacacttga agtgaatgac aagggtggct actttgattt ggcacctact gaccttgcgg
     9961 acaacacacg tcgtgagatt gtgaatgtct tgaccaaaat gggatttgaa gtagaagcga
    10021 gtcaccacga ggttgcggtt ggacagcatg agattgactt taagtacgat gaagttctcc
    10081 gtgcttgtga taagattcaa atctttaagc ttgttgttaa aaccattgct cgcaaacacg
    10141 gactttacgc aacatttatg gcgaagccaa aatttggtat tgctggatca ggtatgcact
    10201 gtaatatgtc cttgtttgat acagaaggaa ataacgcctt ctttgatcca aatgatccaa
    10261 aaggaatgca gttgtcagaa acagcttacc atttcctagg tggtttgatc aagcatgctt
    10321 acaactatac tgccatcatg aacccaacag ttaactcata caaacgtttg gttccaggtt
    10381 atgaagcgcc tgtttacatt gcttgggctg gtcgtaaccg ttcgccactt gtgcgcgtac
    10441 ctgcttcacg tggtatggga actcgtcttg agttgcgttc agtggatcca atggcgaacc
    10501 cttacgttgc tatggctgtt cttttggaag ttggtttgta tggtattgaa aataaaatcg
    10561 aagcaccagc tcctatcgaa gaaaatatct acatcatgac agcagaagag cgcaaggaag
    10621 ctggtattac agaccttcca tcaactcttc acaacgcttt gaaagctttg acagaagatg
    10681 aagtggttaa agctgctctc ggagatcaca tctatactag cttccttgaa gccaaacgaa
    10741 tcgaatgggc aagttatgca accttcgttt cacaatggga aattgataat tatttagacc
    10801 tttactaata ctaatataga agaaagattg cctgtgggta aacagtcaca cagcagtcta
    10861 agccatcaaa tacctataac ccttaaaata gcgatttcgg tcgctattta tttttcgaaa
    10921 aatcctcttg actcttttgc gaattagatt tatcatcaga cgtgtagaag aaaattaagg
    10981 agtcactatg gaaaaattca atttcaaaaa caatatcggg caagaaaaca agttacttca
    11041 aatcgaaatc tacaagttta ctaacttttg caagcttcaa aattacacat ctgtaaatat
    11101 tttttcgaaa gatatatttg aagccatagt aaattgagac tagaatagta cactacatct
    11161 gctaaaaatt tctagaaact aatttaactt tcctaatcga tttgtttaca tcttatttca
    11221 atctactata ttatctcaat aaatgatgat ggcgactgtt ttgtttgtgc ttgtcgattt
    11281 ttgaatcctg atcgagcgga agaatatttc tgccgaggtt gccaagggga aaatcctgaa
    11341 gatattgaat tttatgatga acaattacag gcagaaaagg tggaggtttt gaatattcga
    11401 ttggaagtaa aaaattgaat tatctacaat ctcatcaaca ataaacctat ttcaacccca
    11461 ttatctatag gcgtattttt acgccttttt atatacttga agcacataag aaacattatg
    11521 ggaagtttgc tgatggaagc actcaagaaa ttgatgttcc ttatgatatt cctgatactt
    11581 gggagtgggt gaggataaaa tcaatttatt ggaattttgg gcaaaataag ccagagaaat
    11641 cctttaggta tatagatacg tctagtattg atagaaaaaa gaatataatc aactacaaaa
    11701 atctacaata tctttcacct gaacaagcgc cttcccgtgc tagaaaatta gtttcgcaga
    11761 atagtgtctt attttcaaca gttagaccat atctaaaaaa tattgctgta gttagagaac
    11821 ttaaagagta tttgatagct agtacagcat ttattgtttt ggatacttta cttaacgaaa
    11881 catatttgaa atattattta ttatcagata attttatcaa tcgtgttaat aataaatcta
    11941 ctggaacaag ttatcctgca atcaatgatt ataattttaa tctattatta att
//
LOCUS       DBERMR010000064        11565 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.064, whole genome shotgun sequence.
ACCESSION   DBERMR010000064 DBERMR010000000
VERSION     DBERMR010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11565
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.064"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            78..1400
                     /locus_tag="ACL8A1_001887"
     CDS             78..1400
                     /locus_tag="ACL8A1_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGS1202596.1"
                     /translation="MNNILAFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEAFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRMFIQRGITIKMPSGVPDVVSKSFSALLPGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTTENQEA
                     VAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPFIATPTINALITYVSMAVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
     gene            1402..3288
                     /locus_tag="ACL8A1_001888"
     CDS             1402..3288
                     /locus_tag="ACL8A1_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HGS1202597.1"
                     /translation="MVIRVFDQQKNTYSSFALEELSYYMNRVFKTNIELVEEKEADIF
                     VGLVNKEDRKDHVLISLDKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRRHDFVP
                     ELRFEDFLDKQLSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEKFSNELVQELSDRLDKELQKRGLIHHRVGHGWTAEVLGY
                     SSKFGWESGLSISEEKKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRILNQLDRALTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYLRNKIILP
                     NSLEENLSYLFEWQKTFKGDSFVYDYPLGRAHYGDLGYMKISQTIYRDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGEMLWKKTRSYEELIEEYFSALYGSNWQSVVEYLEKL
                     SSYSSCDYFNAIGSRQNDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            3456..3800
                     /locus_tag="ACL8A1_001889"
     CDS             3456..3800
                     /locus_tag="ACL8A1_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGS1202598.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEASGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            3810..5222
                     /gene="lacG"
                     /locus_tag="ACL8A1_001890"
     CDS             3810..5222
                     /gene="lacG"
                     /locus_tag="ACL8A1_001890"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HGS1202599.1"
                     /translation="MENLQVKALPKEFLLGAATAAYQVEGATRVDGKGTNMWDVYLQE
                     NSPFLPDPASDFYYRYEEDIALAAEHGLQALRLSISWVRIFPDIDGEPNILAVRYYHR
                     VFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGKIGC
                     IHALKPGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSDDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAPLNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSALWVKGLKRN"
     gene            5292..6971
                     /locus_tag="ACL8A1_001891"
     CDS             5292..6971
                     /locus_tag="ACL8A1_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGS1202600.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIAWMRKVYMLTMGVLGVIVSGTVAKSLAGNVNRKMPPGKV
                     INDTSAMLAAICGYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSAFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDTFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGAESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWTFPGPLGLLIGTNF
                     QLISFVFLSLILVVDILIYLPFCRAYDRQLLVKEDIASSNDIILEEDTSEIMPGEIDE
                     IKSKELKVLVLCAGSGTSAQLANAINEGANLTEVRVIANSGAYGAHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKSPSKALQFVLEHYQAV"
     gene            complement(7097..8536)
                     /locus_tag="ACL8A1_001892"
     CDS             complement(7097..8536)
                     /locus_tag="ACL8A1_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HGS1202601.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(8540..9889)
                     /gene="trkA"
                     /locus_tag="ACL8A1_001893"
     CDS             complement(8540..9889)
                     /gene="trkA"
                     /locus_tag="ACL8A1_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HGS1202602.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            10078..10926
                     /locus_tag="ACL8A1_001894"
     CDS             10078..10926
                     /locus_tag="ACL8A1_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HGS1202603.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     DQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            10941..11489
                     /locus_tag="ACL8A1_001895"
     CDS             10941..11489
                     /locus_tag="ACL8A1_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HGS1202604.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLVGYGLWWTWIIASGLFGLVVGLFRKYVRVINS
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
BASE COUNT         3507 a         1899 c         2515 g         3644 t
ORIGIN      
        1 caacaaaaaa tatattgaca acgctttctg ttagtggtat atttaattta taaaaaataa
       61 aaaaaggagt tccagtgatg aacaatattt tagcgttttt agaaacaaaa gtcgctccgt
      121 ttggtgaaaa agttggcaac caacggcatt tgaaagctat tcgtgaagga tttatgatgg
      181 caatgccttt gattttagtc ggctctttat ttcttattct aatcagttgg cctcaagagg
      241 cttttacaaa ttggctgaat agtgttggat tgctaagtat cttgacaact atgaatcagt
      301 caacagtagc gattatctcc ttggtcgctt gtttcggtat tgcctacagg ttgtcggaag
      361 gatatggtac agatggtccg tcggcaggga tcatagcctt atccagtttt gtattgatgg
      421 cacctcgttt ttcgagtatg gtttatgata aaaatgggga gcaggtcaag cagttatttg
      481 gcggcgcaat accattttct agcctgaatg catcttcttt gtttatggcg attactattg
      541 gattggttac agcagagatt tatcgtatgt ttatccagcg cggaattacg ataaaaatgc
      601 caagtggtgt cccagatgtg gtaagtaaat cattttcagc tcttttacct ggctttacta
      661 cttttgtttt gtgggctctg gtcttaaaag gtcttgaagc ggcaggagtt gcaggaggtc
      721 tcaacggact cctaggtgca attgttggaa caccgcttaa gttaattgca ggaacgcttc
      781 caggtatgat tctatgtgtt attgtaaact cattcttttg gttctgtgga gttaatgggg
      841 gacaagtttt aaatgctttt gtagacccag tttggttaca atttactaca gaaaaccaag
      901 aagctgtggc tgcaggacaa acactccaac acattattac attaccgttt aaagatttat
      961 ttgtatttat tggtggcggt ggagcgacta ttggtcttgc gatttgtctc ttcctattta
     1021 gtaagagtcg tgcgaataaa acattaggta agctagctat tataccgtct atttttaata
     1081 tcaatacagc tattctattt acgtttccaa cagttttaaa tccgattatg ctgattccgt
     1141 ttattgctac tcctacaatc aatgccttga ttacctatgt atcaatggct gtaggattag
     1201 taccctatac aacaggtgta atccttccgt ggacaatgcc accgatcata ggaggcttcc
     1261 ttgcgacagg agctagttgg cgaggagctc tattacaagt tgttttgatt ttggtttctg
     1321 tagcaattta ttatccattt ttcaaaattg cagataaacg caatcttgaa aaagaaaaag
     1381 ctactgttgg agggaaataa gatggttatc agagtatttg atcaacagaa aaatacttat
     1441 tctagctttg ccttagagga attaagttac tatatgaatc gggtctttaa gactaacata
     1501 gagcttgtcg aggagaagga agcggatatt tttgtaggat tagtcaataa agaggacaga
     1561 aaagaccatg ttcttatctc attagacaag ggtaagggga gaattgagtc taatacaatt
     1621 gtaggtttac ttattggaat ttaccgaatg tttcatgaat ttggggttgt gtatactaga
     1681 ccagggcgca gacatgactt tgttccagag ttacgatttg aagatttttt agataaacag
     1741 ctatctatag atgaaacagc cagttactat cataggggag tatgtataga gggagcggat
     1801 tcatttgaaa atatactaga tttcattgat tggctaccta agattgggat gaacagtttt
     1861 ttcatccagt ttgaaaatcc ttactctttt ttgaaacgtt ggtatgaaca tgagtttaat
     1921 ccatacctaa ataaagaaaa attctcaaat gaattagtac aagaattgag tgataggttg
     1981 gataaagaat tgcaaaaaag aggtcttatt catcatcgtg ttggtcatgg atggacagct
     2041 gaggttttag gttactcttc aaaatttggc tgggaatcag gtcttagtat ttcagaggag
     2101 aagaaaccgt atgtcgctga aataaacggg aaacgagaat tatttaatac ggctccgatt
     2161 ttaaccagtc tggatttttc aaatccagat gtggcggata agatggtaga aattatcaag
     2221 gattatgcca agaaaagacc tgatgttaac tacttacatg tatggttgtc ggatgctcgt
     2281 aataatattt gtgaatgcga aaactgtaga caagaattgg tttcggatca gtatattcgt
     2341 attctcaatc aattggatag ggctttaacg agtgagggat tagatacaaa gatttgtttt
     2401 ctgctttacc atgagttgct atgggcgcct cagaaagaaa aattagataa tcctgaacgc
     2461 tttaccatga tgtttgcacc gattacaaga acatttgaaa tgagttatgc agatgtagat
     2521 tttgacaatt ccatacctac gcctaaacct tatctgcgta ataaaattat acttccgaat
     2581 tctcttgagg aaaatttatc ttatcttttt gagtggcaaa aaacatttaa aggagatagt
     2641 ttcgtatatg actatccttt agggcgtgct cattatggcg atttaggcta tatgaaaatt
     2701 agtcaaacca tttacagaga tgtatcttat ctttctaacc tacatttgaa cggttacatt
     2761 tcgtgtcaag aattacgtgc cggattccct cataattttc ctaattatgt tatgggggaa
     2821 atgctctgga agaagacaag aagttatgaa gaattgattg aagaatactt ttctgctttg
     2881 tatgggtcaa attggcagtc tgttgttgaa tatttagaaa aattatccag ttattcctca
     2941 tgtgattatt ttaatgcaat tggcagtcgt caaaatgatg ttttagcgaa tcattattat
     3001 atagcttaca atctagctga taattttttg ccaattattg aggaaaatat ttctaagtta
     3061 ttaaatagtc aaaaggatga atggaaacag ctcagttatc atcgtgaata tgttgttaag
     3121 atggcgaagg ctttatatct tcaagcaacc ggaaaaacaa ggcaagctca agatgaatgg
     3181 agaaatgtgt tgaattatat ccgtgggcac gaactgctat tccaatctaa tttggatgtt
     3241 tatcgtgtaa ttgaagtagc aaaaaattac gctggtttcc acttataaat cataagtata
     3301 gaaaatgaac caaggtattc agagaagatt gatcctaaat attatgaaat ttaaggattt
     3361 ttaagatatt tagggtcgac tttctattta tatcgtagcg aagtcatttt aataatgatg
     3421 tgtaaaagat ggatcaagat tgaggaggaa gaaagatgaa atcaaaagaa gaaataaata
     3481 tgcttggttt tacaattgtc gcttacgcag gagatgcaag gtcagatttg atggatgctt
     3541 tggcgtttgc gagagatgga tattttgaac aggcaagaga attggttgag tctgcaaacg
     3601 actcaatagt gtctgcccat cgagaacaga ctaatttatt agcggaggag gcatctggag
     3661 ataattttga agtgagtttt atcatgattc atggtcaaga tactttgatg acaacgatgc
     3721 tattgtatga tcaggtcaag ttttttattg atgaatatga acgaattcga aagattgaag
     3781 aacatattgg tttgcaatga ggattagtca tggaaaattt acaggttaaa gccttaccga
     3841 aggagttttt attaggagct gctaccgctg cttatcaagt agagggtgca actagggtag
     3901 atggcaaagg aacaaatatg tgggatgttt atttgcaaga aaacagtccg ttcttaccag
     3961 atccagctag tgatttttat tatcgttacg aagaggatat agctttggcg gcagaacatg
     4021 gtttgcaggc tttgcgttta tctatttctt gggttcgtat atttcctgat atagatgggg
     4081 agcctaatat attagctgtt cgttattacc atagagtctt tcagtcttgc ttaaaacata
     4141 atgtgattcc gtttgtttct ttacatcatt ttgattcgcc tcagaaaatg ttagaaacag
     4201 gggattggtt gaatagagag aatattgatc gtttcatacg atatgctcgc ttttgtttcc
     4261 aagaatttac agaagtcaag cattggttta caatcaatga actgatgtct cttgctgcag
     4321 gtcaatatat aggaggtcag tttcctccaa atcatcattt tcaattatct gaagcaattc
     4381 aagcgaatca taatatgttg ttggcgcatg ctcttgcagt cctcgaattt catcaattag
     4441 ggattgaggg aaagataggt tgtattcatg ctttaaagcc aggctatcct attgatgggc
     4501 aaaaagaaaa tattttagca gctaaacggt atgatgttta taataataaa tttctattag
     4561 atggaacttt tttgggctac tacagtgatg acacgctttt tcacttgaat caaatattgg
     4621 aagctaataa ttctagcttt attattgaag atggtgattt agaaattatg aaaagagctg
     4681 cacctcttaa tacgatgttt gggatgaatt attatcgttc agaatttatt cgtgaataca
     4741 aaggtgaaaa tagacaagaa tttaattcaa caggaataaa aggacagtct tcttttaaat
     4801 taaatgctct aggtgaattt gtaaaaaagc ctggtattcc gacaacagat tgggattgga
     4861 atatttatcc tcaagggtta tttgatatgt tgcttcgtat caaagaagaa tatcctcaac
     4921 atccggtcat ttatttaact gaaaatggta cagcccttaa agaggttaag ccagagggcg
     4981 agaatgatat tattgatgac agtaaaagaa tccgttatat tgagcaacat ttacacaaag
     5041 ttttagaggc tcgagatagg ggagtcaata ttcaaggcta ttttatatgg tctttgcaag
     5101 atcaattttc ttgggcgaat ggctacaata agcgatatgg tcttttcttt gttgattatg
     5161 aaacacagaa gagatatatt aagaaaagtg ctctttgggt aaaaggatta aaacggaatt
     5221 aaggttagcg atttgactga tgtttaatat attttaacta tgagcttaaa ttttttacag
     5281 gaggagtttt tatggataag ttagtcgctg ccattgaaaa gcaacaaggg aaatttgaaa
     5341 aaatttctac taataattat atgatggcta ttaaagatgg atttattgct actatgcctt
     5401 taattatgtt ttcaagcttt ttgatgatta ttattatgat tcctaaaaat ttcggagtag
     5461 agttaccgag tccagctatt gcctggatga ggaaagtgta tatgctaacc atgggagttt
     5521 tgggtgttat tgtttcaggg acagttgcaa agtcattggc tggaaatgtt aatagaaaaa
     5581 tgcctcctgg aaaggtaata aatgatactt ctgcaatgtt ggcggccata tgtggctatc
     5641 ttgtattaac tgtaacgctt gtagttgatg agaaaacggg atctacaagt ttgtcgacaa
     5701 actatttagg atctcaagga ttgataactt cgtttgtcag tgcctttatt actgtaaatg
     5761 tttaccgatt ctgtattaag cgagacatta ctattcattt acctaaggaa gttcctgggg
     5821 ctatatcaca agcttttaga gatactttcc ctttttcttt tgttttactt attagtggtt
     5881 tgttagatat tgtatctcgg tttagtttag atgttccttt tgcccaagta tttcaacaac
     5941 tattgactcc tatttttaag ggggcagaat catatcctgc tatgatgttg atttggttta
     6001 tgtgtgcttt gctttggttt gttggaattc atggaccatc tattgtctta cctgctgtta
     6061 cagctttgca actgagcaat atggaagaga atgctcaact tcttgcaaat gggcagttcc
     6121 cttatcattc tttaacacct aatttcggga attatatcgc tgctattgga ggaacggggg
     6181 ctacctttgt tgtaccattt attttgattt tctttatgcg gtctaaacaa ttaaaatcgg
     6241 taggtaaagc tacaattact cctgttttat ttgcggtaaa tgaacctctt ctatttggta
     6301 tgcctgttat tttgaatccc tatctttttg tccctttttt gatgactcca ccagtgaatg
     6361 tatttctagg aaaggtattt attgatttct ttggaatgaa tggattttat atccagttac
     6421 cttggacctt tcctggtccc ttgggattgt taattggaac gaattttcaa cttatctcct
     6481 ttgtattttt atctttgatt ttagttgtcg acatattgat ttatttgcca ttctgtagag
     6541 cgtatgatag acagttactg gtgaaagaag atattgcaag ctcaaatgat attattttag
     6601 aggaggatac aagtgaaata atgcctggtg agatagatga aataaaaagt aaggagttga
     6661 aagtactggt tctttgtgca gggtctggaa caagtgcgca attagccaat gcaattaacg
     6721 agggggctaa cttaacagag gttagagtga ttgcgaattc aggagcgtac ggagctcatt
     6781 atgatattat gggtgtttat gatttaatta ttctggcccc acaagttcgg agttattata
     6841 gagagatgaa ggtggatgca gaaagattag gtattcagat agttgctacc agaggaatgg
     6901 aatatattca tttaacaaag agtccaagta aagccttaca atttgtattg gagcattacc
     6961 aagctgtgta gtaagttttt ccatctttta tttgagtaaa gattttgttt acagataggc
     7021 ttggatttaa aaacgttccc ccttttttaa gataagaatc cctctttcac aattgtaaaa
     7081 agagggattt tgtattttat ctcttagacc aagttctctt cataaagaga aggaggattg
     7141 ggtaaatctc caagcgccct gcaatcattg caaaggatag gagaattttt gagatgggac
     7201 taaagattga gaaactagaa gtggttccta gaataggccc gatattattg aaacagctaa
     7261 agacagcgct ggtcacgacc agaaaatcat tgctatctag gctgacaata aagataagcg
     7321 ctagcaaaat catagcatag atgacaaagt acttgagaat cttatgctgg gtatctttgt
     7381 caatcaccgt tttattaaca tggagggtca aaacacggtg gggcgatagg attgacaaaa
     7441 tttggttttt ggcaattttt gaaaggatga ggcctcgaat aatcttgagt ccacctgcag
     7501 ttgatccagc agagccaccg attgccatga ggaaaaggag gataaactgg gagaagaggg
     7561 gccagttggt aatatctcca tatccaaaac cagttgttgt aatgatgttg gaaacctgga
     7621 agaaggtcat ttcaaagctc tttgaaaacc ctgggtagag gtagagggtg ttgaggctaa
     7681 tcaagcctgt agaaaccagt acaatgacca agtaagccct aagctcttca tctccaaaga
     7741 aggccttgat gcgacggagc atgaggtagt agtagaggtt gaaatttact ccaaaaacca
     7801 gaactccgat actgaccaga taggtaatca gtgagctgcc atagtgggca attccgtcgt
     7861 tatagacggt aaagcctcca gttcccgctg tccccatagc aataacaaaa ctatcgtaga
     7921 gaggcatacc ggctagataa tagatgatga caaagaggga gaagagagct agataaagga
     7981 gatagagaat ctgggcagtg ttttttagtt tggatacaac cttgccaaaa acaggacctg
     8041 gaacctcagc cttcatcacc tctaggtggc tatttttggc attgtccata atagcaagtg
     8101 caaaaacaag cactcccatc cctccaatca agtgggtaaa acttcgccag aagaggaggg
     8161 aacggctgag aaccgaaacg tcgttcaaaa tacttgctcc agtagttgta aatccagaac
     8221 taatttcaaa aaaggcatca ataaggctgg ggatttgccc agaaaagaca aaggggagac
     8281 caccaaagaa agaccaaagg atccaacaga gggcaacgat caagactccc tccttggcat
     8341 aaatccgttg attttttggc ttctgtaaac tccctgaacc gcctaacaat acgagaatcc
     8401 ctatggtcga aaagagggct gtaaagactt ggctcgattc acggtaatag acagcaatcg
     8461 caacaggaac caaaagaaga acagcttcaa tcaaaagtaa ttttgaaagg aggtaacgaa
     8521 tcatactttt attcatttct tacctcgcga tcaagtcata aatcttggtg atgtttggca
     8581 acaaggttgt tactaggagc ttgtctccaa cttccaacat atcctcccca gttgggaaaa
     8641 tagtcttgcc ctttcgaata atggctgcaa taagaacccc ttttttcaat ttcagttgag
     8701 aaagaggttt ggcagtcatt ttattggctt ccttgatatg gaattgcagg gtttcgattt
     8761 ggccattggc tagatggtgc atagcttgaa ggtctgaata ctgggcatta actcgaccac
     8821 gaataaagtg cataatcgta tctacagcga tgcttttagg tgtgatgata cttgaaaaat
     8881 caggcgcatt gataatctcg aggagactgg tacgattgac cttagtaata tttttctgta
     8941 cacctaccct gtcaaggaac atagatgtaa tcagattttc ctcatcgact cctgttagag
     9001 tcgcaacggc atcatagtgt tgagcacttt cttccagcag gatatctttt gcggttccat
     9061 ctccttgaac gatgtagaga tttgggaatt tctcgctaaa gaagctggcg atttcaggat
     9121 tgatttcaat gacttttgta tcgatacgac tatctttgag aataccaagt agataatagg
     9181 caattctacc tgccccaacg atgagaaggc tcttcacggc gcgtgattta aaataattat
     9241 ggaagagtat catatcgaca cggttaccag tgacaaagat tctatcttta tcctgtacag
     9301 tcatgtcacc gcttggaatg ataatttgat gatccctctc tatcgcacag acaatgacat
     9361 taccaaattt tttacgaaaa tcagaaatgg gcatttggca aagaccgctg gtggacttga
     9421 cgacaaattc catgaggcta acgcgtccac cagcaaagcg ttcgacagac agggcgttgg
     9481 ggaagtcaat gatattcgcg atagcgcggg cagccaagag ctcaggatta acgataagag
     9541 aaaaaccgag aatattcttt tccttgaaat aagagttaga atattcaggg ttccgcaccc
     9601 gaacgatagt ttctttagct cccattttct tggctagaac tgctgcaatc atgttgactt
     9661 catcgtgctc agtcagggcg ataaagatat cacaatcttg gacgctggct tgctcaagaa
     9721 tggcaaaatc ggccccatta ccaaggatac caatgatatc aaagcgactg acaatatgat
     9781 tgagaacagc ttcgtcttgc tcaatcagca aaacatcatg cttttctgca accaaggagc
     9841 gacagagggc aaaaccaact tttccccctc cgacaaggat aattttcata ataaaaccta
     9901 ctttttcatg atgtaactat catacccttt ttcaagaaaa aatgcaccta ctagctaata
     9961 acaagaactt ttagtaggaa atttgctata aggtaaaact ataccctaac caattgaaat
    10021 agctattagc gactttctct gaaatatggt atgataaagg atatacaagg agataaaatg
    10081 aataataatt tactggtatt acaatcagac tttggtctgg ttgatggtgc ggtatcggct
    10141 atgattggag tggctttaga agagtctcca accttaaaaa tccatcactt gacgcacgat
    10201 atcacgcctt ataatatttt tgaggggagc tatcgtctct ttcagacggt ggattactgg
    10261 cctgagggaa cgacgtttgt atcggttgtc gatccaggtg tcggttcgaa acgtaagagt
    10321 gtagttgcca agactgcaaa aaatcaatac attgtcacgc cagataatgg gacgctttcc
    10381 tttatcaaga aacacgttgg cattgtagcc attcgtgaga tttctgaggt ggccaatagg
    10441 cgtcaaaaca cagagcattc ttataccttc cacggtcgtg atgtctatgc ctatactggt
    10501 gctaaactgg ccagtggtca cattactttt gaggaagtag ggccagagct cagtgtggac
    10561 cagattgtag agcttccagt cgtagcgacc atcatagaag atcatctggt gaagggagcc
    10621 attgatattc tggatgtgcg tttcggttcg ctttggacct ctatcacacg ggaagaattt
    10681 tacaagctgg aaccagaatt tggtgatcgt tttgaagtga ccatctatca tgctgatatg
    10741 ctggtctatc aaaatcaggt tgtctatggc aaatcatttg cagatgtgag aattgggcaa
    10801 cccatccttt acatcaactc tctctatcgt ttaggtttgg ctatcaacca aggttccttt
    10861 gccaaggcct ataatgtagg tgtcggttca tcttggacca ttgaaataaa gaaaatagaa
    10921 ggataaaata ggagaatagt atggaaatca aatttacaat taaacaagtt gttgctgtcg
    10981 gaattggcgc tgccctcttt gtcgtcatcg ggatgatcaa cattccaacc cctgttccaa
    11041 atacaagcat ccagcttcag tatgcggtac aagcgctact ttctattatt tttggaccga
    11101 ttatcggttt gcttgtcggg ttgattggtc atgcaatcaa ggactctctt gttggttatg
    11161 gtctatggtg gacttggatt atcgctagcg gactctttgg tttagttgtg ggacttttta
    11221 gaaagtacgt tcgcgtgatc aatagtgttt ttgactggaa agatattctt atttttaatc
    11281 tcattcaact acttgcaaat gcccttgttt ggggtgtctt ggcaccactt ggagatgttg
    11341 tgatttatca agaagcggca gaaaaagtat ttgcacaagg gattgttgcg ggaattgcca
    11401 atggtgtatc tgtagctatt gcaggaactc ttctcttact tgcctatgca ggaacccaaa
    11461 ctcgtgcagg aagtttgaaa aaggactaat actcaatgaa aatcaaagag caaactagga
    11521 agttagccgc aggttgctca aaacagtgtt ttgagattgt ggata
//
LOCUS       DBERMR010000065        11387 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.065, whole genome shotgun sequence.
ACCESSION   DBERMR010000065 DBERMR010000000
VERSION     DBERMR010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11387
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.065"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            106..351
                     /locus_tag="ACL8A1_001896"
     CDS             106..351
                     /locus_tag="ACL8A1_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202605.1"
                     /translation="MKAFYITIEKFIRDDESLKCYFIKVISSRSKVTSLDQIEADKTI
                     QRKYSSELKKFIGFYNEIICEENSFLHVRKRWSSWFR"
     gene            752..1744
                     /locus_tag="ACL8A1_001897"
     CDS             752..1744
                     /locus_tag="ACL8A1_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS1202606.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPNSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1746..2564
                     /locus_tag="ACL8A1_001898"
     CDS             1746..2564
                     /locus_tag="ACL8A1_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1202607.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVIYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2566..3351
                     /locus_tag="ACL8A1_001899"
     CDS             2566..3351
                     /locus_tag="ACL8A1_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1202608.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWALPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3390..3668
                     /locus_tag="ACL8A1_001900"
     CDS             3390..3668
                     /locus_tag="ACL8A1_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HGS1202609.1"
                     /translation="MFVIEEVKDENQKKAVVAEVLKDLPEWFGIPESTQAYIEGTTTL
                     QVWTAYQESDLTRFVSLSYSSEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            3653..3850
                     /locus_tag="ACL8A1_001901"
     CDS             3653..3850
                     /locus_tag="ACL8A1_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1202610.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFPQLWNPQNPCQILIKKLE"
     gene            complement(4168..4305)
                     /locus_tag="ACL8A1_001902"
     CDS             complement(4168..4305)
                     /locus_tag="ACL8A1_001902"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202611.1"
                     /translation="MKIKEQTRKLVAGCSKHGFEVVDRTDVVSNHTHGKATLTWFEEI
                     F"
     gene            4538..10957
                     /locus_tag="ACL8A1_001903"
     CDS             4538..10957
                     /locus_tag="ACL8A1_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HGS1202612.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSNSEDALNISDKEKV
                     AENKEKHEDIHSAMETSQDTEEKKTTIIEEKKVVSKNPVIDTKTSNEEAKIKEENSNK
                     SQGDHTDSFVNKNTENPKKEDKVVYIAEFKDKESGAKAIKELSSLKNTKVLYTYDRIF
                     NGSAIETTPDNLDKIKQIEGITSVERAQKVQPMMNHARKEIGVEEAIDYLKSINAPFG
                     KNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKIPHAF
                     NYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQIFSY
                     KMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRALRKAG
                     IPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQTVEFD
                     KVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDLRGKI
                     AVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGTKSQV
                     FSISGDDGVKLWNMINPNKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVGDEKE
                     IDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVINGKST
                     YGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQNTAR
                     PMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGSISLK
                     EIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGKQIVP
                     EIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESVEEME
                     ALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGKPKIP
                     GTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSSGSKIA
                     NIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVISREHF
                     IRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATKIRGQ
                     FEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLITKDTY
                     HKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYAGEGQ
                     GRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANQADEKGMISY
                     YLVDPDQDSSKYQKLGEIAESKFKNLGNGKEGSLKKDTTGVEHHHQENEESIKEKSSF
                     TIDRNISTIRDFENKDLKKLIKKKYKEEDDFVTGGKRTVELDYKYDDKGNITAYEDES
                     ALEYETEKFDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNNYKFIEIKATK
                     YDSHSKTMIFDLYANINDIVDGLAFAGDMRLFVKDDNQIKAETKIRMPEKNKETKAEY
                     PYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKGYALKV
                     TTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRSTQAVLM
                     SALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKSNVEA
                     IKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNWELRVN
                     ESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMDGNVYL
                     QTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKRNNGFD
                     GEIQEQHIYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDTGEVSE
                     LKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKKDAGYV
                     INLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDLQREDH
                     SQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIVGIFLG
                     LKKKNQD"
BASE COUNT         3987 a         1712 c         2278 g         3410 t
ORIGIN      
        1 ccaatcgatt tgttctcatc ttatttcaat ccgctatata ttatggtatc gaatcttcat
       61 cagaatgata aaattaatca attgatatct gattacaaac agaatatgaa agctttttat
      121 atcactattg aaaaatttat acgagatgat gaaagcctta agtgttattt tataaaggtt
      181 atttcaagtc gttccaaggt aacaagtcta gatcagattg aagctgataa aacgatacaa
      241 agaaaatatt caagtgagct aaaaaaattt attggatttt ataatgagat tatttgtgag
      301 gaaaatagtt tcctacatgt acgaaagagg tggtcgagtt ggtttaggta gtcgatgcgt
      361 gagttgataa ttctcagggt atggacttct ttttcatgaa tgaggtaaaa gagcaggtat
      421 tgtttagaga caatcattct gagcatattt tctggataga gggagtatcc gattttatga
      481 tcaaagttaa taccgccctc tggtgagaag atgagtaggt tggtaattta aactattaaa
      541 cagaattttt gattaaaagt attattttat gagagaaatc ctaatttcac aatccatagg
      601 caaacgcttg catttcgttt tttattggac tataataggt tggtataaag ccttttgtag
      661 taataaaatg tagaaggtgt agaaagcaag gatttagaat atttgtagtt aaaaacacaa
      721 tgttgctatt ccttacgata gggagataga tatggcaatg atagaagtgg aacatcttca
      781 gaaaaatttt gtgaagactg ttaaggaacc gggcttgaag ggggctttgc gctcctttat
      841 tcatcctgaa aagcagacct ttgaagcggt caaggatttg acctttgagg ttccaaaagg
      901 gcagatttta ggatttatcg gggcaaatgg tgctgggaag tcgacaacca ttaaaatgct
      961 gacaggaatt ttgaaaccaa catctggttt ttgtcggatt aacggcaaga ttccccagga
     1021 caatcggcaa gattatgtca aagatattgg cgtagtcttt ggacaacgca cccagctatg
     1081 gtgggatttg gctctgcaag agacctacac tgtcttaaaa gagatttatg atgtgccaaa
     1141 ctcgctcttt cataagcgca tggacttttt gaatgaagtc ttggatttga aggactttat
     1201 caaggatccc gtgcggactc tttcactggg acaacggatg cgggcggata ttgcggcctc
     1261 cttgctccac aatcccaagg ttcttttttt agatgagccg accattggtt tggacgtttc
     1321 ggttaaggat aatattcgtc gggcaattac tcagatcaat caagaggaag aaactaccat
     1381 tcttttgacc actcacgatt tgagtgatat tgagcaactt tgtgatcgga ttttcatgat
     1441 tgacaagggg caagagattt ttgatggaac ggtgagccaa ctcaaggaga cctttggtaa
     1501 gatgaagact ctctcttttg aactgctacc aggtcaaagt catctcgtct ctcactatga
     1561 aggtctgtct gatatgacca ttgatagaca aggaaacagc ctcaacattg aatttgatag
     1621 ttctcgctac cagtcagctg acattatcaa gcaaaccctg tctgattttg aaatccgcga
     1681 tttgaagatg gtggatacgg atattgagga tattatccgt cgcttctacc gaaaggagct
     1741 ctaggatgat caaattgtgg agacgttata aaccctttat caatgcaggg gttcaggagt
     1801 tgattactta ccgagtcaac tttattctct atcggattgg cgatgtcatg ggggcttttg
     1861 tggcctttta tctctggaag gctgtctttg attcttcgca agagtctttg attcagggct
     1921 tcagtatggc ggatatcacc ctctacatca tcatgagttt tgtgaccaat cttctgacta
     1981 gatccgattc gtcctttatg attggggagg aggtcaagga tggctccatt atcatgcgtt
     2041 tgttgcgacc agtgcatttt gcggcctcct atcttttcac cgagcttggt tccaagtggt
     2101 tgatttttat cagcgttggc cttccatttt taagtgtcat tgtcttgatg aaaatcatat
     2161 cgggtcaagg tattgtagag gtgctaggat taactgtcat ttatcttttt agcttaacgc
     2221 tcgcctatct gattaacttt ttctttaata tttgctttgg attttcagcc tttgtgttta
     2281 aaaatctttg gggttccaac ctacttaaga cttccatagt ggcttttatg tcggggagtt
     2341 tgattccctt ggcattcttt ccaaaggttg tttcagatat tctctccttt ttgccttttt
     2401 catccttgat ttatactcca gttatgatca ttgttggaaa atacgatgcc agtcagattc
     2461 ttcaggcact ccttttgcag ttcttctggc tcttagtgat ggtgggattg tctcagttga
     2521 tttggaaacg ggtccagtcc tttatcacca ttcaaggagg ttagtatgaa aaaatatcaa
     2581 cgaatgcatc tgatttttat cagacaatac atcaaacaaa tcatggaata taaggtggat
     2641 tttgtggttg gtgtcttggg agtctttctg gctcaaggct tgaatctctt gtttctcaat
     2701 gtcatctttc aacatattcc attcctagaa ggctggacct ttcaagagat agctttcatt
     2761 tatggatttt ccttgattcc caagggaatg gaccatctct tttttgacaa tctctgggca
     2821 ctagggcaac gcctagtccg aaaaggggag tttgacaagt atctgactcg tcccatcaat
     2881 cctctctttc atatcctagt tgaaaccttt cagattgatg ccttgggtga actcttagtc
     2941 ggtggtattt tattgggaac aacagtgacc agcattgttt gggctcttcc aaaattcctg
     3001 cttttcctag tttgtattcc ttttgcgacc ttgatttata cttctcttaa aatcgcaaca
     3061 gccagtatcg ccttttggac taagcagtca ggcgccatga tttacatctt ctatatgttc
     3121 aatgactttg ctaagtatcc gatttctatt tacaattctc ttcttcgttg gttgattagc
     3181 tttatcgtgc ctttcgcctt tacagcctac tatccagcta gctatttctt acaggaaaag
     3241 gatgtgttct ttaacgtagg aggtttgatg ttgatttctc tggttttctt tgttatttcc
     3301 cttaaacttt gggataaggg cttagattcc tacgaaagtg cgggttcgta aaagctaaag
     3361 taagactaaa atcaagaaag gaacttatga tgtttgtaat tgaagaagtc aaggatgaaa
     3421 atcaaaaaaa ggcagttgta gctgaggttt tgaaggattt gccagaatgg tttggaatcc
     3481 cagaaagcac acaagcctat atagaaggaa ccacgacact gcaagtttgg accgcctatc
     3541 aggagagtga tttgactaga tttgtaagct tatcctattc gagtgaagat tgtgcagaga
     3601 ttgattgtct cggcgtaaaa aagcttatca aggtagaaaa attgggagcc aattgcttgc
     3661 tactttagag agtgaagctc gtaaaaaagt tggttatctg caggtcaaaa cagtggcaga
     3721 aggttctaat aaagattatg atcgaacaaa tgacttttat cgaggtcttg gctttaaaaa
     3781 gttagagatt tttcctcaac tatggaatcc gcaaaatcct tgtcagattt tgattaaaaa
     3841 gcttgaataa tattacttga catctattct cagagtgcta tactgtaagt gtaatcgccg
     3901 atttagctca gttggtagag caaggcactc gtaaagccta ggttataggt agataaacga
     3961 ctgaggattt gaaaaaatag ataggtagaa gataaccgtt aagccttatt cttagcggtt
     4021 atttatattg tttaatagcg ctgatatttc atcaattatg cctgttttcg tgtttctggt
     4081 agttgttcaa gtttattgct actatttttg atggtatgaa tgtgcttata atgtatcccg
     4141 gttaacgaaa gttttggact tatactctta gaaaatctct tcaaaccacg tcaacgtcgc
     4201 cttgccgtgc gtatggttac tgactacgtc agttctatcc acaacctcaa aaccgtgttt
     4261 tgagcaacct gcgactagtt tcctagtttg ctctttgatt ttcattgagt actaatctta
     4321 ttttttaacg tgtgctaaaa aataaattaa tgcatgttaa tttttcttga attaaatttc
     4381 tttaaatgat atactgtttt tatggagaga taacaattat atataaatgt actactctat
     4441 ttcctagata atacttagta aaacttttta taacaaaggc tagcaaagtt aaagtttatt
     4501 atctattgga agttaaataa atatgtaagg aattaaaatg aaaaaaagta cagtattgtc
     4561 attaaccaca gctgcagtta ttttagcagc ctatgcccct aatgaggtag tcttagcaga
     4621 cacatctaac tctgaagatg ctttaaacat ctctgataaa gaaaaagtag cagaaaataa
     4681 agagaaacat gaagatatcc atagtgctat ggaaacttca caggatactg aagaaaagaa
     4741 aacaacaatt attgaggaaa aaaaagttgt tagtaaaaat cctgtgatag acactaaaac
     4801 tagcaatgaa gaagcaaaaa tcaaagaaga aaattccaat aaatcccaag gagatcatac
     4861 ggactcattt gtgaataaaa acacagaaaa tcccaaaaaa gaagataaag ttgtctatat
     4921 tgctgaattt aaagataaag aatctggagc aaaagcaatc aaggaactat ccagtcttaa
     4981 gaatacaaaa gttttatata catatgatag aatttttaac ggtagtgcca tagaaacaac
     5041 tccagataac ttggacaaaa ttaaacaaat agaaggtatt acatcggttg aaagggcaca
     5101 aaaagtccaa cccatgatga atcatgccag aaaggaaatt ggagttgagg aagctattga
     5161 ttacctaaag tctatcaatg ctccgtttgg gaaaaatttt gatggtagag gtatggtcat
     5221 ttcaaatatc gatactggaa cagattatag acataaggct atgagaatcg atgatgatgc
     5281 caaagcctca atgagattta aaaaagaaga cttaaaagga actgataaaa attattggtt
     5341 gagtgataaa atccctcatg cgttcaatta ttataatggt ggcaaaatca ctgtagaaaa
     5401 atatgatgat ggaagggatt attttgaccc acatgggatg catattgcag ggattcttgc
     5461 tggaaatgat actgaacaag acatcaaaaa ctttaacggc atagatggaa ttgcacctaa
     5521 tgcacaaatt ttctcttaca aaatgtattc tgacgcagga tctgggtttg cgggtgatga
     5581 aacaatgttt catgctattg aagattctat caaacacaac gttgatgttg tttcggtatc
     5641 atctggtttt acaggaacag gtcttgtagg tgagaaatat tggcaagcta ttagggcatt
     5701 aagaaaagca ggcattccaa tggttgtcgc tacgggtaac tatgcgactt ctgcttcaag
     5761 ttcttcatgg gatttagtag caaataatca tctgaaaatg accgacactg gaaatgtaac
     5821 acgaactgca gcacatgagg atgcgatagc ggtcgcttct gctaaaaatc aaacagttga
     5881 gtttgataaa gttaacatag gtggagaaag ttttaaatac agaaatatag gggccttttt
     5941 cgataagaat aaaatcacaa caaatgaaga tggaacaaaa gctcctagta aattaaaatt
     6001 tgtatatata ggcaaggggc aagaccaaga tttgataggt ttggatctta ggggcaaaat
     6061 tgcagtaatg gatagaattt atacaaagga tttaaaaaat gcttttaaaa aagctatgga
     6121 taagggtgca cgcgccatta tggttgtaaa tactgtaaat tactacaata gagataattg
     6181 gacagagctt ccagctatgg gatatgaagc ggatgaaggt actaaaagtc aagtgttttc
     6241 aatttcagga gatgatggtg taaagctatg gaacatgatt aatcctaata aaaaaactga
     6301 agtcaaaaga aataataaag aagattttaa agataaattg gagcaatact atccaattga
     6361 tatggaaagt tttaattcca acaaaccgaa tgtaggtgac gaaaaagaga ttgactttaa
     6421 gtttgcacct gacacagaca aagaactcta taaagaagat atcatcgttc cagcaggatc
     6481 tacatcttgg gggccaagaa tagatttact tttaaaaccc gatgtttcag cacctggtaa
     6541 aaatattaaa tccacgctta atgttattaa tggcaaatca acttatggct atatgtcagg
     6601 aactagtatg gcgactccaa tcgtggcagc ttctactgtt ttgattagac cgaaattaaa
     6661 ggaaatgctt gaaagacctg tattgaaaaa tcttaaggga gatgacaaaa tagatcttac
     6721 aagtcttaca aaaattgccc tacaaaatac tgcgcgacct atgatggatg caacttcttg
     6781 gaaagaaaaa agtcaatact ttgcatcacc tagacaacag ggagcaggcc taattaatgt
     6841 ggccaatgct ttgagaaatg aagttgtagc aactttcaaa aacactgatt ctaaaggttt
     6901 ggtaaactca tatggttcca tttctcttaa agaaataaaa ggtgataaaa aatactttac
     6961 aatcaagctt cacaatacat caaacagacc tttgactttt aaagtttcag catcagcgat
     7021 aactacagat tctttaactg acagattaaa acttgatgaa acatataaag atgaaaaatc
     7081 tccagatggt aagcaaattg ttccagaaat tcacccagaa aaagtcaaag gagcaaatat
     7141 cacatttgag catgatactt tcactatagg cgcaaattct agctttgatt tgaatgcggt
     7201 tataaatgtt ggagaggcca aaaacaaaaa taaatttgta gaatcattta ttcattttga
     7261 gtcagtggaa gaaatggaag ctctaaactc caacgggaag aaaataaact tccaaccttc
     7321 tttgtcgatg cctctaatgg gatttgctgg gaattggaac cacgaaccaa tccttgataa
     7381 atgggcttgg gaagaagggt caagatcaaa aacactggga ggttatgatg atgatggtaa
     7441 accgaaaatt ccaggaacct taaataaggg aattggtgga gaacatggta tagataaatt
     7501 taatccagca ggagttatac aaaatagaaa agataaaaat acaacatccc tggatcaaaa
     7561 tccagaatta tttgctttca ataaccaagg gatcaacgct ccatcatcaa gtggttctaa
     7621 gattgctaac atttatcctt tagattcaaa tggaaatcct caagatgctc aacttgaaag
     7681 aggattaaca ccttctccac ttgtattaag aagtgcagaa gaaggattga tttcaatagt
     7741 aaatacaaat aaagagggag aaaatcaaag agacttaaaa gtcatttcga gagaacactt
     7801 tattagagga attttaaatt ctaaaagcaa tgatgcaaag ggaatcaaat catctaaact
     7861 aaaagtttgg ggtgacttga agtgggatgg actcatctat aatcctagag gtagagaaga
     7921 aaatgcacca gaaagtaagg ataatcaaga tcctgctact aagataagag gtcaatttga
     7981 accgattgcg gaaggtcaat atttctataa atttaaatat agattaacta aagattaccc
     8041 atggcaggtt tcctatattc ctgtaaaaat tgataacacc gcccctaaga ttgtttcggt
     8101 tgatttttca aatcctgaaa aaattaagtt gattacaaag gatacttatc ataaggtaaa
     8161 agatcagtat aagaatgaaa cgctatttgc gagagatcaa aaagaacatc ctgaaaaatt
     8221 tgacgagatt gcgaacgaag tttggtatgc tggcgccgct cttgttaatg aagatggaga
     8281 ggttgaaaaa aatcttgaag taacttacgc aggtgagggt caaggaagaa atagaaaact
     8341 tgacaaagac ggaaatacca tttatgaaat taaaggtgcg ggagatttaa ggggaaaaat
     8401 cattgaagtc attgcattag atggttctag caatttcaca aagattcata gaattaaatt
     8461 tgctaatcag gctgatgaaa aggggatgat ttcctattat ctagtagatc ctgatcaaga
     8521 ttcatctaaa tatcaaaagc ttggcgagat tgcagaatct aaatttaaaa atttaggaaa
     8581 tggaaaagag ggtagtctaa aaaaagatac aactggggta gaacatcatc atcaagaaaa
     8641 tgaagagtct attaaagaaa aatctagttt tactattgat agaaatattt caacaattag
     8701 agactttgaa aataaagacc taaagaaact cattaaaaag aaatataagg aagaagacga
     8761 ctttgtaact ggtggaaaaa gaacagtaga acttgattat aaatatgacg ataaaggaaa
     8821 tataactgcc tatgaagatg aaagtgctct agaatatgaa actgaaaaat ttgacgaaat
     8881 aaaatcaaaa atttatggtg ttctaagtcc atctaaagat ggacactttg aaattcttgg
     8941 aaagataagc aatgtttcta aaaatgccaa ggtatattat ggaaataact ataaatttat
     9001 agaaatcaaa gcgactaagt atgactcaca ttcaaaaacg atgatatttg atttatacgc
     9061 taatattaat gatattgtag atggattagc ttttgctgga gatatgagat tatttgttaa
     9121 agatgacaat caaataaaag ctgaaactaa aattagaatg cctgaaaaaa ataaggaaac
     9181 taaagcagaa tatccctatg tatcaagtta tgggaatgta atcgaattag gagaaggaga
     9241 cctttcaaaa aacaaaccag acaatttaac taaaatggaa tctggtaaaa tctattctga
     9301 ttcagaaaaa caacaatatc tgttaaagga taacatcatt ctaagaaaag gctatgcact
     9361 aaaagtgact acctataatc ctggaaaaac ggatatgtta gaaggaaatg gagtctatag
     9421 caaggaagat atagcaaaaa tacaaaaggc caatcctaat ctaagagtcc tttcagaaac
     9481 aacaatttat gctgatagta gaaatgttga agatggaaga agtacccaag ctgtattaat
     9541 gtcggctttg gacggcttta acattataag gtatcaagtg tttacattta aaatgaacga
     9601 taaaggggaa gctatcgata aagacggaaa tcttgtgaca gattcttcta aacttgtatt
     9661 atttggtaag gatgataaag aatacactgg agaggataag tccaatgtag aagctataaa
     9721 agaagatggc tccatgttat ttattgatac caaaccagta aacctttcaa tggataagaa
     9781 ctactttaat ccatctaaat ctaataaaat ttatgtacga aatccagaat tttatttaag
     9841 aggtaagatt tctgataagg gtggttttaa ctgggaattg agagttaatg aatcggttgt
     9901 agataattat ttaatctacg gagatttaca cattgataac actagagatt ttaatattaa
     9961 gctgaatgtt aaagacggtg acatcatgga ctggggaatg aaagactata aagcaaacgg
    10021 atttccagat aaggtaacag atatggatgg aaatgtttat cttcaaactg gctatagcga
    10081 tttgaatgct aaagcagttg gagtccacta tcagttttta tatgataatg ttaaacccga
    10141 agtaaacatt gatcctaagg gaaatactag tatcgaatat gctgatggaa aatctgtagt
    10201 ctttaacatc aatgataaaa gaaataatgg attcgatggt gagattcaag aacaacatat
    10261 ttatgtaaat ggaaaagaat atacatcatt tgatgatatt aaacaaataa cagacaagac
    10321 actaaacatt aagattgttg taaaagattt tgcaagaaat acaaccgtaa aagaattcat
    10381 tttaaataaa gatacgggag aggtaagtga attaaaacct catagggtaa ctgtgaccat
    10441 tcaaaatgga aaagaaatga gttcaacgat agtgtcggaa gaagatttta ttttacctgt
    10501 ttataagggt gaattagaaa aaggatacca atttgatggt tgggaaattt ctggtttcga
    10561 aggtaaaaaa gacgctggct atgttattaa tctatcaaaa gataccttta taaaacctgt
    10621 attcaagaaa atagaggaga aaaaggagga agaaaataaa cctacttttg atgtatcgaa
    10681 aaagaaagat aacccacaag taaaccatag tcaattaaat gaaagtcaca gaaaagagga
    10741 tttacaaaga gaagatcatt cacaaaaatc tgattcaact aaggatgtta cagctacagt
    10801 tcttgataaa aacaatatca gtagtaaatc aactactaac aatcctaata agttgccaaa
    10861 aactggaaca gcaagcggag cccagacact attagctgcc ggaataatgt ttatagtagg
    10921 aatttttctt ggattgaaga aaaaaaatca agattaagac aaaagctata gaaaaaaatg
    10981 gtttatgtac tgagattaga tagtgaggtg atgacatagt tttgtgaaaa cagccattta
    11041 taactcaatt atttagttta cttcactatt gatactattt ggagttatta atggacttag
    11101 tttagataac taatgaattg attgaaaggg ttagtattga caatattggt catattgatt
    11161 agaaaataga gtctatcaaa atttaaaggc taatagaggt gatgagacaa tttcaaggaa
    11221 ttaagtagcg tttggttgct taattccttt ttatttggaa ggaagttttt cgtaaccgcc
    11281 caataacgaa gtctattgga cggttacgat atgatcactg acttcgtcag tcttatctac
    11341 aacctcaaaa ctgtgttttg agcaacctgc ggctagcttc ctagttt
//
LOCUS       DBERMR010000066        10755 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.066, whole genome shotgun sequence.
ACCESSION   DBERMR010000066 DBERMR010000000
VERSION     DBERMR010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10755
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.066"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(117..1541)
                     /locus_tag="ACL8A1_001904"
     CDS             complement(117..1541)
                     /locus_tag="ACL8A1_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HGS1202613.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAIERAAYLHKTAAILERDKEKIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2086..4365)
                     /gene="pulA"
                     /locus_tag="ACL8A1_001905"
     CDS             complement(2086..4365)
                     /gene="pulA"
                     /locus_tag="ACL8A1_001905"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HGS1202614.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGTYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGCLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4477..6435)
                     /gene="ligA"
                     /locus_tag="ACL8A1_001906"
     CDS             complement(4477..6435)
                     /gene="ligA"
                     /locus_tag="ACL8A1_001906"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HGS1202615.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQARQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLYQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6528..7706)
                     /locus_tag="ACL8A1_001907"
     CDS             complement(6528..7706)
                     /locus_tag="ACL8A1_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGS1202616.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7786..8649)
                     /locus_tag="ACL8A1_001908"
     CDS             complement(7786..8649)
                     /locus_tag="ACL8A1_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HGS1202617.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTPLYFELNWILLQGLICQRDATYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKDIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAIHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8652..10553)
                     /locus_tag="ACL8A1_001909"
     CDS             complement(8652..10553)
                     /locus_tag="ACL8A1_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HGS1202618.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNNLRRTERQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETIAWLENYLVNYSGALLIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLVTEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYIEKRATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSEQV"
BASE COUNT         3226 a         2369 c         1914 g         3246 t
ORIGIN      
        1 tgtactattc tagtttcaat atactataat accaaaaagt tcgtattttt tctataatta
       61 ttattatttt atagcaaaaa aataaaacca ggaaaacaat ttcctgattt tacacgttat
      121 ttcacatcaa aaacaatgga tttgacattt gtcatcgctt caatgctata tttaatccct
      181 tgcactccag ctccagaacc tttgacacca aggaacggga aattatctgg accacgctgg
      241 gttttattat taatgtggac tgtacctact tcaagttttt cagcaatttc aaaggctttt
      301 ttgaaatcat ttgtaaagac tgatgattga aggccgaatt cagattcgtt ggcaaaggca
      361 atagcttcct ctacactagc cacacgaatg attggtaaaa caggaccaaa tggctcttcc
      421 catgccactt tcatatcttt tgtaacttgg tcaaaaagca ctggccagag aagattgccc
      481 tcacgtttga ttggtgtaag agcctgagcc tctttttctt gtgcatcctc aatcaagccc
      541 caaatgaagt cggctgaagc attgtcaata acaggtgtaa tatcagcatt gtcaaatgga
      601 tcaccaactg ttaatttaga aacttcttcc tgaagcaaag tagctaattt atctgctaca
      661 ctttcgagaa caatgacacg tttaatggcc gtgcaacgtt gtcctgagta gctaaaggct
      721 cctgcaacaa tttgcttggc agcatgttcc aaatctgcat cttctagtac aagagctgca
      781 tctttcccac caagttccaa catgatagga cgcataccag ctaaacgacc aatacgttct
      841 ccaatagggg ttgaacctgt aaagttgatg aagttgactt ctttgtgctc aatgatataa
      901 tccccaattt ctgaaccacg acctgtaatg gtgttgaaaa cacctgccgg aatccctgct
      961 tcttcaaatg ctttagccaa caagagtcca gaaatggaac cttgtgttgg tggcttaaac
     1021 atgaccacat tccctgcaat caaggcaggt gcaattttag aagcagataa attaactgga
     1081 taattaaagg gagcaatcgc tagcacgata ccaactggtt cacgacggac aacagccagt
     1141 ttgtttttac ttgctgcctc aaaaccacca ccttccattg cttgtccagt gatacggaga
     1201 ccttcctcag cagcataacg aatcaagtct gctgtacgca ctacttctcc aattgctgct
     1261 ttaatccctt ttgctacttc tttggcaagg atagtaccaa ttttttcctt atcgcgttct
     1321 aaaatagctg ctgttttatg caaataagcc gcacgttcaa ttgctgataa agctcgccat
     1381 gctggcaggg ctgcacgcgc agcttgcata gcctcatcag cttcagtctg agtcatggct
     1441 ggaactgtac ccaattcttc ttgattgatt ggtgaataaa tcgtaatttc ttgttcagat
     1501 gatttccatt ttccatttac taaattctga tatcttgtca aattcttgtc ctcctgaaaa
     1561 tgattaagat atattttacc aaattttcag aaaatcacaa ccctttttgc aaaatttttg
     1621 agattatttt cacaagcttg atttttcaaa gtatactcaa taaaaataaa aaaaatccac
     1681 tacatcaagg cgagactaat gtggtttgaa gaaattttcg aagagcgtga atgagtatca
     1741 tctatagtaa aataaaaaaa ctgaacaatt tggttgggga cagccaaacc aatttctcac
     1801 aatgtttcag aaacaagggt gtgctattcc aatttcagcc tactataact gtcatagatt
     1861 gctgaaacaa agtctaggta aaagtcttca taataaaaag acctcctatc aaatgttcaa
     1921 aaactttgat aggaggtctt gtttcgtgaa aacatttatc aaattttcta tacaagtgag
     1981 ctgttagcca ggttctttct attctttcaa tttcaatgaa tggatttttt accaatactc
     2041 ataactggga atttttctgt ctaaaaatag cgagatagat ggtatttata aaacactcaa
     2101 gacagctaga ctaatatcat ttaaaacatt atcttctttt gagcgactgt tggttaccaa
     2161 catagctaaa tttcctgcat tttcaaattg atagggttct gatttagcat tcacaaccac
     2221 caagaggtgt tctttgccgt gaacttcata gataaggcag ccgctatgtt caatcgcaga
     2281 atgcacaaag acatgatggt aaatttcatc atagctagag taagaaaagg caccagtttt
     2341 tgtcttcaat cggatgactt gacggataaa ctcaatactg tcttgacgct cattaatcaa
     2401 gttccagttc acttggttca cactgtcagg agcattatag ctattcatcg cacgctctct
     2461 atcatcatgg gtcaactcac cattttcacc agtcgcaacc agtttggtac gaccaaattc
     2521 ttgaccgatt tccataaagg ccatcccctg catgagcaga ttcatggctg tggcagtttc
     2581 gaccttgcgc atgatttgct ctgaactttg gtctggatga agggttgcca ataaatcgtg
     2641 aagattgtaa ttgtcatggg cttctacata gttaagcacc tgatttggat gtgtataggt
     2701 tcctaattca cgacttccta ggattgcttt agctagaatt ggctctgtcg cagcaccact
     2761 gacaaaacct gacttgatag caccataaac ttctccccct ttgacagcat cgcgctgatt
     2821 gtcattaaag aaaccaatat ttggcatctg gtaggcattg tccttcttgg ccttatcata
     2881 aggggcaaga cctgttccca tatcccatcc ttctccatag aggataatgt tggagtcgat
     2941 ttcatccaag ctttgacgaa tcatctgcat ggtcttgaca tcatgaatcc ccatcaagtc
     3001 aaaacggaag ccgtcaatat tatattcctg cacccagtat agaagagaat caatcatata
     3061 cttgcgaaac atttcgtgtt cactggctgt ttcatttcca acacccgttc cattctggaa
     3121 ggtaccatct ggattcatac gataatagta atcagggact gttgtttgga atggtgcatc
     3181 aacaactgag aaggtatggt tatagactac atccataatg actccaatac ccgcatcgtg
     3241 ataagcttga accatcacct tcaaatcacg aatgacctga gctggatcat ctggattagt
     3301 tgaaaaacta gtttctggcg cgttatagtt ttgtggatca taaccccagt tgtaggttac
     3361 atttccatcc tcatcgtatt ctttatgacg gtctgcaatt ggttgcaatt gaacataatt
     3421 gtagcccagc ttcttgatgt aatcaaaagc agttgactgg ccgtattggt taactgttcc
     3481 agcctgagca gcacccaaga aagttcctcg aagatgttca tctacacccg atgtaggtga
     3541 tttagtcaaa tcacgaatgt gcatttcaca gataactgcc ttacatggat tttccaagcg
     3601 ccaagtagcc tccgaaccgt gcttaacctc gaagttttca acttgctttt ctacatggct
     3661 cagaatagct gaacgtttgc catcagggct ggtcgcgatt gtataaggat cacgtgtcag
     3721 tgtttggtga tgagggaatt ggacttgata ctgataagtc ttacctacca aatcttcttc
     3781 aacatccaaa ctccagacac cgattgtatt gtccttatga ttataagagt agctattgcc
     3841 tcttttcatc tcaaaagtct tccaaacggg tgcatcatta gcagctgatt cataaacgac
     3901 aacttgtact tctgtcgctg taggtgacca gagggaaaaa tgagcctgat tgtcctctac
     3961 acggcaaccc aattctcctt ggtaacccca atgatgatca aaactagcac tgttaatggc
     4021 cttatcaaag gcaaaaggat tttgattttt atagaaaggg ctggcaatag caggattttc
     4081 agagtaataa atcctatcat cgccttccaa aatccagacc tctgttaata ggggatagtg
     4141 attaaaacgg atagaatatt ctttactagt ttgacctgta tgaaccacaa aattcaagct
     4201 ttctataaca tgtgaacttg ggtgttcaaa gctaaataaa gctccaaaat aatcttcttt
     4261 gtaggttagc aaatcaattc gttgatcctg actttttaca aaggagcaag tgtcatattc
     4321 tccattctta cgatggtaat gaatgcgcat agggtagtta tacatttttt atttttcctt
     4381 tttactttgt ttctatttca ctaataaatt tttgtcaatc tcgtctcaat taacagacat
     4441 agtcatattc tctaaactct gtttttaaac gatccattac aaactttcta gccatgcctc
     4501 atctctgacc tggataccaa gttcttgtgc tttttgcagt ttacttccag cgtctgcacc
     4561 taccacgacg aggtcggtct ttttagaaac actacctgtc actttggcac ccagactttc
     4621 gagtttactt ttagcttctg agcgcttgag tcgttccaat tttcctgtca ataccacggt
     4681 caaacctgac aaggccgcat ccgctactac cgtctgtcct ttatagtcca gattgacccc
     4741 agtttctttc aattctctga gcagaatttc agagccttct gtcgcaaaat aagtctgaag
     4801 acttttggca atcacgccac ctagactttc aatactggcc acttcctctg aatctgcctg
     4861 atacagattt tcaattgaat gaaaatattg aagtaaaagc tgactagcct tgcttccgac
     4921 atgacgaatt cccaaaccaa ataagagctt ctcggcagaa ttttcctttg atgcttggat
     4981 agcctgatac agtttagcag cggacttttc cttaaccccc tctaaaagga ggaaatcctc
     5041 ttcttgcaaa cgataaatat ccgccacatc cttgactaaa ttagcagcaa aaagcttctc
     5101 aacaatagat ggaccaaggc ctgtaatatt catagcatca cgagaagcaa agtgaatcaa
     5161 gccttccatg atttgagcag ggcaacgcgg attgatacaa cgtagggcca cttcatcttc
     5221 aaagtgcaac aagtcagagt tacaacttgg acagtttgta gggatatcta gtttttcttc
     5281 agaaacccgt ttggactcta ccacacgtaa aacggcaggg atgatgtcac cagccttata
     5341 tacaatgacc gtatcgtctt ttcggatatc tttttcagca atataatcta cattgtgcag
     5401 ggtcgcacgg ctaacagtcg taccggcaag ttgtactggt gttagattag cagttggagt
     5461 tacaacaccg gtacggccaa ctgtccagtc aactgataag agttgagctt ctttttcttc
     5521 ggcagggaac ttgtaggcta ctgcccactt tggagcctta actgtaaaac caagttcttc
     5581 ttgacttgct aggtcgttga ccttgattac cactccatca atatcgtaag gcagattttc
     5641 ccgttcctgt cctacttctt ggataaaatt ccagatttca tctatgtttt cagccaagat
     5701 tcgcttagga ttgaccacaa aacctagttg ttctaggtac ttcaaaccct tttcttggct
     5761 atcacgagtt gaagggctgg cttcttgata gagaaacgtt gcaagattac gcttggcaac
     5821 tactgctgta tccaactgac gcagagttcc tgctgccgca ttacgaggat tagcaaattc
     5881 aggctctcca ttttcttggc gcgcttggtt aacttggtca aaggaagcgc gtggcatgta
     5941 acattcccca cgaactgtga tatctagttc ttctggcaaa gtcaaaggga tgtccttaac
     6001 acgcttgagg ttttctgtga tattttcacc aattgaacca tctccacgtg ttaccccagc
     6061 aaccaaaatc cccttttcat aagtcagcga gatagataag ccatcgattt tcagctcaca
     6121 aatataggtc ggatgagcca cttccttacg aacacgcgca tcaaaagcat ctagctcctc
     6181 acgtgaaaaa gcatcctgca aactataaag aggatactga tgactgtatt tttcaaaacc
     6241 atctaaaacc ttgccaccaa cacgatgagt cggactgtct gctagcactt gctctggata
     6301 agcagtttct aactcgacca actcacggta aaggcggtca tactcactgt ctgaaaccga
     6361 gggattatcg ctggtatagt actcagtcgc atagcgattg agcaaagcga ctaactcatt
     6421 cattctttta ttcataagac cattttacca taaaacaagc cctcctcaca aacgagaagg
     6481 gcggaaaaaa cacttagttt gaaattattt ttgaaactca agcaacctta tatcaatttt
     6541 tcaaaatgag ttcgaacata aataaacgat atacaagaca agatgataac accacttcca
     6601 attatcagga aagaagagag atgtacactt ggcaagactg tcataaatcc ttttgcaata
     6661 ggcataaata gaatagctaa ggtaaaaatt gtactcagta ctctcccaag aaattcgctc
     6721 tcaaccttgg tttgtacttg agtaaaaaag tgaatattaa aaatcgtcat aaacaattca
     6781 caaactaaat ttccagaaaa ggaaagaaaa gttggaagtg gtaatcccat cataaaaact
     6841 ccgacacctg tcaaagccag taaaatcaaa agattataaa tattagcttt aattttacta
     6901 gctagaagag ccccaatgat ggaaccaata gcccccatag ttaaaatact tgcataggct
     6961 ccttctgacc cgtaaagctg attcgaaaag ggaagtagaa attcaaaagc tgcaaaaaag
     7021 aaattaacgc tggaagctac cagcaaaagg aagaaaattt cttgctgatg ccagatatag
     7081 tgtaacccat ccttgatatc tacaaaaata tctctcccag taaaagcctt tttctcttga
     7141 acttttgctt cctcttttgg aaggaaagcc actagaacaa aagcaatgaa aaaagtcagc
     7201 gagtctagca gtagcgtcat atggagactt gcaaactgta aaacaaggaa ggaaagaaca
     7261 ggagagctaa cacctacaac ctgcaaaacc agctctaagc gagaattata gatcacaatc
     7321 tcatctttct ccaccacttc agttatgata gctttattgg ctgtgcgaga aaaggcaaaa
     7381 gcaatagcct gcacaatgtt agcaacaatc aaagcgccaa tcatccagct atcattcctt
     7441 atgaaagaaa tagccagaca aagaatccca caaacaagat ctgccgtcat taaaatctta
     7501 cgacgagaaa aacggtctga aataactccg ccaaagggat tgacgagaat agatgtgacg
     7561 agctcagaaa tctgatacat tcctaaaact gtctgtccta tagtccccat agaagccaac
     7621 cagacactat ttccataatc atagagcata tttcccattt tattgatagc cccacggcta
     7681 atcaactgca ctgcatagcg attcatatta aagctcctct caaattttga aactattgta
     7741 tcaaaaccga aaggagcttt ttattttttc ccttatttgg gaaaattaac ttttgacaaa
     7801 tttttcgtag tgttcctgat aataggctac ttgctctgga agacctaaca catcaaaaat
     7861 atgtatggcc tcttgcatct gcttacagcc ttctttacac tgtccttttt gatataaggc
     7921 aaaacctttt aaataatgga aaacattacg ctcataaagc ttaatatctt tgtcaataat
     7981 cttctctgta taagcctcaa aatagttggc attataaaaa gaagaatgct ctaaacaatg
     8041 ctggtaacaa ttgagggcca aaattaacac taatctctta tggcgactaa tctcttggta
     8101 aaattcctcc ctctccataa cttctctacc aatccgagtg acatagtcta catcgtagaa
     8161 actatagagg ttaccgaaaa gaatcaactc atacatggtc cattcttctg ttttgaagag
     8221 ataatctgct accttatcca aatcatcctg cttcatatca taagtcgcat ctctttgaca
     8281 aatcagacct tgtagcaaaa tccagttcag ctcaaaataa aggggagtcg tcgaactctt
     8341 agacttttca agttgttctc tttgaagctt ttgaaaacct gcaatatcgt ttgaatagta
     8401 aagtgggata atctgtgcca tcatagacac atgttcatga ttatgaaaat tccttgcctt
     8461 atccatgaaa ttttcgattg ttacatgaat gttatccaaa atctcaaaga aacgggagac
     8521 tgccaggtca gactccccaa gctcaaagcg agataactga gaggtagagc aggattcgcc
     8581 tgctgcttcc tttaaagaat aatttccact tgttcgaaat tcacgaaata cttttccaag
     8641 atgttccatt tttacacctg ctctgataat tcttcccact caagcatagc ttcttcctga
     8701 cgatggctga ttttgtccag ctcagcctgt aattccatga gtttgtcggc atcgtttgtt
     8761 tccaacattt gttcagaaat ggcttggctt tgactttcta gctcttcaat ttcagcttct
     8821 agactttcga tttgtcgcat gagtttgcga acttcttttt gactttcttt ctgggcctga
     8881 tagtcattga ctggacttgc ttcctttgct tgattgctag ttgaagcttc ctcagtctga
     8941 ctcatttctg ctgttgctct cttctcaata tagtagtcgt aatctccaag gtagagagtt
     9001 gaaccattct cagacaattc caaaacatga gttgccacac gattgataaa gtaacgatca
     9061 tgactgacaa acagcaaggt tccatcaaag tcaatcaagg cattttctag cacttcctta
     9121 ctatcaatat ccaagtggtt ggtcggctca tccagaatca aaaaattatt gttttccata
     9181 gacaatttag ctaaaagcaa acgagctttt tcgccaccag atagcatgcc gactgatttt
     9241 ttaacatcat ctcctgagaa aaggaaggct ccaagacggt tgcggatttc aacttctggt
     9301 gtcagtttga aatcattcca gagttcatcc agcaccgtat tacttggtgt cagcttgctt
     9361 tgggtttggt catagtaacc aacctcaaca ttagcgccaa agcgcttttc tcccttgata
     9421 aaaggaatct ggtccacaat agacttgata aaggttgact tgccgatacc atttggacca
     9481 acgatagcga cagcattcat cttacgaaga tctaggttga taggttgtga caagacttcc
     9541 ccgtcataac caatagcagt attttcaaca gtcaaaacaa cattgcccga cgttttttca
     9601 gactggaagg tcatgttggc tgctttcttg ccagcttcag gcttgtccaa acgctccatt
     9661 ttttctagtt gtttacggcg agattgagca cgtttagtcg ttgaagcacg aactagattg
     9721 cgattgacaa agtcttccag agcagcgatt tccttctgtt gcttttcata gttttttgcc
     9781 tcagtaacta gcttttgctc cttcaattcg acaaaacgag agtaattccc cacatagcga
     9841 tccaaggaat gcttggtcaa atctagcgta attgtcgcaa ccttgtccaa gaaataacgg
     9901 tcgtggctga cgataaggag ggcaccgcta tagtttacca agtaattctc tagccaggcg
     9961 atggtttcaa tatccaagtg gttagttggc tcgtccaaga ccaagagatt gggcttttca
    10021 aggagcattt tggcaagtgc caaacgagta ttttgaccac cagaaagctc agcaattttc
    10081 atctgccaca tagactcgtc aaacttgaat ccattcaaaa tcgctcgaat atcagcttca
    10141 taggtaaagc cacctgcttg gcgaaaattc tcagataagc ggtcataatc tgacatcagt
    10201 ttatccaaat cctcaccaga cttttcaccc atctccagct ccatctgacg cagttgtctc
    10261 tccgtccgac gcaaattatt aaagacatga agcatttcat cgtagatggt attttcagac
    10321 tcaaaacggc tatcttgggc taggtaagac agagaaatat cttttttctt attgatttct
    10381 ccgctagttg gctcctcttc tccaactaaa atcttcaaaa gagtagactt acctgcacca
    10441 tttttcccaa caagggcaat ccgatctcgt tcatcaacct gcaggttgat attatcgaaa
    10501 agaacctctc ctgcaaaaga acgttcaatt ttattagctt gtaaaataat cataagagta
    10561 gtatagcatg tttccctaag gcattcaaga taatcgtaag tcttttagta caacttttat
    10621 aacataaaat aaactaaatt atgtatattt tatattagat tcttcactat cttgttggat
    10681 tttctaacca gctaatcttg tttcaaatag ttatcgcaca agcctattat ttaattcttt
    10741 tcattattta cgtac
//
LOCUS       DBERMR010000067        10749 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.067, whole genome shotgun sequence.
ACCESSION   DBERMR010000067 DBERMR010000000
VERSION     DBERMR010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10749
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.067"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            55..279
                     /locus_tag="ACL8A1_001910"
     CDS             55..279
                     /locus_tag="ACL8A1_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HGS1202619.1"
                     /translation="MIAGGLIGLLAGKITKKSSSMGIIANVFAGLVGASAGQSLLGSW
                     GSSIAGMALLPSIVGAAIVITVVSFFTGRK"
     gene            363..929
                     /gene="amaP"
                     /locus_tag="ACL8A1_001911"
     CDS             363..929
                     /gene="amaP"
                     /locus_tag="ACL8A1_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HGS1202620.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            941..1111
                     /locus_tag="ACL8A1_001912"
     CDS             941..1111
                     /locus_tag="ACL8A1_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HGS1202621.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            1150..1758
                     /locus_tag="ACL8A1_001913"
     CDS             1150..1758
                     /locus_tag="ACL8A1_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HGS1202622.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            1789..1992
                     /locus_tag="ACL8A1_001914"
     CDS             1789..1992
                     /locus_tag="ACL8A1_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HGS1202623.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(2087..2326)
                     /locus_tag="ACL8A1_001915"
     CDS             complement(2087..2326)
                     /locus_tag="ACL8A1_001915"
                     /inference="COORDINATES: protein motif:HMM:NF025126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGS1202624.1"
                     /translation="MNERYQCLKTKEYQALLSSKGRQIFAKRKIDMKSVFGQIKVCLG
                     YKRCHLRGKRQVRIDMGFILMANNLLKYNKRKRQN"
     gene            2605..2850
                     /locus_tag="ACL8A1_001916"
     CDS             2605..2850
                     /locus_tag="ACL8A1_001916"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HGS1202625.1"
                     /translation="MAESIQGWLAQFLVNLFKSITFDCGKEFSKWKDISNHHDSESFF
                     ANLGCPRQRCLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            complement(3128..3898)
                     /locus_tag="ACL8A1_001917"
     CDS             complement(3128..3898)
                     /locus_tag="ACL8A1_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HGS1202626.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKDATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPIPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(3913..4761)
                     /locus_tag="ACL8A1_001918"
     CDS             complement(3913..4761)
                     /locus_tag="ACL8A1_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HGS1202627.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIADTPV
                     TNQEFVQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGAPWWLQVSNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDGFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(4758..6233)
                     /locus_tag="ACL8A1_001919"
     CDS             complement(4758..6233)
                     /locus_tag="ACL8A1_001919"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HGS1202628.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYASDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTALKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            complement(6556..7911)
                     /locus_tag="ACL8A1_001920"
     CDS             complement(6556..7911)
                     /locus_tag="ACL8A1_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGS1202629.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     NVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMAYKTEL
                     S"
     gene            complement(7921..8238)
                     /locus_tag="ACL8A1_001921"
     CDS             complement(7921..8238)
                     /locus_tag="ACL8A1_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGS1202630.1"
                     /translation="MNESNLESVMGLIMYGGEAKSNAMEAIQAAKKGDFSKANKKLAD
                     ANAALLKAHMAQTEMLTKEAQGEETSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(8255..8572)
                     /locus_tag="ACL8A1_001922"
     CDS             complement(8255..8572)
                     /locus_tag="ACL8A1_001922"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS1202631.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(8587..9483)
                     /locus_tag="ACL8A1_001923"
     CDS             complement(8587..9483)
                     /locus_tag="ACL8A1_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HGS1202632.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLKRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(9486..10748)
                     /locus_tag="ACL8A1_001924"
     CDS             complement(9486..10748)
                     /locus_tag="ACL8A1_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGS1202633.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFIRGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDILLYLPFVIAIEKRFKLLED"
BASE COUNT         3703 a         2172 c         1665 g         3209 t
ORIGIN      
        1 aaattgattt tttgatatta aaataaaaaa ggagggtagg cagtgttgtg atcaattatt
       61 gctggaggtc ttattggtct cttggcaggt aaaatcacta aaaaaagtag ttctatggga
      121 atcatcgcaa atgtattcgc tggtttagtc ggggcatctg caggacaatc tcttttaggt
      181 agttggggtt catcaatcgc tggaatggct ttgctcccat ctattgtagg tgcagcgatt
      241 gtgattactg tagtgtcatt ctttacaggt agaaagtaaa cttttcgcca gtaaagttag
      301 caaactattt ttaaatcaat gacgggaaaa atagtttaaa tgttaaatcg aaaggattgt
      361 atatgtcaaa agcaaagaaa atatgtttca ttattttctg tattttaatc ttgacaattt
      421 tccttcctgt tttgatagat tatcatcaag ttagtgatct aggtattcat ctacttagct
      481 ggagacagaa ctccgtagtt gaattctatc ttgctagata tgtcttttgg gggacagtgg
      541 ttctatcaac tttagtttta ttatccattt tagttgtgat gttttatcct aaacgttact
      601 tggaaatcca acttgaaact aaaaacgata cattaaaatt aaagaattcg gcaatcgaag
      661 gttttgttag aagtttggtg agtgatcata gattgatcaa gaacccaact gttcatgtaa
      721 atttacgaaa aaataaatgt ttcgttcatg tagaaggtaa aattcttcct tcagacaaca
      781 tcgctgacag atgccaaata attcaaaatg aaataactaa tggattgaag cagttttttg
      841 gtattgagcg tcaagtaaaa cttgaagttg cagtaaaaaa ttaccaacca aaacctcaaa
      901 acaaaaagac tgttagtcgt gtgaagtaag gaagtaaaaa atggaatggc ttaaacaata
      961 tcgatatcca attatcgctg gtctcatagg cgtatttctg gcttgtttga ttgtctcctt
     1021 tggcttcttc aaaacaatat ttgtattgat tttaggagca ctgggagttg cagctggatt
     1081 atatatcgaa aaaaactata tagataaata aaaaaataaa aattactaat ttaattaaag
     1141 gagtttcata tgtcaaacga aaaaaacaca aacactaacg tagaaaagaa agatgctact
     1201 gttgtagctc acgaaatcaa aggggaactt acttacgaag ataaagttat ccaaaaaatc
     1261 attggtcttt cactagaaaa cgtttcaggt cttttgggaa tcgatggtgg tttcttctca
     1321 aatcttaaag aaaaaatcgt taacagcgat gacgtaacaa gtggtgttaa cgtagaagtt
     1381 ggtaaaacac aagttgcagt tgacttaaac gttattgttg agtaccaaaa aaatgttcca
     1441 gctttatatt cagaaatcag agaaatcgta tcttcagaag ttgctaaaat gactgacttg
     1501 gaaattgttg aaatcaacgt aaacgttgtc gacatcaaaa ctaaagaaca gcatgaagca
     1561 gactcagtaa gccttcaaga tcgcgtatct gacgttgctg aatcaacagg agaattcact
     1621 tcagaacaat tcgaaaaagc taaatctggt cttggatctg gtttctcaac tgttcaagaa
     1681 aaagttagcg aaggtgtaga agctgttaaa ggtgcagcaa atggtgtagt atctcacgaa
     1741 aacactcgtg taaactaaga taaaataaat ataacaggag aaattatcat gtcagtagaa
     1801 gaaaaattaa atcaagctaa aggttctatt aaagaaggtg ttgggaaagc catcggtgat
     1861 gaaaaaatgg aaaaagaagg agcggctgaa aaagttgttt ctaaagtaaa agaagtcgcc
     1921 gaagatgcca aagacgctgt agaaggtgct gtagaaggtg ttaaaaacat gttgagtggc
     1981 gacgataaat aaggttaaaa gttactttat ctttttggta acattagtca agatgattct
     2041 cagaaaacaa aaagctagag attcccaatt gcggaactct agctttttaa ttttgcctct
     2101 ttctcttatt atatttcagc aggttgttgg ccatgagtat gaatcccatg tcaattctca
     2161 cttgacgctt ccctctcaga tgacatctct tataacccaa acaaaccttt atctgcccaa
     2221 agacagattt catatcaatc ttacgtttag cgaaaatttg tctacccttg gaagataaaa
     2281 gtgcctgata ttctttagtt tttaaacact ggtaacgttc attcatatac agtctctttt
     2341 gaggggctga ttcaggttca taatcgcagt caacattgat ttcaaggctg tttgctttct
     2401 atctcccctt tttcatgata tattttcgta caggtgcttc aatagtttct atagtaaaat
     2461 gaaccaaaaa tagtacacgt tgcggtataa tgttcttgtg gcatattcat tagattttcg
     2521 taaaaagttc tctcctattt gtgagcgaac aggtatagtc aatcgattgt cagtttgaag
     2581 accaagagtc gtaaggcaaa ggatatggca gaatccatcc aagggtggct ggcacaattt
     2641 ctagtgaacc tctttaaatc tattacgttt gactgtggaa aagagttttc aaaatggaaa
     2701 gatatttcca atcatcacga tagtgagagc ttttttgcga atctaggatg tccaagacag
     2761 cggtgtctca acgaacattc aaatagactt ttaaggtgtc atgacttgcc aaagcagaca
     2821 gatttcaacg aagtcagtca agaattttag gctgggtaaa aactcaattt caggagaaaa
     2881 tgaaataaat attcccacaa taaaacgcat agtatcaaag tttttccaac acctgatatt
     2941 atacattttt taattttaaa gactttttcc cagcctcaag aaatcacatc ccacctaaat
     3001 cctttggata ccaaatacct catcaggtat tttctgagtt ggtaagactg tctagcttaa
     3061 tttgacaaat ggagggcgtt aaatccagtt tttacaggaa gtctatttaa atattttcac
     3121 actgacctta caaaatatga aataaattat ataaagtcat tcccatgaca aagaataaca
     3181 tccctttaaa acagtatctt acccaatttt caggtaagac ttttgataca accgtcccca
     3241 atacccctcc gcatatagaa gcaagaaaaa taactggaat aggtgctaaa tgatactgat
     3301 ttaaacctgt aaccacaaca attgtagcca aatgagacaa ctgggaaaag aaaataattg
     3361 caagagaata catcgttgcg tcttttatcg aaatagaaaa aaacaccatc aataaagata
     3421 cattgagcgg accaccacca atccctaaaa aactagaaat caagcccaat agaagaccac
     3481 aaatcatata gaatcctatc ttatcaaatt gataagtttt tttaaaatct gttaaaacaa
     3541 atgatacaag taacatcaca aatagtaaaa tcatttgaac aatagatact agccgtactg
     3601 ataattgaga taacaaaact tgaaaaataa gatgacctag aaaccctcct aaaacagaag
     3661 aaaaggatac cgtaaaaatc aatctccatt ttatctcttt actttgtgaa aatcttttga
     3721 ttgttgaaat aatcgccatt ataaaaacag agaaactaga ataaaaagca atatcactga
     3781 ccgaatggta tccaaaacta tccataagtg gcttaattat aattccacca cccatgcctg
     3841 atatagaacc caatccacta gctattataa tgattagaaa ataaataatc tcaaccactt
     3901 tttaagtcca tcctattctt tcaaacatct aaaacctaga tggctactag tggaagtaga
     3961 aatacatcca tttctagcag ctactcggta tcgattacaa taagaacaat gacataaaaa
     4021 ggaacctcct ctgatagcat actcctcatc ttgtatccca tattttggta actcatagtc
     4081 atcttcatta aaactagcta ataaggtata tctcggattg cgacaccatt cccaaacatt
     4141 cccaatcatt tgatatagac caaaatcatt aggtaaaaat tcatagacag gagccgttcc
     4201 tataaatcca tctaaggcag tattttcata aggaaatctt ccttgccaag tattggcatg
     4261 ataatatcca ccttctaaaa gagtatctcc ccaaggatat tcagattgtc ttcctcctct
     4321 agcagcatat tcccactgtg cttcagtcgg taaagacatt ccgctccaat tacagaaagc
     4381 caatgcatct tctaaagcaa catgaactac tggatggtct tctaaaccca ctagattact
     4441 gttctcaccg tagggatgtt tccagcaagc gttcgaaact tgtaaccacc acggagcccc
     4501 agcaggatga ggatatccct ccctttctgc ctccggaacg aacaatataa aaacaaaaga
     4561 ccactcttgt cgctcagcta gtgttttata gcctgtttct ttcacaaatt gaacaaattc
     4621 ttgattcgta actggagtat ccgcaatgga aaaatccttt acactaacag cgacttgagg
     4681 accttcaaaa tcatctaaaa agccctcttc agaatttgtt cccatcataa atgtcccacc
     4741 cttgattttt ttcattttca tgattgacta tcccccgttt tcgttagagt tggcgttatt
     4801 ttagaaagtt caacaggaac taactcttca tccacaacaa aaccttcttc tctatatcta
     4861 agaaagtcta ctaatttttt cttcatctga cgaacaatag gttggtattt ttcacttgga
     4921 tacaaatcat tcatttcatg cggatctttt ttcatatcaa ataactgata atgatttaaa
     4981 acaggaaacc agataaattt ccactggtct gtcaagatat attgactaga atcttttcct
     5041 aaagcatgtt caccatgaaa ttctgttcgc cacccctcgt actgtccaaa taataaattc
     5101 ttaacactcc gtccatctag ttcatctgta gtagttcctc ctgccaaatc cacgagtgaa
     5161 ggaaaaatat cttgaatttt cactaattgc ttgattgttc ccctattacc agctatcaat
     5221 ccagccggat cgtaaataaa cgaaggaatg tggatacttc cctgataagg atatcccttt
     5281 ctaaacagat aatgttcacc aagctgatct ccatgatccg aaacaaacca gataatcgta
     5341 tctttatcat gacgaaattc ttttagggca gtcaaaaagc gactaatttg atggtctata
     5401 tgagtaatca atccaaaata agcagcaacc attcttcttt gatcatcttc ctttaatttc
     5461 cctcttaaag cataaataga cggaatttgc ttttctaata cttcccaatt cccgatatgc
     5521 aaatcgagaa attgaggcaa gcgttccata tacatatcaa aatagtattt tgggggattt
     5581 aatggagcat gtggtttttc aaaagacatt ttgagaaaaa aaggaaccgt tggatctctt
     5641 ctttgtaaaa atgaaatgga ctcactcaca acccagtttg ttggatgtaa cttctcatct
     5701 ttatcccaag gtctagcttc ccaagaatta caatccattc cgtcatcaat caaatcaaca
     5761 tcataaccaa ctttaccctt taaaaaggct agatagtcac tagcataatc aaattgactt
     5821 ccataagcct tatcatactt tctatcaaca tgtagatacc catcgtgaag taagacatga
     5881 tcaaacccaa gtctctgtct ggaaggaaaa acatgcatct taccaataca ctctgtttga
     5941 taacccatat ctttaaagac ttttggaaga gtattagtaa aattccaagg cacctcatct
     6001 tgataaccaa ctcgtccact tttatcttgg tctaagccag tcaataaagc tgccctagca
     6061 ggaacgcaag aaggaactgg agaataggca ttttcaaaat tataacctac actagccatc
     6121 atatctaatg ttggagtgct aactagctta tccttactat tcaaggataa ggcgtctgct
     6181 ctcatttgat ctacaacaat caaaataata tttggctgtt ttgtctgaac cataaaatct
     6241 cctttctaat atggcaaaag aggcacaaga agatatctac ctttactgca cccctttcta
     6301 tatcaatctc tctatataaa acaataacat tcttgttatg ttttatagaa aaatgaacta
     6361 aaatatgact aaatcgatta ggaaattcaa atcattttct agtactgttt tagaagttac
     6421 agtgtactat tccaacttca ataaattata aacctttgtc taataacaat tttagtggag
     6481 ataagaaatc ctacacctaa ctcatcttac acgtaatcta tttctatttt atcacaaaaa
     6541 acgcaagtaa gaccattaac tcaattcagt tttatatgcc attttcacaa atgggaaata
     6601 agtcaagaca ctaataatca aacaaacaac tgataagatg atggcacgcc aatcaaatgc
     6661 tgtagagaag aaaccatata aaattggagg cattacccaa gtaacgtttt gtgtaacagg
     6721 tgaaacaaga ccccagcttg ttgcccagta agctaccgtt gccatgaaaa ccgggctaag
     6781 tacaaatggt ataaatagca aaggattcaa gacaactggt aaaccataat tgaccggctc
     6841 accaatatta aacagaactg gtgctagacc aagtttagca acttttcgat aatgactgtt
     6901 tcttgaaaaa attaaaatag caagtactaa tcctaatcct ccaaaccaga caaacgcccc
     6961 aaaagaccca cttgtccata tataaggaat cggttcacct ttttggaaag catccagatt
     7021 cgctaacata gcaactccaa acagcccttc catgatggga gccaatacat ttcctccatg
     7081 gagaccaaaa aaccagaata acttattcaa aaagatcatc agaataactg caaagaaact
     7141 ttgagacaaa cctagtaatg gcgtttgtaa caccttgtaa acccaatcaa tcaataagtc
     7201 attgctaagt aaatggaaaa cataagtcaa gatggctact atatacatcg ccataaatcc
     7261 tggaatgata gaagtgaacg gcttagcaat cgcaggggga actgaatctg gtaacttgat
     7321 tacccagttc tttttcatta ctttacagaa aataatagag gctaaaaatc caatcatcat
     7381 ggctgtaaag tagcctctgg cattaatatg gtttcctgga atcacattcc caatagttac
     7441 catcagattt ttaccatcaa atgctagatt atcaattcct tgtaagattt gatctaattt
     7501 cacatctcct acatttgcca aagggaaact ctttgtaact gtacttccaa tcgaaatgac
     7561 aaacgaagca agcgatacca aaccagcaga aactgtatca acattgtaaa tcttagcgat
     7621 attcactccc aagcaataga tgaacaacaa ggaaacaatt ggtatacttc ccttgaatac
     7681 caaattattg atgtcaacaa gccactgaaa ggttttcgta atactttcta ggtgaaattg
     7741 ttgtggtaaa tccactagaa aagcatttaa taacaaagca atggaacctg tcataataac
     7801 aggcatagtc cccacaaatg aatcacgtaa agcaactaaa aacttctgat tcccaacttt
     7861 cattgcaatt ggagaaagtt tttcttgcat tttatccaac attgtattca taccaaactc
     7921 ctaatttttt tcaaatcgtt cgtatacttc taccacttct ttcgccaagt caacaaaggt
     7981 cagactcgtc attaaatgat cttgcgcgtg taccatcaag aggctaattg aagtttcttc
     8041 cccctgtgcc tcttttgtca acatttctgt ttgagccata tgcgccttta ataaggcagc
     8101 attcgcatca gctaattttt tattggcttt agagaaatcc cctttttttg ctgcctgaat
     8161 agcctccata gcattacttt tggcttcccc accatacata attagcccca ttacagattc
     8221 taaattactt tcattcatag cattctcctc aatcttagtc taataaactt tcagctaaat
     8281 ctaagacttt agaaccattc atcattccgt aatctgtcat cggaataacc gccacaggaa
     8341 tctgtctatc ttttagtttt tcttgaaagt cccctagtaa atagcgaact tgagggccta
     8401 aaagcaacac atttacttct tttgttgcta caatttcttc tgcttcagga gctggaactg
     8461 caaaaatttc tgcatccaac cccttatctt ctgctgcctt ttgcatcttt gtcactagca
     8521 gacttgtact catacctgct gcacatgcta acataattgt tactttagcc atagcttact
     8581 cctttatcac ctatttactg acctagccaa gcgagatact gtatgtctca ctcctctaat
     8641 tgccttgctc aatgcataaa tattatcgac tgttgccagt cccccatatc ctgcgtcacc
     8701 tatatgttga atatcaactc cacaaatttt atttctaagt gcaatttcct tgatagtatc
     8761 tgtatcagat gtttcttggc tagtaccaat agcactcaat acaagaccac ccttactatg
     8821 aacgagatca acgacttcac gcaactcttg gtcatgaaag gctggaacgg ttccaactgc
     8881 tggaacaagt atcacatccg cacctgcttc caacaattgc tctgcaacag aaagctctgc
     8941 cacaggctca ttcactcctg ccccgtgcat cttacctgca ataatcaaac cagaaaagtt
     9001 ttccttagca gtttgaacag ccttaatgat ttctcgattg ctaactcctg ttccaggatt
     9061 tccagtcaaa cagacaaagt caaaacctaa ctcctctatt cgcttcaatg tttcaacact
     9121 agcaacacga cctgcaacaa tttcctgtgt ttcctctagc atctttgcag atgggtcaat
     9181 cggttccaaa tttacaccaa ttggacaagc aacaagctgg tgtaagcgat gaataacatc
     9241 atcacctgaa ctatccaaag cataaatttt aggctcaaaa acatcaacac aattcaataa
     9301 aatcatatca gctccaaaag ctctagcaat ttcagaatta gttatatccc caacaaatgt
     9361 ctcacgagta actacatttt cagatagaac aactctacct tcacttgcta aaatactttg
     9421 ttttaattct tcagcattca tctgtaatat ctcagatgga tttgcactaa ttaaacgttt
     9481 cattctcaat cctccaataa cttaaatcgt ttttcaatag ctattacaaa tggtaaataa
     9541 agtaagatat ccaaaacaat caatacaatt tgtaataatg aaccagaaat acttcctgta
     9601 gcaagtaaac cacttataac aggtggcata gtccaacctg gatctgtata agttagcgga
     9661 accaaacctg atgccattgc tccccacgct actagtaagt taaacattgg agctaagata
     9721 aatggaacta gtaacaaaat gttcaaaaca ataggaacgc caaacattgc tggttcattt
     9781 atattaaata atccaggtat aacagttata ggtgccaaag tttttagttg tttactagct
     9841 ttcttcttat gtgctaaata tcctaacgca agaactaaac ctatggttgc accaccgcca
     9901 cctatgaata caaaattatc cataaatacg ctagtaaaaa tatttggaag agtttctcca
     9961 gtttgataag ctactttatt tgcatctaga ttagctaacc ataacggttt cataactgca
    10021 ttaacaacat ttcctccgtg taatcctaca aaccaaaaac tactattaag gaaaattaag
    10081 ataagaagac caataacatt atttcctaaa agtccaagtg gacctcccaa agctacttga
    10141 gcaatttcat gaagattagg taaacttaat ttatctaaag ttgcataaac aatcaaccaa
    10201 ccaacaatag tcactgcacc aggaataatg gcactaaagc ttttagatac tgctggtgga
    10261 acaccatccg gcatttttat ctgtatatta tgatttataa accattggta gatatagcca
    10321 ttaatcaatc ctaaaatcat agcaacaaat aaaccttttg atgccatgaa agcggtaggc
    10381 attcctgctc cagcctctcc tctaatgaaa ggcgtaactg taataaaaga tgataatgac
    10441 acaataccag caggaatacc atcaactttg tattgtctag ccataaagta agcgatacca
    10501 aaactagcta caagaccaag taatccaaaa ctactatcaa cacccttcca taaatagtca
    10561 gcaacaccaa tatcacccag atatttttcc catcctggaa taggaaaact tgcaataacc
    10621 attagtaatg aacctatcaa caataaaggt atagttagag taataccgtc tcgaatagaa
    10681 accaacgctt tattattacc caatctagcc gctactggaa gtaacttttc ttgtaataat
    10741 ttttccata
//
LOCUS       DBERMR010000068        10605 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.068, whole genome shotgun sequence.
ACCESSION   DBERMR010000068 DBERMR010000000
VERSION     DBERMR010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10605
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.068"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            1..607
                     /locus_tag="ACL8A1_001925"
                     /pseudo
     CDS             1..607
                     /locus_tag="ACL8A1_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            507..953
                     /locus_tag="ACL8A1_001926"
     CDS             507..953
                     /locus_tag="ACL8A1_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGS1202634.1"
                     /translation="MRKHGLISKSTSKRYYQVAIPFTRLFCPTLVSIDYINCLSETID
                     KNWHKSDRIFVTNTGKPVHSSILSKSLQRANERLKKPIPKHLSPHIFRHTTISILSEN
                     KIPLKTITDRVGHSDSEVTTSIYTHVTKNMKDEAINVLDKVMKKIF"
     gene            complement(1091..2395)
                     /gene="eno"
                     /locus_tag="ACL8A1_001927"
     CDS             complement(1091..2395)
                     /gene="eno"
                     /locus_tag="ACL8A1_001927"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-displayed alpha-enolase"
                     /protein_id="HGS1202635.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            2559..3005
                     /locus_tag="ACL8A1_001928"
     CDS             2559..3005
                     /locus_tag="ACL8A1_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HGS1202636.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(3002..4117)
                     /locus_tag="ACL8A1_001929"
     CDS             complement(3002..4117)
                     /locus_tag="ACL8A1_001929"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HGS1202637.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVEYLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     ASGQSLLNLASVSTENCYKIPEGVQIHILADVVSPLCGHQGATYTFGNQKGLHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGSGGGLAGGLCAFAQASIVSGIDTCLDLINFNKKV
                     SDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQAV
                     FSILEKSEPLEDSLKNASLYLERTAANIGHLLNMYKI"
     gene            complement(4126..4248)
                     /locus_tag="ACL8A1_001930"
     CDS             complement(4126..4248)
                     /locus_tag="ACL8A1_001930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HGS1202638.1"
                     /translation="MKSAELGIAFCAKEVLKKEIPHHVDKRDFLEVLPLIDRLE"
     gene            complement(4334..4705)
                     /locus_tag="ACL8A1_001931"
     CDS             complement(4334..4705)
                     /locus_tag="ACL8A1_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HGS1202639.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHCQLA"
     gene            complement(4764..6197)
                     /gene="glgA"
                     /locus_tag="ACL8A1_001932"
     CDS             complement(4764..6197)
                     /gene="glgA"
                     /locus_tag="ACL8A1_001932"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HGS1202640.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGLERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(6194..7333)
                     /gene="glgD"
                     /locus_tag="ACL8A1_001933"
     CDS             complement(6194..7333)
                     /gene="glgD"
                     /locus_tag="ACL8A1_001933"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HGS1202641.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAEMFNMSTDIFVVDTPWLIEHLEEEAKKEHPKK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GKGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(7323..8465)
                     /locus_tag="ACL8A1_001934"
     CDS             complement(7323..8465)
                     /locus_tag="ACL8A1_001934"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HGS1202642.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGAVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(8455..10383)
                     /gene="glgB"
                     /locus_tag="ACL8A1_001935"
     CDS             complement(8455..10383)
                     /gene="glgB"
                     /locus_tag="ACL8A1_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HGS1202643.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPV
                     AVRYEARPGTGAIVTELPDKKWRDGLWLARRKRWGFAERPVNIYEVHAGSWKRNPDGS
                     PYSFAQLKDELIPCLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDNAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         3235 a         2334 c         1879 g         3157 t
ORIGIN      
        1 gcttatttga ctgaaatagc ttctgaattt ggctgtcatc caactaccat ccactatgcg
       61 ctcaaagcta tgggctacac tcgaaaaaaa gaaccacacc tactatgaac aagacccaga
      121 aaaagtagcc atatttctta agaattttaa tagattaaag cacctagcac ctgtttagat
      181 tgacgaaaca ggattcgata cttattttta tcgagaatat ggtcgctctt taaaaggtca
      241 gttaagaaga agatatcaga ggatttcttt ggttgcaggt ctaaccaatg gtgagttaat
      301 cgctccaatg acttacgaag agacgatgac gagcgacttt tttgaagcat ggtttcagaa
      361 gtttctctta ccaacattaa ccacaccatc ggttattatt atggataatg caagattcca
      421 tagaatgggt aagttagaac tcttgtgtga agagtttggg cataaacttt tacctcttcc
      481 tccctactca cctgagtaca atcctattga gaaaacatgg gctcatatca aaaagcacct
      541 caaaaaggta ttaccaagtt gcaatacctt ttacgaggct cttttgtcct actcttgttt
      601 caattgacta tataaattgt ctaagcgaaa caatcgataa gaattggcac aaaagcgacc
      661 gtatttttgt taccaataca ggaaaaccag ttcatagttc tatcttgagc aagtctctcc
      721 agcgagcaaa cgaacgcctt aaaaaaccaa ttcccaaaca tctgtcccct cacatcttca
      781 gacacaccac tattagcatc ttatcagaaa ataaaattcc tttaaaaaca atcacggaca
      841 gggttggtca ttccgactct gaagtcacta cttccatcta cacccacgtc acaaagaaca
      901 tgaaagatga agcaatcaat gtactggata aagttatgaa aaagattttt taaaaagttt
      961 tgtccctttt ttgtcctcta aatacaaaaa tagcccttcg gataaaatcc gaggagctag
     1021 aaacgttgtt aaatcaacgg ccgaactttt gaatttcatg gttcgggata aaatagttca
     1081 ctgaactatt ttatttttta aggttgtaga atgatttcaa tccacgatat tcagctactt
     1141 caccaagttg gtcttcgata cgaagcaatt ggttgtattt agcgatgcgg tctgtacgtg
     1201 aaagtgaacc agtcttgatt tgtcctgcgt tagttgcaac tgcaatatca gcgattgttg
     1261 aatcttcagt ttcacctgaa cggtgtgata caacagcagt gtaaccagct tctttagcca
     1321 tttcgatagc ttcaaaagtt tcagtaagag taccgatttg gttaactttg ataaggattg
     1381 agttagcagc accttcttgg ataccacgtg caaggtagtc agtgtttgtt acgaagaagt
     1441 cgtcaccaac aagttgtact ttcttaccaa gacgttcagt aagagctttc caaccatccc
     1501 agtcgttttc atccatacca tcttcaatag tgatgattgg gtatttgtta accaattctt
     1561 caaggtagtc gatttgttct gcagatgtac gaacagcagc accttcacct tcaaatttag
     1621 tgtagtcgta aactttacgt tctttatcgt agaattctga tgaagcacag tcaaatccga
     1681 taaatacgtc tttacctggt acatatccag cagcttcaat cgcagcaagg atagtttcaa
     1741 caccatcttc agttccttcg aaacgaggag cgaatccacc ttcgtcacct acggcagttt
     1801 ccaaaccacg tgatttaagg attttcttaa gagcgtggaa gatttcagca ccgtaacgaa
     1861 gggcttcttt aaatgttggc gcaccaactg gcaagatcat gaactcttgg aaagcgattg
     1921 gagcgtcaga gtgagaacca ccgttgatga tgttcatcat tggagttgga agaactttag
     1981 tgttgaatcc accaagatag ctgtaaagtg ggatttcaag gtagtcagca gcagcacgag
     2041 ctacagcgat agacacaccg aggattgcat tcgcacccaa tttacctttg ttaggagtac
     2101 cgtcaagtgc gatcatagca cggtcaatag cttgttgatc acgtacatcg tagccaatga
     2161 tagcttcagc aatgatgttg tttacgttgt caacagcttt ttgtgtacca agaccaccgt
     2221 aacgagattt gtcaccgtcg cgaagttcaa ctgcttcgtg ttcaccagta gaagctcctg
     2281 atggaaccat accacgtccg aaagcacctg attcagtgta aacttctact tcaagtgttg
     2341 ggttaccgcg tgagtctagg acttcgcgag cgtaaacatc agtaataatt gacatttttt
     2401 actctcctta tgagttaaat tttttacacc tctataatac cttaaaaccc ctcctttttc
     2461 aagaaaaaac gttatctttg tgcaactttt ccttaacttt ataaagtaat cgctttcttt
     2521 tgtctgtttt attctaactt ttatgatata ctgttttcat gacagattta tcaaaacaat
     2581 tacttgaaaa agctcatggt gggttaaaaa taaatccgga tgagcaaaga cgctatcttg
     2641 gtacttttga ggaaagagtt cttggatatg tagatattga cacagcaaat agccctcagt
     2701 tagaaaaagg ctttttattt attttagaaa accttcagga aaaagcagag ccactatttg
     2761 tgaagatttc accaactatc gaatttgata agcaagtttt ctacttaaaa gaagcaaaag
     2821 aaactgatag tcaagccacc atagtatctg aagagcatat tacttctcct tttggcctgg
     2881 ttattcatag caatgcacca gttcaagtag aagaaaaaga ccttcgactt gcttttccaa
     2941 aactttggga agttaaaaag gaagaaccag ccaaaacatc cttatggaag aaatggttta
     3001 gctaaatctt gtacatattt aataagtgcc caatattggc agccgtgcgc tccagataga
     3061 gactggcatt ttttaaacta tcttctaaag gttcactttt ctccaaaata gaaaagacag
     3121 cttggatatt ttcaaatggt aggggaggta aatcttcagc aagactaccg caaatagcaa
     3181 taacaggaac tccaacaggg gttctttttg caacacctat aggcgctttc ccagcaaagc
     3241 tttgactatc aagccttcct tctccaacaa caaccaagtc agcatctgaa actttcttat
     3301 taaagttgat taagtccaag caggtatcaa ttccagacac gatacttgcc tgagcaaagg
     3361 cacacaaacc accagcaagg cctccacctg atcctgctcc tttaatttct aatgttgcag
     3421 gtgagaattt ttcataaaaa tcttggatcg cctgatctac gactgcaaac atagtcggat
     3481 gtagaccttt ttgattacca aaagtgtaag tcgcaccttg atgaccacat aagggactca
     3541 cgacatctgc tagaatatga atttgaacac cttcaggaat tttatagcaa ttttctgttg
     3601 aaacagaagc taagtttaat aaggattgac cggaagcagg caagacattt ccatccctat
     3661 cataaaattg ataacctaaa ccagcagcaa tccccagtcc tccatcatta ctggccgtgc
     3721 caccaacacc gatataaata tctttaatcc ctttagaaat gagatggaga atcaactctc
     3781 caataccaca agtttggatt tgaagtggat ttcgcttctc tagcggaatt tttccaagac
     3841 caaccaaatc agctacttca aatagtgcca gttccccttt ttgaaaatag cgcatggctt
     3901 ctttttgtcc aaaagggtct gtcacttgga tccatttttc ttttaggtca agagaatgtc
     3961 ggatagcatc tacagtacct tctcccccat caccaacagg gcagaggaga tattctacat
     4021 ctgctatcga ttgttggaag cctcttttta ttgcttcagc tacctgttga gctgtcaagc
     4081 tttccttaaa cgaatccggt gcaattacaa tcttcatatt ttccctcatt ctaaacggtc
     4141 aatcaaagga agaacttcta aaaaatccct cttgtcaaca tgatgtggta tttctttttt
     4201 gagcacttct ttggcacaaa aggcgattcc taactccgcc gacttcaacc tccatttttc
     4261 cagagtctct tttttgacct ggggacttat aatttgtcca actaattttc ctgttaaaag
     4321 accttctttg acttcaagct agttggcagt gaaataggca ataccaaggg attttgctaa
     4381 tctctccaac tattggtgta aatccaccag acaccagacc aactaggatg ccattctttt
     4441 ggagaataga gatgaactct gggacattta gcgatagatg aattgagttg aagacgttat
     4501 caaagaccaa aataggaaga ccttccaaca aggacactct ttttcttaaa ctgctttcaa
     4561 agaccaactc tcctcgcatt gctcgacttg taatctgcga aatttccgcc tcatgacctg
     4621 cctctctccc taaaagatca atcacttctt ctaggattaa ggttccatct acatccaaaa
     4681 cacacaagcc ttttacttga gacatcagtt ctcctctcta aacagcctaa aaatcgtatg
     4741 aagtcatcat acgattttat ctattaatta actaaactat ggtacaagtc aaggtatgac
     4801 ttgcaggctg tatcccatga gaagtcactc tccatagctt gtttttgtag gtttctccaa
     4861 atgtctggat ggtttctata caagtccaat gctgtttgga aagtccaatt taaccaataa
     4921 ggagatagat tgtcaaagct aaagccagta ccgcttcctt cgattggatt gaaagcgcga
     4981 actgtatctc gcaagcctcc aacttcatgg accaatggca aggttccata acgcatagcc
     5041 atcatttgag acaagccaca cggttcaaaa cgacttggca tgaggaagag gtcacaagca
     5101 gcgtagattt cctgagcaag tttgacatca aaagtgatat ttgttgatag cttgtctggg
     5161 taaatctgag caaaccatga gaaagctcct tcaaaggctg gatcgccagt tcccaaaaga
     5221 acaatctgaa catcttcttg caagatatgg tgaagacttt cgaccaccac atcaaaacct
     5281 ttttgacgtg tcaaacgaga aacaattccc accagtggaa cgtctgctct aacaggcaag
     5341 ccaactcttt cttgcaattt tgccttattt ttggctttcc cagacaaatc ttcctgattg
     5401 aaatgatagt ctaaaagagc atccgtctga ggattataaa gatcagcatc aatcccattc
     5461 acgataccag atactttacc agactccatt ttaagaatct gatccaaatt acatccaaac
     5521 tgactagtca taatttcatg agcatagcta ggtgaaacgg ttgaaacacg gttcgcatag
     5581 agaatacctg ccttcatcca gttcagacag ttgttccatc gaagggtgcc atcagcgtaa
     5641 cgttcaaggc caactccaaa caaatcaccc aacattcctt ctgaaaattg tccttggaat
     5701 tctaaattat gaatggttaa aactgtttca atgtcctcat aggcttgaat ccaacggtat
     5761 ttttccttca acaagaaagg aatcatagct gtatggtagt catgaacatg gagaagatca
     5821 ggaataaagt caatcctttc catagcctca atggcagcca gttggaaaaa ggcaaagcgt
     5881 tctccgtcat caaaatcacc gtaaacatga ccacggaaga aataatattg attgtcaata
     5941 aagtagaagg ttacaccatt taatactgtt ttcttaattc cacaatactg tctgcgccaa
     6001 ccaacgctca cctcaaaatg aagcacatct tcaatctgat ttccaaattt agcctctacc
     6061 atatcatagt agggtaaaat cactgcaact tcgtgcccag cttttaccag tgattttgga
     6121 agagcgccaa tgacgtctcc caaaccacct gtttttgaaa aaggtgcacc ctctgctgct
     6181 acaaataaaa ttttcatgaa tgaatatcct ctgttacttt agcacctttc ttaaccacaa
     6241 ctggatgttc tgcagttcct cgaatcacaa caccaggctc aacttcaacc cctttgtcca
     6301 agatagcata ttcgacctga gccccttttc caataacaac acgagggaag agcaggctat
     6361 ctttaaccaa gctatcctta tggacatgaa tattacgtga tagaacagaa ttagctactt
     6421 gaccttcaat aatactacca gaggcaaact gagaagtgct taccttagat gtattagcat
     6481 agtaagttgg ctcttcgttt ttgacctttg tataaatctt ttggtttggt gagaaaagag
     6541 aatagaattt ttgtgattca agcatatcga tattcgcttg ataataagat ttaacagagt
     6601 gaatattggc tagatagccc gtgtactcgt aggcgaaagc tccctctttt acagccaaat
     6661 cccgtaaaac atagcgcaat ttctttggat gttctttttt agcttcttct tccaagtgtt
     6721 caatcaacca aggtgtatca acgacaaaga tatctgtaga catattgaac atttcagctg
     6781 ttgacttgct atcaaagagt ttatgagaaa gaacatggtc tgtttcatct acatccaaga
     6841 ttgcatttac ttctgaaata tctttcttag ctagtttttt ataaactaca gtgataggct
     6901 cttttgttgt actatgtagg tggaaaactt ggttcaaatc aatgttaata agaacatcgc
     6961 agttgagggc aaccgtttgg tttgagccag aacgtttcaa ataagtaaga agctgttggt
     7021 agtattcttt tccaactgta ctactttcta cacgggtatt gtaaattcct agatagtaat
     7081 gactaagaag ggttgataag ccccactcgc gtcctgaacg aatatggtca aatactgagc
     7141 tgatattatc ctgctggaaa ataccaaaga cactacgaac acctgcatta gcaaggcttg
     7201 aaagtgggaa gtcaatcaaa cgatatttcc caccaaatgg caaacttgct actggacggt
     7261 ggtccgtcaa tgtcgacata ttgtgaaaac caactgtatt tcctaaaatg gcagaatatt
     7321 tatcaatctt catctgttgc tacccccact acttcattat atcctacaac ttgtacttca
     7381 tctgttccat caatttcgac accgtcagaa ataatcgcac cttcaccaat aatggcacgt
     7441 ttaatcttag ctccttgacc aatgatagct ccactcatga taactgaatc aaggacttcc
     7501 gctccttcgc gaacttgcgc gcctgttgaa aggatagaat gtttaacagc tccatcaacg
     7561 aaacatccgt ctacaactaa tgagtcttcc acatgagcat ttgccccgag gaagtttggt
     7621 ggtgaaatca agtttcttga gtaaatcttc cattgacggt tacgactatc caaggcattt
     7681 tctggagaaa tatactccat gttcgcttcc caaagtgact caatagtacc aacatctttc
     7741 caataaccac taaattcgta agcataaaca ctttcacctg actcaaggta atttggaatg
     7801 acatttttac caaagtctga catgccaacc ttgctctttt cagcagcgac taacatatta
     7861 cgaaggcgtt gccaatcaaa aatgtagatt cccatagaag cttttgtaga tttaggttga
     7921 gctggttttt cttcaaattc aacaatacga ttgttagcat ctgtgttcat gataccaaaa
     7981 cggcttgctt ctttaagagg gacgtctaaa actgctactg tcaagctggc attattatcc
     8041 ttatgagact ggagcatatc atcatagtcc attttgtaga tgtggtcccc agacaaaatc
     8101 aagacatact caggattgac actgtcgata tagtcgatat tttggtaaat agcgtgacta
     8161 gttccctcaa accaacgatt tccttcactt gcagaataag gttgaagaat agagacgcct
     8221 gaattaatac cgtctagtcc ccagcttgaa ccattcccaa tatggttgtt gagagcaagt
     8281 ggttgatact gtgtaacgac cccaacatta tgaatccctg agttggcaca gtttgatagg
     8341 gcaaagtcaa tgatacggta gcgcccacca aattgcacag ctggttttgc gatgctttga
     8401 gtgagtttac cgagacgagt tccttgccca ccagcaagaa tcaaagctaa catttcattt
     8461 ttcattttct actccttttt ggtttttatt tgtgacggtt ttagtagatt tcaagcgacg
     8521 tttgattttc catacacttg ctcccatagc cggtagagta aaggttaagg tctgctcata
     8581 atctttccat aggccttctt gcgtttgaac agtttgatta tgttctttcc aaacgcctcc
     8641 ccattcttcc aactcagtat tccatacttc ttcgtaaatt cctgcaacgg gtagtccgat
     8701 tgtaaaatct ttccgctcaa caggtaccat gttaaagata cagactaaca tttctccctt
     8761 tttaccctta cgaataaagg aaagaacact ctggtctcga ttatccgcat caatgatttc
     8821 aataccatca tagctggtat cgatttccca cagacagcga tgatctttgt aaaactggtt
     8881 tagctgagaa gtgaaatact tcatcttagc attcattggg tcttctaggt tagaccattc
     8941 caactgttct tcagatttcc attctaggaa ttgaccgtat tcgctaccca tgaagagcaa
     9001 tttcttacca gggtgacaaa tttggtacgt atagagattg cgcaagcctg cgaattgatt
     9061 gtaacgatct ccccacatct tatgcatcat actcttcttg ccatgaacca cttcatcgtg
     9121 cgagaatggc aagagataat tctccttgaa aacatacata aagctgaaag tcaccaggtt
     9181 aaagtcatat ttacgataaa tcggatcttc ttcgtagaaa cggaggatat cattcatcca
     9241 gcccatgttc catttgtagt caaatcctag accaccaatc tctttcattc ccgtaatctt
     9301 ggtcgcagac gaactttctt ctgcaatcat catcacatct ggatattcta acttaataac
     9361 ttcattcaag cgttgaagaa aataataacc ttcatagttg agatttccgc catctttatt
     9421 aggtgtccat ggagcattat catagtccaa atagagcatg ttgctaacag catccacacg
     9481 aataccatcc aaatgataga catcaatcca atgcttaatg caagaaatta agaaggactg
     9541 gacttcattt tttccaaggt caaaattaag ggcaccccaa ccatggttat gagccttatt
     9601 atggtcttgg tattcaaaag tcggtgtccc atcataatag gctaaggcat catcgttgat
     9661 ggtaaagtga cctggtaccc agtccacaat aaccccaata ttatgggtat gacactcctc
     9721 gacaaaatct tgaaactcct ctggtctgcc ataagcatgc tctaaagcga agtaacccat
     9781 aagctgatac ccccaactca agcccaaagg atgggacatc aagggcataa actcaatatg
     9841 agtatagttc atttcaacga gacaaggaat gagttcatcc ttgagctggg caaaactata
     9901 aggactgcca tcaggatttc tcttccaaga gccagcatga acctcgtaaa tattgacagg
     9961 acgttcagca aagccccaac gttttcttcg tgccagccaa agtccatccc tccatttctt
    10021 atcaggaagc tctgttacga ttgcccctgt tcctggacga gcctcatacc tgacagcaac
    10081 agggtcaatc ttcatcagtt gatgaccatt ttgacgtgtg acatgatatt tgtaaatatg
    10141 cccttcttga gccatattgg taaagacttc ccagacccca aagtcatttc ttaccattgg
    10201 aatctgattt tcaatccagt tggtaaaatc accaaccaag tgaacagcct gagcattagg
    10261 tgcccaaaca cggaaggtat agccatgctc tccatttagt tcttccctat gtgctcctag
    10321 ataatgttgg agataaaaat tttcacccgt cataaaggtt tttaatgctt ctctattatc
    10381 catatactcc ccttctcctg taagcgtttt ctatgttttt attatactac ctttttagag
    10441 aagattcaag taaattacta tacttcttta attattttga aaatctacaa caagttcact
    10501 tactcgttca attgtaaatc aatatttttt caaaaaattg cgaaaacgcc tttctttttc
    10561 tactatagtg aaatgaaata aaacatgcgc aaatcgatca ggaca
//
LOCUS       DBERMR010000069        10325 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.069, whole genome shotgun sequence.
ACCESSION   DBERMR010000069 DBERMR010000000
VERSION     DBERMR010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10325
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.069"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            89..1264
                     /gene="mutY"
                     /locus_tag="ACL8A1_001936"
     CDS             89..1264
                     /gene="mutY"
                     /locus_tag="ACL8A1_001936"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HGS1202644.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            1319..2023
                     /gene="yycF"
                     /locus_tag="ACL8A1_001937"
     CDS             1319..2023
                     /gene="yycF"
                     /locus_tag="ACL8A1_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HGS1202645.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            2016..3365
                     /gene="vicK"
                     /locus_tag="ACL8A1_001938"
     CDS             2016..3365
                     /gene="vicK"
                     /locus_tag="ACL8A1_001938"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HGS1202646.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            3367..4176
                     /locus_tag="ACL8A1_001939"
     CDS             3367..4176
                     /locus_tag="ACL8A1_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGS1202647.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            4267..4509
                     /gene="relB"
                     /locus_tag="ACL8A1_001940"
     CDS             4267..4509
                     /gene="relB"
                     /locus_tag="ACL8A1_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HGS1202648.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            4511..4765
                     /locus_tag="ACL8A1_001941"
     CDS             4511..4765
                     /locus_tag="ACL8A1_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGS1202649.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            4812..5585
                     /locus_tag="ACL8A1_001942"
     CDS             4812..5585
                     /locus_tag="ACL8A1_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HGS1202650.1"
                     /translation="MKKKWFFADYYDTTIILLALISVILVLLGFAEMIDLDNPPYSII
                     DLVIWGVFVIDYSWRFFTTKRKWRFILENIFDLLAILPLNAIFTVFRLGRIFRLAKLT
                     KLLKLTRLLRIIGLTGKLERKISRFLRTNGLIYILYVNIFIVLVGSSILSVVEEKSFS
                     DSLWWALVTVTTVGYGDIVPASIFGKWLAVLLMLVGIGTIGMLTSALTNFFVKDNPDE
                     QIKLDKLQDELSSQRILLEKQSKKIEELHKMIQDLIEKT"
     gene            complement(5848..6834)
                     /locus_tag="ACL8A1_001943"
     CDS             complement(5848..6834)
                     /locus_tag="ACL8A1_001943"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HGS1202651.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            7028..9496
                     /gene="gyrA"
                     /locus_tag="ACL8A1_001944"
     CDS             7028..9496
                     /gene="gyrA"
                     /locus_tag="ACL8A1_001944"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HGS1202652.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMVGQILSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            9496..10239
                     /gene="srtA"
                     /locus_tag="ACL8A1_001945"
     CDS             9496..10239
                     /gene="srtA"
                     /locus_tag="ACL8A1_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HGS1202653.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTAFNQPYKQFY"
BASE COUNT         3209 a         1738 c         2309 g         3069 t
ORIGIN      
        1 agtagataga aaagttatat actatctatt gaaaatatta taaatcagat acaaagacag
       61 gtgaaacctt ctgtgttata ctagagatat gttagatttg aaagaatacg gtatcgtcat
      121 gtggccggag gagaaagtca tttctttccg tgagaaactt ctcgcttggt atgatgaaaa
      181 caaaagagat ttgccttgga ggagaagtaa aaatccttat cacatctggg tatctgaaat
      241 catgcttcag cagaccaggg tggatacagt tatcccttac tacgaaagat ttttggactg
      301 gtttccaact gtcgaaagtc tggcaactgc gcctgaggag agtttactga aagcttggga
      361 gggcttgggc tattattctc gagttcgcaa tatgcaggct gcagcccagc agattatgac
      421 tgactttggt ggccaatttc caaataccta tgaaggaatt tccagcttga aagggattgg
      481 accttacaca gcaggagcca tttccagtat tgcttttaac ttgcctgagc cagctgtaga
      541 tggtaatgtc atgcgggtct tggcgcgtct gtttgaagtc aaccacgata ttgggattcc
      601 aagtaatcga aaaatttttc aggcaatgat ggagatcttg attaatccgg atcgtccggg
      661 tgattttaat caagcattga tggacttagg gtctgatatt gagtctcctg taaatcccag
      721 acctgaagaa agcccagtca aggactttag tgcggcatat cagaatggaa ccatggaccg
      781 ttatccaatt aaatctccta agaaaaagcc ggttcctatt tatcttaaag ccttagttgt
      841 gaaaaattct caaggacaat ttttacttga aaaaaatgaa agcgaaaagc tcttagcagg
      901 tttttggcat ttccccttta tagaagttga taacttttcg caagaagagc agtttgacct
      961 ctttcatcag gttgcggaag aaagtgtgaa ctttggcccc agtccagaag agagtttcca
     1021 gcaggactat gacctagatg ttgattggct tgatgtttgt tttgatacag tccagcatgt
     1081 ttttagtcat cgaaaatggc atgtacagat tgtagcaggt caggtgagtg acttccatga
     1141 tttttcagat agggaagttc gttggctttc accagaagaa ttcaagaatt acccacttgc
     1201 caaaccccaa caaaaaatct ggcaggctta tgcacaagcc aacttagaca gtagccaaga
     1261 ctagctattg tgtcttctga ctattttttg gtataatagt agagaaaaag gtgaacatat
     1321 gaaaaaaata ctaattgtag atgatgagaa accaatctcg gatattatca agtttaatat
     1381 gaccaaggaa ggttacgaag ttgtaactgc ttttaatggt cgtgaagcgc tagagcaatt
     1441 tgaagcagag caaccagata ttattattct ggatttgatg cttccagaaa ttgatggttt
     1501 agaagttgct aagaccattc gtaagacaag cagtgtgccc attcttatgc tttcagccaa
     1561 agatagtgaa tttgataagg ttatcggttt ggaacttggg gcagatgact atgtaacgaa
     1621 acccttttcc aatcgtgagt tgcaggcgcg tgttaaagct cttctgcgtc gttctcaacc
     1681 tatgccagta gatggtcagg aagcagatag taaacctcaa cctatccaaa ttggggattt
     1741 agaaattgtt ccagacgcct acgtggctaa aaaatatggc gaagaactag acttaaccca
     1801 tcgtgaattt gagcttttgt atcatttagc atcgcataca ggtcaagtca tcacgcgcga
     1861 acacttgctt gagactgtct ggggttatga ctattttggt gatgtccgca cagttgatgt
     1921 gactgtacga cgtctgcgtg agaagattga agatacgccc agccgaccag agtatatctt
     1981 gacgcgccgt ggtgtagggt attacatgag aaataatgct tgatttactg aaacaaacca
     2041 tttttaccag agattttatc tttatcctga ttttgttagg tttcatcctt gttgtgaccc
     2101 tcttattact ggaaaataga cgtgataata ttcagttgaa gcaaatcaat caaaaggtta
     2161 aagatttgat tgcaggagat tattccaagg ttcttgatat gcaaggtggg tctgaaatca
     2221 ccaatattac caataatttg aatgacttgt cggaggttat tcgtctcact caggaaaatc
     2281 tagaacaaga gagtaagagg ctaaatagta ttctgtttta tatgacagat ggggttcttg
     2341 cgactaaccg tcggggtcag attatcatga ttaacgatac agccaagaag caactggggt
     2401 tggttaagga agatgttctg aatagaagca ttttggaatt gctcaagata gaagaaaact
     2461 atgaattgcg tgatttgatt acccaaagtc cagaattgtt gctagattcc caagatatca
     2521 atggtgaata tttgaacctt cgagttcgct ttgccttgat acgtcgagag tctggcttta
     2581 tttcaggttt ggtggctgtt ttgcatgata cgacggagca ggagaaggaa gaacgcgaac
     2641 gaagactctt tgtttccaat gttagccatg agttacggac tcctctgact agcgtaaaat
     2701 cctatcttga agccttggat gagggggctt tgtgtgaaac tgtagcacca gactttatca
     2761 aggtttctct tgatgagacc aaccgtatga tgcgcatggt gacggatctc ctccatcttt
     2821 cacgtattga taatgctacc agtcacctag atgtggaact gattaacttc actgctttta
     2881 ttacctttat cctcaatcgt tttgacaaga tgaaaggaca ggaaaaggag aaaaaatatg
     2941 agttggtgag agattatccc atcaattcta tctggatgga aattgataca gataagatga
     3001 cgcaggttgt cgacaatatt ttaaataatg ctattaagta ttcgccagat gggggtaaaa
     3061 tcactgtcag aatgaagaca actgaagacc agatgatttt atccatttct gaccacggtt
     3121 tggggattcc taagcaggat ttaccacgta tctttgaccg tttctatcgt gtggatcgtg
     3181 ctagaagtcg tgcacaaggt ggtacaggtc taggactgtc tatcgctaaa gaaattatta
     3241 aacaacataa gggctttatt tgggccaaga gtgaatacgg caagggttca acctttacca
     3301 ttgtgctccc ttatgataag gatgcagtga aagaagaagt atgggaggat gaagtagaag
     3361 actagaatga gtgaaatagg ctttaaatac agtattttag catcgggttc cagtggaaat
     3421 tctttttatc tggaaacctc aaaaaagaag cttttagtgg atgcaggctt gtctggcaag
     3481 aaaattacca gtctgctagc tgaaattaac cgcaagccag aagacctgga tgccatcttg
     3541 attacccatg agcattcaga tcatatccat ggagtaggcg ttttggctcg caagtatggt
     3601 atggatcttt atgccaatga aaagacctgg caagctatgg aaaatagtaa atatcttggc
     3661 aaggtggatt cttcgcaaaa gcatattttt gaaatgggta aaaccaaaac ctttggagat
     3721 atcgacatcg agagttttgg tgtaagccat gatgcagtcg caccgcagtt ctatcgcttt
     3781 atgaaggatg ataagagttt tgtccttttg acagatacag gttatgtcag tgaccgtatg
     3841 gcgggcattg tcgaaaatgc ggatggctat cttatcgagg ccaaccatga tgtagagatt
     3901 ttgcgatcag gttcttacgc ttggcgactc aaacaacgaa tcctatctga ccttgggcac
     3961 ctttctaacg aggacggtgc tgaagctatg attcggacgc taggaaatcg tactaagaag
     4021 atttaccttg ggcatttatc taaggaaaat aatatcaagg aactggctca tatgaccatg
     4081 gtcaatcagc tggctcaagc tgatctggga gtcggagtag actttaaggt ttatgatacc
     4141 tcaccagata ccgcaacacc attgacagag atatagaaag aacgctgaga aggtgttctt
     4201 tttatattga ctgaacacct aaaaagtaat acaatggtgt taccattaaa aaagggagca
     4261 caaaagatga ctactattac attaaaagtt tctgaagctg ataaaacatt tatgaaagca
     4321 atggctaagt ttgaaggagt ttccctttcg gaacttattc gcaccaaaac tcttgaagcc
     4381 ctagaagatg aatacgatgc tcgtgtggca gatttagcct atcaagagta tttagaagac
     4441 ttggaaaaag gagttgaacc cattacttgg gaagaaatga tgcatgattt aggcttgaag
     4501 gatgaataat ttgtataaat tagttccaac aagacgtttt atcaagcaat tgaaaaaatt
     4561 ggaccgttat acgcagaagc taattacaaa ctatttacaa accaatgttt tggaagaccc
     4621 aagacgacac ggaaaggctt tggttggtaa tcgcgttggt caatggcgct atagaattgg
     4681 taattatcga gttatcgtac aaattgtaga tgatgaatta gtcgttgcta ctctagaagt
     4741 tggtcatcgg agagatattt attgaattaa agctgagtaa taacaatata gaaagtaagg
     4801 agagtaaaaa tttgaagaaa aaatggtttt ttgcagatta ttatgataca acaattattt
     4861 tacttgcttt aatctctgtt attctggtat tacttggatt tgctgagatg attgatttgg
     4921 acaatccgcc ttatagtatt attgatttag taatctgggg tgtttttgta attgattata
     4981 gttggcgctt ttttacgact aaaagaaaat ggcgctttat tcttgaaaat atttttgatt
     5041 tattggctat tctcccttta aatgctattt ttacagtgtt tcgattaggg cgtatctttc
     5101 gtttagctaa actaacaaaa ttactgaaac tgactcgttt acttagaata attggactta
     5161 caggtaaatt agaaagaaaa attagtagat tcttacggac aaatggcttg atttatatct
     5221 tgtatgttaa tatctttatc gtgctagtag gaagttcaat tttatctgta gttgaagaaa
     5281 aatccttctc agatagtctt tggtgggctc ttgtaacggt aacgactgtt ggatatgggg
     5341 atattgttcc tgcttccatt tttgggaaat ggctagcagt tttactgatg cttgtcggta
     5401 ttggaacaat agggatgtta acaagtgctt tgacgaactt ttttgtaaag gataatccag
     5461 atgaacagat aaaacttgat aaactccaag atgaattatc tagtcagaga atattactag
     5521 agaaacaatc taaaaagata gaagaactac ataaaatgat acaagatttg attgaaaaaa
     5581 cataataaaa ataaagatga gaaaaagttt gaactgagga gaaatcaagt aagttttgac
     5641 acaatagaac gcatattatc aggtataaaa acttgatact atgcgtttta ttgtgggaag
     5701 atgtataatg gattgaaata agatatgaac aaatcgatta ggaatttaaa gcattttata
     5761 acaacgtttt agagtaacgg ggtactattt catataatac aggaaaaaac aactccctga
     5821 tgattcaagg agttgtctat agttaaatta gtttttagaa gcttcttgga attctggatt
     5881 tttccatgct tcgtcaataa tagcttgcaa ttctttagca gatgcttgca ttttttgagt
     5941 ttctgcatcg ttcaatggaa tgtttactgg acgaacgata ccatgtgcac caacaacagc
     6001 tggttgaccg ataaagacat tctcaactcc gtattgacct tcttggaata ctgaaagtgg
     6061 aagtactgcg ttttcatcgt caaggattgc tttagtgata cgagcaaggg ctactgcgat
     6121 accgtagtat gttgcacctt ttttgttgat gattgtgtag gctgcatcac gaacaccttc
     6181 gaacaattca atcaattcag cttcttgaac attttgagtg tctttaagga attcttcaag
     6241 gtttacacca gcgatgttag cgtgtgacca aacagcgaac tcagagtcac cgtgttcacc
     6301 catgatgtag gcgtgcactg aacgagcatc cacatccaat ttttcagcaa gtgcttgacg
     6361 gaaacgagct gagtcaagtg aagtacctga accgataacg cgttctttag ggaaaccaga
     6421 gaatttccaa gttgagtaag tcaaaacgtc aactgggtta gcagcaacaa ggaagatacc
     6481 tttgaaacca gattcaacaa cttgagttac gattgatttg ttgatagcaa ggtttttacc
     6541 tacaaggtca agacgagttt cacctggttt ttgaggtgca cctgcagtga tcacaacaag
     6601 gtcagcgtct gcacagtcag agtattgagc tgcatagatt tttttaggtg aagtgaaggc
     6661 aagggcgtga ctaaggtcaa gcgcatcacc aacagctttt tcatgcaatt gtggaatttc
     6721 gataattcca agctcttgtg caattccttg gttaacaagt gcaaaagcat aagatgaacc
     6781 tacagcacca tcaccgacaa ggataacttt tttgtgttgt ttagttgaag tcattgtttt
     6841 aaacatctcc ttaattttat taggggattt tccctagaca acttcattct atcactttta
     6901 aaaaactttg tcacgaatat gccttatagt tctcgatgta aacgttttag tggtttagag
     6961 gctgaaatag atgggaattt atggtataat gttgttactt actaattgtg aaatgaggca
     7021 tttattaatg caggataaaa atttagtgaa tgtcaatctg acaaaggaga tgaaggcaag
     7081 ttttatcgac tacgccatga gtgttatcgt agcgcgagct cttcctgatg ttcgagatgg
     7141 cttaaaacct gttcaccgtc gcattctcta cggaatgaat gaattgggtg tgaccccaga
     7201 caaaccccat aaaaaatctg ctcgtattac aggggatgtc atgggtaaat atcacccaca
     7261 cggggattcc tctatttatg aagccatggt ccgtatggct caatggtgga gctaccgtta
     7321 catgcttgta gatggtcatg ggaattttgg ttccatggat ggagatagtg ctgccgctca
     7381 acgttatacc gaggcacgta tgagcaagat tgctctggaa atgcttcgtg atatcaacaa
     7441 aaatacagtt gatttcgttg ataactatga tgccaatgaa cgggaaccct tggtcttgcc
     7501 agcgcgtttt ccaaaccttt tggttaatgg agcaactggt atcgcggttg ggatggcaac
     7561 caatattcca cctcataatc tgggtgaaac cattgatgca gtgaagttgg tcatggataa
     7621 tcctgaagtg actaccaagg acttgatgga agtcttgcct ggaccagatt ttccaactgg
     7681 tgctcttgtc atggggaaat caggtatcca taaggcttat gaaacaggta aaggttcgat
     7741 tgtcctacgt tctcgtacag agattgaaac gactaagact ggtcgtgagc gtatcgttgt
     7801 aacagaattt ccttacatgg tcaataaaac caaggtgcat gagcatattg ttcgcttggt
     7861 tcaggaaaaa cgcattgagg gtatcacagc agtacgtgat gagtcaaacc gtgaaggtgt
     7921 tcgatttgtt attgaagtca agcgcgacgc ctcagccaat gttattctca ataacctctt
     7981 caaaatgacc caaatgcaaa ccaattttgg tttcaatatg ctcgctatcc aaaatggtat
     8041 accgaaaatt ttgtctcttc gtcagatttt ggatgcttat atcgagcacc aaaaagaagt
     8101 ggttgttcgt cgtacacgtt ttgataagga aaaagcggaa gcgcgcgctc atatcttaga
     8161 aggtctcttg attgcgctag accatatcga cgaagtgatt cgtatcatcc gtgctagtga
     8221 aacggatgcg gaagctcaag ctgagttgat gagcaagttt aagctttctg aacgtcaaag
     8281 tcaagctatc cttgatatgc gtcttcgtcg tttgacaggt ttggaacgcg ataagattca
     8341 atctgagtat gatgacctct tggctctgat tgcggattta gcagatattc ttgctaagcc
     8401 tgaacgtgtt tctcaaatta tcaaagacga attggatgaa gttaaacgta aattttctga
     8461 taaacgccgt acagagttga tggttggaca gatcttgagt ctcgaggatg aggacttgat
     8521 tgaagaatcg gatgtcttga ttaccctttc taacagaggc tacattaagc gtttggatca
     8581 ggacgagttc actgctcaaa aacgtggggg tcgtggtgtc caaggaacgg gagtgaaaga
     8641 tgatgacttt gttcgtgagt tagtgtcaac tagcacacat gatcatctgc tcttcttcac
     8701 aaacaaggga cgtgtctatc gtcttaaagg ttatgaaatt cctgagtatg gtcggactgc
     8761 caaagggcta ccagtagtca atctcttgaa attggatgaa gacgaaagta ttcagacggt
     8821 tatcaatgtt gagtctgatc gcagtgatga tgcttatctc ttctttacaa cccgtcacgg
     8881 tattgtgaag agaactagtg ttaaggagtt tgccaatatt cgtcaaaatg gtctcaaagc
     8941 gctgaattta aaggatgaag atgagttaat caatgtcttg ttggcagaag gagatatgga
     9001 tattatcatt ggtaccaagt ttggttatgc agttcgcttt aatcaatcag ccgttcgtgg
     9061 tatgagccgt atcgccactg gtgtgaaagg tgttaacctt cgtgaaggag acacagttgt
     9121 tggtgccagc ttgattactg atcaagatga ggttcttatt atcacagaaa aaggatatgg
     9181 taagcgtaca gtcgctactg aatacccaac aaaaggtcgt ggtggtaagg gaatgcagac
     9241 agctaaaatt accgaaaaaa atggcttgct ggccggtctt atgactgttc aaggggatga
     9301 ggatttgatg attatcactg atacaggtgt catgattcga accaatcttg ccaatatttc
     9361 acaaacagga cgtgcaacta tgggagttaa agtaatgcgc ctggatcaag atgctcagat
     9421 agtgactttc acaacggttg cggtggcaga aaaagaagaa gttgggacag aaaacgaaac
     9481 agaaggtgaa gcataatgtc tcaaaaaaat aataaaaaga aaaacaagcg aaaaaatctg
     9541 ctgacaaata tcctagcagg atttctgata ttactgtcac tggctttgat ttttaatact
     9601 caaattcgaa atattttcat agtctggaat accaataagt atcaagttag ccaggtatca
     9661 aaagaaaaat tagaagaaaa tcaggataca gaaggcaatt ttgactttga ttctgtcaaa
     9721 gctatctctt cggaagctgt tctaacttct caatgggatg ctcaaaaatt accagttatt
     9781 gggggaattg caattcctga attggaaatg aatttgccga tttttaaagg acttgataat
     9841 gttaatctct tctacggagc tggtacaatg aaacgcgagc aagtaatggg agaaggaaat
     9901 tatagtctag ctagtcacca tatctttggt gttgataatg ctaataaaat gttattttct
     9961 cctttagata atgctaaaaa tggcatgaag atttatctaa ccgataaaaa taaagtttat
    10021 acttatgaaa tacgtgaagt caaacgtgtg acaccggatc gtgttgatga agttgatgat
    10081 agagatgggg tcaatgaaat cacattagta acctgtgaag accttgctgc tacagaacgt
    10141 attattgtca aaggtgattt gaaagaaaca aaagattatt cacaaacatc tgatgaaatc
    10201 ctaacagctt tcaatcaacc atataaacaa ttttattaat acaaatcagt gaaatcatgg
    10261 atttcactga ttttttaaga ttttcataaa aatatagtaa aatgaaataa gaataggacg
    10321 aatcg
//
LOCUS       DBERMR010000070        10306 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.070, whole genome shotgun sequence.
ACCESSION   DBERMR010000070 DBERMR010000000
VERSION     DBERMR010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10306
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.070"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(346..738)
                     /gene="rpsI"
                     /locus_tag="ACL8A1_001946"
     CDS             complement(346..738)
                     /gene="rpsI"
                     /locus_tag="ACL8A1_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HGS1202654.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(757..1203)
                     /gene="rplM"
                     /locus_tag="ACL8A1_001947"
     CDS             complement(757..1203)
                     /gene="rplM"
                     /locus_tag="ACL8A1_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HGS1202655.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            1559..1876
                     /locus_tag="ACL8A1_001948"
     CDS             1559..1876
                     /locus_tag="ACL8A1_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HGS1202656.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            complement(1921..2733)
                     /gene="folK"
                     /locus_tag="ACL8A1_001949"
     CDS             complement(1921..2733)
                     /gene="folK"
                     /locus_tag="ACL8A1_001949"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HGS1202657.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIHILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(2776..3330)
                     /gene="folE"
                     /locus_tag="ACL8A1_001950"
     CDS             complement(2776..3330)
                     /gene="folE"
                     /locus_tag="ACL8A1_001950"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HGS1202658.1"
                     /translation="MDTQKIEAAVKMIIEAVGEDANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            complement(3311..4633)
                     /locus_tag="ACL8A1_001951"
     CDS             complement(3311..4633)
                     /locus_tag="ACL8A1_001951"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HGS1202659.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFNSPYLIHYTDQISINGESISEARLEALMADY
                     QSLMGGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLESIAEQKAGIIKQGMPLVTGRIAPEALAVIDRIAEEKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNVTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            complement(4635..5609)
                     /gene="folP"
                     /locus_tag="ACL8A1_001952"
     CDS             complement(4635..5609)
                     /gene="folP"
                     /locus_tag="ACL8A1_001952"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HGS1202660.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGADLVNDITGLMGDEKMAHVVAEARAQVVIMFNPVMARPQHPSSLI
                     FPHFGFGQAFTEEELADFETLPIEELMEAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEENGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            complement(5704..6411)
                     /locus_tag="ACL8A1_001953"
     CDS             complement(5704..6411)
                     /locus_tag="ACL8A1_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGS1202661.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVTGISIVVLVLFIIGARKTKLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            complement(6464..7882)
                     /locus_tag="ACL8A1_001954"
     CDS             complement(6464..7882)
                     /locus_tag="ACL8A1_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HGS1202662.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTVMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            8072..8884
                     /locus_tag="ACL8A1_001955"
     CDS             8072..8884
                     /locus_tag="ACL8A1_001955"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGS1202663.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKRTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKEMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            9223..10242
                     /gene="adhP"
                     /locus_tag="ACL8A1_001956"
     CDS             9223..10242
                     /gene="adhP"
                     /locus_tag="ACL8A1_001956"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HGS1202664.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSETMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
BASE COUNT         3043 a         2337 c         1991 g         2935 t
ORIGIN      
        1 agtagattga aactagaata gtacacctct acttctaaaa cattgttaga attcgatttg
       61 actatcctca gtttgtccta ttcttatttc accttactat aaaaataagg taaacttttc
      121 aaaaaaacac gaaaaaagca cccataaggt aaacttttga gtgctttgaa acgttgatat
      181 aatcgtattg attaacgttt tgaggcaact gaccttgtca ggttgcagcc acatttgtaa
      241 gcgactaaag tcgcaacaac tgtgtcaatt gcaccaattt agtaagaaag tataaaaaag
      301 aacaccccga aaggtgctct gtataagtat agtaattctt tcgaattaac gtttactaaa
      361 ttgtgatgct ttacgagctt tcttaagacc tggtttctta cgttcaactt tacgtgagtc
      421 acgtgtaaga agtcctgcgc gtttcaatga atcgcggaag tctgggtcta cttgaagaag
      481 ggcacgagcg ataccgtgac ggatagctcc tgattgacca gcgtatccac cacctacaac
      541 gttaacgaaa acgtcgtatg aacctacagt tgaagtaact gcgaatggtt ggttgatgac
      601 aagacgaagg tcagcgtgtg ggatgtactc ttcaacatct tttttgttaa cagtgatttt
      661 accagttcct ggaacaaggc gaacgcgtgc aacagcgttt ttacgacgtc cagtacctgc
      721 atattgtgct tgtgacatac tttattgttc ctttccttag ataagtcctg aaatgtcaag
      781 aacttctggt tgttgtgcag cgtgagtgtg ctcagctcca acaaatactt tcaacttcat
      841 accttgagcg cgtccaagtg tattgtgtgg aagcatacct ttaactgatt tctcaatcaa
      901 acgtactgca tttttagaac gaagttcacc tgcagagatt tgtttcaatc cacctgggtg
      961 gtttgagtga gtgtagtaga ttttatcagt tgctttttta ccagtcaatt taactttttc
     1021 agcattgata acaatcacga agtcacctgt atcagtgtgt ggtgtaaatg ttggtttgtt
     1081 ttttccgcga agtacgctag caactactgc agaaagacgt ccaagtggta catcagttgc
     1141 gtcaactacg taccatttac gttcaacttg gcctggttta gccataaatg ttgttttgtt
     1201 catgatttct cctatatata gttcgatttt tgtttacggt gatgggtgtt ccttcccatc
     1261 gaaaagtatt tggaaggttc cggggccttt caaatggggt aaacaatacc gcctactatc
     1321 ataccaaatt ttacctctaa aagtcaatag atatgaaaaa tatttttcgg taacttctca
     1381 aagtaactga aagttatata atcaagttaa tttgggaatt tacctattaa cttcggtaga
     1441 tttttaggaa ttttttccct atttctccct gtaactaaaa tccttttcaa aaaatcacct
     1501 tttcttgatt tttaatccta tttgctgtat gataagggaa aagaaagggg acagagatat
     1561 ggcttttacc aatacccaca tgcgatctgc tagttttggc attgttacca gcttgcctga
     1621 tgacatcatt gactcttttt ggtatatcat cgaccatttc ttaaaaaatg tctttgaatt
     1681 ggaagaagaa ctcgagtttc aattgcttaa taaccaagga aagattacct tccacttttc
     1741 aagtcaacac ctccctacag ccattgattt tgactttaac catcctttcg accctcgtta
     1801 tcccccaaga gtactggttt tagacatgga cggtagagaa actatcctcc tcccagaaga
     1861 aaatgaccta ttttaaaaac tctagccttc agttgcaagt aactgaaaac tagagttttt
     1921 ctattttttc aaagcatcat acaagttgcg gatcggttgt tttaatatcg gatggataaa
     1981 atgaggcgca atttcctgta aggactcaag gacaaaaagg cgttccgcta tgtaaggatg
     2041 aggcaatatg aggtcgtctg tataaaggat ctggtcctcc acaaagagca agtccaaatc
     2101 aatcaaacga ggtccccaat gcacttctct cacccgtccc agctctgact caatggctaa
     2161 caaggtttct aacaagtctt gtgctggtag ccaggtttcc acctcaacca cttgattggc
     2221 aaagctatcc tgttccactc caccccaagg ctccgtcgct aagacactgg actctttgag
     2281 aatatggatg ccacgagctc gcagtttgtc aatggcttgc ttcaagtttg cttgcttatc
     2341 tcccatattg cttcctaggg cgataaaggc tcgttgcttg cggcgatgaa tggttaccga
     2401 gcaagtatct agtgacaaat gcaccggtgc ccaaggtttt ttcagttcca acttcatttc
     2461 ttggacaaga ggataagact caaaggtacg ttccaccagt ttataggcta ccgtttcaat
     2521 caaatcttca ctcgtttcct gaaaccaagt cgtccactgc tgacacaatt ctccgtaatg
     2581 gacagaggct gttaaatcca agtctgtagc tgccttggtc atatcatagg ataggatggc
     2641 tgaaacgaca aatttctgcc ccaattcttt ctcactagga aaaagaccat gataggcaaa
     2701 catttccaaa tccttaatct gcagttgatc cataatgatt cctttctaga aaaatccgct
     2761 tgaagcggat tctttttata gccccattaa acgataagct tggtcgcgga ggtccttatc
     2821 tgtttcaaat agaccacgag ctactgtcgt caaggttgca gtgcctggtt ttctaacacc
     2881 acgcatactc atacacatat gttccgcctc aatgacaaca aaggctcctt tagcacctag
     2941 atagtccatc aaggcatcgg ccacttcgat attcaaacgt tcttgaattt gtggtttttt
     3001 cgaataaact tcaaccgtac gggctagctt agacaagcct gccacacgac catctggaat
     3061 gtaggcaatg tgcgctctac cataaaatgg caagaagtgg tgttcacaca tggtatggaa
     3121 aaagatatcc ttttctacca ccatattatc gtcaataatt tcaaaggatt ttgacaaatg
     3181 ttcctctgct gtttgaccaa gacctgaaaa aatctcttga tacatacggg ctacacgagc
     3241 aggtgtttcc tgcaaaccct cgcgattagc gtcctctcct acagcctcga taatcatttt
     3301 tacagctgct tcaatctttt gtgtatccat tctcgttctt cctctccatc agataggccc
     3361 tcacttggct caagaaatac aaggaacctg tgacaatcct aactgtttgt ttctcttctt
     3421 ttttatctgt cacattctgc tctagaaaat catgccaatc ttggtagctg agatttctag
     3481 acttagctgc ctctttcagc acgctttcat ccgttgcccg actatccgca aaatgtgtta
     3541 gagtaagctc ggtatctggc atggctccca gcaagtccaa catatcctcc aaggccttgg
     3601 ttttgataca agtgaagagg atttccttat gataatccgc aaaacgttct tgcaaggtta
     3661 ccaacaaagc cttgatagca tggggattat gggctccatc caaaatcatc aagggatctc
     3721 ttgacacgat ttccaaacgc cctggccaac ttgtttcttc caaggcttga ccaagaaagt
     3781 cattgcttgc tagctctcga ccatcttctt gacaaaaagt atcaagtaaa gctatggcca
     3841 tcccagcatt ctctatttgg tacaaaccaa gcaggctagt ctggaagcga ccttgtctga
     3901 cagcacttgt atagtcaaag acttcccctg tcaccacact ttcttgatga cgaacctgat
     3961 aatctgtccc gtaggcaagt ctcggcgcat ctttctcttc cgcaatgcgg tcaatcacag
     4021 ctaaggcttc tggagcaata cgccctgtta ccaagggcat cccttgtttg ataatacctg
     4081 ccttctgctc tgctatggac tccaaggtgt caccaagtag ggccacatga tccaagccaa
     4141 tagttgtaat tcctgtcaaa atgggctgac agacattggt actatccaaa agtccaccca
     4201 tgccaacttc catgatggcc acatctactt gctctgaggc aaagtagtca taggccaggg
     4261 ctgtgataat ctcaaactcg gttgtgccct gtaaattggc gaccgcttct ccccccatca
     4321 aagactgata gtctgccatg agagcttcta gcctcgcttc tgagatcgat tccccattga
     4381 tgctaatctg gtctgtgtaa tgaatgagat agggcgagtt aaacacgcca actctcagcc
     4441 ctagcttttc tagcatcttt ttcaaaaaag caatagtcga gcccttgccg ttagtccctc
     4501 cgatatggag gaccttgagt ttgagatggg gattgccacg caaagctaac agttccacca
     4561 ttcgttccaa accaaaatgc ggttgatccg tccggtagtt agcaatccac tgattgtttt
     4621 caatttcttt catcttattt atattgtttt aaatctaaat tttccgcttc atcagccaga
     4681 cgaatggcag aggcaatttc aactgccatc ctgtgactag ctacgtcatg cacgcgcacc
     4741 acttctacac cctgtctcgc agcgatacta gttacatgag ccgaagccgt gtcccgattt
     4801 cggaaaccaa gctctgtctc aggattgact tcaaaaccat tctcctctag gatattgatg
     4861 acaaatcgct tgcgcgacac tccgagaaag attggatagc ccttctgatg tagtttatcc
     4921 aggtcccgta aaagaagcag attttctttc ttggtcagac caaagccaat tcctggatcc
     4981 aacaggatat tttctggtgc aataccagct tctgccgctc tcgctagtgc tcgttcaaag
     5041 aaagcctcca tcaattcttc gattggcaat gtttcaaagt cagctaactc ttcctctgta
     5101 aaagcttgac caaaaccaaa atgagggaag atgagcgagc tagggtgctg aggtcgcgcc
     5161 ataactggat taaacatgat gaccacttgc gctctcgctt cagctaccac atgagccatt
     5221 ttctcatcac ccataagacc agtgatatca ttgactagat cggcacccgc agccaaagca
     5281 gcctctgcta cttgactctt ccaagtatca atagagatga ggacatcact ttccttgcga
     5341 atcgctttga tcactggaac aacacgctgg atttcctctt ctatctcaac atagctactt
     5401 cccggccgag tcgattctcc gccgatatct agcatactgg ctccttctgc tatcaattta
     5461 cgagcctgct ggagcgcctg ctcaagagca aaaaattgac caccgtccga aaaggagtct
     5521 ggggttacat tgataattcc gcaaataact gtctttgcat gattggcttt acttgacata
     5581 tcggtcactc cctcaaggat tttcatcata ttatttttct attttaccat aaaaagaaaa
     5641 agatggacac gattgcattc atctttccct agtagaaaga ggtcggttcc tacataaaaa
     5701 cttttaaaca gaaattccta atgtccgact cataatcacc acaagagcca acaaacagaa
     5761 agcaatccca ttaacaatca tgtgaagcaa gatcgacatt tccaaacgtt gggtcttgta
     5821 ggccgtccaa gatagaactg tcgacatacc tccataaatc aataaagaag gtaaattact
     5881 tggttgatgc aataaagcaa acacaatcgt accgactaca aatcccaagt tctccttgcc
     5941 tcggaaaatc tttttaggaa caatcccacg gcacaagatt tcctcacaaa tcggagcaag
     6001 caaggctagc aagaagaaac tggaaatcaa cgaactattt tgaaccatat cgttaatctg
     6061 agactggtta gctgttgtcg tctcatttga cagttgcaac aagatagaac caagtatatt
     6121 tgacccgaca ataactaaat aactcaatcc caaacgtgcc aaatctttag ctctaaaaaa
     6181 agaaaaatta aaactagcta acttggtttt acgagctcca ataataaata gcaccagaac
     6241 cacaatagaa ataccagtaa ctatcagccc tgactgtagc agggctactt cttttaaagt
     6301 caaaatagag gtaaccacta ggggaacctg atataaaagg acggcaagta aaaagaccaa
     6361 aatccagcct gcacgattca acaattcttt ccacatattt ttctctttca tcacctatct
     6421 cctttgtatt aaaaaagggg ggaaatcccc ctgggtcatt ctattataag gccatgctga
     6481 tgtagttaag gataaacaag gcatccaaaa tccaaatcat ggcatgaaca tctttaactt
     6541 gacctttgac aagcttagtc aaagtgtaag tcaagaaacc aactgcaatc ccttgagtga
     6601 tagagtagct gaatcccata aagatagatg tgaagaaggc aggaactgct tcagacatat
     6661 catcccaatg gatatttttc aagctaccaa gcatcataat cccaacgata atcaagattg
     6721 gagctgtagc cgctgttggt acgatcgcta gaagtgggct aaagaagctt gaaatcgcaa
     6781 aacagatagc tacaaccaag gctgtcaaac cagtacgtcc acctgcaccg ataccagcag
     6841 cagactcaac ataagtcgtt acgtttgaag tacctgcaat ggcaccgact gtcgttccaa
     6901 tcaaatcaga gtaaagagcc ttatccaatt tggctgattg gtgattttca ccatttgtcg
     6961 ctacaatacc aactttttca cctgtaccga tcaaggtacc aattgtgtca aaaatatctg
     7021 tcaatgagaa ggcaagaatg gccatcagag tttcaggcaa gcgagctgta tctgaaacca
     7081 aagctcccaa tccttctgaa ccaagagctg caccaaagat tgtcttcaaa tcttcaaaag
     7141 ctgcaccaac atggttatta gcaaaatcga tactagacaa atcaaccaaa ccaactgcaa
     7201 tagcaagaac agttgttgtc aagatagaga gaataattcc ccctttaatc cctttgatga
     7261 caaagaagat agtaatggca agtcctgcaa gagccactaa aacagctgga ttattaaagc
     7321 tgaccaatcc tggaactgct gaagagtttg ctgcaatcgt tgcttgagct ttgtcagccc
     7381 cttctcctac aacagtatag ttgcctggat caatcgtgaa tttcaaaagt ccagcattct
     7441 taatccctac ataggcaagg aagacaccga taccagctga aatagctgag cgaagagcat
     7501 tgggaatcga ttcaatgatc attttacgaa catttgtcaa ggtaataatc aatgaaataa
     7561 tcccacagat gaagaccata gctagggctt cttgccaaga ataaccaagc ccgaatacaa
     7621 ctgtaaaggt aaagaaggca ttgagtccca tacctggcgc ttgggcataa ggtaagttag
     7681 cataaaaagc catcatcacg gtacccgcta ctgcaccaat aatcgtcgct aggaagacgc
     7741 cctgagcagg cattcctgtt tgtgaaagta tttgtgggtt tacaaagaga atatagctca
     7801 ttgcaaagaa agttgttaaa ccagcgagaa cctctgtacg aacgtctgta ccgttctctt
     7861 ttagtttaaa taatttgtcc atcatcaatc tccttttaat ttttacgaac aataatagaa
     7921 ttatatcatt tatcattcac tttttcaatg tctttgtttg atttgtttaa gaaattaatc
     7981 caatttcatt ttttgtttgg aatctgcttt tctgcctgct ttctgttata atagaagaca
     8041 tcttatctga aaacacacta gaaaggtcca tatgacgaaa aaaattattg cagttgacct
     8101 ggatggaacc ctgctcaact cagacagtca aatttctgac tttaccaaaa gaaccatcaa
     8161 aaaagttgct gaaaaaggcc atcaggttat tattacgaca ggtcgccctt accgtatgtc
     8221 aaaagatttt taccgtgaac tgggcttaga cactcctatg attaacttca acggatccct
     8281 tactcattta ccagaccaag tttgggattt tgaaaagtgt ttgactgtag acaaaaaata
     8341 tctgctagat atggttcaac gttcagagga cattcaagcc gattttatcg ctggagaata
     8401 tcgtaaaaaa ttctacatta caaatcccaa tgaagaaatt gccaatccca aactatttgg
     8461 tgtagaagct ttccagcctg aagatcaatt ccagcctgaa ttggtgacca aggaccctaa
     8521 ctgtatcctc ttgcagacca gagccagtga caaatatgcc ttggcaaaag aaatgaacgc
     8581 cttctaccag catcaacttt ctatcaatac ctggggaggt ccgctcaata tccttgaatg
     8641 taccccaaaa ggtgtcaaca aggcctttgc tttggactac ttgctcaaga taatgaatcg
     8701 tgacaaaaaa gatttgattg cctttggaga tgaacacaat gataccgaaa tgctcgcttt
     8761 tgctgggaag ggttatgcca tgaaaaatgc caatccagag ctactccctt atgcagatga
     8821 gcaaatttcc cttaccaacg accaagatgg ggttgccaaa accctacaag acttattctt
     8881 ataacctata ctgatactca atgagaatca aagagcaaac taggaagcta gccgtaggtt
     8941 gctcaaagca ctgctttgag gttgtagatg aagctgacgt ggtttgaaga gattttcgaa
     9001 gagtatgacc agcttgcaag ctggtctttg tgctcttttc acagtctcat caaccagtta
     9061 atcgattgtt ctactttttg tctccaaaac cttggaaaag gctttctttt gtgatatact
     9121 tcacatataa atacaagaga gctcgtcaca ctcgactctg ttgacaaaaa atcaatccag
     9181 tcccgttgtc cctgttctcg gtcaatatca aggaggatag ctatgaaagc tgttgttgta
     9241 aatccagaaa gcactggtgt tgctattgaa gaaaaagtac tccgtccact tgaaactggg
     9301 gaagcacttg tagaagttga atactgtggt gtttgccaca ccgacctcca cgttgctcat
     9361 ggtgactttg gtcaagtccc aggacgtgtt cttgggcacg aaggtatcgg tatcgttaaa
     9421 gagattgctc cagatgtgaa aagccttaaa gtcggtgacc gcgtcagcgt tgcttggttc
     9481 tttgaaggat gtggcacttg cgaatactgt acaactggtc gcgaaaccct ttgccgtaca
     9541 gtgaaaaatg ctggctactc agtagacggt ggtatggctg aacaatgtat cgtaactgct
     9601 gactatgctg tcaaagttcc tgacggactt gatccagccc aagcttcttc tatcacatgt
     9661 gctggagtaa caacctataa agctatcaaa gaagcaaaag ttgaaccagg ccaatgggtt
     9721 gttctttacg gtgctggtgg acttggtaac ctcgctgttc aatacgctaa aaaagtattc
     9781 aatgctcatg ttatcgcagt cgatatcaac aatgacaaac ttgcccttgc aaaagaagta
     9841 ggcgctgaca ttgtgattaa cggcctcgaa gttgaagatg tagctggact cattaaagaa
     9901 aaaactgatg gaggagctca ttcagctgtc gtaactgctg tgtctaaagt tgccttcaac
     9961 caggctgttg actccattcg tgctggtggt cgcgtcgtcg ctgttggtct tccttctgaa
    10021 acgatggaac tcagcatcgt taaaacagtc ctcgatggaa tccaagtcat cggttctctt
    10081 gtcggaactc gtaaagactt agaagaagcc ttccaatttg gtgcagaagg tctggtagtc
    10141 ccagttgttc aaaaacgtcc agtagaagat gctgttgcca ttttcgacga aatggaaaaa
    10201 ggccaaatcc aaggacgtat ggtactcgac ttcacccact aatctaatag aaacctattt
    10261 taaaagttgg aagcttcttc caactttttt atattaaatt ttaaat
//
LOCUS       DBERMR010000071        10290 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.071, whole genome shotgun sequence.
ACCESSION   DBERMR010000071 DBERMR010000000
VERSION     DBERMR010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10290
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.071"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            103..272
                     /locus_tag="ACL8A1_001957"
                     /pseudo
     CDS             103..272
                     /locus_tag="ACL8A1_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            472..3747
                     /gene="rexB"
                     /locus_tag="ACL8A1_001958"
     CDS             472..3747
                     /gene="rexB"
                     /locus_tag="ACL8A1_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HGS1202665.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPVKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRTDLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQITLVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWNVFLKEGVVTKQLQDLTATMEAIEQERQAEVWKAFCHVL
                     EQFATVFAGSQISLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKIAQNTSLLTDEERQNINQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLVLSAPSLFNESESKESAYLQELVHLGFSRKEKRMNHKRLSKEDMGSYHSLLSSL
                     VAYHQQGETSDTEQDLTFVKVLARVMGKKLDQQGLENPALPTSPSSKQLTKDTLQALY
                     PAEQEFYLSTSGLTEFYRNQYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PDEDSFDNRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPVIE
                     TIKEEANFGGKEQAFIQLDNGRSVFVRGKVDRIDRLKADGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSHLGEFYNKNKANQLTDEEFQLLLDYNAHLYKKAAEKILEGQFAINPYT
                     ENGRSIAPCVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWLEKIREE
                     LNR"
     gene            3744..7394
                     /gene="addA"
                     /locus_tag="ACL8A1_001959"
     CDS             3744..7394
                     /gene="addA"
                     /locus_tag="ACL8A1_001959"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HGS1202666.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVELKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKETFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SDSFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQESDSQAYQAVLAHVVAISKEKNGRALTNASRKADLKPLADTYNEERKTQ
                     FAKLGQLSDQIAILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNKHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVVVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLESKEYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTALGQEIFANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            7564..8742
                     /locus_tag="ACL8A1_001960"
     CDS             7564..8742
                     /locus_tag="ACL8A1_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202667.1"
                     /translation="MTLPVRKSLHDAVLQASEADTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNVIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
     gene            8951..>10290
                     /locus_tag="ACL8A1_001961"
     CDS             8951..>10290
                     /locus_tag="ACL8A1_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HGS1202668.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGIGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPSPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGAIVEPAIQPELPEAVVSDKGEPEVQPTLPEAVVT"
BASE COUNT         3275 a         2073 c         2170 g         2772 t
ORIGIN      
        1 gctcacaata agagagaact tttttacgaa aatctattga atatgccata aaaagattat
       61 accacattgt gtactatttt tggttcattt tactatagtt caatcattct atcaagaata
      121 ttgaatatat aaaggcacgt aatattaaaa cttcttctga tatcgaatta gagtccgatg
      181 ttgatggcga taaatcagac aatctacccg tcgacattaa gatactggga caacacattg
      241 aagtgtattc cgtacctaag aaacaatcat aaaactcaaa gccttgttac tgaggctttt
      301 ttattatgtg attttcagta gccagactta ttctatctaa tacaagtaaa gctaagtatt
      361 taagatttag cagataatga agaaaagaaa ggatggccta aatattttaa cagatttacg
      421 aggctaatca tggtataata tagggtgatg aaaaattcca acgaggctga gatgaaatta
      481 ctttatactg atattcgaac ttctttgaca gaaattctaa caagagaggc agaagagcta
      541 gttgcagctg gcaagcgggt cttctacatt gcccccaact ctctttcttt tgaaaaggaa
      601 cgcgccgtgc tggaatgctt gtcccagcag gcttcttttt cgattaccgt cacgcgcttt
      661 gctcaaatgg ctcgttatct ggtcctgaat gatttaccag ttaaaactac tcttgatgat
      721 atcggtcttg ggttggcctt ttacaaatgc cttgccgaac tcgatcccaa ggacttgcgt
      781 gtttatggcg ctattaagca ggatcctcaa ttgatccagc agttaattga gctttaccat
      841 gagatgacca aatctcagat gagttttttg gacttggaga atttaacaga tgaggataag
      901 aggacggatt tactcttgat ttttgagaaa gtaacagcct atcttaatca aggtcagtta
      961 gcccagggaa gtcagttgtc ccatttgatt gaggctattg agaatgacaa ggtaagtagt
     1021 gattttaatc aaatcacctt ggtcattgac ggctttactc gtttttctgc tgaggaagag
     1081 cgggttgtgg acttacttca cggcaaaggt gttgagattg ttatcggggc ttatgctagt
     1141 aagaaagcct ataccagtcc ttttagcgag ggcaatctct accaagccag cgtaaaattt
     1201 ctccatcatc tggcttctaa ataccaaacg cctgctcagg actgttctca aactcatgag
     1261 aagatggata gttttgacaa ggcctctcgt ttgttggagt cttcttatga cttttcagaa
     1321 ctcgctttgg atgtcgatga gaaagaccgt gaaaatttac aaatctggtc ttgtttgacg
     1381 caaaaggagg agttggagct agtagcccgt agcattcgtc agaaattaca tgagaactca
     1441 gacctgagct acaagcattt tcgtattctc ttgggggatg tagcttctta ccagttatct
     1501 ctcaaaacca tttttgacca gtatcagatt cctttttatc ttggtagaag cgaagccatg
     1561 gctcatcatc ccttgactca gtttgtcgag tctattttag ctttaaaacg ttaccgtttt
     1621 cgtcaggagg atttgattaa tcttcttaga actgatttgt atactgacct cagtcagtct
     1681 gatattgatg cttttgagca atatatccgc tatcttggta tcaatggctt gccagccttt
     1741 cagcaaacct tcaccaaatc ccaccatgga aaatttaatc ttgagcgttt gaatgtcctc
     1801 cgtctgagaa ttttagcacc tcttgaaacc ctctttgcta gccgaaaaca aaaggccgaa
     1861 aatctcttgc aaaagtggaa tgtctttcta aaagaaggag tggtgaccaa gcagttacaa
     1921 gatttgacag ccactatgga agctatagaa caggaaagac aagccgaggt ttggaaggca
     1981 ttctgtcatg ttttagaaca atttgcgact gtttttgctg gttcacagat tagtctagag
     2041 gacttcctag ccttgctcca ttctggaatg agtttgtctc agtatcgcac tattccagcg
     2101 acagtggaca ccgttctggt gcagagttac gatttgattg cgcctttgac tgctgacttt
     2161 gtctacgcca ttgggctaac tcaggacaat ttaccaaaaa tcgcgcaaaa taccagtctt
     2221 ttgacagatg aagaaagaca aaacataaac caagcgaccg aagaaggcgt tcaattactg
     2281 attgccagca gtgaaaatct caagaaaaat cgctacacta tgctttcttt ggtcaattct
     2341 gcccgtaagc aattagtttt gtcggctcca agccttttta atgaaagtga aagcaaggag
     2401 tcagcctatc ttcaagagtt ggtccatttg ggatttagtc gaaaagagaa gaggatgaat
     2461 cacaaaaggc tgtctaagga agatatggga tcctatcaca gtcttttgtc cagtctggtt
     2521 gcctatcacc agcagggcga gacgagtgat actgagcaag atttgacctt tgtcaaggtt
     2581 ctggcgcgtg tgatgggtaa aaaactagac cagcaaggtt tggaaaatcc tgcccttcca
     2641 accagtccaa gcagtaagca gttgaccaaa gataccttac aagctctcta tcctgctgaa
     2701 caggaatttt acctatctac gtccggtttg accgagtttt acagaaacca atacagttat
     2761 ttcctccgct acgtgttagg cttgcaggag gaattacgtt tgcgtccgga tgctcgcagt
     2821 catggaaatt tcttgcaccg catttttgaa cgtgccttgc agttgcctga tgaagattcc
     2881 tttgacaacc gtctagaaca agctattcaa gaaactagtc aagaacgcga atttgaagct
     2941 atttatcaag aaagtttgga agcccagttt accaaagaag ttttgcttga tgttgcacgg
     3001 acgactggtc acattctccg ccataatcca gtcatcgaaa ccattaaaga agaggcaaat
     3061 ttcggtggga aagagcaagc ctttattcaa ttggacaatg gacgcagtgt ctttgtacga
     3121 ggtaaggtgg accggattga ccgtttgaaa gctgatggag cgataggagt agtagactac
     3181 aagtcgagtc taactcagtt ccagtttcct catttcttta atggactcaa ttcccaactg
     3241 ccaacctatc tagctgccct aaaaagagaa ggggagcaga actttttcgg tgctatgtac
     3301 ttggaaatgg ctgagcctgt ccaatctttg atggccgtta aaagtctggc aggtgcagta
     3361 gtagaagcca gcaagtcaat gaaataccaa ggcctctttt tagaaaaaga aagcagtcat
     3421 ttgggcgaat tttacaataa aaacaaggcc aatcagctga cggatgagga attccagctc
     3481 ctactggact acaatgccca tctttacaag aaagcggctg agaagatttt agaaggccag
     3541 ttcgctatca atccctatac cgaaaatggc agaagcattg ctccatgcgt ccagcaacat
     3601 caagccatca caggctttga agccaattac catttgggtc aagcccgttt cctagaaaag
     3661 ttggacttag ctgatggcaa gcgtctggtt ggagaaaaac tcaagcaagc ttggcttgaa
     3721 aaaataagag aggagttgaa tcgatgaagc ttattccctt tttaagtgag gaggagattc
     3781 aaaaactgca agaagcagaa gcaaattcga gcaaggaaca gaagaaaact gccgagcaaa
     3841 tcgaagctat ctacacttct ggccagaata tcctggtctc agcatcggct ggttctggaa
     3901 agacctttgt catggcagag cgcattctgg accaattggc gcgtggtgtc gaaatttctc
     3961 aactctttat ctcaaccttt accgtcaagg ctgcaactga acttaaagaa cgtttagaga
     4021 aaaaaatcag caagaaaatc caagaaacag atgatgtcga gctcaaacaa cacttgggtc
     4081 gccagttggc agacctaccc aacgctgcca tcggaaccat ggactctttc acacaaaaat
     4141 tccttggcaa acatggttat ctgcttgata ttgcacctaa tttccgtatt ttacaaaacc
     4201 aaagcgagca acttcttctc gaaaacgaag tctttcatga ggtctttgaa gcgcattacc
     4261 aaggtaaaca gaaagagacc tttagtcatt tgctgaaaaa ctttgctggg cgtggcaagg
     4321 acgaacgggg tctgcgccag caggtctata aaatctatga cttcctccaa tccaccagca
     4381 atcctcaaaa gtggctgagt gactctttcc tcaaaggctt tgagaaagct gattttacca
     4441 gtgaaaaaga aaaactgact gagcaaatca aacaagccct ttgggatttg gaaagctttt
     4501 tccgttacca tctggataac gatgccaagg agtttgcaaa ggctgcctat ttagaaaatg
     4561 ttcagttaat tctggatgaa attggctccc taaatcagga gtccgatagt caggcttatc
     4621 aggcagtgct tgcgcatgtt gtcgccatct ctaaggagaa aaacggtcga gctctgacta
     4681 atgccagccg taaggctgat ttgaagcccc tggctgatac ctacaacgaa gagagaaaga
     4741 cccagtttgc taaactagga caattatcag accagatagc gattctcgac tatcaagaac
     4801 gttatcatgg agacacttgg aaactagcta aaaccttcca atctttcatg agcgattttg
     4861 tagaggctta tcgtcagaga aaacgacagg aaaatgcctt tgaattcgct gatatcagcc
     4921 attacaccat tgagatttta gagaatttcc cacaagttcg tgagtcttat caggagcgct
     4981 tccatgaagt catggtcgat gagtatcagg ataccaacca tattcaagaa cggatgctgg
     5041 aattgttgtc taatggccac aatcgcttta tggtgggaga tatcaagcaa tccatctatc
     5101 gtttccgtca ggcagacccg cagattttca atgagaaatt ccaacgctat gcgcaaaatc
     5161 cccaagaagg caagctcatt ctcctcaagg aaaatttccg tagtagttca gaagtgctgt
     5221 cagcaaccaa tgatgtcttt gaacgtctca tggaccaaga ggtcggcgaa atcaactatg
     5281 ataacaagca ccagcttgtt tttgccaata ccaaactgac tcccaatcca gacaacaagg
     5341 cagaatttct cctctacgac aaggatgata caggtgagga agaagagagt caaacagaaa
     5401 cgaaactaac aggcgaaatg cgcttagtta tcaaggagat tctgaaactt catcaggaaa
     5461 aaggtgttgc ctttaaggaa attgcccttc taacctccag ccgcagtcgt aatgaccaga
     5521 ttctcctcgc cctgtctgag tacgggattc ctgtcaaaac tgacggagag caaaacaatt
     5581 atctccaatc cctagaagtg caagtcatgc tagacactct tcgtgtcatt cacaatcccc
     5641 tgcaagacta cgccttggtt gcccttatga agtctccaat gtttggtttt gatgaggatg
     5701 agctagcacg tttgtccctt cagaaagcag aggataaagt ccacgaaaat ctctatgaga
     5761 aactggtcaa tgcacaaaaa atggcaagta gtcaaaaagg cttgattcac acagctctag
     5821 ctgaaaaact aaagcaattc atggatatcc tagcttcttg gcgcttgtat gccaaaaccc
     5881 attctctcta tgacttgatt tggaagattt acaacgaccg tttttattat gactatgttg
     5941 gggctttgcc gaatggtcct gctaggcagg ccaatctcta tgccctagca ctgcgtgctg
     6001 atcaatttga aaagagcaat ttcaaaggtt tgtcgcgttt tattcgtatg attgaccaag
     6061 tcttagaagc ccagcacgat ttggcaagcg tggtcgtcgc accgccaaaa gatgcagtag
     6121 agctcatgac catccacaag agtaaagggc tggagtttcc ttacgtcttt atcctcaata
     6181 tggatcaaga tttcaacaag caagactcta tgtcagaagt cattctcagt cgtcagaatg
     6241 gtcttggtgt caaatatatt gccaagatgg agacaggggc agtagaagac cactatccta
     6301 aaaccatcaa actctccatt cctagtctga cctataggca gaacgaagag gaattacagc
     6361 tagcaagcta ttctgagcag atgcgtttgc tgtatgttgc tatgacgcgg gctgagaaaa
     6421 agctctatct tgtcggcaag ggttctcgtg aaaagctgga atccaaggaa tacccagcag
     6481 ccaaaaatgg gaaactaaat agcaatacta gactgcaagc acggaatttc caagattggc
     6541 tttgggctat cagtaaagtg tttactaagg acaagctcaa ctttagttat cgttttattg
     6601 gcgaagatca gttgaccaga gaagctatcg gagagttgga aaccaagagt cctctccaag
     6661 atagctccca agcagacaat cgtcagtcag ataccatcaa agaagctctg gaaatgctga
     6721 aggaggtgga agtttataat actcttcacc gcgcagctat tgaacttcct agtgttcaaa
     6781 ccccaagtca aatcaagaaa ttctacgaac cagttatgga tatggaaggt gtcgagattg
     6841 ctggtcaagg tcagtcagta ggcaagaaaa tcagcttcga tttgccagat ttttcaacca
     6901 aagaaaaggt aactggagct gagattggta gtgctactca cgaactcatg cagagaattg
     6961 acctcagcca gcaactaacc cttgctagcc taacagaaac actcaaacaa gttcaaacta
     7021 gccaagctgt cagagacaag atcaatcttg ataaaattct tgctttcttt gacacagcac
     7081 tcggtcagga aatttttgct aataccgacc atctctatcg cgagcaacct ttctccatgc
     7141 tcaaacgaga ccaaaagagt caggaagact ttgttgtccg tggtatcctt gatggctatc
     7201 tgctttacga aaacaaaatt gttttgttcg actacaagac agaccgctat gatgaaccaa
     7261 gtcaactcgt agaccgctat cgtggtcagt tagctctata cgaagaggct ttatcacgag
     7321 cctattcgat tgaaaatatt gaaaaatact tgattttact cggtaaagac gaggttcaag
     7381 ttgtaaaagt ataacttata ctcttcgaaa atctcttcaa accacgtcag cttcgccttg
     7441 ctgtatatat ggttactgac ttcgtcagtt ctatccacaa cctcaaaaca ctgttttgag
     7501 ctgactgcgg ctagctttct agtttgctct ttgattttta ttgagtatta gaaaggagac
     7561 ctcatgacac ttccagttag aaaatccctg cacgatgcag ttttacaggc ttcagaagcg
     7621 gatacttggg aacaagctac caaggaatgg aatgaagttt ccttgatttt taacggtatc
     7681 ggccgtagca attgtgtctg tggaaatgtc atcaaatacg cttatgaact ctttaacggt
     7741 gtcacaggcc aacgcctctt tccaattggt agcgactgtg ttcgtcattt tcatcgattg
     7801 tcccttgacc agcaattgga agaggaagaa aaactgctca gaaaggttga aaatctaacc
     7861 agaaaagctc agaaaaagga aaaaatcaag gtcaataaaa gtgactttga cgagcgactt
     7921 ctaaaatggc tctgggaaaa aggtgttttc aaacccaatc gtggcaatca atttgcgcct
     7981 gagagagact accagctttt cctagaagtc tttcagggag gaagttggac caaggcggaa
     8041 ccaaagaaaa aggctcggat ggaagaagtc cttgaaaagt gtatcaaacc ctttttactt
     8101 gggaaatcgg atgaccaact ctaccttgtc aagctaggca aggagaaaat tgactacgaa
     8161 caagaactcc gtatccaggc agagaaagaa cgcaagaaga gggataaaat cgccaagcaa
     8221 tatgcagata atctggttct agccatggga cccgcagagc gtgcttatca agattacttt
     8281 ggctttacag agaccctgac tcaagaagaa cgcaagtggg aaaaaattct ttttggtaaa
     8341 aatagagcag aacgggctat caaggccaaa caataccaaa aagagctgga aaaagaccaa
     8401 cgaattgcca gtcaggatcc aattgaaaga aaacagaagc agacttggct cctcaattcc
     8461 tattttcgtg agcttcctga agaaaaagct cgattttcta ggctcttatt agaatatcga
     8521 aaaagtggtg aagtaccctt ttcaactgaa tacctgtccg atcatctcat cgactttttc
     8581 tataaaatga aagcctttga atttgaaatc gcaccagaac aagtccgaga ttttctaaaa
     8641 aaaagcctgc aggaagacca tcgctcatca gcacaaggaa gctggataga aggaattctc
     8701 ctcaactgcc tcaaaccatt tttagaacga ttagtgatat gagtaacacc taccgtgtaa
     8761 atttcatggt aggtgttatt gtaccccaaa ataatataaa acaaagtatg aaagcgcatc
     8821 tcaattttaa gacttttttg taaattaaga gtataattaa ggtatactgc cttttctata
     8881 gataatagat aatacactaa tataataaag gtctttgtaa catatataaa ttttatttca
     8941 aggaggaata atggaaaagt attttggtga aaaacaagag cgtttttcat ttagaaaatt
     9001 atcagtagga cttgtatctg caacgatttc aagtttattt tttatgtctg tattagctag
     9061 ttcatctgtg gatgctcaag aaactgcggg agttcactat aaatatgtgg cagattcaga
     9121 gctatcatca gaagaaaaga agcagcttgt ctatgatatt ccgacatacg tggagaatga
     9181 tgatgaaact tattatcttg tttataagtt aaattctcaa aatcaactgg cggaattacc
     9241 aaatactgga agcaagaatg agaggcaagc cctagttgct ggtgctagct tagctgctct
     9301 gggaatttta atttttgctg tttccaagaa aaaggttaag aataaaacgg tattacattt
     9361 agtattggtt gcgggaatag gaaatggtgt cttagtttca gtccatgctt tagaaaatca
     9421 tcttttgcta aattacaata cggactatga attgacctct ggagaaaaat tacctcttcc
     9481 taaagagatt tcaggttaca cttatattgg atatatcaaa gagggaaaaa cgacttctga
     9541 ttttgaagta agtaatcaag aaaaatcagc agccactcct acaaaacaac aaaaggtgga
     9601 ttataatgtt acaccaaatt ttgtagacca tccatcaaca gtacaagcta ttcaggaaca
     9661 aacacctgtt tcttcaacta agccgacaga agttcaagta gttgaaaaac ctttctctac
     9721 tgaattaatc aatccaagaa aagaagagaa acaatcttca gattctcaag aacaattagc
     9781 cgaacataag aatctagaaa cgaagaaaga ggagaagatt tctccaaaag aaaagactgg
     9841 ggtaaataca ttaaatccac aggatgaagt tttatcaggt caattgaaca aacctgaact
     9901 cttatatcgt gaggaaacta tagagacaaa aatagatttt caagaagaaa ttcaagaaaa
     9961 tcctgattta gctgaaggaa ctgtaagagt aaaacaagaa ggtaaattag gtaagaaagt
    10021 tgaaatcgtc agaatattct ctgtaaacaa ggaagaagtt tcgcgagaaa ttgtttcaac
    10081 ttcaacgact gcgcctagtc caagaatagt cgaaaaaggt actaaaaaaa ctcaagttat
    10141 aaaggaacaa cctgagactg gtgtagaaca taaggacgta cagtctggag ctattgttga
    10201 acccgcaatt cagcctgagt tgcccgaagc tgtagtaagt gacaaaggcg aaccagaagt
    10261 tcaacctaca ttacccgaag cagttgtgac
//
LOCUS       DBERMR010000072        10238 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.072, whole genome shotgun sequence.
ACCESSION   DBERMR010000072 DBERMR010000000
VERSION     DBERMR010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10238
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.072"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..363
                     /locus_tag="ACL8A1_001962"
     CDS             <1..363
                     /locus_tag="ACL8A1_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0218 family bacteriocin-like peptide"
                     /protein_id="HGS1202669.1"
                     /translation="VLPNNYVVIDEEEMMYLDGGAIYIPRWAITGAITGAAYAALAAA
                     GGGGLQLVLASYGLRSALVAGIVKGLGVLGIHIGNAFANTVIRSIASAGIGAGADWIF
                     TNIIDGWDGRRDNQLRIG"
     gene            371..583
                     /locus_tag="ACL8A1_001963"
     CDS             371..583
                     /locus_tag="ACL8A1_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202670.1"
                     /translation="MMVVLISVIVAFLYHKITEYVNNRKIDIVIFLSLLISHFIWNYY
                     FLPIEILIAISGANLLLSFLKKLQIK"
     gene            957..1082
                     /locus_tag="ACL8A1_001964"
     CDS             957..1082
                     /locus_tag="ACL8A1_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202671.1"
                     /translation="MATITNALNIAATVAEVFSLGGAIAYGLDIVDGKFDGYLWA"
     gene            1063..1302
                     /locus_tag="ACL8A1_001965"
     CDS             1063..1302
                     /locus_tag="ACL8A1_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202672.1"
                     /translation="MGIYGRKTIAMKHFFAGIGEIHFLLFLLYPITSPIIGLVIGYNK
                     INLINSIIIALGMILALILVLSRVYRKYFYRDDDD"
     gene            1459..2517
                     /locus_tag="ACL8A1_001966"
     CDS             1459..2517
                     /locus_tag="ACL8A1_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202673.1"
                     /translation="MKDDQKYLLAGLYSLLVAIFYFPLIDSKGIFVSILMAVLLLYLI
                     YFIGTVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQ
                     EYCQGQPYGLLKKMLKRIRLSREISLLLATIIVYFFTHRILPLSVFTFIFSYILLFAQ
                     SYLGGNTAWIGNRRLIIDDEFEKILLSKSYIKEISSARYSEYLTCKYKNFTPIILLAI
                     FENLLDSYLLQNQSEVDLDIFYKVLPLLYKEKYTMGFNYFVSLNYLLYKVGFLGIIYD
                     NEALRDLSKQYLNKNISELQDGSFEGGIQDAVASKQIVVINEFIACLNSRCVPSQYDR
                     FFYKDRPYIFSRKSPIKG"
     gene            2832..3809
                     /locus_tag="ACL8A1_001967"
     CDS             2832..3809
                     /locus_tag="ACL8A1_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202674.1"
                     /translation="MKNKRYFFDTILIILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIFVIIIGARKYYRILFHQLMGFS
                     PGIAVFYKESQTTKNLFKFYYFLYFTTLISYYFFFTFVYDKPLLLPLISFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKKNCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYLSESVREQIDLLEVKRFKKINHPISLYGMLDVIKLNLYLRHYNEKNKYESMLNKIL
                     EVRPDFVLIEQNIDDSLNSSQPLSLSLAISEIQLLLEVYMGIKHVSIRR"
     gene            4186..4560
                     /locus_tag="ACL8A1_001968"
     CDS             4186..4560
                     /locus_tag="ACL8A1_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HGS1202675.1"
                     /translation="MELVLPNNYVVLEQEEMMYLDGGFSIPRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGN
                     AITTIWANNDVNRRDPNLNALW"
     gene            4567..4749
                     /locus_tag="ACL8A1_001969"
     CDS             4567..4749
                     /locus_tag="ACL8A1_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202676.1"
                     /translation="MGIKMEKIFVIIFFVCLFISSITFLAYDFVSEEIKKLIIWINVV
                     FLILIIAMIIYPKLRK"
     gene            4768..5670
                     /locus_tag="ACL8A1_001970"
     CDS             4768..5670
                     /locus_tag="ACL8A1_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202677.1"
                     /translation="MIRKLIIFLLMLPLFVLWLELHLLVSNLQLNLEIPFDFVVSTSL
                     TFFVLILSKIVLDILYALKDLYKKEALITIFPFIFIGRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFAIIMLTTIFFWTIMDINHLVFNKTEFLFNQN
                     KWQKEDSFESDLTKTLKDKIQKSELSYSDLMSLLLYDAMNQSTFLTDSELFEDILKKI
                     EDSHNTLLCTGLVELLLYEISISKNHNWSEKIDKIRIELIKINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
     gene            5726..6079
                     /locus_tag="ACL8A1_001971"
     CDS             5726..6079
                     /locus_tag="ACL8A1_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HGS1202678.1"
                     /translation="MELVLPNNYVVIDEEVMMYLDGGAYLSKRACKGICAALAMSSGT
                     FIALAGAAVLTKKLINYIKFGGLGGWLIGAAAGVLATAAEKIAYCIGYGVLNRGCDIN
                     GNPYPWDGFISATVR"
     gene            6076..6804
                     /locus_tag="ACL8A1_001972"
     CDS             6076..6804
                     /locus_tag="ACL8A1_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202679.1"
                     /translation="MSNVDKIRKIHIIVCWVYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLISFPILINKFFPESFLTYTVLISVFITELIIFHL
                     IGKDFDTKLTNEYKKISQFRSKVSQSPWIKYLEISSFILTIFPSILYGTVDNHVLTLI
                     FLIKICVDTTIKFLFIRLFDTSTLMKRRIFFLFALDVIAYLFLGYLLVIQKAGYLFSI
                     LLLFSNFSVPFIKEKEYELFKNSK"
     gene            7283..7480
                     /locus_tag="ACL8A1_001973"
     CDS             7283..7480
                     /locus_tag="ACL8A1_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202680.1"
                     /translation="MKHFFAGIGEIKLVGYLLYICVGLAPLFHIYVIGSELSFVKIVL
                     SILGVIFVSMLTIARIYRTFL"
     gene            7936..8175
                     /locus_tag="ACL8A1_001974"
     CDS             7936..8175
                     /locus_tag="ACL8A1_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202681.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     DLHKITRDYASQTKPAIQDGVFILEDFRNILCTMM"
     gene            8728..>10238
                     /locus_tag="ACL8A1_001975"
     CDS             8728..>10238
                     /locus_tag="ACL8A1_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HGS1202682.1"
                     /translation="MNKKKMILTSLASVAILGAGFVASSPTVVRAEDAPVANQSKAEK
                     DYDAAMKKSEAAKKHYEAAKKKAEDAQKKYDEDQKKTEEKAAKELKASKEIAEATSQV
                     QNGYVEYLKVQSATNINIKERQKKLAEIDEKINNAQQTLKKKQEELGKVRAEVIPEPA
                     ELAKTRKKAEEAKAEEKVAKKKYDEATQEVALAKKKVETEEAELDKKVAELQNKVTDL
                     EKEIADAEKTVADLEKEVAKLEKDVEGFKESDGEYAEFYLEAAEKDLATKKAKLAEAK
                     IKAATKKAELEPELEKAEAELENLLSTLDPEGKTQDELDKEAEEAELDKKADELQNKV
                     AELEEELSKLEDNLKDAEINNVEDYIKEGLEEAIATKQAELEKTQKELDAALNELGPD
                     GDEEETPAPAPQPEKPAPAPKPEQPAPAPKPEKSADQQAEEDYARRSEEEYNRLTQQQ
                     PPKAEKPAPAPKPEQPAPAPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYL"
BASE COUNT         3539 a         1357 c         1881 g         3461 t
ORIGIN      
        1 gtattaccaa ataattatgt tgtgattgat gaagaagaga tgatgtacct tgatgggggg
       61 gctatatata tacccaggtg ggcaattaca ggagccatta ctggtgcagc atatgcagca
      121 ttagcagcag caggaggtgg aggccttcaa ctagttcttg catcttatgg attacgctcc
      181 gcactggtag ctgggattgt taaaggttta ggagtattag gaattcatat tggaaatgct
      241 tttgcaaata ctgttattag aagtattgca tctgctggaa ttggtgctgg agctgattgg
      301 atttttacca atattattga tggctgggat gggcgacgtg ataatcaatt gagaatagga
      361 taatagtatt atgatggttg ttttaatttc agttattgtt gcattcctgt accataaaat
      421 tacagagtat gtcaacaaca ggaaaattga tattgttatt tttctatcat tgctgatttc
      481 tcattttatt tggaattact attttttacc aattgaaata ttgatagcaa tcagtggagc
      541 aaatttatta ttaagttttt taaaaaaatt acagattaaa taatattatt ccatatgaca
      601 tatcaactag gtcattagat tttattgaag atataaatta aaaacgaggt tgggaaaaaa
      661 ctcaatttga aagtgaaatt gagtaaatct ttctatctat ataacgtata tcatcaatat
      721 ttttgaacac ctgataatat gcatttttct tattttaaag attttttgcc tttcttcatt
      781 tcttgaatga attggagaga tgactttatt taggtgttta acattactat agataggatt
      841 tagcagtact aatatgaact catattatag gtgtagactt aaattttgga agcaagctaa
      901 aggatatctt atctagataa ctaagtgcta taacaagata tattggtgga gttagtatgg
      961 caactattac taatgctctt aatattgcgg ctactgttgc tgaagtcttc tcattaggag
     1021 gagccattgc atatggttta gatatcgtag acggtaaatt tgatgggtat ttatgggcgt
     1081 aaaactatag ctatgaaaca tttttttgca ggaattggtg aaattcattt tttgttattt
     1141 ttattatatc caatcactag tccaattatt ggactagtga ttggatataa taaaataaat
     1201 ctaataaatt ctattattat agctcttggt atgatattgg cattaatttt ggtattatca
     1261 agagtgtata gaaaatattt ttatagagac gatgatgact aaagttttta tcaataattt
     1321 gggctccttg tcaactgtag tgggttgaag aaaagctaac gtctggggaa gggtttaaag
     1381 ctttgcttta tcaaatattg acttacagag ttgctctatt gtattgatgt attcttagta
     1441 aataatatgg tgttttgaat gaaagatgat cagaaatatt tattagcggg actttattcg
     1501 cttctagttg caatatttta ttttccattg attgattcca aggggatttt tgtaagtata
     1561 ttgatggcgg ttttactttt atatttaata tacttcattg gaacagtcat tcatattgtg
     1621 ataattaagt ttataagaaa aaaatctttt aagtatttgg ttctatatcc atttacttat
     1681 gatgggagct ggagatttca acctataaac ttactctact ttccagaaat ggtgagagat
     1741 gtcattccga ttaatttagt tcaagaatat tgccaaggac aaccttacgg actgttaaaa
     1801 aagatgctga aaagaattcg attgtcacga gagatatctt tactcctggc taccattatc
     1861 gtctattttt ttacgcatag aattctacct ctttcagtat ttacgttcat attttcttac
     1921 atattgctat ttgcacaatc ttatttgggt ggtaataccg cttggattgg aaatagaaga
     1981 ttaataatag atgatgaatt tgaaaaaatc ttgctttcaa aaagttatat aaaagaaata
     2041 tcatctgcac gatactcaga atacttgaca tgtaaataca aaaactttac accaatcata
     2101 ttgttagcta tttttgaaaa tttgcttgac tcctacttgc ttcagaatca gagtgaggtt
     2161 gacttagata ttttttataa agttttacct ttgctttata aagaaaaata tactatggga
     2221 ttcaattact ttgtaagttt aaattattta ctatataagg ttggattttt gggaatcatt
     2281 tacgataatg aagcattaag agatttgtca aaacaatatt taaacaaaaa tatttcggaa
     2341 ttacaagatg gttctttcga gggcggtata caagatgctg ttgcctctaa acaaatagta
     2401 gttataaatg aatttatagc ttgtttaaat tccagatgcg tgccatccca atatgataga
     2461 tttttttata aagatagacc ttatattttt tccagaaaga gtcctattaa ggggtgaaaa
     2521 tttgttacat ttggattaac aagtgtccta gggattagaa ataactctag tatagctaga
     2581 tatttttgag taactagaaa tatcattagt ctacccatat gattgccaga aaagatgact
     2641 gatgaatctt atttatactg ttgcagtagg cgttgtctag gataaataag ctatcttgat
     2701 gggggagatt ggaataattt tagtagaaac attaaaggat tatggacaag tagcgtagga
     2761 ttaggtgatg caatgtgcta agttggcttg gtgttagcag tagtattatt taacaatgga
     2821 aagtagacta aatgaaaaat aagcgttatt tttttgacac aattttaata attttactgc
     2881 ttataagcac gatattttgt gtcagcccag tatttataaa attggacatc ttaggaactc
     2941 cctctcatgc catcttaacc tttgtattgg cgataccgtt attctatatt ttgtctcaat
     3001 gcttacatac tttgctatta ctagtttcct caatcttttg taaattaaga ccaatttatt
     3061 tttattttat atttgtgatc attataggag ctaggaaata ctatcgaatt ctctttcacc
     3121 aactaatggg atttagtcca ggaatagcag tgttttataa ggagagtcaa acaactaaga
     3181 atctgtttaa attttactac ttcttgtact ttacaacact aatttcatac tattttttct
     3241 ttacttttgt gtatgacaaa ccgttgcttt tgcctcttat ttcattttct attatcatag
     3301 cattagtaca aaaactttat agaattgaga atcaacaatt atttctgtta aaatcaaagg
     3361 tactaacaat actggaatct aagaagaatt gtgagtttaa tcttcaggat tatcatgaaa
     3421 tttggaaatt gcaatcaaaa tctgagttgc catgcgtggc tttatcttat atcagtttaa
     3481 tcaagccata tctttcagag agtgtgcgtg aacaaattga tttattagaa gttaagcgat
     3541 ttaaaaaaat aaatcatcca atatcacttt atggtatgtt ggatgttata aaattaaatc
     3601 tatatttacg gcattataat gaaaaaaata aatatgaatc aatgttaaat aaaattttag
     3661 aggtaagacc agactttgtt ttaatcgagc agaatattga tgatagttta aatagctcgc
     3721 aaccattatc tttatcttta gccatttcag aaatccaact tttgcttgaa gtgtatatgg
     3781 ggatcaaaca tgtttcaatt aggagataaa accagaataa tatttgccat tataggggca
     3841 agttgcatat tagtcagtat gtatcttata cctctcatta acacggataa taaattttgg
     3901 gaaatacttt tatttatatt aggtgtattg ctaattggaa ttgcgttact aagtcaaaaa
     3961 aaataaattt tgaaatagta ttagtgagtt cgcaatcatt tattgctagt ggtttagaac
     4021 tgttaagttt caaacaataa ttgattttaa accttaggta acctataaaa acaaataagg
     4081 tagagaaagg atatcttatc taggtagcta agtgataaaa acacagtcgt ctatcacttg
     4141 ttaggaaact atcctaacta tgcttaataa aaaaggagaa aataaatgga actcgtatta
     4201 ccaaataatt atgttgttct tgagcaagaa gagatgatgt atcttgatgg gggattttct
     4261 attccgagat ggcctgttgc aacagccatt aatatagctt ttaatggtgt tttaggtgga
     4321 ggagcaatca gtctagttag aaattatatt cgtaattatg gtttgcggcg agttacaagc
     4381 gcaattgctg gagcagctgc aagatatgtt ggggtacgag ttgcaaatag agtggcagga
     4441 tttgcactgt ctgctattaa tggatttgca gcttggatgt caattggcaa tgctattaca
     4501 acaatctggg ccaataatga tgtaaatagg agagacccaa atttaaacgc cttgtggtaa
     4561 ctagatatgg gtattaaaat ggaaaaaatt tttgttatta ttttttttgt atgtctattt
     4621 atatcatcca taactttttt agcctatgat tttgttagcg aagaaataaa aaagttgatt
     4681 atttggatta atgtcgtttt tttgatttta atcatagcaa tgataattta tccaaaatta
     4741 agaaaatgat aaggacactg taaaattatg atacgtaaac taatcatttt tttattaatg
     4801 ctccctcttt ttgtcttgtg gctagagttg catttgttag tgagtaactt gcaattgaat
     4861 ctagaaattc cctttgattt tgtggttagt acgagtctta ctttttttgt tttgatatta
     4921 tcaaaaatag ttttagacat actatacgca ttgaaagatt tgtacaagaa agaagctctt
     4981 attacgattt ttccttttat ttttataggc aggaagaaag taaatgtgag gttctcgccc
     5041 tatttttcat tccatcgtaa aagtttgtcg cctgatgatt taagatctag gattatatgg
     5101 agttttattc ttgaaattgc tattatccta gtatttattt taaaaattcc ttttgcaatc
     5161 atcatgctta ctacaatatt tttctggact ataatggata tcaatcatct cgtttttaat
     5221 aagacggaat ttctttttaa tcaaaacaaa tggcaaaaag aagattcgtt tgaaagtgat
     5281 ttgacgaaaa cgttaaagga taagatacaa aaatcagaat taagctattc tgacttaatg
     5341 tcccttcttc tctatgatgc gatgaaccaa tccacctttt taacggatag tgaacttttt
     5401 gaggatattt tgaaaaaaat cgaagattct cataacaccc ttctgtgtac aggtcttgtc
     5461 gagttactgc tgtatgaaat ttctattagt aagaatcata attggtcaga aaagattgat
     5521 aaaattagga tagagcttat caagattaat caattagatt ttttctatta tacaagttgg
     5581 ttaaggcaaa attttgattt ctgtatgaat agagagtatc ataaaatgaa atctcggaaa
     5641 ttattactga gtaataaaaa gatagtttaa gttcgtccta tttgtaaaac aaatgaaaca
     5701 atttcaacta aggaggagaa aacaaatgga acttgtatta ccaaataatt atgttgtgat
     5761 tgatgaagaa gtaatgatgt atcttgatgg gggagcttat ttaagcaagc gtgcttgtaa
     5821 aggaatttgc gcagctttag ctatgagttc aggaactttt atagcattag ctggagctgc
     5881 agttttaacc aaaaaactaa taaactatat taagtttgga ggtcttggag gctggcttat
     5941 tggtgcagca gcaggtgtat tggctacagc agcagaaaaa atagcttact gtattggata
     6001 tggtgttctt aatagaggtt gtgatattaa cgggaaccct tatccttggg atggattcat
     6061 atctgcaaca gtaagatgag taatgtagat aagattcgaa aaatacacat catcgtgtgt
     6121 tgggtgtata tatttttatc attcagggcg attataaatg atacggagta ttttttgctt
     6181 atttttttag cttttatcta ctcaatagta tcactacctc tatattctgt gaaaaataaa
     6241 atagtatcta tctgtctagc cataaattcg attctgttaa tcagtttccc cattttaatc
     6301 aataagtttt tcccagaaag ttttttgact tataccgtat taataagtgt ttttattaca
     6361 gagttgataa tttttcattt aattgggaaa gattttgata ctaaattgac taacgaatat
     6421 aagaaaatta gtcagtttag aagcaaggtg tctcaatctc cttggataaa atatttagag
     6481 atttctagtt ttatattaac tatatttcca tctattcttt atggtacagt tgataatcat
     6541 gtactgactc ttatcttttt gataaaaatt tgtgtagata ctacgataaa atttttattc
     6601 atcagattat ttgacacaag tactttaatg aagaggagaa tattttttct tttcgcattg
     6661 gatgttatag cttacttatt tttaggatat cttttagtga ttcaaaaagc aggatatctg
     6721 ttttcgattt tacttctttt ctctaatttt tcagttccat ttatcaaaga aaaggaatac
     6781 gaattattta aaaatagcaa gtaatagaaa cataggttaa atcttagaac ggcttaaaat
     6841 cagatatgat aaagggaaat agtcttatct aggtaactaa gtgctacaaa cacaatcgag
     6901 gatcacttgt taggattaca ttttaatcat gcttaataag aatggagaat tcctatgggg
     6961 attgtattac tctgaagctt cgaatacctt agttctcgtg attttgagct attctaggtt
     7021 tagaatcctt cagaatgtaa aattttaagc agtcaaagac ttagaatagc ctgaaaacat
     7081 ttattttata acaattctag gcttagaatt gctcatatat atgtatttga gctgctcgat
     7141 agttaggaca gctctattct gagaaatctg agctatcaat gcttcagaat agtatttcgc
     7201 caatggtttt tattgaagag gtgattgtgt tatgatagtc taaaataaaa ttgatttaat
     7261 agagtaaatg gagaatcgtt acatgaaaca tttttttgca ggaattggtg agataaaact
     7321 tgttggctac ttattgtata tatgtgtagg actagcccct ctttttcata tatatgtcat
     7381 aggttcggaa ctgagttttg tgaaaatagt gttatctatt ttgggagtta tatttgtaag
     7441 tatgttaact attgctcgaa tttatcggac ttttctataa agaatagttt ggacttgaac
     7501 tgtatctaaa aacagaggtt ggtagattat cagcctcttt cttgtcgttg aaaagagtga
     7561 gatatattga cttttgaaaa aaatgtcata attgagttgg gataaaccta aaagtaatat
     7621 agtagattga atctggaata gtccgttatg actgttaaaa tatttctaga aattaatttg
     7681 actttcctaa tcgatttgtt cacatcttgt ttcaatctac tatactttct cgttaaaccg
     7741 ttcggtaatt catggtgaca tggacacata gtagtaagca cactatgtgg cttggcagat
     7801 ctaaaaactg ttcccttgcg ataagcctaa taagcacaac atagggagtt agagaagccg
     7861 actctaatca accactttgg gcagtagtgg taactgcccc gtgcttttta ttttgagaaa
     7921 atatggagtt tgtcgttgaa attacttgat tgtattttgg attatcaaga aagattcaat
     7981 ggaaaaacat gtcaagtatc aacgaattat aagtatttag agattttcaa agtgaatttt
     8041 tgcttgactg atttacatca tttatttgac ttacacaaaa tcacacgaga ttatgctagt
     8101 caaacaaaac ctgctattca agatggtgtt tttattttag aagattttag aaatatccta
     8161 tgtacaatga tgtaatcgaa aggatatctt tatgtgagtt tatcggcgat atcttctata
     8221 tctaagctag caagttgttg catcgtagct aaggatttat ctaaaaatac tatgaaattg
     8281 gacgtcatat tttttgagga taaaaataaa agatccgcag ttttaggttt acgaagagac
     8341 aaaagcggag tatttaaacc agttactcta cattttacaa gcgctaagaa atatgctaaa
     8401 gttcgtaaaa cagatgtgaa agaaatgaaa tggctataaa gattgtccgc aggcttaagc
     8461 ttgcgttttt tgttgtttaa cgaaattaaa aaacaagctc tctcatcgga aatgttttta
     8521 taggaatgaa ggaagatgat gctatgttta aaaagagaat cgaaaagggc aaaagtagta
     8581 tctttatcta ttttttggag tagaagctta tgatatagaa atttgtaata aaaatgtaat
     8641 ataaaacatt tgacaaatat ttacgggggg agtatactta atataattat agtctaaaaa
     8701 tgactatcag aaaagaggta aatttagatg aataagaaaa aaatgatttt aacaagtcta
     8761 gccagcgtcg ctatcttagg ggctggtttt gttgcgtctt cgcctactgt tgtaagagca
     8821 gaagacgctc ctgtagctaa ccagtctaaa gctgagaaag actatgatgc agcaatgaaa
     8881 aaatctgaag ctgctaagaa acattatgaa gcggctaaaa agaaagcaga agacgctcag
     8941 aagaaatatg atgaggatca gaagaaaact gaggaaaaag ctgctaagga attaaaagca
     9001 tcgaaggaaa tagcagaagc cacgtctcaa gtccaaaatg ggtatgtaga atatcttaaa
     9061 gtccaatctg ctactaatat aaatataaaa gaacgacaaa aaaaattagc agaaattgat
     9121 gaaaaaataa ataatgctca gcaaacttta aagaagaagc aggaagagct tggtaaagtt
     9181 agggcagaag taattcctga accagctgag ttagctaaga ctcggaaaaa agcagaagag
     9241 gctaaagcag aagaaaaagt agctaagaaa aaatatgatg aggcaactca agaggtagca
     9301 ctagcgaaga agaaagtaga aacagaagaa gctgagttgg ataaaaaagt tgctgaactt
     9361 caaaataaag ttactgattt agaaaaagaa atagctgatg ctgaaaaaac ggtagcagat
     9421 ttagaaaaag aagttgctaa acttgaaaaa gatgtagaag gtttcaaaga atcagatggt
     9481 gaatatgccg aattctacct tgaagctgct gaaaaagacc tagctactaa aaaagctaaa
     9541 ttagcagaag ctaaaattaa agctgctact aaaaaagctg aattagaacc tgaattagaa
     9601 aaagcagaag ctgaacttga aaacctatta tctacactag accctgaagg taaaactcaa
     9661 gatgaattag ataaagaagc agaagaagct gagttggata aaaaagctga tgaacttcaa
     9721 aataaagttg ctgaattaga agaagaactt tcaaaacttg aagataatct taaagatgct
     9781 gaaataaaca acgttgaaga ctacattaaa gaaggtttag aagaagctat cgcgactaaa
     9841 caagctgagt tagaaaaaac tcaaaaagag ttagatgcag ctcttaatga gttaggccct
     9901 gatggagatg aagaagagac tccagcgccg gctcctcaac cagaaaaacc agctcccgca
     9961 ccaaaaccag agcaaccagc tcccgcacca aaaccagaga agtcagcaga tcaacaagct
    10021 gaagaagact atgctcgtag atcagaagaa gaatataacc gcttgactca acagcaaccg
    10081 ccaaaagcag aaaaaccagc tcccgcacca aaaccagagc aaccagctcc cgcaccaaaa
    10141 acaggctgga aacaagaaaa cggtatgtgg tacttctaca atactgatgg ttcaatggca
    10201 acaggttggc tccaaaacaa cggttcatgg tactacct
//
LOCUS       DBERMR010000073        10093 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.073, whole genome shotgun sequence.
ACCESSION   DBERMR010000073 DBERMR010000000
VERSION     DBERMR010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10093
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.073"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..956)
                     /locus_tag="ACL8A1_001976"
     CDS             complement(<1..956)
                     /locus_tag="ACL8A1_001976"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGS1202683.1"
                     /translation="MKSDSILIMMATYNGEEFLREQLDSILCQTISTWELLIRDDNSN
                     DQTRAIIKEYEEKDRRIKLIENISEYHGAYYNFWGLLNDVRQNENPFDFYMFADQDDI
                     WDVDKLERLISYYYRKVKTEEPVLIYADMRIIDANGKVIANSMDQLMGIRYTNPISTF
                     MAHKVYGCNTLFNHELFEILPLLPCYAPELAFLSHDNFTTKIAALKGHVYFYDEPTMS
                     YRRYGHNVTSKHEYNFTLKRILKRISKIDELAKDHALTYKQTLVATNLLHQQTSIDTE
                     LLFLDKVEKIIKKGGLNAVKMVWKEKIDWGNRVKTISRSLILA"
     gene            complement(946..2001)
                     /locus_tag="ACL8A1_001977"
     CDS             complement(946..2001)
                     /locus_tag="ACL8A1_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HGS1202684.1"
                     /translation="MTLLLNKIYRQDVESSLETIVNINDLNGKSILITGASGLIGSSV
                     IDQLLLLNEIRNFNIKIFCCGRKFESLEKRFGRQTEYLRFVFYDATKAPHFDFAVDYI
                     IHAASPASPELYVNQPVETMASNFLGMYNLLTYAKNAKVSNLVYISSSEVYGIGTSNN
                     PLEESYIGTVDHLSVRSSYASSKRATETLCASFASEYGIKIMIVRPGHIYGPSAKKSD
                     NRVSSFFMIEAIAGRDIIMKSTGSQLRSYCYSLDCASAILSVLLSGESGQAYNVSNPN
                     SIITIKQMAEIISRVGNVKLTMDIPSDQDIKQTNPMQNAGLSSDKLEELGWQGHFTAE
                     EGFQHTFNILKELSSEK"
     gene            complement(2004..2723)
                     /locus_tag="ACL8A1_001978"
     CDS             complement(2004..2723)
                     /locus_tag="ACL8A1_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HGS1202685.1"
                     /translation="MKVAILTASGIGSRIGQDIPKQFIHVENKPVIIYTLEKFQNHPE
                     IDEICIVILKGWEQMVKAYAEQFGITKLKMITLGGKSGQESIYNGLREVKKAHPNEDV
                     TVLIHDGNRPLVSNDIISNALATYQQFGNAVAAIPTTEVVFVLENPQSTSSTEALNRD
                     LLRRTQTPHVYHLDNILSLHEKALENGITDVAASCQLMQLFGEKSYFSLGTEKNLKIT
                     TVEDLDIFKALLSSTRDKWIK"
     gene            complement(2724..3620)
                     /locus_tag="ACL8A1_001979"
     CDS             complement(2724..3620)
                     /locus_tag="ACL8A1_001979"
                     /inference="COORDINATES: protein motif:HMM:NF016852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HGS1202686.1"
                     /translation="MAVKLKYDKSDITFLDDDGLKKYQSELLKMISDVFTFFDENGIA
                     YSLSGGSILGAIRHKGFIPWDDDVDINIPRESYDKLFSLFESDNSLSRKYYLQSAKSH
                     PELGLHVSQIRKKGTVARRKYDHSAEECGISIDLYIVENVYNNPVKRFFQGYTSMFLT
                     FALASVRETKNHALMKEMFRLEGRKLNYSTGKLMVGCFFGIIPIEKWLNWLDKCNSSC
                     KDSHTKYVSIPTGRKHFRRETYLRENMNIYKKVPFETLMVNVPVWSEEYLEMFYGKDY
                     MVVPPVDKREQHLFLELSYGEE"
     gene            complement(3626..4798)
                     /gene="cps2T"
                     /locus_tag="ACL8A1_001980"
                     /gene_synonym="wchF"
     CDS             complement(3626..4798)
                     /gene="cps2T"
                     /locus_tag="ACL8A1_001980"
                     /gene_synonym="wchF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta 1-4 rhamnosyltransferase Cps2T"
                     /protein_id="HGS1202687.1"
                     /translation="MKKSVYIIGSKGIPAKYGGFETFVEKLTAFQQDKAIQYYVACMR
                     ENSAKSGTTEDVFEHNGAICYNVDVPNIGPARAIAYDIAAINRAIEIAKENKDEDPIF
                     YILACRIGPFIHGIKKKIQAIGGTLLVNPDGHEWLRAKWSAPVRRYWKISEGLMVKHA
                     ALLVCDSKNIEKYIQEDYIQYQPKTTYIAYGTDTTRSTLKSSDEKVRSWFKEKNVSEN
                     EYYLVVGRFVPENNYESMIRGFLASNSKKDFVLITNVEQNKFYNQLLAKTGFDKDPRV
                     KFVGTVYEQELLKYIRENAFAYFHGHEVGGTNPSLLEALASTKLNLLLDVGFNREVGE
                     QSAIYWKKDELSQVIEKVEQFDAKMIDELDRQSNQRIADYFTWEKIVTDYEKVFKG"
     gene            complement(4829..6196)
                     /locus_tag="ACL8A1_001981"
     CDS             complement(4829..6196)
                     /locus_tag="ACL8A1_001981"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HGS1202688.1"
                     /translation="MNGKIVKSSLAIIQSFLVILLTYLLSAVREAEIVSTTAIALYIL
                     HYFVFYISDYGQDFFKRGYLIELVQTLKYILFFALAISISNFFLEDRFSISRRGMIYF
                     LTLHALLVYVLNLFIKWYWKRAYPNFKGSKKILLLTATSRVEKVLDRLIESNEVVGEL
                     VAVSVLDKPDFQHDCLKVVAEGEIVNFATHEVVDEVFINLPSEKYNIGELVSQFETMG
                     IDVTVNLNAFDRSLARNKQIREMAGLNVVTFSTTFYKTSHVIAKRIIDIVGALVGLIL
                     CGLVSIVLVPLIRKDGGSAIFAQTRIGKNGRQFTFYKFRSMCVDAEAKKRELMEQNTM
                     QGGMFKVDDDPRITKIGRFIRKTSLDELPQFYNVLKGDMSLVGTRPPTVDEYEHYTPE
                     QKRRLSFKPGITGLWQVSGRSEIKNFDEVVKLDVAYIDGWTIWKDIEILLKTVKVVFM
                     RDGAK"
     gene            complement(6211..6900)
                     /locus_tag="ACL8A1_001982"
     CDS             complement(6211..6900)
                     /locus_tag="ACL8A1_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HGS1202689.1"
                     /translation="MPTLEISQAKLDSVKKAEEYYNALCTNLQLSGDGLKVFSITSVK
                     IGEGKSTTSANIAWAFARAGYKTLLIDGDIRNSVMLGVFKARNKITGLTEFLSGTTDL
                     SQGLCDTNIENLFVIQAGSVSPNPTALLQSKNFTTMLETLRKYFDYIIVDTAPVGVVI
                     DAAIITRNCDASILVTEAGEINRRDIQKAKEQLEHTGKPFLGVVLNKFDTSVDKYGSY
                     GNYGDYGKNKK"
     gene            complement(6910..7602)
                     /locus_tag="ACL8A1_001983"
     CDS             complement(6910..7602)
                     /locus_tag="ACL8A1_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HGS1202690.1"
                     /translation="MKEQNTIEIDVFQLLKTLWKRKLMILIVALVTGAGAFAYSTFIV
                     KPEYTSTTRIYVVNRNQGDKPGLTNQDLQAGSYLVKDYREIILSQDALEKVATNLKLD
                     MPAKTLASKVQVTVPADTRIVSISVKDKQPEEASRIANSLREVAAEKIVAVTRVSDVT
                     TLEEARPATTPSSPNVRRNSLFGFLGGAVVTVIAVLLIELLDTRVKRPEDVEDVLKIP
                     LLGLVPDFDKIK"
     gene            complement(7611..8342)
                     /gene="cps4B"
                     /locus_tag="ACL8A1_001984"
     CDS             complement(7611..8342)
                     /gene="cps4B"
                     /locus_tag="ACL8A1_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4B"
                     /protein_id="HGS1202691.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLTESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVASDLVIAYGAEIYYTPDVLDKLEKKRIPTLNDS
                     RYALIEFSMNTPYRDIHSALSKILMLGITPVIAHIERYDALENNEKRVRELIDRGCYT
                     QVNSSHVLKPKLFGERYKFMKKRAQYFLEQDLVHIIASDMHNLDGRPPHMAEAYDLVT
                     QKYGEAKAQELFIDNPRKIVMDQLI"
     gene            complement(8344..9789)
                     /gene="cps4A"
                     /locus_tag="ACL8A1_001985"
     CDS             complement(8344..9789)
                     /gene="cps4A"
                     /locus_tag="ACL8A1_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4A"
                     /protein_id="HGS1202692.1"
                     /translation="MSRRFKKSRSQKVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLLFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSDIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKLIDLLGGIDVYNDQ
                     EFTAHTNGKYYPAGNVHLDSEQALGFVRERYSLADGDRDRGRNQQKVIVAILQKLTST
                     EALKNYSTIINSLQDSIQTNMPLETMINLVNAQLESGGNYKVNSQDLKGTGRTDLPSY
                     AMPDSNLYVMEIDDSSLAVVKAAIQDVMEGR"
     gene            10023..>10093
                     /locus_tag="ACL8A1_001986"
     CDS             10023..>10093
                     /locus_tag="ACL8A1_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001839528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 transposase-related protein"
                     /protein_id="HGS1202693.1"
                     /translation="MAYSIDFRKKVLSYCERTGSITEA"
BASE COUNT         3087 a         2125 c         1595 g         3286 t
ORIGIN      
        1 gctaaaatca gagacctgct tatagtctta acacgatttc cccaatcaat tttttctttc
       61 caaaccattt ttacagcatt taatccacct tttttgatta ttttttctac cttatctaaa
      121 aataggagtt cagtgtcaat acttgtttgt tgatgtaaca aatttgtagc gactaaggtc
      181 tgcttgtaag tcaaggcatg atctttagct aattcatcaa ttttcgagat acgttttaag
      241 attcgcttca atgtaaagtt atactcatgt ttacttgtaa cattatgccc atatcgtcta
      301 taactcatag taggctcatc ataaaaatac acatgccctt tcaatgcagc aatttttgtt
      361 gtaaagttat catgtgatag gaaggctaat tctggagcat aacatggaag gaggggtaag
      421 atttcaaata attcatgatt aaataacgta ttacatccat aaaccttatg agccataaaa
      481 gttgagatag gattggtata ccgaattccc atcaattgat ccatactatt agctatcact
      541 ttaccgttag catctataat ccgcatatca gcgtaaatca ataccggttc ttctgtttta
      601 acttttctat agtaataact aattaaacgt tctaatttgt ccacatccca gatatcatcc
      661 tgatcagcaa acatatagaa atcaaatgga ttttcgtttt gtctaacatc gtttaataac
      721 ccccaaaaat tataataggc tccatgatat tccgatatgt tttcaatcaa tttaatccgt
      781 ctatcttttt cttcgtattc tttaataatt gctcttgttt gatcattgga attatcatca
      841 cgaatcagta gttcccaagt tgagatggtt tgacaaagga tactatccaa ctgttcacga
      901 agaaattctt ctccgttgta ggtagccatc atgattaaaa tgctatcact tttcactgga
      961 taactccttt aaaatattaa atgtatgttg aaaaccttcc tcagctgtaa aatgtccttg
     1021 ccatcctaat tcttctagct tatcactgct tagaccggcg ttctgcatag gattagtctg
     1081 cttaatatct tggtcactag gaatatccat ggtgagtttc acattgccaa ctcgagaaat
     1141 aatttcagcc atctgtttta tagtaataat tgaatttgga ttggaaacat tataagcttg
     1201 ccctgattca cccgaaagga gaacacttaa aatagcacta gcacaatcca aactataaca
     1261 atacgatctc aactgactcc ctgtactttt cataatgata tcacgaccag caatcgcttc
     1321 tatcataaaa aatgatgata cacgattatc acttttcttg gcactagggc catatatgtg
     1381 tcctggtctt actatcatga ttttgatccc gtactcggaa gcaaaactag cacaaagtgt
     1441 ttcagtagct ctcttagaac tagcatacga agaacgtacg ctaagatgat caacagttcc
     1501 aatataactc tcctccaaag gattattact ggtgccaatt ccataaactt cactagatga
     1561 gatataaact agatttgaaa ctttagcatt ttttgcgtat gtcagtagat tatacatgcc
     1621 cagaaaatta ctagccattg tttctacagg ctgattaaca tataattcag gactagcagg
     1681 actagcagca tgaataatgt agtccactgc aaaatcaaag tgaggtgctt tagtcgcatc
     1741 atagaacaca aagcgtaaat actctgtttg tctaccaaat cgtttttcta atgattcaaa
     1801 ctttcttcca caacaaaaaa ttttaatgtt aaaattcctt atttcattta gcaataataa
     1861 ctggtcaatg acacttgaac caatcaaacc agaagctcca gtaattaaaa ttgacttgcc
     1921 attcaagtca ttaatattga cgattgtttc tagagaagat tctacatctt gtctatatat
     1981 tttatttaat aataatgtca ttactattta atccacttat ctcgagttga actcaataag
     2041 gctttaaaaa tatcgagatc ttctacagtc gtaattttca agtttttctc tgttccaaga
     2101 gagaaataac tcttttcacc aaatagttgc atgagttggc atgatgctgc aacatcagtt
     2161 atcccatttt ctaatgcttt ttcatgaagc gatagaatat tatcaagatg gtaaacatga
     2221 ggagtttgtg ttcgtctcaa caagtcacga ttcaatgctt ctgtggacga agttgattgt
     2281 gggttttcta aaacaaatac tacctcagtc gttggtatcg ctgcaacagc atttccaaac
     2341 tgttggtatg ttgctaaagc gttgctaata atatcattgc tcactaaagg acgattcccg
     2401 tcatgaatta atacagtcac atcctcattg ggatgagctt tcttaacttc tctaagaccg
     2461 ttataaattg attcttgacc agacttccct cccaaagtta tcatctttaa ctttgtaatt
     2521 ccaaattgtt ccgcataagc tttaaccatc tgctcccagc cttttaaaat gacaatacag
     2581 atttcatcaa tttctggatg attttgaaat ttttcaaggg tatagataat gactggctta
     2641 ttttcaacat gaataaattg tttagggata tcttgaccaa ttcggcttcc aatgcctgaa
     2701 gctgttaaaa tcgctacttt catttattct tctccgtaac tcaactctaa aaacaaatgt
     2761 tgctctctct tatcaacagg aggaacgacc atataatctt taccgtaaaa catttctaaa
     2821 tactcttctg accaaacagg aacattcacc atcaaagttt caaaaggaac ctttttataa
     2881 atattcatat tctctcgcaa gtaagtctcg cgtctaaagt gtttccttcc tgtaggtatg
     2941 gatacatatt ttgtatgtga atccttacag cttgaattac atttatcaag ccaattcaac
     3001 cacttttcaa ttggaataat tccaaaaaaa caaccaacca ttaactttcc agttgaataa
     3061 tttagttttc taccttccag tctaaacatt tccttcatca gggcatgatt ctttgtttct
     3121 cgtacagaag ccaaggcaaa agtcaaaaac atacttgtat aaccttgaaa gaatcgtttc
     3181 actggattat tgtatacatt ttctacaatg taaagatcta tagaaatacc gcactcctca
     3241 gccgaatgat cgtatttcct acgagcaact gttccttttt ttctaatttg agaaacatga
     3301 agtcccaatt ctggatgact ttttgctgat tgaagataat atttacgact aagactattg
     3361 tctgactcaa atagagaaaa tagtttatcg taactttccc tcggaatatt gatatcaaca
     3421 tcatcatccc aagggataaa ccctttatga cggattgccc ctaaaatgct acctccactt
     3481 aaagaatacg ctatcccatt ttcatcaaaa aaagtaaaca catctgatat cattttcaac
     3541 aattctgatt ggtatttttt caatccgtca tcatctaaaa atgtaatatc tgatttatca
     3601 tattttaatt ttactgccat taactctaac ctttaaatac tttctcgtag tctgtgacaa
     3661 tcttttccca agtgaaataa tccgcaattc tctgatttga ttgtctatct aactcatcaa
     3721 tcatttttgc atcaaattgt tcaactttct cgattacttg ggaaagctca tcttttttcc
     3781 aatagatcgc actttgctcc ccaacttcgc ggttaaaacc aacatcgagt agtaagttta
     3841 actttgtgga tgctaatgct tcaagtaacg aaggatttgt tccaccaact tcatgtccgt
     3901 ggaagtaagc aaaagcattt tctcgaatat acttcagaag ctcttgctca tagactgtac
     3961 ctacaaattt tactcgtggg tccttatcaa aaccagtttt tgccaacaac tgattataaa
     4021 atttattctg ttctacattt gtgattaaga caaagtcctt cttagagtta gacgccaaaa
     4081 agccacgaat catagattca tagttgtttt ctggtacaaa acgtcctaca actagataat
     4141 actcgttctc agaaacattc ttctctttga accaagaacg tactttttca tcactactct
     4201 ttaatgtaga gcgtgttgta tcggtcccat aagcgatata ggttgtctta ggttgatact
     4261 gtatataatc ttcttggata tatttttcaa tattcttact atcacacact aaaagagctg
     4321 catgtttgac cataagacct tcggaaattt tccaataacg acgaactgga gcgctccact
     4381 tagctcgtaa ccactcgtga ccatctggat taactagaag agtcccacct atcgcctgga
     4441 tttttttctt aattccatgg atgaacggac cgattcgaca agctaaaata tagaagattg
     4501 gatcttcatc cttattttct ttggcaattt caatagctct gttaattgca gcgatatcat
     4561 acgctatagc tcgcgctgga ccaatattag gaacatcgac gttataacag atagcaccat
     4621 tatgttcaaa aacatcctca gtagtccctg attttgcaga gttttcacgc atacaagcca
     4681 cataatattg gatagcctta tcttgttgga aggctgttaa tttttcaaca aaagtttcaa
     4741 atcctccata cttagcagga attccttttg aaccaatgat ataaactgac tttttcattt
     4801 ctctcctaat atgatggata tacagaaatt acttcgctcc atctctcata aatacaactt
     4861 taactgtttt caataaaatt tcaatatctt tccagattgt ccaaccatca atataggcca
     4921 catctaattt gacaacttca tcgaaattct tgatttcact tcgtccgctg acctgccata
     4981 aacctgttat gccaggttta aaacttagtc ggcgtttttg ttctggggta tagtgctcat
     5041 actcgtccac tgttggtggt cgtgtaccta ccaagctcat atctcccttt agaacattgt
     5101 aaaactgtgg tagctcgtcc aagctagtct tccgtataaa acgaccaatt ttcgtgatac
     5161 gaggatcatc gtccacctta aacattccac cctgcatggt attttgttcc atgagttctc
     5221 tttttttcgc ctcggcatct acacacatag agcgaaactt ataaaaagtg aactgacgac
     5281 cattttttcc tatacgcgtc tgagcaaaaa tagcagagcc cccatccttt cgaatcaaag
     5341 gaaccagtac aatactgact aaaccacata gtatcagccc taccaatgca cccacgatat
     5401 caataatccg cttagcaatt acatgactag tcttataaaa tgttgtagaa aaagtcacaa
     5461 cgtttaatcc tgccatctca cgaatttgct tgttacgtgc taaactacga tcaaaagcat
     5521 ttagattgac tgttacatca attcccatcg tttcaaactg agagacaagc tctccaatat
     5581 tgtatttttc acttggaaga ttgataaaga cttcatcgac cacctcatga gtcgcaaagt
     5641 ttactatctc cccctctgct actaccttta aacaatcatg ctgaaaatct ggtttatcta
     5701 agacactgac ggctaccaac tccccaacaa cctcatttga ttctattaat ctatccagta
     5761 ccttttcgac acgagaagtt gctgtaagta ggagaatctt cttacttcct ttaaagttgg
     5821 gataagcccg cttccaatac cacttgataa ataggtttag cacatagact aagagagcat
     5881 gtaatgtgag gaagtaaatc atgcctcgtc tggaaatact aaatcgatcc tctaagaaaa
     5941 aattagaaat actaatcgct agtgcaaaga ataggatata tttcaatgtc tggacaagtt
     6001 caatcaaata tcccctttta aagaaatcct gtccataatc actgatataa aagacaaaat
     6061 aatggaggat ataaagtgca atagctgttg ttgaaacaat ctccgcttct ctcacagcac
     6121 taagtagata agtcaataaa ataacaagaa aactctggat tatggccaat gaagacttta
     6181 ctatttttcc attcatctct atcccccgac ctatttttta tttttcccgt aatctccata
     6241 atttccataa gaaccgtatt tgtctactga agtatcgaat ttattcaaca caactcccaa
     6301 aaacggcttc cctgtgtgtt ccaactgttc ttttgctttt tgaatatccc gtcgatttat
     6361 ttcacctgcc tccgtcacta aaatagaagc atcgcagttt cgcgtaataa tagccgcatc
     6421 aatcacgaca ccaacaggag cagtatctac aatgatgtag tcaaaatatt tacgcaaggt
     6481 ttcaagcatt gtagtgaaat tcttactttg aagaagagct gtcggattcg gtgacacaga
     6541 gccagcctga attacaaaga gattttcgat attggtatca caaagccctt gtgataagtc
     6601 tgtagttcct gataaaaatt ctgtcaggcc tgtaatctta ttccttgctt taaagacacc
     6661 taacataaca gaattgcgaa tatctccatc aatcagcagc gttttgtaac ctgcacgcgc
     6721 aaaagcccaa gcgatattgg cggaagtcgt tgattttcct tctcctattt tcacagaagt
     6781 gatagaaaat actttcaaac catctccact taactgtagg ttcgtgcaca aagcgttata
     6841 atattcctct gcctttttta cagaatccaa ttttgcctgt gagatttcta atgttggcat
     6901 aacttcctcc tattttattt tgtcaaaatc tggaacgagc cctaaaagtg gaattttcag
     6961 tacatcttca acatcttcag gacgtttcac acgggtgtcc agcaactcaa tcaaaagaac
     7021 agcaattact gttacgactg ctcctccaag aaaaccaaac aaggaattgc gtcgaacatt
     7081 tggagaagag ggagtcgtag ctggtcgcgc ttcttcaagt gtcgttacat cagatactcg
     7141 cgttacagcg acgatctttt ctgcagcaac ttctcgtaga gaattagcga tacgactggc
     7201 ttcctctggc tgtttatcct tgacagagat tgagacgata cgagtgtcag ctggtacagt
     7261 cacttgaact ttgctggcta acgtttttgc tggcatatcc aacttcaaat ttgtcgctac
     7321 tttttccaat gcatcctgcg aaaggataat ctcacggtag tcttttacca gataagatcc
     7381 tgcctgcaaa tcctgatttg tcagccccgg cttgtctcct tgattgcgat tcactacgta
     7441 aattcgcgtg gtactcgtat attctggctt aacaataaaa gtgctatatg caaaagcccc
     7501 cgcacctgtc acaagtgcca ctattaaaat cattagcttg cgtttccaca aggttttaag
     7561 taattgaaat acatcgattt ctatcgtgtt ttgttctttc atcatttctc ctaaattagt
     7621 tgatccatta caatttttcg aggattgtct ataaaaagtt cctgagcctt cgcttctccg
     7681 tatttttggg taacaaggtc atatgcttct gccatatgag gaggtctacc gtctagattg
     7741 tgcatatcac ttgcaatgat atgaaccaaa tcctgctcta aaaaatactg agctcttttt
     7801 ttcatgaatt tataacgttc gccaaaaagt ttgggtttga ggacatgtga actatttact
     7861 tgcgtgtaac agcccctatc aatcagttct cgaacgcgtt tttcattatt ttcaagagca
     7921 tcatagcgct caatgtgggc aatgactgga gtaattccca acatcaagat cttgctcaag
     7981 gcgctatgaa tatcgcgata aggagtgttc atactgaact ctatcaaggc ataacgacta
     8041 tcattgaggg tcggaatccg ctttttttcc agcttatcca gaacatctgg tgtgtagtaa
     8101 atttcagccc cgtaagcaat gaccaagtca ctcgccactt ccttagctat ttcccgaacc
     8161 tgaagaaagt tttctgctat cttctcttcc ggagtttcaa acatgccctt gcgacggtga
     8221 gaggtagaga caatggttcg caccccctgc ctgtaggatt ctgtcaagag agccttgctt
     8281 tcctctcttg acttgggacc gtcatctaca tcaaaaacga tatgcgaatg gatgtctatc
     8341 atttcatcta ccctccatca catcctgtat agctgcttta actacagcta aactactatc
     8401 atctatttcc atcacataga ggttactgtc tggcattgca taagaaggaa gatccgtccg
     8461 acctgtacct tttaaatctt gagaatttac tttataattc cctccacttt ctaactgagc
     8521 attgaccaaa tttatcatag tctcaagtgg catatttgtt tggatagaat cttgcaagct
     8581 attaatgatc gtgctataat ttttcagtgc ttcggttgac gttaattttt gaaggatagc
     8641 tacaatcacc ttttgttgat tgcgcccgcg gtcacgatcg ccatctgcta gtgagtagcg
     8701 ctcacgaaca aaaccgagag cctgttctga atcaagatga acattgcctg cagggtaata
     8761 ctttccattc gtatgggcag taaattcttg atcattataa acatcaattc cacccaacaa
     8821 atcaatcaat ttcaaaaacg aagtgaagtt caatcgcaca tagtaattga tatccactcc
     8881 atagagattt tctaaggtgt gaatggacga atcaactcca taaatgcccg catgggttaa
     8941 tttatctttt tgattatttc caccatctgc gattggtaca taggcatcac gtggcgttgt
     9001 ggtcaagagg attttcttgg tatctcgatt gacagtcatc aggatattga catctgatcg
     9061 cgacaccgaa ctaatagggc cataggtgtc aattccacta acatagatat tgaaagactg
     9121 atttttagac gtcttaggag cttctacttt tttagtgaat cccttggtat aaatcttttt
     9181 tatcttcgat gcgtagtctg gatactctga ctcgatgata ttttcaaaga cactatttag
     9241 gacaatggcc ttagtctccc ctgcaatcaa actcttgtaa gctgccaagt aagacgaact
     9301 ctggttgacc gtcaaatcgg tattctgact tgacttgata tcagctagta gtttttgaat
     9361 attttcatta tcagtcccag tcggtgctgt cacactcgtc agttgcgtaa cattttcgat
     9421 atcactatct gctaaaacag cgacactgat tgaatattct gagtaattag aagtcgcatt
     9481 taaacgattg gtcagtccaa caaactgctg tactgcaaag agcgacacag agctgacaag
     9541 gatagagaac agcaacagaa aaatagtaaa tttttcagct tttttataga taatcaagag
     9601 tagccctacc aaggcaacta gtaggactaa cgcagttacc actagattaa gatatctaaa
     9661 agcaaggata ttgtacttaa agattaagaa caataaaaaa caaactaaca ataaataaat
     9721 agtcagcaaa actatattaa cacttcgctt cactttctgt gaacgtgatt ttttaaaacg
     9781 tctactcatg attaacacct atacattgaa catcatacga ttatatcact tttttacggt
     9841 aatgtctaca cctttatttt cactatctgc atctttaagt atcttagtag acttcccgcg
     9901 aaccaaaaat ataataaaat gaaataagga cagtcaaatc gatttctaac aatgttttag
     9961 aagcagatgt gtactattct agtttcaatc taatatagta cacaatgtgg tataatcttt
    10021 ttatggcata ttcaatagat tttcgtaaaa aagttctctc ttattgtgag cgaacaggta
    10081 gtataacaga agc
//
LOCUS       DBERMR010000074         9755 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.074, whole genome shotgun sequence.
ACCESSION   DBERMR010000074 DBERMR010000000
VERSION     DBERMR010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9755
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.074"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            171..755
                     /locus_tag="ACL8A1_001987"
                     /pseudo
     CDS             171..755
                     /locus_tag="ACL8A1_001987"
                     /inference="COORDINATES: protein motif:HMM:NF021048.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
     gene            752..1942
                     /locus_tag="ACL8A1_001988"
     CDS             752..1942
                     /locus_tag="ACL8A1_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HGS1202694.1"
                     /translation="MTVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIM
                     YSFEDTWTSIEDYKEFLSVRLEECKRVLKNSGSIFVHCDKIANHHIRLILDNIFGADM
                     FQSEIIWNYKRWSNSKKGLLNNHQNIYFYSKSKDFKFNTIFTEYSSTTNIDQILVERK
                     RDGNSKTIYKVDNNGNYILAKEKNGVPLSDVWNIPFLNPKAKERVGYPTQKPILLLEQ
                     IIKIATDKNDIVLDPFCGSGTTLVASKILNRNYMGIDLSEEAINITQQRLENVIKTSS
                     NLLNKGIEAYRTKTEEEENILKLLQAKIVQRNKGIDGFLPKHFQKKPIPIKIQKNNEC
                     LNESISLLQNAINSKKLDFGVVIKTHSDNLLFDFDTIPENIIVVDHFELTIEKWLSKS
                     QQLL"
     gene            complement(2457..3449)
                     /locus_tag="ACL8A1_001989"
     CDS             complement(2457..3449)
                     /locus_tag="ACL8A1_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS1202695.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYW
                     FYEGGSFIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIVSANGEKFNPYQTITANSL
                     CTFDFDNIKDDFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQPNQI
                     ILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNRKKYKIEIDDDKAL
                     EDVARFLDDHFATNVNQGTLEKISKMSGTKLKNLFKEKYCQSITEYTQRKRMNVAETL
                     LLNTELPIKEIAESVGYSSASKFSIYYKRYKGKLPSEVRNLACKHHNFKCDYCD"
     gene            3609..5363
                     /locus_tag="ACL8A1_001990"
     CDS             3609..5363
                     /locus_tag="ACL8A1_001990"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1202696.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISAALTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIVTPILVLVLGFIVSIRVGI
                     TLLALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNY
                     NIEFENVSFAYNDKAVIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            5347..7068
                     /locus_tag="ACL8A1_001991"
     CDS             5347..7068
                     /locus_tag="ACL8A1_001991"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1202697.1"
                     /translation="MKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLYKKMEDSERVHLKAEVTTILTLSISSIFSFISLAVVIFVGVNLIINKEINSLYLI
                     GYLLAAMKIKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKFDIDLKD
                     VEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQILIDGK
                     DIKEISTESLFDKVSIVFQDVVLFNQSIMENIRIGKQDASDEEVKRAAKLANCTDFIE
                     KMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNEKKIQES
                     LNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNLIEKTK
                     MAEEFIY"
     gene            7079..7663
                     /locus_tag="ACL8A1_001992"
     CDS             7079..7663
                     /locus_tag="ACL8A1_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HGS1202698.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALVALGAFLGGILGAYIGKALLKKHFEKAGIV"
     gene            7663..8358
                     /locus_tag="ACL8A1_001993"
     CDS             7663..8358
                     /locus_tag="ACL8A1_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HGS1202699.1"
                     /translation="MPTFSSNAPFNPNPISKILVVFITGLTVMHSINIRFELAIVCLI
                     GILLYLNGYKKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIPIVIRMFILPFMA
                     ASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKNIKMAMRVRGISF
                     KNPVKYLEYVSMPLLIISSNISDDIAKAAETKAIENPIAKTRYIRVKIQLIDFVYVLA
                     VAGLIVGGLIWLK"
     gene            8346..9710
                     /locus_tag="ACL8A1_001994"
     CDS             8346..9710
                     /locus_tag="ACL8A1_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1202700.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGNGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDMLKIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDEPSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLIDKGKLK
                     KTYTRSEFLKLDKNELNALSLRDKELSKLKVPYLKEGGEYQIKNLSYKFTDDECLSLK
                     DISFKLGKIYGIIGSNGRGKSTLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVM
                     QDVNHQLFTDEVFNELRLGVKNFDEEKAKIILKDLGLDEFIERHPMSLSGGQKQRLAI
                     ASVMCKNSPFIFFDEPSSGMDYSNMIKISELINKYKTMDKIIFIVSHDIEFLNEVADE
                     IFEL"
BASE COUNT         3625 a         1299 c         1663 g         3168 t
ORIGIN      
        1 tagaactttt agaaacaatc aatatttaaa aagaaaaaag ctcaaagaat ttattaaacc
       61 atgttataat agatatagct ataattttat ttataacaag aggatagaaa tgaccgaatt
      121 agaaagaaaa aatcgaaaaa ttagctaaga aatactctga taacttaaac atcaaagttc
      181 aagagagagt tcgtgaaatg gcaaatgata ataagagcca ttatttgata tacagagttt
      241 taggtatttc atttgaagaa ggagaaaata tcgatttgta tcaaaataaa ggtcgttttt
      301 tatacaaata tgctggttca tttttagaag aagctgcagt actatccttt aacgaaaaat
      361 ttggtacaga aaatacttaa aaagttaaca ttcctaattc tgaaagtaca aaacctaaga
      421 cttttgaaat tgattgttta gtcggagaaa aacacgcata cgaaataaaa tggtgggatg
      481 caactacaga tggagaccat ataactaaag aacacactag aataaaagtt attcataaca
      541 aaggatatat accaattcgg ttaatgttct actatccaaa tagaactcaa gccataaaaa
      601 ttcagcaaac tttagaaaca ttgtataacg gtattggagg gaaatattat tatggagatt
      661 ctgcctggga acatttaaga gcagtgaccg gtattgattt acttagtatt ctaacagata
      721 ttgcaaataa aaaaacaggg gtaaaatcaa aatgacagta ttaaaaggag ataacttaga
      781 aatattaaaa actattgaat cctcaagtat tgatttaatc tatatggacc ctcctttctt
      841 tacacagaaa acccaaaaat tatctaataa caaaaatatt atgtattcat tcgaagatac
      901 gtggacttcg attgaggatt acaaagaatt tttgtctgta agattagaag aatgcaaaag
      961 agtgctaaaa aatagtggca gtattttcgt tcattgtgat aaaattgcaa atcatcatat
     1021 tagattaatt ttagataata tctttggagc agatatgttt caaagcgaaa ttatatggaa
     1081 ctataaacgg tggtctaatt caaaaaaggg attattgaac aatcatcaaa acatttactt
     1141 ttattcaaag tcaaaagatt ttaaatttaa tacaattttt acagagtatt cttctactac
     1201 aaatatcgac caaatactag tggaacgaaa acgagatgga aactctaaaa ctatatataa
     1261 ggttgataat aatggtaact atattctagc aaaagagaaa aatggagttc ccctttcaga
     1321 tgtttggaat ataccatttc ttaatccaaa agctaaagaa agagtaggtt atcctacaca
     1381 aaaacctatt ctgttattag aacaaattat aaagattgct actgataaaa atgatatagt
     1441 tttagacccg ttctgtggaa gtggaactac tttagtagcc tccaagattt tgaatagaaa
     1501 ttatatgggg attgatttat ctgaggaagc tatcaatata actcagcaac gtctggaaaa
     1561 tgttataaaa acaagttcaa atttattgaa taaaggaatc gaagcatata gaaccaaaac
     1621 tgaggaagag gaaaacattc ttaaattatt acaggcaaaa attgttcaaa gaaataaagg
     1681 aattgatggt tttttaccta aacattttca aaaaaaaccg atacctataa aaattcaaaa
     1741 aaataatgaa tgtctgaatg agagtatctc tttattacag aatgctataa actccaaaaa
     1801 acttgatttt ggagtagtta taaaaactca ttcagataat ttattatttg attttgatac
     1861 gattcctgaa aatataatag tagttgacca ttttgaactt acaatcgaaa aatggttaag
     1921 taaaagtcaa cagttactat agtttgcaaa ttatttacag aaagaacaaa aaatgctctg
     1981 aaacactgat ttcaaagcat tttttgttag tttaaaatta ctcccattct tcttttccaa
     2041 acgtacaata tatccagaac cattcaaaat actagattct attttttata atatcactaa
     2101 atccacctaa ttataggacg ttttcagatt tttagtcccg tcccagtccc agtaccggag
     2161 aaatattgtt ttaatataat atctgttttt gtcttctaag ctcttaaaag caaaagaaca
     2221 agtaaagagt caagacaagg ataaaaagtc catattaggg caaataaaaa gctttaagac
     2281 agatgacaaa tctaagtcaa ataagaaaga ccatagcaaa ggggcagaga gataaatatt
     2341 ggcggtcttc ggactgcctt tattttttta tccatttttc aaatcaaatt tattcagact
     2401 atatatgcac atatacactt aaattcatat aaaaacatgg cttgtaaaaa attactttaa
     2461 tcacaataat cgcatttaaa attgtgatgt ttgcaagcta aattacggac ttcacttgga
     2521 agttttccct tgtatctttt ataatagata gaaaatttgc tggcagatga atatccaaca
     2581 gattctgcta tctcttttat aggtagttca gtgtttaaaa gaagagtttc agctacattc
     2641 attctttttc tttgagtgta ctctgtaatg ctttgacaat atttttcctt aaataaattt
     2701 tttaatttag taccactcat tttagatatt ttttcaagcg tgccttgatt tacattcgtt
     2761 gcaaaatgat catctaagaa tcttgctaca tcttcaagtg ctttatcatc atcaatttca
     2821 attttatatt tttttctatt taagtatgtg tcaattacta tacttatcca ttcatttgcc
     2881 tttgctttaa agaaaaaatc agcggcagga gcgtccatct tacaatttaa tatttccatt
     2941 gccactcttt ctaaggcctt tgtaagtatt atttgattcg gttgaagcaa ggttgaataa
     3001 aaagattctg gattaatgtt aatagatgct aaatgttttt ctattagctc ttttttaaaa
     3061 ccaatggaaa cagcaagata acaacaattc tcgtgtaata aaaaaacaaa atcatctttt
     3121 atattatcaa aatcaaaagt acataaagag tttgcggtaa tagtttgata cggattaaac
     3181 ttttctccgt ttgcactgac aatgtaactt gaataaattg aaacatagtc tgacatacta
     3241 taagtgctat tttgaactac ttcctctttg atataaaaat catgtatatc gataatgaag
     3301 ctgcctcctt cataaaacca atataggcct tctgcataag ttgaattctc ttttttccaa
     3361 caaaatgtat gacctgcact tgaatacttc tcattgtttt tacattcatc tactttcata
     3421 taatctttta caaaatcata atatgacata acatactatc ccttttagac aatattccaa
     3481 ttagccttat taattcaaaa ctattgtatt agtaattata acagatgtat aatagaaaag
     3541 caatgataga tattatcaat tcagcgaatt tatatctaaa agggatatta aagaaaggag
     3601 atatgcttat gaagatttac aaaaaactat ttgcttatgt ccaagataag aaatatcttg
     3661 gggttttggc cataattttt tctgctatat ctgctgcact tacagtatat ggatattatt
     3721 taatctacaa atttctagat aagttaataa ttaattcaaa cttatccggt gcagagagta
     3781 tagcattaaa atctgttatt acactaacaa gtggagcgat attttatttt gtctcaggaa
     3841 tgttttcaca tatcttggga tttaggcttg aaacaaattt aagaaaaagg ggaatcgatg
     3901 gtctggaaaa agcaagtttt aggttctttg acttaaatcc atctggtcaa ataagaaaga
     3961 tcatagatga taatgcagca caaactcatc aggtggtagc tcacatgatt ccagatagct
     4021 ctcaggcaat agtcacacct atacttgtcc ttgtactagg ctttatagta agtataaggg
     4081 ttggtataac tttgcttgct cttactataa ttggcggctt aattttaggg gcaatgatgg
     4141 gcgagcaaga atttatgaag atataccaag aatccctatc taaactaagt gctgaaactg
     4201 ttgagtacgt gagaggaatg caagttgtaa aaatatttaa agcaaatgta gagtcattta
     4261 aaagctttta caaggctata aaagattact caaagtatgc ttatgattat tccctatctt
     4321 gtaaaaggcc ttatgttttg tatcaatggt tattttttgg actgattgca attttaatta
     4381 ttcctatagt ttattttatg actagcttag ctagcgcaaa ggtgatttta cttgagctta
     4441 tcatgatttt atttttatca ggagttctct ttgtttcatt catgagaatg atgtggtact
     4501 ccatgtatat ttctcaagga aattatgcag tagatacttt agaggcgctt tacgaagata
     4561 tgcaaaaaga caaattagtg catggtaatg tcaataattt taaaaactat aatatagaat
     4621 ttgagaatgt tagctttgct tataatgata aagctgtcat tgaaaattta tcctttaatt
     4681 tagaagaagg aaagtcctac gcacttgtcg gttcatctgg atcaggcaaa tcaacagtag
     4741 caaaacttat atcaggtttt tacaatgtta ataaaggaag cataaagata ggcgggatag
     4801 caataagtga atattctgac gaagccttaa ttaaagccat ttcctttgtt tttcaagatt
     4861 caaaattatt taagaagagc atttatgata atgtagcttt agctaataaa gatgcgacga
     4921 aagatgacgt tatgagagcc ttaaaattag caggatgcga tttaatatta gacaaattcc
     4981 cagaaagaga aaatacaatc ataggctcaa aaggtgttta tttatccggt ggagaaaaac
     5041 aaagaattgc aattgctaga gcaattttaa aggattccaa aattattatt atggatgaag
     5101 catcagcatc tattgaccca gataacgagt ttgaattgca aaaagctttt aaaaatctta
     5161 tgaaggataa aacagttatc atgattgcac acaggctatc tacaattaaa gaccttgatg
     5221 aaattattgt catggatagt ggaaaaatta tagaaagagg gtctgacaaa gaattaatgt
     5281 caaaagatac aaggtataag agcctgcaag agatgtttaa cagtgcgaat gaatggaggg
     5341 tttcaaatga aagagtttta taaaaaaaga tttgctctta cagatggagg agcaagaaat
     5401 ttaagtaaag caacactggc ttcatttttc gtttattgta taaacatgct tcctgccata
     5461 ttacttatga tttttgctca ggaagttttg gaaaatatgg gcaaaagcaa tggcttttat
     5521 atagtattct cagttttgac tttgatagca atgtatattt tgctttctat cgaatacgat
     5581 aaattatata acacaaccta tcaagaaagt gcagatttaa gaataaggac agcggagaat
     5641 ttatcaaaat tacctctatc ttacttttct aaacatgaca tttccgacat ttcacaaaca
     5701 atcatggctg atattgaagg catagagcat gcaatgagcc actcaatacc aaaggtgggc
     5761 ggcatggtac tgtttttccc attaatatct gtaatgatgc tagcgggcaa tgtcaagatg
     5821 ggtttagctg taattattcc atctatttta agctttatat ttataccttt atctaaaaaa
     5881 tatcaggtta atggacagaa tagatattat gatgtcttaa gaaaaaactc agaaagcttt
     5941 caagaaaata tcgaaatgca aatggagatt aaagcatata atttatcgaa ggatattaaa
     6001 gatgacttat ataaaaaaat ggaagatagt gagagagtac acttaaaggc agaagtaact
     6061 acaattttaa ctttgtctat atcttcaata tttagcttta tatctcttgc tgttgtgata
     6121 tttgtcggcg taaatctaat tattaataaa gagataaatt ctctctacct tataggatat
     6181 ttactagctg ctatgaagat aaaagactct ttagatgcat ctaaagaggg cttgatggaa
     6241 atattttatt tatcgcccaa aatagaaaga ttaaaagaaa ttcaaaatca agatttacaa
     6301 gaaggcgatg actatagctt aaaaaaattt gatattgatc taaaagatgt tgagtttgcc
     6361 tacaataaag acgcaaaagt tttaaatggt gtaagtttta aagctaagca gggagaggtc
     6421 actgctttgg taggtgcaag tggctgcggt aaaacaacta tcttgaaact tatatcaaga
     6481 ctttatgatt atgacaaggg acaaatctta atcgatggca aagatataaa agaaatatca
     6541 acagaatctc tttttgataa ggtctctata gttttccaag atgtggttct ctttaatcaa
     6601 agcattatgg aaaatattag aatcggaaag caagatgcaa gtgacgaaga ggttaaaaga
     6661 gcagcaaaac ttgcaaattg cacagatttt atagaaaaaa tggataaagg tttcgataca
     6721 gttattggtg agaacggagc tgagctatca ggaggagaaa gacaaagatt atcaatagcc
     6781 agagccttct taaaagatgc gccgatattg atcttagatg agataacagc aagccttgat
     6841 gttaacaacg agaaaaagat tcaagagtct ttaaataatt tagttaaaga taaaactgtt
     6901 gtaatcattt cacatagaat gaaatccata gaaaatgcag acaagatagt agttcttcaa
     6961 aacggaagag tagaaagcga aggtaagcat gaagagcttt tacaaaaatc aaaaatttac
     7021 aaaaatttaa tagaaaagac aaaaatggca gaagaattta tttattagga ggactacaat
     7081 ggataataaa aaattaaaag taaaagattt agtaagtatc ggtgtttttg gcgtaattta
     7141 ttttgccttc atgtttggag ttggtatgat gggcttgatt ccaatattgt tcttaatata
     7201 cccgacagta ttagccatag ttgcaggaac tgttgttatg ttatttatgg ctaaggttca
     7261 aaagccatgg gcactattta tatttggtat gatatcacca cttgtgatgt ttgcagctgg
     7321 tcatacctac gtagttgtgg ttttatcact tatagtaatg ataatagcag aattaattag
     7381 aaagattggt aattataatt catttaaata caatatgctt tcttatgcaa tcttcagcac
     7441 atggatatgt agctctttaa tgcaaatgct tttagcaaaa gaaaaatata tggagtggtc
     7501 tttgatgacg atgggaaaag attatgttga tgtattagaa aagttaataa cttatcctca
     7561 catggcttta gtagccttag gtgctttctt aggaggaatt cttggagcat atataggcaa
     7621 ggctctattg aaaaaacact ttgaaaaagc aggcattgta taatgcctac tttttcttca
     7681 aatgctccct ttaatcccaa tccgatctct aagatattag tcgtatttat cacaggctta
     7741 actgttatgc atagcataaa catccgtttt gagctggcaa ttgtttgtct tattggtatt
     7801 ttgctttatt tgaatggata caaaaaaaca cttttcaaat ggattatatt gtgtgggata
     7861 ctttactcct tgcctaattt tatggtgtta tctgaattaa accctatagt taagatgttt
     7921 ttgagtatac ctattgttat tagaatgttt attttaccat ttatggcagc aagctttatg
     7981 ataaagacct cggatgtagg tgcaataatt tcatcgatgg ataagcttaa gatttcaaag
     8041 aatgtatcca tacctattgc ggttatgttt agattcttcc catcttttaa ggaggagaag
     8101 aaaaacatca aaatggctat gagagtaaga gggataagtt ttaaaaaccc agtcaaatat
     8161 cttgaatatg tttctatgcc actactcatt atatcatcta atatatcaga tgacattgca
     8221 aaagcggcag aaacaaaggc aatagaaaat ccaattgcca agaccagata cattcgcgta
     8281 aagatacagc taattgattt tgtttatgtt ttagcggttg ctggacttat tgtgggaggc
     8341 ttaatatggt tgaaataaaa aatttaagtc ttgattatgg tgaagagcat atattagatg
     8401 atatatcact atccatagcc gagggagagt gcgtgctatt tacaggaaaa agtggaaatg
     8461 gtaagtcatc tttaataaat tcaatcaatg gactagctgt aaggtatgat aacgcaaaga
     8521 caaagggcga aataattatt gatggtaaga atataaaaaa tttggaactt tatcaaatct
     8581 caatgcttgt ttcaactgtt tttcaaaatc ctaagacata tttttttaat gtcaatacga
     8641 cattagaatt attattttat ttggaaaata tcggtcttgc aagagaagag atggacaggc
     8701 gtttgaagga tatgcttaag atattcccga taaaaaatct tttgaacaga aatatattta
     8761 atctatccgg cggtgaaaaa caaattcttt gcattgcagc ttcttatata gcaggtacaa
     8821 agattatagt tatggatgag ccttcatcga atttagatat taaaagcata agtgttttgg
     8881 caaagatgct aaagatatta aaagagaaag gcataagcat aattgttgca gagcatagaa
     8941 tttattattt gatggacata gttgaccgtg tatttttaat agataaagga aagcttaaaa
     9001 aaacttatac tagaagtgaa tttttaaagc tagataaaaa tgaattaaat gctttaagtt
     9061 taagagataa agaattaagt aaattaaaag ttccttattt aaaagaaggt ggagagtatc
     9121 agataaaaaa tcttagctac aaatttactg atgatgagtg tttaagctta aaagatattt
     9181 cattcaagct tgggaaaatt tatggcataa taggatccaa cggacgagga aaatcaacgc
     9241 ttttaagatg tttaataggt cttgagaaaa aatcaaaaga agaaatttat tttaagggag
     9301 agaagctatc taaaaaagaa agactcaaaa actcttcact tgttatgcaa gatgtaaatc
     9361 atcaattatt cacagatgaa gtattcaacg agcttagatt aggagtaaag aattttgatg
     9421 aagaaaaggc gaaaatcatt ttaaaagatt taggcctgga cgaatttatt gaaaggcatc
     9481 cgatgagttt atcaggaggg caaaagcaaa ggcttgcaat agcatctgtt atgtgcaaga
     9541 attctccatt tatctttttt gacgaacctt caagtggtat ggattattcc aatatgataa
     9601 aaatatctga actgattaat aagtataaaa ccatggataa aataattttt attgtttccc
     9661 atgatataga atttttaaat gaagtggcag atgaaatttt tgaattgtaa aatagagtct
     9721 gttaaacatg tctatagaac tagtaaattg tctaa
//
LOCUS       DBERMR010000075         8802 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.075, whole genome shotgun sequence.
ACCESSION   DBERMR010000075 DBERMR010000000
VERSION     DBERMR010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8802
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.075"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(370..1977)
                     /locus_tag="ACL8A1_001995"
     CDS             complement(370..1977)
                     /locus_tag="ACL8A1_001995"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HGS1202701.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(2367..2954)
                     /gene="rpoE"
                     /locus_tag="ACL8A1_001996"
     CDS             complement(2367..2954)
                     /gene="rpoE"
                     /locus_tag="ACL8A1_001996"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HGS1202702.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(3082..3393)
                     /locus_tag="ACL8A1_001997"
                     /pseudo
     CDS             complement(3082..3393)
                     /locus_tag="ACL8A1_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
     gene            complement(3291..3707)
                     /locus_tag="ACL8A1_001998"
     CDS             complement(3291..3707)
                     /locus_tag="ACL8A1_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202703.1"
                     /translation="MKVNIVDLHPTQLYLSEKKLQDIQMLYQSAETNQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLKNHI
                     LPLWIGMVSFFGLKSRRRENGFQLGILHYLRIISPL"
     gene            complement(3735..4385)
                     /locus_tag="ACL8A1_001999"
     CDS             complement(3735..4385)
                     /locus_tag="ACL8A1_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HGS1202704.1"
                     /translation="MKDKQTFLMKGSFALLLFVILGYMVKFYPETLVGFDQSIQTAIR
                     GDLPDYLTILFRSLTHLIDIPVIITWVVIIAFVFYRKRWKIESFFMLGNLALAGLLIV
                     TFKNIYQRPRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDQVWRKIVQI
                     VLGLYLVSVLVSRVYLGVHYPSDVLASFCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(4375..4938)
                     /locus_tag="ACL8A1_002000"
     CDS             complement(4375..4938)
                     /locus_tag="ACL8A1_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGS1202705.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTVGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(5103..5337)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(5391..5894)
                     /locus_tag="ACL8A1_002001"
     CDS             complement(5391..5894)
                     /locus_tag="ACL8A1_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HGS1202706.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(6087..6917)
                     /locus_tag="ACL8A1_002002"
     CDS             complement(6087..6917)
                     /locus_tag="ACL8A1_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HGS1202707.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            complement(6902..8584)
                     /locus_tag="ACL8A1_002003"
     CDS             complement(6902..8584)
                     /locus_tag="ACL8A1_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1202708.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQTAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLADLDNLSIS
                     KGQVQLQTELVKETPALQSLFRLEEVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
BASE COUNT         2655 a         2008 c         1605 g         2534 t
ORIGIN      
        1 attttactat agtggattaa attaaaaaag tataatgtat tgaatctata acagtacatc
       61 ttgactgcta aaatatttct ataaattaat ttgacttttc tgatagagat gttcacatct
      121 tatttcaatt cactatacaa tccagttcct aaaactttgc tagaaattga tttggctttc
      181 cgaattaatt tgttcaaatt ttatttcatt tcaatatact gataaatcca ttcactagca
      241 cagggcaaga gggagtcgct attgatttca acggtagcag gtacatacag aggaccccta
      301 cctcagatat actgcaatat catcgtatgc aaaaaacctc tgagattttt ctcaaaggtt
      361 ctgattttgc taattgctgt tctcaaccgc tgcagtaaca aaggcagtgt agagttcttc
      421 tggtcggttt ggacggcttg acagttcagg gtgatactga caagctacaa agaatttatt
      481 ttcaggaatt tccacgattt ctaccaaacg attgtctgga gaaactcctg aaaagacaaa
      541 acctgctgcc tcaaactgct cacggaaggc attattaaac tcataacggt gacggtgacg
      601 gcgttgcacc acttcttgat tgtgataagc agcagcagcc ttagagccac gtttcaactt
      661 agacggataa agtcccaaac gaagggttcc acccatatcc tcaatatcaa tctgatcacg
      721 catgatatca atgatagggt attttgtttc tggtgcaagc tctgcagaat tggcaccttc
      781 aagacctaaa acgtgacgag caaactcgat acatgtcaac tgcattccca agcagactcc
      841 caacattgga acatcatttt cacgcgcata gcggatggct tggattttcc cttctgtacc
      901 acgttgacca aaaccacctg gtacgatgat cccgtccgca tcagacaaga gttctgctac
      961 attctctgct gtcacatcat tggcattgac ccaattgatt ttaacttcta catcattgac
     1021 atagccagag tgtttcaagg cttcgaccac tgagatatag gcatcttgca actccacata
     1081 cttaccaaca agggaaatct taacttgttt cttgaggttc atgaccttgt ccaccatggc
     1141 tgaccattct gtcatatccg ctgctggtgc gtctaatttc aaatgatcac aaacaatttg
     1201 gtccatccct tgtgcctgca agttcagtgg aatttggtaa aggtgttcaa catccaacga
     1261 ttcgataacg gcttctggtg ccacatcaca gaactgggcc agtttatttt taattccttg
     1321 accagctggc tcttctgtac gaataaccaa catatttggt tggattccca agccacgcaa
     1381 ttctttgaca gagtgttggg ttggtttggt tttcatttca ccagcagcct tgaggtaagg
     1441 aagcaaggtt gtatggatat acatgacatt atccgcaccc acatctgcct tcatctgacg
     1501 aagagcctct aggaatggca aggactcgat atctcctact gttccaccaa cctctgtgat
     1561 aatgacatca gagtcggtcg ttagagcggc acgcttgatt ttttctttca aagcatctgt
     1621 gatatgagga atgacttgaa cagttgcccc aaggtattct ccacggcgtt ctttacgaag
     1681 aacttcactg taaattttcc cagttgtcac gttggaatat ttgttgagat tgatatcgat
     1741 aaaacgttca tagtgaccca agtccaaatc tgtctcagct ccgtcatctg tcacaaaaac
     1801 ttccccgtgc tggtaaggac tcatggttcc cggatcaata ttgatataag ggtcaaactt
     1861 ttgaatggtt actttgagac cacgattttt caagagacgg cctagactcg ctgccacaat
     1921 ccctttccca atagacgata ccacaccacc agttacaaaa atatatttcg tagacataga
     1981 ttcctctttc taaaatgctc aaggtcttgt taactacgag gggatataga aaacaagacc
     2041 ctactaataa gaataatctg ggacgactgc accagattca caactaatac tcttcgaaaa
     2101 tctcttcaaa ccgcgtcaac gtcgccttgc cgtagatatg tgttcctgac tttgtcagtc
     2161 ttatctacaa cctcaaaaca gtgttttgag cagcctacgg ctagtttcct agcttgctct
     2221 ttgattttca ttgagtataa aatacaagaa gataacttct tgaaaaaaca aaaatagctc
     2281 cctaagaact agggagcccc gacctctaaa agaggtgccc aaacaatatg atacctaaaa
     2341 ataggataat tgtcaatagc taactcttat tcctcgctgg tttcagcatc gtcatctgaa
     2401 aactcgtcgt cgtcttcgtt gagatccaca tcttctccca agtcatctgg agcgatttcg
     2461 ttgatttctg catcataagc ttccacttca tttttttcat catctggatt ttcatcatcg
     2521 tatgaaagag ctggatctgc ttcgtatgca tcttcgtctt ctggatcatc tgcattgtag
     2581 tcaatggcat ctgaatcacc atccataaag gcattgacac gttttttctt agcttttggt
     2641 gctacttcat cgtcgtcatt ttcttcaaga gcgatgattt cttcgtcgat ttcgtccaca
     2701 ccataccatg aacgaagacc ccatttgttg tccccaagtg agatgaagct accgtcaaag
     2761 ttcaactctg tgtagaacaa aggcaaagct tcgcggatat cgctgtttga tgttccaagg
     2821 tagttttgaa tttcgtttac aagatcgcta aaatgcatct catgatcgcg accacgaagt
     2881 tccaagatag cacgcgctac ctcaatcata gatagttcac ttttttcttg cccagcaaat
     2941 acttctaatt ccaaagcgtt tctcctcatt tatactacta tcgccagagc gaacagactc
     3001 tgacctcatt ttatcattta ctctttattt tacgataatt ttgcggaata gtcaaaggtt
     3061 aagggggaga aagtggcagg attagactaa ttccaatata aaactccttc ctttttctgt
     3121 tgctccattt tccacaaatc caagcgactt gaaacacctc ctagaagcat gattgtaggt
     3181 gtagatttcc ttgactctca attctttcca tccttttact cgagccaatt caatcaaagc
     3241 acttagaatc ttttttccaa gtcctcgatg ttggtaagcg gaattcccaa tcacaatggg
     3301 gagattatcc tgagatagtg taatatcccc aattggaaac cattctccct tctccttgac
     3361 ttcaatccaa aaaagctcac catgccgatc caaagaggta agatatgatt ttttaaatca
     3421 aaaacagagt aaatctttct ttcctcgcaa gcttgcgcat agagatgata tagttcatca
     3481 ccaccatctc tatcccactc agcagaaatc gtatcccgac ctgccaataa agcctgataa
     3541 gccctgtgat gcccatctgt aatcagcaaa caatctccaa aggcaagaat actgattgga
     3601 tcgacttggt ttgtttctgc cgactggtaa agcatctgaa tatcttgcaa cttcttttct
     3661 gataaatata gttgagtcgg atgaagatct actatattga ctttcatttc tttctcctca
     3721 agggaattcg atactcactt ctgtttgcct ttaaatcgcc attggaagcg gagcttgtca
     3781 tagaagggaa actcgataaa caagactccc aaacccacac agaaactggc aagaacatct
     3841 gatgggtagt gaactcccag atagactctt gataccagca cactgactag gtagaggcca
     3901 aggacgattt gtacgatttt tctccagacc tgatctttaa tccgctgact aagaataaca
     3961 atcaaagtcc ctaccatcag cgttacagct agagaatgcc cacttgggaa ggaaaatccc
     4021 ttctcctcca ccagatgtaa gatagctggt cgtgggcgct ggtagatatt tttaaaggtc
     4081 acgattaaaa gacctgccaa agccagattt cccagcatga agaaactttc tatcttccat
     4141 cgcttacgat aaaagacaaa agctataatg acaacccaag tgataatcac tggaatatca
     4201 atcagatgtg tgagggatcg aaaaagaata gtcaaataat ctggtaagtc tcctcgaatg
     4261 gcagtctgaa tcgattggtc aaaaccgacc agcgtttcag ggtaaaattt gaccatatag
     4321 ccaagaataa cgaaaagtaa aagggcaaaa ctgcccttca ttaaaaatgt ttgtttatct
     4381 ttcatagtgt tttaaggttg gtttcaaaag gacgtacaac aaccagaatg aaacggaaaa
     4441 gattacaccc tcaatcaagt taaaaggtaa taccatggtc attaggtagt tggaaagtcc
     4501 caaaattttt ccaatatcaa agttagcaaa cttagcgtac aaaggaacag cataaacata
     4561 gttgagaacc aacatggcca aggttaaacc aacagttcca gctagagagc ctagtaggaa
     4621 acgaagggtt gtccgttcct ttttccaaat caaagcaaat acgatgacaa aaactcccaa
     4681 agctacgata ttcatcggca aaccaatgta agtattcact ccttggctgt taagaagcaa
     4741 tttcaagagt gagcgaagca agagcactcc tagagcagca ggcaaatcca tgaccaccag
     4801 acccacaagg actggcaaga tactaaattc gatcttgagg aaagatgccg ctggtaaaag
     4861 cggaaagtca aagtacatca gcacaaatga gatggctgat agaattgcaa tggtcgaaag
     4921 tcgacgtgtg tttgtcataa caggttcctc caattttcta taaaatcaga agaagttgga
     4981 aaggattcct ctatctattc tcacttttta tatcccaaaa gttccctctt actctattaa
     5041 agaaaaacaa agcaagtggt tacaatccgg ctataaatct atcaaaacag acaaagctat
     5101 tctttcgtct tctcccatcc agactatact gtcggttgtg gaatctcacc acatcagctt
     5161 gcgctcgcgg acttctttaa agagaaggaa atagagattt ttcttttaga ctaggcgaca
     5221 agctgtaggc ataacttctg gttaggcaaa gcttgtcaac aaagtataaa aggaaaagat
     5281 catttcctag ttaaagtcac cgccggtcgg gaatcacacc ctgccctgaa gactctttta
     5341 tcataacaaa aaacgcttgc aagcgcaagc attttgttca ttttcatttt ttatttcaat
     5401 ttatccactt cataggtatg gacgagctca agacctgcaa agttctgctg gcgaagagcc
     5461 tcatagacaa tcatgcagac ggtattagac acattgagac tgcggacatg ttcatcattc
     5521 atgggaatac ggagagcctt ctcaggatgt tctcgcataa aatcttcagg taagcccttg
     5581 tcttcacgtc caaagagaaa ataatggtcc tcgtcagtcg ataaatccac ctcagaatag
     5641 actttttccg cgaatttcga aatcagatag agttttccct tcatctgaga tatgaaatct
     5701 tccaaactct cgtaaaaata aatctctagc ttatcccagt aatccaatcc agcccgcttc
     5761 atcttgcggt catcaatagg aaagcccata ggcttgatga tgtggagggg agaattggtc
     5821 gcagcgcaag tacgcgcgat attgcccgta ttttgtggaa tttgaggttc aaataataca
     5881 atgtgatttg tcatgacttg cttcctttca ccattgcaaa aaaatagcca cactgcccgg
     5941 agtcaagctc agcaaacagc gtggttaagg catcgttaac ttacctcaca acaggtttga
     6001 agtaaatcag cgaaactact ttcttagtat aacactttca gaatcattgt caatagaaat
     6061 gacttgattt tttcaatttt ttcaagctat ttccaagggt tgtaaaatcg tccctgattc
     6121 tgcaagataa gtagtaaact aactactaaa aacaaggttg ccaagagcaa ggtaatatag
     6181 tctccttttt tcaaggcctg ataactatac catgtgcgtt ttttctcttt cccaaagcgg
     6241 cgaagctcca tggcagtcgc aatggtatca atgcgttcta gcgagctaaa aatcaagggc
     6301 gtaataatga gcagattgcc tttgattcgt tgcataagag aagctttctt ggataattcc
     6361 atcccacgcg cctcctgaga catcttgata gtaaagaatt cttcctgcaa atctggaata
     6421 tagcgcaagg tcaggctgac agaataagca atcttatagg gcacaccaat ttgatttaaa
     6481 ctggaagcaa actgactagg atgggttgtc atcaaaaaga taatagccag aggaatggtg
     6541 caaagatact taatggccaa atttagcaga taaaagagct cctggctggt tagagtgtag
     6601 acaccgattc cctgccaaat cacacttctc tctccataaa gtccaacccc atactcggga
     6661 gaaaagagat agaccatcaa aacgtttaaa acggcaaata ccgtcgcaaa aacggctaca
     6721 aaggaaacat ctttaaagcg aatttctgat aaatagagga gaaagactga aaagatggca
     6781 atcagcaaga gcattctggt atcatagcta atcatggccg ccaatgatac cagaatgaaa
     6841 aagagaagtt tcccagctcc tgacaagcga tgaatcacag tatctctatg ctggtaaccg
     6901 attaatttag cttgcatccc tctctccttt ctttgtaaaa tgccgttaaa tccagtggat
     6961 ccacatctag tttcttagcc aagttaaaga tggaggtttc ttttagattg gcttttacta
     7021 acagctcagg atcgctcaac agactggctg gaacagtatc ggcaatcaat tctccatcca
     7081 ccatgacaag gacccggtct gaataatcca gcatcaattg catatcatgg gtaatcatga
     7141 caatggtatg ccctttttga tgtaactctt cgagaaattc cataatctca gtatagttct
     7201 tctgatcttg acctgcagtc ggttcatcta ggagaataat ttcagctcct aagaccaaaa
     7261 ttgaagcaat ggtgacacgt tttttctgac caaatgacag ggcagaaata ggccaattac
     7321 ggaattcata aagtccacag attttcaagg tttcatatac tctcgtttca atttccttct
     7381 catccacacc tcgcaaacgg agccctagag ccacctcatc aaaaatcata ttggttgaaa
     7441 tcatttgatt aggattttgt agcacatatc ctactcgttc cgcccgctct gcaacagaat
     7501 cgccttttat atcctgtttt tcccaaagat agcgtccttc cgtctgaata aagctactta
     7561 tagccttggc tagagttgat ttccctgctc cattttttcc gacaatagca atcttttcac
     7621 cctttttaat atctaaatgt agggatttta aaatcggtct atcatcataa gaaaaagata
     7681 cttcctctag tctaaagagt gactgcaatg ctggggtttc ttttaccagt tccgtctgta
     7741 actggacttg cccttttgag atagacaagt tatccagatc tgctaattgt tcttccttga
     7801 ctaagtccac acctaattga cggagagtcg ttagataaag gggttctcga attccatttt
     7861 gagtcaataa atcagtcgca agcaactggt cagggctccc attaaaaagg atacgaccat
     7921 cgtttatcaa gacaatccga tccacagggc gatgcagaac gtcctccaaa cggtgctcga
     7981 taatcagagt cgtcgtcccc tcttccttat gaatctggtc aatcaattcg ataatatcct
     8041 gacctgactt gggatctaga ttggcgagtg gctcatcaaa caagagaatc ggactttcat
     8101 caatcaagac accagccaga ctgactcgct gcttttgtcc acctgacaaa tcctgaggac
     8161 gctgatccag taaaggaaga aggtccagct tttcagccca tttataaaca cgacctttca
     8221 tctcatctag ggctgtcaca tcattttcca gagcaaacgc caaatcttct gccacagaca
     8281 agccaataaa ctgcccatct gtatcctgca aaactgtgct aaccagatga gacttatcat
     8341 agatgctcat atcaaaggct gtttgaccct ttatcaaaaa ttctccatat gtctgaccct
     8401 tgtaaatatt gggaataatc ccattcaaac actgacccaa ggtagattta cctgacccag
     8461 atggtccaac aattaagact ttctctccct tgtaaatggt caagtctatc ccttgcaagg
     8521 tcggttcttg ttgtgtttca taccggaaag agaaatcctt ccactcaatt atagcttctt
     8581 tcatcttact ctcttcattc gcttcttaga cttctatttt atcataaatc aagcccttct
     8641 tgcagtctct tctcttaaaa tcttagcgcc aaaaagattc ctatcctagc ttacttgcct
     8701 aactaatcta taaacatcga aaaagactag ttgcccagcc ttccccatca ttttatactc
     8761 ttcgaaaatc tcttcaaacc acgtcagctt cgccttgccg ta
//
LOCUS       DBERMR010000076         8609 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.076, whole genome shotgun sequence.
ACCESSION   DBERMR010000076 DBERMR010000000
VERSION     DBERMR010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8609
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.076"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            184..1203
                     /gene="galE"
                     /locus_tag="ACL8A1_002004"
     CDS             184..1203
                     /gene="galE"
                     /locus_tag="ACL8A1_002004"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HGS1202709.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTATLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            1205..2170
                     /locus_tag="ACL8A1_002005"
     CDS             1205..2170
                     /locus_tag="ACL8A1_002005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGS1202710.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTHYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKNKVKRL"
     gene            complement(2221..2427)
                     /locus_tag="ACL8A1_002006"
     CDS             complement(2221..2427)
                     /locus_tag="ACL8A1_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HGS1202711.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            2479..2955
                     /locus_tag="ACL8A1_002007"
     CDS             2479..2955
                     /locus_tag="ACL8A1_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HGS1202712.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            2964..3635
                     /gene="cmk"
                     /locus_tag="ACL8A1_002008"
     CDS             2964..3635
                     /gene="cmk"
                     /locus_tag="ACL8A1_002008"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HGS1202713.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            3766..4104
                     /locus_tag="ACL8A1_002009"
     CDS             3766..4104
                     /locus_tag="ACL8A1_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HGS1202714.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            4107..4736
                     /locus_tag="ACL8A1_002010"
     CDS             4107..4736
                     /locus_tag="ACL8A1_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HGS1202715.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITKHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            4976..6127
                     /locus_tag="ACL8A1_002011"
     CDS             4976..6127
                     /locus_tag="ACL8A1_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGS1202716.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            6440..7189
                     /gene="truA"
                     /locus_tag="ACL8A1_002012"
     CDS             6440..7189
                     /gene="truA"
                     /locus_tag="ACL8A1_002012"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HGS1202717.1"
                     /translation="MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            7179..7952
                     /locus_tag="ACL8A1_002013"
     CDS             7179..7952
                     /locus_tag="ACL8A1_002013"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HGS1202718.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            7930..8391
                     /locus_tag="ACL8A1_002014"
     CDS             7930..8391
                     /locus_tag="ACL8A1_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGS1202719.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
BASE COUNT         2434 a         1616 c         1933 g         2626 t
ORIGIN      
        1 gatagaactg acgaagtcag taaccatacc catggcaagg cgacgttgac gtggtttgaa
       61 ttggattttc gaagagtata agcattttac tgaaatttta tcaggtagtt taggatggca
      121 aatgacattc cctgtcaaaa atggtaaaat aagaaaaaat aatctgagaa tcgaggaaaa
      181 aagatgcaag aaaagatttt ggtaactggt ggtgccggtt ttattggaac ccacactgtt
      241 attgaattga tccaagcagg ccatcaagtt gttgtggtgg ataatcttgt caatagcaat
      301 cgtaagagtt tagaagttgt tgaaagaatc acaggagttg agattccttt ctatgaggca
      361 gatattcgtg acacagctac cctcagagat attttcaagc aggaagaacc gactggtgtc
      421 attcactttg ctggtttgaa ggctgttggc gaatcaacac gtatccctct tgcctactat
      481 gacaacaata tcgctggaac tgtcagcctt ttgaaagcca tggaagaaaa caactgtaaa
      541 aacatcatct tcagttcttc tgcgacagtt tacggggatc cgcacacagt gcccatcttg
      601 gaagatttcc cactttcagt gaccaaccca tacggtcgta ctaagctcat gctagaggaa
      661 attttgactg atatttacaa agcagactca gaatggaatg ttgtcttgct tcgttacttt
      721 aacccaatcg gagcccatga gagtggtgat ttgggagaaa atccaaacgg tattccaaac
      781 aatctcttgc catatgtgac tcaagtagcc gttggaaaat tagagcaagt gcaagtgttt
      841 ggagacgatt acgatacgga agatggaaca ggtgttcgtg actatatcca cgttgtcgat
      901 ttggctaagg gtcacgttgc agctttgaaa aaaatccaaa aaggttcagg actaaacgtt
      961 tataaccttg gaactggtaa aggttactca gttcttgaaa ttatccaaaa catggaaaaa
     1021 gcggtgggac gtcctattcc ttaccgcatc gtagaacgtc gcccaggtga tatcgctgcc
     1081 tgctattcag acccagcaaa agctaaagca gaactcggtt gggaagcaga actcgacatc
     1141 acccaaatgt gtgaagacgc atggcgttgg caaagcaagc atccaaatgg atttgaagac
     1201 taagatgatg atttcaatca tcgtcccttg tttaaacgaa gaggaagtac ttcctctttt
     1261 ttatcaggct ctggaagctt tacttccaga tttggaaaca gaaatcgagt atgtctttgt
     1321 cgatgatgga tcaagtgatg ggaccttgga actcttaaag gcctatcggg agcaaaatcc
     1381 ggcagtccat tatatttctt tctctcgaaa ttttggcaaa gaagcagccc tttatgcagg
     1441 cttgcaatat gcgacaggag atttggtggt ggtgatggat gcagacctcc aagatcctcc
     1501 tagtatgttg tttgagatga aaaatgtact agacaaaaat gtagacttgg actgcgttgg
     1561 gacacggaga actagtcggg agggagaacc cttctttcgc agtttctgtg ctgttctctt
     1621 ttatcgcctc atgcaaaaaa tcagcccagt agctctgccg tcgggtgtcc gtgattttcg
     1681 tatgatgaga aggtctgtgg tcgatgccat tttaagtttg actgagtcca atcgtttttc
     1741 taagggactc tttgcctggg tcggctttaa aacccactat ctggactatc caaatgtcga
     1801 aaggcaggct ggcaagacca gttggagttt taggcaactc tttttctact ccattgaagg
     1861 gattattaat ttttcagatt ttcccttgat tatagccttt gtagctggtc tcctatcttg
     1921 ttttctttct ctgctgatga ccttttttgt tgtggttcgg accctcattt tgggcaatcc
     1981 gacatcgggt tggacctctc tgatggctgt cattctcttt cttggaggca ttcaactctt
     2041 gaccattggg attctcggca agtatatcag taagatttat ttagagacta aaaaaagacc
     2101 actttatctt atcaaagaaa aaagtgacct tcctgatttt acagaaaaaa ataaagtgaa
     2161 aagactataa ttttacatgg aaatgtgcta aactagaggg agtggattat cgccattgat
     2221 ttaggcttcc tggtttccaa tcagggcgcg agttgggcaa tttttaacag cctctaagac
     2281 atcctgacta ggagaaattt ctttttccag ttggtcaggg tcatcgtaaa aacgcacgat
     2341 tccattatcg tggtaatcaa ataaatcaga ataagtttgg caaagcccac aggcgataca
     2401 tcgttcaggt ataagtgtga ttttcatgtt catatttata ttgtaataag aaaagtaaaa
     2461 aaaaacaagg agtaaggtat ggcaaaagaa ccgtggcaag aagatatcta tgatcaagaa
     2521 gaatcaagag cagagcgtcg gcatcgaaac cacggagggg ctgataggat ggctaatcgt
     2581 attttgacga tcctagctag tattttcttt gtaattgtgg tggtgatggt catcgttctc
     2641 atctatctat catcgggggg aagtaatcgc acagcagcct taaaagactt tcatgattct
     2701 gatgcaagtg tagtacaaat ctcatcttca agcagttctc agcctgagca gagttcagag
     2761 ccagaatcta cttctagtag ttcagaagaa gctgctaatc ctgaaggaac gattaaagtt
     2821 ctcgcaggag aaggggaagc agctattgcc gctcgtgcag gaatctccat tgctcagtta
     2881 gaggccttga atcctgggca catggctaca ggatcttggt ttgctaatcc aggtgatgtt
     2941 ataaaaataa aataggagcc gatatgaaaa caattcaaat tgctattgat ggtcctgctt
     3001 ccagcggtaa gagtacggtc gcaaagatta ttgctaagga ttttggattc acctaccttg
     3061 atacaggagc tatgtatcgt gcagcgacct atatggctct taagaaccaa ttaggagttg
     3121 aagaagtcga agcccttcta gccttgttgg accagcatcc aatcagcttt ggacgttcag
     3181 aaactggaga ccagcttgtt tttgtaggag atgtggatat tacccatcct atccgtgaaa
     3241 atgaagtgac caatcatgtt tctgctattg cagcaattcc tgaagtgcgt gagaaactgg
     3301 tttctctcca acaagaaatt gcccagcaag gcgggattgt catggatggt cgcgatattg
     3361 gaactgttgt attgccacaa gcagaattga aaattttcct agtagcttct gttgatgaga
     3421 gagcagagcg tcgttacaag gaaaatattg ccaagggaat tgaaacagac cttgaaaccc
     3481 taaaaaagga aattgctgcg cgtgactaca aggatagtca tcgtgagact tctcctctca
     3541 aacaagcaga ggatgctgtc taccttgata caactggttt gaacattcaa gaagtagttg
     3601 aaaaaatcaa agcagaagct gaaaaaagaa tgtaaatgta gaaaagccga tagattgata
     3661 tcggtttttt tctaaatttg tcaaaatgct gattttaggg tataatagtt gctgttcgag
     3721 aaattttgat tttcaaagaa tagtgaatga tgataaggag aaaccatgaa caatttacca
     3781 aattgtccaa aatgtaactc agagtatgtc tacgaagacg gtgccctact ggtttgccca
     3841 gagtgtgctc atgagtggaa tcctgctgaa gttgcagaag tagaagaagg tcttgtcgct
     3901 atcgatgcca acggaaataa attgactgat ggtgatacag taactctcat caaggacttg
     3961 aaagtaaaag gtgcgccaaa agatttgaaa caagggacgc gcgtgaaaaa tatccgcatc
     4021 gtagaaggcg accacaatat cgactgtaag attgatggtt ttggtgccat gaaacttaaa
     4081 tcagagtttg tgaggaagat ttaagcatgt caaaacacta taaacttgta ttttatagcc
     4141 gtatcttctt gtttctagcg gcttttacgg gagtttatct tgaaattact aagcatggtg
     4201 gttttgggat gcttctctat tacacggttc tgtccaacct cttggtaact atttttaccc
     4261 tttatcttct aaaggttatg agccgtgtag gtgaaaattg gcaaagacca agtctcttgc
     4321 gcttaaaagg tggggtcacc atgagtatca tgattacctg tgtgatttac catttcctct
     4381 tggcgcccat tgcgactaat ttctataccc tagaaaattt cctttgccac tatatcgttc
     4441 ccatctggtt tttagcggat accctctttt ttgacaaaca gggtcaatac aagatttggg
     4501 atccagcagt gtggaccatt ttaccctttc tgtatatgat gtttgctctt tttaatggct
     4561 tggttctaaa actcaatatt ccaaatgcca aggataatcc tttcccttac ttctttttga
     4621 atgtgaacaa gggttggaat gttgtgttta agtggtgtct gattatcttt gttgcctata
     4681 tggtagcagg atttattttc tactttatca agcaaatcaa gagaaagtca tcctaatact
     4741 cttcgaaaat ctcttcaaac cacgtcagtt tccatctgca acctcaaaac actgttttga
     4801 acaacctgcg gctagcttcc tagtttgctc tttgattttc attgagtata agatacaagg
     4861 gattcatttt cgaatttaag aaggagtcga caaaccaatg aggcttcttc ttttcttgct
     4921 tatttgataa ctatcaaaaa agtagaagat taagagaaat atagaaagag atttcatgaa
     4981 acaattttta gaacgggcca gcattttggc tctctccctc gttttgatta cctccttttc
     5041 catttcgagt gccctaccag ccatgtttga ctattatcag ggttattcta aggaacaaat
     5101 tgagctcttg gtgagcttgc cttcctttgg aatcatgatg atgttactgc taaatggttt
     5161 cttagaaaaa atatttcctg agcgcttaca gattagtttg ggcttgctga ttttatcatt
     5221 gagcggtaca gctcccttct ggtaccaagc ctatcccttt gtctttggaa cacggcttct
     5281 ctttggtttg ggtcttggga tgatcaatgc caaggccatt tctattatca gtgaacgcta
     5341 ccaaggaaaa aggcgaattc agatgttagg gctacgcgct tctgcagagg tcgttggagc
     5401 ttctctcatt accttggccg tcggtcagtt gttggccttt ggttggacag ctatctttct
     5461 agcctatagt gctggatttt tggtgctgcc cctttatctg ctctttgtcc cttatggaaa
     5521 atcaaagaaa gaagtcaaga aaagagcgaa ggaagcaagt cgtttaactc gagaaatgaa
     5581 aggcttgatt tttaccttag ctatcgaagc ggcagttgta gtttgtacca atacagctat
     5641 taccatccgt attccaagtt tgatggtgga aagaggattg ggggatgccc agttatctag
     5701 ttttgttctt agtatcatgc agttgatcgg gattgtggct ggggtgagtt tttctttctt
     5761 gatttctatc tttaaagaga aactgctcct ctggtctggt attacctttg gcttggggca
     5821 aatcgtgatt gccttgtctt catccttgtg ggtggtagta gcaggaagtg ttctggctgg
     5881 atttgcctat agtgtagtct tgacgacggt ctttcaactt gtctctgaac gaattccagc
     5941 taaactcctc aatcaagcaa cttcatttgc tgtattaggc tgtagtttcg gagcctttac
     6001 gaccccattt gttctaggtg caattggctt actaactcac aatgggatgt tggtctttag
     6061 tatcttagga ggttggttga ttgtaatctc tatctttgtc atgtacctac ttcagaagag
     6121 agcttaggat tgattcctaa gtttttcttt tgatactcaa tgaaaatcaa agagcatagt
     6181 tgattgagtt tggaatagta cgctgtagct gctaaaatat ttctagaaat taatttgact
     6241 tttctaatag agttattcat atcttatttc aatctactat agaaagctag ccgcaggttg
     6301 ctcaaaatag tgttttgagg ttgcagatgg aaactgacgt ggtttgaaga gattttcgaa
     6361 gagtatgaga tttttatacc catacaatat ttgttttagg tccttgttta cagactaatt
     6421 tcttgataaa atagaggtta tgacgagata taaagcaact atttcctatg atggttatgc
     6481 ctttgctggc tttcagcgcc agtctcatgc gcgtagcgtt caggaagaaa ttgaaaaaac
     6541 cttgaccaga ttaaataaag ggcaaaccat tactgttcac ggtgctggta ggacagatag
     6601 tggggttcat gccctgggac aggtcattca ttttgacctg ccttatcaga tggatgagga
     6661 gaaactccgt tttgccttgg atacccagtc tcctgaagat attgatgtga tttcgattga
     6721 gcttgtggca gatgattttc attgccgtta tgccaaacat agcaagacct atgagtttat
     6781 tgtggataga ggacgtccca aaaatcctat gcgccgtcac tatgccaccc actttcccta
     6841 cccactcgat gtggaacgaa tgcagattgc aatcaaaaag ctagagggaa cccatgattt
     6901 taccggtttt acagcctctg ggactagtgt agaggataag gttcgcacca tcacagaagc
     6961 tagtttaata gtcgatgaga caggacaatt tttgaccttt accttttcag gaaatggttt
     7021 cttgtataaa cagattcgca atatggtggg gactcttctc aaaatcggta acaaccgcat
     7081 gccagtagag cagattgatc tcatcttgga gaagaaggac aggcaacttg caggtcccac
     7141 tgcagcacca aatggtttgt atttaaagga gattcgttat gaagaataat cgtattttag
     7201 cactttctgg aaatgatatt tttagtggtg gtggactgtc agctgatttg gctacctata
     7261 ccttgaacgg cttgcatggg tttgtagcag tgacttgttt gacagccttg acagaaaaag
     7321 gatttgaagt ctttccaact gatgatacca tttttcaaca tgaattagat agcttacgtg
     7381 atgtggaatt tgggggaatt aagattggtc ttctccctac tgtcagtgtg gctgagaagg
     7441 ccttggactt tatcaaacaa cgcccaggag tacctgtggt gttggatcct gtcttggtct
     7501 gcaaggaaac gcatgatgta gctgtcagtg agctctgcca agagttgatt cgctttttcc
     7561 cttatgtcag tgtgattacg cctaatctcc cagaagcaga attattatcc ggtcaggaaa
     7621 ttaaaacctt ggaagacatg aaaactgcag cgcagaaatt gcatgattta ggagcgccag
     7681 cagtcattat caagggaggc aatcgtctta gtcaggacaa ggctgtggat gtcttttatg
     7741 atggacagac ctttactatc ctagaaaatc cagttatcca aggccaaaat gctggtgcag
     7801 gttgtacctt tgcctctagc attgccagtc acttgattaa aggtgataaa cttttgccag
     7861 cagtagaaag ctctaaggct ttcgtttatc gtgctattgc acaagcagat cagtatggag
     7921 taagacaata tgaagcaaac aaaaacaact aaaatcgccc ttgtatccct attaaccgcc
     7981 ctttctgtgg ttctaggtta tttcttaaaa atcccaacac ctacaggaat tctaactctt
     8041 ttagatgctg gtgtcttctt tgcggccttt tactttggta gtcgtgaagg agcggtagtc
     8101 ggaggactag caagtttctt gattgacctc ttatcaggct accctcagtg gatgttcttt
     8161 agcttggtca accatggctt gcagggattt ttcgcaggat ttaaaggaaa aagtcagtgg
     8221 ttaggcctta ttttagcaac tattgccatg gtaggaggct acgccttggg ttctactttg
     8281 atgaatggct gggcagcagc cctcccagaa attctaccaa atttcatgca aaatatggta
     8341 gggatgattg taggatttat tcttagtcaa agtatcaaga agattaagta atactcttcg
     8401 aaaatctctt caaaccacgt cagcttccat ctgcaacctc aaaacactgt tttgagcagc
     8461 ctgaggctag ctttctagtt tgcactttga ttttcattga gtataaagag gttgagtaaa
     8521 aagtatttca aataagaaaa acgcatagta tcaggtgttg aatgaactgc cccccaaaag
     8581 ttagacagaa aaaatctaac ttttggggt
//
LOCUS       DBERMR010000077         8278 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.077, whole genome shotgun sequence.
ACCESSION   DBERMR010000077 DBERMR010000000
VERSION     DBERMR010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8278
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.077"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(98..346)
                     /locus_tag="ACL8A1_002015"
     CDS             complement(98..346)
                     /locus_tag="ACL8A1_002015"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1202720.1"
                     /translation="MASTSLQAPAKICFILIKLYSPYAFLSKYLGAILLSFFCYYAKE
                     IGCNNLTLSVWNDNEGALRFYQRQGMKPQETTMEMIID"
     gene            complement(294..1376)
                     /locus_tag="ACL8A1_002016"
     CDS             complement(294..1376)
                     /locus_tag="ACL8A1_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HGS1202721.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTDFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTATDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(1502..2284)
                     /gene="gatD"
                     /locus_tag="ACL8A1_002017"
     CDS             complement(1502..2284)
                     /gene="gatD"
                     /locus_tag="ACL8A1_002017"
                     /EC_number="6.3.5.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0140282 - carbon-nitrogen ligase activity
                     on lipid II [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit GatD"
                     /protein_id="HGS1202722.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(2284..3627)
                     /gene="murT"
                     /locus_tag="ACL8A1_002018"
     CDS             complement(2284..3627)
                     /gene="murT"
                     /locus_tag="ACL8A1_002018"
                     /EC_number="6.3.5.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_function: GO:0140282 - carbon-nitrogen ligase activity
                     on lipid II [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HGS1202723.1"
                     /translation="MNLKTTLGLLAGRSSHFILSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILEAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECILVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(3726..5042)
                     /locus_tag="ACL8A1_002019"
     CDS             complement(3726..5042)
                     /locus_tag="ACL8A1_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HGS1202724.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQSLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            5235..6461
                     /locus_tag="ACL8A1_002020"
     CDS             5235..6461
                     /locus_tag="ACL8A1_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HGS1202725.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGA
                     GIVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            6660..8234
                     /locus_tag="ACL8A1_002021"
     CDS             6660..8234
                     /locus_tag="ACL8A1_002021"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGS1202726.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQNIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
BASE COUNT         2339 a         1621 c         1852 g         2466 t
ORIGIN      
        1 cattctacac ccactattat gcgttttatt gtgggaagat tttcctcatt ttctagtgaa
       61 attgaacttt ttctaacatc ttttgataga tgacttctta atcaattatc atttccattg
      121 ttgtttcttg gggtttcatc ccttgtcttt gataaaagcg gagagcaccc tcgttatcat
      181 tccagacact tagggttaga ttattgcagc caatttcttt agcataatag cagaaaaatg
      241 acaataaaat agcccctaga tattttgaaa ggaaagcata ggggctatat agtttaatca
      301 aaataaagca aatctttgct ggtgcttgta aagaggtcga agccatcctt ggtaacaaca
      361 ccgcagtctt caatacgaac accgacttta ccagggatat agatacctgg ttcaacagag
      421 aagcacatgc cttcttcgat gaccatgtcg tttccttcca tgatagatgg gaattcatgg
      481 acatccatac cgataccatg cccgagacgg tggttgaagt actcaccata accagctttt
      541 tcgatgacct cacgggcagc gcggtccact tcatgagcag tcacacctgg cttgataaag
      601 tcaagagcag cttgttgggc ttcaagagtc aagttgtaaa tatctttctt gaattggtct
      661 ggtttgccga cagcgactgt acgagtcata tctgacgcat agccattgac cagaacaccc
      721 aggtcaaaga ggagaagagc atcattttca accttattag ctgctggaat gccgtgtgga
      781 ttcgcagcat tatcaccagt caagaccatg gtatcaaagc tcatttcgta accttcacgt
      841 ttcatggcaa agtcaatttg tgcgatgata tctgtcgcag tcttatcaag agaaatattg
      901 tcaaaaccaa catgaacagc cttgtcagca taaagacctg caaccatcat tttttgcact
      961 tcatcagctg atttgatgag gcgcatgcgt tggatacgag gagtgaggtt gtcaaactca
     1021 gcagtctcaa aaactgtttt caaaccatgg tatttggtca agatgagatt gtcaaactca
     1081 acagcgacac gtttgaagtc aagttgtgga agagcatgtt tgattttttg ccatggattt
     1141 tcagaatcga catagcccac tactgggaag gaaacggtgc tacttgcacg ttctacttca
     1201 agagctggga caaagaggag aggttcctga tctgctagga caaagaggaa catttggcgt
     1261 tcatggggat cactgtaaaa atcagtgagg taattgattg tgacggggtc agatacgaca
     1321 gcgacgtcta gtttttctga ttcaagatat gttaggattt gttgtaattt agacatatgc
     1381 taccttcttt ctacccctct attttggcaa aaaatgagag aaaatgcaag ggagaagggt
     1441 aaaagaaaag acaaaaaact ccgacaagat aacggagttc tttgatatca ttttcctttg
     1501 tttaagaaaa gtcagccttg cttttgacgt cactgtactc ttcagcgatt tcttggctga
     1561 gaatgtcctc ataggcaggg agttggatgt cctgaccata tttcttcttg agggcagtag
     1621 taactaggcg ataagccaga ttggcattac gagagaggat aggcccgtgg aagtaggaac
     1681 caaagacatt cttataatga accccttcac cgaccttttc ttcgttgttt ccatttccat
     1741 agacaacctg tcccagcggt ttttggtcat cagagaggaa ggtacgacct tggtgatttt
     1801 caaatccata gtaggtttca tcgaaatctt cattgtgaat cttgatgtca ccgataaaac
     1861 ggttattggt ctggttgagc gtgtagtgtc ccatgacccc tagcccttcg atacgttttc
     1921 ctgaagcttc aacataatat tgacccaata gttggaaacc accgcagata gccagaacta
     1981 caccgtcgtt ttggatgtag ttgtcaatgc tctctttttt agcaggtagg tcgtctgcaa
     2041 tgatactttg ttcaaagtct tgaccaccac cgaaaaaggc gatgtcgtag tgattttcat
     2101 caaagtcatc atggagagaa acgatgtcaa cggtcacatg ggctcccagt ttttcagcca
     2161 catacttgag catgaggatg tttccattgt ccccgtaggt attcatgaga tttccgtaga
     2221 ggtgggcaat gttgagctga taggggtaat tgccatcttt tgaggaaagt gaagtataaa
     2281 ccattagttc atctcctttc taacaatctg acgactagcc agcagttcac gaaattccag
     2341 catggcagta taagttgcca gaatataggc atgcttgcag tcttgattct caatggtctt
     2401 gagaacttgc tccagattac tcgtttcagt gattttctca gctggatagc cagtcactcg
     2461 gaggcgacga gcgatttcag aatgacgaac accgccagcg ttgatttcag gaatgtccat
     2521 gtcagtgatt tgctcaaagt ctgcatccca gatccagcta gtgtcaattc catctgcata
     2581 gttagcatta aggaggacag ataggctaaa tggataaggt gctagtttga tcatttcgat
     2641 agcttgggtt gcaccgactg gatttttaat caagacaagg atacattcct tgtcaccgat
     2701 atgaaaggtt tcttggcgtc caaagacagc acggctcttg tcaaatccct gtttgatgag
     2761 ttgggaatct gcgccgagga aacgggcgat ggccacagca gctagggcgt tatagatatt
     2821 atagagcccg ccgatttgga taccgtattc ttggccgtct atgacaaagc gagagcgatt
     2881 gttggtcaac tcaaccagtt ttgtcaaacg atagtcgaga tcaggacgtt tacatccaca
     2941 accttcacag atataggcac ccaagtttgc ataggtatta tgctcatatt tgaggatgcc
     3001 ttggcagtca ggacagagaa tcccttcggt attgtagtga gccagttggg ctggtccttt
     3061 ttccaagtca aaaccaaaat actctatagg gtttggaata gttggcttgt agaaaagtgg
     3121 actgtctccg ttaaggagaa cagtagcagt tggaactttc cgaatggctt ccaatatcat
     3181 gttataggta gtatagattt caccgaaacg gtccatctgg tcacggaaga tattagtaat
     3241 gacaaaaaga ctaggctgga tatagtcaca gatacgagat agactggctt cgtcaatttc
     3301 gaggacggca atatttttcc cagtttttga agatttggct gttaggaagg ttgttgcaat
     3361 ccctgtaatc atgttggcac cgcttgggtt ggttagaact tgaccataaa cctcttttaa
     3421 aatgccgaca gtgagggcag ttgtcagggt ttttccattt gttccagtga caacgacaat
     3481 ctcgtagttc ttagctaggt tttgtaaaat atctttatca aattgaaggg cgactttacc
     3541 tgggagcgta cttccacggc caagacggct taaaatgaag tgggaagaac gcccagcaag
     3601 aaggcccaaa gtagttttta agttcatgtt tctattatat cataaaagag gagaaagaca
     3661 gatttgagat tccgcagaag taaaaacagc ccaaagaggg ctgttcgata cgattaaatc
     3721 tcaacttaaa tcgcaaacaa gtcattcaag ttctcagcca aggttgggtg agtgaagatt
     3781 tgttttgtga agtaagtgta aggaatcttg ttgtccatag caacagtgat gatgttgatg
     3841 atttcttgag aaccttctga gaagatgctt gctccaagaa tttcttttgt ttcagtattg
     3901 acaacagctt tgaaggcacc gcgaaggtct ccatttacgt gaccacgagg cattgctgca
     3961 acggggattt ccttaacagc gtatggaagt ttcaaatcag ctgcttggct ctcagtcaaa
     4021 ccaacttgtg aaagtgcagg tgtgatgaac atagtatttg gcacattgag acggtcttca
     4081 agtgtgtagc tgccatctcc agcaaggtag ctgtaaacaa cacggaagtc atcaagtgaa
     4141 atgtaagtaa attgaaggcc accgttgaca tctccaactg caaagacacc aggaacgttt
     4201 gtttgacagt gtttgtctac tttaatagca ccacgttcag ttagttcaat atctgtattt
     4261 tcaagttgaa gtggttctac atttggtttg cgtccagttg cgtagagaag ggcgtcgaaa
     4321 cggtaagttt cgtcttcagt tacgacaagc acttggtcac catcgttttt aatttcagta
     4381 gtatggatat tttgaagcaa ttcaatgcca tcttcttcca tgtattgttt agcaagagct
     4441 gcgatggaag gttctgcacg aggtaggaat gtatccaagg catctaggac tgtgaccttg
     4501 cttccaagtt tgttgtaaag gccggcaaat tcaagaccga tatttccgcc accaaggatt
     4561 ccaagttttt caggtaattt gtccaagctt tggatacctg ttgagtcaaa gatgtttttg
     4621 cttgtagcaa gtccagggat tggcaagacg tttgaaacag caccagtgtt gatgacgatt
     4681 gtttcagcag tcagttcttt cttttcatca ccagcttgga tttcgatgac tttatttgaa
     4741 aggaagtgag cttccgcatc aaagatatct acgcctgtac cagcaacagt cgcatagttt
     4801 ttaccgttga ggcgaccagt gatcgtgttt ttagtagcaa tgacttcttc aaaagacaag
     4861 tccttttcag cagcaactag caaggtttta gttgggatac aaccaatgtt gatacaagtt
     4921 ccaccgtaca tagccttgct gcgttcaacg agggcaactt ttttgccagc tgaagccaat
     4981 ttacctgcta gtgttttacc agctttacca aatccaataa caattaaatc gtatgttaac
     5041 atttatcttc ctcctattcg aatttcattc tagcacaaga aaaaaacttt tgcacaattc
     5101 tgctacggaa aagacagaaa aagataaaaa cgctttccag acaaaaaact agtaattttt
     5161 taacaagctt gataaactaa aatcctatct atttttatat aagatagaat aaatgttcga
     5221 aaatgggagg ttttatgaaa tcgaatcgtt atattattgc ctttgctggg gtcattttac
     5281 acttaatgct gggttcgact tatgcctgga gtgtttatcg taaccctatt attgaaaaaa
     5341 cgggatggga tcaggcttct gttgccttcg cctttagtct agcaatcttt tgtttgggct
     5401 tatcggctgc atttatgggg cgtttggtag aaaaatttgg tccgaaagtc atgggaagtc
     5461 tatctgcttt tctatacgca ggtggaaata tcttaacagg atttgcaata gaccgtcagg
     5521 agctgtggtt gttgtatctc gcttatggca ttttaggtgg gcttggtttg ggagcaggct
     5581 atattacccc tgtgtcgacg attataaaat ggtttcctga taaacgtggt ctcgcaacag
     5641 gtttagcgat tatggggttt ggttttgctt ctttattgac tagtcccata gcgcaacacc
     5701 tcatcgcagg ggcagggatt gtagaaactt tttatatttt aggagcaagt tactttatta
     5761 tcatgctcct agcttcacaa ttcattaagc gtccaaatga gcaagagctt gcaattttat
     5821 cttcttcagg gaaagaaaaa acagcctctt tgacgcaagg aatggctgca aatcaggctc
     5881 taaaaagcaa tcggttttat atgctttgga ttattttctt tatcaacata gcttgtggtt
     5941 taggcttaat ttcagcggca tcgccaatgg cacaggagat ggctggcttg tctacaagtc
     6001 atgcagcagt aatggtgggt gttttgggga ttttcaatgg atttggtcgc ttgctctggg
     6061 cgagtttgtc tgactatatc ggtcgccctc taacctttag tatattactg cttgtcaatc
     6121 ttttcttttc tctctcactt tggctcttta cagattccgt tttatttgta gttgctatgt
     6181 ctattttgat gacttgctat ggagctggtt tttctttgat tccagcttat ctcagtgata
     6241 tttttggaac caaggaattg gccgctctgc atggttatat tttaacagct tgggcaatgg
     6301 ctggtttagc gggacctatt ttattagcag agacttataa aatggctcat tcgtacacac
     6361 aaaccttgtt cgtttttctc attttataca gtatcgcctt ggctttgtct tattatctag
     6421 gtcgttcaat caaaaaagaa agtcaaaaac cgcttacatg attttctgta gaaatggggc
     6481 tatcttttgc tctggtcttg tgttatacta gataggttgc aaagaaaaca gtacttttct
     6541 tttgtggaaa agaagcaaca cgattttata tctagttata tgaaaatacg tcataaaaag
     6601 aaaagtataa actaagccct atagggtggc ttcgcaccac ctttagaaag aagaataacg
     6661 tgaaatttaa tgaattaaac ttgtctgctg atttgctggc agaaattgaa aaagctggtt
     6721 ttgtagaagc tagtcctatc caagaacaaa ctattccttt ggcccttgaa ggcaaggatg
     6781 ttatcggtca agctcagact ggtacaggaa aaactgcagc ctttggcttg cctacccttg
     6841 aaaaaatccg tacagaagaa gcgactatcc aagccttggt catcgctcca actcgtgaac
     6901 tagctgtcca aagtcaagaa gaactcttcc gctttggtcg tagtaaggga gtcaaagtcc
     6961 gttcagtata tggcggatca agcattgaaa aacaaattaa ggctcttaaa tctggtgccc
     7021 atatcgtggt gggaactcca ggtcgcctct tggacttgat taaacgcaag gccttgaaat
     7081 tacaaaacat tgaaaccctt atccttgacg aagcggatga aatgcttaac atgggcttcc
     7141 ttgaagacat cgaagccatt atttcccgtg tacctgagaa ccgtcaaact ttgcttttct
     7201 cagcaactat gccagatgcc atcaaacgta tcggtgttca gtttatgaaa gcccctgaac
     7261 atgtcaagat tgcggctaag gaattgacaa cagaattggt tgaccagtac tatatccgtg
     7321 ttaaggaaca agaaaaattt gacaccatga ctcgtctcat ggatgtggca cagccagaac
     7381 tcgctattgt atttggtcgt accaaacgcc gtgtggatga attgactcgt ggtttgaaaa
     7441 ttcgtggctt ccgtgcagaa ggaattcatg gcgacctaga ccaaaacaaa cgtcttcgtg
     7501 tccttcgtga ctttaaaaat ggcaatcttg atgttttggt tgcgacagac gttgcagcgc
     7561 gtggtttgga tatttcaggt gtgacccatg tctacaacta cgatattcca caagatcctg
     7621 agagttatgt tcaccgtatc ggtcgtacag gtcgtgctgg taagtcaggt caatctatta
     7681 cttttgttgc tccaaacgaa atgggttacc ttcaaatcat tgaaaacttg actaagaaac
     7741 gcatgaaagg tctcaaacct gcaagtgtag aagaatcctt ccaatcaaaa aaacaggtag
     7801 ctctcaagaa aatcgaacgt gattttgcag atgaaaccat tcgtgccaac tttgagaaat
     7861 ttggtaagga tgctcgcaaa ttggctgctg agtttactcc agaagaattg gcaatgtata
     7921 tcttgagtct gacagtccaa gacccagata gccttccaga agtggagatt gcacgtgaaa
     7981 aaccactacc gtttaaacca tcaggtaatg gtttcggtgg taaagctaag ggaggtcgtg
     8041 gaggccgtcg tggggacgac cgtcgagagc gtgatcgccg tggcaatggt cgccgtgatg
     8101 agttcaaaaa aggaagtcgt ggcaacgatc gttttgataa ggaaaaacgt taccgtaagg
     8161 ataataaaaa accacgcaat actttaagtg aaaagcaaac aggctttgtt attcgtaaca
     8221 aaggcgataa ataagaaaaa gcggttcaaa atgaatcgct tttttatata aggagacc
//
LOCUS       DBERMR010000078         6759 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.078, whole genome shotgun sequence.
ACCESSION   DBERMR010000078 DBERMR010000000
VERSION     DBERMR010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6759
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.078"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..338
                     /locus_tag="ACL8A1_002022"
     CDS             <1..338
                     /locus_tag="ACL8A1_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HGS1202727.1"
                     /translation="VAGLTNGELIAPMTYEEMMTSDFFEAWFQKFLLPTLTTPSVIIM
                     DNARFHRMGKLELLCEEFGHKLLPLPPYSPEYNPIEKTWAHIKKHLKKVLPSCNTFYE
                     ALLSCSCFN"
     gene            399..1374
                     /locus_tag="ACL8A1_002023"
                     /pseudo
     CDS             399..1374
                     /locus_tag="ACL8A1_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            1551..2558
                     /gene="gap"
                     /locus_tag="ACL8A1_002024"
     CDS             1551..2558
                     /gene="gap"
                     /locus_tag="ACL8A1_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HGS1202728.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFVKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2625..3500)
                     /locus_tag="ACL8A1_002025"
     CDS             complement(2625..3500)
                     /locus_tag="ACL8A1_002025"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HGS1202729.1"
                     /translation="MQFTFTLPASLPQMTVKQLLEEQLLIPRKIRHFLRIKKHILINQ
                     EEVHWNEIVNPGDVCQLTFDEEDYSQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTH
                     GNQPNEIALLNHVSTYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISR
                     EYWALVDGNINRKELVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLA
                     RCKLKTGRTHQIRVHLSHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLEKLTF
                     TTLSNTFEKELKKNG"
     gene            3587..5782
                     /gene="pbp2a"
                     /locus_tag="ACL8A1_002026"
     CDS             3587..5782
                     /gene="pbp2a"
                     /locus_tag="ACL8A1_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP2A"
                     /protein_id="HGS1202730.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENISLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVVTVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTSGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5835..5987
                     /gene="rpmG"
                     /locus_tag="ACL8A1_002027"
     CDS             5835..5987
                     /gene="rpmG"
                     /locus_tag="ACL8A1_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HGS1202731.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            5997..6173
                     /gene="secE"
                     /locus_tag="ACL8A1_002028"
     CDS             5997..6173
                     /gene="secE"
                     /locus_tag="ACL8A1_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HGS1202732.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            6228..>6759
                     /gene="nusG"
                     /locus_tag="ACL8A1_002029"
     CDS             6228..>6759
                     /gene="nusG"
                     /locus_tag="ACL8A1_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HGS1202733.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAE"
BASE COUNT         2102 a         1181 c         1463 g         2013 t
ORIGIN      
        1 tggttgcagg tctaacaaat ggtgagttaa tcgctccaat gacttacgaa gagatgatga
       61 cgagcgactt ttttgaagca tggtttcaga agtttctctt accaacatta accacaccat
      121 cggttattat tatggataat gcaagattcc atagaatggg gaagctagaa cttttatgcg
      181 aagagtttgg gcataaactt ttacctcttc ctccctactc acctgagtac aatcctattg
      241 agaaaacatg ggctcatatc aaaaagcacc tcaaaaaggt attaccaagt tgcaatacct
      301 tttatgaggc tcttttgtcc tgctcttgtt tcaattgact atagttcacg gatacagttg
      361 ggaaagaagc tattaaatat ttggagagtc cttttgggat gaacatgtca aatattacta
      421 tagatgggca agaaaagatg ggcaagaaat ggatctgagt caaacgatgc ttcttatgct
      481 caagtagtga gtctttatga tgatacaagt atctctgtta gtaataatga gacggacaaa
      541 gtgttgactg gatccctcta tactgaaaca aatgaacagg gattaacgat tccaatcagt
      601 ttactaaaaa attggaatga acagacagga aaaaatttga caactaatga tcttattggc
      661 aaatcagtct cagccagcat tgtagaaagt gctgccgaaa ctagtaagat tgctcaattt
      721 caaacgaaga ttgtacgtgt aatcaatgat gaagatgaca tggaggacag caacagtttc
      781 atgctgtctc atcaaatgga aacgattttg aaagaggctg gatttacgaa agctgtatct
      841 tattttatct tggaactcaa agatccatca cagacaaaag tagtaacaga agaattacag
      901 aaaaataaga agtatactgt gctttctcaa cagagagttc ttgatattgt gattaccttt
      961 attcgtgtta ttcagggatt attgattgtg ctttcatcac aagctattgt ggtagcagcg
     1021 gttatgattg gtatcattat ttacatcaat atcatgcaac gttccaagga aataggtgtc
     1081 atgaaagcag ttggttatca gaatcgtggt gtcaaaggaa tttttattta cgaggctatc
     1141 tggattgtag gcatcgcctt gctgctggca tttttggttg cacaaggggt gggaagtttg
     1201 gcgaatgcga ttgtaagtca cttttaccca tccatcacta aggtttttga attaaatctt
     1261 ttatctgttt taggaactct agttttcgct ctattacttg gttatgtctc agcctacttc
     1321 ccggcgcgta agattagtaa aatggatcct gtagaatcgt tacgctatga ataataaggg
     1381 attccttgat ttacagtctt aaaaagaaac tcacatagaa aaacaatggc catacttgca
     1441 attcaactca aatagttgta taataggttt gttgaaaggt gatttgtagt gattcaagtt
     1501 actcttttca caataaaaat ttcatgattt tcataaggag gaaatcacta atggtagtta
     1561 aagttggtat taacggtttc ggacgtatcg gtcgtcttgc tttccgtcgt atccaaaacg
     1621 tagaaggtgt tgaagttaca cgcatcaacg accttacaga tccagttatg cttgcacact
     1681 tgttgaaata cgacacaact caaggtcgtt tcgacggtac tgttgaagtt aaagaaggtg
     1741 gattcgaagt taacggtaaa ttcgttaaag tttctgctga acgtgatcca gagcaaatcg
     1801 actgggctac tgacggtgta gaaatcgttc ttgaagctac tggtttcttt gctaagaaag
     1861 aagcagctga aaaacacctt aaaggtggag ctaaaaaagt tgttatcact gctcctggtg
     1921 gaaacgacgt taaaacagtt gtattcaaca ctaaccacga cgttcttgac ggtactgaaa
     1981 cagttatctc aggtgcttca tgtactacaa actgcttggc tccaatggct aaagctcttc
     2041 aagataactt tggtgttgtt gaaggattga tgactactat ccacgcttac actggtgacc
     2101 aaatgatcct tgacggacca caccgtggtg gtgaccttcg ccgtgctcgc gctggtgctg
     2161 caaacatcgt tcctaactca actggtgctg ctaaagctat cggtcttgta atcccagaat
     2221 tgaacggtaa acttgacgga tctgcacaac gcgttccaac tccaactgga tcagttactg
     2281 aattggtagc agttcttgaa aagaacgtta ctgttgatga agtgaacgca gctatgaaag
     2341 cagcttcaaa cgaatcatac ggttacacag aagatccaat cgtatcttca gatatcgtag
     2401 gtatgtctta cggttcattg tttgacgcaa ctcaaactaa agttcttgac gttgacggta
     2461 aacaattggt taaagttgta tcatggtacg acaacgaaat gtcatacact gcacaacttg
     2521 ttcgtactct tgaatacttc gcaaaaattg ctaaataatt cttgagttga tagaaagcaa
     2581 ggctttgtgg tcttgctttt tatatggaaa aatggatgac acgatcatcc attctttttt
     2641 aattcttttt caaatgtatt tgaaagggta gtgaaagtta gcttttctaa agtaagtggg
     2701 tgggtaaagg aaagtcggaa ggcatgaagc ataagccgac ttgtctttga tttactatta
     2761 tagagagggt cgcccaggat aggaagatta tgatgcgaaa ggtgcacacg aatctgatgg
     2821 gttcgccctg tctttagctt gcaacgagcc aaggaagtct tgtttgagaa ttgctttaat
     2881 ctgcttacat gcgtttcagc atattgccca ttttttgcat caactattct ttttctacga
     2941 tcatggcgat cacgtccaat tttgtctctg aaaacaagtt cttttctgtt gatatttcca
     3001 tcaactagag cccaatattc tctagaaatc tcttttttct ccaataagcg attgagaatg
     3061 ggcaggataa aaggattttt ggcaaagaga actaagccac tggtttccat gtccagacga
     3121 tgaacgacat agcaggtttg gccaacatag gtactgacat ggttaagaag ggcaatttcg
     3181 tttggttgat taccatgcgt tttcatcccc tctggtttgt ttacaataat caagtgttga
     3241 tcttgataaa cttcctgcac taagtctggg ttgccccaag ggatcgtctt ttgggaataa
     3301 tcttcctcgt caaaagtcaa ctggcaaaca tctccaggat ttacgatttc gttccagtgg
     3361 acttcttctt gatttatcaa aatatgtttc ttgattctca aaaaatgacg gatttttcta
     3421 gggatgagga gttgttcctc aagtaattgc tttaccgtca tttgaggtag agaggcgggt
     3481 aatgtaaatg tgaattgcat acagatattg taacaaaaaa agccctattt ggataggaaa
     3541 tagctaaatt cttgtcttcc tatgatgaag atgataaaat aaacgcatga aattagataa
     3601 attatttgag aaatttcttt ctctttttaa aaaagaaaca agtgaactag aggactctga
     3661 ttctactatc ttacgtcgct ctcgtagtga tcgaaaaaaa ttagcccaag taggtccgat
     3721 tcgaaaattc tggcgtcgtt atcatctaac aaagattatc cttatactag gtttgagtgc
     3781 aggattgcta gttggaatct atttgtttgc tgtagccaag tcgaccaatg tcaatgattt
     3841 gcaaaatgcc ttgaaaactc ggactcttat ttttgaccgt gaagaaaaag aggctggtgc
     3901 cttgtctggt caaaagggaa cctatgttga gctgactgac atcagtaaaa acttgcagaa
     3961 tgctgttatt gcgacagaag accgttcttt ctataaaaat gacgggatta actatggccg
     4021 tttcttcttg gctattgtca ctgctggacg ttcaggtggt ggctctacca ttacccaaca
     4081 gctggctaaa aacgcctatt tatcgcagga tcaaactgtt gagagaaaag cgaaagaatt
     4141 tttccttgcc ttagaattaa gcaaaaaata tagtaaggag caaattctaa ccatgtacct
     4201 taacaacgct tattttggaa atggtgtgtg gggtgtagaa gatgcgagta agaaatactt
     4261 tggagtttct gcatcagaag tgagtctgga tcaagctgcg actctggcag ggatgctcaa
     4321 ggggccggaa ctgtataatc ccttgaattc tgtagaagat tctactaatc ggcgcgatac
     4381 tgtcttgcag aatatggttg cagcaggata tattgataaa aaccaagaaa ccgaagctgc
     4441 tgaagttgat atgacttcgc aattgcacga taagtatgaa ggaaaaatct cagattaccg
     4501 ttacccctct tattttgatg cggtggttaa tgaagctgtt tccaagtata atctaacaga
     4561 ggaagagatt gtcaataatg gctaccgcat ttacacagag ctggaccaaa actaccaagc
     4621 aaatatgcag attgtttatg aaaacatatc gctatttccg agggcagagg atggaacgtt
     4681 tgctcaatca ggaagtgtag ctctcgaacc gaaaacaggg ggagttcgtg gagttgtcgg
     4741 tcaagttgct gacaatgata aaactggatt ccggaatttc aactatgcaa cccaatcaaa
     4801 gcgtagtcct ggttctacaa ttaagccttt agttgtttat acgccagcag ttgaagcagg
     4861 ctgggctttg aataagcagt tggataacca taccatgcag tatgacagct ataaggttga
     4921 taactatgca gggatcaaaa cgagtcgaga agttcctatg tatcaagcct tggcagaatc
     4981 gcttaatcta cctgctgttg tcactgttaa tgatttgggt gtcgacaagg cttttgaggc
     5041 aggcgaaaaa ttcggactca acatggaaaa ggttgaccgt gttcttggtg tcgccttggg
     5101 aagcggtgtt gaaaccaacc ctcttcaaat ggctcaagca tacgccgcct ttgcaaatga
     5161 aggtttaatg cctgaagctc attttattag tagaattgaa aatgctagtg gacaagttat
     5221 tgcgagtcat aaaaattcac aaaaacgggt gattgataag tctgtagctg acaagatgac
     5281 cagtatgatg ttggggactt tcaccaacgg taccggtatt agttcatcgc ctgcagacta
     5341 tattatggca gggaaaactg gaacaactga agcagttttt aatccggagt acacaagtga
     5401 ccagtgggta attggttata ctccggatgt agtgattagc cactggcttg gctttccgac
     5461 cactgatgaa aaccactatc tagctggctc tacttcaaac ggtgcagctc atgtctttag
     5521 aaacattgcc aataccattt taccttatac gtcaggaagt acctttacgg ttgaaaatgc
     5581 ttataagcaa aatggaattg caccagccaa tacaaaaaga caagtacaaa ccaatgataa
     5641 tagccagaca gatgataatt tgtctgatat tcgagggcgt gcgcaaagtc tagtagatga
     5701 ggctagccgg gctatctcag atgcgaagat taaggaaaag gctcaaacaa tatgggattc
     5761 gatagtcaat ctatttcgct aagatgcttg tcaaagccta gctttcttgt tataatagat
     5821 aggatggagg cgttatggca ctaaaaaaag caagcctagc ttgtgcggtt tgtggttcga
     5881 gaaactattc aatcaagatc agcggaaacc ccaagcctac acgactagaa gtaaataaat
     5941 tttgtaagca ttgtggcaag tacactacac atagagaaac gagataggag agagcgatgc
     6001 gttttattgg agatattttt agacttctta aagacacaac gtggccaact cgcaaggaaa
     6061 gctggagaga ttttcgttct atcatggaat acacagcttt ctttgtagta attatttaca
     6121 tttttgacca gttgattgtt tcaggtttga ttcgatttat taacattttt tagaagatta
     6181 gtggagttaa ttacactaga aatcttctat ttatgaaagg aaatatcatg gatagttttg
     6241 ataaaggatg gtttgtttta caaacttatt ctggttatga aaataaggtg aaagaaaatc
     6301 tattacaacg tgcacaaacc tacaatatgt tggataatat tctacgcgtt gaaattccaa
     6361 cacaaacagt gcaagttgaa aaaaatggaa agagaaaaga agtagaagaa aatcgctttc
     6421 caggttatgt tcttgtagaa atggtcatga cagatgaagc ttggtttgtt gttcgaaaca
     6481 caccaaatgt tacaggattt gtcggatcac acgggaacag atcaaaacca actccattat
     6541 tggaacaaga aattcgtgac attttggtat ctatgggaca aactgttcaa gaatttgatt
     6601 tcgatgttga gattggtcaa actgtacgta ttattgatgg tgcttttgca gactacactg
     6661 gtaagattac agaaattgat aataacaaag tgaaaatgat tatctctatg tttggtaatg
     6721 acacagttgc agaagtaaac ctaaaccaaa tcgcagaat
//
LOCUS       DBERMR010000079         6318 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.079, whole genome shotgun sequence.
ACCESSION   DBERMR010000079 DBERMR010000000
VERSION     DBERMR010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6318
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.079"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            104..1483
                     /gene="glmU"
                     /locus_tag="ACL8A1_002030"
     CDS             104..1483
                     /gene="glmU"
                     /locus_tag="ACL8A1_002030"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002899862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HGS1202734.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            1493..2038
                     /locus_tag="ACL8A1_002031"
     CDS             1493..2038
                     /locus_tag="ACL8A1_002031"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HGS1202735.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELKKK"
     gene            2050..2364
                     /gene="macP"
                     /locus_tag="ACL8A1_002032"
     CDS             2050..2364
                     /gene="macP"
                     /locus_tag="ACL8A1_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HGS1202736.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKNHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            2381..3073
                     /locus_tag="ACL8A1_002033"
     CDS             2381..3073
                     /locus_tag="ACL8A1_002033"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HGS1202737.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNAQEQVVFGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQKSLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            3150..3641
                     /gene="rocS"
                     /locus_tag="ACL8A1_002034"
     CDS             3150..3641
                     /gene="rocS"
                     /locus_tag="ACL8A1_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HGS1202738.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            3739..4329
                     /locus_tag="ACL8A1_002035"
     CDS             3739..4329
                     /locus_tag="ACL8A1_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HGS1202739.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            4338..6101
                     /locus_tag="ACL8A1_002036"
     CDS             4338..6101
                     /locus_tag="ACL8A1_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HGS1202740.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDRYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAMLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         1954 a         1063 c         1381 g         1920 t
ORIGIN      
        1 aatgggttaa ggaagagact ttagagcatt ttttcattca agagtgcgga atgatttgaa
       61 atatggtata ataaaaggga atttctacag aaaagagaag attatgtcaa attttgccat
      121 tattttagca gcgggtaaag ggactcgcat gaaatctgat ttgccaaaag ttttgcacaa
      181 ggttgcgggt atttctatgt tggaacatgt tttccgtagt gtgggagcta tccaacctga
      241 aaagacagta acagttgtag gacacaaggc agaattggtt gaggaggtct tggctggaca
      301 gacagaattt gtgactcaat ctgaacagtt gggaactggt catgcagtta tgatgacaga
      361 gcctatctta gaaggtttgt caggacacac cttggtcatt gcaggagata ctcctttaat
      421 cactggtgaa agcttgaaaa acttgattga tttccatatc aatcataaaa atgtggccac
      481 tatcttgact gctgaaacgg ataatccttt tggctatgga cgaattgttc gtaatgacaa
      541 tgctgaggtt cttcgtattg ttgagcagaa ggatgctaca gattttgaaa agcaaatcaa
      601 ggaaatcaac actggaacat acgtctttga caacgagcgt ttgtttgagg ctttgaaaaa
      661 tatcaatacc aataacgctc aaggcgaata ctatattaca gacgtcattg gtattttccg
      721 tgaaactggt gaaaaagttg gcgcttatac tttgaaagat tttgatgaaa gtcttggggt
      781 aaatgaccgt gtggcgcttg cgacagctga gtcagttatg cgtcgtcgca tcaatcataa
      841 acacatggtc aacggtgtta gctttgtcaa tccagaagca acttatatcg atattgatgt
      901 tgagattgct ccggaagttc aaatcgaagc caatgttacc ttgaaagggc aaacgaaaat
      961 tggtgctgag actgttttga caaacggtac ttatgtagtg gacagcacta tcggagcagg
     1021 agcggtcatt accaattcta tgattgagga aagtagtgtt gcagacggtg tgacagtcgg
     1081 tccttatgct cacattcgtc caaattcaag tctgggtgcc caagttcata ttggtaactt
     1141 tgttgaggtg aaaggatctt caatcggtga gaataccaag gctggtcatt tgacttatat
     1201 cggaaactgt gaagtgggaa gcaacgttaa tttcggtgct ggaactatta cagtcaatta
     1261 tgacggcaaa aacaaataca agacagtcat tggagacaat gtctttgttg gttcaaattc
     1321 aaccattatt gcaccagtag aacttggtga caattccctc gttggtgctg gttcaactat
     1381 tactaaagac gtgccagcag atgctattgc tattggtcgc ggtcgtcaga tcaataaaga
     1441 cgaatatgca acacgtcttc ctcatcatcc taagaaccag taggagccta tcatggagtt
     1501 tgaagaaaaa acgcttagcc gaaaagaaat ctatcaagga ccaatattta aactggtcca
     1561 agatcaggtt gaattaccag aaggcaaggg aactgcccaa cgggatttga ttttccacaa
     1621 tggggctgtc tgtgttttag cagtaacgga tgaacaaaaa cttatcttgg tcaagcagta
     1681 ccgcaaagct atcgaggctg tctcttacga aattccagcc ggaaaattgg aagtaggaga
     1741 aaacacagcc cctgtggcag ctgcccttcg tgaattagag gaagaaacag cctatacagg
     1801 gaaattagaa ctcttgtacg atttttattc agctattggc ttttgtaatg agaagttaaa
     1861 actatattta gcaagcgatt tgacaaaagt ggaaaatccg cgtccgcagg atgaggatga
     1921 aaccttggaa gtccttgaag tgagcttaga agaagcgaaa gaattaatcc aatcaggtca
     1981 tatttgtgat gccaagacaa ttatggctgt tcagtattgg gagttgaaga aaaaatagag
     2041 gaggtcagta tgggtaaatc tttattaacg gatgaaatga ttgaaagagc taatagaggc
     2101 gaaaaaattt caggtcctcc tttgctagat gataatgagg agactaagat tttaccaacc
     2161 tcttcttccc gttttggtta tgccaatcct aagaatcatg gttttagcca ggaaaccttg
     2221 aagattcagg tcgaaccatc tattcataaa agccgtcgca ttgaaaatac caagagaaat
     2281 gtcttcaatt ctaagttgaa taaaatctta ttcgcggtca tctttctctt gattttgctt
     2341 gttttagcaa tgaaactttt gtaatagaaa aggaattgaa atgaaaatag gaattattgc
     2401 tgctatgcca gaagaactgg cttatctggt ccagcattta gataatgccc aggagcaagt
     2461 tgtttttggg aatacctatc atacaggaac cattgcttct catgaagtcg ttcttgtaga
     2521 aagtggaatt ggtaaggtca tgtctgctat gagtgtggcg attttggctg atcatttcca
     2581 ggtggatgcc cttattaata cgggttcagc tggggcagta gcagaaggta tcgctgttgg
     2641 ggatgtcgtg attgctgaca aattagccta tcatgacgtg gatgtcacag cttttggcta
     2701 tgcttatgga caaatggcgc aacaaccgct ttatttcgaa tcagacaaaa cctttgttgc
     2761 tcaaatccaa aagagtttat ctcaattgga ccaaaactgg catcttggtt tgattgctac
     2821 aggagatagt tttgttgcag gaaatgacaa gatagaagcg attaagtccc atttcccaga
     2881 agttttagcc gtggagatgg agggggcagc tattgctcaa gcagcgcatg ccctcaatct
     2941 cccagtctta gtcatccgag ctatgagtga caatgccaac catgaagcaa acatcttttt
     3001 tgatgagttt attatcgaag ctggacgtcg ctctgcccaa gtcttgttga cctttttgaa
     3061 ggctttagat taagcggaaa tttgacagtt tttctagctt atgataagat ttaagtaaag
     3121 aaaagctaga aaacgtttca gaggatatta tgagtattga aatgaccgtc agtgagattg
     3181 cagaggtctt aggattatct cgccaagcaa tcaataaccg tgtcaaagaa ttaccagaag
     3241 aagacacaga taaaaatgac aaaggggtaa cagttgttac cagaagtggc ttgattaagc
     3301 tagaagaaat ctataaaaaa acgatttttg aagatgaacc tgtcagtgaa gatgtcaaac
     3361 aacgtgaact gatggagatt ctagtggatg agaagaatgc agaaattctt cgtctttatg
     3421 aacaattaaa agccaaggat cgtcagttat cagaaaaaga cgagcagatg cgtatcaaag
     3481 accgtcagat tgctgagaaa gacaaacaat tagatcagca acaacaattg acccttcaag
     3541 ccatgaagga tcaagaaaat cttaagctag agctggacca agcaaaagaa gaagtccaat
     3601 ccactaaaaa aggctttttt gctcgtttat ttggaggata atcaaggagc tgtttaggtt
     3661 aaatgctaac ctgatggctt cttttgtttg tgaatagtat agttgattag aacagagaca
     3721 gtagaggaga ggtacagaat ggaacgatta gaagattaca ttgcttttga tttagaattt
     3781 aatcagcacg aaggacaaac acacttgatt caagtatcgg cagtgcgttt tagagatggt
     3841 cgggaaatcg atgcctatga ttcctatgtt cataccagtg tccctctaaa aagctttatc
     3901 aatggtttga ccgggattac agctgagacc ttaaaagacg ctcctccagt tgagaaagtc
     3961 ttagaagaat tccaagagtt tgtgggttct ttgctgatga taggctacaa tgctgctaaa
     4021 agtgatttac ctattttagc agagcatggt ttagactata gccaacagta caaggtggat
     4081 ttgtatgatg aagcttttga acgtcggagt tcagatttac atggtattgc caatctcaaa
     4141 ttgcaaactg ttgcctcatt tcttggattt caaggtcagt ctcataatag tttagaggat
     4201 gctcggatga cggcgcgtgt ttacgaatcc ttcttagagt cggatcaagc tagggaattg
     4261 ttagagacag aaagtagctt atcaaacaat ccttttggtg gcttggactt gtctcaatta
     4321 tttgactagg agtgcctatg aaacctgaaa aacctaaaaa attgggtctt aaacagattt
     4381 acctcaatct tgataaaatc ctctttctat ttttcttgat ttttatgata gtggagtatt
     4441 tgtgggtacc attcaattca tggttggcta gcttcttatt agatcagaca ggttatcttt
     4501 ttatttccta taacaatgtc ttagccatcc ttactcattc tccattgatt agtctagctt
     4561 ttttagcctt gattgtaatt aaccttctgg ttgcttattt tcaaatagga ctcctcttta
     4621 ttggtgctcg tcatctcctc tataatgaaa agcgaaggct aattgagtat atccgcaagg
     4681 ttttccatga aagttttgga tttatgaaaa agctaacttg cgctaaagcc ttgtttatct
     4741 ttttctatat agctatgctg tttcctttta tacggaaaat gctgaagatt tattatctta
     4801 ataaaatcgt cattccagag tttatccaag cttatttaga agatagatat tggatgtggt
     4861 ggctgagcat cctacttcta tctcttatct ttctctatgt ctctgtcaga ttgatgtttg
     4921 cccttccgaa gattgtatat gatcagctga ctgttcgaga agcagtgatg tttagcttgg
     4981 agaaaaccaa gaagagagtt actttttatg cttggaattt attttacatc ttgctcaaag
     5041 cacatttatt attttatctt cctttgattc ctttattatt ggctcaaact ttggtagatg
     5101 atatcactca gagagagtct ctgattcttg gtattttgaa ttttgtcgtg attaaaaatc
     5161 tctattatat ggctctgact tattttttgg ttaaatttgt ttcatttttg acaggaaagg
     5221 agctggatat gcttcctagg agagaaaaag atcacatcgt gcgatggggt gtcatgactt
     5281 gtgccagtct tttctttgcc ttagaaggtt atatttatct ggaggctccc atggttcatc
     5341 tacctcaact tatttctcac cgaggggttt ccaatgcaaa tgggattcaa aatacagtag
     5401 agtccttgga aactacagca caactcaaac cagacttggt ggagacggac gtgcaggaaa
     5461 caaaagatgg gcagtttgtc atgatgcatg atgctaactt gaaaaatcta gcaggtatca
     5521 ataaaagtcc tcaagactta aacttggagg agcttaaagg gattgatatt tttgaaaatg
     5581 gctaccagac taaaatttca agctttgaag attatctcag tcgagccaac gaacttggtc
     5641 aaaaattact aattgaaatt aaaaccagta aaaaagatag tccagacatg atgaaccgct
     5701 ttttagcccg ttatgctgca atgctcaaga tttatggaca tcaaatccag tctttagact
     5761 accatgttgt cgaaaaagta agacagtatg atgccgaact gccagtttat ttcatcatgc
     5821 cctacaattc tgtctttcct aaaacaagag cgacaggata tactatggag tactcaacct
     5881 tggatgaata ttttgtaagc aaactatgga caacggatca gaaactttat gtctggactg
     5941 tcaatgattc agaagctatc agcaaatctc ttcacttagg agtagatgga gtgattacag
     6001 atgatttgga aaaagttcag cgggaaatag aagtagccca agaagatcca gaatatacgg
     6061 atttgctctt gaaaaaagca ttggaattct ttgaatttta gtaataaaaa agaggaacgg
     6121 ttgttcctcc catgataggt aagtattctt gtgaatgctt gcttttttgt ggttgcaatc
     6181 cgtttacgag aattgtttat attgttcttg tttcaattta ctttatagtg ctttgaagtt
     6241 ggaatgctac actataactt ataaaatata gtaaaatgaa ccaaaaatag tacacaatgt
     6301 ggtataatct tcttatgg
//
LOCUS       DBERMR010000080         6238 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.080, whole genome shotgun sequence.
ACCESSION   DBERMR010000080 DBERMR010000000
VERSION     DBERMR010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6238
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.080"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            164..850
                     /locus_tag="ACL8A1_002037"
     CDS             164..850
                     /locus_tag="ACL8A1_002037"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HGS1202741.1"
                     /translation="METKKIKNLKGQIIVSCQALEGEPLYTPNGGVMPLLAKAAFQAG
                     AKGIRANSVRDISEIKEEVDLPIIGIIKRDYDGFEPFISATMKEIDELVSEGVDILAL
                     DCTNRSRPGYDNITDFIHDIKVKYPNQLLMADISTFEEGKVAAESGVDFVGTTLSGYT
                     PYSPKKDNPDFELVERLVKELDVPVIAEGRISTPEQARKMLDLGAYAVVVGGAITRPL
                     EIAKKFIEVV"
     gene            851..1783
                     /locus_tag="ACL8A1_002038"
     CDS             851..1783
                     /locus_tag="ACL8A1_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1202742.1"
                     /translation="MNQEILKKSKIQLTVEHIQKYWILYLMMIPATVLLILFTYGPMY
                     GIIMAFQDFTVFKGYTGSSFVGLKHFQRLFSDPLFYRLFKNTFMVGVLDFLFSFPAPL
                     IFALILNEVRKVRFKSVVQSISYLPHFIPLVVMVGIIFELFGSYGIINSLLSSLGMEP
                     ISFFTKSEWFLPLYIGSGVWKTIGWGSIIYMGALTNIDSTLYEAADMDGANRWHKMWH
                     VTLPSLRPTVVTLFILNAGGIMQVGFEKVFLMSSPATYEVSDVLSTYVYRQGILNSDF
                     SYSAAVGLFNNIVALLFVLLANKIAKKLGEEGIV"
     gene            1794..2723
                     /locus_tag="ACL8A1_002039"
     CDS             1794..2723
                     /locus_tag="ACL8A1_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS1202743.1"
                     /translation="MIFSKNTKKSNRDKETFVDYLFIFILIIITLLMLYPLWWVVMTS
                     LSEPVWLSKNVVKLFPLKIDITAYKFIFGDSKLWQAYGNSVLYAALSTIVSVAVCSMY
                     AYPLTIPEIRGKKFWNTLILIPMFFSGGMIPLYIQVANLGLLNTAWALVLPGAVSTYY
                     IILFRTQFKSIPMELRESARIDGASEFRIFWNLVLPLSKVIFMIIALYCIVGSWNSFT
                     PPLLYLNDDTKMPLSIYLRNLVVTNTTDTAELSLSAGNTYYQQIAAQGGGIGMRIAIK
                     MGTIVVSVIPIMMIYPFIQKHFVKGILLGSVKG"
     gene            2737..4176
                     /locus_tag="ACL8A1_002040"
     CDS             2737..4176
                     /locus_tag="ACL8A1_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202744.1"
                     /translation="MQNLKKYLLLDSGLVVGYTLENAQLKLNKLKKDTENNPLFGEEY
                     FAENPKLWEVRFDNSYPNVIYDKELKLYRCWYQTFVSDEASEETPLAERGEKEYIVKS
                     SRMTALCYAESKDGVKWEKPNLNLVKFKGSKDNNIVLMHAHGAGIMLDEQELDPKKRY
                     KLVSKLEFSPNLHYMVVVFSEDGIHFDEPIPWKKWNPAADSHNFPFRDPKTGKYAVIT
                     RTWANNVRLSAITFSDDFINWSEPKEILRGDGFEDQIYSMPVISYADIYLGFASMYHE
                     GDTLNENYDKVDLELKFSANLEQWDSVCKGDYLIERGGGVYPQNADFDASCIFTAAPI
                     IEDDKIWFYYMGGNGNHTGFRESSLARGYIKKDQFAYYTNKREGIPAKISTVSFQTYD
                     DEIYILADIPENGYIKVAVGTKYGNAYSDFGQEDISLVQVSDGKYKLEFNKKSILDLG
                     NSPVAFKFEIENAKLYGIEGSIQQHYLKY"
     gene            4245..5822
                     /locus_tag="ACL8A1_002041"
     CDS             4245..5822
                     /locus_tag="ACL8A1_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGS1202745.1"
                     /translation="MVKLNKVLKGSVAFLSASLLLAACGNNANSKPAEKTADGKTKIS
                     FLSTWQINDVRPPKDETLVGQWLEEQLGIDIDLVTIPGEGVSSKVNSLITSNQLPTVL
                     LTTGDKSDIAGINKLGKQGAFLDLSQHMDKLPNYKKYLEKNNALAQIEDDEKHIYAFT
                     KFFTDENIMYTTPILRKDLLVGSEFEDLSKIKTVDDYTKVLKYLTEKQGSPAFIQRNG
                     YEGFMKRVTPLWNLSHRTYYDYESDSYKHPVEQPQLKQFVEWLKELRKDNVLHPDWAV
                     MKDETWEGLLASNKGSFTVDRMNIIGDNNFDKSFDWQPLVYPKINGKRYLQPKTPYIS
                     DSGWVINANAPKEEIDKALEFFNFLYADENQEKLAIGFEGKTFTRENPNTQAGIKWLI
                     QIYGENAGNDKAELLFKHGNQAFTRVQTKLDIEAQPGTFPKVMYDQVDKIKKDLGGFR
                     PDTPVLSFTTEELDVLTKSEAGLNTFFDENIIKFIEGQRDMSEWDSFINEAKSKDLDK
                     VTKVYNDAYKRVKDRMK"
     gene            5882..>6238
                     /locus_tag="ACL8A1_002042"
     CDS             5882..>6238
                     /locus_tag="ACL8A1_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGS1202746.1"
                     /translation="MYDVVIIGAGSSGALAAIASGRLGLKTCLIEKGSRIGGVPINTL
                     MGSFANLYFDDSGRVNASKIVGELIERIIKKGGTVFNSMEELMNVNDFYQITIPYQPE
                     VYESVLTDMLIESKVDI"
BASE COUNT         2115 a          856 c         1196 g         2071 t
ORIGIN      
        1 attgaatcta taacagtacg ccttaaatgc taaaatattt ctataaatta atttaacttt
       61 cctgataggg ctgttcatat cttatttcaa ttcactatat aaataaaatt gtagtagatg
      121 ttattgaatt agtaaaaaaa ataaaaaaac ggaggttttc tgaatggaaa ctaaaaaaat
      181 taaaaattta aaaggtcaaa taattgtttc ctgccaagcg ttagaaggag agccacttta
      241 tactccaaat ggaggagtga tgccgttatt agcaaaagcc gcctttcaag caggcgctaa
      301 aggtattcgt gctaattctg tccgcgatat ttcagaaata aaagaagaag ttgacttacc
      361 aattataggt atcataaaaa gagattacga tggctttgaa ccttttattt cagcaactat
      421 gaaagagatt gatgaattag ttagtgaagg agtagacatt ttagctttgg attgtactaa
      481 tcgttcaaga ccaggttatg ataatattac tgactttatt catgatatta aagtcaagta
      541 tccaaaccaa ttattaatgg ctgatatttc tacttttgaa gaagggaaag tagctgctga
      601 atcgggagta gattttgtgg gtacgacatt atctggttat acaccatata gccctaaaaa
      661 agataatcct gattttgagt tagttgaaag attggtaaaa gagcttgacg taccagttat
      721 tgctgaagga agaataagta ctccggaaca agctagaaaa atgttagatt taggtgctta
      781 cgcagtggtt gttggaggag ctatcactcg tcctttggaa atagcgaaga agtttattga
      841 ggtagtttag atgaatcaag aaatattaaa aaagagtaag attcaattga cagttgaaca
      901 tattcaaaaa tattggattt tatatttaat gatgattcca gcaactgttc tactaatttt
      961 atttacgtat ggaccaatgt acggcattat tatggccttt caagacttta ctgtcttcaa
     1021 gggatatact ggaagttcat ttgtcggttt aaaacatttt caaagattgt tctcagatcc
     1081 tttgttttat agattgttca agaatacgtt catggtaggg gttttagact ttttattctc
     1141 tttcccagct ccgcttattt ttgcactcat attaaacgaa gtcagaaaag taagatttaa
     1201 gagtgtagtt caatctatta gttatcttcc tcattttatt cccctagtgg ttatggttgg
     1261 tattattttt gaactatttg gttcatatgg tattattaac agtttacttt catcacttgg
     1321 aatggagcca ataagtttct ttacaaaatc tgaatggttc ttgcctctct acattggttc
     1381 aggagtatgg aaaacaatag gttggggttc tatcatttat atgggagcat taaccaatat
     1441 agattcaact ttatatgaag cagcagatat ggacggggct aatagatggc acaaaatgtg
     1501 gcatgtaact ctaccaagtt taagacctac agtagttact ttgtttatac taaatgctgg
     1561 tggtatcatg caagtaggat ttgaaaaggt attcttgatg tctagtcctg caacatatga
     1621 agtatctgat gtattatcta cttatgttta tagacaaggt attttaaatt ctgatttcag
     1681 ttactcagct gcagtaggat tatttaataa catagtagct ttgttatttg ttttattagc
     1741 taataagatt gctaaaaaac ttggcgaaga aggaattgta taaggggggt aaaatgattt
     1801 tttcaaaaaa tacaaaaaag agtaatagag acaaagaaac cttcgttgat tatctcttta
     1861 tattcatttt aataataata accttactta tgttgtatcc attatggtgg gtagtgatga
     1921 cgtcactttc agaaccagtt tggctatcta aaaacgttgt taaattattt cccttaaaaa
     1981 tagatattac tgcatataag tttatttttg gggattctaa actatggcaa gcatatggta
     2041 atagtgtatt gtatgctgca ctatctacaa ttgttagtgt tgcggtatgt agtatgtatg
     2101 cctatccact tacaattcct gaaattaggg gtaaaaaatt ctggaatact cttattttga
     2161 ttccaatgtt ctttagtgga ggaatgatcc cgctttatat acaagttgca aacctcggtt
     2221 tattgaatac tgcttgggct cttgttttac ctggtgctgt ttctacatat tatataattt
     2281 tgtttagaac acagtttaaa tcaatcccaa tggagcttcg tgagtctgct agaattgatg
     2341 gggcaagtga atttagaatt ttttggaatt tagtattacc attatcaaaa gttattttca
     2401 tgattatagc actttattgc atagttggtt catggaatag ttttactcct ccattacttt
     2461 atttgaatga cgacactaag atgcctttat ctatttattt gagaaattta gttgttacaa
     2521 atacaactga tactgctgaa ttatcacttt ctgcaggtaa tacatattac caacaaattg
     2581 ctgctcaagg tggaggtatt ggtatgcgta ttgctattaa gatgggtact attgttgtta
     2641 gtgttattcc aattatgatg atttatccat ttattcagaa acattttgtt aagggaatcc
     2701 tgctaggttc tgtgaaaggt taataaggta attattttgc aaaatttaaa aaaatatctt
     2761 ttattagata gcggtttagt agttgggtat acattagaaa atgctcaatt aaaattaaat
     2821 aagctaaaaa aagatactga aaacaatcca ctatttggtg aagagtattt tgccgaaaat
     2881 cctaaattat gggaagttcg ttttgataat tcatatccaa acgttatcta tgataaagaa
     2941 ttgaaattat atagatgttg gtatcaaaca tttgttagtg atgaagcaag cgaagagact
     3001 cctttagcag aacgtggaga gaaggaatac attgtcaagt catctagaat gacagcactt
     3061 tgctatgctg aaagtaaaga tggagtaaaa tgggaaaaac caaatctcaa tctagtaaaa
     3121 tttaaaggtt caaaagataa taatattgtt ctgatgcatg cacacggtgc aggtatcatg
     3181 ctagatgaac aggagttgga tcctaagaaa cgatataaat tagtttcaaa attagaattt
     3241 tctccgaatc ttcattacat ggttgttgtt ttttcagaag acggtattca ttttgacgaa
     3301 cctattccat ggaaaaaatg gaatcctgca gcagatagtc ataattttcc atttagagat
     3361 ccaaaaactg gtaaatacgc tgttattaca agaacctggg caaataacgt acgtctaagt
     3421 gcaattactt tcagtgatga ttttattaat tggtcagaac ctaaagaaat ccttagggga
     3481 gatggatttg aagatcaaat ttattctatg ccagtaattt catacgcgga tatctattta
     3541 ggttttgctt caatgtacca cgaaggcgac actttaaatg aaaactatga taaagtagac
     3601 ttagaattga aattctctgc taatttggaa caatgggata gtgtatgtaa gggcgactat
     3661 ctcattgaaa ggggaggtgg agtatacccg cagaatgctg attttgatgc tagttgtatt
     3721 ttcacggctg ctccaataat tgaagatgat aagatttggt tctactatat gggtggaaat
     3781 ggtaatcaca ctggcttccg agaaagttca ttagctagag gatatattaa gaaagaccaa
     3841 tttgcttatt atactaataa gagagaagga attccagcta aaatatctac agttagcttc
     3901 caaacttacg atgatgaaat ttatatctta gctgatattc ctgaaaatgg ttatattaaa
     3961 gttgcggtag gtactaaata cggtaatgct tattcagatt ttggtcagga agatatctct
     4021 cttgttcaag tttccgatgg taagtataag ttggaattta ataaaaaatc tatcttagat
     4081 ttaggtaatt ctccagttgc gttcaaattt gaaatcgaga atgcaaaatt gtatggtatt
     4141 gaaggtagta ttcagcaaca ctatctcaaa tattaaccag aataattcgt taacaaataa
     4201 tctctatatt agtggagata gatatataaa atggaggtct tattatggta aagcttaata
     4261 aggtattaaa agggagtgtt gcattcctga gtgcaagttt attgctagca gcttgtggca
     4321 ataacgctaa ttcaaagccc gctgaaaaaa cagctgatgg taaaacgaaa atttcgtttc
     4381 tatctacatg gcaaattaat gatgttcgtc cgcctaaaga tgaaacactt gttggtcaat
     4441 ggttagaaga acagctaggt attgatattg atcttgtaac tattcctggc gagggagtaa
     4501 gttctaaagt aaattctctt attactagta accagttacc gactgttttg ttgacaacag
     4561 gagataagag tgatatcgct ggtatcaaca aacttggtaa acagggtgca ttcttagatt
     4621 tgtcacaaca catggacaaa ctaccaaact ataagaagta ccttgaaaaa aataatgcat
     4681 tagctcaaat agaagatgat gaaaaacata tttatgcttt cacaaaattc tttactgatg
     4741 agaatatcat gtatactaca ccaattctga gaaaagactt acttgttggt tcagaatttg
     4801 aagatctttc aaaaattaaa actgttgatg actatacaaa agttttgaaa tatttgactg
     4861 aaaaacaagg ttctccagca tttattcaac gtaatggcta tgaaggcttc atgaagcgag
     4921 ttacaccatt gtggaattta agccatcgta catactatga ctatgaatca gattcatata
     4981 aacatccagt tgaacaacct caattgaaac aattcgttga atggttgaaa gaattacgta
     5041 aagataacgt acttcaccct gactgggcag ttatgaaaga tgaaacttgg gaaggtctat
     5101 tggcttctaa caagggttca ttcactgttg accgtatgaa tatcattgga gataataact
     5161 ttgataaatc atttgattgg cagccacttg tttatccaaa aattaatgga aaacgttatt
     5221 tgcaaccgaa aacaccgtac atttctgatt caggttgggt aatcaatgcc aatgcaccaa
     5281 aagaagaaat tgataaagcg ttagaattct ttaatttctt atacgctgat gaaaatcaag
     5341 aaaaacttgc tattggattt gaaggtaaaa catttacacg tgaaaaccct aatactcaag
     5401 caggtattaa atggttgatt caaatttacg gtgaaaatgc tggaaatgat aaagcagaac
     5461 tgctgtttaa acatggtaac caagcattta ctcgcgttca aactaaatta gatattgagg
     5521 cacagccagg aacattccct aaagttatgt atgatcaagt agataaaatt aagaaagatt
     5581 taggtggttt tagaccagat actccggttc tttcctttac aactgaagaa ttagatgttt
     5641 taactaaatc tgaagcagga ttaaatactt tctttgatga aaatattatt aaattcattg
     5701 aaggacaaag agatatgagt gagtgggatt catttatcaa tgaagctaaa tctaaagact
     5761 tggataaagt tacaaaagta tataatgatg cctacaaacg tgtcaaagat agaatgaaat
     5821 agactaaatg gtagctctct ggtttttcag agagctacta ctgcctgagg gggagtaagc
     5881 tttgtatgat gtagtaatta taggtgcagg aagttcgggc gcattggctg caattgcttc
     5941 ggggcgtcta gggttaaaaa cttgtctaat agagaaggga agtagaattg gtggagttcc
     6001 tattaataca ctaatggggt catttgctaa tctatatttt gatgattctg gtagagtaaa
     6061 tgcaagtaaa attgttgggg aattaataga acgtattatc aaaaaaggtg gtactgtctt
     6121 taactctatg gaagaattga tgaatgtaaa tgatttttat cagattacaa ttccctatca
     6181 accagaagtt tatgaatcgg ttttaactga tatgttgatt gagtctaaag ttgatatt
//
LOCUS       DBERMR010000081         6008 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.081, whole genome shotgun sequence.
ACCESSION   DBERMR010000081 DBERMR010000000
VERSION     DBERMR010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.081"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..208
                     /locus_tag="ACL8A1_002043"
     CDS             <1..208
                     /locus_tag="ACL8A1_002043"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202747.1"
                     /translation="LYYRLPIFAKLSEKKLEYMMLGDCVMLVNEMEITDHRVDNLFEK
                     GKNEIKDSCSRFSNRTYKSFRKSI"
     gene            153..437
                     /locus_tag="ACL8A1_002044"
                     /pseudo
     CDS             153..437
                     /locus_tag="ACL8A1_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
     gene            647..1306
                     /locus_tag="ACL8A1_002045"
     CDS             647..1306
                     /locus_tag="ACL8A1_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HGS1202748.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            1331..2398
                     /locus_tag="ACL8A1_002046"
     CDS             1331..2398
                     /locus_tag="ACL8A1_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGS1202749.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            2502..3512
                     /locus_tag="ACL8A1_002047"
     CDS             2502..3512
                     /locus_tag="ACL8A1_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1202750.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARVLVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            3524..5215
                     /locus_tag="ACL8A1_002048"
     CDS             3524..5215
                     /locus_tag="ACL8A1_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1202751.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            5218..5928
                     /locus_tag="ACL8A1_002049"
     CDS             5218..5928
                     /locus_tag="ACL8A1_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HGS1202752.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         2014 a          832 c         1150 g         2012 t
ORIGIN      
        1 actatattat cgcttaccaa tatttgctaa actttcagaa aaaaagttgg agtatatgat
       61 gctaggtgac tgcgtcatgt tagtaaacga aatggaaata actgatcata gagtagataa
      121 cttatttgaa aaagggaaaa atgagataaa agattcatgt agtagattta gcaataggac
      181 atataaaagc tttagaaaaa gtatctgaaa aaacagatgt ttatatctat aacctaggct
      241 cgggagaagg cactagtgta ttacaacttg taaatacatt tgaaagtgtt aataagatcc
      301 ctattcctta taaaatagtt ccaagacgtt caggagacgt tgcaacttgt tatgcaaatg
      361 cagacaaagc atataaggaa ttaaattgga ggacaacaaa atcgattgaa gacatgtgta
      421 gagatacatg gaattagcaa tcaaaaaatc ccaatggcta taattgatga tactgtctat
      481 atgagtgctg tattttggaa aaattactta accttttaat gaaatgcata tttaatgaaa
      541 gataaattaa aaaaaatgtt tgacagcgct tgacaaagga aacggtttca ttgtataatg
      601 taaacataat gacaccgatg tcaaatataa aaaagaaggt ctttatatga aaaacttaac
      661 aaaaattaag ttcaaagaga atggggaatt taatcatttc cctgggaata cagttgtagc
      721 aaatctttat actaaacaag atttgatgga agttgttgat attattcaat cacgttatag
      781 agaattacca tttatcgata agtttacttt aactccaagg aattctattc acatgactgt
      841 aattgaattg ttgtgccatg aaaatcgtga aacggagttt tggagcagta atcttcctct
      901 agatacacct ttacaggaaa tacatgatta ctttgctaaa caacttgaaa tttttccatt
      961 gttggatgaa gaaattcata tgcgtataac tgaaatggga aaacaaaaca tactagttga
     1021 acctgcagat gaagcttctg caaagagatt agaagaaatt cgtacttatg tctcagaaaa
     1081 agcaggtgtt tgtttcccta atcatgatag atatcaattc catatttcaa ttgggtatct
     1141 tcggattcct ctaaccgaag aggaggaaga agagtttact aaagtcagag cagaattaac
     1201 tgaaatttta ttagagaaga ttccaactat tactgtaaac cgtattgact atactgtatt
     1261 tgaagatatg agacaatttg ttccatatca cgaaaaattt aaataaaata ataaaaaagg
     1321 agaacttatc atgaaaaaaa tgaaagtttg gtctactgta cttgcaacgg gagttgctct
     1381 tactacactt gctgcttgct ctggaggttc aaattctacg actgcttctt catctgaaga
     1441 aaaagctgat aaaagtcaag aattagttat ctattcgaac tcagtctcaa atggtcgtgg
     1501 tgattggtta actgctaaag caaaagaagc tggttttaat ataaaaatgg ttgatatcgc
     1561 tggcgctcaa ttagcagacc gtgttattgc tgagaagaat aatgcagttg cagatatggt
     1621 atttggaatt ggtgctgttg actcaaataa aattagagat caaaaattac tagtacagta
     1681 caagcctaaa tggttagata aaattgatca atctttatca gataaagata attattataa
     1741 tcctgtgatt gttcaaccat tagttttaat tggggcgcct gatgtaaaag aaatgcctaa
     1801 agattggact gaattaggta gtaagtataa aggtaaatat tcaatttatg gtcttcaagg
     1861 tggtacagga cgggcaattc tagcaagtat cttagttcgt taccttgatg aaaaaggaga
     1921 tttaggcgta tcagaaaaag gatgggaagt tgcaaaagaa tactttgcaa atgcctatac
     1981 tcttcaaaaa ggtgaaagct cagttgttaa agcgttagat aaagaaagcc caattcaata
     2041 cggtatgatc tggggttctg gagcgttaat tggacaaaaa gaacaaaatg ttgaattcaa
     2101 agttatgact cctgagattg gtgtaccatt tgtaactgaa caaactatgg ttttaagcac
     2161 tagtaaaaaa caagcgttag ctaaagaatt tattgattgg tttggtcaat cagaaattca
     2221 agtagaatat agtaaaaaat ttggatctat tcctgcaaat aaagatgccc taaaagagtt
     2281 acctgaagat acgaaaaaat ttgttgatca agtgaaacca caaaatattg actgggaagc
     2341 tgttggaaag catttggatg aatgggtaga aaaagctgaa ttagaatacg taaaataaga
     2401 aataagagtt attttttatg tccaagtctc tctattattt ctaaatttaa ggaagaaata
     2461 aagagaggct tttggtttta gtaatagagg gagttagtga gatgattaaa tttgataata
     2521 ttcaaattaa atatggtgat tttgttgcaa ttgataatct gaatttagat atacatgaag
     2581 gggaattctt tacatttctt gggccttcag gatgtggtaa atcaactact ttgagagcat
     2641 tggtaggttt tttagatcca tcatcaggaa gtattgaagt taatggaaca gatgtcactc
     2701 atttggaacc tgaaaagcgt gggattggta ttgtatttca atcttatgcg ctatttccaa
     2761 ctatgactgt ttttgataat attgcatttg gtttaaaagt taagaaggta gctccagatg
     2821 ttattaaagc taaagtatca gcagtggcag caaaaattaa gatctctgat caacagttac
     2881 agcgtaatgt atcagaatta tctgggggtc aacaacaaag ggtagcattg gctcgtgttc
     2941 tggttcttga acctaaaatt ctttgtctag atgaaccatt gtcaaacctt gacgcaaaat
     3001 tacgtgtaga tttgagaaaa gagttgaaaa gacttcaaaa agagttaggt attactactt
     3061 tatatgttac tcatgatcaa gaggaagcct tgactttatc tgatagaatt gcagtcttta
     3121 acaatggata catcgaacag gtcggtacac cagtagagat ttatcataat tctcaaactg
     3181 aatttgtatg tgattttatt ggagatatta atgttttgac cgatgaaaca gtccacgaag
     3241 tattattgaa aaatacaagc gttttcttag aggataaaaa aggatacatt cgattagaga
     3301 aagttcgatt caatcgtgaa actgaacaag attttattct aaaagggaca attattgatg
     3361 ttgagttttc tggagttaca attcactata caataaaagt ttctgaaagt cagattctta
     3421 atgtaacaag tattgatagt caggctgcta ttagatctgt cggagaaagt gtggaattat
     3481 ttatcacacc atcagacgtt ctgcaatttt aaggaggtgc agtatgcgtc ataaattaaa
     3541 tttaaaagat tggcttattc gtttagggtt aatctggttc ttagtaacat ttattattta
     3601 tccaaacttt gatctagtag tgaatgtatt tgtaaaagga ggagaatttt cccttgatgc
     3661 tgtacatcgt gttctaaaat ctcagagggc acttcagagt attatgaaca gttttaagtt
     3721 agcattttca ctcattatta cagttaatgt cgtaggtatt ctttgtgttc tatttacaga
     3781 gtactttgat attaaaggtg ctaaaatttt aaaattaggt tatatgacct ctttaattta
     3841 tggaggagtg gttttagcga ctggatataa atttgtctat ggtccttatg gattgattac
     3901 aaaattttta caaaatgtta tcccttcttt agactctaac tggtttattg ggtatggtgc
     3961 agtcttattc attatgacat tttcaggaac tgctaatcat acattgtttt taacaaatac
     4021 aattcgaagc gttgactatc acactattga ggctgctcga aatatgggag caaaaccatt
     4081 tactgttttc cgaaaagtag tgttaccaac cttaattcca actctatttg cacttactat
     4141 tatggttttt cttagtggtt tatctgcagt agcagcaccc atgattgttg gtggtaaaga
     4201 atttcaaact ataaatccaa tgattattac atttgcaggg atggggaatt ctcgtgattt
     4261 agctgcctta cttgcaatta ttttaggtat tgcaactaca attttgctta ctatcatgaa
     4321 taagatagaa aaaggtggaa attatatttc tatctctaag actaaagcgc ctcttaaaaa
     4381 acaaaaaatt gcgtctaagc cttggaatat cattgctcac attgtagcat atggattgtt
     4441 cacagttttc atgcttccac taatttttat agtattatac tcatttacag atccagttgc
     4501 aattcaaaca ggtaacttaa cattatcaaa ctttacttta gaaaattatc gcttattctt
     4561 tagtaatagt gcggcattct ctccattctt ggtcagcttt atttattcta ttattgctgc
     4621 gacaacagca acaattctcg cagttgtatt tgctcgtgtt gtcagaaaac ataaatctcg
     4681 ttttgatttc ttatttgaat atggtgctct acttccttgg ttactaccaa gtacactttt
     4741 agcagtaagt ttattattta cttttaatca gccacaattt cttgtcttga atcagatttt
     4801 ggtaggtagt ttggtaattc tacttattgc atatatagtt gtaaaaatcc cattttctta
     4861 tagaatggta cgtgctattt tatttagtgt tgatgatgag atggaagatg cagcaagaag
     4921 tatgggtgct tcaccttttt atactatgat gaaggttatc attccattta ttttaccggt
     4981 tgttctctct gttattgctt taaactttaa ctctttatta actgacttcg acttatctgt
     5041 attcctttac catcccctag ctcaaccatt aggtattacg attcgatctg caggtgatga
     5101 aacagcaaca tctaatgcac aagctctggt atttgtttat acaattgttc tgatgattat
     5161 ttctggaacg gtattatact tcacacaaag accggggcgt aaagtaagga aataatcatg
     5221 acagccacta gtcttgggtt atcaaatatt gaaatagttg tcaggattgt tttatcagta
     5281 gtcattggta gtataattgg tttagagaga gggagcaaat cccagcctgc aggcatccga
     5341 acttatagta ttgtttgtct agctgcatgt ttgattatga tgacgaatga atacgtatct
     5401 tataaatttg ggacaggaga tcctacacga ttaggagctc aagttatatc aggtgtgggt
     5461 tttctaggcg ctggaacgat tcttattaca gataaaaaga aaattacagg tctgacaact
     5521 gcagcaggca tttgggcttc ggcaggaatt ggattagcta ttggagtagg tttttatgag
     5581 ggagctcttt tagtagccat ttctgtttgg ggtgtgatat ccatgttcca accactaaaa
     5641 aaatatctgc aaaatcgttc taaaatgatt gaattgtata tagtagttaa atctacagaa
     5701 gcctataatc gtgtattggt ttattgtgca gaaaatggta tcagaatgac cgattcaaga
     5761 acagcgtttg gagatgttaa ttctgacaga attgaatact ttgatgttcc ggacaaaaaa
     5821 atagcgtcat ttattactct ggaattatca ggtagatttg aacatcttcg actgatggaa
     5881 gaaattgcta atattgtagg tgttatttat gtcgaagaaa ttagctgaaa tttagtaaac
     5941 aaagggacag atgtttttgt ccttttgttt tttagatgtt ttaatcatct aatttttgag
     6001 tagatttt
//
LOCUS       DBERMR010000082         5827 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.082, whole genome shotgun sequence.
ACCESSION   DBERMR010000082 DBERMR010000000
VERSION     DBERMR010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5827
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.082"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            103..1128
                     /locus_tag="ACL8A1_002050"
     CDS             103..1128
                     /locus_tag="ACL8A1_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HGS1202753.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLG
                     NACKNAK"
     gene            1118..2125
                     /locus_tag="ACL8A1_002051"
     CDS             1118..2125
                     /locus_tag="ACL8A1_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HGS1202754.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     N"
     gene            2125..3132
                     /locus_tag="ACL8A1_002052"
     CDS             2125..3132
                     /locus_tag="ACL8A1_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HGS1202755.1"
                     /translation="MQNLIIGIQKRKNRITLFSSLFLLIIISLSFFILLIGDESYSFS
                     TLIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQTMLRNPLASPDIIG
                     VTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKDGFSAARLIILGIG
                     FQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLIVSIIATILVLFFN
                     KRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPIACISFLAGPIALN
                     IGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSPYLIYLLIKMNRRN
                     I"
     gene            3132..3926
                     /locus_tag="ACL8A1_002053"
     CDS             3132..3926
                     /locus_tag="ACL8A1_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1202756.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVSS"
     gene            4077..4261
                     /locus_tag="ACL8A1_002054"
                     /pseudo
     CDS             4077..4261
                     /locus_tag="ACL8A1_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612340.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            4380..4703
                     /locus_tag="ACL8A1_002055"
     CDS             4380..4703
                     /locus_tag="ACL8A1_002055"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGS1202757.1"
                     /translation="MLGYYEDIDDKNYRVFENFISVSFLGAVFYHKYKASLDMKIHCL
                     KLKNKELNKEVAFYLTSIIRQALKNTEYKDQISSTVLPDIKIKLPIDSRGTPDWNYME
                     RYRDR"
     gene            4684..4981
                     /locus_tag="ACL8A1_002056"
                     /pseudo
     CDS             4684..4981
                     /locus_tag="ACL8A1_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224688.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5665..5826
                     /locus_tag="ACL8A1_002057"
     CDS             5665..5826
                     /locus_tag="ACL8A1_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202758.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
BASE COUNT         2191 a          817 c          997 g         1822 t
ORIGIN      
        1 tccttttttg aaaaaattat attattaaat ttgtaactgt atctattgac aatgataatt
       61 attatcgata caatagactt gaaatatgtt taaggagttt ttatgaaaaa caaatttttt
      121 ctaatagcta ttttagctat gtgtatagtt tttagcgctt gttcttctaa ttctgttaaa
      181 aatgaagaaa atacttctaa agagcatgcg cctgataaaa tagttttaga tcatgctttc
      241 ggtcaaacta tattagataa aaaacctgaa agagttgcaa ctattgcttg gggaaatcat
      301 gatgtagcat tagctttagg aatagttcct gttggatttt caaaagcaaa ttacggtgta
      361 agtgctgata aaggagtttt accatggaca gaagaaaaaa tcaaagaact aaatggtaaa
      421 gctaacctat ttgacgattt ggatggactt aactttgaag caatatcaaa ttctaaacca
      481 gatgttatct tagcaggtta ttctggtata actaaagaag attatgacac tctatcaaaa
      541 attgctcctg tagcagcata caaatctaaa ccttggcaaa ctttatggag agatatgatt
      601 aaaattgatt caaaagcctt aggtatggaa aaagaaggtg atgagttaat caaaaatact
      661 gaagctcgta tatccaaaga attagaaaaa catccagaaa tcaaaggaaa aatcaaagga
      721 aaaaaagtat tatttactat gattaatgct gcagatacat caaaattctg gatttatact
      781 agcaaagatc caagagcaaa ttatttaaca gatttaggtc tagttttccc tgaatcatta
      841 aaagaatttg agagtgaaga tagttttgca aaggaaattt ctgcagaaga agcaaataag
      901 ataaatgatg ctgatgtaat cataacttat ggtgatgata aaactcttga agctttacaa
      961 aaagatcctc ttttaggtaa aataaatgca attaaaaatg gtgccgttgc tgtaattcca
     1021 gataatacac cgttagcagc ctcatgcact ccaacaccac tttcaataaa ctatactatt
     1081 gaagaatacc taaatctttt aggaaatgca tgcaaaaatg cgaaataaaa aacaaataaa
     1141 cctaggcata atttttgtaa tctgcctagg tcttcttatt acaatatttt tgtcattaaa
     1201 gcttggaaca aaagaaatta atatcagaga ttttttagca gcttttggaa tgggcaatac
     1261 aaatgatgat tttattaaat caattatata taaaagaata cctagaacta tttttgcaat
     1321 tttagcaggt tctagtcttg ccataagcgg tgtattgatg caatcagtta ctagaaaccc
     1381 aatagctgat ccaggtatac tcggtataaa cacaggagca agtcttagtg tagtaattgg
     1441 tctttctttt ttaggaattt catcaagcat aagccatata agttttgcaa tcattggtgg
     1501 cttagtaagt gcaatttttg tatacgcgat tgctgtaagc ggaaaagcag gccttacccc
     1561 tataaaactt gccttatcag gaacttgtgt tagtatggct ttaagcagtt ttgtaagttt
     1621 tttaatttta ccgaataata acgtcttaga caaatttaga ttttggcaaa taggtagcct
     1681 tggagcagct acattatctt ctatatctac actactacct tttataattt taggtcactt
     1741 gatagctata tttatttcat cagatttaaa cgctttagct atgggtgatg aaatggctgt
     1801 tggtcttgga gttaatgtta ataggataag atcacttgca ataattgcaa gtgtgctttt
     1861 atgttcaagt attactgcaa ttggtggacc tattggcttc gtaggtctta tagttcctca
     1921 cttttgtggc ttatttataa gcaaagatat acgcacaatg accatttctt catcttttat
     1981 aggtgcagag ctcttgctta tatgtgatat aatcggccgt atgttaggta aaccaggtga
     2041 aattgaagta gggataatta ctgcaataat cgggggtcca gtacttattt atgtaactat
     2101 gaaaaataga ggggttaata actaatgcaa aatttaatta taggtattca aaaaagaaaa
     2161 aatagaataa cactattttc ctcactattt ttattaataa taatcagtct atcatttttc
     2221 attttactta tcggagatga aagttattct ttttcaactt tgattaaagt cttaaatagt
     2281 gaaactgttc ctggagctag tttttcgatt atggaaatta gattaccaaa attattagca
     2341 ggaattatag ctggctggtc ttttggattg gcaggattta tctttcaaac tatgttaaga
     2401 aatcctcttg caagtcctga tataatcggt gtcacaagtt cttcatctat tgcagcggtc
     2461 ttttgcatat tggtattaaa aacaaatagt ttaactactg gaattatttc aataacttgt
     2521 ggactaacat catctttaat attattttta ctagctaaaa aagatggttt ttcagcagca
     2581 agactgataa tattaggtat tggttttcaa gctgtcacaa gagcaggcac ctcattttta
     2641 ttgttgaaag tagcaagata tgaattacaa gaagttatga gatggctcag tggctcttta
     2701 tcttttacaa agttagatga catacctctt gttctaatag taagtattat tgctactata
     2761 ttagttttat tttttaataa aagactagaa attattgaac ttggtgaaga aatagcaatc
     2821 ggacttggag caaatcccga gctttcaagg cttgttttaa ttttttgcgc tgtatcttta
     2881 actgcttttt ctacttcaat tacaggacca atagcttgta tatctttttt agctggtccc
     2941 atagccttaa atattggcaa gaaaagaagc ccaatattag ctggattggt tggaatttta
     3001 ctagttttgt tatcagacat attctctcaa aatattttac cagctagata tccagtaggt
     3061 gttgtaactg gcttgttagg ttcaccatac ttaatatact tactaataaa aatgaacagg
     3121 aggaatatat aatgaaaggt ctgtggtcaa ataatcttac ctgcggttat gatgaaaaaa
     3181 taatcttgga aaatataaat ataaaaatac ctgaagaaaa aatatcagtt attattgggt
     3241 caaatggttg tgggaaatca acactcatta aaaccttgtc tcgacttata aagccattag
     3301 agggagaagt attgctcgat aataaatcaa ttaattctta taaagaaaaa gatttagcaa
     3361 aacacatagc tatattacct caatctccaa taatccctga atcaataaca gtagctgatc
     3421 ttgtaagccg tggtcgtttc ccctacagaa agccttttaa gagtcttgga aaagatgacc
     3481 ttgaaataat aaacagatca atggttaagg ccaatgttga agatctagca aataacctag
     3541 ttgaagaact ttctgggggt caaaggcaaa gagtatggat agctctagcc ctagcccaag
     3601 atacaagtat cctactttta gatgagccaa ctacttactt ggatatctca tatcaaatag
     3661 aactattaga cctcttgact gatctaaacc aaaaatataa gacaaccatt tgcatgattt
     3721 tgcacgatat aaatctaaca gcaagatacg ctgattacct atttgcaatt aaagaaggta
     3781 aacttgttgc agagggaaag cctgaagata tactaaatga taaactagtt aaagatatct
     3841 ttaatcttga agcaaaaatt atacgtgacc ctatttccaa ttcgcctcta atgattccta
     3901 ttggcaagca ccatgttagc tcttaataat tgaatatcgg aattcattta aaagtcgcaa
     3961 attttgaact gaccctcaaa agttaggcca aaaaactaac taaaggaggt cagtttttta
     4021 atggcaaata cagcacagaa tttaagataa aattagtaaa agaatatcat gaatgaaaaa
     4081 taggatatag agaataacag caatattaaa aagatcagga ctgataataa acaaaaagaa
     4141 agtacaacga ctagttcaaa agctaaaact tcaagtaaaa agttattcaa gaaaatctag
     4201 aaaatactca tcctacaaag gacaggtagg aaaaatatcg gacaacaaaa taaaaagaaa
     4261 tatttattac cgaaaaaaag gggaaatcga gtcaagtacc aacaggctca tctattccaa
     4321 aaagatattt actcgatgga aaaactccta gaattagtgc tacaaatatt gacaatggta
     4381 tcttaggtta ctacgaagat atagatgata aaaattatag agtttttgaa aattttatat
     4441 ctgtttcatt tttaggagca gtcttttatc ataaatataa agcaagccta gatatgaaaa
     4501 ttcattgttt aaaattaaaa aataaagaat tgaataaaga agtagcattc tatttaactt
     4561 caattattag gcaagcttta aaaaatactg aatataaaga tcaaatatct agcacagtat
     4621 tgccagatat caaaattaaa ttaccgatag acagtagagg tactccagat tggaattaca
     4681 tggaaagata tagagataga taaagagaga gggagagata gataaaaagt ccacccccat
     4741 tttttatgga gaattcaaaa atgtaaggtt aagcaaagaa gaatataaaa atctaaaaga
     4801 aaaattaaac tcacacacag atataatgat aaataaacta tccagataca tggaaagcag
     4861 tggtaagacc tatcaaaacc actatgtgac aatcttaaaa tggtatgaag aagataaaga
     4921 caaactaaga cagaaaggtt taaataaaaa aatgaattat gatgtaggag aatctttata
     4981 gataaaaaga ggtggaaagg cactattaaa gactttaagt ctaaaaaggt atcaaagtat
     5041 gccagagata aaaaaatata aatatgacac tttaaaggcg attagaaaat taaagaaatg
     5101 ctagaagact tttagatgac agttaccaag agggtctaat tttcactgct acttatgcag
     5161 tgggaatttg acctttttat ataagcaaat tctatttcaa aattggttac caagaaagac
     5221 ttcttttcac tgttaagtat aagagggtaa tttatagaat tttgttacat taagataaat
     5281 attttctgat ggtcaaattt taaaaagatt aataactctc gataaatttt gaaccttaac
     5341 aattgaatag accaagacaa gacgttaatt atttttggag cgtaataacc tatccgtcaa
     5401 gatctttcgg ctgaataatt cggcaaaaaa agttctgcta agtaaaaaga aagtgagcga
     5461 taagtaagag tttgaatata gggagggata ccctatccgc tatgaagaaa atgaggataa
     5521 tgatacttct aaggttgaag ccggctcatc cggaatagag gcggaggtga gattcctatg
     5581 ttgctaatcc aaagcacttg tcaatgtcaa aaaacttaat taaacaaata attattagat
     5641 ataaaacttt agaggaggta tatattggac aatgactgga atggattggc tgatttaata
     5701 gcaaatctga ttgcaaaata tgctggtgct ttagatttag ataatctacc cgatccaacg
     5761 ccagcaaaaa atcaagagat gaaaaatagt tttgacatgg caaaaacaca aattgagacg
     5821 gactaat
//
LOCUS       DBERMR010000083         5790 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.083, whole genome shotgun sequence.
ACCESSION   DBERMR010000083 DBERMR010000000
VERSION     DBERMR010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.083"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..189)
                     /locus_tag="ACL8A1_002058"
     CDS             complement(<1..189)
                     /locus_tag="ACL8A1_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202759.1"
                     /translation="MNSFRSEYLKFIYNKWLLLTVLATIILVPLFVIFLHETPSYITE
                     DYVLTQILESFYLGQAGII"
     gene            complement(186..977)
                     /locus_tag="ACL8A1_002059"
     CDS             complement(186..977)
                     /locus_tag="ACL8A1_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202760.1"
                     /translation="MISSIKSHNILSELYKIFYSRSTQIVLPIVFILQPLLSYISSKQ
                     ILTVGLNATPETNSSLAESIPPIEYIGFDAILLGLFAMIILGAIIGSMEYKNSSLRTS
                     LLLCSNKAVFFVIKFFVTSVFIFVVSCVSIYLSIASAQFGLGREGLNPLILSNNVWYF
                     ILLGSLSWSLLTVLSYSIAFYFKSSLGSLLFLLPQVYNLGSFLSDRIQLAKYLPVSLG
                     QDLIATSPLKITTPGRSVLFLSIWVLVISSFAYYKFLKKDVGNVI"
     gene            complement(967..1683)
                     /locus_tag="ACL8A1_002060"
     CDS             complement(967..1683)
                     /locus_tag="ACL8A1_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1202761.1"
                     /translation="MTNKIEIRNVTKTYKDGDILKNVSFTAEEGEVTAFLGPNGAGKS
                     STLRILLGLDRSSLGTALIGGKKYCDIERPLLTVGASFDGVGAPSDRTVFQHLKIAAA
                     SNGIGFSRISEVLSITDIEHKRYSKISNLSLGEGQRLGIATALLGNPQYLILDEPTNG
                     LDPSGIRWFRNFIREQASTGKTVLLSSHILSEVEAVTDKVVFIDKGSIIASGTLHNIM
                     NGFDNLEDVFFHLTGDDYDK"
     gene            complement(1696..3918)
                     /locus_tag="ACL8A1_002061"
     CDS             complement(1696..3918)
                     /locus_tag="ACL8A1_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HGS1202762.1"
                     /translation="MDMQLNQESYSYVQLKYRLLAQIALLISYPLTKFSPKRIELLIN
                     KLSNSKLSATENEAILARDAICTVSHKCRNQDGCVKRSLGVIMFLLLLNKRASWCTGF
                     AMNPFRSHAWVEVNGNPIKELEEVSSYERVVKTLDDKNEERKHMISENIEKVDDTMTN
                     VKFRDLLVLIENKRKYFLIVLCLEILTSILTLLQPELLSKVISQKGVNLFNNPTIYML
                     ITITVGSTIISSLQYYFLQLMGESAVFQSRKNLIIQFLKLPIYKYNDLSAGDLISRFS
                     SDTSKLRAGIIQSTVALTSGLFLSLGAIFTLFLKDTYLAIITIISIALSFLFILLMSS
                     LIQTASYKAHQGLGKMTAYLNRLIVGIRTIRSTNETNSELSKMLSEVQEVKNLGIGVA
                     RVQSIMTPISNFSLQLCGIIILGIGGYRVSTGQMSIANLTSFMLLMYIAISPVGQIFS
                     SVTTISEALGALSRITEIIDLPKEEDNDIILNLESKSDRSKAIEFVNVTFSYDSYQFQ
                     DIQSDDNYILKDITFEINSGDYVSIVGPSGAGKSTLLYLIERFYDTTSGGIKIFGQDF
                     RTISRETLRSNISYVEQNSPLVAGTILENLKMGNSNVTYDECCDALEKVGLEHLIQRG
                     ASGIESPISEGGFNLSGGERQRLAMARAILSDAKILLLDELTSNLDSLSEKKMKEAIK
                     SMRGERTIIMVAHRLSTILDSDEIFVLEHGRIVGNGTHMELLESVPLYKELAKEQFLV
                     "
     gene            complement(3922..4173)
                     /locus_tag="ACL8A1_002062"
     CDS             complement(3922..4173)
                     /locus_tag="ACL8A1_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012775110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HGS1202763.1"
                     /translation="MFNKESKSRLKIIKQDTGAIVFDKNQGIYFQTNDVGVKILELLS
                     KNESEEEIVSAISVAYSIEIDVAKQDVKDFIQSLKKGGL"
     gene            complement(4188..>5790)
                     /locus_tag="ACL8A1_002063"
     CDS             complement(4188..>5790)
                     /locus_tag="ACL8A1_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HGS1202764.1"
                     /translation="GVCSIIEQNDISSFEEIILALQDYVTNGLIIVEMAERTYIISPY
                     LDSRLSWFENNKNIIFSDSSVEIAKYLNLKLSTKRLASQFIFGLPYYPFQTISLWEEL
                     VNIRPLNYLEISEKGCLEKRVPSYEVDTKDFNKLIIKIKEEFLNSINHDLNMRNEVSS
                     DVSGGVDSATIAFTLNKLIPDFSILHAESSTTANSDTKWATFIAKSLGRELKKFDSIE
                     ITEKRFAIDEKYINDIMPSSPLLWADSEGYLKSVIGYQKGRKQPTHFLGIGGDELFTP
                     MPSNSWNIVRQENLGGLLYALKYSLIMRRPFFSCLLDLLDNRGYSETVIKNLEIVFNE
                     SSAPIKRELGWVDGLQVPDWLSEKSKQESQIFLNSLLFSNSEPIISDRTVFQMIQSLI
                     FQKSVLRQIQLTTNSIYWATPFLHKKLIEICLQIPAKYKVSSKLTKPILQKALKGIVP
                     IEVFNRGFKGDYSDALYSGYREAVRKNFHNLEQFELVKMGIIDVEKLKLEMSLPAGNP
                     SKIDYFEKLCSVERWIRQIKLYMKN"
BASE COUNT         1925 a         1087 c          758 g         2020 t
ORIGIN      
        1 aataattcca gcttgtccaa gatagaagct ttctaagatt tgcgttaaaa cataatcctc
       61 ggttatataa cttggagttt catgtaaaaa aataacaaat aaaggcacta aaattatagt
      121 tgctaataca gtcaataata gccatttatt ataaataaac tttaaatact cacttctaaa
      181 agaattcata taacattacc tacatctttt ttcaaaaatt tgtaatatgc aaaactacta
      241 attactagca cccaaatact cagaaataaa acactgcgtc ctggtgttgt gatttttaaa
      301 ggagatgttg caattaaatc ttgacctaaa gatactggta aatactttgc aagctgaatt
      361 ctgtcagaaa gaaaactgcc taaattatag acttgtggta gtaagaataa taatgatcca
      421 agtgaggatt taaaataaaa agcaattgaa taggataata ccgttaataa cgaccacgac
      481 aatgacccca ataatataaa gtaccacaca ttattactca gtatcagagg gttcaatccc
      541 tctctcccta aaccaaactg tgcacttgca atagataaat agattgaaac acacgataca
      601 acaaaaataa atacacttgt aacaaaaaat tttattacaa agaatactgc cttatttgaa
      661 cataatagca aactggtacg taaagaacta tttttatatt ccatacttcc aataattgct
      721 cctaaaataa tcatagcaaa caagcccaat aatattgcat caaaaccaat atactcaatt
      781 ggaggtattg attctgctaa actactattt gtttcaggtg ttgcattcaa tccaactgtt
      841 aaaatttgtt tagaactgat ataagacaat aacggctgaa gaataaaaac aattggtaaa
      901 actatttggg ttgatctact ataaaaaatt ttatatagct ctgataaaat attatggctt
      961 tttatactac ttatcatagt catctcctgt taaatgaaag aaaacatctt ctaaattatc
     1021 aaatccgttc attatattat gaagtgtacc cgaagctatg atacttcctt tgtcaataaa
     1081 aacaacctta tcagttaccg cctctacttc tgaaagtata tgtgaagata acaatactgt
     1141 cttgccagta cttgcttgtt ctctaataaa atttctaaac catctaattc cactaggatc
     1201 taaaccattt gtaggttcat ctaaaattag gtactgggga tttcccaaca gcgctgttgc
     1261 aatacctaac ctctgaccct ctcctaatga aaggttgctt atttttgaat atcttttatg
     1321 ttcaatatca gtaatggata atacctctga tattctggaa aaacctattc cattactagc
     1381 agcagcaatt ttcaaatgtt gaaaaactgt ccgatcactg ggagccccta cgccatcaaa
     1441 agaagctcct acagttaaaa gaggacgttc aatgtcacaa tatttcttac ctcctattaa
     1501 agcagtacct aaagatgatc tatccagccc caaaagtatt cgtagtgtcg aactttttcc
     1561 tgcaccattc ggtcccaaaa aggccgtgac ttccccctct tcagctgtaa aggatacgtt
     1621 ctttaatata tccccatctt tataagtttt tgtaacattt cttatttcaa tcttatttgt
     1681 cataatacga tactcctata ctaaaaattg ttctttggcc agctctttat ataatggaac
     1741 agattccaat aactccatat gagtcccatt tcctacaatt cttccgtgtt ccaaaacaaa
     1801 gatttcatct gaatctaaaa ttgtagataa tctatgagca accattatta tggttctctc
     1861 acctctcata cttttgattg cttccttcat tttcttctca ctcagactat ccaaattcga
     1921 agttaactca tccagcaaga gtatctttgc atcggaaagt attgctcttg ccattgccaa
     1981 tctctgtctt tctcctcctg ataaattgaa ccctccttca ctaattgggc tttcaatccc
     2041 agatgcacct ctttgaatta aatgctccaa accaactttt tctaaagcgt cacaacattc
     2101 atcatatgtt acattactat tacccatttt tagattttct aagattgtac cagctactaa
     2161 tggtgaattt tgttcaacat atgaaatatt cgaacgtaaa gtctcacgac ttattgttcg
     2221 aaaatcttgt ccaaatatct ttataccacc actggtagta tcatagaatc tttcaatgag
     2281 gtaaagaagt gtacttttcc ccgcaccaga cggaccgaca atactgacgt aatctccact
     2341 atttatttca aaagtaatgt cctttaatat gtaattatca tccgattgaa tatcttgaaa
     2401 ttggtaagag tcatatgaaa aagttacatt cacaaattca attgctttgc ttctgtctga
     2461 cttagattct agatttaaaa tgatatcatt atcttcttct tttggaagat caataatttc
     2521 agtaattcta cttaaggctc ccaaagcttc tgaaatagtt gtgactgacg aaaatatttg
     2581 tcctactggt gaaatagcga tatacattaa tagcataaaa gaagttagat ttgcaataga
     2641 catttgccca gtagaaacac ggtagcctcc aattcccaaa ataattatcc cacacaattg
     2701 caagctaaaa tttgatattg gtgtcattat tgattgcact cttgcaactc caattcccaa
     2761 gttttttact tcttgtacct cagataacat tttagataat tctgaattcg tttcatttgt
     2821 cgaccgaatt gttctaatcc ctacgattag cctatttagg tatgctgtca ttttccccaa
     2881 accctgatga gctttataac ttgccgtttg aatcaaacta ctcatcaata aaataaatag
     2941 gaatgataaa gctatagaaa taattgtaat tattgcaaga taagtatcct ttaaaaatag
     3001 tgtaaatatc gcacctaaac tcaagaacaa tccactcgtc agagcaacgg ttgattgaat
     3061 gatacctgct cttagtttac ttgtatcact gctaaagcga ctaattaggt ctcctgcaga
     3121 tagatcatta tacttataaa taggaagttt caaaaattga ataattaaat tttttcttga
     3181 ttgaaatact gcactttctc ccattaattg taaaaagtaa tattgcaaac tggaaataat
     3241 tgttgaaccc acggtaatag ttataagcat atatatggtt ggattgttga ataaatttac
     3301 tcccttttga gagattactt tagataaaag ttctggttgt aataacgtta aaatggacgt
     3361 aagtatttcc aaacataaaa cgattaaaaa atattttctt ttattttcta tcaaaacaag
     3421 taaatcacgg aattttacgt tagtcatagt atcatctact ttttctatat tttcagatat
     3481 catatgttta cgctcttcat ttttatcatc tagagttttc acgactcttt catacgatga
     3541 tacttcttct aactctttaa tcggatttcc gtttacttcc acccatgcat gagatctaaa
     3601 ggggttcata gcaaatccag tacaccaact tgcacgttta tttaaaagaa gtaaaaacat
     3661 tattactcct aatgatcttt ttacgcatcc atcttgattc ctacacttat ggcttacagt
     3721 acatattgca tcacgagcta atattgcttc attttctgtg gcagatagtt tcgagttact
     3781 taacttatta atcaaaagtt caattctttt aggcgagaac tttgtaagcg gataagaaat
     3841 cagtaaagct atttgtgcta gtaatctgta ttttagctga acataggagt atgactcttg
     3901 attgagttgc atatccataa tctatagccc tccttttttt agagattgta taaaatcttt
     3961 aacatcttgt tttgcaacat cgatttcaat ggaatacgca acagatatag cgctaacaat
     4021 ttcttcttcg ctttcatttt tagatagtaa ctctaaaatt ttgacaccta catcatttgt
     4081 ttgaaagtat atcccttgat ttttgtcgaa tacaattgca ccagtatctt gctttattat
     4141 tttcagtcta cttttcgatt ctttattaaa cataattgta aacctcacta gttcttcata
     4201 tataatttga tctgccgtat ccaacgttct accgaacata acttttcaaa ataatcaatt
     4261 ttactcggat ttcccgcggg taatgacatc tctaatttta acttctcaac atcaataata
     4321 cccattttaa ccaattcaaa ctgttctaaa ttgtggaagt ttttacgaac tgcttctcta
     4381 tatccactat ataaggcatc tgaataatcc cctttgaaac ccctgttaaa aacctcaatt
     4441 ggtacgattc ccttcaatgc tttttgcaat atcggttttg ttagttttga actaacttta
     4501 tattttgctg gaatttgaag acaaatttct attaattttt tatgtaaaaa tggagttgcc
     4561 caataaatgg aattagtggt taattgaatt tgtcgcaata ctgatttttg aaatattaag
     4621 gattgaatca tttggaagac tgtcctatca gatattattg gctcagaatt ggaaaataat
     4681 aacgagttta aaaaaatttg cgattcttgt ttggattttt cactgagcca atctggtact
     4741 tgtaaaccat ccacccatcc cagttctctc ttgataggtg cagaagattc gttgaaaaca
     4801 atttctaaat ttttgatcac agtttccgaa tatcctctat tatctaacaa atccaatagg
     4861 caactaaaaa atggtcttct cattattaaa ctatatttca gagcataaag tagtccacct
     4921 aaattttcct gacgaacaat attccaggaa ttagacggca tcggtgtaaa taattcatca
     4981 ccaccaatac ctagaaagtg agtcggttgt tttctacctt tctggtaacc aatcactgat
     5041 ttcaaatatc cttctgaatc cgcccacaat aacggagaac ttggcataat atcattgata
     5101 tatttttcat ctatcgcaaa tcttttttct gttatttcta ttgagtcaaa ctttttcaac
     5161 tccctaccaa gacttttagc aataaatgtc gcccatttag tatcactatt agcagtggta
     5221 cttgattcag catgcaaaat agagaagtca ggtatcagtt tattcaatgt aaaagcaata
     5281 gttgcagaat caacacctcc tgatacatca ctcgacactt catttctcat attcaaatca
     5341 tgattaatgc tatttaaaaa ctcctctttt atttttatta ttagtttatt aaaatctttt
     5401 gtgtctactt cataactcgg aactcttttt tctaaacaac ctttttcaga tatttctaaa
     5461 tagtttaaag gtcttatatt cactagctct tcccagaggc taattgtttg aaatggataa
     5521 tatggaagtc caaaaataaa ttgacttgct aaacgcttcg tagagagttt taaattcaaa
     5581 tattttgcaa tttctacaga tgaatcacta aaaattatat tcttattatt ctcaaaccaa
     5641 gacagtcttg aatctaaata aggtgaaata atatatgttc tttcagccat ttcaacaata
     5701 attaaaccat ttgtaacata atcttgaagt gctaatatta tttcttcaaa actactaata
     5761 tcattttgtt ctataattga gcatactcct
//
LOCUS       DBERMR010000084         5712 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.084, whole genome shotgun sequence.
ACCESSION   DBERMR010000084 DBERMR010000000
VERSION     DBERMR010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5712
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.084"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            1..203
                     /locus_tag="ACL8A1_002064"
                     /pseudo
     CDS             1..203
                     /locus_tag="ACL8A1_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            358..558
                     /locus_tag="ACL8A1_002065"
     CDS             358..558
                     /locus_tag="ACL8A1_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202765.1"
                     /translation="MVVSASASSTSPQAQEQVDKSELRALSQELDQHLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            583..999
                     /locus_tag="ACL8A1_002066"
     CDS             583..999
                     /locus_tag="ACL8A1_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transmembrane domain-containing protein"
                     /protein_id="HGS1202766.1"
                     /translation="MKAALDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETN
                     SVPAGVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKA
                     QNLLQETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            1230..1937
                     /gene="ccdA2"
                     /locus_tag="ACL8A1_002067"
     CDS             1230..1937
                     /gene="ccdA2"
                     /locus_tag="ACL8A1_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HGS1202767.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYGMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            1948..2505
                     /gene="sdbB"
                     /locus_tag="ACL8A1_002068"
     CDS             1948..2505
                     /gene="sdbB"
                     /locus_tag="ACL8A1_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HGS1202768.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            2880..4271
                     /locus_tag="ACL8A1_002069"
     CDS             2880..4271
                     /locus_tag="ACL8A1_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGS1202769.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIALPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKVPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4414..5331
                     /gene="adcAII"
                     /locus_tag="ACL8A1_002070"
     CDS             4414..5331
                     /gene="adcAII"
                     /locus_tag="ACL8A1_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HGS1202770.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDSDVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLDRVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTENNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            5339..>5712
                     /locus_tag="ACL8A1_002071"
     CDS             5339..>5712
                     /locus_tag="ACL8A1_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HGS1202771.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVAYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGG"
BASE COUNT         1744 a         1004 c         1215 g         1749 t
ORIGIN      
        1 taaaaggtca gttaagaaga ggcaaagtat ctggaagaag atatcagagg atttctttgg
       61 ttgcaggtct aacaaatggt gagttaatcg ctccaatgac ttacgaagag atgatgacga
      121 gcgacttttt tgaagcttgg tttcagaagt ttctcttacc aacattaacc acaccatcgg
      181 ttattatagt aaaacgaaat aagaatagga caaatcgatc aggacagtaa aatcgaattc
      241 taacaatgtt ttagaagtcc atatgtacta ttctagtttc aatataccat aactgctcct
      301 agtcaacctg caagtcctgt cccagctcca agctcagctc cgactcaaga tcctagagtg
      361 gtagtaagcg cttcggctag ctcaacgagc ccacaagctc aagagcaagt tgacaagtct
      421 gaacttcgtg ccttgagtca agagttagac caacacttga aagctttggc aacagtatct
      481 gatccgaaaa tcgatgcaac caaggctgtc ctcctagatg ctcaaaaagc tccggaagat
      541 agtgccttga cagagtaagg tctgcatact gcagtagagt ctgtgaaggc tgccttggac
      601 tctctcaaag atgtgaaagc aaatgctagc gacagcaagc ctgcacagga caagaaggat
      661 gcaaaacaag gaacggaaga tagtaaggat tcagataaga tgactgaaac aaactcagtt
      721 ccggcaggag tgattgtggt cagtctactt gccctcctag gcgtgattgc cttctggctg
      781 attcgccgta agaaagagtc agaaatccag caattaagca cggaattaat caaggttcta
      841 ggacagctag atgcagaaaa agcggataaa aaagtccttg ccaaagccca aaaccttctc
      901 caagaaaccc ttgatttcgt gaaagaagaa aatggctcag cagagacaga aactaaacta
      961 gtagaggagc ttaaagcaat ccttgacaaa ctcaagtaaa acatgattta tgaaatgtca
     1021 gacttaaaag acattttcgt tcacaaaagc agcgacgagt aatccttgtc gttgcttttt
     1081 ggctattttg ggggataaga tccgccttga ctagcaaaag aaaagattgg tccaatagtg
     1141 cccgatacga ttttgcgtag cagaaagctt acaaataaaa ttgaatatag taaaatagga
     1201 ttagaattat taagaaagtt ggttctttat tggaaacgat agtattttta atctctgttt
     1261 ttctagcagg tgttttatcc tttttttctc cttgtatttt tcctcttctg ccagtctatg
     1321 ctgggatttt attggatgat caagaaagtg caaaaagctt ttctttgttt gggagaaagg
     1381 ttctctggtc aggcttgatt cgaacacttt gctttatcgc tggtatctct ctcattttct
     1441 ttattctagg ctttggtgct ggttactttg gtcatattct ctatgcaaat tggtttcgat
     1501 atggcatggg agctattatt atcattttgg gtcttcatca gatggaaatt tttcatttga
     1561 agaaattaga agttcaaaaa agttttacct ttaaaaaatc agattctaat cgttattggt
     1621 cagctttttt acttggtatt acctttagct ttggttggac accttgtatt ggtccagttt
     1681 taagttctgt tttagcactt gcggcttctg gaggcaatgg cgcttggcaa ggagcgattt
     1741 atactctcat ttacactctg ggcatggccc ttcctttctt ggtattggca ctagcttcag
     1801 gtctagtcat gccatatttt agtaaaatca agcgtcatat gatgctacta aagaaaattg
     1861 gtggtttcct cattgtttta atgggaattt tgttactatt aggacaagta aatgttctag
     1921 ctggaatttt tgaataaagg aggacaaatg aaaaaagtaa tgtttgctgg cttaagtctc
     1981 ttgtcattag ttgtattgat ggcctgtggt gaggaagaaa ctaaaaagac tcaagcagca
     2041 caacagccaa aacaacaaac gactgtacaa caaattgctg ttggaaaaga tgctccagac
     2101 ttcacattgc aatccatgga tggcaaagaa gttaagttat ctgattttaa gggtaaaaag
     2161 gtttacttga agttttgggc ttcatggtgt ggtccatgca agaaaagtat gccagagttg
     2221 atggaactag cggcgaaacc agatcgtgat ttcgaaattc ttactgtcat tgcaccagga
     2281 attcaaggtg aaaaaactgt tgagcaattc ccacaatggt tccaggaaca aggatataag
     2341 gatatcccag ttctttatga taccaaagca accaccttcc aagcttatca aattcgaagc
     2401 attcctacag aatatttaat tgatagccaa ggaaagattg gaaagattca atttggtgct
     2461 atcagtaatg cggatgcaga agcagcattt aaagaaatga actagaatcg ataaaatctg
     2521 attacaagat gtagtcagat tttggtaact tctaaaacta acttctagtt tcccacaacc
     2581 tagcttttag tatgagaaaa acgtgtgaca ccagtggaaa tccgtcttag actaacttcc
     2641 actggttttc tttttcttga tatataaggg ttcaaaagcg aaaagactca gaaaaaagtg
     2701 cacgacaaat agccctaaaa cagagtcgtt ttagagtaac ttccagtttt gggagagaga
     2761 tggaagttac tttgagaagt tacggtagtc agattttttt agaaaaacat tttataaata
     2821 ttcacatatc tcctatattt atggtaaaat agaatcatca gtttattttg gagtcaaaga
     2881 tgaatatatt tagaacaaag aatgttagtt tagataaaac agagatgcat aggcatttga
     2941 agttatggga tttgattttg ctgggtattg gagccatggt agggacaggt gtctttacaa
     3001 tcacaggtac tgcagctgct acacttgctg gtccagcact agtgatttca atcgttattt
     3061 ctgccttgtg tgtgggatta tcagccctct tttttgcaga atttgcctcg cgagtacccg
     3121 ctacaggagg tgcctatagt tacctctatg ctatcttagg agaattccct gcctggttgg
     3181 ctggttggtt aaccatgatg gagttcatga cagccatatc aggcgtagct tcgggttggg
     3241 cagcttattt taaaggcttg ctctctcaat acgggatagc ccttcctcag gctttaaatg
     3301 gtacctttaa tcctcaagca gggacatttg ttgatttatt gcctattctt gtcttggtct
     3361 tggtgacttc gcttgtttta ctaaatgcca aggcagcctt acgctttaat tcgattctag
     3421 ttattttgaa attttccgct ttagctctct ttgttttagt aggaatttgg aatattaagt
     3481 ttgataattg gagcaatttt gctccttacg gttttggaca aatctatgga gctagtactg
     3541 gtattatggc tggtgcgtcc ttgatgttct tcggtttttt gggatttgaa tccatctcta
     3601 tggctgtaga tgaagtcaag actcctcaaa aaaatattcc tagagggatt gtcttatcgc
     3661 tttctatcgt aaccattctc tatgccttgg tgacccttgt tttgactggt gtggttcact
     3721 atagtcattt aaatgtcgat gatgccgttg cctttgccct tcgtagtgtt gggattagtt
     3781 gggcagccaa ctatgtgtca ttagtggcta tcttgacctt gattacagtt tgtatttcga
     3841 tgacctatgc cctatcgcgt atgatttaca gtttagcacg tgacggctta gtgcctgctg
     3901 cctttaagga actgacgaag actagcaagg taccaaagaa tgctactatc ttgacaggtc
     3961 tagcttcagc agtagcagca ggaatgttcc cactagccag tattgcagcc tttttaaata
     4021 tttgtacctt agcctacttg atcatgctgg cttatggtct gattcgctta cggaaagaaa
     4081 aaggaatgcc caaagctggg gaatttaaaa caccattggt acctctgtta ccaattttat
     4141 cgatcatcat ttgtttatcc ttcatgttac agtataatat gaacacctgg attgctttcc
     4201 tagttgcgtt gttagtagga agtattattt acttcactta tggctataag cattctacca
     4261 tagaagaata aagtgaaagt ctattgagtt gttgacactc agtagatttt tatatgtgaa
     4321 agaaatttca aattttttct aaaaatatct tgacagatta aattttcagg agtagaatat
     4381 ttactagtta attaaatggt taaggagttg ttcatgaaga aacaaaattt atttttagtc
     4441 ctgttaagtg tctttctttt gtgcttgggg gcttgtggtc aaaaggaaag tcagacagga
     4501 aaggggatga aaattgtgac cagtttttat cctatctacg ctatggttaa ggaagtatct
     4561 ggtgacttga atgatgttcg gatgattcag tcaagtagtg gtattcactc ctttgaacct
     4621 tcggcaaatg atatcgcagc catctatgat tcagatgtct ttgtttacca ttctcataca
     4681 ctcgaatctt gggcaggaag tctggatcca aatctaaaaa aatctaaagt gaaggtttta
     4741 gaggcttcag agggaatgac tttagaccgc gtccctggac tagaggatgt ggaagcaggg
     4801 gatggagttg atgaaaaaac gctctatgac cctcacacat ggctagatcc tgaaaaagct
     4861 ggagaagaag cccaaattat cgctgataaa ctttcagagg tggatagtga gcataaagag
     4921 acttatcaaa aaaatgcgca agcctttatc aaaaaagctc aggaattgac taagaaattc
     4981 caaccaaaat ttgaaaaagc gactcagaaa acatttgtaa cacaacatac agccttttct
     5041 tatctagcga agagatttgg gcttaatcaa cttggtattg caggtatctc tcctgaacaa
     5101 gaaccaagtc cacgacaact aacagaaatt caggaatttg ttaagaccta taaggttaaa
     5161 acgattttta cagaaaataa cgcttcttca aaagtagctg aaactcttgt caaatcaaca
     5221 ggtgtgggtc ttaaaactct gaatccttta gagtcagacc cacaaaatga caagacctat
     5281 ttagaaaatc ttgaagaaaa tatgagtatt ctagcagaag aattaaagtg aggaaagaat
     5341 gaaaatcaat aaaaaatatc tagcaggttc agtggcagtc cttgccctaa gtgtttgttc
     5401 ctatgaactt ggtcgtcacc aagctggtca ggttaagaaa gagtctaatc gagttgctta
     5461 tatagatggt gatcaggctg gtcaaaaggc agaaaatttg acaccagatg aagtcagtaa
     5521 gagagagggg atcaacgccg aacaaattgt tatcaagatt acggatcaag gttatgtgac
     5581 ctctcatgga gaccattatc attactataa tggcaaggtt ccttatgatg ccatcatcag
     5641 tgaagaactt ctcatgaaag atccgaatta tcagttgaag gattcagaca ttgtcaatga
     5701 aatcaagggt gg
//
LOCUS       DBERMR010000085         5222 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.085, whole genome shotgun sequence.
ACCESSION   DBERMR010000085 DBERMR010000000
VERSION     DBERMR010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5222
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.085"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(367..1095)
                     /locus_tag="ACL8A1_002072"
     CDS             complement(367..1095)
                     /locus_tag="ACL8A1_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGS1202772.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRYSS"
     gene            1254..2663
                     /locus_tag="ACL8A1_002073"
     CDS             1254..2663
                     /locus_tag="ACL8A1_002073"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HGS1202773.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLELDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWLERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            2681..3976
                     /locus_tag="ACL8A1_002074"
     CDS             2681..3976
                     /locus_tag="ACL8A1_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGS1202774.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            3981..4289
                     /locus_tag="ACL8A1_002075"
     CDS             3981..4289
                     /locus_tag="ACL8A1_002075"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS1202775.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            4286..4594
                     /locus_tag="ACL8A1_002076"
     CDS             4286..4594
                     /locus_tag="ACL8A1_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGS1202776.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1761 a          877 c          949 g         1635 t
ORIGIN      
        1 aataaattgg gagattgaaa ttcatttcta gcaatttgtt agaagtcaca gtctactatt
       61 ctaacctcag tatactatat gtgttacaag aaaagactca aaaacttctc gtcagtgtaa
      121 gaatatttga aacactattt aaaactaaat aactataaga agtagatcag gtgaacaatt
      181 tcagtaccta ctctataagc tatacgaaga tatttttcaa catgatggat aaaacgatca
      241 ctcttttaaa gccatcttat ttagaaaaga gaaaccattt aagcatagat gggaccttct
      301 tgccctgttt taaatttttc ttaaactaga gtctgtaaaa gattggcttc attttcacat
      361 tttctcctag gaggaatatc gtaatgcaaa agaattaaat tcaaataaat catagcgatg
      421 acgactaatc gagtactcaa acggagttcc gttatccaaa taagccactt gttctacttc
      481 aaataccggt tccgttggaa ggagctgcaa gtaatgttgc tcattttctg aactagaaga
      541 agctcgtaag atttttgtag cactggcaat atgcaatcct aactttcctt caatgtaatt
      601 gtaaatcgat ttttgtaaaa tatcttcagt aatacctgga ataacatcgg tactcatata
      661 agtttgttcc agaacataag gtttaccgtc aataatacgc aggcggtaaa tattataaac
      721 tggactctgc aaagcgacct gtagtttttc tgctaaaaat tcatttgcaa attctaattt
      781 gaagtgaata atcctcgagt caattgtaga atgagcatct tcagatattt ttgtcagtcc
      841 ccggatatct ctttctggaa cgattaattt tctttttgag ctgccacgag agagaacaaa
      901 ggttccctgc ccacgttttc taaaaattaa gccctcagaa actaacaagt ctaaagcttt
      961 tttgatcgtc attcgactac aggcaaactg tttagctaat gtcatttcat caggtaattg
     1021 ttcgttcatg gtatatttat tacaaagaat cttttcacga atctcattgt aaataaataa
     1081 atattttgta ctcatttaat ctggttctct ctataatatt ctaatagtag tataacaaag
     1141 ttcaagaaat gaggcaaaca caagatatgt atcaacaaat ttttaaaaat gtctagacaa
     1201 attttgaaat tatgctatac tttatgtata gaataataga aaggaggttg agcatgaaaa
     1261 aaaagttagt atttcctaat ctgttttggt ggggagcagc ttctagcgga cctcagacag
     1321 aaggtcaata tggaaaagta catgaaaatg tgatggacta ctggttcaaa acgcatccag
     1381 aagatttttt cgataatgtc ggacctcttg tagccagtaa cttttttcat acttacaccg
     1441 aagatttcca cttgatgaag gaaattggag ttaattcttt ccgcacttcc atccaatgga
     1501 gtcgactcat caagaattta gagacaggtg agcctgatcc aaaaggtatt gctttctaca
     1561 atgccatcat tgaagaagct aaaaagaacc agatggatct tgtgatgaat ttacatcatt
     1621 ttgatttacc agtggaactt cttcaaaaat acggtggttg ggaaagcaaa catgtagtgg
     1681 agttattcgt gaagtttgcc aagactgctt tcacatgctt tggagataag gttcattact
     1741 ggacaacttt caatgagcca atggtcattc cagaagcagg atacttatat gctttccatt
     1801 atccaaatct aaaaggaaag ggaaaagagg ccgtacaagt catctataat ctaaaccttg
     1861 ctagtgcaaa agtgattcaa ctatatcgct cattagaact tgatggaaag attgggatta
     1921 ttttaaactt gacacctgct tatccaagaa gtaattctcc agaagactta gaagcaagtc
     1981 gatttacaga tgacttcttt aacaaagtct tcttgaatcc agctgttaaa ggaactttcc
     2041 cagaaagatt ggtaaaacag ctagagagag atggcgtgtt atggagtcat accgaaaaag
     2101 agcttcaact gatgaaatca aatacggttg attttcttgg agtaaactac taccatccaa
     2161 aacgtgttca agcacaagca aatcctgagg aatatcagac gccctggatg ccagaccaat
     2221 acttcaaaga gtatgaatgg ctggagcgtc gcatgaatcc atatcgtggt tgggaaattt
     2281 ttccgaaagc catttatgat attgctatga ttgtgaagga agaatatggt aatatcccat
     2341 ggtttatcag tgaaaacgga atgggtgttg aaaacgaagc acggtttatc gatgaaaatg
     2401 gagttatcga tgacgtgtat cgtattgaat tttatgaaga acatttaaga tggctacata
     2461 aagccattga agagggaagt cactgttttg gataccacgc ttggaccgca tttgattgct
     2521 ggtcttggaa taatgcatat aagaatcgtt acggatttat ctccgttgat ttagaaacgc
     2581 aaaagagaac catcaagagc tcaggaagat ggtatcgcaa agtaagtgac aataacggtt
     2641 ttgaagtaga aattgaggag taaagagagg agatattcat atgaaaaact caaaatttat
     2701 agaccaattt gccacctttg ctggtaaact agggaaccaa attcatttaa aaaccctaag
     2761 agatgcattc gtaacagtaa tgccattata tattttggca ggtttaatcg ttcttttgaa
     2821 caacacggta tttaagtgga ttttccaagg ggatacatta acaagattcc aatattgggg
     2881 aataacaatt gcaaacggta ctttaagtat ttcaggtatg attattgctg taatggttgg
     2941 ttatttctta gctaaaaaca gagatttcga aaacccgtta gcagcatcaa tgctatcatt
     3001 agtttcttta attgtgatga tgccaaatac agtttctgta gttcctgacg gagcaaaaga
     3061 tgctgtaaac atttcaggtg ttctttcatt caacaacaca ggtacaggcg caatgttcgc
     3121 cggggttatc gtagcgatta ttgcaacaga attattcatt gaattatcaa atgttaaagc
     3181 tttacaaatg aaccttggtg aaaatattcc accagctgtt agtagatcat ttagcgtatt
     3241 acttccagtc atgaccgtca tctccttatt tggggttgtt tcagcattat tgttcaatat
     3301 aactggaatg aacttaatct caatcattac aatctttatt caagaaccaa ttcgtcatat
     3361 tggtacaagc ttaatcgggg tcattattat ttactcttta ggaaatatgt tatggctatt
     3421 tggtattcac caagcagtta tttacagtgc catcctagaa ccattactat taattaacat
     3481 tactgaaaac atcactgcag caaataatgg acaagccatt ccacacatca tcaacctatc
     3541 acaaatacaa acattcgctt taatgggtgg tagtggatct acattatgtt tattaatagc
     3601 aacattctta gtgagtcgca atgctgtctc taaaaacgtg gctaaattat cttttggacc
     3661 tggtatcttc aatatcaatg aaccagtatt attcggttac ccaatcgttt ataacatttc
     3721 attagctatt ccatttatca cagttccagt ccttggtatt ttaatcagct acttagcaac
     3781 agttacagga ttcatgagtc ctgcatttat acaagttcct tggactacac cagtattctt
     3841 aaatgcatgg ttagcaacag caggggacgt gagagcagtt ctagttcaat tcatcatctt
     3901 tgcacttgga gttcttctat acattccatt tatcaaagtt aatgacaaag ttgttgaaca
     3961 agaaatggaa ggttaagatt atgaaacaaa ttttattagt atgtaatgcg ggaatgtcga
     4021 caagtatgct tgttaaaaaa atgcaacaaa gtgcgactga acgtgggatt gaaatctcta
     4081 tccaagcaaa atcaatgaca gaagctaaaa agaatatcca tgaagcagat gtcattctaa
     4141 ttgggccaca aattcgttat gagttacttg ctgtgaaaga aattgcagga aatatccctg
     4201 tagataccat tgatatgcgg gattacggaa tgatgaatgg agcaaaagtt ctggaacaag
     4261 cacttgcatg gataggagaa ataagatgac agaagaaatg gaatatatct gttttcagct
     4321 cattgccaat agtggagcag ccaaatcatc ctttattgaa gccatccaac tagctaaggc
     4381 aggaaattta aaagaagcga aaattaaagt tgaagaagcg gaagactcct tagtagaagc
     4441 gcacaagatt cactctaatc tcatacaaaa agaagctact ggagaaaaaa taggattctc
     4501 cttgctattt atgcatgcag aagaccaaat ggcttcaaca gagattattc agttgctatc
     4561 taaagaattc atcgatctct atcaaaataa ataggaacac tgtcattccc atctgtcttt
     4621 attaatgacg actacattgt aggaacctcc aaaagttgga caaaatgatt gaaaggattg
     4681 gatcctgcat tacacaagat ccaatccttt tcttttactc tagtttttat aatatgttga
     4741 atctagaata gtccactgta gtttctaaaa aattgctgga aatggatgtt aagctccaat
     4801 ttatttgttt atatcttatt tcagtttact atactagctt attattctag tgaaaaattg
     4861 aatgagccac tatttattta atatgtatga tgagggaatg actcaaaaac ttcttgtcga
     4921 tataagaata tctgagatag tattttaaaa cgaaaggact ataaaagctc atttccttcg
     4981 cacttatttt atagaaatct atcattatac tctttagaaa tcaaaaagca acacaccttg
     5041 tcatcgtttt atcgtaaatc tgttaatgag ggggctagtc ttatctgcaa tctcaaatca
     5101 gtgttttgag ctgaatggca ctcgttttct agtttgctct ttgattttaa ttgggcctta
     5161 gtaaatacta ctcttaaatt aaactatagt gaattgaaat aagatgtgaa caactctatc
     5221 ag
//
LOCUS       DBERMR010000086         5191 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.086, whole genome shotgun sequence.
ACCESSION   DBERMR010000086 DBERMR010000000
VERSION     DBERMR010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.086"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            1..71
                     /locus_tag="ACL8A1_002077"
     tRNA            1..71
                     /locus_tag="ACL8A1_002077"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33..35,aa:Glu,seq:ttc)
     gene            324..1869
                     /locus_tag="ACL8A1_002078"
     rRNA            324..1869
                     /locus_tag="ACL8A1_002078"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1921..1993
                     /locus_tag="ACL8A1_002079"
     tRNA            1921..1993
                     /locus_tag="ACL8A1_002079"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Ala,seq:tgc)
     gene            2118..5019
                     /locus_tag="ACL8A1_002080"
     rRNA            2118..5019
                     /locus_tag="ACL8A1_002080"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            5096..>5191
                     /gene="rrf"
                     /locus_tag="ACL8A1_002081"
     rRNA            5096..>5191
                     /gene="rrf"
                     /locus_tag="ACL8A1_002081"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1466 a         1054 c         1521 g         1150 t
ORIGIN      
        1 gtccgttggt caaggggtta agacaccgcc ttttcacggc ggtaacacgg gttcgaatcc
       61 cgtacggact atggtatgtt gcggttggaa cacttgatga aaaaaagttt aaaaaagctt
      121 aaaaatcttc aaaaaaagtg ttgacaagcg aaagcggctg tgatatacta atatagttgt
      181 cgcttgagag aagcaagtga caaagacctt tgaaaactga acaagacgaa ccaatgtgca
      241 gggcgctaca acataagttg tagtactgaa caatgaaaaa acaataaatc tgtcagtgac
      301 agaaatgagt aagaactcaa acttttaatg agagtttgat cctggctcag gacgaacgct
      361 ggcggcgtgc ctaatacatg caagtagaac gctgaaggag gagcttgctt ctctggatga
      421 gttgcgaacg ggtgagtaac gcgtaggtaa cctgcctggt agcgggggat aactattgga
      481 aacgatagct aataccgcat aagagtggat gttgcatgac atttgcttaa aaggtgcact
      541 tgcatcacta ccagatggac ctgcgttgta ttagctagtt ggtggggtaa cggctcacca
      601 aggcgacgat acatagccga cctgagaggg tgatcggcca cactgggact gagacacggc
      661 ccagactcct acgggaggca gcagtaggga atcttcggca atggacggaa gtctgaccga
      721 gcaacgccgc gtgagtgaag aaggttttcg gatcgtaaag ctctgttgta agagaagaac
      781 gagtgtgaga gtggaaagtt cacactgtga cggtatctta ccagaaaggg acggctaact
      841 acgtgccagc agccgcggta atacgtaggt cccgagcgtt gtccggattt attgggcgta
      901 aagcgagcgc aggcggttag ataagtctga agttaaaggc tgtggcttaa ccatagtagg
      961 ctttggaaac tgtttaactt gagtgcaaga ggggagagtg gaattccatg tgtagcggtg
     1021 aaatgcgtag atatatggag gaacaccggt ggcgaaagcg gctctctggc ttgtaactga
     1081 cgctgaggct cgaaagcgtg gggagcaaac aggattagat accctggtag tccacgctgt
     1141 aaacgatgag tgctaggtgt tagacccttt ccggggttta gtgccgtagc taacgcatta
     1201 agcactccgc ctggggagta cgaccgcaag gttgaaactc aaaggaattg acgggggccc
     1261 gcacaagcgg tggagcatgt ggtttaattc gaagcaacgc gaagaacctt accaggtctt
     1321 gacatccctc tgaccgctct agagatagag ttttccttcg ggacagaggt gacaggtggt
     1381 gcatggttgt cgtcagctcg tgtcgtgaga tgttgggtta agtcccgcaa cgagcgcaac
     1441 ccctattgtt agttgccatc atttagttgg gcactctagc gagactgccg gtaataaacc
     1501 ggaggaaggt ggggatgacg tcaaatcatc atgcccctta tgacctgggc tacacacgtg
     1561 ctacaatggc tggtacaacg agtcgcaagc cggtgacggc aagctaatct cttaaagcca
     1621 gtctcagttc ggattgtagg ctgcaactcg cctacatgaa gtcggaatcg ctagtaatcg
     1681 cggatcagca cgccgcggtg aatacgttcc cgggccttgt acacaccgcc cgtcacacca
     1741 cgagagtttg taacacccga agtcggtgag gtaaccgtaa ggagccagcc gcctaaggtg
     1801 ggatagatga ttggggtgaa gtcgtaacaa ggtagccgta tcggaaggtg cggctggatc
     1861 acctcctttc taaggataag gaactgcgca ttggtcttgt ttagtcttga gaggtcttgt
     1921 ggggccttag ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc
     1981 ccgctaggct ccattggtga gagatcacca agtaatgcac attgaaaatt gaatatctat
     2041 atcaaatagt aacaagaaaa taaaccgaaa acgctgtagt attaataaga gtttatgact
     2101 gaaaggtcag aaaataaggt taagttaata agggcgcacg gtggatgcct tggcactagg
     2161 agccgacgaa ggacgtgaca aacgacgata tgccttgggt agctgtaagt aagcgatgat
     2221 ccagggattt ccgaatgggg gaacccaaca ggtagtacct gttacccaca tctgttaagg
     2281 atgtgaggag gaagacgcag tgaactgaaa catctaagta gctgcaggaa gagaaagcaa
     2341 aagcgattgc cttagtagcg gcgagcgaaa cggcagaagg gcaaaccgaa gagtttactc
     2401 ttcggggttg taggactgca atgtggactc aaagattata gaagaatgat ttgggaagat
     2461 cagccaaaga gagtaatagc ctcgtattta aaatagtctt tgtacctagc agtatcctga
     2521 gtacggcggg acacgtgaaa tcccgtcgga atctgggagg accatctccc aaccctaaat
     2581 actccctagt gaccgatagt gaaccagtac cgtgagggaa aggtgaaaag caccccggga
     2641 ggggagtgaa atagaacctg aaaccgtgtg cctacaacaa gttcgagccc gttaatgggt
     2701 gagagcgtgc cttttgtaga atgaaccggc gagttacgtt atgatgcgag gttaagttga
     2761 agagacggag ccgtagggaa accgagtctg aatagggcgc cttagtatca tgacgtagac
     2821 ccgaaaccat gtgacctacc catgagcagg ttgaaggtgc ggtaagacgc actggaggac
     2881 cgaaccaggg cacgttgaaa agtgcttgga tgacttgtgg gtagcggaga aattccaaac
     2941 gaacttggag atagctggtt ctctccgaaa tagctttagg gctagcgtcg acattagaga
     3001 ttcttggagg tagagcactg tttgggtgag gggtccatcc cggattacca atctcagata
     3061 aactccgaat gccaatgaat tatggtcggc agtcagactg cgagtgctaa gatccgtagt
     3121 cgaaagggaa acagcccaga ccaccagcta aggtcccaaa ataattgtta agtggaaaag
     3181 gatgtggggt tgcacagaca actaggatgt tagcttagaa gcagctattc attcaaagag
     3241 tgcgtaatag ctcactagtc gagtgaccct gcgccgaaaa tgtaccgggg ctaaaacaat
     3301 ttaccgaagc tgtggatacc tttataggta tggtaggaga gcgttctatg tgtgatgaag
     3361 gtataccgtg aggagtgctg gaacgcatag aagtgagaat gccggtatga gtagcgaaag
     3421 acaggtgaga atcctgtcca ccgtaagact aaggtttcca ggggaaggct cgtccgccct
     3481 gggttagtcg ggacctaagg agagaccgaa aggtgtatcc gatggacaac aggttgatat
     3541 tcctgtacta gagtatgtag tgatggaggg acgcagtagg ctaactaaag cagacgattg
     3601 gaagagtctg tctaagcagt gaggtgtgaa ttgagtcaaa tgcttaattc tataacattg
     3661 agctgtgatg gggagcgaag tttagtagcg aagttagtga cgtcacactg ccaagaaaag
     3721 cttctagcgt ttaaacatac tctacccgta ccgcaaaccg acacaggtag tcgaggcgag
     3781 tagcctcagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
     3841 cgggagaagg ggtgctgact taaagtcagc cgcagtgaat aggcccaagc aactgtttat
     3901 caaaaacaca gctctctgct aaatcgtaag atgatgtata gggggtgacg cctgcccggt
     3961 gctggaaggt taagaggagt gcttagcgta agcgaaggta tgaattgaag ccccagtaaa
     4021 cggcggccgt aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac
     4081 ccgcacgaaa ggcgtaatga tttgggcact gtctcaacga gagactcggt gaaattttag
     4141 tacctgtgaa gatgcaggtt acccgcgaca ggacggaaag accccatgga gctttactgc
     4201 agtttgatat tgagtgtctg taccacatgt acaggatagg taggagtcta agagatcggg
     4261 acgccagttt cgaaggagac gctgttggga tactaccctt gtgttatggc cactctaacc
     4321 cagataggtg atccctatcg gagacagtgt ctgacgggca gtttgactgg ggcggtcgcc
     4381 tcctaaaagg taacggaggc gcccaaaggt tccctcagaa tggttggaaa tcattcgcag
     4441 agtgtaaagg tataagggag cttgactgcg agagctacaa ctcgagcagg gacgaaagtc
     4501 gggcttagtg atccggtggt tccgtatgga agggccatcg ctcaacggat aaaagctacc
     4561 ctggggataa caggcttatc tcccccaaga gttcacatcg acggggaggt ttggcacctc
     4621 gatgtcggct cgtcgcatcc tggggctgta gtcggtccca agggttgggc tgttcgccca
     4681 ttaaagcggc acgcgagctg ggttcagaac gtcgtgagac agttcggtcc ctatccgtcg
     4741 cgggcgtagg aaatttgaga ggatctgctc ctagtacgag aggaccagag tggacttacc
     4801 gctggtgtac cagttgtctt gccaaaggca tcgctgggta gctatgtagg gaagggataa
     4861 acgctgaaag catctaagtg tgaaacccac ctcaagatga gatttcccat gattatatat
     4921 cagtaagagc cctgagagat gatcaggtag ataggttaga agtggaagtg tggcgacaca
     4981 tgtagcggac taatactaat agctcgagga cttatccaaa gtaactgaga atatgaaagc
     5041 gaacggtttt cttaagttga atagatattc aattttgagt aggtattact cagagttaag
     5101 tgacgatagc ctaggagata cacctgtacc catgccgaac acagaagtta agccctagaa
     5161 cgccggaagt agttgggggt tgccccctgt g
//
LOCUS       DBERMR010000087         4886 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.087, whole genome shotgun sequence.
ACCESSION   DBERMR010000087 DBERMR010000000
VERSION     DBERMR010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.087"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..946
                     /locus_tag="ACL8A1_002082"
     CDS             <1..946
                     /locus_tag="ACL8A1_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HGS1202777.1"
                     /translation="YIIALPTDYDEKIGSFNTYEIEQTVSKILRVKPNGKIILKSTVP
                     IGFSNKLKRLFDTKNIIFVPEFLREGCSIYDNLYPSRIVVGDDTVEGRKIAELFLSIS
                     THSTANIRNVMLVSPTEAEAIKLFSNTFLALRVAFFNELDSFAERRSLNAEVVIKGVC
                     LDPRIGNFYNNPSFGFGGYCLPKDTKQLKKEFIEINAPVIEAIDISNTNRKQFIVKQI
                     LERKPKIVGIYKLGMKYNSDNYKESAILSIINELLIVGIKILVYEPNLNVSIDNVIFE
                     KNFELFTKQSDLIVANRWDRGLEAYKDKVYTRGIWIRD"
     gene            1341..2204
                     /locus_tag="ACL8A1_002083"
     CDS             1341..2204
                     /locus_tag="ACL8A1_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HGS1202778.1"
                     /translation="MIEKMELGEFYKELRLARKLKQTDVACEGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMRIGRKVVNRFAHQDIAALEQLLEEV
                     EQEQMAQTYRRLNAIVIKNALHSLDKSYPLAEEDSEFLTTYLYAIESWTWFELYIFCN
                     TMPFLSNQDLVFLSTALIEKSKEFKELVHNRLYMKQGLLNILSELMERKLFSYIPIFE
                     AELESMLRPYDVFEKVLWQFLKKMSIFLQTKGSNQKEIENFIQSLQVLENPQLTALFK
                     LRLQQYKALID"
     gene            2490..2690
                     /locus_tag="ACL8A1_002084"
     CDS             2490..2690
                     /locus_tag="ACL8A1_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone VP1"
                     /protein_id="HGS1202779.1"
                     /translation="MLNLQFAETMELTEAELETVYGGEFGNNAVIPAGAWGGFGTPWS
                     ITNFWKKNFNDRPDFDSDRRRY"
     gene            2757..3422
                     /locus_tag="ACL8A1_002085"
     CDS             2757..3422
                     /locus_tag="ACL8A1_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGS1202780.1"
                     /translation="MKKIILHRYFIIVFLLVIADQKFSVLVLRSDLVTGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVVWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEEIIHRGLVMTVLEKGKKWGLDVL
                     GSAILFGVSHISNHGWVLTDFFVYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            3494..4720
                     /locus_tag="ACL8A1_002086"
     CDS             3494..4720
                     /locus_tag="ACL8A1_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGS1202781.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFTKFLLYSIVSLSLSGQSFSLL
                     LVTFICLLNIVSDTLSAFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
BASE COUNT         1478 a          806 c          930 g         1672 t
ORIGIN      
        1 ttatattatt gcgcttccga cagattatga tgagaaaatt ggtagtttta atacatatga
       61 aatcgaacaa acggtatcga agattctgag ggtaaaacct aatggaaaga ttattttaaa
      121 gtcaacagtt ccgatcggct tttcaaacaa attaaaaagg ctgtttgata caaaaaatat
      181 catttttgtc cctgaatttt tgagagaagg ttgttctata tatgataatt tatatccaag
      241 tcgcatagtt gttggagatg acacagttga aggaagaaaa attgcagagt tgttcctttc
      301 gattagtact catagtactg ccaatattag aaatgttatg ttagtttctc ctactgaagc
      361 agaagcaatt aagctttttt ctaacacatt cttagctctc cgtgttgctt tttttaatga
      421 actagattct tttgctgaga ggagaagttt aaatgctgaa gttgtaataa aaggtgtttg
      481 tttagatcca agaattggaa atttttataa taatccttct tttggatttg gaggatattg
      541 tcttcccaaa gatactaaac aattaaaaaa agaatttata gaaataaatg ccccagtgat
      601 agaagcgatt gatatttcaa acacaaatag aaaacagttc atagttaaac aaatattgga
      661 acgaaagcca aagatagtag gaatatataa attggggatg aaatataatt cagataatta
      721 caaagagtca gctattttaa gtataattaa tgaactattg attgtcggta taaaaatttt
      781 agtatatgaa ccaaatttaa acgttagtat agataacgta atttttgaaa agaattttga
      841 actatttaca aaacaaagtg atctaattgt tgcaaataga tgggataggg gccttgaagc
      901 ttataaagat aaagtttaca caagggggat ctggataaga gattaactat tgatattaaa
      961 aaggcaataa tttataaaat ttgtacaaaa aaatgttaaa ttttcacatt ttcatagtat
     1021 actgtttaat agaaagtagg ttctttgtaa ccgctccatt ataaaatcct ttcactcttc
     1081 cggtatacta ttaggaaaaa cataactgga ggatattttg agctcacacc tcatcgttcc
     1141 agtggctgtt ccccaagctc gtgtactgcc caaaaaagtt agatattcta tttagaacga
     1201 ggattgagtt ctgtattgta caaggctcgg tccttttaga gtcagcttaa ggctggcttt
     1261 ttcaatcacc aaagtgtcag aatgttttga caaatgaacg caaataatga tataatatgc
     1321 aaaagctagg aggtggtagg atgattgaaa aaatggaact gggggaattt tacaaggaat
     1381 tacgtttggc gagaaagctt aagcagacag atgtggcttg tgagggtcta acagcttctc
     1441 agttgtccaa gtttgaacta gggcagtcta tgctgtctgc ggataagttg attctagcta
     1501 tccaaggtat aaatgtgacc tttgatgagt ttgggcacaa gctcaacaac tatcaagaat
     1561 ctccacatat gcgaatcggt cgaaaggttg tgaatcgctt tgcccatcag gatatagctg
     1621 ctttagagca gctattggag gaagtcgagc aagaacagat ggcgcagacc tatcgtcgtt
     1681 tgaatgctat tgtaattaaa aacgcactcc attccttaga taaaagctat ccgctagcag
     1741 aggaggatag cgagtttttg accacctatc tctatgctat cgagtcttgg acctggtttg
     1801 aactctatat tttttgcaat actatgccct tcctgagcaa tcaagatttg gtatttttat
     1861 caaccgcttt aattgagaaa tctaaagaat ttaaagagtt agtacacaat cgattgtata
     1921 tgaagcaagg actcttaaat atcttatcag aacttatgga gcgaaaactt ttctcttaca
     1981 tcccaatctt tgaagccgag ttggagagta tgctccgtcc gtatgatgtt tttgagaaag
     2041 tgttgtggca gtttttaaag aaaatgagta tttttcttca aaccaaggga agcaaccaaa
     2101 aagagattga aaattttatc caatctctgc aagtattaga aaatccacaa ttaacagccc
     2161 tttttaaact acgcttacag caatacaaag cacttatcga ttagttagaa atgggaaaaa
     2221 gataataatc tcagcaacga ttgtcaataa tgttaaaact tgtttcttca tatgacttcc
     2281 ctcctcatta gtaataactt tattatacca gggaaataat caaatatacc aaaatcgcaa
     2341 atctgaaatt tctataagaa aaaatatcaa atatgcgatt ttctaaaata agccaatttt
     2401 cgtgttatac tgtacttgta aagcacttga agcaaatcct aggtcgcaga agtggtttac
     2461 aaatgaagat aattgaagga gtgtaaaaga tgttaaattt acaatttgca gaaacaatgg
     2521 aattgacaga agctgagttg gagacagttt atggagggga atttgggaat aatgctgtta
     2581 tcccagccgg tgcttgggga gggttcggta caccttggtc aatcactaat ttctggaaga
     2641 aaaattttaa cgatagacct gactttgatt ctgaccgccg tcgttattaa tatccaaagc
     2701 ccttgccttt tcttgggtag gggctgtctt tatttcatag tataggagga aatagaatga
     2761 aaaagataat cttacaccgc tactttatta tagtttttct actagttatt gctgaccaga
     2821 agttcagtgt tctagtttta cgcagcgacc ttgttactgg gctatctgac tttgcctatt
     2881 atctgtcgga tatgatgttg aattttcttg tggttttatt tgctcttatt gctatgattt
     2941 ggtcggggaa atggcagaaa atcaatagta gaaagtttaa aggatcctat cttttctatt
     3001 ccttcctagc tctgcttgct tttgttgttt ggaatttcgt tacctttttt ctattcccac
     3061 ctactcgaaa tgaaatttct tatcaacatg ctgctccgac ttttacaggg gctacagcat
     3121 ttttgatgta ttttttctat cctgtgattg caggtcccat ttttgaagag ataatccatc
     3181 ggggattggt gatgacagtt ctggaaaaag gaaagaaatg ggggctagat gtacttggtt
     3241 ctgctatttt atttggggtc tcgcacatta gtaatcacgg ttgggtcttg acagactttt
     3301 tcgtctatat gggtggcgga cttatatttg cagtcttatt tagaatgaca aagtccattt
     3361 attggcctat tgggctgcat atagtctaca acggcattgg tcagcttttg atgttactgt
     3421 aattttggtt gttagtagta tcgcggtcta tttctgggga aagattccca tttgaactta
     3481 agaaggagtt gtgatgaaag tatttcttca aaatagagat tttaggcaat taactatcaa
     3541 ccagtggatt tcaacgcttg gggatacgat tttttatctg gcctttttga attatgtggc
     3601 agatgcgtct tttgcccctt tggcgatttt acttatcacg atttcagaaa cccttcccca
     3661 agttctacaa atctttctgg gagttttggc agattttcaa catcatcgtg tcttgaaata
     3721 cacagtcatt agttttacaa aatttttgct ttactctata gtgtccctat cgctttcagg
     3781 gcagtctttt tccttgttgc tagtaacctt tatttgtctg cttaacatcg tgtctgatac
     3841 attgagtgct ttttcaggcg ccatgctcac tccgattttc attagaatta ttgggcaaga
     3901 ccatctggca gaagctattg gatttaaaca gtcaactgtt agtttagtga aaacaatcag
     3961 taatatccta ggaggagtct tactaggcat tctatccatc cagtttattt ccttactgaa
     4021 cgctcttacc tttttaatcg catttttagg tatcctcttc ataaaaactg acctcttgaa
     4081 agtagaaaaa acgattagct atcaagaagg actctctgta aaatcctttt gccagcattt
     4141 gctccaatca tcgaaattga tatggaatat gaataaggtg ctcttggttt tgtttattat
     4201 ctctactagt caagcagtga taaatgttac agttcctgtc tctactctat ttttgaggaa
     4261 tcaacccttt ttgaatttac aaacaggtca atctcttgcc ttgctatcca cttttgaact
     4321 gtcagccctg attgtcggaa gccttgtaag tggctatctg caacatacca tttccataaa
     4381 aacagctctc tatgcctcac ttgtcatcca gttgcttctt ctagtaggat ttgccacagt
     4441 tcgttttgac tggattctca tctttagtac cttggatgcc tttttcgcag gtgttctctc
     4501 tcctagattg caagaactcg tttttaaaca aatacctgag gagtcaatgg gagcagttca
     4561 atcttctatc ggtgccatta cggttgtttt acctagctta tttacaatag ctttggtaac
     4621 cattgctact agctttggaa ctctggcagt tagctttgtt ttattactat ttcttctagt
     4681 tgcctttgtc atgcttctga atattcgtga aagtatttag tggagaattt ctataatata
     4741 tgtttgagac tggattgcca gtctttttat gatggtcaaa atcaacagac tttttctctt
     4801 ttctgaatgt gttatagtag actgaatcta aaatagtacg aaacaattgc taaaacattt
     4861 atagaaatta attttacttt cccaat
//
LOCUS       DBERMR010000088         4477 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.088, whole genome shotgun sequence.
ACCESSION   DBERMR010000088 DBERMR010000000
VERSION     DBERMR010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.088"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(99..332)
                     /locus_tag="ACL8A1_002087"
     CDS             complement(99..332)
                     /locus_tag="ACL8A1_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGS1202782.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            complement(338..1330)
                     /gene="plsX"
                     /locus_tag="ACL8A1_002088"
     CDS             complement(338..1330)
                     /gene="plsX"
                     /locus_tag="ACL8A1_002088"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HGS1202783.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVGIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTIDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLSGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            complement(1327..2097)
                     /gene="recO"
                     /locus_tag="ACL8A1_002089"
     CDS             complement(1327..2097)
                     /gene="recO"
                     /locus_tag="ACL8A1_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HGS1202784.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDERRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            complement(2094..3263)
                     /locus_tag="ACL8A1_002090"
     CDS             complement(2094..3263)
                     /locus_tag="ACL8A1_002090"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HGS1202785.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQAISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQVEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFASAAFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            3412..4422
                     /locus_tag="ACL8A1_002091"
     CDS             3412..4422
                     /locus_tag="ACL8A1_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HGS1202786.1"
                     /translation="MSFLSKNGAGILVCLLISILSWYLGGFFPVIGAPVFAIFMGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVILLGFGLNISQVFAVGQSSLPVILSTISIALI
                     VAYLFQRFFALDTKLATLIGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNDGFALFAGTAVNDTSSVTATASAWDSLYQTNTLESATIVKLT
                     RTLAIIPITLFLSYWQSREQKNKQGLQLKKVFPLFILHFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIVMAMSAIGLKTNLVAMVKSSGKSILLGAICWIAIILTTLGMQTLIG
                     IF"
BASE COUNT         1172 a         1165 c          846 g         1294 t
ORIGIN      
        1 agcccttgat aaccactgtc agccaagatt ttaccagctt gtccgatatt tctcatttct
       61 aaaaaccatc tacttccgtt gacttggaac tccgattgct attttccttg aatgatttta
      121 accacatctc ctacgttttg gagttggtca atttcttcat cgctgatttc gatactaaat
      181 tcatcctcca gcgtcaagat aaactccatc aaatcaactg agtcagcatc caagtcgtct
      241 ttcagactca aggattctgt cacgacaaag tcctctccct gtcgctcttg gataatggtc
      301 acaatacggt caaaaatttc tttttctgtc atctctttta ttctcctgaa aattcacgcg
      361 cagtctgggc aaccacgtct gtttctagca tggtacggat ctgacgaatc gtactataaa
      421 cagccttggc atcgcttgag ccatgagtct tgacaacagg tgccttaaca ccaaacaaga
      481 ccgctccacc aacatctgaa taattgagct gttttttcaa accactgagg ctgtccttga
      541 gaaggagggc acctagtttc gctcgaagac caccacctgt aatagctgtc ttgagcaagc
      601 ccatgattcc catagctgtc ccttcgatgg atttgagcac agcgtttccc gtgaaaccat
      661 ctgccacaac aacatctgca acgccattca tcaaatcacg cgcttccacg tttccgataa
      721 agttcaaact ttcatcagcc gccagtaatt cataagtttc cttacgaagc gggtcgccct
      781 tgctactctc tgttccgttg ttgagcaaac caacgcgtgg ttgcgcaatg ccacggacat
      841 ttttagcata gaaggaacct aggacagcgt attgatggag gtgctgggct gtattttctg
      901 cattagcacc gaggtcaagc atgtcaaaac ctttcccatc tatagtcggc aatgttgaca
      961 taagtccagg acggtcgata ttcttgatac gacccacgat gaagaatcca gcagccaaca
     1021 aagcacctgt attcccagcc gaaaggacag cgtctgcttc accatctttg acagccttgg
     1081 ctgccaatac catactggca tttttcttat tccgaatagc tctcgtaggt tcatcatccg
     1141 aatcaatctt ctcatccgta tggataatgc cgacgcgctc tgtcgctgtc agatattgct
     1201 tgattttagc ttcatctccg tagagttgaa cctcgatatc tgaaaagtca gatagggctt
     1261 gattgacacc ctcaacgatg gcctgaggtg cgtaatcgcc ccccatggca tctactgcga
     1321 tttttttcat ttcttttcct cttttagtaa ttgtccccag tctgctaggg aatcaataaa
     1381 tttctttgat tttaggtgaa tcccaacgta ctcttcatat agttgatcca taaattggcg
     1441 tagctcttgc ttgattccag gcttgagcga aatggtctcc aaagtctcaa aatcaatagc
     1501 ttgaaattga ttgagcagat aggggatatt gggattgaga tggcaacgtc tctcatcctc
     1561 atgataatgc tctggacaga ggcaggctcc atatttgaaa gaaaagtcaa aagcctgacc
     1621 aacccgatgg cagaagacac actcattaaa attgaggctg attccaaatc gagtcaaaat
     1681 ttgaatttca aaaatattgg tcaaaacctg ataatccaag cctgcttcca tcaactccaa
     1741 agtcttttgc aaaaaagcaa acaagggagc atcctgctga ttgtcctgca aactagcatc
     1801 tgcaagagct gccacatagg tcgcataggc catgacaaag aggtcactat taatcttggg
     1861 aaaagtcatg acctcatgat agtcttcgat gtaactgagt ccgtcatcat tgattcgcaa
     1921 gagaaatcgt gccagcacca agggctgaat aacaggcgcc agcttagact gaccagcgtg
     1981 tttgacaaaa aacatgcgtt tgccaacctg ctctgtaaaa attttgacga gcttatcatc
     2041 ctcacgaaaa ttgcgattgt aaagcaccaa gccttgactc gtgatagact gaatcatgct
     2101 tctctcatgt actcctcaag tcgtttcatg gcttctttga tagtctccat gctggctgca
     2161 taagataggc ggacgtagcc ttccccgtaa cgtccaaagg ctgcaccagg gataaaggca
     2221 acggccttct tctgagcaaa atccttcaga aaagcaaagg agtcttgatt gtagcccgct
     2281 ggaattttag caaaaatata gaaggcaccg tctggtttga taatctcaaa accaagagca
     2341 gtcatttttt cgatgatata gtcccgacgc tggatatatt ccttcttcat gggctccgca
     2401 tcatttttac cagccgtcaa ggcttctacc gcagcatgtt gcgccatggt atttgcggca
     2461 gtgaccaagt attggtgact cttgattaac tgggctgtga aggccgcaga agcgaaaatc
     2521 agccccaaac gccaacctgt catggcatgc gatttagaca aaccattgat aataatagcc
     2581 tggtctctca acatcgttcc tagagacaca tgggcttcgc ctgtgtaggt caattctgag
     2641 taaacctcat cacagacaac aaaaatttcg tacttgcgta aaacagctgc caaggcctct
     2701 acctgctctc gactgtaggt aattcctgtc ggattggctg gatagttgag aataaccgcc
     2761 ttgagcttat caccctgctc caaaatggcc ttctccaaca tctcaggagt caagacaaaa
     2821 ccattttcag tcgtatcaat ctcaacaatt tctgccccaa ctaagttaac aatcggttca
     2881 tagcctggat aagcaggagc tggcaaaagt accttgtctc cctcttccaa aatagccgtc
     2941 aaagtcgcag ataaagcctc tgtcgcccca attgtaacca agatttcatt ttcaggagca
     3001 tagtccagtt ggtacttttc cttaacaaag tcactagctg cctgacgtag agtcagcaga
     3061 ccactcatcc ctgtatagta ggattggttc tgatcaatcg ctcgcttggc cgcctccttg
     3121 acatggtctg gcgttgtaaa atcaggttcc cccaaggtca aacgcaagac cccaggaatc
     3181 tccgaaatag cctggtcaaa ctgacgaatc aacgaaactt gaattttatc taactgttta
     3241 ttaaagcgct tagttaagtc catagatacc tcctactttc aaaacgtatc tctattatac
     3301 tacaaaagcc ccccgaccgc aaaaatacat tagagaatag ctttccactt tctattttac
     3361 ttttcctctt tacaggtcta taatggtaac agattctatt tggaggtttt tatgtcattt
     3421 ctatcaaaaa atggagcagg catcttggtc tgccttctca tttccatcct atcttggtac
     3481 ttaggaggat tcttccctgt gattggcgca cccgtttttg cgatttttat gggcatgctc
     3541 ctacatccct ttctctcgtc ttataaacaa ctggatgctg gattgacctt tagttccaag
     3601 aaattgctcc agtatgccgt tatcttactt ggttttggtc tcaatatctc tcaagtcttc
     3661 gcagttgggc aatcttcact ccctgtcatc ctttccacca tttcaatagc cttgattgtt
     3721 gcctacctct tccagcgctt ctttgccctg gatacaaaac tggctacctt gattggagta
     3781 ggttcttcta tctgtggggg ttctgccatt gcagcgacag cgcctgttat ccatgctaag
     3841 gaaaaggaag tagcccaagc catctccgtt atctttttct tcaatgtctt ggctgcgctc
     3901 atctttccaa ccctcggcac ctggcttcat ctatccaatg acggcttcgc cctctttgca
     3961 ggaactgcgg tcaatgatac ttcctctgta accgccacag ctagcgcttg ggacagtctt
     4021 taccagacca ataccctcga atccgcaacc attgtcaaac tcacgcgcac cctagctatc
     4081 atccccatca ctctctttct ctcctactgg caaagtcgcg agcaaaaaaa taagcaaggc
     4141 ttacaactga aaaaagtctt cccacttttt atccttcact tcatccttgc atcgctccta
     4201 accacccttc tgacctctct cggtgtgtct agtagtttct ttacccctct caaacaactc
     4261 tctaaattcc ttattgtcat ggccatgagt gctatcggtc tcaaaaccaa tctggtcgct
     4321 atggtcaaat ccagtggaaa atccattctt cttggagcca tctgctggat tgccatcatc
     4381 ctcaccactc ttggtatgca gacccttatc ggcattttct aatactcttc gaaaatctct
     4441 tcaaaccacg tcaacgtcgc cttgccgtat atatgtt
//
LOCUS       DBERMR010000089         4390 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.089, whole genome shotgun sequence.
ACCESSION   DBERMR010000089 DBERMR010000000
VERSION     DBERMR010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.089"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            121..1455
                     /gene="pepC"
                     /locus_tag="ACL8A1_002092"
     CDS             121..1455
                     /gene="pepC"
                     /locus_tag="ACL8A1_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HGS1202787.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALECRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
     gene            complement(1511..2422)
                     /locus_tag="ACL8A1_002093"
     CDS             complement(1511..2422)
                     /locus_tag="ACL8A1_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGS1202788.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(2446..3249)
                     /locus_tag="ACL8A1_002094"
     CDS             complement(2446..3249)
                     /locus_tag="ACL8A1_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HGS1202789.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(3277..4266)
                     /locus_tag="ACL8A1_002095"
     CDS             complement(3277..4266)
                     /locus_tag="ACL8A1_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS1202790.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
BASE COUNT         1331 a          923 c          933 g         1203 t
ORIGIN      
        1 agattttcga agagtataaa ttcgaaaaag gtgagggaca atgtcctcgc cttttatgtt
       61 ttttagttgc ttcctttgtg aaaagagtta taatagactg tagaataaaa ggaggaatct
      121 atgaacgcga ttcaagaatc atttactgat aaactatttg ccaactatga agcaaatgta
      181 aaataccaag cgattgaaaa tgctgccagc cacaacggaa tttttgcagc tctggaatgt
      241 cgccaaagcc atgtagacaa cacacctgtt ttctcattgg atttaaccaa ggacaaggtc
      301 actaaccaga aagcgtctgg tcgttgctgg atgtttgcgg ctctcaacac cttccgccac
      361 aaactcatct cgcaatacaa attggagaac tttgagttgt cacaagccca cactttcttc
      421 tgggataagt atgagaaatc aaactggttc ttggagcaag tcattgcgac ttcagaccaa
      481 gaattgacta gccgcaaggt tagcttctta ctccaaacac ctcaacaaga tggcggtcaa
      541 tgggatatgg tcgtttccct ctttgaaaaa tacggtgtcg tgcctaagtc agtttatcct
      601 gagtctgttt catctagcag cagtcgtgag ctaaatgcga tccttaataa attgcttcgt
      661 caagatgctc aaatcttgcg tgatttgctt gtttctggtg cagatcaagc gactgttcaa
      721 gctaagaaag aagacctctt gcaagaaatc tttaactttc ttgctatgtc attaggactt
      781 ccaccacgca agtttgactt tgcttatcgc gataaagata acaactataa aagtgaaaaa
      841 ggaatcacac cacaagagtt ttacaagaaa tatgtcaatc ttcctttaga agactacgtt
      901 tctgttatca atgctccaac tgctgataaa ccttacggaa aatcttacac agttgagatg
      961 ttggggaatg tggttggtag ccgtgcagtt cgctacatca acgttccaat ggagcgcttg
     1021 aaagaattgg cgattgccca aatgcaagca ggtgagactg tttggtttgg ttctgatgtc
     1081 ggccagctca gcaaccgtaa ggctggcatc cttgcgacag atgtttatga ctttgaatca
     1141 agcatggaca ttaaacttac tcaagacaag gctggacgtt tggactatag cgaaagcttg
     1201 atgacccacg ccatggtctt gacaggtgtt gacttggacg aaaatggtaa atcaaccaag
     1261 tggaaggttg aaaactcatg gggagacaag gtcggtacag atggttactt tgttgcctca
     1321 gacgcttgga tggacgaata cacataccaa atcgttgttc gtaaggaatt gctgacagca
     1381 gaagaacaag ctgcctatgg agcagaacca atcgtacttg caccatggga tccaatggga
     1441 gccttggctg aataaaagca tagaaaaaag gaatcagatt ttagaacctg gttccttttt
     1501 agttgcttga ttacatgatg tgaagaacat gtgccacaat acccactgcg aagagggcaa
     1561 ggataatagt gattggagat acttttttct taagtaagta catgcaaagt aaagtaagga
     1621 gtagtcctga taatccagga atcaacatat ccaagttttg ttggaaagta gtaacttttt
     1681 caggagtttg agacaatcct tgtcctactt gtgcgaatgc ttcttggata cctttagacc
     1741 cttctggcaa tttatcccaa tggatataag ccttttcatc tagttgaact ttagaaacat
     1801 cgaaagcaaa tttaatattt acccagcgtt gaacaaggac agcaagaatg aacatcccaa
     1861 ggatagaagc tcctttagtg atatcttgaa ggataccacc agacatatct ttagtgattt
     1921 ctgatccagc cttgtatcca atctcttgaa cataccacaa gaatgacata cgaatcaagt
     1981 tccatgcaac aaagaagagg agtggcccca agatattgcc agtaagggca agtgaagcac
     2041 cgagagatcc aaggattggg cgtactgtaa accagaatac tgggtcaccg ataccagcaa
     2101 gaggtcccat cataccgatt ttaacccctt ggatagcagc gtcatcgatt tccacaccgt
     2161 tagcacgttc ttcttcaagc gcaagagtaa cccccatgac tggagcagct acgtatggat
     2221 gagtgttgaa gaactcaagg tgacgctcaa gagcagcgat ttgatcttct tttttagtat
     2281 agagtttttt gatagctgga atgagtgtat aagcccagcc caagttttgc atccgttcaa
     2341 agttccaaga cccttgtaag aaggttgaac gccaccaaac ttttttacga tctgatttag
     2401 ttaattgaag tttttcagtc atgatgtttt cagtcctttc ttatcttagt agtcttctag
     2461 gatatcgccg attgggtcgt tagaagttgc ggctcctccg ccaccatttc caccagtttt
     2521 agaaaggtga aggtagataa gagcgatagc aacaccgata gcaccgaatc cgattagagt
     2581 aatatctgac acagcagcga gaacgaaacc aagagcgaag aatggccata cttcacgagt
     2641 tgccatcatg ttgataacca tggcgtaacc aacggcaacg accataccac caccgatagc
     2701 cataccatct ttgagccagt ctggcatggc actaaggata ctttgtacag tttcagttgg
     2761 taccataagg agaagagctg caggaagcgc gatacgaagt ccttggaaaa gtagcgcaat
     2821 gaaatgcgca cgctccacag cgccgaagtc acctttttta gcggcagcat ctgcagtatg
     2881 aaccaaacca actgaaattg tacgaacaat cattgtcaag aaaagtccag ctacggcaag
     2941 agggatagca accgcttggg caacaccgat accagtcttg gtaaagtcac caccaagaac
     3001 catgataatg gcagcagcga cagaagcaag tgcagcatca ggagcgatag cagcaccgat
     3061 atttgaccaa ccaagggcaa tcatttgaag cgatccaccg aggataatcc ctgcttccaa
     3121 gtgacctgtt acaagcccaa taagggtaca ggctacaagt ggttggtgaa attggaactg
     3181 gtcgaggatg ccttcaagac ctgcaaagaa ggctacaacg actactaaaa ccatagaaat
     3241 aatagacatg tttaaaatcc tttcataaat aatggcttat ttgacattgg ctttgttaat
     3301 caagtcaaac aaatcttttt tagaatcatt tggtacttta cggacatcaa attcaacacc
     3361 caagtcacgc attttttcaa atgtagcaac atcttctttg tccatagaca aaacggtatt
     3421 gaccaatgtt ttacctgttg agtgagccat agaaccaacg ttaagagtct tgattggcac
     3481 gccgccttcg atggcacgaa gggcatcttg aggtgtttca aacaagataa gggcatgtgt
     3541 ttctccaaaa cgtgggtctt ttgaaatctc aatcagtttt tgaattggaa ccacgttagc
     3601 cttgacatta cctggagctg cttgtttaat caattcttta cgaaggtcgt ctttagccac
     3661 gttatctgaa gcaacgatga tacgatttgc ttttgaatct ggagtccaag cagttgcaac
     3721 ctgaccgtga agtagacgtg tgtcaagacg ggcaagattg attttcaatt taccgtctcc
     3781 gataacagtt ccttctggga tagcagtttg ggcaactgga gcagctgcag cgcttgcaac
     3841 ttcttcaact ggatttagct cttctggaag agctttgatg ccatctttgg cttctttaat
     3901 gatattagca gcgacttttt ctacacctgc agcagcgtcc atgaggcgct ctgtgtaggc
     3961 ttgaatcaac atcggtaagt taagtcctgt gatgatggca aacttacgct caggattttc
     4021 tcccatcacg cgactagctt ggttaaatgg agaaccactc caaaggtcag ccaaaactag
     4081 aacctcatct tctgcgtcaa atgcagcaac agcgttatta aacttagcgt atagatcatc
     4141 aggaccttca tttggcataa aggtcacaac ttgaaccttt tcttgttcac caaagatcat
     4201 agatcctgac tgatgaatac ccgcagcaaa ttcgccgtgg ctcgcaataa tgattccgat
     4261 actcattatt gtcattcctc cttttttgtt ttagttttca tttaagaaaa ctttaagact
     4321 gtttaagtat aaaccgtttt cataaaaaaa gcaactattt atcataaaaa attaaaaaat
     4381 ttttattttt
//
LOCUS       DBERMR010000090         4383 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.090, whole genome shotgun sequence.
ACCESSION   DBERMR010000090 DBERMR010000000
VERSION     DBERMR010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.090"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(159..299)
                     /locus_tag="ACL8A1_002096"
     CDS             complement(159..299)
                     /locus_tag="ACL8A1_002096"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202791.1"
                     /translation="MDRTDEVSSKHRFEVADRTDQVSSKHRFEVADRTDEVSNIYTAR
                     RS"
     gene            655..882
                     /locus_tag="ACL8A1_002097"
     CDS             655..882
                     /locus_tag="ACL8A1_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202792.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            1170..4085
                     /gene="nanA"
                     /locus_tag="ACL8A1_002098"
     CDS             1170..4085
                     /gene="nanA"
                     /locus_tag="ACL8A1_002098"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HGS1202793.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGGGSSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKISRDYYARDLE
                     NVETVIEKEDVETNAANGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVS
                     SATKKDEYFTMAVYNNTATLEGRGSDGKQFYGNYNDAPLKVKPGQWNSVTFTVEKPTP
                     ELPKGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLT
                     VYNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGKPNKDGIKSYRI
                     PALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKAS
                     DPSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQIL
                     YREGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGNQLLGNIYFTT
                     NKTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGP
                     HKGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTM
                     NNRRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYV
                     QMSAIHTMHNGKEYIILSNAGGPNRENGMVHLARVEENGELTWLKHNPIQKGEFAYNS
                     LQELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKDLISPTEAKVKRTREMGKGEM
                     GKGEMGKGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEI
                     IGIAKGSIESMHNLPVNLAGARVPGGVNGNKAAVHEVPEFTGGVNGTEPAVHEIAEYK
                     GSDSLVTLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
BASE COUNT         1466 a          732 c          954 g         1231 t
ORIGIN      
        1 attatattta tagaattttt tgttgctaga tttgtcaaat tgcttaaaat aatttttttc
       61 agaaagcaaa agccgatacc tatcgagtag ggtagttctt gctatcgtca ggcttgtctg
      121 taggtgttaa tacttttcaa aaatctcttc aaaccacgtc agcttcgcct tgccgtatat
      181 atgttactga cttcgtcagt tctatctgcc acttcaaaac ggtgttttga gctgacttgg
      241 tcagttctat ctgccacttc aaaacggtgt tttgagctga cttcgtcagt tctatccaca
      301 acctcaaaac agtgttttga gcatcatgcg gctagtttct tagtttgctc tttgattttc
      361 attgagtata aaaacagatg agtttctgtt ttctttttat ggactataaa tgttcagctg
      421 aaactacttt caaggacatt attatataaa agaatttttt gaaactaaaa tctactatat
      481 tacactatat tgaaagcgtt ttaaaaatga ggtataataa atttactaac gcttataaaa
      541 agtgatagaa tctatcttta tgtatattta aagatagatt gctgtaaaaa tagtagtagc
      601 tatgcgaaat aacagataga gagaagggat tgaagcttag aaaaggggaa taatatgata
      661 tttaaggcat tcaagacaaa aaagcagaga aaaagacaag ttgaactact tttgacagtt
      721 tttttcgaca gttttctgat tgatttattt cttcacttat ttgggattgt cccctttaag
      781 ctggataaga ttctgattgt gagcttgatt atatttccca ttatttctac aagtatttat
      841 gcttatgaaa agctatttga aaaagtgttc gataaggatt gagcaggaag tatggtgtaa
      901 atagcatagg ctgatgtcca tcatttgctt ataaagagat attttagttt aattgcagcg
      961 gtgtcctggt agataaacta gattggcagg agtctgattg gagaaaggag aggggaaatt
     1021 tggcaccaat ttgagatagt ttgtttagtt catttttgtc atttaaatga actgtagtaa
     1081 aagaaagtta ataaaagaca aactaagtgc attttctgga gtaaatgtct tatttcagaa
     1141 atcaggatat agatatagag aggatcagta tgaatcggag tgttcaagaa cgtaagtgtc
     1201 gttatagcat taggaaacta tcggtaggag cggtttctat gattgtagga gcagtggtat
     1261 ttggaacgtc tcctgtttta gctcaagaag gggcaagtga gcaacctctg gcaaatgaaa
     1321 ctcaactttc gggggggggg agctcaaccc taactgatac agaaaagagc cagccttctt
     1381 cagagactga actttctggc aataagcaag aacaagaaag gaaagataag caagaagaaa
     1441 aaatttcaag agattactat gcacgagatt tggaaaatgt cgaaacagtg atagaaaaag
     1501 aagatgttga aaccaatgcg gcaaatggtc agagagttga tttatcaagt gaactagata
     1561 aactaaagaa acttgaaaac gcaacagttc acatggagtt taagccagat gccaaggctc
     1621 cagcattcta taatctcttt tctgtgtcaa gtgctactaa aaaagatgag tacttcacta
     1681 tggcagttta caataatact gctactctag aggggcgtgg ttcggatggg aaacagtttt
     1741 acggtaatta caacgatgca cccttaaaag ttaaaccagg tcagtggaat tctgtgactt
     1801 tcacagttga aaaaccgaca ccagaactac ctaaaggccg agtgcgcctc tacgtaaacg
     1861 gggtattatc tcgaacaagt ctgaaatctg gcaatttcat taaagatatg ccagatgtaa
     1921 cgcatgtaca aatcggagca accaagcgtg ccaacaatac ggtttggggg tcaaatctac
     1981 agattcggaa tctcactgtg tataatcgtg ctttaacacc agaagaggta caaaaacgta
     2041 gtcaactttt taaacgctca gatttagaaa aaaaactacc tgaaggagcg gctttaacag
     2101 agaaaacgga catattcgaa agcgggcgta acggtaaacc aaataaagat ggaatcaaga
     2161 gttatcgtat tccagcactt ctcaagacag ataaaggaac tttgatcgca ggtgcagatg
     2221 aacgccgtct ccattcgagt gactggggtg atatcggtat ggtcatcaga cgtagtgaag
     2281 ataatggtaa aacttggggt gaccgagtaa ccattaccaa cttacgtgac aatccaaaag
     2341 cttctgaccc atcgatcggt tcaccagtga atatcgatat ggtgttggtt caagatcctg
     2401 aaaccaaacg aatcttttct atctatgaca tgttcccaga agggaaggga atctttggaa
     2461 tgtcttcaca aaaagaagaa gcctacaaaa aaatcgatgg aaaaacctat caaatcctct
     2521 atcgtgaagg agaaaaggga gcttatacca ttcgagaaaa tggtactgtc tatacaccag
     2581 atggtaaggc gacagactat cgcgttgttg tagatcctgt taaaccagcc tatagcgaca
     2641 agggtgatct atacaagggt aaccaattac taggcaatat ctacttcaca acaaacaaaa
     2701 cttctccatt tagaattgcc aaggatagct atctatggat gtcctacagt gatgacgacg
     2761 ggaagacatg gtcagcgcct caagatatta ctccgatggt caaagccgat tggatgaaat
     2821 tcttgggtgt aggtcctgga acaggaattg tacttcggaa tgggcctcac aagggacgga
     2881 ttttgatacc agtttatacg actaataatg tatctcactt aaatggctcg caatcttctc
     2941 gtgtcatcta ttcagatgat catggaaaaa cttggcatgc tggagaagcg gtcaacgata
     3001 accgtcaggt agacggtcaa aagatccact cttctacgat gaacaataga cgtgcgcaaa
     3061 atacagaatc aacggtggta caactaaaca atggagatgt taaactcttt atgcgtggtt
     3121 tgactggaga tcttcaggtt gctacaagta aagacggagg agtgacttgg gagaaggata
     3181 tcaaacgtta tccacaggtt aaagatgtct atgttcaaat gtctgctatc catacgatgc
     3241 ataatggaaa agaatacatt atcctcagca atgcaggtgg accgaatcgt gaaaatggga
     3301 tggtccactt ggcacgtgtc gaagaaaatg gtgagttgac ttggctcaaa cacaatccaa
     3361 ttcaaaaagg agagtttgcc tataattcgc tccaagaatt aggaaatggg gagtatggca
     3421 tcttgtatga acatactgaa aaaggacaaa atgcctatac cctatcattt agaaaattta
     3481 attgggaatt tttgagcaaa gatctgattt ctcctaccga agcgaaagtg aagcgaacta
     3541 gagagatggg caaaggagag atgggcaaag gagagatggg caaaggagtt attggcttgg
     3601 agttcgactc agaagtattg gtcaacaagg ctccaaccct tcaattggca aatggtaaaa
     3661 cagcgacttt cctaacccag tatgatagca agaccttgtt gtttgcagta gataaggaag
     3721 atatcggaca ggaaattatt ggtatagcta aaggaagcat cgaaagtatg cataatcttc
     3781 ctgtaaatct agcaggtgcc agagttcctg gcggagtaaa tggtaacaaa gcagcggtgc
     3841 atgaagttcc agaatttaca gggggagtta atggtacaga gccagctgtt catgaaatcg
     3901 cagagtataa gggatctgat tcgcttgtaa ctcttactac aaaagaagat tatacttaca
     3961 aagctcctct tgctcagcag gcacttcctg aaacagggaa caaggagagt gacctcctag
     4021 cttcactagg actaacagct ttcttccttg gtctgtttac gctagggaaa aagagagaac
     4081 aataagagaa gaattctaaa catttgattt tgtaaaaata gaaggagata gcaggttttc
     4141 aagcctgcta tctttttttg atgacattca agctgatacg aaatcataag aggtctgaaa
     4201 ctactttcag agtagtctgt tctataaaat atagtagatt gaaataagat gtgaacaact
     4261 ctatcaggaa agtcaaatta atttatagaa aaattttagc agtcaaggtg tactgttata
     4321 gattcaatat attatgtgac tattaacctt ctcttctcct aaaattgact ttcttgtttt
     4381 ctt
//
LOCUS       DBERMR010000091         4288 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.091, whole genome shotgun sequence.
ACCESSION   DBERMR010000091 DBERMR010000000
VERSION     DBERMR010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.091"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(60..1163)
                     /locus_tag="ACL8A1_002099"
     CDS             complement(60..1163)
                     /locus_tag="ACL8A1_002099"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGS1202794.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQKIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDRLLYASGGARVDGQLLVTRL
                     LEASQVKLVKEKVTLTPLASGYQIGEEEFEQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLVQDMEAYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDETLLQQME
                     EATLTHYLILAEATSKSERVGIRAYTSDFSPFFGQVPDLTGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPENYPIENYVKRVKSE"
     gene            complement(1180..1977)
                     /locus_tag="ACL8A1_002100"
     CDS             complement(1180..1977)
                     /locus_tag="ACL8A1_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HGS1202795.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGAERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWKLEILPSQASTNPFRHI"
     gene            complement(1964..2641)
                     /locus_tag="ACL8A1_002101"
     CDS             complement(1964..2641)
                     /locus_tag="ACL8A1_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HGS1202796.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            3191..3436
                     /locus_tag="ACL8A1_002102"
     CDS             3191..3436
                     /locus_tag="ACL8A1_002102"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202797.1"
                     /translation="MDLLEKECLKCDKNFQQGDIWNYYHLSDKMPAQGWKIHISFQIK
                     DAVDIFKIVYKLSKLNNCSFKVVKNLEELKKLIPLGK"
     gene            3814..4245
                     /locus_tag="ACL8A1_002103"
     CDS             3814..4245
                     /locus_tag="ACL8A1_002103"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HGS1202798.1"
                     /translation="MEKIIKRSNKGNVYRAIRKSDGQKVIIKQSRPFVNYDAEGEWTA
                     LDDIKNEAHMLKKLADKSYTTNLTDEFYIMDDYFLVQEQVDGLNFEEFIRETEHSLNI
                     REKSLDNIVNIVNDIHKLGYKIVDIAPTNFIYTKNVLTPKS"
BASE COUNT         1347 a          901 c          780 g         1260 t
ORIGIN      
        1 cagtgctttg agtaacccgc ggctagttac ctagtttgtt ctttgatttt cattgagtat
       61 tattcgcttt ttactcgttt gacatagttt tcaattgggt agttttcagg gtccaaggtc
      121 aactccttgt cttggatcag ttgagctaaa tggtaaccaa taataggacc agttgtgagg
      181 cctgatgaac ctagtccact ggctgcatag acaccagtta agtcaggcac ctgcccaaag
      241 aaaggagaga aatcactggt gtaggcacgg attccaacac gctcagattt tgaagtagct
      301 tcagccaaaa tcagatagtg agtcaaggtg gcctcctcca tttgttggag caaggtttca
      361 tctaccgtca aatcaaatcc catgtcattt tcgtgggtag cgcctaagga caatttccca
      421 cctgcaaaag gaatcaaatc ccactcccct tctggcatga caacagggta agcttccatg
      481 tcttggacca gctgataatc tcgtagttgt cctttttgag gacgaacatc tacttcataa
      541 cccaagggtt ctaacatgtc ccccaaccaa gctcccgtcg ccaaaataac ctgctcaaac
      601 tcctcttcac caatctggta gcctgatgct aacggtgtca gagtcacttt ttctttgacc
      661 agcttgacct gactggcttc tagcagacga gttaccaaga gttggccatc tactctcgct
      721 ccaccagaag catagagcag gcggtcaaat ccctgcaaac cagggaataa ttcattagct
      781 gaggcttggt ccagaatggc taattgccct atcaagggag attcttctct gcgctggagg
      841 gccaattgat aaagttcttc caacttggat tcatcctttt tcaagagaaa gactcccgaa
      901 cgctggtaaa agtcgatttt ttgtcctgat ttctctaaat cagctaataa atccacataa
      961 aaatcagccc ccaagcgcgc catcttgtac caggctttat tacggcgttt ggaaaaccaa
     1021 ggactgataa ttcctgctgc ggccttggtg gcttgacctt gctcatggtc aaaaacggtc
     1081 acctctaggt cactttctct cgagaggtag taggcagctg ttgctcccac aattcctgct
     1141 ccaataatgg caactttttt cattgtcttc actttctaac tagatatgac ggaaaggatt
     1201 ggtcgatgct tgactaggta aaatctctag tttccagcct tttttctctt tccattgagt
     1261 aaggagtgct gcgatttttt ccacaaaaag cacttcgata tagtgacctg gatccaatgc
     1321 taacaagcca tcagacagca tatcctgagc agtatggtag tagatatcac cagtgatata
     1381 gacatctgcc ccctttgcca aagcatcctt atagaaagac tgcccgcttc caccacaaat
     1441 tgctactctt gaaataggct tctgcaaatc attctcttga taatgcacca ttcgaaggct
     1501 atctaggtca aagacttgct tgacctgttg ggccaattcc caaaatgtct gaggctgaat
     1561 attcccaata cgtccaattc cacgttctgc acctgtttcc tgcagataag tcgtctcctc
     1621 gattcctagc atctgacaaa accagtcatt gagcccattt tcaacgatat caatattggt
     1681 atggctgaca taaactgcga tgtcatgctt aatcaggtcg atgtaaatct gattttgcgg
     1741 acggctggca agcaagtcct tgataggacg aaagataggc gcgtgcttga cgataatcaa
     1801 gtccacaccc ttttcaatgg cctctgccac cgtctcttca cgaatatcga gggcaaccat
     1861 gaccctttgg atacccttgt ctaaagtgcc aatttgcaga ccacggctgt ctccctccat
     1921 agaaaattcc tgagggcaaa aggcttcata agcttggatc acttcacttg ctaacatgga
     1981 gcacctcctt gatagcttga atcttatcta ctagaacttg acgttcttcc agattttttt
     2041 ctgggatttg tccgagggcg aactctagct tctcagcttc tttttgccat ttttggacaa
     2101 atactggact gacttctttg gacaagaagg gaccaaagcg aacatcactg gctgatagct
     2161 tcatttgtcc tgcttccacc accaaaatct cataaaactt tccagcttct tctaagatgc
     2221 tttctgctac aatctggaat ccatgatcct gtagccagat acgcaagtcg tcttcacgat
     2281 tattgggctg gaggatcaaa cgctctacat tagctaactt ccccaaacct tcttctaaaa
     2341 tcctagcaat caaacgacca cccatgccag caatggtaat gacagacact tggtcagtct
     2401 cttcaaaagc tgccaagcca ttggctaaac ggacttggat tttctccttt aggccgtgag
     2461 cctcaacatt tttaaccgca gactgatagg gaccttccac cacctcacct gcaatagcgc
     2521 ttttgatttg gcctctctca accaactcga taggcagata agcatggtca cttcccacat
     2581 ctagtaaaat agccccctgt gacacaaagg aagctaccaa ttctaatctc tttgaaatca
     2641 tcttctctca ctttccaaaa ctctattacc tcttattata ccacatttca atcttcaact
     2701 tcccagtaat ataagcacct ctggcgaaag aagtttcaat gtcctaaagt aataagtgaa
     2761 tccaattgaa agattttaaa caatttgcaa aaatgtcaaa aaataaaaaa taaacagttt
     2821 attcagaaaa atcttgacat ataaaaaaca catggtagaa tataattaga aagttagaaa
     2881 aaataaaagt ttgactaaaa tttgtatttg aaggtggtgt tcagataaga aatttagtca
     2941 gacgaaccac gaatttgctc tatgctttct ggaatttatc ataacaggaa gatacagtca
     3001 tggaacaaac attgtttgaa ttagaactac ttccagagga agatgtcatt gtcacaggtc
     3061 tccctaagta ttgttctttt acttgtttaa ttacaggtcg ctagttatat tttatataaa
     3121 ataagtagct ttacttacgg aataggctag tgctgtgtct ctagcctatt ttaataatta
     3181 ggagtttgtt atggatttat tagagaaaga atgtttaaaa tgtgataaaa atttccaaca
     3241 gggcgatatt tggaattact atcatttgtc agataagatg cctgcacaag gctggaaaat
     3301 acacataagt tttcaaatca aagatgctgt agatattttt aagattgtgt ataaactatc
     3361 caaactaaat aattgtagct ttaaagtcgt taaaaattta gaggaattaa aaaaattaat
     3421 tcccctaggg aaatgagccc tactgctaac aaatttataa ctctatatcc taagtcagaa
     3481 tctgaagcta agagtatgat ttgtaatctt acgaataaac tgtcagaatt taaggctcca
     3541 aaaatactat ctgattttca atgtggacta cattctccag ttcattatag atatggtgct
     3601 ttttttaaaa aaataggctt atgatgaaaa aaataaaaag gtcatctatt tattgctaaa
     3661 tgaaaaaagg aagaactatg tagaagataa gagacaaaat ttccctagtc tccctaactg
     3721 gaaaatggat ttattttcag aagaagaaaa aggaattatt ttcaaaccac atgtgaaatt
     3781 tcaagtaaag actctgcaat aaataaatac aaaatggaaa aaattattaa gagatcaaat
     3841 aaagggaatg tttatcgggc gattagaaaa tcggatgggc aaaaagtaat catcaagcag
     3901 tcaagacctt ttgtgaatta tgatgctgaa ggagaatgga cagctcttga tgatataaaa
     3961 aatgaagctc acatgctaaa aaaattagcc gataaaagtt atactactaa tttaactgat
     4021 gaattttata tcatggatga ttattttctt gttcaggaac aagttgacgg gttaaacttt
     4081 gaagaattta ttcgagaaac agaacactcc ttaaatatta gagaaaaatc actggacaac
     4141 attgtgaata tagtaaatga catccataaa ctgggatata aaattgtaga tatagctcct
     4201 acaaatttta tatacaccaa aaatgtactg accccaaaaa gttagacaaa taatttatcc
     4261 gaaggattta gttctgtatt gcacaagg
//
LOCUS       DBERMR010000092         3961 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.092, whole genome shotgun sequence.
ACCESSION   DBERMR010000092 DBERMR010000000
VERSION     DBERMR010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.092"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..517
                     /locus_tag="ACL8A1_002104"
     CDS             <1..517
                     /locus_tag="ACL8A1_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HGS1202799.1"
                     /translation="LEDLLATVKYYVEHPNERPHSDNGFGNASDHVRKNKADQDSKPD
                     EDKGHDEVSEPTHPESDEKENHAGLNPSADNLYKPSTDTEETEEEAEDTTDEAEIPQV
                     EHSVINAKIAEAEALLEKVTDSSIRQNAVETLTGLKSSLLLGTKDNNTISAEVDSLLA
                     LLKESQPTPIQ"
     gene            727..3846
                     /gene="phtE"
                     /locus_tag="ACL8A1_002105"
     CDS             727..3846
                     /gene="phtE"
                     /locus_tag="ACL8A1_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HGS1202800.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENRKVPTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         1472 a          654 c          775 g         1060 t
ORIGIN      
        1 tcttgaggat cttttggcga ctgtcaagta ctatgtcgaa catccaaacg aacgtccgca
       61 ttcagataat ggttttggta acgctagcga ccatgttcgt aaaaataagg cagaccaaga
      121 tagtaaacct gatgaagata agggacatga tgaagtaagt gagccaactc accctgaatc
      181 tgatgaaaaa gagaatcacg ctggtttaaa tccttcagca gataatcttt ataaaccaag
      241 cactgatacg gaagagacag aggaagaagc tgaagatact acagatgagg ctgaaattcc
      301 tcaagtagag cattctgtta ttaacgctaa gatagcagag gcggaggcct tgctagaaaa
      361 agtaacagat tctagtatta gacaaaatgc tgtggagaca ttgactggtc taaaaagtag
      421 tcttcttctc ggaacgaaag ataataacac tatttcagca gaagtagata gtctcttggc
      481 tttgttaaaa gaaagtcaac cgactcctat acagtagtaa aatgaatgga gcatatttta
      541 tggagaagta acctttcgtg ttacttctct tttttagaaa aacgcaacag aaacttgaca
      601 aaagtaattt taaatagtaa actatttact ggttaattaa atggttaaat aaccggttta
      661 gaaaactatt taataaagta aaagaagttg agaaaaaact tcatcattta ttgaaatgag
      721 ggatttatga aattcagtaa aaaatatata gcagctggat cagctgttat cgtatccttg
      781 agtctatgtg cctatgcact aaaccagcat cgttcgcagg aaaataagga caataatcgt
      841 gtctcttatg tagatggcag ccagtcaagt cagaaaagtg aaaacttgac accagaccag
      901 gttagccaga aagaaggaat tcaggccgag caaattgtaa tcaaaattac agatcagggc
      961 tatgtaacgt cacacggtga ccactatcat tactataatg ggaaagttcc ttatgatgcc
     1021 ctctttagtg aagaactctt gatgaaggat ccaaactatc aacttaaaga cgctgatatt
     1081 gtcaatgaag tcaagggtgg ttatatcatc aaggtcgatg gaaaatatta tgtctacctg
     1141 aaagatgcag ctcatgctga taatgttcga actaaagatg aaatcaatcg tcaaaaacaa
     1201 gaacatgtca aagataatga gaaggttaac tctaatgttg ctgtagcaag gtctcaggga
     1261 cgatatacga caaatgatgg ttatgtcttt aatccagctg atattatcga agatacgggt
     1321 aatgcttata tcgttcctca tggaggtcac tatcactaca ttcccaaaag cgatttatct
     1381 gctagtgaat tagcagcagc taaagcacat ctggctggaa aaaatatgca accgagtcag
     1441 ttaagctatt cttcaacagc tagtgacaat aacacgcaat ctgtagcaaa aggatcaact
     1501 agcaagccag caaataaatc tgaaaatctc cagagtcttt tgaaggaact ctatgattca
     1561 cctagcgccc aacgttacag tgaatcagat ggcctggtct ttgaccctgc taagattatc
     1621 agtcgtacac caaatggagt tgcgattccg catggcgacc attaccactt tattccttac
     1681 agcaagcttt ctgccttaga agaaaagatt gccagaatgg tgcctatcag tggaactggt
     1741 tctacagttt ctacaaatgc aaaacctaat gaagtagtgt ctagtctagg cagtctttca
     1801 agcaatcctt cttctttaac gacaagtaag gagctctctt cagcatctga tggttatatt
     1861 tttaatccaa aagatatcgt tgaagaaacg gctacagctt atattgtaag acatggtgat
     1921 catttccatt acattccaaa atcaaatcaa attgggcaac cgactcttcc aaacaatagt
     1981 ctagcaacac cttctccatc tcttccaatc aatccaggaa cttcacatga gaaacatgaa
     2041 gaagatggat acggatttga tgctaatcgt attatcgctg aagatgaatc aggttttgtc
     2101 atgagtcacg gagaccacaa tcattatttc ttcaagaagg acttgacaga agagcaaatt
     2161 aaggctgcgc aaaaacattt agaggaagtt aaaactagtc ataatggatt agattctttg
     2221 tcatctcatg aacaggatta tccaagtaat gccaaagaaa tgaaagattt agataaaaaa
     2281 atcgaagaaa aaattgctgg cattatgaaa caatatggtg tcaaacgtga aagtattgtc
     2341 gtgaataaag aaaaaaatgc gattatttat ccgcatggag atcaccatca tgcagatccg
     2401 attgatgaac ataaaccggt tggaattggt cattctcaca gtaactatga actgtttaaa
     2461 cccgaagaag gagttgctaa aaaagaaggg aataaagttt atactggaga agaattaacg
     2521 aatgttgtta atttgttaaa aaatagtacg tttaataatc aaaactttac tctagccaat
     2581 ggtcaaaaac gcgtttcttt tagttttccg cctgaattgg agaaaaaatt aggtatcaat
     2641 atgctagtaa aattaataac accagatgga aaagtattgg agaaagtatc tggtaaagta
     2701 tttggagaag gagtagggaa tattgcaaac tttgaattag atcaacctta tttaccagga
     2761 caaacattta agtatactat cgcttcaaaa gattatccag aagtaagtta tgatggtaca
     2821 tttacagttc caacctcttt agcttacaaa atggccagtc aaacgatttt ctatcctttc
     2881 catgcagggg atacttattt aagagtgaac cctcaatttg cagtgcctaa aggaactgat
     2941 gctttagtca gagtgtttga tgaatttcat ggaaatgctt atttagaaaa taactataaa
     3001 gttggtgaaa tcaaattacc gattccgaaa ttaaaccaag gaacaaccag aacggccgga
     3061 aataaaattc ctgtaacctt catggcaaat gcttatttgg acaatcaatc gacttatatt
     3121 gtggaagtac ctatcttgga aaaagaaaat caaactgata aaccaagtat tctaccacaa
     3181 tttaaaagga ataaagcaca agaaaactca aaacttgatg aaaaggtaga agaaccaaag
     3241 actagtgaga aggtagaaaa agaaaaactt tctgaaactg ggaatagtac tagtaattca
     3301 acgttagaag aagttcctac agtggatcct gtacaagaaa aagtagcaaa atttgctgaa
     3361 agttatggga tgaagctaga aaatgtcttg tttaatatgg acggaacaat tgaattatat
     3421 ttaccatcgg gagaagtcat taaaaagaat atggcagatt ttacaggaga agcacctcaa
     3481 ggaaatggtg aaaataaacc atctgaaaat agaaaagtac ctactggaac agttgagaac
     3541 caaccaacag aaaataaacc agcagattct ttaccagagg caccaaacga aaaacctgta
     3601 aaaccagaaa actcaacgga taatggaatg ttgaatccag aagggaatgt ggggagtgac
     3661 cctatgttag atccagcatt agaggaagct ccagcagtag atcctgtaca agaaaaatta
     3721 gaaaaattta cagctagtta cggattaggc ttagatagtg ttatattcaa tatggatgga
     3781 acgattgaat taagattgcc aagtggagaa gtgataaaaa agaatttatc tgatctcata
     3841 gcgtaaggaa tagcagtaga aaaagtctga atcaaaaatg aagttctctc aaaagttaga
     3901 aataaaactc tgactttggg agaatttcat tttattatta atatataaaa tttcttgaca
     3961 t
//
LOCUS       DBERMR010000093         3647 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.093, whole genome shotgun sequence.
ACCESSION   DBERMR010000093 DBERMR010000000
VERSION     DBERMR010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.093"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..132)
                     /locus_tag="ACL8A1_002106"
     CDS             complement(<1..132)
                     /locus_tag="ACL8A1_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGS1202801.1"
                     /translation="MKLTYDDKVQIYELRKQGYSLEKLSNKFEINNSNLRYMIKLIDR
                     "
     gene            412..504
                     /locus_tag="ACL8A1_002107"
                     /pseudo
     CDS             412..504
                     /locus_tag="ACL8A1_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(760..1380)
                     /locus_tag="ACL8A1_002108"
     CDS             complement(760..1380)
                     /locus_tag="ACL8A1_002108"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HGS1202802.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLHTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(1380..1862)
                     /locus_tag="ACL8A1_002109"
     CDS             complement(1380..1862)
                     /locus_tag="ACL8A1_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HGS1202803.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALGLDQMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(1862..2704)
                     /locus_tag="ACL8A1_002110"
     CDS             complement(1862..2704)
                     /locus_tag="ACL8A1_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HGS1202804.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDLKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(2799..3599)
                     /locus_tag="ACL8A1_002111"
     CDS             complement(2799..3599)
                     /locus_tag="ACL8A1_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HGS1202805.1"
                     /translation="MRKQIHPLILFSFLGIMMFILIINRPLNDSQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSAQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
BASE COUNT         1129 a          661 c          723 g         1134 t
ORIGIN      
        1 acgatcaatc aatttaatca tgtacctaag attagaattg tttatctcaa atttatttga
       61 aagtttctct aagctatatc cttgttttct aagttcatag atctgaactt tatcatcata
      121 agttaatttc ataataaaaa caccccaaaa gttagatttt ttctgtctaa cttttggggg
      181 gcagttcatt taacacctga taatatgcgt ttttttattt taaagacttt ttgaccagcc
      241 tcattttttt aacttgatac tcagtgaaaa gccaaaatta aactaggaag ctagctgtag
      301 gctgctcaaa gaacagcttt gaggttgcag ataaaacttg tgaggtcacc aacatatata
      361 atgtgaagct gacatggttt gaatagattt tagaagggta tgagtctgga acttttaatg
      421 gataatgcaa gattccatag aatgggtaag ctagagttct tatgtgaaga gtttgggcat
      481 aaacttttac cttttcctcc ctacttatct tagtatagaa aagtgaatct gaaatagtac
      541 ataactgctt ctaaaacatt cttataatat gatttaaatt ctcaaatcat attattcagt
      601 tcttatttca ttttgctcta caatcctgtt gagaagacac gtgttcatat caaaaaggta
      661 ttggcaagtt gcaatacctt tttacaaggt tcttttgtct tatttttgtt tcaactgact
      721 atatctccta tggttctagt tcagaaggct aggctataat tatgattgat aagaagtatc
      781 attccaagta ttgagagtga atgtttcaaa atcatgggtt tctataatgg tcaggctggc
      841 atttgctaga ccgccatctt tacgaagaag tggttcttta tagcctagga gagtacgaag
      901 actggcagta agattggcgc cgtgtccgac aattagaata cgctcagctg gactatcttt
      961 taatgatttg ataaattgga tggtccgttg agttgtacta tagagggatt cggctccgaa
     1021 cattcgagtg tcaaattgag caagatttga acgaaaagcc tggatttgtt gcgggtaaat
     1081 agcttccaag gttgcaattt tcaaaccttc taacttccca agttgccatt cacggagatt
     1141 aggaacgatt tctaaagaac agggggtatg gagttgactt tggatgatct cagcagattt
     1201 gaccgctcga ggtaaatcac ttgaataaat ctgatcaaaa ggaatttcct tgagatactg
     1261 accaagtcgt tttagggttt caatggattc aggaagaaga ggagaatcac cgctagcacc
     1321 ttgaaaacga ccttcttggt tccagagggt acgaccgtgg cggacaaagt agagtttcat
     1381 tacttgatgt cctccaaaat atctacaaag tctgccttta caaagctagc caagtcttgt
     1441 ggcgcgacga taatgctgtg tccgacttcg cctgcagaga caatcatttg atccaaacct
     1501 agagcaattt tatcgataaa aatgggataa ttgtgtttct gacgaattcc gacaggatta
     1561 ttggctccat gaatgtaacc agttgttttt tctaagtcct tttgtggaat catgctcact
     1621 tttttattgc cagaaatttt agctagtttc ttttcagaca agtgctgagt gatagggaca
     1681 attccgataa tcggtccggt cttgtctccc aaaagcgcca aggttttgaa aatctgatct
     1741 cgttcataac cttgaggaag ctctccttct agggcattga tttgaatccc ctgatgaggg
     1801 atagctgctt tagataggat ttgttctacc aatgtttttt tgattttaac tttttttgcc
     1861 attatttata tttatcctcc aattgactca tccaaatacc aagccagatt cccagcgcaa
     1921 agaagaaggc gatgatgaca taaccgacaa gtgaaagtcc tgtgtattgg atactttcag
     1981 cgtttcctgc atttggaatt aagatcaaaa gggtacttga taggacgata ccgatgatga
     2041 aatgatagac gcgtgagtgg tagttgttta aggcataatc catcaatttt gaaaaaacga
     2101 tgagagttgc acctgcacca attccaatcg gaaagaaggt tcccaagaga tcaaaagttt
     2161 taaaaccagt caacatagga gcatagagtc ccaaaatcaa aagtaaattc gatgggctga
     2221 ggccaggaac caagacgcca agggccaata gtgcgcctgc taggacgaag ttaagaaagc
     2281 tggcgcttaa ggttccaacg acaaaattta aggcatagag tcctaatcca gaaatgataa
     2341 aggttgtcca taaccaagct aaatcaatct tgtctcggtc agattctcga gttgattctt
     2401 tgaggaggct aggaactgta ccgataatag ctcccgcaaa gctccataat acaaagacct
     2461 gataattttc aagcaggtat tcaatcgggt aggaaaataa gccgattccc agaagcatac
     2521 cgatggcaac tggaataaag tacaaaacat tttctttaag gtctttaaag ggatgggcca
     2581 gaaagccaat cattcgttca tagattccta agattgctgc tagaacccct ccggaaattc
     2641 ctggtaggat aaatccaaga gcaattacaa tccctttaat aacgcgtgct aaccatgaga
     2701 gcatatattt cctccaacta tttctatttc aaaaaatcct tactatattg tatcacaatc
     2761 agacacaaaa agaaaaagca aatgataaac aaatgctttt aaggttttaa aaaaagtttt
     2821 cgaaaggttt cttctttatt ttttaaggga gagataacgt tgatatctaa atcgtggtca
     2881 aagccggcaa tttttccttt agatgtgtat tggtgaatat cataatctaa atcagtttta
     2941 ggactgctct ccaaaaatcc tgagtctgag ccgtaggacg gaatccaaac agaggtaaac
     3001 ttgcctgtat caatactgtg ttcttccatg aagtagacac caacgtagat gccgatgttt
     3061 ttagcaccta gtgatgctag ttttgctcga aagttttcga caccttcgtt catattagac
     3121 atggttttgt cttccacgtc aagccaatag taactagggc tgtaaggaga ggcagcattg
     3181 tagaaaactt cggcagcctt ttccatttct tgggcacttt ttccagctac ataagcgtag
     3241 acagcaactg ggacattccg cttttgaagt tcagtgatat gactcttata ggccttgtct
     3301 attccattga taaatgaagc atcattttct tttgtcgttt gagcaccact gtgaacacga
     3361 acaatagcac ctgaaatatt ttgtgagagg gcatcgtagt tgatttcctc aggacgctgc
     3421 cagccagaga ggtcaataat cggtttgtct aagtgtttca aagcctgtgc ttcaatctgt
     3481 gctatattgg attttgtttt aaacgattgg ctgtcattaa gtgggcgatt gatgattaaa
     3541 atgaacatca taatccccaa aaaactaaat aaaataagtg gatgaatttg ttttctcata
     3601 tcttataatt ctaccctaaa aatcaaaaaa aatcaaaaaa atgggtt
//
LOCUS       DBERMR010000094         3097 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.094, whole genome shotgun sequence.
ACCESSION   DBERMR010000094 DBERMR010000000
VERSION     DBERMR010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.094"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..185
                     /locus_tag="ACL8A1_002112"
     CDS             <1..185
                     /locus_tag="ACL8A1_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGS1202806.1"
                     /translation="EYENRTVSFAISSKGEKMKVENISYRVDHRILFDNISFDTSSSG
                     VTLITGKNGTGKSTLL"
     gene            complement(307..473)
                     /locus_tag="ACL8A1_002113"
                     /pseudo
     CDS             complement(307..473)
                     /locus_tag="ACL8A1_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558122.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(551..1861)
                     /locus_tag="ACL8A1_002114"
     CDS             complement(551..1861)
                     /locus_tag="ACL8A1_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HGS1202807.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            2098..2970
                     /gene="nadC"
                     /locus_tag="ACL8A1_002115"
     CDS             2098..2970
                     /gene="nadC"
                     /locus_tag="ACL8A1_002115"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HGS1202808.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT          955 a          517 c          705 g          920 t
ORIGIN      
        1 aagagtatga aaacagaact gtttcttttg ctattagttc aaaaggagaa aaaatgaaag
       61 tagaaaatat ttcgtatagg gtggatcatc gtatattgtt tgataatatt tcttttgata
      121 cttcgagttc aggcgtgaca ttaattactg gtaaaaatgg tacaggaaag tcaactttac
      181 tatagtagat tgaaactaga atagtacaca tctacttcta aaatattgtt agaaatcgat
      241 ttgactatcc tgatctattt gtcctgttct tatttcattt cactatatct caaattgagt
      301 atgacgaagt gcgctcccat gtcctgggaa cgcactttct tcatattttt catattcttg
      361 aatccatcga taaagactat tgggatgaat ttttaaagtt gaactaatca tttttacagg
      421 atgagattta cagcagagag tttgaaggct ttatcaaagg tttttcttgg cataatgact
      481 tttcctcgtt tccacttaat tttgtgtcta ctttattata ccaagtccac ccttaagtta
      541 gataataaat ctaacttaag gaagctagaa ggatgagaat ccaggtggtc aagagtccca
      601 aacttaagct gatggggaca cccagaataa tttgcttttt gaaggcaagg ccacgttcct
      661 ctatattggg aagtgagagt tgaatgagag aaccagctga tgaaaagggt gagatattag
      721 tagatagagc gccaataacg gtggctgttg tgagtaagtg aatatcaatc tgaggatttt
      781 gagcactgat gatagcaatg atgggaaaga gggctggagc tacaacggat agggtggaac
      841 taaagagtga catcactccg gctatcacac aaaagaacag aggtaaccag aaatgaggaa
      901 tggttgttgt catgaggtgc cctatcagtg tgactaaacc tgacttgacc gctagagaca
      961 ttagtaagct catgccgcag agcatgataa ttgtagccca gggaacctta gctaaaatgg
     1021 cttcttgctt ccctaatttg agccttaagg cgaggcagac catgagtatt gagacaaagc
     1081 caatatcaaa tgttttttga taagtagcta tccaggcgat gtttgggaaa atgagatgca
     1141 acaagggaaa aagccaaacc aaaaccatgc tgctgatcat gagcaaggtg gtttgtcttt
     1201 gaaccttgct gaggagtggt ggttggtcaa tagtcaagga tgagtttgtt cttcccttac
     1261 tatagtgact gtaacaggat aataaaagca agacgatgag tgggtagata atgctgacga
     1321 taaagatatg attgccaagt gaaaaagctt gctcttccca tcccatttgc ttaaacaggc
     1381 cttgaaagac aatgcctgag ctactggtta tcaaattagc ccctcctgaa gctccccaat
     1441 tgacggcttg agctccaatc aaagggtgtt tgtccgcttt ttgacagagg gtaatcgcta
     1501 gaggacagca aacggccata gtagtgaaaa atccagcacc taaagcagac aaaagggttg
     1561 ccatcaggta taaaatcatg tagagggcgt tagggtgggt gcgtgtgcgg tagagaatgt
     1621 gttgagccaa aacatcaaga gtaccgttag ttgttgcaac gttataaaag agagagacgc
     1681 taaaaatggt aaaaaagagt gaggttggcc aaaaatgaag aagttctttg gggcttaatc
     1741 ccatgagagt ggttgcgatg aggtaagaaa aagcaatagc cagcaggcca atattgattt
     1801 tggtgcggta accaattcca atggctagag caatggcgct aatcattatt aaatgaatca
     1861 ttgaattgtc ctttcagtta gaatatggaa ataggttggc aattttggcg ctcaaaagac
     1921 cacctgtttc ctattggtac ttgactatta tacacaaaaa aaatacaatt gtcttgacaa
     1981 ttgtattgac acttgttaga attaaaaatg aggtattcag gcttgtgatt tcctatggaa
     2041 gttaatagta attgcctcta gtgcttacaa gtgatattaa aaatagagga cttagtgatg
     2101 tcaatcattt caactgattt aacccctttt caaatagatg atacattgaa agcagccttg
     2161 cgagaagatg ttcattccga agattacagt accaatgcca tttttgatca tcatggccaa
     2221 gccaaggtgt cgctttttgc caaggaagct ggtgttttag cgggactaac cgtttttcaa
     2281 agggttttta ccctatttga tgccgaggtg accttccaga atcctcatca atttaaggat
     2341 ggggatcgtt tgactagtgg cgatttggtt ttagaaatca taggatcggt gagaagtctc
     2401 ttaacatgtg aacgcgttgc cttgaatttt ttacaacatt tatcagggat tgcttcgatg
     2461 acagctgctt atgtagaagc cttaggcgat gattgcatta aggtatttga tactcgaaaa
     2521 actactccta atttacgtct ttttgagaaa tatgccgtga gagttggcgg tggctataat
     2581 catcgcttta atttatcaga tgctatcatg ctaaaagaca atcacattgc ggcagtaggt
     2641 agtgttcaga aggcaattgc tcaagcgcgt gcctatgccc cttttgtgaa aatggtcgag
     2701 gtggaagtgg aaagccttgc tgctgccgaa gaagctgcgg cggcgggtgc tgatattatc
     2761 atgttggata atatgtcatt ggaacagatt gaacaggcca ttaccctaat tgcaggacgt
     2821 tctcggattg aatgttctgg aaatattgat atgaccacta ttagccgttt tcgtggttta
     2881 gcgattgatt acgtctccag tggtagttta acccatagtg ctaagagtct tgatttttcc
     2941 atgaagggtt taacctacct tgatgtctaa gttgtaaaat aaactaactt tttaaaggat
     3001 gtctttcctc tagaacgagt tttatgtcag atagtttaaa cgcctcttca aatatagtaa
     3061 aatgaaccaa aaatagtaca caatgaggta taatctt
//
LOCUS       DBERMR010000095         2982 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.095, whole genome shotgun sequence.
ACCESSION   DBERMR010000095 DBERMR010000000
VERSION     DBERMR010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.095"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(55..534)
                     /locus_tag="ACL8A1_002116"
     CDS             complement(55..534)
                     /locus_tag="ACL8A1_002116"
                     /inference="COORDINATES: protein motif:HMM:NF013842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 transposase-related protein"
                     /protein_id="HGS1202809.1"
                     /translation="MGKERRKQGNLERKKRVVKKRKIPLEELKAFVEAHPDAFLREIA
                     ARFDCALPSVWAVLKQIKVILKKTTSFREQKPEKVSEFLDILDNLKDLPIVYIDEMGI
                     NRYLYRPYAGAPRGEKVYDKISGRRFERTNEVEQKLNGSFLIRYIDSQIREWQNNYA"
     gene            complement(501..602)
                     /locus_tag="ACL8A1_002117"
     CDS             complement(501..602)
                     /locus_tag="ACL8A1_002117"
                     /inference="COORDINATES: protein motif:HMM:NF013842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202810.1"
                     /translation="MKGGHRHVEAAKVFDVGARTLFTWEKKDVNKGT"
     gene            911..1291
                     /locus_tag="ACL8A1_002118"
     CDS             911..1291
                     /locus_tag="ACL8A1_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HGS1202811.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            1390..1611
                     /locus_tag="ACL8A1_002119"
     CDS             1390..1611
                     /locus_tag="ACL8A1_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HGS1202812.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            1628..1942
                     /gene="trxA"
                     /locus_tag="ACL8A1_002120"
     CDS             1628..1942
                     /gene="trxA"
                     /locus_tag="ACL8A1_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HGS1202813.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            2248..2781
                     /gene="queF"
                     /locus_tag="ACL8A1_002121"
     CDS             2248..2781
                     /gene="queF"
                     /locus_tag="ACL8A1_002121"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HGS1202814.1"
                     /translation="MKRQLALVVFSGGQDSTTCLFWVMQHYETVEAVTFAYGQRHHLE
                     IQITREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEAWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
BASE COUNT          936 a          620 c          564 g          862 t
ORIGIN      
        1 taaagacacg caaatgagta taaatcagct aatttttggt ttatattcct atttttatgc
       61 ataattattc tgccattctc taatttgtga atcaatatat ctgattagaa aactaccatt
      121 gagtttttgc tccacctcat tagtccgctc aaaacgccgt ccgctaatct tgtcatagac
      181 tttctcccct ctaggagccc ctgcataagg acgatagagg tagcggttga ttcccatttc
      241 gtcaatatat acgattggta aatcttttag gttatccaaa atatcaagaa actcagaaac
      301 tttctcaggc ttttgttccc taaaactggt cgtctttttt aaaatgacct taatctgctt
      361 taaaactgcc catacggagg gcaaagcaca atcaaaacgg gccgcaattt cccgtaaaaa
      421 agcgtctgga tgagcctcta caaaggcttt caattcttct aaagggatct tacgcttttt
      481 gacgactcgc tttttccgct ctaagttccc ttgtttacgt ctttcttttc ccacgtgaag
      541 agagttctgg cgccgacatc aaaaactttg gccgcctcga catgtctgtg ccctccttta
      601 atggaatcta atgctccttg tttaaaatca gtactatatg catagcttta ttagaccatg
      661 cttattttaa actaagcaac tatagataga gaaatgaaat aaaatctgaa ttcgaatgtg
      721 ataaggagtt agaaattgaa acaataggat tcccccatta ttcacacaaa ataaaaccac
      781 taaaaaacag agcttgccag cattcttgaa aagtagtata attattgcat gcgcaatcat
      841 cttgtgacgc agagactgcc cgttaatgga ctttcttcta tattatgact ctaaaaagaa
      901 aggagacact atgacctatt tggaaaaatg gtttgacttc aatcggcgtc agaaagaaat
      961 tgaaagtctc ttggaagaga ccattgccca acagagcgag caaagtctga ccttgaaaga
     1021 gttctacctg ctctactatc tggacttggc tgaagaaaaa tctctacgcc agattgacct
     1081 gccagataag cttcatctga gcccgagcgc agtttctagg atggtggctc gcttggaagc
     1141 taaaaattgc ggtctactca gtcgcatgtg ttgccatcaa gatagacgct ctagttttat
     1201 ctgcctgaca aatgatgggc aaaagacact ggcctcgcta caaaagactg tcgaagaaag
     1261 cttggaagca ggtttggatt tcttgattta agatgatttt aaaaaaagtt gcgtgcgcaa
     1321 tcatttttct tgacattctc ttttataagg agtaaaataa agttagcatt aaacaaagga
     1381 gttttaaata tgattgaaat tacctatata gatgccagca agaacgaaag aactgtgact
     1441 ttcgagtctt atgaagactt tgaacgttcg caacaagctt gccttatcgg cgtcgcagac
     1501 tactaccctg tccaaaaatt aacttacaag ggtcataatt tggactacca tgggacttat
     1561 ggagatatct tcttctatct catgaaacaa gatttaagcc aatataacta aaaaaaggag
     1621 aaatacaatg gcaaaagcaa ttacagatgc aacattcgaa caagaaacaa aagacggttt
     1681 ggtcttagta gacttctggg caacttggtg tggtccatgt cgtatgcaag gtccaatctt
     1741 ggacaaattg tctgaagaac tttcagaaga tgtcttgaaa atcgttaaaa tggacgttga
     1801 tgaaaatcca aacacagctc gtgcttttgg aatcatgtct attccaactc ttctcttcaa
     1861 aaaagacggc caagttgtca aacaagttgc aggtgttcac acagcagaac aaatcaaggc
     1921 catcattgct gaattgagct aatcaaacga gagaccaagt actttctttg gtctcttttt
     1981 tcttgccctt tgccattttt caaaaaatat gctagactat agtagattga aactagaata
     2041 gtacacctct acttctaaaa cattgttaga aatcgatttg actgtcctga tcgatttgtc
     2101 ctgttcttat ttcattttac tatataagta gaatattacg atgtttggga tatgccatcg
     2161 ctaaaaaaac ctcgatttgg tattttttag ctccctaatg ggagcttttt gcgtgctctg
     2221 aacatttccc attttggaag gagtactatg aaacgtcaat tagccttggt cgtctttagt
     2281 ggtggtcaag attcaacaac ctgccttttc tgggtcatgc aacactatga aacagtcgaa
     2341 gctgtcacct ttgcctacgg ccaacgtcat cacctcgaaa ttcaaattac tagagaaatc
     2401 gctaaggaac agggcattcg tcaccatatc ctcgatatgt ctctgctggg acaaatcact
     2461 gctcagccag actttgcgac gattcatatt tcctacattc ctgacaagct ctgtgtcgag
     2521 tcaaaatccc tcaaactata tctatttagc taccgaaacc acggagattt ccacgaaaac
     2581 tgtatcaaca ccatcgggaa agacttggtc aacttgctag accctcgcta tttagaagcc
     2641 tggggaaaat tcactccgcg cggtggcatt tcaatcgacc cctactataa ctacggtaag
     2701 caaggaacta agtatgaggg cttggcagaa caacgcctct tccaacacga cctttatcca
     2761 gagaaaattg acaaccgcta aagtcatact caatgaaaat caaagagcaa actaggaagt
     2821 tagccgcagg ttactcaaag cagtgtttga ggttgtagat aaaactgacg aagtcagatc
     2881 aaaacactgt tttgaggtgg cagatagaac tgacgaagtc agatcaaaac actgtttgga
     2941 ggttgcagat agaactgacg aagtcagatc aaaacactgt tt
//
LOCUS       DBERMR010000096         2848 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.096, whole genome shotgun sequence.
ACCESSION   DBERMR010000096 DBERMR010000000
VERSION     DBERMR010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.096"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            327..1673
                     /gene="gdhA"
                     /locus_tag="ACL8A1_002122"
     CDS             327..1673
                     /gene="gdhA"
                     /locus_tag="ACL8A1_002122"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HGS1202815.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
     gene            complement(2179..2349)
                     /locus_tag="ACL8A1_002123"
     CDS             complement(2179..2349)
                     /locus_tag="ACL8A1_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202816.1"
                     /translation="MKKENEYVILTIASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
     gene            complement(2362..>2848)
                     /locus_tag="ACL8A1_002124"
     CDS             complement(2362..>2848)
                     /locus_tag="ACL8A1_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS1202817.1"
                     /translation="SQNLGVVPQDSFLLNRSILDNITLKHEVTSQKIEEVCKAVQIYD
                     EIMAMPMKFNTIISEMGSNISGGQRQRIALARALINNPSIVILDEATSALDTINEERI
                     TKYIQSQGCTQIIIAHRLSTIKDADIIFVMKGGKIVESGNHKYLITLGGEYYSLYTKR
                     K"
BASE COUNT          904 a          534 c          481 g          929 t
ORIGIN      
        1 tataatactt ttttatttta gtctagtata aatattgctt tcctccaaaa tggtcatagt
       61 tttactggca aatctaacat atcacggata aattaacaag tgatttctga attgctaaac
      121 attttctttt cttatagcat actttaagat tttgtctttg agaaagatat ttccaagaaa
      181 aacgttcgtt tttttatcta ttttcaaaaa aacccaaaaa ctgaattgaa agaattttta
      241 gaaaatttct gttttttctt tacagtgaaa aaaaattctg ctataatgtc ttatgtaata
      301 aaatatgaca ataaaaggag aacgatatga catctgctaa agaatatatc caaagcgtgt
      361 ttgaaactgt gaaagctcgt aacgggcacg aggctgaatt cctccaagct gttgaagaat
      421 ttttcaacac tttagaacct gtatttgaaa aacaccctga gtatatcgaa gaaaatatct
      481 tggcacgtat tactgagcct gagcgcgtgg tttctttccg tgttccttgg gttgaccgtg
      541 atggaaaaat tcaagtaaac cgtggttacc gtgttcaatt caactcagct gttggaccat
      601 acaaaggtgg acttcgtttc cacccaactg taaaccaagg gatcttgaaa ttcctcggat
      661 ttgaacaaat ctttaaaaat gtcttgactg gacttcctat cggtggaggt aaaggtggat
      721 cagacttcga tcctaaaggt aaaacagatg ctgaagtgat gcgcttctgc caaagcttca
      781 tgactgaatt gcaaaaacac atcggaccat cacttgacgt acctgctggt gatatcggtg
      841 ttggtggacg tgaaattggt tacctttacg gtcaatacaa acgtcttaac caatttgatg
      901 ctggtgtctt gactggtaaa cctcttggat ttggtggtag cttgattcgt ccagaagcaa
      961 ctggttacgg tttggtttac tatactgaag aaatgctcaa agctaacggt aacagctttg
     1021 ctggtaagaa agtcgttatt tcaggttctg gtaacgttgc tcaatatgct cttcaaaaag
     1081 caactgaact cggtgcaact gttatttctg tttctgactc aaatggttat gtcatcgatg
     1141 aaaatggtat cgacttcgat cttttggttg atgttaaaga aaaacgtcgt gctcgtttga
     1201 ctgagtatgc agctgaaaaa gcaactgcaa cctaccacga aggtactgta tggacttacg
     1261 ctggaaacta tgacattgct cttccatgtg ccactcaaaa cgaaatcaac ggtgaagcag
     1321 ctaaacgttt ggttgctcaa ggcgttatct gtgtatctga aggtgccaac atgccgagcg
     1381 accttgatgc catcaaagtt tacaaagaaa atggtatctt ctacggacct gcaaaagctg
     1441 ccaacgctgg tggtgtagcc gtttcagctc ttgaaatgag ccaaaacagt cttcgcctct
     1501 catggactcg tgaagaagtt gatggccgtc tcaaagacat catgacaaac atcttcaaca
     1561 cagctaaaac gacttcagaa acatacggtc ttgataaaga ctaccttgca ggagctaaca
     1621 ttgctgcctt tgaaaatgta gcaaatgcca tgattgcaca aggtattgtt taagatttat
     1681 ttactcaatc ctcaatcact acgattgggg atttttagct tgaattaaaa aattggaaaa
     1741 tctaattaaa taaaatgaaa tgaaataaaa actgaacaaa ttgattgcgg aattcaaacc
     1801 aatttctaac atacttctag aaattaattt gacttttcta atcgatttgt tcatatctta
     1861 tttcaattta ctatacttac ttaacccttt ttatactcaa tgaaaatcaa agagcaaact
     1921 aggaagcgag ccgcaggctg tacttgagta cggcaaggca aagctgacgt ggtttgaaga
     1981 gattttcaaa gagtattaaa caaaaagaaa aaccgctact cctaagaata gcgatttaat
     2041 catgttttta agctactact gtagttgctc tattatttaa gtgttatgaa taacttcaat
     2101 atctttaaag ctttttgtta gatttataat ttcaactata gccatctcta ctccttagtt
     2161 tacagcaaaa aattatgctt aattcttatt gtttttgtaa acaatcagcg aacccaatac
     2221 tattccattc aacaagccta aagaaatacc atattcaact ggaaaatcta aaaaaattgc
     2281 aaacactatt ccaatcatca cccctagtga ggctattgtt aaaattacat attcattttc
     2341 tttcttcatt ttctttacac ctcatttcct ttttgtatat aagctgtagt actctccacc
     2401 aagagttatt aagtacttat gatttcctga ctcaacaatc ttaccacctt tcattacaaa
     2461 aataatatcc gcatccttaa tcgttgacaa tctatgagct ataattattt gagtacaacc
     2521 ctgactttgt atatactttg ttattctttc ctcattaata gtgtctaatg cactagttgc
     2581 ttcatctaaa attacaatac taggattatt tattaatgca cgtgccagtg ctatccgttg
     2641 cctttgccca cctgaaatat ttgaccccat ctctgagatg atagtattaa atttcatcgg
     2701 catagccatg atttcatcat agatttgaac tgctttacaa acttcctcta tcttttgtga
     2761 agtaacttcg tgctttaaag ttatattatc aagaatactt cggttcaata aaaatgaatc
     2821 ctgtggaact actcctaaat tttgactt
//
LOCUS       DBERMR010000097         2627 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.097, whole genome shotgun sequence.
ACCESSION   DBERMR010000097 DBERMR010000000
VERSION     DBERMR010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.097"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(388..1281)
                     /locus_tag="ACL8A1_002125"
     CDS             complement(388..1281)
                     /locus_tag="ACL8A1_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS1202818.1"
                     /translation="MDELKREIGYKIKTIRRSCGKTQIDICGDESELTIRQLARIENG
                     QALATLPKLLLIADKLGVSLQNIVDVKVIEIPKGFLKLKDELIHSQTYADKGRIERKE
                     AILEEIYENYYENLPEEEQLIVDVTQARFDIYGSSDVTYGLGLVEEYFQQLLKKKYFS
                     VNDLLIIELYFFCCAMGLEDKEHFEELAQKVLLCSEYEDKDSLVQMEKVLLSLFIQIQ
                     TEDSLIYIQTFEKIIAKTRHVFYRPHLFLLKAKYALFVDKNVAEAESFYEKAISLAEL
                     LDDQVLVQKILAEKQIDFPTT"
     gene            complement(1302..1496)
                     /locus_tag="ACL8A1_002126"
     CDS             complement(1302..1496)
                     /locus_tag="ACL8A1_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202819.1"
                     /translation="MKKVLKYFLVFVFLFLMNIFIFKILATLGFQLIMSEKSYIVLPL
                     FSLIVLYMIDKRIRKKKKSL"
     gene            complement(1907..2032)
                     /locus_tag="ACL8A1_002127"
     CDS             complement(1907..2032)
                     /locus_tag="ACL8A1_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PncF family bacteriocin immunity protein"
                     /protein_id="HGS1202820.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKKFLKSSEKTTEKSVKK"
     gene            complement(2035..2265)
                     /gene="blpU"
                     /locus_tag="ACL8A1_002128"
     CDS             complement(2035..2265)
                     /gene="blpU"
                     /locus_tag="ACL8A1_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HGS1202821.1"
                     /translation="MNTKKMSQFEIMDTEILACVEGGGCNWGDFAKAGVGGGAARGLQ
                     LGIKTRTWQGAATGAAGGAILGGVAYAATCWW"
     gene            complement(2468..>2627)
                     /locus_tag="ACL8A1_002129"
     CDS             complement(2468..>2627)
                     /locus_tag="ACL8A1_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HGS1202822.1"
                     /translation="NHALSKESSKVENIFAKVKTFKMFSTTYRNHRKRFGLRMNLIAG
                     IINHELGF"
BASE COUNT          868 a          543 c          289 g          927 t
ORIGIN      
        1 tcaaattact tttatatcac taagactcaa taaaagataa tgtctattta acttttatac
       61 gattactacc tcctttttag tttacacttc ctacatcata aataaacata ctcaaccctc
      121 acaatctcca aataactctt ctatatcatt gaaaattgag tttcatcctc tatactaatg
      181 taggaaacct attaaaacat tcctttaatc caatatagga ataccttatg ccattaatct
      241 ctacctgata ttatagatag aaccagactc ctatacttaa aaggacaaac aacgttccaa
      301 ccataataaa cttcctttct ttttggaatc gttttcttta tgagtttagt ataacaaaaa
      361 catgaaaaaa acaacatgac ttagaagtca ggttgttggg aaatctattt gtttttctgc
      421 taaaattttc tgaactaata cctgatcatc tagcaattct gctagagaaa tcgccttttc
      481 atagaaactt tctgcctctg ccacattctt atctacaaat aaagcatatt tcgcctttaa
      541 caaaaataga tgaggtcggt aaaaaacatg tcttgtttta gcaataattt tctcaaatgt
      601 ttgaatatat atcagactat cttctgtctg aatttggata aataggctca gaagaacttt
      661 ttccatctga actaggctat ccttatcttc atattctgaa catagcaata ctttttgagc
      721 taactcctca aaatgttctt tatcttctaa tcccatagca caacaaaaga aatacaattc
      781 aataatcaac aaatcattta cagaaaaata tttctttttt aataactgct ggaaatattc
      841 ttccactaac cctaaaccgt aggtcacatc tgaactacca taaatatcaa aacgagcttg
      901 tgtcacatcc acaattaact gctcttcctc aggcaaattc tcataatagt tttcatatat
      961 ttcctcaagt atagcttctt ttcgttcgat tcttccctta tcagcgtagg tttgagaatg
     1021 aatcagctca tctttgagtt ttaggaatcc ttttggaatc tctataacct taacatccac
     1081 aatattctgt aatgatactc caagtttatc cgcaatcaat aataatttag gaagtgttgc
     1141 taaagcttgc ccattttcaa tacgtgctag ctgacgaatc gtcaactctg attcatcacc
     1201 acaaatatct atctgagttt ttccacaaga tcgcctaata gttttgattt tataaccaat
     1261 ttcacgtttc aactcatcca tagtaatact ccttaagttg attacaaaga tttctttttc
     1321 ttcctaattc ttttgtcaat catatataaa acgataagcg aaaacagcgg tagaacaata
     1381 taactctttt cactcattat cagctgaaat cccagggttg ctagtatctt aaagatgaaa
     1441 atattcatca aaaatagaaa aacaaatact aaaaaatatt ttaaaacctt cttcatttct
     1501 tttcttcaac tttcctaatt tctacaccta ttttatcgaa aatatatata aaactccaaa
     1561 taaacacttc aactgcactt tttttggcaa aaaatagaca gaacaaacat cctacctact
     1621 aaaaatatga tatgaaagga aaatgatttt ttactgacac cctaggcaag cttgggacaa
     1681 ataaatcatt gatagcaaca tttacgacat cctatttaaa gttccttcaa tttctaatgt
     1741 ccccgctatt tctaagccac ctctggtatg atctattcct agtcagaata gataacaccg
     1801 ttctaaatta cgattcatag ttactccttt tttgactcgt tactataaga ctcctattta
     1861 catgcgcaaa ataattttga ttcagattca tcatttatag ttttgattat tttttaacag
     1921 atttttctgt tgtcttctcc gaagacttta aaaatttttt tctaattaaa tcatccatat
     1981 aaggaccaaa tataccaact actaaaccaa taataaaact tttaaaatcc ataattacca
     2041 ccaacatgtc gctgcatagg ccacacctcc aagtatagct cctcccgcag caccagttgc
     2101 tgcaccttgc catgttcttg ttttaattcc tagctgaaga cctcgtgctg ctcctcctcc
     2161 aacacctgct ttggcaaaat ctccccaatt gcatccgcca ccttcaacgc aagcaagtat
     2221 ctcagtatcc ataatttcaa attgtgacat cttttttgta ttcataacga atacctcttt
     2281 tttattttta atatttgtct tgttacaaac ttgacaagtt tagtataaca gtatttatta
     2341 atttttttca tccaaatctt gaattggcat cgaaacgtct tgaattagct tttttgtttc
     2401 aaaatcatct ctatttttaa aaaagatgtt ttctaatcac ttttttacta ttagacttcc
     2461 tgcaaaacta gaatcctagt tcatgattga taataccagc aatcaaattc attcgtaatc
     2521 cgaaacgttt acgatgattt cgataggttg ttgaaaacat tttaaacgtt tttactttgg
     2581 caaagatatt ctcaaccttg ctgctctcct tagatagcgc atgatta
//
LOCUS       DBERMR010000098         2373 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.098, whole genome shotgun sequence.
ACCESSION   DBERMR010000098 DBERMR010000000
VERSION     DBERMR010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.098"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            8..1624
                     /locus_tag="ACL8A1_002130"
     CDS             8..1624
                     /locus_tag="ACL8A1_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS1202823.1"
                     /translation="MYTIIKSNIKKFSLLTIFIVAGQLLLIYAATINALVLNELIAMN
                     LERFLKLSIYQMIVWCGIIFFDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHS
                     KSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLM
                     IMLLVPKIFASKMREVSLNLTNQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAG
                     ILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTG
                     VIFTALGELGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYEAKNIC
                     YKYGDKEILKNLNFCFQRNEKYLILGESGSGKSTLLKLLNGFLRDYSGELRFCGDDIK
                     KTSYLNMVSNVLYVDQKAYLFEGTIRDNILLEENYTDEEILQSLEQVGLSVKDFPNNI
                     LDYYVGDDGRLLSGGQKQKITLARGLIRNKKIVLIDEGTSAIDRRTSLAIERKILDRE
                     DLTVIIVTHAPHPELKQYFTKIYQFPKDFI"
     gene            1644..>2373
                     /locus_tag="ACL8A1_002131"
     CDS             1644..>2373
                     /locus_tag="ACL8A1_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202824.1"
                     /translation="MRFYCEAYEVITEEKKVYFNDIELEVKYSSVEELFIICEKLLDK
                     KKVSFFYVDEKPLRYLLFDYIFLLVLAKKNIPILDGVSNKQVDPTLLHHFSLEIEKNF
                     IDFCYKNMDLILKTQSISLCHREELIIVDVSDDSKFGVFYKRFRTLVDKSNGKYVCVY
                     NFSRVSDILQNWKNYCNRKFSVTFTESQFELFKLLYNQKNFKTISLLFGKKIVAGGII
                     YYSDLTNIEYFCIFWWDSMFGKDSI"
BASE COUNT          831 a          270 c          429 g          843 t
ORIGIN      
        1 aactattgtg tatactatta taaaatcaaa tataaaaaaa tttagtttat taacgatatt
       61 tattgttgct ggtcaattat tgctaattta tgcagcaact attaatgctc tggtgttgaa
      121 tgaattaatt gcgatgaatt tagagcggtt tttgaaattg tcaatctacc aaatgattgt
      181 ctggtgtggg ataatattct ttgactgggt agtgaaaaat tatcaggttg aagtgatcca
      241 agagtttaat ctagagattc gaaatagagt tgccacagac atctctaact ctacctatca
      301 agaatttcat agtaaatcat caggaacata tctttcgtgg ctaaataatg atgttcagac
      361 tttaaatgat caggctttta aacaactttt tttagtaata aaaggaattt ctggtactat
      421 atttgcagtt gtgactctta atcactatca ttggtcattg actgtagcca ccttgttttc
      481 attaatgatt atgctacttg taccaaaaat ctttgcatcg aaaatgcgag aagttagtct
      541 aaatttaact aaccaaaatg aagctttttt aaaatctagt gagactatat tgaatggatt
      601 tgatgtgtta gcgtccttga atcttttata tgtattgcct aagaaaatta aagaagcagg
      661 aattttatta aagatggtta tacaaagaaa gacaactgta gaaacgttag caggcgctat
      721 tagcttcttt ctcaatattt tttttcagat atctctcgtt tttttaacag gctatcttgc
      781 aataaaagga atagtgaaaa ttggtactat tgaagcaata ggagcactaa caggtgttat
      841 ttttacagcg ctaggtgaat taggaggtca attatcctct attattggta cgaagcctat
      901 ttttttaaaa ttgtattcaa ttaatccaat tgagtcaaat aaaatgaatg atatcgaacc
      961 aaatgaggtg aatagagatt ttccgttata tgaagcaaaa aatatttgct ataagtatgg
     1021 agataaagaa atattaaaaa acttaaattt ttgttttcaa cgtaatgaaa agtatttaat
     1081 tttaggtgaa agtggaagcg gaaaatctac attattaaaa ttattgaatg gctttttgag
     1141 agattatagt ggagaattgc gattctgcgg ggatgatata aaaaaaacct cctatttaaa
     1201 tatggtttcg aatgttctat atgtagatca aaaagcttat ttgtttgaag gtacgattag
     1261 agataatatt ttattggaag aaaattatac tgatgaagaa atactacagt ctttagagca
     1321 agttggtttg agtgtaaaag attttcctaa taacatttta gattattatg ttggtgatga
     1381 tgggagatta ctgtcaggag ggcagaaaca aaaaattact ttagctagag ggctaattag
     1441 aaataagaaa atagtattaa ttgacgaggg aacttctgct atcgatagga gaacttcgtt
     1501 agcgattgaa cgtaagatat tagatagaga ggatttgact gtcattattg ttacccatgc
     1561 tccgcatccg gaacttaaac aatattttac taagatatat caatttccaa aggattttat
     1621 ttaagaatat aggggaggga aacatgagat tctattgtga agcttatgag gtaattactg
     1681 aagagaaaaa agtatacttt aatgatattg agttagaagt taagtattcc tctgtcgaag
     1741 aattgtttat aatatgtgag aaattattag ataagaaaaa agtatcattt ttttatgtag
     1801 atgagaaacc cttaaggtat ctgttatttg actatatatt tctactagtt ctagctaaaa
     1861 aaaatatccc aatcttagat ggagtttcca ataagcaagt tgatccaacg ttgctgcatc
     1921 acttttcttt ggaaattgaa aagaatttta tcgatttttg ctataaaaat atggatctca
     1981 tcctaaaaac tcaaagtata tctttgtgtc atagagaaga gcttattatt gtagatgttt
     2041 cagatgactc taaatttggt gttttttaca agcgttttag aacactggtt gataaaagta
     2101 acggaaaata tgtttgtgtt tataatttca gtcgtgtttc agatatactg caaaactgga
     2161 aaaattattg caatcgaaaa ttttcggtta cgtttactga aagccaattt gaattattta
     2221 agcttttata taatcaaaaa aattttaaaa caatttcgtt gctatttgga aaaaaaattg
     2281 ttgctggagg aattatctat tatagcgatc ttactaatat agaatacttt tgtatttttt
     2341 ggtgggatag tatgtttggg aaagattcta ttg
//
LOCUS       DBERMR010000099         2335 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.099, whole genome shotgun sequence.
ACCESSION   DBERMR010000099 DBERMR010000000
VERSION     DBERMR010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.099"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            144..1340
                     /gene="tuf"
                     /locus_tag="ACL8A1_002132"
     CDS             144..1340
                     /gene="tuf"
                     /locus_tag="ACL8A1_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HGS1202825.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(1449..2335)
                     /locus_tag="ACL8A1_002133"
                     /pseudo
     CDS             complement(1449..2335)
                     /locus_tag="ACL8A1_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          725 a          463 c          483 g          664 t
ORIGIN      
        1 ttaaattttt accaagaata attcacaaaa acgttgtaaa acacttgcaa tttagctgaa
       61 atttgataaa atagtaagga aagttagact gtattgccta ctgtctatct ataaaatata
      121 ttttattgga ggcttttact caaatggcaa aagaaaaata cgatcgtagt aaaccacacg
      181 ttaacattgg tactatcgga cacgttgacc acggtaaaac taccctaact gcagctatca
      241 caactgtttt ggcacgtcgc ttgccttcat cagttaacca acctaaagac tatgcgtcta
      301 tcgatgctgc tccagaagaa cgcgaacgcg gtatcactat caacactgcg cacgttgagt
      361 acgaaactga aaaacgtcac tacgctcaca tcgacgctcc aggacacgcg gactacgtta
      421 aaaacatgat cactggtgct gctcaaatgg acggagctat ccttgtagta gcttcaactg
      481 acggaccaat gccacaaact cgtgagcaca tccttctttc acgtcaggtt ggtgttaaac
      541 accttatcgt cttcatgaac aaagttgact tggttgacga cgaagaattg cttgaattgg
      601 ttgaaatgga aatccgtgac ctattgtcag aatacgactt cccaggtgac gatcttccag
      661 ttatccaagg ttcagcactt aaagctcttg aaggtgactc taaatacgaa gacatcgtta
      721 tggaattgat gaacacagtt gatgagtata tcccagaacc agaacgtgac actgacaaac
      781 cattgcttct tccagtcgag gacgtattct caatcactgg acgtggtaca gttgcttcag
      841 gacgtatcga ccgtggtatc gttaaagtca acgacgaaat cgaaatcgtt ggtatcaaag
      901 aagaaactca aaaagcagtt gttactggtg ttgaaatgtt ccgtaaacaa cttgacgaag
      961 gtcttgctgg agataacgta ggtgtccttc ttcgtggtgt tcaacgtgat gaaatcgaac
     1021 gtggacaagt tatcgctaaa ccaggttcaa tcaacccaca cactaaattc aaaggtgaag
     1081 tctacatcct tactaaagaa gaaggtggac gtcacactcc attcttcaac aactaccgtc
     1141 cacaattcta cttccgtact actgacgtta caggttcaat cgaacttcca gcaggtactg
     1201 aaatggtaat gcctggtgat aacgtgacaa tcgacgttga gttgattcat ccaatcgccg
     1261 tagaacaagg tactacattc tctatccgtg agggtggacg tactgttggt tcaggtatgg
     1321 ttacagaaat cgaagcttaa ttcgatttag ttcccagaag aacaattatt taagtcagac
     1381 actaaaagaa tcttgcttgg caaggttctt ttttggttct ttgtcaactg tagtgggttg
     1441 aaaaaaaact aagctcgaga aaggacaaat ttcgtccttt cttttttgat gttcagggcg
     1501 atgaaaatcc gttttttgaa gttttcaaag ttccgaaaac caaaggcatt gcgcttgata
     1561 agtttgatga gattattggt cgcttccaat ttggcgtttg aatagggtag ttgaagggcg
     1621 ttgacgattt tctttttgtc ctttagaaag gttttaaaga cagtctgaaa aagaggatga
     1681 acctgcttaa gattgtcctc aatgagtccg aaaaatttct ccggttcctt attctgaaag
     1741 tgaaacagca agagttgata gagctgatag tggtgtttca agtcttccga ataactcaaa
     1801 agcttgttta aaatctcttt attggttaag tgcatacgaa aagtaggacg ataaaatcgc
     1861 ttatcactca gtttacgact atcctgtttt atgagcttcc agtagcgctt gatagccttg
     1921 tattcatgag actttcgatg aaactgattc atgatttgga cacgcacacg actcatagta
     1981 cggctaagat gttgtacaat gtgaaagcga tccaacacga ttttagcatt cgggagtgaa
     2041 acagtctggg agactgtttc agcctgagcc tagaaatttg aaagcgaagc tgtttagcta
     2101 agtcatagta aggactaaac atatccatag taataatttt gacgcgacat cggacaactc
     2161 tatcgtagcg aagaaagtga tttcgaatga tagcttgtgt tctaccctca agaacagtga
     2221 tgatatcgag cttttcaaaa tcttgcgcaa tgaagctcat ctttcccttt gtaaaagcat
     2281 actcatccca agacataatc tcaggaagac gcgaaaaatc atgtttaaag tgaaa
//
LOCUS       DBERMR010000100         2239 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.100, whole genome shotgun sequence.
ACCESSION   DBERMR010000100 DBERMR010000000
VERSION     DBERMR010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.100"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..423
                     /locus_tag="ACL8A1_002134"
     CDS             <1..423
                     /locus_tag="ACL8A1_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202826.1"
                     /translation="KLIVILSISILVWTLVAFCTICVVYIFYGLLIPLLIYTFTLRII
                     LVSIGLILICFSLVIITRSIVVPMGMSLSFLLGLGQMLLQFSDAFLYFPIISTMNSFL
                     MTENQSYIPYTIGVAIQFLWGILLLSFSILLFWKRIVR"
     gene            454..1137
                     /locus_tag="ACL8A1_002135"
     CDS             454..1137
                     /locus_tag="ACL8A1_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011922424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGS1202827.1"
                     /translation="MKEKKVYQILAIDDDESILRLIKNSLERSEFEVTTRREVGNIDI
                     CSFIGFDLILLDIMMSIDGLEICKSIREQIDVPIIFVTAKQLDSDLASGVKAGADDYI
                     KKPFSITELVARVRMHIQREERNRKNNKEICTKNLIINVSNQEILIEEETISLTKREF
                     TILYTLASNPKRVYSVEELYERIYPSESDAQFRSIAEYIYQIRSKLKPYSINPIKTQW
                     GGGYSWNEN"
     gene            1122..2219
                     /locus_tag="ACL8A1_002136"
     CDS             1122..2219
                     /locus_tag="ACL8A1_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HGS1202828.1"
                     /translation="MERKLDFKTLVKKTKWTIVKQFCLNIIVAYIFPISTISIDIHSE
                     TQSIARDLLGVLFNVFSWVYICISLIIIVSINIKALLTRVKQEMEIVYHSSIWLTSVN
                     DSSNLTILEFIETNRHIESMQQRIKNMIQVEKDQKADILFKVSAMAHDLKTPLTVIKG
                     NSELLQLAPLSGIDVQCLKDIERASNQLNSYFNQLINYSKTFYSDQISFHQYRISNLS
                     KILEQECTYLIGDSIDYKYSTKIDRDCNIELDLDLIIRSVANIVNNAIAYADDAKKKI
                     RVTFELADSNITLSIWNNGSKFSDDVLENFGKLFYREDTSRNSQFEHFGIGLAFVKQV
                     MSIHSGDIQLQNKENGAMVKLIIPMKSSDYV"
BASE COUNT          773 a          276 c          401 g          789 t
ORIGIN      
        1 aaattaatag taatattatc aatatctata ttagtttgga ctctggttgc tttctgtact
       61 atttgcgtag tgtatatttt ttatggtcta ttaattccct tacttattta tacctttaca
      121 ttaagaataa tattggtatc aattggttta atattaatat gttttagttt agtgattata
      181 acaaggagta tagttgttcc aatggggatg tctttgtctt ttttgttggg gttgggtcaa
      241 atgcttctgc aattcagcga tgcttttctc tacttcccaa ttatctccac tatgaattca
      301 tttttgatga ctgagaatca atcttatata ccttatacaa taggtgttgc aattcaattt
      361 ttatggggga ttttgctgtt atctttctct attttgctat tttggaagag aatagtacga
      421 taaaaatttt gtattttgtg atattatata attatgaaag aaaaaaaagt atatcaaata
      481 ttagcaattg atgatgatga aagcattctt cgtttgataa agaattcttt agaacgctcg
      541 gagtttgaag ttacgacgag gagagaagta ggtaacatag atatttgttc attcattggc
      601 tttgatttaa ttttattaga tattatgatg tcgattgatg gtttagaaat ttgtaaatcc
      661 attcgagaac aaatcgatgt accgataatt tttgtaacag ccaagcaatt ggatagtgac
      721 ttggctagtg gagtaaaagc tggtgcggat gattatataa aaaaaccatt tagtattaca
      781 gaattagttg ctcgtgttag gatgcatatc caaagggagg aaagaaatcg taaaaataat
      841 aaagaaatat gcacaaagaa tttgataatt aatgtcagca atcaagaaat cttaattgaa
      901 gaagagacta tatcattgac aaagcgagaa tttacaattt tatatacttt agcaagcaac
      961 ccgaagagag tatattccgt agaggagctc tatgagagaa tatatccgtc ggaatcagat
     1021 gcacaatttc gttctattgc tgagtatatt tatcaaattc gttcaaaatt aaaaccatat
     1081 tcaattaatc cgatcaagac acagtgggga ggcggatata gttggaacga aaattagatt
     1141 ttaagacttt agtaaaaaag acgaagtgga cgattgttaa acaattttgt ttgaatataa
     1201 ttgtagctta tatttttcct atatcaacta tttctattga tattcattca gaaactcagt
     1261 caatcgctcg tgacttgtta ggggtattat ttaatgtttt ttcatgggtt tatatttgta
     1321 tatctttgat tattatagtt tctattaata ttaaagcctt gttgacaaga gtaaagcaag
     1381 aaatggagat tgtttatcat agcagtatat ggctaacctc tgtaaacgat tcatctaatt
     1441 taacaattct tgaattcata gagacaaata gacacattga atcaatgcaa caaagaataa
     1501 aaaatatgat tcaggtagag aaagaccaaa aggcggatat tctctttaaa gtttcagcga
     1561 tggcacatga cttaaaaaca cctttgactg tgattaaagg aaactcggaa ttgttgcaat
     1621 tggcaccttt atcaggtatt gatgttcaat gtctcaagga tattgaaaga gcaagtaacc
     1681 aattaaatag ctattttaat cagttaatca actactctaa aactttttac tctgatcaga
     1741 tttcttttca tcagtatcgt atcagtaatt tatccaaaat actggaacaa gagtgtacat
     1801 accttattgg agatagcatt gactacaaat atagtactaa gatagaccga gactgtaata
     1861 ttgaattgga tttagattta attatccgtt cggtggcaaa tattgttaat aatgctatag
     1921 cttatgcaga tgatgctaaa aagaaaatca gagttacctt tgagttagca gatagcaata
     1981 ttaccttaag tatttggaat aatggttcca aattttcgga tgatgtttta gaaaattttg
     2041 gaaaattatt ttatcgagaa gatactagca gaaattcaca atttgaacat tttggaatag
     2101 gtttggcatt tgtaaaacaa gtaatgagca ttcattcagg tgatatacaa ttgcaaaata
     2161 aggaaaatgg agctatggtt aaattaatta ttccgatgaa aagtagtgac tatgtctagg
     2221 cttgaatatc gtaattttg
//
LOCUS       DBERMR010000101         1967 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.101, whole genome shotgun sequence.
ACCESSION   DBERMR010000101 DBERMR010000000
VERSION     DBERMR010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.101"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            448..828
                     /locus_tag="ACL8A1_002137"
     CDS             448..828
                     /locus_tag="ACL8A1_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0115 family bacteriocin-like peptide"
                     /protein_id="HGS1202829.1"
                     /translation="MELVLPNNYVALEQEEMMYLDGGGVGRNWWNSRGSFATVLDVGL
                     AIYSGGATIYSAYAIKKAISANRGAITRTLRSLIIKHVGSAAGHLVNTALNVALTVTG
                     FSLGGAIAYGADWADGSLDGYIFT"
     gene            830..1036
                     /locus_tag="ACL8A1_002138"
     CDS             830..1036
                     /locus_tag="ACL8A1_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202830.1"
                     /translation="MKHFFAGIGEINFVSYLLYICVGIAPFFHVYIIGSEMNFVKIVL
                     SILGIIFVCILTIARIYRTFFYKE"
     gene            complement(1331..1657)
                     /locus_tag="ACL8A1_002139"
                     /pseudo
     CDS             complement(1331..1657)
                     /locus_tag="ACL8A1_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT          632 a          248 c          398 g          689 t
ORIGIN      
        1 aaaaaaatat tttgtattct agtatgtatt atatcattga ttagtctagc tataatcttt
       61 ccatggggct ggtcgatata atgtaaggga gcttgagttg ttagtagagg ataaaatgtt
      121 aagagtaata gtagttattc aatctttagt aataatactg cttgctgttg ctttattatt
      181 atctcggaaa aactttgatt cgttaaatgt tgttcttata atgatgatcg tatcacaatt
      241 gttgttatta gtatatttga taaaatcagg gaatcagtga ttagctgtta tttgaattaa
      301 gtaaactgtt aagcttcaag cagtaactgg ttttaaatat ttaaaataag ataaaggaag
      361 gaagtctaat ttaggtaact aaacaaagcc tgcgatcact tgttaggaaa ccatcctaag
      421 catgcttaat aaaaaaggag aaaataaatg gaactagtat taccaaataa ttatgttgct
      481 cttgagcaag aagagatgat gtatcttgat gggggtggtg ttggtcgtaa ctggtggaat
      541 agtagaggta gttttgcaac agttctggat gtaggtttgg ccatctatag tggtggtgca
      601 acaatttatt ctgcttatgc gataaaaaaa gctatctcag ctaatagagg ggctattacg
      661 agaacattac gtagtttaat aattaaacat gtaggtagtg cagctggcca tttagtcaat
      721 actgcactaa acgttgcact aactgttact ggattttcac taggtggagc aatcgcatat
      781 ggggctgatt gggctgacgg tagcttagat ggttatattt ttacttaaaa tgaaacattt
      841 ttttgcagga attggtgaaa taaactttgt aagttattta ttgtatatat gtgtaggaat
      901 agcccctttt tttcacgtat atataatagg ttctgaaatg aattttgtga aaatagtgtt
      961 atctattttg ggaattatat ttgtttgtat tttaactatt gctcgaattt ataggacttt
     1021 tttctataaa gaatagtttg gacttgaaat gtatttaaaa acaagaggtt ggtagattat
     1081 cagcctcttt cttgtcgttg aaaaagtgag atatattgac ttttgaaaaa aatgtcataa
     1141 ttgagttggg ataaacgtaa aagtcatact ttctcgttaa atcgttcggc gattcatggt
     1201 gacatggaca catagtagta aacacactat gtggcttggc agagctaaaa acttctggct
     1261 taacaatggc tggtattaag aactaacgaa caaataatat gtaaagagtc gaggaaatcc
     1321 ctcggctttt ttatctttcc ttcgcttggt aatagatctc tacttctttt ttgagatgag
     1381 acagcataga agtttcctcg gttagctcca gaagggagaa ccctttttgg ataagtttag
     1441 aatcgtctgt acaaatccca atacggacat agcagatagc cagcctatcg tagcttttct
     1501 tattcttggc gtcctctagt aaagtgtagc agatttgctt acacatgttt ttatcttgat
     1561 tgtatagata gagtgtggaa aggtttaata gaattgtcat tcgcaagtca tataaatgtt
     1621 gatagttttt atagacttct aagcgttgca agattttctc ggattattga caaataaacg
     1681 gacattctga tagacgattg ccctagcggc ttagtgtaaa ctttacaaat ttggcttgta
     1741 atttttttat aaaattcact tcagatactt gacagttatt atattttcgc ttatgcttat
     1801 tatatagata aaaggacggg ataggagttt aaaaatttga aagcgctatc cataatcata
     1861 acgcataatt ttctcctttt cgtgaaaaaa gttaacgtaa ttttgaaatg agcttaataa
     1921 gaggtgctat gtaggtagaa aataaagata ggagaaaaag tatgaat
//
LOCUS       DBERMR010000102         1813 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.102, whole genome shotgun sequence.
ACCESSION   DBERMR010000102 DBERMR010000000
VERSION     DBERMR010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.102"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            28..484
                     /locus_tag="ACL8A1_002140"
                     /pseudo
     CDS             28..484
                     /locus_tag="ACL8A1_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
     gene            649..948
                     /locus_tag="ACL8A1_002141"
     CDS             649..948
                     /locus_tag="ACL8A1_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HGS1202831.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKTSVDEANIKTLYSEKLIGISIPIK"
     gene            1278..1532
                     /gene="tnpB"
                     /locus_tag="ACL8A1_002142"
     CDS             1278..1532
                     /gene="tnpB"
                     /locus_tag="ACL8A1_002142"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGS1202832.1"
                     /translation="MDSLAYVVKTHFELDSFSGQVFLFCGGRKDRFKTLYWDGQGFWL
                     LYKRFENGKLTWLSTEKDVKALAPEQVDWLMKGFSITPQI"
BASE COUNT          602 a          338 c          326 g          547 t
ORIGIN      
        1 taataacgat actagataat gaggaaaatg caatggcaaa cccaactttc ggagaaaaaa
       61 agcgaataca gactatattg ctcgctatgg cgtatatgca gttatcccta atcctgaaca
      121 aaaacaaatt gttcttgttc aagaaccaaa tggtgcttgg ttcctaccat gtggagaaat
      181 tgaagcaggt gaaaatcatc aggaagccct aaagcgtgag ttgattgaag agcttggttt
      241 cacagcagaa attggtacct attacggaca agctgacgaa tatttctatt ctcgtcatcg
      301 tgatacctac tactacaatc ctgcctacct ctatgaagca acttctttca aagaagtaca
      361 aaagccacta gaaaacttta atcatattgc ctggttccct attgacgagg ctatccaaaa
      421 ccttaaacgt ggtagccata aatgggccat tgaatcttgg aaaaaacagc ataagattgg
      481 ctaatactct tcgaaaataa aattcaaacc acgtcagcgt cgccttaccg tactcaagta
      541 tagcctgcgg ctagcttcct agtttgctct ttgattttca ttgagtataa atcaagcgac
      601 tttattcaaa aagtgctata atagaattag aaaatcggag gaaatattat gaagcttatc
      661 aatacgacaa actcacactc gcaactggta aaaagtcagc tagaaagtac cgatgcaacc
      721 cttgttgagg tttattctgc agggaataca gatgttattt ttacccaagc tccgcttcac
      781 tatgaaatcc tcatctcaaa caaacaccgt gctattcgtg agcctgaaat cgaaaccatt
      841 caagaatttt tcttgaaacg taaaattgat aagacctctg ttgatgaagc caatatcaag
      901 acactctact cagagaaatt aattggaatt tcaatcccaa tcaagtaaga aaattgagta
      961 aaaactcgat ttgagagaaa gtccaataag tctttccata acaaaacgca tagtaacaag
     1021 agtttctaca cccactatta tgcgttttta tcattttgaa atctactgct aatcaatgaa
     1081 gaagcaaaga tcaaactagg cagctagtta caagttacgt aagcgcgtca taacaaggta
     1141 tctatcctaa ttccccaact tccagtatac tagataaaaa ataattatcg gaggaaaggt
     1201 attgtactat gataattcaa ctaagtgatt taggtcaagt tcaccttgtt tgtggtaaga
     1261 cagatatgag gtagggaata gactcattag cctatgtagt taaaacccac tttgaattgg
     1321 attctttctc cggtcaagtc tttctctttt gtggtggacg taaagaccgc tttaaaaccc
     1381 tttactggga tggtcaagga ttttggctac tatataaacg ctttgagaac ggaaaactga
     1441 cttggctaag tacagaaaag gatgtcaaag ctctcgcacc tgaacaagta gattggctga
     1501 tgaagggctt ttctatcact ccccaaatat agtagattga aactagaata gtacacctct
     1561 gcttctaaaa tattgttaga aatcgatttg actgtcctga tcgatttgtc ctgttcttgt
     1621 ttcgttttac tatagaacga tttgaaggcg tttatgatat ttagctgtac gagagtcttt
     1681 taaaagtgtt ttgatggttt ggatttcttc tttaattgat ttcatattac tattgtataa
     1741 tgctttttga ttttagtcta gtataaatag cgagaaatat ctatcctatc ttctagaatg
     1801 tcttccaaac gag
//
LOCUS       DBERMR010000103         1802 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.103, whole genome shotgun sequence.
ACCESSION   DBERMR010000103 DBERMR010000000
VERSION     DBERMR010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.103"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..396)
                     /locus_tag="ACL8A1_002143"
     CDS             complement(<1..396)
                     /locus_tag="ACL8A1_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HGS1202833.1"
                     /translation="MELVEFKNGHVNTVHEHIAITKVKEFLNNHDIPKDKGGIHKIST
                     DFFYNIIDMETTTEENRPWESHKEYYDVHYLLNGEEIILYNFLSQMELSEYKVDDDWQ
                     QMNGTALFSIKLKKDMLLLLEPNDAHKTGL"
     gene            complement(409..815)
                     /gene="cdd"
                     /locus_tag="ACL8A1_002144"
                     /pseudo
     CDS             complement(409..815)
                     /gene="cdd"
                     /locus_tag="ACL8A1_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221146.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
     gene            complement(902..1357)
                     /locus_tag="ACL8A1_002145"
     CDS             complement(902..1357)
                     /locus_tag="ACL8A1_002145"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HGS1202834.1"
                     /translation="MNDTNKNQYFVYEFDTSFVEMSYSQFFEGYCILFSKEPYPSLSD
                     MPMEFRKEFLMEMSILGDAMLEVLNAYRINYDILGNSTPVLHAHLFPRYMWEEEERRK
                     TVVHRYDSSVFSNPEFEFTIEKHGPIIKKLRSAIDRHYREYQQLLDDSN"
     gene            complement(1379..>1802)
                     /locus_tag="ACL8A1_002146"
     CDS             complement(1379..>1802)
                     /locus_tag="ACL8A1_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGS1202835.1"
                     /translation="FLSFLKKYVPGFENSFILDQGTRATNRSCRHIDNSSMMDEILDF
                     PMYTFKTMYSYQTPKEITYKSIVGGKLNNLFVIGKGAYQSESFRSQISCMLMGISSAA
                     AAKTIIEDKSNTLNINRDLFKSNLDILFTWEFSNWITD"
BASE COUNT          622 a          314 c          213 g          653 t
ORIGIN      
        1 taggcctgtt ttatgtgcat cgtttggttc caacaaaagt aacatatctt tctttaattt
       61 aatactaaat aaagctgtcc cattcatttg ttgccaatca tcatctactt tatattcaga
      121 caattccatt tgagacaaga aattatataa aattatttct tctccattta acagataatg
      181 gacatcataa tattccttat gactttccca cggtctattc tcttcagttg tagtttccat
      241 atcaattata ttgtaaaaga aatcagtaga aattttatgt atgccacctt tatcttttgg
      301 tatatcatga ttatttagaa actcttttac tttcgttatt gcgatatgct catgaaccgt
      361 attaacatgt ccatttttaa attcaactaa ttccatagtt ccctcctgtt ataaattttc
      421 ttgattaaaa ctaactggta gtagttcttt aagtttatat actttataag tatcaacatc
      481 actagccaat ataacttcaa atgaatcaga attacaaaat tctatcatta cctggcgaca
      541 cactccgcaa ggtgctataa aagatgataa gataccattt tttccaccta caatacatat
      601 tcttgaaaac gaacgttctc ctttaaatac agctttataa aaagatactc gttctgcaca
      661 gcaagtagga gaaaaagcag cattttctat attacatcca gaaaaaattt tttatcttca
      721 gttactaacg cagctccaac cttaaaatca gaataaggag cataagcttt ggccattgat
      781 ttaatagcct ctgatataat tgctttataa gtcattccta aaatatattt ttcaaaaaat
      841 tatatcatcc acaacctagt tgtaatatac aaggtattaa atctaatcga aaatgtggag
      901 ttcagtttga atcatctaat aattgttgat actctctata atgtctatca atagcagacc
      961 tcagtttttt aataattggt ccatgctttt ctatagtaaa ttcaaactca ggattgctaa
     1021 aaactgaaga atcatatcta tgaactacag tttttcttct ctcttcctct tcccacatgt
     1081 atctcggaaa taaatgagca tgaagtacag gtgttgaatt tcctaagata tcataattaa
     1141 ttctataggc atttaacact tcaagcatcg catcacctaa tatactcatt tccattaaaa
     1201 attcttttct aaattccatt ggcatatcag ataaagaagg atagggttct tttgaaaaaa
     1261 gaatacaata tccttcaaaa aactgagaat aagacatttc tacaaagcta gtatcaaatt
     1321 cataaacaaa gtattgattt ttattagtat cattcattta atattccctt attatttgtt
     1381 aatcagtaat ccaattagaa aactcccatg taaataatat atctaagttc gatttaaaca
     1441 aatctctatt aatgtttaga gtattgcttt tatcttcaat aatagttttt gccgctgctg
     1501 ctgatgatat ccccattaac atacaactaa tttgtgatct aaaactttcg gattggtaag
     1561 cacctttacc tattacaaac aaattattca attttccacc tacaattgat ttgtacgtta
     1621 tttctttagg agtttgatag ctatacatag tcttaaatgt atacattgga aaatcaagaa
     1681 tttcgtccat catagatgaa ttatcaatgt gcctacatga tctgttagta gctctagttc
     1741 cctgatcaag gataaaagaa ttttcaaaac ctggtacata ttttttaaga aaagataaaa
     1801 at
//
LOCUS       DBERMR010000104         1785 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.104, whole genome shotgun sequence.
ACCESSION   DBERMR010000104 DBERMR010000000
VERSION     DBERMR010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.104"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            4..77
                     /locus_tag="ACL8A1_002147"
     tRNA            4..77
                     /locus_tag="ACL8A1_002147"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38..40,aa:Asn,seq:gtt)
     gene            197..1035
                     /locus_tag="ACL8A1_002148"
                     /pseudo
     CDS             197..1035
                     /locus_tag="ACL8A1_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(1070..1785)
                     /locus_tag="ACL8A1_002149"
                     /pseudo
     CDS             complement(1070..1785)
                     /locus_tag="ACL8A1_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433508.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          570 a          302 c          358 g          555 t
ORIGIN      
        1 taatccgcca tagctcagtt ggtagtagcg catgactgtt aatcatgatg tcgtaggttc
       61 gagtcctact ggcggagtag tttagttaag aggtttagac ctcttttttg ttatgtaata
      121 ataatctatt tcgtgttata gtaaaatgaa ccaaaaatag tacacaatgt ggtataatct
      181 ttttatggca tagtcaatag attttcgtaa aaaagttctc tcttattgtg agcgaacagg
      241 tagtataaca gaagcatcac acgttttcca aatctcacgt aataccattt atggctggtt
      301 aaagctaaaa gagaaaacag gagagctaaa ccaccaagta aaaggaacaa aaccaagaaa
      361 ggttgataga gatagactta aaaactatct tactgacaat ccagacgctt atttgactga
      421 aatagcttct gactttggct gtcatccaac taccatccac tatgcgctca aagctatggg
      481 ctacactcga aaaaaagaac cacacctact atgaacaaga cccagaaaaa gtagccttat
      541 ttcttaagaa ttttaatagt ttaaagcacc tagcacctgt ttagattgat gaaacaggat
      601 tcgatactta tttttatcga gaatatggtc gctcattaaa aagtcagtta ataagaggca
      661 aagtatctgg aagaagatat cagaggattt ctttggttgc aggtctaaca aatgaatggt
      721 gagttaatcg ctccaatgac ttacgaagag acgatgacga gcgacttttt tgaagcttgg
      781 tttcagaagt ttctcttacc aacattaacc acaccatcgg ttattattat ggataatgca
      841 agattccata gaatggggaa gttagaactt ttatgcgaag agtttgggca taaactttta
      901 cctcttcctc cctactcacc tgagtacaat cctattgaga aaacatgggc tcatatcaaa
      961 aagcacctca aaaaggtatt accaagttgc aatacctttt acgaggctct tttgtcttgt
     1021 tcttgtttca attgactata ttagaggcga gacatttttc ggttctttgt caactgtagt
     1081 gggttgaaga aatcgaagat ctagaaagga caaatttcgt cctttctttt ttgaagtttt
     1141 caaagttcct aaaaccaaag gcattgtgct tgataagttt gatgagatta ttggtggctt
     1201 ccagtttggc gttggaataa ggtaattgaa ggccgttgac gattttttct ttatctttga
     1261 ggaaggtttt aaacagagtc tgaaaaagag gtggaaaagc aagagctgat agagattata
     1321 gctgatagag attatagtgg tgtttcaagt cttcgaatag ctcaaaagtt tatctagaat
     1381 ttctttatta gtcaagtgca tacgaaaagt agggcgataa aatcgtttat cactcagttt
     1441 ctgactatct tgttgaatga gctccagtag cgcttgatag ccttgtattc atgggatttc
     1501 gtatgatggc ttgtgttctg ctctcaagaa cagttatgat attgagctta tcaaagtcct
     1561 gagcaataaa gctcatctcc atctcccgat tgaaatagtc actccctgga ctgtttcaac
     1621 gtcctaggac ataatctcag gaagacgcga aaaatcatgc tcaaagtgaa aatcattgat
     1681 cttgcgaatg acagttgaag ttgaaataga caactgataa tcaatgtcgg tcatagaagt
     1741 cttttcaatt agcttctgag caatcttttg gttgatgata caagg
//
LOCUS       DBERMR010000105         1703 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.105, whole genome shotgun sequence.
ACCESSION   DBERMR010000105 DBERMR010000000
VERSION     DBERMR010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.105"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..84
                     /locus_tag="ACL8A1_002150"
     CDS             <1..84
                     /locus_tag="ACL8A1_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HGS1202836.1"
                     /translation="PILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            132..1607
                     /locus_tag="ACL8A1_002151"
     CDS             132..1607
                     /locus_tag="ACL8A1_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HGS1202837.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGMQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLLIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVAFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
BASE COUNT          475 a          346 c          344 g          538 t
ORIGIN      
        1 ccaatcttat tccgtatccc agcaatgagc gctccagtat ttgacaatat tgaagcagct
       61 attaaagaac acggattaag ctaattctaa tttgttaaca ttcatataac ataaaaaagg
      121 gaggaactgt tatggatgca atctttgacc taatcggaaa ggttttcaat cccatcttag
      181 aaatgggtgg acctgtcatc atgttaatca ttttgacagt attggcttta ctttttggag
      241 tgaaattctc caaagcgctt gaaggtggta tcaaacttgc catcgctctt acaggtatcg
      301 gtgctatcat cggtatgcta aatggtgctt tctcagcatc acttgcaaaa ttcgttgaaa
      361 acactggtat gcaattgagt attaccgacg ttggttgggc accacttgca acaatcactt
      421 ggggttctgc ttggacactt tacttcttgc tcatcatgtt gattgtcaac gtagtgatgc
      481 ttgctatgaa gaaaaccgat acacttgatg tcgatatctt tgatatctgg cacttgtcta
      541 tcacaggtct cttgattaaa tggtatgctg ataacaatgg tgtgagtcaa ggggtttcac
      601 tcttgattgc tacagcagct atcgttcttg tcggtgtgtt gaaaattatc aactctgact
      661 tgatgaaacc tacatttgat gaccttctta acgccccaag ttcatcacca atgacatcaa
      721 ctcacatgaa ctacatgatg aacccagtta tcatggtttt ggataagatt tttgaaaaat
      781 tcttcccagg ccttgataaa tatgactttg atgctgctaa attgaacaag aaaatcggtt
      841 tctggggatc taaattcttc atcggtttca tccttggtat cgttatcggt attatgggaa
      901 ctccacatcc aattgcaggt gttgcagatg cagataaatg gcgtcttgtt atcaaaggat
      961 ggttgtctct tggtttgact gccggtgtat ctttggaact cttctcactt atcggttcat
     1021 ggttcatcgc agccgtagaa ccactatcac aaggtattac aaatgttgct actaaacgtc
     1081 ttcaaggacg taaattcaat atcggtcttg actggccatt catcgctggt cgtgctgaaa
     1141 tctgggcttg tgccaacgta cttgcaccaa tcatgttgat tgaagcagtg cttctttcaa
     1201 aagttggaaa tggtatcttg ccacttgcag gtatcatcgc tatgggtgtt actccagctc
     1261 tcttggttgt aactcgtggt aaattgctcc gtatgattat cttcggaaca ctcttgttgc
     1321 cactcttcct tctttcaggt acccttattg caccatttgc aacagaactt gctaaaggtg
     1381 taggtgcctt cccagaaggt gtgagccaaa ctcaattgat tactcactct actcttgaag
     1441 gaccaatcga aaaacttctt ggttggacaa ttggtaacac tacaactggt gatatcaaag
     1501 caatccttgg tgcagtagcc ttccttgtat tctatatcgg tatctttgct tggtacagaa
     1561 aacaaatgat caaacgtaac gaagagtacg cagcaaaagc aaaataatgc gctcctataa
     1621 aatataaact caatgaaaat caaagagcaa actaggaagc tagccgcagg ttgctcaaaa
     1681 cacagttttg aggttgtaga taa
//
LOCUS       DBERMR010000106         1599 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.106, whole genome shotgun sequence.
ACCESSION   DBERMR010000106 DBERMR010000000
VERSION     DBERMR010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.106"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            280..462
                     /locus_tag="ACL8A1_002152"
     CDS             280..462
                     /locus_tag="ACL8A1_002152"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HGS1202838.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGEKIILGIDE
                     KNIDTYQRVNRLPAKL"
     gene            407..1256
                     /locus_tag="ACL8A1_002153"
                     /pseudo
     CDS             407..1256
                     /locus_tag="ACL8A1_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="programmed frameshift; internal stop; incomplete;
                     partial in the middle of a contig; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(1320..>1599)
                     /locus_tag="ACL8A1_002154"
     CDS             complement(1320..>1599)
                     /locus_tag="ACL8A1_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202839.1"
                     /translation="HAFFMDNVEWSHADSYFLSFVSDDVEERYTENVYLDSLSVKQKF
                     KFIFDFGDEWRFECQVLREIETEDEEAYLVRSVGTSPEQYPDYDGFDY"
BASE COUNT          576 a          302 c          272 g          449 t
ORIGIN      
        1 tataaaatta taactttgct agccgttgtt ataaaaagtt ttattcaatc attatctagt
       61 agaatagagt agtacatttt ataaattgtc atctctccat aaacacagta tatcattttt
      121 aaattttaag tcaaggaaaa ttaacccact ttaatttctt ttttaacccg tgttaaaaaa
      181 gaaattaact gagtaaaaac tctcgaaatt caattcgcta tattatcact caacttgaac
      241 aaataccaga accacaaaaa acagatgtaa aggaaaaata tggacttaaa atttgaaggg
      301 gttgatttag aatacaaaaa agctaaaaac aatcttccag agtctttctg ggaaacctac
      361 tcagcatttg caaatacaaa tggcgagaaa ataattctcg ggatagatga aaaaaacatt
      421 gacacctacc aaagagtcaa tagactccct gcgaaattat gaagcaagta aacaactaac
      481 cgatgcacga tttaaatgtc ttgttggtgt tcaacgcacg acttttgaag agatgttagc
      541 tgtattaaaa acagcttatc aacttaaaca cgcaaaaggt ggacgaaaac ctaaattaag
      601 cctagaagac cttcttatgg ccactcttca ctatgtgcga gaatatcgaa cttatgaaga
      661 aattgcggct gattttggta tccacgaaag caacttactc cgtcggagcc aatgggttga
      721 agtaactctt gttcaaagtg gtgttacgat ttcaagaact cctctcagct ctgaggacac
      781 ggtaatgatt attgatgcga cggaagtata aatcaatcgc cctaaaaaaa gaattagcga
      841 atgattctgg taaaaagaaa tgccacgcta tgaaggctca agcgattgtc acaagtcaag
      901 ggagaattgt ttctttggat atcactgtga actattgtca tgatatgaag ttgttcaaaa
      961 tgagtcgcag aaatatcgga caagctggta aaatcttgac tgacagtgat tatcaagggc
     1021 ccatgaagat atatcctcaa gcacaaactc cacgtaaagc cagcaaactc aaaccgctaa
     1081 cagctgaaga taaagcctgt aaccatgcac tatctaagga tagaagcaag gttgagaaca
     1141 tccttgccaa agtaaaaacg tttaaaatgt tttcaacaac ctatcgaaat catcgtaaac
     1201 gcttcggatt acgaatgaat ttgattgctg gtattatcaa tcatgaacta gaattttggt
     1261 ttcgcaggaa gtctattgaa atactaagcc tacacagact gatttcaatt ttaccattct
     1321 taatagtcaa aaccatcata atctggatat tgttctggcg acgttccaac cgaacgtacg
     1381 agataagctt cttcgtcctc tgtctcgatt tctctcagca cttggcattc aaaacgccat
     1441 tcatcaccga agtcgaaaat aaacttaaat ttttgtttga cacttaggct atccagatag
     1501 acattttctg tgtaacgttc ttcaacatcg tcactaacaa agctaagaaa gtaagaatct
     1561 gcatgactcc actcaacatt atccatgaaa aatgcatga
//
LOCUS       DBERMR010000107         1585 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.107, whole genome shotgun sequence.
ACCESSION   DBERMR010000107 DBERMR010000000
VERSION     DBERMR010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.107"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..1460)
                     /gene="psrP"
                     /locus_tag="ACL8A1_002155"
     CDS             complement(<1..1460)
                     /gene="psrP"
                     /locus_tag="ACL8A1_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078495769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-rich repeat glycoprotein adhesin PsrP"
                     /protein_id="HGS1202840.1"
                     /translation="MFFRRQKGEYRETDRVTRFKLIKSGKHWLRASTSLFGLFKVMRG
                     GVDTTQVMTETVEDKVSHSITGLDILKGIVAAGTVISGTVATQTKVFTNESAVLEKTV
                     EKTDALATNDTVVLGTISTSNSASSTSLSASESASTSASESASTSASTSASTSASESA
                     STSASTSISASSTVVGSQTAAATEATAKKVEEDRKKPASDYAASVTNVNLQSYAKRRK
                     RSVDSIEQLLASIKAAVFSGNTIVNGAPAINASLNIAKSETKIYTGTGRDSFYNIPIY
                     YKLTVTNDGSELTFTYTVTYVDPITETLGNLSRMTSGYSIYNSGTSTQTMLTLGSGLG
                     KPSGVKNSITDKNGRQVRHYNTSTMTKQGSGYTWANGAQMNGWQAKQGYGLTSSWTVS
                     ITGTDTSFTFTPYAAKTDKIRTNYFNGGGKVVESSTTSQSLSQSKSLSVSASQSASAS
                     ASTSASASASTSASESASTSASASASTSASESASTSA"
BASE COUNT          439 a          329 c          317 g          500 t
ORIGIN      
        1 gcactggttg acgctgattc agacgctgag gtgcttgctg aagccgacgc actcgttgat
       61 gccgattcag aagctgaggt acttgctgaa gctgaagcac ttgtacttgc tgaggctgag
      121 gcgctttgac tagcacttac tgagagtgac ttagactgtg aaagtgactg actggtcgtg
      181 ctagattcaa ctacctttcc tccaccattg aagtagttag ttctaatttt atctgtttta
      241 gcagcgtaag gggtaaatgt aaaggatgta tccgttccag taattgatac agtccaagat
      301 gatgttaatc catatccttg cttagcctgc caaccattca tttgggcacc atttgcccaa
      361 gtatacccac taccctgctt cgtcattgta gatgtattat agtgtcgaac ctgtctacca
      421 tttttgtcag taatggagtt ctttacacct gaaggtttac caagaccact gccaagggtt
      481 aacattgttt gtgttgaagt acctgaatta tagatagaat atccagaagt cattcttgaa
      541 agatttccaa gagtttctgt gataggatcc acatacgtaa ccgtataggt aaaggtcaat
      601 tctgaaccat catttgtcac tgtcaattta tagtaaattg gaatattata aaatgaatct
      661 ctacctgtac ccgtgtaaat ttttgtctca cttttagcaa tgtttagact tgcattaatt
      721 gcaggggcgc catttacaat cgtattgcca gaaaaaacag cagcttttat agaagccagc
      781 aattgctcga tggaatccac tgaacgcttg cgtcgcttag cataagattg gagattgaca
      841 tttgtaactg atgctgcata atcactagct ggtttcttac gatcttcttc gaccttctta
      901 gcagttgctt ctgtagcggc agctgtttgt gatcctacca cagtagatga tgcagagata
      961 cttgtcgaag ccgatgtact tgctgattct gatgcacttg tactcgctga ggtcgaagcg
     1021 ctggttgagg ctgactcaga tgcagatgta cttgccgact ctgaagctga caaactagtt
     1081 gaactcgctg aattacttgt agatatcgta cctagaacta ctgtatcatt tgttgccaaa
     1141 gcatccgttt tctctacagt tttttcaagt actgccgact catttgtaaa taccttcgtt
     1201 tgagttgcaa cggttccact tatgacagtt cccgcagcaa ctattccctt gaggatatca
     1261 agcccagtaa ttgaatgact tactttatct tctaccgttt cggtcatgac ctgagtagta
     1321 tcaacaccac cacgcatgac cttaaacaag ccaaaaagag aagtcgaggc ccgtagccaa
     1381 tgcttccctg atttgattaa tttgaaccga gttactcggt ctgtttctct atattcaccc
     1441 ttctgacgtc taaaaaatat tttgaaattt cctttcgagt attattttca tgtatggata
     1501 taaataaatt accatactat aattatagaa tagggaaccc aaaatagcaa gaaacttgag
     1561 gtatttttgc taaaaaaaaa aagca
//
LOCUS       DBERMR010000108         1460 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.108, whole genome shotgun sequence.
ACCESSION   DBERMR010000108 DBERMR010000000
VERSION     DBERMR010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.108"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..559
                     /locus_tag="ACL8A1_002156"
     CDS             <1..559
                     /locus_tag="ACL8A1_002156"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGS1202841.1"
                     /translation="EVPCEIPESWEWVRLNDITSYIQRGKSPKYSNIPIYPVIAQKCN
                     QWSGFSIDLARFIDPETVHSYQKERLLRDGDLMWNSTGLGTLGRLAIYHENKNPYGWA
                     VADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEEKASGSTKQKELLTKTIKEYLIP
                     LPPLPEQSRIVDKIEQFFAHIDALI"
     gene            616..1413
                     /gene="psrA"
                     /locus_tag="ACL8A1_002157"
     CDS             616..1413
                     /gene="psrA"
                     /locus_tag="ACL8A1_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HGS1202842.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
BASE COUNT          508 a          207 c          265 g          480 t
ORIGIN      
        1 ggaagtacct tgtgaaatac cagaaagttg ggaatgggtg aggttaaacg atataacctc
       61 ctacattcaa agagggaagt ctccaaaata ttcaaatatt cctatatacc ctgtgattgc
      121 tcagaaatgt aatcaatggt ctggcttttc tattgactta gcaagattta ttgatcccga
      181 aacagttcat agttaccaga aagaaagatt actaagagat ggtgatttaa tgtggaattc
      241 aacaggtcta ggtacgttag gacgattagc tatttaccat gaaaataaaa atccttatgg
      301 ctgggctgtt gcagatagcc atgttacagt tataagagtc ttatcgggag ttattaattg
      361 ccatttcatc tataattttc tttcctctcc tattgttcag tcagttattg aagaaaaagc
      421 gtcgggtagt acaaaacaaa aagaattatt aacgaaaact ataaaagaat atttaatccc
      481 cctcccaccc cttcctgaac aatccagaat cgttgataaa atcgaacaat ttttcgccca
      541 tattgacgca ctaatttagt ataatagttt tacgtgcggt ggaatttcta tcaaacataa
      601 gataggagtt ttacgttgaa agattttgaa ttacatttga agaaagctgg tttagctgaa
      661 aatactgtgc gttcgtacct ttatggtgtt cgatttttct tggagaatta cgaactcaaa
      721 atggaagatt tatttgaata caaaggatat ttattagaca attttaaacc taaaactgtc
      781 aatttgcgac tacaaggagt aaataaatat ctggctttta ttggtcatga tgatttgaaa
      841 ttaaaatttg tcaaagtaca gcaaaaaccg tttttagaag atgtcattag ccatgctgat
      901 tacctttttc ttaaacgtag tttgaaaaaa gatggtattt taaaatggca ttttgtcgtt
      961 tggtttttag gtgcaactgg ggctcgtgta agtgaactta ttaaactaaa agtagagcat
     1021 gtagaaatcg gttattttga catttactca aaaggtggta aaattcgtag actgtacatt
     1081 cctaaaaaat taagaaatag ttgtcttagt tggttagaat cagaaaatcg ccgaagtggt
     1141 tatttatttt tgaataaatt taacgaacca attacagcaa gaggagttgc tcaacagtta
     1201 aaaaattatg ctgataaata caaaatgaat cctaaagtaa tttaccctca ttcttttagg
     1261 catttatttg ctaagaattt tttagcgaag tataatgata ttgccttgct tgcagatttg
     1321 atgggacacg aaagtataga aactactcga atttatctaa ggaaaacagc tactgaacaa
     1381 caaaatattg tagataaaat tgttaattgg taaaaaataa caggtagtca aactgactac
     1441 ctgttatttt tgtgattatg
//
LOCUS       DBERMR010000109         1397 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.109, whole genome shotgun sequence.
ACCESSION   DBERMR010000109 DBERMR010000000
VERSION     DBERMR010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.109"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..>1397
                     /gene="pcpA"
                     /locus_tag="ACL8A1_002158"
     CDS             <1..>1397
                     /gene="pcpA"
                     /locus_tag="ACL8A1_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HGS1202843.1"
                     /translation="TAAVILAAYVPNEPILADTPSSEVIKETKVGSIIQQNNIKYKVL
                     TVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVASQAFSYYPDE
                     TGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRAFDFSELEEIE
                     LPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLTLPKSVKTLGS
                     NLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESYKTPKETKELA
                     SYSFNKNSYLKKLELNGGLEKIGTFAFADAIKLEEISLPNSLETIERLAFYGNLELKE
                     LILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEIHIKNKSTEFS
                     VKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETDVAKPKKNSNQ
                     GVVGWVKDKGLWYYL"
BASE COUNT          538 a          193 c          233 g          433 t
ORIGIN      
        1 acagctgcgg ttattttagc agcatatgtc cctaatgaac caatcctagc agatactcct
       61 agttcggaag taatcaaaga gactaaagtt ggaagtatta ttcaacaaaa taatatcaaa
      121 tataaggttc taactgtaga aggtaacata ggaactgttc aagtgggtaa tggagttact
      181 cctgtagagt ttgaagctgg tcaagatgga aaaccattca cgattcctac aaaaatcaca
      241 gtaggtgata aagtatttac cgttactgaa gtagctagtc aagcttttag ttattatcca
      301 gatgaaacag gtagaattgt ctactatcct agctctatta ctatcccatc aagcataaaa
      361 aaaatacaaa aaaaaggctt ccatggaagt aaagctaaaa ctattatttt tgacaaaggc
      421 agtcagctgg agaaaattga agatagagct tttgattttt ctgaattaga agagattgaa
      481 ttgcctgcat ctctagaata tattggaaca agtgcatttt cttttagtca aaaattgaaa
      541 aagctaacct tttcctcaag ttcaaaatta gaattaatat cacatgaggc ttttgctaat
      601 ttatcaaatt tagagaaact aacattacca aaatcggtta aaacattagg aagtaatcta
      661 tttagactca ctactagctt aaaacatgtt gatgttgaag aaggaaatga atcgtttgcc
      721 tcagttgatg gtgttttgtt ttcaaaagat aaaactcaat taatttatta tccaagtcaa
      781 aaaaatgacg aaagttataa aacgcctaag gagacaaaag aacttgcatc atattcgttt
      841 aataaaaatt cttacttgaa aaaactcgaa ttgaatggag gtttagaaaa aatcggtact
      901 tttgcatttg cggatgcgat taaacttgaa gaaattagct taccaaatag tttagaaact
      961 attgaacgtt tagcctttta cggtaattta gaattaaaag aacttatatt accagataat
     1021 gttaaaaatt ttggtaaaca cgttatgaac ggtttaccaa aattaaaaag tttaacaatt
     1081 ggtaataata tcaactcatt gccgtccttc ttcctaagtg gcgtcttaga ttcattaaag
     1141 gaaattcata ttaagaataa aagtacagag ttttctgtga aaaaagatac atttgcaatt
     1201 cctgaaactg ttaagttcta tgtaacatca gaacatataa aagatgttct taaatcaaat
     1261 ttatctacta gtaatgatat cattgttgaa aaagtagata atataaaaca agaaactgat
     1321 gtagctaaac ctaaaaagaa ttctaatcag ggagtagttg gttgggttaa agacaaaggt
     1381 ttatggtatt acttaaa
//
LOCUS       DBERMR010000110         1340 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.110, whole genome shotgun sequence.
ACCESSION   DBERMR010000110 DBERMR010000000
VERSION     DBERMR010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.110"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            1..111
                     /locus_tag="ACL8A1_002159"
                     /pseudo
     CDS             1..111
                     /locus_tag="ACL8A1_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558202.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(280..1340)
                     /locus_tag="ACL8A1_002160"
                     /pseudo
     CDS             complement(280..1340)
                     /locus_tag="ACL8A1_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_050084071.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          393 a          290 c          252 g          405 t
ORIGIN      
        1 atcaatcaaa aaattgctca aaagttaatt gaaaagattt ctatgactga tattgcccat
       61 cagctttcca tctcaacttc aactgttatt cgtaagctca atgacttcca cgtttacgag
      121 agtttcctcg tttggaagac attctagaag ataggataga tatttctcgc tatttataat
      181 ggattgaaat aagatatgaa caaattgatt aggaaaggaa aattgattta tagaaatgtt
      241 ttagcagtcg atgcgtactg ttatagattc aacgcactat aattgatgac gtttagctgt
      301 ttccaacaag ctcataataa tagctcttgc tttagctcct tcaaaacttt gagaaaataa
      361 ccaccctcgc ttttttaaac tgagctcagg ctaaaagagt ccactggact cttttactcc
      421 gtcccataac caatgattta atggcgcgtt cagctagatt attggaaagg accagatgtc
      481 cgtcttttaa aatagtctta aaggtttctt tatacttgag gctgtattca attgcccttc
      541 ctagttttga acctgctaaa actgactgac gacggcacca agcaaagaag tcttccatta
      601 ggggttggag ctcttcttga cgtttctgta gtcgttcatc agctgacaat gtcctcccag
      661 tctctttcca aggcaaataa ctgatcacaa taagctaaac ctttagctcc taaggatgat
      721 ttatctgctt gcttgggggt cgcttcaaaa aacttccttc tcacatgtac ccaacatcca
      781 acaagttcag cttcttccag ttgacaataa gcttctagcg gttcgcagtt taccaagctt
      841 agcgtcgcct tgctcctaaa ccttggactg ctatcgcata ggcagaacta gaggactaaa
      901 gtcctaagtt actgccgcaa catatcacaa tgcacatagc cagaataatc tcctaggaat
      961 tcttgtacta ccgaaccact tcgccactga tcatagtggt aaagcgtaat cccttgtttc
     1021 tctgctttac ctgacaaaaa agtccaatag taagtcagct gactatcact ttctagaacc
     1081 cgataggagg tttcatccgc atgaagtaag ggctgagtca atagtctctc tcgcaagagg
     1141 ttataaaggg gctccaaata gtattgactc gccttgatat gccaattaga gatttcctta
     1201 cgtgtgattg gtaaacccat cctagcccaa tcttcttctt ggcgataatt gggtaccttc
     1261 agattaaact tctgatggat ggtgtgagcg ataatagaag ctgagtcaaa gctatgcgcc
     1321 aaaggggctt taggaatagg
//
LOCUS       DBERMR010000111         1331 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.111, whole genome shotgun sequence.
ACCESSION   DBERMR010000111 DBERMR010000000
VERSION     DBERMR010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.111"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..932)
                     /gene="pspC"
                     /locus_tag="ACL8A1_002161"
     CDS             complement(<1..932)
                     /gene="pspC"
                     /locus_tag="ACL8A1_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     LPXTG-anchored form"
                     /protein_id="HGS1202844.1"
                     /translation="MFKSNHERRMRYSIRKFSVGVASVAVASLFMGSVVHATEKEGST
                     QAATSFNRGNGSQAEQRGELDLERDKAMKAVSEYVGKMVRDAYVKSDRKRHKNTVALV
                     NQLGNIKNRYLNEIVHSTSKSQLQELMMKSQSEVDEAVSKFEKDSFSSSSSGSSTKPE
                     TPQPENPEHQKPTTPSPDTKPSPQPEGKKPSVPDINQEKEKAKLAVVTYMSKILDDIQ
                     KHHLQKEKHRQIVALIKELDELKKQALSEIDNVNTKVEIENTVHKIFADMDAVVTKFK
                     KGLTQDTPKEPGNKKPSAPKPGMQPSPQPEVKPQP"
     gene            1135..>1331
                     /locus_tag="ACL8A1_002162"
     CDS             1135..>1331
                     /locus_tag="ACL8A1_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HGS1202845.1"
                     /translation="MEQLHFITKLLDIKDPNIQILDIINKDTHKEIIAKLDYEAPSCP
                     ECGSQMKKYYFQKPSKIPYLET"
BASE COUNT          357 a          259 c          229 g          486 t
ORIGIN      
        1 ggctgcggtt taacctctgg ttgaggactt ggttgcatac ctggttttgg agcagatggt
       61 tttttgttac ctggttcttt tggtgtgtcc tgagttaagc cttttttgaa tttagtcaca
      121 actgcatcca tgtctgcaaa tatcttgtgg actgtatttt caatttctac tttggtattt
      181 acattatcaa tttcagaaag agcttgcttt ttaagctcat caagctcctt aataagagca
      241 acaatctgac gatgtttttc tttctgcaga tgatgttttt gtatatcatc taaaatcttg
      301 ctcatgtagg ttactacagc aagcttagct ttttcttttt cctgattaat gtctggtacg
      361 cttggtttct tgccttctgg ttgagggctt ggtttggtat ccggagatgg agttgttggt
      421 ttttgatgct ctggattttc cggctgcgga gtttctggtt tagtggagga tcctgaactt
      481 gacgaagaaa atgagtcctt ttcaaattta gacacagctt catctacttc tgattgactc
      541 ttcatcatca gttcctgtag ttggcttttt gaggttgaat gaactatttc attcaaatac
      601 ctgttcttaa tgtttcccaa ctggttaact agagctacag tatttttatg tcgttttcta
      661 tctgatttta cataggcatc tctcaccatt tttcctacat attcactgac cgctttcatt
      721 gccttatctc gttctaaatc gagttctcca cgttgttctg cctgacttcc atttccccta
      781 ttaaaagaag tggctgcttg ggtacttccc tctttctctg tcgcatgtac aacacttccc
      841 ataaaaagac tggcaacagc tacgctagct actcctacac taaatttacg aatggaataa
      901 cgcattcttc gctcatgatt tgatttaaac atgtctatat tctccataat tttaattaac
      961 tacagtatat cctgctaata tgaattgtat atgaaatttg ctacattatt tggctctata
     1021 atatttgtag tgggtaaatc ccctatggat attatggagc ctattttgtg tagaaaaaaa
     1081 gtcccatatg acctataatg aaaagcgaca aaacaactca ttagaaagaa tcatatggaa
     1141 caattacatt ttatcacaaa attactagac attaaagacc ctaatatcca gattttagac
     1201 atcatcaata aggatacaca caaggaaatc atcgccaaac tggactatga agctccatct
     1261 tgtcctgagt gcggaagtca aatgaagaaa tattactttc aaaaaccttc taaaattcct
     1321 tatcttgaaa c
//
LOCUS       DBERMR010000112         1330 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.112, whole genome shotgun sequence.
ACCESSION   DBERMR010000112 DBERMR010000000
VERSION     DBERMR010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.112"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..1242
                     /locus_tag="ACL8A1_002163"
     CDS             join(<1..332,332..1242)
                     /locus_tag="ACL8A1_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGS1202846.1"
                     /translation="MVKLIDRYGIEIVKKGKNRHYSSKLKQEMMDKALLEGCSQRSIS
                     LDYALPNQGMLSFWPAQYKKNGYTIVEKTRGRPAKMGRKRKKTWEEMTELERLQEENE
                     RLRTEVAYPKKVKRVRGKGRSPRARKAETVREMVSGGFRLDFLLETARLARSTYYYQL
                     KQLDGVDKDKEIKTEIQGIDNEHKGNYGYRRIHLELRNRGFVVNHKKVQRLMRILGLT
                     ARIRRKRKYSSYQGEIGKKAENLIQRQFEASRPMEKCYTDVTEFAIPNSTQKLYLSPV
                     LDGFNSEIIAYHLSTSPNLEQVKSMLEQAFTEKYYENTILHSDQGWQYQHDSYHRFLE
                     SKEIQASMSRKGNSPDNGMMESFFGILKSEMFYGYEKNFRSLENLEQAIVDYIDYYNN
                     KRIKVKLKGLSSVQYRTKSFG"
BASE COUNT          469 a          223 c          284 g          354 t
ORIGIN      
        1 catggtgaaa ttaattgacc gttatggcat agaaatcgtt aaaaaaggga agaatcgtca
       61 ctattcttct aaattgaaac aagaaatgat ggataaagcc ttactggaag gttgttcaca
      121 aagaagtatt agtcttgatt atgccctccc aaaccaagga atgctttcat tttggccggc
      181 acaatacaag aaaaacgggt atactattgt tgagaaaaca agagggagac cagctaaaat
      241 gggacgtaaa cggaagaaga cttgggaaga aatgacagaa ctagaacgac tccaggagga
      301 gaatgaacgc ttacggactg aggtggccta cctaaaaaag ttaaaagagt tagaggaaag
      361 ggacgaagcc ctagagcgag aaaggcagag acagttagag aaatggtttc aggaggattt
      421 cgactagatt ttcttcttga aacagcacgt ttagctcgct cgacttacta ttatcagttg
      481 aaacaactgg atggggttga taaagataaa gagattaaaa ccgaaattca gggcattgat
      541 aatgaacata aaggcaacta tggctatcgt cgaatccatt tagaactaag gaatcgtggt
      601 tttgtggtca atcataagaa agttcaacgg ttgatgagaa ttcttggttt aacggctcga
      661 attcgccgta aacggaagta ttcttcctac caaggagaga ttggcaagaa agcagagaat
      721 ctcattcaac gccagttcga agcatctagg ccaatggaaa agtgctatac agatgtgaca
      781 gagtttgcca ttccaaatag cacacagaaa ctctatttat caccagtttt agatggtttt
      841 aacagtgaaa tcattgctta tcatctttct acttcgccaa acttagaaca agtgaagagt
      901 atgttagaac aggcctttac agagaaatac tatgagaata cgattctcca tagtgaccaa
      961 ggctggcaat accaacacga ttcttatcat cggttcctag agagtaagga aattcaagca
     1021 tccatgtcac gcaagggcaa cagcccagac aacggcatga tggaatcttt ctttggcatt
     1081 ttaaaatccg aaatgtttta tggctatgag aagaacttta gatctttaga aaaccttgaa
     1141 caagctattg tggactacat tgattactac aacaacaaac gaattaaggt aaaactaaaa
     1201 ggacttagtt ctgtgcagta cagaactaaa tcctttggat aaattatttg tctaattttt
     1261 tggggtcagt acagtattaa ataccatgat cttatgcgtt ttataataaa aaatctgttc
     1321 ttgttttatt
//
LOCUS       DBERMR010000113         1178 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.113, whole genome shotgun sequence.
ACCESSION   DBERMR010000113 DBERMR010000000
VERSION     DBERMR010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.113"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            239..>1178
                     /locus_tag="ACL8A1_002164"
     CDS             239..>1178
                     /locus_tag="ACL8A1_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSpn8 family transposase"
                     /protein_id="HGS1202847.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTI
                     HNEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQ
                     KFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGK
                     SIEERPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQFIRLIPNKTAESVN
                     QALTLLLGEHRILSITADNGSEFKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRR
                     WLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSEF"
BASE COUNT          412 a          209 c          244 g          313 t
ORIGIN      
        1 agccacctta ccttagtcaa gaattagatg tttcactatg tttgagtaag ttgatgattt
       61 catttgataa caggtttgaa actgttaggc taggtggcca aggctaatca tagccttggt
      121 ttagctgaaa aaacaggttc aagggttcct gttgtcaaat gaaatgtgat ttaaggtata
      181 agaaaacacc tctgtgctat acttgttgtt caccacaaac acaaggaaag gcacagagat
      241 gcaagaacat tataccccaa aagggaaaca tttgacaata gataaccgtc gcttgattga
      301 gcggtggaag aatgaaaata agtccaatcg tgaaattgca ggcttgttag gaaaggcgcc
      361 tcaaacgatt cataatgaag tcaaaagagg tacaacttta caacaagtga gaaaagggct
      421 atacaaaaag gtctattctg ccgattacgc acaaactgtt taccaattca atcgaaaacg
      481 gtcggtgaaa aagttaattt taacaaagga aatcagagag aagatcttac actatcataa
      541 gcaaaaattt tcgcctgaaa tgatggttaa caagaagcaa gtgaaagttg gtatttcaac
      601 catctactac tggtttcata atggtcattt aggattgacg aaggccgaca tgctttatcc
      661 cagaaaaagg aaaggtgtca agaagcaagc tagtccgaac tttaagccgg caggtaaatc
      721 tatcgaagaa cgtcctgacg ttattaatct tcgcttggaa aatggtcatt atgaaattga
      781 taccgtccta ctgactaaga taaaaaatta ttgcctgtta gtcttaaccg accggcggag
      841 cagacaccaa tttataaggt taattccaaa taaaactgct gaatctgtca atcaggcgct
      901 tacgttacta ttaggggagc atcgtattct gtccattact gcagataatg gttcggagtt
      961 caaacgattg tctgaggtat ttcctgagga acatatctac tacgcacatg cttactcttc
     1021 atgggagaga ggttcaaatg aaaatcataa tcgattaatt cggagatggt tacctaaagg
     1081 aaccaagaaa acgactccga aagaagtagc ttttatcgaa aattggatta acaactaccc
     1141 taaaaaatgc ttggactaca agtcgccaag tgaatttc
//
LOCUS       DBERMR010000114         1143 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.114, whole genome shotgun sequence.
ACCESSION   DBERMR010000114 DBERMR010000000
VERSION     DBERMR010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.114"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..375)
                     /locus_tag="ACL8A1_002165"
     CDS             complement(<1..375)
                     /locus_tag="ACL8A1_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202848.1"
                     /translation="MRKSGFLIYYVLFCIVCFLLGLLLSFILKNETFATIPPKLELQF
                     TLTVLQANSTNFLLYLFCFPISPLLQLFDLLSLGIQLGISGQMIGIVATIKGILPHAL
                     IEIPNFIWYQGMSQYLLYRLLKD"
     gene            complement(372..>1143)
                     /locus_tag="ACL8A1_002166"
     CDS             complement(372..>1143)
                     /locus_tag="ACL8A1_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202849.1"
                     /translation="CLIAIQIISLILPSLFSIISKLFLFLSIGLLSIEQGQVVIYVIL
                     LNYFILVMGILDNHASNFYFKSSRMSQKRFRSSLFILLRFCWLNKGLTFSFILISILL
                     SYLFWYMMTMLSIDDTKIPILFLYTSIYITLMEAIIGNHKSSIGVDLALLQLSALNHS
                     KMNFFFKNAQLMMLTHLISFLQFSLLLCLIPVTSYSNDIKSILLNLLLIPFNYFIAYV
                     YYKKALFLIKGTVSLLRWSFLVLYCFLIIFVILVGGIR"
BASE COUNT          491 a          173 c          138 g          341 t
ORIGIN      
        1 atccttcaat aatcggtata aaagatactg actcatacct tgataccaaa taaaattcgg
       61 tatttcaata agagcatgag gtaatattcc ttttattgta gctactattc caatcatctg
      121 accagatata cccaattgta ttcctaaaga tagcaaatca aaaagttgca ataagggcga
      181 aatcggaaaa cagaatagat atagcaaaaa atttgtagaa tttgcctgta aaactgtcaa
      241 tgtaaattgt aattccagtt tcggaggtat agtagcaaaa gtctcatttt ttaatataaa
      301 gcttaaaagt aaacctagta aaaaacagac tatacaaaat aacacatagt aaatcagaaa
      361 accacttttt ctcatctaat gccccctact aaaattacaa atattatcag aaaacaatat
      421 aaaaccagaa aactccatct caataaacta actgtacctt taattaaaaa tagagctttc
      481 ttataataga cataggctat aaaataattg aatggaatca ataacaaatt caacaaaata
      541 ctttttatat cattactata acttgtcacc ggaatcaaac ataacaacaa tgaaaattgt
      601 aagaaagaga ttaaatgcgt caacatcatt agttgtgcat tcttaaaaaa gaaattcatt
      661 ttagaatgat taagagcaga taattgaagt aaagctagat ctaccccaat tgaggattta
      721 tgattcccta ttattgcctc cattaatgta atatatattg aggtataaag aaataatatt
      781 ggtatttttg tgtcatcaat tgataacatc gtcatcatat accaaaatag ataggaaaga
      841 agaatactaa tcaaaataaa agaaaaagtt aaccctttat ttaaccaaca aaaacgaagc
      901 aatataaata gactgctacg aaatcttttt tgactcattc gactgctttt aaaatagaaa
      961 ttactcgcat gattatctaa tatccccatg actaaaataa aataattgag tagtatgaca
     1021 taaattacaa cttgtccttg ttctatagac aatagtccta tagataaaaa taagaacaat
     1081 ttacttataa ttgaaaacaa actaggtaga attaaagaaa taatttgaat agctatcaaa
     1141 caa
//
LOCUS       DBERMR010000115         1110 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.115, whole genome shotgun sequence.
ACCESSION   DBERMR010000115 DBERMR010000000
VERSION     DBERMR010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.115"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..837
                     /locus_tag="ACL8A1_002167"
     CDS             <1..837
                     /locus_tag="ACL8A1_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HGS1202850.1"
                     /translation="FHFKHDFSCLPEIMSWDEYAFTKGKMSFIAQDFNNLNIITVLEG
                     RTQAIIRNHFLKYDRAVRCRVKIITMDMFSPYYDLARQLFPCAKIVLDRFHIVQHLSR
                     AMSRVRVQIMNQLDRKSHEYKAIKRYWKLIQQDSRKLSDKHFYRPTFRMHLTNKEILN
                     KLLSYSQDLKHHYQLYQLLLFHFQNKEPEKFFGLIEDNLKQVHPLFQTVFKTFLKDKE
                     KIVNALQLHYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERTKFVLS
                     RA"
BASE COUNT          352 a          221 c          187 g          350 t
ORIGIN      
        1 tttcacttta aacatgattt ttcttgtctt cctgagatta tgtcttggga tgagtatgct
       61 tttacaaagg gaaagatgag cttcattgcg caagatttta acaatctcaa tatcatcact
      121 gttcttgagg gaagaacaca agctatcatt cgaaatcact ttcttaaata tgatagagcc
      181 gtccgatgtc gcgtcaaaat tattactatg gatatgttta gtccttacta tgacttagct
      241 agacaacttt tcccatgtgc taaaatcgtg ttggatcgct ttcacattgt acaacatctt
      301 agccgtgcta tgagtcgtgt gcgtgttcaa atcatgaatc aattggatcg aaagtctcat
      361 gaatacaagg ctatcaagcg ctactggaag ctcatacaac aggatagtcg taaactcagc
      421 gataaacatt tttatcgccc tacttttcgt atgcatttaa ccaataaaga gattttaaac
      481 aagcttttga gctattcaca agacttgaaa catcactatc agctctatca actcttgctg
      541 tttcactttc agaataagga accggagaaa tttttcggac tcattgagga caatctaaag
      601 caggttcatc ctctttttca gactgtcttt aaaacctttc taaaggacaa agagaaaatc
      661 gtcaacgccc ttcaactaca ctattctaac gccaaattgg aagcgaccaa taatctcatc
      721 aaacttatca agcgcaatgc ctttggtttt cggaactttg aaaacttcaa aaaacggatt
      781 tttatcgctc tgaatatcaa aaaagaaagg acaaaatttg tcctttctcg agcttagctg
      841 acttcaaccc actacagttg acaaagagcc aaaaaaaact ccgtagtctc tagcgctata
      901 aatccggcta gagattacag agttattttt taatctaaac ttcctacaag ggtatattat
      961 ctactgaaag gatatggtat ttgtagggaa tactttaaag agttcttaca ggaagtcagt
     1021 attaactagt tatattaagt ttagtttacc cattcaccat tggcattgac tctatagcta
     1081 tctacagttg tgttgactgc aagggcacct
//
LOCUS       DBERMR010000116         1073 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.116, whole genome shotgun sequence.
ACCESSION   DBERMR010000116 DBERMR010000000
VERSION     DBERMR010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.116"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..460)
                     /locus_tag="ACL8A1_002168"
     CDS             complement(<1..460)
                     /locus_tag="ACL8A1_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202851.1"
                     /translation="MKFQTNNSLYKLMNLIIYLFLLYLFIDLLMKSGILDEQIDKLSE
                     LSNLFTIKIETILTVISVVFTFIATLIEYGFSLFLITLFFDKNKRNFGDILLSKNITL
                     SFSILLCYLFNPSDIKFAYFSVLTVLLIGITNYTRFKNLRLTVVFSVPFLF"
     gene            complement(441..1037)
                     /locus_tag="ACL8A1_002169"
     CDS             complement(441..1037)
                     /locus_tag="ACL8A1_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS1202852.1"
                     /translation="MKIENESKSYDKCILKNISLEIDKRIYRVEGENGSGKSTLLQLL
                     AGLDTFDSGLKNCVSKDVLYLDTNQLGVVPFTIEENLQLLCDSFQIHLSFEDKEQINT
                     FFDNKIGDNYMTASTGTKFKLGLSLIFAKNWDYIFIDETLSTVDARSIDMIAKRLISM
                     KESSTIIYVSHNLLNQELISKSQRILIEEGRVYEISNK"
BASE COUNT          408 a          168 c          119 g          378 t
ORIGIN      
        1 taaataaaaa tggtacagaa aacactactg tcaatcttaa atttttaaat ctcgtataat
       61 ttgttatccc aattagtaat actgtcaata cgctgaaata ggcaaacttt atatccgatg
      121 gattaaataa ataacaaagt aaaatgctaa aacttaaggt tatattttta gataaaagaa
      181 tatctccaaa gttcctctta ttcttatcaa aaaataatgt aatcagaaag agagaaaatc
      241 catattcaat aagagttgct ataaatgtga aaacaacaga aatcactgtc aatattgttt
      301 ctattttaat tgtaaaaaga ttagatagtt cagataattt atcaatttgt tcatctaaaa
      361 ttcccgactt catcagtaaa tcaataaaca aatataaaag aaaaagataa ataatcagat
      421 tcattaattt gtataaacta ttatttgttt gaaatttcat aaacccgtcc ttcctctatt
      481 aaaatccttt gagatttgct aatcaattct tgattcaata aattatgaga tacatatata
      541 atagtagagc tttctttcat ggaaattaag cgtttagcaa tcatatcaat acttcttgca
      601 tccactgttg ataaagtctc atcaataaaa atataatccc aatttttagc aaaaattaag
      661 gataagccta atttgaattt tgttccagta gatgctgtca tatagttatc tccgatttta
      721 ttatcaaaaa aagtattaat ttgttcttta tcctcaaatg atagatggat ttgaaaagaa
      781 tcacacaaca actgcaaatt ttcttctata gtaaatggaa caaccccaag ctgattggta
      841 tcaaggtata gtacatcttt ggagacacaa ttctttaaac cactatcaaa agtatctagc
      901 ccagctaaaa gttgtagaag ggtgctcttt cctgaaccat tctctccttc aactctgtaa
      961 attctcttat caatctctag tgatatattt ttcaatatac atttatcata acttttagat
     1021 tcattttcaa ttttcataat ttaattccct ctatcaacgc tgctaaaatt aat
//
LOCUS       DBERMR010000117         1063 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.117, whole genome shotgun sequence.
ACCESSION   DBERMR010000117 DBERMR010000000
VERSION     DBERMR010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.117"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            128..>1063
                     /locus_tag="ACL8A1_002170"
     CDS             128..>1063
                     /locus_tag="ACL8A1_002170"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HGS1202853.1"
                     /translation="MKLSHVPVTLNNKKIQEFMRNGFILDSNTLVTEINKLEYFSYIS
                     VNNTLRICGIDYNDSNNYTKEQVLKNWDSMLRESILRVYSEAGEANITLSSGFDSNYI
                     LYILANYTNSSINAFCIGGEKGINEIPEVTKIAKFYGINLLVDTIMSQDLEYFPDIVW
                     RLEGSLFECGVILQYYLGRLLFQKGNTSILCGESADEIMTFKYHSVNYNQFCNDKQKS
                     VYFSYSDYPFYVTNSIVLKKNSLLLHSFGIHPRYPYKMSEIVEMSKKISNLNDKKEFH
                     KKNCELRFQDSVLDNINSVPGTTHLFSCLNIQTLVK"
BASE COUNT          396 a          123 c          168 g          376 t
ORIGIN      
        1 aattataatt tttgatacga agacaaaaga attaaaaatt tttcaagata taacgacttc
       61 atactttaat ttatactata ctgtttatgg aggtgtgttc tattatagta catctttaaa
      121 gaaagtgatg aagttatctc atgttcccgt taccttaaat aacaaaaaaa tacaagaatt
      181 tatgagaaat ggatttattt tagattcaaa tactttagta accgaaatta ataaattaga
      241 atatttctct tacatttctg taaataatac tcttcgtata tgtggtattg attataatga
      301 ttcaaacaat tatactaaag aacaagtatt aaagaattgg gactctatgc tacgtgaatc
      361 tatattaaga gtgtatagtg aagcaggaga ggctaatatt acactatcat ctggttttga
      421 ttcaaattat atattatata tattagcgaa ttatacgaat tcttcaatta atgcattttg
      481 cataggagga gaaaaaggaa ttaatgagat tcctgaagta actaaaattg caaaatttta
      541 tggtataaat ttattagttg atactataat gagccaagat ttagaatatt ttcccgatat
      601 agtatggaga ttggaaggaa gtctctttga atgtggtgtc atactgcagt actatttagg
      661 caggttgttg ttccaaaaag ggaacacaag tattctttgt ggcgagtcag cggatgaaat
      721 aatgactttt aagtatcatt ctgtgaacta caatcaattt tgtaatgata aacagaagtc
      781 agtatatttt agttattccg actatccatt ttatgttaca aattcaattg ttttaaagaa
      841 aaatagccta cttttgcata gttttggcat tcatcctaga tatccatata aaatgtctga
      901 gattgttgaa atgtctaaaa aaatatcaaa tctaaacgat aaaaaagaat ttcacaaaaa
      961 gaactgtgag ttgaggtttc aagatagtgt tcttgataac ataaatagtg tcccgggtac
     1021 aactcattta tttagttgtt taaatattca aacattagta aaa
//
LOCUS       DBERMR010000118         1053 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.118, whole genome shotgun sequence.
ACCESSION   DBERMR010000118 DBERMR010000000
VERSION     DBERMR010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.118"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..559
                     /locus_tag="ACL8A1_002171"
     CDS             <1..559
                     /locus_tag="ACL8A1_002171"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGS1202854.1"
                     /translation="NKDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGT
                     EIPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTLLMSFKLSIGKVAILDIP
                     ATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPI
                     SNHEEMKRIISKVDLLFQKVSQLFE"
     gene            complement(507..1052)
                     /locus_tag="ACL8A1_002172"
     CDS             complement(507..1052)
                     /locus_tag="ACL8A1_002172"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGS1202855.1"
                     /translation="MNWVVIKIKDIFSINTGLSYKKGDLSINNKGVRIIRGGNIKPLE
                     FSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHIGKFARIDKDYDGVVAGGFIF
                     QLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQALYNIPKTTLSELLIPLAPFE
                     EQELITQKVEKLFEKVNQLWK"
BASE COUNT          380 a          169 c          152 g          352 t
ORIGIN      
        1 gaacaaagac gaaacaacct cttatcctat atatgaaatc ccagaagcat ggagatatat
       61 taaatttgct agcctagtta acttcagaat aggaaaaact cctccacgca gtgaagctac
      121 tttttgggga actgaaatac cctgggtatc tatatcagat atgcctatat ctggttatgt
      181 aactaatacg agagaatcta tttctaaatt agcattaaaa tcaaagaaaa tagacatttc
      241 acctaaagga actttattaa tgagctttaa attatctatt ggaaaagttg ccatattgga
      301 tattcctgcc actcataatg aagccattat atcgatattc ccctatgcta ataaagaaaa
      361 tattatcaga gattacttaa tgatattttt gccacttatc tctactttag gtgattcaaa
      421 agatgctatc aaagggaaaa cattaaatag taccagtatc tccgaattat taatccctat
      481 ttctaatcat gaagagatga aaagaatcat ttccaaagtt gatttacttt ttcaaaaagt
      541 ttctcaactt tttgagtaat aagttcctgt tcctcaaaag gagctaacgg aattaatagc
      601 tcgcttagtg tagttttagg aatattatat aaagcttgac ctgatagttt agttattgct
      661 ttcaattgtt tataaaataa cggagaggac aagttaaata atagaaattt tgaaataatc
      721 tctgaacttt cgaatggtgt taattggaaa ataaatccac cagccacaac accatcatag
      781 tctttatcga ttcttgcaaa ctttccaata tgttctaaag aggttgatac aggtgttatt
      841 agctgattat gttttaaata aacttgctca gaggagatga attgtgtatc aatgtagtaa
      901 tcattatcca acagagaaaa ttctaaaggc ttaatattac caccacgtat aattctaaca
      961 cctttattat taatgcttaa atcgcccttc ttgtaagaaa gacctgtatt tattgaaaaa
     1021 atatctttta tttttataac aacccaattc ata
//
LOCUS       DBERMR010000119          980 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.119, whole genome shotgun sequence.
ACCESSION   DBERMR010000119 DBERMR010000000
VERSION     DBERMR010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.119"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            67..815
                     /locus_tag="ACL8A1_002173"
                     /pseudo
     CDS             67..815
                     /locus_tag="ACL8A1_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
     gene            828..>980
                     /locus_tag="ACL8A1_002174"
     CDS             828..>980
                     /locus_tag="ACL8A1_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HGS1202856.1"
                     /translation="MNIAVIGLGHVGLAYALLFASKYKVVAYDIDSVKINNLKKGILP
                     SKNEELM"
BASE COUNT          340 a          113 c          191 g          336 t
ORIGIN      
        1 ttttgatgat gtggaattta atcagacact tagtgagttt ttagataaat tgtagaggga
       61 atggttatga gtaacaatac taagtatatc tttttatctc cacatttaga tgacgcaatt
      121 ttttcctgtg gtgattatat ttcaaaatta acttcagaag gagaaattgt acttgttatt
      181 actatatttt ctggttaccc tttgagccaa cagctacagc cctctgctaa gcaatttcat
      241 aagttgtgta accttgggaa atatccgatt gaagaaagga agaaagaaga caggttagct
      301 tgtgaacgtc tacaatgtga ttttagacac ttatcgtact atgaatgtct ctataggaaa
      361 gatagaaatg gcaattttct ataccgtcat atctatagtg aattaaaaaa tgaggacaca
      421 ttaaagaatg atatcataaa agagctgtta atgcatttag acgacaaatg tgtcgtatat
      481 tgtccgcttt cacttggtga ccatgttgct catgtttttg tgaatagtat tggaagggcg
      541 cttgaattta tgcgttataa agtaatatat tatgaggact tcccatatgt aagtgatagt
      601 agtatggtta gttatatggg aaaaactaaa gaattaaaaa tgtatcaaga ggaacttgat
      661 gaaaaaatta tattgataga atatcatcaa tattgtgcta taaatctcag attttgataa
      721 tctggaaatc agttgaaaaa cttttaaaca atattaaaga gttgtattta aggaatgggg
      781 cagcttattc tattagattt tggattaaga aataaggaag tagttttatg aatatagcag
      841 taattggttt ggggcatgtt gggctggcct acgcgttact atttgcatct aaatataaag
      901 ttgttgcata tgatatagat tctgtaaaaa taaataattt aaaaaagggc attcttccat
      961 ctaaaaatga agagcttatg
//
LOCUS       DBERMR010000120          925 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.120, whole genome shotgun sequence.
ACCESSION   DBERMR010000120 DBERMR010000000
VERSION     DBERMR010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.120"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..784
                     /locus_tag="ACL8A1_002175"
     CDS             <1..784
                     /locus_tag="ACL8A1_002175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001840385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGS1202857.1"
                     /translation="YMLKKLDKNGFGKVKTVITCHGWVEYNLKKKFLTYFDFWTYSMG
                     DAFICVSETMKKRLESIIKNKKIVAINNGINVSNSDLDVVGVQDFKKEFCISNNKKII
                     CYVGRLDPEKRPDRFLEFAEKLFLVREDVIFIMAGNGSMWAALKEKICHLKYRDNFRL
                     LGEIYPATIVYQISDLLYIPSDTEGIPMCVLESMSQGTPVLASNVGGLSEIIEHRVDG
                     FLFEKEDVEGVCACANFLLNDSEYLKYIGENSKSKIRKHESI"
     gene            808..>925
                     /locus_tag="ACL8A1_002176"
     CDS             808..>925
                     /locus_tag="ACL8A1_002176"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGS1202858.1"
                     /translation="MDRNIDQELVSIIIPTHNRYESLIRAVKSCLHQSYKNIE"
BASE COUNT          345 a           80 c          195 g          305 t
ORIGIN      
        1 atatatgtta aaaaaacttg acaagaatgg atttggaaag gttaagacag tgataacatg
       61 ccatggatgg gttgaatata acttaaaaaa gaaattcttg acttattttg atttttggac
      121 atattctatg ggagatgctt ttatttgtgt atctgaaact atgaaaaaaa gattggagag
      181 tataataaaa aataagaaga tagttgcaat aaataacgga attaatgtat caaatagcga
      241 tttagatgta gtaggtgttc aagactttaa aaaagaattt tgtatttcaa ataacaaaaa
      301 aatcatttgt tatgttggaa ggttggatcc agaaaaaagg ccggatagat tccttgaatt
      361 tgcagaaaaa ttatttttag tcagagaaga tgtgattttt attatggctg gaaatggtag
      421 tatgtgggct gctttgaaag aaaaaatttg ccatttgaaa tatagagata atttcaggtt
      481 acttggtgaa atctatccag ctactatagt ttaccaaata tcggatttat tgtatattcc
      541 ttctgataca gaaggaatac ctatgtgtgt tttagagtca atgtcacaag gaactccggt
      601 tttagctagt aatgttggcg ggttaagtga aattattgaa catagggttg atggattttt
      661 atttgagaag gaagatgttg agggagtgtg tgcttgtgct aattttttac tcaatgattc
      721 tgagtatttg aaatatatag gtgagaatag taaatcaaaa ataagaaaac atgagagtat
      781 atgatgaatt attagagaag agtagtcatg gataggaata tagatcaaga attagtaagt
      841 attataatac caacacataa taggtatgaa tctctgatta gagctgtgaa aagttgttta
      901 catcaaagtt ataaaaatat cgaag
//
LOCUS       DBERMR010000121          916 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.121, whole genome shotgun sequence.
ACCESSION   DBERMR010000121 DBERMR010000000
VERSION     DBERMR010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.121"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            203..679
                     /locus_tag="ACL8A1_002177"
     CDS             203..679
                     /locus_tag="ACL8A1_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGS1202859.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEQLFDQVATLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAEATA"
     gene            717..>916
                     /locus_tag="ACL8A1_002178"
     CDS             717..>916
                     /locus_tag="ACL8A1_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HGS1202860.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPG"
BASE COUNT          322 a          145 c          170 g          279 t
ORIGIN      
        1 cctagtttgc tctttgattt tcattgagta ttagaacaga aaaatgcttg gagtatttgt
       61 ttgtgtgttt atttttatat aacaaactat aaacaaaaca aaaatataaa aaaagagaca
      121 aaaaagaaca gaaagtaatt gacaacggtt tcatatagtg ttatacttat ggcataaagt
      181 taatggaaag aagggaaaac ttatggtatt agattatttc tttgacaaaa accttgtgtt
      241 ttgcttagaa gcggataatc aagaacaact ctttgatcag gttgcaactt tattggaaga
      301 acgagaaatt gtcactccaa cttatcgtga agccttgatc acgcgtgaaa agtcatttcc
      361 aactggttta gatatggaat ttctaggaaa ggacttgcca aatgtagcga ttcctcatac
      421 agatattgtt cataatctag ctgagaaagt ggtggttgtt cgattagaaa aaccagtaac
      481 ttttcacaat atgatagctc ctgataagga agtagaagta tccttgctct tctttatcat
      541 caataactca agttcaagtc aaacaaatat tctggctcag ttgatggact ttttcacagg
      601 aaatggacat cttgaagacc tatcaaaaat ttccgaacca gaaaaacttt atgcttacat
      661 tgctgaagca accgcttaat cttgtctatt aaaaaataaa atcggaggaa atctaaatga
      721 ttaaaattct tgctgcctgc ggtgcaggtg ttaactcaag tcaccaaatt aaaagtgctc
      781 tagaagaaga actttcaaac cgtggttatg atgttcactg tgatgcagtc atggtgaaag
      841 atgtaaacga agaccttatg aaaggttacg atatctttac accaatcgct gcaacagacc
      901 ttggttttga accagg
//
LOCUS       DBERMR010000122          909 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.122, whole genome shotgun sequence.
ACCESSION   DBERMR010000122 DBERMR010000000
VERSION     DBERMR010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.122"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            317..909
                     /locus_tag="ACL8A1_002179"
                     /pseudo
     CDS             317..909
                     /locus_tag="ACL8A1_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          315 a          161 c          168 g          265 t
ORIGIN      
        1 ctttttttag atattgaact aatactcaat gaaaatcaaa gagcaaacta taatatattg
       61 aaactagaat agtacacatc tacttctaaa acattgttag aaatcgattt gattgtcttg
      121 attgatttgt cttgttctta tttcattttg ttatagaaag ttagctgcag actgctcaaa
      181 acactgtttt taggttgtag atagaactga cgaagtcagt aacatctata cgacaaggcg
      241 aagctgacgc ggtttgaaga gattttcgaa gagtataata ctagactcaa atcaaaaagc
      301 attatacaat agtaatatga aatcaactaa agaagaaatc caaaccatca aaacactttt
      361 aaaagacttt cgtacagcta aatatcataa acgccttcaa atcgttctat ttcgtctgat
      421 gggcaaatct tataaagaga ttatagatct tttagactgt aatcaaacaa cgatttggcg
      481 aaatgtaaaa aaatatgagg agttcggact cgactctctc cttcaagaaa cacgtggtgg
      541 tcgtaaccat gcatatatga cagttgagga agagaaagcc tttcttgccc gccatttgaa
      601 ggctacagag gcaggagaat ttgttacaat accttatttc aggcttatat cgttcctaca
      661 cacgtgatgc cttctatcaa ctgttgaagc accatggttg gcgaaatatt atgccacgtc
      721 cagaacatcc taggaaagca gacgctcaaa ccattgtcgc gtctaaaaat aaaatctcaa
      781 ttcaagaaga caagaaagcg ctttaaaacc agtagacctt ttcataaggt tcgcttgatg
      841 taccaagatg aggctggttt cggtagaatc agtaaactgg gatcttgttg gtctccaata
      901 ggagtaggt
//
LOCUS       DBERMR010000123          865 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.123, whole genome shotgun sequence.
ACCESSION   DBERMR010000123 DBERMR010000000
VERSION     DBERMR010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.123"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            50..855
                     /locus_tag="ACL8A1_002180"
                     /pseudo
     CDS             50..855
                     /locus_tag="ACL8A1_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          298 a          151 c          180 g          236 t
ORIGIN      
        1 agatttttca atagacttcc tgcgaaacaa aatatggtat aatagttcta tgaattatga
       61 agcaagtaaa caactaaccg atgcacgatt taagcgtctt gttggtgttc aacgcacgac
      121 ttttgaagag atgttagctg tattaaaaac agcttatcaa cttaaacacg caaaaggtgg
      181 gcgaaaacct aaattaagcc tagaagacct tcttatggcc actcttcaat atgtgcgaga
      241 atatcgaact tatgaagaaa ttgcggctga tcttggtatt cacgaaagca acttaatccg
      301 tcggagccaa tgggttgaag taactcttgt tcaaaggtgg tgttgcgatt tcaagaactc
      361 ctctcagttc tgaggacacg gtaatgattg atgcgacgga agtaaaaatc aatcgaccta
      421 aaaaaagaat tagcgaatga ttctggtaaa aagaaatttc acgctatgaa gactcaagcg
      481 attgtcacaa gtcaagggag aattatttct ttggatatcg ctgtgaacta ttgtcatgat
      541 atgaagttgt tcaaaatgag tcgcagaaat atcggacaag ctggtaaaat cttggctgac
      601 agtggttatc aagggctcat gaagatatat cttcaagcac aaactccacg taaatccagc
      661 aaactcaagc cgctaacagc tgaagataaa gcctgtaacc atgtgctatc taaggagaga
      721 agcaaggttg agaacatctt tgccaaagta aaaacgctta aaatgttttc aacaacctat
      781 cgaaatcatc gtaaacgctt cggattacgg atgaatttga ttgttggtat tatcaatcat
      841 gaactaggat tctagttttg cagga
//
LOCUS       DBERMR010000124          856 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.124, whole genome shotgun sequence.
ACCESSION   DBERMR010000124 DBERMR010000000
VERSION     DBERMR010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.124"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..>856
                     /gene="phtD"
                     /locus_tag="ACL8A1_002181"
     CDS             <1..>856
                     /gene="phtD"
                     /locus_tag="ACL8A1_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtD"
                     /protein_id="HGS1202861.1"
                     /translation="ITSRTARGVAVPHGNHYHFIPYEQMSELEKRIARIIPLRYRSNH
                     WVPDSRPEQPSPQPTPEPSPSPQPAPNPQPAPSNPIDEKLVKEAVRKVGDGYVFEENG
                     VSRYIPAKDLSAETAAGIDSKLAKQESLSHKLGAKKTDLPSSDREFYNKAYDLLARIH
                     QDLLDNKGRQVDFEALDNLLERLKDVSSDKVKLVDDILAFLAPIRHPERLGKPNAQIT
                     YTDDEIQVAKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTHSHWIKKDSLSEAERA
                     AAQAYAKEK"
BASE COUNT          277 a          185 c          181 g          213 t
ORIGIN      
        1 atcacaagtc gaaccgccag aggtgtagct gtccctcatg gtaaccatta ccactttatc
       61 ccttatgaac aaatgtctga attggaaaaa cgaattgctc gtattattcc ccttcgttat
      121 cgttcaaacc attgggtacc agattcaaga ccagaacaac caagtccaca accgactccg
      181 gaacctagtc caagtccgca acctgcacca aatcctcaac cagctccaag caatccaatt
      241 gatgagaaat tggtcaaaga agctgttcga aaagtaggcg atggttatgt ctttgaggag
      301 aatggagttt ctcgttatat cccagccaag gatctttcag cagaaacagc agcaggcatt
      361 gatagcaaac tggccaagca ggaaagttta tctcataagc taggagctaa gaaaactgac
      421 ctcccatcta gtgatcgaga attttacaat aaggcttatg acttactagc aagaattcac
      481 caagatttac ttgataataa aggtcgacaa gttgattttg aggctttgga taacctgttg
      541 gaacgactca aggatgtctc aagtgataaa gtcaagttag tggatgatat tcttgccttc
      601 ttagctccga ttcgtcatcc agaacgttta ggaaaaccaa atgcgcaaat tacctacact
      661 gatgatgaga ttcaagtagc caagttggca ggcaagtaca caacagaaga cggttatatc
      721 tttgatcctc gtgatataac cagtgatgag ggggatgcct atgtaactcc acatatgacc
      781 catagccact ggattaaaaa agatagtttg tctgaagctg agagagcggc agcccaggct
      841 tatgctaaag agaaag
//
LOCUS       DBERMR010000125          852 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.125, whole genome shotgun sequence.
ACCESSION   DBERMR010000125 DBERMR010000000
VERSION     DBERMR010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.125"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(61..669)
                     /locus_tag="ACL8A1_002182"
     CDS             complement(61..669)
                     /locus_tag="ACL8A1_002182"
                     /inference="COORDINATES: protein motif:HMM:NF016726.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HGS1202862.1"
                     /translation="MQIKFIDKVSNLIMLNLLYVASVVTVIAIGSGESALIATLIKIV
                     RHEESYPYRDFANSFFKDYWKNLGAALISNLPILILLFSLFFLPYIPLPIYIISILRH
                     IGVIYIILHLIATTFLIPLIGRYNNTLKNSLHNSIMLAYKHFFIAVLIRIIEIIPVLL
                     FFILQNQLLVWITLMIFILPSITKYANAFLYNFIFSKYEKLN"
BASE COUNT          336 a           91 c          130 g          295 t
ORIGIN      
        1 tatcgttgaa attagttaaa aatttttata aaatcaagat gcaaggctat gagatgttct
       61 ctagtttaat ttttcatact tcgagaatat aaagttatat aaaaatgcat tagcatattt
      121 tgttattgag ggaagtataa aaatcattaa agttatccaa actagtaatt gattctgtaa
      181 tataaagaag agtagaactg ggataatttc aataattcta ataagaacgg caataaaaaa
      241 atgtttatag gctaacatga ttgaattgtg tagagaattt tttaaagtgt tattatatct
      301 accgattaaa ggaattagaa atgttgttgc tattaaatga agtatgatat atattactcc
      361 aatatgtcgt aagatagaaa tgatatatat aggtaatggt atgtagggta ggaagaacaa
      421 actgaataat aagattaaaa ttggtaaatt agatattaat gctgctccta aatttttcca
      481 ataatcttta aaaaaactgt ttgcgaagtc tctataggga taggattcct cgtgtcgtac
      541 aatttttatt agagttgcta ttagggcact ttcacctgat ccgatagcaa taactgttac
      601 tactgacgct acatagagta gatttaacat tataagattt gaaactttat caataaattt
      661 tatctgcatc tttaactcct gctgttttta tacttataat atcacatcat ttgagtttca
      721 tcaactttga atacattaag aaagaatata actatgagga aaggaaaaaa ttattatgaa
      781 taaatccaaa aaatataaag aaagaattca ttatgaatac gataatctta ctaaatcaga
      841 gaaaaaattg ct
//
LOCUS       DBERMR010000126          714 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.126, whole genome shotgun sequence.
ACCESSION   DBERMR010000126 DBERMR010000000
VERSION     DBERMR010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.126"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..>714)
                     /locus_tag="ACL8A1_002183"
     CDS             complement(<1..>714)
                     /locus_tag="ACL8A1_002183"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202863.1"
                     /translation="ALVDADSDAEILAEADALVDAEAEALVLAEADALVLAEADALVD
                     ADSEALVDAETEALVDAEAEALVLAEADALVDAEAEALVLAETDALVLADSDALVLAE
                     AEALVDAEADALVDAEADALVDAEAEALVLAEADALVDAEAEALVDAEADALVLAEAE
                     ALVDADSDADVLAEAEALVDAEAEALVLAEADALVDAEADALVLADSEALVLAEAEAL
                     VDAEADALVDAEADALVDAE"
BASE COUNT          181 a          228 c          166 g          139 t
ORIGIN      
        1 ttccgcatca acgagtgcgt cagcctcagc atcaacaagt gcgtcggctt cggcatcaac
       61 aagtgcctca gcctcagcaa gtacaagtgc ctcagagtca gcaagtacca gtgcgtcagc
      121 ttcagcatca acgagtgcgt cagcctcagc aagcaccagt gcttcagctt cagcgtcaac
      181 aagtgcttca gcctcagcaa gcacatcagc gtctgaatct gcatcaacga gtgcttcagc
      241 ttcagcaagc accagtgcgt cggcttcagc gtcaacgagt gcttcggctt cagcatcaac
      301 gagtgcgtca gcctcagcaa gtaccagtgc ctcggcttca gcatcaacga gtgcgtcagc
      361 ctcagcatca acaagtgcgt cggcttcggc atcaacaagt gcctcagcct cagcaagtac
      421 aagtgcatca gagtcagcaa gtaccagcgc atcagtctca gcaagcacca gtgcttcagc
      481 ttcagcatca acgagtgcgt cagcctcagc aagtaccagt gcctcggctt cagcatcaac
      541 gagtgcttca gtctcagcgt caaccagtgc ctctgaatcc gcatcaacaa gtgcatcggc
      601 ttcagcaagc accagtgcgt cggcttcagc aagtactagc gcctcagcct cagcatcaac
      661 cagtgcatca gcctcagcaa gtatctcagc gtctgaatcg gcatcaacaa gtgc
//
LOCUS       DBERMR010000127          688 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.127, whole genome shotgun sequence.
ACCESSION   DBERMR010000127 DBERMR010000000
VERSION     DBERMR010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.127"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..>688
                     /locus_tag="ACL8A1_002184"
     CDS             <1..>688
                     /locus_tag="ACL8A1_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HGS1202864.1"
                     /translation="NIRQSVKSLETDTQNKLNQKLSQAEFEVRAGSIRQEILNVTKDK
                     ASKSELTQTAEELSSKIASVQASGRNLFLNSLFKQDIPKTGIWTTSTYTATIDSESKY
                     LGHKALKIIGLNPSGRDGGNPKVTYPALGQFGKVIPGSTTNQDVTISFYAKANKNGIM
                     LRSRLGNIGYKTGNVTLSTEIKRYVVHIPKGWTNESKQTTNEWLFNFNQEGTIWIWMP
                     KFEISDVDTSY"
BASE COUNT          237 a          121 c          156 g          174 t
ORIGIN      
        1 aatattaggc aatctgtgaa gagtcttgag acagacacgc agaacaaact aaatcagaag
       61 ttgagtcagg ctgaatttga ggtgcgagct ggctctattc gtcaggaaat cctgaacgta
      121 accaaggata aagccagcaa gtcagaactt acgcagacag ctgaggagct ctctagtaag
      181 atagcgagtg tgcaggcatc cggtcgaaat ctattcttga actcactatt caagcaggat
      241 attccaaaaa caggaatttg gacaacgagt acatatacgg ctactatcga tagcgaaagt
      301 aagtatcttg gacacaaggc tcttaaaatt ataggtttga atccatctgg ccgtgatgga
      361 ggtaatccca aggttactta tccagctctg ggtcaattcg ggaaagtaat tcccggaagt
      421 acgactaatc aagatgtaac cattagtttt tatgctaagg caaataaaaa tggaataatg
      481 ctaagatctc gattagggaa tatcggatat aaaactggaa atgtgacatt gtcgacagaa
      541 attaagcgat atgttgtcca tattccaaaa ggttggacaa acgaatccaa gcagaccaca
      601 aatgaatggt tgttcaattt caaccaggaa ggaaccattt ggatttggat gccgaagttt
      661 gaaataagcg atgtagatac ttcttatt
//
LOCUS       DBERMR010000128          678 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.128, whole genome shotgun sequence.
ACCESSION   DBERMR010000128 DBERMR010000000
VERSION     DBERMR010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.128"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            93..455
                     /locus_tag="ACL8A1_002185"
     CDS             93..455
                     /locus_tag="ACL8A1_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HGS1202865.1"
                     /translation="MLEVGLNFLISLFTFTFDILYPIVKVGHTDDYSHGAVKLSVSLV
                     DKDAMKKISVTVIGYFEINIDENITDILYVNGTAILYHYLRSIVSIVSAIDSSEAMLL
                     PTINVLELLDKSQPFEEE"
BASE COUNT          224 a           87 c          123 g          244 t
ORIGIN      
        1 cgtcagttcc atctacaacc tcaaaacagt gttttgagca acctgaggct agtttcctag
       61 tttgctcttt ggttttcatt gagtataaca gattgttaga agttggttta aatttcctaa
      121 tcagtttgtt cacatttacc ttcgatatat tatatcccat agttaaggtt ggtcatacag
      181 atgattatag tcatggagcc gtaaaactta gtgtttcttt agttgacaaa gatgccatga
      241 aaaaaatatc tgtaactgta ataggatatt ttgaaataaa tatagatgaa aatatcaccg
      301 atattctata cgtaaatggt actgctattc tttatcatta tttacgttct attgtttcaa
      361 tagtttcggc aattgatagc agtgaagcaa tgttgctacc taccattaat gttttagagt
      421 tactagataa atctcaacct tttgaagaag aataatttat tagctcacta aattgagggt
      481 aaggaaaagt aaaatcagta agaaaaatgt cttgcattat acagcaacct tttgggaatg
      541 agtagatgga ttgaataaaa ttttattaag agtggatgat ttatctgtag attattattg
      601 gacagttagt cttgaagtag tctaagaatt aggttataat cagtagaatc cttgctaata
      661 atgaggaggt tagtttat
//
LOCUS       DBERMR010000129          662 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.129, whole genome shotgun sequence.
ACCESSION   DBERMR010000129 DBERMR010000000
VERSION     DBERMR010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.129"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..>662)
                     /locus_tag="ACL8A1_002186"
     CDS             complement(<1..>662)
                     /locus_tag="ACL8A1_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HGS1202866.1"
                     /translation="EALSRTKNELAGVKSAQATYKETTTRRLSELTNLANGKANKSEL
                     TQTAEELASKIASVQVGGRNYIRGTKRMMLARGLWASGTFRPSGAGTAKTIDVSDSPA
                     TGFDKAIRLTSSNARDQIGIAQDGFYISQGTYTMSCWVKGRRGQKVKLQTYWQVNDNS
                     GISPIFTLKDENWTKLSFTSARNRAGVASIGYVYLVNAEVGEYLDVLAPQLEDGSLAT
                     SS"
BASE COUNT          150 a          181 c          128 g          203 t
ORIGIN      
        1 ttgagcttgt tgccaaactt ccgtcttcca gctggggcgc aagaacatct aaatattctc
       61 cgacctcagc atttacgaga tacacatagc caattgatgc gactccagcc ctatttctag
      121 cgctagtaaa cgatagcttt gtccaatttt catcctttaa tgtaaagatg ggcgaaatac
      181 ccgaattatc attgacttgc caataagttt gtagcttgac cttttgacct cttctgcctt
      241 tgacccaaca agacatcgtg tatgtgcctt gcgagatata aaatccatct tgagcaatac
      301 ctatttggtc tctagcattg cttgaggtca atcgtatcgc tttatcaaag ccagttgctg
      361 gactgtctga tacatcaatc gtctttgccg tcccagcgcc tgacggtcta aaagtacctg
      421 atgcccacaa tcctctggct agcatcatgc gctttgtacc ccggatataa ttcctacccc
      481 cgacctgcac actcgctatc ttactagcta gctcctcagc tgtctgcgtg agttctgact
      541 tgttggcttt accattggcc aagttggtca gttctgacag tctacgagtc gtcgtctcct
      601 tatacgtcgc ttgcgctgac ttcacgccag ccagttcatt cttagtccgg ctaagtgctt
      661 ca
//
LOCUS       DBERMR010000130          650 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.130, whole genome shotgun sequence.
ACCESSION   DBERMR010000130 DBERMR010000000
VERSION     DBERMR010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.130"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..>650
                     /locus_tag="ACL8A1_002187"
     CDS             <1..>650
                     /locus_tag="ACL8A1_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGS1202867.1"
                     /translation="IIHQFGYTNHRIKLILSNEDLGATNARNIGIKNSRGKYISFLDD
                     DDEYMPDRILKLMACFKKSRMKNLALVYSYGIIIYPNGTREEEKTDFVGNPLFVQMVH
                     NIAGTSFWLCKKEVLELINGFEKIDSHQDGVVLLKLLVQGYQIDIVREFLVNYYAHSK
                     ENGITGVTQKTINADEEYYNYCRKYFNLLSFNERILVTKKYYSLNIKRLLLIGDKC"
BASE COUNT          246 a           70 c          124 g          210 t
ORIGIN      
        1 aaattatcca tcaatttggc tataccaatc atagaattaa gttaatttta agtaatgaag
       61 atttaggtgc aactaatgcc agaaacatag gtatcaaaaa ttctagaggt aagtatatat
      121 catttttaga cgatgatgat gaatatatgc cagatcgaat tttaaagttg atggcttgtt
      181 ttaaaaagag tagaatgaag aatttagctt tagtttatag ttatggaata ataatttatc
      241 caaatggtac acgagaagag gagaagaccg attttgttgg aaatcccttg tttgttcaaa
      301 tggttcacaa tatagcaggt acgtcatttt ggttgtgtaa aaaagaggtg ctagaattaa
      361 ttaatggttt tgagaaaata gattcacatc aggacggtgt tgttttatta aaactacttg
      421 ttcaaggata ccaaattgat atagtgcgag aattcttggt gaattactac gctcacagta
      481 aagaaaacgg tatcactgga gtgacacaaa aaacaattaa tgcagatgaa gaatattata
      541 attactgtag gaaatatttt aatttattga gtttcaacga gagaatattg gttacaaaga
      601 aatattattc tttaaacata aagcggttac tattaatagg agacaaatgc
//
LOCUS       DBERMR010000131          647 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.131, whole genome shotgun sequence.
ACCESSION   DBERMR010000131 DBERMR010000000
VERSION     DBERMR010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.131"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            41..>647
                     /locus_tag="ACL8A1_002188"
     CDS             41..>647
                     /locus_tag="ACL8A1_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGS1202868.1"
                     /translation="MGYSGFQELKLKMTKDILELESQKMSSDTFIDEIKNNYLSIVES
                     TRKLIDGRQIELAIKLIREANQILMIGVGSSGNAAREFESSLLRIGIISKTVIDTHFQ
                     LMHTALLKDNDLIIAFSLSGSTKEVEETLLNAKRKNVKIISITNYSSRNIAKLSDCVL
                     LTSKKESYLEGGSLMAKASQLFIIDVICTRLSLINYEDTICK"
BASE COUNT          259 a           66 c          117 g          205 t
ORIGIN      
        1 aaaagttgga gagggaactc ttaatcgatt ttgcaaaaag attggatact ctggttttca
       61 ggaactaaaa ttaaaaatga cgaaagatat attagagtta gaatctcaaa aaatgagtag
      121 tgacacattt attgatgaaa taaaaaataa ttatttatct attgtggaaa gtacaagaaa
      181 attaattgat ggtagacaaa tagaattagc tataaaatta ataagagaag caaaccagat
      241 tttaatgatt ggtgtgggtt ctagtggaaa tgcggctaga gaatttgaga gtagtttgtt
      301 aagaattgga ataataagta agacggtaat tgatacccat tttcaactca tgcatactgc
      361 gttattgaaa gataatgatt tgataatagc gtttagttta tctggttcca caaaagaagt
      421 tgaggaaact ttattaaacg caaaaagaaa aaatgttaaa attatttcaa taacaaatta
      481 ttcgtccaga aatatagcaa aattatctga ctgcgttctt ttgacatcaa aaaaagaatc
      541 ttatttagag ggtggtagtt taatggctaa ggcttctcaa ctattcataa ttgatgtaat
      601 atgcactaga ctctctttaa taaattatga agatactata tgtaaaa
//
LOCUS       DBERMR010000132          631 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.132, whole genome shotgun sequence.
ACCESSION   DBERMR010000132 DBERMR010000000
VERSION     DBERMR010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.132"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            94..390
                     /locus_tag="ACL8A1_002189"
     CDS             94..390
                     /locus_tag="ACL8A1_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HGS1202869.1"
                     /translation="MKSKRMEFHNKFLIVSAMLAVISWLSLGVVSFPMLAGTLGISTK
                     AAATVVNLISAYSTVTAVISIVGAITGVGSIGSGIAATVLYILKKKGAAKAALW"
     gene            397..>631
                     /locus_tag="ACL8A1_002190"
     CDS             397..>631
                     /locus_tag="ACL8A1_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1202870.1"
                     /translation="MKILSKKECESILGYFNFIGITLLFFVIISLSIYFKSYLPFQGK
                     LFLLFLVVDYATLNRYNMKLPIQSKKINTFYIRK"
BASE COUNT          217 a           78 c          111 g          225 t
ORIGIN      
        1 atatagacga acatttgaag agagttagga atacaacatg tgcgaattaa ctctcaataa
       61 aacatacata atatacaaaa ggagtacact aaaatgaaat ctaaaagaat ggaatttcat
      121 aataaatttc ttattgtttc agcaatgtta gcagtcatta gttggttaag cttaggtgtt
      181 gtttcttttc ctatgttggc tgggacatta ggaatttcga caaaagctgc tgctactgta
      241 gtcaatttga ttagtgctta tagtacagtt acagcagtaa tttcaattgt aggtgcaatc
      301 acaggagtag gttctattgg ttcgggtatt gcagcaacag ttctttatat attgaaaaag
      361 aaaggtgctg ctaaagcagc tctatggtag aactagatga aaatactttc taaaaaagaa
      421 tgtgaaagta ttttaggtta ttttaatttt attgggataa cccttttgtt ttttgttatc
      481 ataagtttgt caatctattt taaaagctat cttccttttc aagggaaatt atttttactt
      541 tttttggtag tagattacgc tactttgaat cgttataata tgaaactgcc tatccaaagt
      601 aaaaaaataa atactttcta tataaggaaa a
//
LOCUS       DBERMR010000133          591 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.133, whole genome shotgun sequence.
ACCESSION   DBERMR010000133 DBERMR010000000
VERSION     DBERMR010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.133"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            37..>591
                     /locus_tag="ACL8A1_002191"
     CDS             37..>591
                     /locus_tag="ACL8A1_002191"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGS1202871.1"
                     /translation="MIDSKVFIDATGNATIASASGADVYEKNSSYGCLMRVGGVDISK
                     TIEHIEKTKQWNYIEGYNQWLEEVLVNRKYRATGAVLKDPVSYDHAPMLSKDDHFMND
                     EKWNYIKERWEKFRFIYTLEVSLFRNELKKAVESGDFNFEIYHDDKSGVTMNGDGISY
                     GSWGQNVALINVAKAFGFNPKYMED"
BASE COUNT          221 a           64 c          120 g          186 t
ORIGIN      
        1 aaaagtcttg aaattttaag taatcaggaa gttaaattga ttgattctaa agtttttatt
       61 gatgctacag ggaatgcaac aattgcttct gctagtggag ctgatgttta tgaaaaaaat
      121 tcttcttacg gttgtttaat gagagttgga ggagttgata tctcaaagac tatagaacat
      181 attgaaaaaa caaagcagtg gaactatata gaaggatata atcagtggtt agaagaagta
      241 ttagtaaatc gaaagtacag agccactgga gcagttttaa aagatccagt ttcttatgat
      301 catgccccta tgctttcaaa agacgaccat tttatgaatg atgaaaaatg gaattacatt
      361 aaagaaagat gggaaaaatt tagatttatt tacactttag aagtatcact atttaggaat
      421 gaattaaaaa aagctgtaga atctggggat tttaattttg aaatttatca tgatgataaa
      481 tctggagtca ctatgaatgg cgacggtata agctatggtt catggggtca aaatgtagca
      541 ttaattaatg ttgcaaaagc atttggattc aatcccaaat atatggaaga t
//
LOCUS       DBERMR010000134          590 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.134, whole genome shotgun sequence.
ACCESSION   DBERMR010000134 DBERMR010000000
VERSION     DBERMR010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.134"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(7..486)
                     /locus_tag="ACL8A1_002192"
     CDS             complement(7..486)
                     /locus_tag="ACL8A1_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HGS1202872.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLW
                     LDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
BASE COUNT          158 a          141 c           80 g          211 t
ORIGIN      
        1 gacttcctag tgggtacgga tagtaaactc cttaaacaca atgcgtaagt ctcttaatac
       61 tctttgacgc cctcgaaatc gttcattgct taagacgcgt tctagttctt cttgtttctc
      121 tttactttgt ttgtttctat aatctcttag tgtttcatga aagagataat aatcatctag
      181 ccagagaccc ccctcactta tacgatgact gatctcaccc acttcttcat agggttcttt
      241 atcgtatcta cgcttctgac tttcctgctt acggatatag tctaaaattc gatttcgaaa
      301 cttggttttg aaatatttcc ttaaacgtgg aatatcgtct accagtcctt cttctctact
      361 aatcaattca tgtaagcaga gcatgccttc ttggtcccaa tccgacaatt cccataaatg
      421 aaggtaatat tcatttctac acttatacac agtcccttgg acttcttcat acaattcttt
      481 aatcataatt ttcccctttc tagatacagt ctaacagatt agaaaacaca tttgttccat
      541 tttctccatt ctgttccctt tagctcctaa actgcacaaa aaagagaggc
//
LOCUS       DBERMR010000135          558 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.135, whole genome shotgun sequence.
ACCESSION   DBERMR010000135 DBERMR010000000
VERSION     DBERMR010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.135"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            48..524
                     /locus_tag="ACL8A1_002193"
     CDS             48..524
                     /locus_tag="ACL8A1_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGS1202873.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEQLFDQVASLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPGK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAEATA"
BASE COUNT          185 a           94 c          109 g          170 t
ORIGIN      
        1 agtgttatac ttatggcata aagttaatgg aaagaaggga aaaccttatg gtattagatt
       61 atttctttga caaaaacctt gtgttttgct tagaagcgga taatcaagaa caactctttg
      121 atcaggtagc aagcttgttg gaagaacgag aaattgtcac tcctacttat cgtgaagcct
      181 tgatcacgcg tgaaaagtca tttccaactg gtttagatat ggaatttcta ggaaaggact
      241 tgccaaatgt agcgattcct cacacagata ttgttcacaa tctggctgaa aaagttgtgg
      301 tcgttcgatt agagaaaccg gtgactttcc ataatatgat tgcgccaggt aaggaagtag
      361 aagtatcctt gctcttcttt atcatcaata attcaagttc aagtcaaaca aatattctgg
      421 ctcagttgat ggactttttc acaggaaatg gtcatcttga agacctatca aaaatttctg
      481 aaccagaaaa actttatgct tacattgctg aagcaaccgc ttaatcttgt ctattaaaaa
      541 ataaaatcgg aggaaatc
//
LOCUS       DBERMR010000136          480 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.136, whole genome shotgun sequence.
ACCESSION   DBERMR010000136 DBERMR010000000
VERSION     DBERMR010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.136"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..>480
                     /locus_tag="ACL8A1_002194"
     CDS             <1..>480
                     /locus_tag="ACL8A1_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choline-binding protein A"
                     /protein_id="HGS1202874.1"
                     /translation="KKVAEAEKKVEEAKKKAEDQKEEDRRNYPTNTYKTLELDIAESD
                     VKVKEAELELVNEEAKPRNEEKIKKAKAKVESEKAEAIRLEEIKTDREEAKRKADAKL
                     KEAVEKNAANSEQGEPKRRVKRGVLGEPATPDKKENDAKSSDSSVGEETLPSPSLKP"
BASE COUNT          203 a           77 c          122 g           78 t
ORIGIN      
        1 aaaaaaaggt tgcagaagct gagaagaagg ttgaagaagc taagaaaaaa gccgaggatc
       61 aaaaagaaga agatcgccgt aactacccaa ccaatactta caaaacgctt gaacttgaca
      121 ttgctgagtc cgatgtgaaa gttaaagaag cggagcttga actagtaaac gaggaagcta
      181 aacctcgaaa cgaggaaaaa attaagaaag caaaagcgaa agttgagagt gaaaaagctg
      241 aggctataag gttagaagaa atcaagacag atcgtgaaga agctaaacga aaagcagatg
      301 ctaagttgaa ggaagctgtt gaaaagaatg cagcgaattc agagcaaggt gaaccaaaga
      361 ggcgggtaaa acgaggagtt cttggagagc cagcaacacc tgataaaaaa gaaaatgatg
      421 cgaagtcttc agattctagc gtaggtgaag aaactcttcc aagcccatcc ctgaaaccag
//
LOCUS       DBERMR010000137          445 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.138, whole genome shotgun sequence.
ACCESSION   DBERMR010000137 DBERMR010000000
VERSION     DBERMR010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.138"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..>445
                     /locus_tag="ACL8A1_002195"
     CDS             <1..>445
                     /locus_tag="ACL8A1_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choline-binding protein A"
                     /protein_id="HGS1202875.1"
                     /translation="RLEKIKTDRKKAEEEAKRKAAEEDKVKEKPAEQPQPAPAPQPEK
                     PAEEPNPAPAPKPEKPADQPKAEKPADQQAEEDYARRSEEEYNRLTQQQPPKPEQPAP
                     APKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNSNGAMATGW"
BASE COUNT          184 a          106 c           94 g           61 t
ORIGIN      
        1 aaggttagaa aaaatcaaga cagatcgtaa aaaagcagaa gaagaagcta aacgaaaagc
       61 agcagaagaa gataaagtta aagaaaaacc agctgaacaa ccacaaccag cgccggctcc
      121 tcaaccagaa aaaccagctg aagagcctaa tccagctcca gctccaaaac cagagaagcc
      181 agctgatcaa ccaaaagcag aaaaaccagc tgatcaacaa gctgaagaag actatgctcg
      241 tagatcagaa gaagaatata atcgcttgac tcaacagcaa ccgccaaaac cagagcaacc
      301 agctcctgca ccaaaaacag gctggaaaca agaaaacggt atgtggtact tctacaatac
      361 tgatggttca atggcgacag gatggctcca aaacaatggc tcatggtact acctcaacag
      421 caatggcgct atggcgacag gatgg
//
LOCUS       DBERMR010000138          440 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.139, whole genome shotgun sequence.
ACCESSION   DBERMR010000138 DBERMR010000000
VERSION     DBERMR010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.139"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..>440
                     /locus_tag="ACL8A1_002196"
     CDS             <1..>440
                     /locus_tag="ACL8A1_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HGS1202876.1"
                     /translation="YLKDAAHADNIRTKEEIKRQKQERSHNHNSRADNAVAAARAQGR
                     YTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKNELSASELAAAEAYWNGKQGSRP
                     SSSSSYNANPAQPRLSENHNLTVTPTYHQNQGENISSLLRELYA"
BASE COUNT          142 a           93 c           93 g          112 t
ORIGIN      
        1 taccttaagg atgcagctca tgcggataat attcggacaa aagaagagat taaacgtcag
       61 aagcaggaac gcagtcataa tcataactca agagcagata atgctgttgc tgcagccaga
      121 gcccaaggac gttatacaac ggatgatggg tatatcttca atgcatctga tatcattgag
      181 gacacgggtg atgcttatat cgttcctcac ggcgaccatt accattacat tcctaagaat
      241 gagttatcag ctagcgagtt agctgctgca gaagcctatt ggaatgggaa gcagggatct
      301 cgtccttctt caagttctag ttataatgca aatccagctc aaccaagatt gtcagagaac
      361 cacaatctga ctgtcactcc aacttatcat caaaatcaag gggaaaacat ttcaagcctt
      421 ttacgtgaat tgtatgctaa
//
LOCUS       DBERMR010000139          411 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.140, whole genome shotgun sequence.
ACCESSION   DBERMR010000139 DBERMR010000000
VERSION     DBERMR010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.140"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            6..143
                     /locus_tag="ACL8A1_002197"
     CDS             6..143
                     /locus_tag="ACL8A1_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HGS1202877.1"
                     /translation="MAEEVLNLQLVSVQVDETDEVDGMRFSTFSTNRCGNWSAFSWEN
                     C"
BASE COUNT          124 a           52 c           95 g          140 t
ORIGIN      
        1 tagctatggc agaagaagta ttaaatcttc aattagtatc tgttcaagtt gacgaaacag
       61 atgaagttga tggaatgcga tttagtacat tcagtacaaa ccgttgcggt aactggagtg
      121 cttttagttg ggaaaattgt taattcattt aaattgtaaa ctttagggat tctctgaagt
      181 tatggatgtc gttccaatat gtgcacgttg gaatgttagt gcttatatgg ggggaacaga
      241 atcctctctt gattgaagac aagttagcca ttaggctggt ttgtctcttt gtcaactgta
      301 gtgggttgat ataatagtat tagtgagtgg gataaaagtt tcatttagtt tattcagtac
      361 aaatttaacg ggtcaagatt tattgaactg caccccaata gttagacaga a
//
LOCUS       DBERMR010000140          358 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.141, whole genome shotgun sequence.
ACCESSION   DBERMR010000140 DBERMR010000000
VERSION     DBERMR010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..358
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.141"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(4..93)
                     /locus_tag="ACL8A1_002198"
     tRNA            complement(4..93)
                     /locus_tag="ACL8A1_002198"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(55..57),aa:Ser,seq:tga)
     gene            complement(176..249)
                     /locus_tag="ACL8A1_002199"
     tRNA            complement(176..249)
                     /locus_tag="ACL8A1_002199"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(213..215),aa:Ile,seq:gat)
     gene            complement(284..354)
                     /locus_tag="ACL8A1_002200"
     tRNA            complement(284..354)
                     /locus_tag="ACL8A1_002200"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(320..322),aa:Gly,seq:tcc)
BASE COUNT          108 a           97 c           78 g           75 t
ORIGIN      
        1 aaaaggagga tgtgggattc gaacccacgc acgcttttac acgcctgacg gttttcaaga
       61 ccgttccctt cagccggact tgggtaatcc tccaaaatag tttatacggg aacaagcccg
      121 tgtcctctta gtgaacagat aatattccaa tcccctagta atggactaaa cactatgggc
      181 acgagtggac tcgaaccacc gacctcacgc ttatcaggcg tgcgctctaa ccacctgagc
      241 tacgcgccca agtcaaaaac ttggtacaaa gaacaaagtt caaagcgggt gacgagaatc
      301 gaactcgcga caacagcttg gaaggctgta gttttaccac taaactacac ccgctaaa
//
LOCUS       DBERMR010000141          341 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.142, whole genome shotgun sequence.
ACCESSION   DBERMR010000141 DBERMR010000000
VERSION     DBERMR010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.142"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..>341)
                     /locus_tag="ACL8A1_002201"
     CDS             complement(<1..>341)
                     /locus_tag="ACL8A1_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGS1202878.1"
                     /translation="SEIIAYNLSTSPNLEQVKSMLEQAFTEKHYENTILHSDQGWQYR
                     HDSYHRFLESKGIQASMSRKGNSPDNGMMESFFGILKSEMFYGFEKSFQSLKQLEQAI
                     IDYIDYYNNKR"
BASE COUNT           92 a           71 c           66 g          112 t
ORIGIN      
        1 attcgtttat tgttgtagta atcaatatag tctataatag cttgttccaa ttgcttaagc
       61 gactgaaacg acttctcaaa accgtaaaac atttccgatt tcagaatccc aaagaaggac
      121 tccatcatac cattgtctgg gctgttaccc ttacgtgaca tggatgcttg aattccctta
      181 ctctctagga accgatgata agaatcgtgt cggtattgcc agccttggtc actatggaga
      241 atcgtattct cgtagtgctt ctctgtaaag gcctgttcta acatactctt cacttgttct
      301 aagttgggcg aagttgaaag attataggcg ataatttcgc t
//
LOCUS       DBERMR010000142          339 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.143, whole genome shotgun sequence.
ACCESSION   DBERMR010000142 DBERMR010000000
VERSION     DBERMR010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.143"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
BASE COUNT          138 a           48 c           51 g          102 t
ORIGIN      
        1 aatatatcag atggggtgca aaattatatt attttttaaa aacgcttaca aattgtaagg
       61 gtgggtaaaa actcaatttc aggagaaaat gaagtgaata tttccacact aaaacgcata
      121 atatcaaggt tttttaggct ctataatatt tgtagtgggt aaatccacta tagatattat
      181 ggagcctatt ttattgtaga aaaaaaatcc cataagatct ataatgaaaa gcgaccaaac
      241 aactcattag aaagattcat atgggacaat tacattttat cacaaaacta ctagacatta
      301 aagaccctaa tatccagatt ttagacatca tcaataagg
//
LOCUS       DBERMR010000143          299 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC49 isolate 457394
            SAMN38106831-rid24763233.denovo.144, whole genome shotgun sequence.
ACCESSION   DBERMR010000143 DBERMR010000000
VERSION     DBERMR010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38106831
            Sequence Read Archive: SRR26673342
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570568.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570568.1
            Genome Coverage       :: 154x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:39:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,202
            CDSs (total)                      :: 2,157
            Genes (coding)                    :: 2,015
            CDSs (with protein)               :: 2,015
            Genes (RNA)                       :: 45
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 142
            CDSs (without protein)            :: 142
            Pseudo Genes (ambiguous residues) :: 0 of 142
            Pseudo Genes (frameshifted)       :: 80 of 142
            Pseudo Genes (incomplete)         :: 70 of 142
            Pseudo Genes (internal stop)      :: 52 of 142
            Pseudo Genes (multiple problems)  :: 53 of 142
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38106831-rid24763233.denovo.144"
                     /strain="GPSC49"
                     /isolate="457394"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(94..222)
                     /locus_tag="ACL8A1_002202"
     CDS             complement(94..222)
                     /locus_tag="ACL8A1_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative RiPP precursor"
                     /protein_id="HGS1202879.1"
                     /translation="MKYCKPTFESIATFKKDTKGLWTGKFRDIFGGRAIVRIRIEF"
BASE COUNT          108 a           53 c           31 g          107 t
ORIGIN      
        1 aaaaaacaaa gcatcttgat attcgtagtt ctggaatgaa tatcaaaata ctttattcaa
       61 aattatttac cattgtaata catagttata agcttaaaac tcaattctaa ttcttacaat
      121 tgcacgtcca ccaaagatat cacgaaactt accagtccat agacctttag tgtctttttt
      181 gaaagttgca attgattcaa atgttggttt gcaatatttc atcttaaact ccttctatca
      241 tttatactaa tgccagaacc cattcttata aatgaaaaat atttttcaag agatatctc
//
